LOCUS       DAZUKZ010000001        95644 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000001 DAZUKZ010000000
VERSION     DAZUKZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 95644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.001"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..301
                     /locus_tag="ACG8EF_000001"
     CDS             <1..301
                     /locus_tag="ACG8EF_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS4945366.1"
                     /translation="KKMFKSRDGITLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFN
                     VSDKGISSARKLLYVICSRAKNNLFLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            501..815
                     /locus_tag="ACG8EF_000002"
     CDS             501..815
                     /locus_tag="ACG8EF_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4945367.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            836..1213
                     /locus_tag="ACG8EF_000003"
     CDS             836..1213
                     /locus_tag="ACG8EF_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4945368.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1223..1996
                     /locus_tag="ACG8EF_000004"
     CDS             1223..1996
                     /locus_tag="ACG8EF_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945369.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2018..3421
                     /locus_tag="ACG8EF_000005"
     CDS             2018..3421
                     /locus_tag="ACG8EF_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS4945370.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3603..4787
                     /gene="mobT"
                     /locus_tag="ACG8EF_000006"
     CDS             3603..4787
                     /gene="mobT"
                     /locus_tag="ACG8EF_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS4945371.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4784..5074
                     /locus_tag="ACG8EF_000007"
     CDS             4784..5074
                     /locus_tag="ACG8EF_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945372.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5071..5292
                     /locus_tag="ACG8EF_000008"
     CDS             5071..5292
                     /locus_tag="ACG8EF_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945373.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5334..6113
                     /locus_tag="ACG8EF_000009"
     CDS             5334..6113
                     /locus_tag="ACG8EF_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFS4945374.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6174..6674
                     /locus_tag="ACG8EF_000010"
     CDS             6174..6674
                     /locus_tag="ACG8EF_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS4945375.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            6730..7368
                     /locus_tag="ACG8EF_000011"
     CDS             6730..7368
                     /locus_tag="ACG8EF_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFS4945376.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7440..7835
                     /locus_tag="ACG8EF_000012"
     CDS             7440..7835
                     /locus_tag="ACG8EF_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4945377.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            7819..10272
                     /locus_tag="ACG8EF_000013"
     CDS             7819..10272
                     /locus_tag="ACG8EF_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4945378.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10269..12296
                     /locus_tag="ACG8EF_000014"
     CDS             10269..12296
                     /locus_tag="ACG8EF_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS4945379.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12293..13315
                     /locus_tag="ACG8EF_000015"
     CDS             12293..13315
                     /locus_tag="ACG8EF_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFS4945380.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13332..14261
                     /locus_tag="ACG8EF_000016"
     CDS             13332..14261
                     /locus_tag="ACG8EF_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4945381.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14575..14622
                     /locus_tag="ACG8EF_000017"
                     /pseudo
     CDS             14575..14622
                     /locus_tag="ACG8EF_000017"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14638..15438
                     /locus_tag="ACG8EF_000018"
                     /pseudo
     CDS             14638..15438
                     /locus_tag="ACG8EF_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            15676..15912
                     /locus_tag="ACG8EF_000019"
     CDS             15676..15912
                     /locus_tag="ACG8EF_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945382.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            15919..17871
                     /locus_tag="ACG8EF_000020"
     CDS             15919..17871
                     /locus_tag="ACG8EF_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS4945383.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(17943..18440)
                     /gene="dfrG"
                     /locus_tag="ACG8EF_000021"
     CDS             complement(17943..18440)
                     /gene="dfrG"
                     /locus_tag="ACG8EF_000021"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS4945384.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            18715..19842
                     /locus_tag="ACG8EF_000022"
                     /pseudo
     CDS             18715..19842
                     /locus_tag="ACG8EF_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            19940..20128
                     /locus_tag="ACG8EF_000023"
     CDS             19940..20128
                     /locus_tag="ACG8EF_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFS4945385.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(20186..20539)
                     /locus_tag="ACG8EF_000024"
     CDS             complement(20186..20539)
                     /locus_tag="ACG8EF_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4945386.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            21070..21492
                     /locus_tag="ACG8EF_000025"
     CDS             21070..21492
                     /locus_tag="ACG8EF_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4945387.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21489..21719
                     /locus_tag="ACG8EF_000026"
     CDS             21489..21719
                     /locus_tag="ACG8EF_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4945388.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            22217..22417
                     /locus_tag="ACG8EF_000027"
     CDS             22217..22417
                     /locus_tag="ACG8EF_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFS4945389.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            22444..23637
                     /locus_tag="ACG8EF_000028"
     CDS             22444..23637
                     /locus_tag="ACG8EF_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4945390.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(23705..25270)
                     /gene="guaA"
                     /locus_tag="ACG8EF_000029"
     CDS             complement(23705..25270)
                     /gene="guaA"
                     /locus_tag="ACG8EF_000029"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS4945391.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25329..26252)
                     /gene="coaA"
                     /locus_tag="ACG8EF_000030"
     CDS             complement(25329..26252)
                     /gene="coaA"
                     /locus_tag="ACG8EF_000030"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFS4945392.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26448..27053
                     /locus_tag="ACG8EF_000031"
     CDS             26448..27053
                     /locus_tag="ACG8EF_000031"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4945393.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27361..28377
                     /locus_tag="ACG8EF_000032"
     CDS             27361..28377
                     /locus_tag="ACG8EF_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4945394.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            28518..29819
                     /locus_tag="ACG8EF_000033"
     CDS             28518..29819
                     /locus_tag="ACG8EF_000033"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFS4945395.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            29865..30527
                     /gene="deoC"
                     /locus_tag="ACG8EF_000034"
     CDS             29865..30527
                     /gene="deoC"
                     /locus_tag="ACG8EF_000034"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFS4945396.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            30548..30946
                     /locus_tag="ACG8EF_000035"
     CDS             30548..30946
                     /locus_tag="ACG8EF_000035"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFS4945397.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            31012..32094
                     /locus_tag="ACG8EF_000036"
     CDS             31012..32094
                     /locus_tag="ACG8EF_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4945398.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            32306..33868
                     /locus_tag="ACG8EF_000037"
     CDS             32306..33868
                     /locus_tag="ACG8EF_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4945399.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            33861..34994
                     /locus_tag="ACG8EF_000038"
     CDS             33861..34994
                     /locus_tag="ACG8EF_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4945400.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            34994..35950
                     /locus_tag="ACG8EF_000039"
     CDS             34994..35950
                     /locus_tag="ACG8EF_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4945401.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            36049..37215
                     /gene="deoB"
                     /locus_tag="ACG8EF_000040"
     CDS             36049..37215
                     /gene="deoB"
                     /locus_tag="ACG8EF_000040"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFS4945402.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            37239..38057
                     /locus_tag="ACG8EF_000041"
     CDS             37239..38057
                     /locus_tag="ACG8EF_000041"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS4945403.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            38074..38781
                     /gene="deoD"
                     /locus_tag="ACG8EF_000042"
     CDS             38074..38781
                     /gene="deoD"
                     /locus_tag="ACG8EF_000042"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS4945404.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(39079..39765)
                     /gene="gpmA"
                     /locus_tag="ACG8EF_000043"
     CDS             complement(39079..39765)
                     /gene="gpmA"
                     /locus_tag="ACG8EF_000043"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS4945405.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(39937..40518)
                     /locus_tag="ACG8EF_000044"
     CDS             complement(39937..40518)
                     /locus_tag="ACG8EF_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4945406.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(40651..41334)
                     /gene="rpiA"
                     /locus_tag="ACG8EF_000045"
     CDS             complement(40651..41334)
                     /gene="rpiA"
                     /locus_tag="ACG8EF_000045"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFS4945407.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            41653..42066
                     /gene="rpsL"
                     /locus_tag="ACG8EF_000046"
     CDS             41653..42066
                     /gene="rpsL"
                     /locus_tag="ACG8EF_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFS4945408.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            42150..42620
                     /gene="rpsG"
                     /locus_tag="ACG8EF_000047"
     CDS             42150..42620
                     /gene="rpsG"
                     /locus_tag="ACG8EF_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFS4945409.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            42714..44801
                     /gene="fusA"
                     /locus_tag="ACG8EF_000048"
     CDS             42714..44801
                     /gene="fusA"
                     /locus_tag="ACG8EF_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFS4945410.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            44973..46160
                     /gene="tuf"
                     /locus_tag="ACG8EF_000049"
     CDS             44973..46160
                     /gene="tuf"
                     /locus_tag="ACG8EF_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS4945411.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            46567..46875
                     /gene="rpsJ"
                     /locus_tag="ACG8EF_000050"
     CDS             46567..46875
                     /gene="rpsJ"
                     /locus_tag="ACG8EF_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFS4945412.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            46906..47535
                     /gene="rplC"
                     /locus_tag="ACG8EF_000051"
     CDS             46906..47535
                     /gene="rplC"
                     /locus_tag="ACG8EF_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFS4945413.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            47563..48186
                     /gene="rplD"
                     /locus_tag="ACG8EF_000052"
     CDS             47563..48186
                     /gene="rplD"
                     /locus_tag="ACG8EF_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFS4945414.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            48186..48476
                     /gene="rplW"
                     /locus_tag="ACG8EF_000053"
     CDS             48186..48476
                     /gene="rplW"
                     /locus_tag="ACG8EF_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFS4945415.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            48515..49348
                     /gene="rplB"
                     /locus_tag="ACG8EF_000054"
     CDS             48515..49348
                     /gene="rplB"
                     /locus_tag="ACG8EF_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFS4945416.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            49391..49669
                     /gene="rpsS"
                     /locus_tag="ACG8EF_000055"
     CDS             49391..49669
                     /gene="rpsS"
                     /locus_tag="ACG8EF_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFS4945417.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            49692..50039
                     /gene="rplV"
                     /locus_tag="ACG8EF_000056"
     CDS             49692..50039
                     /gene="rplV"
                     /locus_tag="ACG8EF_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFS4945418.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            50053..50709
                     /gene="rpsC"
                     /locus_tag="ACG8EF_000057"
     CDS             50053..50709
                     /gene="rpsC"
                     /locus_tag="ACG8EF_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFS4945419.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            50712..51146
                     /gene="rplP"
                     /locus_tag="ACG8EF_000058"
     CDS             50712..51146
                     /gene="rplP"
                     /locus_tag="ACG8EF_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFS4945420.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            51136..51324
                     /gene="rpmC"
                     /locus_tag="ACG8EF_000059"
     CDS             51136..51324
                     /gene="rpmC"
                     /locus_tag="ACG8EF_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFS4945421.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            51349..51615
                     /gene="rpsQ"
                     /locus_tag="ACG8EF_000060"
     CDS             51349..51615
                     /gene="rpsQ"
                     /locus_tag="ACG8EF_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFS4945422.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            51673..52041
                     /gene="rplN"
                     /locus_tag="ACG8EF_000061"
     CDS             51673..52041
                     /gene="rplN"
                     /locus_tag="ACG8EF_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFS4945423.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            52078..52386
                     /gene="rplX"
                     /locus_tag="ACG8EF_000062"
     CDS             52078..52386
                     /gene="rplX"
                     /locus_tag="ACG8EF_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFS4945424.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            52414..52953
                     /gene="rplE"
                     /locus_tag="ACG8EF_000063"
     CDS             52414..52953
                     /gene="rplE"
                     /locus_tag="ACG8EF_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFS4945425.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            52972..53157
                     /locus_tag="ACG8EF_000064"
     CDS             52972..53157
                     /locus_tag="ACG8EF_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFS4945426.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            53193..53591
                     /gene="rpsH"
                     /locus_tag="ACG8EF_000065"
     CDS             53193..53591
                     /gene="rpsH"
                     /locus_tag="ACG8EF_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFS4945427.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            53623..54159
                     /gene="rplF"
                     /locus_tag="ACG8EF_000066"
     CDS             53623..54159
                     /gene="rplF"
                     /locus_tag="ACG8EF_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFS4945428.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            54320..54676
                     /gene="rplR"
                     /locus_tag="ACG8EF_000067"
     CDS             54320..54676
                     /gene="rplR"
                     /locus_tag="ACG8EF_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFS4945429.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            54697..55197
                     /gene="rpsE"
                     /locus_tag="ACG8EF_000068"
     CDS             54697..55197
                     /gene="rpsE"
                     /locus_tag="ACG8EF_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFS4945430.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            55212..55391
                     /gene="rpmD"
                     /locus_tag="ACG8EF_000069"
     CDS             55212..55391
                     /gene="rpmD"
                     /locus_tag="ACG8EF_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFS4945431.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            55435..55875
                     /gene="rplO"
                     /locus_tag="ACG8EF_000070"
     CDS             55435..55875
                     /gene="rplO"
                     /locus_tag="ACG8EF_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFS4945432.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            55875..57170
                     /gene="secY"
                     /locus_tag="ACG8EF_000071"
     CDS             55875..57170
                     /gene="secY"
                     /locus_tag="ACG8EF_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFS4945433.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            57232..57879
                     /locus_tag="ACG8EF_000072"
     CDS             57232..57879
                     /locus_tag="ACG8EF_000072"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFS4945434.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            58070..58288
                     /gene="infA"
                     /locus_tag="ACG8EF_000073"
     CDS             58070..58288
                     /gene="infA"
                     /locus_tag="ACG8EF_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFS4945435.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            58321..58437
                     /gene="rpmJ"
                     /locus_tag="ACG8EF_000074"
     CDS             58321..58437
                     /gene="rpmJ"
                     /locus_tag="ACG8EF_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFS4945436.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            58455..58820
                     /gene="rpsM"
                     /locus_tag="ACG8EF_000075"
     CDS             58455..58820
                     /gene="rpsM"
                     /locus_tag="ACG8EF_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFS4945437.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            58848..59237
                     /gene="rpsK"
                     /locus_tag="ACG8EF_000076"
     CDS             58848..59237
                     /gene="rpsK"
                     /locus_tag="ACG8EF_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFS4945438.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            59317..60255
                     /locus_tag="ACG8EF_000077"
     CDS             59317..60255
                     /locus_tag="ACG8EF_000077"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFS4945439.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            60283..60663
                     /gene="rplQ"
                     /locus_tag="ACG8EF_000078"
     CDS             60283..60663
                     /gene="rplQ"
                     /locus_tag="ACG8EF_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFS4945440.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            60935..61774
                     /locus_tag="ACG8EF_000079"
     CDS             60935..61774
                     /locus_tag="ACG8EF_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4945441.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            61750..62619
                     /locus_tag="ACG8EF_000080"
     CDS             61750..62619
                     /locus_tag="ACG8EF_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4945442.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            62609..63406
                     /locus_tag="ACG8EF_000081"
     CDS             62609..63406
                     /locus_tag="ACG8EF_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS4945443.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            63609..64355
                     /gene="truA"
                     /locus_tag="ACG8EF_000082"
     CDS             63609..64355
                     /gene="truA"
                     /locus_tag="ACG8EF_000082"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS4945444.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(64688..67357)
                     /locus_tag="ACG8EF_000083"
     CDS             complement(64688..67357)
                     /locus_tag="ACG8EF_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFS4945445.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(67595..67783)
                     /gene="rpmB"
                     /locus_tag="ACG8EF_000084"
     CDS             complement(67595..67783)
                     /gene="rpmB"
                     /locus_tag="ACG8EF_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFS4945446.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            68053..68415
                     /locus_tag="ACG8EF_000085"
     CDS             68053..68415
                     /locus_tag="ACG8EF_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4945447.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            68467..70149
                     /locus_tag="ACG8EF_000086"
     CDS             68467..70149
                     /locus_tag="ACG8EF_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFS4945448.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            70266..72302
                     /gene="recG"
                     /locus_tag="ACG8EF_000087"
     CDS             70266..72302
                     /gene="recG"
                     /locus_tag="ACG8EF_000087"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFS4945449.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            72351..73352
                     /gene="plsX"
                     /locus_tag="ACG8EF_000088"
     CDS             72351..73352
                     /gene="plsX"
                     /locus_tag="ACG8EF_000088"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFS4945450.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            73474..73716
                     /gene="acpP"
                     /locus_tag="ACG8EF_000089"
     CDS             73474..73716
                     /gene="acpP"
                     /locus_tag="ACG8EF_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS4945451.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            73984..74988
                     /locus_tag="ACG8EF_000090"
     CDS             73984..74988
                     /locus_tag="ACG8EF_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4945452.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            74989..75933
                     /locus_tag="ACG8EF_000091"
     CDS             74989..75933
                     /locus_tag="ACG8EF_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4945453.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            75933..76895
                     /gene="opp4B"
                     /locus_tag="ACG8EF_000092"
     CDS             75933..76895
                     /gene="opp4B"
                     /locus_tag="ACG8EF_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS4945454.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            76922..77836
                     /locus_tag="ACG8EF_000093"
     CDS             76922..77836
                     /locus_tag="ACG8EF_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4945455.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            77862..79643
                     /locus_tag="ACG8EF_000094"
     CDS             77862..79643
                     /locus_tag="ACG8EF_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4945456.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            79991..80677
                     /gene="rnc"
                     /locus_tag="ACG8EF_000095"
     CDS             79991..80677
                     /gene="rnc"
                     /locus_tag="ACG8EF_000095"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFS4945457.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            80697..84278
                     /gene="smc"
                     /locus_tag="ACG8EF_000096"
     CDS             80697..84278
                     /gene="smc"
                     /locus_tag="ACG8EF_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFS4945458.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            84287..85096
                     /locus_tag="ACG8EF_000097"
     CDS             84287..85096
                     /locus_tag="ACG8EF_000097"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4945459.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            85107..86105
                     /gene="ftsY"
                     /locus_tag="ACG8EF_000098"
     CDS             85107..86105
                     /gene="ftsY"
                     /locus_tag="ACG8EF_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFS4945460.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            86292..88559
                     /gene="gshAB"
                     /locus_tag="ACG8EF_000099"
     CDS             86292..88559
                     /gene="gshAB"
                     /locus_tag="ACG8EF_000099"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFS4945461.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(88648..89100)
                     /locus_tag="ACG8EF_000100"
     CDS             complement(88648..89100)
                     /locus_tag="ACG8EF_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFS4945462.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            89250..90200
                     /locus_tag="ACG8EF_000101"
     CDS             89250..90200
                     /locus_tag="ACG8EF_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4945463.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            90193..91152
                     /locus_tag="ACG8EF_000102"
     CDS             90193..91152
                     /locus_tag="ACG8EF_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4945464.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            91149..91904
                     /locus_tag="ACG8EF_000103"
     CDS             91149..91904
                     /locus_tag="ACG8EF_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4945465.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            91927..92880
                     /locus_tag="ACG8EF_000104"
     CDS             91927..92880
                     /locus_tag="ACG8EF_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4945466.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(93524..93820)
                     /locus_tag="ACG8EF_000105"
     CDS             complement(93524..93820)
                     /locus_tag="ACG8EF_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFS4945467.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            94175..94531
                     /locus_tag="ACG8EF_000106"
     CDS             94175..94531
                     /locus_tag="ACG8EF_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4945468.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            94541..95440
                     /locus_tag="ACG8EF_000107"
     CDS             94541..95440
                     /locus_tag="ACG8EF_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4945469.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        31483 a        17005 c        20123 g        27033 t
ORIGIN      
        1 taagaagatg tttaaatctc gagatggtat cacacttact acagcacata aagttaaagg
       61 aaatgagtat gatactgtta tcgctattaa tcttctaaac gggaaaattc ctcattggaa
      121 tgaaattttt aatgtttctg ataaaggaat tagttcagcg agaaaactac tatacgtaat
      181 ttgttcaaga gcaaaaaata atttatttct tttctcagag gtgggttatt ttacgagtaa
      241 aggagacttg ctttatccca ctcaattatt agataaaata gaatttgatt ataatattta
      301 aaatacctag gtagtcgttg ttttaaatat agtaggaatc ggacgctcag tacggagcgt
      361 ctattttttt acccaatttt gaaaggaagt gaacttatga aaataaaaaa tcaagaatca
      421 aaaggtcgct ctcccccctt taaaaccatc acacaaataa gattcattca aacataaaaa
      481 cagaaaggat aggtaaaaat atggaactta aatatgtcat tcccaatatg gaaaaaacat
      541 ttggacaatt agaatatgct ggtgaaggga acattgaaca acgtcgaata aatggcagac
      601 caaccattct ctctcgtagt tacaatttat actcaacgat tcaacgagca gatgatattg
      661 ttgtcattct tccctccgaa gctggcgaga aacattttga atccgacgaa aaagtcaaac
      721 tcatcaaccc acatattacc gcagaaggtt ataaaattgg caatcgagga tttaccaatt
      781 acattatgca cgcagacgat atggtgaaag cataagaaag gaaaggtgaa aaaatatgag
      841 attagcagaa ggtattgtga ttgaaaaaga ccagacattt ggcacattga aattttccgc
      901 cttgcgtcgt gaggttcgta tccagaatga agatggtacg gtttccgaag aaattaaaga
      961 acgtacctat gacttaaaat caaaaggaca aggacgcatg atacaagtca gtatccccgc
     1021 ttctgttcct ttgaaagaat ttgactacaa cgcagaagtg gaattaatca atcctgtggc
     1081 agataccgta gcgacggcta cttttcgagg ggcagacgtt gattggtacg tgaaagcaga
     1141 cgatcttgta ttaaaaggac agacaaaacc tcatcagaaa tataaaaaag aagcgtccga
     1201 aagtgaaaaa taggtgttgc ttatgacaaa cacaccattg attttgaaag aaattccgaa
     1261 ggatgaagcc atttctttta ttcggcaata ccattattca aagatactcc ctagattgtg
     1321 taaatacttt ctagggattt tttcagaaga aaaattatta ggcgttgtgg aattaggctg
     1381 gggtacgcaa cccctacaaa ccatacgcaa actctttccc gacagttccc ttcaaaccac
     1441 agattattta gaaattggaa agatgtgttt tcttccagaa atgaatcaaa ccaattattt
     1501 tggcagtcaa gctctttcag ctctaattaa gtggttgaaa gaacatacgg actgtcattt
     1561 tttatataca ctggcagatg gaattgaggg aaaatgtggc tatgtctatc aagcgtccaa
     1621 cttcttttac tgcgggtatt tcaagacaag tgtctatcgt gataagcagt catgggaaaa
     1681 gatacaccct cgaagtgctc gccttttatt agaggaaaat gctcgctttg aacaggtaga
     1741 gaaaaagcat tggctttccc aggcattttg cgaatacaaa gggattgaga aaatcaatgg
     1801 acgcatgttt cgctatctct atccgttgac aaaagaagcg aaaaaactgt taggacacac
     1861 tctctatcga cgtcattatt atccgaaaga aaaaaattta cgctttgaaa agcgaatcgc
     1921 ttatcagaaa tatgaagcca tttctcaacc aacttttgat aaacaggctc gtatctacaa
     1981 tacacaatta ttttgatagg tggaaaggag gatttttatg ccattgcttc atgacagagg
     2041 agagcgaatc aaagccaacg ataagcactt ggtctatcat tttgtactaa tttcattgct
     2101 ggtagttttt ctagtcgtcc tactcccttt tcattggaga gcgttacaat cggtgaactg
     2161 gcaagcagtt cagttagata catttttcta tcaacttcat acgccatact tttggagcag
     2221 tattctcact gctctttttg tatgcggact tatcagctgg ctttattatc actatcgtat
     2281 agattctata aaacagttag agcaccgtca aaagttagct cgtatgattt tagaaaatca
     2341 ttggtatgaa gccgaagaaa ctcaatcaga gggattcttt aaagatttac ccagcaagaa
     2401 aaccaaacaa cgaatccgcc attttcctaa aatatattat cgaatgaaag atggactaat
     2461 ccatattcat gtagaaatca ccttagggaa atatcaagat cagcttttgc acctagaaaa
     2521 caaactagaa acaggattgt attgtgaatt agtggaaaaa acgttgaaag attcttatgt
     2581 agagtatgtc ctactttatg atactattgg caatcgaatt ggcattgaag acgtggtagc
     2641 tcaagatgga cgtgtcaaat tgatggaatc ggtctattgg gagttcgatt ctctccctca
     2701 catgttaatt gctgggggca ctggtagtgg aaaatcctat tttattctca cattgattga
     2761 atctctcctg catacaaatg caaagcttta tattcttgac cctaaaaatg ctgatttggc
     2821 agacttaggg gcggtcatga ataatgtcta ttaccgtaaa gaagatatgt tggcatgtat
     2881 caatcaattt tacgaagata tgattgatcg cagtgaaacc atgaaacaac accctaacta
     2941 taaaacagga gaaaattatg cttatcttgg tttgtctgcc aattttctta tttttgatga
     3001 atatgtggca atgatggata tgctaggtcg tgaaagtacc accgttattc ataaactcaa
     3061 acaaattgta atgttaggac gacaagcagg cttcttttta attcttgcct gtcaacgtcc
     3121 agacgcaaaa tattttagtg atgggattcg ggacaacttt aacttccgtg tggctttggg
     3181 tcgtatgtct gaaatgggct ttggtatgat gttcggaagc gatacacaaa aacaattttt
     3241 cttaaaaccc atcaagggaa gaggttacgt ggatacagga aaaagtgtga ttagtgagtt
     3301 ttatacaccc cttgtaccaa aaaggtacga tttcttagga gaaataggaa aagtcattca
     3361 aaaaaagcaa tctgagcctg taccgcacga agtgaaaggt acaggttcag acttgctttg
     3421 attggtgtgg cgttagccac gccaatcctt tagccccctc tatctaacag gggggcacaa
     3481 atacaactga aaacaggcaa ttctcatcaa ccaatgttga tggtacaaag gattgcggaa
     3541 aatatgaaag gtgtaaactc aagggtcata gtttacatct tttttagatt ggaggtttgc
     3601 gaatggaaaa cgaaaaaatt tggattaagg aattgaaaga aaagcgactg gcttatggcg
     3661 tttctcaaaa taaactggcg gttgcttctc atattaccag accttaccta tcggatattg
     3721 aaacaggcaa ggcagtccct actcaacagg tgaaagaaga tttactgaac gctttggaac
     3781 gcttcaatcc cgataaccca ttggagatgt tattcgacta tgtacggatt cgattcccta
     3841 ccaatgatgt ggctcaaatt atcggtgagg ttttacgttt gaatatggat tatatgctac
     3901 acgaagactt tggctattat tcttatcccg aacactatcg tttcggtgat attgttgtcc
     3961 tagtctctca tgatgtaagt aaaggtgttt tattagaact gaaaggaaaa ggctgtcggc
     4021 aatttgaaaa ctttctattg gctcaacatc ggagctggta tgatttcttt caagattgca
     4081 tggaacataa aggtattttc aaacgcttgg atttagctat caacgataaa acagggattt
     4141 tgaatattcc agatttggca aagaaatgta aacaggaaga atgcatttcc gtctttcgaa
     4201 gctttaaaaa ttatcgcagt ggtgaattgg ttcaccgtga tgaaaaacca tacatgggaa
     4261 acacacttta tattggctca ttaaaaagtg aagtctattt ctgtatatat gagaaagatt
     4321 atgagcaata cgtgaaaaat gatgttccct tagaagacgc agaagtaaag aatcggtttg
     4381 aaattcggtt aaaaaatgac cgagccaccc aagccatgaa agatttattg gcacatcaac
     4441 aggcggaaaa aacagccttt gaaattatca atcgctatat ccgctttgca gataaagacg
     4501 acactaaacg tcggagtgat tggaaaacta atgaacgttg ggaatggttt atcggtaaaa
     4561 atcgtggagc attacgccta accacacagc cagagcctta ttcttttgaa cgaacattga
     4621 actggttaca tcatcaagtc gcccctacat taaaaatcgc aagcatttta gatgttctca
     4681 acggtacaac cattatctcc accatgattc aggaagccaa actgacagaa aaacatgaga
     4741 aattaattga acaacaacat ctagccatgg aggatttgat tacatgagcc ttttaacaaa
     4801 agaattaaag aagttagggt ttcagtgtgg cattgagttt caagcgtata tacaaaatac
     4861 agggaaatat acgtctctca ttattgaggg aaaacggcaa gctggtgata ccatctatac
     4921 ctatgatttt tacaaggtga gattttataa taattatacc aatcgagtaa ccgtctatgg
     4981 agagcattta accccttttc aattattaag gcgtgtaaaa agctatatct attatcgaga
     5041 aaaatatctt aaggaaagga ggacaatcac atgaattttg ggcagaattt gtacaactgg
     5101 ttcttggata atgcacagtc attggtattg ttagcgattg ttgtcattgg actgttttta
     5161 ggttttaaac gagaattttc aaaactcatt ggcttcttga ttattgctct aatcgccgtc
     5221 ggacttgtgt tcaatgcagc tggcgtgaaa gatgtgttat taaacttatt taaccgaatc
     5281 attggtgctt aacagatatt tttcatgata aaataggaag tatatatttt gttatgaaag
     5341 gatttatgaa tatgtttaca aatgatcaat ttaaatctct tttaattaaa acattagaaa
     5401 gaaagagctc ttcaaattat tatgaaggag gtaacgagat tgtttctaaa atgaaaatat
     5461 cagaggtaac tttagatgag actgacgatt ttacttatta taaaggatat aaaaagggct
     5521 ggaatacgct ttgcacatat cttaaaataa gagttccttt tgatgattta gacttttttg
     5581 aaagtcataa agatttgatt acccagacag caagctccat atatgataaa caaggtgata
     5641 atgtattagt ggatactatt ttagtaccac ttccagagaa ttacgaagta attaacttta
     5701 gtcagttgaa aattagtgat gtggtttcac aagctattga agacgctgag tcttttatga
     5761 gtaatggtga atatcaacgt gcctttgaca gagttcacac tgcttttcat ggatatttaa
     5821 ttgaaatact aaaaaaatat gaaattacag ttcctagaga cgaaaatcta tctaaattat
     5881 atagtaggat acaacaatta attgaaaaag aaattcaacc tactgaactt gctgatatag
     5941 tgaagacaac gataaggtca tcaaatggaa tgattagctc tcttaatgaa gcaagaaata
     6001 ggcattcact tgcacaccct aatacaaata ttattggaaa gagagaagca aaactaataa
     6061 taggtatttc aagtacagtt acagattata ttagcggata ccttgataag tagcgtcatt
     6121 aactgattgc tacttttttt atgactattt ttataaaaag gatgtgataa gaattggaaa
     6181 tgaaagtcta tgtggcaaat ttaggacggt ataatgaagg tgaattagtc ggagcatggt
     6241 ttactccacc aattgatgag gaagaaatgg cagaaagaat cggtttaaat gaggactacg
     6301 aagaatatgc aatacatgat tttgagcttc cttttgatgt ggacgaatac acgccgatta
     6361 gtgaaatcaa ccgtctatgt gaagccattc aagaaatcga aggtacacct atttataatg
     6421 aactcaaaga aattcaaggc atgtggttta gtagcttgga agaattactg gaaaacaaag
     6481 aggatattca ttgttatagt gattgtgatt cgatggaaga tgtcgcccgt tattacgtgg
     6541 aagaaacagg acagcttggt gaagtccctt ccaatttaca aaattatatt gactaccaat
     6601 ctttgggacg tgatatggaa atagagggta attacctagt tacctctcat ggggtatttg
     6661 aatattgtca gtaagtttaa caatccgtgt tataattaaa ttaataaata gcacggagag
     6721 gtataaatta tggtaaatgt atttgaaaat cttaaactat taaaactaga catgaaagat
     6781 aaaggctggg tgatagattc cttttacttt cgttacaaac agcaaaatta tattgtactt
     6841 gttaagctat ttgaaaaaga ggaaaaagtt ccagaatatg ctctgttaaa actagaattt
     6901 ttaaaggaaa acgatttttc agatatgcta gctgtctatg ctaattcggt aaaattatat
     6961 acagacacga aaacaataag agaatatttt ggtatcgaat acagtagtaa cttgggagat
     7021 gttttatttc aatttagcca aactcttgct cgatttattc cgacagaagt tagtgagaag
     7081 aaaaatgaag accaaaaaga agccatgtgt ttctcattaa gtcaaagtga ttcagaagat
     7141 cccagaaaaa aatattgttt ttcagttaga agaaatcctt taaagggaaa tggtgagctg
     7201 ggaaaacgaa gtccttttaa tgacaataaa acgagattgt atagacccag cttatatgaa
     7261 cgattagggt cagataccaa tttaagtttc agatattcaa tgaatccaga tgatgaagaa
     7321 actgatgaag gtattattgc aaaatggaca aaaaataaaa ttgaatagtt aaagcattga
     7381 aagagcaatc acctattttg attgttcttt ttctattttt acggaaagga cgtgaagaca
     7441 tgaaaaaaat aagaagttac accagtattt ggtcagtaga aaaagtgatt tatgcgatta
     7501 atgatttaca actccctttc ccaattacct ttacgcaaat gacatggttt atcgtttctt
     7561 tgtttgtcgt catactcttt ggcagtttac caccactttc tctgattgac ggagcactac
     7621 tcaaatactt agggattccc attgttctca ctgggttcat gtgccaaaag acctttgacg
     7681 gaaagaaacc ctatggattt ctaaagtcta tcattaccta tgctctacgc cctaaagtaa
     7741 cctacgctgg caaacaaata cgattaaaaa aagaaacaat gaatgaatcg attacgattg
     7801 taaggagtga acaactagat ggcatatccg attaaatata tggaagataa tctcgtattt
     7861 aatcatgatg gcgaagtatt cgcttattat gaattaattc cctataatta cagtttttta
     7921 agttccgatg aaaaaattca agttcatgat aatttctgtc aattgattgc ccaaaatcgt
     7981 gatggcaaga ttcatgctct gcaactagca accgaatcaa gtattcgagc cacacaggaa
     8041 caatcgaaaa aagaaatcac tggacgatta aaagagatag cttacgaaaa aatagatgaa
     8101 cagaccgaag cgttaatttc tatgattggt gacaatcaag tagactatcg cttttttatt
     8161 ggttttaaac tctacctcaa tgaacaggaa gtatccgtaa aaaatgttgg gaaagaaatt
     8221 caaaaagcat ttgcggaatt ctttcatggt gtgaatcaca aattaatggg cgattttgtt
     8281 tctatgccta tggcggaaat cgaacgcttt tctaagatgg aacagttgtt agaaagtaaa
     8341 ttatccagac gctttcagat tcgtaaattg gagaaagatg attttggtta tattattgaa
     8401 catctttacg ggcaaacagg tgtccctttt gatgactacg actaccattt accgaaacga
     8461 tacgaagata aaaaagtgtt ggtaaagaaa tacgatattc tcaaacccac tcgctgttta
     8521 atcgaagaaa atcaacgcta tttacggatt gaacaggaag ataccactac ttatgtggcg
     8581 tattttacaa ttaatagtat tgttggtgaa ttggatttcc ctaatagtga aattttttat
     8641 taccagcaac agcagtttac tttccccgtt gataccagta tgaatgttga aattgtcgcc
     8701 aataaaaaag ccttatctac cgttcgcaat aaaaagaaag aactgaaaga tttagatagc
     8761 cacgcatggg aaagcgacaa cgaaaccagt actaatgtgg tggacgcttt agaatctgtc
     8821 gatgaattag aaagtaattt agaccaaacc aaagaatcta tgtacaaact aagctatgtg
     8881 gtacgggtat cagcaaagaa tttggacgag ctgaaacaac gttgtgatga agtaaaagat
     8941 ttttatgatg atctgtctat caaattagta cgaccttttg gcgatatgct aggcttgcat
     9001 aatgaattta ttccctcaag caagcgatat atcaatgact atattcaata tgtcacgtcg
     9061 gattttttgg ctggtcttgg tttcggtgct acacaaatga ttggtgaaaa ttctggtatt
     9121 tacgtcggtt acaatctcga tacaggcaga aatatttatc tccaacctag ccttgcaagt
     9181 caaggaatta aaggctctgt aacaaatgca ttagcgtccg cttttattgg ttcattaggt
     9241 ggcggaaaat cattctctaa caatatgctg gtctattatt cagtactctt tggcggacag
     9301 gcaaccattg ttgacccaaa agctgaacgt ggtgaatgga aagaaacctt acctgatatt
     9361 gcccatgaaa tcaatattgt taatctcacg agtgagaacg acaataaagg gttacttgac
     9421 ccttatgtga ttttaaaact cccgaaagat tcagaaagcc ttgccattga tattctcacg
     9481 ttcttaacag gtatttccag ccgagatagc gagaagtttc ctgtcctgcg taaagctatt
     9541 cggagtgtaa ctcaaagcaa acaacgaggg ttgttattgg tgattgatga attgcgaaaa
     9601 gaagatacgc ccatcagcaa tagtattgct gaccatatcg aaagctttgt cgattatgat
     9661 tttgcccatc tgttattcag tgatggcagt gtcacccaat ccattagctt ggaaaaacaa
     9721 ctcaatatta ttcaagtggc agatttggtc ttacccgatt ccaatagctc ttttgaagaa
     9781 tacactacca tggaattatt aagtgtggca atgctgattg tcattagtac ctttgcttta
     9841 gactttatcc actctgaccg ttccattttt aaaattgttg accttgatga ggcttggagt
     9901 tttttacaag tggcacaagg aaaagctcta tctatgcgat tggttcgagc aggacgtgcc
     9961 atgaatgcag gggtctattt cgttactcaa aacgcggacg atttactaga tgaaaagatg
    10021 aagaataata ttggcttgaa atttgccttt cgttccactg acctagtgga aatcaaaaag
    10081 accttagaat tttttggtgt ggacaaggaa gatgaaaaca atcagaaacg cttacgtgaa
    10141 ttagaaaatg gacaatgttt agtttcagac atctatggtc gtgtaggggt tatgcagttt
    10201 cacccgattt ttgaagagtt gcttcatgcc tttgatacca gaccgcctgt gcgaaatgag
    10261 gtggaagaat gacacaaaaa caaaagaaat ttcttcacta tagttggata acgctccttt
    10321 gtgtgggcgt tattttactt ttattaggca cgctgggcaa tgcagtacaa gcgacaggct
    10381 tagttgatga aaccattgat acaagcaatg aatactccaa gtatggtttg aatcaatatc
    10441 agcttgatta ttatgtggac aatagctggg gctggcttcc atggaattgg agtgatggca
    10501 taggacaatc ggtgatgtat ggattgtatg ccattactaa ttttatttgg acaatttctc
    10561 tttatctatc gaacgccaca ggttatttgg tgaaagaagc ctatgcctta gatttcatta
    10621 gtcaaacatc ccaagcaatt ggcgaaaaca tgcagacgat tgcaggcatt acaccgaatg
    10681 gcttttccag ttcagggttt tatgtcggtt tcctccttct ttttattctc attgttggaa
    10741 tctatgttgc ctatacagga ttggttaagc gagaaacgac gaaagtgata cgagctgttt
    10801 taaactttgt cgtggtgttt attctatcgg cttcctttat tgcgtatgca cccgattata
    10861 ttgcgaaaat caatgatttt tccaaagatg tcagtaccgc cagtctgaat attgggacaa
    10921 aaattgttct tcctaacacg gatagtcaag ggaaagacag tgtggacatg atacgtgaca
    10981 gcttattctc tgttcaagta aagcaaccat ggctattgct tcaatatggc tccactgatg
    11041 tagaagcgtt aggggaagaa cgtgtggaaa gtttattgtc taccagtcca gatacaaaca
    11101 atggtgaaga tagagaaaat gtagtgattg aagaaattga agatcatgac aatacctatt
    11161 taaccctacc gaagacgatt tccagattag gaacagtctt ctttttgttt gtattcaata
    11221 ttggtatctc gatttttgtt ttccttctaa caggaatcat gattttttcg caagtgctat
    11281 ttattatttt cgctatgttt ttgccaatca gtttcctatt aagtatgttg ccgagttttg
    11341 atggcatggg caggcatgcc attaccaagt tatttaatgt gattatgaca agggctggta
    11401 ttaccttgat tattaccgtt gcctttagta tttccactat gctttattcg ctgtcagcaa
    11461 gttctccctt tttcatgatt atgtttttac aaattgtgac ctttgcaggg atttatttta
    11521 aactgggcga tttaatgagc ctattcactt tacaaagcaa cgactctcaa aacatgggca
    11581 gacaagtgtt tcgcagacct cgtcaaatga tgaatcgaca aaccagaaga tttcaacgca
    11641 gtatgagtaa gatttttcgt ggaaaagcag ggtcaacgaa gcaaaagaca atgacaacaa
    11701 agcagaatca aaccaataaa gctaaccata ctcgtcaaaa tgaacggact acaccaaaga
    11761 aagaaacagc actttccaat gtgaaaaagc aacaaggcgg tatcgggcaa cgattaggtg
    11821 aaaagacagc ccatgtattg gacacgaaaa atcgtctagt caataaaaga aagcaggcaa
    11881 aacaacagat acaggcgaca ccaacccatg tcaaatatgc ccttcatcaa ggaaaagaga
    11941 atgtcactca taatgtaaca gactttaaac aatctatggc agataccaaa gctcaacgca
    12001 aacaacagcg tatgaaccag caggagcaaa aacgaaagaa cattgctcaa aagcgtttgg
    12061 agctggataa agcaaaagaa aaaagacgaa aagaaaaccc atcatctcct gttcagaata
    12121 accagcagca tgttcgcaag cgtcagcaaa ccgtcacgcc tgttttgtca caaaacacta
    12181 aacgtgaaaa tcatcagcga ccattgaaaa aagagcaaaa acaaaagaca ccaccgaagc
    12241 aattcaatca gaggaagcta aataaaagaa cggataagcg aggacaacaa acatgaagct
    12301 aaaaataaca gtgatttgta gtgtggtctt ctctctcttc ttttttctct tattattcct
    12361 agtcattttc tttgcagatg atacggacag tggcgaaaac aacaaggatt cttctattcc
    12421 acaaggcggt gtgaccgttt caccagaagt gttggcacac cgtccgttga ttgaaaaata
    12481 tggtaaggaa tatgggattg aagactatgt ttcttatatc ttagccatta tgcaagtgga
    12541 atcgggcggt acagcagaag atgttatgca aagttccgaa tctttgggtt taccgcccaa
    12601 cagtttgagt accgaagagt ccatcaaaca aggggttaaa tatttcagtg aattattaac
    12661 cagtgcagaa caacaaggag tggatataga ttctgttatc caatcttata attatggcgg
    12721 tggcttttta aattatgtga ggagtcatgg gaaaaaatat acctatgaat tagccgaaca
    12781 gttttctaaa gagaagtcgg gtggtcaaaa agcagactat cccaatccta tagccatacc
    12841 tgtgaatggt ggttggcgat ataactatgg aaatcagttt tatgttcagt tggtttcaca
    12901 atacctaacc gacacttccc caacagaatt tgatgatgaa accgtgcaag tcattatgga
    12961 cgaagccttg aaatacgaag ggtttcccta tgtctttgga ggggcgtcgc ctaccacctc
    13021 ttttgattgt agtggcttaa tacaatgggt gtatgacaaa gctggcattt ctctccctcg
    13081 tgtcgcccaa gatcagtatg acgctacgca agaaatctct atggaagaag cccaagcagg
    13141 cgaccttatc ttctttcatt caacctataa tgcaggcaca tatgtgactc atgtggcgat
    13201 atatttagag ggaaatcgct tttatcatgc aggcgaccca atcggttatg gcgacttaag
    13261 cagtcgttat tggcaagacc atctgattgg ggctagacgt gttattcata actaagaaag
    13321 gaatgaatca aatgattata aaaatcgagc gaaaagaaaa gaaggagaaa ccaaagaaat
    13381 ctaaaaaacc gagcaagcaa agaaaagttc cgatgattaa agttggcacg cataagaaat
    13441 taacctttgt tttatgggtt ttattgattg gtagtgtcgg atttggaatc tataaaaatt
    13501 ttactgccat tgacactcat actgtccgtg aaacagaaat cattaaacag cagattgtcg
    13561 ataccaacca agtagaaagt tttgtgaaat cctttgcgaa agactacttt tcgtggcaac
    13621 aatcccaaga agccattgat aaacgaaatg aaaaattgac ccattatttg acggaagaat
    13681 tgcaggtatt aaatgaagaa atgattcgta aggatattcc tactagttct agtgtgaacg
    13741 atatacaggt gtggcaggtt tctcaagtga atgagaacac ctttgaggta ctgttttctg
    13801 ttgaacaagt tattaccgaa gacaaagaca aggaaactat atcgtctagc tttcatgtgg
    13861 ttgtccatat agatgaatca gataatatgg ttattatcaa aaatccgaca atgagtaaga
    13921 aacctcaaaa atcggactac caacccaaac agctagaaag tgaccataca gtagatacag
    13981 aaacgatgga cgaaatcact agctttttag aaacgttctt ccagctctat cctaccgcca
    14041 cagaaaaaga attgacttac tatgtaagta atcatgtgtt acctatgatt aacaaggaat
    14101 atgttttcga ggaattggtg aatcctatct ttaccagaaa agataaccaa gtaatcgtga
    14161 atgtcgctgt gaaatattta gatcaagaaa caaaggcaac ccaaatctcg caatttgagt
    14221 tgatattaga aaaacaagat aattggaaga ttgtaaaata acaaatattg gtacatgatt
    14281 acagatactt tgtaatcatg tactcttttt gataaaaaat tggagattcc tttacaaata
    14341 tgctcttacg tgctattatt taagtgacta tttaaaagga gttaataaat atgcggcaag
    14401 gtattcttaa ataaactgtc aatttgatag cgggaacaaa taattagatg tcctttttta
    14461 ggagggctta gttttttgta cccagtttaa gaataccttt atcatgtgat tctaaagtat
    14521 ccagagaata tctgtatgct ttgtatacct atggttatgc ataaaaatcc cagtgataaa
    14581 agtatttatc actgggattt ttatgccctt ttgggttttt gaatggagga aaatcacatg
    14641 aaaattatta atattggagt tttagctcat gttgatgcag gaaaaactac cttaacagaa
    14701 agcttattat ataacagtgg agcgattaca gaattaggaa gcgtggacaa aggtacaacg
    14761 aggacggata atacgctttt agaacgtcag agaggaatta caattcagac aggaataacc
    14821 tcttttcagt gggaaaatac gaaggtgaac atcatagaca cgccaggaca tatggatttc
    14881 ttagcagaag tatatcgttc attatcagtt ttagatgggg caattctact gatttctgca
    14941 aaagatggcg tacaagcaca aactcgtata ttatttcatg cacttaggaa aatggggatt
    15001 cccacaatct tttttatcaa taagattgac caaaatggaa ttgatttatc aacggtttat
    15061 caggatatta aagagaaact ttctgccgaa attgtaatca aacagaaggt agaactgtat
    15121 cctaatatgt gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt aatagaggga
    15181 aacgatgacc ttttagagaa atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa
    15241 caagaggaaa gcataagatt tcagaattgt tctctgttcc ctctttatca tggaagtgca
    15301 aaaagtaata tagggattga taaccttata gaagttatta ctaataaatt ttattcatca
    15361 acacatcgag gtccgtctga actttgcgga aatgttttca aaattgaata tacaaaaaaa
    15421 agacaacgtc ttgcatatta agtagttcag ttttggagta caaaaagtga aaatgtaaac
    15481 ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt aaatcaacgt
    15541 ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg cgaacgtgaa
    15601 ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac taagcaaaaa
    15661 ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa gtctgtggat
    15721 tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata tgcagagcgt
    15781 actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa gaagtttatt
    15841 gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg tatcaaagat
    15901 agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga agatgaaatt
    15961 gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa acaatatgat
    16021 ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt gccaacgtct
    16081 tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa tccgttttgt
    16141 tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc aagtcgatat
    16201 aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa tgacaatcct
    16261 attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg gtctgttgtt
    16321 gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc agattatcaa
    16381 tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc gaagcaattt
    16441 tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc gtgtaagaag
    16501 ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca aaagaacacc
    16561 attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg tacttgtgat
    16621 attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta taggcgttgt
    16681 ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt taacgagatg
    16741 tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa aggacaaggt
    16801 tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt tgtatctgct
    16861 gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa tatttcaatg
    16921 gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac ttttagtcgt
    16981 aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa cgatagcgaa
    17041 aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag atttgtcgat
    17101 ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat taaacaaatg
    17161 gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag aaacgccttt
    17221 tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat ttgccaagtt
    17281 aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa agcagaactg
    17341 ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt ggcatctcat
    17401 tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag taaagatgga
    17461 gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac taaggataaa
    17521 ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa cgaaatcgca
    17581 aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca gataagacgt
    17641 tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa gagctatatt
    17701 tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag aacttactac
    17761 aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc tcaaagatta
    17821 gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg acaaaaagag
    17881 cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa ttaacgcacc
    17941 cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata cggattttta
    18001 tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt cacttctgga
    18061 aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt ctcaacataa
    18121 gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc ttcgttttta
    18181 cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa ggtaaacccc
    18241 ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat tgattcaagg
    18301 ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata ttcccagtcc
    18361 ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt atccatcgca
    18421 gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc atctaatttc
    18481 ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata agcaattttt
    18541 tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa ttatcatttt
    18601 caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa atgaaaaaag
    18661 tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta cttacttgca
    18721 tatatacgcc tttatagtgg agtactacat ttacgagatt cggttagagt atcagaaaaa
    18781 gaaaaaataa aagttacaga aatgtatact tcaataaatg gtgaattatg taagattgat
    18841 agagcttatt ctggagaaat tgttattttg caaaatgagt ttttgaagtt aaatagtgtt
    18901 cttggagata caaaactatt gccacagaga aaaaagattg aaaatccgca ccctctacta
    18961 caaacaactg ttgaaccgag taaacctgaa cagagagaaa tgttgcttga tgcccttttg
    19021 gaaatctcag atagtgatcc gcttctacga tattacgtgg attctacgac acatgaaatt
    19081 atactttctt tcttagggaa agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag
    19141 tatcatgtgg agatagaact aaaagagcct acagtcattt atatggagag accgttaaaa
    19201 aatgcagaat ataccattca catcgaagtg ccgccaaatc ctttctgggc ttccattggt
    19261 ttatctgtat caccgcttcc gttgggaagt ggaatgcagt atgagagctc ggtttctctt
    19321 ggatacttaa atcaatcatt tcaaaatgca gttatggaag ggatacgcta tggttgcgaa
    19381 caaggattat atggttggaa tgtgacggat tgtaaaatct gttttaagta cggtttatac
    19441 tatagccctg ttagtactcc agcagatttt cggatgctta ctcctattgt actggagcaa
    19501 gcctttagaa aagctggaac agaattgtta gagccatatc ttagttttaa agtttatgca
    19561 ccacaggaat atctttcacg ggcatataac gatgctccca aatattgtgc aaatatcgta
    19621 aatactcaac tgaaaaataa tgaggtcatt attattggag aaattcctgc tcgatgtatt
    19681 caagattatc gcaatgattt aacttttttt acaaatgggc ttagtgtttg tttagcagag
    19741 ctaaaaggat atcaggttac cactggcgaa cctgtttgcc agacccgtcg tctaaatagt
    19801 cggatagata aagtaagata tatgttcaat aaaataactt agtgcgtttt atgttgttat
    19861 ataaatatgg tttcttatta aataagatga aatattcttt aatatagatt tgaattaaag
    19921 tggaaaggag gagattgtta ttataaacta caagtggata ttgtgtccta tttgtggaaa
    19981 taaaacaaga ctacgaatac gagtggatac tatacttaaa aatttccctt tatactgccc
    20041 caaatgtaag aacgaaactt taattaatgt tcaaaaaatg aatataataa caatcaaaga
    20101 gccagacgcc aagacgcaga gccgataatt tgagaaatga aactctcatc ttatcggctc
    20161 tttttgttta tctgaatttt actgactagc cttcaatatt tcttgcttca ttgatacctt
    20221 ttgctagtgc ttccattaag gatagttctt tgtctgtaaa gctatccatg tatttttcta
    20281 cctgtaacct tcgagtgctt ttaatcaaat tatctgtagg taagaaaaat tcatcaatag
    20341 atacatggag taatgataca agatcataca gcacttgtgt acttgggtgt tgtcctttgt
    20401 tttctatatt tgttaaataa cgtgggtcaa tttcaatcat tgttcccact tgttcacgag
    20461 tgagaccccg tttcattcga gcctctttaa tcgctaaacc aacggctcta aaatcgtatt
    20521 tatcttcttt tttacgcata ctaaatcacc tctatacatt ttactgttcc tatataacca
    20581 taaacaggta tagaaaaaca tgttatatag tttatgagtt cctatagaac actgtggaat
    20641 ttattaatgc gatgaatcca atttcaaata gaaaggtcgc tgattttatt tacctataca
    20701 actttataaa atttagtcat actcctagaa agtagataaa attggcgact taatggaggg
    20761 aaaagtcgcc atgagaaaga taatctgtca tctagttctc ttaattaagt tcttatcaaa
    20821 aaaacttatc cacctgtact ggatagatat gaccatcaat gtggttttgc agattacata
    20881 gatagaaaaa tcaaagcata tgaacagaga aaacaatctg tttatgtgct tttttggtca
    20941 cttaataaat atcttttagg atttctcttc atttatgcaa caaatccccc gttcgtgtcg
    21001 gggtttagta tgaagggaga taaagaagca catgccactc cctttcacga cagaaagggg
    21061 gtgaaaagca tgaaaccatc ttcttttcaa gaaaccatag aaaatcagtt tgactatatc
    21121 tgcaaacaag ttattgaaga tgaacgaaaa gattacttca aacatctaga cagactatca
    21181 gcaaaagaag tctcgttttc tgatatagat aactaccttg tcaactcttt ttctactgtt
    21241 gatacgtacg tcactgattt ccaactattt aaattatttg gattaagaat caatgttgaa
    21301 agtgatttat taagtgaagc cctgcgtcat ctttcagaga aaaaacggaa cattatttta
    21361 cttcattact ttatggatat gagtgatgtg gagattgcgg agttactaaa tttgaatcgt
    21421 tcgactgttt atcgtcatcg aataagtgga ctagccatga ttaaagaatt catgaaggag
    21481 tgtaaagaat gaaaacagct tatccaaggg ttcccttccc cctcattgta aaggcgacag
    21541 atggcgacgt ggaagcaatt aatcagattg ttaaacatta tcgagggtat acctcaaaac
    21601 gttcattacg tcgcatgaca gacgaatatg gaaattctca tatggttatt gatgaaaccc
    21661 tacgtggcag aatggaaacg aaactcatct ctaagatttt atcgttcgaa attaaatagt
    21721 cccatttttt cgtggaagcg tgtcgaatcg tatccacgct tcccgatttg ggaggtttgt
    21781 tattccatca aagcatattg tgtgttcagt aggttttgat aggctaacaa gccaaacgtt
    21841 ctttgacaac caaataaagg caatcgaata cgtttcggta agaaaagagc tgatgaacta
    21901 acacgccatg acctacaaaa ttagtgagcg attcatgtta gtaattcgaa agtaatcttt
    21961 agcaggattg ctgccatgac tttcttatcg tgataatgat actcccgtat agccatagtc
    22021 cgagcgttag aagcgtcaca ggcaatgggt acggctacat gagaaccatg cagaagtgaa
    22081 attcctgtga gctttgctag agctgttcga ttgctaagat attcttctct tgtcagtgta
    22141 ttattataaa actatttgat ataattatag gtgaatattg gagttttcct agcctataat
    22201 tgggaaagga gtaataatga taacggtcac aaaaaaggat ttagttgagc taggatatgg
    22261 aacttctttt tcagcagaca tcataaaaaa agcaaaagaa ttgatgatta caaaagggca
    22321 tatctactat caatctcgaa agctagatag agtccctcgt gaagctgttg aagaaattct
    22381 aggaattcgg ttttcaaatg aaaacaggtc tgagtagttt ttgcacagta aggagtaaaa
    22441 actatggtta aagacccaat aaaaaaagca aagaatggaa cctattattt tcgtgcaaat
    22501 ctaggttatg attctaaagg gaaaaaaatt caaaaatatc gtagtggatt cactactaaa
    22561 aaagaagcaa gagaagaata ttcgaaactg ctattgatga aaccagaaga attaagtgag
    22621 aataaggata aaatgacgtt taaacattat atctttgaaa ttttccttcc atggtataag
    22681 acacaggtaa agctcagaac atatgaaaat cgcttaccaa ctattaagaa gcatttctct
    22741 tattttgata aaatggctgt ttccgatatt gagcctattg atgtgcaaaa ttggcaacta
    22801 aaattatcaa agaagtgtaa gtcttcttat gtaagagctg ttcaaggact attttcagtt
    22861 gctatggatc gagcaattgt ccttgggata accaccacaa atccatctaa gattattggc
    22921 aatgtgaaaa agcaaaagtc taaaattgaa ttttggacaa aagaagaatt tgaaaaagta
    22981 atctctctaa tttacaaaga agattactat caacactttt tatttatctc tctgtggttt
    23041 ttatttatga ctggaatgcg aattggtgaa gctactgcca ttcaatggga agatattgat
    23101 tttgactcag gagtactaac catcaacaaa acactctatt acaagaatca atcgaattat
    23161 agatttgttg aaccaaaaac aaaggcgagc gttcgtcata ttgtattaga taaatgcaca
    23221 ttaacttatt tatccgaatg gaaggaagta caacaaaatc taattcagac agactttgta
    23281 atgagctata acggtattcc tacccaaaaa catacactat catatgctat tgaacgctat
    23341 gctaagctgg ctggtatcca tcgaatcaga atccatgggt tacggcattc tcatgcttcc
    23401 ttgttaatca gtatgggaga aaatccactt atcattaaag atcgtctagg acacgaagat
    23461 attgagacga ctttaggaac gtatggacat ctttatccta acagtaattt cgaggtagct
    23521 aacaaattaa atggagccat ttcctttaaa gaagcaaaac aaaattatga tacttcacca
    23581 aagaatcaat ttactgtaga ctacttgcga aacaaagaaa tgaaaaaagt gcaataaaag
    23641 tgcagtaaaa agataagaag agccagaaac ccttggtata agaggatttc tagctctcct
    23701 acaactattc ccactccact gttgctggtg gtttagaagt gatgtcatac acgatacggt
    23761 tgacgtgggc tacttcgttt acgatacgta cagagatttt ttgcaagact tcccatggga
    23821 tacgggcaaa gtcagcggtc attccgtcga ttgaagttac agcacggata ccgactgtgt
    23881 agtcgtaagt acgtccgtca cccataacac cgacagagcg gatccctgga agaacagtga
    23941 aatattgcca gatatcacgg tcaagtccag cagctgcgat ttcttcacgc aagatagcat
    24001 cagattcgcg tacgatttct agcttgtctt cagtgatttc gccaagaaca cgaataccaa
    24061 gaccaggacc agggaatggc tgacgccaaa cgattgaatc aggcatgcct aactgagtcc
    24121 ctaattcacg gacttcgtct ttgaataatg tgtttaatgg ttcgatcaat tcaaattgca
    24181 tatcttctgg cagtccacct acattatggt gagatttgat cgtctgtgct gtttctgtac
    24241 cagattcgat aacgtcagta tacaacgttc cttgtgctag gaaagagacg ccttcttcac
    24301 cagcaagttt tgttgcttcg tcatcaaaaa catatacgaa ttcattccca atgattttac
    24361 gtttttgttc tgggtcagat acacctttca acttgtctaa gaagcgatct cttgcatcga
    24421 ctttgatgat gttcaagccg aattttccgc ctagactttc cattacttgt tccgcttcgc
    24481 ctttgcgtag cagaccgtgg tcaacaaaga tagatgtcaa ttggtcaccg atcgcttttt
    24541 gtaaaagaac cccaacgaca ctagaatcaa cgccacctga aagaccaagc aagacttttt
    24601 tgtcaccaac ttgttcacgg attttttcta cttgcatatc gataaagtta tccattgtcc
    24661 agttaccttc acagtgacaa acatcaaagg caaagtggcg cagcaattcg attccgtatt
    24721 cggaatgacg tacttctgca tggaattgga taccataaaa attacgtgtt gtgtcttgga
    24781 tagaagcaat cggacaatct ttgcttgttc cgactgtttc aaagccttca ggaactttag
    24841 ttaccaagtc accatggctc atccatactg tttgttgttt tggtgtttct ttaaacaaga
    24901 cagcttgatc gcttgttact tctaacaagg ctttcccata ttcgcggttt ttagcaggct
    24961 ctacggaacc gccaagtttg tacgtgatca attgcatacc atagcaaatc cctaatacag
    25021 gaatacctaa attgaagatc tcaggatcga tcccaaatgc attctcatca tatacactgt
    25081 ttggtccgcc agaaagaatg attccctttg gattcatcgc ttttacttct tctgcagtaa
    25141 tgcggtggct caataattcg gaaaagactc caagatcacg aatacgtctt gtgatcaatt
    25201 ggttatactg actaccgtag tctaatacga taatcttttc aacgttcgtc atatcggcaa
    25261 cgtttgtcaa atttattacc cctatccctt taaattttat ctcaaaatag tcaaactatt
    25321 ttaaaatgct aatattttcg catgaaaatc gaatcgatga tatgattttc agttttatgg
    25381 agaattatat cggctcttcc tcgtgtaggc aagatatatt cctgtaaatt tttcaaattc
    25441 acatttttcc atacatcttt tgccatttta aaggcttctt cacgatctcc gatagcaaaa
    25501 cgataataat aattctctgg attttggaaa gccgtatcca gtaacgcacc aaaacgatcc
    25561 aaataccatt tttcgatcag cacgggatcg gcatcgacat agacagaaaa atcaaaaaag
    25621 tcgctgacgt agatttgctg attcgcaggt aattgaagcg tattgatccc ctcaacgatc
    25681 aagatatcag gctgctggat caattcgtac ttgtctggaa tgatatcata cacatcatgt
    25741 gagtaaacag gtacttttat ctcttcctga ccagatttta cacggttcaa gaaatcgatc
    25801 agcatctcca tatcataact ttcaggaaat cctttacggt ccatgattcc acgctctttt
    25861 aatatacgat ttggataaag aaatccgtca gtcgtgatca gctgaacgtt tcttcgtttg
    25921 aaggtacgag acaaaatcgt ctgcagcagt ctggctgttg tactttttcc aacggctaca
    25981 cttccggcga tcccgataat aaaaggcggt acttctacgt atttatgaag aaataaacct
    26041 ttactcaaga acaaagattc aaattctttc atataaagat aaatcagatg agcaagaggt
    26101 acatagatat ctcgaacatc ctgcatggaa atgcggtcat tcaaactttt gatattatct
    26161 agttcatctt gtgtcaaagg tgcttgccca ttacgataaa attctcgcca ttcttctctt
    26221 gccacacggt agtagttcat tggatcattc atattagcct cctgcgttct cccatcaatg
    26281 gaactatttt aacacaaaat aaacgcaaac cccaccactt aagagagatt gccgatcttt
    26341 ttgtataatc tttataatag aaagaaacgg agtcttattc ttgacttgcg cacggataca
    26401 taaaaggata gactgttgtc atgaaagaaa tgagaaggat ggaagacatg acaaatcatt
    26461 actattctga acgcccagat acggcgcacg attttgaaca atgggctttt gagttaaaag
    26521 gcagaacctt tcaatttgta acagattctg gtgtattttc acgtgaaaca gtggattatg
    26581 gttcacgtgt cctgattgat gctttcgatt gggaagagtt acctgatgaa ggaaagatct
    26641 tagatgtggg atgcgggtat ggaccgattg ggttagcttt ggcatttgcg acacagcggt
    26701 ttgttgaaat ggtcgacatt aatgaacgag cggtaagtct agcgcaagga aacgcaaagc
    26761 gtaaccaaat cgaacatgtt gatatccatc aatcgaatat ctatgaagca gttcatgaag
    26821 aaacctatgc agctatcgta agcaatccgc cgatccgtgc tggaaaaaaa gttgtacatg
    26881 aaattttgac aggcgcatat cctcggttga agaagggcgg tacactgaca attgtgatcc
    26941 agaagaaaca aggagcgccg agtgcgcaaa agaaaatgga agaaacattt ggtaatgcag
    27001 aaatcgtcac aaaagataaa ggatattata tcatcaaaag tgtgaaagaa taactagctg
    27061 aaaaatagtg gagcgacgaa agagtggaac tgggcgtgca tgtacgggta cgtatcagtt
    27121 ctttttttgt tgagaagttt ttaaaaacaa agaactattt tttcttcaaa aagaaatagt
    27181 aggaacagag tcgttgtttt ttacgataaa ataaataaga tacagaactt gtatagaagg
    27241 ctgccaagtc ggtagataaa ggaggttttt atcaaaaaac atactttcct tttttctcta
    27301 ttcttttctt gggggttttg gtacactgat agggaattta gatagaaggg agccaatagg
    27361 atgaagctga ccatcagaga tatcgcagaa atggcaggcg tatcagtaac aactgtctca
    27421 cagattttga ataacaaagg aagcagattt agtgataaga caagaaaaaa agtgttgtcg
    27481 atcgtgaatg aacatcatta taagcccgat tatttcgctt caaatgtgat caatcgacat
    27541 tcaaagacca tcggaatgat cgtccctgat gtcactgatt tcttcttctc caaactgatc
    27601 gaaggtgtag agagctattt gaatcctttg ggctatgtga tcatgttatg caattctcgt
    27661 catagccaag aaaatgagat caagtattta caagagcttt cccaccgctc agtggacgga
    27721 atcttgctgg ccacaccaaa tattttacct gagaaatatg cattagacag cggtttttat
    27781 caaaaaatgc caatcatttt aatcgatcgc ggattgaatc gtcgggataa cggacggctt
    27841 atcgtaaagg aatatgaggg cgcctatcaa gctgtttcgc tgtttattga aaatggccat
    27901 acgaagatcg gcatgctgaa ggaaaccact ggatactatc aattagaaga acgtttcaac
    27961 ggatatcgtc atgcgctgaa agatcatggc ctgcctttta atgggaaatt tgttgaacat
    28021 ggagagttga ctgtccaagg tggatatact gcatcaaaaa aattactcaa acataaagat
    28081 atcacggcta ttttttgcgg gaatgatgcc atggcgatcg gatgctatca ggcgatcgat
    28141 gaattgggca aaaaaatccc agaagatatc tcagtcatag gattcgatgg attaaagctg
    28201 tcagaataca tgattccgca attgacgaca gtgcagcagc caagttttga tattggattt
    28261 tatgctgcac gtttcctgat cgatacgatc gaatttcctc aaagaaaagt tccgaacaaa
    28321 gtgttcgaaa caaaattaat tattcgtgag agcgtaaaag tattgacaaa gggctaactc
    28381 ggcggtatat tgcttatgta agcatttaca aaggctagtt agtgcttttt ttatttttag
    28441 taaaccagtt tactaaagtg gtttaccttt cctaaaaata atgtataata ccgaatgaag
    28501 acaaggagga tcaggcaatg cgaatggtgg atctgattga aaaaaagagg gatggaaacg
    28561 agctgtcgaa agaggaaatc gagtacatcg tcacgaacta tacgaatgga aagattccag
    28621 attatcaagt cagtgcatta ttgatggcaa ttttttatca agacatgaca aatgaagaaa
    28681 tcacgaactt aacattggcg attgctaatt ctggtgatgt catcgatttg agttctcttg
    28741 aaggaataaa ggtagataag cattcgacag gtggcgttgg tgacacgacc acattgattt
    28801 tagcccccct agtagctagt gtcggagtga cagtggctaa aatgtctgga cgcggtctag
    28861 gttacacagg tggtacacta gacaagctag aagcaattcc cggatttcag atcgagcttt
    28921 cggatgaaga atttgtacgc attgttaatg aaagtaaagt agcggtaatt ggtcaatcag
    28981 gaaacttggc accggcggac aaaaaattgt atgccttgcg tgacgtcaca gcgaccgttg
    29041 attctcttcc gctgattgcc agttcgatta tgagtaaaaa aatcgcggca ggagcagatg
    29101 caatcgtttt ggatgtgacc actggagacg gtgctttcat gaagaacatc gaagatgcaa
    29161 gacgtctggc aaagaccatg acgagcattg ggaaactggc taaccgagaa acagtggcgg
    29221 ttatctcaga tatgtcagaa cctcttggcg aagctatcgg aaatagtcta gaagttgtcg
    29281 aggcaatcga aacattacaa ggaaatggtc cagaagatct agtagaaatg tgctacgcct
    29341 taggaagcca aatggttgtt cttgctggca aagctaagac aaccgatgaa gcaagaacgt
    29401 tgcttcaaga agctctggaa tcaggtaaag cgttagcaaa attcaaagaa atgatccaaa
    29461 atcaaggggg agacccagcg atcgtggaac atccagaacg aattttgact gcacgctaca
    29521 caatggagct tcctgctaaa caaagcggag ttgtgtcaaa aatcgtggca aatgaattag
    29581 gaattgctgc aatgatgcta ggtgctgggc gtaaaaccaa agaagaagat attgatcacg
    29641 ctgttggatt gaaactgcat aaaaaaattg gtgacactgt gacaaaaggt gagtcactat
    29701 tgacgatcta tagtaacgac gaagaaatca cttctgtcat tgaattatta tataaaaata
    29761 ttgaaattgg tgaatcggca atgaagccaa cgttgattca tgacatcatc acagaataaa
    29821 ggaaaaaagc ggactttacc gttttaaaga aaaggggaaa agaaatggaa ctaaaccgaa
    29881 tgatcgacca tactattttg aaagcagacg catctaaaga agctgtgatg caaattatcg
    29941 aagaagcaaa aaaataccat ttttattctg tctgcatcaa tccagcatgg gttgcgctag
    30001 cgaaagaaca attacaaggt gagccagtag ccgtatgtac agtaatcggg tttcctctag
    30061 gagcaaacac acccgagaca aaagcatttg aaacaacaga tgccatcaat aacggtgcag
    30121 atgaagtcga tatggtcatc aatatcggtg ctttgaaatc aaaggatgat caacaagtcc
    30181 aaaaagatat cgaagcagtt gttgaagcag caaaagatcg tgcgcttgtc aaagttatta
    30241 ttgaaacatc gttattagat agagaagaaa tcatccgagc ttgcgaaata gcaaaagctg
    30301 caggcgctga ttttgtcaaa acatcaactg gtttctcaac aggcggagca aaggtagagg
    30361 atgtccgttt gatgcgtgaa actgtaggac cagaaatggg tgtcaaagct tccggcggta
    30421 tccataacga agaagaagca atggcaatga tcgaagctgg tgctacaaga atcggaacaa
    30481 gcgcaggagt agcaatcgta tctggttcaa ctggtgaagg ctattaattt tttcaaagga
    30541 gaagctcatg gcagaactca aacaagaatg gatcaacaca gctattgaag cgttagataa
    30601 agcctatgtt ccttattccc attttccggt aggcgcttgt ctcgtaacgg aatcaggcaa
    30661 gatttatcaa ggcatcaaca ttgagaatgc atcatttgga ttaacgaatt gtgccgagcg
    30721 cacagctttt ttcaaagcag tatctgaagg agaaaaaaca tttacgcatc tagtcgtagc
    30781 ggggcacacc cccgatccta tctctccttg tggtgcatgt cgacaagtaa tggcagaatt
    30841 ctgttcccct gatatgccgg ttacgctagt aggagacaat ggtgtgacga aggcaacgac
    30901 cgttcgtgca ctgctgcctt atgcatttac tgaaaaagat ctataactca tgttcattca
    30961 gtcagctgac tgttgaaaaa taaaacaatt attttaggag gctttacaga aatgaaaaaa
    31021 gcaaaattat ttggtttagg tgctgtcgca ctagcagcag ggttattttt aggtgcatgc
    31081 ggcaacaacg gctctactga ttcatccggt ggcaaaagct catcagacac aacaactgca
    31141 gcattgatca cagacacagg tggtgtagac gaccgttcat tcaaccagtc agcttgggaa
    31201 ggactagaaa aatgggggaa agatcaagga ctttcacgag gaaatgatgg cttccaatat
    31261 ttccaatctt caaatgaatc agattacatc ccaaatattg accaagcatt gaatgctgga
    31321 ttcaaaacga tttttggtat tggttataaa ctaaaaccag cgatccaaga acaagcaaca
    31381 aacaatacag gtacgaactt tgttattatc gatgatgtca tcgatggatt agataacgtt
    31441 gtatctgcaa ctttcaaaga caacgaagct tcttatcttg caggtgttgc agcagcttat
    31501 acgactgaaa caaacgtagt cggctttatc ggcggtgtaa aaggtgaagt aatcgatcgt
    31561 tttgatgctg gattcaaagc cggtgttgat gcaggtgcga aagaattagg aaaagaaatc
    31621 aaagtattga atcaatatgc tggtgacttc tctgctccag ataaaggacg ttcaattgct
    31681 caaggtatgt atgcacaaaa cgcagatatc atcttccatg cttcaggcgg tacaggtaac
    31741 ggtgtattcc aagaagcaaa atcattgaac gaatcaggcg acaaaaaagt ttgggttatc
    31801 ggtgttgacc gcgaccaatc agacgaaggc gaatacacat taaacggcga aaagaaaaac
    31861 ttcacattga catctacact gaaagcagtt ggaacagtag tagaagattt ggcacaaaaa
    31921 tcagcggacg gtaaattccc tggcggcgaa cacactgttt atggattgaa agaagacggt
    31981 gttggtctaa cagaaggaca actttctgac gaagcgaaaa aagcagttga tgaagcaaaa
    32041 gaaaaaatca tttcaggtga cgtaaaagtt ccagaaactc ctgaagaaaa ctaaaaccat
    32101 ttctatagcc ttgttagaca catgtttgac aaggctatgg tttgattaga acaagaattg
    32161 ttcatttatc caagctgcct tgtgacagtt tggataaggg aacaactccc tgaagtaatg
    32221 tttgtttcaa aagaagaacg ctcactaagc gtgaatctta gtcaatcgtt gccctagata
    32281 gaaatcgtta ggatgtgaaa aaaaggtggt tcaagaaaat attgtcatag aaatgcagaa
    32341 tattaccaaa gaatttggaa cattcaaggc aaatgacaac atcaatttac aagtaaaggc
    32401 aggagaaatt catgcgttgc ttggagaaaa cggtgctggc aaatctacat tgatgaacgt
    32461 gctttccgga ttattagagc cgacatcagg gaaaattttg atgcgtggga aagaagtaaa
    32521 gatcacaagt ccgaccaaag ccaaccaatt agggattggg atggtccatc agcactttat
    32581 gcttgttgat gcctttactg taacagaaaa catcgtgttg ggaagcgagc ctagtcgcgc
    32641 agggatgctt gaccataaaa aagcacgaaa agagatccaa aaagtttctg aacagtatgg
    32701 attgtcagtc aacccagatg cttacgttcg tgatatttca gttggaatgg aacaacgggt
    32761 agaaatttta aaaacacttt accgaggagc agatgtactg atttttgatg agccgacagc
    32821 tgtattgacc cctcaagaaa ttgatgaatt aatcgtgatc atgaaggaat tagtcaaaga
    32881 aggcaagtcg atcattttga ttacgcataa gttagatgaa atcaaagcag tagctgaccg
    32941 ctgtacagtt atccgccgtg gaaaaggaat cggtacagtc aacgttaaag acgtaacctc
    33001 acaacaatta gccgatatga tggttggaag aacggtttca ttcaaaacga tgaaaaaaga
    33061 agcgaagcct caagaagtcg ttttgtctat tgaaaatcta gtggtgaaag aaaatcgtgg
    33121 attagaagcc gtgaaaaact tgaacttaga ggttcgtgct ggcgaagtac ttggtatcgc
    33181 tggaatcgat ggaaacgggc agtcggagtt gatccaagct ttgactggtt tgcgaaaggc
    33241 agaaagcgga catatcaagc taaaagggga agacatcact aataaaaaac cccgaaaaat
    33301 cactgaacat ggtgtaggac acgtgccaga agaccgtcat aaatacgggt tagtcctaga
    33361 tatgacatta tctgaaaata tcgcactaca aacctatcat caaaaacctt acagtaaaaa
    33421 cggcatgctg aattattcag taatgaatga acatgccaga gaattgatcg aagaatatga
    33481 tgttcgaaca acgaatgaac ttgttcccgc aaaagcttta tcaggcggaa atcagcaaaa
    33541 agcaattatc gctcgtgaag tcgaccgaga tcctgatctg ttgattgttg caaatccaac
    33601 tcgtggactg gatgtaggtg cgatcgaatt tattcataaa cgtctgatcg aacaaaggga
    33661 caaatacaaa gcagtgttac tgatcagttt cgaattagaa gaaattttaa atgtttcgga
    33721 tcgtattgct gttatccatg aaggagaaat tgtcggtatc gttgatccga aagaaacatc
    33781 tgaaaatgaa ttaggtctat tgatggcggg ctatacctta gaagaagcac gaaaagagtt
    33841 agaaaagggg gctggcgcac atgaatgatc gcagcgaaaa attccgtaat ttattagtgc
    33901 ctgtactttc agttgtttta ggattgatct taggcgcgat attgatggct gcttttggtt
    33961 ttcagcccat ccaaggttat tcttctatgc tagatgcagc ccttggttcg caaagaggga
    34021 taggtgaaac gttaaggcaa gcaacgccgt tgatttttac agcattaggt ttttctgtag
    34081 ctaactcagc tgggtttttc aatattgggc tttctggaca agcactttgc gggtggatcg
    34141 tcagtgtctg ggtggcactt gcgtttccag atctgccaaa agttgttttg cttccgttat
    34201 gcgtgatctt aggtgcctta gctggtgcga tggcagcggc tgttccaggt ttacttcgag
    34261 cattttttgg aacaagtgaa gtgatcgtaa caatcatgat gaactatatc ttattatacg
    34321 taagcacatt tgctttacat gaagtgatgc ctgaaagcta tcgctcaagt ttggactctt
    34381 ccaatatgat cagtcaaaat gcctcactgc ggttaccatg gttaacacaa gcttttggcg
    34441 gctctcgcgt aaactcagga ttatttttag caatcatcgc gcttattttc gtttggctgc
    34501 tgatgaagaa aacgacttta ggatttgaaa tccgttctgt tggattgaat ccttttgctt
    34561 ctgagtatgc aggaatgagt agtaaacgta cgatcgtttt gtcgatggtc atttctggcg
    34621 ctttggcagg gcttggcggt gtggttgaag gattaggaac gtatcaaaac ttctttgttc
    34681 aaacgacttc gctatctatt ggatttgacg gaatggctgt ttccttgctt ggttctggat
    34741 cagcaatcgg tattctttta tcagcgatct tgttcagtat tttgaagatc ggtggacttg
    34801 gcatgcagac aggagcaggt gttccgtttg agatcgtcaa tgtatcgatt gctttgatta
    34861 tcttctttgt cgggatcaac ttcctgattc gtttcgtcat ggctaaagtc ttccaaggga
    34921 aaaaacaaga agagatcgtt gcgacaatag aatcgaaacc aacgaatcaa aaccatgaag
    34981 ggggcgaact ataatgtcga ctatcgaatt aattgccttg atccttactt ctacactggt
    35041 ttattcaaca ccgctaattc taacgtcact aggcggaact ttttctgaac gaagcgggat
    35101 cgtaaacgta gggctagagg gaatcatggt catgggtgct tttagttcca tcgttttcaa
    35161 cttgacgatg agtccatcac ttggttctat gacaccatgg atcgctattt tagtgggtgg
    35221 cgtcgtaggt atcctatttt ctctgattca cgcagtagca acagtcagtt tgcgtgctga
    35281 ccatattatc agcgggacag taattaatct gatggcacct gctttaggtg ttttcttgat
    35341 caaagcatgg tatggtaaag gtcaaactga taacatcacg cagaatctgg ggtatttttc
    35401 tttcccagga ttagcagata ttccggtcat tggaccaatt ttctttaaaa atacattctt
    35461 acctgcttat tgtgctattt tgattgctgt cctagcttgg gtgatcatct tcaagacgaa
    35521 attcggcttg cgcctccgtt cagttggtga aaatcctcaa gctgccgaca cattaggtat
    35581 caatgtatat gctatgcgtt attcaggcgt actgatttct ggcttgttag gcggtatggg
    35641 cggtgctgtt tttgctcagt cgatttccgg taacttttca gctggtacga tcgttggaca
    35701 agggttcatc tccatggctg ctatgatctt tggtaagtgg aatcctttag gggcaatggg
    35761 tgcctcattg ttctttggtt ttgctcaaag cttgagtatc attggcgcac aattaccagt
    35821 tatttcttcg attccgccag tattgctgca aattgctcca tatttgctga caatcattgt
    35881 gctggttgtt ttcttaggga aagcttctgg tccaaaagca aacgggaaga actatatcaa
    35941 atcaaaataa tcttttagcg aataaaaagg gtgggttacg ggaagtgttt ttcgtctcgc
    36001 cctttttgtt ttaaaggcaa taaaaataaa aagaaagaag gaataatcat gtttaaacgc
    36061 gtacatctga tcgtcatgga ttcagtagga attggagaag caccagatgc cgataagttc
    36121 ggtgatgttg gttctgatac attaggacac attgcgaaag aagcaggatt gaacgttcca
    36181 catttagagc agcttggttt aggaacgatc cgtcctcttg aaggtgtgga atcagtgaaa
    36241 gatcatcaag gctatgcgac gaaattagaa gaagtttctg tagggaaaga tacgatgaca
    36301 ggccactggg aaatcatggg attgaatatc aaaactccat ttcgtgtgtt tcctaatgga
    36361 ttccctgatg atctgatcaa acaaatcgaa gaattttctg gccgtaaagt cgtatgtaac
    36421 aaaccttata gcggaacagc tgttattgac gattacggtg aacaccaaat gaaaacaggc
    36481 gatttaattg tatatacttc tgccgatcct gtccttcaga tcgcggcaca tgaagaaatc
    36541 attcctttag aagaattata tcggatttgc caattcgttc gtgatataac aaaagatgat
    36601 ccgtatatga ttggccgaat cattgcacgt ccgtacgtgg gtgaaccagg agacttcaca
    36661 cgaacaagca atcgccatga ttatgcgttg aatccattcg gacacacagt ccttgattct
    36721 ttgaaagaag caggaaagga cgtgattgct gttggaaaga tcaacgatat tttcaacgga
    36781 caaggaatca ctgaatctgt ccgcacgaaa agcaatatgg atggcgttga ccagctgctg
    36841 aaagttatga aaaacgattt ttcaggaatc agctttacca atttagtaga ttttgatgca
    36901 ttatacggtc atcgtcggga tgtagtcggc tatgcgcatg cgattgaaga atttgactta
    36961 cgaattccag aattgcttga agcgatggaa gaagacgact tgctaatgat tacagccgat
    37021 cacggaaacg atccgacgtt tactggtacc gatcatacgc gagaatatgt accgttattg
    37081 gtttacagta aaaaaatgaa agaacaaggg gcattgcctc aaggatatta ttcagatatt
    37141 tcagcaacga ttgctgagaa ctttagtgtt tcttcgactg aaaatggcga aagcttcttg
    37201 aaattattaa aataatcttt ttaaatagga gaaagcaaat gaccaagtta agcgagatgt
    37261 tacaagaaac gacccagttt atccttgata aaggagtaaa agaaattgat tttggtttga
    37321 ttctaggttc aggattaggt gaattagcag atgaaatcga agatgctgta atgatttctt
    37381 atgatgagat cccgttcttt cctgtttcga cagtagttgg gcatgcagga aagctagttt
    37441 atgggtctct ggcaggcaag aaagtgttag ccatgcaagg gagattccac tactatgaag
    37501 gtcattccat gcaaacagtg acctatccag tacgtgtgat ggccggacta ggggcccatt
    37561 cagtcattgt gactaatgca tgtggtggtg tcaacgaatc atttgtacca ggagatttga
    37621 tgctgattac cgatcatatc aattttactg gtgaaaatcc tttgatcggt ccgaatgaag
    37681 atgcaatggg ccctagattt ccagatatga gtcaagctta tacgcctgac tatcaagcaa
    37741 cagcaaaagc agcagcagaa aaactaggtt tggatcttaa agaaggcgta tatatgggct
    37801 attctggacc aacttatgaa acgcctgcgg agatccgaat gtctcggaca ataggagccg
    37861 atgcagtagg gatgtcgact gttccagaag tcatcgtagc agctcacagc ggattaaaag
    37921 tgttaggaat ctcttgtatt accaacttag cagcaggtat gcaagcgaat ctgagccatg
    37981 aagaagttgt agaaacaact cagagagtca aacaatcttt taaagcattg gttaaagaaa
    38041 cattgacatt attgtaatag gaggaacaat aaaatgagtg tgcatattga agcgaaagaa
    38101 ggcgagatcg ccgataagat tttacttccg ggagatcctt tgcgagctaa atatattgcg
    38161 gagacttttt tggaagatcc agtttgttac aatcaggtac gcggtatgtt aggctttacc
    38221 ggcaaatata aaggagaacg tatttctgtt caaggaacag gaatgggaat gccatcagcc
    38281 accatctatg cccatgaatt gatccagtcg tatggtgtaa agaaattgat tcgagtaggt
    38341 acatgcggtg cattgtcaaa agacgtccat gttcgtgatt tagtactagc acaaggtgca
    38401 gcgaccagtt cttcgatgat cgaaaaaaat ttccaagcct ttcattttcc tccaatcagt
    38461 gatttcaatc tattactgaa agcatatgag atagctaaag aaaagggcta tacggttcat
    38521 gtaggaaatg ttctttcgga agattctttc tacaaagatg atttgacaga gacgttccaa
    38581 ttagctgaac ttggcgtttt aggagtggaa atggaagcag cggctcttta ttacttaggc
    38641 gcgaaatacc atgtgcagac attaagtttg atgaccgtta gcgaccattt gatcaccggt
    38701 gaagaaacaa cagctgcaga acgtcaatct acgttcaatg aaatgatcga agtaggattg
    38761 gaaacagcga tagcagaata agaaggaaat gaaaaagctg ccctgcatta agtaataaga
    38821 acatccaagc aaaaatagct cctcatgttt ttgacttggc tggacttatt attgcaaatg
    38881 taagcttttg aacaccgttt accaagaggt tgtgaatctg ccgtttaact ctgagtcaca
    38941 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgaga
    39001 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
    39061 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
    39121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
    39181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
    39241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
    39301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
    39361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
    39421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
    39481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
    39541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
    39601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
    39661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
    39721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
    39781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
    39841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
    39901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
    39961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
    40021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
    40081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
    40141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
    40201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
    40261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
    40321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
    40381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
    40441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
    40501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
    40561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
    40621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
    40681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
    40741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
    40801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
    40861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
    40921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
    40981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
    41041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
    41101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
    41161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
    41221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
    41281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
    41341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
    41401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
    41461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
    41521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
    41581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
    41641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
    41701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
    41761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
    41821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
    41881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
    41941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
    42001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
    42061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
    42121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
    42181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
    42241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
    42301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
    42361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
    42421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
    42481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
    42541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
    42601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
    42661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
    42721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
    42781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
    42841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
    42901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
    42961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
    43021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
    43081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
    43141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
    43201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
    43261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
    43321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
    43381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
    43441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
    43501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
    43561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
    43621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
    43681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
    43741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
    43801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
    43861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
    43921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
    43981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
    44041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
    44101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
    44161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
    44221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
    44281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
    44341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
    44401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
    44461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
    44521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
    44581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
    44641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
    44701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
    44761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
    44821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
    44881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
    44941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
    45001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
    45061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
    45121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
    45181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
    45241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
    45301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
    45361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
    45421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
    45481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
    45541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
    45601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
    45661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
    45721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
    45781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
    45841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
    45901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
    45961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
    46021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
    46081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
    46141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
    46201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
    46261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
    46321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
    46381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
    46441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
    46501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
    46561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
    46621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
    46681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
    46741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
    46801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
    46861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
    46921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
    46981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
    47041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
    47101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
    47161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
    47221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
    47281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
    47341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
    47401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
    47461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
    47521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
    47581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
    47641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
    47701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
    47761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
    47821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
    47881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
    47941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
    48001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
    48061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
    48121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
    48181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
    48241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
    48301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
    48361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
    48421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
    48481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
    48541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
    48601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
    48661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
    48721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
    48781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
    48841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
    48901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
    48961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    49021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    49081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    49141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    49201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    49261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    49321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    49381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    49441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    49501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    49561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    49621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    49681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    49741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    49801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    49861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    49921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    49981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    50041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    50101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    50161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    50221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    50281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    50341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    50401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    50461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    50521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    50581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    50641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    50701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    50761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    50821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    50881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    50941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    51001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    51061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    51121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    51181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    51241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    51301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    51361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    51421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    51481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    51541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    51601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    51661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    51721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    51781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    51841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    51901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    51961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    52021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    52081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    52141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    52201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    52261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    52321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    52381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    52441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    52501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    52561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    52621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    52681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    52741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    52801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    52861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    52921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    52981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    53041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    53101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    53161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    53221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    53281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    53341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    53401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    53461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    53521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    53581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    53641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    53701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    53761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    53821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    53881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    53941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    54001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    54061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    54121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    54181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    54241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    54301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    54361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    54421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    54481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    54541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    54601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    54661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    54721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    54781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    54841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    54901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    54961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    55021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    55081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    55141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    55201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    55261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    55321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    55381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    55441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    55501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    55561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    55621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    55681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    55741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    55801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    55861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    55921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    55981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    56041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    56101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    56161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    56221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    56281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    56341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    56401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    56461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    56521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    56581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    56641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    56701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    56761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    56821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    56881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    56941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    57001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    57061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    57121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    57181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    57241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    57301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    57361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    57421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    57481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    57541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    57601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    57661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    57721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    57781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    57841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    57901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    57961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    58021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    58081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    58141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    58201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    58261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    58321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    58381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    58441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    58501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    58561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    58621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    58681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    58741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    58801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    58861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    58921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    58981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    59041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    59101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    59161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    59221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    59281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    59341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    59401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    59461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    59521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    59581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    59641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    59701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    59761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    59821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    59881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    59941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    60001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    60061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    60121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    60181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    60241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    60301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    60361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    60421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    60481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    60541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    60601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    60661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    60721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    60781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    60841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    60901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    60961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    61021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    61081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    61141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatga tttttcaaaa
    61201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    61261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    61321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    61381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    61441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    61501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    61561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    61621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    61681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    61741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    61801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    61861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    61921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    61981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    62041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    62101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    62161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    62221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    62281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    62341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    62401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    62461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    62521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    62581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    62641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    62701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    62761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    62821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    62881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    62941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    63001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    63061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    63121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    63181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    63241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    63301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    63361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    63421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    63481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    63541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    63601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    63661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    63721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    63781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    63841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    63901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    63961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    64021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    64081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    64141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    64201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    64261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    64321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    64381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    64441 attttcgaag aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt
    64501 aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg
    64561 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    64621 aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat
    64681 cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg
    64741 gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca
    64801 atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga
    64861 aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc
    64921 tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg
    64981 tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt
    65041 ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta
    65101 gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa
    65161 atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga
    65221 tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg
    65281 caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc
    65341 aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct
    65401 ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt
    65461 catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg
    65521 gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat
    65581 tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg
    65641 tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat
    65701 tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt
    65761 aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg taaccatagt
    65821 tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat
    65881 ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc
    65941 gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg
    66001 cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa agttttttat
    66061 gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg
    66121 aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga
    66181 gagcagctac gaccaaaatg attcctaaca acagcccacc atcgtcttgt tctatctttt
    66241 cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata
    66301 aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac
    66361 ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc
    66421 gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa
    66481 tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat
    66541 aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa
    66601 gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga
    66661 taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa
    66721 aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga
    66781 gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca
    66841 aaatcactaa tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag
    66901 tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct
    66961 tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca
    67021 gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga
    67081 tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg
    67141 tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa
    67201 actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac
    67261 ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc
    67321 cgttttctct aaagaatgac tttaaatttt ttttcatatt tattttccac caaatttcta
    67381 tgaagttgta ttcagtttat catatccttg tttttctctg cttaaaaaga gtctagtttt
    67441 cacaaaaaaa taaaatttag aataaataac ttcataattt gttcgtataa caaaaataaa
    67501 cgcaaaaaaa gggtaggaat caaaagtccc acccttaaaa atttttatta aataaaaaac
    67561 cgttcactat ctgaacggtc caagtatcat tcaattaaac gcgttcaact tttcctgatt
    67621 tcaaagcacg agttgacacc caaacttttt taggttttcc gtcgaccata acgcgaactt
    67681 tttgtaagtt aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc
    67741 cactttttgt cttgcgacct gtaaagtaac aaacttttgc catggtaatt tcctcctttg
    67801 ccttgatttg gagcaataga ataattcagc tcataccaga ctaacatatc atatagaatg
    67861 ggaatttgca agtacatttt catagataca attgacaatt gagaagaata attgtaaatt
    67921 aaacaaggtt atacctgaaa ttttgcaagg tttttgagta ctttgggtaa ctgcctttta
    67981 tttcaagttg cgctatgata aaatatgata gaagaaaaat gggagttttt tacccgttaa
    68041 ggaggctcat tcatggctgt aaaaattcaa acgtcagctg gaaccattga aattaccaat
    68101 gatgtaattg caacagttgt tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg
    68161 gccagcaaaa atcaaattaa agataatatt actgagattt tgcgtaaaga aaattactca
    68221 agaggagtag ttgtccgcca agaagataat ggtattgctg tagatgttta tacgattatc
    68281 agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat
    68341 ctagaggcat tattaggcgt aactgccaat tcagttaatg tctttgtaca aggtgttcgt
    68401 gtactgccag actaggcagg agactggctg gtaactcagc agtcgatctg aaggaggatt
    68461 atctaggtga gtgtaacaga aataagtgca agccagttcc aggaaatggt ccaggcaggt
    68521 gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt ccctgtgcca
    68581 gatggcgata cgggaacaaa tatgaatcta tctatgacta gtggagctaa ggcagtagta
    68641 gattcaacct cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg attgctgatg
    68701 ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt ttcgtggatt ttctaagcaa
    68761 attccagatg ttgtctcatt aaatgcttct gatcttgctg cagccttcac tcatggagtt
    68821 gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa cgattttgac agtagctaga
    68881 gaagcagcga aagctggaga aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg
    68941 acagctgttg taaaaggagc aaagcgtgct ttagctaaaa ctccagactt gcttccagtc
    69001 ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt ttatgagggc
    69061 tttttaagtg cattgaatgg agaattccaa gcggatgaaa catatgaacc ttctccagca
    69121 gaaatggatg atatggtcaa tgcagaacat catcgcagtg tccaaggtca aatggcgaca
    69181 gaagatatcc attttggtta ctgtacagaa atcatggtaa aaatcggaga aggacctaca
    69241 gtcgatagta cattcgatta cgaagaattc cgtaactatt tagatggtat aggagattct
    69301 ttacttgtgg tcaatgatga cgagatcatc aaagtccacg tgcatacaga aaacccagga
    69361 gaagtcttga attatggaca aaaattcggt tcattagtca aaatcaaagt cgacaacatg
    69421 cgcttgcagc atgagacgat tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa
    69481 gaaataccac gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag
    69541 ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg gacaaacaat gaatccaagt
    69601 acggaagaca ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca
    69661 aacaataaga acatcttcat ggcagctgat caagcagcag aagtagcaga tatcccagta
    69721 gcagttgttc cttcaagaac gatttcccaa ggattgacag ctatgctttc attcaatgat
    69781 caagcttctt tagaagaaaa caaagaaacg atgacagaaa tgttgtctag cgttgtgagc
    69841 ggtcaagtca ctcacgcgat cagagatacc tcaattgacg gtgtagagat caaagaagga
    69901 gactttttgg gcatgatcga tggaaaaatc ctgatctcta acccagatat cacagcaaca
    69961 gcgttaagta cactagaaca aatgatcaat gaggacacag agatcgtaac gatcttggca
    70021 ggtgatgaag gaacagttga acaagcaaat gaatttgctg agaaactaac agaaaaacat
    70081 cctgatttgg aaatcgagat ccaccaagga gatcagcctg tctatcctta tctattttct
    70141 gctgaataat ttctgtgtca gaatatcttt ctaacacagt attcattcat aaaaggcctt
    70201 cggttagtga tcaggtatga tcgctgacca agtccttttt ttgttaaagg agggaggaaa
    70261 aatttatgcg ttctttagat gatccaatca ctatgctggc aggagtcgga gaaaaaagag
    70321 cagctagtct tgccagcttg ggcatccaaa cgattgaaga cttactgact tattatcctt
    70381 ttcgctacga agatattcag gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta
    70441 ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc
    70501 gattgcaatt tcgtatgatg caagaacatg atgtgttcaa cgtttctttt tttaatcaac
    70561 cttatctaaa agaaaaagtc attttatcgg aggaaatcgc cgtatacggt aaatgggatg
    70621 caaagcgtaa agccttgaac gggatgaaga ttttaggttc tcaaacaatg gatgactttt
    70681 cgccgatata tcacgtcaac aaatcaattc gtcaaacgac attagttgat ctaatcaaaa
    70741 aagggtttca agaatacggc acattgattg aagaaaatct gccaaacaac cttgtggaga
    70801 aatatcgact gcttgaccga gcttctgctg taagaagtat gcattttcca aaagaccacg
    70861 aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc
    70921 gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag atcctttatg
    70981 atgtccaaaa attgaaacaa tttactcaaa agctaccttt tgaactgaca aatgcacaaa
    71041 aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt
    71101 tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta tacgctaccg
    71161 ttacagcagg tttccaaggt gcgttgatgg tgcctacaga aatcttggca caacaacata
    71221 tggaaagctt gcaacagctg tttgatccca atgaagtaac catcgcattg attacggggt
    71281 caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg
    71341 tcgtgatcgg cacacatgca ttgatccaag atggagtcga atttcagaat ttaggtctag
    71401 ttattacgga tgagcagcac cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag
    71461 gctggcgacc agatgtctta tttatgacag cgactccgat tcctagaaca ttagctatta
    71521 cagcatatgg tgagatggat gtatctatca ttgatgaact tccagctgga cgtatcccga
    71581 tcgaaacacg ctgggtaaga acacctcaat tggacagtgt actggattgg acgaaaaaag
    71641 aactggcaag aggacatcaa atgtatgtca tctgtccgct gattgaagaa tcagaagcat
    71701 tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg tgaactattt gcaccaaaat
    71761 atgaagttgg cttgttacat ggtaaaatga agaatcagga aaaagaagta atcatggaag
    71821 ccttcaaaga caaccagatg cagatattag tttcgactac tgtaattgaa gtaggggtaa
    71881 acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga tcgatttgga ttggcacagc
    71941 tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt atcttagttg
    72001 ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat catgacagag accaccaatg
    72061 gttttgtact aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta tttggcttcc
    72121 gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt gacggatgga aatgttttgg
    72181 aggtagccag agaagaggct gcagcccttt ggcaaataaa ggattggcag atgcttcctg
    72241 agtatcagcc tttagcgcaa agcctccaaa tagaaaatca ggataatcgt tatttcgatt
    72301 agcagactgt gctatacttt atactgatga cggaaaaagg agtggaaaca atgaaaattg
    72361 ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc gtggctttag
    72421 cgcagcaaga ttttccagaa gtagaattct tactttacgg aaaagaagca gaaatcaaga
    72481 aatacttaac aaatgaaaaa aacatcacga ttatccatac agacgaaaaa atcaatagtg
    72541 atgatgagcc agtaaaagca attcgacgaa aaaagactgc atcaatggtc cttgctgcac
    72601 aagcagtgaa aaacggtgaa gcagatgctg tattttcagc agggaataca ggcgcgctac
    72661 tagcagcagg gttgttcatc gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt
    72721 ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga tatgctggat cttggcgcta
    72781 acgcagaaaa caaaccagaa catttgttgc aatacggtat tttaggttct ttttatgcac
    72841 gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa
    72901 caaaaggtag tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa acaagcttga
    72961 attttattgg aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat gtagtggtca
    73021 cagatggatt tacaggcaac gctgttttga aatctattga aggaacagca atgaatatga
    73081 tgaatctatt aaaatctgct attctaaatg aagggatcaa aggaaaaatg ggcgctatgc
    73141 ttttaaaaga cggcttacgt tctttgaaag cagaaatgga ttattccaaa catggcggtg
    73201 ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca tggagcaaca ggccccgatg
    73261 ctgttcgcta tacgatccgc caaatccaca caatgcttga gacagatgta gttggacaat
    73321 tagtcgaaca atttgaaaaa agcgaagaat agtatttgga tatctgaagc gatccgttcc
    73381 attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa
    73441 ttgacaagtg tatgattctt ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa
    73501 gtagcgaaaa ttatttcaaa tcattttgag atagataccg ataaagtgac ggatgaattg
    73561 aacatcaaag atgatttaaa agctgattca attagtatta tggaattcgt tttagagctt
    73621 gaagatgaat tcggtacgga aatttcagat gaagacgctg aacaaattga aacagtcggt
    73681 ggcgcagtag attatatcag cagccatctt aaataatttc aaataaaagc aaagaaggat
    73741 aaaaagctgc atgcagcttt ttatccttct ttttttaatc tttttgacac gtaaaagtca
    73801 tttttctgat aattctggcg ataaacagaa attatgttgc aaagtaaagc ggttttctat
    73861 ataattatta ataattcatt acacaaagat aagaataacc tttgttataa aacgatttat
    73921 tctgtgtaat gtcctttttt attaagaaaa acttccattt aggaagcaga gaggggtatt
    73981 cgattgtccg ataaccaatt attagaagtt cagaacctac atacaggatt ccgactcaaa
    74041 gatgactatt acgatgcagt agatgacgtt tcatttactt tagacaaaaa cgagatttta
    74101 gcaatcgttg gggaatctgg gtgcgggaaa agtaccttgg ctactacgat tatgggatta
    74161 cttgatccta ataacacaaa aattacaggt gagattttat ataacgatct gaatttaatc
    74221 gacctgaacg aaacgctcta taataaaatc cgcggaaatg atatcggaat gattttccaa
    74281 gatccgttgt ctgcattaaa tcctctcatg agaatcgaag accagatcaa agaaggactt
    74341 tcctatcaca caaagatgaa tgcagaacag cgtcaagctc gtgcactaga attgctagaa
    74401 caagtaggga ttcctaatcc tgcgagagtt ggacgccaat atccccatga actatcaggc
    74461 ggtatgagac aacgtgtcat catcgcaatc gcaatcgcct gcaaacctcc aatcgtcatt
    74521 gcagatgaac cgacgacagc cctagatgta accatccaag cacagattct tgatttactg
    74581 aaagaccttc aggaagaaac aaaaacaggg atcatcttga tcactcatga cttaggagtc
    74641 gtagcagaaa tggctgatcg agtggcagtg atgtatggcg gacagttcgt tgaagtagca
    74701 ggtgtaaaag aactgtttga tcatcctaag catccttata cacgctcttt actgaactct
    74761 ataccacaag aaggaaatca tgatgcagaa ctccatgtga tcgaaggtgt cgttccttct
    74821 ctgaaaaaca tgcctcgtaa aggttgtcgg tttgcaccac gtattccatg gataccagca
    74881 agcgatcatg aagaagagcc tatccttcat gaagtcgcac cgaaccactt ggtacgttgc
    74941 tcctgctata agcatttcca ctttagagac gaaaaaggag aggtgtagat gacagaatta
    75001 atcacaatca aagacctgaa agttcattac cctattcgca gtggattctt caatcgagta
    75061 acggatcatg tatatgctgt agatggtgtg gattttatta tcgaaaaagg aaagacatat
    75121 ggtttagtgg gagagtctgg ctctggaaaa tctactacgg gaaaagccgt tgtgggatta
    75181 gaaaaggtaa cagccggaca aatcatgtat gaaggaaaag atgtaacgaa gcgaagcaac
    75241 cgcaaaaaaa tgggttataa caaggatgtg cagatgatct tccaagattc aatgtccagt
    75301 ttgaatccta aaaagcgagt gttggatatc attgctgaac ccatccgaaa ttttgaacgg
    75361 ttaagcgatc aagaagaaaa gaaaaaggta aaaggactgc tagatatcgt tgggatgccg
    75421 gaagatgcgc tatataaata tcctcatgaa ttttcaggcg gacaacgtca acgtctagga
    75481 gtagcccgtg ctgttgctac caatccaaaa ctgatcgtag ccgatgaacc tgtatctgct
    75541 ctggatctat ctgttcaagc gcaagtattg aatttcatga aacgaatcca gcaagaatac
    75601 ggcctcagct atttattcat ttcacatgat ttaggtgtag taaaacacat gtgcgacaat
    75661 atcgcaatta tgtacaaagg tcgatttgta gaaatcggta caagagaaga catttataat
    75721 gacccgcgac atatctatac gaaacgtctg ctttcagcta ttccgcaaat cgatgtagaa
    75781 aatagggaaa gccacaaaga aaatcgacga agagtagaac aagagtatat ccaaaaccaa
    75841 aaagaatact atgatgcgac tggtcgagtc tacgatttgc gtacgatcac aactacacac
    75901 aaagtggcgt tgaaagatgg aggtgcaagc taatgtggaa aacgattctg cgacgtgttt
    75961 tattgatgat cccgcaagtt attattttaa gtgttctgat cttcttatta gcccaagcga
    76021 tgccggggga tccatttacc ggtttgatca atccaaatca ggatccggca gtgatcgaac
    76081 agatgcgaca agcagctggc ttgaatgacc catggtatgt ccagtacttc cgttggatcg
    76141 gaaatgcttt gcaaggcgat tttggtcaaa gtttcttata caaacgctct gttgctgact
    76201 tgatcggcga acggatcatc aacactttat atctatcgat cttgacagtt gtccttactt
    76261 atttgattgc tgtgcctctg gggatgcttg ctggccggta tcaaaattca atcttggata
    76321 agattgtcgt aatctacaac ttctttagtt ttgctgttcc tttatttatc ttcggcttga
    76381 tcatgctatt tgtttttggt tatcgtttag gctggttccc aacaagtgga tcacaaacac
    76441 taggtttgca aggtggattt atcactcaat ggatagaccg tttgaaatat attctgttac
    76501 catcaattac ccaagcattt ttggcgactg ccgtcacaat ccaatatctg cgaagtgaag
    76561 taatcgattc gaaatcgctc gattttgtca gaacagcccg ttccaaaggg gtaccgacga
    76621 ataaagtatt cagtcgacat attttccgaa atgcagcgtt gccgatggct tctcaaatgg
    76681 ggtatgaaat cacttcgttg attgctggtt ctgtagttat cgaaaagatt tttggttatc
    76741 cgggagtcgg aaaactattt attgattcca tcggccaacg ggactatacc gtgatcacat
    76801 ccttagtatt gattttagga atcgcgacgc tagtggggac attactttca gatatcatca
    76861 tgagtatcgt tgacccgcgt attcggattc aataaaacca atcagaaagg agaatggatc
    76921 gatgagtgaa aaaaacaata cagcagttca agaaagtatt cctcctatgg ggatacgaat
    76981 gattgctcga gaatttaaaa aagataaaat tgctattttt tcattaggtt tgctagtggt
    77041 tttattatta gtgatcttta tcggtgcgat gctgaccgat caagacaaag tgatgtatgt
    77101 tagtatcttc gacaaatacg ctgcaccagg aacagtttcg acacaaggac aaaagttcat
    77161 tctgggagca gatgaaggcg gacgtgatgt attaggacaa ttgatcatag gtgctagaaa
    77221 ctctgttacg atcggatttg cgatcacgat ccttacttcg atcatcgggg ttggattagg
    77281 gatcatctct ggctactacg gagggatcat tgacaacatc ttgatgcgga tcgttgattt
    77341 tatcatgatt ctaccgatca tgctgatcat tatcgtcttc gtatcgatta tttccagata
    77401 caatgtctgg tcctttatat tcatcatgag tgcattttat tgggtagcta aagcccgatt
    77461 attccgaagc aagacattat ctgaagcaag aagagattat gtcagtgcct caaaaacaat
    77521 gggaaccaat gattttaaaa tcatgttccg cgaaatcatg ccgaatctaa gttcattaat
    77581 cattactaat ttgacgatga actttgcggc aaacatcggt atcgagacaa ctttgacttt
    77641 cttaggtttc ggcttgccgg caaatgtacc tagtttaggt acattgatcg gttatgcaag
    77701 taacggagaa gtcttagtga atcggcaatg gatctggtta cccgcatcaa tccttatttt
    77761 agttttgatg ttgagtatca attatgttgg acaagcattc aagcgttctg cagatgcgcg
    77821 ccaacggtta ggctagtata gaagcgaagg gggaaatagg aatgaagaaa acgatttttg
    77881 gatttgcagc gctgatgtct gctgtcgttc ttgcagcatg cgggggcgga ggtacagata
    77941 cctctggaaa caactctgga ggcaacggca ataacaaaga ggaattaact tttccaattg
    78001 caacaaaaaa taatgatgat gcaatcgaag acggcgaatt agaagctgcg gtcgcaacgg
    78061 acagtcagtt taaaggattg ttccagtggg agttttacca agattcatat gatgcagctt
    78121 ttatggctcc ttctcatgaa ccgctatttg caaacgatga cgatttcaga atcacagacg
    78181 acggagctgc aacgcttgat ctagatgaag atacgaaaaa agcgacaatc aaagtaaaag
    78241 acaacgtcaa atggtcagat ggtgaagatg taacggcgga cgatgtgatt ttcccttacg
    78301 aaatcattgg gaataaagat tacactggta tccgttatga cgacactttc cgtaacatcg
    78361 taggtatgga agaatatcat gatggaagtg ccgacacaat ctccggaatc aaaaaaatag
    78421 acgacaaaac agttgaaatc gaatataaag aagtaaaccc aagcatgctt caatcgggtg
    78481 gtggtatctg gtcatatgca gcaccaaaac atacgttaga aggtgtagca atcaaagata
    78541 tggaatcaag cgatcaagta cgtaaaaatc cagtgacttt tggcccttat tacatgagta
    78601 atatcgttgc aggagaatca gtagaatact tgccaaacga gcactactgg aatggtacgc
    78661 ctaaattgaa aaagatcacg atgaaatctg taccgatggc aagtgcaact gaagcattga
    78721 actctaaatt atatgatatg attttccaaa tgccaacaga tacttacgat acctataaag
    78781 aaattgctgg ctatacaaac ttaggccgtg aacaaacttc ttatacttat ctaggattca
    78841 agttaggtaa atggaatgca gatacaaaga gtgtggaata caatccagat tcaaaaatgg
    78901 ctgataaatc attacgtcaa gcaatgggct atgcattaga taataatgca gtaggtgaac
    78961 gtttctatgc aggtttgaga agcaatgcga cttcattgat tcctccagta ttccagtcat
    79021 accatgatga tgagatgaaa ggattcacac aagatctaga caaagcgaaa aaactattag
    79081 atgatgctgg ctacaaagat gtggatgacg atggtttaag agaagacaaa gatggaaata
    79141 aactaacaat caactttgct tcgatgtccg gtggggaaac agcgcaacca ttggcagatt
    79201 actatatcca acaatggaaa gaaattggtt tggatgtcca atacacaaca ggtcgtctga
    79261 tcgaattcaa ttctttctat gataaattag aaaacgatga tcctgaaatt gatgtttacc
    79321 aagctgcttg gaatacagga actgatccaa atccaagtgg actatgggga ccaaatgctg
    79381 cgttcaacta cactcgttat gaatcagaag aaaatacaaa attgatcaac gatatcagtt
    79441 caagcaaagc atttgatgaa gagtaccaaa aagaagcata tcaaaaatgg caacaatatg
    79501 catttgaaga agcatttgcg attccaacgt tgttccgtaa tgaagtattg ccagtcaacg
    79561 accgtgtcaa agactgggat tggtcttatg atgtaccaaa cccatgggca aatgtttctg
    79621 ttacagcaga ttcacgttca taatcactaa aaatgaccga aaaagggcga tgtccttttt
    79681 cggttttttg atatttttct gtttatttct ctaatattca ggtcaaggcg ctgatttcat
    79741 gacttctcca ataaacggtg ttcaaagtcc agtttctcgg tattaagtca aatttatgca
    79801 aaaatatgga aagccatttt cacaaaattt tttcttcata ctcgaaacta aacgaatttt
    79861 tcacaacttc tcgtcaatca ctaggattat taaagcttta cttacctttt gaccgaacca
    79921 tttcaaattt gacaaaaaac cagtataata aggaagtatc gaaaaaatat ccataaaagg
    79981 aatgtggaaa atggacaacc agctgacaaa agaactaaaa gaaaactata acatcgtttt
    80041 tcacgatcta aatttgcttg aacaagcttt tacccattca tcctatgtga atgagcatcg
    80101 caatctgcaa ttatcggata atgagcgact tgaattttta ggagatgccg ttttagaact
    80161 gatggtttct cagtatttat tccgtttata tcctcatttg ccagaaggaa aattgacaaa
    80221 aacacgagct gcgatcgttc gcgaagacag cttgtcgaaa tttgccaaag aatgtcactt
    80281 tgatcaatat gttttgttgg gcaagggaga agagaattca ggaggacgta cgcgaccagc
    80341 attgctttgc gacctattcg aagctttttt aggcgcgtta tatcttgacc agggattcga
    80401 cacgacccat gcattcatcg aaaaagtgat ttttcctaaa gtcaaagcag gtgctttttc
    80461 acatgagatg gatcataaaa caaaactaca agaagtcttg caaaaatcag gtgatgtatc
    80521 aatcgaatac cgattgatca atgaagaagg tccagctcat gaacgagtat tttggatcga
    80581 agtttatgta gatgatcaat tgattggcac tggacaaggg aaatcgaaaa aattagcaga
    80641 acaagctgcg gcggaaaatg cattggcagc actgtaaaag taagtgaggg aaagctttgt
    80701 atttgaaacg aattgaaata gccggtttta aatcatttgc agacaagaca gtcattgatt
    80761 ttgaaaatag cgtcacagca gtggttggac caaatggcag cggaaaaagc aatattacag
    80821 aggctatccg ctgggtatta ggagaacaat ctgctaaaag tctgcgaggc ggaaaaatgc
    80881 cggatatcat ctttgccggt tccgataccc gtaagcagct gaatattgca gaagtcacag
    80941 tgattttaga caatagtgat cattatctgc cattggagta caatgagatc agtgtaaccc
    81001 gccgttatcg acggactggg gaaagcgagt ttttcatcaa taaacaaagc tgcagattaa
    81061 aagatatcca agaattgttc ttagattctg gattaggaaa agaatcgttc tcaatcattt
    81121 ctcaaggaaa agtagaagcg attttcagta gtaaaccgga agaccgtcgt gggatctttg
    81181 aagaagcagc tggtgtcttg aaatacaagc agcggaaaaa gaaagcagaa cagaagcttt
    81241 ttgaaacaga agacaacctc agccgcgtcc aagacatcat ccacgaattg gaagaacagt
    81301 taactccttt ggccgcccag agtgaggcag caaaggagtt tttacggcta aaagaaacac
    81361 tgactcaaac cgatgtctct ttgatggttg ctgaaatcaa gacagcaaaa aaagattggg
    81421 acaacaaaca agcacagctt gcgaaattca atctagaatt aggcaaacta tctgagtcga
    81481 tccaagaaca agaatccatt ttggcgaagc agcgtaaaga aaatgcgcaa gccgatcgtc
    81541 tgatcgaaaa aaatcagcaa gtattattag atctttcgga aaaactgaag cagacagaag
    81601 gacaaaaaga tgttttgcag gagcggacaa agcatacaca aaaaagctcg caagaatatc
    81661 aaacttcatt agcagaagca caaaaaaagg ttaaacattt tgaaaagctt caagaaagct
    81721 tgatgaaagc agcagcagaa aaagaaacag agatccaaaa agctgaagca aatctcataa
    81781 aaacacagca agaattagaa aaataccaaa aatcaacgaa agaattattg gcagaattaa
    81841 gagatcaata tgttgatttg atgcaagaac aagcagctgt aggaaacgaa ctgaagtatc
    81901 tagaacgtca atatatacaa gaaacagcaa aaagcaagca gacgttagcc aagcaatcgg
    81961 aagtagaagc atcagtagac cgattgatct tacaaaaaga agaattgaca caaaagcagg
    82021 cgcagctaaa atcatcttta actgaaacaa aagagaagtt agaaatgatc caacaaaacg
    82081 ggaaaaagtt ccaagaagca ttagcaaaag aacagcctaa aatgtatcaa ttaatgaatc
    82141 aagtccagca gctacgtgct cgtaagaaaa gccttcaaga aatccaagaa aactattttg
    82201 gtttttacca aggggttcga ttggtcttgc agcataaaca gcagctttcg ggtattgtcg
    82261 gagcagtggc tgaactgatc gatgttcctg cagatttcac gttagctatc gaaacagctt
    82321 taggtggggc agcccagcat gtcatcgttg aaaatgaaaa agatgctcgg caagccatca
    82381 cgtatttgaa acagcaaaga ggaggaagag caacattcct tccactgacg acaatcaaac
    82441 caagacaatt accggctcat atattgacac aagcagcagc tgtagaaggg tttataggga
    82501 ttgccagtga acaagtcagt tatcccgatc aaatccaaac tgtggttcaa aacctgctag
    82561 gaacgatcct tttagccaaa gacttaacta gtgccaatgc aatcgcgcaa acgatccgct
    82621 atcaatatcg agtggtttcc ttagaaggtg atgtgatgaa tgccggagga tccatgacag
    82681 gtggagcgaa taaacgagga aaccaaggca gtttgtttgt acaaaatcaa gaattgaaac
    82741 agttgactag tgaatttgaa gaagcagata aacaactcca agcacaagaa aaaaaagttc
    82801 aggagctgca acaagaaaca gctcgtttag ccgaagaaca agaagttttg cgtacacgag
    82861 gcgaacaatt acgttttgaa gaacaagaag cgacaaatca gctacaaaac atcataaatg
    82921 aacttgaacg ttttgaaaaa gaaaagcaaa tctcaacttt tgagacacgt gaactgcagc
    82981 agtttatcga agattacgaa aagcaaacga acgaattgaa agacaaacaa acagatcttg
    83041 aatcgcaacg acaaaaaatc gacgaagaaa tcaaatcgtt aagtcaagaa agcgaccaga
    83101 tggaagccag aagagcacaa gtacaaagtc aaaaagcaca agaacaagca gatcttgctg
    83161 tcttgaaaga gcagtttaat catctgcaga tccagctccg cggtgcccga gtccaaaaag
    83221 cagaggcaac cgaacgtcaa gaagcgatag aaaagcagtt ggcgacattg acagcagact
    83281 tttctgatca tgaagtcaca gaagaaagtt tggaaaaaca aattaacgaa ttatctgctc
    83341 agcgagaaac attaaaagcc gagttggcaa aagccaaaga gcagcgagat agaaggcaaa
    83401 aggagatcga taagcttgaa gcagtcttgg cagagcgtaa tcgtgagcaa aaagcacgac
    83461 tgtcggaaca atccaaatta gaagtccaaa aagaccgagc tgaaatgatt ttagataatc
    83521 atttgaccta tctgcagtca gaatatcagc tgacatttga gaaagcttcc caagactatc
    83581 aagaaacaac cgatatcgaa gacagtagaa caaaggtttc atccttaaaa gaacagattg
    83641 aaaaactggg accagtaaac ttaaattcaa tcgaacaata cgaacaagtc agtgaaagac
    83701 atacattctt agcaacacag cgagatgatc tgttagcagc gaaaaaccaa ttgtttgaaa
    83761 cgatggatga gatggatgat gaagtgcgga caagattcaa agaagtattt gaagctatcc
    83821 gtcaagaatt caaaattgtc tttccaaata tgtttggcgg cggtaaagct gaattagtac
    83881 tgacggaacc aactgatttg ctgaaaacag gtattgagat tgaagtacaa cctcctggca
    83941 aaaaactgca aagtctaagt ttgctttctg gaggagaacg agcacttaca gccatcgcgt
    84001 tgctcttttc cattatccgt gtctgtcctg ttccgttttg tatcttagat gaggtagaag
    84061 cagcgttaga tgaagccaac gtgaaacgtt ttggtcgtta cttgagcgat ttccaagatg
    84121 acacacaatt tatcgtagtc acacaccgca aaggaacgat ggtcgcagca gatgtactgt
    84181 acggagtgac gatgcaggaa tccggtgtat caaaaatcgt ttctgtacgg atggaagata
    84241 tcaatgaaga ggcgaaaatc aacgaaacga aagtgtagga acgaaaatga tcaaattagt
    84301 agcaatcgat ttggacggta cattattaaa tgaacaaaag ctgatttcag atgagaataa
    84361 acaagcatta gcacaagcaa aacagcaagg ggtcaagatc gttctatgta caggacgccc
    84421 cttagctgcg atggcgcatt atctgcaaga attaggactg gtagatgaag gtgactacag
    84481 catcactttc aacggcggtt tggtacaaaa aaatgatact ggtgaaatca tggaaaagaa
    84541 agtgatggaa gtatcagata tccaacgctt atacaaactg gctcagcaat tggatctgcc
    84601 actagatgtt ttgtctgaca atgtggtttt acagctgcct agtgccccaa ggaagcaatc
    84661 cttgtacaat gtgttgaata agctgctgca attccaacca gcggatataa gcgatattac
    84721 agatgaattt gtgcttaata aggcagtaat cgcttatgac caagaagaat tagatcctaa
    84781 aatcaaagaa ataccagcag aattccatga aatgtatgag atcatcaaaa caagaagtat
    84841 gcttttggaa tttatgccaa aaggtgttac aaaagcttat ggtatttctt tattggcaaa
    84901 agatcttggt ttgaaacaag aagaaatcat ggcgatcggt gatgaggaaa atgatttacc
    84961 gatgatccaa tatgctggac taggcgtagc gatggagaat gctgtgcctt tcgtcaaaga
    85021 agcagcagat ttcgtgacag ccagcaatga agaaaatggc gtagccaaag tgatccaaaa
    85081 atttgtatta gagtaggggg agtcaaatgg gattttttga taaaatcaag cgtgctttta
    85141 ctggcgaaga gaaaacagaa gaagttgaag tacaggagaa gtacgacaag ggtttagaaa
    85201 agacgcgtaa aacatttggt gaacggatga atgagttgtt tgccaatttt cgaacagtcg
    85261 atgaagactt ttttgaagaa ttagaagaaa cacttatcgg tgcagatgta ggttttgaaa
    85321 cagcgatacg aattaccgaa gcattacgtc aagaagtgaa actcagaaat gcgaaaaaat
    85381 cggcagaagt ccaaaatgcg atcatcgaga aacttgtgga tctctatgaa gcggaaggaa
    85441 tcaatgaagt aaatgaacta aatatccaag aagacggact aacagttatt ttgtttgtcg
    85501 gtgttaatgg tgtagggaaa acaacaagta ttggtaaact tgcccatcag ttcaaaaacg
    85561 aaggtaaaaa agtattgatg gcagcagcag ataccttccg cgctggagca atcgaccagt
    85621 tagttgtctg gggcgaacgc gctggtgttg aagttgttcg tggcaatgca ggaggcgatc
    85681 cagcagctgt cgtattcgat gctttggaaa gagcaaagac agagcaggca gatgttttac
    85741 tagttgatac tgcaggccga ctgcaaaata aagtgaactt gatgaacgaa ttagaaaaaa
    85801 taaaacgagt aatccagcgg gaattaccag aagcacccca agaaacactt cttgtattag
    85861 atgcaaccac tggtcaaaac gcgatggttc aagcaaaaca attcaaagaa acaacagatg
    85921 tgaccggtct tgttttgaca aaattagatg gtacagcaaa gggcggtatc gtacttgcta
    85981 tccgtaatga attgcattta ccagtgaaat tggtcggtct tggtgaagga atcgatgact
    86041 tggagccgtt tgatccaaat gattttgtcg ttggattgtt caaaggatta ctaaaagaag
    86101 aataacccat gtctaaaaag caggagctgt gacaaaagtt gcagctcctt gtttgaatga
    86161 ttgtttttaa catagctgtg tttttgatgc cttcattttt atacttattt gtaagcggtt
    86221 ctcaaacaat ttcgttcttt ttcttagact ttttgattat aattagattc atatgaattg
    86281 gaggtcactt aatgatgaat tttaagcaat tattattgca tgtcaatgcg cgccctttta
    86341 tcgaccaagc tcgatttggt atagaacggg aaggacagcg ggttgatctt gcaggaaatc
    86401 tagcaaaaac cgatcatcca gcaatctttg gcgatcgatc ctatcacccc tatatccaaa
    86461 cagattttag cgaaacacaa acagagatga tcacccctgt taccgattct attcccgaat
    86521 tatttcagta tctcgctgct gtttatgatg tgactgctcg ttctataccg aaagaagaga
    86581 tgatctggcc attaagtatg ccacctgcct taccggaaaa agacgaagag atcattattg
    86641 caaaattaaa aaatttcgaa gatgtcttgt atcgacgtta tttagcaaaa gaatacggga
    86701 agcgaaaaca gatggtaagc ggtattcatt tcaattttga atttggcgat gagttgctaa
    86761 gaacattgtt cagccatcag gaagaatttc aagatttttc cgaattcaaa acagaacttt
    86821 atttgaaaac agcgagaaac tttatgcgct atcgctggat gatcacttat ttattcggtg
    86881 cttcaccaat gagtgagaag aactactttt tagatgaatc acatccgcaa gaacctgttc
    86941 gcagtattcg caacagtgcg ctgggctata cgaatcatcc aaatgtgaaa gtttcctatg
    87001 cttctatgaa acagtattta gcagatatcg agcgaatgat cgaagaaggg aaactttcgg
    87061 aggagaaaga attttataca ccgcttcgtt tccgaggcgg aaagaaagtc gcagatttag
    87121 caacaacagg tgttcgttat attgaattgc ggaatatcga tttgaatcct tatgcaagat
    87181 tgggtatcaa tccagagcaa gttcggttct tacaattatt cctgatgtac atgttatgga
    87241 cagaagaaaa agaagactgt gaccaatggg tagcagaagg tacgactcga aataacaaag
    87301 tagcactaga acaaccatca gatcaaacag aatttcatca agaaggtaga gaaattcttg
    87361 aaggaatgaa gcaaatgctg gttgaattgg attggttgga ttctctttat ttagtagagg
    87421 aagcattgac tcaaatggat catcctgaac aaacgttagc agcgaaactc tatcaagaag
    87481 cacagctatc gagtcagcaa gaagttgctg tggcattggg acatcaatat tataaagaaa
    87541 gccatgaacg tccctatcag ttggccggtt ttcgcgaaat ggaactttct actcagatat
    87601 ttatgttcga tgccatccaa aaaggtgtcc aagtcaaagt attagatgag tcggatcaat
    87661 ttttgcgtct gcaattccaa gaccatgtag aatatgtgaa aaatgcaaac atgacaagta
    87721 aggacagcta tatcgtccca cttatcatgg aaaacaaaac agtaacgaaa aaagtcttaa
    87781 aagaagctgg gttccgggta ccaggaggcg cagaattttc atccatggaa gaagcggtaa
    87841 aagcttatcc gagatttgcc gagcaggcat ttgtgatcaa acctaaatca accaattacg
    87901 ggttaggtat cacgattttc aaggaaggag ctagtctgga agattatcaa gcaggtttag
    87961 ccattgcttt tcgtgaagac agttcggttt tagtagaaga atttatgccg ggaacggaat
    88021 atcgtttctt tgtgatcgac ggagaagtcc aagccatcat gttgcgagtc cctgctaatg
    88081 ttattggtga cagtatccga acagtgaaag aacttgttga agaaaaaaat agtgaccctt
    88141 tgcgtggaac gaatcatcgt gcacctttag aattgatcca attaggtgaa ttagaacaat
    88201 tgatgttgaa agaacaaggg ttgacgatcg aatcagttcc ccaagccaat caaattgttt
    88261 atctaaggga gaactcaaat atcagtacag gtggtgattc gatcgatatg actgatgaat
    88321 tttctgaagc ttacaaaaaa atcgccgttt ctgcggtaga agcactagga gctaagatta
    88381 gtggtatcga tttgattata ccagataaag aaatcgatcc tacaactgat aaaaaagcat
    88441 atgggatcat cgaagcaaat ttcaatccag ccatgcatat gcatgtttat ccttttgcag
    88501 gaaaaggcag aagattgacg atgaatgtat tgaaattgtt atatcctgaa gtattttaaa
    88561 agaaaaagct tttgacagat tgtcctattt gatgtcaatc tgatcaaaag ctttttttgc
    88621 atgtaaaaat ttatgtgttt tttcgtttta aaataaacga tcccagaagc tttttttctt
    88681 ttttggtttt tctttttcag ctggcgaaga gttttcttct gggtattttt tttcgatatc
    88741 aatcacaggt tcatttactg cttcttttgc tgttaagagc aatccgaatt cctcaggatt
    88801 tgcagtgacg taatcgttga ctacagtgaa acggctattg tttttcgttg ctagctggat
    88861 atattggttt tggacagaaa ggggtagagc cccattcaag agtatggaag catctggatg
    88921 ttttttgagt tcttttaaaa agttttcttt gttttttggt tggatcattt cagcaacagt
    88981 catactaaga tagcagcgtt ccctgaatgt ccctaaatat ttatgctgtt cttcagggtt
    89041 cactaaaggt gtaccataaa gtcccttatc caaatggctt tttacatcat cttgtgccat
    89101 aaaaaatacc ctccttcttc tctttgtctt tcttacgatt atagcatatt cccaataata
    89161 cctaaaacat atgaaaaagc ggttgctttt gttcaacgat tcacttaaaa taagaatgaa
    89221 aatcattctc aattagaaag gtggattgga tgcgctacgg acgactagta gcattattag
    89281 ttattttaag tagtatttca ttgttcatcg gagtatcgaa tgtacaattg tcagaactgc
    89341 tccagttaga tgggcaaagc ttacaaatct tgctagtgac tcgtttgcca cgaacgattt
    89401 gtttgatcct tgtgggagcg accagtagta tttgtgggtt gatcatgcaa catttgaccc
    89461 aaaataaatt tgtttcacca acgactgcgg gcacgatgga tagtgcgcgt ttaggtattt
    89521 tagtggctat gattttccta cctggtgcat ccttgattgt ccgatccttg acagcatttt
    89581 gctttgcttt tgcaggaaca ttgctttttt tatcccttac gcgattgttc ctccagaaaa
    89641 atcaattgat gcttccgttg attggagtca tgttagggaa tatcattggt tcagttgcta
    89701 ctttttttgc ttatcaattc cagttggtac aaaatatgac ttcatggctt caagggaatt
    89761 ttgcgactgt gatacgagga aattatgagt tattgtatgc tgttgttcca ttgttgctct
    89821 tggtttggct atttgcttac cgatttacga tcattggtct gggcgaatcg tttgcagtaa
    89881 atctgggaat agattaccag aagatgcagt ttttaggcat tggcttagta tcattggcta
    89941 gtgcagtcat gttgatcatg gtaggaagca ttccttttat cggagtgatc attccgaatc
    90001 ttgtctctag aatatatgga gatcaagtgc acaaaacaat tggcgtaact gctttagcag
    90061 gtagtttgtt cttagtattt tgcgatcttg tcgctcgcgt gttgattttt ccttatgaaa
    90121 taccagtcag tgtgatcgtt ggaatcatcg gtggggctat cttcctttat cttctgataa
    90181 ggggacgacg atatgcttaa aaaaaagcca tctattttta ttggtatgct tgttcttgtt
    90241 ttatttgtta caggaatgta cttgacgatc catacatatg gaaactggca ttttgcatgt
    90301 gcattgagga gtaagaaaat ttttgcattt atccttgtct cgctagctgt cagcagtgcc
    90361 acaatcagct ttcagacaat tacgcataac cagtttttga ctccttcagt attgggactt
    90421 gagtcgttat atgtccttgt ccaaacggtc cttttcttct ttatcggagg agtgacgatg
    90481 cttcagcaga caggtacagt ttttttctta ttgaacgttg ggctgatggt cgggctgagt
    90541 ctattgttat ttttctttct tttgaaaaaa atgagagcga atcttttttt attactgatg
    90601 tcagggatga ttttaggtac gctttttggt agtgtcagta cctttttaca agtcatcatg
    90661 gatccgaatg aatatgattt gcttcaaggc agattatttg caagctttgg caatatccag
    90721 agtcagtatc ttttgcctgc ttctttgatc attatcggct gctggtacct gttatttcga
    90781 tatcgacatg tgctagacgt tttccatctc ggaaatgaac aagcagtaaa cctcgggata
    90841 gacacagatc gttttgaatt tttgatttta tgttgtgtca gtcttttgac agggacatca
    90901 acggctcttg tgggtccagt tactttttta gggtttattg tcgcaaatat cagttatcgt
    90961 gtcttttcta cttatcgtca tggtcagcta tttatcggaa gctttctggt agctatgctg
    91021 tttctgacag gaggacagct cgcagtagag cagattttcg gctggaatac aacagtgagt
    91081 gtcgtcgttg aatttgtcgg aggaatttat tttgtcacaa aattgattcg cgaaaggaag
    91141 agtatcctat gattgattta caaaaggtca ataaactgta ccatggaaaa tcagtggtta
    91201 aagatgtaca gttaacgatt cctgaaaaga aattgactgc ttttatcggt ccaaatggtg
    91261 ctggaaagtc aacggtgttg tcgatgatca gtcgtttgat accaaaagat acaggtgaaa
    91321 tctatttaga ccataacgaa gtaaaggctt ggaaatccaa tgaattagcc aaaaaattat
    91381 cgatcctacg acaatcgaat gaaataaatg tccaaatcac cgtgaaagaa ttagtagaat
    91441 ttggacgatt tccttatacg aaaggaaaat tgacgacaaa agacgaggaa atcgttcagg
    91501 aatcactgga acaccttggg ttagaagaaa tagcggatca atcgattgac actctttcag
    91561 gcggtcaact ccaacgtgcg tatattgcaa tggtgctagc gcaagacacg gattatattt
    91621 tactggatga acctttgaac aatctagata tgaattttgc ggttcaaatc atgcagattt
    91681 taaaaaattt agtacaagaa atcgggaaaa caattgttat tgttcttcat gatatcaact
    91741 ttgctgcaag ttatgcagac gaaattgttg caatgaaaga tggacagtta tttacgcaag
    91801 gaacgaccca agaaatgata aaaaaagatg tattggatga attatacgaa atggacatac
    91861 ggatttgtga gattgaaggc cgcagattct gtctttattt ctaaaactaa cattaggaga
    91921 gaagagatga aaaaattagt agtaggtatc ttgattctag catcatttgg tttagcagca
    91981 tgtggaaata gcaacaacac cagtcaggca gacacaaaaa aaagcagcac acaaactgaa
    92041 actacattga cgataacaga tagtaatgga gaccaaatcg aggtgccaaa taatccagaa
    92101 aaagtcgtcg tattcgacaa tggttcttta gatacgatgg atgctcttgg tgttggagat
    92161 aaggtagttg gagcagctac aagcagcctt cctgagtatc tttcatccta taaaaaagta
    92221 gaatcagcag gtggaataaa agaaccagac ttagaaaaaa ttaatcagct tcagccagat
    92281 ctaatcatca tttctggccg acaaagagat tttcaaaaag atctaagtca aattgctccg
    92341 accattttcc tttctttaga tgcgaaaaat ccatgggaat ctttccagca aaatgtcact
    92401 gctttagggg agatctttgg taagcaagaa gaagctaaaa ctcaattaga ggaattatct
    92461 agtgcaatcg atcagacgaa aaagaaagca gaagctactg acaaaaaagc actagtcaca
    92521 cttgtgaatg aagggcaatt atcagcctac ggatctggtt ctagattcgg tttcatccat
    92581 gatttatttg gctttgaaca agcagatgat caaatcgaag catccactca cggtcaaagt
    92641 gtttcttatg aatatgtgct ggaaaaaaat ccggatatct tatttgtagt agacagaaca
    92701 aaagctattg gtggagacga cagcaaagat gatatttctg ccaatgaact ggtagcacaa
    92761 acgaatgctg gtaaaaatca gcagatcatc tcccttgaac cagatgtctg gtatttaagc
    92821 gggggcggtt tagaatcaat gaaactgatg atcgaagatg tgaatcaagc atttaaatag
    92881 cataaataac agaggattaa actctcatac tatgtaacaa taatgtttct tacattctta
    92941 aatagcaaga gcctatcctc aaagaagtta tcaaaaacca tccgtaaaat cgtattgatg
    93001 attttacgga tggtttttat ttatttgaga aatctttttt caacgtgaat tcattatcta
    93061 catcatacgg gttgattcta gaatggggag tggcaaaaaa tagtgtgatc cattttttca
    93121 gcatcaagat cgcctccttt gatttgaatc aaaggtacaa agcgaaactt agataaaact
    93181 taaatttaga tatttttatt ttgtaccgat ctcttcttta aacgatgttc catctgctgc
    93241 ttctcggcaa taagtccact agactagaaa atatgagaag ctattttctg tccagcgttc
    93301 ttattactca aagcaaatcg cagttgtcac aatctcttct aaacgatgtt ccatctgctg
    93361 cttctcggca ataagcccac tagactagaa aatatgagaa gctattttct gtccagcgtt
    93421 cttattactc aaagcaaatc gcagttgtca caatctcttc taaacaatgt tcaatcagct
    93481 gaccttcggt attagctcaa agcactgatt tcacaacctc tatttaggca tgtgtctcag
    93541 caagcggttg atagttgatg atctgcattt ttcgataaaa atcggcggga agcttactcc
    93601 accgttttaa taattgatag aaataactct cttctgcttt gattttatac tcgcttaata
    93661 tcaatccaaa actattcgtt ttttttgaag ctaatttttt ataaatatcc aatgctttgg
    93721 ttttatcttc attgacaaag agtgttttta cttcaatata agaaccctcg atattttgtg
    93781 tttcttcgat aaaatagatg ttttttatat attccttcat agtgattcct ccaaagaagt
    93841 cttttatttg ataagtttac tctaccgaag agttgttaat aatccgtgaa caaaaagtaa
    93901 ttctttttcc aatagttgtt aaaggttttt tattttttat agtatttttt gttatttaag
    93961 aaaggaataa ggttaaacaa ataacaggtt gctttttggg tctttttccg ttcaagaaac
    94021 tgaacgaaga aaagggaaag ttgtctcttt tgtgataaga gaaatcataa taaacgaatg
    94081 acttactttc caataaattg agaacagtca gaaccagaca aattagttgc atctgatgaa
    94141 taaagtgatt attctatgac atgaggtgat acagatgaaa ttagaagtga caaaagaagc
    94201 acaagaagta ttgaagaata aattagaagc agacgatcaa cttttattag atatcgaaaa
    94261 cggtgacggt ccatttgcag aaagccaagt cagttgccaa ttggatacgg catttcgatt
    94321 gatcattgtc aaaaaagata cacaaacaga tctaagtctt tattctgtag tggctgatac
    94381 accagttgga ccaatcaaaa tcaaagacag tgcaatactt tacatggatg atccatccac
    94441 acttgcttta gaaccaactt ataatagttt acaattaaaa ggaccaagcg gcttgagaaa
    94501 aggcaacttg caagttgttc gaaaagacta gagggataaa atgggaaaat tggcgatcat
    94561 cagcgaccta catgtggaca tcaataaatt cggcgaatca gagctgatgc agctttggca
    94621 agttttgcaa aagcacaaga tcactcggct tcatctagcc ggagatacgg ctaataaagt
    94681 agaccgttgc ctagctgttg tcgaattttt cgtcaataaa ggattatcta cgacatttca
    94741 tttcggcaat cacgagttgg cagatgtgcc caatgaacag atgatggacc attttccaga
    94801 cccacatttt ttaaatgaac ggtatattgc tttaaatgag cagaccgttc ttttagggat
    94861 gaatggctgg tacgactatc agttttccga attgaaagat tacgaggcaa tcgtacgaat
    94921 gaagcgtttg tattggtatg atcggttcat ccagcgtggg aaaacagatc tcgaggtcaa
    94981 tgcagaaatg cttgcagctg caaaaaattt gctagaccag ctggaagcaa aaaaacttga
    95041 ggtgatttta tccactcatt tcgttcctaa acgggcattt atcatgtatc aatctgctcc
    95101 ttatgagcgt tggaacaaat taaatgcatt tttaggatca gaatcattcg gagaattact
    95161 tgaccgttat tcaaatatca aacaagtagt ttttggccat acgcatcgtc gctttgaaga
    95221 taaatccatc ggtcacacga tttacagctg cagacctttt ggttattatt atgaatggca
    95281 gctgacccga gattttgtct tgaaagaaca gttgctagac agctatgatc ctatgaaatt
    95341 acgtgtcctc ttgcgtgccc atcacccagc ttttcaggcc tatcaaacac aacatttgca
    95401 agaggaattt gaacaagcga tgacaatcgt tccgtattga gacagcatta gtaatgtgta
    95461 aaagaaaata ccttgtaaaa cataaggata agtaagagca gtagatccat ttatcgtgga
    95521 tatgctgctt tttttgcgga aaataatcag cgaatgttta tttgatcgga tgatttgtaa
    95581 ctcgcagagt actagatgca agcgtgtatt ctttttttga acgataatca caaaaaaaaa
    95641 tata
//
LOCUS       DAZUKZ010000002        76327 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000002 DAZUKZ010000000
VERSION     DAZUKZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.002"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..1092
                     /locus_tag="ACG8EF_000108"
     CDS             91..1092
                     /locus_tag="ACG8EF_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFS4945470.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            1111..2130
                     /locus_tag="ACG8EF_000109"
     CDS             1111..2130
                     /locus_tag="ACG8EF_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFS4945471.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(2232..2948)
                     /locus_tag="ACG8EF_000110"
     CDS             complement(2232..2948)
                     /locus_tag="ACG8EF_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4945472.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(2917..3702)
                     /locus_tag="ACG8EF_000111"
     CDS             complement(2917..3702)
                     /locus_tag="ACG8EF_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4945473.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3693..4442)
                     /locus_tag="ACG8EF_000112"
     CDS             complement(3693..4442)
                     /locus_tag="ACG8EF_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS4945474.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4740..5264
                     /locus_tag="ACG8EF_000113"
     CDS             4740..5264
                     /locus_tag="ACG8EF_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFS4945475.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5280..6779
                     /locus_tag="ACG8EF_000114"
     CDS             5280..6779
                     /locus_tag="ACG8EF_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFS4945476.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6867..7469)
                     /locus_tag="ACG8EF_000115"
     CDS             complement(6867..7469)
                     /locus_tag="ACG8EF_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4945477.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7573..8529
                     /locus_tag="ACG8EF_000116"
     CDS             7573..8529
                     /locus_tag="ACG8EF_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFS4945478.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8834..9790)
                     /locus_tag="ACG8EF_000117"
     CDS             complement(8834..9790)
                     /locus_tag="ACG8EF_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4945479.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            9964..10524
                     /locus_tag="ACG8EF_000118"
     CDS             9964..10524
                     /locus_tag="ACG8EF_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945480.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10517..11341
                     /locus_tag="ACG8EF_000119"
     CDS             10517..11341
                     /locus_tag="ACG8EF_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFS4945481.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11351..11968
                     /locus_tag="ACG8EF_000120"
     CDS             11351..11968
                     /locus_tag="ACG8EF_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS4945482.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            11986..12669
                     /locus_tag="ACG8EF_000121"
     CDS             11986..12669
                     /locus_tag="ACG8EF_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS4945483.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13352..14587
                     /locus_tag="ACG8EF_000122"
                     /pseudo
     CDS             13352..14587
                     /locus_tag="ACG8EF_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            14595..15482
                     /locus_tag="ACG8EF_000123"
     CDS             14595..15482
                     /locus_tag="ACG8EF_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4945484.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15499..16335
                     /locus_tag="ACG8EF_000124"
     CDS             15499..16335
                     /locus_tag="ACG8EF_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4945485.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16338..16961
                     /locus_tag="ACG8EF_000125"
     CDS             16338..16961
                     /locus_tag="ACG8EF_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945486.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            16958..18250
                     /locus_tag="ACG8EF_000126"
     CDS             16958..18250
                     /locus_tag="ACG8EF_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4945487.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18609..18706
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18822..19403
                     /locus_tag="ACG8EF_000127"
     CDS             18822..19403
                     /locus_tag="ACG8EF_000127"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS4945488.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19406..20713
                     /locus_tag="ACG8EF_000128"
     CDS             19406..20713
                     /locus_tag="ACG8EF_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFS4945489.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21006..21494
                     /gene="purE"
                     /locus_tag="ACG8EF_000129"
     CDS             21006..21494
                     /gene="purE"
                     /locus_tag="ACG8EF_000129"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFS4945490.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21487..22611
                     /gene="purK"
                     /locus_tag="ACG8EF_000130"
     CDS             21487..22611
                     /gene="purK"
                     /locus_tag="ACG8EF_000130"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFS4945491.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22624..23919
                     /gene="purB"
                     /locus_tag="ACG8EF_000131"
     CDS             22624..23919
                     /gene="purB"
                     /locus_tag="ACG8EF_000131"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFS4945492.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24099..24476)
                     /locus_tag="ACG8EF_000132"
     CDS             complement(24099..24476)
                     /locus_tag="ACG8EF_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945493.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            24732..25460
                     /gene="purC"
                     /locus_tag="ACG8EF_000133"
     CDS             24732..25460
                     /gene="purC"
                     /locus_tag="ACG8EF_000133"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFS4945494.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            25460..25714
                     /gene="purS"
                     /locus_tag="ACG8EF_000134"
     CDS             25460..25714
                     /gene="purS"
                     /locus_tag="ACG8EF_000134"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFS4945495.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            25716..26387
                     /gene="purQ"
                     /locus_tag="ACG8EF_000135"
     CDS             25716..26387
                     /gene="purQ"
                     /locus_tag="ACG8EF_000135"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFS4945496.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            26388..28610
                     /gene="purL"
                     /locus_tag="ACG8EF_000136"
     CDS             26388..28610
                     /gene="purL"
                     /locus_tag="ACG8EF_000136"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFS4945497.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            28595..30034
                     /gene="purF"
                     /locus_tag="ACG8EF_000137"
     CDS             28595..30034
                     /gene="purF"
                     /locus_tag="ACG8EF_000137"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFS4945498.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            30057..31109
                     /gene="purM"
                     /locus_tag="ACG8EF_000138"
     CDS             30057..31109
                     /gene="purM"
                     /locus_tag="ACG8EF_000138"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFS4945499.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            31106..31684
                     /gene="purN"
                     /locus_tag="ACG8EF_000139"
     CDS             31106..31684
                     /gene="purN"
                     /locus_tag="ACG8EF_000139"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFS4945500.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            31681..33216
                     /gene="purH"
                     /locus_tag="ACG8EF_000140"
     CDS             31681..33216
                     /gene="purH"
                     /locus_tag="ACG8EF_000140"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFS4945501.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            33232..34506
                     /gene="purD"
                     /locus_tag="ACG8EF_000141"
     CDS             33232..34506
                     /gene="purD"
                     /locus_tag="ACG8EF_000141"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFS4945502.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            34628..35542
                     /locus_tag="ACG8EF_000142"
     CDS             34628..35542
                     /locus_tag="ACG8EF_000142"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFS4945503.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(35675..37240)
                     /locus_tag="ACG8EF_000143"
     CDS             complement(35675..37240)
                     /locus_tag="ACG8EF_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4945504.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(37233..38684)
                     /locus_tag="ACG8EF_000144"
     CDS             complement(37233..38684)
                     /locus_tag="ACG8EF_000144"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4945505.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(38698..39714)
                     /locus_tag="ACG8EF_000145"
     CDS             complement(38698..39714)
                     /locus_tag="ACG8EF_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4945506.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            39934..41022
                     /gene="chvE"
                     /locus_tag="ACG8EF_000146"
     CDS             39934..41022
                     /gene="chvE"
                     /locus_tag="ACG8EF_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4945507.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            41076..42602
                     /gene="mmsA"
                     /locus_tag="ACG8EF_000147"
     CDS             41076..42602
                     /gene="mmsA"
                     /locus_tag="ACG8EF_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4945508.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            42604..43809
                     /gene="mmsB"
                     /locus_tag="ACG8EF_000148"
     CDS             42604..43809
                     /gene="mmsB"
                     /locus_tag="ACG8EF_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFS4945509.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            44066..45043
                     /gene="guaC"
                     /locus_tag="ACG8EF_000149"
     CDS             44066..45043
                     /gene="guaC"
                     /locus_tag="ACG8EF_000149"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFS4945510.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            45307..46731
                     /locus_tag="ACG8EF_000150"
     CDS             45307..46731
                     /locus_tag="ACG8EF_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS4945511.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(46773..47030)
                     /locus_tag="ACG8EF_000151"
     CDS             complement(46773..47030)
                     /locus_tag="ACG8EF_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945512.1"
                     /translation="MPIRILTALVNKITIRKAFYFSSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            47192..48301
                     /gene="pdhA"
                     /locus_tag="ACG8EF_000152"
     CDS             47192..48301
                     /gene="pdhA"
                     /locus_tag="ACG8EF_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFS4945513.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            48304..49281
                     /locus_tag="ACG8EF_000153"
     CDS             48304..49281
                     /locus_tag="ACG8EF_000153"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFS4945514.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            49306..50949
                     /locus_tag="ACG8EF_000154"
     CDS             49306..50949
                     /locus_tag="ACG8EF_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFS4945515.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            50957..52363
                     /gene="lpdA"
                     /locus_tag="ACG8EF_000155"
     CDS             50957..52363
                     /gene="lpdA"
                     /locus_tag="ACG8EF_000155"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFS4945516.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            52525..52800
                     /locus_tag="ACG8EF_000156"
     CDS             52525..52800
                     /locus_tag="ACG8EF_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFS4945517.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            52806..53573
                     /locus_tag="ACG8EF_000157"
     CDS             52806..53573
                     /locus_tag="ACG8EF_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFS4945518.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            53741..55570
                     /gene="typA"
                     /locus_tag="ACG8EF_000158"
     CDS             53741..55570
                     /gene="typA"
                     /locus_tag="ACG8EF_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFS4945519.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            55831..57603
                     /gene="recQ"
                     /locus_tag="ACG8EF_000159"
     CDS             55831..57603
                     /gene="recQ"
                     /locus_tag="ACG8EF_000159"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFS4945520.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            57717..58019
                     /locus_tag="ACG8EF_000160"
     CDS             57717..58019
                     /locus_tag="ACG8EF_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFS4945521.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            58022..59185
                     /locus_tag="ACG8EF_000161"
     CDS             58022..59185
                     /locus_tag="ACG8EF_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS4945522.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            59211..62639
                     /locus_tag="ACG8EF_000162"
     CDS             59211..62639
                     /locus_tag="ACG8EF_000162"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFS4945523.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            62660..63784
                     /locus_tag="ACG8EF_000163"
     CDS             62660..63784
                     /locus_tag="ACG8EF_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFS4945524.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            63765..64211
                     /locus_tag="ACG8EF_000164"
     CDS             63765..64211
                     /locus_tag="ACG8EF_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4945525.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            64195..64533
                     /locus_tag="ACG8EF_000165"
     CDS             64195..64533
                     /locus_tag="ACG8EF_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFS4945526.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            64652..65212
                     /gene="rsmD"
                     /locus_tag="ACG8EF_000166"
     CDS             64652..65212
                     /gene="rsmD"
                     /locus_tag="ACG8EF_000166"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFS4945527.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            65209..65700
                     /gene="coaD"
                     /locus_tag="ACG8EF_000167"
     CDS             65209..65700
                     /gene="coaD"
                     /locus_tag="ACG8EF_000167"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFS4945528.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            65690..66733
                     /locus_tag="ACG8EF_000168"
     CDS             65690..66733
                     /locus_tag="ACG8EF_000168"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFS4945529.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            66892..67620
                     /locus_tag="ACG8EF_000169"
     CDS             66892..67620
                     /locus_tag="ACG8EF_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFS4945530.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            67736..67981
                     /locus_tag="ACG8EF_000170"
     CDS             67736..67981
                     /locus_tag="ACG8EF_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4945531.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            67978..68196
                     /locus_tag="ACG8EF_000171"
     CDS             67978..68196
                     /locus_tag="ACG8EF_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS4945532.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            68332..68871
                     /locus_tag="ACG8EF_000172"
     CDS             68332..68871
                     /locus_tag="ACG8EF_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFS4945533.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            68864..71137
                     /locus_tag="ACG8EF_000173"
     CDS             68864..71137
                     /locus_tag="ACG8EF_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFS4945534.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            71175..72221
                     /gene="holA"
                     /locus_tag="ACG8EF_000174"
     CDS             71175..72221
                     /gene="holA"
                     /locus_tag="ACG8EF_000174"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFS4945535.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(72310..72564)
                     /gene="rpsT"
                     /locus_tag="ACG8EF_000175"
     CDS             complement(72310..72564)
                     /gene="rpsT"
                     /locus_tag="ACG8EF_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFS4945536.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(72964..73233)
                     /locus_tag="ACG8EF_000176"
     CDS             complement(72964..73233)
                     /locus_tag="ACG8EF_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945537.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(73495..73623)
                     /locus_tag="ACG8EF_000177"
     CDS             complement(73495..73623)
                     /locus_tag="ACG8EF_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945538.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(73703..74158)
                     /locus_tag="ACG8EF_000178"
     CDS             complement(73703..74158)
                     /locus_tag="ACG8EF_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFS4945539.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(74296..76224)
                     /locus_tag="ACG8EF_000179"
     CDS             complement(74296..76224)
                     /locus_tag="ACG8EF_000179"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4945540.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        24724 a        13190 c        16583 g        21830 t
ORIGIN      
        1 tgtgaataaa tgaacataat taggaggaga aagatgaact tatttgacat ttttcgatcg
       61 actttgacag atatgtcgat tgtcagtgca atcacttcaa cagtttttat tatattatta
      121 gggtttgttt gtagaaaaaa ggggattttt tctgaatctg ttggaaaaat tctatcgaaa
      181 gtcgtcttaa cagtggcttt gccagcactg gcatttcgag ctttcatgca agatatcaat
      241 acagaattac tgatgcaagg aatcaatgtc ttgatttggg gattagttat ttatattatt
      301 cttattctag tttcaaaacc ttttttctta agctataaag gggataagca aacaacgttg
      361 cgtgtcctga cgatctttgg ttctacgacc tttttcggaa cgcctatcgt cagtgcgatt
      421 tatggtcctg tcggcgtgat gtattcctca attttcaata ttggctaccg aatctttttg
      481 tattcttatg gatacataaa aatgagtggc ttgaagatgg aagctaaaaa tatcaaagcg
      541 atgttcgtca atccaatcat tatcgctact tttgccggcc ttttcatttg gctgttccaa
      601 gggtatctgc cacaagtgtc agttacaggt gcagaaggag agataagcca agtagcgttt
      661 ttgcgaattg accagactgc accgtggcta tttcagccga tggattattt agctggttta
      721 agttcaccat tggcgtggct agcaatcggc gcaacattag gtgaagtaag cttcaaaaaa
      781 gctgcatctg aaaaaacttc atggtattac agtctagtca aagtggtatt tgtacctgca
      841 ttgaatatcg tgattctagc agttttgacg gcaacgaata ttctaccagt caattttgaa
      901 gcactggcaa caatcgttat catgatggct acaccagctg caacagtggc agcagcctat
      961 gctatcagtt ttgataggga ggcactttta gcctccaatg catccttact gtcaactatt
     1021 tttgctgtgg tactgacacc tgtatggatc gttgtattac aaatgattca tggcataggt
     1081 ttattttcat aagaaatgga ggaagaaagt atgagaatcg catgttacgg tgttcgtccg
     1141 aacgagatag atttttttga aaaattgaat atttaccatt atgatctcag tctttatgaa
     1201 gaactgttga cacatgacaa tatcgtgtca gcaaaggacc atgatgctgt attgttgagg
     1261 ggaaattgta tagcagatga gaccaatctt gcaaaaatgc aggaatatgg gattcgttat
     1321 gtttttacac ggactgtggg agtcaatcat atcgatctac aagcggcagc tgattttggt
     1381 atgacagtag cccgtgtacc atcgtattcg ccaaatgcga tagcagagtt atctttgact
     1441 tttgccatga tgcttttgag aaatactgcg tatacgacga tccgaacatc tttcaaagat
     1501 tttcgagtgg atgagcaaat gtttagtcgt gaaatcagaa attgcacagt agggatcata
     1561 ggaacaggca ggatagggtt gacggaagct gttttgttta agggactagg agccaaagtg
     1621 atcggctacg atattcatca gacagaagct gctaaaaaag tattgacgtt ccactcgctt
     1681 gaagagttat tagccaaaag cgatattgtc agtattcatg tacctcatat cccaggtgaa
     1741 aacgatcaaa tgatcaatga aggattttta gctcaaatga aaaaaggtag catcttgatt
     1801 aatacagccc gtggagagct tcaagataat gaagctattt taagagcttt gaaaagcaat
     1861 catttagctg gttttgcaac cgatgttttt gcaaatgaaa cagaggtttt tttccgatcc
     1921 ttcaagccat gggagacgat tccagaccct gctatccagc aattagtaga aatgtaccct
     1981 agagttcttg taaccccgca tgtaggttca aatacagatg aggcattatc aaatatgatt
     2041 tcgacaagtt tcgagaactt tcgtgaaata atagaaactg gaaaaacgaa aaatgaggta
     2101 tctcttccta aagcccgtca attaagataa gaaaacatgg atattcgtca ataaaatgtt
     2161 taaaaatagt ccaagtaaac agttgttact tctgtatact tggactattt tgtatgggat
     2221 aattcgatga ctcaacagta atctgtgagc tttttccgcc cattctttgt caattcataa
     2281 caggttagcg gctggccgat ttccaaatat ttgattcttt cgttcaatat atcttgttct
     2341 actaaaaaat tcaaatattt cttgacggat atacgtgaca gtgagctttt ttgagccagt
     2401 tcttttgttg aaaaaatagt atcttctttt agaataaatg cacttatttt ttttaaagtc
     2461 agttttgaca gacctttagg caaatcgatg aatgatggta tttcgtgaat gggagtggtg
     2521 tggttgaata cttgatcgat caatttctga tcggcttttt cagtcgtttg gattgtctga
     2581 gagaaagcaa gatacttttc gatcgccagc tgaaaacgtt gcaaagtgaa cggtttgatc
     2641 aaataatcta aggcaccgta gcggatcaca ttctttatgg tttcgatatc atctgcagca
     2701 gtgatcaaaa tcactgggat atcatttcct tgctgtctaa gccaagataa aaaatggata
     2761 ccactttcgc caggcatgta tacatcaagc agcagcagat ctgcgttttt ttgtctgaaa
     2821 tatttttttc cttcttctac atttctgaaa ctttcgatta cgttcatatt agggatgctt
     2881 tccacaaaag tacgattcaa ttctgcaacc attggatcat cttcgattat caggacattt
     2941 gtcatcattt tgatcttcct tttttggtaa ttctagatag acgattgtac cgccatttgt
     3001 ttgtttggaa aattctaaaa atccaccgtg gcgtttgacg attgaatgga tgagattcaa
     3061 tccaaaccca cgattgattc ccttcgtcga aaaacctagc gtaaatattt tttctgagat
     3121 ttcttctgat atcccgacac cgccatcgca gacggtcacg atgaatactt cagcttctgt
     3181 gtcatagttt aaatgaatcc ggacctgtcc gtttctttca tgtttttgta gcacggcttc
     3241 ttttgcatta tcaagtaagt tccctaacac ttgcaaaagt gaatggatgg agtcatccat
     3301 ctccatttta gggataaaag aatcgctttc taacagcatt tcaacagatt gttctttggc
     3361 ttcgtttatt ttgcctaata aaaaaccggc aattgcaggt actttgatca gacttgagac
     3421 ttggccaatc tcttttttat aattttgatt tagctgctgg ataaaatggg tgacttcttc
     3481 atacttcttc aaatcaatca aaccagaaat gacatgaagt ttattcatga attcatgggt
     3541 ttgagctctc atggagttta tatattgttt cgtcccccca agctcctcca ttaatttcat
     3601 tacttctgat cgatttcgta aagttgcgac tgctcctaaa aaacggtcat tgctaaaaag
     3661 aggtgtaacg ctagaaatag tttctatccc atttaaatac atggattgag tccgaacggt
     3721 cgtttgatgc tcaatcgtct ctttaaacaa aacgtcatat agattatcac tgattttttt
     3781 cctacttcga aatccaatag ctgctctgtt tggtagaaaa tgttttttgc aggtgtattg
     3841 atcaagagga tagttttatc agaagagatg gctaagattc cttcatctac ttcattttcg
     3901 ataatcgttt tttcacttaa ctgttctgct atttcttgtg gttcaagtcc aagcagtact
     3961 tttttcattt tttgggcaag aaaaacggcg cctatgatgc ctacgctaag tccaataatc
     4021 aatgaaaaaa caatcatttt ttgtccatct ttgacttgat tgtcaatcgt gttgtttgtt
     4081 aagccaacac aaactgcacc aataatctct tgatcataat aaatagggac gaaatagcgg
     4141 aagccgaacc ctaacattcc ttctttttca gagaaaaatt cttgtcctcc aatcactttt
     4201 ttcacatctt catgacttga gaaaggaaga ccgatgtaac ttgggtctgc gtgagaatat
     4261 cgaatgcctt cagggtccat gacaacgata aaatctaaat tcgatgcaat catgatttca
     4321 tttgttattc cttgaatcgc atgtgtcggg tcaccagtct gcagcttttt ttggatgagt
     4381 ttatcattgg caacgatttt agcaatgcta gagattttat ctgacacact agatcggttc
     4441 atcgaaagaa taaaattcct gatcaatatg gcagaaacaa tcaaagaaac gaatgtggag
     4501 aaacaaacga caaggatgat cgtgttctgg agctttaatg gttttttctt aaaaaaagga
     4561 aacataggct acctcgaaag aatttattgt gaaaatttta acatgaaaac aaaaatttaa
     4621 caaattgtca acgataaaca caaccttcct ataaaaaaca ccactgagtc ggtaaaagaa
     4681 gctatacaga attttcaaac attggtatga tagacgtaga aatggaagga ggcgtgtata
     4741 tgataggggg aatcgcactt gagacgattt attggtatac attgctgatt tgtgcaggat
     4801 tggcagtcct attgattatt tttggagatg tattcgattt tgatggcccg cttgatccga
     4861 tgcttattat cccttggtta actttcacaa gcttatttgg ttttctaggt gagcgtttaa
     4921 cagatcaatc atctttattt ttatttattg ctagtggagg catatcgata gtgttggtat
     4981 ttttgcttaa tttctatata ttgatgccga tgaaacatgc cgaatcaacg atgtcgtctt
     5041 ctgaaaaaac tctggaagga caagtagctg ttgtgatcac accgatccct gtacgaggta
     5101 tgggagaaat tcagttgaaa agtgttaccg gttccattag tcgtccagct tgtttttaca
     5161 gcccccaaga acagacagct gagcggggaa cgaaagtatt gatcattgag atcaaggaaa
     5221 gaatctgtta tgtcatgcct tatgaaagca tgataaaagc ttaaaggagc gtgtgttaaa
     5281 tggaaatacc agttgttttt atcgcgatcg caatcgcagc tttgatttta ctgatgttgc
     5341 tgatcgtctt tgtagcgaag taccagacag caaaaccgga tgaagcgttg atcatcagtg
     5401 gaagttattt aggaagcaaa aatgtgcaca ttgatgaaag caataacaaa atcaaaattg
     5461 tgcgtggagg tggcgcattc gtcttgccag tttttcaacg ttcgaatcgg atcagtctat
     5521 tatcgagtaa attggacgtc tccacccctg aagtgtatac cgaacaaggc gtaccggtga
     5581 tgtgtgatgg tacttcgatc atcaaaatcg gttcatctgt agaagagatt gctacagcag
     5641 cagaacagtt tttaggcaag acaagagaag aactggaaaa tgaagcaaga gaagtattag
     5701 aaggacactt gcgctcgatt cttggttcta tgactgtaga agaaatctat caaaatcgcg
     5761 ataagttcag tcaaagcgta caagaagtcg cttctgtaga ccttgcaaaa atggggttga
     5821 tcatcgtttc tttcactatc aaagaggtca gagacaaaaa cggttatctt gactctttag
     5881 ggaaaccaag aatcgcccaa gtcaaaagag atgcagatat agcagaagcg gaagctctaa
     5941 aagaaacgcg tatcaaaaaa gctcaagcag aaaaagaatc acaagcagct gaattacagc
     6001 gacaaacaga gatcgcagaa tcattaaaag aaaaagaact gaaattagca acctacaaac
     6061 aagaacaaga tgttgctaaa gcaaaagcag accaagccta caatttagaa agtgcccgag
     6121 cacaacaaca agtaatcgaa caggaaatgc agattaaaat cgtagaacgc caaaaacaaa
     6181 tcgaactgga agaaaaagaa atcacacgtc gtgaaaagca atatgattcg gaagtgaaga
     6241 aaaaagcaga tgccgatcgg tacgctaaag aacaagaagc acaagctcaa aaagtcaaag
     6301 aagtgactga agccgaagca gaacgattcc gtgtggaagc actggcagaa gcggaagcca
     6361 ataagacacg tttagctgga caagcagaag ccgaagcagc actagcaaaa ggaaaagcag
     6421 aagccgaagc aaaacaaaag atcgccaatg ccttcaagga atatggcgaa gcagctgtct
     6481 tgagtatggt tatcgatatg ctaccgcaat tgatgcgcga agcagcacag ccgctaggaa
     6541 atatcgaaaa aatctctgtt gtcgatacag gaagcagttc aggagaaacc agtggagcga
     6601 accgagtgac aaattatgcc acaaatttgc tttcttctac tcaagaaaca ctaaaagcga
     6661 caacaggact tgacttgaag gaaatcgtag agaatttgtc gacacgtggg actactagcc
     6721 aagtaaatat gtatgaagcg ccaaaagagg cagaacaaaa ctcgatgaag gaagaataaa
     6781 gtagtacact tcaccgttca atcaaaaatg gaggaagact ttttaaagtt cttctctcca
     6841 tttttttatt ttattgcaaa agaaaattag tcctcgctat ttttcttttg tttttggatc
     6901 ttagcttcta tctcttgtaa atttgttctt agcaatcgtt ctttcttttg gagttcgacc
     6961 aaatcttctg atagttcttc cttcttttct gtttcaggag gacgaagaaa gaaattgata
     7021 ccgattgcaa tgaatgtccc tataaaagta tctagtacac gttggatagc atacaaagcg
     7081 gattcgcctt gagggatgct caacgcaatc agcaacaacg tagctattgc ggaaatgata
     7141 ccggaattat tgttaatacc atctgacacg acgatcacaa taataactag aataggaagc
     7201 aagatcaatt ctaccaaaaa atcattgtga aaatagcttt tgacaaaaaa ataaatgata
     7261 gctaatgtac cacctaatgt attgccgatg atcctagact ttccaaaaga aacggttgta
     7321 gtcaagtcct gacgcaaaga aaaaacagca gataatgcag caatcatcgg gattcctcgt
     7381 tgagtcaaat gaaaaaacaa gatacataaa aaaacagcta aggcactttt gatcgtacgc
     7441 atacctagtc gaaaacggcc aaattgcata gagattctcc tttttggtct ttttactatc
     7501 atacctaaac ttttgtctga tagctagttt ttcattattc aagtataatt aaaataaaaa
     7561 agggggaagt aaatgcgtat aggaatgcga acagtcaaaa cagtgatcag tgtcttcttt
     7621 tcgatggtca cagcgggtag cttatcgctg ttgtattggc cagctgcagg gatcattgca
     7681 ttattaagtg taggaaatac caagcgctct acattgcgta cgggcatgaa tcgaatcgca
     7741 gcctttatat tagctacggc aacagcactg ttcagttttt ttgctgttgg gtatactatt
     7801 tttggcttca gtcttttttt attcctgttc atcccgcttg cctctcgctt caaattaacc
     7861 gaagggatag tagtaaattc ggttttggtc actcattatt tagtggagga aaacatgtct
     7921 tggcagctga tcggcaatga agcgggatta atggcgattg gattagtatt tgctttgctt
     7981 gcaaatgttt acatgccaga tcgtacgaag cagctgaagg aaaaccaaat acagatcgaa
     8041 aaagaatttc gaaatatttt gagacaaatg gcagaatttc ttttatcaga aaataaagaa
     8101 gatgtgcaga tcaaatgcga acatttactg acattcattc gcgaaagcca agaagatgcc
     8161 agagaacatc aagaaaacta ctggctcaga cagccgctat actacgaaac ttacttctct
     8221 atgagaagag cacaggccaa tgtgattaaa gacatgctgg aaaatctaga gagaatccag
     8281 caaccagctt attatggaaa acatatctat ggtttattga tttatactgc agaaacattt
     8341 tcagagtcaa atgatggcag acaaatttta acacgtattg aagaagttta tgtattgtat
     8401 cggcaaatgc ctctgccgac tagtcgccct gaattcgagg atcgagcaga attgttccag
     8461 tttttgcagt cttttaaaag ttttattgaa atcaaggcag aattctccca acaaaaaatc
     8521 caaaaataga ggttgtgaat tcagctaata ttcaggtcaa aaagctgatt tcacaacctc
     8581 ttctttaaac ggtgttctcc tgtcaactcc tcggtcacaa gtcacaatat tcaacaaaca
     8641 tgagaagctg ttttttgaaa ttgctccttg tgactcggag ctgaacggta ggctcacaac
     8701 ctctttctta aacggtgttc aaaagctttc ctctgcggta ataagtccag ccaaacgaaa
     8761 aataggagga gctatttttg tttggctgtt cttattactt gatgcagaac agctttttca
     8821 caacctttta aaattacata tttttccata gaacttgcat ttgatccaag atttcgatga
     8881 catcttttgt caagaagaga gagcgattgg gcaagtcacc ttcaatcatg tgtaccatat
     8941 tctttatttc gtaattcatt gcttcagatg tattcccgct ttcgatgaat tcctttgtac
     9001 catctgtaaa taagatctcc gcacgatcag cacgcgggta atcagcaatc gtcacataac
     9061 cgttctcaaa ggcaacgata ccgacttttg gcatttttgc ctgaaaagtc aagctgacag
     9121 cagcaagttc attttctttg ttacgcaaga tagtgacgga ttgttcgtcc acgcctgttt
     9181 caaaaggaac cattgtagag gcaactactt caggttgaga gctcaaaaag aaacgagcga
     9241 aagatacagc atacgtaccg atatccaaca gagctcctcc agctaattct ggattgaaga
     9301 accggttttt agggtctggt tctttgtaac tgccgaaagg tgcttggatc atttttaaag
     9361 cccccagttt tcctgtatcc attattgagc gtaactgctg ataaagtggc atgttgaaga
     9421 ttgtcatcgc ttctgctaag atcaaattct tttcttctgc taagcgcata gcgtcagcca
     9481 gttctttttt attcatcgtg atggcttttt cacatagaac gtgtttgcct gcttccaatg
     9541 ctgccaagat atggtcgata tgctgtctat ttggtacagc aatgtacacg atgtcgattt
     9601 cagcatcgtc taacatttct tgatagcttc cgtaagcttt tggaatattg taacggtgag
     9661 cgaagttttc cgctttttct gaagttctgg aagcaactgc tgttagttcg ctcgtctctt
     9721 gattgaagct ttcggcaaac tcatgtgcga tggtaccaag accaacgatt ccccattgat
     9781 attttttcat ctgaaaacgc tccttcatag ttggattgag tttcttgtct gtctattatt
     9841 ataacaaaaa tcacgaagaa taaagaggag aggacttgaa acattaaaaa aaatgaaagc
     9901 agcgatttaa gaggtgcttt ttgatcaaaa cttaagtaga atagttaaaa ggaggggata
     9961 aacgtgcatc aagtatatat tgatcttatt atgagtgtat tattagaaca gtttgaaact
    10021 gaaaaaaatt ttttagatga atacttgctg atcagtccag aacaatggca aaaatggaaa
    10081 gatggcgaga aaaatctgcc atctgaagcg atgcagaaag taaaaaatct tttcactgat
    10141 tatgaatgga tgctaacgca aaagattttg cgtcaaacga ttatttttcc cgaaaaacga
    10201 aacgttgccg ttgcagagta caagcaaatc aaaacacgca tcgcgcaaaa atggcttagc
    10261 agcaaagcag caaaagtcga gctgatccct ttgaaaaata atcagcaggc tggtagatac
    10321 cttgacttga aagtaagtat tcaatatgat gaatggggct atgatgatat tttgaatttt
    10381 cgtctgccag cagatgttca aaaacagatc gaaggagaaa aaatcgaact tctcgattgg
    10441 gtgaatgaaa atctggaaga aacttacgtt catgaggcga atggggaacg tgaagaacaa
    10501 cgaaaggatt tttcaagtga ataaattttt aggatttgga gcagttgtga ttatgatcgc
    10561 agcaatgggc tatgttattt ttagcgatga tgattcgcat gttcaagccg gcaatcaagc
    10621 agtcgaacga acaagcacca aaacaactgc tagtaaagaa aagaacagtc aaacaaccag
    10681 cagcaaggta aaggacaaag aaacaaaaga tcgtacgtcc aaacaattgc cagatgttaa
    10741 aacagacgat tggaagctag ttttggttgg tccagatcac aaattagaaa aagaaattga
    10801 tgagtctact caattggcat cattaagtaa cggatacttg atcgacaaac gcattgctcc
    10861 agaatatgaa gcatttgcca aggctgctga agcagctgga tttccattag tgatggtctc
    10921 agcttaccgg tctgtatcat cccaacaaca agttttcgaa caaaatgtcc aagatgtgat
    10981 gagtcgtcaa ggtgtatcag aagaggaggc gacaaagatt actaaagaaa cgattacgga
    11041 gccaggatac agcgaacacc acacaggtct ggcagtcgat gtcgtcgatc aaaactggta
    11101 caacagttat ccttctactg tattggatgc aagttacgga gatcagcctg gagctaaatg
    11161 gatcgcagat aatgctgcgc agtatggatt tatcgttcgt tatccaaaag gacacgaaga
    11221 tataactaag atcacatatg aaccttggca cttacgttat gtcggtaaag aaaacgctga
    11281 gtatatcacc aaacaccaat tgacgctaga agaatatctg aatgaattaa atgaaaagta
    11341 ggaattgatc atggcgaaaa aaaagtggaa aaagcaaaag cgcaagaata tccgttggcc
    11401 agcagtggtt gccggtgtag cagtgatact cttagccttt gttttttcct tgaagaatct
    11461 gtcttctcct tttactgata atcagcaaga aaatgaagcg caatcccatc agcaatttat
    11521 tgaacggctt gttccgcatg cccaagaact acaagatggt tatggagtgc ttcctagtat
    11581 cattttagga caagctatat tagaatcaaa ttggggaaaa agtaccttgg ctagcaaata
    11641 caataatctt ttcgggatca aggcttatgg ggatcagaag aaagtaagcc tggaaacaaa
    11701 ggaatttgtt aacgaagaat ggataactat ccaaggagac ttcaaagtgt atgattcatg
    11761 ggaacagtcc atggatgacc atacacaact gttcgtccaa ggagtagatt ggaatcctgc
    11821 gctgtatgaa aaagtgatta cggccaccaa ctatcaggaa gccgcacaag cattgcaaga
    11881 tgcaggttat gcgacagacc ccggttatgc gcaaaaaatc atccaggtca tcgagacgta
    11941 tcaattgaat cagtatgacc ggagatagca aaggagaagt ggctgatggc agaacaattt
    12001 acgccagaaa tcatgacgga attgagaaaa gatatcgtaa gagtaccgga aatcatttta
    12061 gaagcaagcg ggatcaagat atttggaaaa ttgatccgtt cgatcatctt tactacggat
    12121 attgcgatca tacgtaatac aaatgcgaat gcagtaattg ctgtgtatcc ttttacacct
    12181 catccagcga ttactaaaag cattattgag gcagctgaca ttccagtttt ctccggtgta
    12241 ggtggtggtt tgacacaagg tgctcgttct tcctacatga gcatgttcgc agaagcgcaa
    12301 ggttcgttag gggtcgtttt gaacggtcct acgcctttag agacagtaaa agaagtttgt
    12361 cgagtagttg atattccagt tatcagtacg gtaacgtcga aatatacaga aatagatgaa
    12421 aagttagcag ctggtatttc gattatcaat atcagtgcag gtaaagaaac tgcagaagca
    12481 gtacgccatt ttcgtaaaaa atatcctgaa cttcctatca ttgcgacagg tggtccaacg
    12541 gaagaaagta ttgctgagac cattcaagca ggagcaaatg ccatcacata tacgccacca
    12601 agtagcggag agctttttag tcgtaaaatg gaaaaatatc gggaaactga gaaaaagaat
    12661 catgaataac aggtgagaat caaaagacga ggaagagcag atagtggctc ttcctcgtct
    12721 tttgtactcc agtactattt gttcagaatt caaaaaaaag agaaattttt ttgttttttt
    12781 acctgtaatg tacataagaa gagttaaaac cttttgttat tattagcatt ctaacaaaaa
    12841 aatgtcttga tttattgaag cgctttctat agaatgaaaa acgaataaaa actaaaaaag
    12901 gcacccgaca atttagtagc agctaaattg tcggggcttg aaaagtcagc gggaactgac
    12961 caatcaagtt gccatagtca atgcttgtgc atcgacgtgt atattgtacc taatttcata
    13021 cagaattact agttattttt ctgtttttta gggaagagcg cacacgaaga agcatgctta
    13081 tttagctatg tcaaggatat tgggcagttg tctgatatcc ttttttcttt gcgtaattat
    13141 cctcccatca ttgaagcaag aagtgttttt agcgatgaaa gaagaatcag cactatctaa
    13201 tttttcttga aagtattgag ttgttcatcc taggcagaac gataaggcaa gtcttggttt
    13261 ttattcccct tgaaaaagat cgtttaataa atatgttctg tataaaaata cagtatattg
    13321 aaaaataaca atggaagtag aggtgcagag aatgaagaaa agaatgagaa tcatcttagc
    13381 tttaagtgtt atgttattct tcttgacggc ttgtgggcaa gaagatgaca aaaaaatcac
    13441 gctgaacatg gcttggtggg gttcgcaagt ccgacacgat gcaacagtaa aagtaatcga
    13501 gctttatgaa gaaaaaaatc cccatgtgac gatcgattat gaattttatg attatgaagg
    13561 atattacaca aaattaggta cactagcagc atcagatgat atctgggaca ttttccaact
    13621 gaacaatcaa tttcctcaat acatcaatca gattgaacca ttagatgaat atgtggagca
    13681 agggataatt gatatttcta atgcagatga atcctattta gcaacgacga cttacgacgg
    13741 gcaattagtt ggactgtcta acggggtgaa ttcgtttgcg attgcttaca ataaaaatat
    13801 atttgaacaa ttaaatatcc ctgaaccagc ttcaaattgg acatgggatg aatttgaaca
    13861 aatcagctat caaatcactg aagaattagg aatctatgct atttcaagat tcgaagattt
    13921 tatagctggc tgtattattc aaataccaca agttgagaaa ggattgaatt tttataatcg
    13981 ggaagatcta tctaacagct tgggatttga aaatccagac tatctaacag attttttcca
    14041 gctgcgtaaa aatctggtag acaaaggggc ccatccagaa ccaggtggtg gagctgcaag
    14101 tacgacggat ctgtcttttc aggcggttag ggatagagaa gcggcaatgg tgtttctatc
    14161 aagtaaccag ttgacagaaa tattgtcagg ggctcctgaa gatatggaga ttcagttgat
    14221 taatccacct cgcagaaaag cagatggaga atctggtatc cctttacgtt cttcgcaaat
    14281 gctgagtatg gcaaaaagct cagcccataa agaagaagct gctaagttta ttgatttctt
    14341 tcaaaacagt gaagaagcca atgaaatttt aaaaggagaa cgtggtgtac caatcatgtc
    14401 gaatattcga gaacagctcg ctcgtgcaga tgatgcaact ttgcaagcga catttgatta
    14461 tttagaaatg atttccgata tggacaacgg cgaagtgaat gtctttgata gtgctttgaa
    14521 tccggaaatt caagaccaat acaatctttt agttgaaaaa gtcatttatg aagaagtaac
    14581 agcagaagga aggcatgaaa aaagaaaaac taaaacggtt cttggatcaa gacaatgtgg
    14641 tcggttacgt atttgcttct ccattcatcc taggatttct attgataaca gtgataccga
    14701 tgatcttgtc actttattat tcgttcaccg attatcgttt gggaaatcca gtctcatgga
    14761 tcggcttgga taattatata aagatgctga gtgatgcccg ttttttgaac tccatccgag
    14821 tcacttttct ctatgtattt gcatctgtac ctgtgaaact gatctttggt ctattgattg
    14881 cttatctttt aacgaggaat atcaaaggaa cgactttctt tcgttctttg tattatgtgc
    14941 ctagtttgat cggtggcagt atttctatcg ctctagtttg gcgggagttg ttttctaaaa
    15001 acgggttagt gaatattttc ctggaacgat taggcgcacc tgcgctttcc tggtttagcg
    15061 atcaaaaact ggtcatgatc cctcttgttt tgatgtctgc ttggcaattc ggttcggcaa
    15121 tgatcatctt tgctgcaggt atcaaagaga tcccaggctc gtattatgaa gcggcagaga
    15181 tcgatggagc gaacaaatgg cagacgtttt acaagatcac gttacctctt ctttcgccga
    15241 tcattttgta taacttggtt atgcagacaa tcgcggcgtt tatgactttt acgcaggcat
    15301 ttatcattac ccaaggtggt ccgaatgatg ccacgaactt ttatgctctt tatgtgtata
    15361 accaagcttt ttcttttaat tctatggggt atgctagtgc tatttcgtgg atcatgctgt
    15421 tgattatggg agctgtcacc tttctgattt tcaaatcatc aaaaaaatgg gtcgttgtgt
    15481 gaatgagagg aggaagaaat gaataataca gtaacgaaat ggtttcaata cggttttatt
    15541 ttgctattag gtctagtcat gatttatccg gtcttatgga tgatcgcagg ttcttttaaa
    15601 acggatcaag agattatgag tggttcgctg aatttgattc cagagacttt tcaatgggaa
    15661 aattacgcaa aaggatgggc ggggtttgct ggaatttcat tttttacttt ctttaggaat
    15721 tcgtttgtca tctctgcagt ttcgacgata gggacagttc tgagctcgac ttgtattgct
    15781 tacgcattat cgagaatcga tttcaaagga aaaagattct ggttcgtcgt catgttagta
    15841 acgatgatga ttcctgcaca agtgattttg attccgcagt ttattattta caatcggctg
    15901 aatttggttg ggacgtatgt gccgctgatt ttaccacatt tttttggaca agcatttttc
    15961 atttaccaga tcatgcaatt catggtcagc atccccaaag agttagatga atcagcgatt
    16021 attgacggat gcagcaagta tagtgtcttt accaaaatta tttttccttt gctgaaacct
    16081 tcgatcatca cgacgatcat catccagttt tattggaaat gggacgactt catgggacca
    16141 ttgatttatt taaacaagcc gagatcttac acggtatcga ttgccatcaa attatttgca
    16201 gacgctggat ctagtacgac ttacgcgagt atgtttgcga tgtctacgtt gtcgctgcta
    16261 ccggtttttc tgattttctt attcttcaat aaatatcttg tccaggggat cagtacaagc
    16321 ggtctgaagg gctgaatatg aaaaagccaa tagaagagat gtttctttac aagctcaact
    16381 actatgtctg tcggattttt ttaaccagtc ttatcagtaa tgctcttttc gctttagttc
    16441 taggatgttt gttcacttta ggattagtgc tgtctgctcg tgttgtctat ctgatttatt
    16501 ttttgatgat gtatccttct tattatctat ctgtccatta cttggcagaa aaaaaggtat
    16561 gttggaggaa agggaaaatc aaacggatgt gtttcatcat tacgttgttt cagctgtttg
    16621 tcattatttt tgcagttgat tggtatttat tagtgggaaa gattggagct tggtatctga
    16681 ttccttttct attgtgtctg ttgctttttg tatcccatag tttttttagt acgctaattg
    16741 atatctgcaa agaagataaa gcagatagtc ttgtttattt gatcaaaagg aatatctggc
    16801 tgttgtcaaa aaacgtcaag gggtttctgt ggtatttatg ccttttgtat ggactgttct
    16861 ggtgtataca gcctgatttt ttactcactg cattactgta tccagggctg ccatttgtca
    16921 cagtattttg cttaaaagaa aaggagaatg actttaaatg aagaaaaaat atgtacaagt
    16981 cggacttggt gggagagcgc gtttttttta tgaggcaatt gcctctacgt atcatcaaac
    17041 ttgtgaacta gtcggttttt gtgatgtatc tcaaacaagg atggattatg caaatcggat
    17101 acttgtgaac aagtatggac agaaaaaagt aaagacatgt atgccatcac aatttgagca
    17161 actactggat gacgtacggc cggctgaagt cattattacg tcagtagatc gaacgcatca
    17221 tgattatatc attcgtgcaa tgaaaaaagg atatgatgtt atttgcgaaa aacctctgac
    17281 tatcgatgag gaaaaagctc aagaaataat aacggtccaa aaacagacgg gaagaagcct
    17341 gcgagtcact tttaattatc gttatgcacc gtatcacacg aagataaagg aactgataga
    17401 aagtgatgtg attggtgatg tgttttctat ccacttcgaa tggctgttaa atacgcaaca
    17461 cggggcagat tatttcagaa gatggcatcg aaataagcac aatagcggcg ggcttcttgt
    17521 tcataaatct acccatcatt ttgatctagt gaatttttgg ttgaacagcc gtccccaaaa
    17581 ggtatttgca ttcggtggat taaactttta tgggatgcat aatgcggaga aacgcggagt
    17641 agaaatattt tacagtcgat gctatggaag cgaactagct aaagctgatc catttgctat
    17701 agacctgtca gccaatgagg aattgcgaga aatgtattta gaagcagaaa aagacagtgg
    17761 atatattcgt gaccgcagtg tattttccga tgatatctct attgaagata cactaggcgt
    17821 aacggtcaaa tatgaaactg gtgcgatttt gacgtattcg ctaaacgcgt atatgccgtg
    17881 ggaaggattc aatgtttctt tcaatggcag caaaggacgg atcgaaatga aagtgatcga
    17941 aaagtcttac atcaatgcag gtggagataa aaaggatgaa ggagcagccg ttcttcagtc
    18001 catccatgtc tatccgatgt tcggtgagcc atatgaggta atcgtcgaac aagaagcggg
    18061 cggacatggc ggaggagatc cgcgtttatt agatgatcta tttggtgaag aaaaagaaga
    18121 agatccgtgg aatcgggcag caactcacgt agatggtatt ttgtcgattt tgacaggaat
    18181 cgcagcaaat cattctattg cttcaggaaa agcagtagtg attgacgagc ttgtcagctt
    18241 aaaagaataa gaatagattt acggaaatta cttcccgtag tttataaaat cttgccttat
    18301 tgccgatagg ctgtcttttg aacactattt attcggaaaa aggggctatg acatgctttt
    18361 tgtcatgcct tctttttatg tgggctgctt ttttagatag gagaaaataa aaataactat
    18421 ttaaacccga acaaaaagga ataatgcatt aaaaatcatt agattttaca aaaaaataag
    18481 taaaccacgt tgtcagtttc agaaagtagt gttagactgt tttcaatatt tgaaacaaca
    18541 ttttttattc aaatatggca aactgccttc acgtgcaaaa cagttgcaag aaaatgaatt
    18601 tacactatta ttagttttac atacctactt atatatcgtc ataatatgga tgacagtttc
    18661 tagccagtac cgtaaatgct ggactataag taaaagattg gctatttcac ccttcttttc
    18721 ttatgataaa gtggagggtg tttttcttta acagaaacat agtaggtttt tgcacaaaag
    18781 cggttatttc ggtattaata aatgataaag gagcaaataa agtgaaagaa ttagttgaac
    18841 gtattcaaaa agacggacgt atattaggcg agggcgtatt aaaagtcgac agcttcgtca
    18901 ctcaccaagt agaccctgta ttgatggaac aaatgggaaa acggtttgca gaagtattta
    18961 aagaacaagg aatcacgaaa gttgtgacca ttgaagcttc tgggatcgca ccagctttat
    19021 ttgctgcaag agaattagat gttccaatga tcttcgctag aaaagcaaaa agtttgacga
    19081 tggatgaaga attattgaca gcttctgttt attcttttac gaaacaagtg acaagtacga
    19141 tttctatttc tcgtaaattt ttatcttcag aagacaaagt attgattatc gacgatttct
    19201 tggcaaacgg acaagcggca aaaggattga tcgaattatg ccagcaagct ggagcgcaag
    19261 tagaaggaat cgggatcgtc atcgaaaagt ctttccaaga tggacgtcag ttacttgaag
    19321 aaatgggatt gagagtcgtt tctcttgcgc gtattgcttc cttgacgaat ggacaagttg
    19381 agtttcttga ggaggacgca taatcgtgga aaaagaaaca caaaatggaa aagctgcggt
    19441 tttagggctg cagcatctgc tagcgatgta tgcaggagca gtagcggtgc ctcttttgat
    19501 cggtacagga ctaggcttta atcaagaaca gatgacttat ttgatttcaa ttgatatctt
    19561 tatgtgtgga ttagcgacat tattgcaatt gacagttaac cgatttttcg gaattggctt
    19621 gcctgtcgtt ttaggatgtg cgatccaagc agtagctcca ctgatcttga tcggaaccaa
    19681 tgaaggagta ggagctatct acggatctat tatcgcttct gggatctttg ttgtgttagt
    19741 ttctggtgtc ttttctaaaa ttaaaaaact attccctcct atagtaacag gaacggttat
    19801 caccgttatt ggtttgactt tgattcctgt agcaatcgag aaaatgggtg gaggtaatgc
    19861 aacagcagct gattttggtg acaaaacaaa tctgctactt gcatttgtga cgattctcct
    19921 aattgtaggc gtgcaaatgc tggcaaaagg gttcattcgt tcaatcgcgg tattgattgg
    19981 tttagtcgga ggaagcatct tagctgcttt catgggaatg attgatctga gcgcgattgg
    20041 agatgcgccg ttgttccatg ttccacaacc cttttatttt ggtaaaccaa ctttcgacgt
    20101 atggtcgatt ttactaatga tcatcatttc catggtcagc atggtagaat caacaggtgt
    20161 atattttgca cttggtgaca ttactgggaa aaatgttgga gaagaagaat tgaaaaaggg
    20221 atatcgtgca gaaggacttg ccgttatctt aggcggtatt ttcaatacat tcccttatac
    20281 tggcttttct caaaatgtcg gattggttca actttcggga atcaaaacaa gaaaacccat
    20341 ctatttctct gcattcttct tgattgtttt aggattattc cctaaaatcg gtgcgttagc
    20401 acaaatcatc cctgagccgg tacttggtgg aggaatgtta gtcatgttcg ggatggtggc
    20461 agtccaagga atccgtatgc tgttggaagt tgatttcagc aatgataaaa atctattgat
    20521 tgttgctgtt tctgttggat ttgggctggg tttcaatgtt acgccgacat tattcagtca
    20581 aatgccgcaa acattgcaaa tgttcactgg aaatggcatc gtaatgagta gcattacggc
    20641 tatcatattg aatttgatct ttaatggatt gaaaaaagat gaagaaatat tggaagaagc
    20701 cttcaagaag taatagagca aaagaaaggt tgtgaaatca gcgttttgac ctgattgtta
    20761 gagaaacaaa cggaaaaata gttcctcgta ttttgccgtt tgtttgagct aataccgagg
    20821 gtcagctgat tgaacaccgt ttatccataa aaaggaatgt gacctaactt ttgtcacatt
    20881 cctttttttc atctttaata aacacgaaca ataaaatgtt tttctttgtt aacattcgaa
    20941 atttctttga catcaaaaaa agtgattgtt aaaatatgaa tgtagaaaaa ctgggaggaa
    21001 aaacaatgac agcaaaaatc tctgtgatta tggggagtac ttcagattgg gaaacaatga
    21061 aagaagcatg tcaaatattg gatgaattcg gtgtgtctta tgaaaaaaag gtcgtttctg
    21121 ctcatcgaac cccagatttg atgtttgaat atgcacaaaa cgctcgaaaa aaaggaatca
    21181 aaatcattat tgcaggagca gggggagccg cgcatctgcc gggtatggtc gcatcaaaaa
    21241 caactttgcc ggttatcggt gtaccggtcc aatcacgaac attaaatggt ttggattcgc
    21301 ttctttcgat tgtccaaatg cctggaggag tacctgtagc tactacagca atcggaaaag
    21361 cgggagcaat taatgctgga ctacttgcga tacagatgct ttccatgtat gatttagaat
    21421 tggaggctaa attggctgaa agacgttcaa tgttggctaa aactgttata gaaagtagtg
    21481 atcaacttgg ttaaaccttt gttgcctggt tcaacgatcg ggatcgtcgg tggcggtcaa
    21541 ttaggtcgta tgatggcgat tagtgcaaaa gaaatgggct ttaaagtagg tgtgctggat
    21601 cctgtgagcg attgtccagc cgcacaagtt gcagattggc acattgaaag tgtctatgat
    21661 gacacctttg cgctgaaaga gcttgccaga agaacagatg tcattaccta tgaattcgaa
    21721 aacgtcagtg tagaggcgtt aaatgcgatt ttacccatgt catttattcc tcaagggaca
    21781 gacttgcttg cgattacgca agaccgcctg ttagaaaaat cttttttgga aacgaataac
    21841 atcgtgattg ctccttatgc aacgatcgtc agtccgacgg atatccaaga tgcgattgac
    21901 gggatcggct atccttgtgt tttgaaaacc actcgaggcg gctatgatgg caaggggcag
    21961 tatgttttga aaagtcgggc ggatctagct cctgccatgg accttttaag agaaggaacc
    22021 tgtgttttag aagcatggat cccgtttgaa aaagagattt cgatcatggt ggcaggaaat
    22081 ggtcaaggag atttcacgac ttttccggtc gtggaaaaca tccaccacaa caatattctt
    22141 catgaaacga ttgctccagc cgcgattgat caggatgtga tcgaggaggc tgagcgtatt
    22201 gcccgtgtga ttgcagaagc agtgagcctt tcaggtgttt taggggtaga aatgttttta
    22261 acaaaaacag gcgggctata tgtgaatgaa ttagctcctc gtcctcacaa ttctggacat
    22321 tattctattg aagcttgttc tatgagtcag ttcgatgccc atattcgagg tatttgtggt
    22381 tggccattag gagaaaaagt acgtttgata agcaatgcag taatggtcaa tgttttggga
    22441 gaacaactgc tggaaagcta tcgcttgatt gagaataagc cagactggca attccatttc
    22501 tatggaaaaa aacaagcaaa aaaaggtcgc aagatgggac atatcactat cttgacagaa
    22561 gacatccagc aaacgctgga agaaatcaaa caaacgaata tttgggatta gaggagaaaa
    22621 aacatgttag aacgttatac acgccctgaa atggggaata tctggacgga agagaacaaa
    22681 taccgtgcgt ggctcgaagt cgagatttta gcagatgaag cttgggcaga acttggtgaa
    22741 atcccgcaag aagatgtgaa aaaaattcgt gagcatgcat catttgatat cgatcggatc
    22801 ttggaaatcg aaaaattaac acgccatgat gtcgttgcgt ttacacgagc ggtttctgaa
    22861 acattgggag aagaaagaaa atgggtccat tatggtctaa caagtacgga tgtcgtagat
    22921 actgcttatg gctatctcat caagcaagca aatgatattt tacgggaaga cttgaaacgt
    22981 tttacggaaa tcattggcga aaaagcaaaa gaacacaaat atacagtgat gatgggtcgg
    23041 acacacggtg tccatgctga accaactact tttggattga aacttgccac atggtattca
    23101 gaaatgaaac ggaacatcga acgatttgaa catgcggcaa aaggagtaga agcaggaaaa
    23161 atctcaggag cagtgggaac gttcgcaaat atcccgccat ttattgaaga gtatgtatgt
    23221 aaacaattgg ggatccgtcc acaagaaatc tctactcaag ttctccctcg ggatctacat
    23281 gctgaatatt tctcagcgat ggcgcttatt gctacaagta ttgaacgttt tgcaacagag
    23341 atacgcggat tgcaaaaatc cgaaacacgt gaagtagaag aattttttgc gaaaggtcaa
    23401 aaaggttctt ctgctatgcc gcacaaaagg aatccgatcg gttcagaaaa tatggctggt
    23461 ctagccagag tggtgcgcgg acacatggtc actgcctatg aaaatgtcac attatggcat
    23521 gaaagagata tttctcattc ttcagcagaa cgaatcatct tgcctgatac cacgatttta
    23581 ttgaattaca tgttaaatcg ttttggcaat atcgttaaaa atctaacggt ctttcctgaa
    23641 aacatgaagc ggaatatgtc tgcaacattt ggtttgattt atagccagcg cgttatgctg
    23701 aaattgatcg ataagggaat gacaagagaa gaagcttatg atcttgttca gccaaaaact
    23761 gcgtacgctt gggacaacca agtgcagttc cgcccgctgt tagaagaaga tccaaccatc
    23821 acatcggtat tatctaaaga agaaatagac gatgcctttg attatcatca tcatttgaaa
    23881 aacgttgacg tgatattcaa acgagtaggt ttggaataaa gaagagtgag ttgcaaaatt
    23941 caattaaaat agaatagcaa aaataaccaa aaaagaagca gatccgttag cggttctgct
    24001 tcttttagtt tattatattt tcttatcaat aataggaacc tgattttaaa agcggctacc
    24061 tctttaccgt gcctctttac atacaaaaaa tagaggaatt aagcgtaaat ggcttggcta
    24121 ttataactaa aatgctggtt atggagatgt tgcagcaagt tttcgagaaa agcgtattcc
    24181 atctcttgtc cattgaataa gatctggata cgataatgat ttttgaaacg cttctgttca
    24241 tgaatctgac tgattgaaag ttcgcctttg tgttctgtct gataaagaaa agtcctttta
    24301 cggcattcag cacagcgctt ggttgctcgg cggatttttc ggaatgtata aaaactagta
    24361 tctttattgc ataaataaac gtctttgatc gacagcaaat cattatatag acaggaacgt
    24421 tcttcagctg ttaattctga taaaaaaagc tgaccttgtt ttattgcatc ttccatcggc
    24481 aagtgcctcc tcgaacccat tatccaaatt tttagttaat tctaatatac catcattaag
    24541 tataaatgta aacgcttgtt gatggtataa gattttcgtt tctgataaaa cacgaacatt
    24601 aaaagaatct aatgccttaa atgtacatat ttttgttgat ttataaaaag atgattgcta
    24661 aaatttaaag tgaaaaaacg aacaaaaaac gatgtatttg cttttaatgt aaataaaaag
    24721 gagagacgta catggagaag aaagaacttt tgtatgaagg aaaagccaaa aaaatatatc
    24781 ttacttctga tgagaacttg atttggatag aatatttaga tcaagcaact gcgttgaatg
    24841 gcgcgaggaa agaccagata agtggaaaag gagaattgaa taatcaaatc acaagtgcaa
    24901 ttttccgtta tctgactcaa agaggaataa aaaatcattt tattgaacaa ttgtctaaaa
    24961 ccgaacaatt agcaaaaaaa gtacaaatta tccctctgga agtggttatc cgaaatattg
    25021 cagcaggaag tttctcaaaa cgtctaggtg ttcccgaagg taaagaattc atcaaaccaa
    25081 tcattgaatt ctactataag gatgaccagt tggatgatcc atttatcaat gaagagcata
    25141 tctatttctt acaacttgca aaaccggaag aaatcagtga gctgaaagcc caagcgttgg
    25201 ctattaatga aattttgacc gaattatttg ctaaggtcgg tttggtttta gtagatttca
    25261 agctagaatt tggccggtcg aatggacaaa ttttgcttgc ggatgaagta acaccggata
    25321 cttgccgatt atgggatgaa cgtacgaatg aacatatgga caaagacgtt taccgcagag
    25381 atctaggtga tttgatccca gtctatcaag aagtatataa ccgtctatcg gaactaactg
    25441 aagaaaagga gcgagtatga tgtatttcgt aaaagtatat gtaacctata aagattcagt
    25501 gttagatcct caaggggagg cagtaaaaaa agcagtccat cgtatgggat atgaaacaat
    25561 cgaagatatc cgaataggaa aatactttga gatacaactt gctgatgcag aagaaccaat
    25621 cgatacagtg attgaaacga tttgtgacaa gctgcttgca aatgtaaata tggaaacata
    25681 ccgatatgaa atcaaaaaaa tggaggaagt ataacatgaa atttgcggtc atcgtttttc
    25741 caggttcaaa ttgtgacgca gatatgttat gggcagttcg cgagatcatg ggcgctgatg
    25801 ccgaatttgt acggcatgat gagtcctctt tagaaggatt tgatggggtt ttactgccgg
    25861 gaggattctc ctatggtgac tatttgagat gcggtgcgat tgctcggttt tctacaatca
    25921 tggaagaagt gattcgtttt gctgaagaag ggaaaccagt attcggtaca tgcaatggat
    25981 tccagatatt gacagaagca ggacttttgc caggtgtact gagacgaaat gagtctttgc
    26041 attttatttg caaaactgta ccattgaaag tcaaccccaa gacggccttt acttcagagt
    26101 atgaagccga cgaaataatc tatcttccag ttgctcatgg ggaaggaaat tatttctgtg
    26161 acgaagaaac acttcaagaa ctaaaaaaaa ataatcagat tgcctttact tataatgaca
    26221 acatcaacgg cagcgtggaa aacattgcag ggattacgaa caaagcaggg aatgtgttag
    26281 gaatgatgcc tcatccagaa cgtgcagtag aagacttgct aggatcagaa gatgggaaac
    26341 ggttctttgc ttccatttta aaaaacttcg gaaaggtgac cgtatagatg aaaacattga
    26401 aagaaccgac agctgaagag atcaagaaaa aaagaatcta tgccgaatgg ggattgactg
    26461 atgaagaata tcgaatgatc gaagaagata tcttaggaag aatgcctaat tatacggaaa
    26521 ctggactgtt ttctgttatg tggagcgaac attgttccta taaaaattct aaaccggttc
    26581 tacgcaaatt tcctactagt ggacctcatg tgttgcaagg ccctggagaa ggcgcaggca
    26641 tcgtcgacat cggtgatgga ctagcagttg tcttcaaagc agaaagtcac aatcatcctt
    26701 cagccgtgga gccttatgaa ggagctgcga caggagttgg cggaatcatc agagatattt
    26761 tcagcatggg ggctcgtcca attgcgttgc tagattcatt gaggttcggc gaattagata
    26821 atccaagaac gaaatattta ctagaagaag tagttgcagg tatcggcgga tacggcaatt
    26881 gtatcggcat accgactgta ggcggcgaaa ttgcatttga tccttgttat gaaggaaacc
    26941 cattagtcaa tgcaatgtgc gtaggtttga ttgaacacaa acatatccag aaagggcaag
    27001 ccgctggcgt cggtaattcg atcatgtatg taggtgcaaa aacaggtcgc gacggtatcc
    27061 acggagcaac ttttgcctca gaagaattta atcaagaaga agaacagcaa cgttctgctg
    27121 tacaagtagg cgatcctttt atggaaaagc tgctgcttga agcgtgtttg gaattaatct
    27181 tgcatcattc ggattctcta ataggtatac aggatatggg agcagcagga cttgtttcat
    27241 ctagtgcgga aatggcttca aaagctggct caggacttat attgacatta gatgaagtcc
    27301 cacaaagaga aactgggatg acaccttatg aaatgatgtt atcggaatca caagaacgga
    27361 tgttgatttg tgttaaatca ggagaggaag aaaaaatcca agaactattt cagaagtatg
    27421 atctagatgc agtcactatc ggtaaagtaa cagacgacgg gcaatatcgg ctatatcatc
    27481 aaggagaaga agtagcaaat ctgccagtcg atgcattagc tgaagatgca ccagtttatc
    27541 ataaagaaat gaaagaacca gctcgaatcg tagaatttca gcagttagct ccttatcagc
    27601 cagttatcga agaacctgga caagtattgc ttgatctgct gcagcaaccg acgattgctt
    27661 caaaacgttc aatctacgaa acttatgatt cacaagtaca aaccaataca gttgtccgtc
    27721 caggaagcga tgctgctgtg atgagggtta gagggacgaa taaagcgtta gctatgacca
    27781 cagactgcaa cgcgcgctat ctgtatttgg atccaaaaat cggaggacaa atagcagtgg
    27841 cagaagctgc tagaaatatt attgctagcg gtggtaagcc cttggcgatc accgactgcc
    27901 tgaactatgg ttctcctgat aaaccagaag ttttttggga attatcgact tcagctgacg
    27961 gaattgctgc tgcgtgtgaa acgttaggaa ctcctgttat ttcaggaaat gtctctttat
    28021 ataatgaaac ggatggtcaa gcaatctatc caacaccaat gattggtatg gtcggattga
    28081 ttgaggatca taaacatatc actactcagg aattcaaaaa atcaggggat ctaatctata
    28141 tattaggtaa gacgtttgca gattttgatg gcagtgaatt acaaaagatg cagcttggaa
    28201 gaatcgaagg tgtgatccga aactttgatc tatccattga aaagagaaac caagagttag
    28261 tattgacagc catccaaaac ggattgattg aaagtgctca tgactgttca gaaggtggct
    28321 tggcaattgc actggcagaa tcagctttca aacatcagct agggatatct gtacagtttg
    28381 aattgtcctc tgctcaatta tttgcagaaa ctcaatcaag atttgtgttg acagtcgcac
    28441 cggaaaataa gacacgattc gaagaaatga tgggagatgc tgctgtttta gcaggtaagg
    28501 tgacggatga agcgataatc gaaatttcag caactgacgg tcagatcaaa atagaaactg
    28561 cagtagcaag aaagtgctgg gaggatgcaa tcgtatgtct tatgaagtga agagtttaaa
    28621 tgaagaatgc gggatcttcg gtgtttgggg tcatccagac gccgctagtg tggcatactt
    28681 cgggctccat agtcttcagc atcgaggaca agaaggtgca ggtatcgtgt ccaatgatcg
    28741 aggaaagctt aaggggcatc gagatttggg gctgctttct gaggtattca aagatcaaag
    28801 aaaattgcag cagttaacag gagaagctgc aatcggacat gtacgttatg caacagcagg
    28861 caatggaagt gtggacaata ttcagccttt tctattcaaa tttcatgatc agtcgatcgg
    28921 cttagcgcat aatggtaact tgatcaatgc tcgttccctt agaaaagagt tagaggaaaa
    28981 tggggcgatc ttccattcta actccgatac ggagatattg atgcatttga tccgacgaag
    29041 tgaagaaaca gattttttaa ataaattaaa aagtgctttg aatgaagtca aaggtggctt
    29101 tgcctattta ttaatgacag aaacggcaat gattgccgct ttggacccaa atggttttcg
    29161 tccattagca atcggacaaa tgaaaaacgg agcatacgtg attgcctcgg aaacttgtgc
    29221 attagaagtt attggggcac gttttataag agatgtccag cctggtgaga tagtgatcat
    29281 caatgacgaa ggaatccaga ttgattgctt tacgaaggac acgcagcttt ctatttgttc
    29341 gatggaatat atctattttg ccagaccaga ttcaaacatc gcagggatca atgtgcatac
    29401 tgcacggaaa aatatgggac ggaatttagc gaaagaatcg ccagtaaaag cagatatggt
    29461 cgttggcgta cctaattctt ctttgtcagc cgcaagcggt tatgcggaag ctagtggaat
    29521 cccttatgaa atcggattag taaaaaatca atacattgcc cgaacattta tccagccaac
    29581 gcctgagcta agagagcaag gtgttcgaat gaaattatct gctgtacgag gagtagtgga
    29641 aggtaaaaag gtgattatgg tcgatgattc aattgttcgg ggtacgacaa gcagacggat
    29701 cgtccatctt ttgaaagaag caggggcaaa agaagtccat gtgaggattg cttctcctcc
    29761 tttgaaatat ccatgttttt acgggattga tattcaaacg cgtaaagaac tcattgcggc
    29821 aaatcacacg attgatgaaa tcaaagaatg tatcggtgca gattctttag gttttttaag
    29881 tgaagagggt ctgatcgaat cgatcggtct ggaaaaagat gctccgtatt caggattgtg
    29941 tatggcttat ttcaacggag attatccgac gccgttatat gactatgaag aaaaatatca
    30001 ggaatcacta aaagaaaaag tatcgttctt ttaaaagcag accaagaagg gagtatatga
    30061 attcaatggc aaatgcatac gcaaaagctg gcgtagatgt cgaagctgga tatgaagtag
    30121 tagaacggat caagaagcat gtcaaaaaaa cggaacgttt aggtatgatg ggcgctatag
    30181 gaggattcgg cggttgtttt gatttatcgg tctttcaatt aaaagagcca gtccttgtct
    30241 cggggaccga tggcgtgggg acgaaattag cagtggcaat cgaagcagac aaacacgata
    30301 caatcgggat cgactgcgta gcgatgtgtg tcaatgatat cttagcacaa ggcgcagagc
    30361 cgttatattt cttagactac ttagcaacgg ggaaaaatga tccgttacgt ttggaaaatg
    30421 tagtaaaagg tgtagcagat ggatgtgttc aaggaaatat ggcgctgatt ggcggagaaa
    30481 cagctgaaat gcctggtatg tactcaggta atgattatga tctagccgga tttgctgtag
    30541 ggatagctga aaaatctcaa ttattgacag gagaaacgat caacccagga gatgtgttgc
    30601 ttggaatagc atctagcggg attcattcaa atgggtactc tcttgttcgc aaaattttct
    30661 ttgagcagca gcagttttct ttatcatcaa aactgccaga attgaaaaaa acacttggcg
    30721 aagaattgct tgagccaacg aaaatctatg tttcttcctt gcttccgctg atcaaagaaa
    30781 agaaaataaa aggagctgca catatcactg gtggagggtt tgtagaaaat atcccgcgaa
    30841 tgttaccaga atctatggct gcacaaatca aacttggctc atggccgatt cttccaatat
    30901 tcggctgttt acaaaaatat ggtgaaattc ctgagatgga gatgtatgaa atcttcaata
    30961 tgggcatcgg tatggtcctg gcagtcgaag aatcgaaagt aaaagaaata gtacacgaaa
    31021 ttgagcaagc tggcgaaaaa gtttatgaaa tcgggaaagt gatcgccaaa gaaaaacatc
    31081 gtgtggtgtt tataggagaa gaacgatgag aatcgctgtt tttgcttctg gaaacggaag
    31141 caactttcaa gcgctagccg attatctatc gaaaaaagga ttggaatctt cgattgactg
    31201 gttgttttgt gatcagcctg aagcatatgt tttgaaacgt gcaactgcgt tgagtgtacc
    31261 ggcagattgt ttttcaccta aagaatttga ttcaaaaaaa gagtatgaag aagcaatttt
    31321 gcataaattg aaagaaaaaa agatcgattt gatcgtatta gctggatata tgcgaatcat
    31381 tggaccggta ttgctgaaaa attatgataa aagaatcatc aatatccatc cgtctctttt
    31441 acctgccttc cctggtttgc atggcatacg tgatgccttt gaagctggag taaaagaaac
    31501 gggtgtcaca attcattata tcgaccaagg tgtagatact ggaccaatca tccggcagga
    31561 aaaagtgaga attgaacagg aggacacgtt cgattcttta gaagaaaaaa tccatagagt
    31621 agagcatcga atctatccag aagtgattag tgaaattatt gaaaatggag gctatctgaa
    31681 atgacaagag cattgatcag tgtatcagat aaaactggga ttgtagcatt cgcccaaggt
    31741 ttgattgaac aaggagtaga aatcatttca acaggaggaa caaagcatgc gttggataaa
    31801 gctgggatca aaacgctttc gatcgaagaa gtgacagggt ttcctgaaat gatggatggc
    31861 cgtgtcaaaa cacttcatcc taaaatccat ggtggtcttt taggaagacg cgatctccct
    31921 agccatatgg aggcaatgga aacggaaaat atccaaccaa tcgactttgt ttgtgtcaat
    31981 ttatatcctt tcaaagaaac gattatgaaa ccagatgtcg aaatagcaga agctattgaa
    32041 aacattgata tcggtggtcc tagtatgctc cgtagtgcag caaaaaacca tgaatttgtc
    32101 acctctgttg ttgatccatc tgactatgaa gaagtacttt cagaattacg tcagtatggg
    32161 catacttcac tttctttcag acaaaaacta gcggcaaaag tctttcgtca tactgcggca
    32221 tatgatgcac tgatcgctca atacttaacg gattgggtgg aagaaaaaga accggaaaaa
    32281 ttgacactta cgtatgaacg aaaacaaacg cttcgttacg gtgaaaatag ccatcaagcc
    32341 gctgcctttt atcagtccgc tcttctggaa aaatattcga tcgcagcagc aaaacaactc
    32401 catgggaaag aactttctta taataatatc cgagatgcag atgctgcgct acggatcatg
    32461 aaggaatatg acgagccaac ggtagtcgcc ttgaaacaca tgaatccttg tggtattggg
    32521 acagcatcta cgattttaga agcgtgggaa gcagcgtatg aagcagatcc tgtttcaatc
    32581 tttggtggga tcatcgtttt gaatcgagaa gtagatctgc caacagcaga acagatgcat
    32641 aagctgtttc tagaaatcat cattgcccct tcttatacac aagaagcttt ggaattactg
    32701 caaaaaaaga aaaacatccg attactgaca gtagactttg cgcaggaaga aaatcaaaat
    32761 cgagaagaaa cggtctcagt cttaggtgga cttcttgtac aagatcaaga tctggcaata
    32821 gaaaatgaag aagagtggaa agtcgtgaca aaacgtaagc cgactccagc agaagcaaaa
    32881 gcattagcgt ttgcttggag agctgtcaaa catgtgaaat ctaatgcgat cgtactggca
    32941 aacgaacgtc agacagtagg aatcggagcc gggcagatga atcgagtcgg ttcagtaaaa
    33001 attgcgattg aacaagcgca agagaaaatg gaaggtgcag ttttagctag cgatgcttat
    33061 tttccaatgg atgattcggt tgaatatgca gccaaacatg gtataaaagc aatcattcag
    33121 ccaggaggaa gcatcaaaga ccaagattct attgatatgg cgaataaata cgatctagca
    33181 atggtcttta ctgatgttcg gcattttaga cattagagag gagtcggata aatgaacatt
    33241 ttggtgatcg gtgcaggtgg tcgtgaacat gcaataagta aaaaactttt agccagtccg
    33301 aaggtggata cggtgtattg tgcgccagga aatccgggga tgacaaaaga tggtatcaat
    33361 tgtgtcgcta tcagtgagcg agatcattca gcgttaatcc attttgttaa gaaagaggct
    33421 attgattgga gtatcgttgg tcctgaagtt cctttgttaa atgggatcgt cgatgatttt
    33481 atggcggaag gattgaaaat cttcggtccg acaaaagcag cagctctgat cgaaggttcg
    33541 aaaagttttg ctaaacaatt aatggatact tatcaaatcc cgacagctaa atccagaagt
    33601 tttgaatgtt acgaaaaagc agcagcgtat atcaaagaaa atggtgcgcc aatcgtagtg
    33661 aaagcggacg gcttagcagc tgggaaaggt gtagtcgtgg ctaccacagt gaaggaagcg
    33721 ctggatgcgg cagaacaaat gttgaaccag catcgatttg gggatagtag caaaaaaatc
    33781 gtcgtagaag agtttttggc aggagaagaa ttctcccttt tggcgtttgt ccgtaatacc
    33841 aaggtgtatc cgatggtcat ttcgcaagat cataagcggg catttgataa cgataaaggg
    33901 ccaaatacag gcggaatggg tgcttattca ccagtaccgc aaatttcaaa aaagatgatt
    33961 gaaaaagctg tggaagaagt tttaaaacca gcggctttgg gaatgaaaga actaggacgc
    34021 tctttcacgg gtgttttata tgcgggtttg attgctacga aggaaggacc aaaagtgatc
    34081 gaattcaatg cgcgtttcgg tgatcctgaa acgcaagtcg ttctttctcg gttgaagagt
    34141 gactttgcgc aagtaatcga tgatttatta gagaatcgta cagtagagtt aaagtggcag
    34201 caagaaggat acaatgtagg cgttgtggtt gcagctgccg gttaccccga agaatatgag
    34261 acaggaatgg ttctaccaga ttttctggaa gaagagcttt ctgtttacta tgctggagtt
    34321 tcctcaaata acggcaaact agttggagca ggaggacgtc tttatttagt agaagcatac
    34381 ggtgagacga tcaaagaagc acagaaaaaa atttacaccg cgttaatgga taaaacgaca
    34441 gatggaacat tttatcgtac agatattgga gaaaaagctg ccaatagcga actgatcgtg
    34501 aagtaaatca gctgcttcca tttataaaaa atacaacaaa cataatcgga agcgccgatt
    34561 ttagtgaagc cttttcataa agagtgtggt acacttttgt tgtgtatata aataaggagg
    34621 agttcagatg aaaaaaattc aattaggtac ttctgatcag aaagcagctg cagtgatctt
    34681 aggctgtatg cgtatcaaca atgcggaaga tccagtaaaa gtgattgaga cagcttatga
    34741 aaacggcatt gatttttttg accatgcaga tatttatgga aacggtgaat gtgaagaaat
    34801 ctttgcagat gctctagcaa aaacatcaat caaacgagaa gatcttttta ttcaaacaaa
    34861 atgtggaatc gttccaggtg tgatgtttga tttttctaaa aaacatatca ttgaatctgt
    34921 agaaaaaagt ttgacccgtc tgaaaatgga ttatgtggat gctttacttc ttcatcgtcc
    34981 agatacattg gtagaaccag aggaagtagc tgaagcgttt gatgaattgg aacggtcagg
    35041 aaaagttcgt tattttggcg tcagcaatca aaaaccaatg caaatcgaat tgctgaaaaa
    35101 atatgttcgt caaccattag tagctaatca gctgcaattt ggcttgaaac atactggaat
    35161 gatcgatcaa gggattcatg tcaatatgac agatgaagca agtatcgatc atgacggtag
    35221 tgtacttgat tattcccgat tgcatgatat gacgatccaa gcatggtcac cataccagta
    35281 tggatttttt gaaggtgtat ttatcgggaa cgaaaaattc cctgaattaa accaaacatt
    35341 agaagaacta gcagctaaat atcaaacgac accaactggc ttggcttctg cttggatttt
    35401 acgccatcca gccaatatgc aagtgatcgc cggtacgatg aacgtagggc gaatcaagga
    35461 aatcgctcaa gcttcagaaa ttaatatgtc tcgggaagat tggtacgaat tgtaccgtgc
    35521 tgctggaaac attttacctt aaagattgtc ataagcaggt caaaagggct ataaattaaa
    35581 aagacagaat aacttgaaag ctgttttcag cttcgagata ttctgtcttt ttttattgca
    35641 aatggaaaga gtatcattgt acggatttcg tttattagtt atttttgttt ttaaggaaaa
    35701 gctgtgtccg atattctttt ggagaaatcc cttgtttttt cttaaataaa taactgaaat
    35761 agttagggtc attataacca atttcaaaag caatatctga aatacgatag tctgattgct
    35821 gcaataattt ttttgctaat tcgattcttt gttctgtcag ataatcgatg aatgtttggt
    35881 taaacgattg agaaaaaatc gtactgaaat gagagggact taatgcaact tgttgagcga
    35941 cccaagttaa ggacatatct ggatcggtaa aattttcttt gataaaatcc aatgcatggt
    36001 tcaaaattgg ctgatatttg ttccttgaag gctgtgaggg cagagcagtt aagaaagaaa
    36061 tgaacatata aactgtcgaa cggtaaagcg agtcgtcggc gctgattgct aagcgttgac
    36121 tggtggatgt gcaatgaacc atttcttgcg ggattttctt ttctttcagg cagacatgct
    36181 ggagtagctt agtcaagaca aataaacgat ataatcgtgt tcgagttggt gtgccttgtg
    36241 gtttcgtcaa gtgttcgatg tacttagata cttcgttttt cgataatgaa gacaaatcaa
    36301 gtgagaggtc aaagtaaaag acagaatcaa ctggaagttt cgcttcttga atcaaatcct
    36361 catagctgat aatttgttct ttaagggaac tggcggtttc aagtatcatc tgcgtgatct
    36421 caaaagatgc aggtaattcg ctgattcttt ctgcgacagg accaatggcg acagaaaaat
    36481 tatttttttg tcccttttct aattcatgga ccaacgtttg ggctacctga taactggtct
    36541 cgatcacatt ttcttcaaga gaatcaacta ttaaaaattt gatgaatcga gaggaaacag
    36601 cagagaagat cacttgttga ttctccctaa ataaaaactt caaataatca ctgagatgag
    36661 cgtaatcttc aaagtctgta ctgacttggt ttgctgctaa taatacggtc atttttttcc
    36721 ctgctaaaga gcgattgaaa gattcagcaa gtttcaatgt ttcactgatc gtaagtgagc
    36781 ctttgaacaa accgttcaag aaatgatttt ttttcaattc gaaaacaaga tcagaagaag
    36841 tgtgcgtttc tttttgctgc aaatctaaag cagttactgc tttttctatt gcttgtttca
    36901 attcgctact tttaatcggt ttaagcagat aatcatctgc ctgtacctgg attgctcctt
    36961 tcatataatc gaaatcgtcg aatccactaa taaatatgag acgagtccaa ggaagtagtt
    37021 tccttgcttc ttttgaaaaa gtcaacccgt ccatgaaagg catttggata tcagtcagaa
    37081 tgatgtcggg ctgtaagtcg ataattgaag acagtgccat ctcaccatct ccagcttcgc
    37141 cgacataagt gattggataa taagaaccaa ggacagttat ttgttcccgc aagttattgc
    37201 gaatgaggta ttcgtcttcg atgataaaaa ctttatacat gtttgtttcc tccaattgtt
    37261 ttgggagata gagggatcat cagtgtgaca gttgttcctt ctcggtattt gctgtcaatc
    37321 gtgataggtt tttgatcatt tccgtaataa agaaggagac gcttgtttac gttgtaaagt
    37381 ccgtagccga tactgaaatc tgaatcgatc cctttggcta actctttacg tatttctgcc
    37441 agtttttctg cagggatgcc aatcccgttg tctataactt tgaattggat catctcatca
    37501 attttgcatg cggtaatacg aataaatcct ttgtgacgga cgtacttcgt tccatggtat
    37561 acagcatttt caatcaatgg ctgcaagatc atttttaaaa tagaaacagg aaaaagtgtt
    37621 tctggtatat cgatcttata gtcaagcata tggccgtatc ttatttttaa gatgactaga
    37681 taatcattga tatggctaat ttctttttca agaggaatcc agtcttgtcc tttgcttaaa
    37741 gacgtacgaa aaaagccgga aagtgcatgg gtcatagtca gagcttcttc aaagcagtct
    37801 tgttcgatca aagaaataat ggcatccaaa ctgttataaa taaaatgggg agtaatctga
    37861 gcttgcaata cacgaacttc actttgtgct aaatggtatt gtttcaaagc gttctcccgt
    37921 aaagactggt ttagatcttc cgccatttta ttcatgctag cggtaagtgt ttgtaattcc
    37981 agtgtttccg gcagggttaa acgaaagcca aggttccctg aagaaagttg ttctgacatc
    38041 tcgatcagct gattgatcgg ttcttggacc tttctctcta aaaagcgacg attctttttc
    38101 gcatagaaaa aaatggtact aagaactcca agttccacta ttgttaatag aatgagcgag
    38161 cggacaagtg tcgtattttt tttgcttgct aagtcgatct ccacacgaac aaaatcttgt
    38221 aagatatctg acagcagttg agtgacagaa tctacctgat tgatgatctc ttcattggta
    38281 tccataaaat cttttgccaa gatattattg atcagctcgt tttgataatc ttccagtgta
    38341 tcgatcgtac gtagcgatac atctaacgta ctaatttctt taagtgtatt tgtattattt
    38401 ttgatctctc gaatatcatt cctaagttct tctaggatat tttcctcatt gaactcatct
    38461 acggttactt ggccagaaat caagtcccac atactttcca aaactttttg atcaaccttc
    38521 aaagcaattg catctgcttt ttggatattt gcgatcgtct gttgataaat caaaagctgt
    38581 tgcgaatata acagtatgct cagtagcaaa gggataacag caaatagaag catggctttg
    38641 tttgcttggg caaaaatctg ttttatgctt aaattttttg tcatgatact tctccagtca
    38701 ataatttctg gttgggatgt tagctatatt ctgctgggaa aagacagtct cggaaataaa
    38761 aatatcttta ggtaatgttt ttcctgtttg aatatttcca aaataacgag taacggtatt
    38821 ggctacatac cagccgacat caggtttaca ctcgactaca caattgacga ttcctgagcg
    38881 tagcttctcg atcatatctt cttgtccatc aatagttaca atgatcaaat cttctccagg
    38941 tttgattgat gtttctttta tcgcagctaa agcaccgtga gtcatttcat cactatggct
    39001 gtataataca tcgatttcgg ataaatcatt tgtttctaaa tatcgtttca tctggtcttt
    39061 tcctttcatc cgaataaaat caccgacaat tcggtgaaca atagtcatcg gaggtttatg
    39121 gtcgatggtt ccatcttttt ttagggttgg tttacgattg attgattcca aaaatccttc
    39181 ggagcgtagc ttggtcggtg tggagttctc tgaaccagca agttctagga ttctgatttc
    39241 ctgtttcttt gaatttgtga agtgattgct gacaaataaa cccgcccgat ttccttctgc
    39301 tttgaaactg gggccaatgt gggtgacgaa aagctcggga tcattcgttt gaatatgtct
    39361 gtcgacaagt atgacaggga tccctgctcg ctttgcttct tttaatacag agtcccaccc
    39421 ggattcttgc aaaggagtaa aaatgatcat atccacttta taggcaatga acgtccgtat
    39481 atcttggatt tgccgttcct gattcatgta gccatttcgg tagagaactt ggtaatcttc
    39541 tttttcaagc gcagtgataa ttgattcagt atgctttttc cgccagctac tctccgttcc
    39601 tgattgcgaa aaaccaacga ctagtgggtc aggtgcttcc tttgtattag gtgtacaccc
    39661 catcatttgg taagcgatta caacaagggc taaaaaaaat aaaacacgtt tcactttaaa
    39721 tcactccatc tggatttgtt agtactatct tatcagataa aagggaaaat caaagtcaaa
    39781 aggaatcaat cgaaaaaaat ccaagaaact taaaaaaatc caagacaaaa aaagtacacc
    39841 taaaaaattt aaagtaaatc ccataaaaaa atcaatgtta acgctttctt tattagctat
    39901 actcggtgta tcaattgaaa tgggggataa aaaatgaaaa acaaatttag aaagattgtt
    39961 gggactatcg ctattttgac cgcaagcatt ctattaagtg cgtgtggcaa ttcaggatct
    40021 gcagatgata gtacggggta tgtaggaatc gctatgccga ccaaatcagc agaaagatgg
    40081 atcgctgatg ggaacaacat ggtctcggaa ttagaaaaac tgggttataa aacagatctt
    40141 caatatggag aagataaagt agaaaatcaa gtagcacaaa tagaaaatat gattacaaaa
    40201 ggggtagata cacttgtgat tgcttctatc gatggttcgg ctttaactga tgtgttagct
    40261 aaagcaaaag aagcagatat caaggtgatc gcctatgatc gtttactgat gaattcagaa
    40321 aatgtcgatt attacgcgac ttttgataat tttggagtag gtgtatcgca agcagcttat
    40381 attgaagagc acttaggatt gaaagaagga aaaggaccat tcacgattga attatttggt
    40441 ggctcgcctg acgacaacaa cgcactaatc aattacaacg gagtgatgtc tgttttacaa
    40501 ccatatatgg acaacggtca gttggtcgtg ccatctggac agacgagttt cagtcaaatc
    40561 gctacgcttc gctgggatgg ttctacggcg caagcaagga tggataatct gttaagcgca
    40621 aattatactg accaaacatt ggatgctgtt ttgtcgccat atgatccaat aagtttagga
    40681 atcatttcgt cattaaaagg cgtggggtat gggagtgaaa gtaaaccttt acctgtgatt
    40741 actggacaag atgcgacagt tgctggcgtt aaatcgatca ttgccgggga acaaacacaa
    40801 acgatcttta aagacacacg tatcttagca aaaaatacga ttgaaatgat caaagcaatc
    40861 agtgatggag aagaggttcc ggtcaatgat actgaaactt atgataatgg ggtgaaaact
    40921 gtccctacgt atttagcaaa tacagtatct gtcgataaag ataattatca agcagagctg
    40981 atcgacaccg attattataa agaatccgat ctgaaaaatt aagaggaacc aaacaaggaa
    41041 ctaaagagat ctaagtcaga gagaggataa gaattatggc ggattgtatt ttaaaaatgg
    41101 cagatatcac caaaaaattt tctggaatcc gagcgttaga ttgcgtgaat ctgatgattg
    41161 aacgtgggga aatccatgca ttgtgcggag aaaacggagc gggaaaatca acattgatga
    41221 atgtcttgag cggcttgtat ccatatggct cctatgacgg ggaaatcttt tatgatggaa
    41281 agctgtgtca atttaaagat ttgaaagata gtgaagaaaa aggcatcgtc atcattcatc
    41341 aagaattagc actaagtcct tatctttcaa taaaagagaa tatttttctc ggaaatgaac
    41401 aagcagaaag aggcgtgatt gattgggaac tgacagaaaa gaaaacatat gaactgctga
    41461 ataaagtcgg tttgtctatc gacccgaatc ttctagtatc ccagattggc gtagggcaac
    41521 agcaactagt tgaaatagct aaagcattat caaaatccgt ccgtctgttg attttggatg
    41581 agcctaccgc tgcattgaat gaagaagaaa gcgcgaattt gctgagattg atccgagagt
    41641 tgaaagagca aggtgtaacc tccatcatta tttcacataa gctgaatgaa atcgtggata
    41701 tcgcagatcg aatcaccatt ttacgggatg gacagacgat tgagacacta gaaaatgaaa
    41761 tgatcagcga agagcatatt attcgcggga tggtgggcag agatttagtt gatcgttatc
    41821 ctgaaagaaa accggatatt ggagaagttt attttaaagt agaagactgg acggtctatc
    41881 accctcttga tcacgagcga atcataaacg accacatcaa cttgcatttg aaaaaaggag
    41941 agattgtagg aatagctgga ctgatgggag cgggcagaac tgaattcgct atgagtctat
    42001 ttggacattc ttatggaagc aagatatccg gacgtatatt aaaggaaggg gcagagatat
    42061 cagttaagac cgttccttca gccattgcaa acggactcgc ttatgtctcg gaggaccgaa
    42121 aatctttagg actgaatctg ctgatggata ttcgggaaaa cgtgacgctg agcagtttgg
    42181 ataaaataag tcaaaaagga gtattagata aagaaaaaga agtaactgag gcagaaaatt
    42241 tccggaaaaa gatgcgtata aaaacaaact ccatccatca aattgtcagt agtcttagtg
    42301 gtgggaatca acaaaaagtc gttttatcca agtggctgat gacacaaccc gatattcttt
    42361 ttttggatga gccaacccgt gggatagatg ttggggcaaa atatgagatc tacacgatta
    42421 tcgaagaaat ggcagcaaat ggcaaagctg tctgtcttat ctcttctgaa cttcctgaaa
    42481 tattagggat gtgtgatcga atctatacga tgaacgaagg acgtattaca ggagaagtct
    42541 cgcgagaaga tgcagatcag gaagtcttga tgaaattaat gacgaaagaa gaggtgggtt
    42601 aagttggaac aggcaaaaaa tcttacggat aaaaaagaga aaaaactaaa tttgaaaagt
    42661 agagcagctg atctttttgg tcgctacagc atggtgatca ttttagcggg actacttatt
    42721 gcgtttcaat tgatgacgga tggtattttc tggcgtccat tgaatataac taaccttgtt
    42781 ctgcaaaata gccatatatt agtactagct gcagggatgt tattagttgt gctcttaggt
    42841 catgtggatt tgtcggtagg gtcagtgatg gcgtttgttg gtgcaattgc gggcatgatg
    42901 atggtcaatc gccaaatgaa cccgtggatg acagttatcg catgtcttgc agtaggcggg
    42961 ctgatcggcg catggcaagg tttttgggta gcttatgcag ggataccggc gtttatcgtg
    43021 actttagcag gattgctgat gtttcgtgga ctgacacaag ttgttctagg aggccaatct
    43081 ctagcacctt ttccagctgt tttccaaaaa atgtctacgg gttatttacc agacttaagc
    43141 gaaggaagca gccttcactt cttgacattg attttaggaa tgatcatcac gatcgtgtta
    43201 gttgttggtc aatggcgaac aagaaatcgt cggaaaaatc atttatttga agtggaatct
    43261 ccaacagctt tcttggtaaa ggcggtattt acagcagtcg ttgtactagg aataacttat
    43321 atctttgcct cttatcaagg gtatccgatt attttgatca ttcttggttt gatcgttgca
    43381 gcatatgggt ttttgacaaa taaaacagta gcaggtcgac agatttatgc gacgggcgga
    43441 aatcgaaaag cggctgaact atcagggatc aaaacaaagc gaatcacgtt ctgggtattc
    43501 gtcaacatgg gagtcatggc agctttagct ggattagttt tagctgctcg attgaatgcg
    43561 gcaacacctc aagcaggtac atcgatggaa ctggatgcaa tggcggcagt atattttggt
    43621 ggtgcttcga cttccggcgg cattggaaca atcatgggtg cgattgtcgg tggcttggtc
    43681 atgggtgtat tgaacaatgg catgtcgatt ttaggcgtcg gggtagactg gcagcaggca
    43741 atcaaaggat tgatcttgct tttagccgtt gtgctagact tgtataataa gaaaaaacga
    43801 acaacgtagg agaacgccgg ttattcagaa aaaagactgt gacaagacat ttgtcacagt
    43861 ctttttttcg ctaaaagcga acattcattg tttcgtttgt atagactaaa aataatatat
    43921 gtttttttac cattcgtata aattctcttt ttgctcttga tttttttgct taattgagtt
    43981 aaattacata gggcgctaaa aaaagccttg attaaattaa taatgttcgt gttttgctgg
    44041 atgtgaacat caggaggata atgtaatgaa agtatttgat tacgaagata ttcaattaat
    44101 tcctaataaa tgtatcgtaa atagccgttc tgaatgtgat acaacggtca cattaggtaa
    44161 acatacattc aaaatgccag tagtgcctgc caacatgcag acgattattg atgaaacaat
    44221 cgcagaattc ttagcggaaa atggttattt ctatatcatg catcggtttg atgaagcagc
    44281 tcgaattccg tttatcaaaa aaatgaaaaa gcgtggattg attacctcga tcagtgttgg
    44341 cgtcaagaaa gaggaatatt cctttataga gaagttggca gaagaatcgc taaatcctga
    44401 ttatatcact atcgacatcg cgcatggaca tgctaactca gttattgata tgattcagca
    44461 tattaaaaaa tatttgcctg aaacttttgt gattgccggt aatgttggta caccggaagc
    44521 cgtccgtgaa ttggaaaatg cgggcgcaga tgcaacaaaa gtcgggattg gtccaggaaa
    44581 agtatgtatt acaaaaatca agacaggctt tgggactgga ggctggcaac tggcagctct
    44641 tagatggtgt gcgaaagcag ctcgcaagcc gatcatcgct gacggtggga tcagaacaca
    44701 tggcgatatt gcaaaatctg tccgttttgg tgcgacaatg gtcatgattg gttcattgtt
    44761 tgccggtcac gaggaatctc ctggcgaaac aaaagtcgaa aatggaatcg tgtataaaga
    44821 atactttggt tctgcttcag aattccaaaa aggcgaaaaa cgaaatgttg aaggaaagaa
    44881 aatctggatc caacacaaag gatctttgaa agatacacta gtggaaatgc aacaagacct
    44941 tcaatcatct atttcttatg ctggaggaag agatctggaa gccattcgaa aagttgacta
    45001 tgtcatcgtg aaaaattcga tttttaatgg agacacaatc taaaaaataa ataaaatatt
    45061 aggatgttgt gaaaaagttg tcctgcatta agtaataaga acagccaaac aaaaatagtt
    45121 ctttatatgt tttgtttggc tggaattatt atcgcagata caagcttttg aacatcatta
    45181 ttcagaataa gggactgtga taagattttt gtcacagtcc cttatgtttt gcaaaaaaag
    45241 tttttctttt ttctcgtaaa ttcggtaaaa tcaaaagaaa aaatagacaa tagaaaagag
    45301 aagcttatgt gggaaaaagt agagaaaaaa caaggaccga tttatcgtca aattatggaa
    45361 cagattatga gagaaatcga aaacggaagg atcaatcctg gaagtcaact tgaatcagaa
    45421 cgaaaactag ggcaactttt tggtgtgaat cgttccaccg ttgtccatgc attggaagag
    45481 cttagggagc ttggggtact aacgagcaaa cgtggcagcg gatggtttgt taatcagact
    45541 gaatggggga aattcgctgt tccaagaatc gattggcggc aaatgatctc tcctcgttat
    45601 gagcagacgg actggtatga gcaaaaaata agagaagcaa agcagatcca aaaaacatcg
    45661 tttcttgatt tatacgctag cgagatgcca gatgagcttt tgccgaattt tgagtttcct
    45721 tcttattctc ttgaagaaat cttagcggaa gaaaaagaaa tggatctttt aggctatact
    45781 ccgttacgta acaaaatcaa acggtatttg gaaagtaaat ttgaattggc attttccccc
    45841 aaccagcttc ttgtgactgg tggcggtcag caagcaatct ttttgatttt acaaacgatt
    45901 ctttcagctg gagaggggat agctgtagag tcaccttctt ttttttaccg attacctctt
    45961 ttcaaagctt caggaacgcg tttatttggg ataccgatgg atgaagaagg gatcgactta
    46021 gaaaaactga aagaatcgat ccataaaaat aaattgaaag cagtgcttat caacccgaat
    46081 tttcaaaacc ctacaggaaa agtgatgagt caaaaacgac gagaaaagct agtagaattg
    46141 tgtcgacagt atcggcttcc aattattgaa gacgatgtat ttacggatct ttcatttcta
    46201 aaccaagaag aagtccttcc tatacgaacg attgatccgg ataatgtgtt atacgtcggt
    46261 tcgttatcta gggttttagg aaaaacaacg aaaatcggtt gggtgatagg acctgagact
    46321 tttatctcgc aactagctca agcgcaagaa atgatggagt tttcgatgaa tatccttaca
    46381 caaatcacag tggctaatgt atttaatgaa gcaattgatt tgaaaatgca agatgtaaga
    46441 aaaaagttga aagaaaaaag ccgactgata agcgcttggg gagattcaca atcgtttttc
    46501 aagcttcatt caattttagg tggttactat gcctggatta cttgggatgg gaaaaaacta
    46561 gaccccgaac tagcagaaaa agtagtagat atgggtcttg gaattgcacc aagtctactt
    46621 tttgggcaag atataaatgg aatcagaatc aattacagta ggctggatga aaaccaaata
    46681 ccgttgtttt attcaaagat gaaaattcta gaaaagtggt taacagaata atttcacaaa
    46741 gtaaatagat taaagaatat aacagcttgg atttaaattt caagctgttt cttttttttc
    46801 ggaaaaagaa tacgatataa agaaactaag aaaaaaccat tatttgtacg tccgttcacg
    46861 atgtgtaaat aaaataaatg tttgcaacaa aactgtataa caccaacgtt tttaaataga
    46921 aacagatggt tgtaagtttg attagaaaat agaaaaaatg cagatgaaaa ataaaacgct
    46981 ttccttattg tgattttatt cactaatgct gttaatatgc ggattggcat aatttccttt
    47041 catcgtgaaa atttgtgctt ttagcttgta tttctcataa atccatgata atttattaat
    47101 gaagacggtt aaatagtgat acagttttat tcactgtttc aatcgcagaa tgaagagatt
    47161 ttcttataga tgagagagga atgtgtgaca aatggcaaac aaaaaagcac cgatcgactt
    47221 ccaagcactg ctagatgaag tcaatgcaga tttcccagta tatcaagcac ttgatcaaga
    47281 tggaaacgta gttaacaaag atttagtacc tgatttatcc gatgatgaac tcgttgaatt
    47341 aatgactcat atggtatggt ctcgtgtatt agaccaacgt tcaacagcgc tgaaccgcca
    47401 aggacgccta ggcttttttg caccaactgc tggacaagag gcaagtcagc tggctagtgc
    47461 ttacgcattt gataaagaag atgttcttct tccaggttat cgggatgtac ctcaactagt
    47521 aaaacatggt ttacctttat ctcaagcttt tttatggtct cgaggacatg ctgcaggtaa
    47581 ttcttatccg gaagaattga aagctctacc accacaaatt atcatcggtg cacaatatgt
    47641 acaagcagcc ggtgttgcat taggtttgaa gaaacgaaac aagaaaaatg tcgtcttcac
    47701 ttatactggt gatggcggtt catcacaagg tgatttttac gaagcaatca atttcgctgg
    47761 cgcttaccat gcaaatgccg tattttatat ccaaaacaac gggtatgcga tctctacacc
    47821 tcgtgaaaaa caaactgcag caaaaacact tgcacaaaaa gctgtcgcag ctgggattcc
    47881 tggtattcaa gtcgatggaa tggatccgct tgcagtatat actgtatcta aaatggcacg
    47941 ggaatgggcg atcagtggaa atggaccagt cctaatcgaa acactgactt atcgttatgg
    48001 accacataca ctatctggtg acgatccaac ccgctaccgt acaaaagaca cagatgacga
    48061 ttggcagaaa aaagatccat tgatccgctt ccgtaaatat ttgacggaaa aaggactttg
    48121 gtctgaagaa aaagaagaac aagtcatcga agcaactaaa gaagaaatca aagcagctat
    48181 tgctgaagcc gacaaagtac caaaacaaaa ggtttcagat ttcttgaaaa acatgttcga
    48241 agtatctcca caatcaatca aagaacaaat tgcaatttat gaagcgaagg agtcgaaata
    48301 atcatggcac aaaaaacaat gatccaagca atcacagatg ccttagcact tgaactagaa
    48361 aatgacgaga atgtcgttgt ttttggtgaa gatgttggga aaaacggagg agttttccgg
    48421 gcaactgaag gattgcagga aaagttcgga gaagaccgag tattcgatac tcctttagct
    48481 gaatctggaa tcgcaggatt gagtttcggt cttgcgttag aaggatttcg tccagttcct
    48541 gaaatccaat tctttggttt tattttcgaa gcaatggacg aagtagtagc tcaaatggct
    48601 cgtacaagat atcgaatgag cggcactaga aatctaccca tcacaattcg ttcgccattt
    48661 ggtggcggtg ttcatacacc agaattgcat tcggataatt tagaaggttt gatcgctcag
    48721 tctcctggta ttcgagtagt gattccttcc aacccttatg atgcaaaagg actattgatt
    48781 tcttctatta gaagtaacga tccggttgtt ttcctagaac acatgaaact atatcgctca
    48841 ttccgagaag aagtgccgga tgaagcttac gaagttcctt tggataaagc agcagttact
    48901 cgtgagggaa cagatgtatc tattatcaca tacggagcaa tggtgcgtga agcaatcaaa
    48961 gcagcagaca atcttgcaaa agaaaatata tctgttgaaa tcattgatct tcgtacagtt
    49021 gcgccattag atgtagaaac gatcattcaa tccgtggaaa aaactggtcg cgtagttgtt
    49081 gtccaagaag cacaacgaca agcgggcgtt gccgcgcaag tagtttctga gatctcagaa
    49141 cgcgctatcc tttcattaga agccccaatc ggacgagttt ctgcacctga tacagtcttc
    49201 ccatttggac aagcagaaaa tgtctggttg ccaaatgcaa aagatatcga agacaaagta
    49261 aaagaaatcg cggaatttta agaaaagaag ggacgaaaca aagtcatggc ttatcaattt
    49321 aaattgccgg atatcggtga aggaatcgca gaaggtgaaa tcgtcaaatg gttcgtcaaa
    49381 ccaggagata cgatcaatga agatgataca ttactagaag ttcaaaatga caaatcagta
    49441 gaagaaatcc cgtcacctgt tacaggaacg gttaaaaatg tgattgttcc agaaggtacc
    49501 gtagcaaatg ttggggatgt attagtagaa atcgatgcac caggacacga agacaatgaa
    49561 ggggacagtg gtgtagccgc agaatcccaa actccagcta aaccggctgc tgaacccact
    49621 gttgatacag aatcagctgg atcgtcaagc gaaggagtct tccaatttaa gttaccggat
    49681 atcggtgaag gaatcgcaga aggtgaaatc gtcaaatggt tcgtcaaacc aggagatacg
    49741 atcaatgaag atgatacatt actagaagtt caaaatgaca aatcagtaga agaaattccg
    49801 tcacctgtca cagggacagt taaaaacgtg attgttccag aaggcactgt agcaaatgtt
    49861 ggggatgtat tagtagaaat cgatgctcct ggacacaata gtgcaccgtc aactagtgcg
    49921 ccatcagctg aagcaccaaa agaaaaagtg gaaacatcag gttcagcaag tgtcgttgaa
    49981 gctgccgatc caaataaacg tgtattggct atgccatctg ttcgtcaatt tgcccgtgaa
    50041 aaagatgtag atatctcaca agtaactgca actggaaaag gtggcagagt aacaaaagag
    50101 gacatcgaaa acttcttggc aggtggtcca agcagtgcac cagcgaaatc agaagcgcca
    50161 gaagcggcag caccgaaaga agcagctcct gcagcagaaa gcaaaccagc tgcaccagca
    50221 aaaccattca agtccaactt aggagacttg gaagaacgtg tagcaatgac gccaacacgt
    50281 aaagctatcg ctaaagcaat ggtcaacagt aagcatacag ctccacacgt gacattgcat
    50341 gatgaagtag aagtgtctaa actatgggat aatcggaaac gatttaaaga agttgctgct
    50401 gcaaatggta cgaaacttac tttcttgcca tatgtcgtga aagctttaac agcgactgtt
    50461 aagaaatacc cagtattgaa tgcatctatc gacgatgcaa accaagaaat cgtttataaa
    50521 cattactaca atatcggtat tgcaactgat acggatcacg gactatatgt accaaatgtg
    50581 aaagatgcag accgcaaagg aatgtttgct attgcagacg aaatcaatga gaaagcaaaa
    50641 ttagctcatg acggcaaact atctgcagaa gatatgcgca atggaacaat tacgatcagc
    50701 aatatcggat ctgtaggtgg cggttggttt acaccagtca tcaactatcc agaagtagct
    50761 attttaggtg taggaacgat tgcacaacaa ccaatcgtaa atgctgaagg cgaaatcgtt
    50821 gtcggtcgtg tgatgaaact gtcattaagt ttcgatcacc gtatcgtaga tggagcaact
    50881 gcgcaacaag caatgaataa tatcaaacgt cttttagctg atccagagct attaatgatg
    50941 gaaggatgat aaacaaatgg tagttggaga ttttgctgta gaattagata cagttgtaat
    51001 tggtgcggga cctggtggct atgtagctgc catccgcgct gctgaaatgg gacaaaaagt
    51061 agcaatcatt gaaagagagt atattggcgg tgtttgtttg aatgtcggct gtattccttc
    51121 aaaagcactg atagctgcag gacaccatta tcaagagtca ctagattctg aaatgtttgg
    51181 agtaactagt gaaaacgtca aactagattt tgcaaaaaca caagaatgga aagaaaacaa
    51241 agttgtccat acgttgactt ctggtgttgg tttcctatta aaaaaacata aggttgaaac
    51301 aatcgaagga gaagcgttct tcgtagatga tcacacacta cgcgtcattc atccagattc
    51361 agctcaaact tattcgttca ataacgcaat catcgcaaca ggttcccgtc caatcgagat
    51421 tccaggattt aaattcggag gacgtgtgtt agattctact ggcggattag cattgaaaga
    51481 agtacctaag aaatttgtga tcatcggtgg cggtgtgatt ggtgccgaat tgggcggagc
    51541 gtacgcaaac ttaggagcag aagtaacgat tcttgaaggc tcaccacaaa ttttaccaac
    51601 ttacgaaaaa gatctagtaa aattagtaga agatgatttc aagaaaaaag gcgtaacagt
    51661 cgtaacgaat gcaatggcga aagaagcagt cgataacggt gacagcgtga cagtgaaata
    51721 tgcagtggac gggaaagaag aatccgttac tgctgattat gtgatggtca ctgtaggtcg
    51781 tcgtccgaat acagatgata tgggcttaga acaagcgggt gtcgaagtag gagaacgagg
    51841 attgatcacg gtggataaac aaggacgtac gaacgtacct aacatctatg ccatcggtga
    51901 tatcgttcca ggggcggcat tagcacacaa agccagttat gaagcaaaaa tcgctgctga
    51961 agcaatctct ggcaaaaaag tagctgtaga ttataaagca atgccggcag tagccttcac
    52021 tgatcctgaa ttggcttctg taggtatgac gatcaaagaa gcaaaagatg ctggtatcga
    52081 agcaaaagca tataaattcc cattttcagg gaacggtcgg gcattatcgc ttggtaaaac
    52141 agaaggattc atccgtctag ttacgacgat cgaagataac gtgcttattg gcgcgcaaat
    52201 cggtggagtc ggcgcaagtg atatggtctc tgaattagct ttagcaatcg aatcaggaat
    52261 gaatgctgaa gatatcgcct tgacgattca tcctcatcca tctcttggcg aaatcgtcat
    52321 ggatgcttcg gaattagcat taggtttacc aatccatatt tgatttgaat aatacaggcg
    52381 aaacgcaact gaaaaatcag ttgcgttttg ttttttagaa ttatcaataa tattattatg
    52441 taaacttaaa aatatgacaa cttcttggtt tattaccact tcctatgcta cactgaagag
    52501 gaatatgaaa taggagagtt gaagatgcaa gaatatcagt atccacttga tttagattgg
    52561 acaacagacg aaatggtcat tgtaatgaag atgtgggaag atctggaacg tgcgaatgaa
    52621 aaagggataa acaatgaagc ttttttgaag acttatcagc agtttaaaac tgttgtcaaa
    52681 tccattggtg aggaaaaacg attgggacgg gaatttgaaa aagcttccgg ttattcttta
    52741 tatcgtacag tgcagcaagc aaaaaaaaat ccaaacaaaa ctttgagaat gaacgggtga
    52801 gataaatgga acagatgatt gatcatatca agtcctggct tgaagaagct ggtgagaaaa
    52861 tcaaatcttc tcaacaaaca ttaacaatct cgcaaaaatc aaatcgaacg gatctagtaa
    52921 caaatgtcga tcgaaacatc cagaattttc taatcgaaaa aatccattcc tttgatccag
    52981 aggcgaaaat attggcagaa gaagaagggt gtaatcaatt atcggatctc gatggaagag
    53041 tctttatcat cgatcctatt gatggcacct tgaattttgt gattgaaggg gaaaattttt
    53101 gtattatgct cgctgtttat gaatccggcg ttggaaagct tggtttcatt tatgacgtga
    53161 tgagaaatga acttttttgg ggtggaaaag gaataggcgt gtataaaaat gatgagccat
    53221 tgcctagacc agcaaataaa tccttatcgg aaggcttact tggtgtgaat agttatcttt
    53281 tcggtcataa taaatatgga atccgagaaa ttgcggaaag cagcatgggg acacgtatgt
    53341 acggatgcgc tggtcttgaa ctagtggcaa tcttgaaagg aaaccacatt gcttatattt
    53401 caaaccttag cccttgggat tatgcagcgg gaaatgtctt attggaagag tttggtatgg
    53461 taacaagcgg tctttttggt actcctttga catttgaaaa aagggaatac tatttagcag
    53521 ggacttcttg tgcatatgca gaaatcgcag accttttgaa aaaagaaggc tgaaaaaaag
    53581 gtagaaatca ttcacaaaaa tgataaaaac gagcaaaaga ttaattttct cgaaaaaatg
    53641 aaaacatttt actatatttc atgaaaaaag tctgctataa ttagcagtcg agtaaaattt
    53701 gccctgtcat aaagaaccaa taaaaaagga gttagttaca ttgaattata gaaacgatat
    53761 ccgtaacgtc gcaattattg cccacgttga ccacggaaaa acaacattag ttgatgaact
    53821 attaaaacaa tcagatacat tagacgcaca tacgcaattg caagaacgtg cgatggactc
    53881 aaatgcgcta gaaaaagaac gtggtatcac gattttagcc aaaaacacag ctgtagacta
    53941 taaaggtatt cgcgtaaaca tcatggatac tccaggacac gcggacttcg gtggcgaagt
    54001 agaacggatc atgaaaatgg ttgacggcgt tgtgttggtc gtagatgcgt atgaaggaac
    54061 aatgcctcaa acacgttttg tattgaaaaa agcactggaa caacatatca caccaatcgt
    54121 tgtcgtaaac aagattgaca aaccttctgc tcgtcctgag catgtcgtag atgaagttct
    54181 tgaactattc atcgaattag gagcagatga tgatcagtta gactttcctg tgatttatgc
    54241 atctgcacta aatggtacgt caagcttgtc agatgatcca gcagatcaag aaccaaccat
    54301 ggcacctatt tttgatacaa ttattgaaaa aatccctgct cctgtcgata acagcgacga
    54361 accattacaa ttccaagttt cattacttga ctacaacgat tatgtaggtc gtatcggaat
    54421 cggacgtgta tttagaggaa caatcaaagt aggagaccaa gtagcattaa ttaaattaga
    54481 cggaacagtt aagaaattcc gtgtaacgaa attatttggt ttctttggac taaaacgtct
    54541 agagattcaa gaagcaaaag ctggagactt gattgctgtc tcaggtatgg aagatatctt
    54601 tgtcggtgaa acagttactc ctgtggatca tcaagatgcg ttacctatcc ttcacattga
    54661 cgaaccaaca ttgcaaatga ctttcttagt taataattca ccatttgccg gtcgagaagg
    54721 taaattcgta accgctcgta agattgaaga acgtttgatg gcagaacttc aaacagatgt
    54781 atccttacga gttgaaccaa caaactctcc agatgcatgg acagtttctg gtcgtggaga
    54841 attgcatttg tctatcttga ttgaaaacat gcgccgtgag ggatacgaat tgcaagtatc
    54901 tcgtccagaa gttattgaaa aagaaatcga cggtgtaaaa tgcgaaccgt ttgaacgcgt
    54961 gcaaatcgat acaccagaag aatatatggg tagtgttatc gagtccttga gcttacgtaa
    55021 aggtgaaatg caagatatgg tccatacagg aaacggacaa atccgtttga cattcttaac
    55081 acctgcccgc ggtttgattg gttattcaac tgaattcctt tcaatgacac gtggttacgg
    55141 aatcatgaac catacttttg accaatatct gccaatgctt cctggtcaaa ttggcggacg
    55201 tcaccaaggg gcgcttgttt cgatcgatac aggaaaagca acgacttatt ctatcatgag
    55261 tatcgaagaa cgcggtacag tcttcgtaga accaggtaca gaagtatatg aaggaatgat
    55321 tatcggggaa aacagccgtg ataatgattt gactgtcaac atcacgaaag caaaacaaat
    55381 gacaaacgta cggtctgcta ctaaagacca aacatctgtc atcaaaaaac caaaacaatt
    55441 gacgttggaa gaatctttgg aattcttgaa tgatgatgaa tattgcgaag taacaccaga
    55501 aagcattcgc ttgagaaaac aaattttaga gaaaaatgct cgtgaaaaag catctaaaaa
    55561 gaaaaaataa tctgtaaaag gatgaagaga gaaaccagct tgttataaaa aatagctggt
    55621 ttcttttttt gtccaactct tttctgtgga gaagtcatct ttgtcaatgg acataaattc
    55681 agaaaatacg ccgaaagacg tttaaaagac ttttaaaaaa cttatttcta gagagttttt
    55741 gtttcataat aagataatgt ttcacgaaag gagatcaact tgtccttcga agaaaatcga
    55801 agtaaaatgg caagagaaag gagcgggaag atgaacgact tattaaaaca atactttggt
    55861 tatgatgagt ttagacctgg tcaaaaagaa attatccaga aagtcattga tcaggaaaat
    55921 gtattaggaa tcatgcctac cggaagtggg aaatcaatct gttaccagct tccagcttta
    55981 ctattagatg ggcttactgt ggttgtgtcc cctttaatct ctttgatgaa ggatcaggtg
    56041 gacgcggcga atcaattagg tatacccgcc acttttatca atagctcgtt agatggttac
    56101 gaaacagcta gacgcttcca agaaatcgat agacagcagt accgtttact ttatatcgct
    56161 cctgaacgtt ttatcatgcc ggattttatc caagcaatga agcgatggaa cgtccgtatg
    56221 atcgctattg acgaggcaca ttgtatctct cagtgggggc atgatttccg cccaagttat
    56281 ttacagatgg cgaaccagct agatcaactg ccaaatcgtc ctgtaattgt tgctttgaca
    56341 gctacagcga ctgtgcaagt agctgctgac ataaaaagac ttttaaaaat tccagaaaac
    56401 aaccatattc agactggttt tgaacgagaa aatctacgat ttcaagtaat taaagatcaa
    56461 aaaaaagaac aatatctaat cgaatattta aagatcaaca aaaatcaatc tggtatcatt
    56521 tatgcagcaa cgagaaaaga agtagaccgt ctctatcatt tgcttaaaaa attcgatttt
    56581 tccgtgggta gatatcatgg tggtctaaat gagaatgagc gaacagaaat gcaagaagcc
    56641 tttttatacg atcgcttgca gctgattgta gcgacaaatg catttggtat gggtataaat
    56701 aaaagcaatg tccgttttgt tatccattat caaattcctg gctctctaga agcatactat
    56761 caggaagctg gtcgggcggg aagagatggt ctttccagtg aagccatcct gcttttctct
    56821 cctcaagata tccaagtcca aaagtttttt atccagcagt cccaaagaga agaagggcaa
    56881 aagcaaaaag aatatgaaaa attaaaagcg atgacagaat acgtgtatat tgagtcctgt
    56941 cttcagcaat acatcctaaa ctattttggc gagacaagca gtccgtgcaa ccgttgtggt
    57001 aactgtttag atgacagaga aatagtagaa gtaacgacag aagcacagat ggttctttct
    57061 tgcttaaaac gtatgggtga gaactatggt aaacaaatgc tgatgaaagt cttagcagga
    57121 tcgaaagaac aaaagctgca agctttaggt tttggacatt tatctaccta tggactgatg
    57181 aaaaaccaat cgcaaaaaga aacgatgcag ttaattgaat atttgatatc gaatggctat
    57241 cttctgacca tcaatggtga gtatcctgtt ttgaaagtaa cagaaagagg gatacaagta
    57301 ttgaaaggac aagaatctgt ttatcgaaag gaacccaaga aagtccagca gctatcggat
    57361 gaagaaaccg atacactatt tgaagtgctg cgggaactgc gaacggatct agcatcagaa
    57421 gcaggcgttc ccccgtatgt cgtattttca gattccacat tgaaagaaat gagccgaata
    57481 cgtccatcaa gcagattgga gatgcttcag ataaaaggag ttggccaaag caagcttgat
    57541 aaatatggcg aagccttttt atctcggata aaaaatgcgg actctaacgt tctggataag
    57601 tagaaagaac gaatacgtaa aaatgaataa aaacaactgg ttaaatctgt tttttttgct
    57661 ccttatgaaa atcaaatatg ttataattaa ggaattagaa tgggaggtaa gcacagatgg
    57721 attctttgtc gaaaaaactt gcgatccaag tattaaatga tgaagcagaa cggatcaaac
    57781 gattaatccg aaatcaaaac aacagtctct gtatctcaca atgcaaagcc ttcgaagaag
    57841 tggtagatac acagatgtac ggcttttccc ggcaagtcgg ttttgctgtc cgtataggag
    57901 taatcggaaa gaacgagggc cagctgatgt taagtgagct agaacgagaa ttgaatcagt
    57961 tatatagtga ggtttaccaa gaaatttatg ataaaaaaga agttggcaag gaggaataga
    58021 cttgcctaag aaagtgaaaa aaaggcatct attagactat agcatcttga ttccctattt
    58081 aattttatgt gtattgggat tgatcatggt atatagttct acgtcgtatc tattattaga
    58141 aaacggacaa aatccgtccg catcagtcat taaccagagt attttttggg tgcttagctt
    58201 gattgctatc gcactacttt ataaaatgaa aacagatgtt ttgaaaaacc agcgtttgat
    58261 catggcagcc atcgcagttt tgacgatttt gctgctgatt gttgtgtttt ttggtaagga
    58321 gatcaatggg gcgaaaggtt ggctgcaaat tgcaggattc tcgatccaac ctgccgaata
    58381 cctaaaaatc atttctatct ggtacctgtc tctgacttta tctaaaagac aaaacagtgt
    58441 acaaaaagat tttcttggga ctgtgaaacg tccacttgca atggttatcg gacttactgc
    58501 attagtcgct attcttcccg attttggtaa tgcggcagtt atctttttga ttattttggt
    58561 tctgctttta gccagcggag tcaattatgt gtatactttg attgtaggag taggaggctt
    58621 ttgtctaagt acttttacga tctggctgat caatatcaca aacggaaaga tcttacccgg
    58681 acgtttacag tatatctata atcgatttgc tatttaccaa aatcctttct cagacgaatt
    58741 aaataaaggg catcaactag tcaacggcta ctatgccatg ttcaacggag gactatttgg
    58801 caggggatta ggtaacagta tccagaaaaa aggattttta caagaagcac aaaccgattt
    58861 tatttatgca atcgtagtgg aagaactggg agtgattatg gggattttga tattagcgtt
    58921 gctattcttt atgattgtgc gcattgtcct tgtaggtatc cgttcaaaag atccgttcaa
    58981 ttcgcttttg tgcattggga tcggcgcaat gtttttgatc caagtatttg tcaatcttgg
    59041 aggaattact ggcgttattc ctttgacagg gatcacgttc ccatttttaa gtcaaggagg
    59101 ctccagtttg ctgatgcttt cgatttgtgt cggctttgtc ctcaacatca gtgcggatga
    59161 aaaacgcaag agcttaggtt tataataaaa aaagatgtag gagtggttcg atgaaaaaag
    59221 ttttagttgc taacaggggt gaaatcgctg tacgtgtatt tcgtgcttgt acagaattag
    59281 gaattaaaac tgttggaatc tatgcagaag aagatgaata ttccgtacac cgtttcaaag
    59341 cggatgaagc atatttagta ggacaaggaa aaaaaccgat tgatgcgtat ctagatattg
    59401 aagggatcat ttctatcgcg aaagaatgcg gagctgacgc tattcatccg ggttacggtc
    59461 ttttgtcgga aaacttgaat tttgcgcaac ggtgcgaaga agaaggcatc atttttgttg
    59521 gacccaaact gcatcattta gatatttttg gagataaaat caaagcaaaa gctgcagcta
    59581 tcgaagcggg gattgcttcg attccaggca cagatggacc aatcgcttct atagacgatg
    59641 ccttggagtt tgcgaaacaa tacggttatc cgattatgat caaggcagct ctaggcggcg
    59701 gcggacgcgg catgcgtgta gctcacgatg aaaagagtgc aagagagggc tacgaacgtg
    59761 cgaaaagcga agcaaaagca gcctttggta gtgatgaagt gtatgttgaa aaatacatcg
    59821 ctaatcctaa gcatatcgaa gtacagattc tcggggatac gcatggaaac gtcatccatc
    59881 tttttgaaag agattgttct gttcagcgcc gtcaccaaaa agtagtagaa gtagcgcctt
    59941 gtgtctctat gaatgaacag caacgtcaaa aaatctgcca ggcagctgtc caattgatga
    60001 aatatgtcgg gtatgtcaac gcaggtacag tagaatttct tgttgaagga gatgattttt
    60061 actttatcga agtcaaccct cgcgtccaag tggaacatac gatcacagaa atgattacag
    60121 atattgatat cgtaacaacg caactgttga ttgctcaagg tttggatctg cataaagaaa
    60181 tcggattgcc tcagcaagaa ggaattaaat taaatggttc agccattcaa tgccgaatca
    60241 ctacagaaga cccgttgaat aactttttac cagacacagg gaaaatcgat acgtatcgtt
    60301 cacctggtgg ttttggcgta cgtctggatg tcggaaatgc ttacgcgggc tatgtagtga
    60361 caccgtattt tgattcattg ctagtaaaag tatgtacgca tggtgctacc tttgaaacag
    60421 ccattcagaa aatggaacgt tgcttgaaag aatttagaat ccgtggagtg aaaacaaaca
    60481 ttccatttat gctcaatgtc atcacacatc ctgaattcca atcaggaaat gcaaaaacaa
    60541 ccttcattga tagtacagca acattatttg aatttcctcg actacgagac cgcggaaaca
    60601 aaacgatgaa atatatcggt gaaattactg tgaatggttt tccaggtatc gaaagtggcg
    60661 aaaaaccatt ttatgaagaa ccacgtatgc ctaaagatct aatcacaaga actgattacg
    60721 taacggctaa gaatgtatta gatgcgaagg gtgccgatgc tttggtcgaa tggatcaaag
    60781 gacaggaaaa tttgttgttg acggatacga catttagaga cgcccatcaa agtctgctag
    60841 caactcgtgt tcggacaaag gatttcaagc agattgcacg tttgactgga gaaggtcttc
    60901 ctgaactctt ttcaagtgag atgtgggggg gagcaacatt tgatgtggct taccgctttt
    60961 tgaatgaaga tccttggcaa cggttgagaa aaattcgttc tttaatgcct aatacgttgc
    61021 tgcaaatgct tttcagaggt tcaaatgccg ttgggtattc taattatcct gacaatgtat
    61081 tggtggaatt tgtcaaagaa gcagcagcgc aaggaatcga tgtatttcga atctttgata
    61141 gtttgaactg gactccacaa atggaaaaaa gtatccaagc tgtacgggat actggtaaaa
    61201 tcgcagaggc agctatttgc tatactggcg atatcaacga tccaagtcgg gcaaaataca
    61261 atgttcagta ttataaagac atggcaaagg aactagaaca actgggtgcg catatcattg
    61321 caatcaaaga tatggctggg ctgcttaaac cgcaggcagc ttatcgtttg attagtgaat
    61381 tgaaagaaac gacagatctg ccaatccatc tgcatactca tgataccagc gggaatggaa
    61441 tcatcactta ttctgctgct tcaaaagcag gagtagacat cgttgacgtg gcaatgagtg
    61501 caatgagtgg gaatactagt cagcctagca tgagcagctt gtattatgca ctggtcaatg
    61561 gtccacggct gccagaaatc acgattgaaa atgcacagaa attaaaccat tattgggaag
    61621 atgtgcgcat gtattacaaa ccgtttgaaa acggcttgaa tgctcctgaa acagaagtat
    61681 atatgcatga aatgccagga ggacagtatt cgaatctaca gcaacaggca aaagctgtcg
    61741 gattaggtca tcgctgggat gaaatcaaac aaatgtatca tactgtcaac ttgatgtttg
    61801 gcgatatcgt taaagtaact ccatcatcta aagtagttgg agatatggca ctatttatgg
    61861 tccaaaatga tcttacagaa gaagatattt atgaaaaagg cgaaacacta agtttccctg
    61921 aatctgttgt gactttcttc caaggagaac tagggcaacc cgttggcgga tttcctaaaa
    61981 aattgcaaaa gattattctt aaaggcagac ctgcattgaa tgaacgtcca ggtcttttgg
    62041 ctgaatcagt cgacttcaac gaagtaaaaa aagaattggc agaaaaaatt ggctatgaac
    62101 caaaacaaga cgaagtatta agctatttga tgtatccgca agtctttttg gactatcaaa
    62161 aagcatataa tcaatttggc gatgttacat tactggacac accaacattt ttccaaggta
    62221 tacgtttggg tgaaacaatc aatgtacaaa tcgaacgcgg aaaaacgctt atcattcgtt
    62281 tagatgaaat cggagaaccc gatatcgaag ggaatcgcgt actgttcttc aatttgaatg
    62341 gacaaagacg tgaaatcact atcaaagata attcgatcat cagtacggtc caaacccgat
    62401 tgaaagctga accgacaaac cgagaacaaa ttggagcaac gatgtctggt tctgttttgg
    62461 atgtattagt gaaaaaaggc gataacgtta aaaaaggtga tacgttgatg attactgaag
    62521 cgatgaaaat ggaaacggcc atcgaagcac gttttgacgg agaagtcgca catgtatatg
    62581 tgtcttcagg agacactatt tcgtctggtg accttttgat tgaagtgact gagaaataat
    62641 aagcgaaagg tggggaaaca tgaaaagact gggtttgttt tggctgattc tgctattagt
    62701 tattgtggca ttctacatgg aaccagtctt gtttccgcca aaacttgaag tgaaggaaaa
    62761 ctctgatcaa caaatatcaa accaaatgac aacgtggaaa taccaggagc ttaaagcaac
    62821 tggtttttct acttatatcg gaaaaccgat cgatcagctg gaaaaggatt ttggccaagc
    62881 ctatgatcga ttaactagcg gatttggttt tgaaacaaga tattatgaaa ataaagctag
    62941 tcatctcaca tttgaagcga acatccaaga cagcagagtc acaactgtta aagtactgca
    63001 aacagggcct gctgatatcg cgccgtttta tttagggatg acgatgcaag atcttacgaa
    63061 aatcactact atctatacta atttcacttt tgagtaccat gatacagaag ttggaatcga
    63121 actaatggaa gaggatatga attatcgtcc gctaattgct tttgataacg acacttttgc
    63181 tattttattt tttgatcaaa caacaaacgg cctattttca gttgtgtatt tagataaaga
    63241 tagtcttttg aaactattgc catatcaagt gtttggtgaa ggccttccac attatcaagc
    63301 agataaatcg gctgattggg aatcaatcaa tcgcgttaaa gaacgaaaaa gtacgacttt
    63361 attaaatatg cttagaagag aagacgaact tcccatatac aatcaaacac ttgcctttca
    63421 aaaccacagt cagctgctac tacatgattt tttgacaagg cctgaggaaa tattgtcaga
    63481 ggaacgtatg gctgattggc aaaaatcgct aggcagtgct caaacgacag tgaaattctc
    63541 actgacgaat gatgaattgg agaagttaat cagtaaacaa aaattagaaa atactagtgg
    63601 gctatttatt catccggcga ttgacgctac tttttcgttt ttatattggt acagcgatcc
    63661 ttacaaccat gaccgtttca tggacccggg aacggattct gtaggcattg ctttttctaa
    63721 agaaaatatg ttagtcttat tgcaagaaga aaacggagaa agtagtgata gcagtgatcg
    63781 ttaacgaaga gctgtttcaa ctggaggatc aatgcctcca agtcgctgaa atgataaaaa
    63841 agagtaaaac gatggcttct tatcttgaac ataaacagtc aatggaagaa tctgcaaaag
    63901 tttgccagtt gaaagaagcg ttcttgtcta agaaagaagc atttgaaaat gtagcggcat
    63961 atggtgagta tgcacctgat tatcgtgaaa aaaaacgagc tgttcgaaaa gcaaaaagac
    64021 agcttgatat ggatgagcat gttgcagctt tccgatatag tgaaacacag ctgcaaagtc
    64081 tgttggatca aattgtccaa gtacttgccg aagaagtttc taatgaaatc aaagtagatg
    64141 caggtagccc gttttttgaa acgaaaaaac actttggatg cggaggtaat tgtcatgcaa
    64201 ttagacaata gtgaatttga aataacaaaa cgacgcgggc tgatcgtatg ggtctacagc
    64261 ttaaaacaat taaagaatct gaaaaagttc ggcttgttgc attatgtctc aagaagaatg
    64321 aaatatgtcg tgctttactt aaatgaagag acattggaag aaacagaaga acgaatccgt
    64381 aaattacatt ttgttcgaaa cgtagaacgt tcttaccgac cggatatcga aatgaatttt
    64441 gccgaaaaaa taggaaacaa aacagtatta aaagacgaag gtttcgaaat tgaagagcta
    64501 aatacagaaa tccgattagc tgaacatctt taaaaatctc ttatgagcag tttttaaata
    64561 aaaaataact tggataacga tcgatttttg atcattgtcc aagtattttt ttggtatact
    64621 agtaccagta ccatttctaa ggagtgagta gatgcggata atatcaggaa attatggcgg
    64681 gcgccgttta aaaagtttag caggggcgaa caccagacca actacagata aagtaaaaga
    64741 atccatattc aatatgattg gaccatattt tgatggagag acagtattgg atctgtttgc
    64801 aggaagcggt ggtttagcta tcgaagcagt atctagaggc tgcagtcatg cggtatgcgt
    64861 agacaaaaat taccaagcgc taaaaatcat caaagaaaac atcgaaatta cgaaagaacc
    64921 agagaaattt accacaataa aacttgatgc ggacaaagca atcgaacggc tagcagcgga
    64981 gaaacaacaa ttcgactact tgtttcttga tccgccatat gcaaaacaaa aaataatcga
    65041 tcaaatccat aaaatggaag aactgggact tttttctaaa accgcagtta tcgtatgcga
    65101 aacggataaa acagtcgaat tgccagaaac tgtggctgga ttccatcaaa taagaaaaca
    65161 gatttatggc ataacggccg taactatcta tagaaaagag ggaaatcaat gaaacgtgca
    65221 ttattcccag gaagttttga tccttttaca aaaggtcact tagatacggt ggaacgtgcg
    65281 gctaaattat tcgatgaggt agtaattggt gtttttatca atacttcaaa gaaaagtttg
    65341 tttcccccag aagaaagaat gacattgatc acaaaagctg tttctcatct tcctaatgta
    65401 aaagtcatgc atcaagaaaa tcaattgacg gtagaaacag caaaagagat tggtgcagat
    65461 gcgttgatca gaggcatacg aagtatcaaa gactttgaat atgaacgaga gattgctcag
    65521 atgaatcatc atctatatga tgaattggaa actgtcttct tattagcaaa accagaatat
    65581 agtcacgtga gttccagtat tttaaaagaa gtactgcact ttggcggaga tgtgagcagc
    65641 tacttaccgc cagtgatcaa cgaagcttta gctggaaagc gtgaatcaaa tgaaagctaa
    65701 acaatggttg aaaacagtac tgattatcat atttggaatg ttgatcattg gatttgtagt
    65761 agttcccttg ccgtattatg tagaatcacc gggagccacg atcgatctga aagaactaat
    65821 tacggtcaat gataaggaag ataaaggttc tgggtctttc tctcttacct ctgttggtat
    65881 caggcaagct acaggtttct tagctttaaa agctaaaatg accccttttg aagaattgat
    65941 cagtgaagaa gaattgactg gtggggcgac aaatgaagaa tataatcaaa tgcaacagta
    66001 ttatatggat tcttcccaaa atgcagcaat cgaacaagcg ttaaaacttg cagacaagcc
    66061 ttataaaatg gaattcaaag gcgtatatgt catgagtgta gagaaaacat cgaatttctt
    66121 tggcaagctt tcagttggag atacagtaac aaaagtagac gggaaaagct ttcaaagtac
    66181 agaagcattt atggattatg taaaaagcca aaaagttgga caaacaatcg aaatcagtta
    66241 tttgcataat ggtgaagaaa aaacagcttc tggtgatctg atcgagttac caacagataa
    66301 aaaggcagga ataggtatca cgcttactga ccataccgag atcgaatcag atacagatgt
    66361 tcgtattaat tctggctcta tcggtgggcc atcagcggga ttgatgttca ctttagaaat
    66421 ctatgaacaa gtcactggta aaaacttgcg acatggtaag caaattgcag gaactggaac
    66481 gatcaacagt gaaggagaag ttggccgaat cggaggaatc gataaaaaag tggcaagtgc
    66541 ggataaagct ggcgtagaga ttttctttgc accagatgat acgatttcag acgatgttaa
    66601 gaaagaatat cctgaaataa aaaccaacta cgaagaagca aaagctgccg ctaaaaaatt
    66661 gaattcctca atgaaaatcg taccagtaaa aacagtacaa gaagcgcttg actatctaga
    66721 gaaaatgaaa tgaaactaaa taattaaaga ggggttgtga cacgagttct gtcacaaccc
    66781 ctctttttcg tcaaaaatct tcacgataaa aataaaaaca aaagaaaaaa ttgacgtaat
    66841 tagatgttga agggacgatt ttgccagagc tcactgtttc ttcaatttta aatgaaagga
    66901 gtaatgaatg atttttactg gtcattcttt gaaaaaagaa caatgataga aaaaatcagc
    66961 aagaaaatac tagcttcacc caaattgatc atttggatgt ctatgatagt tcttatcttt
    67021 tgtatcgcaa gtggtttttt attatcaaat ttgctatttt atagagaaac agaagagact
    67081 gctgcaaaag aaatactttc ttcgtttagt tccagtggag aagatccatc agctgaagga
    67141 aacgatgtag aagaggatgc tcgtccaaag gtcatgtaca ccgatatcaa aggaagtgtg
    67201 aaggagcctg gtatctattc cttttcttca gaagaacggg tctatgatgt actaaagcgt
    67261 gcaggtggat tattagaaga agcggatagc gatcgaatca acttttctgc gaagatagaa
    67321 gatcagcaag tcctatatat ccctgctgta ggtgaagagc tgccagaaca tcttgatcag
    67381 ttggcatcct cggaaggaaa acagcctaca gcagattccg agcctcccaa aataaatatc
    67441 aatacagcca ctccatcaga acttcagcag attcctggga tcggatcagt caaagcgcaa
    67501 gaaatcattc gatttagaga agaaaacgga tccttccaga aagtggaaga cctgcaggag
    67561 atttctggta tcggtgaaaa gacagttgaa aaactaaaaa acttcgttac aattaaatag
    67621 aatgattcaa aatcgactgg cacgagcaat aaatggacgc taatggatac taagaataat
    67681 ccaattctaa gagcttggca ggacgaggag gtagtatttt tggataggaa atagcttgat
    67741 tgttcgcaaa gaaacattaa aaaaaccaat gctgaatgtc tatctccaaa ataaaatatc
    67801 tgggattcat attatgaata cggctgtcag cggcaataac agtcaagctc tacgagaacg
    67861 ctttgccaaa gatgtactta gctatacagc agataaagta tttattctca ttgggacgaa
    67921 tgatctagca gagcataaac aactttctaa agaaacgtac caaaaaatct gcagtggatg
    67981 atagttcagc tgaaaaaaga ctatagttct gatcatgctt tctttatcac gccaccagca
    68041 gtcgatgaga caaaacaaaa atatcggaat aacaagttga tcaaagaata tatctctgtt
    68101 ctcacagaag tagcaaaacg ccatgattgt gaagtatcga tttttatacg gaatttcttg
    68161 aacaaatgga ttcgaagaat ttttataatg ggctaatgga tgacggactt catttcggca
    68221 gtgaagggta ggatcttttg tcagacagga tgattcgtta tatttaaaga atattttgtt
    68281 taagcaatga gtacttttta atcaacttga aaaaacggag gaatataaaa catgttagaa
    68341 aaatcttcaa aagcatgcga tacgaataaa gaaaaagtta ctgataagtc acataatgaa
    68401 cgcattcctt gggaccagta ctttatggct caggcagtac tgttatcttt acgtagtacg
    68461 tgtacacgtt tagaagtagg agcgacactc gttcgagaaa aaaggatcat tgctggcggc
    68521 tataatggag cagtcagtgg agatgtccac tgcatagatg aagggtgtta cgtcgtggat
    68581 ggacactgct tgcgaacgat ccatgcagag atgaatgcac tcctacagtg tgcaaaactt
    68641 ggtatcccga cggaaggttc agaaatctat gtcacacatt ttccatgttt ggcctgtaca
    68701 aaggctttgt tgcaagctgg tgtgaaaggg atcaattatc tccatgacta tcgaaatgat
    68761 ccttatgccc aagcgttgat cgaacaatta ggtgtatctg tacaccaagt tgctttagac
    68821 tcacagcatt tttcaaaact tcaaaatgaa ttaacaggaa aacatgtcta gcgttttaga
    68881 aaggcatgtt cgaaatcggt tgatttttcc ggcaattacg tcgatgatta tctgtttgtt
    68941 gtattacact gacaattggg gttatcgttt gcttcttgcc ggaatttttt tatggttttc
    69001 gctaaaagaa ggacgtttta tcgcgtactt gtgtattttg tgtagcataa cggtatccat
    69061 ttcttatagg ctacaagagt attcattttt agaggaaaaa acaacatctg cgcaaattac
    69121 tgtatttaca gatacgattc gtgcaaatgg taattttttg acaatggaag gaaggttatc
    69181 agagaataaa caaaagctcc agctttctta tcaagtaaac acagaagcag aattgaaaac
    69241 atggcttaac ttccgaggaa agggaataga attgactgct gaaggcgagt tcatctctcc
    69301 agcaaaaaag cggaatcttt ataattttga ccaagaagct tacttcagga accatagaat
    69361 ttctggacgg ttccaaattc aaaagattga acagatcacc atgcaaaatc actggcgaaa
    69421 ctggcttcag agaaaaagag gatcttttat ctcctttgtc caatctgaat ttccgcaaaa
    69481 aacaagtgta tatattaatt ctttattatg gggctataaa gatgcaggat tcagagaacc
    69541 agaagaaatt tttagggaga gtggtttgct gcatctgttc agtctatcgg gtctacacat
    69601 ccaattttat ttagggtggc tgtattattt atttcgacgt atcggatggc cactacacgt
    69661 cagtattatt ccgttgagtc tactaactat ctgttatgtt tctattgcag gaagttctat
    69721 ttctgttttg cgtgctagtt tgctgttttt attgcgattg ctagtcaaat tgctgaataa
    69781 gcattattcc accatggact tatttgcagt tgtcttttgg cttcttttac tatttgatcc
    69841 tcgatttctt tttcatgcgg gtggacagct tagttttttc atgagttttt tgtttatcct
    69901 gatttctttt gatggagatt ggttgaaaaa aacggcctct cacgtcctgt ttactatgat
    69961 cacgatgccg ttgattgtat ggcattttta tgagtggcct attttaggta gtctttttac
    70021 attactgatt gcaccggtat tcaagtttct ttttcttcct ggtgtgttat ttctcacact
    70081 cttcaatcgc tggctgccgg aaactttact tttgctgata gaagagggat taatcttatt
    70141 tgagcgaata atcgattttt cttcagggtc gtctctcatt ataggacgtt tgccccttgt
    70201 gctatctggg atgtatattt tcggattact gatttgtatg gataggttga aagaacaacc
    70261 tgtgaaacac ttgttttatg ttggtctttt ttcttttatt tttttgactt tgccattttt
    70321 acgcttttct tcaacgatcg cttttatcga tgtcggtcaa ggagacagta tctttttgca
    70381 aagtcctttt cgaaaagaaa cgattttgat cgatactgga ggtcagttac actttgaaag
    70441 agaaaagtgg gcgcaaggta tgatacgtcc tcctgctgaa agaaatatcg ttccatatct
    70501 aaaaggtcag gggataagtg taatcgataa actcatcttg acacacgacg atacggatca
    70561 tgtgggagaa ttacccacgt tatctcagca ttttactatc aaaaaaattt atatcggttg
    70621 gggcgctgga cgaaatgagc cattacaaag gcatttatct gaagctcaaa aaaatggtac
    70681 tgagatcatt gaaatcaaac atggggacag gatcagcggt tattatgatc tttatgtatt
    70741 gactcctttt gaaaagggag agggaagaaa tgaagattcg attggtctat ggatggaata
    70801 taacaatagg cgctttttat ttttaggaga tttgaatcaa gcaatggaaa aacaattgct
    70861 tttgatttat cctaatctga aagccgatgt agtcaaatta ggtcatcacg gtagccgtac
    70921 gtcaagtaac acagattttc tttcccatat tgaagcaaaa catgggatta tatcctgcgg
    70981 cgtaaataat cgttatggtc acccgcacag cgaagtattg gagacactag aagaaaatca
    71041 gataaaaaca tggcgaacgg atcaacaagg aatgatcacc tatcgctggc atccagtttt
    71101 tcatcctcat gggctagtgg aaacaatgat agactagtaa ttctcatctg ctcatggtag
    71161 gataattttt gaaagtggtg agtaatttga gtatacaagc agagttacaa aaaatacgta
    71221 aagaaaaact tgcatcttgt tacttgattt taggaacaga aaagtttctc caagatcaag
    71281 ttcggacaga aattgtcaaa aaaatccaat tagagggcga agatgactta aattttttaa
    71341 gttttgacat ggaaaatgca agtttagatg aggtagtagc tgaagcagag acgttgccgt
    71401 ttttcggaga tcaacggtta gtattcgtag agaatcctta ttttttaaca ggtgaaaaag
    71461 taaacaatgg aatcgaacaa aatactgatt tattgacggc ttatttgaaa gaaccattag
    71521 aatcgactgt attagtattc tttgcgccat atgaaaagtt ggatgaacgg aaaaaagtaa
    71581 cgaaacaatt gaaaaaaaca gcaattatca tcaatgtcca acagttgaat gaaaaagaag
    71641 tacgtcaata tctgatgaat accttagaga attcctcaat caaaatggaa cgaagtgcaa
    71701 ttgatttatt cttgaggttg accgacttag atttatccaa aatgatgaga gagttacaga
    71761 aattggtcct ttatggacaa aatcaacagc aaatcacggt aagagaagta gaagaattag
    71821 ttcctaaaac gttagaacat aatatttttg acatgaccca atatatttta aaagggaaga
    71881 cggaacaagc tttgcgttta tacgaagatt tagttatgca aggggaagaa acaatcaaaa
    71941 ttaatgcgat tttgctttct caattacgtt tatttttaca gacaaaattt cttgtaaaga
    72001 ttggttatca gcaggcaaat attgctgaaa cattgaaaat ccatccatat cgtgtaaaac
    72061 ttgccatgca ggaagtaaaa aaattcgatg aacatttgct ggtacagttg tttgatcaat
    72121 tagttgagat ggattatcaa atcaaaacgg gacaaatcga aaaagaatta agttttcaac
    72181 tattcgtttt gcgcacaggg cagttgttaa gaaatcgata agaagaaaga cgagggcaaa
    72241 gcatgcggga aggtcgtttg tttttgtcct ttttttgaca aataaaaaaa tcggccgaag
    72301 ccgattcttt tatttagcga tttttttgct taaacgagat ttgtcgcggt tagctttgtt
    72361 tttgtggatt agaccttttg attcagccat atcaatagct ttaacagctt ctttatataa
    72421 cgcatctacg ttgtcagcac ctgaagctac agcatcttca aattttttga ttgcagtacg
    72481 catagcgctt gtttgagatg aatttttcac attagcgttt tcgcttgtac gaacacgttt
    72541 gattgcagat tcaatgtttg gcattagttt cacctccgat aacttaagtt cttacgcgtg
    72601 ttaccgcata attcaacatt cattattata cctaaaggca gcgtgctttg caatatgaac
    72661 atgacaagtt tttttcctta acagtctttt attaggattt tttttcagta aaaacggata
    72721 gtacaagctg aaaaaaatca aaaagacgct attgtgggag gcaatagcgt caaaccaaac
    72781 tagattttta tttatctatt taaacaaagt ttgaatggga atgtttggta ctaagttatt
    72841 atataacaaa gagaaccgtt tgcaaagttt tttttgaaaa gggatacgat tccctgttgt
    72901 tctttgtttt tctattaaga aactgcagtt attttataac cagtatcctt tgatcagtaa
    72961 aggctattct ggtgtaccga ttattttaat gtattcatcg atatttttta tcaacatttt
    73021 ttttatgacc aactggttcc gacggttcca tttgccactt acttccagca catatttatt
    73081 taggggcaaa cgtaagatat attttgccaa atagcgatct ttaagcaggc cattgatcac
    73141 acggtcctca agctgcaagt tgatcccagt cagcatcatt ggctgttctt gtaaaacgta
    73201 cgtatcaatt aaaagacctt caaaagtttc cacaagcgtc ttcctttctt ttcaaagtaa
    73261 cctgaaaaca ggcactaatt cttccatttt gatattcgtt gaagtggatg cgatcattct
    73321 ttcttttgtc cactgtaatc tagtataaca aaaaagagca gaagagccaa aaagaaaaaa
    73381 gagcatcgaa ttctatagga agtctcattg gcacggccta aattattaca aaacattgat
    73441 taaaaaaagc ccctgcatcg caggagcatc ccctatatcg ttttaatagg tcaatcaaaa
    73501 agcagtatca aacacatttt gtatcaaatc aggaaataat agataagcta agcccagaat
    73561 caaaagtacc gctagaagaa accagccgat tgttcctatg aaatacttta atttttcgaa
    73621 cattgtatca cctcgttgtc agtatatcac ggatcagtaa attgggggtg ttaagaatct
    73681 cctaaaatag acaaaatttg ttctattttc ctaaagcaaa tacttgtaag atacagtaag
    73741 tgataccaga agcaatgacc ggtccggcgg cgattcctct taagaagaca acacctaaaa
    73801 tcgtgccgaa aacaagtgca acagtgattt gtggatcaga agcaatcaat cctacaccct
    73861 tggaagataa gacagccact agcccaccgc aaaaaacagc tacccagcca ataggggatt
    73921 taaaggcttc taatagatca cgtagaccga tttctcctgt tgcgataggg attagaatag
    73981 ccgctgtgat gatcgtcacc ccaatattga ttcctttgcc ttgaagccaa gggaaaaagg
    74041 aacgggaggc tggtatgagc ttcataatca aaacaaaagc tgccgctatc atcagacttt
    74101 gatttttagc taaaattgct acaagtaaaa taattcctaa aaaaagccaa ctttccatga
    74161 aatatattcc tcactttctt cgaaacaatc ataacatgtt tttagaaagt tacaaaagta
    74221 ggaattcatc cttcaagacc tattcataag ctgttttttt cataaaataa acaaaatcat
    74281 aaaaaaacac tttgcttatt tgacagaaga cagccgcgct ttgtatagtg gacaagactt
    74341 acattttttt gaagaatcag aaagatcagg taaaggtctt agtaaacgca agccattcag
    74401 aataaccaat attgtgcttc cttcgtgtcc tacaactcca agaggtagat ttaaaaattg
    74461 caggaagtta gaagcaatca acaagagaat aacaagaatc gaaaaaataa tattttgttt
    74521 gatgatccgc cgcattttta aagatagccg atgactgtag acaaatttat ttaagtcgct
    74581 tttcattaag acgatgtctg cgatatccat agcggcatct gtcccttgcc ccatagcgac
    74641 acctaaagaa gcggctgcta aagcaggcgc atcattgact ccatcaccaa ccatcgcatt
    74701 tacttggtaa agttcattct cttttttgac tagttgtgtt ttttcttccg gagtacagct
    74761 tgcatagtaa tgttcaatcc ctacttgttc agcaatggcc tttgctgtgc cagaatggtc
    74821 gccggttatc atacttgtat ggatgttttg ggatttaaag taggaaatta cttgttgggt
    74881 atttgctttt ggaatatcta gcagacctaa aacagcaatt aactgctgat ctttagaaag
    74941 ataaatcacg gttttgcctt gattttccaa acgttcgatc atttccgcaa tctctttgct
    75001 gattatcatc gtctcaggat catatgcgtg cttgccaact ttccattgcg cacctaaata
    75061 ggtagtttgt aatccaaaac cagtaatttc ttctattggt aagtattgga tttcttcagg
    75121 tattgtgcaa tcgaaatatt gcaaaatcgc atgagcaagt ggatgcgtcg ttttttgttc
    75181 catagcaaca agaagttgtt gtgccaaatc cttttcatca aagaaaaagc tgtcagttac
    75241 aactggtttt cctttagtga gcgttcctgt tttatcaaag gcaatcgctt ttaattcaga
    75301 aagcttttct aaatggatac cgcctttgat taaaatccca ttttttgcag aatttgaaag
    75361 agcagctaga gaagctggtg tagcagatgc gacaagcgca caaggagaag ccacaactaa
    75421 taataccatt cctcgataaa agctttcatc ccaactccaa cctaggaaaa agtgggggag
    75481 aaggatcatt agaggaacag caagcaaaat caattttaca taattatttt cgattcgttc
    75541 gataaatgaa gctgtttgag ttggcgtatt ctgcgcttct tctactaatt ggataatctt
    75601 agcaaagaca gtttcggaac tcgtttttgt tactcgaatc gtgatgggat ttccttgatt
    75661 cagcgttcct gcatacatct catcgcctaa atatttttct acaggcacag attcaccgct
    75721 taatgcagac tcatcgatcg ttgcctgtgt gctttctaaa aagccatcta tcggaacagc
    75781 tgctccttta gcgacaaata ccaagttatc cacctttagt tgagaaacat ctacttcttg
    75841 catttgtcca ttttctttta ataattgggc tttttgtggt tgaagattca tcaaagcagt
    75901 aatttctttt tgacttttat tagttgtgta ttcctctaat gcaccgctta aacaaaagat
    75961 gaatgtcaac attgctcctt caaagtaatt accgatcaaa caggcaccaa tagcagctaa
    76021 agccatcaat aaatccacat tcagtgtccg atcattccac aattctttca agccttccga
    76081 tgtttgttta tatccaccga tgataatggc actgatataa aaaagtgggg tgatggtaag
    76141 agagaatcgg tcgaagataa aaccgaagat cataaataaa aggcagccga ttgttgctgc
    76201 aaaagctttt ttctgattag tcatcgttat tagacctcct tgtcaaaaca aggatagcat
    76261 aataaaaatg taattgataa taataacagc taaatgagaa cgataatggt tatcatttgg
    76321 tgattaa
//
LOCUS       DAZUKZ010000003        68351 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000003 DAZUKZ010000000
VERSION     DAZUKZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.003"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..909
                     /locus_tag="ACG8EF_000180"
     CDS             28..909
                     /locus_tag="ACG8EF_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4945541.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            922..2532
                     /locus_tag="ACG8EF_000181"
     CDS             922..2532
                     /locus_tag="ACG8EF_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4945542.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2609..3268)
                     /locus_tag="ACG8EF_000182"
     CDS             complement(2609..3268)
                     /locus_tag="ACG8EF_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4945543.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3551..4216)
                     /locus_tag="ACG8EF_000183"
     CDS             complement(3551..4216)
                     /locus_tag="ACG8EF_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFS4945544.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4363..4968)
                     /gene="dhaL"
                     /locus_tag="ACG8EF_000184"
     CDS             complement(4363..4968)
                     /gene="dhaL"
                     /locus_tag="ACG8EF_000184"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFS4945545.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4996..6006)
                     /gene="dhaK"
                     /locus_tag="ACG8EF_000185"
     CDS             complement(4996..6006)
                     /gene="dhaK"
                     /locus_tag="ACG8EF_000185"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFS4945546.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5981..6376)
                     /gene="dhaM"
                     /locus_tag="ACG8EF_000186"
     CDS             complement(5981..6376)
                     /gene="dhaM"
                     /locus_tag="ACG8EF_000186"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFS4945547.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6530..6850
                     /locus_tag="ACG8EF_000187"
     CDS             6530..6850
                     /locus_tag="ACG8EF_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFS4945548.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6962..7819)
                     /locus_tag="ACG8EF_000188"
     CDS             complement(6962..7819)
                     /locus_tag="ACG8EF_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS4945549.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(7927..9230)
                     /locus_tag="ACG8EF_000189"
                     /pseudo
     CDS             complement(7927..9230)
                     /locus_tag="ACG8EF_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(9227..9736)
                     /locus_tag="ACG8EF_000190"
     CDS             complement(9227..9736)
                     /locus_tag="ACG8EF_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945550.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9911..12046)
                     /locus_tag="ACG8EF_000191"
     CDS             complement(9911..12046)
                     /locus_tag="ACG8EF_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS4945551.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12129..13784)
                     /locus_tag="ACG8EF_000192"
     CDS             complement(12129..13784)
                     /locus_tag="ACG8EF_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4945552.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13786..14100)
                     /locus_tag="ACG8EF_000193"
     CDS             complement(13786..14100)
                     /locus_tag="ACG8EF_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4945553.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14270..14530
                     /locus_tag="ACG8EF_000194"
     CDS             14270..14530
                     /locus_tag="ACG8EF_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFS4945554.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14544..14858
                     /locus_tag="ACG8EF_000195"
     CDS             14544..14858
                     /locus_tag="ACG8EF_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFS4945555.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15588..16142)
                     /locus_tag="ACG8EF_000196"
     CDS             complement(15588..16142)
                     /locus_tag="ACG8EF_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS4945556.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16268..18196)
                     /locus_tag="ACG8EF_000197"
     CDS             complement(16268..18196)
                     /locus_tag="ACG8EF_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFS4945557.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(18502..19218)
                     /locus_tag="ACG8EF_000198"
     CDS             complement(18502..19218)
                     /locus_tag="ACG8EF_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS4945558.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19219..21042)
                     /gene="glpO"
                     /locus_tag="ACG8EF_000199"
     CDS             complement(19219..21042)
                     /gene="glpO"
                     /locus_tag="ACG8EF_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFS4945559.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21056..22552)
                     /gene="glpK"
                     /locus_tag="ACG8EF_000200"
     CDS             complement(21056..22552)
                     /gene="glpK"
                     /locus_tag="ACG8EF_000200"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFS4945560.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22688..24148)
                     /locus_tag="ACG8EF_000201"
     CDS             complement(22688..24148)
                     /locus_tag="ACG8EF_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4945561.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24138..25433)
                     /locus_tag="ACG8EF_000202"
     CDS             complement(24138..25433)
                     /locus_tag="ACG8EF_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4945562.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25765..26442)
                     /locus_tag="ACG8EF_000203"
     CDS             complement(25765..26442)
                     /locus_tag="ACG8EF_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFS4945563.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26472..27563)
                     /locus_tag="ACG8EF_000204"
     CDS             complement(26472..27563)
                     /locus_tag="ACG8EF_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFS4945564.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27664..28098)
                     /locus_tag="ACG8EF_000205"
     CDS             complement(27664..28098)
                     /locus_tag="ACG8EF_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS4945565.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28682..30139)
                     /locus_tag="ACG8EF_000206"
     CDS             complement(28682..30139)
                     /locus_tag="ACG8EF_000206"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4945566.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30136..32031)
                     /locus_tag="ACG8EF_000207"
     CDS             complement(30136..32031)
                     /locus_tag="ACG8EF_000207"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4945567.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32089..33864)
                     /locus_tag="ACG8EF_000208"
     CDS             complement(32089..33864)
                     /locus_tag="ACG8EF_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS4945568.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33914..35410)
                     /locus_tag="ACG8EF_000209"
     CDS             complement(33914..35410)
                     /locus_tag="ACG8EF_000209"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4945569.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35604..37226)
                     /locus_tag="ACG8EF_000210"
     CDS             complement(35604..37226)
                     /locus_tag="ACG8EF_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS4945570.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37675..38988)
                     /locus_tag="ACG8EF_000211"
     CDS             complement(37675..38988)
                     /locus_tag="ACG8EF_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS4945571.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39233..39457)
                     /locus_tag="ACG8EF_000212"
     CDS             complement(39233..39457)
                     /locus_tag="ACG8EF_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945572.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39477..39899)
                     /locus_tag="ACG8EF_000213"
     CDS             complement(39477..39899)
                     /locus_tag="ACG8EF_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4945573.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(40043..40753)
                     /locus_tag="ACG8EF_000214"
     CDS             complement(40043..40753)
                     /locus_tag="ACG8EF_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFS4945574.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40771..41268)
                     /locus_tag="ACG8EF_000215"
     CDS             complement(40771..41268)
                     /locus_tag="ACG8EF_000215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4945575.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41305..41757)
                     /locus_tag="ACG8EF_000216"
     CDS             complement(41305..41757)
                     /locus_tag="ACG8EF_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4945576.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41892..42497)
                     /locus_tag="ACG8EF_000217"
     CDS             complement(41892..42497)
                     /locus_tag="ACG8EF_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS4945577.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42947..43567)
                     /locus_tag="ACG8EF_000218"
     CDS             complement(42947..43567)
                     /locus_tag="ACG8EF_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4945578.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43569..44438)
                     /locus_tag="ACG8EF_000219"
     CDS             complement(43569..44438)
                     /locus_tag="ACG8EF_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS4945579.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44461..44868)
                     /locus_tag="ACG8EF_000220"
     CDS             complement(44461..44868)
                     /locus_tag="ACG8EF_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS4945580.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44887..46176)
                     /locus_tag="ACG8EF_000221"
     CDS             complement(44887..46176)
                     /locus_tag="ACG8EF_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4945581.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46203..46505)
                     /locus_tag="ACG8EF_000222"
     CDS             complement(46203..46505)
                     /locus_tag="ACG8EF_000222"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4945582.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46530..48623)
                     /locus_tag="ACG8EF_000223"
     CDS             complement(46530..48623)
                     /locus_tag="ACG8EF_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4945583.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(49112..49876)
                     /locus_tag="ACG8EF_000224"
     CDS             complement(49112..49876)
                     /locus_tag="ACG8EF_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFS4945584.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(49987..50856)
                     /gene="mdcB"
                     /locus_tag="ACG8EF_000225"
     CDS             complement(49987..50856)
                     /gene="mdcB"
                     /locus_tag="ACG8EF_000225"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFS4945585.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(50881..51957)
                     /locus_tag="ACG8EF_000226"
     CDS             complement(50881..51957)
                     /locus_tag="ACG8EF_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS4945586.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(51981..52574)
                     /locus_tag="ACG8EF_000227"
     CDS             complement(51981..52574)
                     /locus_tag="ACG8EF_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFS4945587.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52578..54254)
                     /gene="mdcD"
                     /locus_tag="ACG8EF_000228"
     CDS             complement(52578..54254)
                     /gene="mdcD"
                     /locus_tag="ACG8EF_000228"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFS4945588.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54244..54543)
                     /locus_tag="ACG8EF_000229"
     CDS             complement(54244..54543)
                     /locus_tag="ACG8EF_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFS4945589.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54645..56291)
                     /gene="mdcA"
                     /locus_tag="ACG8EF_000230"
     CDS             complement(54645..56291)
                     /gene="mdcA"
                     /locus_tag="ACG8EF_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFS4945590.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56298..57209)
                     /locus_tag="ACG8EF_000231"
     CDS             complement(56298..57209)
                     /locus_tag="ACG8EF_000231"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS4945591.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57329..58186
                     /locus_tag="ACG8EF_000232"
     CDS             57329..58186
                     /locus_tag="ACG8EF_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4945592.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58373..58735)
                     /locus_tag="ACG8EF_000233"
     CDS             complement(58373..58735)
                     /locus_tag="ACG8EF_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFS4945593.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(58924..59106)
                     /locus_tag="ACG8EF_000234"
     CDS             complement(58924..59106)
                     /locus_tag="ACG8EF_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945594.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            59081..60520
                     /locus_tag="ACG8EF_000235"
     CDS             59081..60520
                     /locus_tag="ACG8EF_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4945595.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(60527..63079)
                     /locus_tag="ACG8EF_000236"
     CDS             complement(60527..63079)
                     /locus_tag="ACG8EF_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFS4945596.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(63193..64524)
                     /locus_tag="ACG8EF_000237"
     CDS             complement(63193..64524)
                     /locus_tag="ACG8EF_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4945597.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(64490..65968)
                     /locus_tag="ACG8EF_000238"
     CDS             complement(64490..65968)
                     /locus_tag="ACG8EF_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4945598.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(66273..68108)
                     /gene="tdc"
                     /locus_tag="ACG8EF_000239"
     CDS             complement(66273..68108)
                     /gene="tdc"
                     /locus_tag="ACG8EF_000239"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS4945599.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        19440 a        14582 c        11700 g        22629 t
ORIGIN      
        1 atttttagaa aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa
       61 aagagctttg gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa
      121 gtcgttggat ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta
      181 gggattataa aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat
      241 agtttatcta tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat
      301 ttgactggag gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa
      361 aaaaaagatg aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat
      421 tcaaaaggaa atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc
      481 tatttatttg atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa
      541 gaagttgaaa aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt
      601 gaagtcgagc gactagcgga taaagtcgtg atcatacgcg aaggaacagt cgtagaagaa
      661 ggaacactcg atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca
      721 gagaatagtc acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc
      781 gctcaatttg ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta
      841 ggtgtgaaaa aatttgaatc tattccaccg actcttgagg atttattcat gagacattat
      901 gaaggatgag gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct
      961 caaaacctta gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt
     1021 tctggtggtt ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta
     1081 tacgaaacct tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat
     1141 gctgctgagt atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg
     1201 tttgctgctg tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt
     1261 ggtctgattg aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc
     1321 atcgctgaaa gtattctgat caatttgctt ttagcaatat cgatcagctg tacgatgatt
     1381 tttttccaag cagattcgat cactgcagaa ggtgcttggc tttttgggct atctgtaggt
     1441 agtatgggga ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca
     1501 gccagttcga atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata
     1561 acagatgtag ctgattcctc cctttcgatg tggaatcctt taggctggtc ttatttgacc
     1621 tatccattta caactaacaa ctggattcct ttgctttatt tgtttttgtt cagtctaatc
     1681 ttgataatca gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc
     1741 gaacgtgaag gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc
     1801 tatctaaata aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca
     1861 tacggctcta tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag
     1921 atgtttatcc aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtcgtc
     1981 ttgatttgtc tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa
     2041 acccacggac gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg
     2101 accaatatgt tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca
     2161 ctagggcttt ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt
     2221 cttaaagccg ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt
     2281 ctattcggat ggcttcctcg aatgggtaaa cttgcttata tctacttagc ttactccttt
     2341 gcactcaatt actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc
     2401 cctagctggg ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta
     2461 tcatgtatca gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta
     2521 ctagaaggat aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa
     2581 atccaacatt tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga
     2641 tccagacttc tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc
     2701 tagataaagt ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca
     2761 ggaaaaagtg atccttccct gtcgctttag ctaagtcaag cagatagaca gcgactcgtt
     2821 cttctacttt ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa
     2881 cgttgattgc taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat
     2941 ctttttgtct caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat
     3001 ttggaacgcc tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa
     3061 gctgttcctt tccgctagca gataattgat acactttcat actgccggag gccacgatca
     3121 ctaattgagg ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt
     3181 tgacaagtgc gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa
     3241 atgaaacaca taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa
     3301 taaagaaaca taacttccct tattccaact gataattttc ttttatcata gcataaacgt
     3361 caagcgaacc agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc
     3421 ttcttttgag accttaagta attgattcct atcaattttt taaatattta ctttttgaat
     3481 aatcaaaaac aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat
     3541 ttcgtgttgt ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt
     3601 gtaaggtttc agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt
     3661 tacccatttg aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat
     3721 cggacaaata gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata
     3781 tgtaataatc cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt
     3841 tatacgctgc aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga
     3901 tttggtattg tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt
     3961 cgcttcggta cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga
     4021 ttttaggttt gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct
     4081 ctttccattt ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg
     4141 gatggctctc tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg
     4201 attttccaag ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt
     4261 gcgatatgga caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc
     4321 aaacaaaaaa ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac
     4381 atctaaaagc gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat
     4441 tgcacgctct cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc
     4501 ttttgcctgg tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg
     4561 cagccaaaca tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc
     4621 cgctgttccc tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc
     4681 ttttgtcgct ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc
     4741 gactttcgat atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg
     4801 atcggcttga aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc
     4861 tccatcgcca atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc
     4921 tgcaaattgc gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt
     4981 ttatcttgag attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca
     5041 ttgtgtctcc gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat
     5101 aaaattaccg acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac
     5161 atcattcatg aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac
     5221 acctgcttct tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc
     5281 cagtacttta gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac
     5341 accatactcg atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt
     5401 tgctgctcga agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc
     5461 tttcaactca gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag
     5521 gacagtacct gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc
     5581 atcatctacg atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat
     5641 atcgaagttc aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact
     5701 atccgcttcc ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc
     5761 aagcaccgct gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc
     5821 gcttcctcca ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg
     5881 ggaagattct acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc
     5941 ttcgacaata ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc
     6001 cggcattttg tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg
     6061 ctgctgcaaa agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct
     6121 cttcttccaa catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa
     6181 aaacaaaata actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt
     6241 ctgatccgat ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt
     6301 gcatctgctc aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca
     6361 aaatactttt tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg
     6421 ctttcattaa tgaacaattc aagtatataa taaatttcta attttgggaa gatatgtgta
     6481 gtgcttcttg aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac
     6541 aacaacgaac agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga
     6601 ggtcattact gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg
     6661 cggccgctca aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga
     6721 aatggccgaa cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta
     6781 tgaagtatta ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc
     6841 cacgagataa gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc
     6901 actcaagaat tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt
     6961 tttatttctt tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta
     7021 aggaataact gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt
     7081 tggatggctc tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt
     7141 ctcctgccga atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt
     7201 ttgcttcaaa atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac
     7261 tggccgctgt gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct
     7321 gatttaattg ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga
     7381 cagcttccgc ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc
     7441 ccactttatt tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt
     7501 tgttttttgc agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca
     7561 aatgatcgat ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa
     7621 tcgtttctcc tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt
     7681 tttcagcacc agaagagctt atagttgttg aaggttctgt ttgagcaggt tcagaagttt
     7741 ccgcaaaagc tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg
     7801 gcttcattct ttttttcatc acttttccca tccttactac tttttaacta atttcttatt
     7861 ataagcaaaa atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg
     7921 catcttttat ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat
     7981 cgttttgact gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa
     8041 aagcatatat agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat
     8101 cagcgaagct agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact
     8161 gaatgtaaaa gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa
     8221 tccagcaaaa agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg
     8281 atcaatgaag atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa
     8341 attcgtcagc cagacttctg ttccaccttt tgcccaacgt tttctttgtc gatatagcat
     8401 cttcaacgtc acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca
     8461 gtcatttaac tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa
     8521 tccgcccaca tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata
     8581 aagaccaccg aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc
     8641 aaccgtttga gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac
     8701 cgcagcaata tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc
     8761 tggctccggt accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag
     8821 tccgatatta aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt
     8881 agcgtatttt ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc
     8941 tgtcactaat acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtatc
     9001 ttcgatcaca atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact
     9061 caagggaaca tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac
     9121 accaataaac catacaaatc cgcctaaaat cggataagaa acgagaaaaa atacataata
     9181 gacaaacaag tatctaagaa taaattacct gtatgggtga ttgagatcat ttcagttcca
     9241 ccccattttc tttatacaaa tctgcaatga atgtcgtatc tagatttttc tcttcggaga
     9301 cagaataaaa acggaccgtt tctctctctt cttttgttcc aaaacgttcc gtatacactt
     9361 cttcaagtaa ttcttgtctt tttttcaatt tttcatcttc atattcaaaa gatttattta
     9421 cttttttgta tagatttcga ttgttccacc tagtcaatac aaatgaggta atcaggaaac
     9481 tgaaaaaaat caatacaaaa aagccgatta agaaatacag cattttcttt tctgataaaa
     9541 aagcgtacaa ggaaaaatcc ttggctcttt ctggtctcgt taatgggaaa aagacccata
     9601 taaaaggaag aacaacgatc atccatccta aaagagccac taacgtttgc caaatcttta
     9661 accagtaatt accttttgaa aaatacttat ctttcaccaa aattgatgca acttcttctc
     9721 ttgttacttt tttcatatcc tcacctcacg tttttttgtt acgacgtagg agtttaccat
     9781 acatttcatt tcttttcaca taaagtgttc ctaaattttt cctaagaaaa agaacaaaac
     9841 atttctcatc agcaaatgaa aaatattttg ttctttttat cagttgttct ttttattttt
     9901 tatctcttaa ttatgttgct ctttccaata cgacaatgct tttcattcct ctagatatcg
     9961 ccgtgtacat ttgtctgcgt cttctttgct catctccatc gttttcaaat ggatataaga
    10021 ttacatgatc aaattccagt ccttttgcta gactcaaagg aatgatttgg cagttttcta
    10081 atccatatga atttttttta atcgattctt gtagcgtttg ggtttcctct tctgtttggg
    10141 tgataactgc cgctttttct ccttttagag aaaccaaaat ctcagctagt attttcaaca
    10201 attcttcaga ggatcgaaaa gagcggtatt cgggttcttt tccttgcggt cggatcgata
    10261 cgatgtcgat cttctcttga tccacagcat aggttttgaa aagctctgtg attgctccgc
    10321 tagaacggta actatttcta agatcatacc gatggatcgg cagatggtag tcgtcgaaac
    10381 aacgcataat atcagcaaaa gtagtggaag aattaaagat tgcctgattc tcgtcaccta
    10441 ctaaagtaaa gtgagttttt ggaaataatt cgatcaatag acctaattgt gccagggtat
    10501 aatcttgtac ttcatcaatc aatagatagc gaaatgctgg tacatttacc ttttcaacaa
    10561 gtaaatgacg aatagctaat ataatgactg cttcatcaac agtacgtgtg ggttgccaag
    10621 cataagctga gccatatcgt ttttcataaa ttctttcaaa caactgatgc tcatccaccc
    10681 atgccatttc ttcgatttgt ctggttattt ttcgatattt tttttgtaaa agtttccttg
    10741 cataagctac aatactttgt tccgaatcat cagaaatcaa tttaccgaat aattccaact
    10801 gcctatcttc tgtcaacgag agtacctgat tctgcacagc aggacttttt gcttgtttca
    10861 aaagatgatt cttccattcg ctcatcagtg cctttttcgt tgcttgaagc ctatcgatca
    10921 atttaggatg agcaggaact ttctcatata ttttttcgat cgttttttta gaaaggactg
    10981 tttttccatt tttagtaagt ccacggataa aattcggatg atctaaaaac aaaggatctg
    11041 attgttttag attatcgata aactttttgc tacgcaaacg ttctgtctgc tccgatacat
    11101 tttctcccgt gatacgtttg aaataagcct cttcaccttc tatttcctct gctgaaagct
    11161 gtgaaaccag ctgtatgatg gttagattca gtggatttct ttcacctaat gatggtagga
    11221 cattcgaaat atagtcgata aaccgatgat taggtgatag aatcagcaag tcatcggatg
    11281 tcatcgtatc tcgatataaa tagagcagat acgcaatacg ctgcatgata gcagaggttt
    11341 taccacttcc tgctacgcca tttaccaaga tattttgact tttcacatcc cgaatgattg
    11401 tgttttgttc cgattggatc gtagcagtga tagccttcat ttcattggtc tcatcttggc
    11461 ctaagacttc taataatacg tcatcttgaa tagctacaga cgtatcaaaa aacttgacca
    11521 aacgatctct ttccaaaaga aattgtcgtc tttctttaat aaccgtatcg attttatttt
    11581 gacgaaccgt ataagaagat ggaccaatca tgttattgta aaaaagttcg gcaattggtg
    11641 aacgccaatc ataaacaaga ttttcatctg catcatctgt gaaatttgct gtaccgatat
    11701 aaaaggattc ttttggctca ttatccagaa agtctacaac gacttttcca aagtagggac
    11761 ttttcaataa acgttcgatc tttgctagct gattttctgc gtttttctgt ttgatattca
    11821 tctgatcgat ttctctattt ttcatttcaa tcatcgaaaa agtatccagg ttatctaagt
    11881 aactgtcaaa attcaatcga agctcgccag ccatttcttt caacgactgg tttccttcta
    11941 tttttgccgt atccatcgat tcccgcagtt cgtttttttt atcaattagc ttttgataca
    12001 cgttcttcag atatgcttct tctaactgtt tttgtgtgtt gaccatacac attctcctct
    12061 ttttttaaag tggtggaacc gattagttta cctcttttaa gagtcgaatg caagcttttt
    12121 aacgagtatc atttgttagg atatatcagt tcttcaggtc ggacagtttg atataaagaa
    12181 aatgcaccat caaggttttt tacgtggtag ccagactgct tcaagattct ttccgctaga
    12241 tagctacgta atccgctatg acagctgacg atatattctt gttggctgtc taattcattt
    12301 aatcggtccc gaagttcatt caaagggata tgtttggctt ccttgaattt gcccttttcc
    12361 aattcttctg ctgttcgcac atctaataaa atttttcctt ttgctagttc tgtcggcaat
    12421 tcatgccatt gtacggtttc actaagccct tctgcaatgt tcattgctgc atatcctagc
    12481 atgtttactg gatctttggc tgagccaaat ggcggtgcat acgtcaattc taattccggt
    12541 aaatcgaaga tcgtcaaatg ccctttgatt gctgttgcta aaatatcgat ccgtttatcc
    12601 acaccttttg ccccaacacc ttgtgcacca taaatctcgc cagtctctgg atggaagatc
    12661 aacttcagta aaatatctgt tgcttctgga taatagcttg catgatcttt tcctgataca
    12721 tgaatgactt caaaagacag ttcgttttct ttggctgttc tttcacttaa accggtggat
    12781 gcggcagtca ttccaaacgt tcgtataatt gctgttccaa taccgccttg atttcttcga
    12841 gaaattccgg aaatcgtgtc cgcaacttgt ctaccttgac gattggctgg tgaagcaaga
    12901 gaaataagtg catcttttcc agtgatttgc tgttttacaa cgatagcatc ccccaccgca
    12961 taaatatcag gaatattcgt ttgataacgt tcgtctacta ggataccccc gcgtatgcct
    13021 aattcgattc cagcggtcac tgctagctgg ttatcaggac gaacaccaat ggataaaata
    13081 actaaatcag cagggatggt acgcccatca tccagtcgca atcgtttccc gtggtcttca
    13141 aatccagcga cagcatgaga agtaatcact tctactcctt gatgagacaa ttctttttct
    13201 ataaaagctg ccatttcttc atccagtgtt ggaagaatat gtggtgcttg ttcgatcacc
    13261 atgacttcta aacctcttct ttttaggttt tctgccattt ccaatccgat gaatcctgca
    13321 ccaacgatca cggcacgctt tggctgtttt tctaatgcat gcatcacttt atcgatatct
    13381 ggtacatttc taatagaaaa aacattgtcg gcttctgtta tccctggaat cgatggaaca
    13441 actggttttg caccaggaga taaaaccaat gcatcgtact gttcaatgtg tttttgaccc
    13501 tcatgtacga cttctaccgt tttgttttcg ggaaagatgg ctgtcacttc atgatttgga
    13561 cgcacatcta aacaaaaacg agcggataat gattctggcg tttggacaag aagattttcc
    13621 cgttcactga tttctcccga aagataataa ggcaagccgc aatttgcaaa agaaacatac
    13681 ggtccttttt caaaaacaac gatttctgca tcttccatta atcggcgaag ccgtgtagca
    13741 gcagacattc cacctgctac acctccgaca ataacgattt tcatcttatt tccctcctac
    13801 tgttcgatca aaccattgat tcatgccacc acgtacattc actacatcat aaccagattt
    13861 ctttaattct ttagctgctt gtttactcct catccctgat tgacaaatca catagacggt
    13921 cttttctttg tctccatcat atctgtttat actttgtaat ggaacatttt tagctccttt
    13981 tatatgcccg ccccggtatt ctgcaggtgt tctaacatca agtaacgctg gattttccaa
    14041 aagcttttcc tttaattctg ggactgttat cgattttatt gattgaaata gcaaattcat
    14101 tttctgcacc tcatttacat ttttataaat acctcatggg gtatattaaa caggagcttt
    14161 attaatatgt caaccagaat gactcttcat ttttttcact actttttata tttgcataaa
    14221 ataccgataa ggggtattat ctaattataa aaatagagga gaatgtgtta tggagattga
    14281 taataaaaat atcctgaacc gtttgaaacg tacagaggga caaattcgtg gtatccaaaa
    14341 aatgatcgaa gacgaaaaag aatgtatgga tgtcatcact caattaagcg ctgttcgttc
    14401 tagtattgat cgcgtaatgg gcatgatcgt tgcagataat ctgaaaaatt gctttgagaa
    14461 acctgatagt aatccagaag aacaagcggc taaactagaa caagcgatca agatgattat
    14521 taaaaagtaa aggaatgatt tcaatgaaag tcgttttcca tattgatgaa ttagaaaaat
    14581 ggtcagaaac agggaaaaac gtaaaaaacc tgataaaagc ttctccagaa acgacgattg
    14641 tcgtatctgt aaatggcatc gcgattaccg gttatctaga ctctgccaat gcagaattct
    14701 tagatttgca aggagtcgtt tttcacgctt gcgccaatgc tatgcgtgct aatcatattt
    14761 cagaaagttc tttacctgaa caagtaatcg ttgtaccagc aggtgtcctt gatttagttg
    14821 aactgcaatc ccaaggttat gcttatatca agccttagtc agtagaggtt gcgacagaag
    14881 tgatcagctt caagaaattt cggcttattt ttcgaagaag ctacttctgg aaaaccgtga
    14941 atcaaaggtt gtgaaaacgc tgtcctgcat caagtaataa gaacagccaa acaaaaatag
    15001 cttctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    15061 ttaaggaaga ggttgtgaaa tcagcggttt gacctaagta ttagagaaaa aaacgggaaa
    15121 atagctcctc atattttgcc gttttttgag ctaataccga aggtcagctg attgaacacc
    15181 gtttaaggaa gagattgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
    15241 aaatggattt ctacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaat
    15301 accgtttaag gaagaggttg taaaatcagc ggtttgacct gagtattaga gaaacaaacg
    15361 gaaaaatagc tgctcatatt ttgccgtttt ttgagctaat accgaaggtc agctgattga
    15421 acaccgttta ccaagaggtt gtgagcctac catttaactc cgaatcacaa gaaataattt
    15481 caaaaacaac ttctcctgtt tttggtttgg ttggcttatt atcgagaagt agctactggg
    15541 agtctattta tcaaaaaaag gaatgcgaca taggtcacac ccctttttta ttttctttga
    15601 aacatctttc ggtactcgac agcatacaag acaaaagatt gtaaaagacc gtaagtgatg
    15661 acggcaaaaa tgactctcac ccacagcgtt ggtaattctg ggaaaagcag caaaaggatc
    15721 atgccaacat agatgacagc agtcgctact ttgccatacc acttcgctcc acttaatttg
    15781 atatttttag atagcccgat taatccagct acaaacataa agccttcttt tatcaagaat
    15841 agaccaaaca acagccaaag ccacggtcga taccatgcca tagacaaaat cagaaaaagt
    15901 tgtgtcaatt tatctgcaaa cggatcaagc accttcccta agtctgttac ctgattatat
    15961 ctccgagcaa tgaccccgtc tagataatca gacaaacctg agacaacaat gaccgctcct
    16021 gctatataaa atgactgtct cagcacaaaa taaagataaa tcggcagcaa aaaaatacgg
    16081 aatatcgata aaagattcgg aatcgtttgt atttctttac gccagtctgt gcttaatttc
    16141 attttatcgc cctacctcct cagcactctt ttctttttat tttagcttac acggggaata
    16201 aaaccaatga tttcactgtt tttatttttc tttacagcca ctgatattat gagctaattt
    16261 gagataatca cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt ttgcgcattt
    16321 cttttactag tactttatga gctttttttg atcctcgctc taggtcacgc tgccgtttac
    16381 gtgttttata atcgataaac agactgtcta aatcgtaacc tgtcgggtac agatcttctg
    16441 ctgaacgcac cagacgaaca cgctgacgag gcacgtggat tatttttttg tctaaatata
    16501 catcaatcgt atcttcacct atatcttcat aaactaacgc ttctttttgg tattcattga
    16561 caaatatccg atcacctttt gcaaagagct ggcgttcttt tttattctcc ttcctatttg
    16621 taactgctgt cttaggaaga aattcttttt tctgtgtccg ataggctttt tccgccaagt
    16681 aacgctctgc ttgatggatc aatttcatcg acatatccat tttatgagca atccacaaag
    16741 cttggctttc tcctgtttct ccaacccgca actgatattt aggacgaagt gcttcccgat
    16801 cgaaagccat agcagccggc acaaaatctt catgatctcc ggcgaatttc ttgatttctc
    16861 cataatgggt cgtggcaatg atcaatgccc cttgctcata catagattcc atgattgcga
    16921 tcgctagtgc agccccttca tttggctctg ttccactgcc aatctcatct aaaagtacca
    16981 acgtattcct ttttatcttt ttcaaaatag cagcgatgtt ttgcatatga ccagaaaatg
    17041 tactcaacgc attttctaaa ttttggtggt caccgacatc gacgaaaatc tcatcaaaga
    17101 cagcaagctc tgtcccttct tttgccggaa cttgcatacc gaacatcgcc atcaaagtca
    17161 ataaaccgac tgtcttcaac acgactgttt tgcctccggc atttgctccg gtgataatca
    17221 agcctcgata gtctttaccg atttcaacat ccaatggtac cgcatgctca ggtaaaaatg
    17281 gatgtctgcc ttgcttgata atgatatgtt ccgatttatt gattctaggt gtgcttccat
    17341 tgatttctcg gctaaatttt gcccgagcaa agatgatatc taacaccgtg atcgtttcga
    17401 tcaataaatc gatcacttct tcgttttctg cgatagcacc agtcaatgcc gccaggactt
    17461 gatattcttc actgatttct tcagctttta acaactgata gtgttcattc aatttagaaa
    17521 tcacagttgg ttcaataaaa accgtcgttc ttttatttga ttcatcgata atcgttccat
    17581 ctattttatt tttatagctg gctttgatag gaatcgtata gtattcgcct ttttgaacaa
    17641 tcattgcttc ttggatcatt tctttgtttt tcggatgacg caagaatttc agtaattttg
    17701 actggatttc tttttcgatt agttgtaatt gtttgcgtac tttacgcaaa ttacgtgacg
    17761 cgtcgtcact gactttatta ttttttattt tctggtcaat caactcttct acatttatca
    17821 aatcaggaag atgtttgcta taagcaaaca acaagggtgt ctggtattga ttcttatcaa
    17881 aaaactttgt gatcatccga ctgcttctaa gaaaatccgc atattcgatc aaatcggttg
    17941 gctgcagaat caaccctttt ttgacttgtt ctgtcagtgc atcgatcctc ggcaacccca
    18001 taaatggaac atgctgactg ctttctaaga tcaagcgtgc ttctcttgtt tcttcttgac
    18061 gatccaagac agtttgtagg tttgtcgaga ccgtcatatc gctgattcgt tttttgctat
    18121 agtttccaat tgctcttgct tggatttcct ttttgatccg atcaaattgt aattgactaa
    18181 tcatttcgtt gtccatattc tctccttttc ttgaagagat acctgtccca aaataaataa
    18241 gggcctagag ataaataaaa ggatagaaca cagcttaaaa aagtcatgtc ctatccttat
    18301 tagaatacaa agatattgtt ttcctgacag gtaccgtttc ataagcacac caaataaact
    18361 ttgtttattg gctattcgac ttactgatta gataccagct acactcacaa aacatacccc
    18421 attcttctag gtcttattta ttttccactt aactatagca agacacattt tggttgtcaa
    18481 actttagtat gttttataga tttacaccat taatgaatag acacctaccg cgataattgc
    18541 tccgatcatt ggacctgcga ttggtaccca tgaatatgcc caatctgaag ttcctttatt
    18601 agctattggc aataccgcat gtgctaatcg aggaccaaag tcccgagcag ggttgattgc
    18661 atatcctgtt ggccctccta atgaaagacc gatggctaag atcaatccac ctacagcgaa
    18721 tacgttcgtt ccaggtgcaa attcgttttg tccaaaagct agcaacccaa gaactaatac
    18781 aaatgtacca attaattctg tgatcacatt tgctggataa ttccgtatag caggacctgt
    18841 tgcaaacgtt cctaaaatcg cgctttcatc tttcgttgca ttccaatgtg gcaaataaga
    18901 aagccaaaca agaatggccc ctgcaaaagc tcctaaaact tgcgcaatga tgaaaggaac
    18961 gacaagattc caactaattg cccctgtcat cgccatagct agagagactg caggattcaa
    19021 atgagctggg cccatgaatc cagaaatata gacagccatt gttacagcga gtccccaacc
    19081 aaatgcgatg acaatccagc cagaagcctg tgctttactt ttgtttaaat tcacagccgc
    19141 acacacacca tctccaagca aaactaggat cagtgtccca ataaactctc ccatgatttg
    19201 agtcatatca ctgttcattt atttctcccc ctttagttcc cgcagatctg attctgcaat
    19261 caatgcttct aaacgtttag tctgttgcgc cttctcctct tctgtccatt caaaatatcc
    19321 agcaattgca tcgatcacag gttgcttgat ctcatcaagc tgatctcgtt caaacaacag
    19381 atgatttgta cgacgaatga gataatcgcc tggggccaaa accatttctt cttctaaacc
    19441 ataacgtaac cgagctgact cagcgagact caaaccagga taaggtgcca tttcttttgc
    19501 taattcaaag atacgtcggg cattagtccc gtaaaaatca gcgatataag tagcatcttc
    19561 ttcctctaaa ccggctgcta ctccttcttt tgctaattct gtcactgttt cttctaattt
    19621 agttggatcg aagtttccac ctgaaatctg atatttttta gagtcgattt ctttcgtctc
    19681 tattccgtat tcttctttta ataattggcg tatcaatcgc aaagctcctt ctgccatctt
    19741 acggtaatcg gtgattttcc caccagataa agtcagcaaa ccatctggtt ctctttctaa
    19801 agaactacct ctggaaatcg tagaaggtgc tttttcatca cggctgtttt ccaaatggtt
    19861 caacacgtct tctacttcag cacgagaaac tttgttttcc ttatattcac ttactgtatc
    19921 gacaacttta ttgaagctct tgtctgaaat cgatccatta tctccaccat tgtaatcaga
    19981 accagaatta ccaatcaaca gtggacgaag tcctgcccag cttgcttcga tatcattcaa
    20041 tgtgatattt gcttctggat agcgatgatt gatcacatcc aatagataat ctacatcttc
    20101 ttgtgtgact ttcggatccg taaaatctcc ttggtaatcc gtatctgtcg taccaaagta
    20161 agtcttgttt tctcttggaa tagcaaaaac catccgccca tcttgctttc ctgtatcgaa
    20221 gtatgtgggt tgcggtacag gcagtttttt cgcatctaca actaaatgga tccctttagt
    20281 tggacgcatt ttaggagaga ctggacgcgt aaaattcaag tttcgtactt tatctaccca
    20341 aggaccactc gtattgatca ctaattttgc tttgatttcg atcacttcat ctgtcagcag
    20401 gtcacgagct ttgacgccaa cgatttgatc gccatcatat aaaaagccaa ccgctttcat
    20461 tttacttact agataagccc catcttcggc agcctttttg atattatcaa tcactaaacg
    20521 ggcatcattg ttgcggaaat ctagatagac acccgcgcct tttagccctt cttttttcaa
    20581 aaacggttct ctttccaata cttcttctgg tgtcagcgta taattctcat atttggttcc
    20641 tgtcacattt gccagtttgt cataaaggtc cattgctact ttgaccgaga acatattgaa
    20701 ggttgttgct ccttcgccct catagattgg caaaagcatt ggatctggtt ttgggatgtg
    20761 tggggcaata ctttgtacga ctgcacgttc accaactgtg tcagctacta cttccacatc
    20821 aaatgtcttc agataacgaa taccgccatg aacaagtttg gtcgagcgag aggaagtccc
    20881 ttctgcaaaa tcctgcattt cgatcaatcc tgttttgatt cctgatgctg ctgactggac
    20941 tgccacaccg gcaccagtga ttccgccacc gatgatcaac agatccaatt cttcttgctg
    21001 cattttttga atgctatctt gtcttgtctt gtttgaaaac atatggattt cctccttatt
    21061 caaatgcttg ggtagctttt actgcttttt tccaaccgtt gtaaagtttt tctcggcgct
    21121 cttcctccat ttgaacttca aatagcttgc cagcttcata aaattcgcgg atctcgtctg
    21181 tatctttcca ataaccgaca gctaatcctg ctaaaaatgc tgcccctagc gccgttgttt
    21241 ccaagttttc tgctcgttgg atcggtacat tcaagatatc tgtttggaat tgcatcagat
    21301 attcattgtt tgctgcaccg ccatctactt tcaatacagg aatcttgatt ccagtgtcat
    21361 cttgcatcgt atcgatgata tctctcactt gataagcaat cgactgtaaa gtagctttaa
    21421 tgatatcttc ttttgtcgta ccacgtgtca agccaaacat agcccctcgt gctgcctgat
    21481 cccaataagg agcacctagc ccaacgaatg caggaacaac ataaacctca tcttcgcttg
    21541 tagagcgttt cgctgcgtct tctgaatcgc tagctttttg caacatttga agtccatctc
    21601 tcagccattg aatcgaagaa ccagccacaa aaatacttcc ttctaaagca tagtaaacct
    21661 ttccatttat gccgtaacca attgtcgtca ataaattatt tttggataat tgcggttttt
    21721 caccggtatt catgacaata aatgaacctg taccataagt attcttgacc atgcctggtt
    21781 cgaatgccat ttgtccgaat aatgcggctt gctggtctcc tgccatccct gaaattggtg
    21841 cttcgcttcc ataaaaatga taaccttgcg tataaccata aacttctgag ttagaagtga
    21901 cttttggtaa catcactcga ggaatattta gtaaatctaa aatctcctga tcccaatcta
    21961 gctgatggat attgaacaac atcgtccgac tggcattcga gtaatcagta acatgtgctt
    22021 gtccatcggt cagtttccat actaaccaag aatcgatcgt tccaaacatc aattcgcctt
    22081 tttcagctcg ttcttgcgcg ccttctacat gatcaagaat ccagcgtatc ttcgttgctg
    22141 agaaataggc atcgatcact aaccctgttt tttcgtggaa aaattcttga tagccttcat
    22201 tttttaagtt atctgcaatt ccagctgact gtctggattg ccaaacaact gcatgataga
    22261 ttggacgtcc tgtatctttt tcccatatta ctgttgtttc acgctggttc gttatgccaa
    22321 tcccagcaat ctgatttggt ttgatccctg attcgatgaa cgctccggca ataactgact
    22381 gtacagaatt ccagatttca tttgcatcat gttccaccca actttcttgc gggaaatatt
    22441 gcgtgaactc tttttgagaa ctgccgatgt gtcttccttt cttgtcaaag ataattgctc
    22501 gtgagctagt tgtaccttgg tcaatcgcca tgatatacgt tgattctgtc attgtgattc
    22561 cctccattat atgcaaacgc tttctttaac tactcttatc atactactgt tttaaaaaaa
    22621 gaaactgtca aaattttatt ctttttttct ttttttgaca gttttttatt atgaagctat
    22681 tttttaatca attcttttct tttttcagcg tctctattct tcttttcaat cgaataatgt
    22741 cgtaaggtga cgcaaattct gacagataat attcacactg ttttttgttt ctctggcaaa
    22801 atgtgatcac caaatcatat tctttacctt cttcataact tccaatctca ataccgccga
    22861 ttcccctcag ttctctgatc agatattgtt gaaaagcaat acgataaatc ggcagacttt
    22921 ctaaatcgat tccaatcact actggttttg caatataaaa atcgatcatc aaaagaacat
    22981 tggcatactg gatcatcagg taactaagct cactatcttc agctcttttc ctcggcaatt
    23041 gctgcaccgc tgtttcctgt aatttcgcca gtaactgtgt taaggaatgc ccgagcattt
    23101 tctgctgctg ttccaaaagc cgatcacgct catagacttc gatctttcca acaaagaaaa
    23161 accattcatt gttgatctgg gaaatttggt agcctactgc cttttcttct ttgattttca
    23221 aacgacgagg acgataatgc cataacatcg tttcccgaat gtaagtatct aaaacagcgg
    23281 ttggtaactt tttgctccgt gtcaaatcat actgataaaa tatttcttcg tctactatcg
    23341 aaaaagaaac aaaaaagctg taaaacatca ttgtttcgta tacattcagt tctgctgctg
    23401 ttcgactaag atgcatcacg atgattggat cgattgcttg ataaagccgg tccgactgat
    23461 agagtaattt cttttctttc aaagttgcgt acgtactttt ttcattcaat agtctttttt
    23521 tcgtaatccc gagccagcaa gcaatctgtg cttctgctga tgccgtaaac gttgtttcta
    23581 atacacggtc caatcctaga atgaacggtc ttttttctgg tgtattttgc aaaaaaagcg
    23641 gtcgctgatc ttccgggata tattggaata gttcataata aaaatatcgt atctgtagct
    23701 cttctccgta taattgaccg tttttgattt gtaatccaaa ttcctctaag agctggttca
    23761 attctttgat ctttcgaaaa acagaagact cactgatatt aaacgctgtc gctaatcgaa
    23821 cgatggatac ttgctgatgt tctaataaat agacgagcat cttaaacttc aagctttcct
    23881 gcaataaaaa caatagaatt ttttccaaac ttacatcttc gtccaacaca agcagcgcct
    23941 gatactcatt tcgctgtact tctacttttt tttccatcat tcggccaatc aattcaatat
    24001 cttccaaata ttgatcaaac gaggcttttg acagttggac atgctctctc aaaccattga
    24061 tagaggctgt tcccccttgc agaacaaggt ttcgcaaaat caggatctgt aactgctctt
    24121 ttctttctaa taatgcttca atcttcatag gcagtattcg ccacctttga ccacaccata
    24181 tctggcacac ggtattctgg ctggaaaaat agctcatgtt cttctaactg ttcaaacgtc
    24241 catccttctt ctactgcaat tgtcagttgg tcaagaaggt ggaacatctc ttttttgctt
    24301 tggagctgtg ctccaagaat ctttttttca gaatcaaata gaaatttgag tttaactttg
    24361 ctttgattta ttgctgtaag cttgtagtct acgtatcctg tttcaatttt ttggggataa
    24421 aaaatgctct cttcttctgt caaacctacg cttcctaaga aatagccaaa ccactgattg
    24481 ccgactggac gataggttcc ctgatccttt gtctgtgagc cactaattgt cttgctgaca
    24541 tttcgtgctg tacggacagc gttatttact agagaaacat acattttttc atttgttgga
    24601 cgaaaagtta cttggatcgc atcgccaatc gcataaacgt ctttttgaga cgtttgcaag
    24661 tagtcatcta cccagatcgt tccatcatca ttcaatgtta attgttcttc ccacatctga
    24721 ttgtcggggc gagaatgatt agccagcaaa acaagatcat agttctcctt tcttttttcc
    24781 gtctctacaa tagcggtttc attttcggtc aaagacacaa cttgttcttc taaaaaaagt
    24841 gaaagccctt gatttttcat ttcctttact aacggttctg tcatttcagg atctagataa
    24901 cgaaaaagaa ttgactttcg gctttcgtaa agatggattt gtttttttct tgatgccaac
    24961 ccttcagcta tttcgattcc tgcttgtcct gcaccaataa ttgcaatttt ctttgcattc
    25021 atgatctttt gctgaagagc cgttacgttt tgtatctttt taactgggtg aataagcgtt
    25081 gattctgcag aaatatttcg aaacttttga tttgaaccag tcgctagaat caaacgatca
    25141 aaatccaatt tttttcctcc tgctaaaacg actttttttt ctttcaatcc tattactgtc
    25201 ttttctgtgt aaacatggat tcgatagcgg tcagtcagtt cttctttcgt gatccaagaa
    25261 agttgttttt cactggtaac ctttcctttt aatatcaaat tgaacccgct gggaatccat
    25321 cctaacttcg attgcttttc aatcaaatat atttcacttg ttggcatcaa ttggcgaaga
    25381 taaatagccg catgaatccc tgcaaacgac ccgccgacaa ttacaatgtt catctgcatt
    25441 cctctttctt ataaggtgtt ctactgctaa cttctcaatc agaaaacaca gtattcataa
    25501 ctcgctttca accttatgat acatagcgat gcagcagaat caaccctctc catcataccg
    25561 tgttctaggt ctgcttctca ataatgtact agtggaccta attgaagagt atcatttctg
    25621 aagagatgtt tcaaaaaatg tactcttata aaagaaaaaa actcgccttt tacgtacctc
    25681 tgtccaaata ggaaacagaa aagaacgaaa agacgagttt tagcgtgtgt aatgtaatag
    25741 ttagaataga cacatcattt atttttaatt ctttaatgct tcaacaactg cttcgcctaa
    25801 ttgatgagca gattgaggat tttgccctgt aagaagccgc ccatcaattt gaaccacttc
    25861 tgaaaaagct gctccagact gatagtttgc tcctttttct ttcaaagcat cttctgctaa
    25921 gaatggtact tcattcgttg taccatttgc ttcctcttct tcattactga acccagtcac
    25981 tgtacgtcca gctaaaatag attttccaga ctcatctgta actggcaaca atcctacaac
    26041 accgtgacaa acagctgaga ccagcccttc atttcgatag atttgtgaag cgactttccc
    26101 aagatctatt gcttctggaa aatcccacat tgttccgtgg ccaccagcat aataaaccgc
    26161 tgcataatca tttgcatcca catcatcagg acgtaaggta tttcccaagg cccgatttct
    26221 gaaatcttca tcttcataat aggcccaatc gatttcatcc atattcaaaa cactttgcgg
    26281 ttctaaaggt acatatccgc ctttaggact gacaaaatct gcatcgatcc cattttttac
    26341 taatacatca taaaaatgag tcagttcact taaccacaaa cctgtaggac gctcgatctt
    26401 ttcgtacttt tctacatttg ttaatacgat caatactttc attttcccat cctctttcta
    26461 taaaaattgc gttattgata acgtgctttc aagaaattat ataatgggct tggcaaataa
    26521 agctctttat atgcttcttc aaaaggtttc gttacaaatt catcctcacg gttttccaat
    26581 aatttctctg cccagtcagg atcgacgatc atttgctgtc caattgctgc aagttctgca
    26641 tatttcaata cctgctctac atcttcgcga ttgcggatcc caccgacacc aactaaaggg
    26701 atctttcctt gaatcgtgtt atggaccact tcaagaatcg atttatcgtt gtatttatca
    26761 gaacgagcta ttcgatcata catatttaat gaaacatgca gataatctag ttttgactca
    26821 cgtaatcgtt caagaagcca gatcgtgtct tcaaaacgga tacctggtgt ttcatattct
    26881 tctggtgaga aacgataccc tacgatgaat ggttttttag catattgttc aaccgctgag
    26941 aaaaccgttg ttaataattc ttcaataaaa cgataacgat tttccaagct accgccccat
    27001 tgatcctctc gtctattcga atgcggagag aaaaattgtt ggatcaaata cgtatttgct
    27061 ccatgcaact cgattccatc aaatcctgat tcaatcgctc tacgtgtggc tttgccaaat
    27121 gcttggattg tttcaacgat ttcttcttca ctcattgctc gtggtgtttc cgcatcagga
    27181 cgttttgctg caactgcact tgcagataca ggctgttctc cagataaagt tgctcgactt
    27241 gtcattcgtc caccgtggaa aatctgaata atcgcttttg cgccttctcc ttggattgct
    27301 ttcgctagct cacttaatcg aggaatcatt tcatcatgag cgatgctgag ttcacctggc
    27361 cagccttttc cgatatcttg gacatttgca gcaccagtaa taacagcacc aagaccttta
    27421 gaacgttgtt tataataagt gatctcatct tcggtgatca ctccattgaa aaagctgagt
    27481 tgtgtcgtca taggcgacag catcaatcgg ttcttcaatt gaactcctga aggtaaagta
    27541 attgattgat caaattgtga cattgtcatt cctcccataa ttcgctctga ttaacctatc
    27601 gagaatcatc tatcattaaa ccactcgatt agttgtacat gcaactaatt tgcttaaaaa
    27661 attttatttt ttttgtaact tatctgttgc atcattcatt gtctgggcta attctttact
    27721 tgcttcattt cccaaaactg ctttgtatga ctgatgaagt tcttcccagc taccatcgac
    27781 ttccttagca aattggcgtc cttgttctgt aagataaact aatgacaagc gaccttcgct
    27841 aactgttttg acgtatcctt tatattctaa tttttcaaca aaacgagcaa tcgttgatgg
    27901 ggcggaatag agcatttgtg caatatcttt ttgtggtatt ccatcttttt cttttaatat
    27961 caataaaata aaaccataag ttggtgccag acctgtcctt ctaaacgctt catcagctaa
    28021 tttattcaac atgcgatcta gctttttcac gctaaagaaa agacattcat ctaacgcttt
    28081 ttcaattggc ttttccattt catcccctcc attggcgtac atatcatagt atgattttta
    28141 ttttttgtca aaaaacacgg aataatgtcc gcctcctctg tctagatttg aaaaaaagaa
    28201 gtagccatct tcaaagaatt tcggcttgtt ttcgacaaaa ctccttctgg aacaccgtga
    28261 atcagaggtt gtgaaaaagt cgtttagttt tgagtatgaa gaaaaatttt gtgaaaatgg
    28321 ctttccacat ttttgcataa atttggctta ataccgaaaa actggctttt gaacaccgtt
    28381 tatatcagag gttgtgaaaa agccgtcctg catcaagtaa taagaacagc caaacaaaaa
    28441 tagttcctca tattttgtcg ttttttgagc taatgccgat ggtcagctga ttgtacaccg
    28501 tttattgaga ggtcgtgagc ctgccgttga actccgagtc acaaggagca atttcaaaaa
    28561 acagcttctc atgttttatg actatggtga cttgtgatcg aggagttggc aggagaatac
    28621 tgtttattcg taaagaaggg gctgtgacaa gacttttgtc acagtccctt atttcctttt
    28681 attataggat ttcgccattt gattggatga cttgtctgta ccaatcaaat gattttttct
    28741 ttaaccgttt ctttgtgcca ttcccttcgt catcttgatc aacatagata tatccataac
    28801 gttttgacat ttgtgaagta gaagcgctga cgatatcaat gcagccccac gacgtataac
    28861 caaataaatc tactccatct ttgatagctt ccttcatctg ctcaatatgc tgtttcaaat
    28921 agtcaatgcg gtaatcgtct tgaacggttc catcttcttc tactttgtct atagctccga
    28981 gtccgttttc agctatgaac agtggttttt gatacctatc atataattga ttcaaagcaa
    29041 ttctcaaacc cttaggatca atctgccagc cccattcact tgatggaaga tacggatttt
    29101 taattcctcc agttattaag tttccttgga ccccttccca attttctgga tgagcactga
    29161 ctgcactaga catgtagtag ctaaaagaaa caaaatcaac agggtaggct gctaagattt
    29221 cttcatcacc tacttctatt tttaagttga cacctttttc ttcaaacatt cgttgcgtat
    29281 acgctggata atatcctctt gcttgaacat cactaaagaa caaagaagtt ctttgctgct
    29341 caattgtttt ttgaatatca tccggatcac aagaatacgg aacaagaagc tgatagctga
    29401 tcatacaacc gacttgtgaa cctttgataa tttcatgaca agcttttgta gctaaagcac
    29461 ttgctataaa ctgatggtgt gcaccttggt atttcgcttg ttccaaatgg ggatggtttt
    29521 cttcaattac tcctacgcta acaaaagggt gatgttttac acagttgatc tcattgaaag
    29581 tcatccaata ttttacttta tttttatagc gtttgaatac tgtttcagcg aaacgaacgt
    29641 aaaactgaat aagctttcga ttaggccatc caccatattc tgtaaccaaa tataacggca
    29701 tttcataatg ggaaagagaa acgactggct caatcccata ttttagacat tcgtcaaaca
    29761 cttgatcata aaaaaataat cccgcttcat tgggtgtttc ttcatcacca tgaggaaaaa
    29821 ttcttgtcca tgcgatagac atccgatagc atttaaaacc catttcagca aataaacgga
    29881 tgtcttcttt aaagtgatga tagaaatcat ttccgtgtct tttaggataa taatacttgc
    29941 tgttgggatc ctgcgccgct tttacttgct gatgcgtcat catatgccat tgatctgtcc
    30001 atttttcttt aggtaaatcg ctgtcgaatg tatacacatc agacacagat aatccttttt
    30061 catctacatc ccatgcacct tcacattgat tggccgctgt cgctccgccc cataaaaatc
    30121 cttctggaaa ttttttcata tcgatccccc taaacttgct agttttcttg tttgattatt
    30181 tttcttatga ctagtaatga tatttttgaa tctactacta tttgtaacaa taaccattgt
    30241 ggtcggatca tatccctctt tgattaattc ttgcaaatct acttccatca ataaatcgcc
    30301 tttacgtact ttttttccat cagatactgc actttgaaag tatttgccat ttaaattcac
    30361 tgtatcaata ccgatatgga tcagtatctc tgctccttct tcactgacta ccccaattgc
    30421 atgtttcgtt ggaaaaactg ttgaaacttc cccgtttaca ggactgtaga tttttccgtc
    30481 cgttggaatg atcgcaaacc cttctcctaa agatccacta gcaaatgttg gatcgtttac
    30541 ttcagataaa tcaattattt cacctgctgc aggagtactt aacactatat cctttgagga
    30601 ttcttcttcg atttcatcct tgtagccgaa aagaaaagtt agcatacagg aactgatcat
    30661 cgctaagaaa taactagcaa ctagccaaac aaatccttca ccaatataaa tcgggaaaga
    30721 caaaatactt cttgtagcaa ctgccaaagc accggctcct gcggcaccag ctactccacc
    30781 aaatatagct ccacatatac aagcaatgta aaatggtcgt ttatatttaa gagtcacacc
    30841 ataaacagca ggttcagtga ttccaaataa tgctgtgata aatgcagata aagataaact
    30901 ttttactttc ttatttttcg ttttcaaaaa taccccaagt actgctcctg cttgcgcagc
    30961 tacggcagtc atacatgctg gacctagcgt atcacgaccg tagaaagaaa gattgttggt
    31021 cattactggt acaagtcccc aatgtaaccc aaaaatcact aacacttgcc aaaatccgcc
    31081 gataacagca ccagataaaa cagggctaaa gttataaaca gctaaatatc cagctgcaat
    31141 cagctgactt gcataatctg caattggacc tacaactaat aaagtaattg ggaccatcac
    31201 gatgatacaa atgaatggtg tcaataaatt tcgaatactt tcgtgcaagc ttttgcgaat
    31261 cttaggctca atataagaaa gaacccatac agctaaaatt gctggaataa ccgtacttgt
    31321 ataccttgct agaatcacgg gcatccctag aaatcgcaag gttatgctat cactatatgc
    31381 actgacaatc gtaggataaa cgagagctgc tgcaataacc atagcgatga aacgatctgt
    31441 attgaacttt tttgcagcag tatatgccaa aaatataggc aagaaataga aaaatgcatc
    31501 agctgctgca ttcaaaatga tataagttcc tgattcagta gttaataaat tcaaactcgt
    31561 acataaggcg agtactcctt tgatcaaacc acttcctgcc aagacgccta aagtcggggc
    31621 aaaaacagcg gcgatcatgt ctataaaaga gtttaaaatg ccttttttct cagttcctct
    31681 ctttccacta gatgcatcat cttctagttt catttgtttg gataatacgt catacacttc
    31741 atttactgca tttcctacta cgacttgaaa cattcctcca gcttcgacta cagtgatcac
    31801 accttctagt tgctctacct tctccttatc tgccttctta ttatctttta gggtaaagcg
    31861 taaccttgtt gcacaatggt aaacattgct gacattttct tcgcctccga caaatcggac
    31921 aatatctttc gctaattgtg aatagtccat ttttacttcc cccttgttaa ttaatgccgt
    31981 agcaatcctt cttacaagat aagtatagtt cttagaaaac ggtttctcca ttcaattagt
    32041 accccttata atagcgaacg aaactgtaca atagtgtgta tggtttactt agccgagcaa
    32101 atcactcact gcttgaaaga aaacaagtat atcgtttctc ttatagtcaa aggagacaaa
    32161 aaaaacacgc ttgatcttcc tttgctgatg tataagtggt atctcaagct gatactctgt
    32221 gatttttgtt tctacttggc ttttgataag acagcacaac aaagtctcgg catttaacgg
    32281 aagcataact aggtcagaac taggagagat tagttgctga atggaatcta tctttttatg
    32341 gatttttatt tgattttctt ctttgtagta atgatcaagc agttttgttt tctttttttc
    32401 ttcaactatg gtcagctttt cacgcaaaat ctgataaatc tcccaagatt caaactcatc
    32461 tagaacacta ggtagataaa tcaaatcatc ccactcaaaa ataagctctt gctcagtagt
    32521 caaaattgca tcatattccg ttattttgtt tgattgattt ttgaaaagat acactggttc
    32581 tatccggcat tctttgatcc acatgttcat actttgttga atctgttttt ttagtgtatt
    32641 caaaatacta attggctggt tactaacaat cagtatctcc ccttgttttc gaaaaggatt
    32701 caacgcttcg gcaagataca aaacaaaata agcgctttca gctaaattgg gacgtaattg
    32761 aaaatgtatt gggaagaaac gccgaattaa ataaatttct aaaggataac gggagatgat
    32821 cttttgtaca aaatgattca tggtattatg tccggcatcc atcctcgttt tcatttgttt
    32881 gatatgggtt agcaaaaatt ctgtttcatg gaacaataac gaaacaggaa gatctaaaaa
    32941 acgaataatc gtttgctcaa atgctcttag attcttctct atttctttgt ttttttcatc
    33001 attaatcatc aaataattta actctaatag tcgattttca attgcttgtt tttcttgatt
    33061 ggaaaaagaa tagggtagtt ctgatgataa cttttttgtg agggtctcta ccatcggaat
    33121 aaataatgta tacctgtttg ttttttctaa aagttcttct cttcgaagaa tggtcaaaac
    33181 gagataccga gaaaatcgta tgaagtcctc tcctgaaaca atataattat atgatataag
    33241 aacctctttt aatgtctttt ggacagtcgg catgatattg gaaaaaattg tactgatttg
    33301 ttgaggcaac cttgcgtgtt ggatcaccgt ttctgttcca acaagagata agaaaattct
    33361 ttttgaattt tcttcagcaa tgatttttag tccttttgtc gacgaaactt ccagttctaa
    33421 tttaggattg cgctcgatcc aacgcaaaat cgtttttatt tgtaatgata cggctgtttt
    33481 tgaaagaaag aaaatgtctg ctatcttttg catactgaca tacccttttt gaaacaataa
    33541 gtacgtcaca atagttgaag ctcggaaatc catgctggaa tagattttat gtttcgtcac
    33601 ctcttgtatg atttgctgtt tagtttcaga ttcaattgat tctaaaaaat aacctgaacg
    33661 tttatttgac tggataatga ttttcttctc gtagatagca agcatatttt tgatttcagt
    33721 ttggatcgtt ttcttgttgg tacctaaagc ttcagctaat tcttcgcttg ttatccaatg
    33781 attcttattt cttaatacta caagaatttg gagttgtcgt ttatttaagt ccatttgctt
    33841 acacctcctc tttgttataa acatacagga aaaacctctc aaatgacaat agtcacttgc
    33901 agaggtttaa aaatcatttg aagaaagatc tatattcaag gtttttacca ttagactgga
    33961 taatttgttt ataatattca aaactatctt ttttcaatct cctaaagctt ccgctcccat
    34021 cattttgtct atccacataa ataaagccat atcgcttcct catttcccct gttcctgccg
    34081 atacgatatc gattggtccc caccataagt aaccaatgat ttggcaacca tcttttattg
    34141 cctcagcaat ttcctgaaga tgtattttta gatactcaca acgttttgga tcgtggattc
    34201 gattcaattc atctggtgct tcatctagtc caatgccatt ttcaactatg aataatggta
    34261 attcataacg gtctgtcaat tcattcatga cataacgaaa ccctagagga tcaattggcc
    34321 aactccaagg ttcgggagat ttttccttca aaaagggatt atcgaaacca tttgctccgc
    34381 cagtatctac gcgcatttct gcttcttttt tatagacagc gctacgataa taactaaatg
    34441 caatataatc tgccgtgtta gcttgcaagc actgttcgtc tccatctaac attactggtg
    34501 cacagtcttc ttccttccat gtcctgtaaa tataagctgg ataatgccct cggcacatga
    34561 catcggagaa aaaccatgaa ttgcgtttaa attgcagtga accaaataca ttctctggat
    34621 cacagtcata tgcataagtc gctaatgcag aaaagctaca cataataccc atcttagcat
    34681 ttggatcgat tttctttaat aattgagtag cacgggcgtt cgctagaaac atatgatgac
    34741 aggcttgata aatcactttt tttgttagtg tagcaatggg ttcaagttta tctctagcag
    34801 ggaatgtaac caatccagca gctgcataag gcatacgaaa agcattattg atctcattaa
    34861 aggtcatcca atatttcact ttcccttggt agcgttcaaa gacggtctgc gcataaaatt
    34921 caaaaaagtc gattaatttt cgattttgcc accccccata ctctcctacc aatgctaagg
    34981 gggtttcata atgactaaga gtaattactg gctcgatatc atatcgattt aatgtattga
    35041 ttagtctgtc ataaaatacg agaccagcct catttggctt ctgttcgtct cctcttggaa
    35101 atattcgtgc ccatgagatg cttgttcgaa aagcttttaa acctaactcc ttcagtaaac
    35161 gaatatcctc ttcaaatcga tgataaaaat caatcgcttc atgagaaaga taatggagtg
    35221 attcgtctaa agcaatctcc caaatctttt tttcttcatt ccactgaata ctaggatgct
    35281 ggttcatgat cccgataagc gtatctgtca cattaggtaa tttgccgtct tccaaccatg
    35341 caccttcaca ttgattagca gctactgctc cgccccataa aaaattttct ggaaatattt
    35401 cctgatccat aactatctct cctcgctatc aacaatttct tacttgaagt atagctcgat
    35461 tcatcaaaac aagatattct aactatttcg gcagaagaga agaaaaaaat tgaatgctac
    35521 tcttcatttt taatatttat atataacaaa tacataagac gatccccgaa agaaaacact
    35581 cgttaaccat ctaagcaaac gatttatttt acttccaaca caaatgcttc ataaggtcgt
    35641 aaatcaactt tgccgtctgg taactgtttc agaggataat ttgacacaat aacccttaaa
    35701 gcttcacgtg gaacagtgaa cgtctgcttt tcactggaca tattacaaat aacgagccat
    35761 tgctggtctt catatatgcg agaataggca aagacatgct caggcgtctt ttccactaat
    35821 tcatagtctc cccagacgat gatgtcatgt tttctacgca aatcgatcaa tttcttgtac
    35881 atataaaaaa cagaatcttg atctgctaac gcttcttctg cattgatttc tttataattt
    35941 ggattgactc gaagccatgg aattccagta gtaaacccag catgttcacg ggcgttccat
    36001 tgcataggcg tcctagcatt gtctcttcct ttcgcattga ttgattccaa gatttcctct
    36061 tttgtaaatc cagaagaaat cctttcgtta tacatattga ttgtctcaat atcttttgct
    36121 tccgcgatat catcgattgg gcaattcgtc atgcctaact cttctccttg atagatataa
    36181 ggggtgcctt tcatcatatg gagcaagata gcaaacatct tggcactttc ttttcgatac
    36241 acatcgtcat ttccccaacg agaaacaatt cgcggaagat cgtgattatt ccaaaataaa
    36301 ctattccatc cttcatcacc taaagatgtt tgccatttgg ataatacttt tttcagttca
    36361 gagatactca agggtttcaa atcccatttg tctttgcctt cttgctgatc taatcctatg
    36421 tgttcgaatt ggaagaccat ggataattca tttcttttag gattggaata aagtttagcg
    36481 atttcggtag ttgctcccca tgtttcgcca acagtcatca gatttttgtc accaaaagtt
    36541 gcttgattca tttcctgaag atactcatgc agtttaggtc cattacttgt gatttcttta
    36601 tccggttctt tgccaatcat atcgatcacg tccatacgaa agcccccaat acctttatcg
    36661 atccagaaat tcatcatttg ataaatctct tggcgaacct gtttattttt ccaattcaaa
    36721 tcaggttgac gcttgctata caagtgcaga aaatattgcc ccgttttttc atccaactgc
    36781 caagcagaac cactgaatat tgagcgcaat ctattaggct cttttccatt gaccggatca
    36841 cgccaaatat aatagtcccg ataaggattt tctttatttt tacgggcctc aatgaaccac
    36901 ggatgttcat cagaagtatg attcaccacc agatccatga tgatacggat atttcttttc
    36961 tttgcttctt caatcagttc ttccatttct tccatcgtac cgaattctgc catgattgct
    37021 tgataattac tgatatcata cccattgtca tcattcggag actcgtatac tggagaaagc
    37081 caaattgcat caatacctaa atacgccaga taatccaacc tggaaataat cccccgcaaa
    37141 tctccaatac catcttgatt tgaatcttga aaacttcttg gatagatttg ataaacaact
    37201 gaatggtgcc accatttttg ttccatgatt ttcctcctat aaatggatgt ttctattttt
    37261 tgcttaatca tttattttag tctctctcac taaaaattgt aacgctttca aatagctatt
    37321 gcaccttgtt atcggtaaca gaataaaaca aaaaaacagc tgaggaaatt ccctcaactg
    37381 ttttttgaaa ggattgattt atgcagggaa gaatgctgat ttcacaactt ctttataaac
    37441 ggtgttcact cagctgtccc ttggtattag ctcaaaaaac ggcaaaatat gaggaactat
    37501 tttttcgttt ttttctctaa tactcaggcc agaacactga tttcacaacc tctttataaa
    37561 cggtgttcac tcagctgtcc ctcggtatta gctcaaaaaa cggcaaaata taagaaacta
    37621 ttttttcgtt tttttctcta atactcaggc cagaacgctg atttcacaac ctctttattt
    37681 cacattcaat atccacatga atcccacaaa tacaaagaac agaccataca tgattggaga
    37741 tacttctttt cctcttttag ctgctaacat ggtaattgga tagaagataa aacctaatgc
    37801 aatcccatct gaaatactat aagtcaaagg catgccgatc aagatcaaga atgaagggat
    37861 cgcaatctct aagtttcccc attcgatttg tcgcaaagat tgtgccatca atactcctac
    37921 cataatcaat gctggtgcag ttacttgtga agtaacaact gatagtaatg gagaaaagaa
    37981 caagcctaac aagaagaaaa tccctgtagt aatagctgtg atacctgaac gtcctccaac
    38041 ggcgatacca gctgatgatt ctacaaatgc accaacagga gaagtaccaa ataaggaacc
    38101 cgcaagcata gctgttgaat cagaagctaa agctttccct acacgaggca tcttgttgtc
    38161 tttcatgaaa cctgcttggt ttgctaatcc aactaaagtt cctgctgtat caaagaaagt
    38221 cacaagcaag aatgtcagca ctacgaccca catttgaaga gaattgatgt cccctacatg
    38281 ttttaatgca actaaaaatg ttggtttcaa acttggtgct gcggcaacaa tttcagaagg
    38341 cattggaatc aaacctgtga ccagacctaa aatcgttgta gctgctatcc cgatgaagat
    38401 gccacctggt acacgccgaa caagcaagat agcagttacg actaatccaa aaatcgtcaa
    38461 ccaagtagaa ccaacgttga gtgatcctaa tcctactaat gtagattcat tcgaaacgat
    38521 gatcccacct tcgctcagac caaggaaagc gataaataga ccgataccgc cagaaatcgc
    38581 atatttcaaa tctgctggga tcgcatcaat gatcatttcc cgcagcttga agattgtgat
    38641 caaaataaaa atcaaagaag caacgaatac gccggctaat gctgtttccc atggaatccc
    38701 cattcctaaa caaacagaat aagcaaaaaa agcattgatc ccaagagcag gtgccgttcc
    38761 aattggataa cgggctaata cccccattaa aacacagccg atcgcactgg ctaatgcagt
    38821 cgcagtaaac actgctcctt catccattcc agaagcacct aatacagttg gatttacaaa
    38881 taagatataa gccattgaga taaatgttgt aaatcctgcc agtacctcac gttttaccgt
    38941 ggtgttcaat ttttctattt cgaagtagga aagtattttt tccttcatct tccatccccc
    39001 taaatccgaa cattataaat aaagtccgtt aaaatttcga tttattaata aaacaacgtg
    39061 ttttatcata tactaaaaca aaacataagt aaacaaaaaa ttcggatttt aagcaaaatc
    39121 cgaacgttcc atctgattaa atccctttat ctcaaagttc atgaacattc actttggatt
    39181 acaagaattt ctttattgtg ttaataaagt ttcttcattc tagtctcaaa aatcaatagt
    39241 gtgatttgat tttaccattt cgtttctgtt cttctttgat gaattctttt gtatcgacag
    39301 gattcagatt atttagtccg ccaaaacctg tatcgaccat ttttccccag ccttttgtct
    39361 tcttttttcc tgatttgaga ttctttgctc gaagcttctc actatgtttt gctcgccgat
    39421 acttcaaata accttctaat cttcccatta gatccactgt ttttcctcct ttttattcaa
    39481 tcagacgact cagcttttcc tataacaaaa taattgcctt cagggtcaga aaaattgaaa
    39541 gtcaagatgc cattgttatc tgtgatttct cctggttctt ccatgttctc gtagatagtt
    39601 tcaaattcat ccgtaaaaaa tactaaagaa ggcggtggtc ctaatacctc tggtgaaaac
    39661 ttctgcacaa atacttttgg gaatacgcct aattctaatt cttttgagat gctgagtaca
    39721 atactcttaa attcttctgg caattcgatt gtttcagaaa tttctgcacc aattttttct
    39781 acaaagaatc ggcaaatcat ctctacgtca ttgacataaa gcatcacacg tgttttttgt
    39841 atcatcattt gacccttctt tctatttgat tttctttcct gtctataaac tgattccatt
    39901 atatcacgaa agcgcaaaca ccttgtgttt gatactattc aagaaaaata aagcaatagt
    39961 ttctaggatt gctttcctcc acaaactttt tttacaaaaa aaaaaaacgg caatgaatgc
    40021 cgctcttctt atttcgattc ttttataatt ctgcttctga ttcatctttg gttagtgtgt
    40081 gtctaacact agttacgcca aatgtttgat cgattttttc cttcaacgct tcgattgcct
    40141 gtgcgccacg tgccgcttgt tcagcattga tggattcaat gaccagcacc gctgcttgtg
    40201 tttcttccgt cagcattgtt ctttgggctt cacgccagtt gagacagcga cagatcgctc
    40261 cttgttcatc atagtagatg atttcttccg gaagtgccgg agcatcggaa tctgcaccta
    40321 aaggcaggaa tgattcgcca ccttgggctt ttcctaaatg cagatttcct gcgatctttt
    40381 cgatatcttc tccgccgcaa ggtacgccaa aagacaagga aatactatta tagatatcta
    40441 caagcggatt gattgggaaa aattcccgtt cttgatttgc acgtttcagt aaagcttcaa
    40501 tagaagaacg tgcacctttt ttagtcttga atgtggtaaa tgcctgacgc cactgagcaa
    40561 tgacttcgtt ttggctgaat gtttcttctg taaggtaatt tcttgcttct tttgctgcct
    40621 cattcaataa ttgatgatac gtttcttcac tttcttttgt tacgtggtta tcgataccat
    40681 ttacggataa gatttcgatg gttgcttcag gaaatacttc ccagaaattg gtatccacta
    40741 ttactttttt catctgttca cacctcttga ttagaataat tgcttgatga atagatagtc
    40801 tgtttgggga tcatctccca tataaaaaac atgttcgctg tatttgataa atcctttttt
    40861 acgataaaaa gcgagcgcaa catcgttgtg ttcccaaaca ccgagccaaa tggctgtttt
    40921 ttgcttttct tttgcttttt caatagctgt atctagtaaa aatgtaccga taccttgatg
    40981 tttaaatttt tttcgtacat agattcgctc gatttctaaa aaatgatcag ccatttcctc
    41041 agattgactc gttcctgtat ttagtttcag gtaacccgca ggctcttgat cgatactcac
    41101 taggaaaaaa tcggtttctt tatcttttag ctcttctgta agtttttcta gtgcataagc
    41161 ttgtgtcaag taatcagcta aatgctccgg tgaattatct tttccaaatg tatctgtaaa
    41221 tgtttcgata ctaatttgtt gtagtaagtc tacatctgaa ggcgtcattt ttcgaatttc
    41281 gtatttattc acggcttctt cctttcaata gatcctttta ttgccttttt tgacaaaatt
    41341 ccaatcttcg ctaacattct tgcatacttt atttaaaaga tattccaatt gttttgcttc
    41401 ttcgtcagaa agaccattta atgcgactgc attggagtag ttgttttctc gaatgatgaa
    41461 aggatagatt tctgctcctt ttttagtcgg atagagtttc ttgatttttt tattttcttt
    41521 atcttccaaa cgttcgatca tcccattgga ttctaatttc ttgattgctc gggcagtcgt
    41581 tgtacgatct actttgatca tttctgctag tttttcttgg atgattccag gattttcgca
    41641 aatacgtact aaataaagat actgtcctct tgttaaatcg acttctttga attcgatatt
    41701 gctaatagaa tctagcgctc gggcaatcat tccgatgtct cgaaggattt cagccatttc
    41761 atcccacctt tattttgttt gtaagactag tttacctctt tttgttgcaa atgcaactat
    41821 tttcaataaa aaaatcaagt tcttttttaa taactagtaa agtatattct tttcttcttt
    41881 tacgaaaaag ctcaatcaca atagatctgg tttttgtttt ctaaaataag taactgtttc
    41941 ttcgttgttc gcttttctga tagctttggc aactcttcta tactaaaaaa tcccatgttg
    42001 gaagtttcat tattctcaat aaacttggcc tcttcaccgt ggatcgcaca agcaaaaaca
    42061 agtttataat actgaaaaag ctgtggaata tctttttgtt tattcgtatc aaaaactgct
    42121 cgcaactgag tagtttctac tgttaatcct gtttcttcat agacttcttt acgcacattt
    42181 tcttcaggag acaatcctat ttcagcaaat ccaccaggaa gtgaccattc tttcgtccgt
    42241 ttatcttcga ttagtaggac cttgccatct tttttgatat aggcacgaac atctactttt
    42301 ggtgttggat gtccttctcc aatcgttgtg agcgctttta tttcttcgaa agtttctttt
    42361 cctatttcac tgatcagttc caatgaaatc gtccggatct cctcaaaacg ctctttatca
    42421 aatacatctt ttccataata tagaccagct tgagaaagtg cgagtagtct tttgtattga
    42481 gccaacttct tgttcattct cttcccttct tttttagcaa atgtatgact atgtaatcaa
    42541 gtatagaaga ctggtttaat tacggcaaat aaatataatg aaatctgcaa taaataaaag
    42601 aatgttaaaa aactcgagaa caaaagacat tcgacttctc tggggctgtg aataaatggc
    42661 gttcaaaagc ttgcatctgc ggtaataagc cccaccaaac gaaaaatatg acgagctatt
    42721 cttgtctggc tgttcttatt aattgaggca ggacagcttt ttcacaacct ccccactaaa
    42781 cggtgttctc ctaccaactc ctcggtcaca agtcacaata ttcatcaaac atgagaagct
    42841 gtttttttga aattgctcct tgtgactcgg agctgaacga caggctcacg acctctcggt
    42901 aaacagcgtt caaaagccaa tttttcgata ttaagccatt tctttgttac ttattgtcat
    42961 tgatttcttt ttctaattta tccaatagtg gagtgtaatc cacttgagta tagtctgtta
    43021 catcaaattc atatagtgtt cctaactgga atttgtcata ttttcggtcg gcgatgatct
    43081 ttttgaatgc ttctttcgta atatgattgg tccctttcgt tcgatcttcc aatacgtcac
    43141 catagtgata atgatccatg atatagctca aatgacgatc tgattcacga tcacgtttgt
    43201 accgacgttg acataacact tcaaagtctg ctgttaggcg aatcgtgatc acctgatatc
    43261 cgtatctatc tgctaattct tggagtcgtg ctttttgctt atcactaaaa ggatactcgg
    43321 acacaatcac tttctttcct gcttccatat acaaatcaag tgctttgtaa tagaaaggat
    43381 aaactttcgt cctttcttgt tccgcccgtt cttccaaatt gttaaacccg atggattctg
    43441 catagtattc cttgatttca tcgagcgtga tggcatacat atctggaaat cgttcccgta
    43501 gtttattcat aagatatgtt tttcctgtac caggactacc agccagaaga atgaaatact
    43561 ttttcatctc atttatttcc taagttaatt ggactttcca agttcataaa cagtccaatc
    43621 ttatgcttga caatttcttt tactgcttca tttgctggaa tcacattatg gcgaagagac
    43681 ttgttgattt ctgtttcact aaaggtttct ttagctactt tcgtccagtt gactaaaagc
    43741 tccgttccaa cattgaattt gcggatcccg ttattgatca attcaggata atcttcttct
    43801 ttcacacctg ttcctccatg gataactagg gggacatcaa ctactgcatc gatttctttc
    43861 agcaagggaa tgtttacttc tgtttttgat ttgaattgtc catgattcgt accgatagca
    43921 atagctaatg catccacgcc tgtagcttct acaaatttca ctgcgtcttc cggtcttgta
    43981 tataccttgt cattttcagc gacatggatt ccttcttctg taccgccgat tgttccaagt
    44041 tctccttcta cggaaacacc gtgcgcatgg gcatattcta ctactgcttt tgtcttcagg
    44101 atattttctt tgaaaggcag atgggaccca tcatacatga cagaagtata tccgctgtcg
    44161 attgcttctt tgatatcatc aaagtctcgg gcgtggtcta agtgcaagac tgcatcaacc
    44221 atttcttctt ctgcaatagc tgtcattaca ttgactaaca catgcatgcc gatatattta
    44281 gcggtatcga cacttgtttg aataatgatc ggcgaaccca tttcttttgc tgcgcctagc
    44341 atagcaggaa gcatttctag gttatgcgta ttaaaagctc caactgtaaa attcaactct
    44401 tctgctgttt ttgttacttc tttcaatgtt gtatacatct tttattgcca ccttatcttt
    44461 ttatttgttt cctttgatcg ttacttgttt tgcaatttgt cgcatctcat ccattttgcc
    44521 catgtagtaa tcgatccctt gatcatcacc agcacgagca gcatctgcac gcttctggtt
    44581 atagaggctc atcagttttt tatacccatc tccgatcgtc tgtttatttt ccaaactctt
    44641 ttctaaatat tggatggctt gatcgttcac atacaattca gaaaatttaa gtccaacttg
    44701 ttcataaagt ttcgctaatt tatgcttatc ttgggtagtg gaaatctctt gtttcaattg
    44761 tgtaatctct gattgaatct tttcggtcgc ttctggcgat agggcttttt cttcttttgc
    44821 cagaactttt ttcggttctt tttctttttt cttaaaaaat ccaaacatca ttttccctac
    44881 tttcaattaa aatgctttca aaactgggct taagatccag gcatataaga gtgctgcggc
    44941 tactgtatct acatcggttg ctgtagcatt gatgaatccc atcgaattaa agatcgttac
    45001 tagcaaagcg ggcaataatg taataaagaa accatgagca ataccgccaa tgatcgctcc
    45061 tcggcgtccg ccaactgcgt tgccaaatat ccctgctgtc cctccggcaa agaaattggt
    45121 tagcatacca ggtaaaatca tcgctaagcc gaacatgggc aaagtaaaca tggcaataat
    45181 cgtaccaatc gttgtcgtaa tgaaacctaa aatgacagca tttgggctat aaggaaagaa
    45241 aactggacaa tctaaggcag ggatcgcatc aggaactaat ttcatagcta tccctcgaaa
    45301 tgccggcacg atttcaccta acagtagacg tacaccagct agcaatacat acacacccac
    45361 tacgaattgg atggcttgta aaaaggcaaa catgatatag ttttgatctc ctgataattc
    45421 agaagcgaat ggttcgcctg caaataaaac agtgatgata tatagcggga ccatcacgac
    45481 catgacagac aaataagtat cttgcagaaa ttcgaaatgt gtcggcaact tgatatcttc
    45541 gatcgacttt ttattttcgc ctttctcacc aaaaagctta gcaacaccgg cttcaaataa
    45601 gtacccgatc gtacaaaaat gacctaaagc aatatcattt gatccagtga ttttacgaat
    45661 gatcggttgc gctactgccg gcatagcaat ggcgaaacaa gctccgataa aaccaccgac
    45721 caagatcaat acgatccctc gcagaccggc aaaatagcca aagactgtcg tcatcgttgc
    45781 catccataaa attgcttgac cagttaaaaa aatgtacttc cattttgtga aacgagccaa
    45841 aataatattg aatacaaaaa tggctaagaa cgtcagtgca atatctctac ctaaacccaa
    45901 atcattcatt gcttgaccat tgatagattc aatagaaggg atgatccctt gcatatgaaa
    45961 tccttcagta aagattttcc cgaaataagt taaacttcca acgatgatgc tcgaaccggc
    46021 actcaacact tgaaatccta ggagtgtctt taacgtgcca gagatgactt gtcctgctga
    46081 tttcttctgc agtagtagac ccaacatagc aatcaatgca atggtgatcg atgcctgagt
    46141 caaaatattt tcgatgataa aattgataac gcccatgttt ttaccctctt ttctttttgt
    46201 atttagataa ctcccttatc ttgcagaact ggggtgattt tttcttcaat ttcatttttt
    46261 gaaacaatat ttttcaaata gatcatcgcc gttttaggat caactgcgaa cttttcgaac
    46321 tgcgaacgaa aattttctgc tgtgatgatc acatctggtt tcatggaaac tgcggaagaa
    46381 atatcacaat ggtccagttt ggcttctact cctaatttgt tcaaaacatc ctcagcagac
    46441 atctgtgctg caaaactact tcctaaacct gcaccgcata cgaataaaat gttcaatttg
    46501 ttcatctttg ttctcccccg tatcatttat tatttctcaa ctactgaaaa aagcagttgt
    46561 ttgaaatctt cgatatttgt gcattcaatc aatcgatcca acttctcttc gttgttgatc
    46621 aattcaatta gttctttcat gatgttcaga tgcgagtagg agtctactgc tgccaaacaa
    46681 aaaataatct ttactggatc catttcagga ttaccgaaat cgattggttg ctcaatcgta
    46741 gctacgctga ctcctaattt attgacccca tcttctggtc gggcatgggc tagtgccatg
    46801 tgtttgccaa tcacgatata cggtccgtaa tcattcactg cacgaatcat cgcttcgaca
    46861 tagcgctctt cgacaatgtt ttctttaacc aatggtctag cagctagttc aatcgattcc
    46921 tcccaagtag tggctttttc tttgatcatg atattgctgt ccgtcaaaat atcttttaac
    46981 ataggctgga tctccctttt gttgatttct aaattgtttg tttcgaataa tttttctaac
    47041 ttgccataga tgattcgatc tactcggcca ccactttctt tcatgacatc tattatttga
    47101 gtgaataact ccgtcgattc atgactgtta ggctctaacc gctgtagttc ttcatttctt
    47161 ttcaaaaagt cttctataac caagatattc gtctcagtta aaatcggatc gacaactaaa
    47221 agcggcttat ttcgataatt caattgaaag gtgctgaaaa ccagattgac atccaattta
    47281 tctactaatt cgatttcttt tgaacttaaa actgcagtaa tctcgatctg aggaaacttt
    47341 tctttcagat tctctgctaa cagatttcca gtagccatcc catggttaca gacaacgact
    47401 gatttataaa cataattcaa gtctcgcttg attacactgg cgatcgtcga aaagtggatg
    47461 acaagaaaag ctatctcgtc ctccagtatc ttgccaccaa ttacttcttc gatcgtagga
    47521 acaaaatgag tgactgcgcg gtagattggg aaataatttg ttcggatgtt ttctttcaat
    47581 ggattcatga cttggatatg attcttgatt cttacaatca gtgcagccat gtgcttatag
    47641 agattttcga ataaagcttc ttcctttaga tggaaaggta cgtgtgtctc gttctctaca
    47701 aattggatca attggatcgt tagtaattga gcttgtaccc actcgattga attggagata
    47761 tctctagcat tgaacgtctc aatagtgaaa cgaagataat ttctttctac ttgcgggaca
    47821 ttgtccaatt gaaattcctc gatcatgtgg tcaataaatg cagaaaagct actgtctttt
    47881 atttctgtat ttttccaatt aatggcagag ataaattcat gtttcctgat tcgctggatc
    47941 cagatcaatg taaaaagcag gatttgtttt ttataaatat catcttcttg tttcaacaac
    48001 ttattggaat agatctcctg tatttttttg atttccacca aaggaaaata atggagaaag
    48061 attttttgag ccggtgttaa ttgtgcatct tgattcataa agaagtcggt cattcccaga
    48121 ttctgattga taaaatcata gatcatcgta cggatagatc gttctgaacc tttataaacc
    48181 agtccttgct ttccataact gacgatctct atcccataac tgaccagtgt tgctcgtaac
    48241 cgtctcatat cttcatctaa tgtactttta gagatttgaa attcttcttc ttttttgttt
    48301 agatggatcg ggattcggct gtaagcaacg gctaaaacta gatcaaaaat acgttcgcgc
    48361 cgattcaaaa agccgtcttt cacatctttt atttcagttt ctaatcgagc ttgcttttcc
    48421 ttttccagct tcaacaaaaa aactttgttg cgaattgtac ggatctccgg aagcttttta
    48481 ctgctcaacc agatattcgc ttcaagaact tcctgacgga tcgtccgctg actgacattg
    48541 aactcacttg ctagatgttt tgcttttact ggatattggg ctgtcatgat tctgtgaagt
    48601 atttcaattg ttcttgtttt catagaaatg atctccttct gatttcaagt atatggttcc
    48661 gcttacataa taaaaagtag atatttctgc cacttgaagt ggaaagaaag taagcaaagc
    48721 aaacaaaaaa taagccatat gctttttcat ctcctcaacg aaaaagacat acgcctatta
    48781 ttacaaaaat tttgcccatc tatacaaaaa agaggttgtg aaaaagctgt cctgcatcaa
    48841 gtaataagaa caactaaact aaaatagtgc ttcctatttt tcgtttggtt gttcttatta
    48901 tcgcagatgc aagcttttaa acaccattta gtggagaggt tgtgacagag atgttcagcc
    48961 ttgagcgata agaagcgatt ttgaaaaatc gcttcccata ttttacaaaa tcgtgcctta
    49021 ttgccgatag gctatctctg gcacactgtt tattcagaaa aaggagctgc gacaaggctt
    49081 ttgtcacaac tccgttcata aatatttcct gtcaatccag cataatcggc tgaataaaat
    49141 cggcttgcca tcttttttct ttctcagaaa aatcaaaata ccatatctga gaaaaatact
    49201 gtcgttgtgt cttacttcct gagatcaatc gcccatcttt tcgatcgact gaataatcac
    49261 agcatctgaa atcaattcgg atggcaaaac tattttcgtg gatctgtcga taatttccta
    49321 cttcctgaat agttattttt ttagtatgat ttcttacacc gtctgctttg tttttttgca
    49381 gaactttctg atgttcggca aacagcttgt ctgtataaca gcttctggct ttttcaggag
    49441 tttcttcttc ccaagcggat tggatcttca tgaatatctt ttggatttcc agaaactgtg
    49501 ctttcttttc tttagccgtt cctgacatgc gggacaatat ctccgctttc agccttttct
    49561 tttgtctttt attcttgacg atagaaaaag cagaaacccc tcctactgac aaaacagcta
    49621 atgcaagaaa tcccgtttgc tcataactag aaggacctgt acctggacca taatacccat
    49681 aagaatctct gccgtaagaa cgactgccaa aataactgcc cccatatgaa cgaccaccaa
    49741 atgaacctct gcccgaactt cgaccgctag atgtacctcc accatgacca cccgcgacag
    49801 cgaatccttg ggtcggcaga gcaaacaaac ataggcccac taacaaaatg attgctaaat
    49861 aattgatttt tttcattgtc ttcctcgtta tcttttatta caaaaaacta tttttatgat
    49921 agccttgcta aaaggaagag tcaataacca gatgcataaa ctgttttttt agcaattttg
    49981 tcttatttag tcattgagta acatcggaca aatgtatgtg tcggcagtcc ataattcctc
    50041 tatcttcagc atgaacaagc ttgcagacaa gagatctgcg cttcctccag gagagatttg
    50101 gttttcctga caaaagtgca acagtgctgt aaatggacga tttggtaaag cactttgact
    50161 agcagcatga gcaagttttt gcatttccac taacgttttc tgatcactgc gatgcaatac
    50221 gcatgtgtca tctagcgaac tcatcaaaac taacaacata tgcatttgtt tctgtacata
    50281 tgaatcatta tctagctcct tagctgcttg gattgcaagc ctgatatgcg gataccctaa
    50341 tatcgcttct tcatatgcac catttacgcc atatttttgc ttcaccctat gaccatgagt
    50401 ggttttttta tgacgatatt ttgtatcagg aaaagcagca agcttgctga catcatcaag
    50461 tattttcgat aaagaaaaag gctgttccat actgattttt tgtgcaatga cactgatcaa
    50521 tagccccatt gcccaaatcg ctcctttatg ggtattgatg ccatttgtag caatcagcat
    50581 tgccttttcc gcttttcgcc caatctcagc aattctttct cttaactcct gatcaatagc
    50641 atgatgccat gctatttgag ccatttcttc aaaaaaagga gttagcgtct tagcagactt
    50701 aagaaataaa gcatagtcca tatcggaatg tgcacccgca tccagtgcat ccactaatcc
    50761 aggttttgga cttaatgtta cttctgcgat caatgcttcc tctaccaacg cagcgagttg
    50821 ctgagtcact gctttttttc gttcgtcatt tatcatgaca ctcactcctt cttcctacta
    50881 ttacttgctg aaaccttttt tgatagctac ccaaatgata tgaagtaacc agcttaaagc
    50941 aaatgcacaa cttgccgtca ttagataagc tacaagaatc atgactcctg aaggtaactg
    51001 cagccaacta tctttaaaca ataataacag tacttggaaa acaaatacat tggctaaata
    51061 cgtacgataa gcataaacag acaaccattt gaacaatggc aattttggcg aatcgtttac
    51121 aatcattttg gaagcgccta tgaagacagc aaagatgatg actaaactgt ataaactttg
    51181 gatcgtattc aagtaaggtg cattcgcaaa gctcaagtct cctggatgtg cgagcaatga
    51241 acggacagaa aagaacgcga taactaaggc aacaggtaag aaaagataac gtactctgtc
    51301 caaatattga tagacttttt cgtgataaac aaatgcggct accccataaa tcatgaaact
    51361 agcaacaaac cgatctaata agtaccagct gtcatgataa ggcccagtca atacttgtgt
    51421 ttgatagaac agataccatg cgacatataa aatcgttgaa ccaatcaata ctggccatac
    51481 atatttttta ttagcaaata ctttgtaacc gagccaaaca aaaaacggca tgaacaattg
    51541 gatctgcagc atcataacgg tataccataa atgaggcgct ccatcacctg tgacatattt
    51601 cagtaaaaag cttgcagtat ccgtatacgg agtttgttgt tggactgctg gaaatagcca
    51661 aagataagcc gtcgtccata gaagatacgg aacgactagt tcaaaaaact tttctttcaa
    51721 aaattcccaa taagacaaat gctgacttgt ttttaccata ttataaacaa ttgcaaaaat
    51781 aaacattggt gctgtgtatt taacgatgac gtaaaaggta ccaagaaaag ctgtatttgt
    51841 tgcgctgtgg ctgtttgcca aagcaaagct caatatcgtt tgcatcataa cagccgttac
    51901 agctgatact ttctggaagt cacttacgtt gattttgatt tttttgtttt caaacatctc
    51961 tttctctctc tttttcttta ttaccaaagt tctttggtta atttaggtcc ttcgactgtt
    52021 ttcaatagaa tttttttctc tggctctctc aaatactctt ttagtgaaaa accgccttgc
    52081 cgagtcacta cttgggtatc gaattgctca aaaaaagcag gatgcgattg aattacctga
    52141 attggcaatt caatcggtga atttgcgtac agcagcaaat caaaatcact ggtttctttt
    52201 actgccggaa tcccagtcga taattcaaat cctaagctgc cgccgattcc ccatttacat
    52261 cctgataata tgtttctagc agcatgaaat tgctgataaa caggataatc catgattttc
    52321 tcttgtctat acgattcttg ttcgatgatg tcccacggat gaatgacttg tttgacggca
    52381 tgttttgaga tttctgttga ccatctctga ttgcgggcag gtccacgtaa accaatcggt
    52441 agtatttctg cgtgatccgt cgacgcacga cgaactgttc cgaacatctt ttcagttaga
    52501 acgacccatt caggcaaggt gaacccttct atttcatgtt tatcaaaaag gacaatatca
    52561 tgtgcttgaa tcatttttta tgcatcccat tcttcgtcca ttttggcacg gactttattt
    52621 gtttcgacac gtccgaatgt tttggctgtt tcgtttgtgt atcgatttgt cagcatcgtg
    52681 tcatttgtat tgcggacatc ttgaatggct tcattgattg cttcgatgac tttgctgaca
    52741 ttttgttctg aagcagcttg gtcggtgata tcttctagga aatgatagat cgctcctagt
    52801 tttgtataat tattgatatc gtaagcgata gctggaactt tttcagcagc tgcttcgatt
    52861 tcagcaaccg aacgttttgt cacacgtgca gcactttctt tagacatcgc ttggacggta
    52921 atttctttat tgtttaaagc aatcaagcgg cttgattgta atccatgtgc taaaaatgca
    52981 cctgaaatag catttcctgc aatcaatcca ataactggat gtccagcttg acggagtttt
    53041 gcataagcag cagcactatt agccaaagcc acatggatac cgatcaattc ttcgttatag
    53101 ccgtatgctt ggcttggtac atcgatcaca acgatgattg gccgtttcac ttttttgtct
    53161 ttatcttctt cgtaaacgtg atccaaaatt tgtcccatgc ggaaaccttc aactaaacca
    53221 acttcaccgt ttcgtacacg gtacatcggg tttttaggat caggtacgat tgatgtcacg
    53281 atggcttctt ctatgaattt acttgattta ccataataca cagtactaat agaagaagtt
    53341 ggatttttca tgccagttag tgccgcaaac caatcgtaac caacacttgc tgcagctggt
    53401 tcatttcctt ctgctacttc tggtaagtca attgattttg cttgatggtt agcaattgct
    53461 tcattgtaat cgtcaatggc cattggtttt gaaagatcta aagaatcaag taatgataaa
    53521 taaaatgctg agctttcact gcgatgcgtg tcttttcgtt cgtccattgc tgcgatgaca
    53581 gcagctttga ttgcttctac actatcagtc actaattcat caatgatttt tgattctacc
    53641 cgttgacgag atcctaatgt attccaaatc aagtctttgt cactagaatc aaattctcga
    53701 acgcctgctt cttgttcaat aacttctggt ccattcaaac cgactctcgc tttttttgtt
    53761 gcgattaaat aactcattaa tgctgaagta atcgacatac cgccaaatga tcctacacgt
    53821 cctggaatca aaccgatgac tggtacatat tgttttagag caatgattac atttccgatt
    53881 tctgaaatag ataacagtcc gtagtttgct tcttgcaaac gaacaccacc tgtatctagt
    53941 acaatgactg gaaagatctc atttcctttt ttattttctt ctaacacatg ttcaagtgca
    54001 gcaacgattt ttgcaccgga aacttcaccg ataccgccac cttggaattt tccttccata
    54061 gcgattacta ctgcttgttt gccattgatc gtgccacggc tgacaacgat cccgtcatca
    54121 ctttcaggga cgattccttg tgctggtaaa cttggcgcga tgatttgatc gaatggatca
    54181 atcaattcac gtccattgtc aagtagagcg ttcactcgtt ctctggcgtt taattctaca
    54241 aaactatttt tcatctgaaa gttcctccat tgcttgcgtc aatcgtaggt acacaactcc
    54301 tggtgtagca ccgaagtcat tgatcacgat atctgctgaa attggataac gatcaaaaaa
    54361 tctcgttaat acattgttcc atacttcgcc gaagccatcg cttcctgtac aaatttttac
    54421 agtagattct gttttttctg ttggaaacat caagatttcc aaatctcctg aaccgaccac
    54481 tcctacatgg attttcttat tgatcggctg tttcgttgga tatttataag ctaattgttc
    54541 catggatctc tccccttttt gtaaaacaga gcatacacgt tctgcttatt cgtctttctt
    54601 cttccatctg ggtaaatggc gacaaaaggt ttttctcgcc aattttatga ccaagttctg
    54661 aatttcattg gtggttcata caggccatct gaccattcga tcaaatcatc gatggtttgt
    54721 gcagctaata atgaacgttt cgcttcacta cgtttgatgc ctagatcttc tggtaatttc
    54781 acgatgccgg ctgcacgcaa gctatctagt gattctttcg tgctagtcat tccaagaggt
    54841 gtaacaccgc caattgctgc aattgctgct tgtcgttctt cttttgtttt tgctttgtac
    54901 aaataagcaa ttccttcttc tgtcacgata tgtgtagtat cttcgctata aatcatcact
    54961 ggtgcattgg ctaagcctgc ttgtttttga acagctagtg catctaattc ttcgacaaag
    55021 actggttttt tatttgaacc atatgtttca accatttgaa caactaattt ttggcctttc
    55081 cctaatggat catcattgtc gcgaagtgat tgccatgctg gtgtagaatg acgacgaccg
    55141 ccaggattat gtcccatgtt aggcgcaccg ccaaaaccag ataaacgtcc ttttgttacg
    55201 gtagaagagt ttccttcgta atctaattgc aaggttgaac cgataaacat atcgatggca
    55261 tattgtcctg cagcttgtga taatgttcgg ttactgcgca tcgtgccgtc tttaccgaca
    55321 aaaaagacat ctgggcgagc agcaatatat ttttccatac caacttcgcc accaaaacta
    55381 tggatgcttt caacccaacc tgattcgatg gctggaatca tcgttggatg tgggttcaat
    55441 acccagttct tcgcgatttt cccttttaac cctagtgatt caccataagt tggcagtaac
    55501 aattcgatag ctgctgtatt gaatccaata ccgtgattca aagattgtac gccgtgttct
    55561 gcgtagattc ctttgattgc catcattgcc attagaattt gcaattctgt gatgttttga
    55621 ggatctcgtg tgaataaagc ttctaattga taaggtttat ctgctttcac gacaacgtct
    55681 acccagtcac cagggatatc tacacgtggt aaagaatcga caacttcgtt gacttgaacg
    55741 atcacgatac catctttgaa agctgcagct tcgatgattg ttggtgtttc ttccgtattg
    55801 aaacctgtgt acaagttgcc gtctttatct gctttatctg cagctactaa aacaacattt
    55861 ggaatcaaat cgatgaataa gcgtccataa agttccaaat aagtatggat atcacctaat
    55921 ttcattgtgc cgtcttcaat catttgggcg acacgcaagc tttgtgcacc agcataagag
    55981 aaatcgatct tgcttgcgat tcctttttca aaaatattca aatgttctgg tcgtgagata
    56041 ctagacatga tcaaatgcag atcattcact ttttctgcat ctactttttc aagactttct
    56101 gaaagaaaac ttgcttgttt ttgattgtcg ccttctaata caactttgtc gccgggagca
    56161 atcagttttt ccattacttc tacgattttt tcagtttcta catacttgcc attgagcaag
    56221 ttgctgattt tttccaatcg ctgcgctttt ttttcgcgtt tagaagccca gttgcggttt
    56281 ggtttttcca taattagtca tctccatttt tgatatagaa acgtggcgtc atcaatacca
    56341 tgttggtcta gattgatcgt acggatccct ttatctacac caaattttgt atgaatcagc
    56401 tttgtcaaag tattgccggg gggaaattca atcgtcagat ccattcctaa ttcttttgcc
    56461 aagtccatca tttggttcca ctgcacaggg aaagctaggt tttccaacaa atctttacga
    56521 atctcttctg cgtcctgagt ggctcttcca tccgtatttt ttatatacaa acatctagca
    56581 tcagatagat ggacagactg catatacggc ttgaatttct tgaccgtatc attcatcaag
    56641 agacagtgag atggcacagg gactttcagc agcttcgctg tttgcgcttg gtgccgtttg
    56701 gcttctgcca acgtaacgga taaaccttcg atactcccag ataacgtatg ttgcatcgga
    56761 caattttcat ttgacagata aactggatgg tcaggatcaa atgtttttgc tacaatttcc
    56821 tcagcttcgc ttctcgtcac acctactact acgcccattg cataaccgct tggataagct
    56881 tctttcatta agactgcgcg gtgatagact agttccgctg cattctcaaa tctcaaagat
    56941 cgacaagcaa cagctgcaca aaaagcacca atcgaatgtc ctgctgcaat atcaggctga
    57001 ataccgtttt tttccatttc atctaaataa aatactgctt ttgtcagtaa tgctagctgg
    57061 ataaaaaccg tatcttgata gttttcttct ttatcaacta accgataccc cgtcacattt
    57121 tctaaacgtt ccatgtacat ttcagggata tccgctaaca tccctaactt ctgactgcct
    57181 tgtccaggaa ataagaaacc tgtactcatg agcctcgcct cctcagcact tactttttcc
    57241 actgctatct tagtcaattt ctgctataat aaaaaatgcc aaaaaaagaa aaaaccataa
    57301 caaaaaggta tggatgggat ggataattat gaaattgctt catctgcagt attttaaaaa
    57361 agtattcgaa acaaaaaatg ttacccaagc agccaaagaa ttatttattt ctcaacctgc
    57421 cttgagccgt gcgatcaaac atttggaaaa cgaattaggt gtccctttgt tttaccatag
    57481 cggcagaaac attgagacga ccgtttatgc agaagaattt tatccatatg cagtcaagac
    57541 attagacaca ttagaagaag gaatcaaatt attgtcttcg gtcaatgaga agatcgttac
    57601 ttccgtcata ttgtatttag aggtagcctc tgtttctatt ccgaacttgg tccgaatttt
    57661 tatggaaaag catcctgata tccaattaac gatcatgcaa catcatttac cagatgatat
    57721 taccgatcct gtactgtaca tcacttcgga gatgaagcct ggcatgacca atattcctat
    57781 catgaaagaa cctgtttatg tcgccattcc taagaaacat cctcttgcta aaaaagaaca
    57841 tctgcaatta gaagatatcc aacgcacccc catcttgatg ctgtcaaaga gcaacgcctt
    57901 ccgtcataca ctggatgcag cacttgaagc gaaaagtatc gaattgacga tcagttcgac
    57961 gacagatgat cctgcgacat tacgttctat tgtcaagcaa ggattagctg ttagcttttt
    58021 cccaaaagtt tcttggtctt acgataaaaa tgatcctttc gtcttgcggg aaatcaaaga
    58081 tttgcccttg acacgaacaa tttatttatc ttctagcttc acagaagatc atcctttgat
    58141 caaaatgatt gcaaaaacga tgcgggaatt ttatttagga aaataaaaaa aaaagagctg
    58201 ggacaaaatt cttgtcgcag ctcttttttc gaacaaacgg tgttccagaa gtaatttctt
    58261 agaaaataag ccgaaatttc ccaaaaattt gagaagcaat tttcgtaaat tccttcttat
    58321 ttcttgaagc taaacatttc tgtcacaacc tcttctctat tgtcctagac attcattgcg
    58381 ttgatttaaa acttccatca atcaaatctg ctagttcatc tagcttttct attttacgta
    58441 gatcaataga gatccaatgt tctttattca tgtgataggc tggaaaatac ccctcttttc
    58501 ctcgcaaaat tccgccaagt tcaggatcga ccttcaaatc aagaatattg atgatcctat
    58561 ggtcacttgc atcatcatag attttttctt ctggaacaga catcaggacc ccaaaccatt
    58621 ttttgctttt ttgatgtctg aatacgcaat aatcaggaaa ttttttaaaa atatattctg
    58681 gagacaaatt gtatgtttca ttgatgtgat cgattagttc ttttttcgtt agcatctgct
    58741 tccatctctt tcttattgat tcgctttatt caaagatacc acgtaagaat agaaaaatga
    58801 atacaagtag ttgctaatca aatacttcga tatttttctt ttttatcaac tcttaaatgt
    58861 tcaatccgcc tgtgttggtc ttgctaccat ctactattaa aatgtttatt attgtttgcc
    58921 cacctatttt ttgattttag tggtatcatt tttcaagttc atgatgaaat tcttatcatc
    58981 agtaaatgct ccagaaaaat aaacaatgat tgaaaaatca gttgaaactt gaccaccgtt
    59041 tattttctcc gtattcttag aatagaaggg agtctgattg atgtattcac taatgaaaaa
    59101 aatcattacc gaaaaagata ttcgacgtca tgtttcttta gtagaacaat tgctgaatca
    59161 tacaaagata accgtaaatg aattagctga aatcataggg acaaccgaac gaacgatttt
    59221 ttctgacctt caatctattc gctctcagct gcccgaaggc tgggacatca tcagcgatca
    59281 agcaggaatc agtttgcaaa accagcaaaa tttattaaca aatgaccttt gggagatttt
    59341 tttcaaacaa tcagtcagtg tcgaactgct gaaaaatctg ctttttacaa aaaaagttgc
    59401 tgtccctgat tttctagccg accatgggct ttcttatggg acattgaaaa gacatgtgac
    59461 taaaatcaat cagcgtttag caagttacga cttgcagatc gatttgacga aatatacagc
    59521 ttgtattctt ggaaaagagc gtgcgattcg tactttttat catcgattgc tgattccttt
    59581 cactcataac aactattttt tcgaggatta ttcgatccat gaatcccatt attttcaatt
    59641 tttaagaaac ctcagtcaaa ctgaactagc ggttgaaaca gaagagattt tcggaacatg
    59701 ttggttcttt atcaatacga tccgtataaa agcgaattgc cgattagatt cttctatcca
    59761 taccaacagc acactctcct ccctgtatga ctctgcattg aaaaaattgt atttaacgga
    59821 aggtatttat cttaaagaca ctgagctttc ttttgcttct ttctgctttc tggaaagttg
    59881 gaattacaac aataactatg gacaagaaac cgctcgctgc ctgcatcatt ctccattttt
    59941 agaggtcctc gaaacgtttg tagaggaact agcttcagag ctgtctcttg atcaactaaa
    60001 aaaaacatct ctgactgata acttggctct cctcatatta aaatatcatg aatcgccgat
    60061 attgattgaa gcattggacc gtcaatacca tcgtttttta gaaacatatg atcagcaata
    60121 tgcacacttg tatcaacaga aagagaattt actgaacgat ctaaaaaaag aaatagaaat
    60181 caatgaacca aattactttc tccaactctt gtctctactg atccaacaaa cgattttttc
    60241 tatcaaaccg aatttgttca atgtctattt tattttccaa ggagaacctt catggaaggc
    60301 gttcttgcag caggaattaa aagattactt aggaaaacgt gtctgcttct tacctatcga
    60361 actaaccgat ctttccacta tttcttttga aaaaacagat atcttgattt ccaatttccc
    60421 tctagattct ctagatatcc ctattgtata catttcttct attccaacga aaaatgagct
    60481 caatcgtttg actgaactca ctttcaaaac ttttttatag taacggctag cgactgtatt
    60541 ctctagctgt tttttctttt ttgcgaaaca gtttgttcat gatccctttt tctatttcta
    60601 caactaagaa tagaagaatc acatttgctc caatcaataa ctgttgtgtc atcgttaaac
    60661 ttactgtacc gatcagctgc tggccgactg gtagaaaaat aaccatccct tgcaataaag
    60721 caaggatacc taatgaaaga aaaagcgctt tgttttgcaa aagaccttgg ttcaatgacg
    60781 gcttggacaa ctctctgcaa ttgatcatgt aaacagcttg agctaagacg atattttgca
    60841 gaagaatggt ttgctgtaag ctttgccctt caaactgcat agccaataaa taggcaggaa
    60901 ccatgatcag tagggagaca tagatgatcc gaaacaaatg ataaccagat aggattcctt
    60961 gctttggatc tcttggtggg cgcttcatga tttctggatc tggcgactcg aaccctagtg
    61021 cataagataa agtgattgtc gttaccatat tgacccataa aatctgtact ggggacaaag
    61081 gtagcgggcg attcagcata agtgcccaaa tcaagatcaa tccttgagct aatgcagttg
    61141 gcaagaagaa attgatggtt ttttgcaaat tatccaaaat ccgccggccc tcttttacgg
    61201 cttttgcaat cgtatggaaa ttatcatctc ctaaaaccat atcagctgcc tgtttgctta
    61261 cttcactacc tttgattccc atagcaattc ctacatcgga tcttttcaaa gcaggtgcat
    61321 catttacgcc gtctccggtc attccgacga tttctccatt tttctgcaaa gctttaacaa
    61381 ttcgcagttt atgttctggt gtcgttcgag caaagacatc gactattggc acttgctgaa
    61441 tcaactcttc atcagacata agatccaaat cgatcccttc taaaactttg gcagtatgtt
    61501 ttaagccaat ttgttccgca atcgcttttg ctgtgtcttt gtgatcccct gtaatcattt
    61561 tcactgagat ccctgcttgt tggcattcct ttacagcttt gactgcactt tcttttggcg
    61621 gatcgatcaa tccggcgatc cctgcaaaag ttaattgggt taaagtctca tgcgtgagtt
    61681 cctgtttcga atctactttt ttataagcaa atcctaaaac acgctgacct tttctcgcaa
    61741 attctgcagc tgtctgactc catgcttctt gttctgcaga agaaagagtt gatttagcaa
    61801 ataatacttc tggtgcacct ttgacgtaaa tcaccgctcc tttttcctcc gcatgcatcg
    61861 ttgccatata tttgtattca gaactgaacg gaatctttgc ttctattttt ttcaaaggta
    61921 gattggctgt ttctgtgtgt cgaatcagcg caacttctgt cggattgcca cttaaatttt
    61981 ctgttttctg ttcttctttt gtttttaatt cctgacagtt tttcataatg gacaacacgc
    62041 aggcttcttc ttttgcagct acatccacaa ctgtcatttc attttttgtc aatgttcctg
    62101 tcttatctga acagatcact gtcatagaac ctaatgtttc taccgaagga agtcctttga
    62161 tgatcgcctt ttcttttgcc atttcatgta ctccaacgga tagaatcatg gtcaaaacag
    62221 ctggcaatcc ttcgggtacc attgacacca ccaaagcaat cgtagccgaa agaaggatat
    62281 gccaatccat accatatcga aatgtcgtaa aaataactaa gaataggctc aaacaaagaa
    62341 tgccttgaaa aatttgtttg tttaatcggt tgattttttt aatcagaggc gtcgtttgtt
    62401 gttcgaccga ttgcaatgct tgattgattt taccgatttc tgtcgtgttt cctgtttcta
    62461 caacgacacc gactgcagaa ccagattgta caagcgttcc tgaaaaagcc atatttttct
    62521 ggtcgccgat cgattcttct tctgtcaaaa cggcatgact tttttcagat gcttctgatt
    62581 ctcccgtcaa aatcgattct tccactaata aatcatgtgc ttcgattaat cggatatctg
    62641 ctggaacgac atctccggca cgtaagacaa tgacatctcc ttttaccagt tctttcgcag
    62701 ggattttttc ttgcatcccg tttgatagta ctatcgcttc ttgtcccatc agctgcttca
    62761 aaccattcaa tgattcttcc gcttttcttt cttgccagta actaacgagt ccattgacga
    62821 tcactacgaa taaaataata cttccttcaa tatattcact agaagcaaat ttcaaaaaag
    62881 aggcaatgat caacacgatc atcagcagat cagtaaaatg ctttgctatt tttttccatc
    62941 gttttgtctg ctgccttgct tttatttcat tatatccttg atcagtcagg cgtttctgtc
    63001 gttcttcttg cgacaagcct tctgttgatg tttgatagtt tttcaataca tcttctaccg
    63061 tcaatctgta ccagttcatc ctatcccctc ctgtttaatt gatactgtta ttatacgcag
    63121 ggaagttttt tcaggcgtat cgattatttc aggggggtct gaaataattc gttttgcaaa
    63181 atgtatctgg gcctaactga tatttctatt gcttattctt ttatatacaa aatttaagaa
    63241 tacaaaccag ataaccaata aaaaaggaat aaagcgtgtc gtgctttcga ataacaagct
    63301 gaaagcaatt gaagtaaaaa acaccaacaa agcaagatct ttatatcttt gtgtagtgtt
    63361 ccgtttcttt tctttaataa attgaacatg tgcacacaag atcgaagacc acacaaatag
    63421 aaaacaactc gttgtcatgc ctgccaaaag ctgaaaaaac aatcgactat cagctacaaa
    63481 aaaatgtaga caaagaccgg caaaaaataa cattcctgta attaaaatac cgtgtaaagg
    63541 tacgcctttc ttagaaagcg cacaaaacca atggggcaaa cttttttctt ttgctgtttg
    63601 atataaaatc ctgctagtac tgtagactgc actatttgct gaggacaaag aagcaaccaa
    63661 aatcattaga ttgacaattg ctgctccgcc ttggcaatgg atcacttgca gcatagtcac
    63721 taaaggactt tcttttaaat ctaaacaatg ccatggaaca acaaataaaa tcgacaaaat
    63781 cgttcctata tagaaaaaga gaatacgcca aggcagacag ttgattgctt ttttcaaatt
    63841 cttttgcgga tcacttgtct cgccaacggt taagccgatc atttcaattc cagcaaaact
    63901 gaataaaacc ataggaaatg agactaccat tccagcaaag ccaaacggta gtaattcatc
    63961 gatctgtcca aaattttctt tgattccgat agactgggta tgaaaatctg agacactcaa
    64021 tcctataccc aataagataa aaaggaaaat ccccaagatc ttgatgattg agaaaaaaga
    64081 ttccaattca ccaaaccact tgactgcaac aaggttgact gccattaaga tcattagtgc
    64141 gctacctccg cttatcaaag ctggtacatg tacaaaccag aaatggatat acaatcctat
    64201 tgctgttaat tccgcaagtc cagctccaag ccagcaaagc caataagtcc aattgacaaa
    64261 aaatgcccac tttttgccta aataatagtc aacgaaatca ctgatagagt taaatgtact
    64321 atttgctaaa aataaatcac ccatggcccg catcaaacca taaatagcca aaccgcatat
    64381 caaataaacg agaataacag ctggccctgc tgatgcaatt gcctttgctg aacctaaaaa
    64441 taatcctgtt ccaattgcgc cacctaatgc gattaactgg atatttcgat tagttagagt
    64501 ccgtttcatt gccggttttt ccatcaagtt cacctttttc tttttcaagc agcatggcga
    64561 cttcctgttc ccaagaaggt ttctttattt tttcaataca gagtggtact acaaacaaaa
    64621 tgacactgat ggctgtgact gcaattagat tgatccatgt tccagctttc atcgtcagtc
    64681 cgacagaaaa tccgattgcc gcaaataaaa caataacgac agtccaaact ccaagattgc
    64741 tctttttctc tttttttcca attctaaatg gtcgattcaa ctttgcatgt gtataacgca
    64801 atctcaaaac tgcgatcccc atgatgacat agacgataca gtagataaca gtagttgaat
    64861 ttacgagcat caaaaatgct tgattgacgc caggaatcag caaataaaaa agtgcaaaga
    64921 ttgagataat gacagactga gtcaaaataa cggatttgga tacgtcaaat tgattcgtct
    64981 tccaaaaatt gaattttggt ggatacgcac cttttctagc actttctgtc actgtctttg
    65041 ctggtcctga ggcccaagca gataattgga ctgctacacc aatgaagaca agtaaactaa
    65101 acagattcac aataagatgt ggcaaaccaa gaatttggca ataaatcgaa atagattgcg
    65161 cgatattatt caattccatt tgtccttttg gtacgacatt tgctacaact aaagcattca
    65221 aaatattgac gaaaaatata aaaattaacg cggcaaatac tccgcgaata tacgttttaa
    65281 ctggattttc caatcgagtg atatacacag aggacatttc gataccagta aaaataaaca
    65341 tgatcggcgc aaaataaaca aatgaggatg cagtcgtagt atctggtatt aatttgtccc
    65401 atgaaaaact gcctaatgtt ccggttggta cgatcccaac tttgatccac gcagcaaacc
    65461 ctaataagag catcatcgtc aaaggcacgt acaatcctaa ccaaacaccg acttttccgc
    65521 cgatttttgc catatcaaat ttcaaattca aaagagtgat gatccaataa acgactaaaa
    65581 tgacaactaa tgtaaatttg ctgttcaaac caagcggcac attatcgata atattaccaa
    65641 acaacggagc gagtgctgaa gcgaccatca ccatcccagg aaacatttgg acccataaca
    65701 gccatgagac aacgaacccc catttttttc ctagagcatt cgatacccaa agttctggac
    65761 cacctttttg gggaaacatt ccagcaaatt caccagagat caatgagatt ggaaaagcgt
    65821 acaataatac tgcaaccagc atgtaaaaaa tcatcgtcca gctcgttgct gctacatctg
    65881 gaatatttcg tacacttgct acaagggcgc aggtcattcc gataaaggtc atggtcgtga
    65941 tttgctttga tgaaccagta tctttcatct ttattccttc tttcaatagc gacatatgtt
    66001 tttgttttct tttatttttg ttcaagaaca gaaatgacaa ggtttcatcg attcagtaca
    66061 aaacccagta tcaccgaaac atccattgaa cgatcttagt gaactgggtt tgatacatga
    66121 aacgaacgat ccaacttata gaacgtttta taaaaaagaa tatcttggcg aggaatatat
    66181 gaaaaacagg gggaaataca attgttttga tgaagcaaat aattgtctgt tctccatatg
    66241 tctcttgtta ttctttcaca caaaaatcag ttctattgga tgatttcgtt tagtttcttt
    66301 tccattgctt tggcgatttt ttttgtataa aaattgaaaa ttcgatcatc attcaaataa
    66361 ggagtcatga tagccgcacg tagcagagtg attttctgtt cttttttcca ttcttctttc
    66421 gttagaccca ttctttcaac aaaacgaatc ggagaatcac cgtaatcttc ttgtgtaaat
    66481 gtagtatgag aagtaataaa ccgttcacca taaacatcgc catccatata agaagaccga
    66541 tcgaacattt tttcatttaa ttcattgata gcgttcaaat ctgtacagcc ttgttctttg
    66601 aatacccaat tgaccatatt aaaatcagga tgattcaatg gatagacttc gattgttttt
    66661 cctttaacag taaaagtcag atgatcgaga aattctctga atctttgagc tgcctcgatt
    66721 gaagccccaa tcaattgacc atatccggtc acattcaatg gcaatacacg atgtgctgtc
    66781 cagactgcgg cggcggttgc tccagctttt gatccttcca aaatataagc tcctaacata
    66841 tctggagcct ttactgattt ttcaaacaca tatggcgcga aataagaaat gatatttctc
    66901 atatttttat gtttgattac gattcctcca gcagcataag ggacatagcc catcttatga
    66961 ggatctatcg tgacagaatc cgcttctgaa atagctctga acccttcata tacctctttg
    67021 tcaatcgtta cgcagtggtg gaaaacatga tgttcttcaa agaattcagc taaggaagca
    67081 tacggcacaa attctcctgc ttcgtttaga aacagtgaac gcgcatagcc gccatatgcg
    67141 gcatcaacat gtaaatagaa atagatccct tcatctttta acctttctct taattggacg
    67201 attttatcta cactgtctac ttgaccttct tctgtcgttc ctacgacagc aaccacacct
    67261 agaattggga ttttttgtcc agctaattca cgaattgttt tttccaaagc attaatatcc
    67321 attcgataat cctcttgaac tgggatagca accatctgat ctaagccgac tccgcaaata
    67381 tctaacgcct tcatccaaga atagtgtttt gtttgaggga ccagccattt ccctaatctt
    67441 tggatatttt ttccgctacg ggaagaagct gctttgacct catccatttc ttcgtccgta
    67501 aagctttcgg tcatttccaa aatttcttca acagataaat tcaaaagttc ccactcagac
    67561 atttttttca ctttttcagg cagcacttcc ttgacagcta aaggaatcga tttgatgcaa
    67621 cgggcgtacc ataatccttc aagattagca atagaaccat ctgctgcaat gtgtccccat
    67681 ccgtctgtca tattgaataa tgaagcaaaa tcctgtccta cttctgcttc catttgcgaa
    67741 gttgccatag aagattccaa tgcaacatta tttgggttcc acagcatcgc atagttgtaa
    67801 gctaataaag caggcatcaa tgtttcagca ttcatttgtc cccagtaacg gcctgctgaa
    67861 tgccatggga tcgatcctgc gcgtaatcgc tgactgactt catccaagac ctctatcata
    67921 tggtcgcggg tggctagata atctggagtg agcttatctc cttcgctaat tgcaggcata
    67981 tctgaaggaa tataattttc tctccatcca agatgttcat ctaccatctt gttcaacaac
    68041 attttataaa ctggtccatt ttcagctttg tcgcctataa agacggcctt gatatccata
    68101 tctttcattt ttcttccctc catattttgt tggtcaatgt cttgcagata catgtgttta
    68161 tctgtaacac ctcttcacta tagcgccaaa aatgagaaat ttttttttat aacatcaaat
    68221 ccacgtagat ttttacaaac aaaaataaat aaagtcggat ttttctgata attcaacgca
    68281 aaaaagcgaa caatttttca ataaatcagt cttctaaaat agataaccta aaaaaagcga
    68341 acaaacaaat a
//
LOCUS       DAZUKZ010000004        60875 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000004 DAZUKZ010000000
VERSION     DAZUKZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.004"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            199..2733
                     /gene="secA"
                     /locus_tag="ACG8EF_000240"
     CDS             199..2733
                     /gene="secA"
                     /locus_tag="ACG8EF_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFS4945600.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2859..3960
                     /gene="prfB"
                     /locus_tag="ACG8EF_000241"
     CDS             join(2859..2930,2932..3960)
                     /gene="prfB"
                     /locus_tag="ACG8EF_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFS4945601.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4065..4751
                     /gene="ftsE"
                     /locus_tag="ACG8EF_000242"
     CDS             4065..4751
                     /gene="ftsE"
                     /locus_tag="ACG8EF_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFS4945602.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4744..5628
                     /gene="ftsX"
                     /locus_tag="ACG8EF_000243"
     CDS             4744..5628
                     /gene="ftsX"
                     /locus_tag="ACG8EF_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFS4945603.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            5780..7228
                     /gene="cls"
                     /locus_tag="ACG8EF_000244"
     CDS             5780..7228
                     /gene="cls"
                     /locus_tag="ACG8EF_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS4945604.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7565..8422
                     /locus_tag="ACG8EF_000245"
     CDS             7565..8422
                     /locus_tag="ACG8EF_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFS4945605.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8537..9457
                     /gene="pstC"
                     /locus_tag="ACG8EF_000246"
     CDS             8537..9457
                     /gene="pstC"
                     /locus_tag="ACG8EF_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFS4945606.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9457..10341
                     /gene="pstA"
                     /locus_tag="ACG8EF_000247"
     CDS             9457..10341
                     /gene="pstA"
                     /locus_tag="ACG8EF_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFS4945607.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10356..11162
                     /gene="pstB"
                     /locus_tag="ACG8EF_000248"
     CDS             10356..11162
                     /gene="pstB"
                     /locus_tag="ACG8EF_000248"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS4945608.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11178..11936
                     /gene="pstB"
                     /locus_tag="ACG8EF_000249"
     CDS             11178..11936
                     /gene="pstB"
                     /locus_tag="ACG8EF_000249"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS4945609.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11949..12626
                     /gene="phoU"
                     /locus_tag="ACG8EF_000250"
     CDS             11949..12626
                     /gene="phoU"
                     /locus_tag="ACG8EF_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFS4945610.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            13203..13895
                     /locus_tag="ACG8EF_000251"
     CDS             13203..13895
                     /locus_tag="ACG8EF_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS4945611.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            13892..14659
                     /locus_tag="ACG8EF_000252"
     CDS             13892..14659
                     /locus_tag="ACG8EF_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS4945612.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14759..16327
                     /gene="liaX"
                     /locus_tag="ACG8EF_000253"
     CDS             14759..16327
                     /gene="liaX"
                     /locus_tag="ACG8EF_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFS4945613.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16624..16944
                     /locus_tag="ACG8EF_000254"
     CDS             16624..16944
                     /locus_tag="ACG8EF_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFS4945614.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16944..17294
                     /locus_tag="ACG8EF_000255"
     CDS             16944..17294
                     /locus_tag="ACG8EF_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS4945615.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17461..18399
                     /gene="hprK"
                     /locus_tag="ACG8EF_000256"
     CDS             17461..18399
                     /gene="hprK"
                     /locus_tag="ACG8EF_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFS4945616.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18414..19244
                     /gene="lgt"
                     /locus_tag="ACG8EF_000257"
     CDS             18414..19244
                     /gene="lgt"
                     /locus_tag="ACG8EF_000257"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS4945617.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19288..20313
                     /locus_tag="ACG8EF_000258"
     CDS             19288..20313
                     /locus_tag="ACG8EF_000258"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS4945618.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20328..21266
                     /gene="galU"
                     /locus_tag="ACG8EF_000259"
     CDS             20328..21266
                     /gene="galU"
                     /locus_tag="ACG8EF_000259"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS4945619.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21391..21831
                     /locus_tag="ACG8EF_000260"
     CDS             21391..21831
                     /locus_tag="ACG8EF_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFS4945620.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21834..22436
                     /locus_tag="ACG8EF_000261"
     CDS             21834..22436
                     /locus_tag="ACG8EF_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS4945621.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22495..23601)
                     /locus_tag="ACG8EF_000262"
     CDS             complement(22495..23601)
                     /locus_tag="ACG8EF_000262"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFS4945622.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23811..24812
                     /gene="ccpA"
                     /locus_tag="ACG8EF_000263"
     CDS             23811..24812
                     /gene="ccpA"
                     /locus_tag="ACG8EF_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFS4945623.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25149..27494)
                     /locus_tag="ACG8EF_000264"
     CDS             complement(25149..27494)
                     /locus_tag="ACG8EF_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFS4945624.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            27773..28675
                     /locus_tag="ACG8EF_000265"
     CDS             27773..28675
                     /locus_tag="ACG8EF_000265"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFS4945625.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            28888..30663
                     /locus_tag="ACG8EF_000266"
     CDS             28888..30663
                     /locus_tag="ACG8EF_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFS4945626.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(30729..32315)
                     /locus_tag="ACG8EF_000267"
     CDS             complement(30729..32315)
                     /locus_tag="ACG8EF_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFS4945627.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            32566..32790
                     /locus_tag="ACG8EF_000268"
     CDS             32566..32790
                     /locus_tag="ACG8EF_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFS4945628.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            32932..34683
                     /locus_tag="ACG8EF_000269"
     CDS             32932..34683
                     /locus_tag="ACG8EF_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFS4945629.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            34683..36467
                     /locus_tag="ACG8EF_000270"
     CDS             34683..36467
                     /locus_tag="ACG8EF_000270"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4945630.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            36793..37644
                     /gene="licT"
                     /locus_tag="ACG8EF_000271"
     CDS             36793..37644
                     /gene="licT"
                     /locus_tag="ACG8EF_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS4945631.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            37737..39623
                     /locus_tag="ACG8EF_000272"
                     /pseudo
     CDS             37737..39623
                     /locus_tag="ACG8EF_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
     gene            complement(39663..40565)
                     /locus_tag="ACG8EF_000273"
     CDS             complement(39663..40565)
                     /locus_tag="ACG8EF_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4945632.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            40796..42199
                     /locus_tag="ACG8EF_000274"
     CDS             40796..42199
                     /locus_tag="ACG8EF_000274"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS4945633.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            42199..43632
                     /locus_tag="ACG8EF_000275"
     CDS             42199..43632
                     /locus_tag="ACG8EF_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS4945634.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            43777..44703
                     /gene="trxB"
                     /locus_tag="ACG8EF_000276"
     CDS             43777..44703
                     /gene="trxB"
                     /locus_tag="ACG8EF_000276"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFS4945635.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            44917..46644
                     /locus_tag="ACG8EF_000277"
     CDS             44917..46644
                     /locus_tag="ACG8EF_000277"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFS4945636.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            46659..47009
                     /locus_tag="ACG8EF_000278"
     CDS             46659..47009
                     /locus_tag="ACG8EF_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS4945637.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(47101..47961)
                     /locus_tag="ACG8EF_000279"
     CDS             complement(47101..47961)
                     /locus_tag="ACG8EF_000279"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFS4945638.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            48053..48481
                     /locus_tag="ACG8EF_000280"
     CDS             48053..48481
                     /locus_tag="ACG8EF_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4945639.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            48643..48819
                     /gene="rpsU"
                     /locus_tag="ACG8EF_000281"
     CDS             48643..48819
                     /gene="rpsU"
                     /locus_tag="ACG8EF_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFS4945640.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            48846..49292
                     /locus_tag="ACG8EF_000282"
     CDS             48846..49292
                     /locus_tag="ACG8EF_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFS4945641.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(49446..50825)
                     /gene="brnQ"
                     /locus_tag="ACG8EF_000283"
     CDS             complement(49446..50825)
                     /gene="brnQ"
                     /locus_tag="ACG8EF_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS4945642.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            51531..52502
                     /locus_tag="ACG8EF_000284"
     CDS             51531..52502
                     /locus_tag="ACG8EF_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFS4945643.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            52526..54718
                     /locus_tag="ACG8EF_000285"
     CDS             52526..54718
                     /locus_tag="ACG8EF_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFS4945644.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            54735..55211
                     /gene="ybeY"
                     /locus_tag="ACG8EF_000286"
     CDS             54735..55211
                     /gene="ybeY"
                     /locus_tag="ACG8EF_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFS4945645.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            55195..55590
                     /locus_tag="ACG8EF_000287"
     CDS             55195..55590
                     /locus_tag="ACG8EF_000287"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4945646.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            55605..56504
                     /gene="era"
                     /locus_tag="ACG8EF_000288"
     CDS             55605..56504
                     /gene="era"
                     /locus_tag="ACG8EF_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFS4945647.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            56647..57459
                     /gene="recO"
                     /locus_tag="ACG8EF_000289"
     CDS             56647..57459
                     /gene="recO"
                     /locus_tag="ACG8EF_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFS4945648.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            57843..58760
                     /gene="glyQ"
                     /locus_tag="ACG8EF_000290"
     CDS             57843..58760
                     /gene="glyQ"
                     /locus_tag="ACG8EF_000290"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFS4945649.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            58762..60837
                     /gene="glyS"
                     /locus_tag="ACG8EF_000291"
     CDS             58762..60837
                     /gene="glyS"
                     /locus_tag="ACG8EF_000291"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFS4945650.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        19863 a        10760 c        13027 g        17225 t
ORIGIN      
        1 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
       61 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
      121 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
      181 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
      241 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
      301 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
      361 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
      421 cgtgtattgg ggttatatcc acaccacgta caattgatgg gtggaatcgt gcttcacgat
      481 ggaaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgaccgcaac gatgccagta
      541 tatctaaatg ctctgactgg cgaaggggta cacgtagtta ccgtcaatga atatttagca
      601 acccgtgact cgacggaaat gggcgaactt tataatttct taggattaac agtggggttg
      661 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgtga tatcacttat
      721 agtacgaata atgaattagg atttgactac ttgagagaca atatggtcgt ttaccgccat
      781 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc aatcttgatt
      841 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatccac agctttatat
      901 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatgta
      961 caatcaaaaa caatcagttt aaccgaagca gggatcgaaa aagcagaaga aacattcggt
     1021 ttggataact tatacgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     1081 agagccaact atatcatgct tttagatatc gattatgttg tccaagacgg aaaagtactg
     1141 attgttgacc agttcactgg tcggatcatg gatggacgcc gttattcaga tggacttcac
     1201 caagcaatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac catggcgacg
     1261 atcactttcc agaactattt ccgtatgtac aagaaacttg cgggaatgac tggtacagct
     1321 aaaacagaag aagaagaatt cagagaaatt tataacatcc aagtatacca gattccgaca
     1381 aatcgtccgg ttatccgtga tgatcgtcca gatttgcttt acccaacatt acaaagcaag
     1441 ttccacgctg tcgtgcaaga tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc
     1501 ggaactgttg cggttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     1561 cctcatgaag tgctgaatgc gaaaaatcac tttaaagaag cagagatcat catgaatgct
     1621 ggtcaaaaag gtgctgtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     1681 ttaggacttg gggtaagaga acttggtggt ctagcagtta tcgggacaga acgacatgaa
     1741 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     1801 tcacagttct acttgtcatt ggaagatgat ttgatgaaac gattcggttc agaacgaatc
     1861 aaatctttct tagatcggat gaagatcggt gaggaagatg ctgttatcca aagcaagatg
     1921 ctgacgaaac aagtagaatc tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt
     1981 aaaaacgtct tacaatacga tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag
     2041 cgacaagaag taatcatgga agaaaaaagc ttatccaagc cactaatgaa catggtcaaa
     2101 cggacaatca gtcgtgtagt agatgcgcat acacaattag agaaagaaaa ttggaatttg
     2161 gaaagtatca tcgattttgc cggcagtact cttgtccatg aagatagtat tactcttgca
     2221 gatatcgaag gaaaaacagc tcaggagatc aaagagtatc taaacgaacg agcacaagaa
     2281 gtatttaata ccaaagcggc tcaactaaat ggtccagagc aactgttgga atttgaaaaa
     2341 gtcgtgatct tgcgtgttgt ggactctaaa tggacagacc atattgatgc aatggatcaa
     2401 ttacgtcagt caatcggctt acgtgcttat ggacagaaca atccattagt cgaatatcaa
     2461 acagaaggct ataagatgtt tgaagatatg gtaggcgcca tcgagtatga agtcactcga
     2521 atctttatga aagctgagat ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca
     2581 gcttctcact cagaagacgg agatgccaaa gaaaatagta atactagcgc gaaaaaacag
     2641 ccaatccatg tgaacaaggt cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa
     2701 tataagaact gtcatggaaa aaatgcaaac taatttctga cagcaggatt ggtgagacaa
     2761 attctttttt taagaattcg tctcaccttc tttctgttat ttgctgattc tgttagacta
     2821 gatataatga aaaaagtata gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa
     2881 atctcctgac agaaatgcag gaaaaggtca caagcttcag gaggtctctt tgacttagac
     2941 cagctggaag aagacattgc ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat
     3001 gatagcgaag ctgctcaaaa agtgatcagt gaaaacaatg caaataaaga aacttatgat
     3061 caattccatt ctcttgctga agaactggaa gaaatcgaat tgatggtgga gatgttgcag
     3121 gaagaacctg atgcagccat gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa
     3181 aaaatgaaga catatgaatt atctatgttg ttagatggtc cttatgatcg aaacaacgcc
     3241 atcgttgaat tgcatccagg ggctggcgga acggagtccc aagattgggg cagtatgctt
     3301 ttacgtatgt atacacgctg ggcagagcag catgggtatc aagtagagac actagattat
     3361 caagcaggtg atgaagcagg aatcaagagt gtgaccctac tgatcaaagg acacaacgca
     3421 tttggttatc tgaaatcaga aaaaggtgtt catcgccttg tacgtatctc gccttttgac
     3481 tcagcgaaac gacgtcatac ttctttttgt tcggtagatg tgatgccaga gcttgatgaa
     3541 acgatcgata tagaaatcaa tccagatgat ttgaaaatcg atacgtaccg agcaagtggt
     3601 gcaggtggtc agcacatcaa taaaacagaa tctgctgtac ggatcactca cttgcctaca
     3661 ggaacagtag tagctagtca ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg
     3721 gggatgttaa aagctaagct ttatcaatta gaagtggaaa agaaagaaca agaagcagct
     3781 gcacttcgag gagaacaaaa agaaatcggc tggggttcac agatccgttc ttatgttttc
     3841 catccatatt ctatggtaaa agaccatcgg acaaacgtag agacaggaaa tgtccaagca
     3901 gttatggacg gtgaccttga tttatttatt gacggctatt taaaaatgca tctacaataa
     3961 gaacccattg ttaaattgaa ataaaattgt aagattttgc aagcatgttg aaacattgcc
     4021 atgctataat agctaaagac tgaaaaagat atggagagaa cgccatgatt gaaatgaagg
     4081 atgtaatgaa gaagtactca aatggtacga ccgctatccg caatctctca attgtcatcg
     4141 aacaaggaga gtttgtctat gtggttggtc cgtctggagc cggtaaatct acattcatca
     4201 aattgatgta tcgagaagaa aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc
     4261 taatagcaat caaaaaccgc gaagttcctt atctacgcag agaaatcggc gtggtgttcc
     4321 aagactataa attattacca agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag
     4381 ttatcggcag aaaacctcgg gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg
     4441 gtttgaaaca caaagttcga gtctttccta gcgaattatc cggtggggaa cagcaacgtg
     4501 tctctattgc tcgggcaata gtgaatacgc ctaaagtact tatcgcggat gaaccgacag
     4561 gaaatctgga tccagaaaac tcatgggaaa tcatgaaatt attagatcgg atcaatgccc
     4621 aaggaactac tgtcgtgatg gctacacata acagtacgat cgtaaatacg attcggcacc
     4681 gagtaattgc tatcgaaaac ggccgcatta ttcgagatca agcggaaggg gaatatggat
     4741 acgatgatta gaaccttttt ccgccattta ttagaaagta tcaagagttt gaaacgtaac
     4801 ggctggatga cagttgcttc agtcagtgcg gtaacgatca cattgacttt agtcggtgta
     4861 ttcatggcag ttatcatgaa cgcaactaaa ttagctcaag atatagaagg aaacgttgat
     4921 gtctctgtat tcgtagatat cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa
     4981 ttgaatgact tagatcatgt taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa
     5041 aaaatccaaa acgaaatggg tgattcctgg aaattgtttg aaggtgacaa taacccgtta
     5101 tatgatgtgt atgtagtaag tgctactgat ccttcttata caaaacaagt agcagaagaa
     5161 gcagcagatt tgaagaatgt ttctcgttct gattacggtg gagcttcctc agatcggatt
     5221 ttccaaatcg ctggtgcagt tcggacatgg ggattagttg cagcggcatt gctgctgttc
     5281 gtagcgatgt ttctcatttc gaacacgatt cggatcacga ttttgtctag acagcgtgaa
     5341 attcaaatca tgcgtttagt cggtgcaaaa aatggatata ttcgctggcc gtttttcctt
     5401 gaaggtggct ggatcggtct gatcggtgcg attcttccga ttttgatcat cacatttggt
     5461 tatgcatggt ttttccaatt gatcaatcca tcattattac gttcccatta ttcattgatt
     5521 catccacaaa atatcgtttg gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt
     5581 ggttcacttg gctcgatcat ttctatgaga cgtttcttga aattctaata ccacaatata
     5641 aataaaatga gaaagttgga cagtttcgtt ccagctttct ttttttattg gctaggagaa
     5701 tagaaggaag cggctttttt ccctttactt taaaaaacaa cttgggtatt cttaagttgg
     5761 tagaagaaga ggtgaagccg tgaaaattat cgttgacaat ttttttacga tccttttgat
     5821 tatgaatatt ttgctttcct ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac
     5881 atgggcatgg gtgctcgttt tgatgttcat tccagttgta ggatttattt tatatatttt
     5941 tttaggaaga gggatttcca aagagaaaat ctttgatttg aagatccaag ggaaaatcgg
     6001 taagaacctg gaaattgaag aagacaaaca ggcattgatg cgagggcttt atcctcatcc
     6061 gcctactgga caagtagatg tgaaacaatt gatctatatg ctcacggtat ttgaaagtac
     6121 gctctatacg actaaaaatg aaatcacttt atttacagat ggtcgtgaaa aattcgatgc
     6181 gctgattgaa gatatcaagc aggcagaaga ccatatccac ttccagtatt atatttatcg
     6241 cagtgatgca ttaggtgaag aagttcgaga tgcattgaca gaggcggcaa gacgaggggt
     6301 aaaagtcaga gtccttcttg atgcatgggg ttccacacaa gtttcttcca gcttttttca
     6361 gaatttgaaa aaagcaggcg gagaagtcgc atttttcttt cctctatttg ttccgtatat
     6421 taatcctcgg atcaattatc gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg
     6481 atatacaggt gggttcaatg ttggaaatga atatctagga ttggtaaaga aatttggtta
     6541 ttggcgagac aatcatttgc gtatttacgg agaagcggtg tataccttgc aaaaccgttt
     6601 tttgatggac tggaattctc agcatacaaa agaagtaggc tattctccca aattttttcc
     6661 gtccattcac tctactggtg atatagctgt tcaaatcgtg acaagcggac cagataccga
     6721 acatgaacaa atcaaaatga cttatttgaa aatgatttcg ttagccaaac gggagatatt
     6781 gatccaaacg ccttattaca taccggatgg atcgattcat gaagcactga agttggctct
     6841 tctttcaggc gtaaaagttc atatccaaat acctaacaaa ccagatcatc ttctcgttta
     6901 ctgggcaacg tattctttcg ctgcagagct aatcgagtat ggagcaagga tcgaaacata
     6961 tgagaatgga tttattcatg caaagacgat gattatcgat ggtggcattg tttcagttgg
     7021 ctcagcaaat atcgatgttc gttcatttcg cttggacttt gaggtcaata cactggttta
     7081 tgatgaacga atggcttcac gtgtaagaaa agcttttttt gaagactcga aaatttctac
     7141 gcatctcaca aaagaaatgt atgaaaaccg aggaataatc atcaagatga aagaaggatt
     7201 agcaagattg atttcgccgc tcctttaaaa cataaaaagg acatgagaac catcaacaga
     7261 tggagatctc gtgtcctttt ctaacggggc catttctgag agtacgtcag aaatggagaa
     7321 agcgtctcgc atgagcgaaa ttaactttgg ggtatctctt gttttttggg gaaagcaaga
     7381 aataagctaa tatatgctat taaaagagac acatttcaaa gccagtatag catagaaact
     7441 tttaatgaga tagttttttt tacaattatg agaatttatt ttgttattta atgattttga
     7501 ttcgttttca tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt
     7561 tctaatgaaa aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg
     7621 ctgttcaaag tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca
     7681 gcctttagta gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa
     7741 tgtacaaggg ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat
     7801 cggaaactct gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga
     7861 tcatcgagta gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga
     7921 catcagtatg gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt
     7981 cggtggaaaa gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag
     8041 cacgtttgaa aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc
     8101 aagcggaatg gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt
     8161 ttcttatgtt tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga
     8221 aaatgtgaca acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca
     8281 tccaaaaaaa ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa
     8341 catcgttgga gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca
     8401 aggaaatatc gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac
     8461 atgttattaa aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat
     8521 taagggggag tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc
     8581 aaaaatggaa cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt
     8641 tgtattatct attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa
     8701 gatcaatgta tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa
     8761 tgggaaacca atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt
     8821 atctgctatc gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc
     8881 tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc
     8941 tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg
     9001 cgggactggg tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac
     9061 tgtcacaacg atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc
     9121 attagcgctt ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt
     9181 gcccgggatt ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc
     9241 tatccaaatg gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc
     9301 tacattgacg tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa
     9361 taatgtgtta tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt
     9421 gatccgtatg attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa
     9481 attgctacag gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta
     9541 ctactttata tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct
     9601 tccaaagcct accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg
     9661 cttttgatca cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca
     9721 gaatatgctg gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc
     9781 tcgttgcctt ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag
     9841 tatggtttct cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg
     9901 actagaacag ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg
     9961 gccttaggtt tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca
    10021 gggattttga ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg
    10081 atctatacgg ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt
    10141 gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag
    10201 atcaatacag aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg
    10261 ttgattattg ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac
    10321 cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa
    10381 caaatgtcat caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg
    10441 tctggtatgg cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga
    10501 tcactgcact gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc
    10561 gaatgaatga tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag
    10621 atattaatgc gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc
    10681 aacgtcctaa tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc
    10741 acggcgaaaa aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg
    10801 ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc
    10861 aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag
    10921 acgagccggc aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa
    10981 acttgaaaga agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta
    11041 tcagcgacta tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca
    11101 agatatttac cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt
    11161 aagacggagg cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt
    11221 atggaaagaa agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg
    11281 cgatgatcgg tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga
    11341 atgacctgat tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct
    11401 atggtccaaa aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc
    11461 caaacccatt tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg
    11521 aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt
    11581 gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc
    11641 agcgtgtatg tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac
    11701 caacaagtgc gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa
    11761 gagagcggta tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag
    11821 ataagacggc attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat
    11881 tcatgaatcc agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag
    11941 gaaacggaat gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct
    12001 acgaaatggg gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac
    12061 acgataaaat cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa
    12121 cacgcttgga gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt
    12181 tgcgtatgat cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg
    12241 cggtgtctgt tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg
    12301 aaaaagagat ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg
    12361 cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca
    12421 atgactattt caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga
    12481 cagtcatcag tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact
    12541 atgtgacaaa catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa
    12601 atacgaacca caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa
    12661 agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc
    12721 aaacaaaaaa tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc
    12781 ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca
    12841 tcatttcata gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta
    12901 tttgtgtgct tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca
    12961 tcgagataga aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga
    13021 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
    13081 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
    13141 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
    13201 ccatgttgct tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
    13261 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
    13321 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
    13381 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
    13441 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
    13501 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
    13561 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
    13621 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
    13681 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
    13741 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
    13801 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
    13861 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
    13921 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
    13981 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
    14041 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
    14101 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
    14161 cctactattg cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact
    14221 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
    14281 gttgcttctg attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg
    14341 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
    14401 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
    14461 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
    14521 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc
    14581 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
    14641 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
    14701 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
    14761 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
    14821 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc
    14881 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
    14941 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga
    15001 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
    15061 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
    15121 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
    15181 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
    15241 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
    15301 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
    15361 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
    15421 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
    15481 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
    15541 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
    15601 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
    15661 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
    15721 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
    15781 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
    15841 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
    15901 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
    15961 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
    16021 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
    16081 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
    16141 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
    16201 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
    16261 tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
    16321 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
    16381 atagcttccc ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc
    16441 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
    16501 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
    16561 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
    16621 gacatgatga aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta
    16681 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
    16741 ctgagcttag ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc
    16801 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
    16861 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16921 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
    16981 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    17041 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
    17101 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    17161 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
    17221 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata
    17281 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
    17341 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
    17401 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
    17461 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
    17521 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    17581 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    17641 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    17701 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg
    17761 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    17821 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    17881 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    17941 aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga
    18001 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    18061 catctgattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    18121 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    18181 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    18241 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    18301 gcgatgaatt tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    18361 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    18421 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    18481 tcatcgtatc aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag
    18541 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    18601 gcgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    18661 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    18721 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    18781 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    18841 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    18901 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    18961 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    19021 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    19081 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    19141 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    19201 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    19261 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    19321 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    19381 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    19441 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    19501 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    19561 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    19621 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    19681 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact
    19741 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    19801 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    19861 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    19921 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    19981 ggtgccaacc cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc
    20041 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    20101 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg
    20161 atggaactag cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    20221 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa
    20281 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa
    20341 aagcagttat tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg
    20401 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    20461 tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    20521 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    20581 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    20641 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg
    20701 tcgttatgct aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg
    20761 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    20821 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    20881 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    20941 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    21001 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    21061 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    21121 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt
    21181 taggtaaaac attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    21241 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    21301 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    21361 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    21421 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    21481 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    21541 acaaaagacg tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa
    21601 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    21661 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    21721 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    21781 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    21841 aaaaaggtgg atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt
    21901 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    21961 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    22021 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    22081 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    22141 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    22201 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    22261 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    22321 aagcaaaacc agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga
    22381 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    22441 acaaatttat agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt
    22501 ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac
    22561 atatacacag tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag
    22621 agagaagcac atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc
    22681 gtggacggtt gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata
    22741 gctgttgatc acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac
    22801 ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc
    22861 ggttggtttt tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac
    22921 gccaagatcg aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg
    22981 agggcttgca gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc
    23041 ttgttttttc aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt
    23101 cactgcttta aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt
    23161 gtctttttcc tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt
    23221 gtaagaatcc gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc
    23281 gttcttttca atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat
    23341 gatctcccaa gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt
    23401 ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat
    23461 aaaaagagct agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg
    23521 gccagggtcg ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc
    23581 attgattttt acgttattca tttggcaacc tactttctaa attaattttg tattcaataa
    23641 ttgtaccact gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc
    23701 atgaaaacag agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa
    23761 tgtaagacgt attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc
    23821 aaacaatcac tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac
    23881 gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta
    23941 tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa
    24001 caacagttgg tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg
    24061 gtatcgatga tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca
    24121 acgaccaaaa agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga
    24181 tcttcatggg tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc
    24241 ctgttgtatt agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt
    24301 atgtatccgc aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg
    24361 cttttgtcag tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata
    24421 aagaagcatt aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat
    24481 acaacttcaa agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg
    24541 tctatgtttc agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg
    24601 tgaaagtacc ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag
    24661 ctcgtcctcg tttaacaagt atcacacaac cattatacga tttaggggcg gtatctatgc
    24721 gcctattgac gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt
    24781 atggaatcga agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt
    24841 gcctgccgtt taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga
    24901 tgaatattgt gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg
    24961 gttatgacaa aattttgtta taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt
    25021 gaaattacat ttatcgtata taaaacaatc cgtgtataga tgctgcattt tgttcaccac
    25081 catcaaaaaa ataagggact gtgacaatac tattgtcaca atcccttatt actgtgtatt
    25141 ctattaaatc aatcatcttt ctcatctgat tctttagttg cttgattcgc tcttgcataa
    25201 gctgaagcgg tgttccagta atctgtatag ttttcatcgg taccgccaac gccaaattta
    25261 aaggcgcttt tcttcggtcc atttttcgcc catagcgatt caactgtttt tcctggtgtt
    25321 tggatggatt ttttattgac agtaactttt ccaggtgttt gaccagtaaa tccagatact
    25381 tttttcttca tcacatcaga tgataattcg aatttctcat cagtgccaaa aatgtcaggt
    25441 tttgcttggt aagcttgatt gatcagattg gcaagataat tagccgaacg tatccttgct
    25501 ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc tactgatggt aatagacgga
    25561 gtagagacaa ttagccatga atctcggtat tcattggtag aaccagtttt tccgacccaa
    25621 tctgcttttc ctagatttcc gtttaagctt gagatggcat ctttgaatgg tgtagtgatt
    25681 tttgcattga tcacactacg catcatgtca ttcatgatcg atgccgttgc ttccgaatag
    25741 atccggacag agttgctttc gtgtttataa attacatttc ctgcgttatc tgtgatggaa
    25801 tcgatgatat agccttcttc atagacgccg ccgtttgcta aagcctgaaa tccgttggtc
    25861 tgttgaagcg tcgtgacatc tactgtacct aaaggagcag attcaacgcc ccaattgtca
    25921 tttgccgggt agttcatctt tgccaagtag tgctcgtaag catattgttt cgatccaccg
    25981 gaattcaaca catcttgata taagtgataa gccgggatat tgttggacca ttctagtgac
    26041 tcacggacgg tttggaaggt gttcgatcct tcgttagtag cattgacgat tttctcgcca
    26101 gcattttctc cttcttgcca agttgttgca taatctgata cacgggattc tgatccgata
    26161 agccccatgt cgattgcagg tccataaaca agaactggtt tgattgatga gccagcttga
    26221 cgttctgtat cgaaggcatg gttattttga ttctgactgt aattccgtcc gccaacgaat
    26281 ccgtagatgc ggccagttct gttatccatc aatacatttc cggtttctac ttgactgcca
    26341 gcaccatcat ctaacatgta tccataattt tcaacgccat tttgcaatgc agcatagaca
    26401 tctttatcga ttgtcgaatg gattgtatat cctttatttt ggatcgtttg ctgtgccttt
    26461 tgataataag cgtcactgac cgatgaatca gacatatcag cgttgtcttt ttcagccaat
    26521 tgttgtgcaa tgattcttgt tgcttcattc atcacagtat aatacaagaa ttctctgtca
    26581 gtctgttctg cgatttgttg tggtagaaag tcttttgtaa ggtcataggc ttttgcttct
    26641 tcgtattgtt ctttcgtaat ttggccttca cgatacatac tgaataaaac aaaatctttt
    26701 ctggcaaggc cggcagaaag atcttctttt agcgcgcctg tgttcgtgta tggactatac
    26761 gtgatcgggc tttggggtaa tccagcgata taagcagctt gcggcagcgt cacatctttt
    26821 gcatctacac caaagattcc ttttgcagct tcctggactc cagcaatgtt ttgtccctta
    26881 ttattacgac caaatggtga gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag
    26941 tatttttcta cttgtgcaga aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca
    27001 tctgtaagga tctgctgttt gactaattgc tgtgtcaaag ttgatccacc agaagagcca
    27061 ataccagtag cttcagatac taatgctcgt aaaacagctt taggaacgta accttggtgt
    27121 ttatcgaagt attcatcctc tgtgctgata attgcagttt tcaataaagg tgaaatctta
    27181 tcagatgaaa tagtcgtgcg catcagatct gtctggattt ttgatatctc tgtattatct
    27241 gcataaacaa tctttgacgt ttctgtaatg tttcctagtt ctgtctgcag tgctttttta
    27301 gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc cagcgcctat tccaatacct
    27361 agagctgcga acatcaaaag aagaacagta ataaaaacag tcaatgattg aaaaactcta
    27421 aaaataatct taggaacaaa ccatttttct ttttttcttc tattctgttt ttttcgagta
    27481 tttttgcgtg gcaaaagggt cacctcaact aatggtttta tttcgctaat tatagcaaat
    27541 aatagaacaa aaaaacagac aaggctgttt ttggcaggaa tttctttttt aaaagagtta
    27601 aaacgtgaga aaatgatctt tattataaaa gagttcttaa gcttttcttt agagaaaatt
    27661 tcagttacta tggagaacag ggcaaagaaa aatcaatcat tttctttgta tatcatctta
    27721 ccaatatata ggaattgtta gtaacaattg cctttcaagg aggaatccat ccatgttatt
    27781 aggctcacac gtcagcatga gcggaaagaa aatgctgtta ggagcagcag aagaagcagc
    27841 cagttatgga tcttcgactt ttatgatcta tacaggcgcc cctcaaaaca ctagaagaaa
    27901 agccatcgaa gagatgaata ttgatgccgg aaaagcgtac atggcagaaa aaggtctaac
    27961 aaatatcgtc gttcatgccc cttatatcat taatttgggg aatacgatca aaccagaaat
    28021 gtttcctttt gccgtccaat ttctgagaga agagattgtt cgtgcggaag ctttaggcgc
    28081 tactcaaatt acacttcacc caggtgctca tgtaggggca ggaacagaag ccggaattgc
    28141 tagaattatt gaaggcttga atgaagtttt gacaaaagaa cagacggcac aaatcgcttt
    28201 ggagacaatg gcaggtaaag gtacggaaat cggtcgaaca tttgaggaat tagcgcaaat
    28261 catcgatgga gtaaaattga atgaaaaatt atccgttact tttgacacct gtcatacgaa
    28321 tgatgccggt tataatatta aagaagactt ttcaggtgta atggaagagt ttgataagat
    28381 catcgggtta gatcggctta aagtactcca tatcaatgat tctaaaaatc cgcaaggttc
    28441 gcataaagac cggcacgcca atattggttt tggaacaatc ggattcgaca cgctgaataa
    28501 gatcgtccat gatccaatgt ttgacaatat ttcgaaaatc ttggaaactc cctatgtagg
    28561 caaagataaa aagaaagctt acgcaccata tggaaaagaa atcgccatgt tcaaagcgca
    28621 aacattcgat ccagaagtgt ttcctgagtt attagcagaa gaagtgatac tctaagattt
    28681 tctatctgac agcagcaaaa aaggaccagg cacattgcct tgtccttttt tgtttaagaa
    28741 acaaaggagt actttttgca gtctttttca tcatgcatct tctcccccct ctttttaaat
    28801 ttagacaaac aaggtatact ggtagaaaag taaaaaaacg aaaagtttca caagcaagtg
    28861 gcaaaacgtg attggaagga cgaaaagatg ctgaacactt taaaaccctt gatcaaccaa
    28921 ctaaaaaaga aacatacata ctttattctt ggcatagccc tattatttac aattattcgt
    28981 ttgtatttat atagtaaagc aatctatgga acagacatgt cggcaaccta tgatgaccag
    29041 ctgctgattc gctatgctca aaatattttg gacggtaaat ggctagggga gtatacgact
    29101 acaacacttt ccaaaggaat ctcctacagc ttatttttag tacttgcaca taaactgcat
    29161 ctttcttatg cagttttgtt cggcatgttc agtatttttg ccagtatatt ggttgcactc
    29221 gcgtttaaac cgattgtgaa gaacaagtat cttcttttaa gtatctattt ttattttttg
    29281 ttttctccga ttagttttac acatgaatat tccactcgaa catatcgtaa tgccctcgtg
    29341 attccggctg ttgagatcat tatcgcttgt ttattagcta tttattaccg caaatacggt
    29401 tctagcagaa aattgctgcc atggttttta gggctttctg tcgcttttcc tttcttttgg
    29461 ttcattcgcg aagattctat ctggcttctt ccttttactg tagtagcttt gggtatcagt
    29521 ttcgtccaga tcttgctagg gcaagacttt tctttcaaag aagtaaagaa gttaaaaaaa
    29581 cgactctttc ttctctcgaa acgggaatgg ctgaaacttt cattgttttt acttcctata
    29641 gttggctttt ccatcgtcta tcaatggatc gaatataaaa atgagacaac atacggtatt
    29701 gcaaccatca atgaccgttc cagtggagaa tttggacgtt tgatgaaaca actgatccga
    29761 atagatgatg aaacgaactt gaataaagaa aacagtgaga tatgggtctc acatgctgca
    29821 ctaaataaag cattagccgc atctcctaca ttccatactg tttctacccg tattgacgaa
    29881 ttatatacga ctcacgcgtg gactcaaggt ggaaaagtaa aagaattacc cggagatatc
    29941 atcttttggg cattgcgaga tgtggtaagc gaatctggtt attaccaaaa caatgcaaaa
    30001 gaaaccaatg atttttggaa aaaagtcaac caagaattag aagaagccta tcacaaggga
    30061 acaatcaaaa agaaaaaaga gttctatctg atgggatcag gagatggaaa gacgatgagt
    30121 gatctgccaa tcgtattcga tttccttcaa tcaggatacg cttatgcatg gcgatataaa
    30181 gatttccatc aaggaggaaa ttatagtttc ggaacgcaag aacaagtcaa acaggctcaa
    30241 gaattattaa accttccgct aatggagaat tggatcgata gtaatgaacc gagaacacct
    30301 aatttccaat taacaaaatc agcaaaagtc gcgaatgtca tcataaaaat ttaccaagtt
    30361 ctggctattc ccgtgctgat tgcttcaata gcagggatat tacttatgat actaggaacc
    30421 gtatttgcaa aagctgatcg tgcctattat agttcctctt tgattgtggt caccggatta
    30481 atactgacac aattggtttt tcttttcggt gtctcatggt tttgttctta tgcgccagaa
    30541 aaaaaggact attttttaag tgtctacact ggtgcaggcg tgccattaac gcaattcatc
    30601 gtagtaacaa gcatgctagt tttcagtaaa ttaaatctga aaaaagcttt atcttctaaa
    30661 taaaaagacc cttggtagct agatttttaa cctaactatc aagggtcttt gtgtggaaaa
    30721 atcagttttt attcgatttc ttctaccgtt tcctgcaaaa gctgttcata ttttttgttt
    30781 cctttatata aatcaccaat cgtccatgcc aacaggagaa ggacaagaac aatgattaca
    30841 taagcgatgt gatcagccaa tgctagttga gcggaagtgg cttgctcgcc gaagaaactt
    30901 tcgatctgtc ctggtaatcc tcgtaagttc aagtaagtta gactaatcac agagaaccag
    30961 ccaagaagtt ttacgatgaa tgaattttta aatcggctac ccatctccgc ttggctgttg
    31021 gtcatcatca gtaaaggaat catcgaaaac ggcaacgcaa aagccaagaa cacctgggat
    31081 tcattcatta agttattcaa agcggcatgt tcttcgatcg ttccttttgt acttgtaaac
    31141 aaaacacaaa tcaatacagg aatcacagaa agcaatcgtg tgaccagtct tcttagccaa
    31201 atcgggatac gcatatggat gaatccttcc atgatgactt gaccggtcaa cgtaccagta
    31261 atagtagagt tctggccaga agcaagaagt gctacagcaa ataaagtgga caagatacca
    31321 gattttgcta cagcaatcaa aataccattg ctcatagctg atggatcaga taaagcttca
    31381 tacagaccaa aaaaggaagg atcttggaca gaaccggttt tgaagacagc gacacccatg
    31441 atcaatagca gtgcattgac tagaaaagcc attgacaact ggatattaga atcccaagta
    31501 gaaaaacgta cagcttgtgc tacatcttct tcgttttggt gatcgatctt ccgagtttgt
    31561 gaaactgctg aatgaagata taagttatgc ggcatgactg ttgcaccaat aatccctaaa
    31621 gctccagtca gcggagtttc gccgccgatg accggtgagt ctgagaatgt atgactgtta
    31681 gggataaagc cagcaaataa actgtgccaa tctgggttag acaagccaac ttgatagaca
    31741 aaaacgaata aaatgacgaa aattaagcag acaaccagtg cttcgatctt acgaaacccg
    31801 acttttgtca gtagtaagag aacgaaaaca tctaatacag taataaagac ggcaatcacc
    31861 aatggaatat caaaaagtaa gtagagggcg attgctgcac caatgacttc tgcaatatct
    31921 gttgccataa tggctaattc agtcaagatc cataaaatga cacctaatgt cttacttgtg
    31981 cgtgctcgga ttgcctgtgc caaatccatt tggctcacga ttcctaattt tgctgccata
    32041 tactgtaaaa gcatagcaat cagactagac atcaaaataa ctgacatcaa taaatattgg
    32101 aaattttgtc ctccggtgat cgaagtagac cagttacccg ggtccatata cccaactgct
    32161 actaacgcac caggtccaga gtaagcaaga agtgttttaa aaaaactttt cccctttggt
    32221 acatcaactg ttccatttac ttcagctaaa gataaaccat ttgtatgttc aataagtcga
    32281 tgcttatgaa attgtttatt ggattgatta ttcaagaaaa aaccaccttt cctatttatc
    32341 tacgattaca ttctacacct caaaaagaaa aaattaaaga aaaagattcg gtacgcctaa
    32401 ttttttttca tggcgcgtct tgctagatga cgaaagaaat tttgttctcc tctaaaaaaa
    32461 ccactatttt ttgcatgaaa ctaaagaata cgcctttctt ttttcaataa actttagtaa
    32521 aataagtcat tgagagcaat tcggaaatag aaaggagaga tttatatgtt atcaacaggt
    32581 attgtcgtac ttattgctat tattgctttg ctaatcggcg cagtaggtgg ctttttccta
    32641 gcgcgtaaat atatgaaaga ctatttagaa aaaaatcctc ccgttaatga ggacatgttg
    32701 cgatcaatga tgatgtcaat ggggcaaaaa ccatctgaga agaaaattcg ccagatgatg
    32761 cagcaaatga agaaccaagg gaaaaaatag gttctgaccc aacgactttt tacgggctga
    32821 ttttttcagt ccgtttttta tttcgtaatt tgttagacta ttcagattat tcagcaaaaa
    32881 agacaagcac agacaatcct aggctcttga taaagacaaa ggaggagtaa aatgtcgatt
    32941 tttagaaaat taggctggtt ttttcgtcag gaaaagaaac attatttaat tggggttttc
    33001 tcgctattcg cagtggcctt ggtacagtta gttcctccaa aagtgattgg gatcatcatt
    33061 gatgagatcg ccgataaaaa tttatcaata aagatcattt tcgggtggat cttcctattg
    33121 attatcgctg cttttttaca atatcttttt cgttatatct ggcggacgaa tatttgggga
    33181 agtgcagctc gattggagaa agaacttcgg cagcagttat tcaaacattt tacagaaatg
    33241 gacaatctct tttaccaaaa atatcggact ggggatttga tggctcacgc aacgaatgat
    33301 ttgaatgcga tccagaatgt agcaggagct ggtatcttga cctttgcaga ttcagtgatt
    33361 acgggcggaa caacgatcat tgcgatgatt ttgtttattg attggcgctt aacactgatt
    33421 gcgctgattc ctctgccttt gctggccgtc acttcacgag tgctggggtc aaaactccat
    33481 gatgcttttc gtgattcgca agaagctttt tctagtatca atgataagac acaagaaagt
    33541 atcactggga tcaaagtcat taaaacattt ggacaagaaa aagaagatct agcggatttt
    33601 gaagaaaaga ttgaagatgc aattattaaa aacaaacgag tcaatttttt agactcctta
    33661 tttgatccat tcattacctt gattatcggt ctgtcttatg ttgcaacaat tattttaggt
    33721 ggaaagtatg tcttggaagg cacgatctcg atcgggcaga tggtctcatt tattagttat
    33781 atcggtatgc ttgtttggcc gatgtttgcg attggtcgat tgttcaacgt attggaaaga
    33841 ggaaatgctt cttatgatcg cgtcaatgaa cttttgcatg aaaagacgca tatcgtggaa
    33901 caactgggtg cagtgcagac cccggcaaaa ggcgagctga atgtagctat ttcctctttt
    33961 aattatccga aagaagaatc accagcatta gaacaaatca agtttgcgat ccaagaagga
    34021 gaaacattag gtattgttgg gaaaactggt gcgggaaaaa cgacgatcat caaattactg
    34081 atgcgtgaat acgatgatta tcaagggaaa atccaatttg gcggacgttc gatcaaagaa
    34141 tattcattga atgctttatt aggatcaatc ggctatgtgc ctcaggatca cttcttgttt
    34201 tcgatgacca ttcgagataa tatccgtttt tctaatccaa aactttcaga agaagaagta
    34261 gagcatgcgg cttctttagc atttatcaac aatgaaatca aggaattacc aaaaggttat
    34321 gataccttag ttggagaacg tggagtctct ttatctggcg gacagaaaca aaggatttcg
    34381 attgctcgag cattgatcaa aaatcctgaa ttattaatat tagatgatgc actctctgca
    34441 gtagatgcca aaacagaaga agctatcttg cataatttaa aagaaataag aaaagagaaa
    34501 acaactatca ttgcagctca ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt
    34561 gtcgatcaag ggaaaatcat tgaacgagga acccatcatg agctgcttgc actaggcggc
    34621 tggtatgaac aaatgtggga gaaacagcaa ttagaagcga agatagaagg aggcgaggcc
    34681 tgatggatga aaattatcaa tcggaatgga caaaatcgat tccacttaaa gaacagaaaa
    34741 agatcatcaa acgactgatg aagttcgcca aaccttttag aagaacgttc atcgttgcta
    34801 tcttatttgc gtttgctttg tctgtgatca atgtcctatt gccaaggatc atccaaactt
    34861 ttatggatga tcatctggct aaacagtcgg ctacgacaca agttattttg ttttttgctg
    34921 gtatgtactt atttggagtc atagtaaaaa gtatcatttg gtttttccaa tggtatttgt
    34981 attccatggc ctcattgaag acctatcagt atattagggt aaaattattt gaaaagctgc
    35041 atacactagg tatgcgttat tttgatcaga caccggcagg atcaaccgtt tctcgagtga
    35101 cgaatgacac agaaacgcta tttgaatttt ggtacgtctt tttgatggta ttgactggga
    35161 tctttgcagt cgtttcttca ttccttgcca tgtttcaaat caatggagaa attgccttgt
    35221 ggactttggt gtttttgccg attttaggaa tcgtcatctg gtattaccag aaattcagtt
    35281 ctcgtattta ccgaaatatg cgggaaaaat taagtcagct gaacacgaaa ttgaatgaat
    35341 cgatttccgg catgcgtatt atccagcagt ttcgccaaga agctcgttta gctaaagaat
    35401 ttgaacaagt gaacaatgag tatttagaca cgcgttatgc gatgatcaaa acgaattcga
    35461 ttctgttaag tccgatcatc aattttctat atgcattggc gattgcgttg gtgttgactt
    35521 tgtttgggat cgatgcgttg cattctcccg tagaagttgg actgatctat gcatttacga
    35581 cctatgtcca gggatttttt aatccaatgt ctcaaatgat ggactttttg agcatcttca
    35641 cagatgggat ggttgctggt agtcgtatct tgaagattat ggatacggaa gaatatgcac
    35701 cacaacaaaa ccctggagca gatcaaatga ttacttcagg taagattgag tttcgcaatg
    35761 taagcttttc ttatgatgga gagcacaatg tattaaacaa tatctctttt gttgctaatc
    35821 cgggagagac cgttgcatta gtgggacaca ccggaagcgg aaaaagctcg atcatcaacg
    35881 tattgatgcg gttttacgaa ttttatgaag gtcagatatt gattgatgat catgacattc
    35941 gagaatatcc aatggaagag ttacgtaaga aaatggggct ggtcttgcaa gatgctttct
    36001 tgttttatgg ggatatcgca ggaaatattc ggatgatgaa tccagctatc acagacgaac
    36061 agatacgcca tgcggctgag tttgttcaag caaacaaatt catcgatgaa ttaccaaaag
    36121 gatatcactc aaaagtgatc gaacatggtg ccagctattc tagtggacaa cgacagctga
    36181 tcacttttgc gcgaacaatc gtgactgatc ctaaaattct agtattggat gaagcaacag
    36241 caaatatcga tactgaaaca gaaggattga tccaagaagg attggcaaaa atgcgtcaag
    36301 gacggacaac gattgcgatt gcccatcggc tgtcaacgat tcgagatgca gaattgattt
    36361 tagttttaga caaaggtcat atagtagaac gcgggaatca tgaaacactt ctagcgcaaa
    36421 atggtttgta tgcagatatg tacaaactgc aaagcagtgg tatataaaag atgttgtgac
    36481 agtagaattt tgacctaagc actagagtga aaataaaaca tagcttctca tatttttcat
    36541 ctgtgagagt tagtgtcgta gtcaccagat ttaaaaaaag attcgacagc tagtcagaag
    36601 tgcctaatag ccactaagaa agcgttgaca aaagcatttg ctttcgataa tataaagaaa
    36661 acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt ctcccatggt
    36721 ttcatggacg gctttttggg tttttctttt tgttttggat acagccctat acatcatgga
    36781 aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat aaccagggat
    36841 gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa gaaagtgggt
    36901 gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga cgggctttca
    36961 caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat ttcttgtgaa
    37021 atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt gtatattgca
    37081 ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt tgtaccaaat
    37141 gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg gaaaaaaaca
    37201 gtgaagaaaa tctcggataa ataccacatc aagttatcag aagatgaagc cggatttatc
    37261 gcattgcatt tagtcaatgc acaaggcatt caaagccagc aagacaatac ctacctgttt
    37321 actataacaa tacaggatat tctaaatatc gtccgctatt actataagat tacatttgat
    37381 gaagattcag tccatttttt tcgattcgtc acacacttac gtttttttct gacacgcgtc
    37441 ctagatggag aaaaatcaga agtgggagaa gtttctgagg aattgtttca ggttattcaa
    37501 aaaaaatata aggatacgtt tggttgcgtt gagaaaatag cagacttttt agccgagacg
    37561 ttcaactacc agatgtccac ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta
    37621 gtgcatgaga atcgttgtaa ataaaaaaag gaggataaat taacaagaac gtatgaaata
    37681 agggtagtga cattcagttg gctaaacctt ccactaataa attggaggga atagaaatgg
    37741 gaaaatacca agaattagcg agaaaaatcg tagaaaatgt cggcgggaag gaaaatatca
    37801 atggtttgac acattgtatc acaaggcttc gtttcaaatt aaaaaacgaa gaaaaagcaa
    37861 atgatgagat cttgaaaaat atggacggta ttgtaacggt aatgcgagcg ggagggcagt
    37921 atcaagttgt cattgggaat catgtgccag tcgtgtttga agaggttata aaagcaggaa
    37981 accttacttt tgatgaggct gtttcaacag aaaaaatgag accgtttgat atgcttattg
    38041 atggaatatc gggctgtttc cagccattcc ttgctatcct agctgctggc gggatgattc
    38101 gtggactgac agcattcctt gtcttcttag gcgcgtttga ccgcggttct ggcacatttg
    38161 ttatgtttga taatatcggt gattctgtat tccaatttat gcctgttatt atcggtttaa
    38221 cagctgcccg taaatttaaa gtaaatgaat tcgtagggat gttgcttggc gcagcattaa
    38281 tgaatcctag cttgtcgctg gaagctttga gtggtgcagc agaagcacca ctaacgacca
    38341 tattttctgg cactattttt gaagcaccta tttatcaaac agtattcggc attccctgga
    38401 ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt tattgtgttg ttagctagcc
    38461 aaattcagaa accaattaaa aagttagtac ctgagatgat cgcgaatttc ttcgttccat
    38521 tcttcaccgt tcttattaca atgcctttag gttttttact cgttggacca gtattcacat
    38581 tcgcaactga tattttaatg gctggatttg aaacactttt agcactttcg ccagttattt
    38641 atggagcaat cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc cattgggcaa
    38701 tcgtaccaat gggattgatg caattttctg tgaatggttg gcaaaatatt atgacacctg
    38761 ttgcagtagt gtcttttgga caaactgcag cacttaccgc actctacttt aagcttagaa
    38821 atccaaaaga caaagcaatt gcaataccag ccattgtttc tggtattgtg gggattacag
    38881 agccagctat ttatgggttt acattaccac gtaaaaaaat atttattttt acctgtgtcg
    38941 gtggtgcgtt agggggcgcg tatagtgggt taatgaattt aacttcttgg aatcaaggtg
    39001 gactaggcat ctttacaatt cccaactata ttagaccaga tggtgattta acagatgtga
    39061 ttaatgtatt gattgggatc gccattgcga tggtggtatc ctttacgctg acttttttct
    39121 tttggaagga tgaagcaggt gaaacagatg atatacagaa aaaatcaggt aaagaaattg
    39181 taaaaacacc gatacaagga caaatcgctc cactaaatgc agcaaaggat gcagcttttg
    39241 cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa gtacgtgccc
    39301 cttttgacgg aacagtcatg accctgttcc cgacgaaaca ggcaattgga atggtttctg
    39361 aaacagggct ggaattgctg attcatgtcg gattggatac tgtgcagttg gaagggaaat
    39421 attttgaaag cttagtgcag caaggataaa gagtgaaaca aggcgatctg ttgctacgct
    39481 tcgacaaaaa gaaaatcgaa aaggaaggat acagtttaga aacacctgtg atcgtcacca
    39541 attacacgga ttatttggat attttggaaa accaagggga gacggtcggg ccagacgatg
    39601 cacttatcac cgcactgacc tgaacggtta ggtgaagaat ggactaggct taactattct
    39661 ttttattcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc tgagctgata
    39721 gggcgtttat cgaatttttg accgacagaa attagatgga ctaaattctg ttgataattt
    39781 tctgcaaaaa cacaactata taacacgtct agaagataac aaattgatgt attgatcgta
    39841 aaattaccaa tttttgaata cagtcgctcg cgtgtcgtaa gtcgcaaagc ggctgtactc
    39901 attgcagata gcgttgaatc accgatactg gtcaaagcta aaattggaac atgattttgt
    39961 ttcaatattt ctgcggtacg aaggatcaca ttgttttcac ccgtataaga aatcagaatc
    40021 ccgcacatat cactcgtgca attgtacgct tcatagaatg attcaccaaa agtggtgaca
    40081 atcgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga aatcaaagta
    40141 ttcgagttac tggcgaaaat cacgattttt tttgcttgaa gcattagttg tttggctttt
    40201 tgcagttggt catgatgaat gagagaaagt gtatcatgaa tcgtcatctg ttctaaagaa
    40261 gccattttat ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag attcgcatct
    40321 acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt acgaaaactg
    40381 acccagccat caaaacccat tttttttgct acacggatga gtgtagaggg gtggacataa
    40441 catcgctcag caatcgcttg gatggtcatt tcgccaagtg ctggagaatc gcttaagaga
    40501 aactggatca cttcattttc tgaaggagaa aaggagattt ctttgatttt ttcacgtaat
    40561 aacatgtaga taccttcttc ttattttttt acgtaaatta taactttttt gaaaatgatt
    40621 tgtaaacgga tttatactat ataacttaaa atagacattt tttgacaaga tatttacaaa
    40681 aatccactag aaaacagtaa attttgtaaa tgttactgga aagaaacaga tttctattgt
    40741 ttgttttgtt tacaaaaaag ataatgaaag tgtaaaaaag taaaggagtg agaacatggc
    40801 aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt tacacccccg agttgatgga
    40861 aggatttatc aaaagatatg aggaattacc gatacgagaa atctggctcg tagatgtaga
    40921 agaaggaaaa gaaaagcttg agatcgttag tcagatgtct cagcgtatgt gggatgcttc
    40981 tccgtatgaa gtcaagatct atgcaactct ggatcgggaa gaagcattga aagatgcgga
    41041 ttttgttacg actcagtttc gtgtcggtca attagatgca cgaatcaaag acgagcggat
    41101 ccctagttat tatgggatga ttgggcaaga aacaaatggt gctggcggga tattcaaagc
    41161 ttttcgtacc gttccagtcg ttttggcaat cgttgaggac atgaagcgtt tatgtccaaa
    41221 tgcttggctg atcaatttcg cgaacccaag tgggatggtg acagaagcag ttatcagata
    41281 tggcaaatgg gataaagtga ttggattatg caatgtgcca gtgatggcga tgatgacaga
    41341 accagcaatg cttggcaaga aaaaagaaga gctgatctac aagtttgccg gtctaaatca
    41401 tttccattgg cacaaagtag cagataataa aggaaatgac cgcacggcag aattgattga
    41461 tcgtttatac gatgaagaga atggattacc gaaaaatatc tttgatatcc cgtttttcaa
    41521 agaacaatta aaacaaatga acatgatccc ttgtggctac caccgctatt actatcgtga
    41581 agaagaaatg ctggaacatg ctttagaaga atatcaaacg attggtacaa gagcgcagca
    41641 agtgaaagaa acagaagcag agctatttga attatacaaa gatccagaat tagaccataa
    41701 acctgaacaa ttgcaaaaac gaggtggcgc ttattattca gacgctgcct gtgaaacgat
    41761 tgcttcaatc tatggaaata aagaaacaca gatcgtggta tctacaaaga atgatggagc
    41821 tgttcctgac ctgccggcag attgtgtagt agaagtcact gcctatgttg gcgcacaagg
    41881 agcgagaaat gtagcgtttg gttctcttcc tgctgctgaa agaggctggt tgcaagtaat
    41941 gaagaatatg gaattgctga cgatcgaagc agctgtgact ggtgattatg ggacagcttt
    42001 acaggcgttt acgatcaatc cacttatacg atcaggaaag acagcaaaac ggatcatgga
    42061 tgagttgttt attgcacacc agcagtattt accgcaattc aaggagacaa tcagccgatt
    42121 aaaagaagaa ggaatcgaag ttcaagatga actggcaaga gaattatcag gtaatacaca
    42181 tataatggag gaagtataat gggattcaga aaagattttt tatggggcgg tgctacagca
    42241 gccaatcaat gtgagggcgg ttatgatgaa ggtggacgcg gactagcaaa cgtagatgtt
    42301 gtgccaatcg gaaaagaccg ttttcctgtt atcaccggaa agaaaaaaat gtttgaattt
    42361 gatgaggatc atttttatcc agcaaaaatt gcaatcgata tgtatcatcg ttataaagaa
    42421 gatattgctt tgtttgcaga aatggggttc aagacttatc gaatgtccat cgcctggagt
    42481 cgtatttttc caatggggga tgagaaagag ccgaatgaag ctggcttgaa attttatgag
    42541 gatgccttta aagaatgcca aaagcacgga attgagccac ttgtgacgat tacgcatttt
    42601 gattgtccaa tgcatttgat caaagaatat ggtgcgtggc gttccagaga actcgttggt
    42661 ttttatgaaa atctgtgtcg agtcatcttc aatcgctata aaggacttgt caaatattgg
    42721 ctgacattca atgagatcaa tatgatcctt catgcaccat tcatgggcgc agggctgtat
    42781 tttgaaaaag gagaaaatga agaacaaatc aaatatcaag cggcacatca tgagttggtt
    42841 gcaagtgcca tcgcaacgaa gattgctcat gaagtagatc cggagaacaa agtaggctgt
    42901 atgctggcag caggagcata ttatccatac acttgcaatc ctgaagatgt ttgggcttct
    42961 agaaaatcag atcgagaaaa ttatttcttc attgatgtcc agtcacgagg agaatatccc
    43021 gcttatgcat tgaaagaaat ggagcgaaat ggcattcaga tcgaaatgga accaggggat
    43081 gccgcattgc tgaaagaaca tactgttgat tttatctctt tttcttatta tgcttcccga
    43141 gtttcgacta ccgacaacga actcttggaa cagactgcag gaaatatctt tgcttctgtc
    43201 aaaaatccat atctgcaagc aagtgaatgg gggtggcaaa ttgatccatt aggcttgaga
    43261 ataacgatga atgatattta tgatcgttat caaaaaccat tgtttattgt agaaaacggc
    43321 ttaggtgcag tcgatacgcc agatgaaaat ggttatgtgg aagatgatta ccgaatcgat
    43381 tacctggcag cgcatatcca ggcgatgaag gatgcagtag aactagacgg agtcgatcta
    43441 ttaggctata cgacttgggg atgtattgat ttagtatcag ctggtaccgg tgagatgaag
    43501 aaaaggtacg gattcattta tgtcgatcgt gataaccaag gaaatggcac attagcccgt
    43561 tcaaagaaaa aatcattcaa ctggtataaa aaagtaattg attcaaatgg agaaaatctt
    43621 tcgaacgatt gatcaatgat ttataaaagt ctattgtttt tcagcggatt tttcgagtat
    43681 aataaagcta ttcgagaaac gcaagcagaa caatagactt tttttcgcat tggctgtaaa
    43741 gtatgcttac ttgattaagt aaaggagaat ctacatatgt atgatgtaat cgttattggc
    43801 gcaggtcctg ctgggatgac tgctgcttta tatgcttcac gttcaaactt atccgtattg
    43861 atgatcgaac aaggtgcccc tggcggccaa atgaacaata cggcagaaat cgaaaattat
    43921 cctggattcg attcgatcat gggccccgat ctagcttata agatgtatga aagtgtctca
    43981 agatttggga cagaaaatgc ttatggaatc gttcaagata tcaaggatca cggcagccat
    44041 aaagaagtca tctgtgaaga taaaagttac gaaggaaaaa cagtcattat cgcaacagga
    44101 tgtatccacc gtaaattagg cgtacctggc gaggaagaat ttgctggacg aggcgtttct
    44161 tactgtgcag tctgtgatgg tgcgtttttc cgtaacaaac gtctagtagt catcgggggt
    44221 ggggactcag cagtagaaga agccatctat ttgacacaat ttgcttctga agtagtgatc
    44281 gtccaccgta gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc atttgcaaat
    44341 gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa cgaaatggta
    44401 gtgacaggtg tgaaagcacg taatgtcaaa accggcaatg aatttgattt agcagcagac
    44461 ggagtattca tctatgttgg actggatcca ttaactgcac cttttagaga agcaggtttg
    44521 acgaacgaag ctggctggat tccaacagac aacgaaatga aaacgaatat gccaggcgtt
    44581 tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa tcactacagc tgttggtgac
    44641 ggcggtattg ctggacaaca agtgtataaa tacttggagt ctttagaaga aacaacagca
    44701 taagtttaaa aagagtagaa gaacgggttc aattgttctt ttactctttt ttttcgcgtg
    44761 aaatttactg aatctgattt atgcgaacac gtccaacacc ttttgataaa agaatccttt
    44821 cttgatttaa gcgttttttg gtcctcatac tttcatatct aataagtgaa tgctatactt
    44881 attaagaacc atatttcaga aagaggtgtt attttcatgt cttgggaaca agtgtatgaa
    44941 caatgggtac atgaagaaaa tttagaagaa aacttaaaaa aacaattaaa tgatctgggt
    45001 gaagatcctg aaaaacttga agatgctttt tatgcaccgt tggaattcgg tactgcaggt
    45061 atgcggggaa tcttaggacc tgggatcaat cgaatgaaca tctatacggt aagacaagca
    45121 acagaaggac tagcacgttt catggataca caaggtgaag aaacaaaacg ccgaggtgtc
    45181 gctatcgctt atgactcacg ccatcagtca ccagaatttg caatggaagc agccaaaaca
    45241 ctagcacatc acaatatccc gtcttatgta ttcgaaagtc tgcgtccaac accagagctt
    45301 tcttttgcag ttcgtcattt gaagacgttt gcgggaatca tggtaaccgc atctcacaac
    45361 ccagcggctt acaatggata taaagtctac ggagaagatg gcggacaaat gcctcctgca
    45421 gacgcagatg cattgacgaa atatgtccgt gaagtcagca atccgctaaa agtcgaagta
    45481 ttatctgacg aagaagctaa acatagcggg ttgatcacga tcatcggaga agaagtggac
    45541 gctgcttatc tagaagaaat caaagcagtg acgattgatc gtgaattggt tgaaacaatg
    45601 gggaaagatt tgaaacttgt gtacacgcca cttcatggaa cagggaaaat gttgggcgaa
    45661 cgtgcgttga aacaagctgg attcgaacaa ttcgtactag ttcccgaaca agctgtggca
    45721 gatccagatt tcactacggt caaatcgcca aatccagaag aacattcagc ttttgaatat
    45781 gctatacgac ttggagaaaa agaaggagct gacttgctga ttgcaacaga tcctgatgca
    45841 gaccgtttag gcgcagcggt tcgtttgcca gatggttctt atgaagtatt gactgggaac
    45901 caaatcggag cattgtttgc tcaatatatt ttggaagcac atcaatcaag aggcacttta
    45961 ccagaaaacg cagcggtatt gaaatcaatc gtttccagcg aattgcctac agcaattgcg
    46021 gaacactacg gtgctaccat gttcaatgtg ttgacagggt ttaaattcat tgctgaaaaa
    46081 atccaacaat ttgaagaaga ccattcacat acttttatgt ttggattcga agaaagttat
    46141 ggttatttag ttaaaccttt tgttcgcgat aaagacgcga tccaagcatt gcttttatta
    46201 gcagaagtag cagcttacta taaaaaacaa ggcaaaacgt tgtatgacgg cttgcaaacg
    46261 atttttgaaa cctatggcta tttcgcagaa aaaacgattt ctgtgacaat gagcggacaa
    46321 gaaggttctg cgaaaatctc tgctttgatg gaaaaattcc gtaaagaagc accgacagaa
    46381 tttgccggag caaaagttat tcaaacagaa gactttaaag aattgacgag aacagaccgt
    46441 gaaggaaacg tagaaaaact aacaacaccg ccatccgacg tattgaagta tacattggaa
    46501 gaaaatggct ggatcgctat ccgcccttca ggaactgagc cgaaaatcaa gttttatatt
    46561 ggtgtaaaag gaagctctac agaagacgca gccgaaaaag tagcctctta tgaagcgatt
    46621 atcaaaaaga tgacagaaga ataaaaaact tgagactgat ggaaaaagtg gtaaacttta
    46681 gaagtcagtc ttttttcaag ttggaggtga atggattgcc agacacgcaa aaatcaaaag
    46741 tagagaaagt cagtcaatta ttcaaaatgt tgagtgatcc cactcgtctt aaaattctgc
    46801 tatatctgaa agacggggaa caaaatgtga cagcaatcac ccaagcagtg gaaatggagc
    46861 agtctgctgt atcgcatcaa ctacgtttat taagagaaaa tcatgtcgtc aagtctcatc
    46921 gcgagggaaa agcgattttg tatagtcttg atgattctca tgtactggat attttgaacc
    46981 agacactcaa acatgtggaa catcaataac gataggtgaa aatagataaa cactggagtg
    47041 tgacaacgaa gaaatttcgt tgtcacactc cagtgttttt taagaaaaag acaaaaatga
    47101 ttaatcgctt ttttctgttg tttcagttcc ataataacta tgatcttcta ggtctaaagc
    47161 attcaaaata agcgaagccg tttcttcaat agataaagat gccacattga tcactacaca
    47221 tcctagtttt tcataaagat cattcgcaaa atcgagttca gctctaattt tttcgatatc
    47281 ggaataagca gtatcagggt ttaatccgta agagcgcatc cgttctttac ggatattatt
    47341 taaaatatct gggttattcg ttagtcccac gattttttta ggatcgattt cccacagctg
    47401 tttgggaata tgtgcttggg gaatcaaagg taaattagca acttttaaat ttttatttgc
    47461 taaaaacaag ctcaaaggcg ttttggaagt tcgggatacg cctagcaaaa cgacatctgc
    47521 ttctaaaaaa ccacgaggat ctttcccatc atcgtatttt acagcaaatt ccattgcttt
    47581 gatccgtttg aaatagttct cattcaataa atggagggca ccaggttgcc gaattggctt
    47641 gataccagtt aaacgttcaa tttctgatat cggtggcgtc atcacatcga aactaaataa
    47701 ttgatgttct tcacaaaaag aacgtgttat ctcgatcagt tcttgattga ttaatgtatg
    47761 caggacaagt gctttttctt ctaatgcatc ttgcaatgcc tgccttaatg ttttttcatc
    47821 tgtcacgaac gttctgcgga ataggttgaa ctcaacggta ggatactgag ccatagtagc
    47881 ttgcgctagt tttgtcgcag tttctccagc agagtcggag atgacaaaaa aagtaacgat
    47941 ttctgtagaa tcggaagaca tggataaaac ctcacttgca ttttttttat gtttattata
    48001 ccatactcta gaatgcgtac ttcgtaatca ttttgatttg gaggaataag tcatgaaaaa
    48061 acagacattg atcgaacaat cattagaaac attgaaaaat aacggtttta aatatactaa
    48121 aaaaagagaa gcattgttaa cttatttagt gaagcgtaac cgctacgtat ctgctaaaga
    48181 agtttttgac tatatgaatc aggaattcaa aggggtaagt tatgatacga tttaccggaa
    48241 tctccatgat tttgcagaga tcgatttact ggaagaaacc gatttaaatg gtgaaatgaa
    48301 gtttcgtttt cattgctgtc agggggaaat cggccatcac catcatttta tctgtacagt
    48361 gtgcggaaca acgaaagaaa tccatatgtg tccaatgaat tatttcgaag aacagctagg
    48421 tggatgcacg atcgaagggc atagattcga gattttcgga agatgtgaaa attgtcaata
    48481 aggatttaaa taaaataaca tttgataatt taaaaaaaaa gcaagatttc cttgacggtc
    48541 aaaacagcat tagatataat atttacagga cagtcttgtt ttttgagata tatttcaata
    48601 acaattacgg ttacttttta agctcggagg gagggaatta acatgtcaaa aacagtcgtt
    48661 cgcaaaaacg aatcacttga cgatgctctt cgtcgcttca aacgttccgt gtcaaaagcg
    48721 ggtactttac aagaatctcg taaacgtgaa ttctacgaaa aaccaagtgt aagacgcaag
    48781 aaaaaatctg aagcagctag aaaacgcaaa aaattctagt tctgtgatga attggagttg
    48841 gtagtgtgtc actactaagt agattaaacg atgacatgaa agcagcgatg aaagcgaaag
    48901 ataaggaatc tttacaagtc atccgaatga tcaagtcatc tatacaaaac gagcagatta
    48961 aagaaggtca cgacttgaca gaagaagaag aactaacggt gttgtctcgc gaaatgaaac
    49021 agcgacgtga ttctttgcat gagtttgaag aagcaggacg tgacgattta gctgaaaaag
    49081 taaaaagtga aattgtaata gtcgaaaaat atatgccgga acaactttcc gacgaagaaa
    49141 ttcgacaact tgttcaagaa gcaatcactc aaactggtgc ttcatcaatg aaagaatttg
    49201 gcaaagttat gggtgcaatt atgcctaaag tcaaaggaaa agcagatgga aaccaagtga
    49261 atgcgattgt gaaagaacta ttacaatctt aattttatca tttaaactgc ggacagtgat
    49321 tgtccgcagt tttttgtgtt cgtaaaaaaa gaactaaaaa acagctagaa gaaagaataa
    49381 gattctttct tctagctgtt tttttcctgt ttaatatctc ttatgttaaa ttgtgtttga
    49441 tttggttatt cttctggtag ttcatttgct tcttgtttat accctgatag gcgataggcg
    49501 taataaaaga gtaagctgcc agcgtaacca agtaacgttg ggaccatcca gccaagtcct
    49561 aatgcagcaa aaggaacgtg atgatgataa aatcctagta agcttgctac gacagtttgc
    49621 tgactcacta atgcaggcga atttgccaac atatccaata cggctggaac ggcagaaaag
    49681 aacatcgtta cttgatagac aaatggactc ttttggaaga attttgctgt taaggaaagc
    49741 aagattaacg caagtgacaa tggatacaaa agcatcaata ctggtaaaga ccattggatg
    49801 atattatcca atccggcatt cgcaacgaca aaagagacaa aagtcgttaa ccgcagccat
    49861 gtcatataac tgactttagg gaacagttta tggaaatctt gtgcaaaaga aacaacgagt
    49921 cccatagcgg tagtgaagac acctaatgtg accattaccc ctaagaagct agcaccgaag
    49981 ttacctaaat aaaaattagt gatctgagaa agtgcagttc cgccattttc tgataaaggc
    50041 atcttattca aactcatcgc accgagaagg accagaccaa aataaatcat gacttctaac
    50101 gcaacgctca ggctaccagc ttttgctgtt aattctgtga cttttttatc tttaaaacca
    50161 agatcacgaa ctgcatggac aaaagtgaca cttaaagcaa gtaaagctac tgcatcaacc
    50221 gtgttgtatc cttctaaaac gccatttgtc aaagcgttcg tctgataaga atctgcagca
    50281 gattggttta gattccccat gggttggaat aaagaaacga caaaaatgat agctaataat
    50341 gcaagaaaca aggagtttaa atactttccg acatatttga ccaaacgtgt tggatgtaca
    50401 gtaaggaaat aagcaagtaa aaagaaaaga gcagagaata aaaacatacc taatgaatca
    50461 tatttttcag gcaaaaaggg tttagcagcc atttcaaaag cagttgctgc tgtccgcggt
    50521 gtaccaaaga atggtccgat cgttaaatga acaaggatca agaacaaggc agcataccat
    50581 tttccgacag gtttagctaa gtcatacaga ccatcacttt ttgtcactac tacagctaag
    50641 atagctaata atgggaaaag agcgcctgag atggcaaatc ctacaccagc gctgatccag
    50701 tttgatccag ccatttgacc taagtggaca ggaaagatca gattacctga cccgaagaac
    50761 atcccgaaga taagtgaact gaccatcaat aattgcttcc atgaataagt ttttgtttct
    50821 tccataaatt tttcctccaa taattatcat tttgagtaac aaaataaacg ttgcagaaac
    50881 tagaaatgcc actaatatca gattccatga aaaccaccac cttaaaatat aaaaaaaagc
    50941 ccttttccac ttgggaaaag ggctctagat tctagaacgg taccaccttt tttcgcttaa
    51001 aaaagcttct tcattcacgg acgcttttcg ataaagttat ccgctagcct gataatggag
    51061 gcagccacaa tgatctacgt tttcgacatt gactcggaaa tgatgttcgt ctattcgatt
    51121 tattgtcatt tctcacttac atgactcact gggaataaat cgctgaatag cctactgttt
    51181 tcctcgacgc tttgctgtat atttatttgt tgacgttata atacacctgt tttttttgaa
    51241 atgcaagaat atttcaaata ttttgttttc tttttttgaa acaagaaaaa tacccagaga
    51301 atacttgcta agttagacgg aatacttgtc ctctatctta tgcatagctt acatgatcag
    51361 ctttttttat tcaaacgaga cctttagaga atggaagcag ctatttttta acgtttcttg
    51421 gtacttatgg ttaagcaact cttttagaac cttttcttat ttgctatata gtttcttctg
    51481 agtatggtaa aataggaaag aatgaatcga aaaaaagggg atgtctattt ttgacggaag
    51541 agtctagttc tttagagatt agattgacgg ctgctgatga tgtcagcatg cttttaggtg
    51601 cacacgacaa gcatataaaa ctgattgaag aaacaacaga aacaaccatc catacacgtg
    51661 gagaaatcat tcagatcatt ggcgaacaat cccagatttc cttggctgcc tcagtcattc
    51721 ggacattgca ggaattgatc aaacgaggaa tccatgtaag tacaccagat gtcgtgacgg
    51781 cactgaaaat ggggcaaaaa ggcacattag attattttgt tgaaatgtat gaggaagaga
    51841 tcgtcaaaga ccgtaatgga aaaccaatac gggtaaaaaa cagcggccaa aagaaatatg
    51901 tcgaatcggt agccaaacat gatatcgtat ttggcgtagg accagccggg acaggaaaaa
    51961 cttttcttgc agtagtatta gcaatcgctg ccttgaaaaa aggtgaagtg caaaaaatca
    52021 ttttgacgcg gcctgctgtg gaagcagggg aaaatctagg ctttcttccc ggggatctaa
    52081 aagaaaaagt agatccttat ctgcgtcctg tttatgatgc tttataccaa atttttggat
    52141 tagatcatac gaaccgattg atggaacgag gcgtgatcga aattgctcct ttagcttaca
    52201 tgcgggggcg gacattggat gatgcattcg ttattttgga tgaagcacag aacacgacag
    52261 ttgctcagat gaaaatgttc ctgacacgtc ttggttatca atcaaaaatg attgtcaatg
    52321 gagatacgag tcagattgat ctgcctcgag ggacaacaag tggtcttgtt catgcagaac
    52381 gaacattaaa gcaaatcaag aaaatcgatt ttgttaattt tgaagcaagt gacgttgttc
    52441 ggcaccctgt agttgccgaa atcatcaaag cttatgaaaa ggcagatctc catcaagaat
    52501 aagctttgct taggaggata agcacatgtt gaataaaaca ctgcagaaac tacaagaaaa
    52561 acttggaaag gcagcattgc ctttcctgct gttcttgttt tttatcatta tctgcggcgt
    52621 tacatttagt agtgtcaaac aaaaggccaa tgatttcaga gaaggccaag tagcagaaga
    52681 aagtatccgt gcgaacaaaa cgatcgagaa cacggaagaa accgaacaaa aaagaaaact
    52741 ggcagcagaa gcagtcactc cagaatacac ttaccaaaaa gatcttgcag atgaccagaa
    52801 caatcggatc aagcaactat ttgaattgat cgataaaacg aatgatgcca tcaacaaatc
    52861 ttatgacgaa aagattgata aggcaaagga agatgaaaat atcccaaagc ctactacaga
    52921 agagcggatc gccgcgttga agaaaaattt tgaaaatgtg aatgcagaaa atgttgcgtt
    52981 ttatcaaaaa ctaccgaaca atttttattc gacgatattt gaaatgactg aaactgagat
    53041 ccatactgtg agggatgaga gtctgaagct cattgacgaa cagatgagca aacaagttcg
    53101 tgaaagtgaa ttggaagctt ttaaacaaga agcagaagag caaatccaat atttgaatgt
    53161 cacaccagca caacaacaaa tgattcgcta cttagtggat caaggtatcg tagtgaatga
    53221 cgtattgaat gagaaaaaga cagaagaatt gaaacaatcc gcacgagaag ctgtacaacc
    53281 agtgatgatc ttccaaggag aaatcattgt tcgtgaaggt aatcaaatcg atgcttcagc
    53341 tatgaaaaaa ttggaattac taggactgac taatcaaaca acttcgattt ttccgctaat
    53401 tgccatggtt ttagctgttt tgcttcaaat cggcgtactg atttataatt cacttcaatt
    53461 ccaaaatgca ggagaaagaa caaaatacgt gcttttctat gtgacagcta tgtcagtcag
    53521 catagtgctg atgaaatttt tccaattatt ccaaacggaa caagctactt atatcccgct
    53581 gttttatccc gctgcttttg caccacttgt tttaagcttt ttcttgaacc gaagagcagg
    53641 gatcatggct ggtattttcc aagcagtatc tgcacttttc attttttatg attcgattgg
    53701 cacgaacttt ctaacgatta tcttgatgtc ttatgttttc tctgggctca tggcaacgat
    53761 tgtcagacgc aaacggatct cagaacaagg attatctgct gccatgtggg tcattatttt
    53821 tcctgtgatg atggatgtca ttttgatcgt ctatcaagga atgagctttg gtgatgcaac
    53881 gacttggtta atgcttgtct gcgggttcac tggtgcactc ttctcattct tgctgacgat
    53941 cggtctacac ccatatattg aattgatggt caatgatgac agtgtaattg tattaaatga
    54001 actcagcaat ccaaatcatc ctttattgaa acagttgctg gaagaagcgc caggtactta
    54061 tcatcatagt atgatggtag ctaatctaag tgctaacgct gtagcagaaa tcggcggacg
    54121 ttctttgcta actcgagtag cttgctatta tcatgatatc ggcaagatca aacatgctag
    54181 ctttttcgtt gagaacttgc cttcaggagc ggaaaacccg cacaatttct tattgccaga
    54241 agacagtaaa catattatct ttggacatgt gacagatgga gccaaaatat tggaagatta
    54301 taatatgccg caaatggtga tcgatatttg caaacagcat catggaacca cgctaatgcg
    54361 ttatttctac atcaaagcga aagaacgcaa tccggaagta acagaagagc aattccgtta
    54421 tcctggacca aaaccgcaaa gtcgtgaagc agggatcgtc aatattgctg acagttgtga
    54481 ggctgcagta cgggcaatgg atcatccgac gagcgaaaaa atcgaaagtt ttgtccataa
    54541 tcttatcgaa gagcgtattt ccgatggtca attagacgat agtggactga ccttgaaaga
    54601 aatccgaaaa gtggaaaaat cattgatcag cggtttaagt agcaccttcc attctcggat
    54661 caaatatcct aaaatgaaaa cagaagttga gaagataaag gaagaacaag aagaatagaa
    54721 agaagcgatg tataatggat attaccttta tggatgaaac aaaccaagtc tcagaagaga
    54781 aaatgaaaga aatcgatgac ttgctgcaat ttgcagcatc ttatttaaag ctgccagagg
    54841 aaacagaaat gtctgttact ttcatggaca atgcggcaat ccaagtaatc aatcgtgatt
    54901 accgtggaaa agatgcaccg acagacgtca tcagttttgc tctggaagaa gaaggcgaag
    54961 acgaaatccc agtgattttt gaagatggag agaatccatt gcctcgtgta ctaggagaca
    55021 tcatgatttc tattgaacgt gctgctgaac aagcagaagc ttacggccat tcatatgatc
    55081 gggaacttgg ctttttagcg gtacatggat tcttgcatat caacgggtat gaccatatga
    55141 caccagaaga tgagaaagaa atgtttggtt tacagaaaga gatcttggat gcctatggac
    55201 tcaaaagata aaaacgtaga gaaaaacaaa catttcctga tgtctgtgga atttgctgtc
    55261 caaggagtca aaaccgtttt tgaggaagaa cgcaatatga ggaaacatgt tgcgttcggt
    55321 atccttgctt tatttatggg cgtgattttc cagcttgatc ggatagagtg gttatggctg
    55381 cttttagctg tttttctcgt ttggatagta gagatcatca atactgtttt tgaaaatgta
    55441 gtggacatgt ttacggattt tcattttcat ccgattggga aaaaaatcaa agatatggca
    55501 gctggagcag ttttagtgac cgcttgtttt gctgtcatca ttgggttgat tttatttgtc
    55561 cctaaaatat atcatttgtt gtttaattaa aaaggagaac gtatatgcca gaacataaat
    55621 cagggtttgt cgcaatcgtc ggccgcccga atgtaggaaa atccacatta ttgaaccgaa
    55681 tcgtgggtca aaaaattgcc attatgagtg acaaggcaca gacaactaga aataaaatcc
    55741 aaggagtata tactacacca gaagctcagc tgatttttat cgatacacca ggaatccata
    55801 aaccaaagca tcgtctagga gattttatgg tagaagcagc ttacagtgcg ttgaaagaag
    55861 tggatgcagt cttatttatg atcagtgctg atcaaaaacg cggccgggga gacgatttta
    55921 ttattgaacg tctaaaaaac gtgcattctc cagtttatct gattatcaat aaaatcgata
    55981 agatccatcc agacgagttg ctgggaatca tcgaagatta ttcgacgcag atgccttttg
    56041 cacaagtgat tccgatctct gctacagaag gaaacaatgt cgaacgctta atggatgtat
    56101 tagtcagtga aatgccagag ggcccacaat attttccaga tgatcaagtg actgatcatc
    56161 ctgaatactt tatcgtttct gaattgatcc gtgagaaagt cttgttttta acacgagacg
    56221 aagtgcctca ttctgtcgca gtcgttgttg attcgatgaa gcgaaacgag aatgataaaa
    56281 tccatatcca ggccacgatc atcgtcgaaa gagatagcca aaaaggaatc attatcggca
    56341 aaggcggaaa aatgctgaag caagtaggaa cgaaagcaag aaaggatatc gaaaatcttt
    56401 taggagacaa agtatttcta gaactttggg tcaaagtcca aaaagactgg cgagacaaaa
    56461 aagtctatct gcaagacttt ggctaccgaa aagacgaata ctgatattct gaacttctta
    56521 taggttaagt atagaaataa actggataaa aatccgcacg ttgagaaaaa cggcggaaga
    56581 tcgaaacata aataatctgg atcggcagaa tctgcttttc cagatttatt ttttaggagg
    56641 gagaatatga gccagcttgc agaatcgaaa ggcttgattt tatctagccg taattacaaa
    56701 gaaaaagata aattagtcaa aatctttacg gaatcttctg gcaaaatgat gttttatatc
    56761 aaaggaatcc atcggcaaaa tcagccatta gctccggctt tgctcccctt taccgaagca
    56821 gtctatatcg gtaattttcg aagtgaaggt ttgtcgtttt taagcagtgt caaagacgtc
    56881 catgcgtttc ggaacatcca agaagatatc tttttgagcg gttatggtac ctatttgcta
    56941 aatctagtag atgcagcaat cgaagatcat caatatgacc cgcatttatt ccaatttacg
    57001 caacaagctc tgcagcggat ggatcaagga gatgatgcag aaatcatcac gaatatcttt
    57061 gaagttcaaa tactgcaacg cttcggaatc gcgccaattt ggacgcactg tgtcgtatgc
    57121 ggggaaacaa aaggaaaatt tgactattct tccaaatctg gcggtgtgat atgcgagaaa
    57181 cactggccaa tggacgaaca ccgttatcat gcagatcctc gcgctgttta tttcgttcga
    57241 atgttttcgg ctatttctta tgataagatc agtggaatcc acttgaaaga agaaacgaag
    57301 caggcaatcc gtgacctcat tgaccagttg tatgatgaat atgtcggtat tcatctaaaa
    57361 agcaaaaagt tcattgatca aatgaagtct tgggcacacg tattaaagac gccagagaaa
    57421 aatatgcctg aaaaagagat acctgaaaaa gaagaataaa tgaaagatac aacaaaaaaa
    57481 tcaaaactat ttgacaaata cagggacttc cgtctattat taaccgtaga ttaaatagat
    57541 cgttcactga gaggatctat tatataaaca agaaaactgc aaaatgaata cgtacattga
    57601 ccaagatcag taaaaaacac gtggtttagc gagttcggga gagtggaagc cggacccaaa
    57661 cgatttttga agaggcactt gcgagtacga taaatgaagc tgccgaatct ttattcggaa
    57721 gtagggtgga accgcgaaat tattcgtccc tatgtctgta atcagacata gggacttttt
    57781 gtcgttttct ttcctgtatt cttattgaca gcaacgctta aaaattgaaa ggggcaaaac
    57841 agatgagtaa aaaattgacc gtccaagata tgattttaac attacaaaaa ttttggtcat
    57901 caaacggctg catgctgatg caagcttatg acacagaaaa aggagcaggt acgatgagcc
    57961 catatacatt cttacgggca atcggaccgg aaccatggaa tgctgcttat gtagagccat
    58021 cccgccgacc agctgacggg cgttatggag aaaacccaaa ccgtttgtat cagcatcatc
    58081 aatttcaagt cgtaatgaaa ccttcacctg aaaatatcca agagctttat ttagaaagct
    58141 tgcgtctttt aggaattgat ccattagaac acgatattcg ttttgttgaa gacaactggg
    58201 agaacccatc gatggggtgt gccggtgtgg gttgggaagt atggcttgat gggatggaga
    58261 tcacacaatt cacgtacttc cagcaagtcg gaggactacc ttgtaaacca gttacttctg
    58321 agatcactta tggactagaa cgtcttgctt catatattca agaagttgaa agtgtctatg
    58381 atctggaatg ggctgatggt gtgaaatatg gcgagatctt caaacaacca gaatatgaac
    58441 attccaaata ttcttttgaa gtaagtgacc aagacttgct actaagcaac tttgaccgtt
    58501 ttgaaaaaga agctaaacgc tgtatcgatg aaaatcttgt tcatccagcg tatgattata
    58561 ttttgaaatg cagccataca ttcaatttat tagatgctcg cggtgcagta tccgtaacag
    58621 aacgagcagg ttatctttcc cgtatccgca acatggctcg tgctgtagcc aaaatctttg
    58681 tggcagaacg cgaaaaatta ggattccctt tattgaataa aaacacagca aatccagaaa
    58741 acatgaagga ggctgaataa gatggcaaaa gacttattac ttgaaatcgg gctggaagaa
    58801 atgcctgctc atgttgtcac accaagcatg aaacaattag aagaaaaaac agcaaaattt
    58861 ttagatgaaa accaattgac ttatgatacg atcgaaacgt tttctacacc gcgccgctta
    58921 gcaatcaaag taacaaacat tccagaacgt caagcagaca gtgaagaaga agtcaaagga
    58981 ccagccaaaa aaattgcttt ggacgcagat ggaaactgga cgaaagcagc tgaaggattt
    59041 gtccgcggac aaggtctgac gacagatgct atcactttta gagaattaaa aggcgtggaa
    59101 tatgtctatg taacgaaata cactcacggc aaagaagcga aagaagtatt gacaggttta
    59161 gcagatgtca ttaaaagcct cactttccca gtaacgatgc attgggcaga atacgatttt
    59221 gaatatatcc gccctattca ctggatcgtg gcattgttag atgacgaagt aatcccgttt
    59281 gagatcttag atgtcaaaac aggcagagcc tctcgaggac atcgtttctt aggcgaagat
    59341 gtgacatttg cgcatgcaaa agaatatgaa gaaaaattga atgaacaatt cgttattgct
    59401 tcaccgcaaa aaagacaaga aatgatcgtt acacaagcaa atgaattagc cgaaaaaaat
    59461 cactgggtta ttgatctaga cgaagattta ctagaagaag tcaacaattt agtcgaatat
    59521 ccaactgtct tttctggcga cttcgaagaa aaatatcttt ctgttcctga agaagtacta
    59581 gtcacttcta tgaaagaaca tcaacgttat ttcgaggtac ggaacgatca aggtatgctg
    59641 cttcctcatt ttatttctgc tcgtaatggt gatcgggtaa aattggaaaa tgtggttcga
    59701 ggcaatcaaa aagtattgac ggctcgttta gaagatgcgg aattctttta taatgaagac
    59761 aagaaagctt caatcgacca atacgttgaa aaactaaaaa ctgttacttt ccatgaaaaa
    59821 atcggtagta tctatgagaa aatgcagcgg gtacaagtca ttgcccgtat cattggcgaa
    59881 caagtaggac tatctgagga agaattgatg gatctgcaac gagctgctga aatctataaa
    59941 tttgatctag taaccaacat ggtaggggaa tttcctgaac tacaagggat catgggcgaa
    60001 aaatatgctc tgctgaaagg cgagaaacct gctgtagcaa cagcgattcg tgagcattac
    60061 atgcctatat ccagtgaagg agagctacct cagtctacag taggttctgt tttagcgatt
    60121 gcagataaat tagatagcat tttttctttc tttgcagttg ggatgattcc aagcgggtca
    60181 aacgatcctt atgccttacg tcgccaagca tacggtgtcg ttcggatcat cgaatcgaaa
    60241 aaatgggcct ttcctttgtc tgttctgcaa gaaacgatta gtgatgttat cagtaaagat
    60301 acggatcgtt tcggcattgg tttatcagaa gggcaacaac aggtgatcga ctttatcaaa
    60361 ggccgtctgc gccaattatt gacaacgaag aatattcgtc atgatgtcat cgaagctgtt
    60421 ttgaatgcag aacaaaaaga tctgacaaaa gtatttgcag cagcacaatt gttcaagcag
    60481 catcttgcag atgaagactt caaaccatcg atggaagctt tgacacgtgt cgtgaactta
    60541 gcgaaaaaag cggagcttga aaaacaatca gaagtagacc ctgaactgtt cgaaaatgaa
    60601 gcggagaaag aactgcacaa agcagtagag gcaatgaaaa aaagctttgc tgaccaaacg
    60661 atcaacgaaa attatgatca gcttgtaagc cttcgcccac tgatcgaaaa ttattttgat
    60721 cagacgatgg tcatggcaga agaaccagca gtacgtaaga accgattaac gcaattaagt
    60781 caaattgctg ccatggcttt gtcgctagca agtttagatc aaatcatcac aaaataattt
    60841 ctaatatgta aataaaccag agaataggca caaaa
//
LOCUS       DAZUKZ010000005        60416 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000005 DAZUKZ010000000
VERSION     DAZUKZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.005"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..1535)
                     /locus_tag="ACG8EF_000292"
     CDS             complement(12..1535)
                     /locus_tag="ACG8EF_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS4945651.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="ACG8EF_000293"
     CDS             1787..2968
                     /locus_tag="ACG8EF_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4945652.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="ACG8EF_000294"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="ACG8EF_000294"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFS4945653.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="ACG8EF_000295"
     CDS             complement(5053..7764)
                     /locus_tag="ACG8EF_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFS4945654.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="ACG8EF_000296"
     CDS             complement(7774..8475)
                     /locus_tag="ACG8EF_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4945655.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="ACG8EF_000297"
     CDS             8661..9260
                     /locus_tag="ACG8EF_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS4945656.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="ACG8EF_000298"
     CDS             9341..10354
                     /locus_tag="ACG8EF_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFS4945657.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="ACG8EF_000299"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="ACG8EF_000299"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFS4945658.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="ACG8EF_000300"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="ACG8EF_000300"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFS4945659.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="ACG8EF_000301"
     CDS             11802..13175
                     /locus_tag="ACG8EF_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4945660.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="ACG8EF_000302"
     CDS             13532..15676
                     /locus_tag="ACG8EF_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4945661.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="ACG8EF_000303"
     CDS             complement(15728..16768)
                     /locus_tag="ACG8EF_000303"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4945662.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="ACG8EF_000304"
     CDS             complement(17045..18847)
                     /locus_tag="ACG8EF_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4945663.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="ACG8EF_000305"
     CDS             complement(18861..19613)
                     /locus_tag="ACG8EF_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4945664.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="ACG8EF_000306"
     CDS             complement(19622..19984)
                     /locus_tag="ACG8EF_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4945665.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="ACG8EF_000307"
     CDS             complement(20160..21032)
                     /locus_tag="ACG8EF_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS4945666.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="ACG8EF_000308"
     CDS             complement(21058..21570)
                     /locus_tag="ACG8EF_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS4945667.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22499)
                     /locus_tag="ACG8EF_000309"
     CDS             complement(21708..22499)
                     /locus_tag="ACG8EF_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFS4945668.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22554..23162)
                     /locus_tag="ACG8EF_000310"
     CDS             complement(22554..23162)
                     /locus_tag="ACG8EF_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFS4945669.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="ACG8EF_000311"
     CDS             complement(23155..23472)
                     /locus_tag="ACG8EF_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4945670.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="ACG8EF_000312"
     CDS             complement(23628..24356)
                     /locus_tag="ACG8EF_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFS4945671.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="ACG8EF_000313"
     CDS             24515..25396
                     /locus_tag="ACG8EF_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4945672.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="ACG8EF_000314"
     CDS             complement(25633..26676)
                     /locus_tag="ACG8EF_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4945673.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="ACG8EF_000315"
     CDS             complement(26679..27347)
                     /locus_tag="ACG8EF_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4945674.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="ACG8EF_000316"
     CDS             27489..29825
                     /locus_tag="ACG8EF_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFS4945675.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="ACG8EF_000317"
     CDS             complement(29940..30251)
                     /locus_tag="ACG8EF_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945676.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="ACG8EF_000318"
     CDS             30432..31907
                     /locus_tag="ACG8EF_000318"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFS4945677.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRNIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="ACG8EF_000319"
     CDS             31954..32538
                     /locus_tag="ACG8EF_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFS4945678.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="ACG8EF_000320"
     CDS             32540..32959
                     /locus_tag="ACG8EF_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFS4945679.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="ACG8EF_000321"
     CDS             complement(33240..33524)
                     /locus_tag="ACG8EF_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFS4945680.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="ACG8EF_000322"
     CDS             complement(33876..34586)
                     /locus_tag="ACG8EF_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFS4945681.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="ACG8EF_000323"
     CDS             complement(34583..36055)
                     /locus_tag="ACG8EF_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS4945682.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="ACG8EF_000324"
     CDS             36193..36666
                     /locus_tag="ACG8EF_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4945683.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="ACG8EF_000325"
     CDS             complement(36819..37370)
                     /locus_tag="ACG8EF_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4945684.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="ACG8EF_000326"
     CDS             37475..38005
                     /locus_tag="ACG8EF_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFS4945685.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="ACG8EF_000327"
     CDS             complement(38488..38931)
                     /locus_tag="ACG8EF_000327"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4945686.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="ACG8EF_000328"
     CDS             complement(38947..40107)
                     /locus_tag="ACG8EF_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4945687.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="ACG8EF_000329"
     CDS             40614..42314
                     /locus_tag="ACG8EF_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945688.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="ACG8EF_000330"
     CDS             complement(42416..43249)
                     /locus_tag="ACG8EF_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS4945689.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="ACG8EF_000331"
     CDS             complement(43553..45718)
                     /locus_tag="ACG8EF_000331"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFS4945690.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="ACG8EF_000332"
     CDS             45978..48272
                     /locus_tag="ACG8EF_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFS4945691.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="ACG8EF_000333"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="ACG8EF_000333"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS4945692.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="ACG8EF_000334"
     CDS             48923..49930
                     /locus_tag="ACG8EF_000334"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4945693.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="ACG8EF_000335"
     CDS             49952..50971
                     /locus_tag="ACG8EF_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4945694.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="ACG8EF_000336"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="ACG8EF_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS4945695.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /gene="tyrP"
                     /locus_tag="ACG8EF_000337"
     CDS             complement(52729..54150)
                     /gene="tyrP"
                     /locus_tag="ACG8EF_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFS4945696.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="ACG8EF_000338"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="ACG8EF_000338"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS4945697.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /gene="tyrS"
                     /locus_tag="ACG8EF_000339"
     CDS             complement(56561..57817)
                     /gene="tyrS"
                     /locus_tag="ACG8EF_000339"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFS4945698.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57929..58134)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58397..59698
                     /locus_tag="ACG8EF_000340"
     CDS             58397..59698
                     /locus_tag="ACG8EF_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS4945699.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="ACG8EF_000341"
     CDS             complement(59767..60366)
                     /locus_tag="ACG8EF_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4945700.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        18227 a        12363 c        10827 g        18999 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgcaacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaat
//
LOCUS       DAZUKZ010000006        53837 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000006 DAZUKZ010000000
VERSION     DAZUKZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.006"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..1784
                     /locus_tag="ACG8EF_000342"
     CDS             153..1784
                     /locus_tag="ACG8EF_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFS4945701.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1846..2487)
                     /locus_tag="ACG8EF_000343"
     CDS             complement(1846..2487)
                     /locus_tag="ACG8EF_000343"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS4945702.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    2673..2749
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            2769..3077
                     /gene="rplU"
                     /locus_tag="ACG8EF_000344"
     CDS             2769..3077
                     /gene="rplU"
                     /locus_tag="ACG8EF_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFS4945703.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            3104..3448
                     /locus_tag="ACG8EF_000345"
     CDS             3104..3448
                     /locus_tag="ACG8EF_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFS4945704.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3461..3754
                     /gene="rpmA"
                     /locus_tag="ACG8EF_000346"
     CDS             3461..3754
                     /gene="rpmA"
                     /locus_tag="ACG8EF_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFS4945705.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4188..6995
                     /locus_tag="ACG8EF_000347"
     CDS             4188..6995
                     /locus_tag="ACG8EF_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4945706.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7186..7677
                     /locus_tag="ACG8EF_000348"
     CDS             7186..7677
                     /locus_tag="ACG8EF_000348"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS4945707.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7780..8769
                     /locus_tag="ACG8EF_000349"
     CDS             7780..8769
                     /locus_tag="ACG8EF_000349"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS4945708.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            8802..9605
                     /locus_tag="ACG8EF_000350"
     CDS             8802..9605
                     /locus_tag="ACG8EF_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFS4945709.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9626..10540
                     /locus_tag="ACG8EF_000351"
     CDS             9626..10540
                     /locus_tag="ACG8EF_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4945710.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10623..10997
                     /locus_tag="ACG8EF_000352"
     CDS             10623..10997
                     /locus_tag="ACG8EF_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFS4945711.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11136..11381
                     /locus_tag="ACG8EF_000353"
     CDS             11136..11381
                     /locus_tag="ACG8EF_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4945712.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11553..12617
                     /locus_tag="ACG8EF_000354"
     CDS             11553..12617
                     /locus_tag="ACG8EF_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFS4945713.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            12863..13288
                     /locus_tag="ACG8EF_000355"
     CDS             12863..13288
                     /locus_tag="ACG8EF_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4945714.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13281..13730
                     /gene="nusB"
                     /locus_tag="ACG8EF_000356"
     CDS             13281..13730
                     /gene="nusB"
                     /locus_tag="ACG8EF_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFS4945715.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            13827..14681
                     /locus_tag="ACG8EF_000357"
     CDS             13827..14681
                     /locus_tag="ACG8EF_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFS4945716.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            14682..16019
                     /gene="xseA"
                     /locus_tag="ACG8EF_000358"
     CDS             14682..16019
                     /gene="xseA"
                     /locus_tag="ACG8EF_000358"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFS4945717.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            16024..16248
                     /locus_tag="ACG8EF_000359"
     CDS             16024..16248
                     /locus_tag="ACG8EF_000359"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFS4945718.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16250..17140
                     /locus_tag="ACG8EF_000360"
     CDS             16250..17140
                     /locus_tag="ACG8EF_000360"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS4945719.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17149..17967
                     /locus_tag="ACG8EF_000361"
     CDS             17149..17967
                     /locus_tag="ACG8EF_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFS4945720.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            18074..18523
                     /locus_tag="ACG8EF_000362"
     CDS             18074..18523
                     /locus_tag="ACG8EF_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4945721.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            18541..20223
                     /gene="recN"
                     /locus_tag="ACG8EF_000363"
     CDS             18541..20223
                     /gene="recN"
                     /locus_tag="ACG8EF_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFS4945722.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20278..20394)
                     /locus_tag="ACG8EF_000364"
     CDS             complement(20278..20394)
                     /locus_tag="ACG8EF_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFS4945723.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(20467..21423)
                     /locus_tag="ACG8EF_000365"
     CDS             complement(20467..21423)
                     /locus_tag="ACG8EF_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4945724.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21594..21962
                     /locus_tag="ACG8EF_000366"
     CDS             21594..21962
                     /locus_tag="ACG8EF_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFS4945725.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            22132..22563
                     /gene="mraZ"
                     /locus_tag="ACG8EF_000367"
     CDS             22132..22563
                     /gene="mraZ"
                     /locus_tag="ACG8EF_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFS4945726.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            22584..23543
                     /gene="rsmH"
                     /locus_tag="ACG8EF_000368"
     CDS             22584..23543
                     /gene="rsmH"
                     /locus_tag="ACG8EF_000368"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFS4945727.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            23548..23949
                     /gene="ftsL"
                     /locus_tag="ACG8EF_000369"
     CDS             23548..23949
                     /gene="ftsL"
                     /locus_tag="ACG8EF_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFS4945728.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            23950..26142
                     /locus_tag="ACG8EF_000370"
     CDS             23950..26142
                     /locus_tag="ACG8EF_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS4945729.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            26168..27130
                     /gene="mraY"
                     /locus_tag="ACG8EF_000371"
     CDS             26168..27130
                     /gene="mraY"
                     /locus_tag="ACG8EF_000371"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFS4945730.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            27144..28505
                     /gene="murD"
                     /locus_tag="ACG8EF_000372"
     CDS             27144..28505
                     /gene="murD"
                     /locus_tag="ACG8EF_000372"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFS4945731.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            28531..29619
                     /gene="murG"
                     /locus_tag="ACG8EF_000373"
     CDS             28531..29619
                     /gene="murG"
                     /locus_tag="ACG8EF_000373"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFS4945732.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            29634..30854
                     /locus_tag="ACG8EF_000374"
     CDS             29634..30854
                     /locus_tag="ACG8EF_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFS4945733.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            31010..32335
                     /gene="ftsA"
                     /locus_tag="ACG8EF_000375"
     CDS             31010..32335
                     /gene="ftsA"
                     /locus_tag="ACG8EF_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFS4945734.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            32358..33599
                     /gene="ftsZ"
                     /locus_tag="ACG8EF_000376"
     CDS             32358..33599
                     /gene="ftsZ"
                     /locus_tag="ACG8EF_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFS4945735.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            33616..34293
                     /locus_tag="ACG8EF_000377"
     CDS             33616..34293
                     /locus_tag="ACG8EF_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFS4945736.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            34307..34906
                     /locus_tag="ACG8EF_000378"
     CDS             34307..34906
                     /locus_tag="ACG8EF_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFS4945737.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            34987..35208
                     /locus_tag="ACG8EF_000379"
     CDS             34987..35208
                     /locus_tag="ACG8EF_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFS4945738.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            35257..36039
                     /locus_tag="ACG8EF_000380"
     CDS             35257..36039
                     /locus_tag="ACG8EF_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFS4945739.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            36060..36767
                     /locus_tag="ACG8EF_000381"
     CDS             36060..36767
                     /locus_tag="ACG8EF_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFS4945740.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            37029..39827
                     /gene="ileS"
                     /locus_tag="ACG8EF_000382"
     CDS             37029..39827
                     /gene="ileS"
                     /locus_tag="ACG8EF_000382"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFS4945741.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(39876..41402)
                     /gene="zwf"
                     /locus_tag="ACG8EF_000383"
     CDS             complement(39876..41402)
                     /gene="zwf"
                     /locus_tag="ACG8EF_000383"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFS4945742.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(41417..42064)
                     /locus_tag="ACG8EF_000384"
     CDS             complement(41417..42064)
                     /locus_tag="ACG8EF_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS4945743.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42248..42577)
                     /locus_tag="ACG8EF_000385"
     CDS             complement(42248..42577)
                     /locus_tag="ACG8EF_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFS4945744.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            42754..43482
                     /gene="sfsA"
                     /locus_tag="ACG8EF_000386"
     CDS             42754..43482
                     /gene="sfsA"
                     /locus_tag="ACG8EF_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFS4945745.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            43498..44511
                     /locus_tag="ACG8EF_000387"
     CDS             43498..44511
                     /locus_tag="ACG8EF_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4945746.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            44511..45788
                     /locus_tag="ACG8EF_000388"
     CDS             44511..45788
                     /locus_tag="ACG8EF_000388"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4945747.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            45851..48553
                     /locus_tag="ACG8EF_000389"
     CDS             45851..48553
                     /locus_tag="ACG8EF_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4945748.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            48705..49022
                     /locus_tag="ACG8EF_000390"
     CDS             48705..49022
                     /locus_tag="ACG8EF_000390"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4945749.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            49052..49372
                     /locus_tag="ACG8EF_000391"
     CDS             49052..49372
                     /locus_tag="ACG8EF_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4945750.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            49480..50940
                     /locus_tag="ACG8EF_000392"
     CDS             49480..50940
                     /locus_tag="ACG8EF_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4945751.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            51008..51229
                     /locus_tag="ACG8EF_000393"
     CDS             51008..51229
                     /locus_tag="ACG8EF_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945752.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51260..51442
                     /locus_tag="ACG8EF_000394"
     CDS             51260..51442
                     /locus_tag="ACG8EF_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFS4945753.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            51442..51855
                     /locus_tag="ACG8EF_000395"
     CDS             51442..51855
                     /locus_tag="ACG8EF_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFS4945754.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            51978..53159
                     /locus_tag="ACG8EF_000396"
     CDS             51978..53159
                     /locus_tag="ACG8EF_000396"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4945755.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(53230..53394)
                     /locus_tag="ACG8EF_000397"
     CDS             complement(53230..53394)
                     /locus_tag="ACG8EF_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945756.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            53548..53621
                     /locus_tag="ACG8EF_000398"
     tRNA            53548..53621
                     /locus_tag="ACG8EF_000398"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53582..53584,aa:Arg,seq:cct)
BASE COUNT        17773 a         9129 c        11633 g        15302 t
ORIGIN      
        1 ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag
       61 gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt
      121 ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa
      181 aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca
      241 cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg
      301 cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg
      361 gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg
      421 caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg
      481 cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg
      541 ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg
      601 ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc
      661 cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc
      721 caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag
      781 ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca
      841 gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc
      901 cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg
      961 gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa
     1021 atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg
     1081 tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac
     1141 tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag
     1201 gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta
     1261 ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct
     1321 ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca
     1381 aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag
     1441 aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt
     1501 cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct
     1561 ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag
     1621 aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa
     1681 aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg
     1741 cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac
     1801 agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa
     1861 aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa
     1921 aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc
     1981 atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt
     2041 aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa
     2101 ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga
     2161 acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg
     2221 atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt
     2281 taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac
     2341 atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag
     2401 aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc
     2461 ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat
     2521 aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt
     2581 attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt
     2641 ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta
     2701 ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc
     2761 tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac
     2821 aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag
     2881 ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg
     2941 ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac
     3001 ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg
     3061 acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt
     3121 taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg
     3181 tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa
     3241 cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg
     3301 ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat
     3361 tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat
     3421 ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct
     3481 acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc
     3541 aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta
     3601 ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac
     3661 tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca
     3721 agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt
     3781 gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag tggaggctac
     3841 gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat caggatgttt
     3901 tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg aaataattat
     3961 atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg atttttcgtt
     4021 cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa tcaggtaaat
     4081 agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt acaatagtct
     4141 atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg tatgtcaaag
     4201 aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca caggaaattg
     4261 ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag ctagtgcgcg
     4321 aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa acactcgtta
     4381 cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg gtctctgaag
     4441 aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt ggagctaacg
     4501 gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg ccaagaggat
     4561 taaattgttt gattacagga cctaccggat caggaaagac ttattttgca cacgcgatgt
     4621 atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt gttttcaatt
     4681 gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg tacgcaaaag
     4741 gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg gatggcggta
     4801 tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg atcttttact
     4861 ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat gaagcaaatg
     4921 tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa acttttgttc
     4981 ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca aatgagaaag
     5041 tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga aagatttctt
     5101 tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat ataggacaat
     5161 tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg caatcacccg
     5221 aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg attcaattag
     5281 caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag ataactgtag
     5341 caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct tataatctat
     5401 atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat caagaagcaa
     5461 tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat aaagaccatg
     5521 gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa atcatagaag
     5581 tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt cagcaaaact
     5641 ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac ttaggagaag
     5701 aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat gaatatgctg
     5761 ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt ccagaaagtg
     5821 aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca agcggcagaa
     5881 tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc caagttgtcc
     5941 aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat atggatccga
     6001 aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga agcggggtca
     6061 tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa agagacactg
     6121 gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa actgtacgaa
     6181 aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa aattttcgag
     6241 gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt cataagaaaa
     6301 aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc aaagaattga
     6361 tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct ttgtcgattg
     6421 tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata gctactactg
     6481 ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt attgatcaaa
     6541 atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa actgaattca
     6601 tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct gataacttca
     6661 tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca caaatctctc
     6721 agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg atgcacacag
     6781 caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa aacgaattga
     6841 caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt ctgttggaag
     6901 atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt ctagtccata
     6961 atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat acacttttat
     7021 agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg tatcgtaaaa
     7081 aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt cacacagaaa
     7141 tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga tattcgattg
     7201 gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc gaagcaaaca
     7261 ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt gagaaagttt
     7321 ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt gcagaaaatg
     7381 attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt attcaccaat
     7441 ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt gaatatcggc
     7501 ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt aaatcaacaa
     7561 gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat tcgaaaagta
     7621 cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa ggtgtagcaa
     7681 gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt attaatattt
     7741 ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat tatcctagca
     7801 agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt tggcgaacaa
     7861 gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt gagagccaaa
     7921 ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt agttgattta
     7981 tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa agataaatgg
     8041 gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc acgcttttca
     8101 atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga cggtgtaaaa
     8161 gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga agacgcacaa
     8221 cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa atatgtatta
     8281 gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac aaaagcagtg
     8341 caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct gcgtaagaga
     8401 ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat tagcaaaatg
     8461 atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt atttgaaaat
     8521 cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt aaatgttggg
     8581 tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat gggacaagaa
     8641 gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt acgtaaagta
     8701 cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc tgaattagca
     8761 aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt atttcaataa
     8821 ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat gaatttcaat
     8881 ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac ctcgaagctg
     8941 gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat atcggtgccg
     9001 ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta gttttaggtg
     9061 gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg gctgttgcag
     9121 ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat ttgatggatg
     9181 cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt gctgtatgtt
     9241 tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca gcacaaactg
     9301 tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct atcggtgggg
     9361 gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca aaagaagtat
     9421 ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca ttgattgctc
     9481 taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa atgggtggtt
     9541 catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt ttgaatgact
     9601 attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat caaaaaaaga
     9661 tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact acgaacgtat
     9721 gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat acaaaacaaa
     9781 agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac acccatacat
     9841 tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga atggcgcgcc
     9901 tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt tagctggtgt
     9961 tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag gtgcttcatt
    10021 agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga acattattcg
    10081 ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta aaattactga
    10141 tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt tagggatgtt
    10201 cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag ttgtatcaac
    10261 tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag gtggtgaagg
    10321 aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg aaaaggtaac
    10381 aacattacaa gacaacttag accaattgat tcctggttta gctgcattag gattaacatt
    10441 gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc ttggtttatt
    10501 cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa tatagctcgg
    10561 gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc tggagggaga
    10621 aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact tcgtttacgg
    10681 gtctgacaga ttacgggcaa atcatgatag gtgataatgg ttttgagttt taccatgcgc
    10741 gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta ctagcatcag
    10801 tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa aatggtacat
    10861 acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa tatgtagatg
    10921 aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt attcgtagta
    10981 tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct ttattttttt
    11041 actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt aaatcccata
    11101 tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg acattgattg
    11161 ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat tcgatgggct
    11221 gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg attttcggcg
    11281 aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc gcagtgattt
    11341 tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca aaatagtaaa
    11401 ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt aaaataaaaa
    11461 gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg ctataatgga
    11521 aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa aaattgcgca
    11581 aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac aatcttcgtt
    11641 acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa aaagcatttt
    11701 ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg tttgaaatta
    11761 ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa gaagaactgc
    11821 actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt ttagaagaaa
    11881 tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga gaagtgaaag
    11941 atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg gcatatgatc
    12001 acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat caactagact
    12061 tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt gctagcggat
    12121 tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa ggtgacttga
    12181 tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca cgtacattcg
    12241 ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta gatgctcaat
    12301 tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat gcagttgcta
    12361 gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca ggacatggga
    12421 tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag caatttgtac
    12481 caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt ggagtacgta
    12541 ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt tcaccaaaag
    12601 aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca gtgttcacag
    12661 actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt ttaagctgta
    12721 agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg atcggcggca
    12781 gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta tctcgtgctt
    12841 aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa acgcaaacca
    12901 agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg ggattgcagc
    12961 ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg tcactgaatt
    13021 attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag atggcttgaa
    13081 agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag caatggaaat
    13141 gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat ctgaagtcaa
    13201 tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg aagaactttt
    13261 tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt cgggaaatgg
    13321 cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa gatgcgattt
    13381 tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca gagtttgttc
    13441 ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa ttggataaag
    13501 tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa atggatttgg
    13561 tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca gcggctgttg
    13621 ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca cgtaaattcg
    13681 tcaacggtgt tttatccaat gtattgaaag aattagaagc tggggcgtaa gtcctagctt
    13741 ttttctatga atcatcataa acctgttata aatcgggaaa attctatggt agaatgaaaa
    13801 cacaaaaaat atctgggagt ggttagatgg cagaattgat taacggaaaa gaattagctg
    13861 aaaaaatgca ggcagagatt gctgaaaaaa tccaaaaatt gaaagataat ggtatccatc
    13921 ccggattagt cgttttattg gtaggggaaa atccagccag ccaaatctat gtaagaaata
    13981 aagaacgtgt agcaaaagaa atcggaatcc attcattggt agagcgttat cctgagacga
    14041 tttctgaaga cgaactactt gcagaaattg aaaaatacaa tcaagatcct agttttcatg
    14101 gtatattggt ccaattacca ttgccaaagc atatagatga agaaaagatc ctgttagcta
    14161 tccgccctga aaaagacgta gacggttttc atccgatgaa tttaggcaga ctccttgcgg
    14221 gtaaaccgga caagattcct tgtactccat acggcatcat gaaaatgttt gaagcttatt
    14281 ctatcgatcc ggcgggtaaa cgagcagtag ttatcggacg aagcaatatc gtcggtaaac
    14341 cgatggctca gctactcttg atggcagatg caactgttac tgttgctcat tctaaaaccg
    14401 aaaatctggc tgaactggca caagaagcag atattttagt cgtagcaata ggacgtggac
    14461 attttgtcac aaaagaattc gtaaaaccag gtgctgtagt gatagatgta gggatgaatc
    14521 gtgatgctaa cggtaagctg atcggtgatg tgaaatttga tgaagtggag cctattgcag
    14581 gctggatcac tcctgtgcca aagggtgtag ggccaatgac tatcacgatg ctgatgtatc
    14641 aaaccgtaaa atctgccgaa gcttttggag caggtgaata gatggcacaa gaatatttaa
    14701 ctgtcactac attaacgaag tatttgaaaa gaaaatttga tgccgatcct tatttagaac
    14761 gcgtatattt aacaggcgaa atatcgaatt tccgtttacg tgccaatgcc catcaatatt
    14821 tcagcttaaa agatgaccat gcaaaaatat ctgctattat gttcaaagga gcattccaaa
    14881 aattacgatt ccagccaaaa gaaggaatga aagtccttgt cgttgggcgt atttcattat
    14941 atgaagcggg aggttcttat caaatctatg tagagcacat ggagcctgat ggaataggtg
    15001 ctttatatca agaattggaa gaacgaaaag aaaagctgat gaaggaaggc ctttttcaag
    15061 ggcctaaaaa aatgctgcca cgtttcccaa agcgaatcgc ggtccttacc agtccaagcg
    15121 gagcggttat ccgcgatatc atcacgacgg tgaaaagaag atatccaatt gtccaattgg
    15181 ttttgttccc aacagttgta caaggaaacc aagcagcgga caatgttgtc aggaatatcc
    15241 agagagtaga agagatgggc aatttcgata caatgatcat cggtcgtggg ggtggttcga
    15301 ttgaggattt atggccgttt aatgaagagc gggttgctcg ggcgatttat caagctaata
    15361 cgccggtgat ttcatctgta ggacatgaaa cagataccac gatcgctgat ttagtagcag
    15421 atgttcgggc tgccactcca acagcagctg cagaattagc tgttcctgtt ttgtcagaag
    15481 agttgatgcg cattcaagaa cggcaagcta gattgcagca ggcgtttgta cgccagatcc
    15541 agcgcaaaca agaacggttc gagcgtgctc gacgttccta tgtatttcgg cagcctgaaa
    15601 gattgtatga aggtcagacc atcaaattag accagttgaa acagcgatta tatcaagcta
    15661 cacagcaaat ttatcatgcg aaagaaaaac aaacgatcgc attaagccac caattggaac
    15721 aagtggctcc gctttatcga gttaaagcag ctcgccaaga aactgcttat ctagaaaaac
    15781 gcttagaaga gaaaatccaa caatatatga aacaacagca gcagcgtttt gctcaagctg
    15841 ttcaatctct tgatttgttg agtccattga aaatcatggg aagaggctac agctatacga
    15901 cgttaaatga tcaagtagta aaaagtgtca ccgagattca tcaaggggat ttgctgacga
    15961 tccattatca agatggaatc gttaaaggac aagtacaaga gatagacaaa ggagaataaa
    16021 aatatggaaa atccaacttt tgaagagtca ctgcaagaat tagaaaaaat tgtcatgcag
    16081 ttagaacaag gagacgttcc attagaatca gctctagatt cgtttaaacg aggaatggaa
    16141 ttaagcaaac attgccaaga tacattagct aaagcagaag agaccttgac gaaaatgatg
    16201 acagaatcaa atgaagaagt tgattttgac ggaaacgagg aaagctgaga tgagattaac
    16261 tgacttttca gctattcatc tacctgctgt agaaaaagaa atcttgtctt ttttagatga
    16321 gcatacaacg gatagaggac tctatgatgc gatgagttat tcagtgaaag caggcgggaa
    16381 aagaatacgt cccttattac ttttaacggc cgttgcttcc tttgatgagc ctattgatgt
    16441 tcccgtctat caagtagcag ctgctttaga aatggtgcat acgtattcct tgatccacga
    16501 tgatttgcct gcgatggaca atgatgatct tcgtagagga aaacctacca atcataaagt
    16561 attcggcgaa gcattagcta tattggccgg agatggtttg cttacaggag cattccagct
    16621 aatcagtatg gcacacttag gtaattctcc taagcttctg ctgcttcagc aattagctgt
    16681 ttgtgcggga tcacaaggta tggttgctgg gcaagctgca gatatcgagg gcgaatcgaa
    16741 aaagctttct ttagaagaat tagcatttat tcatgaaaga aagacagggc atttgattcg
    16801 ttatgccttg ctggctggtg gcatattagc gaagcaacct gaagaaatct tattgctact
    16861 tcaacgatta gccgaacatt taggtttagc ttttcagata agagatgatc tactcgatgt
    16921 gattggtaca acgaaaacat tagggaaaac agctggtaaa gatgaacgta tggaaaaaaa
    16981 tacttacccg cgtttattag gcctagaaaa gacacgtgag gcgttggaga ttgagctgtt
    17041 atccgccaac aagatcatag acaaattaga agaggacgtt ttttcttttg atggcgggct
    17101 gatgcgccag atgatcaagc aatttgatgt ggagaaatga gggaaagaat ggaaaaagaa
    17161 cgcgtagatg tattagcggt caaacaagga ttatttgaaa caagagaaca agccaaacgt
    17221 tcagtgatgg ctggtttgat ttacaacgaa aaaaacgagc ggtttgataa accgggagaa
    17281 aaaattccag tgagtagtga attaagagtc aaaggtaaaa aacttcctta tgtttctcga
    17341 gggggattaa aattagaaaa agcactcaaa gcatttgatc tatctgttga aggaaaaaca
    17401 ttgttagata ttggtgcatc cactggcgga tttactgatg cagctttgca aaatggcgca
    17461 aaaatgagtt atgctttaga cgttggctac aatcagctag cttggaaaat aagacaagat
    17521 cctagagtcg tcgtaatgga aagagtgaat tttcgttatg caaaacccga agactttacc
    17581 ttaggaattc cagaagttgc tgttatcgat gtttctttta tttcactaaa actgatgctg
    17641 cctccattac atgctatttt aaaagaagag ggagaagtga ttgctttaat caaaccccaa
    17701 ttcgaagcag gcagagaagc tgtagggaaa aacggaattg ttcgtgaccc tcagacccat
    17761 caaaaagttt tagaagacat tttgtcattt gcggcacaag gtggatatga tgtgctggaa
    17821 ttaagttatt ctccgatcac ggggggagaa ggaaatatcg agttcttggc tcacttgagg
    17881 aaagtacccg aaagcggaac aatcaattca gctattaata tggcagaagt ggtttcaaat
    17941 gcacacgaac agtttgacca taaataaatt gtaactgtga aaatcaagtg agaagtatgc
    18001 ggattcttct ttattatacc aaggaaataa gctatcattg attaagaaga atatgctaag
    18061 gagcgaagac gtaatgagaa aaaaagatcg gcatcgtttg attactcgat tgttgagtga
    18121 gcaagatatc cgaaagcagg aagaatttgt tgaaatcctg aaaagcaaag gaatatctgt
    18181 tactcaagct acgatttcca gagatataaa ggaactgaaa ctgatcaagg taccggcttc
    18241 agatggcggc taccgttata gcataccagc tgaaacaaac gaagatgtaa atactaaatt
    18301 ggaaaaacta ttaaaagacg ctttcgtggc agttgaccaa atggaaaaat ttgttatttt
    18361 gaaaacattg ccgggaaatg cttcggcagc agcaaatttg attgataaac gttacaagaa
    18421 agaattattt tcgattatca atgatgacga taatgttttg atgattacac gaactgaaga
    18481 ccaggcagtt ttattgaaga aagacttcct gcattacctt taagttgagg agcgaatgtg
    18541 atgctacaag aaatcagtat tactaacttt gcgattattc cggaattgcg gctttctttt
    18601 cacgaaggaa tgaccgcgct gaccggagag actggtgcgg gtaaatccat tattatcgat
    18661 gctttaggtc tgttagcagg aggaagaggt tcaagcgatt atatacgtca aggagcagaa
    18721 aaatgtgtgc ttgaagggtt gtttgaacta ccaaaacaag aaggattctc tgaactgatg
    18781 gttgaattag ggatcgaaac agatgaggac aatctgattg ttcgacggga tatgtcctta
    18841 actggaaaaa atgtttgtcg agtgaatggt catattatca cattagctaa tctgagaaga
    18901 atcggcagtt atttagtaga tatccaagga caaaacgaac atcaggaact gctccagcct
    18961 gaatcacatc ttgctttatt ggatcgtttt ggagatgtag ctttccaaca gaagaaaaag
    19021 agttatcagc aagaatatat ctcttatcgt gagctggaaa aaagggtacg aaaagtgcag
    19081 caaaatgaaa aatcctatgt tcagagaata gatatgctcc atttccagca agaagaaata
    19141 gctgctgctg atttgcaagt cggagaagaa gagaaattaa aagaagaaag agaaaaactt
    19201 agcaattatc aaaaaattgc tgatggatta gctgcaggtt acggagcact gacgaataat
    19261 gaacaaaaca gtgtagatgg tgttggcttg gccgtttcag aactgcaagg gatcgcccat
    19321 ctagatgtgg agtatgaagc aatttatgaa aatatccaaa gtgcttacta tttgctgcaa
    19381 gatgcaatcg gcgatatgag tcgtcagatt gatttattgg agttagatga gagtcgttta
    19441 gaagaagtta cgcaacgttt agaattaatc cgacagttga aacgtaaata cggcgaatcc
    19501 attgagtcaa tattggctta ctatgacgaa atcaccgaag agctagcttc ttctgatttt
    19561 tcagaaggtc aattggacaa aatgaaagag gaactagagc aaaaagagct tctacttcag
    19621 caacaagccg cagatcttca tgaagcgcgt aaaaaaatcg caaaagaatt ggaaaaatcc
    19681 attctgcatg aattgaaaag tctgtatatg gaaaacactg agtttgaggt acgtttctta
    19741 aaagaagaaa atagacaatt gaatcatgat ggatttgacc aaatcgaatt ttacattacg
    19801 accaatccag gagagccact gaagcctttg gtgaaagtcg cttctggtgg cgaactgtct
    19861 cggatgttgc ttgctttgaa aactattttt tcttctgagc aaggtgtgac aagtattatt
    19921 tttgatgaag tggatacagg tgtgagcgga agagttgcac aggccatcgc agataaaatt
    19981 tctcaaatat cgaaatactc tcaagttttg tgcatcactc atctgcctca agttgctgca
    20041 gtcgccgact atcaatatta tattgtgaaa gcagtgattg atggacgtac gcaaacttct
    20101 gtttcagagt tgaaaacaaa agaaagagaa gaagaaattg cacgtatgct ggctggaagt
    20161 gagattacaa aactcacact cgaacatgca aaagaactat tgaaactcgc aaaaaggacc
    20221 tgagccacct cgcagtggtt caggtccttt tcagcagctg atagctatat tttattttta
    20281 catgacactc attacagcag tcaatacaac agatccaatc gttaaaatca gcattgtcca
    20341 aatgacaaat tttgttactt tagcaaaagt acttgtttgt tttttttcac gcattgtttt
    20401 caacctcgtc attaattagt tgattcaagt gtacaatctt ttttccattt ccacaagagg
    20461 gcttttttat aaaaacttcc ctttttttaa ataataggta gttacgatac aaataaataa
    20521 tgttaataga actagccata taaaggcatc atccctttca gcaataggta acttaatgtt
    20581 cattccatag atactgccga cgattgtcgg tatagttaaa ataatcgtta aagaagttaa
    20641 aattttcata atattattca gattattcga tacgattgct gaaaaagtct gattcatttg
    20701 ttctactagt tttaattgaa tgcgagaagt agtcattgcc tgctctgttt ccaccaatac
    20761 atcacgtaaa tgatttttca aagcatgatt ttcttggaaa agtttatttt gcgctagttc
    20821 tttcaatgca tctaaattag cagaagtcgc ttctctgaaa taaacgaggc ttttttggat
    20881 atccatgatt tgataaagtt gtttgttttc agtggatact tgaagtttgg cttccaaagt
    20941 atcgcattga gattgaagcg tagctaaata actgttgtaa gagagtgtga cctgccaaat
    21001 gaattgaaag aagatattga gattaggcga aatatcactt tttggaaagg tctggctaaa
    21061 gactttcttt aaaaacagtg gttcatgatt cgtaattgtg ataagcttct ttttgggcgt
    21121 caaaataata gagagcgggt atgtatccac ttgaaaataa ccgctaggac ttgttttagc
    21181 aaaaggatat tgtaacaata ataacgctgg ctttgtcaac tttgattgat tgaattcttc
    21241 tgtacgggaa ttttctgcat cgtccaatat ggaagtgatg taatctgacg gaatttcaaa
    21301 ttgatgtgtc agctgatgaa tctcttcttg attaggacgt tctacacaat accagatcgt
    21361 ttggtcagtt tcctttccgg tattacgcag tttctcatta tctaatgtaa aatatttgat
    21421 catgattttc ctcctgttgc ctgtttcttt tattataacg gatagggaat tggaatacat
    21481 ggaatgtgtt tgtaagatat agaatataac tgcataaatt taaataaaat gaaacagttg
    21541 tgtaaagtat atacagtata cttgaagcaa cgataaaaaa ggagtttacg ttcatgggca
    21601 cattcacacc acttatcttg ttttggtata tttacccggt aatcgttttg tttggttgcc
    21661 aatttcttgt aaaattattt tcgttaaatc gtcgttttaa aataaaagcc cctgatttgg
    21721 cgattccttt tttgtggggc ggtatgcatg cattgtctag aaatacaggg acaatttcta
    21781 tcttaccgtt tttctttatt tctgttttgc taatgggaat ttgtattgct gttttccaag
    21841 catattatta tgaagaaatt atttatccgc gttatttcaa gatgacttgg cgtttggttt
    21901 ttctgttgac aatcgtcttg tacgctgtct tgatcattgt gaacatactt tcttacctat
    21961 gaaaaaaata gaaaagctgt aaaaagtctt ttaaaaggct ttttgcagct tgatttttaa
    22021 aaaaatgttt atgaataaaa aaagttgttc ccggatttcc cagaaaaggt ggtagaaagt
    22081 gggggattgt ggtagactaa ttttagttag tggaggaatg gggggctttc aatgttgatg
    22141 ggtgaattcc aacataatat tgatgcaaaa ggacgtttaa tcgtgccctc aaaacttcgt
    22201 gaagaacttg gcgaaaagtt tgttctgaca cgcggattgg atggctgtct tttcggctat
    22261 ccgatgagcg aatgggaaaa tctggaagct aaactgaacg aaatgccgtt ggctaaaaaa
    22321 gacgcacgta cttttgtgcg attcttctat tcggctgcca ctgaatgcga acttgataaa
    22381 caaggacgaa tcaatatacc cagcacgtta agaaattatg cagctttgac aaaaggttgc
    22441 gtagtcatcg gtgtatctaa ccgaattgaa atttgggatg agacacgatg gcaagatttt
    22501 tctgcagcag cagaagagaa ctttgacgaa attgctgaaa gtatgattga tttcggacta
    22561 taagaatagg aagaggctga ctaatgactg aaacatttca acactataca gtcatgttga
    22621 aagaaacggt agatggattg aacatcaaac cggatggcgt ttatgtagac tgtacactcg
    22681 gcggtgccgg acatagtgaa tatttattaa gccaactaag ttctaaaggg catctgtatg
    22741 cgtttgacca agatcaaaaa gcaatcgatc acgccaagct gcgtttagct tcttatgtcg
    22801 aacaaggtat ggtcacattt atcaaagcga acttcagaga attagaagaa cgcttgagtg
    22861 agcatgtaga taaggtagac ggcattttgt atgatctagg ggtttcctct cctcagctag
    22921 atgaagcaga acgtggattt agttatcatc aagatgcacc gttggatatg cgcatggatc
    22981 agtcagctcc tttatccgct tatcacgtaa ttaatgaata ttcctaccat gaattagtaa
    23041 agattttctt ccgctatggc gaggagaaat tttccaaaca aattgctcgt gaaatcgaac
    23101 gagtacgaaa aacaaaacca atccaaacaa ctggtgagct tgtcgaaatc attaaacaag
    23161 tcatcccagc tcccgcaaga agaaaaggcg ggcatcctgc aaaaagaatt tttcaagctg
    23221 tgcggattgc agtaaatgac gaactcggtg ccgaagaggc ctctttagaa caggcaatcc
    23281 ggttattgaa agtcaatgga agaatcagcg taatcacatt ccattcattg gaagacagaa
    23341 ttgtcaaaag tatgtttaaa gaatatagta cggtacaaga tctaccaccg ggacttcctg
    23401 tcatacctga cgaattccaa ccagaactga aagtcattac tcgtaaacca atccttcctt
    23461 cagaggaaga gctaacagaa aacaatcgat cacgcagtgc aaagttacgt attgcagaaa
    23521 aaataagaat gaaagaggag tagaatgatg gcagaattaa agaaaatgca agattacgaa
    23581 tatgatttac ctgaattggt ggatcagccg gaacaacaaa tgcctgattc ttcttcgaat
    23641 caagaaatca ttcttccaca atcaccggca aaacgattaa agcagatttc tgctttggaa
    23701 aaaattgctg tggcaagcgt ggcgattgct attgttgctt tatgtgtttt aacagttatg
    23761 ctgcgcacaa atatcagcgg agctgaaaaa gaaatcacgt caatacaaac agaaatcaat
    23821 gataaaaagc aagaaaaaac gagtttagaa caagaaaaaa acgaactttc tcgtactgaa
    23881 cggattaaac aaatcgcaga agaaaaagga ttgtctatta atgacgacaa tttaaggaaa
    23941 gtgaagtaaa tgagtcttaa aaataaaatt cggcggttta tggaaaagaa aaatctaaat
    24001 ccgatgaaca atcgcaaaaa agtaggtatc attttatttg ctacgagtat tggattgttc
    24061 tttttatttg ccgtccgttt ctcgtatatc gttattggag gtcatgttgc tggtacctca
    24121 ttagctgaaa aaacaaaaca gttgtaccaa ggaagtgaag tagtaaaagc aaaaagagga
    24181 acgatttatg atcgtaacgg tgtggcgctc gcagaagatg cctcctctta ttcaattaaa
    24241 gcgatactct caaaaacata tacctctggg gataaaaaac tgtatgttga agaaaagaat
    24301 tttgataaaa tagcagaaat cctgcataag aatctttcta tagacaaaaa agatgcactg
    24361 aatattttag aggacggcgc aaaaaaagag ctgtatcaag ttgaatttgg tagctatggg
    24421 aaaaatatct cccaagaaac caagcaaaac atcgaagcgg acatgaaaaa agaaggtgtc
    24481 gctggtcttt attttgtcga ccatcaggct cggatgtatc caaatggcgt cttttcttct
    24541 cattttattg gatacgcagt tccagacaaa gatgagaatg gcttagttgg taaactaggt
    24601 ttggagtctg cttacaatga tatcttgagc ggtaaagatg gtaagattat ttatcaaaaa
    24661 gataattttc aaaatcctct gccaggtaca gtggcagaag aagaaaaagc tgtcgacgga
    24721 caagatatct acacgacttt agacagcaga ctacaaagtt atttagaaac actgatggat
    24781 caagtaaatg aagaatatca gccagaagaa ttgacagcag tcctgatgaa agcaaaaacc
    24841 ggagaaatct tggcaatggg acaacgacct actttcaatc ctgaaacaat ggaaggattg
    24901 actggaaaag atgctatctg gcgtaatttc ttggtacaag atagttatga accaggatca
    24961 accatgaaag ttttcactac tgcagctgct atcgaagaag gagaattcaa cgagaatgaa
    25021 accttccaat ctggaaaaat ccaagtagaa gatgctacga tcaatgacca tgactttggt
    25081 gaaaaaggtg tgctgaccat gcgtcaagct ctttcatggt caagtaacgt cgggatggtc
    25141 atactggaac agcgattagg cggacgatgg tataactatt tacaaaaact gggatttgga
    25201 caaagtactc attctggatt agatgatgaa gtaaacggtg ctttacctac attaaatatt
    25261 gtcgaccgag caatgagtgc ttatggacaa gcggtaggtg tgacgaattt ccaaatgatg
    25321 aaagctttca cttctattgc gaataacggt acaatgattc aacctcgata tatcagcaaa
    25381 gtggtggatc cccaaactgg agaagagcga acaactcaaa cagaagttct agggcaacct
    25441 ttttcaaaag aaacaacaga aaaagtccgt gaatatatga gagatgttgt cgaaagcgaa
    25501 aactacggta gtgcttatgg tgtatatagt gtacctggat acaatgtatc ggcaaaaaac
    25561 ggaacagccc aaatcgcttc tgatacaggg ggatatcaaa caggagatac agcctacctg
    25621 tattcgattg tggagatggt tccttctgaa gatccagact atgtcttgta tctaacaatg
    25681 aaacacccta agacatatga tcgaatggca ttggctaaaa tcgcaaatcc attgatgaaa
    25741 cgggcaatgg attttaaaga aactgaagaa gattcagata cagaaacaaa aacagaaaaa
    25801 gtatccgtag ccgattatcg taatcttgaa gcggacgttg cagcagctga tgctcaaaaa
    25861 agtggactgc aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca atctactgcc
    25921 aacggtgatc agttgatctc tggagaaaaa ctgatcctat atactggtgg tgataagctg
    25981 atgcctgacg taacaggatg gtcaaaagca gatatcatga agctggggaa aatcctcggt
    26041 attgaagtta gttttgatgg agatggttat tgtgtcaaac aagaacttgc tccatatgaa
    26101 aaaatcactg aagacaaact tagttttact ttagaagaat aagaagagaa ataggagaga
    26161 aaagagcatg gaatggacac aagcattgat tcccattgtc agcagttgtg caatgacaat
    26221 tgccgcaatg ccgctattta ttggttattt tcaaatgaaa aagcaaggtc aggcgattcg
    26281 tgaggaggga ccaaagtggc acaatagcaa agccggaact cctacaatgg gcggcttagt
    26341 gtttctgatc ggctctattt tgacaggaat ttgggttgga gcttggcaaa aacagctaac
    26401 tcctactctt ttcatcttgc tttttgtatt agcgttatac ggagtaatcg gctttcttga
    26461 tgattttata aaaatattca agaaaagaaa catgggactg aactcgaagc agaaattgat
    26521 tggacagatt ctagggggaa tcatctttta tcttgtttac cgttctgaag gttatccagg
    26581 tgtattgaat ttcttcggac tagatctacc tttaggaatc atttatggta ttttcgctct
    26641 cttctggctt gttggatttt caaatgcagt aaatctgaca gatggcatcg atggattagt
    26701 tgctggctta ggaagtatct cttttgcagc ttacggtcta attgcttggc atcagcatca
    26761 gtatgatgta ttaatcattt gcttaagtgt tttaggaggt ttactgggtt tcttcgtcta
    26821 caacagaaaa cctgccaaga tttttatggg agatgtcggt tcattagcgt tgggcggact
    26881 tttagcggca atttctataa tgctaaacca agaatggaca ttgcttttga ttggtttgat
    26941 ctatgttatg gaaacagcaa gtgtgatgct tcaagttact tcctttaagc ttacaggaaa
    27001 acgaattttt aaaatgtctc caattcatca tcactttgaa atgtgcggat ggtcggagtg
    27061 gaagattgat acagtttttt ggttgatcag cattgtgact tcgttgatca cattatggat
    27121 cgtttggtag gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat aagaaagtgc
    27181 ttgtcttagg actcgcaaaa agcggagtaa gtgcggccaa attattacat gaattgggcg
    27241 cacttgtcac cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca caagagctgc
    27301 tttctttagg gataaaagta ataacaggta gtcatccaat cgaattgttg gatgaagaat
    27361 tttcattgat ggtcaaaaat ccagggattc cctacagtca tcctttagta gcaaaagccc
    27421 aagaaatggg tattccggta attacggaag ttgagctagc ttacgaagta gcggaatgcc
    27481 caattattgg gattacagga acgaacggaa aaacaacgac aacaacgatg acaggactac
    27541 tactgaatgc aggagcagat caaggaattg cacgtttagc gggtaatatt gggtatccag
    27601 caagtggtgt agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa ttgtctagct
    27661 tccaactcat gggaatcaca gatttccgcc ctcatatagc ggtgattacc aatatctatg
    27721 aagcacatat tgattatcat ggtacaagaa aagaatatgt caaagctaaa tggaaccttc
    27781 aaaaaaacat gacagaaaaa gattatctga tccttaactg gaatcagtca gaactacaag
    27841 aattagcgca aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa gtgttggaag
    27901 acggtgtgta tgctgacgat tactcaatat actataaaaa agaaaaaata atggaaatat
    27961 ctgaacttgg cgtaccaggt aagcataatg tagaaaatgc actagctgct atttctgtag
    28021 caaaattgta tggtatatca aatgaagcta tacgagaaac gttgcatttt ttccatggcg
    28081 taccacatcg tacgcaatat gtaggagaaa tccaaggcag aaaattttat aatgattcaa
    28141 aagcaaccaa tattttagcg actaaaatgg ctctaagtgg atttgaaacg tctaaggtcg
    28201 tgttattggc tggcggatta gacagaggaa atacatttga tgaattaatt ccttcattaa
    28261 aaggaatcaa agcaatggtc gtatttggcc aaacaaaaga aaagttgatg gatgctggaa
    28321 aaaaagcagg catcgagaca atagtaacag ccgattctgt agaacaagca gttcctttag
    28381 cattagaaaa cagtacagat ggtgatgtag ttttattatc accagcgaat gccagctggg
    28441 atcaataccc aaattttgaa actagaggaa acagattcat ggaagctgtg aaccgattaa
    28501 aatagaaaaa gtagaagcga gcgataaaaa atgaaaattt tggttacagg cggaggaaca
    28561 gggggacata tttatccagc tcttgccttt gtcaattatg taaagactca agaaccgaat
    28621 gctgaattca tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt ccctgataca
    28681 ggaatgcctt tccatacctt ggagattcaa ggatttaaac gaaaaatttc catgcataac
    28741 atcaagacgg tccgattgtt tcttaagagt atccgtcaag caaaaaaaat cctcagtgat
    28801 tttcaaccag atatcgttat cggtactgga ggatatgttt ccggagcggt agtgtacgct
    28861 gcagccaaaa tgggtattcc aacagttatc catgaacaaa acagtgtacc aggtgttacc
    28921 aataaatttt taagccgata tgtggatcgg atcgctcttt catttaaaga tgcggcttcc
    28981 ttttttcctg aaaacaaagc agttcttgta ggtaatcctc gagcacaaga agtagcagat
    29041 actaaaaaat cagatatttt gaaaacattt gatttggatc cagaaaagaa aacagtcttg
    29101 atttttggcg gtagtcaagg agcacttaaa atcaatcaag cagtatcagt ttttttaagt
    29161 gaatatattc cttcttctta ccaagttttg tatgcttcag gagatagata ttatgaagag
    29221 atcctaaata aaataaatcc tgaaatacaa aatattagtg tccagcctta tatcaaaaat
    29281 atggcagagg tcatggcttc cagtgatctg ctggtggggc gtgctggtgc aacttcgatc
    29341 gcagaattca cagctctggg gttaccggca gtgttggttc ccagcccata tgttacgaat
    29401 gatcatcaga ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa aatgattgca
    29461 gataatgaac taacaggtga atcactaagt cagacagtca acgaaatcat gggtgatgaa
    29521 gaattgcaaa agcaaatgtg ccgagcttct aaagaacaag gaatccctga tgcttcaaaa
    29581 cgtctttatg acttagtaaa acaaataatc aaaaaataaa ggcggtggtc ttagtgagta
    29641 agaaaaaaga accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat ttgactcctt
    29701 ggcaaaaggc taaccgaaaa tatctggcag aacaagagat gaaaaaccaa gagccaaaaa
    29761 aagaagacat aacattagat acaccccttt cagattctga agaagaaaaa acggttcaag
    29821 aggaacagca agaagaaagc cagtccgaga aaaaaatcga attttttgaa gagatcaagc
    29881 aaaagaaaaa cggtggacca tataacggtt cttttttgaa ccggctgccc aatttaaaaa
    29941 gtcaacggca caaagtacta tttcgtcggt tagctttgat catcactgta ttgggtatac
    30001 cgctgatttt tttgatctat tatgtttctc cttatagcaa attacaagca gttgcagtat
    30061 caggcaataa gacagtcaac agccaagaaa tcatctctga tacaaaattg tcattaggag
    30121 aaaatgtttg gggacagtat tttcatcgat ctacttatat cgatcgcctt aaaaaagcac
    30181 agccaagaat cgaaacagca aatatacatt tcaaagggat gaatgaattc gatttggatg
    30241 taacagaata taaagagatt gctttgatcg caaaaaacaa tcaatactac ccagtgattg
    30301 aaaatggaac agtattagat gaaaaagtag caaatcctac aaaaaatctc cctatcttgg
    30361 aagagttcaa agactctgca aaaatcaaag aattaacaaa gcaatacaat cagttatcat
    30421 cagaattaca aaaagcaatt tctgaaatca agtacacgcc aagagcaagc aataaaaatc
    30481 tgatccaatt gaatatgaat gacggcaatc aagttattgt aaatatcaat aatcttgcga
    30541 accaaatgaa atattattcg caagtagcca aagatatgga tgaaaaaggt gtgatcgaca
    30601 tggaagttgg tatcttctcg catccttacg gtagcactca agaaacggaa gaatcgggcc
    30661 aatcggaaga aacaactgaa agtgaagaaa atgcggatca aacaaacgaa tcaagtgaag
    30721 aggcagaaac gcaaaaagca caaacacctg gatcgtctag cacaaacgga tcatctgatt
    30781 caaatgattc aagtgaaaca agtcaaatag aagataatcc aagtacaact ccgtcaagtt
    30841 taaatgaaaa ttgataaaac aactttaaaa agtaagaatg tactttctaa aaaccagtaa
    30901 tttatggtag aattggaagc agtgaatata tctattaata agtatatttt aaaacggacg
    30961 ctagttgttc gcttgtatat ggaatagtaa gtaggaggga atcccattca tggcaaaaac
    31021 aggaatgtat gtaggccttg atattggaac gacatctgtt aaagttgtcg tggctgaata
    31081 tatcgacagc caaatgaata ttattggagt aggaaacgca aaatcagaag ggattaaccg
    31141 aggcattatc gttgatatcg ataaaacggt ccaagcgata caacgagcag tacgacaagc
    31201 agaagaaaaa gcaggtatcc agatcaaagg cgtaagtgtc ggtttgccag caaacatgct
    31261 tgaagtagaa aattgccagg gaatgattgc agttaacgga gattcaaagg aaatcaccga
    31321 tgaagatgtc agaaatgtgg cttcagctgc ccttgttcgt tcaatacctc ctgaacgtca
    31381 aatcgtgtca atattgccac aagattttac tgtcgatggg ttcgagggaa tcaaagatcc
    31441 tcgtggaatg atcggcgtaa gattagaaat gtacggtctg ttgtttacag gacctaaaac
    31501 gatcatccat aacatacgta aatgcgtgga aaatgccgga ttaatcgtca acgaaatggt
    31561 gattactcct ttagcgctga ctgaatcaat tctttctgac ggagaaaaag attttggtac
    31621 gatcgtcatt gatatgggtg gcggccaaac aacaacggct gttatgcatg ataagcaatt
    31681 gaagtttacc aatcttgacc aagaaggtgg cgaatttgtc actaaagata tctctattgt
    31741 attaaatacg tcatttaaca atgcagaagc gttgaaaata aactacgggg atgcataccc
    31801 agaacggaca tcaccagatg aagaatttcc agtagatgtg attggtcagt cagaaccagt
    31861 gaaagtcgat gaacgctact tgtcagaaat tatttcagct cgaatggaac agattttcaa
    31921 caaagcaaaa gatgcattgg accaaatcga agcattagag ttgccaggtg gcgtcgtatt
    31981 gacaggcgga gctgctagtc ttcctggtgt tgtggacttg gcacaagaaa tcttcggcgt
    32041 aaatgtaaaa ttgtatgttc caaatcacat ggggctgaga aacccggtat ttacaaatgt
    32101 aataagtatc gtggattatt cagcaaactt gagtgaagtg tatcaattgg caaaaattgc
    32161 tgttacgggt gaaacctcag cagctcgtca aatggtagtt gaacaagaaa caacaaacac
    32221 atatgagagc tatgaagcac ctgaagaaac aatctacgat gagccggaac caaaagagtc
    32281 cggagaaaat gtaaaaaata aaatcaaggg tttctttaca aatatttttg attaattcat
    32341 cctgggagga ataagatatg gaattctcaa ttgataataa tatcaatgac ggcgcagtaa
    32401 tcaaagtcat cggagtcggt ggtggcggtg gaaatgctgt aaaccgaatg attgaagaaa
    32461 acgttaaagg tgttgaattc attacagcca acacagacgt gcaagcatta aaaaattcaa
    32521 aagctgaaac agttatccaa ttaggcccca aatatactcg tggtttaggt gctggttctc
    32581 agccagaagt tggacaaaaa gcggcagaag aaagtgaaca atccttacgc gaagctttag
    32641 acggtgcaga tatgattttc atcactgcag gaatgggtgg aggaaccggt acaggtgctg
    32701 caccaatcgt tgcaggtatc gcgaaagaat tgggcgcatt gactgttggt gtcgtgacac
    32761 gtccattcac ttttgaagga ccgaaacgtg gccgttttgc tgcagaaggt atcgctcgtt
    32821 tgaaagaaaa tgtggataca ttattaatta tctcaaataa ccgtttatta gaagttgtag
    32881 ataaaaagac accgatgctt gaagctttcc gtgaagcaga taatgtttta cgacaaggtg
    32941 tacaaggtat ctctgatttg atcacagcgc caggttacgt taaccttgac tttgctgacg
    33001 tgaaaacagt gatggaaaac caaggtacag cattgatggg gatcggtgtg gctagtggag
    33061 aagatcgcgt agtagaagca acgaaaaaag caatctcctc tccattgctt gaaacatcta
    33121 ttgatggagc tgaacaagta cttcttaaca tcacaggcgg tttggatatg acattgttcg
    33181 aagcacaaga tgcttcagat atcgtagcaa atgcagcaac tggcgatgta aatatcattc
    33241 taggtacatc tatcaatgaa gaaatgggcg atgaaatccg tgtgacggtc atcgcaacag
    33301 gtatcgatga atcaaaaaag gaacgcaagt ctagccgacc agctagacaa gcacaaatgc
    33361 aatcacctgc tcaaaagact gtattagata tggatcaagc taaaccaact tcttcagaag
    33421 aagaaaacag ttttggcgat tgggatatca gacgtgaaca aaatgttcgt cctagagttg
    33481 atgattccaa ttttgacaat atcgaaaaaa aagaatttga tacattcaac cgcgaagaaa
    33541 caaaatctca tggcgatgat gaattaaata cgccaccgtt tttccgtcgt aaaaaataaa
    33601 ggagaggaag agcacatgat tgctgacaac ttgcagaaaa tcaagcaaga aattcaggat
    33661 tcgtgtgctc ttgttcatcg tgatccgaaa gaagttacat tagtagctgt aacaaaatct
    33721 gttgaatccg ctcaagctga agaattggtt agagctggcg ttatgaattg tgcagaaaac
    33781 cgagtagata aattattaga aaagaaacaa gatttatttg attttcctga aataaagtgg
    33841 catttaattg gtaatttaca gcgcagaaag gtaaaattga ttgtaaatga aattgattac
    33901 tttcatgcgt tggacagttt aagattagca gaagaaatcc agaagcggtt agagaaagaa
    33961 ttatcctgtt ttattgaagt aaatgtttct ggtgaggaat caaaacatgg aatttcacca
    34021 gatgagcttt taccgtttgt ggaatcttta gctgcctgca caaaaatcaa ggttgttggg
    34081 ttgatgacta tggcgcctaa agaagcttct gatgagcaaa tccataaagt gtttggtacg
    34141 ctgaaagaat tgcgtgatct tgttcaagca aaaggttatg cccatgcacc atgtagtgaa
    34201 ttaagtatgg ggatgagtca agactttcaa attgcgattg aagaaggtgc aacatttatc
    34261 cgtgtaggaa cagcgctatt caaagacgaa taaaggagga acaaaaatgc cgcttttaaa
    34321 cagaggagca attgctaatt ttttcggatt agctgatgag gaagagtacg aataccaaga
    34381 ttatcctgaa acaacaaaaa aggctgtaca gccaactaaa aaaaatacaa cacagaatca
    34441 atcaactcat tctgtatctg ctgcgaatac tcatgaaaaa caaaaaacgg cgacttcacg
    34501 tcctgtaacg catgaaagta ttcagccggc tgaaaagaaa aaagtaattg aactacatca
    34561 atcacctaca aaaactgtag cgaaagaaag aacagcttca agaacctcta cacaacctaa
    34621 agcaaatctt gctggaaaaa tcactgtaat ggaaccaaga gcttattctg aagctatggc
    34681 gattgctaaa cgaatcattg ctggagaagc aaccttagtg aattttcatt tagtagaaga
    34741 aaaacaagcg agaagaatcg ttgattttct aacaggaaca gtttatgcac tagatggaga
    34801 tatcaaacgt gttggagatg agatttttct ttgtacaccg actgggatgg agattgacag
    34861 ttcaaccgct caatcttttg cagatgcgaa tttgtttgat ctgtaagagg aggaactacg
    34921 atttatacta ttgcactatt acttcattta cttcaacggg cagcctattt ctatagcatt
    34981 atcttgatgg tatatgcatt gctctcttgg tttccaggcg ggtatcaatc agggctagga
    35041 cgattattag ctaagatctg cgaaccgtat atcagtttgt tcgatcgtct gcctttgagg
    35101 attggaccct tggatttcac aattttaggg gcaatcctga tcttgaactt agccgtacaa
    35161 ggtttagcac agatcgttat atggataact gtccatttag tttattgatt gaagcaacaa
    35221 aatctttatt ctcagaatag ataggagggg aaaattttgg atgcaaatgt ataccagcac
    35281 tttcgggcag atgaacgtac atttatcgat tcggtaggcg attggattga acaggttcaa
    35341 ggacaatatg caccctatct gaccgaattt cttgacccaa gacaatccta tattttagaa
    35401 acactgattc gacaaagcag tgatttgcgt ttcatgtttt atggcggata tgaacaggca
    35461 gaaagaaaaa gatgtttgat tttccccgat tattacgaac ctgtagagga agattttgaa
    35521 acagcattgt atgaaatcca ttatcctagc aaatttgcca gcctaagtca tgggaaaatc
    35581 cttggaactt tagtgggaac ggggatcaaa cgctcttact ttggcgatat catctcggac
    35641 ggtgatcgct ggcaagtatt tattgcaaat gaggtggatc attttgttgc tgcacaagta
    35701 acaaagattg gaaaggttgc tgtacgcttg gaaccaatca aatacacaga aatgattatg
    35761 ccgaaagata gctggacaca agaaagagga acagttagtt ccatgcgact agacagtgtg
    35821 atctctgaga tttataatat ttccagacag cggtccaagc agctgatcga agctggtaaa
    35881 gtaaaagtga actggacaga aaatacaaga cctgattttg tacttgaact attagatatt
    35941 atttcagtca gaggttatgg cagagtccag atccaagaac ttgaggggaa gacgaaaaaa
    36001 gaaaagtaca gggtactgtt cggtgtatta cgtaaataaa ataaaaaggt ggtttactca
    36061 tggctttaac tccattagat atccaaaata agacattccc gacaaaaatg cggggatata
    36121 atcaagacga agtagatgat ttcttagatt tagttgttcg tgactatgag gaattgactc
    36181 aaagaaatcg agaattagaa aaagcagtca aacattctga agaaaaactt gagtacttca
    36241 atgaattaaa agatgctttg aaccaatcaa tcatcgtagc tcaagatacg gctgataaag
    36301 taaaaacaag tgcaagcaag gaatccgaag tgatcgttac ttctgcacaa aacaaagcag
    36361 atgagttagt tgcgaacgct gaaaaacgtg cacatcaatt aacaactgat gcagaagaaa
    36421 aagcaagaaa aatcttgaca gatgcaacag aaaaagcaag acagcttgcg acagaaactg
    36481 aagatcttaa gaaaaaaaca cgagtattcc atcaacgtat cagcttgatg cttgagtctc
    36541 aattagaaca agtgaaaagt ccggaatggg atgaaatttt acaaccattc tccagctacg
    36601 tgactgacag ccatgaagtg atcaaagaag ttttagcaaa acagcttgac aatgaaaatg
    36661 atactgatgt aaactcagac acaggagtca tagaagcacc tatgacggca tttgatacac
    36721 aggctattga tatctcgatc gtgcctggtg aaaatgaaaa cgagtagaac agaaaagcac
    36781 tttgttattc ttaaagcgag ccgatgagag tggaagttcg gtaagacact gcatggtgaa
    36841 agatcctcta cttgagacct gcttttccga aattagagta agaagcagcg gctgacctcg
    36901 ttattggtca cagagagttt gcactagata gatgcaacta aatttgggtg gtaccacggc
    36961 atgttcgtcc cttgcggctg aacatgcttt tttgtgtccg gaaaacaaat aaaggagtgt
    37021 atggttgaat gaaaatgaaa gaaaccttgc aattaggaaa aacaaaattc cctatgcgtg
    37081 gtaatttgcc aaaccgagaa ggcgagtggc aaaaagaatg ggaagaaaat cgaatctacc
    37141 aaaaaagaca agagttgaac gaaggaaaac ctagctttgt tcttcatgat ggacctccat
    37201 atgccaatgg taatatccat ttaggacatt cattgaataa aatcagtaaa gacattatca
    37261 ttcgttcaaa atcaatgtct ggctatcgtg caccatatgt tccaggatgg gatacacatg
    37321 gattgccgat tgaacaagta ttggctaata aaggcgtgaa acgtaaagaa atgtccatgg
    37381 cagaatactt ggaaaaatgt cgtgaatatg cactttccca agtagataag caacgagcag
    37441 atttcaaacg cctaggagtt gcaggagatt gggaacatcc ttatgttaca ttagatcctt
    37501 cttatgaagc tgcacaagta cgcgtatttg gtaaaatggc tgaaaaaggc tatatttaca
    37561 aaggattgaa accaatttac tggtctccgt caagtgagtc ttctttagcg gaagctgaaa
    37621 ttgaatataa agatgtaaaa tctccatcta tttttgtagc tttcaatgtc aaagacggca
    37681 aagatattct tgatgaagat acagcttttg tcatttggac gacaacacca tggacattgc
    37741 cagcaaacca aggtattgct gtaaatccaa aatttacgta tgtattggtg gaagccgatg
    37801 gccgtaagtt tgtcgtagcg aaagatttgc tagaaacagt ccaatcagaa atcggttggg
    37861 aaaatgtaac agttctaaaa gaatttgctg gacaagaaat ggaatatatg actgcaagtc
    37921 atccattcta tgaacgtgaa tcacttgtga ttttaggcga tcatgttact ttagacgctg
    37981 gtactggatt agtccatact gctccaggac atggggaaga tgactatatc gctggtagca
    38041 aatataaact atctgtagtg tcacctgtag actcaaaagg attgtttaca gaagaagcac
    38101 ctggatttga aggtatcttt tatgacaaag caaatcctat gatcactgat ctattaaaag
    38161 aaaaaggagc tttgctgaag ctagatttct ttactcatag ttatcctcac gattggcgga
    38221 cgaaaaagcc tgtgatctat cgggcaactc cacaatggtt tgcatctatc gataaattcc
    38281 gtcagaatat tttggatgaa gtagaaaaag tagagtgggt tatcccatgg ggtaaaacac
    38341 gtttgtataa tatgatccgt gaccgcggcg attgggtaat ttcccgtcag cgtgcatggg
    38401 gagtaccact tccgattttc tatgctgaaa atggagaagc aatcatcaca ccagaaacaa
    38461 tcgaacatgt ggcaaaatta tttgctgaac atggatcaat tatctggttt gaaaaagaag
    38521 cgaaagaact tcttcctgaa ggatttaccc acccaggttc tcctaatggc gaatttacaa
    38581 aagaaaaaga tatcatggat gtctggtttg attctggttc ttctcatgaa gctgttttac
    38641 gtcagcgacc agaattatca ttcccagcag atatgtattt agaaggatca gaccaatatc
    38701 gtggctggtt caactcaagt atcacgacta gtgtggctat caatggtgta gcaccataca
    38761 aatcagttat ttctcaagga tttgctttag atggtgaagg ccgtaagatg agtaaatctt
    38821 taggaaacat cattgcacca gaaaaagtga tcaaacaatt tggtgcagat atccttcgct
    38881 tatgggtatc tagtgtagat tatgaagcag atgttcgtgt ttctatggat atcttaagtc
    38941 aagtggcaga agtatatcgt aaaatcagaa atacgatgcg tttcttatta gcgaatacag
    39001 aagatttcga tcctaaaaaa gatactgttt cgtacaatga tttacgttct gtagacaaat
    39061 atatgaccgt tcgattgaac caagtaatca aagaaatccg agaagaaggg tacgacaaat
    39121 ataacttcat gcatatttac cgtacagtta tgaacttctt gacggttgat ctatcgtcat
    39181 tctacctgga ttttgctaaa gatgtagttt atattgaagc agaagatgat tatcaacgtc
    39241 gttgcatgca aactgttttc tatcaaacag ctgtagcttt gacaaaatta ttgactccta
    39301 tcattccgca tacagcagaa gaaatctggt cattcctgaa agaagaagag gagtacgttc
    39361 agttaagcga gtttcctggt tatgaagagt ttgcaaacca agaagaattg atggatacct
    39421 ggactgcttt catggacttc cgtgataatg tcttaaaagc attggaagaa gcaagaaaca
    39481 gcaaattgat tggtaaatct ttggaagcaa aactaactgt ttatccaaaa caacaagttc
    39541 gtgaaatgtt aaaagctttg gatgcagata ttgcacaatt attgatcgtt tcaccagact
    39601 actttgaagt tatggcagca gatgaacaag tgccagataa cgctatggta tttgatgatg
    39661 ttgctattac agtggaaaaa gcagacgggg aaacttgcga tcgctgtcgc caagttcgta
    39721 aagatgtagg agtagatgaa aaacttcctc atctatgcgg tcgttgtgca aaaatcgttg
    39781 aagacttcta tcctgaagct gtagctgaag gattcgaaga aaagtaaaag ggaaaagggg
    39841 gtgtgacatg ttctttgtca cacccccttt tgttatcaat cgtttaattt tccacgttca
    39901 cgataccagt tatctggttg ccagatccat tcgaatccgt ctttttcaag tagttcgaaa
    39961 gctgctttag gtcccattga tccagctttg tagttgggaa aatccggttg tgtttggtcc
    40021 caagcttttc gaatgacatc cacgatatgc caagattgag cgacttcttc ccaatgagag
    40081 aagttcgttc catcaccatt caacgcatct aatagtaatt tttcgtaagc ttctgggcta
    40141 ttttcaacca tttctgcact atttctaaat tccaatttga ttggttccgt ttcaaatcct
    40201 tgccctactt ctttcccatt caaggataaa gaaaatcctt cagttggctg aatatagatc
    40261 gttagaacat taggtggaag tgtcgtatcg tcacatggtt catcgacaga tgttttgaat
    40321 acattaacag gaacttgttt gaatacgatg ttgatccgag tccctttttc agtcaaacgt
    40381 ttgcctgtac gtacatagaa aggaacacct gaccaacgga agttatcgat agtgaatttg
    40441 cctgcgacga atgtttcagt ctctgattga gcatccactt tgtcttcttc gcgatagccc
    40501 ttgaattgta caccatccaa ttcaccttga gcatattgcc cgcgaacgaa gttttctaac
    40561 acttcttctt cgttataaat gcggatagct ttcaatgctt tgatcttttc tgtacggatt
    40621 tctttttcgg aaaatgctac tggcggttcc attgcaagta atgctacaac ctgtaaaata
    40681 tgattttgca ccatgtcttt aagtgcgcca ctatgatcgt agtagccccc gcgatcttct
    40741 acaccgaggt tttcgccaaa agtaatttgg atattgtcga tatagcgatt gttccattga
    40801 gcctcaaaaa tattattggc aaaacggata gctgagatgt tttggatcat ttcttttcct
    40861 aaataatgat caatcctgaa aatatcttgt tctgggaata cttctctgat ttgctggttc
    40921 aattcaaaag cagactggta atcagaaccg aacggttttt ctatgatcaa acgattgaag
    40981 ccattttttg tcataatatt ttgtgatttc aaatgagaaa cgatcgttcc aaaaaattga
    41041 ggtgccatag ccaaataata gacacggtta ccgtttaatt gatagtgtcc atctaatcgg
    41101 tcagacaatt cttttaatgt ctgataatgc gttgaatcat ttacatcatg ggattgatag
    41161 taaaaatggc ttgcaaaggt agttgcttct tcatctgaag gattcaatgc acggattgtt
    41221 tctttgacaa cttcacggta gtgctcatcg ctccagggac ggcgagcggt tccgattaca
    41281 gcaaaatgct cgcctaaatt tccttttctg taaaggcgga acaaagacgg gtacaattta
    41341 cgctgggcta ggtcacctgt accgccgaat atcgtaaaca atacattttt ttcattagac
    41401 atgtggatac actcctttat attttatcga caaaaatcgt gctggccgcc ttaaagctga
    41461 cggggatttt ctttctatca ttttggatag tgatcggtcc ttcataagct gcgatgtcaa
    41521 cgatctcata ttcttcgttg atattcaggt caatagtgac taagtaatcc aacaattctt
    41581 tttcatcaag cactcgtgcg atacgcactt ttgtaccgac aggataggac tccaatgttt
    41641 gtcggcgttc ttcatggaca acttcctctt ttttaggaat cgatcctcca tgaggacaat
    41701 gttcaggatg attcaaatag ttttctaaat gatctgcaag atgttccgaa gtcacatgtt
    41761 ccaatacttc agcatcatca tggacttcat tccagctata gtctaaatgt tcaactaaaa
    41821 aaacttccca taagcgatgt ttgcgaatca gactgctagc tttttgcagt ccgcttgcag
    41881 tcaattgtac accttgataa ggggaatgtt ctacaagctc ttctttcaca agtttagaaa
    41941 tcatttcact gacagatgca gctgagacat ctagactaga tacgatttgt ttattgttga
    42001 ctttcgttgt atctccacct agttctaaaa tgattttcaa ataatcttct cgatttggtg
    42061 tcatattcga tcatcctttc atggtaaaaa accatcatta gtaagtgtaa caaaaaaaac
    42121 gttaattgac tatttttcta aataaaaaac aaaggatttt tagacgtttt tacaacaaaa
    42181 aacgaaagat gatagagaag actcattctt tcgtttataa aggatatata tatacattgg
    42241 gagggatcta tttttcagat aacaacttat atgaagtgtc tgcagattcg tcataaagag
    42301 tttgttcggg ataagaaaag ataagataaa aaccatactg atcacgtacg actaaacgat
    42361 attcatctat ttctaccaca gatgtgccaa gagactgacc gttgatcaaa aacgtgtaat
    42421 cagatgtaat ctgtaattgt tgtttttgct ggctatcact aaagaaccat gtaccttgat
    42481 actttgagag tccttctgac ttttccgctt gttttttctt tttcaagtaa ataaaacttg
    42541 agagaacggt aatgagtgaa ccaagaaaat agatcatttt ttttatcacc aaatcactcc
    42601 tttatgagaa aataatcgca tagacatatt ctttcttctt tatttcattg taccgaataa
    42661 aagctaaaat ttctctaaga ctttcgactg gtttttttac tgtttttcta caaattatga
    42721 taggatacat agtaggaaga aaggtgacat aatttgaagt atcagcaagt ggaaatagct
    42781 ttttttattg accgcccgaa tcggtttata gcacattgta tgaaaaaaga cggagaaata
    42841 attattgcgc atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc aggcgctgaa
    42901 gtggctattg tatttgctcc tggacctaaa cggaaaaccg cctatgatct gattgctgta
    42961 aaaaagaagg gtgactggtt taatatcgat agtcaattgc ctaatcggct ggcaattgat
    43021 ggtattttag acggcacgat ccttttacca ggattaactg gtccatttac ggtatacaag
    43081 cgtgaagtaa cttatcttca ctccaaattt gatatttatt tagagacggt ggtcggacaa
    43141 aaagcatttg ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg tgcttttcct
    43201 gatgcgccca cactcagagg attgaaacat ataggagaat taacgtatgc cgcacaagat
    43261 ggttatgccg cttatgtctt gtttattgct cagttcgaac atcttcatct ggcaacgatc
    43321 catgaagaaa tgcaaccagc actagctgat atggttcgac atgcgcagca aagcggagtt
    43381 caaatcctcg cctataattg tcaagtatcg gcagatgagg taactgtcaa gcaggccatt
    43441 ccatttgatg tagatatccc ttttgaagat cctatacaat agaaagaagg taaaataatg
    43501 atcaacttag gtattatcgg aacaaattgg attactcatc aatttgtcca agcagcttta
    43561 agcacaaaaa aatatgaatt gacagcggta tattctagac atttggaaaa agcacaggaa
    43621 tttggttctc attatgaagg ggatatcgaa ttcgcaacag atttgaagac ttttttcaat
    43681 attactcata tggacaccat ttatattgct tctccgaata gtcttcactt tagtcaagct
    43741 aagcaagcaa ttttggcagg aaaaaatgtg atagttgaaa agccagcttt ttcgacgcca
    43801 aaagaaatgg atgaaatcat ccatttggca aatgaacaca atgtgtattt ttttgaagca
    43861 gcaagaaata tccatgaatc aggttttaaa aagattgccg attacttacc attaaaagac
    43921 gagataatcg gtgcaaattt cagctatatg aaatattctt cgcgctatga ccaagtatta
    43981 gatggagaag aaccgaatat cttctcgact catttctcag gtggtgcatt gatggattta
    44041 ggtgtttacc ttgtatatgc agctgttgct tggtttggaa tgccaaaaga agtccattat
    44101 ttcccaagaa agatttctac tggtgtagat ggtcttggat gggggatctt acgttatgag
    44161 aattttgatg tatcgatcca gccaggtaaa atcggtgatt cttatttacc gtcagagatt
    44221 tattttgact caggaacgct agtgatgaat ggagtcaatg caattgaaca agcacaattt
    44281 catgatcgcg cgcataaaga aatcattgat ttggcggtaa cagctgcaga aaatccaatg
    44341 gcagatgaag cagaggattt tgccaatgtc atggctcatc cgaaagatcc agcttgggga
    44401 ttgttgtatg aagagtggat cgagctagct agaaatgtga accaagtgat ttatgattta
    44461 agaaaaaatg gcggcatcaa attcgatgca gataatgagg aagattttta atgaacgaat
    44521 ttgtacaagt atttcccgaa aattggcaga aaaaatatga ggataaaggg tttatctcac
    44581 cttctcttat tcaacgagca gtttttcagc cactaagtaa taaacaaagt atagtagcgg
    44641 tttctcctac aggttcaggt aaaacactgg cttatctttg gcctctacta ctaaacgtgg
    44701 aaccaggtga agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta gctattcaag
    44761 tagcagatgt tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa tctttggtgg
    44821 gcggagccaa cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa atcttgattg
    44881 gtacaccagg aagaatattg gaattgatga aagcaaaaaa aataaaagca catcaagtga
    44941 aaacaatggt atttgatgaa gcagaccagc tatttgacgc tgggaacagc caaatcattg
    45001 atcaaattct tcatcaagct ccaacagagt atcagctagc ttttttttca gcgacagcag
    45061 atcgttccct tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca attgacgtga
    45121 cagcagagga cgattctcga aaaggactga gacattactt tttacgtgta ccgatacgaa
    45181 agaaagatga ctacttgaga cgtctcacgc atatagaaga ttttcatggt ctgattttct
    45241 ttaatcaact gactgaatta ggcgtaatgg aagaaaaatt gctgtataga ggtgtaccgg
    45301 taggaagtct ggcctctgat cagaacaagc tactccgaaa agcagcgcta gaacaattca
    45361 aaaagaaaat ggtcagagca cttttgacca cagatgtggc ggctagaggt ttagatatta
    45421 caggtcttcc ttatgtagtg aatgcagaag ttccgttaag tgaagaagct tatttgcatc
    45481 gttcgggaag aactggtcga atgggtaatg aaggaatagt aattacactc gtacaagata
    45541 ataatctaag agatttaaaa cggcttttac gtccatcaaa tataacactt gaagagattt
    45601 ttttatacgg aggtaagctg caaactgagc agccaaccaa agaagatgca gctcctgcag
    45661 taaataaaaa gtatgcgaaa actttttctg gaaaggagca gaacccagaa tataacaaag
    45721 aaaaaataag aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa ggagcaaggc
    45781 gcaaataagc gcctttttat gctatgatta tgaaaacggg aacaaaattg gttgccagag
    45841 gaggggttat gtgaaagaac gtcaagaaga gatcttgcag ctattgacag aaaaccaaca
    45901 aggattgaca gcaagtgatg tagctgagcg cttaactatc gatcgaagca atgcaagccg
    45961 atatttaagt gaactataca aggcccatca tattgtaaaa accgctggcc gaccagtcgt
    46021 ttactctttg ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact cttctacgca
    46081 agtgactttt gaaacactcg ttggagaaaa tgattcttta aaagtcagca tacagcaggc
    46141 aaaagctgcg attctttatc caccgcgggg gttgcatacg attatttttg gagaaactgg
    46201 tacagggaag tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt cagagatgct
    46261 ttctgcagat gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa atccgcaact
    46321 gctctttgga cacatctttg ggatcaaaaa aggggcttac acaggagcag ctcaagatag
    46381 tcccggtcta atagccaaag cagacggcgg tatattgttt ttagatgaga tccaccgttt
    46441 accgccggaa gggcaggaaa tgctgtttac tttcattgat aaaggtattt accgtccgct
    46501 tggagaaagt gcacaagtac atgaagcatc tgttcagatc attggggcga cgactgaatc
    46561 ttcagaaagt ttcttatcta cgttcaatcg acggattcct atggcaatca ccttgccgaa
    46621 tcttgcttca cgttcactgg atgagcgtta tgaaatcatc tctcttttta tcaaacaaga
    46681 agcgaatcgt ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg ccttcatgct
    46741 ttatgatgcg gaaggaaata tcggtcaagt gaaacgagac ttgaaactag tctgtgcaaa
    46801 atctttttta cattaccgta ctcatcatga gaaaaagcta atcattcgaa aagaagaact
    46861 ttcccttcaa gtacaaaaag gattactgaa aatcaaggaa ataccggaac gtctcgaccg
    46921 attcatggac agcaaaagtc agtatttgac atttgaacca ggatttgctg atgttgtctg
    46981 gtcacaagat ccagaacgaa acatgcaggt ctataacgat attgaggaaa aagtcacttc
    47041 tctttccgaa accggtgtgg agaacattga tctagagacc ctcatttcaa aagatgtgga
    47101 tgcttatttc cagacatatg tcaaagaatt gactaaaagt tcgattccca aagatctttt
    47161 gccagatgaa atctggcggt taaccaatca attgtatgat gtggctgaaa aacggttaga
    47221 ccgtatttat aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa gtacattgga
    47281 tcgtgtgaga gaaggtcata tgatcgtcca cccagatttg aataacgtac ggaaaaattt
    47341 taagagggaa ttccaggtag caatcgatct ttcaacgatc attgaagaag aagaacaagt
    47401 tgagattcct tttgatgaaa tcggctttat tagtatgttt ctttctatca atctaggaga
    47461 aagtgagcct gttaaagaaa acaaagtaga tgttctcata ttgatgcacg gcagacagac
    47521 tgcttcaagc atgttggaga tggcacaaga actattagga acaaaagtag ggaaagcttt
    47581 taatatgcca ctggttatgg aagttcaaac aatgtatgaa gaagtgttac aatatgtcaa
    47641 aaactatcaa gagcatttga cgaatggcct actcttgtta acagatatgg gatcattaaa
    47701 caccttcagt gatttgattt atgaagagac tggcattcgg acgaaagcta tcacgatgac
    47761 aagtaccatg attgtgatag aagcgatacg gatggcaagt gttggaagaa gcttagaaga
    47821 tatttatcaa aatatccagc tttcctttga atcgatcgta cgtgatcagt tcaaagcgga
    47881 aaagtctgaa actttacagc gtaaaaaagc cgtaatcgtg acatgcttta ctggtgaagg
    47941 agtagctgcc aaattatatc agcgtattat gccagtgatt aatcaaaata aggtagaact
    48001 gatccagatg cagtttttag aacgtgaatc attcaaaaaa cacatcgatg ccttattgga
    48061 agaatatgag atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa atatcccatt
    48121 tttttcagcc tatgacgttt ttgatgatga aaaactaaat attttaaagc gtatagctga
    48181 tgatgagatc ccgacagcta aaattgtcga ttcacttgct ggatcattaa gcgatgtctt
    48241 ttctattgat gaactgatcc gttcattgca aaagacagtt cagcagatcc agaccgattt
    48301 gtcgattatc gtggaacctg gagtagatac tggaatcgtt atccatttag ccttcatgtt
    48361 agacggattg atcaaacaag aaagccatcg gcaatttgag aaactgtcta ttttctcaaa
    48421 aatttatcgt ttggaaatgg atatcgttcg ggcaaacttg atggcactag aacgaaaata
    48481 tcaggtaaaa attccagaag atgatatcgc atttcttaca caaatgtttc ttgaaaataa
    48541 aataaaaaaa tgaacacact aatcatactg tttacacact aaacatctgt gtgcgcacta
    48601 taaaaaagag ctaaaaagca tgaaaacgct atttcagctc ttttttttat tggcatgctt
    48661 cttgcgttat ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca aaaaaaacaa
    48721 tcatgttagt ttgttcagca gggatgagta cgagtttatt ggtcacaaaa atgcaaaaag
    48781 cagcagaaga aaaagggatg gaagccgata tttttgcggt ctctgcctca gatgcagata
    48841 ataatttaga atctaagaat gtcgatgtat tattattagg tcctcaagta cgtttcatga
    48901 aagctcaatt tgaacaaaaa cttgcgccta aaggtattcc attagatgtc atcaatatgc
    48961 aagactatgg catgatgaac ggagaaaaag ttcttgcaca agcagaaaat ctaatgaaat
    49021 aaatcaagta tgcagttggg ggtataacaa cgtggaagat caacaaaatc tagaagccat
    49081 catggggtta atcatgtttg gcggtaatgc aaagagcgat gcaatggaag cgattgcggc
    49141 tgctaaaatc ggtgattttg agttagcgga taggaaaatc gctgatgcag aagaatcact
    49201 tatacaagcg catcatgcac aaacagacat gttgacacaa gaagcacaag gaaatcatat
    49261 gcaagtgaca ttattgactg ttcacagtca agatcactta atgacatcta ttgcgtttac
    49321 tgatttagca aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat aaatacaact
    49381 tcttttttga acggaagaag aaaaattttt gaacggattt tttattttgt tataaggaga
    49441 actctcttta tataaaaaac ttataggagg aagtaaaaaa tgaacggact atcaagttgg
    49501 atggagaaat acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca tttagttgct
    49561 ttgcgtgatg cttttatcgg cacactgccc gcaacaatgg cgggatcagt cgctgtaatg
    49621 atcaatgcaa ttattcgtga tttaccgcaa cagtttaaca gtacctatgc tgacagttta
    49681 gcagcagaca aaggaatctt tgccgtattg aatgtcatca tcggaatcaa tggttttgtc
    49741 tggaacggta cactagcaat cgccggattg atctttgcct tttcttgggg gtataatctt
    49801 gcccgagctt atggagtaaa tgatctagct ggggggatcg ttggtttagc taccttgatt
    49861 caaggtattt cctttggtcc agcattatca gcagcattag atgtgaatgt tccttcaaat
    49921 gtcgcagatg caatcaatag ttctgagatt ggtgcaacga ttgcagatgg cacactgtca
    49981 gttggattat ggggttggct aaaactagat catttgaatg gaaacgctta ttttacagtt
    50041 atgatcatgg gagctatttc agtcattatt ttttgtaaat tgatgcttgc caacattacg
    50101 atcaaaatgc ctgattcagt accaccagca gtatctaaag catttgcagc cattttacca
    50161 gcgacgatcg ctttatacgt cattgctatt atcaattata tcgtaggtcg tgtagctgat
    50221 gggcaactga tcattgattt ggtgcaaaaa tatattgcag agccattcct aggattatct
    50281 caagggttgg gagcagtatt gatcgtcaca gtatttgttc aagtattctg gttctttggt
    50341 attcatggac caaatgtatt ggcaccagtg ctagagggaa tctggggaca agcacaacta
    50401 atcaatattg atattttcca aaaaggttat aacggaaaaa ctggtacacc agccgtgtta
    50461 gatgcaatcg aagatggaaa agcttatatg tgggtccgcg gatcgttcga tgcttttgca
    50521 tggtttggcg gatcaggcgg aacgatcgtc ttactgatag ctatcttgat tttctctaaa
    50581 cgtgcagatt atctaacagt agctaaatta tccattgggc ctggtatctt caatatcaac
    50641 gaaccagtga tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat cccattcttg
    50701 attgctccag tagtagcaac agcaattgga tggacagcta cttatcttgg cctagtcgct
    50761 ccggtatcgc agcaagtcac atgggtagta ccgccgttct tattgtcatt cttagcaaca
    50821 ggagcggatt ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac tttcttgatt
    50881 tgggcaccat ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga acaacaataa
    50941 gatgaacgtg aggagcggga cgctttaagt ttcgttcctc ataatgaatt ttcgaggagg
    51001 aactgaaatg attacaattg ccagtgcaat gcaacaagat gaaatcaaag aattgttaga
    51061 aggatacaat gaagaaggaa tgacttttac atttaaagaa aaacaaggaa tcaagctgtt
    51121 ctttgaaaca agtgcagaag atccggaagt tgcggctaaa aaagcaaagg aactaatcaa
    51181 agcacagcct tggggaacag tcctttattt tcaatcaaca gctgtttaga aaatggttta
    51241 aaaagatagg atggttgaga tgataaaaaa cggtttattt ttatgtagta ttggttcttt
    51301 gatcgtgttg tatagtttga atccacattc gcaaaggtat gatttgtata gtatggtcac
    51361 aggtcttttt ctaacaggat taggaatcta tttcttcatg aaggggaaaa aaagagaaga
    51421 agcaaaggag aaaaataact aatggaaatc acgaaaaaat taaatattcc agcatcgttt
    51481 ttttacgata aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac cggcaaatca
    51541 gtaactagaa aacagctgaa aaatttggag tacgtcaaac aattctcgca aaataaccgg
    51601 gctcgcataa aaatcgaaga agtcattgaa aaccagtctt ataagtttcg tacatcgaca
    51661 acgaagaatg aattcgtcgt tcattatcaa atcaaaccat tggacgattc gacctgtgag
    51721 gttcattaca cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa tgatatgctt
    51781 ttaggaacaa tcttgatgta ttttaaacgg agacgtttca aaaaaatgct agatatgatc
    51841 gaagcaacgt attaaatcaa tgaaacatgg tactatttag ttattgaaaa atgaagtaag
    51901 ctagaaggaa tttttcatag gaagatagat ctttcctgcg aatcctcact agcttttttg
    51961 taggaagggg cagcaaaatg acaaacaaag taagagagct aggtgtatct tcgattgagg
    52021 aaatgtttga tttagccact tatgcattta atggggcaga tacacctgaa aggcgagaac
    52081 gttttcgtac acttgcggag aattcttgga attacggatt ttttgatgag gaggatcggc
    52141 taacaagtca agtcatggcg accccttttc cagtgaattt ttacggacaa gagtacctaa
    52201 tggccggcat tggctatgtt gcttcttatc cagaagcaag gggacaaggc gggatcaatc
    52261 agatcatgga aaagatcctt gaagattgcc gtgatcgtaa agtaagctta tcttatctgg
    52321 cgccattctc ctatcccttc tatcggcgct atggctatga acaatcattt gataaaatca
    52381 gttatcaact aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga aaaataaagc
    52441 gtatggattt tgaagatgca aaagctgcaa tcagaagaat ttatgaacag atgccggaaa
    52501 accaacgagc tggtctgaag cgcgaagact ggtggtatac gtataaattc aagcaaaaag
    52561 gcaattacca gtttgctttg cattttgatg ataatgagga agcaacggga tatatcgttt
    52621 atcagcttgc aacgtcttcc tttattattg cggagtgggg atttttggat catgatgctt
    52681 tttgcagcct tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca tttgaatatt
    52741 catgcggcta cggcggtaat gatcaaaatt atctattaga aaatccattt gcttctgtaa
    52801 tgatcacacc ttatatgatg gcgaggatcg tagatatttc tttgtttttg gaaaattatc
    52861 catttaaaaa gagacaagct gcatttgccc tacaaataga agaagatcat tatgcgaaat
    52921 ggaatgaagg aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc tcaaaagtag
    52981 aacaaacaaa actcccaatc gttcgaggaa gtatccaaca tatcacgcag ctattattag
    53041 ggtatcgtaa agtggaagag ttggtattcc aagaaagaat acaggtgaca aaagaatatg
    53101 aagaaacgtt aagctcaatc ttgccacagc aacagccaat tcttaatgac tatttttgat
    53161 aaaaaacggg tgatgacaat atttttgtca ccaccctttt gtttttcatt tggcatttga
    53221 aggaaaatgt taaagtaaat cctctttttt cgtaaatttg aattttttgt tttcttttgt
    53281 tttagggttt tcttttttgt ggtcttggat tgcttctttc gtaatctttt cccgattttt
    53341 tttagatgaa tctaagcgtt ctgcgtatac tttttctttt ggcttatcat tcattggaaa
    53401 ggacctccct acattatttc attctacctc tagtttatca tctgttaagg actagtggaa
    53461 acgttttgcc taacttataa gctgattttt ttagcagata agcttgcaag taaaaaacaa
    53521 acaagctaca attgatttat tgaaattgtt cctgtagctc agtaggatag agcaatcgcc
    53581 tcctaagcga taggtcgctg gttcaattcc agtcaggaac gtataaataa cccctgtatc
    53641 ctttgtggta caggggtttg ttttgtattt gtgccaaaaa tgtgccaaac tagttatcat
    53701 ttttatataa actatcgaat acagcattga ttttttcttt atcctgatct tcaagctctc
    53761 gaacaatatg aagataagta gacattgtag tttctaaact gctatgtcct aaccgttttg
    53821 atacgctaag tatattt
//
LOCUS       DAZUKZ010000007        50999 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000007 DAZUKZ010000000
VERSION     DAZUKZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.007"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..955
                     /locus_tag="ACG8EF_000399"
     CDS             110..955
                     /locus_tag="ACG8EF_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4945757.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1236..2456
                     /locus_tag="ACG8EF_000400"
     CDS             1236..2456
                     /locus_tag="ACG8EF_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS4945758.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2453..5173
                     /locus_tag="ACG8EF_000401"
     CDS             2453..5173
                     /locus_tag="ACG8EF_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4945759.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5157..6101
                     /locus_tag="ACG8EF_000402"
     CDS             5157..6101
                     /locus_tag="ACG8EF_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4945760.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6170..7180)
                     /locus_tag="ACG8EF_000403"
     CDS             complement(6170..7180)
                     /locus_tag="ACG8EF_000403"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4945761.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7360..7704)
                     /locus_tag="ACG8EF_000404"
     CDS             complement(7360..7704)
                     /locus_tag="ACG8EF_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS4945762.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7697..8131)
                     /locus_tag="ACG8EF_000405"
     CDS             complement(7697..8131)
                     /locus_tag="ACG8EF_000405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFS4945763.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8269..9006
                     /locus_tag="ACG8EF_000406"
     CDS             8269..9006
                     /locus_tag="ACG8EF_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4945764.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8999..10210
                     /locus_tag="ACG8EF_000407"
     CDS             8999..10210
                     /locus_tag="ACG8EF_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4945765.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10414..11196
                     /locus_tag="ACG8EF_000408"
     CDS             10414..11196
                     /locus_tag="ACG8EF_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFS4945766.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11266..11928
                     /gene="trmB"
                     /locus_tag="ACG8EF_000409"
     CDS             11266..11928
                     /gene="trmB"
                     /locus_tag="ACG8EF_000409"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFS4945767.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12026..13780)
                     /gene="ade"
                     /locus_tag="ACG8EF_000410"
     CDS             complement(12026..13780)
                     /gene="ade"
                     /locus_tag="ACG8EF_000410"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFS4945768.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13912..14841
                     /gene="comGA"
                     /locus_tag="ACG8EF_000411"
     CDS             13912..14841
                     /gene="comGA"
                     /locus_tag="ACG8EF_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFS4945769.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14798..15847
                     /gene="comGB"
                     /locus_tag="ACG8EF_000412"
     CDS             14798..15847
                     /gene="comGB"
                     /locus_tag="ACG8EF_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFS4945770.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15844..16122
                     /gene="comGC"
                     /locus_tag="ACG8EF_000413"
     CDS             15844..16122
                     /gene="comGC"
                     /locus_tag="ACG8EF_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFS4945771.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16097..16567
                     /gene="comGD"
                     /locus_tag="ACG8EF_000414"
     CDS             16097..16567
                     /gene="comGD"
                     /locus_tag="ACG8EF_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFS4945772.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16539..16886
                     /locus_tag="ACG8EF_000415"
     CDS             16539..16886
                     /locus_tag="ACG8EF_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945773.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            16849..17274
                     /gene="comGF"
                     /locus_tag="ACG8EF_000416"
     CDS             16849..17274
                     /gene="comGF"
                     /locus_tag="ACG8EF_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFS4945774.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            17246..17605
                     /gene="comGG"
                     /locus_tag="ACG8EF_000417"
     CDS             17246..17605
                     /gene="comGG"
                     /locus_tag="ACG8EF_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFS4945775.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17777..18784
                     /locus_tag="ACG8EF_000418"
     CDS             17777..18784
                     /locus_tag="ACG8EF_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4945776.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18809..19993
                     /locus_tag="ACG8EF_000419"
     CDS             18809..19993
                     /locus_tag="ACG8EF_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFS4945777.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20430..22013)
                     /gene="abc-f"
                     /locus_tag="ACG8EF_000420"
     CDS             complement(20430..22013)
                     /gene="abc-f"
                     /locus_tag="ACG8EF_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFS4945778.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22118..22246)
                     /locus_tag="ACG8EF_000421"
     CDS             complement(22118..22246)
                     /locus_tag="ACG8EF_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945779.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(22591..22734)
                     /locus_tag="ACG8EF_000422"
     CDS             complement(22591..22734)
                     /locus_tag="ACG8EF_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFS4945780.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22956..23435)
                     /locus_tag="ACG8EF_000423"
     CDS             complement(22956..23435)
                     /locus_tag="ACG8EF_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4945781.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23508..23792)
                     /locus_tag="ACG8EF_000424"
     CDS             complement(23508..23792)
                     /locus_tag="ACG8EF_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945782.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23870..24064)
                     /gene="ssrS"
                     /locus_tag="ACG8EF_000425"
     ncRNA           complement(23870..24064)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8EF_000425"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24136..25422)
                     /locus_tag="ACG8EF_000426"
     CDS             complement(24136..25422)
                     /locus_tag="ACG8EF_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFS4945783.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25560..26231
                     /locus_tag="ACG8EF_000427"
     CDS             25560..26231
                     /locus_tag="ACG8EF_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFS4945784.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26197..26685
                     /locus_tag="ACG8EF_000428"
     CDS             26197..26685
                     /locus_tag="ACG8EF_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFS4945785.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26698..27645
                     /gene="prmA"
                     /locus_tag="ACG8EF_000429"
     CDS             26698..27645
                     /gene="prmA"
                     /locus_tag="ACG8EF_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFS4945786.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27646..28410
                     /locus_tag="ACG8EF_000430"
     CDS             27646..28410
                     /locus_tag="ACG8EF_000430"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFS4945787.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28643..30856
                     /locus_tag="ACG8EF_000431"
     CDS             28643..30856
                     /locus_tag="ACG8EF_000431"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFS4945788.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30867..31313
                     /gene="dtd"
                     /locus_tag="ACG8EF_000432"
     CDS             30867..31313
                     /gene="dtd"
                     /locus_tag="ACG8EF_000432"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFS4945789.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31484..32743
                     /locus_tag="ACG8EF_000433"
     CDS             31484..32743
                     /locus_tag="ACG8EF_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFS4945790.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32832..33779)
                     /locus_tag="ACG8EF_000434"
     CDS             complement(32832..33779)
                     /locus_tag="ACG8EF_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4945791.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34173..35465
                     /gene="hisS"
                     /locus_tag="ACG8EF_000435"
     CDS             34173..35465
                     /gene="hisS"
                     /locus_tag="ACG8EF_000435"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFS4945792.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35480..37249
                     /gene="aspS"
                     /locus_tag="ACG8EF_000436"
     CDS             35480..37249
                     /gene="aspS"
                     /locus_tag="ACG8EF_000436"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFS4945793.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37358..38233
                     /locus_tag="ACG8EF_000437"
     CDS             37358..38233
                     /locus_tag="ACG8EF_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4945794.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38499..40382
                     /locus_tag="ACG8EF_000438"
     CDS             38499..40382
                     /locus_tag="ACG8EF_000438"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS4945795.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40457..40741)
                     /locus_tag="ACG8EF_000439"
     CDS             complement(40457..40741)
                     /locus_tag="ACG8EF_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS4945796.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40731..41465)
                     /locus_tag="ACG8EF_000440"
     CDS             complement(40731..41465)
                     /locus_tag="ACG8EF_000440"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFS4945797.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41594..42217
                     /locus_tag="ACG8EF_000441"
     CDS             41594..42217
                     /locus_tag="ACG8EF_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFS4945798.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42409..43530
                     /locus_tag="ACG8EF_000442"
     CDS             42409..43530
                     /locus_tag="ACG8EF_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS4945799.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43537..46665
                     /locus_tag="ACG8EF_000443"
     CDS             43537..46665
                     /locus_tag="ACG8EF_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HFS4945800.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46684..49878
                     /locus_tag="ACG8EF_000444"
     CDS             46684..49878
                     /locus_tag="ACG8EF_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFS4945801.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50024..50731)
                     /locus_tag="ACG8EF_000445"
     CDS             complement(50024..50731)
                     /locus_tag="ACG8EF_000445"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4945802.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16685 a         8919 c        10609 g        14786 t
ORIGIN      
        1 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
       61 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      121 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      181 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      241 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      301 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      361 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      421 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      481 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      541 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      601 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      661 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      721 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      781 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
      841 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
      901 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
      961 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1021 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1081 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1141 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1201 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1261 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1321 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1381 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1441 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1501 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1561 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1621 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1681 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1741 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1801 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     1861 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     1921 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     1981 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2041 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2101 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2161 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2221 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2281 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2341 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2401 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2461 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2521 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2581 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2641 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2701 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2761 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     2821 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     2881 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     2941 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3001 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3061 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3121 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3181 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3241 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3301 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3361 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3421 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3481 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3541 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3601 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3661 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3721 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3781 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     3841 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     3901 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     3961 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4021 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4081 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4141 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4201 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4261 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4321 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4381 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4441 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4501 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4561 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4621 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4681 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4741 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4801 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     4861 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     4921 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     4981 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5041 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5101 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5161 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5221 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5281 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5341 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5401 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5461 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5521 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5581 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5641 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5701 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5761 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     5821 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     5881 ttgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     5941 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6001 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6061 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6121 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6181 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6241 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6301 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6361 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6421 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6481 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6541 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6601 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6661 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6721 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6781 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     6841 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     6901 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     6961 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7021 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7081 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7141 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7201 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7261 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7321 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7381 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7441 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7501 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7561 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7621 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7681 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7741 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7801 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     7861 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     7921 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     7981 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8041 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8101 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8161 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8221 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8281 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8341 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8401 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8461 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8521 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8581 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8641 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8701 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8761 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     8821 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     8881 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     8941 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9001 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9061 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9121 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9181 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9241 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9301 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9361 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9421 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9481 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9541 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9601 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9661 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9721 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9781 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
     9841 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
     9901 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
     9961 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10021 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10081 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10141 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10201 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10261 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10321 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10381 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10441 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10501 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10561 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10621 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10681 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10741 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10801 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    10861 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    10921 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    10981 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11041 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11101 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11161 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11221 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11281 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11341 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11401 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11461 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11521 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11581 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11641 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11701 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11761 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    11821 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    11881 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    11941 gctttcataa aaacattaat atccataaat aaatctcaga aaatgaacat tacagatgta
    12001 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12061 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12121 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12181 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12241 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12301 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12361 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12421 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12481 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12541 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12601 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12661 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12721 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12781 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    12841 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    12901 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    12961 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13021 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13081 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13141 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13201 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13261 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13321 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13381 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13441 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13501 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13561 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13621 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13681 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13741 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13801 atttttataa gtataacata aattcggctt ttgccaatat ttttttgtaa attcatttta
    13861 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    13921 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    13981 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14041 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14101 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14161 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14221 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14281 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cgggacagtc
    14341 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14401 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14461 aaaatagcac agacttatga tacacttttg aaactcgcat tacggcatcg tccagacttg
    14521 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14581 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14641 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14701 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14761 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    14821 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    14881 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    14941 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15001 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15061 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15121 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15181 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15241 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15301 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15361 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15421 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15481 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15541 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15601 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15661 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15721 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15781 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    15841 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    15901 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    15961 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16021 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16081 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16141 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16201 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16261 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16321 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16381 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16441 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16501 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16561 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16621 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16681 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16741 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16801 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    16861 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    16921 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    16981 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17041 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17101 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17161 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17221 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17281 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17341 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17401 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17461 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17521 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17581 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17641 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17701 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17761 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    17821 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    17881 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    17941 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18001 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18061 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18121 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18181 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18241 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18301 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18361 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18421 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18481 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18541 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18601 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18661 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18721 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18781 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    18841 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    18901 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    18961 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19021 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19081 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19141 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19201 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19261 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19321 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19381 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19441 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19501 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19561 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19621 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19681 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19741 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19801 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    19861 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    19921 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    19981 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20041 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20101 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20161 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20221 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20281 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20341 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20401 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20461 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20521 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20581 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20641 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20701 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20761 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    20821 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    20881 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    20941 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21001 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21061 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21121 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21181 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21241 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21301 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21361 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21421 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21481 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21541 tctgttaacc gtgcttcctg acagtgtgga atccctaagc cttgcaagat cgctgcaatc
    21601 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21661 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21721 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21781 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    21841 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    21901 ccgttgatat cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    21961 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22021 ctccttgaga ccactcaaaa aggccatggt tatccctggc ctgtgttatt taaactcaga
    22081 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22141 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22201 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22261 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22321 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22381 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22441 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22501 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22561 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22621 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22681 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22741 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22801 ctggtgttca ctttttttat tttacttgaa ctcattgact gttttccctt tattttaaac
    22861 attataaaga ccgatgagtg cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa
    22921 aaaaacctgc cgatagttgg caggtcgtct acgtgttaag ctttggtgtg gtctttagct
    22981 ggcaattggt tttctaaatc agttcggaca acaagcacat cgcaagctgc gtttctaata
    23041 acatattcag aaacagaacc aataaataaa cgttctacag cattcaatcc tgtggctcct
    23101 aacataatca aatctacttg attatcttct gggatttctc tggcaataat ttgttttggt
    23161 gagccatatt ctacgacaga ggttacatta ttcaagccag ctttcttcgc attgctttca
    23221 taatctgcca atgtttgctt tgccatttca gtagcttgtt ctgcaagcat tccatcaaag
    23281 gatgaaacgg tttggaaaga acgtgtgtca atgacatgtg ctaatagaag ttctccgtta
    23341 tttcggatag ctacattgac cgccttttta aaagctaatt cagcttcatc agaaccatct
    23401 acggcaacca tgattttttt gtattgttgt aacataagca ttcactcctc aagtcaatcg
    23461 tattgtactc ttttattgta gaacacttta gggaaaaaga gaattattta ctgctttggt
    23521 tcagcaattt tttttgcaaa tcggatactg aaggtggcag aaacaataat tacgatcagt
    23581 aaaaagacca aggcaataaa gcgttgattg aaaaaagctg ttaaaatacc cattacgcct
    23641 aaaacagcat atgcagcacc gtaaaattgt aggaatcctt gatttttatc atttttgttg
    23701 atcaatacaa aaaagatatc ctgtttatta aataaataat agctaacgaa taataatact
    23761 aaagtggcga taatcattaa aatcctaatc atgaacaaat cctccaaaat aagcaacaaa
    23821 tagaaaaagc aatttgccaa gagcaaattg ctttattctt taaaagatgg taatctccgt
    23881 taatgccgtt gtttgtcctg tagtattgat cccgcgaatt caagcaggtg ggtcccatgg
    23941 tcgtagcttc gaactaccaa atgataaggc atgttaccag cagtgacaca agacgggtcc
    24001 caagatatca ataaaaatgt tcggtcaaca cacaaagagg acaattcgaa catcacagat
    24061 ttcccacctc tatagtagca tatgaattta aaaagagcaa tgagagagct cgcagaaaca
    24121 gaaccgtttt cttgtctagt tttttggagg atgctttttc cactctttta tacggtaata
    24181 ctgttgaccc agagcctgtt catatttccc tgtatctttt ggctgataat actgagcatt
    24241 cttcagttta tctggtaaat attgctgatt gacccaagcc tgatcaaaat gatgaggata
    24301 ctgataaccc acgccacggt tgagtgattt cgcacctttg taatggctat ctctcaaatg
    24361 atcaggaaca tctccagctt tgccctctcg aatatcagca agtgctgcat ctaatgccat
    24421 gtaggcagaa ttggattttg gcgacaagca aagatcgacg acgacgtctg ctagtgggat
    24481 acgtgcctca ggcaatccca atttttcagc agctaataca gcgttcactg tacgagccgc
    24541 agcagcagga ttacctaaac ctatatcttc ataaccgata accatcagac gccggcaaat
    24601 acttgccaga tcacctgcct caactaaacg ggcgagataa tgaagtgcag catccacatc
    24661 acttccacga atcgattttt gaaaagccga aataacgtca taatgagcat ctccattttt
    24721 gtcatgggta agagcttttc tttggacaca ttcttcaatg atcgacagtg taagatgaat
    24781 ccttccttct ttatctgaag gagtcgatag tgtagccagt tccagcccat tcaatgcact
    24841 ccgcaaatcg ccgttcgttg cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat
    24901 tgcttgttgt ccaaggccgc gttctttatc ccttaaagca tgttcgactg ccaactggat
    24961 gtcttgttct gttaatggtt tgacttcaaa gatctgtgta cggctgcgga tcgctggatt
    25021 gatggtaata taaggattct cagtagtcgc tccgatcaag atgattcggc cactttctaa
    25081 atgaggaagc agaaaatcct gttttgtttt atctaatcgg tgtacttcat ccaataagag
    25141 gatcactgta ccactcattt ttgcttcttc cgctacaact tgtagatcct tttttgaatc
    25201 tgtagcggcg tttagcatgc gaaatgcata attagtagag ccggcgatcg cacttgcgat
    25261 actcgttttt ccagtaccag gaggtccata tagaatcatg gaagagagca tgcgagcatc
    25321 gaccatgcgg cgtatgattt ttccttcccc gactagatgc tgctgaccaa cgacttcatc
    25381 gatcgttcga ggtctcatgc gataagccaa tggctgttgc ataggatttt cctccttctt
    25441 gtttttacgt cccttaatta tatcatattt agcaagatgc ctagaaagaa cagccttctg
    25501 ttgttttgtt ttcatagctt atccaactaa tattgtgtat gataaagtag gtgagaaaaa
    25561 tgacggataa acaaaagata gatgaatttt tttggaataa aacgacgcca gaagtggcac
    25621 gggatttatt ggggatgtat ttggagcatg acacaccaga aggaagatta gctggatata
    25681 ttgtagacgc ggaagcatac ctaggtccag aagacgaggc cgcccatagc tatggtttaa
    25741 gaagaacacc gcgtgtgcgg gcaatgtatg aaaaaccagg tacgatctac ctttacacga
    25801 tgcatactca tcggattttg aacatcatta ctcagccaga agggattcct caaggagtaa
    25861 tgatacgtgc aatcgaaccc gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa
    25921 aaactggtcc agatatcagc aacggtccgg gaaaattagt ggaagcatta gcaattcctc
    25981 aagaacttta tggtcagtct atagcagata gttcgttacg cttagttttt gagaaaaaaa
    26041 agacaccaaa aaagatcaat gctttgccta gaatcggtat cccaaacaaa ggtgtatgga
    26101 cagaaaagcc gttgcgtttt gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa
    26161 accaaatcga gaaagattgg ggttggagga aagaaaatga aaaagaagga tctattaaca
    26221 tatttagatg aaataattga aaaacaagtg acagactatg atgtagcttt agattgggat
    26281 acaaaaaatc atacgatcga gatcgttgtt cgcttgtttg cagaaaataa agcacaattt
    26341 gagattgatg atgcagaagg gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc
    26401 gtacttcttt ttaatcctca gaaatctgtt tttgatgaac gcaattatct agcagtaatc
    26461 ccttatgaag gaaaaaaagg gttggcaaaa tcagtagctg attctttagt ggaatatcta
    26521 aatgaagtat tagctcaagg gcaaagcgat ttgcttgatt ttttggatga agacgatgac
    26581 gaagctgtat tcgagcttca ctgggataat caacaactag cagaattagt agaagtaaaa
    26641 cagcaaaatg aagcagatac ttatttaccg tacccaagtt attaaggagt gacagatatg
    26701 aaatggaatg aagtaaaagt agaaactgaa agtgaagcag tcgaagcagt tgctaatatt
    26761 ttgatggagg caggggcgag tggtgtcgcg attgaagatt ctttagatgt tgaacatttt
    26821 cagtcggatc aatatggtga gattttagat aaaaatgatt ttacatcgct gaaagaaggc
    26881 gcactagtca tggcttattt tccggaaacg actttcttac cggaaatctt gccttttatc
    26941 aaagatcgta tcacacaatt acctgagttt ggtctagcta ttggaaaaaa taaagtaaca
    27001 gttagtgaag tagaggaaag caactgggca acagcctgga agaaatacta tcatccggtg
    27061 cgtattacgc gaatgctgac ggttgcgcca agctgggaaa actaccagac agaagatcct
    27121 ctagaaaaaa ttatcacact agatcctggt atggcatttg gcacaggcac tcatcccacg
    27181 acttccttga cattgacagc actagaaaca gtcttgcgtg gtggtgaaac attgctagat
    27241 gtgggaactg ggtcgggcgt gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc
    27301 tatgcctttg atttagacga agtagccgtt cgttcggcga aagaaaatat ggatatgaat
    27361 gaaatagcca aagatgtcca tgtatcagct aacgatcttt tgacaggaat cgagattgaa
    27421 agtgatgtaa tcgttgcgaa cattttagca gatatcatct tattgatgat cccagatgct
    27481 tggcgtttac tgaagcaaac aggcacgctg atcgtttcag gaatcattga agagaagaag
    27541 caaatggttc tggatgccat gttagaacaa gggtttatcg tggatcaaat tttccagcaa
    27601 aaagactggt atgcaattat tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt
    27661 ttaaaagaat catatgaatc aaaagactat tttgttttag aaggggaaaa ctatcaccac
    27721 attgtacggg ttatgaggat ggaacccgag gatgaagttt ttttggcatt caatgatcaa
    27781 atagccattg ttgcagaaat cacggagatt actgaagagg ctgtttatct gaaagaaatt
    27841 aaaaaagaag aaaatcaaaa ggaactgcca agttcgatta ccatagcgag tggctatcct
    27901 aaaggggaaa agctggattg gatcgttcag aaatccactg aattaggagc gcaagctttt
    27961 cttggtttcc cggcatcagc ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga
    28021 caacaacggc ttgaaaagat tgctcaagag gcagcggaac aatcccatcg gcagatcgtg
    28081 ccatcagtag agttattgga aacgaaacaa gatttatttg agcggctaga tgattatgac
    28141 gtggtcttgg ttgcttatga ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa
    28201 attcttagtg aaacaaaacc agatagtcga atcttagctg tctttggacc agaaggtgga
    28261 ttatcccgcg atgaagtcga ttattttgta gaacacaaag ggatactttg tggattagga
    28321 ccaagaatcc ttcgtacaga aacagcacct ctttatttat taagtgctgc tagctatcaa
    28381 tgggaattaa tgaatccttc tttcagctaa cgttcttcat taataaaaga gtatcgacag
    28441 aaagaattga aaaaatagtc aacaatttgt ataatagaaa caattattta tacaatggat
    28501 acgaaaaatc gtttgaaaaa gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac
    28561 tgcacggatt ttgggacaat tatcaaacag atatttgtcc cgattcccgt tttaccgaaa
    28621 aaagaaaggg ggatcgattc ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat
    28681 aaagctcgtt agtcaataca tgggtcccga acatgtagca tttgttgaga aagcatgcga
    28741 atacgcgact gctgcacatg acggacagtt tagaaaatca ggcgaaccct acattattca
    28801 tcctatccaa gtcgcaggta tattagcaga tttaaaaatg gatccccata cagtggctac
    28861 aggcttctta catgatgttg ttgaagatac agaaatcact ttagaagatc tgagagaaga
    28921 atttggcgat gacgttgcta tgttagtgga cggcgtaacc aaattaggga aaataaaata
    28981 taaatcccac gaagagcagc tagcagaaaa ccaccgaaag atgctgctag caatggctca
    29041 agatttacga gttatcatgg tcaaattagc tgatagacta cataacatgc gtacgttgaa
    29101 gcacctgcga gaagataagc aaagaaggat cgctcaggaa actttagaaa tctatgcgcc
    29161 tcttgcacat cgtctaggga tcagccggat caaatgggaa ttagaagata cagcacttcg
    29221 ttatctaaat ccaaaacagt attaccgtat cgtccacttg atgcagacga agagagaaga
    29281 acgtgaaaaa tacgtaagtg gtactgttga agatattcga atagcgacag aagagctggg
    29341 gatttttgca gaaatctatg gacggccaaa acacatttat tcaatctatc gtaaaatgaa
    29401 agatcagaaa aagcagttca acgaaattta tgacttgcta gctattcgag tgatcgtaga
    29461 ttcaatcaag gattgttatg ctgtattagg agcaatccac acaaaatgga aaccaatgcc
    29521 cggcagattt aaggattata ttgccatgcc aaaagctaat atgtaccaat ctttgcatac
    29581 taccgtgatc ggtccggcag gaaatccggt agaaattcaa atccgaacac aggaaatgca
    29641 tgaaatcgct gaattcgggg ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa
    29701 agtagaacca gatggtatga cgaaacaatt aagctggttc catgagatac tcgaacttca
    29761 agacgaaagc tatgatgctt ctgaatttat ggaaggcgta aaaggagata tctttagtga
    29821 taaagtctac gtcttcacac caaaaggaga cgttactgag ttaccaaaag gatccggacc
    29881 attggacttt gcatacagta tccatacaga tatcggtaac aaaaccactg gtgcaaaagt
    29941 aaatggcaaa atggtgcagc ttgattacaa attgaaaaac ggagatatca ttgagatcat
    30001 gacttctcca aattcatttg gcccaagtcg cgactggttg aaattagttg ctactagcaa
    30061 agcaagaaat aagatcaaac gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa
    30121 aggccacgaa tccgtggtca aatgtattac agatctagga tttacgccta aagatatttt
    30181 gacgaagaac aaactgcaag aagcactcga tcgttttaat tatcaaacag aagatgatct
    30241 ctatgcagct gtagggtatg gagaagttag ccccttgacg atggccaatc gtctgactga
    30301 aaaagaacgt aaagaacaaa aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa
    30361 tcagccgaaa aaagaacctg acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca
    30421 aggggtagaa aacttactaa ttcgtatcag tcgctgctgt aatccgattc ctggtgatga
    30481 tatcgttggt tatatcacta aaggcagagg gatatccatt catcgtcgag attgtccgaa
    30541 tgttcagcct gacaaaccaa atgtagcaga acgtttgatt gaagtcgaat gggaagatac
    30601 atcgaataca cgaaaagagt atgatgcaga tttggaaatt tacggctata atcgttcagg
    30661 cttattgaat gatgtacttc aaacagtcaa tgcgctaacg aaaaatctca acagcgttga
    30721 agcacggacg aataaagata aaatggcgac gatccatttg acggttggta tccagaattt
    30781 atcccaccta aagagtatcg tggataaaat caaagcagta cctgatgtct acagtgtacg
    30841 ccggacgaat ggataggagg acagatatga gagcagttgt tcaacgggta agtaaagctg
    30901 ctgtgtcagt cgatcagcaa gaaattggaa aaattggcta cggcctactt gttttattag
    30961 gcattcatga aaccgatacg caaaaagatg tggactatct ggtaaaaaaa attgcacaaa
    31021 tgcgtatttt tgaagatgaa caaggaaaaa tgaatattag tgtagaagat gtgggaggac
    31081 aaattttaag tatctctcaa ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa
    31141 gctttgtgga agccgcacga ccagaaacag ctattccttt atacgaatca ttcaatgaag
    31201 gaatcagaag tcgcggcatc acagtcgcta ccggacaatt cggagcagat atgtcggttt
    31261 ctctcgtcaa tgatggacct gtaacgatca ttattgattc taaaaacaaa taaagtaatc
    31321 tggttttcac tagtaaaaac tatgaaacac gtcgcttaag ttagactgat aaactctaac
    31381 ttaagtggct tttttgtctg caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta
    31441 agcttaaatt atacaagtta ttttttatag gaggttgctg aagatgaaag aagtaccagt
    31501 agtagatatc aaaatcacag atttaaaaaa attgtatcaa aaaggataca acatgttgga
    31561 ggagttacgc catgaagcgg acgctcccgt cgtcaaagca aagattttca ataaagaagc
    31621 tatcacgatc tacggatcat cagcagcaaa agtattttat gatccaagga atttcaaacg
    31681 aaaaggtgct atgccaaaat tagtattgaa gacacttttt ggtcaaggtg gagtacagac
    31741 tttggatgga gctgctcatc accatcggaa aaatattttt atggatctaa tgacaccgga
    31801 aagaatggaa gattatcatc gtatcttaga taagaatctg acacaagcac ttgaagcaca
    31861 gcatggtcaa tttgaactat ttgatttaag taagatggtt ttctttacat ctatttgcga
    31921 gtgggcaggt atcaatttat cagcgatttc taaagatgag gtcgaaaaat tagcagaata
    31981 tcaaatctct atgatcagcg gaacgtttac ttctccgatt gatcacataa aaggtgtcga
    32041 gaatcgcaaa aaatcagaaa aatgggcgca aggcttgatt gaagaagctc gacaaaatcc
    32101 tgtggctgga aaagaaaatg tggcattata tgcttttgcc aatgccacag atttagatgg
    32161 tcagctttta cctctggaag tggcagctgt tgaattattg aacatcatcc gtccaacggt
    32221 tgctctaact gtgtgggcgg cattgatggg acatgcactt ttcagtagac cagatttgta
    32281 tcagcaatta aaaaatgact tttcgacgtt acaagatccg tttattcaag aaatgagacg
    32341 ctattatcct ttcttcccaa tgctacctgc gatttcgctg aaagaagtag aagtagatgg
    32401 ttatcgtata ccagaaggca gttgggttat tttagattta tatggaacag accatgatga
    32461 acgaacagtt gaagcgccag attcctttat gatcaaacgg tatgtaggga aagcaaaaga
    32521 tatttcctat aaagaagaat atgagatgat tgcacaaggc gggggaaatt ttcgacaaat
    32581 gcatcgctgt gcaggagaat ggatcactct tcatagtcta cgtgtgtttt ccgatcagct
    32641 agtaaataag tttgagttct ctgtacctga acaagattgg acgattccat tcaatcagtt
    32701 tccgacttat ccgaatagcc gagcgctact ttacaagaat tagattagca taaacttatc
    32761 gaaaaaaagg aagatcaaac agtgctgatc ttcctttttt catattacta taaattgata
    32821 aggtcaattg atcaaaatcc acgaaatgct tcatcaatgg tttgataatc ttcattggat
    32881 aaatgcaaat ctaaagcttg gacattgtca gatacttgct ctggacgctt agctccaggt
    32941 ataactacag acactcgtgg attcttcatg taccaagcta aaacaagttg cgcaggagtg
    33001 gcttggtatc ttttagcaat ggggcgaagt acatcgacag cggttaaaat ttctctgaaa
    33061 cgttctcctt ggaaatctgg atttcttttt cgaggatctc cttcaccaaa ttgtttttcc
    33121 tcaccaagtt catattttcc tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa
    33181 ctgattttgt ttttttcaag ataagggaat aattcttttt cagcctgccg atggatcaaa
    33241 ctgtatttat cttctactac atcgacatat ccatctgcat tcgcttcttt caattgttct
    33301 aacgtgaagt ttgacacacc gactgctcgg atttttcctg cttcttttaa ttcgtgcaat
    33361 gtagcaactg attcattttt tggtgtgctt tcatccggaa agtggatata aaaaatgtcg
    33421 atgtaatcag tttgcagacg ttttagtgca tcttccaccg attgtttgag aaactcagga
    33481 gaattatcaa atgttcgccc tttatttggt acatgagcag cttttgtagc gatgacgaca
    33541 cgtgagcgat catattcttt ttccttcagt acctcgccaa tcagttcttc ggaacggccg
    33601 tttccgtaag cataagcggt atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact
    33661 acttgttttc ctgtctcatc ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt
    33721 gctcctaatc caagctgttc tgcgtagact tgtgaatgtc cgattcttat ttctgccata
    33781 gctgattcct cctttttgtc tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt
    33841 tttgtgaggg gaaatagtca gatatctctt gactttgaga ctggttttta gtaggttata
    33901 agtaatatcg aatgtcaatg aaagagcgag taagtttttg aaactttaca agagaggatc
    33961 atcgaggctg aaaatgatct aaagtttcat tagcctaaag acactctgga gatggacgag
    34021 tgaaaagccg tcccgcgtac gagcgttatt cgtaagagga aagagaaatc tttcaaaatt
    34081 aggtggtacc gtgtatgaat ttcattcacc cttgttcatg atgaacaagg gtgatttttt
    34141 tatttacata gctaagaaag ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa
    34201 atgatatttt gccaggcgaa tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat
    34261 tattcagaga ttatcagtat aatgaaatgc gtacaccgat ttttgaacac tatgaagtga
    34321 tatcccgaag tgtaggggat acaacggata tcgtttcaaa agagatgtat gatttttatg
    34381 acaaagggga acgccatgtg acgcttcgac cagaaggtac agcaccaatc gttcgttctt
    34441 ttgttgagca taaattgttc ggcccagagt atgcgaaacc gttcaaaact ttttatatgg
    34501 gaccaatgtt tcgctacgaa cgccctcaaa aaggacggtt acgtcaattc catcaaatcg
    34561 gagtagaagc atttggatca gaaaaccctg caactgatgt ggagagcatg gtgatggcac
    34621 ttgatttctt caaacagttg gggatcacgc aaatccggct agtcatcaat tcattaggtg
    34681 ataaagaaac gcgtcaagcc tatcgtcaag cattgatcga ctatctttta ccttttgaat
    34741 cagagctgag tgaagactct aaacgccgcc ttcatgaaaa tccattacgc gtattggaca
    34801 gtaaagacaa acgagatcaa aaatttgtgg cagaagctcc atctatctta gattatctaa
    34861 gcgagcctgc tcaaaaacat tttgataccg ttgtttccat gctggatgca ttggctgttc
    34921 cttatgaaat cgacagtaat atggttcgcg gactagatta ttacacacat acgatttttg
    34981 aaatcatgag tgacgcacca aaaatggggg cacaagcaac gatttgcgca ggcggacgtt
    35041 atgacaatct agtggaagaa ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg
    35101 gtatcgagcg attactcttg actatggagg cagaggaagt agttatccct gcatttaatg
    35161 agttggatgc ttacgtggtc gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg
    35221 tccaagcgat tcgaaacttt ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa
    35281 aagcgcaatt taagacagca gataaagcga atgccaaatt agtattggtg atcggtgaag
    35341 atgagcttgc tagtcaaacg attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt
    35401 ttccattaga agagatatat gaacattttg atgaagtcta tgatgaaatg actttattcg
    35461 agtaggagga aaaaaataaa tggcacaaag aacgatctat tgcggattag tttccgaaca
    35521 acaattagaa caaacagtta cattaaaagg atgggtacaa aaaagacgag accttggggg
    35581 agtaatcttt atcgacctac gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga
    35641 aatcaacaaa gaagcatggg aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt
    35701 gactggggaa gttagaagac gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga
    35761 attcgaagtg attgcaagtg aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca
    35821 aattgaagat gatcaagcaa tcagtgacga aatacgtttg aaatatcgtt atttggatct
    35881 gcgtcgtcca aaaatgacta aaaacatcaa attaagaaat gcgacaacaa aagcgattcg
    35941 tcattattta gatgataatg actttctcga tatcgaaaca ccttacctag ggaaatctac
    36001 accagaaggc gcaagagact acctagttcc ttcacgtgtc catgctggac atttctatgc
    36061 gttgccgcaa tctcctcaat tgtttaaaca attgctgatg aatgctggat tcgaccgtta
    36121 ttatcaaatc gttcgttgtt tccgcgatga agatttacgt ggagaccgtc agcctgaatt
    36181 tacgcaagta gatattgaaa caacgttctt aacagcagaa gaaatccaaa cttacacaga
    36241 gggattgatt gcgaaggtaa tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc
    36301 tcgtatgacc tatgatgaag caatggctcg ctacggcagc gacaaaccgg atacacgttt
    36361 tgatatggaa ttgatcgatc taagcgatac agtcaaagaa gtagaattca aagtcttcca
    36421 aatggctctt gaaaacggcg gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg
    36481 ctactcacga aaagacatgg atcagttagg tcaatatgta ggacaattcg gagctaaggg
    36541 gcttgcatgg ctgaaagtag aagaagacgg attgaaaggt ccaattgcta aattcatggg
    36601 agaagcaaca gaagcaatca ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt
    36661 tggcgcagat aaaagtgaaa tcgtcgctgc tgcattaggt gctatccgga cacgtttagg
    36721 aaaagaatta ggactaattg atgaatcgaa attcaatttc ttatgggtaa ctgattggcc
    36781 acaatttgaa ttttcagaag aagaaggtcg atatgtctct gctcaccatc cattcacgat
    36841 gccaaaagaa gaagatatcc cgttattggc tgaagaccct gcaaaagtct atgcagaagc
    36901 ttatgatatc gtcttgaacg gttatgaatt aggcggaggt tctatccgta ttcatactcg
    36961 tgagcttcaa gaaaaagtac tagaaactct aggctttaca aaagaacaaa tggaagaaca
    37021 atttggtttc ttgttaacag ctttagatta cggattccct ccacatggcg gtattgcatt
    37081 aggattggac cgttttgcaa tgttattagc cggagaagat aatattcgtg aagtcattgc
    37141 ctttcctaaa aacggaaaag cagcagatgt gatgagtgat gcaccaagta tggtttcgcc
    37201 tttacaattg ttcgaattga atattgatgt aacgaatata gaagaatagc cgataaaaag
    37261 gtaaaaagga tttgtcgaat acgcttcttt tgacaacctt gatttaattg aaaaacactt
    37321 tataatgaag ccataacata taaagcgggg cttttccatg aaaaaaacat tcgaaaattg
    37381 ggcttatcat gattacacga ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc
    37441 agtcgcattg aatttctttt atcagccagg acatatttat tcaagtggta ttactggtct
    37501 agctcagatt ttgacgactt tgtcaaaatc actgatagga ttcgaagtac cggtatcaac
    37561 aactttgtat ttactgaata tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa
    37621 gtttacaatt ttcactttta tcacagtcac tttgacctcc ctttgtatcc atattttacc
    37681 ggaaacagtc ctatctaccg atccaatcat ctgtgcgttg tttggggggg ctgtgatggg
    37741 actaggtatt gggctagcgt tgagaaacgg cctatcatcg ggcggtctag atatcgtgag
    37801 tatcacgatt cgtaaaaaaa caggaaagtc gattggttct atttcgatct acttcaatgc
    37861 attgatcgtt tttgttgccg gaattctatt tggctggcaa tatatgtttt acagtacgtt
    37921 gtctattttc gtcagtggaa aagtaactga tgtcgtttat actaaacaaa agaagatgca
    37981 ggtaatgatc gtaacgaaac atccagaaaa agtagtaaat gaaatccaaa actgtatgcg
    38041 tagagggatc acgatcatca atgaagcaga aggtgcttat aagcatgata agcagacagt
    38101 attgatgacg atcgttacac gatacgaact gcctctactt agagaagcaa tgaaagcttc
    38161 tgatccacaa gcttttgtaa gtattgcaga aaacgtccag atacttggtc gtttctatga
    38221 ggatggcatt tagatcgagt ttgacaaaaa ttcatgaaat actgctgtag acagacgttc
    38281 gtttgtctgc agtttttttc taccatagta gaatcagcaa agaaaaactg cttttttatt
    38341 tgaaattttc ttaaagttga aaaaaagcat gtttattttt ttaaatttat gggatgatag
    38401 aaaaaaatag tagagaaagg acacgattct acttgctatt ttcacttaat acccgtatat
    38461 tacgaatgaa aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa
    38521 aaaacagccg ctacatcact gggtttgatg ggctccgttc attagctgtg gtgggtgtta
    38581 tcttctatca ccttctgcca actagtatga aaggcggcta tttgggcgtt ccgatttttt
    38641 tcgtcgtatc tggttatttg atcacggatc tgctgcggca ggaatgggaa caaaatggaa
    38701 aaatcaatat ttggcaattt tatgtcagaa gaatgaagag actctatccg gcacttgttt
    38761 ttatgcttgt gacagcttcg gcttatatca ctttgtttca gcgagggctg ttaaacaatc
    38821 ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa atcaataacg
    38881 gtttatctta tttcgatcga tttgctaatg agtcaccgtt tacgcatatc tggtctttag
    38941 cagtagaagg tcaaaactat ttggtttggc cgatcttgtt tgtattgttg atggtatttg
    39001 ttcgtaaaag aaaatggatc ttttatactg tgttaggtac atccttcctc tccgccatct
    39061 tgatgatgat cttgtataca ccaggaggcg atccaacccg tgtttattac ggtaccgaca
    39121 ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt
    39181 taaagaaaaa catcccgaaa caagcgaaaa atattctgaa tatcgctgga ggaatctcgc
    39241 ttttggcact gattctttgc ttcttctttt tagatgatca ttacaatttc atctattacg
    39301 gaggaatgct cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg
    39361 gagcaagttt gaatcgatgg ctgacaaatc cagtatttac ttggattggc aaacgaagct
    39421 atggcattta tctgtatcag tttccagtta tgatttttta tgaagcaaag gtgactgata
    39481 ttgccgatca tgttttgttg catactttag tggaaatcac attgattcta atcatcagtg
    39541 aactttctta tcgatatgtg gagcgtccgc tagcacgttt tgattatggt cgtacattcc
    39601 aagtgatgaa aggatggttc agcaaaccga tcatcagtaa gagcaaacca tgggttgttc
    39661 caggcacatt acttgtagca attgcactcg ttggtttcgt tacggcaccg aaaaacgctg
    39721 taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag
    39781 aaaccagaaa caattcagaa aacaccacta gttcggttga ccaagcgatc cttgataagt
    39841 acgagttgtc caaggcacaa gggaataagg cacaaaaatt agaactaacg gcatttggtg
    39901 attcagttat gttggatgct gcaacggatc tacaagaact ttatccaaaa gtagtggtag
    39961 atggagatgt cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac
    40021 aagatttgtt gaaagacact gttctgattg gactaggaac gaatggctca ttctcggaag
    40081 ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc
    40141 gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa
    40201 aatacgacaa tatgacgctg attgattggt acagctacag taatgaccat agtgactggt
    40261 tttatgatga tcaagtccat ccaaatcctg aagggatgac acattacatt cacttagtca
    40321 gcgaagcctt gctaggagga agttcaagta cgaacagcag tgagacaaac gtgacagaat
    40381 gatagctaca aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt ttttatattt
    40441 accgtgtttt taacattcat atagattcag gaagcaaaac ggttgagtgt gtctgtaaat
    40501 aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc
    40561 ttcttgaaga aaatacttct gcatggatca tcttaacagg tcttcttttt tgaagacgag
    40621 tatatttagc accagtacca ttgttatgtt cctctaatcg tctagaaaga tcggttgtat
    40681 atccaccata aaatgtctga tctttacaga ataatacgta aaaataatgt tcatttgcca
    40741 tagagcatct cacgaatctc tgcagtgtat tctccatcca tattgtaagt gatcaatggt
    40801 ggagcgactt tgaatccgtc taatttgcct tctttgattc cttcaactaa caaaatattt
    40861 gcctcttttc caggttttgg gtaaacaaat tggatccttt taggtgcaat tcgatagtct
    40921 tccatgacct gaatgatttc taagaaccga tcaggacgat ggaccatagt aaagcgtcca
    40981 tttgttttta gtagcttact tgaaatatcg atcacttctt ctaacgtcgt atgaatctca
    41041 tgacgggcga ttgccaggtg tttattaggg ttcgttttat ttgttggcag ccctttgaaa
    41101 taaggtggat tacagacgac taagtcacaa gaattatgtc gaacgacaga taagctgtct
    41161 ttcagatcga tggtgtggac agtcatttgt tcatttaaat ggttaagcgc gatactacgt
    41221 cttgccatgt ctgctagacg aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt
    41281 ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat cttccctttt
    41341 ttaggtacac gggaaaagtg agcaagcagc acagcatcta aagaaaaaga aaacactttg
    41401 gaactttgga taatcttgat atcttctgcg tataattggt cgattcgttc gtcttcgtga
    41461 agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt attatactat ttttattaaa
    41521 aagaaccaag gtggcttgtc aaacctctat tttttatgca tactatgacc ataagaaggg
    41581 aaaggaagag actatgtttt atcgatttat gcgcggggta gttcgagtac tattagcctt
    41641 agttaatgga aatgcccgct atgagaataa agaagtttta ccgcaagatg agaattacat
    41701 actagttgct ccccatcgta cttggtggga cccattatat ctggcggtag ctgcagcacc
    41761 caaggaattc agctttatgg caaaagaaga actgttcaaa aatccagttc tgcgatttat
    41821 attaactcga tccaatgctt ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa
    41881 aacacctgta aagattttga aatcgacaaa tttgagtttg atcatgtttc ccagtgggac
    41941 aagacatgct acagaactta aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa
    42001 aatcgtgcca gctgtatatc aaggtccgtt gacacttggg gatctgttca agcgtcagag
    42061 agtgacagtg cgtttcggcg aaccaatcga cttatcagat atcaagaaaa cggataaaga
    42121 aggtatggcg gagatcgaac gacgaacaca agcagctttt gacaaattgg acaaagaagt
    42181 gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg
    42241 gtcttttgaa tctactgtcc tgttcagttc agactttttc aatgaaaacg agggagagac
    42301 gtcaagtctg ccctcttttt gcgttccaat atgtaaaata gaagacaggt gtcatgtaat
    42361 ggatcaaagt gttataataa ttagaaggac attttttgag gtgattgaat gcgtttttta
    42421 catacagccg attggcatat cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag
    42481 cgtcatgtac tatcttctat actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg
    42541 attgccggag acttatatga taggagtgtc ccagctgttg atgccgtaga attatacaat
    42601 gaattgatgg tcgattggaa tttaaaggaa aagatcccga tttttgctat atcaggaaat
    42661 cacgatagca gtacgagact ttcagcgggt acgccatggt tcagttattc gaactatttt
    42721 ttgcgaacac agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc
    42781 tttcttcctt actttgaacc aattgcagcg agattatatt ttgaagatga caatatccga
    42841 acgatcaagc aagcggtagc caaggtgata gataaaatgc aggagctatt tgattcagaa
    42901 aaaaaacaag tattgattag ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa
    42961 acgaaaatcg aagtaggcgg gttggattcg atccctggtg aactacttac accgtttgat
    43021 tatgtagcac taggacattt gcataatcaa gctgctttga gacaggataa tgctcgctat
    43081 agcggttccc cattgaaatt ctctttatct gagatcaacc aaaaaaaagg cgtatggatc
    43141 gttgacctga caacaggcct ggagttgaat tttagggaga tcgaacccct gcatgatatt
    43201 gaagaaataa cagcgagttt caaagaattg ctggatcctg ccttttatca gtccgtcaat
    43261 cgggagaatt acatctatat ctatctcact gatagaacga tcatcccgaa tatgatgaac
    43321 cagcttcggc aaatatatcc tcgtattttg gggattgaac gggcaaacgg tcgagcagaa
    43381 caaacaaagc gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt agcagaagca
    43441 ttttttagtg aaatgacgga agagcagcta actgcaaacc aagaaaaatg gctcggagaa
    43501 acgttgcaag aaatcatgga aatggaatga tcaagaatga aaccgaaaaa attacaactt
    43561 aaaaattttg gtccttttat caatgagacc gtagattttt cacgtttgac agaagcaccg
    43621 ctttttttga tcagcgggaa gacaggagct ggcaaaacga cgatttttga tgggatcacc
    43681 ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg gaaaagagat gcgttctttg
    43741 tttgctacac cagacgaaga aacaagcgtg actttttctt ttgaacatca gcagatgagc
    43801 tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa
    43861 aaacaggcag ccaaagtaac gttgaccatt tttgatggga aaggaaaaga aataaaacaa
    43921 atcacaaaac gtacagaagc agatcagttg atcagagaac tgatgaatct cgatgcgaaa
    43981 cagttttctc aaatcgtttt actaccccaa ggagaattcc gtaatttttt ggtttcatca
    44041 agcagtgata aagagactgt tttacgtcat ttattcggta ctcaattttt ccaacagttc
    44101 aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa aattggatca tttagagcaa
    44161 gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg
    44221 tttgaagccg ttttagccca atggacaaac catcaggcaa tactcgaaaa acaaatcacg
    44281 gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa agcaactaga aaattcctac
    44341 tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa
    44401 aaggaattag ctgctcaaaa agacgaaata gaggaaaaga aacgatggat tgggtattat
    44461 gaatttacgc aacgattagt cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa
    44521 atagaactgt cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac tacgcagaat
    44581 tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa
    44641 gctgctcaaa aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc cttacgtagt
    44701 aaaaaacaag acgcagaaca attaacaatt tcattggata aagaagaatt gaatgccgag
    44761 aagctgtatc aaaatctcgc tatctatcag gaaagaactc gaattcttca agaaaattta
    44821 ctggatcagg aaaaactgca agaagatcgt cttgcttttg gacagttaaa atatctagat
    44881 gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata
    44941 agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct attgaaagaa
    45001 cagacagaac aagcagaaca gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt
    45061 cagctttttt tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga acaccccata
    45121 caacaattaa aacacgaaca gccaaataag gaagatatct taaaagtaga acaacaactg
    45181 gcagaagctg agtctgctgt agaaagaact cagcaaatca ttgccaaaag tgaagcacat
    45241 gaaaataatc ttcaacaaca attatcagat ttagaacaaa aaatcatggc aacgaatata
    45301 caattggctg aacaagaaga acacttgcaa caggctttga aaaaggcatt tcctgagata
    45361 acagaacaaa aaacaaaaac aactcttgta gtaatcgaac aatctttaga tagacgaaaa
    45421 agcaagatgg ccaatgaaag aatagagctg gaagaaataa gacaacaagc agaaaagctc
    45481 gaacaacaat tagctgtcta caaacagcag cagacagaaa aaaataatca gctccagcaa
    45541 ctgcaaggag aaatcaaaag tttagaacaa caattgcaag atacggatag taactgttta
    45601 gaagaggaga tacgacgact tgaaaaacag ttagaagaag actcgaatca actagctgtt
    45661 gagcagaaag caggagtgca attgttgcaa gatcttcata cgcttcggac acagcttgat
    45721 cttcaacatg aacaaataga aaaaatagat gaaaaaatag aaaaggaaca agaaagaata
    45781 gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg aagaaatctg tgcatttgca
    45841 caacagcaag cacaatataa agaaaaccaa cgtacagtta cttcttatca tgaagagcaa
    45901 ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc agctaactga gacattccca
    45961 gatacagaag agatcagcca acagttagaa gaaataagac gtatgatcga aataaagcaa
    46021 gaaaagatat acggtttaca agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt
    46081 ctttcgattt atcacacaag ccagacgaaa ttagatgagc ttacgcaatt acagcagctt
    46141 tcgcaaacat tcaacggaga aaatcctaag aagactagtt tggaacggta tgtgcttcag
    46201 gtttatcttc aagaagtgtt acaagtagca aatgatcatc tccaacgttt aacgaaaaat
    46261 cgttatcaat ttgagctagc ggataccatt ggcagttatc gagggaaaac cggattagaa
    46321 atcaatatct atgatgatca ggcaggtatg tctcgaagtg cgcataccct gtctggcggg
    46381 gagagtttta ttgcagcact ttctttagca ttagctcttg ccgatgtgat ccagacacaa
    46441 gctggtggtg tagcaatcga tgcattgttt attgatgaag gatttgggtc attagatgaa
    46501 gaagctttgg aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc
    46561 ggcatcatta gtcatgtaag agaattgaaa gaacgagtat tacaacaaat aagaattgat
    46621 acagaaggta gcggacaaag caaaatacgc tatcaacttg gataaaaagg gagattggta
    46681 actatgaaat ggagtattcc agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa
    46741 ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg
    46801 ggcagtgaat tgtatatggt tgatttggat ggtacagcaa aagagataga ttactgccaa
    46861 tgtccctatt gggaggaaca taaatattgt aagcatacag ttgctgtgga actttattta
    46921 cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc caatgctaga accggtaaaa
    46981 gcaacaagtg agggagaaat cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat
    47041 cctcaagcgg tgcaaccttt gattattgaa taccaagtag aaacgattga gactaatcag
    47101 tatcacccag agctttctat tttggcactt tacttgcgta tcggttcatt ggcatcaccg
    47161 aaaaaaacat atatcgtaaa aaacatctat gattttttac aagcttacac agaaaaagaa
    47221 acttacactg ttaataaaca gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa
    47281 gatcaaaaaa tactcacgca tcttgcagga agtgctcaga cgcatcaatt attaggtacg
    47341 aacggcttac aagtcaaagg aaaactagat aaaagatatc tgcttctccc agtagaacaa
    47401 gcccgcttcc tgttagagca gatgaatcag actacacgtt tgcatctgca gataaatgaa
    47461 caaaacgatc aaggaataca attttctgat caaaacaaac ctttatcatt caaagtggaa
    47521 aaatttggag aagattattt gctgcaagca ttgaatgatt ttgatttttt ctttatgcat
    47581 tatcagtggg gagttatcca agggacattt tattctctga cgaaatatca acaaacggtt
    47641 tatcaaactt ggaaacaatt gattcgccga ttggagaaac cagaagtagt gttcaagaaa
    47701 aaagaattac cgtctttgtt taaagagatt atccctctgc tgtcagagat cggcgacgta
    47761 gctattgccc ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt tattttcttt
    47821 ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc
    47881 tattcgactg acccgaagca tgaggtctcg acagaacagg cttttcaggt gttacgtgac
    47941 acagtagaag agaaacgagt acttgatcta ttcagaatgt atcgctacca gaaaaatgaa
    48001 actggttatg aacgtccatt gccaagcggg gaagaactat atgccttttt ccgcacagaa
    48061 ctatctgtct tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg agagttgtat
    48121 ttagatgcgc ataaacatca accgaaaatc gaagtaaccg agaccggttc atggctcgat
    48181 attcgattcg atgtgacagg gatagaggag caagaaatcg atcatgtcct gcaaagcttg
    48241 ctgagaaatg atgcttttta tacattagaa aatggtcagg ttctttcttt tgattcagaa
    48301 gaatttcaac aaacaagcca agtgttgcaa caattacggg aatcgatacg tacagaagaa
    48361 ggaacgatcc atgtacctaa aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc
    48421 aatgctactt ttagtgaatc gttccaaacg atggtacaag atctcattca tccagaaaga
    48481 taccaggcac agcttcccaa aggattgaat gcaacgatga gagattatca aaagcaaggt
    48541 tttcgatggt tgaagatgtt aggtcattat cagtttggcg gtattttagc cgatgaaatg
    48601 ggacttggga aaacactaca gacaattgcc tttttacttt cggaaaaaga agaacggaaa
    48661 agcttctctg ctttgatcgt agcgcctgcg agcttaattt acaactggca ggcagaggtg
    48721 agaaaattcg ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa
    48781 gaattactgg ctaaagacac agatatccga gtgacttctt atgccagctt aagacaggat
    48841 ctagctaatt atcagtcgca aaaaattgat tatttgattc ttgatgaggc acaaatggtc
    48901 aaaaatagca gtacgaaaac tgcacaagca ttgagagaat tagctgtacc tcagcggttt
    48961 gcactgagtg ggacaccaat cgagaataat ttagaagaat tgtggtcatt gtttgcaacg
    49021 attatgccag gatttttccc gactaaaaca aaattcaggg aacttgccgc agaagaaatt
    49081 gcgcaaatga ttcgaccgtt tattttacgg cgagataaac aaactgtgtt gaaagacttg
    49141 ccggaaaaaa cagagatgaa tctatattct gcacttacag aagaacaaaa aactgtttat
    49201 ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga agcattcaag
    49261 aaaaaccgga tcgggatact tgctggtctg acaagactgc gacaaatttg ctgcgatcct
    49321 cgtctgttta ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta
    49381 ctggtagctg ccaaagagaa caaacgaaga gttttgctgt tttctcaatt tacagggatg
    49441 ctgactatct tgcaagaaga gttagccgag ctagggatct cgacatttta tttaagaggc
    49501 agtacgaaac cacaagatcg gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat
    49561 gtttttctga tttccttaaa ggctggggga actggattga atctcacggg agcagatacg
    49621 gtcatcttgt atgacctttg gtggaatcct gccattgaag aacaagctgc tggacgtgca
    49681 catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga aggaacgatt
    49741 gaggaacgca tggattcact ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa
    49801 ggtaatgaag aacagttgac gaaactgact gaagaagaca tccgcatgat tttaagtgta
    49861 ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt aatgggtaag aaagcctttc
    49921 taaagaagcg attgtttatt tgatttaaaa aatagcaagg aggctgggat tccattttat
    49981 cccagcttcc ttgctatcat ttttttattt ctatttttaa aggttataca aagattgtct
    50041 tcattgtctc taacagctga tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt
    50101 caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta
    50161 gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc atatgaaatt
    50221 ctttctcagc cagctgaaaa atctcttttt ctggtttttg gaaacctgtt gcttgagaaa
    50281 tgagcatatt gcgggaagga atccaattgt tcaattgaag ttgcttcagt ttttttgtct
    50341 gatggtcagt cggcccattt gtgataattc ctaatgggac attcttttcc ttcaagaaat
    50401 cgagggtttt tttgacctcc tcatgcaagc agatattatc aagttcttcc tcgtaaattt
    50461 tttgaaataa gaggccatct tcttccgtaa tatgagggta gtctaaatct tttagtgatt
    50521 ggctgatccg atgtgctcgc atatattcta gtgtccaatc tcctgccatt acttttggga
    50581 agttttcatc actatgatgg cggaaccgga tatatagtgg atgcatatct gcatctgaaa
    50641 caagcggaac gactcttttg accgcgtttc gaaatggctg ctgctgatca tacatcgtat
    50701 catcaacatc aaacacaact gatttaatca aaattttaca cgcctttcgg ttttcattga
    50761 ttttttcaca aatccttgac tattgtaaca gaaaaaattg tctaaaaaag ctgataaata
    50821 aaggtttttc ttactatatt tctatttcct tgacagaata aggtttttat atttcagttt
    50881 ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa tcattatggt
    50941 agaagtcgat tgatttattt taatgcaatc tctgtggaat tttcgaaaga tttattttt
//
LOCUS       DAZUKZ010000008        50511 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000008 DAZUKZ010000000
VERSION     DAZUKZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.008"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(230..1957)
                     /locus_tag="ACG8EF_000446"
     CDS             complement(230..1957)
                     /locus_tag="ACG8EF_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFS4945803.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1950..3143)
                     /locus_tag="ACG8EF_000447"
     CDS             complement(1950..3143)
                     /locus_tag="ACG8EF_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4945804.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3330..3974)
                     /locus_tag="ACG8EF_000448"
     CDS             complement(3330..3974)
                     /locus_tag="ACG8EF_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4945805.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4068..4202)
                     /locus_tag="ACG8EF_000449"
     CDS             complement(4068..4202)
                     /locus_tag="ACG8EF_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFS4945806.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4204..6336)
                     /gene="feoB"
                     /locus_tag="ACG8EF_000450"
     CDS             complement(4204..6336)
                     /gene="feoB"
                     /locus_tag="ACG8EF_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFS4945807.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6333..6806)
                     /locus_tag="ACG8EF_000451"
     CDS             complement(6333..6806)
                     /locus_tag="ACG8EF_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFS4945808.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7207..7431
                     /gene="nrdH"
                     /locus_tag="ACG8EF_000452"
     CDS             7207..7431
                     /gene="nrdH"
                     /locus_tag="ACG8EF_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFS4945809.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7590..9749
                     /gene="nrdE"
                     /locus_tag="ACG8EF_000453"
     CDS             7590..9749
                     /gene="nrdE"
                     /locus_tag="ACG8EF_000453"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS4945810.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9799..10764
                     /gene="nrdF"
                     /locus_tag="ACG8EF_000454"
     CDS             9799..10764
                     /gene="nrdF"
                     /locus_tag="ACG8EF_000454"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS4945811.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10853..11992)
                     /locus_tag="ACG8EF_000455"
     CDS             complement(10853..11992)
                     /locus_tag="ACG8EF_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4945812.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12301..13014)
                     /locus_tag="ACG8EF_000456"
     CDS             complement(12301..13014)
                     /locus_tag="ACG8EF_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFS4945813.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13268..13969)
                     /gene="nagB"
                     /locus_tag="ACG8EF_000457"
     CDS             complement(13268..13969)
                     /gene="nagB"
                     /locus_tag="ACG8EF_000457"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFS4945814.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14203..15735
                     /locus_tag="ACG8EF_000458"
     CDS             14203..15735
                     /locus_tag="ACG8EF_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4945815.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15926..17128)
                     /locus_tag="ACG8EF_000459"
     CDS             complement(15926..17128)
                     /locus_tag="ACG8EF_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4945816.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17210..17899)
                     /locus_tag="ACG8EF_000460"
     CDS             complement(17210..17899)
                     /locus_tag="ACG8EF_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4945817.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18005..18373)
                     /gene="rplL"
                     /locus_tag="ACG8EF_000461"
     CDS             complement(18005..18373)
                     /gene="rplL"
                     /locus_tag="ACG8EF_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFS4945818.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18426..18929)
                     /gene="rplJ"
                     /locus_tag="ACG8EF_000462"
     CDS             complement(18426..18929)
                     /gene="rplJ"
                     /locus_tag="ACG8EF_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFS4945819.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19133..19260)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19339..20028)
                     /gene="rplA"
                     /locus_tag="ACG8EF_000463"
     CDS             complement(19339..20028)
                     /gene="rplA"
                     /locus_tag="ACG8EF_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFS4945820.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20132..20554)
                     /gene="rplK"
                     /locus_tag="ACG8EF_000464"
     CDS             complement(20132..20554)
                     /gene="rplK"
                     /locus_tag="ACG8EF_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFS4945821.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            20897..21565
                     /gene="sdaAB"
                     /locus_tag="ACG8EF_000465"
     CDS             20897..21565
                     /gene="sdaAB"
                     /locus_tag="ACG8EF_000465"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFS4945822.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21578..22450
                     /gene="sdaAA"
                     /locus_tag="ACG8EF_000466"
     CDS             21578..22450
                     /gene="sdaAA"
                     /locus_tag="ACG8EF_000466"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFS4945823.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22541..23641)
                     /locus_tag="ACG8EF_000467"
                     /pseudo
     CDS             complement(22541..23641)
                     /locus_tag="ACG8EF_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(23733..24620)
                     /locus_tag="ACG8EF_000468"
     CDS             complement(23733..24620)
                     /locus_tag="ACG8EF_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4945824.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(24697..25242)
                     /gene="nusG"
                     /locus_tag="ACG8EF_000469"
     CDS             complement(24697..25242)
                     /gene="nusG"
                     /locus_tag="ACG8EF_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFS4945825.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25343..25513)
                     /gene="secE"
                     /locus_tag="ACG8EF_000470"
     CDS             complement(25343..25513)
                     /gene="secE"
                     /locus_tag="ACG8EF_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFS4945826.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25537..25689)
                     /gene="rpmG"
                     /locus_tag="ACG8EF_000471"
     CDS             complement(25537..25689)
                     /gene="rpmG"
                     /locus_tag="ACG8EF_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4945827.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            25851..26495
                     /gene="cbpA"
                     /locus_tag="ACG8EF_000472"
     CDS             25851..26495
                     /gene="cbpA"
                     /locus_tag="ACG8EF_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFS4945828.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26551..27054)
                     /locus_tag="ACG8EF_000473"
     CDS             complement(26551..27054)
                     /locus_tag="ACG8EF_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS4945829.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27067..27113)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            27651..28616
                     /locus_tag="ACG8EF_000474"
     CDS             27651..28616
                     /locus_tag="ACG8EF_000474"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4945830.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(28715..29887)
                     /locus_tag="ACG8EF_000475"
     CDS             complement(28715..29887)
                     /locus_tag="ACG8EF_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4945831.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(29874..29999)
                     /locus_tag="ACG8EF_000476"
                     /pseudo
     CDS             complement(29874..29999)
                     /locus_tag="ACG8EF_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            30107..31186
                     /locus_tag="ACG8EF_000477"
     CDS             30107..31186
                     /locus_tag="ACG8EF_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4945832.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
     gene            complement(31383..32084)
                     /locus_tag="ACG8EF_000478"
                     /pseudo
     CDS             complement(31383..32084)
                     /locus_tag="ACG8EF_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(32098..33022)
                     /locus_tag="ACG8EF_000479"
                     /pseudo
     CDS             complement(32098..33022)
                     /locus_tag="ACG8EF_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            33139..34218
                     /locus_tag="ACG8EF_000480"
     CDS             33139..34218
                     /locus_tag="ACG8EF_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS4945833.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            34215..34843
                     /locus_tag="ACG8EF_000481"
                     /pseudo
     CDS             34215..34843
                     /locus_tag="ACG8EF_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(34953..35180)
                     /locus_tag="ACG8EF_000482"
     CDS             complement(34953..35180)
                     /locus_tag="ACG8EF_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4945834.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(35283..37871)
                     /locus_tag="ACG8EF_000483"
     CDS             complement(35283..37871)
                     /locus_tag="ACG8EF_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFS4945835.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37891..38550)
                     /locus_tag="ACG8EF_000484"
     CDS             complement(37891..38550)
                     /locus_tag="ACG8EF_000484"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4945836.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(38599..39102)
                     /gene="trmL"
                     /locus_tag="ACG8EF_000485"
     CDS             complement(38599..39102)
                     /gene="trmL"
                     /locus_tag="ACG8EF_000485"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFS4945837.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(39323..40189)
                     /locus_tag="ACG8EF_000486"
     CDS             complement(39323..40189)
                     /locus_tag="ACG8EF_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS4945838.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            40298..40939
                     /locus_tag="ACG8EF_000487"
     CDS             40298..40939
                     /locus_tag="ACG8EF_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFS4945839.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(41001..42362)
                     /gene="mgtE"
                     /locus_tag="ACG8EF_000488"
     CDS             complement(41001..42362)
                     /gene="mgtE"
                     /locus_tag="ACG8EF_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFS4945840.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(42380..43279)
                     /locus_tag="ACG8EF_000489"
     CDS             complement(42380..43279)
                     /locus_tag="ACG8EF_000489"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4945841.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(43281..44078)
                     /locus_tag="ACG8EF_000490"
     CDS             complement(43281..44078)
                     /locus_tag="ACG8EF_000490"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFS4945842.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(44056..44760)
                     /locus_tag="ACG8EF_000491"
     CDS             complement(44056..44760)
                     /locus_tag="ACG8EF_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFS4945843.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44891..45466
                     /locus_tag="ACG8EF_000492"
     CDS             44891..45466
                     /locus_tag="ACG8EF_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFS4945844.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            45584..46261
                     /locus_tag="ACG8EF_000493"
     CDS             45584..46261
                     /locus_tag="ACG8EF_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFS4945845.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(46340..48148)
                     /gene="pepF"
                     /locus_tag="ACG8EF_000494"
     CDS             complement(46340..48148)
                     /gene="pepF"
                     /locus_tag="ACG8EF_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFS4945846.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(48324..49322)
                     /locus_tag="ACG8EF_000495"
     CDS             complement(48324..49322)
                     /locus_tag="ACG8EF_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFS4945847.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(49548..50201)
                     /locus_tag="ACG8EF_000496"
     CDS             complement(49548..50201)
                     /locus_tag="ACG8EF_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFS4945848.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        15081 a        10636 c         9006 g        15788 t
ORIGIN      
        1 cacttcgtac ataataaact ttaatcacat tagtctttca tacctacgat cgatctcttt
       61 gactcgcacc cataaattca taatagtttg tcggcgtctt tggacgtttc gaagatgagc
      121 tacgcattct ctttttttat agacaaaaaa gatagagacg aacatggagc ttccaaattt
      181 ggatatttga tccaattttg gaagctccat ttatttcgtg tctgtctttt tattttttcc
      241 agctttcgac taattcttgg ttctctttga tccagtcttt tgctgcttgc tgtggatctt
      301 ttccattgtt gatttccaac ataacttttt ccatatcttt ttctgaccaa tggaaattgt
      361 ctaatacttt gtaagcttct ggctggtctt ctttcaatcc ttttcgaacc atcgtatgga
      421 tctgctcttc gcttcccatt ccgttttcag gatcttctaa gtatttcaga tcatatttgg
      481 caaacatcca atgaggtgtc catcctgtaa ctacaattgg ttcgtgtttt ttgatcgctt
      541 gacctagtgc aacggtcatt gctcctgaag aagatgtctc tactttccag tctttcaagt
      601 tgtcatattt ttggatcgtg ttttcagcag ccgtaactac accagctcct ggctcgattc
      661 ctatgatttt cttacctgcc tgatcggtta actcatcgat cgaatttaca tccatgtagg
      721 ctggcactgc taaaccaact tttgctcctg tcaaattggc acctaggtct tcgacttgat
      781 ctttatattg tgcatattgt gaaccatgag ttttaggtag ccaagctgaa accatggcat
      841 ctgactcgcc atttgaaaca gatttccaca tgattgaatt atctaatggc gtcatcgtta
      901 catcatagcc catttgtttc aatacttcac cgactacatt tgttgaagca acttctgtat
      961 cccattcaac ataagataag ttgatacgtt ttgtttcttt ggcagatgaa aaactcccta
     1021 taccaatcgc acctaagatc actactacta cagcaccaat gatcagtcct tgacgttttt
     1081 tacttttctg tgttgttgct cctgcagctt ttttattgtt tggctggttc aaatgctgtg
     1141 taaaacggtc aatgatgatt gctaagataa ctaatgcaac cccgttgaca aatccattcc
     1201 cgacttgtgc tctttgcaat gctgataata cgccgcgtcc taagccgggt gcaccgatca
     1261 tagaggcaat aacgaccatt gaaagtgaca gcattgttgt ttgattgatc cctgccatga
     1321 tcgtactttt agctaaaggc aattctagtt tgaatagttt ttgacgactt gtgctcccga
     1381 atgaatcaga agcttctact aattctttag gaacttgtcg aatccctaaa ttcgtaaaac
     1441 gaacggttgg tggcagagca aagatgactg aggcaaatac ccctggaacc atcccgatac
     1501 cgaaaaatgc aactgctgga atcaaataaa cgaatcctgg cattgtctgc ataaagtcaa
     1561 ggatcggttt gataatactt tgggctttac tgctttttgc catcaagatc cctaatggta
     1621 ccccaatgat gatcgagatc acactagata aaagaacaag tgtgacagta ttcatcaaat
     1681 cattccatag accttggtta taaataaacc atagaccgat taatgtgaac aacgtaagtc
     1741 ctggacgttt cttagaaata aagaaagctg ctaccgtcaa taacaagata aacaaaggag
     1801 ctggaatcac taacagtaaa tttgtaatcc ccgacatcac tgcctgtcca atcgtttgta
     1861 aaaatgaaaa taggccagaa aaagttgtcg taaaccattc tgtgatattt tctacccagt
     1921 ttgccactgg taattgatag ttcaatagat tattcatgtt cactcacctc gcttgtttct
     1981 gctaatgctt caatcacact acctcggatg acgacacctt ttaattttcc atcctctaca
     2041 actgcaagtg gagtcggtga gtcataaata atattgaaaa tatcacttac caccatgtct
     2101 ttcccaatca ccgtcacatt ttcatctagg aagtcgatca atggacgttt ttgcttccta
     2161 gcttctaacg cttgatctgc agtgatgatc cctttcaggt gtctctttct gtcaacagcc
     2221 atcagcatgc tgacttcttc attacgcatc cttgtcaatg caacgtttgg tccatcagat
     2281 tcgatattcg tcgtcaatgc aggtaccatg atgttttgag ccgtaagcac tttggaacga
     2341 tcgacttctt ccacaaattc acgtacataa tcatttgctg gattggtcaa tatttcttca
     2401 cctgttccga tctgcatgat ttcgccgtct ttcatcaagg cgattctgtc cccaatccgt
     2461 aaagcttcat tcaaatcatg ggtaatgaaa ataatcgttt tttgaacatt ggcttgcagg
     2521 tccagtaatt cgtcttgcat ttctcgccgg atcagcggat caagagccga gaacgcttcg
     2581 tccatcagca agatctcagg atcatttgct aaagctctgg ctaggccaac ccgttgttgc
     2641 atcccaccag ataactgact aggatattgg tctttaaatg aaagcaaact agaattttct
     2701 aatgcttttt ctgctttttc ttgtcgttct tctttaggta caccacggac ttctaacccg
     2761 tattctgtat tttccaagat cgttcgatgt ggaaacagac cgaagttttg gaaaaccata
     2821 ttgattttat gacgacggac ttctcgcaat tcttctttgc ttaacttcga tacatcttct
     2881 ccatcaatat agatactgcc cgaagttggc tcaatcagac gattcaatag acggatcaat
     2941 gtggatttcc cactcccgga aagccccatg atcacgaaga tttctccttc ttttacgtca
     3001 aaattcacgt catacacacc aaccgttgca ccggttttct ttaaaatctc cgttttcggc
     3061 tggtgatcat tcatcatatc taaagcggct tgtgtctttt taccaaatat ctttgttaaa
     3121 tgttttactt ctacttttac caaacaaatt ctcctttttt atgtaaattc atttttcttt
     3181 ctgaaaaaag tgaccacact atgttaacat catgtagtca ctttgtcaaa aatgaaagaa
     3241 atttgcttgt ccaaactgaa aaaaacttgg ctaaatcttt gtaaataaaa ggtttagcca
     3301 agttaatatt tttttaatat ttcacacaat cattctgcaa gctgatgctc cgggtaaaaa
     3361 aagtttgtca tacgctgtag tgtatattca tttccaataa aatgaacgat gtcccctgcc
     3421 gtaaatttag cataggggcc aggtgaaaca agcagttctt cattatgaga aattgcgata
     3481 actgtggcgc cagtcgcctg ccagatattc aactcgctaa ttgaacggtc cagatgcatt
     3541 gctttttctg ataaagataa ttcaaacgga agtaaaggat tcatcttatg tattcgcttc
     3601 gtttgattga ccagctgatc caataattga gaaaaatgat tcagctcatc ttgttgcttc
     3661 tccacacttg ccatgatttc tgcttttgta tcttggatgg attgtacatc ttgatattgc
     3721 aataggaaat cttttgcttt ttccttcgaa gcaacaaatg ccccacttcc atgccgtact
     3781 tccatgattc caagatctac cagcacattg atagccttgc gggtagtctc aggagaagca
     3841 ttgtacgtac tggcaagtgt tgagcgggca tggatcttct cacccacttt atatcggttt
     3901 tcaacgatac gttcggctaa atcaacagca atctgctggt agcgcgggcg gacgattttt
     3961 ttctttccag acattttctt tcctctttct ttttttactt tccctcatct tactatgctc
     4021 tagcaaaata agcaaaaaag gggagaatag cctcttgcaa cgactgatca tcgcttgatc
     4081 tgttcctttt ttactgggca agctgtttga cagtcatcac aattcttacc ggattttaaa
     4141 cgagtataga caattgcgcc tgcacttcca aagattaaca cacttaataa gaatgtcgcc
     4201 atcctattcc tctccttcta atgacaatat agtataaaac tggttcttgg attttggctg
     4261 acggaacaaa acataaacga gtcctatcat cagtaaaata gcaagatacg tccctaacgc
     4321 tacctgtcca ccctccaata agtgtcccaa ctgataaatc acaaaactaa ctaaataagc
     4381 taaaccgcat tgatagccta tggcgatcca agtccatttc ttggcattca tttcccgatg
     4441 gatagcccca attgccgcaa aacaaggcgc acaaagtaaa ttgaaggcta acaatgaata
     4501 agctgctgcg ggagtatatg tgtgttgtag cacttgccag atttctctgc cattttcgga
     4561 tacttccgca tgaccaaaca aaataccaaa tgtgccaata atattttctt tagcgaccaa
     4621 tcctgtaatc gctgcgactg ttccttgcca gtttccccaa ccaagcggct gaaaaacaac
     4681 tgccaggatt cgcccgaaaa aggctaagat actttggtct tcagaaacag gttgagcata
     4741 aaaattataa gaagagctga accagatcac gatactagaa acaaagataa tcgttcctgc
     4801 ttttttaaca aaagacttgc tgcggtcata ggtttgtctt aggaccgctg accattgagg
     4861 aaaatgatat gcgggcaatt ccatgataaa aggagctgta tctccagcaa acaaacgtgt
     4921 ttttttcaag ctgattccag agaaaataat tgcagtcata ccaagaaaat aggcagaagg
     4981 agctacccaa gattgatgag gaaaaaaggc ccctgctact aaggcaataa tcggcaactt
     5041 tgcagaacaa ggcatgaagg ttgtgaccat gatcgtcaaa cgacgatctt gctcgttttc
     5101 aatcgtgcga ctggccatga cgcctggcac tccacatcct gtagcaatca gcataggaat
     5161 gaaggatttt cctgaaagac cgaatttcct gaaaagtcga tccatgacaa aagcgatcct
     5221 cgccatatat ccgcagtctt ccagaaagct caagcataaa aacaatataa tcaattgcgg
     5281 tagaaatcct aacactgctc ccacgcctgc gatgatgccg ttcaagatca gctcctgcat
     5341 ccatcccgct acttgccatt cagctaacaa tctgccaaca tgttctggta accatgtacc
     5401 aaacaatgtg tcattgatcc aatctgtccc cattgttcct actgtttgaa tagccagata
     5461 atagatcagc cacatcacaa atgcgaagat cggcaaggct aaccaacgat tcgttgcaat
     5521 ctgatcgatt ttgtcactca tcgataattg gaaagaatca tttgacacaa cacagagtgt
     5581 acatagttgg gtaataaatt cataacgttc attgaccaga atcgtctcac tatcgtcgtc
     5641 caatagtttc tctgttaact ggatcagttg ctcgatctct ttttgctgag aaactgctat
     5701 ctcgtaatcc tctcttacct tctgatctcg ttcaaaatat ttcattgcat accagcgttt
     5761 cttgccgctt tgttctccta gtacatcttc aatttcatgt aaagctgctt ctaaacgtgg
     5821 atcatatgta cagggaacag gaatagaagg ccgctttttt gcagactcct gaatcttttc
     5881 gattagtttt tcgattcctt gctttttcaa tacgctgatt ggtaaaactg gtacaccaag
     5941 accataggaa agcttttcta gattgatttt ttttccttct tttttgatga catccatcat
     6001 attcagacca atgatcatag gaatgtctaa atccaataac tgcgtagtta aataaagatt
     6061 tcgttctaaa ttggtagcat caataatgtt gatgatcagt tcaggctgtt cctgaaccaa
     6121 ataatctctt gcaatcactt cttctggtgt ataaggagac aaagaataga tgccgggtag
     6181 gtcctgaata tgaatgttcg gatttttttt caacgatcct tctttttttt ctactgttac
     6241 gcccggccaa ttccctacat attgattcga accagtcaag agattgaaca agctagtttt
     6301 cccgctgttt ggatttccga ctaacgcgat attcattctt tttcctccct ttgtatcaag
     6361 attagttcgg cctctgactt tcgtaacgtc agttcatacc ctcgcactgt cagttcgatt
     6421 ggatctccca gaggggctag ttttcggata actattttta catttcgcgt caatcccata
     6481 tccattaacc gtcgcttcac tgcgccttgt ccgtggattg ctacgacgat cccagtctct
     6541 cctgctttta attgatcaag agaagtccag ctttcagagg aagcagtctt tttattgaca
     6601 aagatttgac ctagtatttc ccgacttact gccactcgat gactttttac cagtaaaatg
     6661 gcattgttgc ctgataaatt gacgacgacg attttacttc cggcaacgat cccgatattt
     6721 tgcaaatggc gttttgtttt ttcttctgtt tcgattcgtt cgacttgata aacgaaattc
     6781 tgctttgcct gcgctaaggt ctgcatattg attcctctct tgtgaaataa atcatgtatt
     6841 aaaatcattc attatatgtt ctaattttct tacgtgttac gcaaataaat gagaatcatt
     6901 ctcacataga atactctcgg taattgagaa tgtcaattat acttgtgata ttatgagcga
     6961 atctttagta ttgatagata aaaatgagaa aaaaatattt cttcatagtt ttccacaatt
     7021 ttttctctat taaatttgct attttcccac acgatacttg ttttcagctc ttttttcaaa
     7081 aacacaaaac ttagtattcc acagtttcca caacacaata tgtagtgttt gttgtttgac
     7141 ttttttacta agagttttat actatctatt atagataata agaatgtgtt agaggaggca
     7201 ctcaccatga acgtaaaaat cttttctaaa aataattgta tgcaatgtaa gatggtcaaa
     7261 cggtttttaa cagaaaatca aattgctttt gaagaagtca atatcgatga acagcctgaa
     7321 gcagtagact ggttgaaagc acaaggattc caaagtgttc ctgtcattac ttctgatgca
     7381 gaaacagtta tcggattccg tcctgatcag ttgcgtaaac ttgccagcta acatgactaa
     7441 aagaatcaaa acgtgataca tggatttgaa gggcttgaga cagcaccagt ttttgtcgca
     7501 aacttccaaa tcctttttta aagcttaaaa acaatgcaat gatttgcaat caagcatcat
     7561 caaatacgaa atgaaggttg ggcgtacgta tgagtcttaa aaatttacaa gatgtcagtt
     7621 atttcaagct gaataatgaa atcaatcggc cagtcaatgg tcaaatccct ttgaataagg
     7681 ataaagaagc tttagttgcc ttttttgagg aaaatgtcaa accaaatacg atgacttttc
     7741 tatctattat ggaaaaaatc aattacttga tcgatcaaga atatatagaa gcagaattca
     7801 ttcaattgta ttcaccatca tttattgaaa agctatatca atttttggac gagcaacatt
     7861 tttctttcaa atcttttatg gctgcttaca aattttattc tcaatatgcg ttgaaaacca
     7921 acgacggttc cgcttatctg gaaacttatg aagatcgagt agccttcaat gcgctttact
     7981 ttgcgaacgg ggatgaagaa ttagcacttg ctcttgcaga cgaaatgatc catcaacgat
     8041 atcagccagc tacaccttct ttcttgaatg ctggacgtaa acgtcggggc gaattggtgt
     8101 cttgtttctt gactcaagtc actgacgata tgaactctat cgggcgctca atcaactctg
     8161 ctttgcaatt gtcccgtatt ggtggcggcg tagggattac tttatcaaat cttcgtgaag
     8221 ccggcgctcc tatcaaagga tatgagggtg ctgcaagtgg tgtcgttcca gtcatgaaat
     8281 tattcgaaga tagtttcagc tattctaatc aattaggtca gcgtcaagga gctggcgtgg
     8341 tttatttgaa cgttttccat ccagacatta ttgctttcct ttcagcaaaa aaagaaaatg
     8401 cagatgaaaa gattcgcgta aaaactcttt ctcttggcgt gatcgtacct gataaatttt
     8461 atgaattgac acgtaaaaac gaagatatgt atttatttag tccatatagt gtcgaaaaag
     8521 aatatggtgt cccattctct tatgtaaata ttacagctga gtatgatcat ctagttgcaa
     8581 atccaaacat tcgcaaacaa aaaatcaaag cgcgtgatct tgaaaatgaa atctcaaaat
     8641 tacaacaaga atctggctat ccttacgtaa tcaatattga tacagcgaac cgcgagaatc
     8701 caatcgatgg caagatcatc atgagtaatt tatgttcaga gattttacaa gttcaaacgc
     8761 cttctgtgat taacggcaaa caggaatatg aagtattagg aacggatatc tcatgtaact
     8821 taggctcgac aaatatcgtg aatttgatgc aaagccctaa ctttggtcaa tctgtgcgta
     8881 cgatgactcg tgcattgaca tttattacag atgcatctga tatcgatgtc gtaccaccga
     8941 tccaaaacgg aaataagctg aatcatacga ttggattagg tgccatgggc ttacacactt
     9001 atcttgcaaa agaacaaatg gaatatggct cagaggattc attagacttt acaaatattt
     9061 acttcatgct gttgaattat tggacacttg ttgaaagcaa caatatcgct cgtgaaagaa
     9121 aacaagtttt ccataatttt gaaaaatcag cttatgctga cggcacttat ttcgaaaaat
     9181 acgtaactgg agaattccag ccaaaatcag aaaaagtcaa agcattgttt gaaggcattt
     9241 ttatcccaac tgctgaagat tggaatgctt tgaaacaagc agtaatgaaa gatggactgt
     9301 atcatcaaaa tcgcttggca gtagcaccaa atggctcaat ttcatatatc aacgatacga
     9361 gtgcaagtat tcatcctatc actcgcatga tcgaagaacg ccaagagaag aaaatcggta
     9421 aaatttatta cccagcgcct tatctatcca atgagacgat tccgtattac acttctgctt
     9481 atgatatgga catgcgtaaa gtaatcgatg tttatgcaat ggcacaacaa cacgtcgatc
     9541 aaggcatgag tttgacttta ttcatgcgtt ctgaaatccc agaaggcatt tatgaatgga
     9601 agacaacaac gaagcaaacg acacgtgatt tgaatatttt aagacattac gctttccaca
     9661 aaggaatcaa atccatttat tacgtacgta cctttactga tgatgcagaa gaaatcggaa
     9721 gcaatcaatg tgaaagctgc gtgatctaat atcatagata gattcgctta ttcatatttt
     9781 aattaaagga gcattctcat gtcagaaaca tattatgaag caataaactg gaatgaaatt
     9841 gaagatatca ttgataaatc cacttgggag aagctgacag agcaattttg gctagataca
     9901 cgtatcccac tttcaaatga cctagatgat tggcgtacat tgtctcaatt ggaaaaagat
     9961 acagtaggac atgtatttgg cggtttgaca ctgctagata ctgtccaatc tgaaagtggc
    10021 atggatcagt tgcgtaaaga tgtccgcact ccccatgaag aagccgtttt gaacaatatt
    10081 caattcatgg aatccgttca tgccaaaagt tattcttcta tttttagtac actgaatacg
    10141 aaaaaagaaa tcgaagaaat ttttgactgg acaaatacga atccttatct gcaaaaaaaa
    10201 gctgagcgta tcaatgaaat ctacaaacac ggtacaccgc tagaaaagaa aattgccagc
    10261 gtctttttag aaacattctt gttctattca ggtttttata ctcctcttta ttatttagga
    10321 aacaacaaat tggctaacgt agcagagatc atcaaactaa tcatccgaga tgaatctgta
    10381 catggaacct acatcggtta caaattccaa ttaggattca acgagttacc tgaagcagaa
    10441 caagaaacgt taaaagactg gatgtacaat cttctttatg agctttacga aaacgaagaa
    10501 cgttatacag aagaattata cgatccaatc ggctggacag aagaagtcaa gacattccta
    10561 cgctataatg ccaacaaagc attgatgaac ctagggatgg atccactctt tccagatact
    10621 gccaatgatg tcaacccaat cgtaatgaac gggatttcaa caggcacaag caaccatgac
    10681 ttcttctcac aagtcggaaa cggctatttg ttaggtcatg tagaagcgat gaaagacgac
    10741 gattatttga ttggattgga ataaggattt attttaattc aaaaattttt cgaaaaataa
    10801 aaagagttta gcgtacagat attttaaatg tacactgaac tcttttttat gcttattcac
    10861 ttgtttcttc cagcactttt ggactgtcta acttttccga aacatgttcc attcttctca
    10921 ataaatagat tttccggata tttaatatta tcgtcttaat cactgcatag acaggtatgc
    10981 aaatcagcat gcctaatatt cctgcgacac ttcctgctcc aatcaatacc aagatgatcg
    11041 tcaaaggatg gatattcatt gatttcccaa ataacaacgg cttgatcaaa tttccatcta
    11101 attgttggat cactaaaatc gacaacgcca tatacaaagc ttgaaatgga gagataaaca
    11161 aaccgacaat gattgctggt accgctccaa taaaagggcc gacatacggg ataatattcg
    11221 tgattccgca aatgaccgca agcaacaagc tataaggctg atcgaatatt ctcattgcca
    11281 aaaagctcat tacaccaata atcaaagcat ctaagatcgt actgctgata taggcagata
    11341 atgtttcgtt taattctttt accgtttggc gtaactcttt acgaatcgct ttcggaaaga
    11401 agtgagacaa cgcatcaata aatttgtgcc catccttgaa cataaatagc aaaataaatg
    11461 gaacggtaaa taacaaaata aaaaattgca tcataaatga aatgattttt gagacacttg
    11521 cagataaact gactaatatg atattgaaca aattactaag agataaattt gctgatgcta
    11581 attgttcttc caaattgaaa tttttaaact cttcccgctg cgccaattca tttaaccatt
    11641 tgcttgtttc atctgcatac attggcaagc tctgggtcaa ttgaattgtc tgttccatta
    11701 actgaggcac tacattcatg acagttaaaa caactaaaat gattacgacc aaaaacgtca
    11761 ataaaaaacc gaacactctc gggatccgat gcttttcgag aaaaacaacg attggatcga
    11821 acatataata gataaaacca gcaacaagca atggtaaaaa aactgccgaa agcattttca
    11881 atattggact aaaaatattc ggcatttgta aaataaagta cacgccgatg atcgtgagta
    11941 ctagctccac tgtccagaaa aataattttg attgtttaaa acgtgattgc atacatctcc
    12001 tccaaggtcc tttgatactt ctattctatc atttaacaaa gagttacgcg ctgagatttt
    12061 catttgctta aaaaaaacaa aaaaaataaa catattttaa tgagtagacg agtacttaaa
    12121 caaaaacacg gtttggagta gtgacaaaaa ttatactgca gtcccttttt ccaaataaac
    12181 ggtgttcaaa agcttgcatc ttcgtaataa gcccagccaa acgaaaaata taaggaacta
    12241 tttttatttg gctgggctta ttacttgatg taggacatct attttacaac ctcagatcat
    12301 ttattgcgca atactgacta aatgcaactt tgtgatattt ttgtgtacgg acaactcctc
    12361 gatcacatcg gcataagtga gcccttttgg aagatcaatc gtatagatat ttttgtacac
    12421 tcggtagtca tcaacgaatt ttacgtcaaa attcacatct tcgattacga tatttttctc
    12481 ttcaaaataa tcggtaagaa aatctttggt ttcttttcta tggataaatt ggatttctaa
    12541 ctttttagtc gtaggtacat ggatcacgcg tttcactact gtgagtgcaa tagcaatccc
    12601 gataaaactg atgatcgcaa tggaatagta acccatgccg atggcgatcc ctaatccagc
    12661 aattgcccat aaagaagcgg cagtcgtaag tcccgtcacc gattgtttcg tgacgatgat
    12721 cgttcccgcg cctaagaaac cgacaccact caccacttga gcaatcagcc ttgcttcatc
    12781 tgaacgaatc gttccagcaa gctgtggata ctctaaagca tctcttaatg caccactagc
    12841 gatctcaact tggatcaatg caatgataca tgctcccata caaactaaaa tatgcgtacg
    12901 catacctgct ggacgatttt tatattgtcg ttcaaaaccg attgctccgc caaatagcat
    12961 agcgatccct aaacgtaaaa taatctctgg aattgtcaat accatatcat tcatctaaac
    13021 accctccagt ccaatatatc atgtctgaaa ggagcagaca aaaaaagtgc aatttttacc
    13081 tcttacgaaa aaaggactgt gacaaaattt ttgtcatagt cctttttatg aataaacggt
    13141 gttcaatcag ctgaccctcg gtattactca acaaacggca aaatatgggg agctattttt
    13201 cctttgtctt ctctaatact caggtcaaaa cgctgatttc acaacctctt tagcctacta
    13261 ttgaaaatta caacttactt gctgcttctt catcgatgat cacaacaaca ttgtcgtgat
    13321 tttgcaatac acttgctggc aaatgattcg ttacaggtcc ttcaatcatc cctttgattg
    13381 catcagcttt gctttcgcca aatgccatca agacgatttc tttacttttc ataattgaac
    13441 cgattcccat tgaaacagct tttgttggta catcttctgc tttatcgaag aatcgtttgt
    13501 ttgcattgat tgttgattct gttaagtcaa ctactgaagt cgttccgtca aatgaagcac
    13561 caggttcatt gaaaccgata tgtccatttt gcccgattcc taagatttgg atatcgattg
    13621 gatgtgcatc gatgatttga tcatactttt tgcatgctgc ttccagatct tctgcttttc
    13681 catctggtac aaacgtttct ttgaatggtt ttttatcaaa taattggaca tccatgaaat
    13741 gacggtagct ttgttcatct gaaccgccta gtccaacgta ctcatctaag tttacagaag
    13801 tcatttcact gaaatccaaa tcgcttgaag tcatttcttt atataaagta actggtgtgc
    13861 ttcctgttgc caatcctaat acttttgcac ctttgttcat accttctttg atgatttcaa
    13921 atgctttttt tccgccttct tcagcgtttt ttacacgaat gatttccata gtgattgctc
    13981 ctttatattt tggtatagcc caattatagc attctttttt tctttttaca ataattagta
    14041 tagaccaaat tgcatttttt tcattttctt gacaataaat aggaatccgt gttaggtttc
    14101 ataagatggt tcaatcaaac aatccatgtt gcatgaaaac caagcaagtg attcactgtg
    14161 gacgctttat gtctgcagtt tttttatgaa aaaggagtta ttatgagtat actgacatta
    14221 aacaacatta cccagcaatt tggcgacaaa atcctctatg aaggtgtcga tctgcaatta
    14281 aatgctggcg aacatcttgg gctgattgga caaaacggag ccggtaaaag tacattgatt
    14341 aaaatcatca ctggtgagat cttaccagat gacggacaga tccattggca aaaaaatatt
    14401 cacaaaggtt acctcgatca atatgtggaa gtcgatgaaa cattaacaat cgaagaattt
    14461 ctaaaaactg cgtacgcaaa agaatacgaa aaggaagaaa aaattgcttt tctttaccaa
    14521 acatatagcg agaccttcga tgattccttg ctggaaaaag ctgggaaact ccaaacggaa
    14581 ttagaccaag gagtgtttta tcaaatcgac accttagttg ctgagatgag cagcgggttg
    14641 gggatcgatg tcttaggttt agattcactg ttgaaaaatc taagtggtgg acaacgttca
    14701 aaggtgattt tagcaaaact acttttagaa aacccagatg ttctgatctt agatgaacca
    14761 accaatcact tagatgatca gcatatccaa tggctgacgc agtttcttca ggattttgac
    14821 ggtgcgtata tcgtgatttc ccatgaccaa gaatttcttg atcagatcac cacccatatc
    14881 gcagacatcg agtttggaaa gattactaag tatactggcc atctaaaaca agcattgaaa
    14941 caaaaggaac aaaatcgcga aagctatttg cgccaatatc atgctcaaca aaaacaaatc
    15001 gaaaagaccg aagcttatat tcgaaaatac aaagccggtt cccgatcaac gatggcaaaa
    15061 agccgacaaa aacaattaga taaaatcgaa cggctgacgc ctccagcttc agcaagtaaa
    15121 gctgtctttg attttccatt ctcgcctatt gtcacgacat tagcagtgga aacaactgat
    15181 ttagtcatcg gatatgatcg accgctactt gcacccatca atttgaccat ccgctttggt
    15241 gaaaatattg ccattcgagg cttcaacggg attggaaaat ctactttatt aaaaacactg
    15301 atcggtgaga ttgaaaaaat aagtggcgat ttccacttcc cagataacac aaaaatcaat
    15361 tacttttcac aagatctgta ctgggaaaat ccattagaaa caccactaca atatttaagc
    15421 agtcaatttc caaaagcgac tatcaaagaa ttacgtcgcc aactagctaa agcagggtta
    15481 gttaatcaat tagcaagtga acctctcact acattaagtg gtggggaaca aactaaggtg
    15541 aaacttgcac agttgacaat gaaccaaggt aatttactga ttctcgatga accgacgaac
    15601 catatcgatc aagaaacgaa agaaagctta caagacggga tacagaaatt tccaggaaca
    15661 gtaatcatcg tctctcatga acaagaattt tatcaagatc ttgtgcaacg agtcattgag
    15721 attgaaccaa aataaacgtt tgattgaata gttgaaaaaa aggagctgtg acaaaagttt
    15781 tgtcacaact ccttcttctg aataaatggt ggccaattag ctgaccttcg gtattagctc
    15841 aaaaaacggc aaaatatgag gagctatttt tatttggcta ttcttattac ttgatgcaga
    15901 acagcttttc acaacctctt ttcgtctatt ttttattttc gagatttctc tttcctttaa
    15961 aaacaactgt ttgaaagaag aacgcacatc cttgaaaaac aagcccgcta accgttacac
    16021 ctaaccagcc atactgctgc cacattgtcg tccctagcaa tgaaccagcc gcacctccaa
    16081 taaagtacat aaacataaat acagtattat ttcggttgct tgctgcttca cctagccctt
    16141 gtactcgtgt ttgattggcg acttgaccaa attgattccc gacgtctaat aaaataatag
    16201 ctaaaagcaa aatggccaaa tggcttccgc caaccaataa agcaataaag cttattgttt
    16261 gcataacaag tcccatatac acgatttttc tttcagaata agaatctgcc agtcgtccca
    16321 cgataggtgc agcaactgct ccagacaaac caaaaatcgc taatatccct gcttcgaatg
    16381 ttccccaatt gtaggcggga ctgctgatat aaaaaatcaa cgtagaccaa aagatcgaaa
    16441 atgttccaaa catgaaaaat ccactgactg ccgcttctct taatagacgt tgagaagcaa
    16501 ctaaatgcgg caaactttta agtgaatcaa ggtatgaaac agctgtacta ctttgctgga
    16561 cagaacgcgg cattttccag taaagcagaa gtgtaagaat tccgactgca cataaagcaa
    16621 ataaatagat catccgccaa gaaaaaagac tggcaacaat accagatacc gtccgagaaa
    16681 gcaaaatccc cacgagtaaa ccgctaagca atgtacccat gaccttccct cttgcttttg
    16741 gtcccgctag taccgcaccg taaggaatga tgatttgcgg cacgatcgaa agtaatccta
    16801 ttaaaaaaga agcgcatgca aataacgtaa acgaaggcgc aaaaaaagcg gccaacagag
    16861 agacggcaga taacgctgcc attcgaatga tcaattttgg acgatccacc acgtccccta
    16921 aaggaactaa aaacagtaac cctaacgcat agcctaattg ggtcaacatc gtcaatatcc
    16981 cgactgcact tgtgctaaca gataaactag ctgctacttt tgttccaatc ggctggatgt
    17041 aatacatatt ggcaacaaca acgccacaag tgattgataa tagtaaaata agcgaagttg
    17101 ttaaaggttt tttttcatta ctcttcattg atttccccca tttcctatct tagtttacaa
    17161 cttatggaaa agattggaaa tagaaaaata caactattcg ccacacttat tatttgtgct
    17221 tttgttttcc ttgttttttc ttgcgatctt tataaatgaa gacaactaac cctactagca
    17281 cagtaacact tgcaaccaac gtgactgcag ttttgttata tccgtgaaac agagcttctc
    17341 caccacacgc atacaacaca gacgaaggga tgactccaat cacgatcgac aatatgattt
    17401 gctgtagagg caatttcatc gcatctgcag caaaattgac catagaagat ggaataaccg
    17461 gtatcatata gcctaacatt acacctattt ttggatgttt catttgtcgg attgcttgca
    17521 cccaatgatt cgtttcggtt ttcttatcta aaaattttaa gcgatgcata aggaaaattg
    17581 cagtgatgtt accaagagca ttacccaaaa tgttgatgat cgcacctgtc agtggtccaa
    17641 agctcaaacc agcaatgact ccaactactg aagtaggaat acctggaaca gcacacatca
    17701 ccgctgtcaa tacgatcaag agtatcgatg caaaagtccc atgcgaacga atatgtgcga
    17761 gtagctctgc cttactcgct ctcggattta aaaataattg gatgtctgtc tgatattcta
    17821 aatacaaccg atagcccact accagtatca gaatgattcc caacgagatc aatatcaatc
    17881 ggattatttt ctttttcata tttcctcctg aaaaaaatct atattcatta aataatagtt
    17941 taacataaaa acaaaaaagc accgaaatcc aacataggat ctcagtgctt ttaaatagta
    18001 aaaattattt aacagttaca gatgcgccaa cttcttcaag tttagctttc aattcttcag
    18061 cttcttcttt agatacgcct tctttaactg gtgcaggagc gccatcaact acagctttag
    18121 cttctttcaa gcctaaacca gttgcttcac gaactgcttt gataacttta actttttggt
    18181 ctccagctgc agttaattct acagtgaatt ctgtttgttc ttcagcagca gcagcgccac
    18241 cagcagcagc tacagctaca ggagcagcag cagatacgcc aaattcttct tcaatagctt
    18301 taactaggtc gttaagttct aggatagttg cttcttttaa ctcagcaacg atgttttcaa
    18361 tgttcaatgc cattataatt tcctccgttt aattgttctt ttttatttaa tgctttatgc
    18421 ggcaattaag ctgcatctcc atcttctttg tctgccactg ctttgacagc gtaagccacg
    18481 ttgcggactg gcgcttgtag tacagatagc aacatagata gaagtccttc gcgatttggc
    18541 aattttgcca atgctgtgat ttcttccaat gaagagactt taccttcgat gataccgcct
    18601 ttgatttcta atgcttttgc ttctttagca aattcgtcca tgattttcgc tggtgcgact
    18661 acatcttcgt tactgaatgc aacagctgta ggtccagtga atacttcgtc catgccttca
    18721 aggccagctt ttttagctgc acgtgaaaga atagaatttt tgataacttt catttctacg
    18781 ttagcatcac gaagttgttt acgtaaacgt gttacttcat caactgttaa tccgcggtag
    18841 tcaacgacta ctactgatgc agctgctgta aatttttcgc ttacttcatc aacgattgct
    18901 gctttttttg cgataattgc ttcactcatt tttgagtttc acctcctgaa ttttgatgga
    18961 agagtttttt gcactgaaaa ggcaagtcac ttttatagtt actttgctca accagtatta
    19021 ttcatcacct attcacttcg ttcatagtga ttctactcgc tttgctcgaa aggaaactcc
    19081 tttcgctttg ctcaaccagt attgttcatc atctcttcac ttcgttcata gtgattcaca
    19141 ataaaaaact ctatgccacc gcagacatag agggactatt gaatatattc aataaacgtc
    19201 ctcggtagga aattaaggct tgatgccacc tactgtcttc ggtacaagtt aattattaac
    19261 ctcaactagc ttagcatgct aaactagttg agtcaagaat tatttttcag ttggatcaat
    19321 caacattcta atatttaatt agaaagaagc ttggtcaaca tggataccag gtccaaatgt
    19381 tgttgtgatc gttacatttt tcatgtattg acctttagct gctgatggtt tagcttttag
    19441 caatacatca tggattgctg caaagttttc aactaatttt tcatcgtcaa atgaaacttt
    19501 tccgattggt acatggatat ttccagcttt gtccacacgg taagttactt taccagcttt
    19561 cacttcgttt actgcttttg ttacgtccat tgtaactgta ccagttttag ggtttggcat
    19621 taagcctttt ggtcctaata ctcgtcctag acgacctaca gtagccatca tatcaggtgt
    19681 tgcaacaact acatcaaagt caaaccatcc gttttggatt ttttgaacca tgtcgtcgtc
    19741 acctacgaaa tctgcaccag ctgcttcagc ttcttttgct ttgtctcctt tagcaaaaac
    19801 taaaacagtt tgtgttttac cagttccgtt tggtaataca acagcaccac ggatttgttg
    19861 gtctgctttt ttagggtcta cgttcaaacg gtatgcaact tcaactgttg catcaaattt
    19921 tgcaatgttt gtttcttttg ctaaagctac tgcttcggca acaggataaa ctttacttga
    19981 atcaactttt tttaatgcat cttgcatttt tttgctcttt ttagccattt tggttcctcc
    20041 ttgattgtgg ttataacggt tgtacctccc acgtgttcta tatctttcaa tatagagcca
    20101 actgagaagc tgctgtctta gggtgtatag attattctac agtgatcccc atgcttcgtg
    20161 cagttccttc aaccatgcgc attgctgctt caacgttagc tgcgtttagg tcttccattt
    20221 ttaattcagc gatttctctt acttgatcgc tagaaacttt cgcaacttta tttttgtttg
    20281 gttcgcctga acctttttcg attttagctg cttttttcaa tagaacagca gctggcggtg
    20341 tttttgtaac gaatgtgaat gaacggtctt cgtatacaga aataacaacc ggaatgatca
    20401 aacctgcttg atcagctgta cgagcgttga attcttttgt gaatcccatg atattgatac
    20461 ccgcttgacc tagtgcagga cctactggtg gagctggagt tgctttacct gcaggaattt
    20521 gcaatttaac gattttttct actttctttg ccacgagaca tacctcctta agtccgtgat
    20581 gtggttaatt ggagatgcat atttctcctc ccacgtttta tctgatctga aaaaatccag
    20641 acaaataatg tgcaaaacaa tgcacactgg tacagtatag caaatttatt tgttttttca
    20701 agattttttt ctgctatttt tacattttgc ttgaataaac gctaaattta ttctaaaaaa
    20761 acaacaattt ttcgttctca tttcttcttt tatgttacac ttgcgctagc atggttcaac
    20821 agatgaaagc aaacaataac acggtttttt agacccttgt acaaagattc ctacataaca
    20881 aaatgaggtg gagaaaatga aagatttaag gtatagaagt gtctttgata ttattggacc
    20941 tgtgatgatt ggtccaagta gctctcacac tgccggagct gctagaatcg ggaaaatcat
    21001 acgtagtata tttggcgaac agcctgattc tgtagatatt tatctatatg aatcatttgc
    21061 aaaaacctat cgggggcacg gaacagatat tgcattagtt ggcggcctgt tagggatgga
    21121 acccgatgat gaacgtttag ctgattcttt gaaattggca tatgaagccg gtatggaagt
    21181 cttgtttgtt cctaagagtg aaaaagccga acatccaaac tctgtaaaga tgcttctaaa
    21241 aaaaggaaca cgaaagcttt cagtcactgg catttcgatc ggcggtggca atatccagat
    21301 ttctgaattg aatggattca aaatttcttt aagcatgggt actcctacct tgatcgttgt
    21361 ccaccaagat gttccaggta tgattgctaa agtgactagt atactttctg agaccaatac
    21421 gaatatcggc actatgacag tgacacggga atcaaaaggt gaaaatgcca ttatgataat
    21481 tgaagtagat gatcctcaag tagaagaaac ggtaaaaaga ctcaaacagc tgccaaatat
    21541 cgacagcgta aattatttcg attaaggagc taaaaaaatg tttttatcga ttgaagaatt
    21601 agtagatcaa gcaaaagctt atccaagtgt agcagaattg atgattgcag ttgaaatgga
    21661 aatgagtggc cgttcccgta gtcacatcat cgagatcatg gaaaaaaacc tctcagtaat
    21721 ggaacaatca atcaacgagg gaaaagaagg tgttacctcc gtcacaggaa ttaccggtgg
    21781 tgatgcaaaa aaattagatg attatttaaa tgaggcagac tttttaagtg gagaaacaat
    21841 cttaaaagct gtacgcaatg ctgtagctgt taatgaagtc aatgcaaaaa tggggttgat
    21901 ttgtgctaca ccgactgccg gaagcgcggg tgttgttcct ggtgtgctga tggctgtccg
    21961 agatcggctt gatttgacac atgaacagca attggaattc ttatttaccg ctggcgcttt
    22021 cgggttagtc atcgcaaaca acgcttctat cagtggtgca gaagggggat gtcaagcaga
    22081 agttggttct gcaagtgcaa tggcagcagc agctttggtt tgtgcaaaaa atgggactcc
    22141 tcatcaagca gcacaagcag tagccattac attgaaaaat atgatgggac tgatttgcga
    22201 tcccgttgca ggcttagttg aagtcccgtg tgtcaaacgt aatgctttag gctcttctca
    22261 agcttttatc tctgctgata tggcattagc gggtatcgaa agtgtcattc cgccggatga
    22321 agtcgttgct gcaatgtacc aagtcggacg tcaaatgccg tcgattttca aagaaacagc
    22381 agaaggtggc ttagctgtta cgccaactgc taaacgactt gcggaagaga ttttaaataa
    22441 gagggcgtga taaagctcct taatttcgag tattaagaga ttagttatct tcataacaaa
    22501 aaacgaagag aggactaatt gcccttctct tcgtttttta ttaatccatc aatttcgtat
    22561 tttttacttc atcgatcgtt tttgttcctg caagttgcat cgtgatcgac aattctttgt
    22621 tcaggtgttc aaaaacagat gttactcctt ctgccccacc tagattcaac ccataaatca
    22681 ctggtcggcc aatcgcaact aagtcagcac cacttgccag agctttgaag acatgctctc
    22741 ctcgacggac accagagtcg aaaatcactg gtacacgttt gttgactact tcggcaattt
    22801 ttggcaatac ttcaaaggac gcaggacctc catctagttg acgtccacca tgattggata
    22861 cccagatacc atctgcccct gcagagatag cgattactgc atcttctggc gattgtattc
    22921 cttttacgat aactggaaga tgggtgattt ctttgattgt tttgatgtca tctggtgtca
    22981 agccttgttt tgcagctgca taaatttctg cgattccttt tccttcgcca ttaccacttg
    23041 cactttgttc gctatacgca gctaagttag gcattggtaa tggaaattgg aattgattga
    23101 tgacgtcttc ttcacggtaa ccaccgagcg ttgaatctgc cgttaaaatg atagctttag
    23161 cccctgcttc tactgctttg ttcaaaatga actcattgaa tctgtcgtct ttactcatat
    23221 ataattggaa aaactgcggt gcgcctggcg ctgcatcggc tgcgtctttg attgtcgtat
    23281 ttgcataagt gctgatggaa aagattgatc cagcagcagc tactccttta gctgtatcag
    23341 cttccccttt ttcatgtgcc aatccttggg ccgctgatgg cgcttggata atcggtgtat
    23401 ctaattcaat tccgaaaaca gatgtatgca aatcggctga gtcgattccg cgtaatatcc
    23461 gtggcatgat tttctttgta ttaaaagaag tcgtattttc tttcatcgtc cactcatctt
    23521 cagaaccacc gcgaatatag ccaaaagctc ctgcttccat gcgctctttt acacgacctt
    23581 ctagttaggc aatgtttaca atatcgattg gatgttcttc attacttgta tgataagtca
    23641 tagtagatcc ttctttcatc ttcttgatta catataaatc atactaaact tacaaaaagt
    23701 aagcaaacat ttactctcag atacttttat cgctattctt tccaaataaa tcgacttaca
    23761 agctgatcta cttcttggtt ttcatgaagc atactatgag aggcattttc acctgtcact
    23821 actttttcct ctatttcatt tccgcgttgc ttcaacaacg catagaccgc taatgcactg
    23881 tttaacggta ctgttccatc actcaagtct gtttcatcta gttgaccagc aatcaagaaa
    23941 aatcgagtgg ttataggaat attaccgatc atttgctggt aatcttgata gcggttagaa
    24001 acgacagcag gcccctttgc taactcatct gtcagagact gcgcactatc atcgacaaaa
    24061 tcattaaaag gcgcgccaat tgctgttaat gtctgaactt gtggcaatga cgtatcctgt
    24121 ccatacgttc ctaaatagcg tagtgcacta actcctccca tggaatgccc cactatgttg
    24181 actttatcta tgccgtagtt ttctttcaga tacaaaagac aagttttgat ccattctgct
    24241 tgattccact cgttattttt attatcttca aacaatactt ggaccatcgg attgtctgct
    24301 tttccagaca aatctcctgt tgcttgtatt tgtccatctg ttcctactgt taaaaccaat
    24361 tcttttactg cttgctgatt tttttccaaa cgccgcaaca tccctttaaa ggaattttct
    24421 gtcccgctgt agccgtggaa aaaaagtgtt ggaatagtgg ttttttctat cactttactg
    24481 gattctgccg ttttttcttt atcagaaact tcagtagtat ttatcttcgt cgtctggcaa
    24541 ccacttaacg caaacaaact gataaggact aaagctgctg cagttgcacg tttgcataaa
    24601 tttttgattg gttctgtcat acacctcttc ctttccatat agaaaaaaga aagacttgaa
    24661 aggccaatca tatgatcgac atttcaagtc tctttattaa tcgattttat ctacttgatc
    24721 gaaatcaagt tctgtactgg tttcacgtcc aaacatatcg atgttgactt tcaatttttc
    24781 tttttctccg tcaacttctg ttacttgtcc ttctaatcca gcaaatgcgc cttcgatgat
    24841 tttcacgact tcgccaactt ctacttctag atcattttgt cgcaggctca taccgataga
    24901 acgcaagata tgattgactt cttctggaag taatggtgct ggtttgcttc cggctccgtg
    24961 tgaaccaacg aatcctgtca caccaggtgt gttgcggaca acataccatg aagcgtctgt
    25021 catgatcatt tcaacaagta cgtatcctgg gaatgtttta tgaacgattt ctttttcttt
    25081 cccatttttg acttcagttt ctttttcttc aggaacaacg acacggaaaa taaagtcggc
    25141 cattttcata ctttgcgcac gagattcgat atttgctttt actttgtttt catatcctga
    25201 atatgtatgc aagacatacc agtttttttc aaaagattcc attgaaggct cctcatttca
    25261 ttgtaaaata aaaaaacctc agtacacccg aagtttttct ttcgcttcat tatagcacta
    25321 aaagtacgtt gtggctagcg agttatttta aaatccagcc aaataagctt tggatggctg
    25381 tatccattac gtaaaacatc aatccgaaaa taatagaagt ttcgatgact actaacgtat
    25441 ctttacggag ttgttttctg cttggccaag ttacttgttt gatttcatct tttacgcttt
    25501 ttagaaattt catgagacaa cctcccttgc tactgtttat ttcgtttctt tatgtaatgt
    25561 atattgatta caatatttac aaaacttatt gatttccaaa cgttcgccgc tttttccttc
    25621 actgactgat tttgtgtagt tgcgagaccc acaaacggaa caagcgagtg ctgctttttt
    25681 ctttgccatt tacttctgcc cccttgctga gaatcagact tatacttcat aacagtatca
    25741 cgtaactttt tacttgtcaa tttgtgaaat aaactatcta ttgttttgga taaaattatg
    25801 atatgattaa tttgaaatta agctaaaatg agacaattgg aggtatagcg atgctattaa
    25861 aaacacttgt atataagaaa aaagatctga caaccgttac agagactagt acattagaag
    25921 aagcattaaa aattttagaa acttccggat accgttgtgt gccgatctta gattcttcag
    25981 gtaatatttt ccgtggaaac atttacaaaa tgcatatcta tcgtcataaa gcaaatggcg
    26041 gtgatatgac gcttcctgtc actcatcttt tgaaaaacgc tacaaagttc atttacctgg
    26101 atacttcttt cttcaaagta ttctttacga tcaaagagct tccttatatt gctgtccttg
    26161 acagtgaaaa tcatttttat ggtattttga cacatagtac gttgcttaat atgctctctc
    26221 aatcttggag cgtagaacaa ggaagctatg tactgacaat tgcttccaca ggaaagcaag
    26281 gagatttggc tgctattgca aaaatcattt caaaacacag cagcattgcc agctgtatta
    26341 ctcttgatat cgaaaaagac gaatacattc gacgtacatt gatcacgcta ccggctaaca
    26401 ctgaagcgaa agtatgtgaa acgatcgtca accacttaga gaaaaagaat tataaagtgg
    26461 tagaaattga aaatttgcaa aaccaagaag cataatcaaa aagcgaaaaa agaaaacagc
    26521 taatctgctt tcttttttcg ctttttataa ttagacacgc ttttcaaaat caagcgcgcg
    26581 atctaaataa tacatcaatg gtgctgaaac agccatgacg ataaactcac ttaatgcaag
    26641 cgtgccatag gttgtccaaa atgcttggcc acctgttgga actaacatat aagcaatcag
    26701 tcccatgctg atggtaaaga agatcgtgtt caatactaaa cgcatcttta catttttcac
    26761 tttgttttgc aataaagctg tcaggcctaa agaaagcaat gtttggccgc caccaaaaac
    26821 cacatccatg ataccaaaac caaagaaggc attatagacg atgaccccgc ctaaaactcc
    26881 ccaaagcaat ttacggttaa agacaactaa atgattcaaa ctttctgaaa tacgaaactg
    26941 gatcggccct gaggatacag gcgcaactaa aatagttaac gctagataaa gagccatgat
    27001 gatgccattt acaacgatta cggataaccg ttgtctgctt ttcgtttttt gcattttctc
    27061 tcctcttttc tcctagtttt tttaacgcgg gatggttgat gaaccgcggt tatgtgaaac
    27121 aaacaataat tataacagac ttttcattta tttcaatccg tcttatctgc ttttaaaaaa
    27181 gcaaaagctt aaacaatgta aacgtttgct aaaaatcgtg ggatatggta taaagtaagt
    27241 gtaaggggtc ataagaattc ccagtaaagc agacgaagta ggatctgcaa gaacagaatt
    27301 gttgtgaaac ttaaataaat aaaaaaggat gtgggaattt gagcaacctc ttactagaga
    27361 acggtctagc aagcagctga agatctaagt ggacatgccg aaccttggaa cttctcctta
    27421 cgtaagtaat ggatcatgcg cttgctaggc ttttttgtgt ttttcggtct tcggaccttt
    27481 ttcattttcc tagacttctg ctataataac caagaattaa ttttgtaaca gatttttttg
    27541 aaaaaacaag tttctttcat tcaaaaaata gaaaaagctt gctaattatt ggttaaccag
    27601 caaacaggag tattaaaata tcttgtttta ttgaatagca ggaggaaatc atgaaattcc
    27661 attatcatat attgatcaat ccatcggcag gcagcggaaa tggctataaa gtagcagaaa
    27721 gaattttacc ggtattaaaa aataagcata ttgactatac acttcattat agtgaataca
    27781 aaggacacga tgctgagatt gctgaaactt tggcaaaaga aacattgatt ccttggatag
    27841 aagaagaaca gaaaacaatc gatactttcc cccttttgat catcgtagga ggtgacggca
    27901 ccttacatca ggtacttgat actttctatc agatggaagt agaattccca gttgcttata
    27961 ttccagcagg ttctggagat gattttgctc gaggggcagg ccttccaaaa aatcccaaaa
    28021 aaggacttca gctgatttta gcagctcaat caccagaaaa agtccacata atggcttatg
    28081 aagaaaaaat aagtgaaaaa aaaggaatag ctgttaataa tttcggtata ggattagatg
    28141 cagctatcgt ccatgcgaca aatcattctt ccacaaaaaa gcgattgaac aaatacaact
    28201 taggatcatt ttcgtattta ttctctattc tccgtgcttt attcactcaa aaaggatttc
    28261 caattctggt agatgcagga ggaaaacaat taagtttttc aaacgcattt ttatgtacag
    28321 caacgaagca tccttttttt ggcggaggaa tcgcgattgt tccaactgct gatgcgtcta
    28381 aaccagtcat tgattttgtc gtggtcgaac gaatcaattt ctttaaaatc ttgtggctca
    28441 ttcttttact tatacagaaa aaacaattga aatcgaaata tttccatcac tacacaacaa
    28501 acagattacg tatcgtttca acaacaccgc aacatggtca agaagatgga gaagaaatgg
    28561 aaaaacgacc ttttgatatc acaataacca caactaaaca gttgttttgg tgttaataaa
    28621 agaggttttg tcagaagcta catctggaac accgtttatt cggaaaaaag gactgtgaca
    28681 cgacttttgt cacagtcctt tttttactat gcagttaatt ctttgcccaa ttgggttgcc
    28741 atcgatagtt tctttcttta cccaaaataa attggatcat tgaaaacacc gcagcaaaag
    28801 caactaagac acaaccattg aaccaaaaag attctgcaaa tgtttgattg taactgagtg
    28861 tttcactgat caagttatta ttttgaacaa accaatatgt tggtgtgaat gccgctattt
    28921 tgctaaccat ttctggcaag tattctgctg gtacaaacac cccaccgata aagcaactgc
    28981 ctgtgataaa aatatctttg atcccaccgc tggtttctga gttttttacc aatgaagtga
    29041 tacaagccga aaagctaatg atcggcaata aaaacaagaa agtatttaaa atatgatacc
    29101 ataccgttat atcccacgag gtgtggatca ctaacagaac cattagaagt aagaagccac
    29161 tgaacaacag cagcgagtaa ctaatcaaac taaaggttac tttgcgattc aagtttcgtt
    29221 tggtaatggg cgcactctgg tttcgtttac tgatctcttt gcgattaaat gctagattga
    29281 tgacgctgaa accgctaaaa atggatagaa acaacccata agacaagaaa ttaaaggttg
    29341 tgcccgtcaa cagctttttc atccgctgac tatatcccgc acttagctca acttctccag
    29401 taaccgagag tgccatttgt gtctgttcca tagctgcatc gattgtttta tcttcttctt
    29461 gaaacagaat cagtgtttgc aaaaaggtgt tcacttgctg tgtaacaact gttttagaaa
    29521 aggcatctgg gcgactctgt atcttgatcg tcggcatctt tcctacttcg acttgttcgg
    29581 taaagtctgc aggcagatgt aagataatgt ttacacgatt aaaataaaga gcatcatcga
    29641 tttctttttg tgaggttcca tccaatgtaa cgactgtttc cttgtcattc aaatatttac
    29701 gaaggccatc gactacaggc gactcttctt cactaatcaa ggcgattttc gcatcttgca
    29761 gagaggtatt ttcaacatca atattttgcg caaaaaagaa tgtggcaaca acagtgataa
    29821 ttacacctat cagtaaattc gatttatttg ctttaagcaa tgtccaaatc gttttataaa
    29881 gcgtcataac gcacccctct ttccatccaa gcatttccta acgcaaaaag gaaaataaat
    29941 ccgatcaacc atagaagatt ggtataaaaa ctagccaaat cagtgtagaa ataaagttta
    30001 ggcggatttt ataatgaact tagccattta aaataaaaaa aacgccacgt gaattccatg
    30061 ttatattgaa gttaccacaa ctctcaatag acaaggatga attcacatga cgcaaaccca
    30121 tgataacaca gtttcacgca agggaaaaca tctttcctac tcagaacgtt gccaaatcgc
    30181 tatcctgaaa aaggaaggct attccaaccg tcaggttgcc aaagtgctgg gacgtgttcc
    30241 gcaaaccata aacaatgaga ttgatcgcgg aaccatcacc cagttaaaac gccaaacgca
    30301 aaatggaaaa gtgtatgaca cctactattc tgtttatgac gctgatgcag gacaggcctt
    30361 ctatgaaaag caacgactga actgtggcag acgccccaaa tgggcgaaca caaatgcctt
    30421 cattgattgg gcagatgaca aaatgttaaa tgaaaaatgg tcgccggacg tcgtggtcgg
    30481 atttgccaga aaacacgagc tgttcgattg tgccatcatt cctggcacaa ccgctcttta
    30541 tcaatggatt gaccgcggaa tcatgcgaac aaagaacatg gacttgcttg aaaaactgtc
    30601 ccgtaaaatc aaagcggtcc gcccaggaca ccgtcccaac aagcgtgttc tcgggacgtc
    30661 aattgaagaa cgccctaaag aagttgaaac acgggaaacg ttcggtcatt gggaaatcga
    30721 cacggtagtg ggaaacaaga ccaagtcgga tgccgtgctg ctgacgctgg ctgaacggca
    30781 gacacgcttt gaagtcatcc taaagttgaa cgggaaggac accgaatcgg tggaccaagc
    30841 ggttcaacag ctaagagaac gggctggcgc taacttcgat caattattta agaccgttac
    30901 atccgacaat ggctcggagt tttctgggct acacgatgct ctgcaagatg tagctgacgt
    30961 ctatttcagc cacccctatg cttcttggga gcgtgggacg agcgaaaacc agcacaaatt
    31021 aattcgccgt ttcgtcccca agggaagccc gattagtgag gtgagcgaga accagatttt
    31081 gcggatccag cgttggatga acgattaccc gcggaaaatt ttgggttatg cgacaccaca
    31141 tgatacattc gtccaggcat tcaagcaaga acggttggtg gcttgaccca ttattccttg
    31201 ataacgatcc tcactcggga catggcttca tgtctggaaa gctggcctgg ggccagaatc
    31261 gtctgaagac tatcatacct cagttcatta tcaaggcctc actgatttag aataacgagg
    31321 cagttacggt atcaaacaaa tgagttacct gtagtggcta acttaaactt gaaatttacg
    31381 gacgtaaagc ttgctgccaa attagcgatt gaaactaacg attgctttct aggaatcaag
    31441 gacaagcgaa taccatttga cgattgattt gcttgctgat cctgtacatt gcggaccccc
    31501 cacatcgtcc cgtagagaat cgccattcca atcagagtga agaaataaaa acttttgaaa
    31561 ctcatattac gacctgcatg aacttcttga aaagatactt cttgttcaaa aacagtcatg
    31621 atgggcgcga gatcaacgtt ctccacttct gccactttac tcgaaattat accaatacgc
    31681 tgtaaaaatt gatccatgac gtttttcatc aacgtctgac tcattcctgc ttgacccaca
    31741 tggagcgaaa cagatgaacc atcaatgaga taaaaaccag ccaaatcatc tgcatttagc
    31801 ttttcattcg cttctacttc cgatagttcc tgtacttcaa ataatggttt atcattgact
    31861 tcgacttgtt ctaagactgt ggtaaaattt tccgaaagcg ctgtttgatc cgctttgatt
    31921 acaattccag tattaatcgt ttcgagattg ccaccttgat caatctcgcc aaaggccgcc
    31981 ataaacatca atcctaaaag aattggaaag gccaatgtcc aaaacaacat ggatttattt
    32041 cgcagcagta tttttgtgcg atatttataa aaatgccaaa acattatttc ccctcctcta
    32101 gtctctcaaa tccttgccag taatctctaa aaagacatca ttcaaggacg cttctttggt
    32161 tgctattttt ccgtaactga tcttttcttc ttgtaagatt gtaagaatcg gcaacaatac
    32221 tgtatttagt tcagtagttg ttacttggag ctgacctttt ttataagcga ttccatcaga
    32281 agctattgtt cgctgacaac acgttttctg ggagtaatgg gatgtcgatc gtcaaacgat
    32341 ccgccgtccg taccatatct tttagagatt ctttggttcc tttagcaatc acctgtcctt
    32401 gatccatgat cacgatctgg tcgcaaagaa tttctacttc ttccatgtaa tgtgttgtat
    32461 agacaatggt tgctccttgt cgattcagct ccttgatact ttctaaaatt ttattccgac
    32521 tttgggggtc tacagctact gtcggctcat ctaaaaaaat taaccgcggc ttgtgggcaa
    32581 tcccacatgc gatgttcaac cgacgtttca agccaccact aagttctcct ggacgaaacg
    32641 aaacatattt ttctaatccc actaattgaa tcacctcttc aatcaaggct ttccttttct
    32701 ctttttcgga aacatataaa ccgcaaaaat aatcgatatt ttcttgcaca cttagttcgt
    32761 caaagaccgc aacgtcttga ggaacgaccc caatcattgc cttgacagca taattttcag
    32821 gagtcattgt tgtcccgaac aatttaatgg ttcctttgtc ataatttaac aaagaaagca
    32881 tacagttgat ggcagtcgat ttcccactgc cgtttggtcc taacagacct aagacttctc
    32941 cttcttgtac ttgcagatca aaatgattca atgctacaaa atctccatag cgtttgacta
    33001 atttatcaat ttcgataatc atcatgaaaa actccttcca aatttcttgt tgatctgact
    33061 ataataaagt gcaagaaaag aaactagtga cgattggcta aaccacacat gacatttgtc
    33121 acaatgaagg agtctgctat gcataaatct cggctactca acgaaactag ctttctactg
    33181 gttatctcat tgcttttgct ggtaagtcaa ggattcgttg ctatgcaagt tatctattta
    33241 ttgatcgccg ttttattcac tggtgtcagt tatttactgc ctacaaagcc aaattggatt
    33301 ttccaagtta ttctatttct tttatgtgcc tttttacctg atcttgtcct gtttctgcca
    33361 gcgacgatgc gagtgatcgc ccctgaagaa agccaccttg attacttgtt tttgttaggt
    33421 tttttgttga tttcgacggt tggtaatcat ccccccatca aggtcgcctt tgtcgtaatt
    33481 ttgctgtttt tttcctttta cttacgaaaa aaagatcaac gaacgaccga tcttgagaaa
    33541 aagtacattg tctccaaaga tactcattgg gaaaaacaac aagagttatc ggaacaaaat
    33601 aaagcattgg ccctgtcaca agaaacacaa ttagccttgc aaattgtgga agaacggaac
    33661 cgaattgccc gagacatcca tgacaatgtc ggtcatctgc tgtcaagtgc gattttacaa
    33721 attggtgcgc ttgaaatgat caatcaagaa ccaaaactaa aagaaccatt agaaaattta
    33781 agtgccaccg ttcacactgg gatggatcgg attcgtcaaa gtgtccatga cctccatgag
    33841 acatcgatct cttttcaaca ctccttagag tttctgatcg ctgattttcc ttcttctgtt
    33901 acgattgaag gaaacttgtt tgaaggattg aatgaaacgc agcaaaactg cttcttaatg
    33961 gtcatcaaag aagccttaac caacagtatc aaacatagtc atgccaaaac cgttgtttta
    34021 cattttcgaa cgttgccggc cttttatcgc ttgcaaatca cgaatgatgg caccagccca
    34081 caaaaaaatt ctgctcccgg tattggtttg aaaacgatgc gtcaacgtgt tcaagaacaa
    34141 aagggacaag tccatatttt ccaagaaaac cagcaatttc agttaacgat cattttacca
    34201 agggagggac accaatgata acagtagtca ttattgagat gatccatttg tgacgaaatc
    34261 gttacaaacc attttagaaa gcacgaaaga gatccgtgtg ttaggcattg gtcattgtgc
    34321 caaggatgcg ctagtcttat acgaaaccca tcgtcccgat gttttgctga cggacatccg
    34381 catgcctaaa caaacgggca tcgatgctgc caaagaaatt ttggctaaat ttcccaaagc
    34441 catccttttg cttttgacga cattcaaaga tgaagaatac atccgcgaag cattttccat
    34501 cggtgtcaaa gggtatttaa tcaaacagaa cttgcaagcg attattcctt ctgtcaaatc
    34561 cgcttataat ggacaggccg tatttggtaa tgaaatcatc gagactttca ctcaaatgat
    34621 gaagaataaa ccgacgattg atagtcatgc ttatgcttct tttagtgaac gtgagcttgc
    34681 aatcatcaaa gaagtagctg ccgggaaaaa caataaagaa attgcagatg ccttgtactt
    34741 gagtgacggc actgttcgca attacatcag ccaattacta gaaaagctag atttacggga
    34801 tcgcacgcaa ttggctatct atttttatca gcatttgcaa taagtcttat ctaacacaaa
    34861 aaaacctgaa tgatgctcct tcgcatcatt caggtttttt tcacatcttc tattcaattg
    34921 tcccgcaaac catttcctca cgattgaaag aatcaaggat tcgcagggct gccaggattc
    34981 gttggattgt tcggcgtgtt aggcggaaaa ataggcgcct tgtctaacgt tttttctagt
    35041 tttgattctt cacgatcgtc tcgttctaca tcaggacttt cattcaattt gaattctttt
    35101 tccatctcat ctaacttttt aaagtcattg ccagattctt ttgctaattt cgctaattta
    35161 tcttcggttt ctttagccat aaagtaacgc cccccttttg atttcagctt agcatatcac
    35221 actgaaaaaa caaaagaaaa cgctaaccaa cgatcatttt tagctatttc tcatctttaa
    35281 aatcaagcat tttttgccgg tataaaatcg tagggagaac tgttttccat accaatcatc
    35341 ggatctacta actggttatc gatcacttct gctgtaagaa cgaaatcttt agaaacagca
    35401 ggttctacct cttctttctt ttcttctcca acaaatgaag aaaacagatc tggttccact
    35461 gtttccatta cttggttttt cgtttcattc gtcccactgt catcttctac aggtagcttt
    35521 tcttttacaa cccgtggctc ttttgtatag ttcccatctt caaatggatc atttccgacc
    35581 aaagaatctt cataagctgc tagtttggtc cggagagtga tattcatttt atcattatcc
    35641 atgatccttg atttcaacgt agtcatccgg cttgctgatt cattcgctac acaagtgaca
    35701 taggcttgct cttcccctaa caaaatcaac agttccttac tgcgagtcac tgccgtgtag
    35761 agcaaattac gctgaagcat ccgctgatat tgatggacca tgggcaaaat aaccatcttg
    35821 aattcactgc cttgtgattt atggattgag caacaataag aaagagtgat tttattccat
    35881 tcgtttcttt tataagtgac ctcatttgca tcaaactgga taactaattc atccacttta
    35941 tcttcagaat cttttgccaa gatgataccc gtgatttctc ccatatctcc attgaataca
    36001 ttcaattcgg gagtattgac taattggagg actttgtctc ctatccgata aacggtgtcg
    36061 ttccaatgta cttccttttt tccaggtttc cctggattga atatctcctg catcatttta
    36121 tttaacgcat cgatccctgc aggtccccga tacattggtg ctaatacttg gatatcttgt
    36181 gcacggaatc ccttattttt cgctttttcc acaattttag agatcaaggg ttcaatttga
    36241 taagcatcac ttcggaaata ggaacgatct ttttgattct ttgtgaaatc ttttggcaga
    36301 cgtccttctt tgatctcatg ggcaagagga ataatactag aaccatcacc ttgccgataa
    36361 atctgattga gttccatttt gggaatttgt tcgatttcca aaagatcatg gagtacttga
    36421 ccaggaccga cagagggaag ctgatctttg tctcctacta agataacttg catattcgtc
    36481 ggaatcgctt tgaataatgt gttagccagc catgtatcga ccatagacat ctcatcaaca
    36541 atcaaaagtc cgccttctaa ttctttcgta ctgactgaag gtgttttttc ccgtccattc
    36601 agccccagaa gacgatggat cgtactggca ggcagacctg tcgtttcgtt cattcgttta
    36661 gctgctcggc cagttggtgc tgcaagtaga ataggaaaaa tttcttgggt atattttgaa
    36721 ggatccaatt ccagtccgtt caactctgca aaaagattga cgattccgtt gatcacagta
    36781 gttttccccg tacctggtcc tcctgttaaa ataaatagag gggaacggat tgcttcagcg
    36841 atagcttgtt cttgcgaagg tccatactgt atgccgaata ttttttcgat acgccgaata
    36901 ttcttctgca cttctttttc aggataatgg atttcttttt tacgactgag aaggcgctgg
    36961 atcgaattac cgatccccca ttcagaaaag tacaaactgt tttcgtaaag attagtctct
    37021 tcttgttgta tctttccttc ttcaacaagt tggataatta cagtagcaac ctgatcaggc
    37081 tggatttcaa ttgggcgact tgtttctaaa atacggatcg tttgctgaag caatgtctcc
    37141 gccggtacgt atgtatctcc tgtttgaact gcatgctgaa aaatctgatg caagatagct
    37201 gctcgaattc ttctcggtga atctgctgca ataccgattt gttcggctag attatctgca
    37261 cgtttgaacc cgattccttc tatgtcttcg actagctggt acggattttc ttcgatgata
    37321 tccaaagact cgttcttata ggcctggtag atactgaaag ctaactgact accgaacccg
    37381 taacgattca gtcccatgat cacttgatcc atcccgtggt ttagccgaat cgtctcagca
    37441 agcatttctc tttttggctt cgtcaaaccg gagatttgtt ttaataccga aggatcagca
    37501 atgatttttt cgatagcatc ttctcccagc aattctacaa ttttttctgc tgttttcttc
    37561 ccgattcctg ggaatttttc gctggaaaga tacgctacga tcccatttgc agttgttggc
    37621 ttttcttgct catatctttc gagtttaagc tgccttccgt atttaggatg atccactaat
    37681 tctccataaa aacgataagt ctcattttct tgaatatcgc cgaaacttcc ggtgactaca
    37741 atctcttttt cacggtaatc agctgtggtt tcactgatgt gtaccaaaag cactttgtaa
    37801 aaattactag gattttgaaa aaaaatcgcg gcaactgtac caacgaaata aggcatttct
    37861 tctggctgca tcggaagccg cctccttctt ctatagtaaa ggttctggtg ctgtttcagt
    37921 tcctaaaaaa tctgtttggt tccatacttt cagttcatat ggcaataatc tgcaaggctc
    37981 ttcagtctct aaaatcgtca aactactatt gaacaatcca cccatttcac gcaactcact
    38041 tagctctttt cctgtcatcc attgaatggc tgcagttaaa gaagctccat gtcctacgaa
    38101 taaaagtggg ccatctttat gttgggcaac cgcatctgct acaacagttt cgatacgtgt
    38161 gattgccgat tcaatcgttt ctccgtgaaa aggagaagga tcatacagat ctaaatgatt
    38221 acgcaaattc ggcagattat ccggatattt ctggtacatt tcatcgatcg actgaccttc
    38281 taaagtaccc agccctagtt ctcttagctt atctgtataa aaaatctcta ccggacgagc
    38341 aagctctttg ttgatacctt ctgctgtatc acgtgctcgc tttgctgtac tggaataaat
    38401 tgctttaaaa ggaacatatt ttaccttttt cccaaatgct ttgatttctt cgtaactttg
    38461 tggcaaaagc ggagaatcgc cattgctccc ttgaaaacgt ctttcctgat tccattgtgt
    38521 cttcccatgt cgggtaaaat atagttccat acgcagcaaa ctcctgtctt tcttttattt
    38581 tactctataa taaactgtct aatccagctt gtcattttca tagtgatgcg tcaattctaa
    38641 attagggaat ccttgctggc gaagtgcttc atacactatc attgcagctg tattcgataa
    38701 attcaatgag cggacatgct catcattcat agggatgcgt aaacactttt cttcgttttc
    38761 acgcataaat gtttctggca aaccggttgt ttcttttcca aataaaaaga agtgctcttc
    38821 tccatcagca aaattttcgt cactatatac tttatgggca aattttgaga tcaaatgtag
    38881 tttacgatta cctaaaaatg ttaaaaaagc ttctaaatta ctgtggtaag taatatttac
    38941 atcattccaa taatccaacc ctgcccgttt tagatgtttg tcatcagttg aaaaacctag
    39001 tggctcgatt aaatgaagcg gagtattcgt ggctgcacac gtacgggcaa tatttccagt
    39061 gtttgcaggg attaatggtt caaataaaac gatatgattc atcaggatca ttcctctcat
    39121 atttatgttc gtttctattt tctgacaatt tgatttttgc ataaaaaaaa acgtatcaac
    39181 tcggtgtcag tcactgcgag tcgttacgtt agtgaagctc ttctcactaa cttttatcag
    39241 aacaggaacc tgataaaaac caatgaaaaa acaacttgtt gctaatataa caccctcaac
    39301 ttttttttgc aacctctact cattaaaaac tgctagaaaa acgttttctc ttagttcctt
    39361 ttaataattc tatgtcaatt gtaatcttct tcaaaggatt cttatataag tctgctttga
    39421 ttcgctcttc tacctgccat tctaaaggtg acatggccaa aagatctttt tgattttcct
    39481 ctggtatctc aaattcatag gttacacgct gacgttcagt taatttcatt gcttcagcaa
    39541 acttggctac tacacgttca ttggaataac tctgtttttc aggttgattc ggataaaaag
    39601 cctgacgtag ttcctttaaa taattttctt gtggcaccac cttcaacact tctccatctt
    39661 ctgagagtac acgttggaat tcttggtaat gagaaggcga aaagatattt aaaaccgcgt
    39721 tgaatgtttg atcagcaaac ggcaaattgg tcaaatcagc tacacaccaa aatgcttcgg
    39781 cagattgttc tgtagctgcg tagactcctt cttttgaaat atcaaatcca acagcatttt
    39841 gaatcttttt caattttgaa agttcattca agaagctacc ttctccgcag ccaacatcta
    39901 atagcgagac ttcttcaggt aaatgctgcg caatagtctc aataacgggc tgatacatgc
    39961 cagattggat cattctacgc cttggaacga acattccttg atcgtattct gttttaaccg
    40021 catgggataa aaaataaagt gttccttttt tggaaaggtc aaaacggtga ttatttttgc
    40081 atatcaagcc attatcagac ttttttactt cttcttggca aaccggacag cggaacagct
    40141 gatgattttc tgccaaaaac tggcgtcctc ggtcaatttt ttttaacata gttccccctc
    40201 atttctgttt tatcttaccg ttagatgctc actttggcaa ctgctaaaaa tatgatacaa
    40261 tgcctagaag aaagtgaaag ataaaggaga agattagatg atcaaagaat tttgcgctga
    40321 aaactacaca tctattcctc tagcaattgc tagaggagcc gatcggatcg aattgtgtga
    40381 caacttagcg gtcggtggca caaccccgag tactggtgtg atcgaagaaa cgttaagtta
    40441 tgctggggaa aaagatatcc ccgtaatgac gattattcgt cctagagggg gcgacttcgt
    40501 ctataacgat atcgaattga aaatcatgca tactgatttg attgaagcaa agaaattagg
    40561 gacggatggc gtagtatttg gttgtcttac tccttcaggc tggctagacg aagaggcctt
    40621 agaactattg atcgaaacag cagaagggtt gcagatcacc ttccatatgg cttttgacag
    40681 tattcctgaa gatcggcaat tcgaagcaat cgattggctg gtagaacatg gtgttgaccg
    40741 tatcttgact catggcggtc ctgctgacaa aactattgta gaaaatttcc cacggcttaa
    40801 agaattgatt gcctatacag atggccgctt aatcattttg cctggtggtg gtgtcactgc
    40861 agagaatgca gcacatgtag ctaaagaatt gaacgtaagc gaagtacacg gaaccaaaat
    40921 cgtggatctt cagttataat cttataaaca aaaaaacatt tgcaacaggc ataatgctta
    40981 ttgcaaatgt ttttgttttt ttacataaaa tagcccataa ataaagaagc aatattgaaa
    41041 taaatcagta cactcgtcaa atcgctcaaa gtcgtgataa agggaccgct tgctactgct
    41101 ggatcaaatc ctagacgatc cattagcatc gggattaaac tacctgcaag attcgccact
    41161 gtgattgcaa atagcatagc catccctatg acaaacccca agataaaatt atgtttccaa
    41221 aaacctacga ttagaaaaat cgtgatcccc gtaacagctc ctgtgacaac gccggtcaag
    41281 acttcagcca aaacaagtcg aacaaatcca tgatctttct catcagaaat cgcaagtcga
    41341 cgaacagcca cagctagcga ctgggttcca gcattaccgg cagttcctgt aatcagcgaa
    41401 atgaagactg ctaaaatact cgcttcactt accaagtctt catagtgact gattaaagaa
    41461 gcggtcgaca tacccaaaaa caacagagtg atcagccaag gcaaacgtcg agacgctgcc
    41521 tttactggat tttcattaat ttcttccaca ttgaccccgg caagtcctga ataatcgcta
    41581 gcagcttcat catcaataac gtcgatgata tcatcgactg tgacgattcc taacaaatga
    41641 tcatcataat cggtaacagg aagagctaag aagtcataat ctctaaatgt ttgtgccaca
    41701 tcttcttggt cgtcgcctac atgaactgaa agcactcgtt cgctcatcag atcagaaatc
    41761 attgcgtcgt catcattcac gattaggtcg cgcagagaaa taacccctac tagctgacct
    41821 tcttgattaa cgacatagat gtaatagatt gtttcagcta catctgcttc gttcttcagc
    41881 acgtacatcg cagagcgaac agtttgattg gctacgatcg aaacaaattc agttgtcatg
    41941 atcgcgccag ccgtttcatc ctcgtaatgg agaagttcct tgatttcact ggcatcgtct
    42001 gccgacatca aacttaaata tttagcaatt tgctttttat ccagcgtatt aagcaagtca
    42061 acagcattat ctgtatacat ctcagaaagc atatcagctg catagctagg acgcatttca
    42121 tctaggtatt ctttcatatg ttcgttgtct tcttcaatca catcgaacat atccgctaat
    42181 tctttaggcg acagatagga atagatcagc tgcctgttct cttctgtaag agattggtag
    42241 aattgcccct gctcatagat atgcatctct aaaaaactgt ttctgaatgc ctgaatatta
    42301 ttttccctta aatcttgagt caactgggca aatctttcct ctaattcttg attttcatcc
    42361 aaaccaatct cacctcgcct tattcttcaa accgtctcac aactgcctcc atgtctgcgg
    42421 ctaaaggctg cttgaactcc agcaactctt ctgtaaatgg atggtaaaaa cgcagatcat
    42481 aacaatgaag ggcttgtctt tttattccca gatccattcg cccgccatac atatcatcac
    42541 cgagcagggc acagccaatt gcggaaaaat gaactcttat ttgatgtgtc cttcctgtat
    42601 gcagctggat atcaactaac gcgatttctg ttagtcgctt ctctatccag tactcagtct
    42661 gtgccgaacg tccttcatca gagaccattc gcttcaatag cgaagaataa tctcgggcga
    42721 tcggcaaatc gatatgtcca tgtgccgata aatctgaaag ccttccactg actaaggcct
    42781 gatatttttt cacaaacttc ttttcacgaa gttgcatatc taattttgcg tgcgcaaatc
    42841 catgctttgc aaaaagcatc aaaccagatg tatcccgatc tagtcgcgtc accacatgga
    42901 ttacttggtt ttcgtaacct tgttttttat aataagcctt tacacgatta gccattgtcc
    42961 catttggatg gtattgggca ggaatagacg aaacgcctgc tggtttattt actaccagaa
    43021 cgtgttcatc ttcatacacg atatcgatcg gtgtttcatc caaaagtacg gtttcatgtt
    43081 ctccttcagc aggaatcaca atggttacct tatcatcctt agctagagaa aacaagacat
    43141 tttctacttg atcattaact ttgatttgac cgccttgaaa ttttattttc gctaacagac
    43201 cctttgagat ccctttttct tttaaaaaat attttacttg ctgagaatga tctttctcat
    43261 aatcccatgt aaactgcata tcaatcctca ctaataaagg catctttcac acgatgccaa
    43321 aaatgcatat gtcgataaga agcaaaatgg atccgttctt ctgctattcg ataataaatt
    43381 gagcggatat ttgcttttgc gacattcaat tgatcaaccg ttacaagata atcttctgta
    43441 ttttccaatt ttatttctag ccattcatca tgggcaatca cgatcggaga acccaaagta
    43501 cgaaacactc gattattcag tgaagcaatc tctgccaact gaaaggcatt gatactagga
    43561 tggatcacag caccgccgac acttttatta tatgctgtcg aaccagtagg cgtagaaatc
    43621 gtcaatccat ctcctctaaa actttcaaac agctcgtttt tgatgtatac atcggctacc
    43681 atcgtacgat tggcccgttt aatcgttgat tcattcagtg ctaaaaaatg ctggtccggt
    43741 gtttcatcca aataactgat ccgaacatcc agcaagggat aactaacact ttgttcccgg
    43801 ttcgttcgta agctgtctac tagttcttcc agctcgtaat cccgccaatc cgtgtaaaac
    43861 cctaaatgac ctgtatgcac gcccaagaaa cgcacatcat tcagacaatg actgtagtga
    43921 tggaacgccg acaataatgt gccgtctccg ccgactgaga tgaccaattc tggctcatga
    43981 tcatctcgtt gaatccctgc ttgttctaaa agtgcgttca aacgttctgt cacttctata
    44041 gtctttgctt ctttattatg aacgatcgct actttcatcc cctgcctcct ctctttcgct
    44101 ttctttcttc aaaagcgttc gatagtagac atcatctgat tttcctcttc catgggagaa
    44161 taaccgctgt gcttcttgga tttcttcacg gatactggac atctcttcat ccaactgata
    44221 ggcagcttct gccgcacgct gaagtcgttc actcatttct tctggaaaaa cgccttgata
    44281 tttataattc agcgaatgct caatcgttgc ccagaaattc atcgcaagag tccgaatctg
    44341 gatttccgcc aaaattttct tctcaccgct gatcagctgt acaggatact cgatcaccag
    44401 atgataagaa cggtagccac ttgctttttt atttgtgatg taatctcgtt cttggagaac
    44461 tttcatgtct tttcttttac ggatgatctc aacaacttga tggatatctt ctacaaactg
    44521 acacatgatc cttaacccgg caatatcaga catttcttct tctaaacgtc ccattggaat
    44581 atgccgtaat tgtgattttg ttacaatact atcgactggc ttgactctcc cagtgacaaa
    44641 ttcaatcggg acatgtcgat tttgttcacg gaactgtttg cggatccccc gcagtttcac
    44701 tttcaattct gatacagttt gttcatatgg tgctaaaaat gcttcccaat ctcgttccat
    44761 agctgatacc tctacttcat taacattcat tgtttattct accatatatg caaagttttt
    44821 tcgtactctt gatttatgca ttgaccaaaa aaagaaaatt aggcaaaata gacaaaaagg
    44881 agtcgatcaa atgacacaaa tgcttgaaat cgaatataaa tctatgctga caaagcaaga
    44941 gtacgagata ttcatcgctc actatcaatt aacagataaa gatttccgtg tacagactaa
    45001 tatttatttc gataccgttg atgaacagct gaagaaacaa aattgtggcc tacggatccg
    45061 tttcgttggt catcaagctg aatatacatt aaaaacacct gctgaaaaag ggcgtctcga
    45121 aacaacggat acctttataa cagaagaagc agaagcattt atcttcgaaa aacgtctgcc
    45181 tcgttcaggc gcagtttttc aaaaactttc tgatttgaat atcgatcctg cttcattaat
    45241 ccaaaccgga aagcttacaa caaaacgggc tgaattccct attgaagaag gacttttagc
    45301 tattgatgaa agctggggag acaatctaca cgattttgaa ctggaactag aggtagggga
    45361 tgcatctgtg ggaaaaattg cttttataaa ttttttaaaa cgtttttctt taccttatcg
    45421 tcctgctaag aacaagatcc aacgtatgct tgaagctaaa aattagaaac ctttcatttt
    45481 cacaaggcac ttgagttcat ttttttactt ttttctgtta agttatagga tgtgaaagaa
    45541 tctagtcact agttgtatag aatctatttt gggggagatt catatgatcg aaatctattt
    45601 atttgttaat cctttagggg aatactgttt cgaaatggaa caacagttat tacaatttat
    45661 cgaagaagag tacgggacac catcaaaaga aaagatgcaa tttcgctttc ttcctttggt
    45721 caatttgcag acaattgggg atgtcatgca acaaaaagga atttctcaga acaatcttga
    45781 ggaacgtaat cacttattct caatgactta ttctgcagca ttggactgca aagcggcgca
    45841 gtttcaagga aagaaaaaag gccgccgttt tttgatcaaa ttgcaagaag cagtcggctg
    45901 taatgacata ccatattctc aagaacttgc tgaatccata tttgaagaaa tcggcggcga
    45961 tttgaatatg tttaaagaag atcgtcagtc tgacttcgtt aaagaagtat tcttttctga
    46021 tcaaaaaatt gctcgggaaa tgggcatcac aaaacaccct tctgcagtcg tttacaatta
    46081 tgcctgcgac cgtgattttg gtgttttagt agaagtaaaa ggtacaagta gcctttggag
    46141 aatcacagag ctgtgtaaaa caacagataa ctcatatcaa gtttttaatg gtgaacattt
    46201 acatacaaat gattctcagt tccattcaag aactagccat ttacacttgc tttctaatta
    46261 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
    46321 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
    46381 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
    46441 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
    46501 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
    46561 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
    46621 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
    46681 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
    46741 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
    46801 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
    46861 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
    46921 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
    46981 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
    47041 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
    47101 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
    47161 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
    47221 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
    47281 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
    47341 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
    47401 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
    47461 taaattgttc gtaaacactg tacaatccct taaatgctgc ttcgcgcacc cttcgatccg
    47521 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
    47581 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
    47641 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
    47701 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
    47761 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
    47821 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
    47881 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
    47941 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
    48001 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
    48061 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
    48121 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
    48181 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
    48241 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
    48301 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
    48361 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
    48421 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
    48481 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
    48541 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
    48601 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
    48661 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
    48721 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
    48781 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
    48841 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
    48901 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
    48961 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
    49021 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
    49081 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
    49141 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
    49201 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
    49261 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
    49321 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
    49381 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
    49441 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
    49501 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
    49561 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
    49621 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
    49681 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
    49741 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
    49801 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
    49861 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
    49921 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
    49981 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
    50041 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
    50101 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
    50161 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
    50221 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
    50281 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
    50341 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
    50401 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
    50461 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt t
//
LOCUS       DAZUKZ010000009        50215 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000009 DAZUKZ010000000
VERSION     DAZUKZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.009"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="ACG8EF_000497"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="ACG8EF_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFS4945849.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ACG8EF_000498"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ACG8EF_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFS4945850.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="ACG8EF_000499"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="ACG8EF_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFS4945851.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="ACG8EF_000500"
     CDS             1642..2274
                     /locus_tag="ACG8EF_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS4945852.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="ACG8EF_000501"
     CDS             complement(2276..3181)
                     /locus_tag="ACG8EF_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4945853.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="ACG8EF_000502"
     CDS             complement(3266..3925)
                     /locus_tag="ACG8EF_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4945854.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="ACG8EF_000503"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="ACG8EF_000503"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFS4945855.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="ACG8EF_000504"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="ACG8EF_000504"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFS4945856.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="ACG8EF_000505"
     CDS             6168..7253
                     /locus_tag="ACG8EF_000505"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFS4945857.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /gene="fni"
                     /locus_tag="ACG8EF_000506"
     CDS             7250..8314
                     /gene="fni"
                     /locus_tag="ACG8EF_000506"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFS4945858.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="ACG8EF_000507"
     CDS             complement(8563..9366)
                     /locus_tag="ACG8EF_000507"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFS4945859.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ACG8EF_000508"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ACG8EF_000508"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFS4945860.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="ACG8EF_000509"
     CDS             complement(10244..11251)
                     /locus_tag="ACG8EF_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS4945861.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="ACG8EF_000510"
     CDS             complement(11258..11902)
                     /locus_tag="ACG8EF_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS4945862.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="ACG8EF_000511"
     CDS             complement(12002..12760)
                     /locus_tag="ACG8EF_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFS4945863.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ACG8EF_000512"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ACG8EF_000512"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFS4945864.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ACG8EF_000513"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ACG8EF_000513"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFS4945865.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ACG8EF_000514"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ACG8EF_000514"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFS4945866.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ACG8EF_000515"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ACG8EF_000515"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFS4945867.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ACG8EF_000516"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ACG8EF_000516"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFS4945868.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="ACG8EF_000517"
     CDS             18158..19027
                     /locus_tag="ACG8EF_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4945869.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="ACG8EF_000518"
     CDS             complement(19153..20052)
                     /locus_tag="ACG8EF_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4945870.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="ACG8EF_000519"
     CDS             complement(20217..21041)
                     /locus_tag="ACG8EF_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4945871.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="ACG8EF_000520"
     CDS             complement(21052..22347)
                     /locus_tag="ACG8EF_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFS4945872.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="ACG8EF_000521"
     CDS             complement(22363..22842)
                     /locus_tag="ACG8EF_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFS4945873.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="ACG8EF_000522"
     CDS             complement(22848..23876)
                     /locus_tag="ACG8EF_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFS4945874.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="ACG8EF_000523"
     CDS             complement(23893..24999)
                     /locus_tag="ACG8EF_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4945875.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ACG8EF_000524"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ACG8EF_000524"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFS4945876.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="ACG8EF_000525"
     CDS             complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="ACG8EF_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFS4945877.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="ACG8EF_000526"
     CDS             complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="ACG8EF_000526"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFS4945878.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29970..30668
                     /locus_tag="ACG8EF_000527"
     CDS             29970..30668
                     /locus_tag="ACG8EF_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS4945879.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30836..33475)
                     /locus_tag="ACG8EF_000528"
     CDS             complement(30836..33475)
                     /locus_tag="ACG8EF_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4945880.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34094..35125)
                     /gene="queA"
                     /locus_tag="ACG8EF_000529"
     CDS             complement(34094..35125)
                     /gene="queA"
                     /locus_tag="ACG8EF_000529"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFS4945881.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35318..36493
                     /locus_tag="ACG8EF_000530"
     CDS             35318..36493
                     /locus_tag="ACG8EF_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4945882.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36497..37138
                     /locus_tag="ACG8EF_000531"
     CDS             36497..37138
                     /locus_tag="ACG8EF_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4945883.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37135..38052
                     /locus_tag="ACG8EF_000532"
     CDS             37135..38052
                     /locus_tag="ACG8EF_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4945884.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38049..38720
                     /locus_tag="ACG8EF_000533"
     CDS             38049..38720
                     /locus_tag="ACG8EF_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4945885.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39261..39749)
                     /locus_tag="ACG8EF_000534"
     CDS             complement(39261..39749)
                     /locus_tag="ACG8EF_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFS4945886.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39950..40216)
                     /locus_tag="ACG8EF_000535"
     CDS             complement(39950..40216)
                     /locus_tag="ACG8EF_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS4945887.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40216..40479)
                     /locus_tag="ACG8EF_000536"
     CDS             complement(40216..40479)
                     /locus_tag="ACG8EF_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4945888.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            41000..41140
                     /locus_tag="ACG8EF_000537"
                     /pseudo
     CDS             41000..41140
                     /locus_tag="ACG8EF_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(41282..43105)
                     /locus_tag="ACG8EF_000538"
     CDS             complement(41282..43105)
                     /locus_tag="ACG8EF_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4945889.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43703..46408)
                     /locus_tag="ACG8EF_000539"
     CDS             complement(43703..46408)
                     /locus_tag="ACG8EF_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFS4945890.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(46714..47661)
                     /locus_tag="ACG8EF_000540"
     CDS             complement(46714..47661)
                     /locus_tag="ACG8EF_000540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFS4945891.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            47851..48120
                     /gene="rpsN"
                     /locus_tag="ACG8EF_000541"
     CDS             47851..48120
                     /gene="rpsN"
                     /locus_tag="ACG8EF_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS4945892.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48296..48658)
                     /locus_tag="ACG8EF_000542"
     CDS             complement(48296..48658)
                     /locus_tag="ACG8EF_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4945893.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48669..49790)
                     /gene="alr"
                     /locus_tag="ACG8EF_000543"
     CDS             complement(48669..49790)
                     /gene="alr"
                     /locus_tag="ACG8EF_000543"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFS4945894.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(49806..50156)
                     /gene="acpS"
                     /locus_tag="ACG8EF_000544"
     CDS             complement(49806..50156)
                     /gene="acpS"
                     /locus_tag="ACG8EF_000544"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFS4945895.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
BASE COUNT        14178 a        10889 c         9190 g        15958 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    28021 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    28081 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    28141 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    28201 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    28261 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    28321 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    28381 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    28441 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    28501 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    28561 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    28621 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    28681 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    28741 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    28801 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    28861 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    28921 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    28981 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    29041 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    29101 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    29161 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    29221 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    29281 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    29341 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    29401 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    29461 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    29521 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    29581 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    29641 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    29701 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    29761 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    29821 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    29881 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgatacg tggtatattg
    29941 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    30001 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    30061 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    30121 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    30181 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    30241 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    30301 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    30361 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    30421 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    30481 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    30541 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    30601 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    30661 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    30721 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    30781 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    30841 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    30901 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    30961 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    31021 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    31081 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    31141 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    31201 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    31261 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    31321 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    31381 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    31441 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    31501 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    31561 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    31621 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    31681 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    31741 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    31801 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    31861 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    31921 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    31981 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    32041 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    32101 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    32161 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    32221 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    32281 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    32341 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    32401 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    32461 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    32521 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    32581 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    32641 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    32701 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    32761 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    32821 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    32881 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    32941 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    33001 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    33061 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    33121 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    33181 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    33241 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    33301 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    33361 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    33421 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    33481 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    33541 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    33601 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    33661 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    33721 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    33781 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    33841 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    33901 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    33961 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    34021 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg
    34081 gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct
    34141 cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta
    34201 acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga
    34261 attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat
    34321 taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt
    34381 tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga
    34441 attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta
    34501 accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta
    34561 attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat
    34621 agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca
    34681 tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc
    34741 gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga
    34801 ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt
    34861 gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata
    34921 aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt
    34981 caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta
    35041 aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa
    35101 aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt
    35161 ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt
    35221 cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag
    35281 atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc
    35341 aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg
    35401 tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat
    35461 caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga
    35521 aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat
    35581 gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca
    35641 agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat
    35701 gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg
    35761 tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc
    35821 aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac
    35881 gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat
    35941 catgagtgaa ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc
    36001 aaacgatttc gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc
    36061 gacaagagtc ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt
    36121 acaagaagca atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa
    36181 ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt
    36241 atccagtgtc ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct
    36301 tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga
    36361 acagaacaaa gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt
    36421 gatttgggga gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc
    36481 taaggaggtc tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa
    36541 aggttttgaa catctttata tttcaggact tgctttgatc attggtatca tcattgctgt
    36601 accgcttggt gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac
    36661 cagtgccttg cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg
    36721 cgtaggtaaa ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg
    36781 taatacgtac attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat
    36841 ggggatgact gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat
    36901 catggctggt attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta
    36961 tatcggcgct ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga
    37021 tctcattttg gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct
    37081 aggtctcttg gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa
    37141 cgtatactaa aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc
    37201 cctggtcttg ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca
    37261 gaagcacaga tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat
    37321 accgcaatca tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga
    37381 gatgcttcaa tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt
    37441 cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga
    37501 tacggtcaaa cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga
    37561 aaagacaccg cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat
    37621 gaattagtag ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc
    37681 ttccaagaaa cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt
    37741 tatgacgcag tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg
    37801 atcgccagtt atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat
    37861 gctgctccag ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta
    37921 aataagttag gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat
    37981 aataatctag tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt
    38041 gaagggaagt gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc
    38101 aggaaaatgg ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg
    38161 gtgtcctctt tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta
    38221 aattagcaaa ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca
    38281 tgatctccat tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat
    38341 ttttgtattc cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca
    38401 aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg
    38461 tagagttacc actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg
    38521 ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta
    38581 tccgaggaac caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg
    38641 cattaatggc gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag
    38701 ccagccgtac atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg
    38761 gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc
    38821 tccttatggg taaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc
    38881 atactgccaa cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg
    38941 cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa
    39001 tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc
    39061 atttaagcct tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact
    39121 ggtgactgcc gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc
    39181 atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc
    39241 ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga
    39301 tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa
    39361 aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc
    39421 atggccgaaa gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat
    39481 gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta
    39541 tcttttccaa aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga
    39601 ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt
    39661 gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat
    39721 gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt
    39781 caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat
    39841 taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt
    39901 tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat
    39961 gtgtccaggc agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc
    40021 gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa
    40081 aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt
    40141 tctttaaatt cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga
    40201 ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact
    40261 gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt
    40321 ttcttgaatc gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc
    40381 actaataatc tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt
    40441 tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga
    40501 tataattata gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca
    40561 gcaaactttt cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc
    40621 cacgctcact tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca
    40681 acgtatcatc tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat
    40741 acacgaaggt tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg
    40801 cacgaaagat ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct
    40861 caaaaatgat tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc
    40921 ttagaccgtt gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa
    40981 atggtcactt gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga
    41041 ggtaaaggaa gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa
    41101 ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt
    41161 tcatacgctg ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca
    41221 aaacagaccc tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg
    41281 tttatttctt caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc
    41341 gcaatcttaa ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta
    41401 caaattgagg aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga
    41461 tatctacata acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa
    41521 aacggacatc tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat
    41581 cttcgtctag cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca
    41641 acgcaccgat atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat
    41701 aatcatgtag ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat
    41761 catggatctt ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt
    41821 cccctaaccg atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa
    41881 tattggcaat cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc
    41941 ctgtttgaga taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt
    42001 ctgttgagcc atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc
    42061 cattggaata gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt
    42121 ttgccagatt gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta
    42181 aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt
    42241 taccaatttg agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga
    42301 taaatgtgat ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag
    42361 cggcattttt tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc
    42421 cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg
    42481 gcacagccgc tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat
    42541 ataaaccaac aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata
    42601 ggaacgatgc agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg
    42661 tcacagaaat agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag
    42721 cacctgctga gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg
    42781 aaatcaagcc agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat
    42841 gaggataagc atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa
    42901 caaaagcggc aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga
    42961 caacgatttg ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca
    43021 aagcagcaaa tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta
    43081 ctaacaacct ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag
    43141 ttttatagtc gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag
    43201 tcaataaact ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa
    43261 cgctttaata cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat
    43321 aaaaggatag aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg
    43381 gaagagagat gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt
    43441 tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc
    43501 aaattaaaaa cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag
    43561 cttttttaca acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa
    43621 aaaacggcaa aatatgagga actatttttc catttttttc tctaatactc aggtcaaaac
    43681 actgatttca caacctcttt ttttaatgaa aaatatggct ctcatgcgcc gcttctttga
    43741 atttcgtcat tttttcttcg atgaacggat agattgccgt tcctacgatt ccaaagacaa
    43801 caaacaaacc gatcatgatc cagattgctt tcgtagcatt tggccaataa attcctcctg
    43861 cagattcccg taacaagttt accgcatgag taaatggcaa gaacggattg atcatttgga
    43921 agaatttccc tgagacttgg atcggatagt tcccgcctcc gccagagatc gacagtacta
    43981 agatgataat agcgatccct ttaccgacat tatcgaataa tgcagccaag acatagacga
    44041 tcatcatgaa cgctaaggcg atcaagacag caaatgacac gctgtagacc ggattttgga
    44101 catctactcc tagtaagaaa atattcccta atgtaacgat caagctttgc gcaatcgcca
    44161 ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt
    44221 ctttctttcc taaatagtat tctgtcgtag caacacttga aagaagaaca gcgccgaccc
    44281 acagacatag tgcagtataa aacggggtgc tagctgaacc gttatttgcg attggataaa
    44341 gcgcattggt ttgtagctca acaggttgtg taaagaagtc actttctttt gttgcgtcta
    44401 gtttcaataa tttgatgact tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt
    44461 ctgctgcttt ttggatacca gctttgatat ttggccagtc attgttgatc aagtccgcag
    44521 cagcgttcac agctgtttcg acttctggca ttttttcatt tactgttgtc aatgtaccgg
    44581 tgatttcatt ttttagtgct ggccaatcat tttggataaa atcagcggcc aatcctaatt
    44641 tttgctgaat gatcggtaat tcattgttgt ataggtctgc cccttcattg atgccattga
    44701 cgatggtatc catatttcca ttcagcattg tattggcatc atggatttct tgtccaattg
    44761 caggcatttc tttttgatat ttttctaaaa ttgccactgc attcgttaca gttgctttcg
    44821 ttgagctgag aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag
    44881 tcccaatcgt atcgatgact tggtctagta gagaagtgac agtggaactg atttgacctg
    44941 tattgatacc agcaatgatg ccattgatat tattaacaac ggcatcaatt tgatctagca
    45001 attcatcaat agccttcgtg tcttcatttt gaacagctgt gttcaaagca gataggcggt
    45061 tttgcagatt gcttagcaag ggttttaatt gatttagctg atcaataatt ggctgtaatt
    45121 tcgtatttcc agcacttgtt tgcaatttag tcaggaactc gaccagttta tcgattgcat
    45181 tttgttgttg agatattccg tcaataaagc cgttcaatac attgctgatg tttttctttt
    45241 catcatcagt cagcttacca tcttcaatag ctgttcttac agtatctatt aacgaacctg
    45301 tcgttgttgc cacttgtcca attgacttca atgtaacatc tacactgttt gtgatgcttg
    45361 gtatcgcatc tttcagctga gaggcacctg attttgtttg gtttgctaga tcacttgcct
    45421 gattttctaa atttttcaca tctggcaaga tatcttgtac ttgttggatg atcgtcaagc
    45481 cttgttttgc ttcatcgatt ccttggttca ttgtctcttg gatcgaagca aaatcgccgt
    45541 cgatctctgc tagttgctta ccggcatttt ggatctctgg gattttttct tgcaaatcaa
    45601 ggatgatttt tgcttgatct ttcaactcag gcattttgtc attcaatgca acgacttttt
    45661 cacccatctc atctacttta gggatgtaat ccacgaattc atttgcttta ttcagttttt
    45721 ccttgatttc aggtagttga ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg
    45781 tgtccaaatt ttcatctgtt gataaaatca tatctttaac tttattgata cttactaggt
    45841 tcgtatcgat gtcgtaacct atctcattga atactttcaa taaagtcgaa ctggctgttt
    45901 tgataaattc atttgtgatt tgcgattgga tcgaacttgc tcctttatcg gtgattttcg
    45961 gtgcaatcgc attgattttt tcatttaccg tatattcgat ttttggtttc ttgatatcac
    46021 cactagtaaa actcagcaga tcttctgaga aatcctttgg caagtaaata cctgcgtaat
    46081 actttccgga acggactccg tcttctaatt gttcttttga atcaacgaat tgccatccta
    46141 attgtttatt tttatgaagc gattcgatga cttgctcccc aatggccact tctttacctt
    46201 gaaactctgc tggcttgtct gcactgtaaa cagcaattgg cagctctcca gtatttccat
    46261 atggatccca taatgctttg atgttaaacc acgcatatag cgaagggatg atcatgatcg
    46321 cgatgatcag aagtgttgca atcgggtttt tgaaaatccg tttccagtca agcaaatata
    46381 atcgaagcgt atttttgatt gactgcatag ttctccccct tcttcttttt tcagctgaat
    46441 tactttacca tgtatttcat acttttttca atcggctttt ttcaaaaaag tcgaattttt
    46501 ggacatatca cggcaattgt ctcattgtaa tctcttacat attgagaaat aattaaggat
    46561 tacagccttt actttatcat gaaataaaag ggaaagataa ttttatgcca aagtttttat
    46621 ctattatacg tatacgcaaa aaagctaaaa cattttttgt ctttgcccct acaaaaatgt
    46681 ttcagctttt ttcgtttttt attcctgtat ttcttatttt tcgaatgaca ggatgatttt
    46741 cccagcagga ggatgtcctt caactagctg atgtgcttgg atgacatgct taagtgaagc
    46801 agacagattc attgaaataa aaacatgaaa ggctcctttt tggtaggctt tgattatgcc
    46861 agcaaaggct tctgcatcta gatattcctt tcttggaaca aacgattcat aaaagccttc
    46921 ctttttttgc cgcaaatcta gatctgggag gtcatttaat gcaacatacc tgccgcctgg
    46981 tttcatgatc tggatgccgg tttctccttt gatacttcct ttcgtcgcat cgattacaag
    47041 gtctgcctga tcagcaaatt gcagacctgg atttgtttta tcataagcag caaatgctga
    47101 agctcctaat ttttttacct tttcctcatt tttactggat gcactggtta agattcggat
    47161 tcccttttca tggagaagct ggatcagact tgacccgacc gcacctgatg ctcctagtat
    47221 cattacagta tctgtcggct ggatttccgt caaatggttg attagattat aagccgtaat
    47281 tcctggcgtg accattccgg ctgcttcttc ccaactcatt ttatctggaa tttttgctac
    47341 ttttgctgat ggcaacacga cttcttctcc gtaagtcccg ccgactgcat ggacagctac
    47401 tcgatcacct acatgaacag ttgtgacatc tgaaccaatc tctgtcacaa taccagcgcc
    47461 atcattacct ggtacataag ggaatttcaa tgttcgtatc tctttcattt ctcctaaacg
    47521 caaggcaaca tcataaggat tcacactgaa tgcttttatt tctacacgaa catgtccaga
    47581 accgacttct ctgggatgag catcgatttc ttcaaaaaca tcttgtgctt ctccgtaacc
    47641 gttaattgca aatcttcgca tggtcaccat ctcccttttt ctttaagttt acgtttatgc
    47701 ctttattttg acaaaaaaaa tgtcttttcg ttttggcgca tgattcaaaa caaaatagtt
    47761 gtcttttttc gcgtaacgtg ttaaattatt tatctaaaag cgtaaagatt acgatttacg
    47821 ctaataaaaa ataattggag gaatttgttg atggctaaaa aatcaaagat tgccaaagca
    47881 aaaaaacagc aagaaacgat tgaacgctat gctgctttac gccaggaatt aaaagcgcaa
    47941 aaagattacg aaggcttgcg taaacttcct ttagatgctc gtccagaacg attgaaaaat
    48001 cgtgatttaa tcgatggacg ccctcgcgga tacatgcgta aattcgggat gtcccgtata
    48061 aaattccgtg aattagcaca taaaggtttg attccaggag taaaaaaagc cagctggtaa
    48121 tccaagtctg gctgtaatgt aaaggaggag ggcgcatgga ccactccttg gatcatcaaa
    48181 aaagatacgt tcgcttcttc tagcagaaat gctggtacta gaagcagacg tatctttttt
    48241 tacgcacagc tggtcctttt ttatatgtaa aggctgcatg gttacttcgc tatatttatt
    48301 tagtaacagg agtcagtgct tcgataccca cactaatttt caatgctcgg tcaacttctg
    48361 ccataatggg tcgatccaaa tggcagacct tttctttcaa tcttgattta tcgatcgttc
    48421 tgatctgctc caacaggata acggagtctc tttctattcc tgttccagta gctttgatcc
    48481 caatgtgagt cggaagctta ggtttagcca tttttgccgt tatggccgca acaatgatcg
    48541 tcgggctaaa atggttacct agattatttt gaataaccaa aactgggcgt gttccgcctt
    48601 gctccgatcc cacgactgga gaaagatcag caaaataaat atctcctctt tttaccatag
    48661 gatcctcctt attcttgata ttctcttggt atccgctgtc ctaatccgca agccacttca
    48721 tagtggatcg tacctaattg atctgctata tcctgcatag tgatttcttt gtctgcattt
    48781 ttgccgataa gcgtcacttt tgttccgaca ggaaattctt gaggcaatcg gatcatcagt
    48841 tgatccatac atacacgccc aatcgtttca cagtagttcc cttctaccaa tagagaaaaa
    48901 ccctgcatct ttcgtggcca gccatctgca taaccgatcg gtatcgttcc gatccactct
    48961 gcttcaggag tgatatatgt ttctccatag ccgattcctt cacctgctgg gagttttttg
    49021 acttgaatca attcagaaac cagctcaagt gctggctgca atggataaac ttccggtagt
    49081 gcgtgcccag agggattaag tccgtacatt gctactccat aacgaatcat attgccgatt
    49141 gtttcatcat gccataaagc cgttgcacta ttgctgacat gtacatatcg aggaagttcg
    49201 ggaagttttt tcagcacttc tataaagcgt tctttttgca gattccaata tgtatcatct
    49261 gcctgatctg ctgtagaaaa atgggtaaat aaaccttccc attctaatac acgactttct
    49321 ttgatgaatg cagctgcctc ttttacttct tcaggtgtac aaaatccgat acgacccatc
    49381 ccagtgtctg cttttagatg aatcgaaaga ggcgtctcta gcttactttt ctctaaaaca
    49441 gcagctgctg cttgcaacca ttcccgtgta cctgcagtta ctgataaatc atgagcaaca
    49501 gctacagtca gtgcgtcgta aggaaccata ttcaagataa ggatgggctg agtaaatcca
    49561 gcttctctca actcgactcc ttcatctaaa tttgaaacac aaaagcccga agctccacct
    49621 gcaactgctg cctcagctgt ttcaatagct ccatgaccgt atccgttcgc ttttactact
    49681 gcaaacaatt cttttccttg cggaagtctt gcaacttcat ttgctacatt ttcagttata
    49741 gcttttttat gtatcactgc tttcgttggg cggtgccaag cgacaaccat cgtttttcct
    49801 tctttctagt cttccaaaat aatttgtgca aaagctgttt catttgtatg agtaatactg
    49861 acccatacat tgccttcatg aggtgaacgc gtcacaacag gctgaccatt ttcatttttt
    49921 aaaatctcga catcttgaaa ggtaactttt ccgatcccag ttccccaagc tttagagaaa
    49981 gcctctttgc aagcgtaacg cccgcctaaa aattctactt ggcgatgaaa aggcaaggac
    50041 tggaatagtt tcatttcatc agaggtcaag attcgtgcaa tgaattttgg tttttcttcg
    50101 atcagccgag tgatcctagg aagttcgact gcatcgatcc caattccttt tatcatatgc
    50161 ttttctcttt tcaataaatt tagaacactc tcattctacc aaattataac cattt
//
LOCUS       DAZUKZ010000010        45512 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000010 DAZUKZ010000000
VERSION     DAZUKZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.010"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8EF_000545"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8EF_000545"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFS4945896.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8EF_000546"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8EF_000546"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFS4945897.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8EF_000547"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8EF_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFS4945898.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8EF_000548"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8EF_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFS4945899.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8EF_000549"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8EF_000549"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFS4945900.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8EF_000550"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8EF_000550"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFS4945901.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACG8EF_000551"
     CDS             complement(8053..8523)
                     /locus_tag="ACG8EF_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFS4945902.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACG8EF_000552"
     CDS             complement(8569..8994)
                     /locus_tag="ACG8EF_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFS4945903.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACG8EF_000553"
     CDS             complement(9061..9330)
                     /locus_tag="ACG8EF_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFS4945904.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACG8EF_000554"
     CDS             complement(9341..10936)
                     /locus_tag="ACG8EF_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4945905.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8EF_000555"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8EF_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFS4945906.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8EF_000556"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8EF_000556"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFS4945907.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACG8EF_000557"
     CDS             15402..16376
                     /locus_tag="ACG8EF_000557"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS4945908.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACG8EF_000558"
     CDS             16546..17946
                     /locus_tag="ACG8EF_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFS4945909.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACG8EF_000559"
     CDS             complement(18024..18746)
                     /locus_tag="ACG8EF_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4945910.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACG8EF_000560"
     CDS             complement(18825..19331)
                     /locus_tag="ACG8EF_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4945911.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACG8EF_000561"
     CDS             19454..21253
                     /locus_tag="ACG8EF_000561"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFS4945912.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ACG8EF_000562"
     CDS             complement(21321..22895)
                     /locus_tag="ACG8EF_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS4945913.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACG8EF_000563"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACG8EF_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFS4945914.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACG8EF_000564"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACG8EF_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFS4945915.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="ACG8EF_000565"
     CDS             complement(24672..25310)
                     /locus_tag="ACG8EF_000565"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFS4945916.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACG8EF_000566"
     CDS             complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACG8EF_000566"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS4945917.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACG8EF_000567"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACG8EF_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFS4945918.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACG8EF_000568"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACG8EF_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFS4945919.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="ACG8EF_000569"
     CDS             complement(29104..29844)
                     /locus_tag="ACG8EF_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFS4945920.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACG8EF_000570"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACG8EF_000570"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFS4945921.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACG8EF_000571"
     CDS             complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACG8EF_000571"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFS4945922.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACG8EF_000572"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACG8EF_000572"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS4945923.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACG8EF_000573"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACG8EF_000573"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFS4945924.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACG8EF_000574"
     CDS             complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACG8EF_000574"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4945925.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACG8EF_000575"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACG8EF_000575"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS4945926.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="ACG8EF_000576"
     CDS             complement(36724..37008)
                     /locus_tag="ACG8EF_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945927.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="ACG8EF_000577"
     CDS             complement(37026..37916)
                     /locus_tag="ACG8EF_000577"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFS4945928.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="ACG8EF_000578"
     CDS             complement(37936..38154)
                     /locus_tag="ACG8EF_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFS4945929.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="ACG8EF_000579"
     CDS             complement(38124..39824)
                     /locus_tag="ACG8EF_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFS4945930.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="ACG8EF_000580"
     CDS             40091..40864
                     /locus_tag="ACG8EF_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945931.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="ACG8EF_000581"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="ACG8EF_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFS4945932.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="ACG8EF_000582"
     CDS             complement(41688..42659)
                     /locus_tag="ACG8EF_000582"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS4945933.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="ACG8EF_000583"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="ACG8EF_000583"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFS4945934.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="ACG8EF_000584"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="ACG8EF_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFS4945935.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
BASE COUNT        12396 a         9875 c         8235 g        15006 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cg
//
LOCUS       DAZUKZ010000011        44939 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000011 DAZUKZ010000000
VERSION     DAZUKZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.011"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..1189)
                     /locus_tag="ACG8EF_000585"
     CDS             complement(38..1189)
                     /locus_tag="ACG8EF_000585"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS4945936.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="ACG8EF_000586"
     CDS             complement(1202..1639)
                     /locus_tag="ACG8EF_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4945937.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="ACG8EF_000587"
     CDS             complement(1654..3420)
                     /locus_tag="ACG8EF_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFS4945938.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="ACG8EF_000588"
     CDS             3684..4361
                     /locus_tag="ACG8EF_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4945939.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="ACG8EF_000589"
     CDS             4364..5491
                     /locus_tag="ACG8EF_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4945940.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="ACG8EF_000590"
     CDS             complement(5691..6131)
                     /locus_tag="ACG8EF_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4945941.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="ACG8EF_000591"
     CDS             complement(6142..8175)
                     /locus_tag="ACG8EF_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFS4945942.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="ACG8EF_000592"
     CDS             complement(8177..9880)
                     /locus_tag="ACG8EF_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4945943.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACG8EF_000593"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACG8EF_000593"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFS4945944.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="ACG8EF_000594"
     CDS             complement(10963..11988)
                     /locus_tag="ACG8EF_000594"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4945945.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="ACG8EF_000595"
     CDS             complement(12197..13066)
                     /locus_tag="ACG8EF_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4945946.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="ACG8EF_000596"
     CDS             complement(13525..15417)
                     /locus_tag="ACG8EF_000596"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4945947.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="ACG8EF_000597"
     CDS             complement(15414..17141)
                     /locus_tag="ACG8EF_000597"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4945948.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="ACG8EF_000598"
     CDS             complement(17157..17807)
                     /locus_tag="ACG8EF_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFS4945949.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="ACG8EF_000599"
     CDS             18011..18307
                     /locus_tag="ACG8EF_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4945950.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="ACG8EF_000600"
     CDS             18479..19099
                     /locus_tag="ACG8EF_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS4945951.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="ACG8EF_000601"
     CDS             complement(19191..20357)
                     /locus_tag="ACG8EF_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4945952.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="ACG8EF_000602"
     CDS             complement(20369..20722)
                     /locus_tag="ACG8EF_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS4945953.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="ACG8EF_000603"
     CDS             20850..21872
                     /locus_tag="ACG8EF_000603"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFS4945954.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="ACG8EF_000604"
     CDS             21891..22052
                     /locus_tag="ACG8EF_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945955.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="ACG8EF_000605"
     CDS             complement(22311..22745)
                     /locus_tag="ACG8EF_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945956.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="ACG8EF_000606"
     CDS             complement(22855..24714)
                     /locus_tag="ACG8EF_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4945957.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            24715..24855
                     /locus_tag="ACG8EF_000607"
     CDS             24715..24855
                     /locus_tag="ACG8EF_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFS4945958.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(24832..25992)
                     /locus_tag="ACG8EF_000608"
     CDS             complement(24832..25992)
                     /locus_tag="ACG8EF_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4945959.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACG8EF_000609"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACG8EF_000609"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFS4945960.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="ACG8EF_000610"
     CDS             complement(27739..28437)
                     /locus_tag="ACG8EF_000610"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS4945961.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="ACG8EF_000611"
     CDS             complement(28394..30055)
                     /locus_tag="ACG8EF_000611"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFS4945962.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="ACG8EF_000612"
     CDS             30273..31673
                     /locus_tag="ACG8EF_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFS4945963.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="ACG8EF_000613"
     CDS             complement(31925..33004)
                     /locus_tag="ACG8EF_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4945964.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="ACG8EF_000614"
     CDS             complement(33090..34049)
                     /locus_tag="ACG8EF_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS4945965.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="ACG8EF_000615"
     CDS             complement(34061..35572)
                     /locus_tag="ACG8EF_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFS4945966.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="ACG8EF_000616"
     CDS             complement(35594..36157)
                     /locus_tag="ACG8EF_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4945967.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="ACG8EF_000617"
     CDS             complement(36163..37026)
                     /locus_tag="ACG8EF_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4945968.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="ACG8EF_000618"
     CDS             complement(37040..37936)
                     /locus_tag="ACG8EF_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4945969.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="ACG8EF_000619"
     CDS             complement(38111..39400)
                     /locus_tag="ACG8EF_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4945970.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="ACG8EF_000620"
     CDS             complement(39372..40889)
                     /locus_tag="ACG8EF_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFS4945971.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="ACG8EF_000621"
     CDS             41087..41977
                     /locus_tag="ACG8EF_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFS4945972.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ACG8EF_000622"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ACG8EF_000622"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFS4945973.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="ACG8EF_000623"
     CDS             complement(42886..44358)
                     /locus_tag="ACG8EF_000623"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFS4945974.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT        12989 a         9387 c         7783 g        14780 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgttttt
//
LOCUS       DAZUKZ010000012        44836 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000012 DAZUKZ010000000
VERSION     DAZUKZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.012"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG8EF_000624"
     CDS             212..604
                     /locus_tag="ACG8EF_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4945975.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG8EF_000625"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG8EF_000625"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFS4945976.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG8EF_000626"
     CDS             2698..2943
                     /locus_tag="ACG8EF_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945977.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG8EF_000627"
     CDS             3165..4775
                     /locus_tag="ACG8EF_000627"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFS4945978.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG8EF_000628"
     CDS             complement(4752..4964)
                     /locus_tag="ACG8EF_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945979.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG8EF_000629"
     CDS             5067..5936
                     /locus_tag="ACG8EF_000629"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFS4945980.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG8EF_000630"
     CDS             6101..7372
                     /locus_tag="ACG8EF_000630"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS4945981.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG8EF_000631"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG8EF_000631"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFS4945982.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG8EF_000632"
     CDS             8763..9026
                     /locus_tag="ACG8EF_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFS4945983.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG8EF_000633"
     CDS             9471..9920
                     /locus_tag="ACG8EF_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4945984.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG8EF_000634"
     CDS             9977..11395
                     /locus_tag="ACG8EF_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS4945985.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG8EF_000635"
     CDS             11395..12255
                     /locus_tag="ACG8EF_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS4945986.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG8EF_000636"
     CDS             12429..13202
                     /locus_tag="ACG8EF_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4945987.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG8EF_000637"
     CDS             13349..13714
                     /locus_tag="ACG8EF_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFS4945988.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG8EF_000638"
     CDS             complement(13862..15070)
                     /locus_tag="ACG8EF_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4945989.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG8EF_000639"
     CDS             complement(15089..15367)
                     /locus_tag="ACG8EF_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4945990.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG8EF_000640"
     CDS             15560..16216
                     /locus_tag="ACG8EF_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4945991.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG8EF_000641"
     CDS             complement(16658..17455)
                     /locus_tag="ACG8EF_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFS4945992.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG8EF_000642"
     CDS             complement(17452..18636)
                     /locus_tag="ACG8EF_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFS4945993.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG8EF_000643"
     CDS             complement(18661..19908)
                     /locus_tag="ACG8EF_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS4945994.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8EF_000644"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8EF_000644"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFS4945995.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG8EF_000645"
     CDS             21888..22202
                     /locus_tag="ACG8EF_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFS4945996.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG8EF_000646"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG8EF_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFS4945997.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8EF_000647"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8EF_000647"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS4945998.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG8EF_000648"
     CDS             23546..23875
                     /locus_tag="ACG8EF_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFS4945999.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG8EF_000649"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG8EF_000649"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFS4946000.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG8EF_000650"
     CDS             24863..25687
                     /locus_tag="ACG8EF_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFS4946001.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG8EF_000651"
     CDS             25680..26027
                     /locus_tag="ACG8EF_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFS4946002.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8EF_000652"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8EF_000652"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFS4946003.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8EF_000653"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8EF_000653"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFS4946004.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8EF_000654"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8EF_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFS4946005.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8EF_000655"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8EF_000655"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFS4946006.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACG8EF_000656"
     CDS             31684..32451
                     /locus_tag="ACG8EF_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4946007.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACG8EF_000657"
     CDS             32441..34483
                     /locus_tag="ACG8EF_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4946008.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="ACG8EF_000658"
     CDS             34764..34913
                     /locus_tag="ACG8EF_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFS4946009.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="ACG8EF_000659"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="ACG8EF_000659"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFS4946010.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="ACG8EF_000660"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="ACG8EF_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFS4946011.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="ACG8EF_000661"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="ACG8EF_000661"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFS4946012.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="ACG8EF_000662"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="ACG8EF_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFS4946013.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="ACG8EF_000663"
     CDS             39330..40412
                     /locus_tag="ACG8EF_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFS4946014.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="ACG8EF_000664"
     CDS             40412..41215
                     /locus_tag="ACG8EF_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS4946015.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="ACG8EF_000665"
     CDS             41285..42025
                     /locus_tag="ACG8EF_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4946016.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="ACG8EF_000666"
     CDS             42036..43040
                     /locus_tag="ACG8EF_000666"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFS4946017.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..>44836)
                     /locus_tag="ACG8EF_000667"
     CDS             complement(43085..>44836)
                     /locus_tag="ACG8EF_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946018.1"
                     /translation="TGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDSKYTR
                     TIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGL
                     SLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMI
                     IAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHS
                     FLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELM
                     PIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTT
                     INWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEV
                     NTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARY
                     QKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSI
                     HEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDT
                     FIDRITVQLSRNYPNPV"
BASE COUNT        14406 a         7826 c         9341 g        13263 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt agaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgt
//
LOCUS       DAZUKZ010000013        43643 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000013 DAZUKZ010000000
VERSION     DAZUKZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.013"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(283..2664)
                     /locus_tag="ACG8EF_000668"
     CDS             complement(283..2664)
                     /locus_tag="ACG8EF_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS4946019.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2654..3301)
                     /gene="recU"
                     /locus_tag="ACG8EF_000669"
     CDS             complement(2654..3301)
                     /gene="recU"
                     /locus_tag="ACG8EF_000669"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFS4946020.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3365..3904
                     /locus_tag="ACG8EF_000670"
     CDS             3365..3904
                     /locus_tag="ACG8EF_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFS4946021.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3994..4413
                     /gene="gpsB"
                     /locus_tag="ACG8EF_000671"
     CDS             3994..4413
                     /gene="gpsB"
                     /locus_tag="ACG8EF_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFS4946022.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4435..4802
                     /gene="rnpB"
                     /locus_tag="ACG8EF_000672"
     ncRNA           4435..4802
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8EF_000672"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5011..6177
                     /locus_tag="ACG8EF_000673"
     CDS             5011..6177
                     /locus_tag="ACG8EF_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFS4946023.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6223..7719
                     /locus_tag="ACG8EF_000674"
     CDS             6223..7719
                     /locus_tag="ACG8EF_000674"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFS4946024.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7800..8483
                     /locus_tag="ACG8EF_000675"
     CDS             7800..8483
                     /locus_tag="ACG8EF_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4946025.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9128..10318
                     /locus_tag="ACG8EF_000676"
     CDS             9128..10318
                     /locus_tag="ACG8EF_000676"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFS4946026.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10420..11373
                     /locus_tag="ACG8EF_000677"
     CDS             10420..11373
                     /locus_tag="ACG8EF_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFS4946027.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11442..12791
                     /locus_tag="ACG8EF_000678"
     CDS             11442..12791
                     /locus_tag="ACG8EF_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4946028.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12974..13297)
                     /locus_tag="ACG8EF_000679"
     CDS             complement(12974..13297)
                     /locus_tag="ACG8EF_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946029.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13426..14589
                     /locus_tag="ACG8EF_000680"
     CDS             13426..14589
                     /locus_tag="ACG8EF_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4946030.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14659..16008
                     /locus_tag="ACG8EF_000681"
     CDS             14659..16008
                     /locus_tag="ACG8EF_000681"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4946031.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16053..16484)
                     /locus_tag="ACG8EF_000682"
     CDS             complement(16053..16484)
                     /locus_tag="ACG8EF_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4946032.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16872..19514
                     /gene="alaS"
                     /locus_tag="ACG8EF_000683"
     CDS             16872..19514
                     /gene="alaS"
                     /locus_tag="ACG8EF_000683"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFS4946033.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19714..20310
                     /locus_tag="ACG8EF_000684"
     CDS             19714..20310
                     /locus_tag="ACG8EF_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4946034.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20432..21580
                     /locus_tag="ACG8EF_000685"
     CDS             20432..21580
                     /locus_tag="ACG8EF_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4946035.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21618..22031)
                     /locus_tag="ACG8EF_000686"
     CDS             complement(21618..22031)
                     /locus_tag="ACG8EF_000686"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFS4946036.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22110..22541
                     /locus_tag="ACG8EF_000687"
     CDS             22110..22541
                     /locus_tag="ACG8EF_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFS4946037.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22640..22873)
                     /locus_tag="ACG8EF_000688"
     CDS             complement(22640..22873)
                     /locus_tag="ACG8EF_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4946038.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23056..24726
                     /locus_tag="ACG8EF_000689"
     CDS             23056..24726
                     /locus_tag="ACG8EF_000689"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFS4946039.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24873..25259
                     /locus_tag="ACG8EF_000690"
     CDS             24873..25259
                     /locus_tag="ACG8EF_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946040.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25246..25944
                     /locus_tag="ACG8EF_000691"
     CDS             25246..25944
                     /locus_tag="ACG8EF_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFS4946041.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25949..26413
                     /gene="lspA"
                     /locus_tag="ACG8EF_000692"
     CDS             25949..26413
                     /gene="lspA"
                     /locus_tag="ACG8EF_000692"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFS4946042.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26410..27321
                     /locus_tag="ACG8EF_000693"
     CDS             26410..27321
                     /locus_tag="ACG8EF_000693"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4946043.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27556..28095
                     /gene="pyrR"
                     /locus_tag="ACG8EF_000694"
     CDS             27556..28095
                     /gene="pyrR"
                     /locus_tag="ACG8EF_000694"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFS4946044.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28109..29392
                     /locus_tag="ACG8EF_000695"
     CDS             28109..29392
                     /locus_tag="ACG8EF_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFS4946045.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29405..30331
                     /locus_tag="ACG8EF_000696"
     CDS             29405..30331
                     /locus_tag="ACG8EF_000696"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFS4946046.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30350..31630
                     /locus_tag="ACG8EF_000697"
     CDS             30350..31630
                     /locus_tag="ACG8EF_000697"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFS4946047.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31635..32711
                     /locus_tag="ACG8EF_000698"
     CDS             31635..32711
                     /locus_tag="ACG8EF_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFS4946048.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32716..35901
                     /gene="carB"
                     /locus_tag="ACG8EF_000699"
     CDS             32716..35901
                     /gene="carB"
                     /locus_tag="ACG8EF_000699"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFS4946049.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35955..36731
                     /locus_tag="ACG8EF_000700"
     CDS             35955..36731
                     /locus_tag="ACG8EF_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFS4946050.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36728..37660
                     /locus_tag="ACG8EF_000701"
     CDS             36728..37660
                     /locus_tag="ACG8EF_000701"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFS4946051.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37657..38373
                     /gene="pyrF"
                     /locus_tag="ACG8EF_000702"
     CDS             37657..38373
                     /gene="pyrF"
                     /locus_tag="ACG8EF_000702"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFS4946052.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38380..39006
                     /gene="pyrE"
                     /locus_tag="ACG8EF_000703"
                     /pseudo
     CDS             38380..39006
                     /gene="pyrE"
                     /locus_tag="ACG8EF_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            39033..39311
                     /locus_tag="ACG8EF_000704"
     CDS             39033..39311
                     /locus_tag="ACG8EF_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946053.1"
                     /translation="MRFVSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFI
                     FLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39349..39990
                     /locus_tag="ACG8EF_000705"
     CDS             39349..39990
                     /locus_tag="ACG8EF_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFS4946054.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40033..40725
                     /locus_tag="ACG8EF_000706"
     CDS             40033..40725
                     /locus_tag="ACG8EF_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFS4946055.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40722..41624
                     /locus_tag="ACG8EF_000707"
     CDS             40722..41624
                     /locus_tag="ACG8EF_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4946056.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41782..43488)
                     /gene="efbA"
                     /locus_tag="ACG8EF_000708"
     CDS             complement(41782..43488)
                     /gene="efbA"
                     /locus_tag="ACG8EF_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFS4946057.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13981 a         7691 c         9487 g        12484 t
ORIGIN      
        1 atttgataat aacaatggga ttattaagaa gtggcactaa ataaaagact agtgtgtatt
       61 tagccgattt aattataaac aagtaaatgg ataaagtata tgtaatgata aaagaaaaat
      121 ggattttcta atttttaagc taaatgttag atcacacatg tatatgctgg atagtcttat
      181 aaaaaatcat gtaatacagc ttggtacatg atttttattt gtagctgttt tttacataga
      241 cacaaaaaag aaaccacgct tgtagacgtg gtttcttttt tactatctgt ttccagaagc
      301 tgaggtgctg ctggatggtg tatggtcatt tgcagcatcc gctccgctag ttgctggctc
      361 actggaagaa gcaggaggag cagatgaaga cggtgtcgag ctttcttcgg atgaagaaga
      421 aggaaccgtt gttgaagacg ctggctcgct tgtttctgct gtcgtgcttt cttttgagga
      481 ctcagcagtt gattcgcttt gactagtaga gctagagctt tgagtcgttg tctctgttgt
      541 tgagcttcca ggagtctgga catagctgga agatggaata gttgttgatg gtgtaattgc
      601 attcgatcta gctgtatatt gatctttgta gtacagttca ccgcccacac gaattaatcc
      661 gcttggcatc tcccagtctg tatttgtcac gttagctgaa acatactgca taagctcgcg
      721 ataaacatca gaggctacgt gtgacgattc tgaagtgaca ggtgtcattt ttttattgta
      781 acctgtccac acggaaatcg aataatttgg cgtatagccc gcaaatagga tatctggata
      841 caccccagaa gaaataccaa gttttgcata ctcgtcatct gtgtaattgg atgttccggt
      901 cttaccggct tgatataacc cagcgatttg cgcattcgta ccagttcctt cagtgatcgt
      961 atcttttaaa atgtctgtga tcatataagc tgtttctgga gacatggctg tttttccatc
     1021 tggctcataa gtttcttctg ttccgtcttg gaagacgatt ttattaacat attgaggttt
     1081 gtaataagtc cctccattgg cgaaagccgc ataggctgct gccattttta aggaagaagc
     1141 tccgtatttt gtcccatctt gttcttccgt attactagag atcgcattcg attgatggat
     1201 cgtactgtat tcgatcccaa gattttttaa gaaagaagcc actttatctg aaccgacttc
     1261 attgaataat ttgactgctg gaacattacg ggataagtaa agtgcttggc gtaaagtaat
     1321 ggtgcccata tattgattat cccagtttcc tacaggagtc gaagtccctt catagttata
     1381 cggtgcgtca gtaatagttt ttccagtaga atatttcaaa tattcaaaag ctggtccgta
     1441 atctgtgaca ggtttcatcg tagaaccaaa atcacgggaa gtattcactg ctaagttgtt
     1501 gcctaaagtt acgtcttctg cgatgtgacg tccgccaatc tgggctttta ctttcccggt
     1561 attggtgtcg ataagggtgg aggcaacttg catctcgtcg tctgggtaag agacatactg
     1621 atccgtgttg acgatatcat atagtttctt ttgggcgtcc agatccaaat tcgtataaat
     1681 ctctaaaccg tctgtgtaga cattcttatc tgttttttct tgtacttcat tgatgacttc
     1741 tttgacataa ttatcgacga tcttagtatt gtcatcggat tgagttagtt cttgcagacc
     1801 atccgttacc ggtacattaa cagcttgatc atattctgtt tgagagattt tttcgttttg
     1861 aagcatcgta tacaaaacag tatctcgtct tttcttcgcc tgatctggat aaacataagg
     1921 atcataggcg gaaggggctt gaggcatccc agcaagcaat gctgtttgcg gtaaagaaag
     1981 ttctgataat ttcttaccaa agtacatctc agaagctgtc tccatcccgt acaagccgtt
     2041 tgacatatac actttattta cgtagtaggt caagatctct tgtttcgatt ttttttgttc
     2101 caatcttaca gccatccacg cttcttgggc cttgcgtttc agagtctgat cctcggcact
     2161 ggtggagaag aaggaaagtt tgatcaattg ctgtgtcaaa gtacttccgc cttgcagtcc
     2221 gccagaggta aagttagaga gggcagaacc gatgatccga atagggtcaa ccccgatgtg
     2281 tttataaaaa cggcgatctt ctaccgacac gatcgcatct tctaatgttt tcggtaattc
     2341 attcgcagaa attttttcac gcttttctgc gcctaaatct tcgaatagtt cgccatcttg
     2401 tgtatatagt ttggaagata cggtagcgtc taatttttta tccgtcaatt cgggcgcatc
     2461 ttttgcgtaa taccagaaaa gtcctacacc tgctaaaaag aggatacaga aaaatgagag
     2521 tattcctaaa aggatcttga taaataatcc ttttttatgt gttccagagg actttccaga
     2581 acctttgcca gaattcttct tcacactctt tttaggagta ggctgtttct gtcgtctaga
     2641 gcttctagtt tgttcatttg ccattcttgc tttctccttc aatagataat gttgataaat
     2701 agaagtcaac cgcgtccaaa taaggaatcc gcggagaaat accagttttg atctctattc
     2761 ctagttcttc gatcaaagat aaaggtagtg atttgcgtcc tttttccttt tgggcgtccc
     2821 agtaagcaat caaatgaaca ctattcaaat aaaaacagcg cttcaaagac gaaaaccata
     2881 acaagacaaa gcagacccca ttttgttcga ggcatgcttt catatgttcg atttgatggg
     2941 catggaagtt tttaaaggga aaagatgttt tgttcttggt ttcttttgct tcaaagtcca
     3001 agtaaaagcc tcgatagacg ccattatagt ctgtagttga ggcttgggta aagtaggctt
     3061 ccttgatcac agcagcactt ctcttaggat agtctacttt cacgatttgg ataggggtag
     3121 gctttttatg gataaccgcc aacccgcggg aaagataata tttattactt tcattgattg
     3181 cttcttcaaa gcgcatcccc cgattgccga attcagtgat ctgatttctt ttttcttgaa
     3241 ttttttcgac gggagaggca ttcttataat aaggttggcc atttggatag cgcaatacca
     3301 tctttaatca cactcctagg ccacattata gcaaaaaagc ggatagaaag aaaaggaatc
     3361 tgagatggat aagtcaaaag taatctatgt gtctgggtat cgaagtttcg aactcggtgt
     3421 ttttaaagaa aatgatccta aaatccaagt tgttaaaaat gttttaaaaa aagaactcca
     3481 tcagttagca gaagaagggc tggaatgggt acttgtcagt gggaatttgg gcgtcgaact
     3541 atgggctgtg caaatagtag cggaattgaa agaagaatat ccggaactat ctcttggtat
     3601 tttgtatcct ttttctgatt ttggtaataa ctggaatgaa gggaaccaag aaaaaaaaca
     3661 attagcagaa caattagcag attatgtcga agctgttagc catcaaccct accaatcacc
     3721 tagtcagcta aagaatcaca ctcgttttat acttgagcat acagtgggct gtctgctgat
     3781 ctatgatgaa gaattccctg gaaaaacgaa gtattttttt gaagatgcac agaattacca
     3841 agcagatcat ccctacgaaa ttcggctgat cagtatggac gatcttcaaa attttagtga
     3901 ataatcgata ttgtgtttga tttatcaact tttttcctat acaatgaaaa aggtagaaat
     3961 gttattacgt aaatgaaaac tagaggtgta aatatggcga atttggttta tagtcctaaa
     4021 gacattttac aacaagaatt caaaacaaaa atgcgcggct atgatcccgt tgaagttgat
     4081 gaattcttag acaatatcat caaagattat gaagcttaca gcaaagaact ccttgcttta
     4141 caagaagaaa acgatcgatt gagtgcaaaa gtagctcaat tatctaaaac tcaaggagca
     4201 gctcaaacac gcgtgcaaca aacagaggca ccaaagagcg cagcagtgac caattttgat
     4261 atcttaaaac gcttgtcaaa tcttgaacgt gaagtatttg gtaaaaagct tgatcaacaa
     4321 gcaagtgcag tcaaaccagc acaacctaat ccaaataact acacaaatgc agacacaagc
     4381 ttagatgata atgaaaaaac acgtcaattt taatttttga ttaatgaatt tgcggttttc
     4441 gggtaatcgc ggtctgactt gatcaggctg aggaaagtcc atgctcgcac aggctgtgat
     4501 gcctgtagtg ttcgtgctta gcgaaaccat aagctaaggt actctttgga gtaacggcag
     4561 gaaaacaagc taaggcttcg gctatgctta agtatccttg aaagtgccac agtgacgaag
     4621 cggctgggga aacccatccg gtggaacgcg gtaaacccct cgagcgagca acccaaacaa
     4681 tggtaggggc gctttttgtc tggaagaaga acaggacaaa aaggcagtat aactgcagat
     4741 agatggttac cgctttgttt tttctccctg gaaaagcaaa gtacagaaca tggcttatag
     4801 aaaaccgcaa acatcaggga attaggtcgt gacaaaagta atgcgttata agaaacaaga
     4861 aaaacatttg aaagctattt ttcaatactt cagatgaatc gttcttatta caaaacaaac
     4921 ttttgtatca ccggttattc ggaatttgag taaaagtgtg gggtaggact gatgtcccac
     4981 ctcctttttt tataaataag gagattgtgt atggaaaaaa acaggaagtt caacctcgtc
     5041 gcaactgcgg caagcgggct agaagcactg gttggtaaag aattaagaga tcttggaatc
     5101 gaatgccaag ttgagaacgg acgtgcacgt tttgaaggaa caatggaaac gatcgctaca
     5161 gctaatttat ggttacgtac agcagatcga gtaaaaatcg tggttgggga attcgatgcg
     5221 tatacgtttg atgaattgtt tgaaaacgta aaagcattgc cttgggaaga ttttcttcca
     5281 ctagatgcag cttttcctgt agctggaaaa tcgatcaagt cgaaactata tagcacacct
     5341 gattgccaag cgatcacaaa aaaagcaatc gttgaacgtc tccgtaccta ttatcaccgt
     5401 ccagcttctg taccactgac ggaaactggc gcacattttc agttggaagt agctttgctg
     5461 aaagaccatg tgatggtaac tctggatacg acaggaccaa gtttattcaa acgtggttat
     5521 cgcttagaaa aaggtggagc accattgaaa gaaaacatgg cagcagcact tattatgttg
     5581 acaaattggc gaaaagaccg tccattttat gatcctgtat gtggttcagg tacgctttgt
     5641 attgaagcag ccttaatagg ccataatatc gcaccaggat tcaatcgtga gtttgcttgc
     5701 gaatcttggg attggttcag tcaagaaatc atggaaaatg ttcgatcagc agccgaagaa
     5761 aaagcagatt atgatattga attagatatt actggttcag atatcaatgg acggatgatt
     5821 gaaattgcca aagccaatgc agaagaaatc ggattaggct cttcgattac ctttaagcaa
     5881 caagcagtca aagacttcaa aacggataaa gaatacgggg tcatcgtggc taatccgcct
     5941 tacggagaac gtctaggaga agaagaaact gttcggaaat tatataaaga aatgggcgat
     6001 gtattccgtc cattaaaaac atggagtaaa tatatcttga ctagcgactt agcgtttgag
     6061 gaattttacg gacaaaaagc aaccaaaaaa agaaaattgt acaatggtgc attgcgtacg
     6121 gatctattcc agtattgggg aactcgtccg cctagaaaaa aagagaaaga cgaataatct
     6181 tactaattga ttcattcaat tggatgtttg gaggaatagg gaatgaaaga aaaagagttt
     6241 ttacaagaag taaaagaaat caatcttcta caacaagctt taggaatcct cgattgggat
     6301 acacaaacag gtatgcctga aaaagctagt gcataccgaa gtgaagtaga cagctatctt
     6361 tacagcattt atttttcaaa aaaaatcggt ccaaaaatca aagaagccat tcgctatttt
     6421 tctgaacatc cggaagaatt gtcggaagtt ggaactgccg tttttgccaa agtaaaagaa
     6481 gagtatgatt tggaacatcg cgtaccggaa gatcttatga gaaaatatac agctgccgtt
     6541 tcttcagcac acggacaatg gcaaaaagct agagaaacac aacaattttc tgattttcaa
     6601 cacgcattga atcaaaatat tgaattaaca aaacagttga taccttactg gagaaaagat
     6661 gaaaagacga attatgatgt tttgctgaat caatacgaac ccgggatgac tgtcgaaatc
     6721 cttgataagg tatttgatca gcttaaagaa ggaattcttt cgatccgccg tacgctccaa
     6781 gaaaaaggga ctgaacctga aacagacttt ttaaatcgga gaatgacgaa aaaacaacag
     6841 aagagatttg tagtgaaagt aatcgaacaa cttggttatg attttagtcg tggtcgttta
     6901 gatgacactg tccacccatt tatgactgga attaatccga atgatgcacg aatcacgaca
     6961 cgatggaacg aaacagattt caaaatggct gtttttggag tgatccacga ggctggtcat
     7021 ggtatgtatg aacaagacat cgatgataaa tatgcgtata caccggttta tgaaggtaca
     7081 tcaatgggta tccatgaatc acaatcgttg tttaacgaga tcattattgg aagtagtcgc
     7141 agcttctgga aaaagcaata tcctttcttt caagaatgtg cagaaggaac attcgatgat
     7201 attccttttg acaaatttta tcgttcattg aaccatacaa aatcaagttt ggttcgtatc
     7261 gaggcagaca gtttgactta ccctctccat atcatcattc gttatgaaat cgaaaaaatg
     7321 attttcaatg aaggggtaga tgttgctgaa ctacctgaaa tatggaatca aaaatatgaa
     7381 gagtatctgg gaatacgacc aacaaatgat gtggaaggca tcctacaaga cgttcattgg
     7441 tcaggtgcaa gctttggtta ttttccctct tatgcattag ggtacatgta tgcggcacaa
     7501 ttacttcatt ctttgcagca agaaatagat gtggaaaatg ttctagcgtc agacgattat
     7561 tctccaatca aaaactggat gtcagagcat atccatcaat atggtgcatc tagaaaacca
     7621 aatcaattaa tcatggatgc aacacatgaa ccgcttaatc cgcaatacct gatcgactat
     7681 atgaaagacc tgtattttaa tgtatatcag gtaaaataaa atacaattta caaagatagc
     7741 tgaaattcat ctaatctata gatgttttca gctatttact taagaaagaa gtggaacgaa
     7801 tggataaatt acaagtgatc gctgattata gtatggacca attagcgcat gatcaaacag
     7861 gacatgggag cgatcatacc aaaagagttg tcaagttagc tgaaaaaatt ttggagacgg
     7921 aaccgcaggc agaccgcttt gtgacgcttg cagcagctta cctacatgat acgattgatg
     7981 ataaagtcgt taaaaatgag aacgaggcaa agcaacagct tcgtgtattc cttcgctctt
     8041 tgccaatagt agaggaacag atctcgatga ttttttccat tatcgaaaat atgtcctttt
     8101 ctaaaaatct ctctgaagcg gccgaactat ctctggaagg taagatcgtt caagacgctg
     8161 atcgtatcga agcattagga gcaatcggca ttttgcgtac tgcttatttc ggcggtggac
     8221 atgggcaccc gatttttgat cctgaactgt atccgcaaac attcaaggac aaagaaaatt
     8281 accgaaaagg cacgacagtg atcaatcatt tttatgaaaa actgttcttg ctgtctgata
     8341 aaatgaatac tgattacgga tatgaagaag ccaaacgaag agaaaccttc atgcgtgaat
     8401 tcttagaaga attttttctg gaatggtcta ttgacgatga aaattttact ccagacaatt
     8461 ggatcaaaaa taacaaaaaa tgatggaggt tgtgacagaa gtggtcagct ccaagatatt
     8521 tcggatcatt ttccgtaaaa gttacttctg gaacactgtt taccaagagg tcgtgagcct
     8581 gccgttcagc tccgagtcac aaggaacaat ttcaaaaaat agcttctcgt gtttgatgaa
     8641 tattgtaact tgtgattcgg aattggcagg agaacaccgt ttattcggaa gaataggact
     8701 gtgacaagac ttttgtcaca gtcctttttt cgaattcaac aagtaaaact tttaagaaat
     8761 attcagagag taaaattaaa aattgctgat aaacgaagat ggaaatttta tgtattttta
     8821 ttggtctatg caataaataa attaaatatt cagaatacaa ttgattttaa cgaaagggct
     8881 tctatcaatg gttgtgttgt atttatctgc ccaggtagag taaaatagat cattatctaa
     8941 aaggtaattg ttcatactat ataagtcatt tgaaagtaaa aagatactgt aaataaaaaa
     9001 tgtggttaaa taattctcat aatcagtgta agcgttatca aaaatatagc atacttgctt
     9061 tgtagatgaa tgacacgata tatgtttggt atgttctgca agacagatga atatggaggt
     9121 ggagacaatg tcttccaaag aactaacaat gtatttagag aaagaattgg atcaactgaa
     9181 agaaaagggg ttatacaata cgatcgacat actagaaagt gaaaatggtg cttgtatcat
     9241 cgtagacgga aaaaagatga tcaatctagc atccaacaat tatcttggat ttgccaatcg
     9301 cgaagaattg aaaaaagcat gtattgaagc aaccgaaact tatggtgtag gagcaggagc
     9361 agtccgaacg atcaatggtt cgctgaaaat ccatcagcaa ttagaagaaa aaatcgcaga
     9421 attcaaaggg acagaagcag caatcgcctt tcaatctgga ttcaactgca atatgggcgc
     9481 tatctctgca gtaatgacaa aagaagatgc tatcttatcg gatgaattaa accatgcatc
     9541 tattattgac ggttgccgct tatccggagc aaaaatcatc cgaattaaac atcaagatat
     9601 gaaagacctt gagaaaaaag caaaggaagc gatagaaagt aagaaatata aaaaaatcat
     9661 gtatatcaca gatggtgttt tctccatgga tggagacatt gcacgattgc cggaaattat
     9721 tccgattgtt gaaaaatatg gactgatcac ctacgtcgat gatgcgcacg gctcaggcgt
     9781 aactggaaaa ggtgcaggaa ccgtcaagca ttttggatta agcgacaaaa tcgatatgca
     9841 aatgggcacg ttatcgaaag ctataggtgt tgttgggggt tatgtagctg gatcgaaaac
     9901 actaatcgac tggttgaaag ctagaagtcg gccattccta ttttctactt cgctaactcc
     9961 aggtgcagct gcagctgcat tggcttcgat tactttgatg caagaacatc cggaacttgt
    10021 cgaaaaagtt tgggaaaatg ctaattattt taaagaagaa ttgaaaaaag taggctataa
    10081 catcggtatg tctgaaacac ctattactcc agtcattttg ggagacgaaa aagtgactca
    10141 gactttctct aagaagctga tcgaacatgg gatttatgct aaaccaatcg tttatccaac
    10201 tgtgcctctt gggacaggca ggatcagaaa tatgccgact gcggaacata cgagggaaat
    10261 gcttgatgaa gcagtagcag tttatcaaaa aataggaaaa gaaatggaaa ttatttaact
    10321 aaatttagtt ttttcgtcta tttaaaatta gagaacatgt tcacgcatat attgttaatc
    10381 atctgaagat agtagtaaaa aagatcaggg aggatcatca tgaaaaaagt attggtgaca
    10441 ggatgtttag gacaaattgg ttcagagttg atcacacggc tacgtcaaga actaggcagt
    10501 gaaaatgtca ttgcgacaga tatacgcatg cctgaagtca ttgagaaaaa cgatatcttt
    10561 gagatcctag atgtaatgga ttacgaaaaa atgcgcagca tagcagaaca atatcaagtg
    10621 gacacattga ttcatttggc tgctcttttg tcagctgttg cagaagcaaa accacaatta
    10681 gcctggaatt tgaacgtgac tgggctggtc aatgcattgg aagtcgctag agaattccag
    10741 ctgaaatttt ttacgccaag ttcaatcggc gcgtttggtc caaatactcc aaaagatcat
    10801 accccacaag atactcttca gcgtcctaca accatgtatg gggtaacgaa ggtagctgga
    10861 gaactactat gtgattatta ttatgtgaaa tatggcgtag atactcgtgg tgtccgtttc
    10921 cctggattga tttcttatgc aaccttgcca ggaggcggta cgacagacta cgcagtcgat
    10981 atttattatt cagcaatcaa agaaggaaag tacactagtt tcattgatca aggtacatca
    11041 atggatatga tgtatatgcc agatgcgatc gatgccatta taaaactcat gaatgcagat
    11101 cctgatcgat tggttcatcg aaatgccttc aatatatcgg cgatgtcttt tgacccggaa
    11161 gagatcaaag catctattca aaaaatcata ccagattttg aaatggatta tgatgtggat
    11221 cctgtacgtc agtcaatagc agaatcttgg ccaaattcac tagatgtttc ttgtgcgcaa
    11281 gaggaatggg gcttttcgcc gaaatacgac ttagatgtga tgacaaagga tatgttggaa
    11341 aaactgaaag aaaaattggt agagacggta taaaacaaga accacatcca aaaagttttt
    11401 tatacttttg ggattggcta tggtatgaaa ggggacaagg gatggaagaa tatcaagcca
    11461 cgccagtaaa agaagtaaaa atagaaaatg aattaaaacg tacgatggga ttttttactg
    11521 ctttatcgac tgttatgggc acagtgatcg gtgcaggggt attttttaaa gccgcaagcg
    11581 tagcagaagt tacaggatcc gccagtttgc atatgttttc ttggtttctc ggtggtatga
    11641 tttctgtctg tgcaggacta acaggagctg aactggcagc tgctattcca gaaacaggag
    11701 ggatgattaa atatatcgaa cggatttacg gaaatacagc agctttttta ttgggctggg
    11761 cgcaagtcgt gatttatttt ccagcaaatg tagcagcctt gtcgatcatt ttcggtacac
    11821 agttcgtcaa tctatttggt ctttcccagt caatgattgt tcccgtagct gttacagctg
    11881 ctgtatcgat cctgttgatc aattttttag gttctaaagc tggaggggct tttcagtcga
    11941 ttacgcttgt ctgcaagctt attcctttgt ttgtgatcgt cattttcggt ttgttccgac
    12001 aagaaggagt agacttccaa ctgtttccga tccaagcagg agaaaatctt tcatttttct
    12061 cagccttggg agcgggcttg ctagcaacga tgttcgcata cgacggttgg atacacgtag
    12121 gaaatatttc gggagaattg aaaaagccgg cgaaagattt gccaaaagca atttctttag
    12181 gaatcatcgg aatcatgatc gtttatttat tagtaaatgc ggtgttctta agaaccgcat
    12241 ctatcgacgg agtcgcagga aacagcaatg cggcaagtga cgttgcaaaa atgatttttg
    12301 gcggcttcgg tggaagactg gtcacagttg gtatcttgat ttctgtttat ggaaccatca
    12361 atggctatac ccttactgga atgcgtctcc cttatgttat ggcaaaagaa aacaacctgc
    12421 cttttagcaa gctctttgct aaacttcatg ataaaactaa agttcctgta gccgcaggta
    12481 tcttagagct agtcatcgct attggcatga tgatgatcgg tggttttgat acactaacgg
    12541 atatgctgat ctttgtcatt tggatttttt atacgatggt ctttgttggt gtcattcttc
    12601 ttcgtaaaaa agaacctgat ttgtttcgac catacaaagt tccaatgtat ccgtttattc
    12661 cactagtagc aatcattgga gggactttta tcgtatcaag cacgcttatc actcagacct
    12721 ttttagcttc gatggggata gtattgacac ttgctggtat cccgatttat ctctatttaa
    12781 aacgtagata gctaaaacat tcgaatttta ttagaactgc acttctctgc caaacaaaat
    12841 attttattta aatgaggagt acgtggaaga aatgtcaaaa gtactcgtta attttgatca
    12901 cgtaaatgtc gttttgtcta cgatgaaata gcaaagtaaa agacagaaat cgggctcaac
    12961 cgatttctgt cttttacttt gatttgcgta actgaactat tcggcgtacg ccgcgataaa
    13021 tatttaataa aaccaagcaa ccagaaatga aaatgggaag ccaaatcaga tagacagggt
    13081 agccaagcac tttagacaaa gcatgtggag tcattttatc catttcaaga agtgcttgct
    13141 gccaagtctg aagattgaag actagcatga aacccgaaat aatcaggaga ataaatccta
    13201 aataatgaat tgtagttctt tttcttacat aagcgatcgt agcataaagg aacagtaaaa
    13261 aaataggtac gccaaaaaca aagtacatat tcagcatctc gacaccccct ttgtgtttat
    13321 cttactttac tttgaaagaa atgagaaata atagcctcgt tacttgcttg aaacagcaaa
    13381 ggactgctaa gataagagag atacaaagaa tcaaggagga gtcatatggc aagttatatc
    13441 gaaccgattc gttttgcgct gattgttttt ccatttttag ccttagcgat ctcatctgtt
    13501 ttttttattt atgaatatcg aaagtatgga acatttctag taacaagggc agttattttg
    13561 tattctttcg ttttttattt attgtgtgcg tattttttgg tgatcttacc acttccggca
    13621 aaggagacgg ttgcccaaat gacagggcca aagatggagc tgcatttatt tgcttcatgg
    13681 caacatttca gagacaacac ggtattggtc ttgtctgaac ctagtaccta cctgccggca
    13741 atgaagcaga gtgtattttt ggaaccagct tttaatgttt tattactcat accatttggc
    13801 atttatttaa gatattattt ccggctttct tttatgaaaa cgatgattgc tagtttctgc
    13861 ctttctctat tttttgaatt gacgcagcta agtggacttt attttatcta cccaagacca
    13921 taccgcttat ttgatgtgaa tgatttagtg acaaatacat tgggtggaat cattggtttt
    13981 gtcgttacgc cgttatttac atttatgctt cctacaagaa gacgaatgga tgaactttct
    14041 tatgaaaaag gaaagactgt ctcattgact cgacgcttgg tcgcttattt gatcgactgg
    14101 acatttttat ctcttattaa tttggtagcg gcgattttct ttaaaattgc gttcgattca
    14161 ggggcagtta cttctcatat ctggtggctt ttattagaag taggggtata ctttatgatt
    14221 gtaccgttta taaccaatgg atggacactt ggcaaaaaaa ttgtgcgtat ccgtttggtg
    14281 gaagaagacc gcgaacgaat ttctttaaaa gcattgatga tccgctacgg gtatctgtac
    14341 tttctttttt atggtttaag ctggttgggg cagttgcttc agcaaatcat gaattcgaca
    14401 acgaatacgg tgttgcagat gagtgcagta ttcatttctc tcggtgtctt gttactacaa
    14461 ggtattttta ttttaaatat cttgctatca tttttgagaa aaaaacggat tctgttttat
    14521 gaaaaagcta gtcacacata tactgttagc acgattggcc aaacaaagga tgatgattct
    14581 cgaaattagc tgagcaaaaa cgctttgtct gtgttataat gccaaagtcg tgataattta
    14641 aagagaagag gtgcacgaat gaataatttt gatatattcc gcttcaaacc gtttatcaat
    14701 gaagcgttaa ccgcaaaggg cttcaaacag cccacagagg tccaggaacg tttgattcct
    14761 ttaattgcaa aaggaaaaaa tatcattggg caatctcaaa ctggtacagg aaaaacacat
    14821 acgtttttgt tgcctttgtt tgaaaaaatc aatgcagaaa aagaggaagt acaagttgtt
    14881 attacagctc caagtaggga actagctgca cagatctatg aagcagctac acaaatcgct
    14941 aaattttcta caccggagat ccgtgtctcg aattttgtcg gcggtacaga caaacagcgt
    15001 caattaaatc gtctgcatca ccaacagcca caggttgtag taggcacgcc tggtagaata
    15061 ttagacatga tgaatgaaca agcgctgaag attcacaccg catttgcatt tgttgtcgat
    15121 gaagcggata tgacattgga tatgggtttt ttaaatgaag tagaccaaat tgcaagtcga
    15181 ttaccagaaa agctgcagtt tttagttttt tctgccacta ttccagaaaa acttcgtcca
    15241 ttcttacgaa aatacatgga aaatccaatc attgaagaaa tcaaaccaaa agcagttatt
    15301 tctgaagaca ttgaaaattg gctgatttca acaaaaggta aagatgagaa tcaaatgatt
    15361 tatcaactgc tgacaattgg tcatccttat ttagcaatcg tatttgccaa tacaaaacag
    15421 catgtggatg aaattgccga ttacttgaaa aaccaaggct tgaaagtagc aaagattcat
    15481 ggagacatca ccccaagaga gagaaaacga gtgatgcgac aagtacagaa tctggagtat
    15541 caatatgttg tagcgactga tttagctgct agagggatcg atatcgaagg tgtctctcat
    15601 gttattaatg cagaaattcc agaagatcta gacttcttta tccatcgtgt cggacgtact
    15661 ggacgtaatg gcttaaaagg aacggccatt acgttatatt cgccaaatga tgagcaagcc
    15721 attgatgcga tagaaaaatt gggcgtctca tttgaaccaa aagaaatcaa aaacggggaa
    15781 atcgtcaaaa catacgatcg taatagacgt gccaaacgag aaaaatcaaa agatgtatta
    15841 gatcccacgc tgattggtct tgtgaagaag aaaaagaaaa agatcaagcc tggttataag
    15901 aaaaaaatcg attgggcaat tgcagaaaaa aataaaaaag aacgcaaaat cgaacgcaga
    15961 cagcaaagcc gtactgctcg taaaaataag aaaaattcac ataaataaaa aacaagaggt
    16021 tgtgacaaaa ggcgtgtcac aatctcttgt ttttaagcta gacttttttt cattccgata
    16081 caaagtactt catcttcaat aaaaggttct gtgcaagtat gatacccttg tttttcataa
    16141 aaatctacag ccgtcatttc agctactaca taactagtct gacaaccttc tgaaaccgcc
    16201 cgtttttcag cagcttcaag caactgaccg cctaaccctt ttcctcggta ttcttttgct
    16261 acgcaaaatc gttccggctt gaagcaagta ggtgttttct tttcataacg caaagtagct
    16321 actggtcttt tttgatcaag aaatagataa taggtttgtt gcgggatatc ttttccgtcg
    16381 aattctagtt ctgcaggaat cccttgttct aatacaaaaa cttgatatct caaaaagcag
    16441 ctagctgcgc gtatccattc atccgctcca actatttttt tcatcatatt cctccttgtc
    16501 atgaatagca gagatgtatt gattccttta ttcttatcgt accaaaaatc gtggagtatg
    16561 accaaaagga aatgaaaata aaaattgaga tattgacagt gatcaataga atgcgtatac
    16621 taattaagta cgaaaaggat atgatttgtt ttttaagact cacagaaaat tctttcttgg
    16681 ctgaaaaaag aatagtcact aatcaaattg ctcccttttt ctattttggt agaaataccg
    16741 acgcagctca gcgttatcga gtttaagagg taatgattac acatcattgc aatcaaggtg
    16801 gtaccgcgag ttttcgtcct tggctgtgat ggtgtttttt tgtatttaat ttatagaaag
    16861 caggtaatga catgaaacaa ttaagtagtg cacaagttcg acaaatgttt cttgatttct
    16921 tccaatctaa aggacatacg attgaaccta gtgcgtcttt gatccccgtg aacgatccaa
    16981 ccttattgtg gatcaactct ggtgttgcaa cattgaaaaa atattttgat ggttcagttg
    17041 tgccagaaaa tccaagaatc acaaatgccc aaaaatctat tcgaacaaat gatatcgaaa
    17101 atgtcggtaa aacagcacgg caccatacga tgtttgagat gctgggtaat ttttcgattg
    17161 gtgactattt caaaaatgaa gccatccact gggcatggga atttttgact gatccaaaat
    17221 ggatggcatt cgatccagaa aaactttatg taacagttta tccaaaagat acagaagcaa
    17281 aacgcatctg gcatgaagaa gttggcctgc cactagatca cattattgat gtagaagaca
    17341 acttctggga tatcggtgca ggcccatgtg gtccggatac ggaaattttc tatgaccgtg
    17401 gagaagctta taacgattta gcagaagacg atcctgaaaa ctatccagga ggagaaaatg
    17461 aacgttattt agaaatctgg aacttagtat tttcagaatt caaccataag cctgataaca
    17521 catttgaacc acttcctcat aaaaacattg atacggggat gggattggaa cgcatggtct
    17581 caatcgttca agatgcgtca acgaactttg agacagactt gtttatgcct attatccacg
    17641 ccgtagaaaa gctaagcaaa caagtaaaat atggtgaaaa cccagtaaca gatatttctt
    17701 acaaagtaat cgcagatcat attcgcgccc tttcatttgc tattggcgac ggtgcattgc
    17761 catcgaatga agggagaggg tatgtcttac gccgtttgct tcgtcgtgct gtcatgcatg
    17821 ggaaaaaatt aggtattgaa caagcattct tatatcgttt agtccctgtt gttggggaaa
    17881 tcatggtaag ttattatcct gaagtacttc aacaaaaaga atttattgaa aaagttatcc
    17941 gtacagaaga agaacgtttc aatgaaacaa tcaatgaagg tttggaaatc ttaaatcaag
    18001 tgatccaaga agtaaaaaac gaaaacggga cgactctagc aggaaaagac atcttcaaac
    18061 tatacgacac atatggcttc ccggtagaat taacggaaga ggtggcagaa gatgaaggac
    18121 tgaaagttga tcatgctggt tttgaaaaag aaatggatgc acaacgcgaa cgtgctcgct
    18181 ctgcccgcag tactgaaaaa tcaatggggg tacaatctgc tttattgaca gatatcaaag
    18241 tagaaagtaa attcattgga tacacagaac aagaatcaga tagtcaatta ttggtcatcg
    18301 ttaaaggtga gtctcttttg aatgaagtcg cagaaggtga agctcaaatg atctttgatc
    18361 aaacaccttt ctatgcagaa atgggtgggc aagtagcgga tcaaggatgg atcatgaatg
    18421 aagtaggcga aatcgtagca gttgttacag atgttcaaaa agcgcctaat ggtcaattta
    18481 tgcacaccgt agaagtaaaa gcaccaatga gtgaaggaca aacctatcat ctattgatcg
    18541 atcaatcgat gcgcaatcgg atcatcaaaa accatacagc aacccatcta ctgcacaagg
    18601 cattgaaaga agtacttggc gagcatgcaa atcaagctgg ttctttagtc gctcctggac
    18661 atttacgatt cgactttacc cactttggtc aaatcacttc agatgaatta gctgaaatgg
    18721 aaagaatcgt caacgaaaaa atctgggaag ctatcccagt tgaaacaatt gaaacagata
    18781 tcgatacagc caaaaacatg ggtgcaatgg ctttgtttgg tgaaaaatac ggacatgacg
    18841 ttcgagtagt aaatattggc ggctggtcaa tcgaactgtg cggaggcaca catgtagcaa
    18901 atacagaaaa catcggaatc ttcaaaatcg tttctgaatc aggaattggt gcaggcgtgc
    18961 gacgaatcga ggctgtaaca agtaaagaag cgtacgaact acttgaagaa gaagaaaaac
    19021 aactaaaaca agtagcaaca attgttaaat cacctcaatt gaaagaagtt gtttcaaaag
    19081 cggaacaatt gcaacagcaa gtaagagagc tacaaaagga aaatgaacaa ttagctagca
    19141 agttagcgaa tcaacaagct ggtgatgtat tcaaagatcc agaaacgatc aatggaaaaa
    19201 caatcattgc agcacaagta aaagtgaaag atatgaatca gctgcgtcaa ttagctgacc
    19261 aatggaaaca aaaaaatatt tcagatattc ttgttttagc aaacgaacaa gaaggaaaag
    19321 tcagtctgct agcggcaatg acgaaagaat caaatgaagc tggactaaaa gctggcgact
    19381 tgattaaagc aatcgcaccg aaagtaggcg gtggcggtgg tggacgtcca gacatggcac
    19441 aagctggcgg gaaaaatcct gctggtattc cagatgcact taaagctgcg aaagaatggg
    19501 ttgcgcaaca atagggcgtt tacgtttgac agaaaaacaa aagttcaaac attgaacgaa
    19561 acatgattat gggggcgaag acaaacaagt cttgccctca tacttttttc aattggatgg
    19621 cgcatctaat caaatggtcg gattcggatg gtctttagtt gacgaaagtg acagaaattt
    19681 ttacgattag tataaataaa aaggggtgaa aaaatgaata gtagaaataa gacctttcga
    19741 ctagtcttac gtgcgatctt gttagcaatc atcatcgtac aagcgatggt tccttggctg
    19801 gggtttattc cattaggatt cattagttta acaattatcc atatcacagt aattattgct
    19861 gcagtagtat taggcccaaa agatggcatg gtgattggct tgttttgggg aatcgctaca
    19921 attgttcgtg cctatgccat gccaacaacg ccatttgata cattagtgtt cacgaatccg
    19981 attatctcgg tagttcccag agtacttgtt ggcttagtag caggcttggt tttccactgg
    20041 atttatcaaa aaaataaatc aataacgatc agctcagcct ttgcaggggt actgggttca
    20101 ttggtcaata cagtcttagt gttaggattc atgggattat tttatacagg tgcaacggca
    20161 gatgcctatg gtgtagactc tgctctttta tttaaaacct tagcaggggt tgcagcaatc
    20221 aatgggatac cagaagcaat aggtgcggga atcataacac cattactagc aaaagcgtta
    20281 tttgcagcaa caccgttgaa accagaataa tgaccatctt ttaatagtaa taggacgcac
    20341 gatagataga aaaaggaaga gaggagagga aaacaatcgt cctctccatc tttttacctg
    20401 agcaacaagc aaactagaag gggtaaaaaa cttggctaag aaacaagaag aaaaaaagaa
    20461 acaaataccg caacagcgga caaaaaaagg agtactagcg ggtatcttag gattggttac
    20521 gaatctgatc ttgtttgtct ctaagctaat gatcgggctt ttttcaggaa gtgtctccat
    20581 tatggctgat gcagtgaaca gtctatcaga tactgcttca tccatattga ctttgatcgg
    20641 atttaaaatt gctgcaaagc cagcagatca ggaacatcct tatggacacg aacgcttcga
    20701 atatatcagt gggttatttg tttcgatcat cattacttat gtaggatttc aatttttaga
    20761 gtcttctgct gaaaaaatca tccatccaga aaatgtctct ttaacaccca tcgtcttttt
    20821 tgtcctaatc atttccatct ttttgaaatt tgcacaaggc aaaatgtatc aggttattgc
    20881 gcaatccatt gattctgaaa cattgagagc tacagcaaca gatagttata atgatgtctt
    20941 tactacactg gctgtattgc tttcagcagc aatcgaatgg gtaactggat ggcgtattga
    21001 tggatatata ggttttgctt tagcattata tatcctttat agtggattga cgatgcttcg
    21061 aaattttgta tacgaactat taggaagtcg cccaacgcca gcagagattg aagaaatgga
    21121 agagcattta aatgcttgcg atgatatact tggctatcat gatttattgg tgcataacta
    21181 tggacccaat aaacgctttg catcagtcca catcgaagta gatgaatcaa tgaatttgaa
    21241 tgtggctcat aagattattg atctgataga aaaagacttt aaaaagtctt tggatgtgga
    21301 acttgtctgt cacttggatc caatgcctgt tgataacgaa aaatactata atatcttgcg
    21361 gcagttaaag caaattcttc ttgaaacagg tacagggctt aatattcatg attttagagt
    21421 aattcatcaa ggaaaaacgc tgcaatttga tgtagtggtc cctgagaaat tccagtttgt
    21481 tgatacagaa ctagaaagaa tcttacgtag tcaaatagaa gaaaaaatcg gagattacca
    21541 attggaaatt acgtttgatc acaattatct tttatattaa atgagaaact ataaaaagac
    21601 tccttaaagt ttttaattta aagagtcttt ttttctttta attgtttatg gagagcctga
    21661 cgtgcaagat tatctgctcc tttatttttg ttttcgggca accattgcag gatcaataaa
    21721 gaaaattcgg atagaagttc ttggattctt tgaaggtagg gaaggaagag ctcgttcttc
    21781 gttgcgtttt tgtcgaccgt agaaactagt gttttgctgt ctgaaaaaca gaaaatcgtt
    21841 tcattctgta agtcgttttt ctttagaaat tctaaagttt ttaaaaagac ttcgaactct
    21901 gcttgatggt ttgtccctat agaaagcggg atagatagct gttcttgttt accttgatag
    21961 acaatcaaga ttccacctcc actaggacca gggttgcctt ttgtagaggc atcaatgtat
    22021 acttttaaca taaaaagaga ttctcctttt tgttcaaact aatgttatgg taagattata
    22081 tcattactga atcggtgaga ggttagagaa tgaaaaaaaa tattcgatgg cagcctgaac
    22141 tggcacatac gatcatctat tggtcattga ccttgatcat tctgtttttt agtctgattc
    22201 tttctttaga aaacacaggt ccttattgga aaagcaattt tgttttaggt atttttcttg
    22261 tttttctatt tctaggtttg cggcgttctt ttattttaaa atcaaactct gtgattatac
    22321 gttatgctgc tttttggaaa gaccgagaaa tatctgtcgg acagattcga tctttgacgc
    22381 caactaatac tggggtgcaa atgatgttgc aagatgagaa agccccatct gttttcatga
    22441 tgcgaaaaaa agatcaagta gtcctttttg atcattttga aaaaaacgga cccaaatcag
    22501 aatcaattcc ttatccttat acgaaaaaaa gaaaaagcta attttaaaaa tcagaaccta
    22561 aaaaaacgaa tttttacagg aaaagaaaag agtgagacgt acggttcatc ccgtttagcg
    22621 tctcactcta tttgtttatc tattcatgca tactagtatc ggtagtctga acgttgcctt
    22681 tcttgacatg ggcagcttgt gttcctcgat tcccttcgat gatctcaaat tcgaccactt
    22741 gattttcttc caaagttttg aatccctctt cttcaatcgc agtaaaatgt acaaaaattt
    22801 cttctgtgtc atcataactg ataaagccgt atcctttttt gttgtcaaac cattttacaa
    22861 ttcctgtggt caaagcactc acgacctttc taaagtctga tcatagcctt cattataaag
    22921 agtatttata agtacgtcaa ataaattaaa aacctgtaat tttatgtttt ttttctgaat
    22981 aaaaaaaggt ataattggca tatcttaaca aagaagatat tttgttgaat aaagttcgtg
    23041 aaaaggggta atcaaatgaa gaatgatctg caaatttcac aagaaacaga gctagaaccg
    23101 attgttgaag tggctagtaa attagatctt aaggaagacg atttggaact gtatgggaaa
    23161 tataaggcga aaatcaattt ttctggaatc aatcgatcaa aggataacgc tgaggggcat
    23221 ttgatcttag ttacttccat caatccaaca ccagctggcg aaggaaagtc aacgatcact
    23281 gtagggttag gagacgcgct caataaaatc aataaaaaat ctgttattgc attgcgagaa
    23341 ccctcacttg gtcctgtgat gggaatcaaa ggtggtgctg caggcggagg atatgcacag
    23401 gtattgccta tggaagatat caacttgcat tttactggtg acatgcatgc tatcacaaca
    23461 gctaataatg cattatctgc tcttttagat aatcatattc atcaaggaaa tgaattgaac
    23521 atcgatgccc gccgagtgat ttggaaacgg gtagtcgatc tcaatgatcg tgaactgcgt
    23581 aaagtaattg ttggcttggg tgggccaatc caaggtgttc caagagaaga tgggtttgat
    23641 atcacagttg ccagcgaaat catggctatc ttgtgtcttg cggcagacct tgaagattta
    23701 aaggctcgtc ttgcaaggat cgttgtcggc tatacatttg accgtcagcc tgtgactgct
    23761 ggagacttga aagcagaagg cgctctggca ttactgctga aagatgcaat caagccgaat
    23821 cttgtacaaa caatttacgg aacaccagct tttgtccacg ggggaccgtt tgccaatatc
    23881 gctcatggct gcaatagtat tttagctacc aagacagcat tgagattagc agattacgtt
    23941 gtcacagagg caggatttgg tgcagatctt ggcggagaga aatttttaga tatcaaagta
    24001 cctaacttga aaaaagcgcc ggatgctgtg gtgattgtcg ccactgtccg tgcattgaaa
    24061 atgcatggcg gaatgaaaaa agatgaactt aaaaatgaga atcttgatgc tttgaaagta
    24121 ggttttgcaa atttaaagcg ccatatacgt aatatggaac aatatcagct acctgtgatc
    24181 gtcgcaatca atgaattcgt aacagataca gacaatgaac tcacactgtt ggagcacttg
    24241 tgtgaaaatc aggggattct tgccaagaga gcgagtgtat gggcagatgg agcagaaggc
    24301 ggcgttgatt tagcaaaagc tgtagtacga ttgattgata gaaaagaagc taattacaag
    24361 ccattatacc gcctagaaga aacgatacaa gaaaagacag aaacaatcgt caagaaaatt
    24421 tacggaggaa atggtgttgt tttcagcaaa aaagcgcaaa aacaaataga agagtttacg
    24481 aagaatggat gggacaaact gccgatttgt atggcaaaaa cacagtattc attttctgat
    24541 gatccttcac tgctaggtgc accggaagat ttcaccatca cgatccgcga aatcatccca
    24601 aaattagggg caggattttt agtagccttg actggagatg ttatgacgat gcctggtttg
    24661 cctaaaaaac ctgctgcatt gaatatggac gtgacaaatg acggagaagt gttgggattg
    24721 ttttaagctt gattataatc taccatcgaa ggagctgtat tttgactaca gcttcttttt
    24781 ttagacgata gggaattaat tttgtttaca ctaggatcat tctttttctt tattgtattt
    24841 tgagctatac taaagcataa taggagggta tgatgaaaac gacatttata agaaaaaccg
    24901 gtttttacgg tatgggtagc tcgattcagc taagaatcaa caatgaaaaa cgcagtgttc
    24961 tcagtcatga acaaaagata acgcttgatc tggaagtgcc ctttacgatg caggtcacct
    25021 tttattggct aaaaagcccc gtttatacag tgaaagaagc aaagcctgct tatgtgatca
    25081 caatgaatta cttgattctt caaatctatc cgtttttatt tctagcaact gggttggggg
    25141 ccgttttcct acaaaattta tttttcagtt tttttgcagt tattgtgttg attgcgttct
    25201 ttttctatat aaaaaaccgg gcatatttga tcaaggagga aaattatggc aaactctgat
    25261 ctttttttaa tcagtttcaa tcgaatcgaa aaatggatga gagacgagat gggaaatgca
    25321 cgaaatatgg gatttaccga acttgtcaga cgcctagctc aaagaaaaca attaatgatc
    25381 agaaaatacg aagatgatct tttacagctt gctcagttaa ggaatgcgat cgtccatgat
    25441 cggatcgcag tcgattttgt cattgcagag ccaaatgatt ggacagtcaa gcgaatccag
    25501 ctgatcgaac aagaactgat tcagcctgaa accgtgctgc ctcgatttgc aaaacgtgta
    25561 acaggttttg aacaagatct tccgttactt gaactgttaa agatagtagc agaaaaaagg
    25621 tattcacaat ttccgttata caataaagga aaatttgtag cattgattac tttacgtaat
    25681 atcgggtttt ggctagcaaa agaaagccaa aaaggtccgg ttgatttaac aaataaacga
    25741 gcgttggatt tgatcatcca aaacggaaag tacacaaatt atcattttgt tccagctaaa
    25801 acacatattt acgaggttga agcgatgttt cgtgagcaag ggactttgga agccgtactt
    25861 atcactaaag atgggaatcc cgatggtaat ctgctcggga tcgtacgacc tcgtgatatt
    25921 tataaacaaa tagaaaagga ttgatttttt gttaattatt tattggatat tcagcgcttt
    25981 gctagtagga gtggatcagt tggtcaaatg gtggatcact gataatctag cgcttggaga
    26041 gacgaaaaat cttataccag gtattttgtc actgaatcat attcgaaaca ctggagccgc
    26101 atggagtatg ctagaaggga agatgtggtt ctttactata gtgacactca ttgcagtcgt
    26161 tgtgattctc acattgatga ttaaaaatcg tgaaaaaggt aatcgctggt tcatgattgg
    26221 attgagtttg attctagctg gtgcgattgg caattttatc gaccgtattc gtcttggcta
    26281 tgtagtagat atgttccaga cagattttat gagctttccg atttttaacg tagcagatgt
    26341 gacacttgta tgtggagtga tttgtatgtt aatttatatt attttagatg aaaaggatca
    26401 aagaaaaaaa tgacagaact taaaacgacg attcaccatg aatctggtag gatcgataaa
    26461 gtattgacta atttattcac cgattacagt cgttcgcaaa ttcagctgtg gctgaaaaat
    26521 ggagcggttt ctgtcaatgg acagccagtc aaagcaaatt acaaagtaaa acaaggagac
    26581 gagattactg tcttgatccc cgaaccagaa acattagatg tggtcccaga aaatatcccg
    26641 ttgacgatcg tttacgaaga tgaagatgta gcggtgataa acaaacctca aggaatggtc
    26701 gttcatcctt cagcaggtca tacgcatggc acaatggtca atgcgctgat gtaccatatc
    26761 aaggatcttt ctacgatcaa cggcgttatt cgtccaggta ttgttcaccg aatcgacaaa
    26821 gatacttccg gactgctaat ggttgccaaa aatgaccagg cacacgaatc attagctcag
    26881 caattaaaag ataagacctc tttgcgcaaa tatgttgctt tagtccatgg agagattcct
    26941 cacgaaaaag gaaccatcaa tgcgccaatc ggccgttcga aggaaaaccg gaaaatgcag
    27001 gcgataagag aagatgggaa gtcggctgtc acacatttta ctgtattgga acgtttcgaa
    27061 ggatttactc tggttgaatt aaaactagaa accggaagaa cacaccagat ccgtgtgcat
    27121 atgaagtata tcggttatcc gttagctgga gatccagtat acggaccgaa gaaaactttg
    27181 aaaggcaatg gtcagtttct ccatgcgcaa atgttaggtt ttacgcaccc gactacaaac
    27241 gaaaagatga tctttgaagc acctcttcca gaagtttttg aaaaaacttt agaaaaatta
    27301 agaaaaaaca ttgcatttta atttagataa cggtatagta attaacaacg aaagcataaa
    27361 acttaataga attcctttaa gtttagtccc gtgaggctaa gaagggtggg agcaaggata
    27421 cgtatggtgc gccataagta gattgctcat tgacatagta tagctatcag tgtatcagta
    27481 tacgctcagt ctaaacattc ctcctgtcct cttagggcag gagttttttt atgcaaaaaa
    27541 gtagggggaa tgaaaatgca agcaaaagaa gtagtagatc aggtaaccat gaagcgagca
    27601 ttgactcgaa ttacttatga gattatcgag agaaaccata gtatccaaga tatcgtattg
    27661 gtaggtatca aaacacgagg aatctacatt gcatcaagaa ttgccgaacg cctgaagcag
    27721 ctagaagata tcgatattcc tgtaggagaa ctggatatca ctttataccg agatgacaaa
    27781 aaagaaaatc cagaagaacc ggaacttcat tcttctgaca tcccagtttc cttagaagga
    27841 aaagaagtta ttttgattga tgatgttttg tatacaggac gaacgattcg agcagcaatg
    27901 gacgcagtaa tggattttgg cagaccaaga aaaatttcat tagcagtcct ggtcgatcgt
    27961 gggcatcgag aattgccaat ccgtgcagat tatgttggga agaacattcc aacatctcgg
    28021 gcggaagaga ttttagtaga gatgcaagag cttgatggac aagatcgcat catgattttg
    28081 aaagaggaag attagaggag agagaaacat gtcggagaaa aaaggatttc ataatgatga
    28141 cgtggtgtta gatatccacg atcgcccaac cccaggacat tgggtaggat taagtttaca
    28201 acacttgttc acgatgttcg gagcaaccgt gttagtcccc atcttagtag gtatcgatcc
    28261 aggtattgcg ctagtcagtt caggactagg tacattggtg tacttattta ctactaaagg
    28321 aaagatacct gcgtacttag gaagcagctt cgctttcatt gcagcgatgc agcttttgat
    28381 gaaaacagat ggttatccag cgattgcaca aggagcagtc acaactggat tagtctattt
    28441 gattgttgcg ctgatcatca aaaagatcgg ttcagcatgg ctggataaaa tcttgcctcc
    28501 aatcgtcgtc ggtccagtca tcatggttat cgggttggga cttgcggcaa atgcagcaac
    28561 aaatgctatg tataatcagg gcacgtatga tttcaagtat attgctgtag cgctgatcac
    28621 attaggatta acaattttct tcaacatgtg gctaagaggt tttctcggat tgattccgat
    28681 tttactaggt attattagcg gatacatcgt agccttgctg tttggaatcg tggatactaa
    28741 gccaatcgta gacgctgcct ggtttgctgt tcctaacttt gagattccct ttgtacaata
    28801 tgaaccaaag ctatatcttg gagcaatcac aacaatggca cccatcgctt ttgtgacgat
    28861 gacagagcat atcggccatc ttatggtatt gaataaacta actaaacgta atttctttga
    28921 agaaccgggt cttcataaga cgttgacagg tgatggatta gcacaaatcg tggcagcctt
    28981 tgtcggaggt cctcctgtaa caagttacgg tgaaaacatt ggtgttctgg ctatcacacg
    29041 tgtccatagc gtgtttgtga tcggtggagc tgcagtattt gcagtcattt taggattcgt
    29101 aggaaaatta agcgctttga ttttaagtat ccctggacca gtcatctcag gaatcagttt
    29161 tatcttattt ggagtaattg ccgcaagtgg aatgaagatt ttaatcgaga acaaaatcga
    29221 ttttgataaa aagaaaaatc tgttgattgc ttcagttatc ttagtagtag gaattggcgg
    29281 actcgtcttt gaagtaggca catttacatt gtcggcaatg gcattagcaa ctgtacttgg
    29341 catcatttta aatcttatct taccagaaac ttcacgaagc gaagaaaact aaggaggaaa
    29401 cattatgatc atcaaatcag aacgtatcag cttaaagcat ttattgaccg ttgaggcact
    29461 tactgatcaa gaagttatgg gcttgatccg cagagggcag gaattcaagc aaggtgctaa
    29521 ttggactccg caaaagcgcc agtactttgc tacaaacctc tttttcgaaa atagtactcg
    29581 cacacataaa agcttcgatg tagcggagaa aaaactaggg ctagaagtga ttgaatttga
    29641 agcaagcaca agctctgtcc aaaaagggga aacactatac gatacagtac tgacgatgtc
    29701 agcattaggc gtagacgtag cggttatccg tcatggtgat gagaactatt atgacgaact
    29761 gatacaaagt aaaacaatcc agtgttcgat tatcaatgga ggagacggaa gcggacagca
    29821 tccaacccaa tgcttgcttg atttgatgac catttatgaa gaatttggtc attttgaagg
    29881 tctgaaagtt gccatcgtag gtgatatcac tcactctcgc gtagctaaat ccaatatgca
    29941 gatgttaaaa cgtctaggcg cacaagtatt cttctcaggt cctagagaat ggtacgacga
    30001 tgaattcgaa gtgtacggac attatatgcc attggatgaa ctactagatc aagttgatgt
    30061 catgatgatg cttcgggtgc aacacgaacg ccatgatgga aaagaaagtt tctcaaaaga
    30121 aggctaccat caggaacatg ggttaacagt tgagcgtgcg aagaagttgc aaaaacatgc
    30181 aatcattatg cacccagcac cagttaaccg tgatgtagaa ttagctgatt cattagtgga
    30241 aggattacag tcgcggatcg ttcagcaaat gagcaatgga gtattcgtca gaatggcaat
    30301 cttagaagca gtgctaaatg gaaaagcgta attataggag gatgaaaaaa tgaaaacact
    30361 cattaaaaac ggtcagatca atacacgaaa aaatatgacg acaccagcag aaatttggat
    30421 agaagatggg cgaatcaaag caatcggtac aggattctca gaagccgaat ttgatgaagt
    30481 gtttgatgca aagggacaat tgatcacacc tggtttagtg gacgtccatg ttcatttgag
    30541 agagccagga tttacataca aggagacgat cgaagctggg actcgctcag ctgctcgtgg
    30601 cggtttcact actgtatgcg cgatgccgaa tttaaatcct gtacctgata cggcagaaaa
    30661 gctgagacaa gtatatgaca tcattagaaa agatgcagtt gtgaaggtat tgcagtatgc
    30721 gccaatcaca gaaaatctgc gaagtgagaa attagttgat caagaagcac tgattgaaga
    30781 aggtgctttt gctttcacaa atgacggtgt aggtgtccaa acagctggga cgatgtatct
    30841 ggctatgaaa gaagctgcaa aaaacaataa agccctagta gcccatacag aagatgaatc
    30901 tttattattt ggcggcgtta tgcacgctgg caagaaggca gaagaacttg gtcttcccgg
    30961 gatcttaagc gtaaccgagt cttctcaaat tgctagagat ctgttgctag ctgaagcaac
    31021 tggtgttcat taccatgttt gccatgtttc tactaaagaa agtgtccgtg taatcagaga
    31081 cgcaaaaaaa gcaggaatcc acgtaacagc agaagtttca ccccatcatc ttattttgat
    31141 cgacgaagat attcccgaag atttcggctt ttggaaaatg aacccgccgc taagaggacg
    31201 ggaggacaga gaagctttga tcgaaggctt gcttgacggt acgatcgatt gcatcgctac
    31261 agaccatgcc ccccatggat tagaagaaaa aagccaaagt ttcatgaaat caccatttgg
    31321 tatcgtagga agcgaaacag cgtttcaact gatctatact cattttgtag aaactggtcg
    31381 attcacgttg gaacaagtga tcaattggtt ggcagttaaa ccggctgaga tttttggttt
    31441 gaatgcagga acgctaacgg ttggtgcccc cgctgatgta gcagtttttg atattacaca
    31501 aacttgcacg atcgataaag aagacttttt atcaaaaggg gagaatacac cttttatcgg
    31561 ctggaaagta aaaggcgaaa cacaaatgac ttttgtcaac ggaaaacttg tgtggcagaa
    31621 gggagaataa aagtatggaa cgtttgctta tattagaaga tggaacagtt ttcgaaggaa
    31681 aggcttttgg tgcagatgct agtgtcttcg gagaaatcgt ttttacaaca agtatgactg
    31741 gttatcaaga aactatcact gatcaaagtt ttaacggtca aatcattaca tttacctatc
    31801 ctatggtggg taattatggg gtaaatagag atgattatga atcgatttct ccaacatgca
    31861 aaggagtagt agtaaaagaa catgcacgtg tagcttcaaa ctggcgaaac caaatgacgc
    31921 tagattcatt tttgaaacaa aaaggaattc ctggaatatc aggtatcgat acgcgcgccc
    31981 ttactcgcaa aatccgtgaa gtcggaacga tgaaagctag tatagttgat gctggagatt
    32041 ccttcgaaca tgcatttgat cagctgaaag caaccgttct agcaaccaat caagtgcagc
    32101 aagtctcaac taacaaacct tatccaagtc caggaacagg tcggaaagtc attgtcattg
    32161 acttcggctt gaaacatagt attttacgag aattgagtaa aagaaactgt aacctcacag
    32221 tacttcccta taacacagat gccgagacga ttttagcgct atcaccagac ggagtcatgc
    32281 tgacgaatgg acctggtgat ccaaaagatg tgccagaagc aatcgaaatg atcaaaaaaa
    32341 tccaaggaaa agtaccgatt tttggtattt gtctcggtca ccagttattt gctttagcca
    32401 acggtgcaga tacctataaa atgaaattcg gacatcgagg attaaatcac ccggtacgag
    32461 aagtagcgac cggaagaatt gattttactt ctcaaaatca tggctatgca gtcgatgaag
    32521 ccacgattga tccagaaaaa ctcatcgtaa ctcatgtaga agtaaatgac ggaacagtag
    32581 aaggagtcaa acacagagat tatcccgctt tcagtgtgca gtttcatcca gatgctgccc
    32641 ctggtccgca tgatgcacta catttatttg atgaatttat ggaaatgatg gacgcaggaa
    32701 aggagcagta aacagatgcc gaaacgtaca gatatcaaga aaattatggt gattggatct
    32761 ggtccgatca tcattggtca agccgcagaa tttgactacg caggaacaca agcctgcctt
    32821 gccttaaaag aagaaggata tgaagtggtt ttagtaaatt ctaatcctgc gacgatcatg
    32881 acggataaag aagtggcaga tcaagtatat atcgaaccga tcacacttga atttgtatca
    32941 aggattcttc gaaaagagcg tccagatgct ctccttccaa ctttaggcgg acagacaggc
    33001 ctcaatatgg caatggagct ggcagcatca ggtattttag aagaattgaa catagaactt
    33061 ctaggaacaa agctttctgc gatcgatcaa gcagaagatc gtgatctatt caaacagttg
    33121 atgaaagaat tgaatcagcc aatccccgag tctgaaatcg tcaatacagt agaagaagct
    33181 gtagattttg caaataaaat cggctatcct gtcatcgttc gtccagcctt tactttaggc
    33241 ggaactggtg gaggtatgtg tgaaaatgaa gaagaattac gtatcgtatc agaaaatgga
    33301 ttaagtcttt cccccgctac ccaatgtttg attgaacgaa gtattgcagg ttttaaagaa
    33361 attgaatatg aagtgatgag agattcggct gataatgcaa tcgttgtctg caatatggaa
    33421 aattttgacc cggtgggtat ccatacaggt gattctatcg tatttgcccc tagtcaaaca
    33481 ttgtctgacc atgaatatca aatgcttcgt gatgcttctc tttcgattat tcgagcactt
    33541 aagatcgagg gaggctgtaa tgtccaactc gctcttgatc cacatagttt taattattat
    33601 gtgattgaag tcaatccgcg tgtttctcgt tcatctgcgt tagcaagtaa ggcgactggc
    33661 tatccgatcg ctaagctagc tgctaaaata gcgcttggat taacattaga cgaaatgaaa
    33721 aatcctgtta caggaacgac ttttgcagaa tttgaacctg cattagatta tgttgtagct
    33781 aaaattcctc gctggccgtt tgataagttt gaaaacggag aacggctttt aggcacgcag
    33841 atgaaagcaa ctggtgaagt gatggctatc ggtcggaata tcgaagagtc tttattgaaa
    33901 gctgtgcgtt ctttagaaat cggtacacac catatcgaac ttcctgagtt ggcagacatc
    33961 acggacgatg acctaatcca aaaagtggtt cgagcacaag atgatcgcct gttttatgta
    34021 gctgaagcta ttagaagagg gtacaccata gaagaattag cggatttgac aaaaatcgat
    34081 ttattctttt tagataaact gttgcatatc gtagaaatcg aacagcagtt gaaacagcag
    34141 ccgttggatg acaagatatt gaaaacagcg aaacaaaacg gttttacaga caaaaaagtg
    34201 gcggagctgt gggaaataaa cgaagaagcc gttcgccaga tgagaaaaga aaatgatttg
    34261 attcctgtat ataagatggt tgacacttgt gcagcggaat tcgaatcaaa aacaccttat
    34321 ttttatagtt cctatgaata tgaaaatgaa agttcacgat cagaaaagcc ctcaattcta
    34381 gtactgggat ctggtcctat ccgtatcgga caaggggttg aatttgacta tgcgactgtg
    34441 cattctgtca aagcaatcca gcaggcagga tacgaagcaa tcatcatgaa cagcaatcct
    34501 gaaacagttt cgactgactt ttctatttcg gataaattat attttgagcc attgaccttc
    34561 gaagatgtga tgaatgtgat cgagctagaa cagccgatag gagtaatcgt tcaatttggt
    34621 ggtcagacag caatcaactt agccgaacct ttgactgctg ctggtgtaaa aatcttaggg
    34681 acaacgatcg aagacttgga tcgtgcggaa aatcgtgatt tattcgagca agcgctgaaa
    34741 gagttggata ttcctcaacc tttaggggat actgctacaa gtaaagaaga agccgtggtt
    34801 atcgcttcga gaattggtta tccagttctt gttcgtccaa gctatgtatt aggcggacgt
    34861 gcgatggaaa tcgttgaaaa ccagcgagat ttagaagatt acatggaaca tgctgtgaaa
    34921 gcatctccag aacatccagt actagtggac cgttacttgc ttggtggtga atgcgaagtg
    34981 gatgcgattt gtgacggtga aacagtactc atcccaggga tcatggagca tattgagcgt
    35041 gctggtgtcc attcagggga ttcaatggct gtgtatccac ctcaaacatt atctgaatcg
    35101 atcaagaaaa cgatcgaaga ttacacgatc cgcttagcat taggtttgaa ttgtgtcggg
    35161 atgatgaata tccagtttgt catccatgaa aatgaggttt atgtgatcga agtcaatcct
    35221 cgagcaagtc ggactgttcc attcttaagt aaggtgacag gaattccaat ggcacaaatt
    35281 gcgacgaaag ctattttggg agagaaacta aagacacttg gttatgaaaa cggactttat
    35341 ccagaaacaa aagaagttca tgttaaagca cctgtctttt cttttacgaa attgcagaaa
    35401 gttgatactt acttagggcc tgaaatgaaa tcgactggag aagtaatggg atctgacaga
    35461 caattagaca aagctttgta caaggcattc gaagcttctg gattacactt gcctgaatat
    35521 ggagccgtat tatttacgat tgcggacgaa acaaaagaag aagcactaga attagctaaa
    35581 cgttttacag aaattggcta tagtctgata ggaacgaaac atacagctgc ttatttcgaa
    35641 aaaaatggtc tggtcgttga aacagtagcg aaaatttctg aggaagcagc agaaaaaaat
    35701 gtagtcgact taataagaga tggtaaaaca caagtggttg tcaatacgat tgataaagat
    35761 cggggaaatg cttctaaaga cggtttcatc atccgaagag aagcagttga acatggtatt
    35821 ccattgttta cttctttaga cacagcagat gcgatcgttc gagtgatgga atcccgtgct
    35881 ttttcaacta accctattta acggcgagca catgttaaga caaaaagacg aaaaaccgtt
    35941 gcaggagggg acagatgaaa caagaaatga tggaaatcgt tcagcaaaaa cagctggcac
    36001 caaagatttt tgaaatgaca ttgaaaggtg atctagtaaa agaaatgcag caaccaggac
    36061 agttcttgca tattctcgtg ccgcgtgcag acttgctatt acgccgaccg attagcttga
    36121 atcatatcga ccacgaaaca aatacttgcc gtatcattta tcgggtggaa ggtgagggga
    36181 ctagagtctt ctctactctt tctgccggag accatttaga tgtcatgggt cctttaggca
    36241 atggttttga tttgtccaat cttcatacag gagacgaagt atttataatc ggtggaggaa
    36301 tcggtgtccc tcctctatat gagttatcaa aacagttagt cgaaaaagga attaagccaa
    36361 cgcatttttt aggttttgcc acacatgaag tcgtttatta tgaaaaagaa tttaatcgtc
    36421 taggagaaac aagaatcgct actgacgatg gaacatacgg tatgcacgga aatgtcggta
    36481 atttattgtt aggaacgaag acggaaccta cagctgtttt tgcatgtggt tcaaacgggt
    36541 tattgaagac agtggaacag ctgttttctt cgcatcaaaa tgtccagtta tcattagaat
    36601 cccgcatggc atgtgggata ggggcttgct atgcttgcgt ttgtcatacg ccggaagatg
    36661 aaacaggaac aaaaagtgtg aaagtttgtg acgaaggacc gatttttaaa ataggagagg
    36721 taattatatg aatgagaatc ctttagcggt caaacttcct ggcctagaat taaaaaatcc
    36781 tatcatgccg gcaagcggct gtttcggttt tggcaaagaa tacggaaaat actatgattt
    36841 gaatcagctt ggatcgatca tggtcaaggc aacgactcca aatgcccgct ttggcaatcc
    36901 cacgccaaga gtggcggaaa caccatctgg catgttgaat gcaattggtt tgcaaaatcc
    36961 tggtctagac gttgttatgc ataaacttct tccagaatta gaggagtaca atgagttacc
    37021 catcatcgca aatgttgctg gtgcttgtga agaagactat gtagaagttt gtagcaaaat
    37081 tggcgaagca ccaaatgtga aagcaatcga gctgaacatt tcttgcccaa atgtcaaaca
    37141 tggcggaatt gcttttggaa cagattctga tgttgctttt caattaacac aagcagtaaa
    37201 gaaagtttct tctgttcctg tctatgtcaa attgtcccca aatgtaacag acatcgttcc
    37261 aatcgctcag gcaatcgaag ctggaggggc agatgggttt acgatgatca atactcttct
    37321 tggtatgcgg attgacctta agacaagaaa accgatttta gccaatcaaa caggtggact
    37381 ctccggacca gcaatcaagc ctgtcgctat tcgtttgatt caccaagtag caagtatatc
    37441 cagccttcca atcattggga tgggcggcgt gcagacagtg gatgatgttc ttgagatgtt
    37501 tatggctgga gcaagtgctg tagctatagg gaccgcgaat ttcaccgatc cgtatatctg
    37561 tcctaaacta attaaagagc tccctgtgcg aatgagcgaa ctaggcatcg aatcgctgga
    37621 aagattaatc aaagaagtga gagaggaacg tgaacgatga atcaactaga ccaacgacca
    37681 attattgcat tagatttttc aactaggcaa gaggtagaag actttcttcg atttttcccg
    37741 aaagaagaaa aattgtttgt gaaaatcgga atggaattgt tctaccaaga aggacctgaa
    37801 atcgtacgtt atctaaaaga ggctggtcat aatgtatttt tagatctgaa acttcacgat
    37861 attccgaata cggtcgaaaa agcaatgcgg ggattggcaa agcttggtgt tgatctcacc
    37921 tgtgttcatg ccgctggcgg tattcgtatg atggaggcgg caatgcgagg attagaagag
    37981 gggactccag aaggtggaaa acgtccgcta cttctagcta tcactcagct tacttctaca
    38041 agtgaagaag aaatgcatgc cgatcaacta atcgaagttt ctttagaaaa aagtgtgatc
    38101 cattatgcta gctgtgccaa aaaagcagga ttagatggtg tggtttcttc tgcttgggaa
    38161 gtagaagcga tcaaagaaat ggcaggagac gaatttgttt gcttaactcc gggtataaga
    38221 ccagaaggaa cagtcgcagg agatcagaca agagtagtca caccttctca ggcaaaaaag
    38281 atcggctcaa cttttatcgt tataggtcga ccaatcactc agtcaactaa tccttataaa
    38341 gcatatcaaa cgattcaaac agaatggagc taacctaaaa tgaatgttga acaaagtatc
    38401 gcaaaagact tattagaaat tgaagcagta tttttaaatc caagtaatcc ttttacatgg
    38461 gcaagtggaa tcaaaagccc aatttactgt gataaccgaa tcacgatgag ctatccaaaa
    38521 gtccgaaaag agatcgcaaa aggattagca agcaaaatca aagaagctta ccctgaagta
    38581 caagtgattg ctggaactgc aacagcagga atccctcatg ccgcatgggt agcggaaata
    38641 ctggatttgc caatggtcta tattcgaagt aaggcgaaag atcatgggaa aggtaaccaa
    38701 atcgaaggac gtatatttga aggtcagaag atggttgtga tcgaggattt gatttctaca
    38761 ggcggaagtg tcttggaagc agctgaagca gcaaaaagag aaggtgcgga tatccttggt
    38821 gttgcggcta tctttacgta tgagttgcca aaaggaaaag caaatttcga aaaagccgaa
    38881 atccctctta tgacgttgac caattattct gtattgattg aagcagcttt agaagatcgt
    38941 tatatcgatg agcaagaatt aactctgcta aaagaatgga aaaaagatcc tgaaaattgg
    39001 cagattgaag cgcgcttgat gaagatagag aaatccgatt tgtgtctcca gaatcatcaa
    39061 ttggaatagc aaaaatccaa aaactgattg cgagaagcat cttttcatca ggtatgaaaa
    39121 gaaacttcta tcgtgatcag tttttgattt ttttaaaatt catttttcta agactggtta
    39181 gcagaaagaa gagaataatc tcttctcttt tttctattat atatagattt tctctatttt
    39241 ttggtaaggg agctgtttca aaaatggtaa agagcggaaa tgttgcgaat atcagttatt
    39301 taatgatatg atattcggaa aaagaaatct caaagggagg taacttttat ggggttgaat
    39361 gaatttattt taggattgaa caatttagag acgatcacaa gggcacctgg attttttaaa
    39421 tatacagagc atacagttgc agcacattcg tatcgtgtag catctattgc gcaagttcta
    39481 ggagacattg aagaagcgaa tggacaaccg atcgattgga aactgctcta tgaaaaatca
    39541 ttgaatcatg actacacaga gcgttttatt ggtgatatca aaacgccggt gaaatacgca
    39601 agcaaagagt taagagggat gctgcaaaca gttgaagaaa agatgacgga cgaattcatc
    39661 aaccaagaga ttccagctga gttccaagaa gtttatcgcc gtcggttgtt cgaaggaaaa
    39721 gatgaaaccg tagaaggaga actattagcc atttcagata aggttgacct tctttatgaa
    39781 tcttttgatg aaatcatcaa aaataacccg gaaaaagttt ataaggagat gttcattgag
    39841 gcagtgacta cgattaaaag ctttgaacat cggccttctg ttcagtattt ctttaaagag
    39901 atatttccgg aactattgaa ccaggaattt tatggaaaag aggcgttttt agaaaccgtc
    39961 tcaacgattg taaagaaaga agaaaaataa aaatgatatg agatgcaggg ggaagtatag
    40021 ttgaaaaaga atatggatgt cgaatggggc tattctggag acaaaggtcc ggaaaattgg
    40081 gcgagtttat gtgattggtt tgcgagaggt gcggaatttc cattgcagtc gcctattcat
    40141 ttgaccaagg gggaagaaac caaaatagaa ggagacacgc tttcctttca ttatcaaaag
    40201 caacggttta ctgacaagga attcaaaaac acgattcatt tagtaccttt tgaccaactc
    40261 agttatgtag agttcaagaa agaacgctat tatttgacag atattcattt ccacttgcct
    40321 tctgaacata tcatcaacgg aaaacaggaa gatattgaat ttcattttgt tcatatgaac
    40381 agcaaaaaag agaatctagt tgtgggtgtc atgtaccagc taatggtaca ggaaggttgg
    40441 ctgtataatc aagaaaatgg ggaaagttgg aacttagaaa aacatgagca ttgggtaaat
    40501 ccggatgtgt tctttccgga gcaaaaaagc catttccatt atattggctc attgacaact
    40561 ccgcctacca gcgggccaat ccaatggttt gtaatggatc atgtaggaaa actgaaccaa
    40621 gagtttcttc tccccttcga tggtcagtat gctcgaccga acaaccgacc aatccagccc
    40681 ttgaatggaa gagaaattta ttatttcgag gagtttgagc aatgaatatt cagcagatga
    40741 aatacgtggt cgctgtagca aacaatggta gtttcagaga agcggcaaag aaaatgtttg
    40801 tctcccagcc aagtctttca gctggtatca aagaattaga aaccgagtta gggatcacgc
    40861 tgtttactcg aacgaatcga ggggcatatt taacagaaga agggcaagac ttcttaaatc
    40921 gtgccgagaa aatattggtc cagcttgata cattagaaac tcactatcta acgagtgaaa
    40981 agagagacaa tttttcgatc gctgcccagc attatgattt cttaggtcct ttgaccgcac
    41041 agctactcga ggaattcaaa gacgagatca aacaatttcg gatcatggag acgacaacag
    41101 cgagagtgat cgaagaagtc aaagagcagc acagtgagat cggcattatt tatatgaatg
    41161 agcataatca agctggtatc agaagatatt tacaacaagg agagctaagt ggccgcagca
    41221 ttggaaaatt caaaacgcat atttttttaa ggcaaggaca tccattagct gaacacgcag
    41281 agatcaccaa agaagaattg ttatcttatc ctcaagttcg ttttacacag gatggaaata
    41341 atttcccgta tttttatgaa gaccttatcg agatccctga tcaagaaaca gtgatttata
    41401 cgagtgatcg aggtactttg atgaatatcg ttcttgaaac tgatgcttac gcttcaggat
    41461 ctggtattgt gattggaggg attcgcgagc acttgcggtt aatccctttg gcagaaggcg
    41521 aactaaatga attttatatc atttattcgg cgaaacgtcc gctcagtgaa atcgctcaaa
    41581 gatttatcaa agagcttact cggttactgg accagcagag ctaagaaaag cagatgagaa
    41641 cgctgtttat tgaacaccgt tgatcaaaaa agaggggttg tgacagaatt ttgtcacaac
    41701 tcctcttttt gtagcaaaca atttataact aattgctgtt gtgggaaagt acgcaaatat
    41761 gattctagaa acactcattg attacctgtt ttgtttcata gctgtaattt tttcttcttc
    41821 tggtgtcacg ataatcgtct tttggttttc ataaataaca taaccaggct tggcgccatt
    41881 tggttttcgg atatgcttta cctgtacaag atcaaccggc acttgggcag agtatcggta
    41941 ttttgaaaaa taggcagcta actcggcagc ttctgtaatt gtttcatctg agggcttgtc
    42001 acttttaatg atgacgtgcg aaccgggtat attttttgca tgaagccaat agtcggtttt
    42061 tttagctgtc ttcattgtca gctggtcatt ttgaagattg tttttaccaa caagaatgag
    42121 cgttccgtca gaagaaaagt attgatcagg ctgagatggt ttcttacgtt tttgtttttt
    42181 taagttcttc tttttcaggt atccttctga agtcaattct tctttaattg cttcgatatc
    42241 cattgggccg gcaatttcca gttgagataa gacagattca agatattgga tttcatcttt
    42301 ggcttcttgg atttgttcgc caatcagctt aacagcgttc ttcagctttt ggtatcgatg
    42361 gaaatatttc tgtgcatttt gatttggtgt caatgccgga tctagtgcta ttttgattgg
    42421 ccgatcttct tcataataat taggtaaaac aacttctttt gcgccgcgtg gtacttgtgt
    42481 cagaaaagta gtaagtagtt cgccatttct acggtaattc tcagcgtttt cggattcttt
    42541 gagtgtttgt tcacgttttt tcaatttatt tttatttcgt tttagttcat tttcgatttt
    42601 tcgaatcagt tctccgcctt gttgttttgc ccgatctttc tctgcttttt cgtgataata
    42661 gctatctaat aattgactga gtgttggata agctgttaca gcagatgcct gttcggacaa
    42721 tacctgataa gcaatcggtg tgaaaaaatc tttattttcc gtttcgtaaa aagtaggaat
    42781 aggctgatgg ctgatggctg agaaaaattc gtcccataca accatctttt cattaggacg
    42841 ttccagtaat cttttggtca gctcttgagc gatatcgaaa ccgattccct gaaactgacg
    42901 ttggattgct tttggatcta gttcagtttg ggaaagccgc tgaaagattt tttctttctc
    42961 ggagctaaat gggttcaaga cctcttgttt tggcggagca acatattcta caccaggaag
    43021 caaagaacga taagtgtttt gagagcttcc aatgtgtttg atcgcatcca gtatttttcc
    43081 agtttctcga ttaactaaga cgatcgtact atgacgtccc attaattcaa cgatcaatat
    43141 gatattttgt agatcaccca actcgtttcg tttagcaaag tggaaatgga tcactcgatc
    43201 attttcgatt tgttcgatgt cttctaaaat agcgccatcc aaatattttc gaagcatcat
    43261 cacgaagtta ggcggattat caggattctg ataatcgatt tgggtaatct gtacacgagc
    43321 ataacttggg tgcgcagata ataataatcg atggtttttg ccgcgtgaac gaatcaccag
    43381 cacgatttca ttttcataag gctgatggat tttcgagatc cggccagata gtaacgtttc
    43441 acgcaattcg ttgaccatcg cgtgggtaaa tacaccatca aaggacatgg ttcattcctc
    43501 atttcttttc ataagtatat tcattataac gaaaaaaaga agaaaaacca atttggccga
    43561 atagtaaagc aaaagtctag aaaaatcttg gactaattac ataaaaaaca gtgtttctat
    43621 cgttgaaatc caaattttta ggt
//
LOCUS       DAZUKZ010000014        40944 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000014 DAZUKZ010000000
VERSION     DAZUKZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.014"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(233..1015)
                     /locus_tag="ACG8EF_000709"
     CDS             complement(233..1015)
                     /locus_tag="ACG8EF_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946058.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1080..1916)
                     /locus_tag="ACG8EF_000710"
     CDS             complement(1080..1916)
                     /locus_tag="ACG8EF_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4946059.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1934..3343)
                     /locus_tag="ACG8EF_000711"
     CDS             complement(1934..3343)
                     /locus_tag="ACG8EF_000711"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4946060.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3357..5180)
                     /locus_tag="ACG8EF_000712"
     CDS             complement(3357..5180)
                     /locus_tag="ACG8EF_000712"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4946061.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6023..6349)
                     /locus_tag="ACG8EF_000713"
     CDS             complement(6023..6349)
                     /locus_tag="ACG8EF_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946062.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6395..6757)
                     /locus_tag="ACG8EF_000714"
     CDS             complement(6395..6757)
                     /locus_tag="ACG8EF_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFS4946063.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6794..7009)
                     /locus_tag="ACG8EF_000715"
     CDS             complement(6794..7009)
                     /locus_tag="ACG8EF_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4946064.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7136..7471
                     /locus_tag="ACG8EF_000716"
     CDS             7136..7471
                     /locus_tag="ACG8EF_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4946065.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7547..9544)
                     /locus_tag="ACG8EF_000717"
     CDS             complement(7547..9544)
                     /locus_tag="ACG8EF_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946066.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9642..9917)
                     /locus_tag="ACG8EF_000718"
     CDS             complement(9642..9917)
                     /locus_tag="ACG8EF_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4946067.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10149..10883
                     /locus_tag="ACG8EF_000719"
     CDS             10149..10883
                     /locus_tag="ACG8EF_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4946068.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            10922..12064
                     /locus_tag="ACG8EF_000720"
     CDS             10922..12064
                     /locus_tag="ACG8EF_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4946069.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12159..13550)
                     /gene="sufB"
                     /locus_tag="ACG8EF_000721"
     CDS             complement(12159..13550)
                     /gene="sufB"
                     /locus_tag="ACG8EF_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFS4946070.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13555..14025)
                     /gene="sufU"
                     /locus_tag="ACG8EF_000722"
     CDS             complement(13555..14025)
                     /gene="sufU"
                     /locus_tag="ACG8EF_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFS4946071.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14012..15247)
                     /locus_tag="ACG8EF_000723"
     CDS             complement(14012..15247)
                     /locus_tag="ACG8EF_000723"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFS4946072.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15244..16530)
                     /gene="sufD"
                     /locus_tag="ACG8EF_000724"
     CDS             complement(15244..16530)
                     /gene="sufD"
                     /locus_tag="ACG8EF_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFS4946073.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16543..17316)
                     /gene="sufC"
                     /locus_tag="ACG8EF_000725"
     CDS             complement(16543..17316)
                     /gene="sufC"
                     /locus_tag="ACG8EF_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFS4946074.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17513..18361)
                     /locus_tag="ACG8EF_000726"
     CDS             complement(17513..18361)
                     /locus_tag="ACG8EF_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4946075.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18385..19071)
                     /locus_tag="ACG8EF_000727"
     CDS             complement(18385..19071)
                     /locus_tag="ACG8EF_000727"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS4946076.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19064..20098)
                     /locus_tag="ACG8EF_000728"
     CDS             complement(19064..20098)
                     /locus_tag="ACG8EF_000728"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS4946077.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20161..20417)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20493..20831)
                     /locus_tag="ACG8EF_000729"
     CDS             complement(20493..20831)
                     /locus_tag="ACG8EF_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFS4946078.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(20833..21189)
                     /locus_tag="ACG8EF_000730"
     CDS             complement(20833..21189)
                     /locus_tag="ACG8EF_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFS4946079.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21195..22382)
                     /locus_tag="ACG8EF_000731"
     CDS             complement(21195..22382)
                     /locus_tag="ACG8EF_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS4946080.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22573..23418)
                     /locus_tag="ACG8EF_000732"
     CDS             complement(22573..23418)
                     /locus_tag="ACG8EF_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4946081.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23652..24617
                     /locus_tag="ACG8EF_000733"
     CDS             23652..24617
                     /locus_tag="ACG8EF_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFS4946082.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            24994..26037
                     /locus_tag="ACG8EF_000734"
     CDS             24994..26037
                     /locus_tag="ACG8EF_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFS4946083.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26099..26563)
                     /locus_tag="ACG8EF_000735"
     CDS             complement(26099..26563)
                     /locus_tag="ACG8EF_000735"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFS4946084.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            26809..27756
                     /locus_tag="ACG8EF_000736"
     CDS             26809..27756
                     /locus_tag="ACG8EF_000736"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4946085.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(27967..30039)
                     /locus_tag="ACG8EF_000737"
     CDS             complement(27967..30039)
                     /locus_tag="ACG8EF_000737"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4946086.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(29972..30349)
                     /locus_tag="ACG8EF_000738"
     CDS             complement(29972..30349)
                     /locus_tag="ACG8EF_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFS4946087.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30478..31038)
                     /locus_tag="ACG8EF_000739"
     CDS             complement(30478..31038)
                     /locus_tag="ACG8EF_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4946088.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31188..32480
                     /locus_tag="ACG8EF_000740"
     CDS             31188..32480
                     /locus_tag="ACG8EF_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS4946089.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(32540..33658)
                     /locus_tag="ACG8EF_000741"
     CDS             complement(32540..33658)
                     /locus_tag="ACG8EF_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFS4946090.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(33672..35093)
                     /gene="arcD"
                     /locus_tag="ACG8EF_000742"
     CDS             complement(33672..35093)
                     /gene="arcD"
                     /locus_tag="ACG8EF_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFS4946091.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35339..36460)
                     /gene="mnmA"
                     /locus_tag="ACG8EF_000743"
     CDS             complement(35339..36460)
                     /gene="mnmA"
                     /locus_tag="ACG8EF_000743"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFS4946092.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            36864..37463
                     /locus_tag="ACG8EF_000744"
     CDS             36864..37463
                     /locus_tag="ACG8EF_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4946093.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(37601..38887)
                     /gene="efmA"
                     /locus_tag="ACG8EF_000745"
     CDS             complement(37601..38887)
                     /gene="efmA"
                     /locus_tag="ACG8EF_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFS4946094.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(39158..39502)
                     /locus_tag="ACG8EF_000746"
     CDS             complement(39158..39502)
                     /locus_tag="ACG8EF_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFS4946095.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(39639..40784)
                     /locus_tag="ACG8EF_000747"
     CDS             complement(39639..40784)
                     /locus_tag="ACG8EF_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS4946096.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT        11759 a         8621 c         7169 g        13395 t
ORIGIN      
        1 aaatttctta aaaatttttt cactaaaatc actacctgaa acagtctaaa acacttatat
       61 atcaacgttt aatgaaacta tctccgtttc atttcagata caaataaata tagtcgggct
      121 ccaaatccga aaaaaaatac ccccagtagc ttatcctaag ctactggaga tgtataagta
      181 cggtcatata acaacggggt agggtaaata aatgaaaaag atggaagaat cattattcaa
      241 taactttttt aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat accacagaat
      301 aatctttttt cccattttca agtaaccgaa gtggaatgtc tttaccaact tgctctgtca
      361 ctgcattact gatacctgtt agattagcta ataaatctga taaagattga gcatctgtag
      421 aaatctctgt atcagtattt atcgtgattc ctttatcttc atttacaagc ttccaagtag
      481 gtggtatagc tgtgtatgtt tcgatgaatg aatcaattct ttcatctatc gtcttcccag
      541 tatctacttc tgtctcatta ctcttagatg aatcgattct ttcatccatc gtctgctccg
      601 taactgcttc tgtctcatta ttctcaccgg aaactctctt cacacccaaa cttatatatt
      661 gtccatcttt agtgtgcata gttaaaattc ccacgctttt actaataaat tcactataac
      721 cagttctttc aatagagact tcaatgtatt tcacactatc atactcaaaa gaaaaataga
      781 catgtaaaag cattttgttg tttacatcta cataataatc ctttgactct acttgagacc
      841 ttcctatacg gaatacagct ttactgtctg atatctctat accagcatcc ttattattaa
      901 tctgccaatc tccaagtagc tcttctggca agctagtagt ttcttgtgaa cactttacca
      961 cttcactggt agaggtcgaa acattcacct ccgcactgtt agaagaattt tccaaggaat
     1021 caggagcctt agcacaacca acaagtcttt atatattgtg cagccgatat tttcttcatt
     1081 cagtacaccc ttgaaatatg cagtgttaga taaagcagtt cgtcctgatt tgctgaggca
     1141 cccatctctt cttgaacgta ctccttgatt ttcaaggccg cccgatactc tttcggaaaa
     1201 cgctgcttca ctgaagccag caaaaaatca tccttctccg gcaactcttc cttcttaatc
     1261 attcgggcaa taaaaaactg taaatgcgtc acaaatctta agtaaagcat cgaatcttta
     1321 tcaacctttt tttcgggaaa cagatatcct agcatagcca agatatcctt tagtgctctt
     1381 accgagagca tggtttcttc catatttgct tgttcacttt gggcatttac aatgtgaaac
     1441 gcaatgtttc cagcctcctc ttctggcaac tgaacattca tttttttgtt gatcaattgg
     1501 atagcatagc agccgatctc aaattctttt gggtaatact tttgaatttc aatcagcagg
     1561 cgattttgaa aaacaatgcc tttttgactg cgttcaatgg catgcttcaa gtgatcaacc
     1621 aaggtaaaaa atagactttt attcagctta aagtcaaact tggcgttggc ataatcgata
     1681 attcgcttga cgcaaataat tagatcatcc ggagcagcct caaacagttt aatgtaatcc
     1741 ttttttagag catcagaaag tgaatctaat ttaaatactt tttggatttt gtcagccgga
     1801 accaaatcgc ctttctttag ttgaaatcca atccctttcc ccatcaaaat tacttctttg
     1861 ccttgcttat ctaagcaaag aattaaactg ttgtttaacg ttttaattac tctcattaga
     1921 ttcaccgttc tctctaagct aagccatttc ttgcgattac tttttgatac caaaagaagc
     1981 tttctttttt tattcgtttt aattctttaa gattctcatc ggttcggtca acatatataa
     2041 aaccataacg cttattgaag ccttgatggg tactaacaac atcaatcgct gaccaaggta
     2101 aataaccaaa aacatctaca ccatctgtta aagctgcttg aatagccaga atatgtttgg
     2161 ctagataatc aattcgatcc tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg
     2221 ccccatagcc attttcagta attaaaatag gtaaatcata gcgatcgtac aatttcctca
     2281 atgttaatcg tagtccagta gggtcaacta cccagccata tggcgtttta tctacataag
     2341 gattttcagc tgcccggtag acaccttctt ctcccaacat gatctgttga tcccccgctc
     2401 tagcggaaac atctgatccg tcatttttgc tggcagcgat ggtggcagtt gaataataat
     2461 taatcgcaat aaaatcgggt ttaccggagg ataaaattgc ctgatcctct gcctcaattt
     2521 taggatataa tccacgatca ttcaaatagt tttcaaacaa ataattgtat tttccccgaa
     2581 ctgctacatc taagaaagac cagcctctca atgtttccca attatgggct gcaattgcat
     2641 cttctggact ggaggtagct tgatacatag ccgttaaatt gattgctgga ccgatttttc
     2701 ctttaagatt caactgatga tacaagtgaa ttactctagc ttgagctaat aaaacatggt
     2761 gattaatttg aaagagttct ttttttgaag gcagctctcc acttggtggc aaaccaatcg
     2821 ctccaggatg aaggatcatc gtgttttgct cattaattgt cagccaatag ttcaccttgt
     2881 caccatactc agtaagcaat acctgtacat agtccacaaa ataatcgata gttgctcgat
     2941 ttaaccaacc gccctgctcc tctagaaaat aaggtaaatc gaaatggtac atggtgacaa
     3001 tcggttcgat tccctgattc aggcattcat caattacttg atgataaaaa gaaattccct
     3061 tttgattggt tttattattt tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa
     3121 aagcttttaa gcctaattct ttaaacagct gtatatcttc tttatagtga tggtaaaaat
     3181 ccgaggcaac ttcaaaatta gctacccctt ctgtatgctg atacttgtca ataatagacg
     3241 gttgtttccc atcctcctga taggcaccct ctgcctggta ggcactaatg gaggcacccc
     3301 ataaaaactt ctctggaaat gctgaaagct gtttgtattg cacaaaaatc ctctccttaa
     3361 ctaatttgca tcaatggttc gtgttccttt aagcttccgc ttgcttcact aattcctgcg
     3421 taattttcac tgttggtcac taccacaatc actgtatcat cgtagccagc gtttctaatg
     3481 gcagcgaaat cggcgttgat taataattgt cctgctgaca cctgatctcc tgcttttaca
     3541 aatacttcaa agggttcccc ttttaaattc acggtgtcga tccccacatg gatcaataat
     3601 tcaatacctt ctttagaacg tagaccaatc gcatgtttag attctaaaat agtaatgact
     3661 tctccctcaa caggtgaata aatgctgttt tcttctggat gaatggctac gccctttccc
     3721 aatagtccct tagaaaagac agcatcgttc acttcagaaa taggacacac ggttcctttt
     3781 accggatttt caacaatgac tgcttcggct attgccgttt gctctgattc atcagaagta
     3841 tcgtcaaatc caattagata agcaatgata aaggttgcaa caaaggcgat tacacacgta
     3901 ataattgcgt gaacaatatt cattggattt tctccaataa aggctggtaa ggctgctaag
     3961 ccaggggaaa caaaggcata tctgactaaa ccgctcaagc cagcatacaa accagcaatt
     4021 gcaccaccaa tcatggaagc aactaatggt tttttcagct tcaaggttac tccatagagt
     4081 gaaggctcag taattcccat taaagcagtg atcccagtgg acacagctag ctgcttcaac
     4141 tctttgtttt tagttttcgc ggctacagca aaggtagcac ctgattgagc aatattagaa
     4201 gccagcattc ctggcccatt gatcatttca gaacccgtac tgctcaattg catagtagcg
     4261 attggcgtca ttgcccaagc tgtacccgta accaccaaaa atggctgcaa accacccatc
     4321 aaaaatggaa ttaaccaagc tgctttgccg ttaatccatt cagcccccat tgctataaga
     4381 tcgttcagca acgttccaaa cggtccaatg acaaccaacg ctaaaggccc cataattaaa
     4441 aataccagca tcggcttagt aaagaattta ataaacgaag gcgaaattcg atccgcaaag
     4501 ggctctacat aggacatgat ccaaacagta aagataatcg gtaaaactga tcccgcataa
     4561 tctgcagcta ataccggaat tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt
     4621 gaaatatttg gatgaagcaa aatgccagca acagtaatcg ccaaaattgg gttgacatca
     4681 aactttatgg ctgccccata ggctaatagt actggcataa aataaaaggc tgcatcagca
     4741 atagtattca aaatgatgta agtttgacta ctgtctgata gtacaccagt caaagtcaag
     4801 acggccaaaa tggcttttac catcccggca cctgttatag ctggaatcat cggtgtaaag
     4861 gtggtagaaa tgacactaat caatcttgaa ataatacttt cttgattgtc tgaagagggg
     4921 ccctttttaa ttgaagttcc cacttccttt tgaatttcct tgaaggctgt ttggacttca
     4981 ttgccaatca ccacttggta ttgtccaccg ctatccacca cactaattac accaagtgtt
     5041 ttttctaagg ctttcgtatc ggctttcctt ttatctttga gttcaaaacg cagtctggtg
     5101 gcacaatggg ttaagctgac cacattttct tttcccccaa cctgttgcaa tacatttctt
     5161 cctacttctc tgtaatccat tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa
     5221 ccacgcccca atcaacattg aagtgttagt ttgtcttggt cttgcctgca taatcagtaa
     5281 catgccaata acgttatttt gctgtaatcc aataatagca cattatgata gcgcaatcaa
     5341 cagggaattt aattttttat cttttcgttt gaccgtatag ggtaatataa cagttagatc
     5401 acatcaacta gggtatatct gcgtatactt actgctaaaa taagtatatc taatattaac
     5461 tataccagta aaaccattcc tacataacca gcgggaaatt cccaaaatcg tgaaagcatc
     5521 aaaaatgcat ataacggatc acacaacttc aacaattctg ctcttgggaa aaattgatga
     5581 atttggtgag taaaggatgg aataaacagc attccaacaa tataggcaac aatagtgata
     5641 cttgtttttt tggtaaaaat cagtaagcaa tagccaattt caatcattcc gagatataaa
     5701 ggtatcgata gaatgatttg ggctagtatt cttaaaaatt aactagcaaa tgtttaaact
     5761 gatttatttc taatctacat gtagtaagta tttcaattcc accaattatc aataaaaata
     5821 ccaaacatat ccataataat tttttctttt tcatttcttt tatattgtta taacagtgaa
     5881 taacataaaa attcccgaca atcattgtaa tatttttttg tcacctgtat tagtatttaa
     5941 tttataggaa actataaaaa ctccctagtg aatttacacc tttgggagtt cctgtttgga
     6001 cggtatgcat aatacattta aatcaattta acattaacac ctcagcatat ggtatctcca
     6061 catatatacg gtctttcttt ggatagtact caatatttat gttgatagca cttccattgt
     6121 ctaatatagc tttagcccgg tatgtgttat ctccattttc atgagtaatc gtcacatcat
     6181 cacatgatcg atttaagttg tactgttctt gtaaaatctg tgttaccaat ggttttgatg
     6241 acttctctaa tccagatgta cctgtcattt ttgaaaaaat aatacctata atagccaaga
     6301 caacaatacc tataatgaca aacttcttgt tcatatttac tttattcaac tttttatctc
     6361 ctatctccaa aataatttgc tcttatccct cttatcagta cattgacatc cctaaaagta
     6421 caccaaagat tgctccagct acccctgcta tcatcaaaat cgtacccatt ttctcatctt
     6481 tttctcgata taagtagccc ataataacag caacagcacc aaatacaaca ggaataaatg
     6541 ctaacgatac taccatagaa atccaaccaa taatcaaata cagcttccct gtctcttgtc
     6601 catgtacgat ttgttccttc atttcattaa ttgtctgctc agtacttttc tttactgtgt
     6661 ctgttccggt tgtctcatct gtttcttgta gtgctccaca aaactggcaa tacttacttt
     6721 catctgttat ttctttgcca cattttctac aatacatgtc ttctcctcct aaaatcattc
     6781 aattgcttat tagttaactg tttactttat taaatacatc agaaaacagc tcattcattg
     6841 gtacacttaa tatctcagaa agcatcttca tttgataggg tttaggaatc gttctccctg
     6901 tttgccacga acttacacat tgctgtgtaa cattcatttt ttttgctaac tccgtttgag
     6961 aaagtccttt ttcttttctg tatcggctga gattacttac ttttttcatg attctcccct
     7021 cctgttttat tatatacaac aatatgatgt atttcaactc ataaaatata aaaaacttca
     7081 aataccatta caaatacaac aacttgttgt actatactga gatgggggtg tattaatgaa
     7141 acagaaacaa tatgcaaagg tcattagaga aaagcgaaaa gctctcgggt ggacacaaaa
     7201 agaattagct gaaaaaatct tctctacaca acaggcagta gcaagatggg aaaattctgt
     7261 aacagagcct aacctcgata gcttaacagc tctttctaga gccctaggaa cccctgtaag
     7321 ccattttcta gacaatgttg tcgtagatta tgaggaagag tttttggctc tctatcgttc
     7381 tcttagcact gaagatgcag ttcgaacaat tgactatatg aagctattaa aaaggcaaga
     7441 aaatgaacgc aatcagctat tgaaagattg aaaaataaat aacccaccga actttaacgg
     7501 tgggtgtatt gagatgaaat aagattaaga gctatggata acctgtctag tttaatacaa
     7561 ttgggtcttt tctcttcctt actctccgtc catcatctct tgttacactt gtaaattgat
     7621 acagaggaac tatcccagct cctgataata tcaattcttt tattgtttca tctatccctg
     7681 cccctttcgc taaatatatt aaaaaactac tacttgcttt cacattgtat agtttcttaa
     7741 tatccttaga agctcgttca agagtgaatc cttgtttaac aaagtagcct ctgtttttta
     7801 ttaaagcatt tttcacgctg ttatccccaa cattacgtag gcgttcacaa acagtgttaa
     7861 taagttcttt ctctgtcatt agatatgtcc cttgccacga taagccctgt aaaacttcat
     7921 gtgcttcatc taaaatcaat tgtttttcct tgctgagttc tgatcgggaa ttttgaggaa
     7981 atactctttt agcctctttt tcgttaaaac atatcttaac ccattcataa gaaaaatatt
     8041 gatatgtaaa atgaattttt tccagaagtg gaaggatatc ttcattcaat ttttgaatca
     8101 cagaagttgc attaaaaacg tctaatcgat agacattgct tctcttatga ttaaagtttc
     8161 ctaacgcttt ctcttccttt aacctattta ggtattcgag catcttcaaa ggaacatctt
     8221 catcatctaa tttttttatc aatcctataa aagtcataag tgatagtaac ttattacatt
     8281 tatctatgtc ataagtttta ccatagtaag aactcatttt tttagctaat tgttcccccg
     8341 acaaaataca agtcacactt tccttactat catcaaaaaa attaacatca gaaagcaaga
     8401 catcatagac acgtcgcatt tcctctagat aattccttat tgtcctgaaa agtttgtctt
     8461 tcattgattc aagagtgtca aaaagtactt ttgattcctc tttcaatttt cgaaaatgtg
     8521 taggatacaa gtctaggttc tctaatagcc aattaaatgt ttctacccga ctgtctttct
     8581 ttaaaattag catcacaact ccaataaaat tttcactgat atcacaatta ctggaatgac
     8641 aataaaaaat ggaatgccca gttttactgg taaaaatact agcagaggga ttatgatcgt
     8701 tgtgaaacag acatcttaag tttttacttg acttaagtaa tttattcata tcaatggtta
     8761 acaatctttc ataaatctca ttaaaagaca tgtcggagga atcaaacagg cagtctttaa
     8821 accactgttt catttcttct ttgtcgtcat tttttatcaa cttaacaaaa tcggtttcat
     8881 tgactagttc atactctctc cctgaatcac tttgacaatt tttagaacat gtcatggtaa
     8941 aatcaatcgg aagctcagta atatctaaca tgttgtcttc attaattatt atcactgcat
     9001 ctgacttata tcctccaaaa aacattctat tcgaagcatt tacattgtca tcacacccgg
     9061 ggatgttctt tttcaagtat tcgtaaacag caaccatttt accatagtca gtaattgctt
     9121 cttttaatag aaagacaatc ttgtattttt ctagattttc agagtaccga atactttttt
     9181 gtatcgcaaa cgcagaatta tgaactaaat tgatagataa aaccttttct acgcttaagt
     9241 atctactatc atctttactc aaatctacta ccttcccctt ctcatcttta acagtgttat
     9301 cgacatccag aataattact ctggtttcca caaaactctc cttgtcattg cctttattct
     9361 ttgtcttgct tggaataaat gattgtgatg aatttagtat ctctacaaac tcatctcttg
     9421 taacttctct atagttatct aaacacctat ctcttacatc agttttttct ttttttgtaa
     9481 ctggcttctc taataatttt tcttgatcta cataaattgg aatattaatc attgatgttt
     9541 ccatttgtgt ttcctcctta taaaattctg ttaaagacta aaacatacag ataataaaaa
     9601 caaactcttt attactatat gttttagtct tatttgttta ctcaaccttt ataccgttcc
     9661 atgaaactat caatatcaga ttttttaaat ctaatgaccg tatcaatttt tataacaggt
     9721 aacccgtatt gagaaacata ttttctaatc gttcctactg atacacctag atattcactt
     9781 gcctgctgat agtccatata ctctagagtg actccagaaa taatgtcttc tagcataggc
     9841 tttagcactg taattatctc tttcacttgt tgttcaactt gtatagaaac aatgctatca
     9901 atgatttgta gagacatatt tccccctcct ttcgtgattg cttgaacaaa ttttctaaga
     9961 attatcttcg tattttttgc tcaaaataaa agtattttgt tattaaccga ttaatcaaac
    10021 aaaaaatgat taccagttca tcgacataca cataatacct cgataaaaaa ggggtgtcaa
    10081 cagctttgta aaatttattt gcaacacccc ttttttgagg tatactaatt ttaggaggtg
    10141 tggtcaatat gataaaacgt ccaccaataa attatttaga acgaaaaaag atactcggaa
    10201 caaaaataaa agctataaga aagagtaaaa aattaacaca accagcattt ggtcttatga
    10261 ttaacaacgg tcaattaatt gacaaaaaaa ctatctacga atgggagaag ggaacctatt
    10321 taccaatacc agagcgatta tcccgaattg ctgatttggg aaatatgagt atagaggagt
    10381 tagtttgtgg caatgtagaa gaatatattt tgggaattat tctttataga gatagcattg
    10441 tactagatgg tataacattt ccagataaaa acctgttcca gcatcttcgt caacagttcc
    10501 caccagtaca ctctaattta gatacatggc tggatagata ttccaaatta gaaccagaga
    10561 tgcaagaatt tattgctaac aagacttgta ataaagttaa aaatgaaaaa attagtcttt
    10621 tcaatatctt aaaaatagaa gaattattca tcaatgctat tgtagaagaa tttgacaata
    10681 acattctttt cctcactagc agtatagaag aactactaga aagaatggta gatgaatggc
    10741 tacctattca attgaaagac atgtcttatc ccgaagaagc tgtgagagaa attactgata
    10801 atataaacaa gctagaacaa acgatttcat caattgggaa aaaatacact aagaaaaaaa
    10861 tgaagggagg tgataccatc tagtggtaag tactccttgt aaactaagag gagatgattt
    10921 aatggcaaaa atatcaaagt acaaaaagaa aaacggtcaa gtggcgtgga tgtataaagg
    10981 acacattgcc accgatccac gaactggtga aaaaataaat actacgagaa gaggttttag
    11041 tagcaaacaa gaagctcaaa aagattatga cgagtacaga catcaaatat tatacggtgt
    11101 taaaaagaaa gcacctgata tgacatttga agacctgtat aatgaatgga taactcatca
    11161 gcgtaccagt gttaaagctt caacaatcgc aatctcagta agatacgcaa ataatcaaat
    11221 acttccagca tttggtaagc ttaaactatc gaatatctct gtcccatatt gtcaaaaagt
    11281 ggtagatgaa tggcacagta aatatgaaag ttacgattat atgagaaaac agactgcaca
    11341 aattttacgc tacggggttg caatgcaata tattgacaat aatcctatgg aaaaaacact
    11401 tcttccacga aaaaaagagt atgagaaaaa tcgtaaattt tactcaaaag aggagcttaa
    11461 taatttactg gatgctttta aagattttgg taacatgaaa caatacgcct tcttcagatt
    11521 attggcatac acaggtatga gaaagagtga agtactcgct ttacagtgga aagacataga
    11581 cacgtttaat aaagaactac atgtcaataa aactcttgct gtagatgaat ttggtaaagt
    11641 tataattcaa tctccgaaaa caagagcttc tagacgagtt atctctttag atactgagac
    11701 tctttctatc ctcaacaact ggaaattaca gcaaaaagaa gagtatttaa aacttggata
    11761 caatactagc tctaaagagc aacatgtttt tacaacagtt aaaaacactc tatacattcc
    11821 aaacactgtt aatgattggt taagatatat tttgaaaaaa tataacttgc ctcgtattac
    11881 acctcatggg tttagacata cacatgctag tttattatta gaagcaggag aatctgtcaa
    11941 ggtagttcaa caacgattag ggcatgaaaa tagtaaagtg acattagaca tctatgcaca
    12001 tattacaaat aatgcaccta agaaaacagg tcaagacttt gctgatatga tggctcatca
    12061 gtaaaagagc tggtcaaaat ctggtcattt ctagtataca ggcaaaacaa aaaagctaga
    12121 acccttatat atcaagggtt ctagctccta taacaggttt acccaacact gccttccatc
    12181 tcataactga tcaatcgatt gagttcgact gcatattcca ttggaagttc tttggtgaat
    12241 ggttcaacaa agcccatgac gatcatttca gttgcttcag attcggtcag gcctcggctc
    12301 atcagatagt agagttgttc ttctgagatt tttgagactt tggcttcgtg ttctaaagag
    12361 acttggctgt tgtggatctc attgaatggg atcgtatcgg atttcgacca ttcgtccatg
    12421 atgatcgtgt cacattcgat atgagagatc gaacccgcac tgtttttggc aaaggttact
    12481 tgtcctcgat agtttacttc tccgccgtct tttgaaattg atttagagac gatagagcta
    12541 gatgtatttg gtgcattgtg gatcattttt gcacccgtat cttggatttg gtttgctcct
    12601 gcaaaggcga tggagagcat ggtgcctcgt gcaccttttc catctaagta aacacttggg
    12661 tatttcatcg ttgtttttgc acctaggttt ccgtcgatcc attcaacggt tgcgccttcg
    12721 tatgcttttg ctcgtttggt tactaagttg tacacattgt ctgaccagtt ttggatcgtt
    12781 gtgtaacggc aatacgcatc ttttcttgta aagatttcta cgatggcggc atgcaggctg
    12841 ttacttgaat aagtaggtgc cgtacaacct tcgacatagt ggatgcttgc tccttcgtca
    12901 acaatgatca gcgtacgttc gaattgcccc gtgttttccg cattgatccg gaagtacgtt
    12961 tgtaatggaa catcgacttt gacgcctttt ggcacgtaaa taaaggtccc acctgaccat
    13021 acggctgaat tcagcgctgc taatttgttg tcggttggcg gtacaagttt tgaaaagtac
    13081 tctttgaata attcagggta ttctttcaaa gcagagtcag tgtcagtaaa gacgattcct
    13141 aacttttcga attcatcttt catattatgg tacacgactt ctgattcata ttgtgcggaa
    13201 gcaccggcta gataagctcg ttcagcttct ggtataccaa tcttttcaaa tgtatctttg
    13261 attttttctg gtacgtcttc ccagtcacga gcgggtttgt cacttggttt ttggtaatat
    13321 ttgatggcat cgaaatcgat gtctgaaagg tcggggcccc atgtttgcat tggcattttg
    13381 ttaaatgttt ctagagattt cagacggaat tcaagcatcc attctggttc gcctttgatt
    13441 cttgacatct cacggatcac ttcttctgtc aagccttctc ctgtactgaa aaccggttcg
    13501 acatcatcgt gaaacccgaa tttatattct tctaattctg gtacgcccat gttctcactc
    13561 ctcttccttg tggtaatgat ggaaagctgc ggtaccatcg ttttccattg cttttcctag
    13621 tgctttccag gcaagtgtcg cacatttgat ccgagcggga aatttagcta caccaccaag
    13681 catagcagca tcgcctaatt ggtcgggatc tttgacttct tttccttgga caagctgtag
    13741 aaagtctaca gttaaatgtt ctgcttcttc tattgatttt ccgatgacgg cgtctgtcat
    13801 catcgaggca cttgccgtac tgatggagca gccactgccg tcaaaggcga tatttttgat
    13861 gattccgtct tttacatcga cttgcaattc gatcacatct ccacatgtgg ggttgttcaa
    13921 ttcgatcttg cttgtcgagt cagtcaagct tccacgatga tgcggatggg aagaatgatc
    13981 caagatcact tgacggtata aattgtctaa tttagatagt cccatgttgg aaaaactcct
    14041 ttgttgccat gattgcttca actaaacggt cggcgtcttg cgttgtgttg tagaaataaa
    14101 agctcgcgcg agcagtcgct gatacttcaa ggtacttcaa tagcggttgc gcacaatgat
    14161 gacctgctct gacggctacc ccttccatgt ctaaagctgt cgcgacatca tggggatgta
    14221 acccatccaa attgaatgca acgactcctg tccgaaaatt cgggtcttgt ggaccataaa
    14281 cagtcaatcc atcaattgct tgtaattttg gcaacacata agcggctagt tcttcttcat
    14341 atcgatggat attgtccata ccgagttttt gcaaataatc gattgcatgg cctaatgcga
    14401 tagcgccggc gatatttggt gtccctgctt cgaatttcca aggtagttct ttccatgtac
    14461 tgtcgtataa ttcgacaaaa tcgatcattt cgccaccaaa ttcaacaggt tccatttctt
    14521 ctaaccattt tcgtttacca tataaaacac caatacctgt tggtccgcac attttgtgtc
    14581 cgctgaaagc aaagaaatca cagtctagtg cttggacatc aatggccatg tgcggggcag
    14641 attgagcacc atctactacg atgaccccgc cgttttcatg gacaagttca gctaattctt
    14701 gaacaggatt gaagacacct aagacattgg atacatgagc aatggagacg attttggttt
    14761 tgtccgtaat ctttttcgct gcatcctcca tatccaggaa gccttcttct gttagttcga
    14821 tatatttcaa gacagcacct gttcgttttg ccaactgctg ccacgggata acattagaat
    14881 gatgttccat gtaagagagc actatctcgt ctcctgcttt gatatagcgg tcgccgaagg
    14941 ttctggctac ccaattcaag cttgtcgtcg taccacgagt aaacagcact tctgctgttt
    15001 cttttgcatg gatgaacgcc cgtacttttt ctcttgcttg ttcataatca tgggtcgcgc
    15061 gttctgctaa cgtatggaca ccgcgatgaa cattggcgtt atcgtgttcg taatagtacg
    15121 ctaatgcctg gagtacttgt tttggctttt gggtcgtcgc tgcattatcc agataaacta
    15181 aaggttcatc attgacgtct tgaaataaga taggaaaatc attgcgtagc tttttagcat
    15241 cgatcatcca ttcaacttcc cttcgattac ttcgactaat tctttccgaa cttgttctac
    15301 agggattgct gttaagacag aaccgaggaa tcctcgaatc actaagcgct ctgcttcttc
    15361 tttatgcaat cctctactca tcagatagta catttcttct ggatcaactc gtcctacact
    15421 ggcagcatgt ccagcagtta cttcgttttc atcgatcaaa agaataggat tcgcatcacc
    15481 acgtgcttta tcagaaagca tcaagacacg actttcttgt tgggcatctg caccttttgc
    15541 ccctttcaag atgtggccga taccattaaa ggtcaatgtt cctttttctc ggatcactcc
    15601 gtgttgcaaa atatgcccga tcgtatgagg ggctttattg gttactcgtg tatcgattcc
    15661 ttggatttgt ctgcccgagc tgattgccac gattttgact tctgcgtgtg caccttctcc
    15721 tgctaaatct gagtcaaagt cagcgatcac atgaccgtca ttcatgacac caattgccca
    15781 gtcgacagaa gcatctcgta aaatgtgccc acggcgattc atataagaag taatattttc
    15841 tcctaattga tctacggctg aatatttgat ttttgaacca gctttagcga tgacttccac
    15901 gatgatatta cctgatgatt ttgctacttg ttctttggtt gtctggaaac gttccagata
    15961 agaaaattca ctatgttcat ctgccacgat caacacatgc ttgaagaagt gctcatcgga
    16021 tgcgccatct tgaatgaata aggactcgag tggttcatcg atcacgacat ttttaggcac
    16081 atacaggaaa ataccgctgt tcataaaagc tacatggagt gcagtcaatt ggtcttcgtt
    16141 catgtttact gcttttttca tataatattc ttggactagt tctggatatt cgatcattgc
    16201 agtgaacaag tcggtaaaaa tgactccttt ttctgctaat tccacaggca gttgttcaaa
    16261 aatcgtccat gaaccttgtt ggacaagcac aggattgtct ttcattgcat caaatgccgg
    16321 aatcgtgcct gtttcactga tggtttgtgt ttctttcaca tcaaataacg accagcgatg
    16381 gaatttcacc cgatcgatat gcggcaatgg cagttcatct gctttcgcta gtgcgtcttg
    16441 acggaaggtt ctcatccaat caggttcgcc tttacttaaa gaaaaatcat ttaccgcgtc
    16501 aagataatcg agccagtttt tttctttcat tcttgctccc ccttacgctt cttcttcttt
    16561 atagtcgatt cctaattctt tactgatacc agcgtagcct tctgcttcca gacgtttagc
    16621 taggtcagca ttccctgtca tgactactcg gccttccatc atgatgtgaa cgacatcagg
    16681 tgtgatatag ttcaataatc gttgatagtg ggtgatgatc aatgcaccga aattgtctcc
    16741 gcgcatttca tttactcctt tagcaacgac tttcaaagca tcgatatcca gacctgaatc
    16801 gatttcgtct aaaattgcaa atgtaggttc taacatcaac aattgcagga tctcattccg
    16861 tttcttttct ccgccagaaa aaccttcgtt cagataacgt tcagccattt cttctggcat
    16921 atttagtaaa gccatttttt catctaactt tttcaaaaat gccataacag aaattttgtc
    16981 gttttcatca cgtttcgcat tgatcgctgc acgcatgaat tccgcattcg tgatacctgg
    17041 tatctcactt ggatattgca tagctaaaaa taagccaagt cgcgcacgtt catctacttc
    17101 caagtctaaa acatttttcc cgtcaaacaa aatttcgcct tctgttactt catagtttgg
    17161 attgcccatg atagcggcag ataaggtaga ttttcctgtg ccgttaggtc ccatgatggc
    17221 atggatttct ccggttttca ttgttaggtt tacgccttta aggatttctt tttcctcgat
    17281 cgacacatgt agatttttta tttctaagac agacatgcat tataccccca aattctcttt
    17341 cgttctcact cattttagac taattgagaa tgaagcgcaa tgaccttcct tactaaaatc
    17401 aaatatcttt tatttattat taaacaattt tgatttctaa attagaatag gtataaaaaa
    17461 gatgagaaat aagccattac agacttattt ctcatctttt ttgttccata ccttattttg
    17521 catcagcctt tactggttta acagagccgc cccatttgtc tgtgatccat tcttggactt
    17581 ctttgcggtg aagcacttca atcagttttt tgattttgtc actgtcttca tcacctttac
    17641 gagtcgcaat aatattcacg taaggagagt tgtccttttc aagcaatacg gcatcttcta
    17701 atggattcaa ccctgcgccg taagcgaagt ttgcgttgat tgcaactagg tcaccttcat
    17761 cattttggta cgtagaagct aaaagtgatg ggtcgatcgt atggttgaac ttcaagttct
    17821 tagggttttc ttcaatatca tcgaatgttg ctgtttccag atctactcca tctttgactt
    17881 tgactaagcc agcatcttgt agaattgtga tgatgcgtcc ccagtctgat tcagaatttg
    17941 aagtaatgat tgttgcacca tcttttaatt cggaaacatc tttgactttt ttagagtaca
    18001 agcccattgg ttcgatgtgg acagcaccgg cattgacaaa gtcatatcct ttttctttga
    18061 tttctttgtt caaataagga atatgctgga agtaattggc atcaatgtcg ccttcttcca
    18121 aagattggtt cggtaataca tagtcatcga atttgacgat ttcaagatcg attccttcat
    18181 ctttcaataa tggtttcaca tgttccaaaa tctctgcgtg cggtgtcggt gaagcgccga
    18241 ctttcaatgt cgtgtcgtct gctttatttg ctgaatcttt gctgtcgctg ttgttaccgc
    18301 atgctgctaa tcctacagtc agcaaaagcg ctgctgcaaa tccaaaaatt ttctttttca
    18361 tatcctacat ccccctatat cctattatct tttgtctgtt ttcttagtta agaaatcacc
    18421 gattccttga acgataaata cgattagtaa aataattaaa gtcgcaacga atgtcactgt
    18481 tagattacca cgctggtagc cttcctgcca agcaagacct ccaagtccac ctgcgccgat
    18541 cgcgccggcc atcgcagtaa atccgatcat cgagatcgtt gtaactgtta atccagaaat
    18601 caatgcggga atactttcag gtatcaagac cttccagatg atttcccagt aacttgctcc
    18661 cattgcatct gctgcttcca gtacaccagg gctgacttca cgaaaagcaa tctctactag
    18721 acgggcataa aatggtgctg cggataaaac aagagctgga atggctgctt gcgcacctag
    18781 catagtgcct acgattgctt tagtaaatgg aatgatcaaa accatcagga tcataaatgg
    18841 cgttgaccgg aaaatattac tgatgatcga tacgattcca taaatgattc gagcatacgg
    18901 tttgtttttg cgtccaatag agtaaaggat caatcccaaa actaatccga tgatcgtcac
    18961 taagaccatt gagattaatg tcatatatag tgtatcaagg atagcctgat tcatcgtttc
    19021 cgggttgatt tgtttaaaat ctagatatgt ttctgaaaag cttttatcca cgatggatca
    19081 cctcaattcc tactccttgt tctttgatga acacttctgc ttgatctaat tctttggttt
    19141 caccagtcat caagacagtc aatgaaccaa aggaaccttc tttcacttgt ttgatctttc
    19201 catacacaac gttgacatcg atgttatagc gtcgaatcgc ttgggaaatg actggctcgt
    19261 tggcatttct ttcgctaaag gtcaaagttg ccaaacggcc gcttggattt tctttgatga
    19321 gttcagctaa tagttctgtc gaatcagctt ctggttcgat gtcttgttgt acaaagcgtt
    19381 tggttacttc ttttttcggt tgtctgaaga cagtcaatac gtctccttct tctacgactt
    19441 tccccaactc catcacagcg actttgttgc agattttgcg gatcacattc atttcatgtg
    19501 tgatcaagac gatggtcaaa ttcaatttct tattgatcgc taacaataag tctaatactt
    19561 cttctgttgt ttgcgggtcc aatgcgctag tagcctcatc acaaagcagg atctcaggat
    19621 catttgccaa tgctcgtgcg ataccgacgc gttgtttctg tccgccggaa agttgtgaag
    19681 gatatgcttg tccgcgtcct tctaacccga ctaaacgcaa cagttcttct gcccgttctt
    19741 tacgtttgct ctttggtacg ccagctaatt ccagcggcag ctggatattt tcaagtacag
    19801 ttcttgacca taacaggttg aaatgctgga agatcatacc gattttcttt cggaatgtac
    19861 gtaattcttt gttttttaat tttgtaatat cttgatcgtt tacgatcact tcgccgctcg
    19921 tgggaagctc aagtccattc aggagacgga ctaatgtact ttttcctgcg ccagaatagc
    19981 cgacgattcc ataaatatcc cctttttcaa cgtttagtga aacgtcattg acggcttgga
    20041 tgtttccgtt tttcccattg aatgtctttt tgacattcgt tagttggatt aatgacatgc
    20101 cattcacatc tttctcttat gatttttcaa acaaaaaagc cttcgccctc ttgccttaag
    20161 caaaaggacg aaagcttgaa cttccgtgtt accaccttga tttaccgtta cttcgcaata
    20221 acagcctctt ccagtacttg gacttagctt ctttccgtat actgtcgcgc tgtaacgggc
    20281 actcccgtag taagtttgct tacgctcgct tactctgctc taagaccatc ttccatctca
    20341 ttttcgttac ccattttcag caaacagggc tctcttcaaa cgtccgtgac atgtactctt
    20401 cttttcaaag ctttctcgtt tcatatagga ttgcttatga ttatagcaac tggaagaatc
    20461 agtgtcaact tttttcaagt gattttctga ttttataatt cgtcaaattc ttttggatca
    20521 acatctttga aacttaagat ccacgcattc atttcatctg aatcattcaa gatattgata
    20581 tcttcaccga ttttatcatt aacagaagaa actgtacccg ttagcgggct ggcaaattca
    20641 ttgaccgctt tttccgcttc aacttcgatc agaggttcat ctttttttag cgattgtcct
    20701 gcttttggca gattggcaaa cgtgatgtct ccaagttctt cttgcgcttc atttgtcaaa
    20761 ccaacacagt attcttttcc attaaataag atccaaaggt tgtccttttt cttcaaatgc
    20821 ttgcctgcca ttttatatca gttctccttc ataaattttt tcattgaatc caattgcaac
    20881 aagctggtcc tctcgtaaga tcagtggtcg cttgatcagc ataccatctg tcgataagac
    20941 ttggctcgct tttgaagccg taaactcgcc tacttgatct ttcagtccaa gttcccgata
    21001 tttcatcccg cttgtgttga acaggcgacg gattggtact tgactcgttt ctagccaagt
    21061 ctcaatcgtt gaggccttcg gtacttcttt gatcatatca acggtttgat aactaacgcc
    21121 atgctggtct aaccacgctt tggctttttt gcatgttgaa catttcgggt accagtaaaa
    21181 ggtgtacaat tttcttacct ccttgaagat cttgcaggtg cttcattttg gtatctcatc
    21241 gataatatca acccgatacc gatcatgttc cctaaaatcg aggatcctcc ctgactaatg
    21301 aaaggcaatg ggatccctgt taaaggaagt aatccgatat ttgctccaat attctcaaag
    21361 acatggaaca gcagcatcat gatcaaaccg cttgcgatat aggcgtaaaa ttcattattc
    21421 gtatcgaaac acacccgaat catacgatag attaaaataa aatacaagaa aatgacgaat
    21481 gcaccaccga taaagccgaa gttttcaccg ataacggtaa aaatcatatc ggactcacga
    21541 acaggcacat agatattgct tacgttgaat cctttaccaa acaagccacc ggtaccaatt
    21601 gccaaaagtc cttgggccgg ctgataactc attcctgacg tatcatggaa agggtccagc
    21661 catgaatcaa tacgtgcaaa ctgataagat ttgaatccaa ctttataaag caagtctctt
    21721 ccgccttcag tcgtaaccaa gaagatcgtt cctgctccaa cgactactgc caagattact
    21781 acaggtgcga tgatgcgcca agagattcca gacatcagga aaatcccgcc aaagatcgca
    21841 agaaagacaa gcatcgttcc aaagtcatct tgggccatga tcaatgctaa aaccggtatt
    21901 gtcacgatca agagcttccc aatcagccaa aagtccgatt tcatcgtccg ttcttttact
    21961 tgcgtattat gtttagttac gatcagtgcc atcatcaaga tgtacgcaat cttcatcaac
    22021 tctgctggct ggaaagtaaa cgcaccgaat cgaaaccagt ttttagaccc tgttgaagca
    22081 gctaaattct gatcatagaa tttgagcaag agtcccatta ccactaaccc caaaccataa
    22141 aggtaaggag tcagtttcca gatccatttg gaattcaaat gcatgatgac aacgattgct
    22201 gccgctccaa ctaaatacca gacaacttgc atccctacgc ctttcaagac tgctggattg
    22261 ttcggatcat ggctaagtgc tacatataat gagagcagac caatcaaaca taacagaaag
    22321 actggtaaaa tcacgccata atcaatacga ttatcgttcg aagcttttaa ttttttttcc
    22381 atgtttcatc ccttttctta aaaaccatac tgctgattat tatagcggtc cagttagata
    22441 atggaaagtc tttccataga catttgggaa aaattaactt tccgtttgtg cagttgcttg
    22501 agcaaatgca gtacaaaagc ctactgctta tctttagtgt atcatagaac tttttttgat
    22561 acatgcaatt atctaccagc tgagattctc ctcgctgtga gaagtttctg tgctttcatt
    22621 gtatcgaagg atctcttcgg caatctctag cagctgatca gaaaccagat tggttcgttg
    22681 gatgaaataa aaatatcgat ggaaattcaa cggagtcatt ggcacatttt ctggaacaga
    22741 gcgcttagac aaaatcgagc gaccgattcc ttccttcagc aacgcaacaa ttacatcgtt
    22801 acttttcacg atcatcttct tatcttggat attcatctct tcaaaataac gctgcgtgta
    22861 atgaaaaaca ccagagtttg cttctcttac aagccacaat ttgctttttg ggtccccagc
    22921 gatgactagc tggtcttcca tcaaagagta tctttgtacg ccggaagtct ctaatggctt
    22981 ttcgataaaa ccaaaatcgg attcatgttt cgctacttgt tctaatgatt catgagaatt
    23041 cgtgatttcg atctcaaaat gcactttagg aaaccttttg atcagatgtt tcatcaactg
    23101 aggcaaaaga tagacagcaa aggtgtggga agcaacgatc cgacatactt cttcttcatt
    23161 ttgttcattg agcaattttt gctgagtttc ttcccattct tctaacaaaa ccagtgtccg
    23221 ttcatataat aagtcggctt gcttagttgg aagcatctct tttcttccgt tacgttgaaa
    23281 aagatcgata ttcaattccc gctccaactg tttgatttga ttcgagactg ctggttgaga
    23341 taaaaacagt acttctgctg ctctggaaaa atttcttgtt tcatatactg ctcgaaaagt
    23401 cgttaataac ttaaacatgg caccacttcc ataacttttt atgataatta ctataaaaac
    23461 tatttgattt aaatatacaa aaaaccatct ataatagaaa tgtacaacaa aacagttgct
    23521 gatccaacta gtaacttaga tgaagacaat cagctgtctg cttgctcgta atcatacatt
    23581 cctattcatt ttacaatgta ttcgttcatc ttggtactaa aatctattaa ataaaaagga
    23641 gaatattata tatgaagaac tttttgaaaa ttattccgat cccgatctgc gggttgatcc
    23701 ttgggttagc ttcccttgga aatctgttta aagactatca ccatgttgct ttaggcaatc
    23761 taactggcgg tatctctatg attcttatga tattcatttt tgttaaattg tttatcttat
    23821 tcgaacacac gaaacaaacg ttgcatgatc caatcatcgc ctccgtttca ccaactttta
    23881 cgatgtcact catggttatt tgtacttatt ttgtttcata tgaaagtatt gcaccaattg
    23941 tcaaattcat ttggctgtgc gctgttatct tccaagtgat ccttgtcttt tatttcaatt
    24001 atcaccatgt tatcaaagct gatctttcaa ttgaagcaat ttatccaagc tggtttatca
    24061 tttacgttgg atttggggtc atcactgtaa ctgccgggaa cttcttccca cttattggaa
    24121 aaattttctt ttggatttca ttgatctgct atgcgttctt attgccgatc atcatcaaac
    24181 gtgtcttttt cgtaagaaat atggctcatc cgacacgtcc gctgattacg atcgttgcag
    24241 cacctggttc tctttgccta acaggttatc ttaaaaactt tgctgaacct aatttttatc
    24301 ttgtcgtagc attgttcgct ttatctcaag tattgtattt cgttgtgctg tctatgcttc
    24361 cacgtttgtt caagcttcgt ttttatccaa gctatgcagc ctttacattc ccgcttgtta
    24421 tctctgcaac agctatattt actactgttc attacttcaa cagtttaggt atctatagta
    24481 gcgtattgga tttcttaaaa gttgcggaat gtctgtttgc aagtgtagtt gtcttctacg
    24541 ttcttgctca ttacgtacgt ttcttaatta aagaacatag caaacatgga aaatttgttt
    24601 ctaaaaaaat cgcttaataa tcgattcaat aggttgtgaa aaagctgttc tgcatcaagt
    24661 aataagaacg gccaaacaaa aacagctgtt catgtttttg tactggttgg gcttattacc
    24721 gcagatgcaa gcttttgaac accgtttacc aagaggtcgt aaaagaagtg gtcagcttca
    24781 agaaataaga aggaatttac gaaaattgct tctcaaattt ttgtgaaatt tcggcttatt
    24841 tccgtagaag ctacttctgg aacaccgttt attcgaaaca agaggtcgta caagactttt
    24901 gtcacgacct cttgttcttt tgtcaaaata ctggtttagt gctactatgt tcgagtatgc
    24961 ttttagtatt tattgacgaa aggaaccgat caaatgaaat cattgtcaca tccaataaaa
    25021 tctgtaaagg attcagcggt ccgcgaaatc ttacccggtt tactgctttc ttttgccatc
    25081 gctgccttca gcaggcttct tgctatttat ctgccacagt taggtggagc gactattgct
    25141 attttattgg gtattttatt aggaaatact ttttttaaac agcctgttct agcaatcggc
    25201 acaaaatttg ctgaaagcaa actattagaa ttctctgttt ttttactagg tgcaactgtc
    25261 acagctcaaa cgattgctca aattggtgta aaaggaattg tttttgtgtt actgcaaatg
    25321 tcagtcacta tcatcggtgc ttacttgatt ggtaaaaaac tgcaattcag cgatacttct
    25381 tctttgatga tggctggagg aaacgctgtt tgtggttctt ctgctattgc ttcaatcgcc
    25441 ccttccatcc atgcgaaaga ggaagaaaaa ggacagatca tcacactagt caatctactg
    25501 ggtactgtga tgatgttgac tctccctttt cttggattac agttgtttgg tggaaatctc
    25561 attgaaaaaa gtgcactttt gggtggtaca ctccaatctg ttggtcaagt agtcgctggg
    25621 gcaagcttac ttgacaattc tgttgtcgaa ttcgccatgc tatttaagat tctacgtatt
    25681 atgatgctcg ttgttgttgt ctatctgttt gaaaaattca tgcacaagaa acaaccagct
    25741 ctgtttgaaa cgccaactga tacgcagaaa aaacgaaaat taccgcttcc ttggtatgta
    25801 ttagggtttg tgatcttttg tttggtcaac agcttgtttt ctttacctgt agttattgac
    25861 caatcggcac attttatcag cagctggttt gagatcactg ctttagcggc aattggtctg
    25921 cgtttagact ttgcaaaatt catgaaagaa ggtacacgct tcttatctta tggattagct
    25981 gtcgggcttc tccaaacagt gatggcttta ctatttattt ggctgatcaa agtttagaat
    26041 gaacatgaaa aaacagttgt gattctcgca tggaacaatg tccgaatcac aactgttttt
    26101 aattttgcag atatccttct aacaacaaca attggcgttt gatttcaacc ccatctattg
    26161 cgtttcccat atatcctgtc agttttccat tttgcccgat cactcgatga caaggaataa
    26221 cgattggcaa tggattggtt cgatttgcct gcccaatcgc tcgcacagct ttcggactat
    26281 caacagcaac ggctacttct ttatacgttc ggatttctcc ataaggaatg gtcgacaaag
    26341 ctttccagac attttgctga aaaggcgtac ctctctttat agataaagga acatcaaatt
    26401 cttttctttt tcccgcaaaa tattcttcaa actgttcttg tgtgcgcgtt aaaattcgct
    26461 gattcgctga ttcgctttct gaaattggct gaaaagatac agcagtcaat ccggattcat
    26521 ctgcatctaa ccataaaaag ccgataggtg tgtgtatttt cattttgttt gctcctagtc
    26581 aatagactgg aacttcctcc tttttctcta tccaaaacta cttgccaact aataaaaaat
    26641 gttcttaaaa gtttgataaa ccgaggaaac atggtaaact aaatacgtta gtttacaaaa
    26701 agaatacctg ttcttttacg taaaatcaaa ttttaaaggt gttatttaag gtttattaaa
    26761 ttttgaatgt aaaccatata taaacatacg attgaaagga tagaaacgat gaaagtatca
    26821 attgttgtcc catgttttaa tgaagaaaaa actgttcctc ttttttttga tgaggttgaa
    26881 aaattcaaag gtgcccatga gttcgaatat ttgtttattg acgacggatc aaaagacggt
    26941 acgttagcag caatcaagaa gctagcctca gcttatcctt atgtccgctt tgtctcattt
    27001 tccagaaatt ttgggaaaga agcggctctg tacgctggat tgcaagcttc tacaggagat
    27061 ttagtgaccg tgatggatgt tgatctgcaa gatccgcccg aactgcttcc tgagatgatc
    27121 aaccggatca ctacttctga tatcgattgt gtcggcaccc ggcgttcgac tcgtgaaggc
    27181 gagcctgcga tacgcagttt ctttgcgaaa aaattctacc agttgatcaa taaaatcagc
    27241 gatacagaaa tggtcgatgg cgctcgtgat ttccgcttga tgactcgtca aatggtggat
    27301 gccgtcctag aattaacaga atacaaccga ttttcaaaag gattatttag ctgggtcgga
    27361 tttaaaacag aatatatttc ttatgaaaac cgcgaacgtg tggctggcga aacatcttgg
    27421 tccttctgga aattattcaa ctactcgatt gatggtatcg tgaacttctc ggatgcaccg
    27481 ttaacgattg cttcatttat cggcgctttg tcttgtgtcg gttctggtat tgcaattttg
    27541 ttcattattt tgcgggcatt gttgtttggt gatcctacat caggatggcc ttccatggtc
    27601 tcgatcttcc tgttcattgg tggaatccaa ttgttatgtc ttggaatcat cgggaaatat
    27661 attgggaaaa tcttcttaga aacgaaacag cgtcctgttt atatcataag agaaacagaa
    27721 aaagtgaagc caaatgaatt gaagcctcac caataatata cgtcaaatga agtaaacatt
    27781 tttttaaatg aaaaaggact gtgacaaaag tcttgtcaca gtcctttttt ccgaataaat
    27841 ggtgttccag aagtagcttc ttcggaaata agccgaaatt tcacaaaaat ttgagaagca
    27901 attttcgtaa attccttctt atttcttgaa gctgaccact tctatcacaa cctctttcat
    27961 atcttattac ttcttcattg ctgcttttag tgctgctgct aatgggcttt cttctggttc
    28021 ttctgcttgt gcatattttt tcagcaagcg tcgttcttca tgtttggtca ttttcttctt
    28081 ctgctctttt ttatcaagca atttttctgt cgttccatcg tatttacaac ggaaataagc
    28141 accgttttta ccttcgatga tttccatttt ccggtgacat tgcgggcatc gatgattaga
    28201 gacttttgga tcttttcttc tgcggtagtt gcaatctgaa ttggtgcaga catagatttt
    28261 gccgtcacgt gtgtttttct cgcgcagatt ggccccacat tctgggcatt tcttttgggt
    28321 aatcgagaaa tcttgatatt cttgtttgct tgatttgatc tcattcacca agcgttttgt
    28381 ttctttttcg attccattca agaaggttcc tgcttgtttc tttcctgcag caatagcttc
    28441 aaggtctttt tcccagcttt ctgtcaaatc tggtgttact aaagagggat ttaccaatac
    28501 taataattgc ttacctttag gagatacttg cagtccttga ggggtacgtt ccatcagttc
    28561 agatttgatt agtttctcga taatctctgc acgcgtagct ggggttccca gactgtattt
    28621 ttccatttga ccaagcaagc ttgcttctga caatggttta ggcggtgcag atagttcttt
    28681 tttgatggcg aaatcgggat tgattctcat tcctttttcc catttgactg tttccgtctc
    28741 agtttcagtt tcgcctttcc atcctgcttg gacgacacga ttttgccgaa aaatgaatcg
    28801 ttctttacca aattcaaccg taacttttgt ttggctgact ttatggggtt gagcaaacag
    28861 tcctaaaaaa cgagagacaa tcatttgata gatttttcgc tcttcattac ttaatttttc
    28921 ataacgtgct ctgttttcag ttggcaaaag tgcatggtga tcggtcactt tactgtcttg
    28981 gaatactttt gtctgttgga cgatggctcc gtttttcaaa tactgcttga cctcaggagc
    29041 aaagtcgctg actgcttgca atcgttcttt catcgtactt ttcatgtcat ttgtcagata
    29101 tttgctgtct gtacgcggat aagtaacgat cttatgggtc tcatacaaac tttgtacaag
    29161 acttaatgtt ttctttgcag aatagccata gcgttgattg gcttctcttt ggatctctgt
    29221 taaatcataa ggcaatggcg cagattccgt ttttaatttt tcttggactt ctgtgacagt
    29281 tcctttgctt gtactgatca attgaacaag tttttgtgct tcttcttgtg tttttaaagc
    29341 aaattgattt ttttgaagca ttttggcttg ttcttttcca gcttgcaacg tgatcgtgaa
    29401 atacgtttgt ggacggaact gctcgatttt cttttcttgg tctctcacta atgctagtgt
    29461 cggtgtctgc acacggcctg ctgataaatt atccgcgtac ttcacagtta aggctctggt
    29521 cacattcaat cctaccagcc agtccgcttt tgcccgggca agagcagatt catacaatgc
    29581 atcatattct ttcgcaggtc ttagttgctt gaatccagtt ttgattgctt tatctgtttg
    29641 agaagagatc cataaccgct tcactggttt attgaaatgc acatactcta agatccagcg
    29701 agcaacaagt tccccttctc ttccggcatc tgtcgcaatg actgcttctg agacatcttt
    29761 tcgctgagcc agctgcttga ttgcttttaa ttgatgtcct gttttaggaa gtggtttgat
    29821 tcctagtttt ttagggatca ttggcaacgt gtccatttgc cagctctgcc attccttatt
    29881 cagatcttcc ggcattttca atcctaataa atgaccaagg gcccaagtaa cgatcacctt
    29941 gggtccttca taataatttt tgtgcttttg attagcacca agcaccttgc ttaggtcttt
    30001 cgcaacgctt ggtttttccg ctagaattag ttgtttcata aaattccttt ctgctttcta
    30061 ctgtgatggg ctgatgattt ttcaacaacg caagtccctt gtattgctgt aaatcatcgt
    30121 cacactcttc acaccagcgc tcatctccgt cattccaaaa ggcgctgaga aagttacttt
    30181 ttgcattgat atattcataa ttttgatgga tttcgttcca ttgtttttcg tacgcttgtc
    30241 gtgcttcttc gaaagactcg aatgtttctt cttcttcaat atcttcttcc cagtcttcaa
    30301 aaaaccacca aggctcattg tccccgaaca tggtaattac ttgatacata ctcgtgctcc
    30361 ttctttcagc ttttctagac tcatcttagt cacttttcaa gaaagttgca agaattttct
    30421 cttataaaag ggtcatttaa tgaacatctt aaaattgtta caaatgaatc tgattgttta
    30481 aggcaaagta gcgataaagt tattgatcat ccaaaaatag ctgtcttctg gatcgattgt
    30541 tggttctttt gtaaagtatc ctaaaaaacg taaggaaaga taaccgtgga tcatactacg
    30601 aagttccctg cttcgatgaa ctttttcttc tttcgttaat tgaaaagcct caaatatctg
    30661 caaaatgatc tgattcgttt catggattcc atctaataaa acagcttcat tggtatgcgg
    30721 tacactgatc agtaactcat acactgcttg atttgaaaaa gcaaaggatc gataagtttc
    30781 tgcatagatc tgcagtgctt cctgaccact ttttcccaca agagcccgtc gcagctggtc
    30841 attaagttcc tgcagaagtt tcgctgttag cgctgttttg acatcacgaa tatttttgaa
    30901 atggttatac agcgagggag ccttgatatc caagcgtttg gctaaatgct gaaaggttat
    30961 ttgctgaaca tccatctctt ctgctagttc tcgaaagcag tcgatgattt tctcttgaga
    31021 cagatttctt tttttcattt ccatgtttcc tctcataaaa taaatccttc actctttcag
    31081 tttaccacga aaaagaaaga gaaaaaagag actttcattg ataaactaca tattgtagtt
    31141 tataataaag agctgtagat gaaaattttc agaaagaagg agtctaattg gctgcaagaa
    31201 taaaggaaaa attatttagg aaaagagaaa cttgggaaca aaacttgttt gtcttatggt
    31261 ttggaacatt tatggctggt atcggattta gtttagtcat gccttttatg tcactttaca
    31321 tagatacgtt aggtaattat agtacgtcac aattgaactt ttggagcgga ttgacttttt
    31381 catctacttt cttggtgact acgcttatct ctccttggtg gggaaaatta gccgaccaaa
    31441 aaggaagaaa attgatgctg ttgcgtgctt cattaggaat ggcaatcgtc atttctttga
    31501 tgggttgcgt gactagtgtg tatcaattag tcgcattgcg tctgcttcaa gggatttttt
    31561 ctggttatat cagtaatgcc actgcccttg tcgcaactgg gacgccaaaa gaaaaaagtg
    31621 gtcaagttct tggaacctta gcaactggtt ctgttacagg aacattactt ggaccgcttc
    31681 ttggcgggat ctctgcttca gcgtttggct atcgccctac ctttttcatt acagggatca
    31741 tcttattttt cgtcttcttg ttgagtctgt ttttcgtcca tgaaaaattt gtcccagtga
    31801 aaaaagaaaa catggtttct gccaaacaga ttttcaaaga cttgaaatat ccacacgtcg
    31861 tcatcggtat gttcgtcaca acgatgatca tccaagcatc caacaattcc attagtccaa
    31921 tcatcagtct gtacattcgc caattgctgc atggacatgg gaatgttacg ttgatcagcg
    31981 gaatcattgc ttcgatacct gggattgcca ctttgatagc cgctccaaga tttggtcgct
    32041 taggtgacaa gattgggagt gaaagaatac ttgcgattgg tttgggtttt gcgattctgg
    32101 tttatatccc aatggcattc gtcacgaatg tttggcaatt agctgcctta cgttttttga
    32161 ttggtatctc tgatgcttgt ctgctccctg ctgtccaagc attgatcaca aaatattcgc
    32221 ctcatcaagc tgctggacgg atcttcagtt ataaccaatc tttccaagca actggtaacg
    32281 tagtagggcc tatgatcggc tccagtgttt ccagtgcatt tggttatcgt ggcgtctttc
    32341 tttcgacttc tcttcttgtt ttggtcaact tcttgcttgt acgacacaat acacaagaga
    32401 tccatcagca agaaactgca gaagaaaaag cttcaggagt ctccatagct tcagggcact
    32461 ctccttctca tagacaataa gcgtaaatga aaaacactgc ttgaacagtt gtcacagact
    32521 gtccttgcag tgtttttgtt taggcctgtt ttttcaagaa ctcagataag aggaataatc
    32581 ccttttccaa ggtttctttt ttacaacagt aacctagtcg ggcatgcttc ggtaaatcaa
    32641 aggcacttcc tggtactaat agtactcctg tttcttctaa taaattgata caaaatgtct
    32701 ggtcatcttc ttcgatggct aatttaatga aagaagtaga gacgtaattg ggagcgacaa
    32761 gttcaacttt cggttcattg gctacccatt gttttaaaat cacaaggttc tctgtaatga
    32821 tctttttgtt tcgcgccaat attttttctt tattcttcaa tgcatgtaca gcaagatcat
    32881 ctgacaggac tccgccgcaa atcatcgtgt agtctcgata ctttcgaaaa acttctgcca
    32941 actcaggtcc agtagctgtc cagccaatac gaatacctgg aatggagtag gttttggata
    33001 gggaattggt tgctatacct ttttcataga catctacgat cgacaagaat tcacctttat
    33061 ccgttaaagg cgcatatact tcatctatca atacatatgc atctactgta cgagcaattt
    33121 ctgcgatttt ttctaaagtt ttccgatcta gcaatgtgcc tgtaggattg tttgcactat
    33181 tcaaacagat gatttttgtt tcaggtttga ctaaggcttc tagctgttct aaatcaaact
    33241 gccaatcttc ttcttccttc aaatgaacaa agtcaacagc ggcacctaaa gatttgggga
    33301 tgtcatataa ttgctgataa gtaggaagca tcgaaacaac atggtctcct gcttccacta
    33361 acgcgtatag agctaaaaga ttagcaccag tcgctccatt cgtttgtaag atgttttccg
    33421 gatcgacggt ttgataaaga gaagcgactt cttgtttgaa aacatctgat ccttcgatcc
    33481 agccatagtc caatggttcg tttttgcgtt tttcgaaaaa tttacttacg ctagtcccat
    33541 ctaagccaac gagttcgtct aatgtcaatg cttcgatcga gctttgtgca atatcatata
    33601 ccgctttctt ttcccattta tttagccatt cttctacacc gaaatttgcg attttcatgt
    33661 ttctttcccc cttatatctt gatccagcct acagcaagca acccgattga aagcagtgcg
    33721 gccacacaaa tcagcccaat cattactttt tcttttcccg taatttgatg tttggtttca
    33781 cgacaagcga tccagtagaa gcaaaaacca ggaagatagg caatactaca taaaaggaca
    33841 taagaaaatc ctgctaaaat gatgcccatc aattcaaaaa ctacagcaat cccaccgatc
    33901 actacttgtt ttttgattcc ttcttttaaa gaaaactgga cttgatagat cccgacaagt
    33961 agataacaaa tcaaaattgc cgctgtacat aaagaatagg caaattgata ggcataatca
    34021 gtaactaaga aagtcaataa gaatagatta gtcaagattg cagtaacaac taacgaataa
    34081 gttggggcat tttttttatt cgtttttccc caacctttcg gcaacagacc gtctcgagcc
    34141 atcaacatcg ttgtttctgc tgggagcatc gtccaagaca accaagaacc aatgatcgaa
    34201 ataatcaagc cgatattgat cagcgctgct ccccaatttc ccacgattcc ttttaaaata
    34261 tttgccatag caggttgtga aatagttgct aactcttctt gagataagac gccgtatggc
    34321 aagatcgaag caaggatata gataaacaat aaacaaagca gaccaatgat cgatgcttgc
    34381 tgggcttctg aacgtttctt cgcgcggttg gctaaaacac ttgcgccttc gatccctaca
    34441 aagacccaca tcataaccat caaacaacct ttaatctgca accagatcgt tccttgctgc
    34501 cctgttccat tcactagatt atcagatacg ttaccccaaa aatcagcagt gaatatattc
    34561 actttaaatg cgataaatac aaatattaaa aataggaata atggcactaa tttacagatc
    34621 gtcacgattg tattgatgac gctcgctcct tcaactccat tgtttactaa taaagtcaga
    34681 ccccaaataa aaatacttgc aaataaaata gaaggaacat tttgtccacc tttgaatatc
    34741 gggaaaaatt ctcccatggc gctcatcagc atcgttgcaa aagcaacatt tcccagccac
    34801 gcagacaacc agtaacccca accactgatg aagccaccta atttaccaaa tgaagcttcc
    34861 gcgtaaccga aaataccacc atctagatct ggacgctttt ctcctaaatg attcagcgat
    34921 aataccaaag ctaaaatacc aatcccaacg attatccatg aaaggatcgc tggccctgga
    34981 gctgcagaac tagccagatc acttgtgatc ccaaacacac cgcttccgat tgacgaactg
    35041 acaaccagag caaccagtcc ccatttgcta atacccgtcg atttcttttc catttccatc
    35101 accctcactt ataaaattga acaaatcact tgcaaaagat tcccttctat tttgcaactg
    35161 ctcttgctta acaaaaagca actttattca tattattgga tattatgaac tttttacaca
    35221 agtatttcaa gtataaaatg aataaaaatt aattttattg caaaaaaaca gctatgctat
    35281 ccaaaagaaa aggtgtttct tttgttagcc atagctgttt tgattcattt aatatcgctt
    35341 aaacgtattg taaaactttt gtttcttgat atgcgtggtc gatcaaacca ccacccagac
    35401 attccattcc atcataaaat acaactgctt gtccaggagt gattgcacgt acaggttcgt
    35461 cgaatacgac ttctgcacga ttatcatcca ataagcgaac tgtcacagga acgtcttgct
    35521 gacgataacg gaatttcgct gtacacttga attcttttgg cattggttca ttcgtcgtaa
    35581 agtggatttc acttgcatcc aagcttgtgg catacaatgc aggatgatgg aacccttgcc
    35641 ccacatataa tgtgtttgtc gctagatctt ttcctacaac aaaccagggt tcttgcgaat
    35701 ctcctccgcc accgatccct agtccttgac gttgaccaat cgtataatac atcaagccag
    35761 catgttgtcc tttgacttca ccatcaagag tgaccatatt tccttttttt gctggcagat
    35821 agttgctcaa aaattgtttg aagttctttt caccaataaa gcagattccc gttgaatctt
    35881 ttttcttagc agtcgcaagg cctgcgcgtt cagcaattgc ccgtacttct gatttttcca
    35941 tgccgcctag agggaacatc gtttttgaaa gctgttcttg tgataattgg ctaaggaagt
    36001 aagtttgatc tttattattg tctactccac gaagcatgtg gaccacgccg ttttcatcgc
    36061 gagtgacttg cgcataatgt ccagtagcta cataatcggc gccaagatcc attgcataat
    36121 ccaagaatgc tttgaatttg atttctttgt tgcacatcac gtctgggtta ggtgtacgtc
    36181 ctgcgcggta ttcggctagg aaatattcaa atacacgatc ccaatattct ttttcaaaat
    36241 tcacagagta ataaggaatc ccgatttgag cagctacttt cgctacatcc ttataatctt
    36301 ccgtggccgt gcatacaccg ttttcgtccg tgtcatccca atttttcatg aatattccga
    36361 tcacgtcgta tccttgttcc tttaacaaaa gtgctgttac agatgagtcg acaccgccgc
    36421 tcatccctac gacaacgcgt gttttgctgt tgtctgtcat gatatcacca tcatttcaat
    36481 tagattctga aacatctacg agttgaaggt cgcaactttt cagtatttta cattatacgt
    36541 catatttctg aaaatttcat cccttaatta tcaagtcgta aaaatactgt tctactggac
    36601 ctattcagta cacaaacatg atagtcaacc aaaaaatcac tcatcttttg agcattcgat
    36661 ataaaaaatt ggcatactca taaaaagggc cagcctttcg gcaataagga ataattttat
    36721 aaattgtgaa aaactatttt tcaaaattgc ttcttatcgc ttaaagctgc acgtctcatt
    36781 tacaaccttt tctttttaga tgttatgagt tgctcagata agtgattttg tttactctaa
    36841 gaaaaaaggg aggcaaaaga tacatgaatt actttatcag tgatatgcac tttttccatg
    36901 aacgcttatt aggaaaaaac gactttgctc cgcggctctt tgcaaatgtg gatgaaatga
    36961 accaacagtt gatcactagc tggaatgcag taataaaaga aaccgaccat gtttatcatc
    37021 taggcgacct tgctatgcat ccgcaatatg aaaaagcaaa tgcagaaatt ttggaactgg
    37081 tcactcaact gcaaggaaca atccacttga tcaaagggaa ccatgacagt cgcgcttttt
    37141 tccgttattt agaaaaaaat gatcctaggc ttactgacgg aagtcccaaa ttttattttt
    37201 atgatgtagg tgccatcgtt aaattcgacc atcaacaata ttacttgact cattatccga
    37261 tgttgctcgg gcctaacgac actatccgca atcttcatgg acatatccat cattacagcg
    37321 tacctatcga gaacgatgtc aatgtaggcg tagacgcacc ggaaagagaa ctcttaaagg
    37381 aaaaactacc atttggcact ccgttatctg agcaacaaat caaagaaatc tatgaagcaa
    37441 agaggaagta cgataggtcg tgaaaaaact gccctatatc aagtattaaa caaataaaga
    37501 taattactca tatttttcat ttgagtaggc ttattgccac aggaacatta ggatcagaaa
    37561 tggagttgta acaagacttc tgtcacaact ccattccata ctattcgtct ttttgctcat
    37621 ggtgttcttt taccagtttt ttctgtaatt ctttatcata atttcgggca gctgacgtta
    37681 aaaataaaac gattccgcat aataaaatcc cgattccgga aaacaagaac attttttcta
    37741 cccctaaacg atcagctaat ggtcctgcaa ataataatcc aattggacct gtcaggttca
    37801 tcaaagagtt gaagttccct aagacacgac ctaaaacatt cgattcataa ctctgctgga
    37861 tcattgccat cagcagtgta ttgaaatatg gcgttgcaca acctgctcct gcattcaaga
    37921 taagaaagta caagaaccct tgactatcgt ttggaaccaa tccgcttgca ccgatcgtca
    37981 ggcccataat gatgtaggct ataataatag gtttcattcg gtctttccat gttcctataa
    38041 aactgatcag tgcaccgcct aagagcattc cgactgcgta gactacttcc actgctcccg
    38101 cctgtccaac ggaaccacca aagtaatcta gcgtcatcaa tggatacaga ctgattgctg
    38161 gcatgaacaa cagcatcaca aatgcaccat tgatcgttat gtaccataac cctttattat
    38221 ccatcaattg ctgcagccca aattttgcat ctttaaaaat agtaagcgga acttccaata
    38281 tttcaggaga aacttttggg atcttcacaa aaatcaataa gccgacacca aaaactgccc
    38341 ccaatacatc taataaaata aggtaattga caggaacgac tgaaaacaaa gccgcaccta
    38401 gagctggcgc aatgatgaaa ttcgctgatt ggaccattcc taactgtcca ttcgtccttg
    38461 ttatgtgaga agatggaacg attgtcggca taatcgactg gatcgttggc atttggaatg
    38521 tttgtgcgac cgaacgcata aacaaagaca caaaaactag ccaaatcggg aaggattctg
    38581 aaatcgttcc gactactgct aaaataagcg cgaaaatcgc tacaataata tccgtgacaa
    38641 tcaaaagagc ttttttatcc cagcgatcca ctagcggtcc tacaaatggg cttagtaaaa
    38701 ccatgggaat cattcctagt aaagtagcaa aactcaaaat tgtcgccgaa ccggtttctc
    38761 ttgtcaaata ccagatgatc gcatattgga cgaccatact agtgattcct gacaaaaact
    38821 gtcccgataa aaagaacaaa taatttctct tccaattcgt taaaacgacc gactgttcat
    38881 tttccataaa catccctctt ttctttttca ttttgtatag cgtttgcaca acaatcatta
    38941 tacgggattc ttttttcaaa aaataccttt ttccctaaat aacaatcgtt tcaatttaaa
    39001 tttctttact tagaaacaat aaatcagcaa aatattaaaa ataacaacat gtgttaaatt
    39061 taaaaataaa aaagacaact aatttgcgtc gtccttacaa agttagtcat ctaacaatag
    39121 gccgtttcaa aactagttgt ctttttatat ccacagatca ttcgactttt tcgaataacc
    39181 cacggctaat catcccatcc atcagaaaat aaaatttatc ttggctcatt ttctgttttg
    39241 gatctttgaa aatattgact gctttcaatc catttgctgt agtgatcgac attttcaatg
    39301 tttgaatatc ttttgataca gtgattttta atttgaatcc ttctttgctt tgcggcgtgg
    39361 catctaatgg tcggatcaat tgataattgc ctccgtttgt ttctgtaaat ccattatcgc
    39421 gcaatgtata tctttttgca tcaggatgca aacgatagct aattggtgcg cccagtaccg
    39481 ttgctgttgt ttcaaatgcc atgattttca cctcgataaa attagtttct ctttattata
    39541 aaagtttatc ctgattttta ctaggttttt cagttaagtt ccctaaaaaa tgaaggcgtt
    39601 ctctcagaca tctgttcata taagaatgac ttcactagtt attttttgac agcacgaact
    39661 aattgatcaa ggaaatactg gatgtcgtct tcggtatttc ccaaaccaaa gctgatacgg
    39721 atggattctt ttagtatcgg tgtatcttct ccatacattg cttccaatac gtgtgaaggc
    39781 tcgatatttc ctgctgtaca agctgaacca gtagaaatag cgaccccatt aagatcaagc
    39841 ttcatcagta gcaaatcatt tgcttcgcca attaagcgaa gattcaacac atgagggagt
    39901 ttattcgtta aatcaccatt tacttgataa gggatttcgt ttgcttctaa tgtatcaaga
    39961 atcaattgct ggaatccttt atatttgttg ttacgtgctt cacgttcttc aggtgacagc
    40021 agttcaactg cttttgccat accccagatc cctgctaaat tttctgtacc tgcacgccgc
    40081 ttttcttcct gctccccgcc taaaagcaat gcagggatat ttactccgtc tcgtttatat
    40141 aagaaaccga cgccttttgg cccattgatt ttatgagcag agatacttaa taaatcgatt
    40201 cctaattctt ctggatggat cgtttggttt ccgtacgctt gtaccgcatc tgtatggaaa
    40261 taagctggat gatccgctaa aagttcaccg atttctttga ttggcatcaa atttccgatt
    40321 tcattgttcc cgtacatcac agagaccaag atcgtatctt ctcgtaacgc ttttttgacc
    40381 tcttcgatcg acagattccc tttttcatct acaggcagat atgtaacgtc aaaccccttt
    40441 gtctctaaat atttcatggt attaagaaca gcaggatgtt cgatagctgt agtgattaga
    40501 tgtctgcctt ctttttgacg cgagaacgct gtttctaaga tagctgtatt atccccttct
    40561 gttcccccac tgttaaagat gatctcgtga ggttttgctt gcagactgtc tgcaatgatt
    40621 tgtcttgctg tttccaattt ttcatgcgcg tggcgaccaa atccatgtat gcttgaagga
    40681 tttccaaacg tgtcttgcat gattgccgtc atcgcttcaa ttacttgtgg atgcatgggg
    40741 gtagttgccg catgatccag ataaatagtt ttcacttcat tcatcccttc caaattattg
    40801 ctaggcacat tctagcataa aatagcgaac aaaccaaaga agagctcatt tttgtctaga
    40861 ggacgtttca tgggcataga aaaaaggagc tgtgacaaaa gtcttgtcgc agctccttga
    40921 agctgctcac ttctgtcaca actt
//
LOCUS       DAZUKZ010000015        40414 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000015 DAZUKZ010000000
VERSION     DAZUKZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.015"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="ACG8EF_000748"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="ACG8EF_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFS4946097.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="ACG8EF_000749"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="ACG8EF_000749"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFS4946098.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="ACG8EF_000750"
     CDS             complement(2303..2965)
                     /locus_tag="ACG8EF_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4946099.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="ACG8EF_000751"
     CDS             3149..4795
                     /locus_tag="ACG8EF_000751"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS4946100.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="ACG8EF_000752"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="ACG8EF_000752"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4946101.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..7529)
                     /locus_tag="ACG8EF_000753"
                     /pseudo
     CDS             complement(5976..7529)
                     /locus_tag="ACG8EF_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7677..8818
                     /locus_tag="ACG8EF_000754"
                     /pseudo
     CDS             7677..8818
                     /locus_tag="ACG8EF_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8944..10023)
                     /locus_tag="ACG8EF_000755"
     CDS             complement(8944..10023)
                     /locus_tag="ACG8EF_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4946102.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10126..10566)
                     /locus_tag="ACG8EF_000756"
     CDS             complement(10126..10566)
                     /locus_tag="ACG8EF_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4946103.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10623..11033)
                     /locus_tag="ACG8EF_000757"
     CDS             complement(10623..11033)
                     /locus_tag="ACG8EF_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS4946104.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11213..11647)
                     /locus_tag="ACG8EF_000758"
     CDS             complement(11213..11647)
                     /locus_tag="ACG8EF_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4946105.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11747..12595)
                     /locus_tag="ACG8EF_000759"
     CDS             complement(11747..12595)
                     /locus_tag="ACG8EF_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4946106.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            12984..14750
                     /locus_tag="ACG8EF_000760"
     CDS             12984..14750
                     /locus_tag="ACG8EF_000760"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFS4946107.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            14852..16111
                     /locus_tag="ACG8EF_000761"
     CDS             14852..16111
                     /locus_tag="ACG8EF_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4946108.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            16258..17559
                     /locus_tag="ACG8EF_000762"
     CDS             16258..17559
                     /locus_tag="ACG8EF_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4946109.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            17562..18413
                     /locus_tag="ACG8EF_000763"
     CDS             17562..18413
                     /locus_tag="ACG8EF_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS4946110.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            18426..19241
                     /locus_tag="ACG8EF_000764"
     CDS             18426..19241
                     /locus_tag="ACG8EF_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFS4946111.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            19392..20558
                     /locus_tag="ACG8EF_000765"
     CDS             19392..20558
                     /locus_tag="ACG8EF_000765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4946112.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            20788..21435
                     /locus_tag="ACG8EF_000766"
     CDS             20788..21435
                     /locus_tag="ACG8EF_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS4946113.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            21519..22463
                     /locus_tag="ACG8EF_000767"
     CDS             21519..22463
                     /locus_tag="ACG8EF_000767"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFS4946114.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            22598..24049
                     /locus_tag="ACG8EF_000768"
     CDS             22598..24049
                     /locus_tag="ACG8EF_000768"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFS4946115.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            24070..24906
                     /locus_tag="ACG8EF_000769"
     CDS             24070..24906
                     /locus_tag="ACG8EF_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4946116.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            24906..25565
                     /locus_tag="ACG8EF_000770"
     CDS             24906..25565
                     /locus_tag="ACG8EF_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4946117.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            25691..26380
                     /locus_tag="ACG8EF_000771"
     CDS             25691..26380
                     /locus_tag="ACG8EF_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946118.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            26386..27342
                     /locus_tag="ACG8EF_000772"
     CDS             26386..27342
                     /locus_tag="ACG8EF_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFS4946119.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            27386..27898
                     /locus_tag="ACG8EF_000773"
     CDS             27386..27898
                     /locus_tag="ACG8EF_000773"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4946120.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(28154..28702)
                     /locus_tag="ACG8EF_000774"
     CDS             complement(28154..28702)
                     /locus_tag="ACG8EF_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS4946121.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(28791..29972)
                     /locus_tag="ACG8EF_000775"
     CDS             complement(28791..29972)
                     /locus_tag="ACG8EF_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFS4946122.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(30208..31716)
                     /locus_tag="ACG8EF_000776"
     CDS             complement(30208..31716)
                     /locus_tag="ACG8EF_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFS4946123.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            31875..32066
                     /locus_tag="ACG8EF_000777"
     CDS             31875..32066
                     /locus_tag="ACG8EF_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946124.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            32269..33186
                     /locus_tag="ACG8EF_000778"
     CDS             32269..33186
                     /locus_tag="ACG8EF_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFS4946125.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            33179..34252
                     /locus_tag="ACG8EF_000779"
     CDS             33179..34252
                     /locus_tag="ACG8EF_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946126.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            34400..34918
                     /locus_tag="ACG8EF_000780"
     CDS             34400..34918
                     /locus_tag="ACG8EF_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946127.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            34899..35291
                     /locus_tag="ACG8EF_000781"
     CDS             34899..35291
                     /locus_tag="ACG8EF_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFS4946128.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            35367..36302
                     /locus_tag="ACG8EF_000782"
     CDS             35367..36302
                     /locus_tag="ACG8EF_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4946129.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            36435..37214
                     /locus_tag="ACG8EF_000783"
     CDS             36435..37214
                     /locus_tag="ACG8EF_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS4946130.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            37207..37524
                     /locus_tag="ACG8EF_000784"
     CDS             37207..37524
                     /locus_tag="ACG8EF_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS4946131.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            37768..39378
                     /locus_tag="ACG8EF_000785"
     CDS             37768..39378
                     /locus_tag="ACG8EF_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4946132.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            39535..40383
                     /locus_tag="ACG8EF_000786"
     CDS             39535..40383
                     /locus_tag="ACG8EF_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4946133.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        13231 a         7260 c         7799 g        12124 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
     6841 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
     6901 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
     6961 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
     7021 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
     7081 atagacaaac cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
     7141 gaggcactat agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac
     7201 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
     7261 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
     7321 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
     7381 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
     7441 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagata
     7501 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
     7561 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
     7621 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
     7681 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
     7741 agacagtcca cacagaggag acagaaacag ccaaatggac agaaaccaag tgggccaacg
     7801 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
     7861 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcgggagac ttagcaccta
     7921 tactacaatc agctgttaca tttgagaatg ttgatcccac tttgtcgatt ggctatgacg
     7981 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
     8041 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
     8101 atcatttaac gccgtatagt cagcggcaga caattgagca agattcagcc atcgactcag
     8161 gaggagatct tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac
     8221 cgcctacaga aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg
     8281 atacagaaac tcaaccagag ccagacaata aatcaaaacc tgaaataacc cctggagaaa
     8341 aaccagatgt aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa
     8401 atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagat ccagaagaaa
     8461 aaccagatgt gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa
     8521 taaacaaaaa aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa
     8581 ttctaaaaat aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat
     8641 cagctgaaac cttaaaaagt agcaaggata caacctttgc tgtcctgaaa cgaaaaataa
     8701 acagctacca aaatccggag agtcacaaaa caaagttata ctgtggagcg gaatcattct
     8761 attaagtata gcaacaatgc tttctgcaaa aagatttaaa caaaacagat ctctctaaca
     8821 tttgaataat gtctgtgaac gcatttttca aataaaaaga aatatccaga aaatgacccc
     8881 aaaataccag attgaaatct gatatttggg gtcgttcata ctttatcttt cttcatattt
     8941 attttaaggg atatctgttt cttttggaag atggttcggg atctttttca ctttcaatac
     9001 atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg
     9061 ttcgctaaca agtaagacca caaatgtata aaaaatcggc agttctgttt tgctggacat
     9121 caacttcgga ttcagcacat aagactcaat tagatggaca atcaaaatcg taatcaggat
     9181 ataaacaaca tctcggatgc ctccttggga ataagctatg aaagatagag gaatacaaga
     9241 aatgataaca cctgctactg ggatcagact taaaatgaaa atcatgatcg ctaaactcag
     9301 cagctgatga aagccaaata cagccaaggc gattgtagtg agcaacgtat ttaaaagagc
     9361 gacaacaaat tgagcttcta ataccaaacc aaatgtattg acaaacttat cggcaaaata
     9421 gtaaatatct tgaaagaacc aagaaaattc actttttaaa aacaatcttg agaatacaat
     9481 tgtttttttc ttttcaatca taaaaaagaa acttaagatg aatgacatag caaacgccac
     9541 tgccattttt cctatatcct gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg
     9601 ttcaaaaaaa tcgtttcgct caatataagt atggatatat tgtaaaacag gatctgcatc
     9661 ttttggctga tgctcgtaaa aattaataac ggactggatc atgtcaaatg tttgatgaat
     9721 aagcatcggg atatactttg tgaatgccag atagataagg taaacgatca gtgtataaat
     9781 catgatagct aaaacacctg tagggattcg gacaaatcgc tgaatgattt gaacaagatg
     9841 aatcatcaaa taagtaaaga taaatgtgag caaaatggtt gtaatcatac ttctaactaa
     9901 gaaaagaaca aaaacgacaa ataataaaac agtgagacgg cgtatttttt cattcgccaa
     9961 aaaacgctga taaacggtca aatcgacaac ctccaaataa caaaaaataa acacgttatt
    10021 cattatatga tggaatatga aaataagaaa gagcaaaaag aaaaaccgct aaatgacgtt
    10081 atcttatgag ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg
    10141 gtcttgatga aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc
    10201 ctgttcaagt ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa
    10261 cgcagcacct tgagcagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg
    10321 acgattaata taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc
    10381 cagacacaaa tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa
    10441 catcacaaga tactgggtat aggtaagatt cagcggcttg aggaggggag tgtagtgtct
    10501 aatgatttct ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc
    10561 ttccattttt ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa
    10621 gtctattcac taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac
    10681 ggacacactt tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg
    10741 atagatcctt caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa
    10801 acttgtgcac tgacagtaga agccccagag atattttttt gctgtttgat caattctaat
    10861 gcactgttaa aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt
    10921 tcgacgcgtt taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgcact
    10981 tctccagagc gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaaatta
    11041 ccacctttaa ttatatttta tacaattgaa ttttacacaa tataattgtg gaaagcaaag
    11101 aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat
    11161 agcaaacata tacaaaaaat atcagagaca aaaaataatc tcatcataaa cattacatat
    11221 atttctcaaa aagttgtttt ctttcttcaa aaattacttc acgatggacc tgatgaagca
    11281 tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt
    11341 ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga
    11401 tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca
    11461 aaccaagaga cgtgttttca tccagcatat ggaatctcgt accatacaag gtaaaaatga
    11521 cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg
    11581 tttgatacag ttctagtgct ttcaaactat catttaatac aaaatcgatt tccgttcctt
    11641 tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc
    11701 agctaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat
    11761 gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg
    11821 gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca
    11881 ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac
    11941 gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca
    12001 taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg
    12061 caatagataa aatagcataa agaaactctt ctattccaaa aataaacaga ttaagagaaa
    12121 caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa
    12181 aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa
    12241 ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca
    12301 ttgtttctgg aactattgca atgattaaag gaaaaaccaa gcttccgaaa agtactttaa
    12361 aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta
    12421 aaataactat agctcggtca aagcctaaag cagcttccag taatattcct agaccgctga
    12481 cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga
    12541 agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatt
    12601 cacttcctta attcattaat aatactctta tcttattata acttactttt caccttaagt
    12661 taactttaat taataaaaaa attaggctag tctaaattat tatgtttcat tcttctagaa
    12721 tatacttcta cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat
    12781 cgcttaagat aaattcagga cactaaatag tacaggaagt agactaaaac ttttaaaagt
    12841 tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac
    12901 cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg atgacgtcat
    12961 attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg
    13021 aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg
    13081 atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact gaaaaatggt
    13141 atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa
    13201 ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt ggggaagata
    13261 acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg
    13321 aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag
    13381 aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca
    13441 aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact
    13501 ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg gaccttggca
    13561 tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga
    13621 ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg
    13681 atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg
    13741 tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact
    13801 ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg
    13861 ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag
    13921 caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg
    13981 atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat
    14041 tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt
    14101 cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcatttacag
    14161 attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa
    14221 tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag
    14281 attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt
    14341 ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa
    14401 tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg
    14461 aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt
    14521 ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt gaagataagc
    14581 tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag
    14641 aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag
    14701 aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac
    14761 cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa
    14821 cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg
    14881 tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc
    14941 ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata
    15001 taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa
    15061 gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg
    15121 aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg
    15181 acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca
    15241 agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta
    15301 ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc
    15361 caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa
    15421 atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg
    15481 tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat
    15541 ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag
    15601 tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg
    15661 tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc
    15721 tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag
    15781 caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga
    15841 aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga
    15901 aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc
    15961 tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat
    16021 gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac
    16081 aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa
    16141 aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct
    16201 tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg
    16261 cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca
    16321 gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc
    16381 gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact
    16441 ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa
    16501 ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga
    16561 gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat
    16621 aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt
    16681 tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc
    16741 aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc
    16801 ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg
    16861 actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac
    16921 cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc
    16981 ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc
    17041 aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct
    17101 tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga
    17161 tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat
    17221 gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct
    17281 gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat
    17341 ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg aggagctggt
    17401 tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat
    17461 tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg
    17521 gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga
    17581 gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt
    17641 cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata
    17701 ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat
    17761 tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa
    17821 tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta
    17881 tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag
    17941 cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag tactggttct
    18001 tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact
    18061 ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca
    18121 ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat
    18181 gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg
    18241 aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag
    18301 tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct
    18361 ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga
    18421 gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt
    18481 ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta tttttagttt
    18541 ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg attccattag
    18601 aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac
    18661 ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa
    18721 ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt
    18781 ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga
    18841 tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat
    18901 ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa
    18961 gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct
    19021 tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt
    19081 tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact
    19141 ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg
    19201 tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg
    19261 cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat
    19321 taaacttctt agttgattct tttctttttt agtattaacg taatagggta gtttttttaa
    19381 ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt tctttctcgc
    19441 tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat acggacactc
    19501 ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt acaagtaacg
    19561 catctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag ggaagtatcc
    19621 aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga aaagattatc
    19681 taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg
    19741 cgcattatga cttaggtaac gacttttata aactttggct agatccaact ttgacttatt
    19801 cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc aataaagtcc
    19861 atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat atcggttgtg
    19921 gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca actgggatca
    19981 cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa catttagaag
    20041 ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg tttgatcata
    20101 tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag
    20161 tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc agccgacaac
    20221 aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt tatgtccctg
    20281 gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt gatttggaaa
    20341 gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt catcaagtcc
    20401 aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat ctatatttac
    20461 aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat ttgcttgttc
    20521 aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa
    20581 agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt tttgtctaac
    20641 ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa taaaagtttg
    20701 gttctttaat ttttaaacat gacagaaaat tttttcttcg ttatacttgt aatagaatac
    20761 tattttataa caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt
    20821 gcagctttgt ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg
    20881 tcttccagta gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca
    20941 tcttcgtcta gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg
    21001 tctgcagctc gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt
    21061 ttggaattag atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa
    21121 aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa
    21181 actctagatc gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat
    21241 aaattgatca cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg
    21301 gcaacagatt gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa
    21361 gacggcaatc aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact
    21421 gaacaagatg attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat
    21481 acactagaca aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg
    21541 ccaatcatat ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc
    21601 aggaattaga tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga
    21661 tcaatgaacg aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca
    21721 attctatggg acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg
    21781 aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc
    21841 aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat
    21901 taaatttatc ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca
    21961 cttatgaaac actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc
    22021 ctccttattt tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat
    22081 tgacctatgt caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag
    22141 aagcagtagt catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc
    22201 cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca
    22261 ttggtacagg tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa
    22321 gcatgttaca gattggtacc gagcttcata aagaagggcc ggaaattttt tctagaatca
    22381 taaaagaatt aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag
    22441 gcaaattacg caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt
    22501 ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa
    22561 cttgtgggaa aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc
    22621 aaaagagctt aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa
    22681 aataaagaag caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa
    22741 ttcccacgag ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc
    22801 tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt
    22861 tgaaacacac cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct
    22921 agttcctttt ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt
    22981 catctatggc ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg
    23041 aggagctgct gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg
    23101 tggttctatt agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg
    23161 gacgatgcca gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg
    23221 cttgacagtt tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc
    23281 cggtgcgcca tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt
    23341 gaagatagct ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact
    23401 tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc
    23461 ttacccacta gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac
    23521 agcagcaatg atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact
    23581 tgaacccatt tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat
    23641 cgattctctg catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata
    23701 cgatctattc ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca
    23761 aagtgatgcg ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt
    23821 agacgttgtt tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc
    23881 taatctgact ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc
    23941 tctaggaatc caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta
    24001 tctatttgaa gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg
    24061 agaagaagaa tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta
    24121 agcgcttgcg gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa
    24181 aagaaagaat tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat
    24241 caaaatgaat tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat
    24301 gtcaaaatct cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga
    24361 gaatcagatt ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc
    24421 ctttcagaga ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca
    24481 ggaatccact cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc
    24541 tatatgcgtt tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat
    24601 gatcagtatt taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat
    24661 ctgcagaaaa tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt
    24721 tatttcaatg aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag
    24781 aaaatcgatg gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa
    24841 aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt
    24901 gagtgatgta ctttccccta attgattttt ccctcatgtg gacttcactt cctttcgtac
    24961 tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatttgg
    25021 tagggttgct tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc
    25081 gtttttattt atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt
    25141 tcgggttacc ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca
    25201 gcagtgcatt tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt
    25261 gggacgctgg tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc
    25321 cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag
    25381 gtacttcgtt agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag
    25441 gcggtcggac attcgattat ttttctctct atattttagt tgcactgatt tattgggtca
    25501 tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa acgttctatc catcagtcaa
    25561 tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta
    25621 tattctgcct agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga
    25681 ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg
    25741 catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt
    25801 agatacgctt agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct
    25861 gaaagtgaca aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa
    25921 ttattttgtt ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat
    25981 aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt
    26041 tctgattgcg aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa
    26101 ccgcttcatg gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt
    26161 aaaatatttt ccaaaagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga
    26221 tccattttct aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat
    26281 caatgagttg caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca
    26341 acagcagatc cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat
    26401 ttaagaggaa aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc
    26461 tccaataaaa tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg
    26521 atcgaattat ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc ccaagtagga
    26581 caattcaaat tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc
    26641 gtccctgcac agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg
    26701 tttcttgttt tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca
    26761 ggagttgatt cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa
    26821 aacaaaattg tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat
    26881 aaaacagatg acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga
    26941 ttttctgatt tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg
    27001 cttgctatcc atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc
    27061 tctgacagta acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa
    27121 atgaaaaaat ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa
    27181 ttattggctc tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca
    27241 cttatgcatc atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa
    27301 tattattcaa aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa
    27361 aaaggaatag aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga
    27421 agaagtggta gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca
    27481 atggcaaaac gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga
    27541 aagctatgta ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaggcgt
    27601 ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc
    27661 gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt
    27721 agaacatttg ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta
    27781 tccagctaat gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat
    27841 gattgaattt ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact
    27901 ataaaatcat ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat
    27961 agccaagagg ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat
    28021 gcttctcaaa tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc
    28081 ccttcatcga gaggtcgtga catagctttt gtcacgatct ctcggcttat cctacgagta
    28141 ttattcgata catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg
    28201 ctccatcgtt tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta
    28261 aatcttctac ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt
    28321 tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc
    28381 cgaaatactc atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta
    28441 ctgtcagttt ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt
    28501 cccactctgc aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat
    28561 aataatcctc ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct
    28621 tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga
    28681 taaatacata gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc
    28741 tttggaaaat atttccaaag cttttaaaag taactttgct tttttgtatt ttataccagc
    28801 aagccagtca caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat
    28861 ccgtgttttg cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg
    28921 atcccgatag aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta
    28981 cgctcagtaa aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta
    29041 accagtttag tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg
    29101 atgaaggcaa gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc
    29161 ccgtggaaga tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca
    29221 accgtgatcg ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc
    29281 tgttctttgt tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt
    29341 acaattgaag ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat
    29401 ttagaatcga tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc
    29461 gatacactca tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa
    29521 ataaaaactt ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg
    29581 cccatcccgt tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc
    29641 ttgatatagc gtagaatacc gataatagca gaaataaata caattggcat caatacggaa
    29701 agaaagaaag gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc
    29761 ccagcaaaag caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca
    29821 aaagttgtac gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt
    29881 ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca
    29941 aataaaccaa taatacctaa taaataactc aaataatcac tcctattcca aattccgttt
    30001 tattttacct gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta
    30061 gagtgaaacg cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa
    30121 gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat cgattaagct aatagtttcg
    30181 ttttcaacat ttataagtaa actttttcta ttggtaatat agattttcgg aagggtagac
    30241 aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt tgttcatcat ttttattgac
    30301 catcacagta gagtgggctt gtgctccctc aagttcagct agtttatcat aagcaacttg
    30361 agcgattgga ttcgttactg cactgattga taaagcgatc aagacttcat tgatgcttag
    30421 tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct tgtattgttt caagaactaa
    30481 tggtgacaat aaaggaatgt catctgcgat atgggctaaa tgttttactg catttaagat
    30541 aactgccgct gacgcatcca tcaatgaact tgtacgcccg gtaatgatga cttcatctgg
    30601 taattcgatt gcaatcacag cagaaggatc ttttgtagga ctttgcgctt gaatcttatc
    30661 tgcatagtca tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc
    30721 cataatcagt ttgattcgat taactgcttc ttcgtcgatc aatccttttt tgaagtcgca
    30781 ttctgtttgg aaataacgac ggatgatttc ttgtttagag gcttcttgga caacggtatc
    30841 atccacgatc ccaaatccta cacgattcac acccatgtca gtaggagatt gataaacaga
    30901 ttctttacca gtaatttttt caataatacg tttgattaca ggaaatgtct caacatcccg
    30961 attatagttc acagctactt tattgtaggc atcaaagtgg aaggaatcga tcatattgac
    31021 atcttttaga tcgactgtgg ctgcttcata agcaatgttc agcggatgtt tcaacggaac
    31081 attccatact gggaaagttt cgaacttgga gtaaccagct gcatgtcctt tttggctttc
    31141 atgatacagt tggttcaagc aagtagcgag ctttccactt cctgggccag gagctgtcac
    31201 tacaacgatt ggtttagttg tttcaatata ctcatttttt ccaaaaccat cttctgagac
    31261 gattttttct acgttgactg gatagtcttc gatttctgag tgtttataga ccttgatgtt
    31321 tcgtctttct agtttattga taaagacttt cgtggcaggc tgtccactat agcgagtgat
    31381 aacgacactg ttgattaata agccataacc acgtaattca tcgattaacc gcaagatatc
    31441 catatcataa gtaatcccat aatcaccacg gatcttatta cgttcaatat ctccagcata
    31501 gacgcaaatc agtatttcag cttgatcttt caatttttgc agcaatttga ttttcgcatc
    31561 ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg tcaactaact tcccaccaaa
    31621 ttctaaatat aatttgtcat agtggttcac tcgttccaaa atatacgctg actgctcttc
    31681 aatatatttt tctgaatcaa aaccaatttt tttcactagg ttccctcctt aagtactaaa
    31741 gcattttttc atttttttga ggattcgtca actggaagag cgaaattcct caaaaaaaca
    31801 aagaaaatgg tttaaaccaa cttgtctaga tagttattca ttggtatgat agtttttgat
    31861 aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa cactatatcc
    31921 gttcgatttt taccggttat gaaagaatgc ttgaagatcc cgaattggaa caattgacgt
    31981 ttacttatct aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc attcatttca
    32041 caacaagaga tctgcctagt aaataaaaaa taagataaag gtcatcggat tcgattctgt
    32101 cctatcagat ggaaagacta gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc
    32161 tgttttatgg agcattgccc gctctttttt ataacaattt tttacagaaa atggtactat
    32221 tgaagtatta ataagtgaaa aacagatgag taagaggagg caagtagtat gaaagagcca
    32281 gatacaaatg caaattatga gccagacgaa ctagaggaga cgaacgcgga tcaggataca
    32341 ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa tctttcccaa
    32401 ttattagaag acttacaggc ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc
    32461 taccgtttat acaatggccg attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac
    32521 gagatcgatc aattgctgat gcgtaagatc catgatcgtt ttttacagga atttcctttt
    32581 atggaacaag tagaaaacat ctcgccaacg atgagctatt acaaaatcgg cacttattac
    32641 catgatcggc ccacggtcgg tattgatgca agtctgccag aaatttttgt tttgcctaag
    32701 atcgatgaag agtgggagaa atattcccaa ccggagtcaa cggactatga caaaaaaatc
    32761 aatgagctag acgcaaaggt gatcactgct cagacagaaa ttgaacgttt agatgaacaa
    32821 ataaaagaaa tcaatcgaca aatgactgat ttgaacgaca ataaaggatt cttgaatcgt
    32881 aaaaaaatcg aggaagaaat tcaagaatta gagaagaaga agcaagtact atctaatgaa
    32941 aaattaggct ggcttcctta tattgagacg cctgaaacaa tccagcagca aaaagaagca
    33001 ttgaagcaag aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg
    33061 caaatcagac gctatttcgg tagcaaagag ggattcggtc aagcaatcca tgaattcttg
    33121 atgaattatc ttggcaatga gaatcctaat aatcaatcta atgaaggagg ttccgagtat
    33181 gaatgaacac gatgaagcag aatggacaaa aaatgaagaa caaacagaag atcatgaaga
    33241 aacgaacact tcattggaag aacaaacaag taatcctctg gatgacacag aagaaatccc
    33301 tatgctagag gaaacaaaaa tacatgaaaa agaaaaagta actccgacga atacgaatac
    33361 tgcagaaaac acccatgctt acgaaataac tgaaaatccc gggtatttag aaggaaaaga
    33421 gcaagatcat caagatatcg tcagtttgat tgaaacagcg gaagcagaat tagcggattt
    33481 acgtatgaga aaagatttga tcgaatcaga ttttccacaa ttattagaat catatgatcg
    33541 ttttatattg aatgaagaac caatctccac gatcggaaag acaagcctga tcgaatactt
    33601 cacgtatgaa ttactgaaac cgttgaatga tattggtctg gaacggtcaa attcagctta
    33661 tgacacatgg aaaacaagac atttttcgat caattatacg ttgaatggac aaaacgaatt
    33721 agtcttttcc tttgtattac cgaccatcga tcaacgatac caagtggaat cgatgagtct
    33781 cctaaacgta tcaccagaga caatggaaat caatgtccaa gatgaccggg tgctttcttt
    33841 gatccgacac tggtcagtgg atcgagtatt ctctgcaggg cagatcacga tattcaatca
    33901 caaattgaat caattattag cccatgcaag aacacttggt tttaccgtta atcagacatt
    33961 actagacaat acgaagccac ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga
    34021 agtattggat gatattttta tcgtggcaat gaaaaatcca tcctacgata tggaaaagat
    34081 caacgaaact acttatcaag tacgattaga taaaggacag tctttaacga taacaacaaa
    34141 cgaaaaagat caaacagtct tgtcgatctc atctggagac tacccacgat ctgtcattga
    34201 tttctttatc aattacgaat tcttggtccc attaatggtg agaaagtcct gattggataa
    34261 gttggattac cagatgtcat tcagataatt ctgttccaaa ggacttgcgg cagttatcca
    34321 gaataattgt cacaagtctt ttctgtatgt actaaatgga cgtgaataac ttgctgatga
    34381 aggagggatt tcattaggaa tgtccacaaa taaaatagca cagcttagct tattgtctgc
    34441 tgcttgtgta gcaggtagga tcgcttttca atttattccg aatttccagc caatgactgc
    34501 tattttttta tttatagtac tttacttaag cttaaaagat gcgttaattg tcatgagtct
    34561 ttctattgcg atcagcagtt tttattttgg tgttggacca tgggtcatcg gccaatggat
    34621 cagctatact gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag
    34681 gaatctatgg ttaaagggag tctgtttttt tctggcgggc atggtttacg gaataggtat
    34741 gaccgttttt gatacgctgt tatatcgttt gcctcaacct tggatctatt accttcaagg
    34801 ggtgtcgttt gatttgatgc acagtatagg caatgttgtg ttctttcttg tttttctgcc
    34861 agtcgtcaaa cgtttttaca atcattcagg aggaaaaaat gaaaaaaata atttatagtt
    34921 tgcttgcaat gagtgtcagt atactcattt tgacaagttg cagtacaacc tatacacaat
    34981 cggcttccaa ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag
    35041 aaaatggtca aaacttttcg gaaaaagaag tggtcttcaa aaaaggcgat gatttactga
    35101 gtattttgaa acgaaatttt gaagtcaaag aagataacgg cttcatcact tcgcttgaag
    35161 ggcactcaca ggatgagaaa aatcaactct attggatgtt tactgtggac gggaaaagtg
    35221 ctactactgg tgccaaagag atcaaattga atgatggaca atcggtcatt ttcaatttgt
    35281 ctaaactata agtttcataa aaaactgtta aagggcgaat attgccataa ttcagtgagt
    35341 tcagttgatg aaagaaggaa aataacatgg aaaattttaa aaaaagcgga atgttttccg
    35401 taattgctgc tctaggggct aatattctag ttgctatcag caaatttgtc ggctatgcaa
    35461 tcagtggcag tgccgccatg ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc
    35521 agattttgct gttattcggt gataaacaag ccaataaagg acagagcgag ttgcatcaat
    35581 ttggtgaagg acgagcaaaa tactttttca gtacaatcgt cgcaatgatg cttttcttcg
    35641 gcggtggggc acttggggtg atggaagctt ttgaaaagtt gacgcatcca tcccatgaag
    35701 tgggtaatcc gatcattgtt atagcaatac tgttatttgg tttggtaatc gaaggaagtt
    35761 cgttacgcgt ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt tccacgatgc
    35821 gctttttgcg cgaaagtcga cacagtgaaa tactgattat ttttacggaa gacttgtgtg
    35881 ctgtaatcgg tttggtcttg gctttagcag gaacaatatt gacttatgca accgggattg
    35941 ctgcatttga tgcgatcagc ggattgctga tcggtttctt gctgatggga gccgccattt
    36001 tcctagcaaa agaattttat agtctgatca tcggtgaaag cgtgacagca agcgatttat
    36061 caaaaatcaa gttagctttt gaacgtcctg agatcagtcg gctgatcgat ataaaaacag
    36121 ttcatttaag tccaacggag attttagttg cagcaaaagc agatatcgtt ggtaacaaag
    36181 agaatgaagc ttactcggtc atcaatgata ttgaaaaaaa tatgcgtcag tctcttcctg
    36241 acaagaagat gtatatatac attgaaacag ataaatatga cccaaattat tctcgtaaat
    36301 aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa
    36361 aacggctaca atttagcgaa tttatgttac aatgagtccg ctgtttattt gttgtaatta
    36421 aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag
    36481 tgatgccact ttgtataagc tatataccag taggattagc ttgcggagta ttgttgcaaa
    36541 aagttggatt tgatcctctc ttatcaggac tgttatcgat tctggtcttt tccggtggcg
    36601 cgcagttttt agttgcatct atgcttacga cgcaagcatc atttgcaacg acattattga
    36661 tggttttctt tttagaatta cgctatacat tattaggagc aagtcttgcc ggttttatga
    36721 aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg
    36781 cggtgaacta cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg
    36841 tcaattggtt ctcaatgact gcatgggccg gtagcaatat gatcggtaat tttttcggct
    36901 cggtgattcg agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca
    36961 tgtttatcat gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat
    37021 taggggtcgt attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca
    37081 ttgcttcatt tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac
    37141 taagagaccg cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca
    37201 atcaccatga ctaaactata tttgctactt ttagttgctt gtctgttcat cgtggcatat
    37261 atcccgcgct tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt
    37321 gactggatga aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc
    37381 attactcatg aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta
    37441 gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct
    37501 gtctttttgc tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat
    37561 aagttgttca aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt
    37621 tattcgtagg gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag
    37681 gaattagaga gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt
    37741 tatgaaagaa tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg
    37801 ctaacctttg ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta
    37861 agtatgatgg ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat
    37921 cttccttatg gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa
    37981 aaagtagctg tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt
    38041 acgatcaata tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct
    38101 cctttactag ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga
    38161 agtttatgcc catctttgat tgctatccca attcttagtg caatgagagg atatttccaa
    38221 ggaaaaaatg atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg
    38281 atcgttatct tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa
    38341 gctgtattga tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg
    38401 tatatcgtcg gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt
    38461 tatttcgaaa gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt
    38521 atttttgtcg gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca
    38581 ctgcttcact ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt
    38641 gcttctgtca atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct
    38701 ataagcagct tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa
    38761 acagtaggag atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg
    38821 tctttattag ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga
    38881 tatttccaaa tggctttgct agcttctttg tttttctctt tattcacgat cctatctacg
    38941 atgcttcaat ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc
    39001 ctgaaagtca tcttccaagt aatcggatta ggattagttg gcggatacgg gatgagctta
    39061 tcaaatggct tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa
    39121 taccgaatag cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc
    39181 ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg
    39241 gaatcaaaaa gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta
    39301 tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat
    39361 agcagaagca aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact
    39421 cgtatgatta cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact
    39481 tatgatatag ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa
    39541 cttgcagtag ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac
    39601 ttattcgtta ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat
    39661 atcgaagcgg atgaatatta tggactttta aaaaacagta aagattttcc tactacttca
    39721 caacctgtcg taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac
    39781 acgattttaa gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg
    39841 atgaaagatg acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg
    39901 ccgatgggac acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat
    39961 gaaatacttg cacatatcga tcgaatccga gataatacct atgcttatct gattgtagat
    40021 gatttgaaca atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc
    40081 ttattaaaaa tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa
    40141 atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac
    40201 gaaatacaac cgccagtcaa actttacgtg atacatgcaa ataatttaga agtagcgatt
    40261 gcggagaaag agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc
    40321 ggaccagtca ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa
    40381 taattccttt tattcaaaaa acataccaaa aata
//
LOCUS       DAZUKZ010000016        38383 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000016 DAZUKZ010000000
VERSION     DAZUKZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.016"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            420..722
                     /locus_tag="ACG8EF_000787"
     CDS             420..722
                     /locus_tag="ACG8EF_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4946134.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            794..2020
                     /locus_tag="ACG8EF_000788"
     CDS             794..2020
                     /locus_tag="ACG8EF_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4946135.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(2108..2500)
                     /gene="rpsI"
                     /locus_tag="ACG8EF_000789"
     CDS             complement(2108..2500)
                     /gene="rpsI"
                     /locus_tag="ACG8EF_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS4946136.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2516..2944)
                     /gene="rplM"
                     /locus_tag="ACG8EF_000790"
     CDS             complement(2516..2944)
                     /gene="rplM"
                     /locus_tag="ACG8EF_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFS4946137.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(3478..3945)
                     /locus_tag="ACG8EF_000791"
     CDS             complement(3478..3945)
                     /locus_tag="ACG8EF_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS4946138.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(4065..5603)
                     /locus_tag="ACG8EF_000792"
     CDS             complement(4065..5603)
                     /locus_tag="ACG8EF_000792"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFS4946139.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5683..6369
                     /locus_tag="ACG8EF_000793"
     CDS             5683..6369
                     /locus_tag="ACG8EF_000793"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFS4946140.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(6570..7097)
                     /gene="msrA"
                     /locus_tag="ACG8EF_000794"
     CDS             complement(6570..7097)
                     /gene="msrA"
                     /locus_tag="ACG8EF_000794"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4946141.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(7181..7858)
                     /gene="pgmB"
                     /locus_tag="ACG8EF_000795"
     CDS             complement(7181..7858)
                     /gene="pgmB"
                     /locus_tag="ACG8EF_000795"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS4946142.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7855..8985)
                     /locus_tag="ACG8EF_000796"
     CDS             complement(7855..8985)
                     /locus_tag="ACG8EF_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS4946143.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(8985..11168)
                     /locus_tag="ACG8EF_000797"
     CDS             complement(8985..11168)
                     /locus_tag="ACG8EF_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFS4946144.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(11165..12190)
                     /locus_tag="ACG8EF_000798"
     CDS             complement(11165..12190)
                     /locus_tag="ACG8EF_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4946145.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(12235..13062)
                     /locus_tag="ACG8EF_000799"
     CDS             complement(12235..13062)
                     /locus_tag="ACG8EF_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4946146.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(13059..14126)
                     /locus_tag="ACG8EF_000800"
     CDS             complement(13059..14126)
                     /locus_tag="ACG8EF_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFS4946147.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(14152..14988)
                     /locus_tag="ACG8EF_000801"
     CDS             complement(14152..14988)
                     /locus_tag="ACG8EF_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4946148.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(14985..15854)
                     /locus_tag="ACG8EF_000802"
     CDS             complement(14985..15854)
                     /locus_tag="ACG8EF_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4946149.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15924..17207)
                     /locus_tag="ACG8EF_000803"
     CDS             complement(15924..17207)
                     /locus_tag="ACG8EF_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4946150.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(17221..18906)
                     /locus_tag="ACG8EF_000804"
     CDS             complement(17221..18906)
                     /locus_tag="ACG8EF_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFS4946151.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            19181..20206
                     /locus_tag="ACG8EF_000805"
     CDS             19181..20206
                     /locus_tag="ACG8EF_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4946152.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20801..24454)
                     /gene="rpoC"
                     /locus_tag="ACG8EF_000806"
     CDS             complement(20801..24454)
                     /gene="rpoC"
                     /locus_tag="ACG8EF_000806"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFS4946153.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(24533..28150)
                     /gene="rpoB"
                     /locus_tag="ACG8EF_000807"
     CDS             complement(24533..28150)
                     /gene="rpoB"
                     /locus_tag="ACG8EF_000807"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS4946154.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            28878..29846
                     /locus_tag="ACG8EF_000808"
     CDS             28878..29846
                     /locus_tag="ACG8EF_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4946155.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            29864..30769
                     /locus_tag="ACG8EF_000809"
     CDS             29864..30769
                     /locus_tag="ACG8EF_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4946156.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            30766..31572
                     /locus_tag="ACG8EF_000810"
     CDS             30766..31572
                     /locus_tag="ACG8EF_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4946157.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            31729..32697
                     /locus_tag="ACG8EF_000811"
     CDS             31729..32697
                     /locus_tag="ACG8EF_000811"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFS4946158.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(33014..33538)
                     /locus_tag="ACG8EF_000812"
     CDS             complement(33014..33538)
                     /locus_tag="ACG8EF_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFS4946159.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            33825..35636
                     /gene="scm"
                     /locus_tag="ACG8EF_000813"
     CDS             33825..35636
                     /gene="scm"
                     /locus_tag="ACG8EF_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS4946160.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(35791..36777)
                     /locus_tag="ACG8EF_000814"
     CDS             complement(35791..36777)
                     /locus_tag="ACG8EF_000814"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS4946161.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(36800..37366)
                     /locus_tag="ACG8EF_000815"
     CDS             complement(36800..37366)
                     /locus_tag="ACG8EF_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFS4946162.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(37380..37796)
                     /locus_tag="ACG8EF_000816"
     CDS             complement(37380..37796)
                     /locus_tag="ACG8EF_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFS4946163.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
BASE COUNT        11072 a         8127 c         7171 g        12013 t
ORIGIN      
        1 tgaaactacg cgtatagtaa gtagctttca aaatatataa aaaaggagcg ttctcaaatg
       61 agaacatgct cctaaaacta ctttattggc gtatggaaaa attatacttg aagtggatag
      121 cgggttgaat aagttgataa gcatcaaaac atttataaaa attattcaag actatgtctg
      181 ttccatatac aaatatgaaa atcagtaaac tccatttaac ttttttcgta gtgtgtcttt
      241 ttatacatgt ttatcgttat taatagtgaa taggaaaaaa actatttttg atcattgaca
      301 attaaataca atttttgaat aacgatccct gctattcgag ctagaaactc aagtacgcca
      361 tgacctgatt agcgacaaat acttagagat ttctttttgt tctgggaaaa caaaatgcca
      421 tgacaatagc agcaaataat gatacttccg taaggctcgc cgtaaactgg ggagaggtga
      481 aattcctatg tgcttaccca aagcaattca aaaatttcat aactactatt ttaggagatg
      541 attttataga atccagagtc tacctgaata aaaaacaaac ctgtgcattt ctaggaatcg
      601 ataataatac tctagataag tggattcgta tatataattt cccagtaata agagttggtg
      661 gtgtagttcg ctttgacgta gaagatatta aagactttat gaatacattt aaaataactt
      721 gaaattttct tctcgccgca tatactaaaa ttagtgaaat atactgcctt acttttttta
      781 aggagtgagg attttgccta caaagctttc aaatgggaaa tataaaacta atctgagata
      841 tccaaaaagg tttaaagaaa taacaggaat tgcatcagaa aagtatcaaa aaacatttcc
      901 aaataggcaa ttggcgatta aagccgaaaa tgatatgaag aagaaaatag aaaaagtttt
      961 gagagaggag aatgcaaact cattagaatt aaaaggaaaa ataacgttta aaaaatttta
     1021 cgaatctaaa tggcttccta gatatgaatt aggtcaaaca attagatcaa acaggcctcc
     1081 tagtgatata accattagta atacaaaaga tatatttcgg ttgcatattt tacctatgtt
     1141 tggtgaatac gcaatgaatt atctaaatat caatactgag ataatatcag atgaattaac
     1201 caaaaaatcg aaagaatatg caaatataaa aatcataaaa ggatatgttc gttccatgtt
     1261 cgatattgca gaaatactaa attatatcga atttaatcga actactaaaa ttattcaaag
     1321 tatcactgca cctaaaaaaa atgctttaga agaaaaaaga atacaagaag gtaagcaagc
     1381 actatcatca aaagaactta ctaattggat agaagcagta aatgatgatt ttaataatca
     1441 tttacttact tttcatgatt acacactttt tatgattacc ctgtatcttg gtgatagaaa
     1501 aagtgagact tacgctttgc aatggaaata tattgatttt gaaaaacaaa cggtccgtct
     1561 taaacatact ttagataaat atcaaagaaa aaagtttaca aaaggtcgaa aagataccgt
     1621 cattcaagta ccagaagttg taatgacatt gctaagtgaa tggaaatcgg tacaagctga
     1681 tcaattgttg aaattaaaaa ttaagcaaac gttagatcag tatctcttta cttatactaa
     1741 accatctgga gaagtaaatt gtcctgttca tgccgattac ctaaactatc gaatcaattc
     1801 aattaaaaga aggcatcctg acttagttca tttatctcca cacaaactcc gtcatactta
     1861 tgcaactata gctagacaag gtggagcaga tatgaatcaa atttcaaatg ctttaacaca
     1921 ctcagatatt tctacaacaa agatatatgt gaacacacca gatatagttg ataaggctgt
     1981 ttttgaagct tttcagagag gattgaataa gtgtgattga tatgtgttct taaatgtgtt
     2041 cttaaaaaca aaagaagcac tttccaaata attgaaaagt gccagaaacg ttgatatata
     2101 aaggatatta acgttttgag aattgtgaag ctttacgtgc tttcttaaga cctggttttt
     2161 tacgttcgac catacgtgcg tcacgagtaa gtaagccagc acgtttaaga gctaaacgga
     2221 agtcaggatc aacttctagc aatgcacgag cgataccatg acggatagct cctgattgtc
     2281 cagcgtatcc tccgccgttt acgttaacaa atacgtcata tgcgcctttt gtttcagtaa
     2341 cagcgaaagg ttgattgatt acttcacgca agtcagcatg tggaatgtat tcttcaacat
     2401 cttttttatt tacagtaatt ttgccagttc ctggtactag acgtacgcga gcaactgcgt
     2461 ttttacgacg gcctgtgccg atatattgaa cttgtgccaa tgaaattccc tcctattaga
     2521 ttaagtttgt gatgtctaaa acttctggtt gttgtgctgc atgtggatgt tctgctccac
     2581 cgtatacgtt caattttgta aattgtttgc ggcctaaagt gtttttagga agcatacctt
     2641 ttacagaagt ttcgatcaaa cgacgagaat ttttagcacg caattcgcca gcagtgattg
     2701 atttcaatcc tcctgggtag ttgctgtgac ggtaataaat tttgtcagtc gcttttttac
     2761 ctgttaattt cactttgtct gcattgatga cgatgacgaa atcgccagta tccaaatgag
     2821 gtgtgaaagt tggtttattt ttaccacgta gtacagatgc aacaactgtt gaaagacgtc
     2881 ccaatggaac atcagttgcg tctactacat accatttacg ttctacttcg cctggtttgg
     2941 ccatatatgt tgtacgcacg ttattttcct ccaatttaag ttctgatgtt tgacatacac
     3001 ttcgagtttc cggggctctt tgtggggcaa acaataccat ttaacatatt acccgctaat
     3061 ctagtcaatg tcaacgtttt tctgcttgat tgaaaaattt atatgaaact ttacttcttt
     3121 tctttctttt atataaaagg gtcgtttcct gtcaaaatga aaaacaaaaa gaggctgtga
     3181 caaaagtctt gtcacaaccc cttattttga ataaacggtg ttctcctgcc aactcctcgg
     3241 tcacaagtca caatatccat aaaacataag aagctgtttt ttaaaattgt ttcttgtgac
     3301 tcggagctga acggcaggct tacaacctct tggtaaacgg tgttcaatca gctgaccctc
     3361 ggtattagct caaaaaacgg caaaatatga ggaactattt tgacgtttgt ttctctaata
     3421 ctcaggtcaa aacgctgatt tcacaacctc tttttcggtc aagttcaaca acagttttta
     3481 cgcgtcgatc tctggcgctt tgcccaaatg agcttggatc atccagatac gtttttctgt
     3541 ggctgttttg aattcgataa agatatcttg tgttggatcg tcgccttctt ctccagaaac
     3601 ttcgattcct ttttggtaaa gatctgctag ataacgatag cctgcaacaa gtgtttccaa
     3661 acgttctgga atagtacgat cccagcggcc aatttcatca gggatttttg tgttatctgc
     3721 aaattcacgc aatgtagaat aaggtgaacc gtctaatgtg atcaagcgtt cggatacttc
     3781 atctaactga gcgttcaaat catccatgaa ctcatccatc attgggtgca atgtaaggaa
     3841 acctggtcct ctcatgtacc aatgtgtttg gtgaacaaca acagacattt ggcttagatc
     3901 tgcaactaat tgatttaata cttctttggt ttgttggaat ttcatctttc ttgcctccta
     3961 acattttctt acacttaaaa taatagtaga ctttcaatga aaagtacaac aaaatcattt
     4021 acctgagaac gattttcagt tcgctttttt atccgtaaag ccaatcattt ttcagaatca
     4081 gctaattctt ctcgcagttg agcaaattgc tgataagtct gtgctaaatg cttttgtgtc
     4141 gaaacaaata atggatagat tttctgatat tttttatgat cagtttcttg tactccaact
     4201 tgctctttca attctatcaa ttcctgtgct tcactgatac tttggatctt tccgatgctt
     4261 ctccagccta aaatcacagc tcctaatgcg gatgcttctg tgacagtagg gaaaacaagc
     4321 gggtaaccca gtacatcagc catcatctga cgaaaaatcg gagattgagc aaacccgcct
     4381 gtagcacgga tctctgtgag ctgattttcc tggcaaactc cttttaaaat ccgataaagg
     4441 ttgagacaga tcccttctag aatcgccttc atcatttctt tttctgtatg ttgccgctgt
     4501 aaaccgataa aactgccaaa cgcatctgcg tcccaaagcg gtgctcgctc accaagtaaa
     4561 tagggatgaa aaatcagacc attggcaccc gctggcacaa aagcgatagc cgaaagcagt
     4621 gcatcatatg gattttctcc tttttcttta gcagattgaa cgacttcctg taaaaatgta
     4681 tgccctgccc aatcaaagat gccagcacca ttgctagtcg ctcctcccac tacccaatgt
     4741 ttctgatcca gaacataaca aaacgtctcc atttctggat ggaactgagg ctgagtagta
     4801 ataaaacgaa gtgctccgct cgttcctacg gtcaaagcgg cgatcgtatc accaaaagca
     4861 cctaacccta gattagaaag cggcccgtct gcccctccca aaacaaatga tatatcattt
     4921 ggcaaaccta gttctgctgc tgtttttgat ggtagacctg ttacttgata ggtggaagga
     4981 aagacttgcg gcaattgctc tattcgaatg ttcaagtagt caagcacttc ttctgcccat
     5041 tggaattcat ggatatcaaa atatccagtt cctgaagctg ttccgtagtc gctgatcagt
     5101 tcgccaaaca atctataaaa gatatagtct ttgataccga caaaataagc agtttttttg
     5161 aataattcag gttggctgtt ttgcagccac ttgattttgt tcaatggtgt cattggatga
     5221 atcggcgtcc cagtctgccg ataaaattct ttgccaagca cggtatattt cagtttttct
     5281 gcttcgtccg ctgctcggtt atctgcccaa gtgatcattc gagataacgg tgttttctct
     5341 tcatccaaca atatcaggcc atgcatcgca gaggagaagg agacaaattg aatgattgtg
     5401 tctgaaggca actgtttaat tacttctttt ataacggtgc agacaccttg aaagatcaat
     5461 tcctgatctt gttctgccat cccttgtgtt tcttggatca aagggtagcg aaaataagcg
     5521 gatgctgctt cttgggcttc atctgtgaaa gcaactgctt tggtttgtgt ggttccgata
     5581 tctaatccga ttgaaacgtt cattttttca cctcaaattt tatgtatgta caaaaattta
     5641 gtttaagctt gcgtatcttg gttttgaaaa gggggagatg gcgtgttact ttattttatc
     5701 attggttgct gtatcggatc atttttatgt ttaaccgccc agcggattcc gttaggtcac
     5761 tctattattt atcctagatc tcattgtgtc aaatgctgtc gttcgctttc ttggtatgaa
     5821 ttgattccga ttctctcgat catcgtccaa cgatttcgct gccgctattg caggtgtcgg
     5881 ttgcccttct actatttgtt ggcagaagcc ttatgtggtg gtctttttgc ctggttcttt
     5941 actttttctt ccgcaagaaa tttttcgacg ttcatatgga tgctttcggc tttgctattt
     6001 tcgttgatgg atttatttta cttcatggtc cattctcaca cgctattctg ttcttgggca
     6061 attttatgga ttttttggtt gcaggccggc gtcttccaat ggcaaagtgt gctgttaacg
     6121 tttatcgtag gcggtgtgtg ccttcgctat ggtcaatcct atctcggagc tggcgatact
     6181 ctgttgatgc tttcttggag tggtggcttg tctctagaag agttgcttca gatcttgttt
     6241 cttgctagtc tgtttggaat atccgttttt tctgtttact ttatctgtca taaaaaaaag
     6301 ttggaaaagc tgcctttcat tccttttttg agcagtgcgt tgctgattgt ccttcatctt
     6361 aggtattgaa aaaaggttgt gacaaacgac ttgtcacaac ctctgatata tagtgttctc
     6421 ttaccaaatc cttgtgactt ggagctaggc gacaggttca caacctctcc actaaatggt
     6481 gttcaatcag ctgaccctca gtattagctc aaaatgctgg ttttacaacc tctgatatac
     6541 ggtgttccat aagtagcttt ttcggcttct tatttcttga cgccccaatg tcgacgaagg
     6601 aaacctgcac gtccagaacc ttctcgataa agattgtaat ggagcttgtt tttcttgtaa
     6661 taatcctgat ggtaatcttc tgcaggataa aattcgcttc ttggttcgat ttttgtcacg
     6721 attggcttat cgaatcgccc gctgtcttgc aatgcttgtt ttgaacgctc tgcaatattt
     6781 cgttcttctt catcattata gtagatcact ggacgataag aatcaccgcg atcagcgaac
     6841 tgtcctaatg cgtcagtcgg atcggtttgc tgccaataga tttccaccaa ctgatcataa
     6901 gaaataactt ctggatcata ttcgatctct actgcttctg tatgccctgt tgtgcctgtt
     6961 gttacttgtt cataagttgg atttggtaca tgcccgccag tataaccgga tgtcacagaa
     7021 atgatccctg gctgctcttc aaatggtttg accatacacc aaaagcatcc gcctgcgaaa
     7081 actgcttttt ttgtcatata aaatccccct tttttctcat cataccttga tttccaaaag
     7141 gaggataacg ttttgcttgt gacagaaaaa acggaaacta ttatatacgt gatacggctt
     7201 ctgataaatg ccaaaacgaa cgtacttgat caaaaacggt taaatcagga atcacatatt
     7261 cagcagaagg aagatttatg ttttcaccta ttcccaatac atgcatgtta ccagccaaag
     7321 ctgcttgaca accagcttct gaatcttcaa tgaccaagca tgcatttggc ggtacattca
     7381 attgctgtgc tcctttgaga aaaacttctg gatcaggttt tgcttttgat acttgcgtgc
     7441 cgtcaataag gacatcaaaa taacttgtca aattcgtttt ttctaaaatg aggcgtgcat
     7501 ttttgcttgc tgatcccaat ccgatcttga tgtttgtttt tttcaagtac tcaagaacag
     7561 ctattgcacc aggcaaaacg gatgtttcat ccattttttg aatcgcttga agatatagat
     7621 tgtttttttg ctcagcaaag gctgatttct ctttttctga gaatttctgt tccatttttc
     7681 caaatgacag caatcgctct agtgactcgg ttctggatat tcctttcagt tgctcgtttt
     7741 ctttctcagt gaatggaatg gacagctggt tagctaaaac cagccacgct tcgtaatgat
     7801 aacgagctgt gtctacaaga acgccatcta aatcgaatag tgctgctttg attttcatcg
     7861 attacctcct tgaaaatgaa cgacttgaag taaagatgcg atgtttttag ctgtgcgatg
     7921 aagattatca gtggcttgct gtaaagcttc ttgtaaaggc acagctgcag gaaggatcgg
     7981 aaaaacggcc gaaatgccaa catctgctgc tttttcgctt cctttaccaa cacttccgca
     8041 aatagctatc acaggaacgc cctctggtac tcttctagca atcccaactg gcgcttttcc
     8101 ttttagactt tgtccatcta gacgtccttc gcctacgatt acgaggtctg cttgctgtac
     8161 ccgtttatcg aactccagta aatccaatac gtagtcgatg ccagagacta ttttccctga
     8221 agcaaatgct aacagtcctg cacccattcc accaccagca ctactgcccg atgcctcgat
     8281 tacttgaggg gcaaatctca tataaaaatc atgcatggct tggtcagcca ctgctaattc
     8341 attttctttc aagccttttt gtggtccgaa aacaaaagaa gcgccttctt ttccgcataa
     8401 aagattagta acatcagtaa tgacagtgat tttggcttta atcagctcaa taggcacgtt
     8461 cgttttatca actgtagcaa ttttacctag attttctccc agtgcttcta ctttttgatt
     8521 atcttgatcg taaaaagcat aacctagacc ttctgccatg ccgatgccgc catcgttggt
     8581 actacttccg ccaacgccga tgatgatgtg tcgaacacct tgttgcaaca attgtcgtat
     8641 cagttctcct actcctcgcg tggataacct aagtggctgt tgttggttta gtgggacatc
     8701 tttcaatccg acgatttctg ccatttcaaa aatcgcagtt tgaccgtctg aagcgtactg
     8761 gatttctttg tttttgccaa atgcattagt aacgactgct gtttgccgct tcaaccttaa
     8821 actgtctgct aatgatgaaa ccgttccttc tcctccatcg cctataggta gcagatcata
     8881 ttcagcctga acaaaaactt cttgaaaacc tgattgaatg gcttgtgctg cctctaaggc
     8941 tgtcagactt tctttgaaag aatctggcgc aatcagaata cgcattattt tcccctccgt
     9001 tccaagatca gttctttttc cagctggtat tggtctccat tgacaaaaat aggtaatgtt
     9061 tctttcgtct ctttttttat tactatttgt ttttttgtca aaacaaatag caacctgtga
     9121 tttttccaaa taaaaggaaa agaaattttt tcccatgctg aaggaagtct tggagaaata
     9181 gaaagagttc cattcgttac tgtaatacct gaaaaaccaa atataactgc tagccatagt
     9241 gctcctaatg aagcagcatg aattccgtca tctgatgaat gcggatctgg tcctaaatca
     9301 atcaggcacg cttcttggaa taaatgatac gcccattcca tttcttggat acggcaagcc
     9361 tctatggcgt gaatcgcttt actcaacgat gagtcgtgga ttgttttgtt ttcatagtaa
     9421 tgcaaattct tagaaacgat tggttgagaa aaatactcag gtaataagta taacagcatg
     9481 acaagatctg cttgcttgct gacttggagt tcatttactt cgctacgggc ataatcaagt
     9541 aaaatagact ggctgccagg acgttctttg taagtggtca aatcaataac tggcttactg
     9601 agatacgtgt catcttgcgg aatcagctgt tctttgttag gcactggcaa atataactta
     9661 tctaaaaaat tttgacattc tttcaaaaaa agcggatcgc cagcttgata tttaacagct
     9721 aaggccacat tataatgcgc cagatagttc gtatatgcat tattgtcgat atattctgtg
     9781 tattcatctg gaccaatgac tctgtgtatc tctaaatgat catcgacttt tacagctctg
     9841 ctcagccaaa aagctgctgt ttcatgaatg agcgtttttc cctttgtttc taaaaacgat
     9901 tgatcctctg ttgcttggac atactcttga acggcataag caaggtctgc actgatatga
     9961 tgttctgcgg cagctgaagc aacttcttgt cttgttccgg ttttgatatt caatgcagca
    10021 aatttgggtg tctcttcctg accagtgtgt gcgctttccc atgggaaaag tgcaccttga
    10081 tagtgataat ctcttgcttt ttgccgtgcg ccttctaatc gatcataacg gtacagcagc
    10141 aaattctttg ctacttcagg aaactgatat aaaaagaaag gcaggataaa aatttccgta
    10201 tcccagaaaa catgcccttt ataaccttcg ccagttagtc ccttcgcccc tacactaaca
    10261 cttggatcat tgcggggagt cataatctgt aaatggtaag cagcgaagtc catcgctaac
    10321 tgatcgaaag aagaatcgcc ttggatcgtt atccgatgct gatgccaaaa atctgtccaa
    10381 taagacatcg actgctctaa aatttctgta taactaacgc ttttctgggc ttgggcaata
    10441 cttttcttaa acgggtctgt ttgctgaaga gaggtagaga gcacgatgat cttctccagc
    10501 gtaaaggacg tattttttct gatcttttct gaaaaggaag cggtgatccg gcgatttttt
    10561 gcttggaata cacctgcttt attgaattta gctgcaatcc caatatcaat tttactttct
    10621 gttgtctggt aggatgcact gatcaaatcg tcaccaaata ctcgcagttc tttttcaacc
    10681 aactgctgtg taccaaaatt ggtttgctgt ccatcgatac ccgttttcac atctacttgg
    10741 cagtcttcgg tcaacggctt gatgaccact ttcagggctg cctgatggag attattttga
    10801 tcagcaacac gacaagtaat gacttccact ttttttcctt gagcagtttc aatgagcgca
    10861 tagcgtttaa gctctcccat ttctaaatct agtgcccgct cgtatgcaag aactttcatc
    10921 gtctcaagag aaaaatgttc accgtcgatt tttatttccc atctgatgac atctgggaga
    10981 ttcactaact cggctgattc tgctcctaaa ggtcggttat atacaccagc tgcaaacatt
    11041 ccccttactt gattccgata gtcttcctcc tggcttcctc tgataccaaa aaatccattt
    11101 ccaatggtca tctgactcgc atacttattg atatactcta aatcaaactg tttttcttct
    11161 actttcataa ggtaataact tctcctgttt ctgccgaacg atataaattt tccaccagat
    11221 tctggatgac atatccttgc cagccatctg caatcatctg agtctggtca ccgagcgccc
    11281 gattcacaaa atgagccata cttagttcat gtgggtcatt aactggcgtg gtcttttgta
    11341 aggaaaccag ctttccatct acatcttcga atagttcgcc tggatacaga ttagcacctg
    11401 cctgatcgcc acaaaaagcc acattcagtt tgtcttctct gatattcaaa gcaaacgaag
    11461 tattcagttg gatgatcttg ccgttttcta attccagtgt gccaaaaatt gcatcttcta
    11521 ctgtgaattt gcttggatcc catttcccga atttccctga attttttttc gtaccaattt
    11581 tttggaagct gtacgcccga acgcttttta ctttaggaaa tccagtaata tacatcgctg
    11641 tatcaagcat gtgaatgcca taatcgatca aaggcccgcc tccttgaagt tctttgtcga
    11701 taaacgttcc ccagcctgga acaccagacc gacgaagcgc attcgcttct acgtaataaa
    11761 tatccccgag aacatcaaga tgattcttga tcattgttgc ttcgtcagaa aaacgatgct
    11821 ggaatccata agccaaaatc tgcccgtttt tatcggctgt ctctgccatc tttcttgctt
    11881 gatcagctgt catagctggt ggtttctcgc acatcactga agcattagct tcgagggcag
    11941 ccaatacatg ttcgaagtga aagcgattgg gtgtacagac actcacgata tcgggagaaa
    12001 tctcattcag catttgtgta actgatgtat acgcatgaga aaatcctgta cgtcggcaaa
    12061 agtcttctgc tcgctggctg actggatcaa ccacagcggc caattctaag acatctgatc
    12121 ttttttgata ataagcagca tgtacttttt ctgctacttg tccagcgcct atgattgcta
    12181 ctttcttcat ttatttctct ctcctttgct tatttactta ttgttattct ttctctattc
    12241 tttctctaaa tcatttaaat gacgcgctgt ttccagacag tttttcaagt attgcaatga
    12301 ttcctgataa attttcactt cattttctcc tcgcaaacga cattcatata ccatataacc
    12361 ttcatactca ttttgaagta gcgtttgaaa atggcgagta aaatcaattg cgccagatcc
    12421 tggttggtag cgatgattat ctgctaaatg gatatgaccc acatactctt tttgcgtagt
    12481 caatgctttt gaaatatcat cttcttcaat attcatatga taaaaatcag cgatgatctg
    12541 cacatgtttc aattgattag agtcaatgta atgacgcgca tccgctaaca aattgatcat
    12601 atggtcttgg tatcggttca aaggctccaa ataaataacc gtacctgttt cttcggctac
    12661 tttatctaaa taacgaagtg attcgctgac tgctttatag tctccttctt tgcttcgggg
    12721 tgaaaccatc ggcggtaatc gataagtgaa cattccccaa gctgctggga taacgattcc
    12781 tttgcctcca acctcgctta gtgcacgcaa aatctcagca atttgacgta caccattaag
    12841 acgccgttct tcgataaagt caccgatcca cccatcgtag ccgttacaag ctgtcactac
    12901 tgggatccct gtttctttga ttgcctgttt gactgtgtcg atattttcta ttaacaactt
    12961 accgtcgatt tcgtattctt caaatccaca gtctttgata aatctaaatt tttctaaaat
    13021 gtctcttggg aaaaatgctt gatcttgcgt accgaatttc attgttctcc tcctcggact
    13081 tgaacgccca ttttgatact ttcttctggg tgctggtcta cataacgcat gtaagcttct
    13141 gcgctttctg caaatggcac aactggatca atgatttctt cacaatccaa gtaaccgttc
    13201 atcaataatt cccaacatgt gtcctcgatt cgtttacggt tccatcttgg gtaatctggg
    13261 tttggttcac tagatgctcg agcaaagacg atttttgcat tattgaaatg tgcttcgcga
    13321 ccaagattaa acccagctgg aaaaggttta gcaaaggcaa cataagaaat gatcccgcca
    13381 tagccgatcc ctattaaaga agcttgtaat gcggcagcac tgccactcgt ttcaataact
    13441 gaatctactc cacgtttatc tgtcaattcc tttagacgaa aaccgacatc tgctttcata
    13501 ggatctagcg tgtaatcggc accgtgtttt tctgctaatt ctctacgctg cgctatggga
    13561 tcgatcccaa cgatggtagt agcacctgct tttttagcta attgaatcgc aatttgcccg
    13621 atagcgccta aaccgaagat tgctacagca tcacctgctc gaacatgtgc ttcccggata
    13681 ccacctaaag cgaattgagc aggatcgtaa catactgcat ttttccattg atccaaccga
    13741 tcaagtttac gtagtttata attatcaaca ccgtttgtaa tcagcgtttc acgaatgggt
    13801 ccataagtgc agactaactc tcctttttga tactctgtca ccaaactgcc cgtttcttcg
    13861 atcgtaccga caatcatatt tcctaattga aattcgccaa ataccacacc gcgttggctt
    13921 ccttcttctc ttggcaagaa taggcgccat tcttctgaaa attcttcatc gataaatgga
    13981 cttaatcctc taaagtcgac aatttctgtt cctggttttg gagtagcgaa aattactcgg
    14041 atacgtactt cgttcttttc gatctcacgg tcttcgtaat ctttgaatcc ggctattctt
    14101 ggttctaatg caactaattt tttcatcttt tctttcctct ttcttttctt tttatccttt
    14161 tacgccgcct tcgactaaat cacctttaat aaatcgttca gataacgcat acataatgat
    14221 gactggtaaa gcggtcacca atgaagcggc catcattcgt ccccaaatgt agtctggtgt
    14281 actgaataat gtgttcaaac cgattggtaa agtaaatttt tcggaggtcg acaggaaaat
    14341 cgaagcaaat aaataatcat tccacgaaac cataaagcaa taaacgaaga cagaaatgat
    14401 tccagaaata gccaatggta taatgatccg aataatgatc tggatccggc taagaccatc
    14461 gatcattgct gcttcttcaa tatcagtcgg aatcgtgtca aaataacttt ttagcatgaa
    14521 tatcgcagta ggaagtgttt gaacgaccat cgtgataatc agtgctgtat tggtatcata
    14581 caaacctaga ccggaaatga ttttaaataa tggaacaacg atcaaaatac cggaaaacat
    14641 ataaacggtg taaaagctag cattgatttt cgagcgagct ttgaagcgta gttttgacaa
    14701 agcgtatgcc ccgctgatcc caatcactac tgcagttacg gaggctatca aggagatcac
    14761 taaactattt ttgaaataca tgatgaatgg aaaaatctca gcattgaaaa tatcctgata
    14821 atgttcaaaa gtccattctt tcggaaaaat agtttgtgtc gttgcaattg cttcttgtga
    14881 atccttgaat gatgtcatca gcatgatcaa aaatggaaac aaattgacag ctaatagcaa
    14941 caaaatcgtc gcgtaaaata agattttcct gctcttttta tttttcattt caagtccacc
    15001 ttctttcgtg agagcaagac gaccgctgca ataatgatga acaatagaat cgatatagct
    15061 gctgcttttc caagatcatt gaacgcaaag gctgtctgat acaaataaac gcctaagata
    15121 ttgactttat tcgtcagtag atagacatct gtaaacatat agaacatcca gatagatcgt
    15181 aaaacaatca cagttgctag tactggtttg attgccggca gtgtcacgat cttgaacttc
    15241 tgccaagcat ttgcaccgtc catttcagct gcttcataaa gcgttttgtc gactgtttgc
    15301 aaaatagcca aaaatgaaat aaacgcatac ggatagtatc tccagatagc aaacaaaacg
    15361 accataaaga agctagcaac tggctggtcg aaccacagcg gtgcttcgtt gaaaagctgc
    15421 aacctatcta ctaacagatc attgacgatt ccataactat tattgaacat atatttccaa
    15481 gcaaaaatca aagaaataga tggcgtaaca taacttaata aaatcagtga acgcaccgtt
    15541 ttcctaaatc tgaacttgcg gttaagcaaa atagctacgg ctaatcctaa agctgtactg
    15601 cctgctacaa ctaagaatgt gtaagcaact gttaaaccta aagaacgata aaattcacta
    15661 tcacttaaaa tttcaagata attgcttaat ccgataaaaa cggcgtctga gttaggattc
    15721 agcggcatat cgaaaaaact gatctgaata ttggaaagca ttggaatcag cactaaaaca
    15781 gctaataaaa gcatgcttgg tgctaaaagt cccatggcta attttaagtc agaatgtttc
    15841 cttggacgat tcattgaaac ctccccctga taatctgcca atagctatct acttttttcg
    15901 aacaccattg gcagaacttc ttgttatttc gtattttttt gtgcgtcagc taaagcttga
    15961 tcgacatctt cgtttccgac agttacttgg ttcactgctt ttccgatcac acctgagctt
    16021 gtgatatctc ccattttcgt gaaattcttt tccccgacta gtccaaagac ttgtacttca
    16081 tcaaaagcac tagcaatctc agatggcagc tcgccaaaag acttgatgac ttcattttct
    16141 ttgtaaccat ccagttcgac tacttgttta ttcactggtt gagcaccacc aggagacatc
    16201 aagacccact tttccatgtt tttcggttga gataagaaag ctgtgaatgc ttcagcagcc
    16261 tctttttgtt tctcgtctag accagaagaa atcgtcaatc cagatactgt gccatagaca
    16321 gcttgatctt tttgtgttgg gatagcaaaa ccgatatttt ctgagttccc cgcttcataa
    16381 acagaaggca aaatgtaagt tgaatagatt gccattggtg ctgttccatt catgaaagca
    16441 tctttgattt ctgtcacatc attagagcct ggcatcgtgt attgggataa gttttgataa
    16501 aacgacaatg cttctctcat cttttccgta tcgatcgtta cctcgccttc atcatccaaa
    16561 acatttgctc catttgacaa agcaaattga gagaacgctt gctcactcat tgttccttca
    16621 gcagtaggca tagcaattcc atattgttta ttctctttat ctgtgaaatg ttgagcaatt
    16681 tttaaaatat catcccaatt ttctggttct gaaaaaccag cttctgataa tttttctttg
    16741 tcataccaaa taccttgaac ccagccgcta attggtgcag caatatagct ttttccatct
    16801 tctgaacgaa caaggttttt ggctccgtca taataattat cttctccagc ttcctcgata
    16861 acgttttgga ctgccgtctg atcaatcagt tcatctttat ccatcacttt agcaaaatcc
    16921 tgactgactt ccattacttc tggtaattta ccggaacgag ctaacgtaac gatttttgta
    16981 ttgaacgctg attcttctac cggcacttgc ttgattttga tgtctggatt ttctttttca
    17041 aaatcagcaa ccagttcatt gatcacactt aatcgttcct gttcgacaga agagtgcatg
    17101 aattgaatcg ttgtctgttg tgattcgtta gagctttgac tttcaccaca agcagtaaga
    17161 agaaatccag tagctagcag acaggtagta acaataattt ttttcatttt tttccacctc
    17221 ttattttatt attttcagcc acatatattc atacggctgg aatatcaact ttcgttcttg
    17281 actgcaatgg ataatttgtc cagaaaacag gtctaggtaa tctccttttg gtaaaagata
    17341 acctgctgat tgattggaaa aattatggat aactaagact ttctcttcct tcgcttcgcg
    17401 aataaaactg ataaaagaat ctgctgagtc cagtaagtaa atcaaactat tcggatggaa
    17461 ggctttttct ttttttcgaa tagatagcat ctgggtcaat tcaccatata ctgctgttct
    17521 taatggatct gtctgcaact ctttctcaat atctgctaac aaaaattttt gccgattgat
    17581 ttttcgattc tctcctgttt tttctacgcc tttgtaatca tttcgacttc ccagcacact
    17641 ttgaatataa attgccggca cgcctggtaa cgttaccaaa aatgaatgag cagctaaaaa
    17701 gcgtttgctt ctccattccg tcgtatctgt tggagaactt aacgcatcta gatacgtagc
    17761 gtttatctca tatggactgg tcgttccatc tgggtttttc ttgtagttta ctcgggcacc
    17821 ctctttcttc agctgttcca ctaattcaag gatcgttttt tccggcagaa tacctcgtac
    17881 cggattcaat ccgataccgt catgggaggc taaaaaatta aaaaaagttg tttttttcga
    17941 tgcgaaatcg atggttttta accattcttt caacttactt gtattacccg aacgaatcgc
    18001 ttgaagggtc aaaggtggta gcgaaaactg atagaccatc tgtgcttcat tctcgccatt
    18061 cccaaaataa ctgatattat ctttgtgtgg cacatttgtc tcagtgatca ataccgttcc
    18121 tggagctgct tcatcagcga ttgcacggaa taattggatg attttatgcg tttctggcag
    18181 gtgtatacaa gaagttccta gttctttcca taaaaatccc acagcgtcca agcgaataaa
    18241 tgaagcgcct ttttctaagt aaaacaaaag aatatcgatc attttcaata aaacttctgg
    18301 atttttaaaa ttcaagtcta cttgatcttc gctaaaagtc gtccatacgt gtttttcttt
    18361 cccgtttttg tcaatgaacg ttgtaagtaa cgggctggtc cttggtctag tcacgctgct
    18421 tgtatctgta tccagtggca aatcaataaa aaaatctttg tacgtatcat tcaagtttaa
    18481 atattcctga aaccaagtac tttcggcaga catatgattg cagacaaagt cgaacattaa
    18541 ccgtgtttcg cttctcattt cttctatatc ttcccatgtg cccaaacggt cgttgattgc
    18601 ctgataattt acaacggaga aaccatcatc agatgtatat gggaaaaatg gcaagaaatg
    18661 tacaactcta aatgtatctg ccaaatactt ttgataaaat ttcttaaatg tacgcaaagt
    18721 tggttgctgc tcttcaaaaa attgatcccc ataagtaatc aagaaaatat cttcttcgtc
    18781 ccaatgcagt ttgcctgcct ggcctttctt ctgctgataa acactgactt tttgttcaat
    18841 catagaggcg gttttctcag acaaatctga gtcgcgataa atcgcttgta aaagttttgt
    18901 atacatcgga ttcccctttc ttttttggga gcgttcccaa cgatttgatc atattatgga
    18961 aaacgcttac tgtcaatagc atttttttaa ataattttga tatacacaaa aatacaaata
    19021 agaaagctta tgtatcaata attaatagct tcttaaaaaa atatggtctc tcatgatcat
    19081 gcaagctatt cctaaatttt caaaatcata gacaaaaaag aatgtctatt gtactatgga
    19141 attattctaa aacgctccca aaaaggaggt gcatataaaa ttgaaaccta ctatttatga
    19201 cattgcgaaa aaagctaatg tctcaaaatc aactgtctcc agagtattaa ataaccagcc
    19261 aaatatctct gataaagcaa aagcaagtgt acttcaagca atcaaagaat tgaattatga
    19321 accgagcaaa attgcaagag gactgtcagg aaatggtttt gacgcgattc tagcattatc
    19381 tcatcgtact tctcatacga cctatggcaa tccttttttt tcagatgtta ttcaatccat
    19441 ttctgccgtt tctgaacaaa ataacttcga cttgattcta caaacggcaa aaacaccaga
    19501 agaagagcta gaaaaatgcg cgaaaaagat ccaagaaaaa atgatccgag gaattattat
    19561 tttaagctct acgatggatg aaacacttct ttttgaattg gatcaatata atattcctat
    19621 tgtggtaatc ggaaaagtcg gaaacgacta cacgaacatc tactcagtgg acacggataa
    19681 tttccaggat agttacgatt tggtggaaac actgatcaag aatggacacc gtcatatcgg
    19741 ctgtctccat gctccgacag aagtttatgt atccaatgat cgtctcaatg gttaccgtca
    19801 atgcttattt gatcaccacc tagatatccg taatgaatgg attatagacg gcggctttcg
    19861 cttagaagat agcatggcag caatgaatga tttaatgaag ctacgagaaa gaccaactgc
    19921 gattttttct accgatgcat taaaatcatt gagtatttac aaagtggcag aacaatctgg
    19981 aaagcagata cctgatgatc tttcgatcgc aggattcagt gatcccttat tgtcctccct
    20041 attttcgcca cagttgactc ggatagacat tccgacaaat cagttaggaa tcaaagccac
    20101 agaactttta ttccaactaa tccaccacca ataccctgaa gaaaaaagaa tcattctttc
    20161 aaccgagatt actctcacag attcgatcca acaacaagaa atgtagaaca gaattataaa
    20221 agaacagaaa cactgaggat aagtcagtta ctctgttctt ttttcaatca ctcatccctc
    20281 gttaccccct cccggtattt tagcgaattt ttcttatttt ccatctgttt tagatagctg
    20341 tggataacta taatgtttgt gttaaactat tttttccaca gggaagaaaa aactattttt
    20401 aaccacgttg tgtataagtt tttatagttc gtttttttaa acacatttac ccttatttca
    20461 atccatgtta tttggggata cttatctcta aaacataaat agaataacag acatgtctac
    20521 tggttttgtt ttattcgagg aattattcaa taatagctag caaaatatta aaaataaccg
    20581 ctctatcttc ccatcaattt attttgcatt caaaaaatga tctatcttta cttttgttga
    20641 ttgacttctc ttggaagatg atcgttccga taccttaatt ggatacgaac cgatgttgtt
    20701 aagatccttt cccacttttc ttctagttag acaaattatt ttagacaaac caaaaaagag
    20761 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
    20821 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
    20881 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
    20941 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
    21001 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
    21061 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
    21121 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
    21181 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
    21241 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
    21301 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
    21361 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
    21421 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
    21481 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
    21541 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
    21601 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
    21661 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
    21721 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
    21781 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
    21841 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
    21901 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
    21961 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
    22021 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
    22081 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
    22141 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
    22201 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
    22261 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
    22321 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
    22381 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
    22441 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
    22501 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt
    22561 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
    22621 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
    22681 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
    22741 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
    22801 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
    22861 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
    22921 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttgag
    22981 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
    23041 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
    23101 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
    23161 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
    23221 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
    23281 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
    23341 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
    23401 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
    23461 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
    23521 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
    23581 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
    23641 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
    23701 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
    23761 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
    23821 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
    23881 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
    23941 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
    24001 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
    24061 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
    24121 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
    24181 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
    24241 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
    24301 aaatccgctc acagaacaac ccttcacgtt caggttttaa ggtacgatag ttaatcgtct
    24361 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
    24421 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
    24481 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
    24541 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
    24601 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
    24661 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
    24721 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
    24781 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
    24841 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
    24901 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
    24961 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
    25021 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
    25081 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
    25141 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
    25201 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
    25261 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
    25321 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
    25381 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
    25441 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
    25501 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
    25561 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
    25621 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
    25681 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
    25741 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
    25801 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
    25861 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
    25921 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
    25981 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
    26041 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
    26101 cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
    26161 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
    26221 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
    26281 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
    26341 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
    26401 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
    26461 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
    26521 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
    26581 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
    26641 cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca
    26701 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
    26761 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
    26821 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
    26881 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
    26941 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
    27001 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
    27061 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
    27121 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
    27181 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
    27241 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
    27301 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
    27361 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
    27421 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
    27481 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
    27541 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
    27601 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
    27661 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
    27721 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
    27781 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
    27841 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
    27901 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
    27961 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
    28021 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
    28081 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
    28141 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
    28201 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
    28261 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
    28321 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
    28381 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
    28441 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
    28501 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
    28561 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
    28621 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
    28681 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
    28741 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
    28801 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
    28861 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
    28921 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
    28981 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
    29041 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
    29101 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
    29161 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
    29221 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
    29281 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
    29341 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
    29401 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
    29461 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
    29521 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
    29581 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
    29641 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggcaaacaaa
    29701 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
    29761 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
    29821 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
    29881 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
    29941 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
    30001 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
    30061 aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
    30121 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
    30181 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
    30241 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
    30301 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
    30361 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
    30421 cggttatatg atttctaatg gatgcattgc tctttctggc tccttacttg cgcaaaacaa
    30481 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
    30541 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
    30601 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
    30661 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
    30721 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    30781 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    30841 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    30901 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    30961 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    31021 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    31081 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    31141 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    31201 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    31261 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    31321 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    31381 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    31441 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    31501 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    31561 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    31621 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    31681 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    31741 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    31801 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    31861 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    31921 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    31981 ttgacttctg aatctacgat gaaagatgcc aaactggcag cagctcaagg agattctgtt
    32041 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    32101 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    32161 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    32221 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    32281 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    32341 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    32401 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    32461 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    32521 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    32581 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    32641 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    32701 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    32761 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    32821 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    32881 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    32941 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    33001 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    33061 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    33121 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    33181 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    33241 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    33301 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    33361 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    33421 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    33481 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    33541 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    33601 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    33661 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    33721 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    33781 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    33841 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    33901 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    33961 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    34021 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    34081 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    34141 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    34201 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    34261 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    34321 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    34381 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    34441 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    34501 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    34561 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    34621 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    34681 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    34741 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    34801 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    34861 ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat actacctcta
    34921 ccacaagtga tagcacggat acatcagctt caagcgacag tacagatact acctctacca
    34981 caagtgatag cacggacaca tcagcttcaa gtgacagtac agatactacc tctaccacaa
    35041 gtgacagcac ggacacgtca gcttcaagcg acagtacaga tactacctct accacaagtg
    35101 atagcacgga cacatcagct tcaagtgaca gtacagatac tacctctacc acaagtgata
    35161 gcacggacac gtcagcttca agcgacagta cggatacatc taccacaaca agtgatagca
    35221 cggacacgtc agcttcaagt gacagtacag atactacctc taccacaagt gatagctcag
    35281 attcatctac cactacaagt gatagcacgg acacgtcagc ttcaagtgac agtacagata
    35341 catcctctac cacaagtgat agctcagatt catcaacttc aagcgatagt acagacacgt
    35401 cgtctaccac aagcgataca cgtcatgcaa gtgtctcctt ctcaaaaaca agttcggata
    35461 acaggacgaa ctttaactta actaataaaa caagcaatga caatggaaac aaatcaaaaa
    35521 gagcacttcc taaaactgga agtcaatcga ataattggat aacactcgca ggcgttatat
    35581 tacttgtaat cggtttaaga atcactttta tgagttataa gaaatcgaaa aggtaaaaaa
    35641 atattcaaga taaaaaagag caacgtcgct aacggacgtt gctctttatt tttgcttttt
    35701 atcataaata caagaactac aattcaattt gtcaaaaaac acgtagattt ccttctgaga
    35761 aatttattta cgtatcgaac ctttccctat tcagtctttg cgatccaaaa tcagttgggt
    35821 aagggaaaag agattctctt ttgttcgaag agaggtggca ggtaatttct ttatttcctt
    35881 caaagccttt tctgtgtaag aggccgctaa cttcctagct tcttccacgc ctcctgtatg
    35941 ttcaacgatt ttgcgtacgg cgtcgacatc ttcttctgtc attgccgctt tcttctctaa
    36001 ataaggtagt agctttggtt tgtttttttg caaagagtag atcaacggta aagaatagac
    36061 cccttgacgc atgtcttcta gtacaggttt gccgatttcc tggctctctt ttgtataatc
    36121 taaaatatca tcgataatct gaaaggccat cccgatagaa agtccgatat ctcctgcttt
    36181 ttttgcgaag cgctcgcttg ttccactttc aaaagcgcca acagaacaac ttaattggaa
    36241 cagttcagct gtcttacctg aaatattttc caaatattct tttacagaaa gttcataatt
    36301 atagcgatca tccatctgcc ctaattcgcc attcaatact ttttccatac tgcgagaatt
    36361 caattggaga cttttcaagg aagtggagta atcagacaat agtttaaaac agcagacaaa
    36421 taaatagtct cctgcataga cagaaacgac attaccaaac tgactacgga tattaggctg
    36481 accacgtcgc agatccgctt catcaacgat atcatcatgg atcagtgtgg ccgtatggag
    36541 catttcgata gaagctgcca aagcgatggc tttgtctcga tcttgctctg gtccaaattg
    36601 tgagaacaat agctgatagg caggacgcaa cagctttccg ccggcatgga tcgtttcttt
    36661 gatggcttgt tcgacaggtt tgtttttcag gcggacagtt tcttccatcc gttttagtgt
    36721 ttctgtcaat tctttactta aagcaggata gttttcccac attggatgta gcttcataaa
    36781 aaaactcctt gtgtgacgat tattttttgg taaacattgg gctccatttt tgtttggttc
    36841 cctgctcata atctttttgg aatcgttgaa gtgtctgcac atgtcgtaaa agataattcg
    36901 ctgcgatccc gattgcaatc cctgataaaa tccctaaaac tgaaaggact ggcaagtaca
    36961 acatgactgt ccatgattgt gcaatgaaag aagccatcaa aagctgtccg acattatgca
    37021 tgaaaccgcc ggccgcacta attccgatga tactgacacg ttttggtcct aattgcttga
    37081 ccaataacat tccaaaataa ctaagcaaag caccactcat actataaagg aatgtggaaa
    37141 tcgttccgcc tagcaaagta gtcaggaata gtcgtagcca aagtagtaag aagctgtctt
    37201 gttttttcat ggtaaaaatg gcaacgatcg tgattagatt ggctaagcct agttttgcac
    37261 caggcgcaaa agaaaaagga taagggatca tattttccaa taaaccaatg atcactcctt
    37321 gtgcaacaag tagcgaaata taaatcattt tttgtaattt actcatggtg tgtgaaggtc
    37381 taataaatca gactgtcttc gtcacctcca tctgttgaac ggacttcgat cactaattta
    37441 tgtggcaaac agacgatggt ttcgccattt ttcgttgccc agcctcgacg tacgcagatc
    37501 tggtcatcgc aatctgcttc ttttatccgt actcgatcgc catctatctc gatcaagtta
    37561 taatctccgt gttcatcttt gtaagtatat gtgttgctct cttctccagc catcaaaggg
    37621 aatgttttga tttctgtgcc atccacacgc agtaccgctt ctttttctgc tgttgcggtg
    37681 ttttgctgaa gaccaaaaat catgactggt aaaaaagaaa gaacagtcaa aagcaaaata
    37741 ataaaaacat cccaaggttt gagcagactt ttttcagcaa attctttaag gctcattttt
    37801 gttctccttt ttccagatta actcacccaa ttttagcata aaaacaagag gttgtgacac
    37861 ctgtgtcttg ctttgagtaa taagaacgaa gaatagaaaa cagctttcca tgttttctaa
    37921 ttctttgggc ttattaccga gaagcagcag gtggaacacc gtttataaca agaggttgtg
    37981 acagaagtgg gcaacttcaa gaaataagaa ggaatttacg aaaattactt ttcaagtttt
    38041 tgtgaaattt cggcttattt ttcgaagaag ctacttctgg aacaccgttt ataacaagag
    38101 gttgtaacag aggttgtgac acctgtgtct tgctcatgga ggcttatctc gggcatctta
    38161 ttcctttaat tttacataaa aaggaggctg tgacggtttg tctcagcccc tttctattga
    38221 aatcttgcat tcaaataacg ttctttatct atttatttct tgccgtactg ggaacttggt
    38281 ctaccgtacc accaattacc tagtgtacgt tgtagccatg gaattaccca gcgatccaac
    38341 ccaatcgcac gtccggaacc gttcatcaat gcgaaagcaa caa
//
LOCUS       DAZUKZ010000017        38337 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000017 DAZUKZ010000000
VERSION     DAZUKZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.017"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            281..601
                     /locus_tag="ACG8EF_000817"
     CDS             281..601
                     /locus_tag="ACG8EF_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFS4946164.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            755..1660
                     /locus_tag="ACG8EF_000818"
     CDS             755..1660
                     /locus_tag="ACG8EF_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4946165.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1657..2430
                     /locus_tag="ACG8EF_000819"
     CDS             1657..2430
                     /locus_tag="ACG8EF_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4946166.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2611..4035)
                     /locus_tag="ACG8EF_000820"
     CDS             complement(2611..4035)
                     /locus_tag="ACG8EF_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFS4946167.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4246..4692
                     /gene="nrdI"
                     /locus_tag="ACG8EF_000821"
     CDS             4246..4692
                     /gene="nrdI"
                     /locus_tag="ACG8EF_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS4946168.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4757..6739)
                     /locus_tag="ACG8EF_000822"
     CDS             complement(4757..6739)
                     /locus_tag="ACG8EF_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS4946169.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6753..7649)
                     /gene="murQ"
                     /locus_tag="ACG8EF_000823"
     CDS             complement(6753..7649)
                     /gene="murQ"
                     /locus_tag="ACG8EF_000823"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS4946170.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7652..8713)
                     /locus_tag="ACG8EF_000824"
     CDS             complement(7652..8713)
                     /locus_tag="ACG8EF_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS4946171.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8924..9451)
                     /locus_tag="ACG8EF_000825"
     CDS             complement(8924..9451)
                     /locus_tag="ACG8EF_000825"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4946172.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9518..11020)
                     /gene="eat(A)"
                     /locus_tag="ACG8EF_000826"
     CDS             complement(9518..11020)
                     /gene="eat(A)"
                     /locus_tag="ACG8EF_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS4946173.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11278..12399)
                     /locus_tag="ACG8EF_000827"
     CDS             complement(11278..12399)
                     /locus_tag="ACG8EF_000827"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS4946174.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12525..13466
                     /locus_tag="ACG8EF_000828"
     CDS             12525..13466
                     /locus_tag="ACG8EF_000828"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4946175.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13616..14512)
                     /locus_tag="ACG8EF_000829"
     CDS             complement(13616..14512)
                     /locus_tag="ACG8EF_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS4946176.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14781..16121
                     /locus_tag="ACG8EF_000830"
     CDS             14781..16121
                     /locus_tag="ACG8EF_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4946177.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16339..16782
                     /locus_tag="ACG8EF_000831"
     CDS             16339..16782
                     /locus_tag="ACG8EF_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFS4946178.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17286..18704)
                     /gene="nhaC"
                     /locus_tag="ACG8EF_000832"
     CDS             complement(17286..18704)
                     /gene="nhaC"
                     /locus_tag="ACG8EF_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS4946179.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19107..20330)
                     /locus_tag="ACG8EF_000833"
     CDS             complement(19107..20330)
                     /locus_tag="ACG8EF_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4946180.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20361..21200)
                     /locus_tag="ACG8EF_000834"
     CDS             complement(20361..21200)
                     /locus_tag="ACG8EF_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4946181.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21217..21897)
                     /locus_tag="ACG8EF_000835"
     CDS             complement(21217..21897)
                     /locus_tag="ACG8EF_000835"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS4946182.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21899..22972)
                     /locus_tag="ACG8EF_000836"
     CDS             complement(21899..22972)
                     /locus_tag="ACG8EF_000836"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS4946183.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23028..23266)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23309..23542)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23619..24290)
                     /locus_tag="ACG8EF_000837"
     CDS             complement(23619..24290)
                     /locus_tag="ACG8EF_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4946184.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24377..27025)
                     /gene="mgtA"
                     /locus_tag="ACG8EF_000838"
     CDS             complement(24377..27025)
                     /gene="mgtA"
                     /locus_tag="ACG8EF_000838"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFS4946185.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27367..27541)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27714..28151
                     /gene="ndk"
                     /locus_tag="ACG8EF_000839"
     CDS             27714..28151
                     /gene="ndk"
                     /locus_tag="ACG8EF_000839"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFS4946186.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28173..29039)
                     /locus_tag="ACG8EF_000840"
     CDS             complement(28173..29039)
                     /locus_tag="ACG8EF_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4946187.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29199..31292)
                     /locus_tag="ACG8EF_000841"
     CDS             complement(29199..31292)
                     /locus_tag="ACG8EF_000841"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4946188.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32041..32664)
                     /locus_tag="ACG8EF_000842"
     CDS             complement(32041..32664)
                     /locus_tag="ACG8EF_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946189.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32823..34220)
                     /locus_tag="ACG8EF_000843"
     CDS             complement(32823..34220)
                     /locus_tag="ACG8EF_000843"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4946190.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34252..35223)
                     /locus_tag="ACG8EF_000844"
     CDS             complement(34252..35223)
                     /locus_tag="ACG8EF_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4946191.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35597..36022
                     /locus_tag="ACG8EF_000845"
     CDS             35597..36022
                     /locus_tag="ACG8EF_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946192.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(36510..37211)
                     /locus_tag="ACG8EF_000846"
     CDS             complement(36510..37211)
                     /locus_tag="ACG8EF_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4946193.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(37227..37667)
                     /locus_tag="ACG8EF_000847"
     CDS             complement(37227..37667)
                     /locus_tag="ACG8EF_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS4946194.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(37667..38242)
                     /locus_tag="ACG8EF_000848"
     CDS             complement(37667..38242)
                     /locus_tag="ACG8EF_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFS4946195.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT        11073 a         8003 c         6682 g        12579 t
ORIGIN      
        1 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
       61 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
      121 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg
      181 ccaccgggta ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct
      241 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
      301 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
      361 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
      421 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
      481 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
      541 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
      601 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
      661 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
      721 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
      781 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
      841 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
      901 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
      961 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
     1021 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
     1081 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
     1141 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
     1201 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
     1261 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
     1321 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
     1381 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
     1441 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
     1501 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
     1561 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
     1621 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
     1681 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
     1741 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
     1801 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
     1861 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
     1921 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
     1981 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
     2041 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
     2101 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
     2161 aatagttccg gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
     2221 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
     2281 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
     2341 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
     2401 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
     2461 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
     2521 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
     2581 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
     2641 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
     2701 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
     2761 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
     2821 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
     2881 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
     2941 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
     3001 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
     3061 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
     3121 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
     3181 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
     3241 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
     3301 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
     3361 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
     3421 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
     3481 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
     3541 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
     3601 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
     3661 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
     3721 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
     3781 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
     3841 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
     3901 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
     3961 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
     4021 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
     4081 aaaccttttt tatttttact taattaataa aaactttaat agaaatataa ttctaccggc
     4141 ttttttctca ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac
     4201 ttaaaaaaga tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat
     4261 atatcgattt caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa
     4321 atgcatgcag aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt
     4381 gattttacga aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga
     4441 aatggtttag ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa
     4501 catgaagaca atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat
     4561 aatcagtatt gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca
     4621 gattatgaat tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa
     4681 aacaataaat aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc
     4741 tattgtttct atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac
     4801 ctgttttttc caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg
     4861 atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag
     4921 gtgtcacttg ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg
     4981 tgttgattcc catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt
     5041 gtttcgttgg gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt
     5101 ctggttctac aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt
     5161 tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg
     5221 aagtttgagg tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt
     5281 cttctttttc taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag
     5341 cataagctgc caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga
     5401 tcaaagcggc tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca
     5461 ctgccccgcc gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg
     5521 ttactccgta aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga
     5581 tcatttctgt taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca
     5641 cttgtcctgc accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa
     5701 tcatttgcac atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga
     5761 ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc
     5821 caccgaccat gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca
     5881 gaccgatggc aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta
     5941 ctttatgcag ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga
     6001 tcccaccttg tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg
     6061 gattcatccc tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac
     6121 caaaaacttg tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt
     6181 tcaagatgtt catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg
     6241 tgactaagtt tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag
     6301 gaatcattgg cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt
     6361 tttgtttcga tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc
     6421 cagatgctga tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca
     6481 cgcctgccaa atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga
     6541 cttgaagctg ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga
     6601 catttacaag atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc
     6661 tttctacatt gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag
     6721 tcagttcgtt ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt
     6781 gcttcagcta attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata
     6841 gccagcttaa cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct
     6901 tctgtcgctt gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg
     6961 acatcgacca tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc
     7021 atattcaaga taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat
     7081 tctggacctg catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta
     7141 ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc
     7201 acatatggtg tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc
     7261 agcttgatta gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca
     7321 gtcattgctt cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat
     7381 gtcggtacgc attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata
     7441 taaatcagac gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata
     7501 cttgtcagtt ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga
     7561 acggcttgag aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt
     7621 gttaggttct ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat
     7681 agttcatacc agctttgatt tggttgatca atggtagctc ttctttgata actttagcta
     7741 ctacattcac tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat
     7801 agcgcaaata cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc
     7861 ttgctgggat aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg
     7921 catcgtcttg ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag
     7981 gttccgtatg gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt
     8041 tttctgacag acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta
     8101 gagcgcctgc aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata
     8161 gtgggcctct caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt
     8221 tgagccattg attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat
     8281 aataattatg ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga
     8341 tatctttagg cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg
     8401 caatctgctg attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca
     8461 aaggcgttag atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga
     8521 ccatcaattc tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat
     8581 atgccgaaac atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg
     8641 atgccatttg gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg
     8701 aaaagccaaa catattatcc ctctttttct gtatccgtct tcatttctat gactagagta
     8761 tatatctaaa gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa
     8821 ggttgagttt atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa
     8881 gcagtgcctt gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca
     8941 attctgtatt atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag
     9001 cattgatcac tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt
     9061 cgatgcattc tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat
     9121 ttgggaatcg tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca
     9181 gtccatcata atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc
     9241 tgagacgatc agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga
     9301 ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct
     9361 gtcctttttc agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag
     9421 ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc
     9481 ccttaaccat accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa
     9541 tctcagtaga tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt
     9601 tcacgttcaa gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg
     9661 gttcatccca tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt
     9721 tccgttggcc catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa
     9781 gctttcggag gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg
     9841 ccaacgttcc tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg
     9901 gctggtattt ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg
     9961 atgactttcc tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa
    10021 aatgaattgg ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa
    10081 gtcgcttatg gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt
    10141 ttaatagttt ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc
    10201 gtttcatcgt ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag
    10261 tatcgatgaa tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg
    10321 ctttttcagc tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag
    10381 ccatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat
    10441 aaagttccag ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc
    10501 cccggtcatg gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt
    10561 gttttctacc agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt
    10621 catccacaaa taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga
    10681 attctctcca taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc
    10741 atatctcaaa atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg
    10801 tctgttgcgg gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt
    10861 tgttttgtag aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta
    10921 gtttccattg tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt
    10981 cgtagccgaa tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa
    11041 aagaccagtc ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa
    11101 agacccatac atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct
    11161 ccacaaggta cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat
    11221 ccctcacttt gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta
    11281 cttcgggagg atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa
    11341 tgtttgcctg tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac
    11401 aattgggaga aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat
    11461 tattggtttt cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat
    11521 cgtttgctga tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt
    11581 ttccaatcca gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa
    11641 aaaaagggct ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac
    11701 ctcccagaaa tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag
    11761 cgtcgtatct attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt
    11821 atatggattg gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct
    11881 tcgatcaaaa tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa
    11941 agaagcaccc aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa
    12001 agtgacaata atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc
    12061 ataactggaa gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa
    12121 tgcttgtgca gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct
    12181 gatttttcgc tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag
    12241 cgcgtaatag attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt
    12301 tcttccttta cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc
    12361 acttccttta aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct
    12421 taagtaagac actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt
    12481 ctatatacta aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag
    12541 ctggggccgg agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg
    12601 tcctcttgat cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg
    12661 ctgactttaa cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca
    12721 tcgaaagcaa cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg
    12781 atatgttttg ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa
    12841 atggattagg tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg
    12901 ggatcacgat gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg
    12961 gagaaatcga actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag
    13021 aagtctttca agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt
    13081 ggcgaaaagc ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata
    13141 ttgctgaact aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat
    13201 ttgcttctat cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata
    13261 ttgcttctac ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc
    13321 tgatcaaaaa ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag
    13381 gacaaaaata tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa
    13441 aagaaggaat ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag
    13501 ctggttctct ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc
    13561 cataagaatt gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt
    13621 tgttttcaac agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga
    13681 taaatcgttt tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct
    13741 taaatggtgt atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa
    13801 tattttcagg acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct
    13861 tttgaaaata catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca
    13921 gttccagaat cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc
    13981 caggttgttc attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta
    14041 taaattcctg cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa
    14101 atagatccgt ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat
    14161 tatgtttcag cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg
    14221 ccggttcatc ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa
    14281 aaaataacac aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa
    14341 atccataatc taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa
    14401 tgaattccga ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca
    14461 aaaaataaag tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg
    14521 cctcactttc tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa
    14581 aacgtttcct atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc
    14641 attcttctat taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg
    14701 ttatcattag tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat
    14761 ttaaaagaaa gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac
    14821 atatcaccat gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg
    14881 aagcgattgg gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt
    14941 tatatatcgt gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg
    15001 gattatctgg tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg
    15061 tctactggtc tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt
    15121 tgcagttgtg gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa
    15181 ctacgatcat caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt
    15241 ttgctaaaat cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc
    15301 aaatgttggg cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta
    15361 ctcctcatgg tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg
    15421 gttctgaact gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta
    15481 aagcaatcaa aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc
    15541 ttttgattct ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg
    15601 tatttgaaaa aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg
    15661 ctttgttctc atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt
    15721 tgaaggatcg aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc
    15781 aacgtgctgt tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg
    15841 tcggtgacaa attatttggc tatctggcag gttccctttc gtatacagta ttattgatct
    15901 ggatcattat cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata
    15961 aaggaatcag cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca
    16021 cgaactccat tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt
    16081 atcaaaaaaa gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat
    16141 tttatttatg tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg
    16201 caactccttt ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc
    16261 tctgtgcaaa ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa
    16321 aaaaggagtt tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca
    16381 acggtagtta tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc
    16441 agtcgtacac gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg
    16501 ttgttcatgt ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt
    16561 cttcttgttt ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca
    16621 gcttggatga aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct
    16681 gatttaaaag atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa
    16741 gagatcgaca aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata
    16801 aaaaaagagg atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga
    16861 acgccgtgaa taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa
    16921 caaaaatagc ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt
    16981 gaacaccgtt tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa
    17041 atggaaaaat agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat
    17101 tgaacaccgt ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat
    17161 tttgtgaaat ggctttccac atttttgtat aaatttggct taataccgag aaactggctt
    17221 ttgaataccg tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta
    17281 tgtttttatt gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt
    17341 tttgagattc cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg
    17401 aaaaatacga atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt
    17461 ccggcaattc cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta
    17521 cctaatacag ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc
    17581 agaataacag ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat
    17641 gttgtaaaaa tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt
    17701 gagatcaagc caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac
    17761 acagtaggca tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt
    17821 ccagtatttg aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt
    17881 gggttttgaa taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt
    17941 gccggcacct ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt
    18001 gaaatagtaa agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg
    18061 gctttacttg tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca
    18121 gttgaccaca tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct
    18181 agatttgttg attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata
    18241 gctccaacaa ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc
    18301 ccaatcgttg acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca
    18361 acaaatacag aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga
    18421 gttggaatga tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata
    18481 aagattggga tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc
    18541 gggtatcttt ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata
    18601 ttaggtgaga cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat
    18661 acaagtgttg cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt
    18721 ttagttgttc ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc
    18781 gcgattcctc ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc
    18841 ggtaccacct taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat
    18901 ggactttcca tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct
    18961 gaacaaaaca cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat
    19021 aagatcattt gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata
    19081 actttcacac acaagtttat tttttactag aaagtagcgc cctcttgttt ttttcttgct
    19141 actatttctt gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca
    19201 agagctgtat tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt
    19261 ttagcagcag ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc
    19321 gtaggctgga ttgttggtat gacttggctg acatttccta catcagaaga accgagactt
    19381 gttctttgtt ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag
    19441 aataagtcat caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca
    19501 aactcaaaag tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca
    19561 tatactttat ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa
    19621 tctggaatga cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct
    19681 tttggcaatt gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa
    19741 gcatttactc ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg
    19801 tcgactggat cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg
    19861 cataacgcta catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat
    19921 ccgttctcgc ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca
    19981 tcaatgaatt gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc
    20041 ccgcatgcat gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga
    20101 ccgggtttat tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt
    20161 tctacccaaa acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca
    20221 tagttactta cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa
    20281 cgatattgga ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc
    20341 tcctatgcta gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat
    20401 ccttttgatg ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt
    20461 ttttgatacg ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga
    20521 cgtgcatggt catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca
    20581 tctacagcta caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt
    20641 tgggaagcat ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca
    20701 gtgggcgctt gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga
    20761 agagctctgc cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata
    20821 tcagctacat ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg
    20881 atcgatacta aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg
    20941 aaagaattca aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta
    21001 aatttcacgt attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat
    21061 acatctgtat cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta
    21121 tctgcttttc cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca
    21181 aaacgaataa tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag
    21241 ttgattactg ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat
    21301 cgttacatct gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc
    21361 tgcacctact gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga
    21421 gacccgaatg ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg
    21481 actggttccc atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt
    21541 ctcgatttga cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt
    21601 cgcaccaatc gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat
    21661 aatgaaaggg atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg
    21721 gttagctagt aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat
    21781 catcccgcca atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac
    21841 aaattcatcc ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt
    21901 atcttcctcc ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta
    21961 tttttacacc agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag
    22021 ctaaagtaac gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata
    22081 aaatattcgc tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct
    22141 gatttccaat ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag
    22201 aatgggcaat gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt
    22261 tggtcaattc ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc
    22321 ttccatcttc cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag
    22381 tgatcaggac gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct
    22441 gaagggtcgt tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt
    22501 cattcgccaa agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag
    22561 gataagcaga tgctttttct tccaaaccga caagttccag tagttcattc actttttgac
    22621 gacgttcctg tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag
    22681 ttcggctttc cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc
    22741 gcaattcttt gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg
    22801 ttggtttctg cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac
    22861 cgacgattcc ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta
    22921 tctctttttc tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc
    22981 ctccttatct aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt
    23041 ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg
    23101 atcatactct agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg
    23161 aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc
    23221 tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa
    23281 gtccttttac taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt
    23341 ttcggaagtt tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta
    23401 acggtcgaac agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc
    23461 agcttttctt acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta
    23521 ctttcctttt caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg
    23581 aaaagtcaag tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt
    23641 aacatttcaa taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt
    23701 tcttcgacgg tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct
    23761 gctcgtgaag cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct
    23821 tctaatcctg ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac
    23881 acttcgtctc cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata
    23941 tctgttgctc ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt
    24001 aagtctgcca atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt
    24061 tgataaaaat attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct
    24121 gctttcacta aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt
    24181 tcttttggga tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt
    24241 ttttctgtgt caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta
    24301 atcctttcca aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa
    24361 gaaccagcct tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag
    24421 ctgtcacaag cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag
    24481 gaagtggcat caagcctaat tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa
    24541 taattcctaa tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta
    24601 ctttcggtgt acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc
    24661 atcctgcatg gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac
    24721 ttccgcccac tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa
    24781 atatcgaact tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt
    24841 tttttggctc gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa
    24901 catcatagat cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga
    24961 acgtacttgc aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga
    25021 tatatttcat gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg
    25081 tcaaatcttt ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat
    25141 ctacagaaat accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta
    25201 aaaatccgac agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag
    25261 gattcaattt tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca
    25321 tttccgagat tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca
    25381 ctgaacgtgt aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt
    25441 tcagagcttt caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac
    25501 caatcaaaac catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg
    25561 tcttttgtgc gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa
    25621 gtacggcttc ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt
    25681 tagaaatgtt cagcatttct tcaatcgcgc cttttgtgat catttgtgtt ttaccagctg
    25741 gatcagcaac cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt
    25801 tttgataatt gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat
    25861 ctaacaaatt tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac
    25921 gatcatcttc atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca
    25981 atgtccccgt tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca
    26041 aatttttgat cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg
    26101 taacgatcat tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa
    26161 aagcttctag ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca
    26221 atgacataaa tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt
    26281 gaattggttt ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag
    26341 aaacaatgat tccttccgca cttccactga tgacattact tcccataaag acaagatttt
    26401 cgtaacttgt ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat
    26461 agctttcccc tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca
    26521 tatccgctgg tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt
    26581 ccataggaat ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg
    26641 atttcagttt ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc
    26701 tgacgataac catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg
    26761 ctaatacata ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg
    26821 gcgtgatgta tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg
    26881 cattttcacc gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg
    26941 atgcatggaa tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt
    27001 tttgtgtatc tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt
    27061 ctcgctgctt ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct
    27121 gagaacagat agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt
    27181 catccatttg ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt
    27241 tcgaatagtt atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg
    27301 cggcaacaaa aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa
    27361 aaagcttctc agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct
    27421 gcttttagct accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc
    27481 tctcgacgtt tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat
    27541 tcggttttcc ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt
    27601 ttttcatact ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc
    27661 tcattttcca cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa
    27721 gaacacttgt tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc
    27781 aacgttttga agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg
    27841 aattagcaaa agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg
    27901 attacatgac ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata
    27961 tcgtccgtaa gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag
    28021 gagactacgc gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg
    28081 cggtaaaaga gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag
    28141 cggcgaagtg atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc
    28201 agttttttct ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag
    28261 aattcttggt agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa
    28321 taatctgcca tcaatgtttc atagatcact gtacttcttt ctttatgtgc taagctccgt
    28381 ttcatatctt cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa
    28441 gtcaggttca ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt
    28501 cgcattcttt ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa
    28561 ggaaaatcag ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat
    28621 tcttcaatga ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt
    28681 ttttcgacca tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc
    28741 ctacggttat tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc
    28801 attcgtcttt ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat
    28861 tctgcaagtg tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt
    28921 ttcactgaat ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat
    28981 tgggactgtg tcaacttctt gttgactcga agtttcttga tcaattcacc atcatacata
    29041 atagttctcc tttttaattt aaatttttcc acattcaata aatagatacg ttaaaaagga
    29101 aaaatacacg aaaaaaataa aaaaatgttt ttttcataaa aaataccaac cttcaagcat
    29161 tagaatgttg tctaactact gaaggttggt ttgattgttt attgattatt tttttgctgt
    29221 tcaacgtctg ttcttgttac tggatataag ttggttgttg ttttattacc attttttgaa
    29281 aagacacttg tcgaatgttc gcctttttgc ttttcgattg cttcattacg ttccgcttca
    29341 tttttatagt catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc
    29401 aagagatttt cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga
    29461 tctttggaga tttcctcatc gattcctgct gctttcgtat cgatgatttc tcctgtctga
    29521 ttattgtaag cttttcctcc taggacggtg tatttgggtg tcacaaagtt cccatttcga
    29581 aaagcaacta cttgatcatg ctgagaagat aataagtctg taccaaaatg aagataatct
    29641 ttatcatcga ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccgtat
    29701 tcatgattga ctgtcccttt agtataacca ggcatatgga tcatcaatgg cactcgttgc
    29761 atctgtgcgt tgtcaaattc atcccatgta tctggatctt taccaagagc gcttgctaaa
    29821 tctttattat ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt
    29881 tggtaaatac cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa
    29941 tgtgccgttc gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg
    30001 aaattcttgt catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa
    30061 aacggctgtt gtagatgctc aagatattgg gcagattctc tgaataaatc tttatctaat
    30121 atcccataac ctaacgtttc gtcggattca tcaaaataag atcgatcaaa gaaattgtca
    30181 taacctaagt ttttatatac atgatctcta ttccaaaaac tagctacatt cccatggaaa
    30241 acagcgcttg tatagccttg cttttgtcca aggatcgctg gtgcggcttg aaagacattg
    30301 tctgacccaa gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac
    30361 atattttcag catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaagctg
    30421 atcgttgctt gattctggaa aatactattt aaaaacggtg tgacttcttg tccgtctacc
    30481 ttcatattaa tcaaaaactg ctggaaactt tctaaatgga gaacgatcac atttttccct
    30541 ttagcgatcc caaatgtttc gggatttggc tctgcataat gtttctttgt ataatctaaa
    30601 acttcatcaa tcccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg
    30661 attccgtcat aaatagtata cgcatcgatt cctagatatt tgacaatata tgaacggtca
    30721 aatgttcgct gcagcaattg tggacggttg gcttcgctca gcgctagatt tcctgaaaaa
    30781 ataagccctg aaagacaggt aacagcgata gccatcggtt tgccgacagg gttgcttttg
    30841 ataggtattt tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccagc
    30901 cagtagatga catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac
    30961 actttcgaaa agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa
    31021 ataatattca agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatagctaac
    31081 gctggctttg gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatt
    31141 gggttgatcc acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca
    31201 tagtaggcaa tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa
    31261 cgattttggc acaatttttt aagttgtgtc acgtatatca cttctctcta ttcacatttt
    31321 ttcttatttt gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct taagattttt
    31381 ttattctttc taaagattat tccgtccact tttcacagca taaaaccgct gcgatgtaag
    31441 cgattaaact aatgaagaga taaaagtctt gtggtgaccg cttcactcca cggtattcaa
    31501 aagccagttt ctcggtattc gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct
    31561 ccttatttct tgatgcagga cagctttttc acaacttctt ccttaaacgg tgttctcctg
    31621 ccaactcctc ggccacaagt cacaatattt ataaaacatg agaagctgtt ttttgaaatt
    31681 gttccttgtg gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat
    31741 cagctgaccc tcggtattag attatgtcag gacgctaatt ttacaacctc ttggtaaacg
    31801 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata taaggagcta
    31861 ttttttgaaa ttgctccttg tggctcagag ttaaacggca ggctcaagac ctcttgatac
    31921 acggtgttca atcagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggaact
    31981 attttgccgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctctgtttt
    32041 tcattgtcca taggattgat attgtccttg tgatgactca ttcgtaataa ttgaggcttg
    32101 attttcttca cttcccgtta tcgtttgtcc actgccagaa tctgaaggac ttgttcctgt
    32161 cgactcgttt ttttgagaag agacagttgt tttcttgact tgttcatttg tctttttcca
    32221 ttccgctaaa cgatcggcga ccactttttt gtctactttt tcagaataag cggtgatcac
    32281 ttttccattt ttcaaacggg ctactttgtc gccatcatga gtcaaaagca aaacggtcaa
    32341 atgcttgtct ttaaattgat attgtctgag agaaatcgca ctaagtacgt catcgatatt
    32401 aataaattga ttttcttctt ttccttttgg caaataaatg tccaattcgc ctttttcgta
    32461 attggcagtt attttatact ccgcccacca ataagttgag aattcttttt tgaaattatc
    32521 tactgattct ttgcttgcta cgattttttc tgtttctttc tgagcaagat ctgctgtcat
    32581 ttttcctgaa aatgctgcgg ataaaataag cagtaagaaa aaaccgaata accaaaagag
    32641 aagaaacggt ctttttttcc tcatgctttc ctccactcct atttaatttt gactattcta
    32701 ttatatacaa aaaatgaaat agaaagacaa aaaagttgaa aaagatcgta aataattgaa
    32761 cttctttttc acaaaatggt aagaatggag aatttcacgc atttgctctc aactattctt
    32821 tcttaagcct caaatccgtt tttatctgat acgtctttga accaatgacc cgattttttg
    32881 attgttcttt ttccatcttg tgctaagtct actgaaataa agccatatcg attcttatat
    32941 gcattggtcc acgaccagtt atccatacat gtccacatat gatagcctaa acaattggca
    33001 ccttcttgaa tggcttgatg gacatatttc agatgagatt ggacaaattc gatccgataa
    33061 tcatcgtgga tcatgccatc ttctttgata aatcgtgctt cgttttctac acccataccg
    33121 ttttcagaaa taaagcagtc gatatttcca taatgatctc gtacattgat caaaatatca
    33181 tagattcctt gttcatagat ctcccatccg cgataaggat tcattttacg accaggcatt
    33241 tcataattgt cgaaataatc ctcaggcatt ggaccgtttt tagtatcgat tggtgtttct
    33301 tttgctttta cccggcgtgg ctggtaataa ttgataccta aaagatctac tgtattttct
    33361 ttgataaccg tcaaatcctc tgcatggatc acaggcatga gatcaagttc ttttactatc
    33421 tcaatcaatt cacttggaaa ctcgcctttt acagctggat caaggaatga acgattgaag
    33481 aacgcatctg ctatttgcgc tgctttaaca tcttcagggt ttgattcgtc tcttggatag
    33541 cttggtgtca gattcaagat gataccaata acaccacctt gctccatttc atggtaagat
    33601 ttgatggcta acgctgaagc caacgcttca tgatagccaa cttggatcgc ttttttcata
    33661 tctgactctt ctggataatg ccatcgatat aaataacctg cttcgactgg aacgattggt
    33721 tcattatgag taaaccattt ttttactttc gaaccaaaca attcaaaaca acttttggca
    33781 tatttttcat aggcttcaac cgtttctcgg ttcaaccatc cgcctttttc ttgcagatac
    33841 atcggcatat cgaaatgata cagattcaca aagggttcga taccgttttc aatcaactca
    33901 tcaaaataac gatgatagaa acgaacggct tcttcgttga ttgttccaga ttcatcaggt
    33961 atcaaacggc tccattggat agatgttcga aatgaattat gcccagtctg tttcatcaac
    34021 tgtacatcct cttgatactt cgtataaact tgcgaggttt tttcaggtcc tacttgatca
    34081 aagaactttt gcggttcttt tttgtaccaa taatcaaaaa tgttctctcc ttttccatcg
    34141 cctgaaatcc ttccttctgt ttgtgggcca cttgctgcag aaccccacca aaaatttgct
    34201 ggaaattgat attttttcat caaagcacct ctttttcttt tttagttcgt tttagttgat
    34261 tccatgtatg atattttcca tcacaatcac tttttcattt tcttcctctt cttttttgat
    34321 tcgttttaac agctgttctg ctgctcgttc cccgatttta tctgttggtg gcgtgatcgt
    34381 gattttcgga aggatactca gatcgttttt atctaaataa tcatagtgac tgaacgcaat
    34441 atccttcccg attctgacac cttcttgatt gaaacattcg atggcaccaa tcgacatatt
    34501 gtaattgctg acaaaaatag cagttgcccc tttttctaat aatgcttttg ccccgtaata
    34561 gccgcttttt ctagaatagt ctcctgtaaa gacctctaat tggatcgatg gatcattttg
    34621 gaccgcttct ttggctcctg ctaaacgttc tcttgctacg tagtctgttt gagtggcagc
    34681 aataaatccg atttttttat gtccttgttc accatgatga gcgatcactt ttgctacact
    34741 ttctcgatca ttcactaata ttgtatcaat acctgctggt ccttctggac aattcaatgc
    34801 gataactgga atactcattt ttgtgagcat ttcaaatcca gaccactctt cttcggataa
    34861 gaagacaatc agcccatcga tcacatgctt catcaggtat tcaatttttt gttcaatttt
    34921 ttctggctga tcggcaaacc cgcttaataa taagctatac ccattttctt cagctactct
    34981 ttcgatccct gagacaactt ccgacccaaa acggctggaa atcccgttta tcaaaagtcc
    35041 aatagagaag cttcgattat tcttcaaccc tttagcaatg atgttttctt tatagtccaa
    35101 cgctgaaatc gcttctgcta tttttttcat gttcttttcc ttcagttgct gaccattcaa
    35161 gtaacgggaa actgttccca ctgaaacacc agccatctta gccacatcac ggatcgatgc
    35221 catacattct cactcctgcc agttacattt gatttctctt tcttgttgtt cgattatgat
    35281 gaccgcattc tttgcatagt gttgcttgtc atcaatatcc atataactaa agtcttcagt
    35341 agagcaactt cctctcacta ttaattatac ttaaacaagt atttgaaccg gttcattttt
    35401 aatatagcat ttttttaaaa gattgcaaca cttttttaaa acgctttctg aaagtggcgg
    35461 attttttgcc tttttaaaat tttattctaa aatttactct atttgtgggt gttttgtgcg
    35521 tttttctctt ccatccgttt gaagttttac attccaatgg tataatttct ttatcctatc
    35581 aaatggggga agtattttga agaaattaat cgtactattt attttgtctg cgggactttt
    35641 gacattgact ggctgtgatt tgtttggcgg ctctgaccaa tcctcggacg aaacaaccga
    35701 ttcttctgtc ttaaaagcgg aaaaagcaga taccaccaac agtgtatata cgctaaaagt
    35761 acgtaaagat atggatttat ccgcgggaca gttgacattt acagataaaa aaatggaatg
    35821 gacacgcacg tatcagacac aaggaactga ttcttctgaa tcatctgaag aaaaaacagt
    35881 gctgacagat atcaagatca ctaccaaaga tgacgaatat attatttcag gaaaagaaaa
    35941 taaaaaagaa acaacagtca tatttacaaa aatcggtaat taccgtttga aggatgagga
    36001 tggcaacatc tacagtctat agtgccgtct gttggatctt cttttgtttc tccccttctt
    36061 ttctgctata gtaaagaagc aagatgattg taaaggagac gaaatggatg atcgatagtc
    36121 ctgattattg tacatgtaat aacaacaaaa atatccatac agaatttaat gaccaaggaa
    36181 gcttgagcat atgcgacaac tgtcacaaac tagtcaaaga ttcatttaaa acatttgaat
    36241 cttatcctgt tcaaaattat gtaaaaagta gaagaagata aacaaatcca agcaaaactc
    36301 actatggctg atttttatga acttcaaact cagcttcttc ctttgatcaa gcttcatcag
    36361 taaaacaaac agtggatcat ttcagtttac aaatcaaaga gtaaagattt atttatttca
    36421 aatatttgaa tataaaataa ccaaacgata gaaaggctta ttctacatca cgtagtaatt
    36481 tttctttccc gtcgtttggt tttttatttt tagcctaatt cttgaccttt tgcatgctgg
    36541 ttatccgtaa aatattttcc agcaggcttt ccttccaatt cagctaattt ttcttggatc
    36601 tgacggatca ccttttttgc atcttctgga ttttctacaa aatcgaattt atccccatcc
    36661 acttgtattt taggacatac atcaaaatct tcataccatt gattatagcg ggtgttcaac
    36721 gtttcataat aggtatataa cgtctcatcg ctttctagct gttcatagtc acgcccacgt
    36781 tttttgatac gttctagcat cgtgtccaaa gaaactttga tatggaccat caaatcaggc
    36841 cgtttttttg gagcaacatc atctaactcg tttagcatcg tatctaataa gctatcgtac
    36901 tgctgtactt cgatatccgt tacccgacct aaatcagcgt ttagatggaa caacaggcta
    36961 tcttcataga tggatcggtc tagcacattg tcatcgttca ctaaagcatt tttcattgct
    37021 aaaaatcgct tgttcaaaaa gaagatttgc aataaaaaag cgtactgctc aggatttgaa
    37081 taaaagagcg gcaatacttc gttatcttcg acattttcat aaaatggttt agaatcaatc
    37141 tcttttgcaa gcatatccgt caaactgctc ttacctgcac caatcgttcc tgaaatcaaa
    37201 atcactgaca tacaaccctt cctcttctat aagtactcgc ctacgtaagt ttcaacaggc
    37261 aatgtttcaa aatcatattc acgtacagct tggtctgatt ttaaatacgt atggagactt
    37321 tgtgtaatca tcaagttcgt aggtaccgtc tcttcttgta caatgatcat cggttttttc
    37381 aacatagttg ccacacctaa ttcgtaagcc gttcctggat cgattgtttg atgctcaaag
    37441 tcaaggatag caacaacaaa ttctgcattt gtcagttctt ccatatcttt ttcgtatact
    37501 tcctgcgccc atccagcgct gaatagttca taattgcttt cttggtgatg acgtggagag
    37561 tagaaacttg tgactgtttt gttttcctcc aacgcttttt cgatacgact tactcgatcg
    37621 atttgctcct ctgaaaaaaa tggtcctgct aaatatattt gactcattat tttgcctcct
    37681 tcaataataa ctgattttta atagatactg ctcgtaattc attgaccagt tccaatggat
    37741 tgttggaaaa gattacttga tccaaccatt ttttatccat aaaatcctct tttgcaaaag
    37801 tttctaataa ttgttgcaag ccgctgtaat aaccgccaat attcaatatt ccgattggct
    37861 tgtcgggcgt cagaccgatt gctatccaag aaagaagttg tgagaattcc tccaatgtac
    37921 ctagacctcc agggaaaacg aggacagcat ctcccttttc aaataataat acctttcttt
    37981 catcgatcgt ttctgtagga ataaatgtag taacatcttt tccaggcaat tcagtttcaa
    38041 aaagcccatt cggatagata cctgtgactt ttccgttatt ttccagtact gttttactaa
    38101 tcactcccat aatcccggag tatgaaccac cgtaaaccag ttcgatttct tcttgtgcca
    38161 tgtattttcc taacgtttgc gcaataaatt tgtaactttc tttattacca aaacgggaac
    38221 cgcaaaaaac agtaagtttc ataaaaaaac actccctaac tcaaaagtct atcttcattc
    38281 tacacgtgtt tttttaaaat aaaagactag attttgtagt tttttagtgg tatatag
//
LOCUS       DAZUKZ010000018        37297 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000018 DAZUKZ010000000
VERSION     DAZUKZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.018"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..891
                     /locus_tag="ACG8EF_000849"
     CDS             <1..891
                     /locus_tag="ACG8EF_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFS4946196.1"
                     /translation="LTTGEDGKLAIDGLAPGAYQLVETQAPTGYELDATPIEFEIERS
                     QTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKL
                     AIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKI
                     DDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYEL
                     DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFIL
                     SILGVLLVFISIKFRKKRNI"
     gene            complement(1244..2092)
                     /locus_tag="ACG8EF_000850"
                     /pseudo
     CDS             complement(1244..2092)
                     /locus_tag="ACG8EF_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2245..3357
                     /locus_tag="ACG8EF_000851"
     CDS             2245..3357
                     /locus_tag="ACG8EF_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4946197.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3435..3728)
                     /locus_tag="ACG8EF_000852"
     CDS             complement(3435..3728)
                     /locus_tag="ACG8EF_000852"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFS4946198.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3896..4210
                     /locus_tag="ACG8EF_000853"
     CDS             3896..4210
                     /locus_tag="ACG8EF_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFS4946199.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4231..6210
                     /locus_tag="ACG8EF_000854"
     CDS             4231..6210
                     /locus_tag="ACG8EF_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4946200.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6223..6549
                     /locus_tag="ACG8EF_000855"
     CDS             6223..6549
                     /locus_tag="ACG8EF_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFS4946201.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6565..7083
                     /locus_tag="ACG8EF_000856"
     CDS             6565..7083
                     /locus_tag="ACG8EF_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946202.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7083..8435
                     /gene="celB"
                     /locus_tag="ACG8EF_000857"
     CDS             7083..8435
                     /gene="celB"
                     /locus_tag="ACG8EF_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS4946203.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8519..10024)
                     /locus_tag="ACG8EF_000858"
     CDS             complement(8519..10024)
                     /locus_tag="ACG8EF_000858"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFS4946204.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10116..10706)
                     /locus_tag="ACG8EF_000859"
     CDS             complement(10116..10706)
                     /locus_tag="ACG8EF_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4946205.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10885..12531
                     /locus_tag="ACG8EF_000860"
     CDS             10885..12531
                     /locus_tag="ACG8EF_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFS4946206.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12580..13299
                     /locus_tag="ACG8EF_000861"
     CDS             12580..13299
                     /locus_tag="ACG8EF_000861"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS4946207.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13778..14725
                     /locus_tag="ACG8EF_000862"
     CDS             13778..14725
                     /locus_tag="ACG8EF_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4946208.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14737..15657
                     /locus_tag="ACG8EF_000863"
     CDS             14737..15657
                     /locus_tag="ACG8EF_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4946209.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15698..17155
                     /locus_tag="ACG8EF_000864"
     CDS             15698..17155
                     /locus_tag="ACG8EF_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4946210.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17500..18114
                     /locus_tag="ACG8EF_000865"
     CDS             17500..18114
                     /locus_tag="ACG8EF_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4946211.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18107..19831
                     /locus_tag="ACG8EF_000866"
     CDS             18107..19831
                     /locus_tag="ACG8EF_000866"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4946212.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19828..21279
                     /locus_tag="ACG8EF_000867"
     CDS             19828..21279
                     /locus_tag="ACG8EF_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4946213.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21752..23905)
                     /locus_tag="ACG8EF_000868"
     CDS             complement(21752..23905)
                     /locus_tag="ACG8EF_000868"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFS4946214.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23907..24953)
                     /locus_tag="ACG8EF_000869"
     CDS             complement(23907..24953)
                     /locus_tag="ACG8EF_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4946215.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25103..26392
                     /locus_tag="ACG8EF_000870"
     CDS             25103..26392
                     /locus_tag="ACG8EF_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFS4946216.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26405..29107
                     /locus_tag="ACG8EF_000871"
     CDS             26405..29107
                     /locus_tag="ACG8EF_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS4946217.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29092..29949
                     /locus_tag="ACG8EF_000872"
     CDS             29092..29949
                     /locus_tag="ACG8EF_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4946218.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30020..30877)
                     /locus_tag="ACG8EF_000873"
     CDS             complement(30020..30877)
                     /locus_tag="ACG8EF_000873"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4946219.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            31088..33259
                     /locus_tag="ACG8EF_000874"
     CDS             31088..33259
                     /locus_tag="ACG8EF_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS4946220.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33327..33668
                     /locus_tag="ACG8EF_000875"
     CDS             33327..33668
                     /locus_tag="ACG8EF_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4946221.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33944..35698)
                     /locus_tag="ACG8EF_000876"
     CDS             complement(33944..35698)
                     /locus_tag="ACG8EF_000876"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4946222.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35709..36545)
                     /locus_tag="ACG8EF_000877"
     CDS             complement(35709..36545)
                     /locus_tag="ACG8EF_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4946223.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36547..37230)
                     /locus_tag="ACG8EF_000878"
     CDS             complement(36547..37230)
                     /locus_tag="ACG8EF_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFS4946224.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12035 a         6560 c         7654 g        11048 t
ORIGIN      
        1 ctaactacag gagaagatgg aaaactggca attgacggtt tagcaccagg agcatatcag
       61 ctggtagaaa ctcaagcgcc aacggggtat gaattagatg cgacaccgat cgagtttgag
      121 atcgaaagaa gtcagacagc ggtggtagaa ttgacgaaag aaaaccgact tacaccaggc
      181 ggtgtcgtgt tgacaaagat cgatgaccaa agcggggaag tcctgcaagg agctgttttt
      241 gagttacaag atgccaatgg aaccgttctg cagtcaggtc taaccacagg agcagatgga
      301 aaactggcga ttgacggctt agcaccagga gtgtatcagc tggtagaaac ccaagcgcca
      361 acggggtatg aattggatgc gacaccaatc gagtttgaga tcgaaagaag tcagacagcg
      421 gtggtagaat tgacgaaaga aaatcgactt acaccaggcg gtgtcgtgtt gacaaagatc
      481 gatgaccaaa gcggagaagt cctgcaagga gctgtttttg agttacaaga tgccaatgga
      541 accgttctgc agtcaggtct aaccacagga gcagatggaa aactggcgat agatggctta
      601 gcaccaggag catatcagct ggtagaaact caagcgccaa cggggtatga attggatgcg
      661 acaccagtaa ctttcaagat cgaaaaagaa caagaagtgg cgatcttttt aacaaaagag
      721 aatagaaaag ttgctgatag ctcagatata agaggaaaca atccaacaag cagtggaaat
      781 aagcatcttc caaaaactgg agaaaccatc attagccagt ttatattgtc aatattgggt
      841 gtgctacttg ttttcatcag tataaaattt agaaagaaac gaaatatatg agctaaaata
      901 aaaaattgaa taagcatagt ttttaaaatc aactagtacc catcaattga atggaaacta
      961 ccgattttag aaggggtata caaacaaata ttagtatcgg agtagagaag gttgaaccca
     1021 accagtaaat aagtaaagac aattttgtgc tgcgttgaga agtgaaaaat aaacctttgt
     1081 agtaatacta gttaataaaa aatatacaaa aacactgcga aaatcattct tgagtttcgc
     1141 agtgtttttt gcctaatgac tcaagtttat tgggtaatac tttgattttc tttctacaaa
     1201 taatcagcga tcagttgtaa ttttttctct agttttaatg gctctttgtc aataaggaga
     1261 aaaacaagtc gggtcttttg tctcggaaag cttcaaccaa ctcgtgaata aaggttacgc
     1321 ctccttgaaa ggcgtggaaa aaccaagtaa tcgatcaatc accaatttcg catttggtag
     1381 aacatgtttg gtgagctgaa agtaggtggc gttcatgtct gtaactaaga attctatttc
     1441 tttaggatta gtgcagctaa ggaaatagtt tgttaatcga ggaatttttt gtgtaggtaa
     1501 attttctcga ttaatttttc tgtctcgcca tccgcggaaa taaaactcag tttatcctca
     1561 acggaaacgt gagaacgaaa ttcacaaccc tcaacactca agacagaatt cttttggaag
     1621 actttggtaa atagcttctc aactctttca atgtgcgaat aacggttgtc aaagatacct
     1681 aacaactttt cgaaataaaa aataacgaca ctttttcagt cagtaaagaa gtgattttat
     1741 gtctgacatg atttgaaatc gagtgccttg gacggataaa ataactttga gcgttccaat
     1801 ggcttcgaca gtttttacag gtatagcgct gcttttttag gcgtataacc attggcatat
     1861 gattatatta ttcaaagtgg acaatggttt ccttcttctt gtttttcacc accattaatt
     1921 tcctatttcc atctaccaca gcagaaccac aatttctaca gacacatgga gcagggaaga
     1981 gaactgcagc aacgatcaat gtttttttct tctgtaaggt ttcgtaagag acctctgtaa
     2041 tcatcaaatt tttgtctatt gatctcagaa tttttttgat agaaccattc ataaagcata
     2101 tcgttctctc agttgtttat tttgtggtga tttaatcata caagaaaacg atatgttttt
     2161 ttataaaaac atgaatagga aaaatagttg acaagaaaat gacaacgcca tatgatgttc
     2221 catagtattt acaacctgtc atagatgact gtaaaaaata aaagtaaaaa gaaaggacga
     2281 caacaggtgg atttttatca gtctctacaa ctagatccgt ttattttaaa acaaaaaata
     2341 cgagaagcag cttctaaaaa agaaaagtgc atgtatattt gttcattgtt tttacgatca
     2401 ttattcatcg tattgtttgc catttgtttt atcattataa ttacaacatt atttgaatcg
     2461 aagcataaac catacgctgt ggttttattc tgtatgttga tgagtattcg ttttgttgac
     2521 tttggttata agatttccca ttcgatcata ggtttagcaa tcgtcatgtt ctcactatta
     2581 gtagcaccgt atgtccagct gatcaaatgg tcagttatgg gcatgttgat tcattttatt
     2641 ttactaagca gcatattaat ggcaaccgct agtgatccca aaatggggaa tgcttctttg
     2701 tacggctttt catatctctt tattgtttat tcgttaccta aagattcgtt gaacaagaat
     2761 ttctttacac aaacaagtag cttattattt ttgttttttt gttggttttc agttctcctt
     2821 tacagaaaac atcgagaaaa gaacaaagac aaaagcttgt ttaaagagat ttttttgaaa
     2881 ggcatgtatt cgcaggaaaa aatttggatg ctcatctatg catttggcat tagtcttttg
     2941 atcgttgcag gagagtatgt cccttttcag cgattgatgt gggcagggtt tgcattttct
     3001 tctattgtat ctagctatgg attgatgtcg attggtttca aagaacgagc agtagaccga
     3061 atcattggct cattgattgg atgtgtttta tttattggta tcagccaatt tattcctttt
     3121 aactgggtcg gtatattagg tggtctagct ttaggtgttt gttctaccta tagatataaa
     3181 acagttttta attcttttgg tgctttggca atcactgcca gtttattcgg ggttccagga
     3241 gcagtaacga tccgcatttt tgagaatatc cttggggtat gtttaggaat catgtatatt
     3301 ggggtgactg aaatacttat ccgaaaaata agagaaaaac acggtttgaa tcattagatg
     3361 agatcgaata aaaaataagg aaaaagtgaa gatacacatg gatgataaat tgctatcatt
     3421 ccatgtgtat ctttttacgg taaatcagtg aaacgttcga ctttcatttt taatttatac
     3481 tttttacgga gttcgtttat ttgagccatc atttcggatt tgtgatgctt ttcgattgct
     3541 tcttcatctt tccaacggtc gatcagtaaa agagattctg gatcttctag tggaaagtaa
     3601 tagtcatatt tctcgtttcc ttcttctgca cgaatcgctt tgacaatgtt tctttcgacc
     3661 atttcttctg caaattttct ggcttgtcca tttgtacttg tatacaaaat attcactgtg
     3721 atactcacta aatcccctcc tgaaaaaatt ctatcactag tatatcacgg tgccagaaat
     3781 tattattcaa gatatgatac acattttttc cgcaatgcta cggaaatata tattttgaat
     3841 agaaaacgct tacaagatat gcttggttta taaaaataaa aggagtgatt gatttatgaa
     3901 aaaagcattg attatttgtg cggcaggaat gtcatcctcg atgatggcct caaagacaac
     3961 ggatttcttt aagcaaaaaa gagaagaggt actcgtagat gcagtttcag ctacagaagg
     4021 cgataatatg attaagaata gtgattttga tttatttctg atcagcccgc aaacgacgat
     4081 gtttttggat aaattcaaaa aattaggaga atctgttgga aagcctgttg ttagtatccc
     4141 tttccaagcg tatgtcccaa tccctacagg catccagaag ttagcagagg tgatcgaaga
     4201 gaacatatga tggaatagga gtggatgata atgaaacaga aggaaaaatt gctgttgaga
     4261 tatttgattg atcacaagaa ggagtatgtc accagtcaac gtttagcttc tgaattgtta
     4321 ttatctgatc ggacaatccg taattacttg cagcggatca aagagttagt agaaaaaaac
     4381 ggaggtaaga tcattgctaa accaggttat ggctaccaac tgcatatttt acaaagactg
     4441 acatttgact tgtttttgag tcggcaagaa atagtacctg gatatgctag agaaccgcaa
     4501 ggattttatg agtccgaaga tcgacaaaag tatatattga accaattgct tttggaagat
     4561 gccattcttt ttatggatga tttggcagaa gagctttata tcagtcgttc cagtttgaca
     4621 aaggacatgc aagaaatcaa ggagcgattg attccatatt cactaaaaat cgtttctaag
     4681 catggtcaag ggacatggat cgaaggggaa gaaagaaatc gacggcattt tatcatggat
     4741 actttttttg gaaaccatta tggaaattca ttgaaagaat atttagggca aagccgtttt
     4801 ttcccagaca tcagttttga agagctagtc attatcattc tcgatgaaac aagagaagct
     4861 aagttaaaag tttcggattt tatcatacag aatctgactt tacacttggc tttggggatc
     4921 aaacggctcc gagaagggtt tgaaatcaag gaattaggca tcgaacaaga agtttctaag
     4981 cgagtagagt atcaagtggc gcagcggatc gttcaacgta ttgaggtaat cgcaggtgta
     5041 tcgtttcccg aagaagaaat tgcttatttg acattacatc tcatggctaa gtcgaatcac
     5101 aaagaaaatc aaaatgatca ggaactatca aaggaattag cgaaagttct gaaaagattg
     5161 tctcaggaat ttcatttttc attagtagag gatctccagc ttttcaatgg actactcgat
     5221 catttgtcac cgatgatgat tcgattgagc cgaggtattc gcattgagaa cccattgact
     5281 gaggagataa agaaagaaaa tcccaaagtc tttgacgcag taaaaaggca tttcagcaac
     5341 atgccggctt tgaaaaacta taccatcaat gaagatgaat gggcctattt ggctttgcat
     5401 ctcatggcgg cattggaaaa agaaagagct gcgcataaac tacatgcatt gattatctgt
     5461 gcaacgggtt acggcagtgc ccaactatta aagaatcgcg tagtcagtga attcggtaaa
     5521 aatattacag tggtaagtgt aaaaggctat tatgagatca atgaaacaac tttaaaagga
     5581 atcgacctga ttatttcttc tattgatctt tcgacaatgt ttttcaaaat tccagtttta
     5641 catgtcagta tttttttgaa tgaagatgat gttcgtaaaa tacgaaaggt agttggagaa
     5701 tctataccct atcattcctc agatagaccg ataccagcat tttctctaca gcaaaaaaag
     5761 cagatttaca ccgaacagtt gtctgaaaaa tttttcaaga cttatacgta cgaaccgaaa
     5821 aaggaagaaa ttcttcatga tttgctgcaa aagctgagca taaatgaaga agaaggatat
     5881 ataagagaaa tgcgaaagca gatccagcaa cgtgaaaata tgggacaaat catattcagt
     5941 gatacagtgg cagtcccaca tcccgctgtt ccagttggaa tcgaaacaaa ggtagccgtt
     6001 gcattgattc cttctgggat gcagtgggat caatatcaag aaattcattt tgttttttta
     6061 gtttcgcctt cttatgtcga aaatgaagga attacggtcg tgactaaagc aatcgttagg
     6121 ttagttgacc agctaaaagt acagcaggaa atcttagctg aacctatttt tgagaatttc
     6181 agcagtaaat ttatcaaact gatcgaataa ggaggaaaac agatggctga aaaaataaac
     6241 agtgaaacac tacaagtgac ttcttttgat atcattttcc atagcggaaa tgcacggaca
     6301 ctgatacatg aagcatttcg ctcgatgcgt aaagagatgt ttgaagaagt agaagagaag
     6361 ttgcgacaag caaatgaaga gattcttcat gcgcatcagt cacaaactca acttctgaaa
     6421 gaatatgcca gcgggacaaa aatagaaatg gaaattatca tggtccatgc acaagatcat
     6481 ctgatgacga cgatgacatt attggaagtt gcacaggaaa tgaattattt gtatcaaaaa
     6541 aataaataga aaggaaggat cattgtgcag gatacagtaa aaaggaaaat cgaattagaa
     6601 aaaaagcaac ttagtttgaa aaacatgtat ttcaatcgtt atctaatcgt acgttacctg
     6661 actgcctttt tctttttcat gaacatgcaa tggatgattc tgcttctatc agctaaaagt
     6721 ttaggaagta gtttaccgat gattctgatt ttagctattt tgcctgcagt aggagaacaa
     6781 gtaaagcttt atcgaaaaca ccaaacgaac gttccatgga caaagtggta ttttttgttc
     6841 cagagtgtct ttaatttatt agtgattcca gtgttgttta cctcaggatt tacattcttg
     6901 tatcctttta tggcaaataa caatggagga cagttatttg ttttcattct tgttgtaagc
     6961 ggtatttttg ccagtgcatt tatacagtat cgtttaaaga aaatttcact caaccaagac
     7021 caacagtata tccgtattaa acaatatgaa aaagcgttat atttaggaaa ggagaataac
     7081 taatgtttga ttttttgcag aaatacctga tgggacctat ggggaaaatt gcgcaactta
     7141 aaatcgttcg ggctgtcatg gcagcgggga tggcttctat tcctttcacg attgtaggct
     7201 cgatgttttt ggttttaaat attttgccat taccgttccc atttttagaa ggatttttta
     7261 atgcaacttt tttcaaagtt agtgacttgt acatgatcgt caatacaatg acgatgggga
     7321 ttttatctat ttactttgcc attgtgtttg cttatgaatt aacgtcaatt gaacgagatg
     7381 aacaaaaatt aaatgttaat ccgctcacgg gggcattact atctgttttt gcattcttta
     7441 tgtgtatccc tgaattgatc atgactgatg gtaaaatcag cttgattgct tctatcaatg
     7501 agacagaaac tgtagtaaat ggtattagga tgggctcttt tgtcgaacgt ttaggaactt
     7561 caggaatctt tacagctatt attatgtcga ttattgcggt cgaattgtat tgtatgtgtg
     7621 tcaagagaaa ttgggtcata aaaatgccag atgttgttcc gccaggagtt tctcgttctt
     7681 tcactgctct gatcccaaca tttgtcattg cgtttgtggt gatgatcatc aatggattat
     7741 tagttgcact aggaacggat atcttcaaaa tgattgctat cccatttggg tttgttacgc
     7801 agttgacgaa tacatggctt ggtgtcatgg tgatttattt cttgatccat gcattgtgga
     7861 tcgtagggat ccatggagcg aatatcatca cttctttctt gaccccgatc gtattagcta
     7921 atatggcagc taacgcacaa ggcgcaaatt atccgttggc aggagaattc aacaattctt
     7981 atgtaaccgt tggtggatca ggtgcgactt taggattgat catctttatt gcattcctcg
     8041 cgaaatctga tcagctgaaa gtgttaggaa aagcctcact tgtaccagga ttattcaata
     8101 tcaatgaacc aatcattttc gggatgccaa tcgtctacaa tccctattta gcggttccgt
     8161 ttttcttagc tccgatggct tctgctacac tcgcttattt tgccattcgg ctggaaatcg
     8221 tcaaaccgat gcttgcacag atgccgtggc cttcaccagt cgggatcgga gcatttgttg
     8281 gtagtggtgg tgattggaaa gctgctgtgt tagcagtcat ttgtgcaatc atcgcattca
     8341 ttatctggtt cccatttatc aaattctatg ataataaatt attaaatgaa gaaagagaaa
     8401 aggcagcaga attagctagt gaaaagttaa aatgatttct aagaaaaaag gaactgtgac
     8461 aaaaaatcac agtccctttt tttcttagaa atgggaaacc actagtatat aaataccatt
     8521 attcttcaat caaccgcttc acgattgcat aatccccttc ataaggttca tctacaccat
     8581 tgattttttg atagggatcc acgccttttc ccgcaataac gactgaatct tgcggagcgc
     8641 tttctgctaa agcgcgtcga atcgcttcag gacgatccat ttcataatga acgatcaaat
     8701 ctggattagt aattgcttca ttgatttctt ccgcgatttt tttcggatct tcaaatgctg
     8761 ggtcatctgc cgtcaaaaag gcgatgtcag ctgtttctga tagcactttt ccaaaatcat
     8821 gtcgacggga gattgctttg tttcctgggc tgcctaatac aacaattaca cggccatctg
     8881 gatgttcatt tttggcaaat ttcagcaatg ttttcaagct tagataatta tgtgcatagt
     8941 ccacatagac atgcgctcca ttggattgga tcaattgatc cattcggcct ggtacacgag
     9001 cttcttttaa tccgtcttgt gcatcttgac tggaggcacc tgttagtgct gcggcaatga
     9061 tcgcactggc tgcattttct tggttgaatc ctccagccag caaaatatca taagcagtat
     9121 cgcagatagc taagcgatct tcatgagaag ataaagtaaa accgtgagta cctttatctg
     9181 aacggatcac ttgataattg ctttcttccg aacgaccata agtgatcgtt ggaatctgga
     9241 atagatcggc tgtttcccta agtaaatcaa aatagtcact ttcatgattt aaaacaactg
     9301 tttttgaatt caagatcaat tgtcgcttgc aatagaaata gtcatcaaat gtcggatgtt
     9361 cgatcggact gatatggtca ggcgagatat tcaagaagat tccgacatct aaagtcaagc
     9421 cgtatacgcg ttgtgttttg taagcttgtg aagaaacttc catcactaga tgagtcatac
     9481 cattttcaac tgcttctgac atcatacgat ataaatctaa tgactcaggc gttgtcaaat
     9541 gggatttgaa atatgttttg ccatctaacg ttgtattcat agtagaaagc aatgctgttt
     9601 ttttatttgt tgtgtgatcc aagattgctt ttgtaaagta cgcagcagtt gttttacctt
     9661 ttgttccagt gaatccgatc actttcaatt tattttgcgg atagtcgtaa aaagccatag
     9721 caagaagcgc cattgcttta cggatatccg ttacgatgat tcctactgct gaattttcgt
     9781 atggttgttc tgatacataa tattgaatac cctgttcgat tgctgaatcc aaatattctt
     9841 ctttgaaatt cattccttta cagaaaaaca aagtagaagc atcagctttt cttgaatcat
     9901 aacttaactg aagaaattca gctttttctg gtacatcaag atgccagcct cttgttgaaa
     9961 caaattcttt taatagattt tcttttaata gtaaatgacg gatgtcttct aaagagaagc
    10021 tcattgattg atccctcgct ttattcaatt tcttggttta ttatagcaca ctctttttaa
    10081 tatatcgttt gtttccgcta tgacaaaatc cagctttatt caaattttct tgacacaggg
    10141 gacaagcgga tgagtatttt cataaacaat gaactgactg taatagatag gagaacgaat
    10201 agtgtctcca acaatccaaa aacataataa ttcaaaaaga cgataagact attggtaagt
    10261 aaaagtcctt gcggcattgg aagccgaaaa aatttgtgga aaataatcgg cggtatcgtt
    10321 ataccgccat ttgaggcccc gactgtgtac aatgtgtaaa ccccaagact gaacaaaaga
    10381 cttcctgaaa tcagtgaaaa gaaatgtgag gagatcattt ccacttttgg aacaatctct
    10441 aaaacaacag gaaaaagcag acttcccgct acagtcttaa aaaatactga ttttcctaat
    10501 aggacagcgg ctagaagcag catcataaga ttgatgctcc aaacagtaac tgagcgttgg
    10561 atagaaaagg ccgtttcaac taaatgtccg atggcgccca ctccggcgga agctatatcg
    10621 tgagggagca agaaccaatt caatccaact gtgataaaga aaagacctaa aaatataacg
    10681 ggtagttttt ttatttcttg tgtcattgat atctcctata aatgttagcc ataaaaggct
    10741 gtatatttct ttactttacc aaaaaaacga aaccgaattc aaaaaaacaa ttgttcttat
    10801 aaaagttata taaaatcgat tgccagtttc tgttttacca attaaaatga tagtaatgtt
    10861 ttgtagaaag ttggaatctg accgatggat aatcaaagaa aaccagcatt agatcaatta
    10921 acaccgcaag aaaaaatggc acgaggctct gcttggatga ccgccagtaa tatcatttca
    10981 agattgctag gcgctgtcta tatcattccc tggtatgctt ggatgggcga aaatgctaag
    11041 gctgccaatg gattattcaa tatgggatac aatatatatg cattgtttct actgatctct
    11101 acagcaggaa tacctgctgc gattgcaaaa caaactgcac gctataattc attgaatgaa
    11161 tatggcactt cacgacgatt atttatccgt gcattacaga tgatgggcgg gttaggcctg
    11221 ttgtttgctt tgttcatgta catcgcctct ccttggctag ctcatgcttc tggtggcgga
    11281 gaagaactga ttccaacgat gcgttctttg agtatcgctg tattggtttt tccttgtatg
    11341 agtgtgatac gagggtattt gcaaggcaat caagaaatga tgccttatgc gctgtcgcag
    11401 attgtagaac aggtggcacg tattttttat atgctattgg ctacattcat catcatgaaa
    11461 gtcatggatg gtgactatgt gacggcggtc acgcaatcaa ctttcgcggc atttatcggt
    11521 atgctagcaa gtattcttgt ccttttgtat tttttgaaga aacatcaagc gtatacatcg
    11581 gcgtttattc actacagcga aaacaaaata aatattgcaa cgaaggaatt gttgttagat
    11641 accgttaaag aagcgattcc gtttattatt gtgggttcag gggtaacgat cttcaaatta
    11701 gtcgatcagt tcacttttat gaagatcatg tctgcatcca ccgagtattc caatgcacag
    11761 ttactggatc ttttttcgat ttttagtgcc aatccagata agttgacgat ggtagttatt
    11821 gcattggcaa catcgatcgc gtctactggg ttaccgttga ttacagaagc tgttacgttg
    11881 aaagatcgag taggtctggc aaaattgaca agcggaaatt tacagttgtt ttcatttttt
    11941 atgtttccgg cgacatttgg tgttatgtta cttgcctatc cgctaaatac attattttat
    12001 acaccggatt ctctaggaag taatgtactg atccaagcaa gttttgtagg actcttccta
    12061 ggactatata tgctagtttc caacatgctg caaggaatgt ttgaaaacaa ggcggcaatt
    12121 aattatttgg ttattgggtt tctagtgaaa ctccttcttc aatacccagc cattcgttta
    12181 tttgaagtat acggcccttt attggcgaca atgatcggat ttgctgtgtc ttgtaccttg
    12241 atcttaaaga gaatccatga agtagcccgc tttaaaccaa gttttgtctg gcgcaggaca
    12301 ttgctgatct ttattcttac cttgattatg cttttggctt cgtggatcac aaagatgatt
    12361 tttggtgctt ttttaaacga agatagcaaa ttccagtctc tattgttgat tcttgtagta
    12421 gcaggtatag gggctttagt gtatggttat ttagctttga aaatccgctt ggcagaaaga
    12481 ctactaggac caggaatagc gcgtttacga cataaattaa aaataaaata agtgaatcag
    12541 aagagctaga ttgatgctct tttgaaatgg gggaaacgca tgcgtttaga taagttttta
    12601 gcagatatgg gacttggcag ccgcaaagaa gtcaaaggat taatcaaaaa aggaacgatc
    12661 gaagtcaatg gacaagtggt gaaaagcgat aaagcgcaag taaatgaatt cgaagatgcg
    12721 gttacttatt taggagaaat cattacgtat caaaaagatt tttactacat tttgcataaa
    12781 cctgcaggag tcatttctgc tactcaggat aatcatgatc aaaccgtaat cgatttattg
    12841 gaagaccagg attatcggga agatcttttt ccagtcggcc gattagataa agacacagag
    12901 ggcctcttga ttttaacgaa tgacggggtg tttgcccatc agttactatc acctaaaaaa
    12961 catgtggaga aagaatatct agctgaggtt agcggtataa tgacagaaga agatatccgt
    13021 ttatttgcag aaggactgat tattgatggc gatgagcaga cattgccggc aaaactccaa
    13081 attgatcaag tagatcaaga gacagaaact tcaattatcc gtctgatttt gcatgaagga
    13141 aagtttcacc aagttaagcg aatggtcaaa gcagtaggaa aagaagtctt atatttaaaa
    13201 agaatccgta tgggaaaatt ttggctgcct aaagatttga ataaagggca atatcgaccc
    13261 atgaccaaag aagagctaga acaagtgaga gaaaaataat tgaatggata gctaaaagaa
    13321 taagcagagt agttgtagcg ccatctgaaa agcagacttg tccgtctgac tttcggatgg
    13381 cttttttgtg aaatttcggc ttatttccgt agaagctact tctggaacac cgtttgttcg
    13441 gaataaggga ctgtgacaag acttttgtta cagtccccat tgttccttgt gactcagagt
    13501 tagatagtaa ggacacgatc tccctaatac tcaggccaaa acgctgaatt cacaacctct
    13561 gataaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc aaaatatgag
    13621 gaactatttt cccgtttttt tctctaatac tcaggccaaa acgctgattt cacaacctct
    13681 cctatacttt gtttcatttt ccctatactt tgtttattta ttctcattat tgaaagcgct
    13741 ataattacgt tgcgaatgaa tcgaaagggg tttttagatg aaaaaacgaa aggggttcaa
    13801 tcattttttt atgaatctat ggcgttataa agcacttgtc ctgatggctg taccgggtat
    13861 gctttggatg atttttttct tttacatacc tgtaatggcg aatgtcgtag cattcaaaaa
    13921 ttttcatatt tcagcagatg ggttcttggc tagcttgagg gaaagtcctt gggtgggttg
    13981 ggaaaatttc aaatttttat tttcttcgga tcaagcattt ctcatcacta aaaatacaat
    14041 tttatataat gtagcattta tccttttgaa tctgctgatc tcagtcgtat ttgcaatcat
    14101 tatgagcgag ttgagaaata aacgactcgt taaagtttat caaacgatgt ccttactacc
    14161 ttattttcta tcttgggtca ttatcagtta ctttgtttac gcttttttaa gtcctgacaa
    14221 aggaatattc aatcagtgga tcactggaca tggaggagaa gctgtcaatt ggtataatga
    14281 accgaaatat tggccgttta tcttggtgtt cattggtaca tggaaaggga ttggttataa
    14341 cagcattatt tactttgcat ctgttatggg aatcgatccg acttattatg aagctgctat
    14401 ggttgatgga gctagcaaat ggcagcagat caaacatgtg acgataccac aacttattcc
    14461 actaatgacg attttaacga ttttagccgt aggaaacatt ttccgagctg attttggcct
    14521 gttttataac attcctagga attccggtgc attatatgat gtgactcaag ttttggatac
    14581 gtatatctac aatggcctga catccacagg agattttggt atgacggctg ctgcaggact
    14641 ttaccaatca gttgtcggct ttgtcttgtt gatgatcaca aatacgatcg cccgacgttt
    14701 tgatagcgat tctgcattat tctaaggagg aaaaaaatga aaaaaaagaa agtggagaga
    14761 gtccaagtcc gctctttcaa taagacggct aattttttct ctaatataat gattgcactc
    14821 tttgctattt cctgtatttt gccatttata tttgttatcg ccatctcttt taccgatgag
    14881 tccatattgt cgactcacgg gtacagtttt tggccagaaa agttcagtat atttggttat
    14941 acgtacttgt tcgaccagat gcaggataaa attttccaag cgcttttcgt cactgtactt
    15001 gttactgttt taggtacact gatcaatagc acggctactt ctttatatgc ttatgcgatt
    15061 tcacggtcaa actttccatt ccgtcgtttt tttaccgttt tttgtttgat tacgatgttg
    15121 ttctcaccag ggatggtggc caattatctt gttatgacaa atcttcttca gttaaaagat
    15181 acgatttggg cattgatctt gccaatggct gtcagtccct tcaatattat tgtgatgcgc
    15241 acattcttta aacgttcggt ctctgattcg attattgaat cggcacggat cgatggagct
    15301 agcgaactgc gaatatttgt tcagatcgtt ttaccgttag ctgttccagg tattgcgaca
    15361 atcagtttat ttgccgcatt gggttactgg aatgactggt tcaatgcttt actatacatc
    15421 caagatgata agctagttcc actacagtat cttttgatga aaatccaaag taatattcaa
    15481 tatctgacac aaaattcagg agcaggaagc caattgacag gcggattagc ctcagttcca
    15541 ggagaatctg ccagaatggc gatcgtagtc atctcgacgt tgcctattgc gatcagctat
    15601 ccatttttcc agaagcattt tgtcaaaggt ctaacaatcg gcggagtaaa agaataaaga
    15661 aacaaaaata taaatcataa aattaggagg aagaaaaatg aagttatgga agaaactagc
    15721 ctttacaggt gtttcagcgt tgttgttggg aacattagca gcatgcggag gttctggttc
    15781 taaagaccag gcagaagcta gcaattcgga cactttgcag atgtatcaaa tcggagataa
    15841 accagataat ttcgatcaat tgatggaagt agcaaacaaa cgaatcgaaa aagagatcgg
    15901 tgtaaaagtc aatatcaatt acattggctg gggcgattac gaaaagaaaa tgaacgtcat
    15961 catttcttcc ggggaaaatt atgatatcgc ctttgcgaac aactatgttt ccaatgccca
    16021 aaaaggagcc tttgctgatt tgactgaatt agcgcctaag tacgctgaaa aagcttataa
    16081 tgatttagat gaagcatata ttaaaggaaa tctggtcaat ggaaaattat atgctttccc
    16141 agtcaatgga aatgtctttg ctcaacaagt acttactttt aacaaagctt tactagataa
    16201 atacgatctt tcaattgatg gaatccaatc ttacgctgat gcagaaaaag tcttgaagga
    16261 attccatgaa aaagaaccaa acacggcagc ttttgccatt ggacaaggct tcaaagtcca
    16321 agggaacttt gattatcctt tagggaatac gttaccattt gctattgatc tggatggaga
    16381 tgattcgaaa atcatcaacc aatacgataa tgaaacgtat atcggactgt tacgtacaat
    16441 gcatagctgg tacaaacaag gactcattcc aagtgacgct gcaacgagca ataaggatta
    16501 tccactagaa ggaaatactt ggctaatgcg tggagaaaca caaggaccgt atgattatgg
    16561 cgatacaatt ttgacaaatg cagcaaaaca agaacttgtc tcaaaagcaa ttactgttcc
    16621 actaaaatct acagctcaag cacaaatggc aaattttgtc gtatcaaata cttcaaaaaa
    16681 taaagaaaaa tcagtagaac ttctagggct gcttaacagt gatccagaat tattgaatgg
    16741 gcttgtttgg gggatcgagg gagaagcatg ggaaaaagtc gatggttcag atgataaaat
    16801 caaattgcta gatggttatc agccaaatac acatatgtct gcttggaata caggaaacaa
    16861 caagattcta tatacacaag agtctattac ggatgacatg attgccaaac gtgatcaatc
    16921 gattgctgat gcagaaactt caccgatttt aggattcagc ttcaacactg acagtgtcaa
    16981 aacagaatta agtaatattt ctaatgtcat gaatcaatat ttagatggat tgaatacagg
    17041 gactgttgat ccagatgaaa cattaccgaa attgaaagac gctttggaca aggctggcta
    17101 tgataaggta ttgaaagaaa tgcaaaaaca atatgatgag ttccgacaag aataataatg
    17161 aataaagaca aaggggttgt gacaaaagac gtgtcacaac ctcttgttcc gaataaacgg
    17221 tgttcaaaag ccagtttctc ggtattaagc taaatatatg taaaaatgtg gaaagccata
    17281 ttcacaaaat ttttcttact actcgaaact aagcggctat ttcacaaact cttggtaaac
    17341 ggtgttcaat cagctaaccc tcggtattcg ctcacaaaac gacaaaatat gaggaactat
    17401 ttttccgttt gtttctctaa taatcaggtc aaggtgctga ttgcccaacc tttttttgta
    17461 taatgagaaa gtataaactt aatgtttgga ggagaagata tgttagggaa ggttttggaa
    17521 gagttattta tccggatctg ggtcattata aaactaacct tgtatttctg gatatataca
    17581 tttgccggcg ggatcatctt tggattaggt gcggcatgga aaacagtcaa tgaactattt
    17641 tatttgtacg gattcgagta taaagaaatc acaataaaaa gaggctggaa tatatataaa
    17701 agaaattttt taagaggcaa tctgctattt agtttatttt tatctggtac tgctttattg
    17761 agttacaatt tatatttatc tgttcaaatc aaagggctca tttttttagc tattgatttt
    17821 attttacttt ttgccttgct ctgcctattt gctacttatc aatacagtat gattttagat
    17881 agtgaatata cgatctctgt tcctaatctg ctaaagctaa gttttatttc ggttttttcc
    17941 agcttttcgt cttttctaaa aataattatt ggtagcggta taattttagg cgtgacttgg
    18001 caattcaaag gattgatcct gtttggtgtc attggtcttt tgacggtttg gaatggtacg
    18061 atgacaacac attggcgtga agaattagat aaacagttgg aaagttatga ataagtttcg
    18121 gattctattt tcaaaaggaa ccttactaaa tcgtctgttg cggaaatacg cggttatcat
    18181 gatcggtgta acgatgactg ctaccgttat ttttagtatc catacttggg aacaaaatca
    18241 aaaacaggca gaaaacatga caagtgatgc cgttcaaagc acaagcagaa tgctgaatga
    18301 taaaacaacc ttgagtagga tcatcaaaaa ccagcttgta gggaattccg aaaagatcga
    18361 aaatgtgacg acctacttaa caaaacccat agatcagtat ctgatgtatg tatacgaaca
    18421 gcaaaacagt accgatgaat tagtttcttt cccaaatcaa atcaaagatc tctacgcaaa
    18481 ttatgaagaa ctaagtgcga tatatatcgt gttgaatcaa ctaccggagt attatgaatc
    18541 ttctagagaa aacaaaggtg gggaaataaa aaaggggaca ccaaaattac tagacgcctt
    18601 ttatatcaag ttgcctatca cacaaggagg agctgagtca ggaagtattt ttattggttt
    18661 tcaaaagaaa gaactagata tgattttaga aaatctaacg tcattcaatg gactctcttt
    18721 atacatgatt tctggaacga caaatcgact atacacattc catgataata aaatagctaa
    18781 agaaacgttt gaaagacaag aaatgatcat cacagagagt ctgaagaaaa attcacaatt
    18841 gccgattgac gatctggaaa aaaacaattt tgttcaacac caagaactgt ctgatgatta
    18901 tcgtatatta gctgtactag ataaaaaaag tgtaaaaatc gaaacgggaa aaaatcttgc
    18961 cccgctgatc attggtatca ttattttaga caatatactg cttatctttc tttataaaac
    19021 attcaaacga tattcacagc aagtatctat cgtcatgcag gccatggatc aaacagcagc
    19081 agggaattta gagacgcgga tcgacataac aaacacggaa tatgaactga gggaactttc
    19141 aatcggtatc aacgaaatgt tggatagcat caatcagttt gtagaagaca tctacaaatt
    19201 agaaatcaaa cagcaagacg cacatatgcg ggcgcttcaa gcacagatca atccacactt
    19261 tttatataat acattagaat atatccgaat gtatgcaatc agcgaaggaa gtgaagaatt
    19321 agcagatgtt gtgtatgcgt tttccgcttt gcttcgaaac aatacgaatc aagagaaaac
    19381 gatcacttta aaagaagaac tggacttttg cgaaaaatat gtctatttgt atcaaatgcg
    19441 ttatcctaat cgagtagcct atcattttat gatcgatcct gatttggaaa agatcgaagt
    19501 acctaaattt gtgatccagc cattagttga aaattatttt aaacacggta ttgattttac
    19561 tcgattcgat aatgcgctaa gtgtcaaagt cttgcaggaa ggcaaacggg taaggatcat
    19621 catcaaagat aatggaaaag ggatgactga aaaacggcta aaacagattg aagaaaaact
    19681 gtctcatcct aaagtggaac ttcacggttc tatcggtctg caaaacgtga atgaacgttt
    19741 acgtgccagc ttcggcagca gttattatat gtcgcttgag aataatgaga caggtggact
    19801 gaccgtttct attacattca aggagggatg agcgtgtatc aagtgttatt ggtagatgat
    19861 gaatacatga ttttgaatgg gttaaaaaag ataattgatt ggcaaagttt aggctttcaa
    19921 atagtagcaa cagcagagaa tgccaaagaa ggactagctg tacttgaaca gcgacagatt
    19981 gatttagtag taacggatgt gacaatgcca gaaataaatg gactggaatt tattgaagcg
    20041 gctcaaaaag aacgtcataa ttttgaattt atgattttat caggatacca agaattcgat
    20101 tatttgaaag gaggtatgca attaggcgcc gtgaattatt tgatgaaacc cgtgaataag
    20161 ttcgaattgg ttgaaagcct caagaaaatc aaaacacggt tagatcagca aaatgaacaa
    20221 aaaaatcaac aagaaatcta tcaagaactg ttattcagtc aatggttgaa tgaagaattg
    20281 gatgaaataa gtgaagaaga aataatagga caaataagtg agaagcagca taggatagta
    20341 ttgattcaac tggcaagaac tcatgatact cttatcaata gatggttgca agaacggcaa
    20401 caaatctttt actatcaacg aaattacgga gaccgtgtat tgatcacgtt gttgttggag
    20461 gcagatgaag ttgatggctt ctgttgtttt gttcaagaaa actttcctga tcaggaatgg
    20521 ctgatcagta tcggggaaga gacagattct ttggacaaaa tccctgaaac attccaacaa
    20581 gcgaaagaca atctacagtt gcatcaattt tatggagata aagacgagca tgttttttac
    20641 gcagtacaag cagaaacaaa agatcaaacc atcgattttt caagtttcag acgcgtgctg
    20701 caaaataaac aattggaagt cgctcaaaaa atgatcactg atttttttga acagtttcag
    20761 ttagcagcca tgatgccgga agatatacga tattccagtt ttttactgtt tatggagatt
    20821 caacgggaat tgattgatct ggaagatgag gaatatttac aaggaatcga aaaaatccat
    20881 caggcaaaaa cggtgcaaga acttcagcag ctacttctgt cattcatcaa aaagcatcag
    20941 agacagaaaa agtacagtga caatgtggaa aaagtaatcg taatcctgca ccagcattat
    21001 caagaaccac ttacactcaa agaagtgtca gaaaacctgc atttaaatgt catgtatcta
    21061 ggacagctgt tcaaaaaaga aacgaaaaaa agtttttcag cttatttgaa ccatttacgt
    21121 atggaaaaag cgaaacaact gcttttgcac agtaatcaaa acatcaatga gatagcaagt
    21181 gagattggct acaacaacac cacgtatttc tctaaattat tcaagaaaat cgtcggcaga
    21241 tcccctaaag agtaccgaga aaacagtgga gaatcataaa agaaaaagcc aatgtaatga
    21301 aataacttat aaaaaattgt ctagaaatct aattttgggc tgttcttatt acttgaggca
    21361 aggcagcttt ttcatgacct ctcgataaac ggagttcaaa agccagtttc tcggtattaa
    21421 gccaaattta tgcaaaaatg tggaaagcca ttttcacaaa atttttctta atactcgaaa
    21481 ctaaacagct ttttcacgac ctctccacta aacggtgttc aatcagctga ccctcggtat
    21541 tagctcaaaa aatggcaaaa tatgaggaac tattttgcca tttgtctctc taatactcag
    21601 gtcagaacgc tgatttcaca acctctcaat aaacggtgtt caaaagcttg catctgcggt
    21661 aataagacca accaaacgaa aaatatgagg atctattttt gcttggctgt tcttattact
    21721 tgatgcagga cagctttttc acaacctctt gttaattata gtttgataag gaaaaaggca
    21781 aatcttttgc ttgatatttc ttagggtgag ggatgatccc taattggtaa gagatcgtgc
    21841 cgcctttcag caaatcttga tgggtaaaat ataatttcgt atattctctg tccatccgtt
    21901 tgattttatg gataaattgt tgctgcggcc gattaggcgt tgcttcaatc gtcaattcct
    21961 ttccatttga caaaagcaaa acagccttgt caattaatgg cataccgata acatattcac
    22021 cggttccagg cgtgacagga taaaatccca gactattgaa gatataccaa gcggccatac
    22081 ttccattgtc ctcatctcca gggtaaccca ttggagaatc gtcgaaggtc tgagtcatca
    22141 actgtttaag cagtggctgt gccatttcag gttgtcctat ataactgaac aagtaaggat
    22201 agtgaaaact tggctggttt gaaatagcta attgtccaaa ttctatcgcg gccatctcac
    22261 tcatttcgtg gatctcaaaa ccatatcctc ctgggtcaaa cgttgggggc tgattgcaca
    22321 gctcaatgag tttttcttta tatctctcac tgccaccata ttttccgatc agtcctgaaa
    22381 aatcgtgata aacagcaaag ctgctttgcc aagcacttcc ttctgcataa tcttgtcccc
    22441 aacgattagc tgaaaatggt tctctgaact tgccatcttt atcttttgaa cgcataaatc
    22501 ctgactcata gtcaaagata ttttgatagt tcaatgattg ctgctgatat ttcttcgtca
    22561 tttcctcttc ttctagtacc ttggcaactt ggctaataca aaaatcgcta taagcatagt
    22621 ctaatgtgtg attgactgat tcgtggtagt gattaggaac atagccatat gtcaagtaat
    22681 cttctgttcc ttggcgtcca taatttggat gtttgctttg aaccgtagca cctttttcca
    22741 ttgccgttag aaattcaggc atcaagtctc gtcggatatc ttttacagca gcatctgcaa
    22801 tgaccgcatc gatcaatgtc ccaggcataa gtccacgttc atcaggagac agccattttg
    22861 gcaaatagcc actttctcta tagctgttca aaaaaccttc cagcatttct tcgtattttt
    22921 ctacagcaat caaagaataa agaggataaa ccgttttata cgtatcccaa aacccattat
    22981 ttgtatagag aacaccaggt tttatagatc gactagtcgt atcatagtgg attttttgcc
    23041 cactaggatc aatttcataa aatgtttgtg gaaataaaaa cacccgatat aaattatgat
    23101 aaaacgtccg caattgttgt tgatcatggt gtgtgagctg tatacgatca aaataggact
    23161 gccattgttc ttgactgtga gcaaggtaag tctctttgct ttggttttgt tctagttcca
    23221 ggttataagc tgcttgttcc gagctgataa aagatgtccc aaatcggata gtttgttttt
    23281 ttgtctgttc aaaatacaac gggatcgagt cgctattttt gttggaaaca gctaactcat
    23341 caattgttaa aggatattca aatctcaata taaaataaaa agaaaaatca ggatcttctg
    23401 aaccagcaaa atgtgtgatt tttccttcca ctgtgtaagg atctataaca aagagctgat
    23461 ggcgtcccgg aaaggataaa agtaaaccgc tgtcattttt gctgtaatca atagataaga
    23521 ttccgccata catgctaggt atcaatgtag aacagatgcc ataacgtaat tgacagatcg
    23581 acagatgagt cgggcaaaat atactttctt ctgggcggta agaactttga gcatggaaaa
    23641 gggtgttctc ctgtagtttt ccattgatcg gcgtaaacag catatgactg aaatcaccca
    23701 tccaaggact tggttgatgg gtaagacggt agccttgaaa aacgcggtca tcaggatgaa
    23761 accaccagct gccgttttga tccgtagttt gcggtgcaaa ataattcatc ccaaaaggaa
    23821 cgccagtata aggctggcag ttcccattag agaaatttgg ctgattcgct gttgcatgtc
    23881 tagtatctat ttgaccgata ttcattttat ttctcctttg ttgattgtct tttgatgaat
    23941 gaaactggaa atttactatc ggatggtcga tcttttgtct ctaaccaagt gaccaaagca
    24001 tctcccgcaa gcttacctaa ctgttcaaaa tcttgtgcga tggttgtcag tggcgggtca
    24061 acaagagctg ctgcttggat atcatcaaat ccgataattg aaaaatcttc agggatatga
    24121 taaccatgct gtctcaagag attcatggtc tggatagcga caacatcgtt ttcacaaaca
    24181 aaagcagtta cttcatactt agtgatataa gaaagcaatt catctaatgt cgcgcttttg
    24241 tcatccagtg aagtgtggaa agtcaaacca gctttgtcca atgcttcaag gtaaccaagg
    24301 taacgctgtc gaacggattg tggatgagtc tgcgtaccaa acaaataagc gatttttgaa
    24361 tgtccctgtt cgatcaaata agaggcagca agcgcaccgc cttggaaatt atcggaaaga
    24421 acggtaggaa aaggtaactc atagattttt ttatctaata tgacgacagg gaattctttc
    24481 aatgaaagct caaaaagtaa gtcaagatgt tgttctgtat caagagcata ataaatcaat
    24541 ccgtcaaatt cattgattag ctcttttgct gttttatttt gtagaaagtc aagcgtcgtc
    24601 atcatgacat caatctgttt atcttgcatc acagggttca atcccttgct aaaatcacca
    24661 acagacaaat cagcgacata tggaagaaga aacagaatcc gtcgtgtttt tgtaggagaa
    24721 gcaagaatct gttccggaga agcggattta acgaaactgc ctttcccgcg aacgcgataa
    24781 ataagtccta attgctctaa ttcagtcaat gcacgtttgg aagtgattct gcttacttta
    24841 taggtacgtg aaagttcttt ttcagttggt agttggctgt ctacaggtaa ttcaccagtt
    24901 aaaatagccg tttgcaaatc attaaatatt gtctggtaca atggttgagt catataaaca
    24961 cctcttaatg atatatcata ttcaaataat accataatgg atgaaaaagt aaataaaaag
    25021 atattttttg aaagcgtaga cataattggc tgtatgaagt ataatcttta aagatatatc
    25081 aaatagaaag aaggttttat tagtggttta taaagatata ccaaaatcgg tgcagaaatt
    25141 tatggaagat atcacacaaa aatgtgggga tatccatgcg gactgggcaa agaattttac
    25201 agcagcattt gccaatactc tgttgacaac agtcaaaaag caaaatgacg gaaccacctt
    25261 tttattgacg ggagacattc cagcgatgtg gttaagagac tctactgcac aagttcgtcc
    25321 ttatttagtg attgcaaaag aagatgagga attagctgac atgatcagcg ggttagtcaa
    25381 aaagcaattt ttttatatca atatcgatcc atatgcgaat gccttcaacg aagaagctaa
    25441 cggtgctggc caccaaacag atcatacaaa aatgaacgat tggatctggg aaagaaaata
    25501 tgaaattgat tctttatgct acccaataca gttggcctat cttctttaca aaaatacagg
    25561 aaaaactgat caattcaatc aatcttttat tgaaggtatc aaaaaaatct tggacgtgtt
    25621 caaaacagag caagatcatg aacactctcc ttatacattt gtacgagaca cgacaagaaa
    25681 agaagatacg ctgatcaatg acgggcgagg tgcaaaaaca gcttttacag gaatgacatg
    25741 gtctggtttc cgtcctagcg atgatgcttg ccgttatgga tatctggtac catccaatat
    25801 gtttgcggtg gtcgtgttag gttatgtgga agaaattttt caaacgatac tcgatgataa
    25861 agaaattgta cagcaaacac agaaattgaa gaaagagatc caagagggga ttcagaatca
    25921 cggacgaacg gttaatcaaa aagggcaaac gatttatgca tatgaagtag atggaatggg
    25981 aaacgccaca gtcatggatg acagtaatgt gcccaatctg atcgccgcac cttacttggg
    26041 atattgttct gaacaagagg aagattatct gcaaacaaga agaacattgt tgagtaaaga
    26101 aaatccatat ttctatgaag gaaagtacgc aaaaggaatt ggatcctccc atacgccaga
    26161 aaaatacgtt tggccgattg cattagctat ggaaggaatg acaacgtcag acaaatctga
    26221 aaaagagcgg atattgaatt tacttgtagc aactgacgca ggaacacacc ttatgcacga
    26281 aggattcgat gtgaacaatc ctgaaaatta tacgagagaa tggttttctt gggcaaatat
    26341 gatgttttgc gaattagtca tggattactt tgatattaga gtagaaaagt aggaggtatc
    26401 gtaaatgaaa aagaaagtat atatcatttc tcatagccac tgggatcgtg aatggtacat
    26461 ggcttacgaa cagcatcaca tgcgactgat tgaattgata gacgatctac tagagctatt
    26521 tgaagttgat ccgtccttca acagttttca tttggatggg caaacgatta ttttagatga
    26581 ctatcttcaa gtaaggccag aaaaaagaca ggcaatccaa caagcaatca atgaaggaaa
    26641 attgagaatt ggaccatttt atatcttgca agatgatttt cttatcagct cggaatcgaa
    26701 cgtgagaaat atgctgattg gcatggaaga aagccgtaaa tggggaaccc ctgtcatgtt
    26761 aggctatttt cccgatacat ttggaaatat gggacagacg cctcaattaa tgaaacaagc
    26821 tggtatttca gcagcagcgt ttggccgtgg tgtaaaacca attggctttg ataatcaagt
    26881 tttggaagca gaaaactatt cttcccaata ctcagagatg tggtggaaag gccctgatca
    26941 aacagcaatc tttggtctgc tatttgccaa ttggtatagc aacgggaatg agattccagt
    27001 agaaaaagaa gcagctcttg ccttttggaa gcaaaaatta gcggatgcag aacaatatgc
    27061 ctctacgaac cacttattga tgatgaatgg tgttgatcat caaccggtac aaaaagatat
    27121 atcaaaagca atccatctcg caaatgaact gtttccagat tacgagttta ttcattctaa
    27181 ttttactgac tatctggaag cagttcaaaa agatgtgcca gaagacttag gctcagtaga
    27241 aggagaattg acaagtcaag aaacggatgg ctggtatact ttagccaata cagcatctgc
    27301 tcgggtttac ttaaaacaat ggaataccaa ggtccagcga cagctagaaa atattacaga
    27361 accacttgcg acgatggctt atgaagttag tggaaactat ccccatgatc aattagatta
    27421 tgcttggaag acattaatgc aaaatcatcc tcacgacagt atttgcggtt gttcagtcga
    27481 ttctgtccat cgggaaatga tacctcgttt tgaaaaagca gatgaagttg gaaagtatct
    27541 tgcacaagat tccttagaac agctgaccgc agcaatcgat acgaccggat tcccaaaaga
    27601 cagctttcca tttgtcattg taaatactgc aggaatggat aaaactggtg aagcagaaat
    27661 aaccatcgaa ttagaaagaa aaagattcgc tgaaggaata cctgaacaac tatatcaaga
    27721 gttagaaaat ttgcctaaac gtaagtatca tgtagaaaca aaaagtgggg ccactatacc
    27781 agctatttta tctgaagaaa ctgtccaatt tggttatgat ttgccgaaag accgtttccg
    27841 tgtgccatat atggcaagaa tgatcaaagt gactttacca ttagaaaaca tgccagcatt
    27901 ttcttgggaa acattcgcgc ttgtagaagg ggaagcgaaa gcagaagaaa aggaaacaat
    27961 gattcatcag agtggacgaa tcatcgaaaa cggccctcta catttgacga ttgaaaaaaa
    28021 tggtaccatt acaatggaag atcgcaaaaa caaacgaaag ttgaataatc tgcatatttt
    28081 tgaagatatc ggcgatatag gaaatgagta tatctttaaa caacctttct gtgatcagcc
    28141 aatcctatcc agtaacaaag aaaactctga agtaaaagta ttagtagata caccggaaat
    28201 agcaaaaata agtattctac aggaaatgga aatccctgtt tctgcagacg agcgtttaga
    28261 aaaagaacag cagatggttg tggaatttcg ttatcgaaaa gcagaacgat ccaaagaaaa
    28321 acgaatcctt caaataaaaa caatcatgac aatcagaaaa gatagtaaaa aaatcgactt
    28381 tgaaacgact ctcgacaatc agatgaaaga tcatcgctta cgtgtcttgt ttcctacaaa
    28441 gttacatgtg gaacatcacg aagcagatag tatttttgaa gtggtgaagc gcccaaacca
    28501 tgtgtctaaa agttgggaaa accccacgaa tccgcaacat cagcaagcat ttgtcaatat
    28561 tcatgatgaa gagtacggtg taacagtcgg aaactttggt ttgaatgaat atgaagtaac
    28621 agaagacgga caaatcgcag tgacgctact tcgcagtgtg ggagaactag gcgattgggg
    28681 atattttcca acaccagaag cacagtgtct aggtgagcat cggttcaatt acagcattga
    28741 actacatgga ccagaagaaa aattctctac ttatttgcat gcatacgcag cacagattcc
    28801 tttttccact caacaaatca aacatcatga aggaacattg atttcaaaac aacaatattt
    28861 aacgataaaa agtgaaacgt ttgcaattac agctttgaaa cgcagcaagt ttagtgataa
    28921 agtagtagta agaggtttca atatgtctag tcatttagaa aaattggaaa taactaaaga
    28981 caatggaaaa actgtcatat tgaatctgct tgaagaacca acgaagcaag cggttgtccc
    29041 aataatccaa ccttatgaga ttcggacaat cggattcgag gaggaaaacg aatgtatggc
    29101 ggaatagaag caggcggaac aaaatttgtg tgtgcggttt cagatggtgg agaaatttta
    29161 gaaaaattaa gtatccctac cacaacacca gaagaaacat tggatcaggt atttgaattt
    29221 tttgatcgtt atgaattgaa gggaatcggg attggctcat tcggcccgat tggtatcgac
    29281 aaaacaaaag ataattacgg atacgttttg gcaactccca aaaaagggtg ggcacaattc
    29341 gattttcttg gcacaatcaa ggaacgctac aatattcctg ctgcttggac aacggatgtc
    29401 aatgctgctg catatgggga attgaagcaa ggagcagcac aaggaaaaaa tagttgtatt
    29461 taccttaccg tgggaacagg aatcggtgca ggtgtcgtga tcaatgaaga aattttttca
    29521 gggatcgcac atcctgaaat gggacatatt tgggtgaaac gccatccaga tgaccattat
    29581 gaaggtactt gtccttatca taaagattgt ttagaaggat tagcagcagg accgtctatt
    29641 gaggcacgca cagggatcaa agggcaagat ctgccgcaag atcatcctgt ttgggctatt
    29701 caggcttatt atattgctca agcactgatc aactatacgt tgacgttagc acctgaaaag
    29761 attattttag gcggtggggt aatgaatcag gatcatctcc ttcaaaaaat ccgtcagcaa
    29821 tttgttgaat tgatgggtgg ttatatggaa acacctcaag taagtgaata tatcgttcgt
    29881 tggggactgc cgaatgaaag tgggattatc ggtagtttgc tgttagcaga acacgaacat
    29941 caaaagtaag cagacaaatt acacaaaaag aacagagtct tatcattatc tgctgataag
    30001 actctgttct tttttatttc tattcccata atccaggtaa agctttaccg actatttttt
    30061 cttgagtaaa tggatggatc atgtgcagtt ctaatgcatg gagcatcagt cgttcagctg
    30121 ttctattttg gtagagaggg tctccaacga tcggatgtcc cttatgagct aaatgaactc
    30181 tgatctggtg tgtacgtccg gtttccaaaa cacaatagac ttgtgtttgt tcagaaagta
    30241 ctttgttgac agtgacatgt gtcaaggcgc tctgaccatt tttatcatct acgacccttt
    30301 ttctccgatc atggcgatct cgtccaattt tattggtgat ggtcatatct ttggcaattt
    30361 ctccccatac gatggcttga tatctccgat agatcatttt ttgttccaac attcttccaa
    30421 gtatcggcaa gacaaatgga ttttttgcaa ataaaacggc accgcttgtt tctttgtcca
    30481 agcggtggac aacgtaaggt cgttgctgtt tagtgtcgag ataagcagct aaatgattga
    30541 gtaaggtact gttttctgtc ggttcatttg gatgagtctt cattcctgca ggcttatcca
    30601 ctataatcaa atgctcatct tcatataaaa cacggatgtg cttcgcatct cctaattgaa
    30661 cttcttgata attataatct gtttcttcga ataacaatgt gatctggtca ttgcccttta
    30721 cttcatgatg gaacaaagca ggttgttgat tgatccaaac gtttttacgt atacgcaaaa
    30781 agtgtcgaac ctttctagga actaaccatt ccttttcaag caagtcgcga attgttgttt
    30841 caggatggtt cataggtaaa gtgatcgtga ttttcacaag tgtactcctt tcagtgcaat
    30901 tgcagactaa ttgtaccatt tttaaaacat atttaagaaa aaacttacat ttttctgttc
    30961 attcttatgt tactctactt gcactaacgt aaaaaaacat attgaactac aacttaagca
    31021 ggaagaaaac actctttcac tatggtaaaa taagacgatt gaatcaaata gacaggagaa
    31081 gaagcaaatg gattttcaaa gcattttaga aaagataaaa gctgctttga tcagtttttg
    31141 gcgttggatc agaccatatc tcggacaatt ccatcgatgg cgaaagcgca tctggaaaaa
    31201 atatcatgtg aacaaattga ttttgctgat tggtttgatc tgtgtcttag tgacaagtat
    31261 ctacctcttt attttagcaa aacaagcaaa tgtcgaaacg ttaaaatcag gattaagcca
    31321 atcgacagtc atttatgata agaaaaacga agaagctggc acactgtacg cccaaaaagg
    31381 aacatatgta gaattaaacg cgatttctcc ctatatccaa gatgcagtaa tctcgacaga
    31441 agataggaat ttttacgagc atcacgggtt tgatatcaaa gggattgccc gtgcagcagt
    31501 ccgaatggtc ttgtctggcg gaacatcagg gggcggagga agtacgatca ctcagcagct
    31561 tgccaaaaat gcgtatctga cattagacca aacgtttgat cgaaaagcga aggaattatt
    31621 tttggcaatc gagatcgaaa agaagtacag caaagaagaa atcttgacca tgtatcttaa
    31681 caatgcctat tttggcaacg gggtatgggg tgtccaagac gcttccagaa agtattttgg
    31741 agtagatgca acgaatgtta cgctgtctga ggctgctact ttggcaggaa tgctgaaagg
    31801 tcctggtatt tataatccga tcgatcaccc aaaaaatgcg aatgatcgga aaaacacagt
    31861 actaagtgta atggaagaaa acggaaaaat cacaaaagaa caagaacaag cagaaagcca
    31921 gacagatatc agcgcgtatc ttaatgacac atacgataat tcagattctg gttatcgtta
    31981 tccttattat tttgatgcag tgatcgatga agcagttaac gagtatggtc ttgatgaaga
    32041 agatgtcatg aataaaggct ataaaatcta tacagcttta gatcaggatt atcaaaagca
    32101 attagaagct acttatcaaa acagtgcatt attcccagca aatgcttcag atggtgccat
    32161 ggtccaatct gcttcagtag ctttagatcc gaatacaggt ggtgtacaag cactagtagg
    32221 acgaagagga gatcatgtct ttagaggatt cagctttgct acacagatgc gtcgttcacc
    32281 aggatcaacg atcaaaccat taagcgtata tacaccagca ctggaagctg gatacaagcc
    32341 ttccagtatc ttgaaagacg aaccgcaaag ttattacgat gcgcataatt ttgacgggac
    32401 ataccaagga gaagtaccga tgtatcaagc tgttgcacaa agtttgaatt tgcctgctgt
    32461 atggcttttg catgagatcg gtttacaaaa aggatatgat aaagcagaag aattcggcct
    32521 gcctttagca aaatctgata agtattatgg cttagccctt ggtggactgg aaaaaggagt
    32581 atctccattg atcatggcgg gtgcttacag tgcatttgcc aatgatggtc agatgtatac
    32641 gccgcatctt atcacgaaaa tcgttgattc gactggagcg gtgatcgtcg ataacacaaa
    32701 caaaaaacca aaacaggtca tatcaaagga aacagccgat gaaatgacca gcatgcttct
    32761 tggtacattt tcaaatggaa cagcggctgc tgcgaatcct gcaggttata cgatagcagg
    32821 gaaaacagga accacagaaa ctaatttcga tgccacaaaa gcaaatgacc aatggatgat
    32881 cggttacacg ccagacgtag tgatttcgac atggatgggt tacgaagaat ccagcaagct
    32941 gcactatttg gaagggacaa gtggaaatgt tgtcgggaaa gtcttcaaat cagcggcaga
    33001 aggcatcctt ccgtatactt caaaaacagg atttagtgta gcagatgcct atgccacagg
    33061 aggacaagtg gtagctgcag atcaagttgg aaacacagga aattctaatg gagagtctgg
    33121 aaattggcaa gataacttga atcaatatgg tgaacaagca aaagaagggc tgaaaaactt
    33181 cggcgatatg gtgaaagaag gcgttcaggg aattggtgaa gcggctaaag acctctggcg
    33241 gaaatatcaa ggagagtagc ccctttgaaa acgaataacc ccatgttaca atagagagta
    33301 actactaaaa gaaaagaggg cataacatgt caaacatcta cgatagcgcg aatcaaatcg
    33361 aacgtgagat ccgtgaatta ccagaattca aagcgttgaa agaagcgtac gagaaagtac
    33421 aagcaaacga agaagcacac aaactgttca aagacttcca agcgatgcaa atcgaattac
    33481 aagaaaaaca aatgagtgga caagagttca gcgacgaaga tgcaatgaaa gcacaagaaa
    33541 tggctgtgaa aatccaatca gaagaagtaa tcaatgactt gatgcaaaaa gagcaaggct
    33601 tcagtacaat catcaatgac ttgaaccgta tcatcatgac accagttcgc gacctttaca
    33661 gcgaataaaa ctattttatg aaacaacaga gggtgtgaaa gagacgttcg gccttgagcg
    33721 atgacaaaat tgcttcttat tgccgatagg ctgtctcttg aacaccattt accaagaggt
    33781 cgtgagccta ccgtttaact ctgagttaca aggaacaatt tcaaaaaaca gcttctcatg
    33841 tttgatgact attgtgactt gtgaccgagg agttggcagg agaacaccgt ttattcagaa
    33901 ataggagcta cgacaaaagt cttgtcatag ctcctatttt ttgtcattca tttttcaagc
    33961 ggtagaaata gcctttttga gctaacagtt tttcgtaagt acctatttca acgatttttc
    34021 cttcatcgat cacaacgatt ttatctgcct ctttgatcgt tgaaagccga tgagccacaa
    34081 taaaagtagt gggtgtttct gtcaggtaat tcagggcttg ctggattttt ttctctgact
    34141 ggttatctaa agctgaggtt gcttcatcta gcaaaatgat ttttggctga cgtatcaacg
    34201 ctcgtgcaat ggagatacat tgcttttggc caccagacag cttgttgcca tgttcgccta
    34261 ctaaggtatc cagaccatca ggaagctggt caatcagtga agaaagttgc gcagcttcga
    34321 tgacttcatc taaacgttct tttgatacgt ttttcaagcc ataagtgatg ttctctttga
    34381 tcgaagcgga gaacaacaaa gtggtttgag gaacaacggc taaataattt cgcacacttc
    34441 gcatatccag ttcatccaaa ggctgatcat ctagaaaaat ttttcctgaa gtcggctgga
    34501 taaatccaat caataagtta aggagtgtag attttccaga gccagatggc ccaacaaaag
    34561 caattgtttc tccagctttc acctctagag aaaaatgatc gataatcggt ttgagtgagt
    34621 ccggataaag aaaagaaaca tcctcaaaac gaaaatttcc atccaaatgt tggatttttt
    34681 tcttaccgtg ataagtttct acatcgccag caatcaacag ttcgccaata gaagtgatag
    34741 attccatgcc ttttgaaatc gtaggaatca gcgtgatcat tccagaaacg gaattgacga
    34801 tcgtcgtgaa atacgtctga tacatcacga catcgccggg ttggatgcgc ccttgtaaag
    34861 ctaatatccc agtgaaaatc aaacaaatca gctgaaatat ctgaaaacta gcccagctga
    34921 ctgatccgaa aacagattgc aggatatcta agtggaatcc tttttcatag atttgagtaa
    34981 aatgcttatt taagcgagtt gtttcgtgtt tttctaaccc atgagctcga gcaatcggtg
    35041 ctaattcgat cacttcgatc acttttgcag aagtctcttc catttcttgc ctgaagtctg
    35101 agttggactg tctgatcctt ttacggaaaa agagagtaac gaatgcggcg agaggaacag
    35161 tcaataagaa aaacatgaaa acaacaggac ttttataaag cgtgatacct aaagctacga
    35221 ataaattgac agcgatattt aatccgctga caaatacttg agaggacaac gtttggatag
    35281 cttctacatc acggatgatc tttgactgga ttcgtccaga ttgcaattct ttttgataag
    35341 gaatagatag ttcttggatt ttcttgataa gggtcgctct tagtccagct tctacttgac
    35401 ggatcgaagt gctatggtat tttacatgaa aataattcgt caatatattt tgaccaatca
    35461 gacaagccat caaaaaagcg ttgaaataaa tggcctgcaa ctgagaagaa tctttcgatg
    35521 aagctgcatt gattacattg gcaatagcga tcggcattaa ccaagatgga agatgtttga
    35581 taataaaaaa gagtcctgac atggcaaaac gccaataatt tccctgataa agatgcagca
    35641 atgtctgaaa agcatttgca tgatattttt gataactttt ctgtattcgt tcctccataa
    35701 tcttctcctt aacaatcttg gtaaataaac tgcaagtgtt ctattgcttc aggaacataa
    35761 atctcaggag tagcttcaag agtggcgtac attagaggac gctcatattt gatataatgc
    35821 agtaccgggc ggaaatctaa acagcccttg ccaacaggta ccatgcggat ctttccattt
    35881 tccatgatat agtctttgag atgaagcgct gtaatataac catgtaaatt gtccaaggca
    35941 cctcggatca ctttttcctg atcagcatga ttttctttat gcatcaaatt cgcacaatct
    36001 aaaatgattt ttaaatttgg tgaagctact tcatcgatta aacgtttagc taactgatag
    36061 ttatagattg gatgattgat ccctgcttca acagcaacag ttacgccaaa attttctgca
    36121 tgagcgacta attcaatgat agaatttctg gcgatctgat aagcttcttc agtaaaattt
    36181 tcttttgtgt aaccgacaga gacactaccg gtttccgtag caatcatctt ggcttgatag
    36241 gcagagcaca gagcaatttg ctgtttgaaa gttgccaatg cttgtgcacg aacttcttga
    36301 tttttgctgg ataggttgat atagcatcct aaaatggaaa gttcgatatt gtttttttgg
    36361 aagtattcac caaaataggc agcactccca agtgtcatgt cagttaattt aagttgttca
    36421 ggaaatgctt tttgtggtgc aaattggata tgggacaagt gaagctgtga tactttttga
    36481 acgatttctt cacgatttcg aacatctaaa tcgtgggcac ggatgcctaa atttaatgga
    36541 aacatctcaa actccttttt gtaaataatt agcaagaggc agacgggatt cttttatcaa
    36601 ttcaacaatt attcgggcaa ctttatcagc gccttctgga ctaaagtggg tattgtcttt
    36661 tagtccttct gggtaatttt tacaagtatc aggcataaga tgcaggtaga atttttttgt
    36721 ctcttctttt gagaaggtct ggaacaattc ttgtgatctt gagaacacat ctaaaagagg
    36781 aatatggtgt ttctcagcaa atgcacgcat cgcttttgga tagtctccaa gtatatctgg
    36841 attcaatgtt ccgttttcta agtaatctcg tcgcgtcaca gatgtcagta agaccatttg
    36901 gacgttcgct ttttttgcgc attcaaaaaa tatctgcaga ttttttaaat agctaccgaa
    36961 gggaagcgta cctctttctt cttggatttt ctggtcgtta tgtccaaatt gtacaaataa
    37021 gaaatcattc ggctgaaact gtgaagctag acgggctaac cttccttcct ggataaatga
    37081 tttggtgctg cgtccattct ttgcttggtt attgatctgg atctcgggtg aaaaataagc
    37141 agagattttt tctccccaac ctgtctcggg acgcttgttt tcatcttttt ctgccgcagt
    37201 agaatcaccg gcaatatgga tagtaggcat gttatctctt ctttcataaa ggtttatagg
    37261 caatgaagca gaacagcaag agaagaagac ggagcaa
//
LOCUS       DAZUKZ010000019        34397 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000019 DAZUKZ010000000
VERSION     DAZUKZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.019"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACG8EF_000879"
     CDS             352..1041
                     /locus_tag="ACG8EF_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4946225.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACG8EF_000880"
     CDS             1028..2236
                     /locus_tag="ACG8EF_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS4946226.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACG8EF_000881"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACG8EF_000881"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFS4946227.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG8EF_000882"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG8EF_000882"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFS4946228.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ACG8EF_000883"
     CDS             complement(6339..7034)
                     /locus_tag="ACG8EF_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFS4946229.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG8EF_000884"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG8EF_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFS4946230.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ACG8EF_000885"
     CDS             complement(8233..8424)
                     /locus_tag="ACG8EF_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFS4946231.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ACG8EF_000886"
     CDS             complement(8445..9335)
                     /locus_tag="ACG8EF_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFS4946232.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ACG8EF_000887"
     CDS             complement(9322..10089)
                     /locus_tag="ACG8EF_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS4946233.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG8EF_000888"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG8EF_000888"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFS4946234.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG8EF_000889"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG8EF_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFS4946235.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG8EF_000890"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG8EF_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFS4946236.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ACG8EF_000891"
     CDS             14660..15043
                     /locus_tag="ACG8EF_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946237.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG8EF_000892"
     CDS             complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG8EF_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFS4946238.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ACG8EF_000893"
     CDS             complement(15987..16805)
                     /locus_tag="ACG8EF_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFS4946239.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG8EF_000894"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG8EF_000894"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS4946240.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG8EF_000895"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG8EF_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFS4946241.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG8EF_000896"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG8EF_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFS4946242.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG8EF_000897"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG8EF_000897"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFS4946243.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20824..21066
                     /gene="yaaA"
                     /locus_tag="ACG8EF_000898"
     CDS             20824..21066
                     /gene="yaaA"
                     /locus_tag="ACG8EF_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFS4946244.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21053..22177
                     /gene="recF"
                     /locus_tag="ACG8EF_000899"
     CDS             21053..22177
                     /gene="recF"
                     /locus_tag="ACG8EF_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFS4946245.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22174..24120
                     /gene="gyrB"
                     /locus_tag="ACG8EF_000900"
     CDS             22174..24120
                     /gene="gyrB"
                     /locus_tag="ACG8EF_000900"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFS4946246.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24140..26611
                     /gene="gyrA"
                     /locus_tag="ACG8EF_000901"
     CDS             24140..26611
                     /gene="gyrA"
                     /locus_tag="ACG8EF_000901"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFS4946247.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26847..27146
                     /gene="rpsF"
                     /locus_tag="ACG8EF_000902"
     CDS             26847..27146
                     /gene="rpsF"
                     /locus_tag="ACG8EF_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFS4946248.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27196..27708
                     /gene="ssb"
                     /locus_tag="ACG8EF_000903"
     CDS             27196..27708
                     /gene="ssb"
                     /locus_tag="ACG8EF_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4946249.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27734..27970
                     /gene="rpsR"
                     /locus_tag="ACG8EF_000904"
     CDS             27734..27970
                     /gene="rpsR"
                     /locus_tag="ACG8EF_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFS4946250.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28113..30086
                     /locus_tag="ACG8EF_000905"
     CDS             28113..30086
                     /locus_tag="ACG8EF_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFS4946251.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30092..30544
                     /gene="rplI"
                     /locus_tag="ACG8EF_000906"
     CDS             30092..30544
                     /gene="rplI"
                     /locus_tag="ACG8EF_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFS4946252.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30818..32185
                     /gene="dnaB"
                     /locus_tag="ACG8EF_000907"
     CDS             30818..32185
                     /gene="dnaB"
                     /locus_tag="ACG8EF_000907"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS4946253.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32965..34257
                     /locus_tag="ACG8EF_000908"
     CDS             32965..34257
                     /locus_tag="ACG8EF_000908"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFS4946254.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10495 a         6692 c         6807 g        10403 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    20761 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    20821 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    20881 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    20941 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21001 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21061 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21121 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21181 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21241 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21301 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21361 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21421 agtaaaaggc agcccgcaga ccaggcggaa gtttttagat atggaattag gccagatcaa
    21481 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21541 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21601 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21661 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21721 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    21781 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    21841 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    21901 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    21961 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22021 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22081 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22141 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22201 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22261 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22321 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22381 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22441 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22501 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22561 gttgttaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22621 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22681 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22741 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    22801 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    22861 ggcgggatta aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    22921 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    22981 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggcgga
    23041 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23101 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23161 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23221 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23281 ttcttgctgg aaaacccaag tgtgggccgt caaattgttg aaaaaggatt actagcttcc
    23341 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23401 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23461 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23521 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23581 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23641 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23701 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    23761 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    23821 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    23881 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    23941 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24001 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24061 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24121 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24181 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24241 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24301 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24361 catgggtaaa taccatcctc atggagacta tgcgatctat gagtcaatgg tacggatggc
    24421 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24481 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24541 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24601 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24661 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24721 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    24781 tggaccagat tttccaacag gtggtttggt tatgggaaaa tcaggaatcc gaagagcgta
    24841 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    24901 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    24961 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25021 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25081 tgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25141 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaattc ttgaaaacta
    25201 tatcgaacat cagcgcgaag tcatcacaag acggacagtc tttgaaaaag aaaaagcaga
    25261 agcaagggca cacattttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25321 tgcgattatc cgcgggtcta aatcagatga cgaagcaaaa gcgacgatga tcgaacggtt
    25381 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25441 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25501 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25561 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25621 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25681 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25741 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    25801 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    25861 tccagaatac gggcgcacag ccaaaggaat accaattatc aatttactag gtatcgattc
    25921 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    25981 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26041 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26101 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26161 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggttagag gaatacgcct
    26221 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaagatcaag aagtattagt
    26281 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26341 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26401 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26461 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26521 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26581 aacagttgaa gaacaaggat cagaagaata gccatcaaga ctaattgtgt aagctagcaa
    26641 gaatgaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26701 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatca
    26761 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    26821 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    26881 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    26941 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27001 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27061 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27121 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27181 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27241 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27301 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27361 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27421 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27481 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27541 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27601 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27661 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27721 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    27781 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    27841 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    27901 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    27961 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28021 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28081 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28141 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28201 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28261 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28321 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28381 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28441 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28501 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28561 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28621 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattggatg
    28681 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28741 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    28801 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    28861 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    28921 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    28981 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29041 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29101 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29161 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29221 tcgttatcaa cgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29281 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29341 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29401 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29461 caagtaaacc tctgctgact tatatcgaat cttcagcttc ttcagcttca gagctagttt
    29521 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29581 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29641 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29701 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    29761 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    29821 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    29881 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    29941 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30001 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30061 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30121 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30181 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30241 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30301 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30361 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30421 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30481 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30541 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30601 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30661 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30721 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    30781 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    30841 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    30901 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    30961 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31021 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31081 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31141 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31201 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31261 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31321 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31381 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31441 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31501 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31561 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31621 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31681 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31741 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    31801 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    31861 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    31921 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    31981 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32041 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32101 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32161 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32221 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32281 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32341 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32401 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32461 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32521 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32581 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32641 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32701 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    32761 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    32821 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    32881 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    32941 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33001 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33061 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33121 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33181 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33241 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33301 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33361 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33421 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33481 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33541 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33601 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33661 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33721 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    33781 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    33841 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    33901 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    33961 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34021 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34081 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34141 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34201 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34261 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34321 gataataata acttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca
    34381 cagtcccttt ttccgaa
//
LOCUS       DAZUKZ010000020        34330 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000020 DAZUKZ010000000
VERSION     DAZUKZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.020"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            202..1077
                     /locus_tag="ACG8EF_000909"
     CDS             202..1077
                     /locus_tag="ACG8EF_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFS4946255.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="ACG8EF_000910"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="ACG8EF_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="ACG8EF_000911"
     CDS             complement(1642..2973)
                     /locus_tag="ACG8EF_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4946256.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="ACG8EF_000912"
     CDS             complement(3122..3835)
                     /locus_tag="ACG8EF_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4946257.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="ACG8EF_000913"
     CDS             4010..5434
                     /locus_tag="ACG8EF_000913"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4946258.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="ACG8EF_000914"
     CDS             5733..6440
                     /locus_tag="ACG8EF_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS4946259.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="ACG8EF_000915"
     CDS             6427..6750
                     /locus_tag="ACG8EF_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS4946260.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="ACG8EF_000916"
     CDS             complement(6839..7876)
                     /locus_tag="ACG8EF_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4946261.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="ACG8EF_000917"
     CDS             complement(8197..9180)
                     /locus_tag="ACG8EF_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFS4946262.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="ACG8EF_000918"
     CDS             complement(9595..10365)
                     /locus_tag="ACG8EF_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4946263.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="ACG8EF_000919"
     CDS             complement(10358..11293)
                     /locus_tag="ACG8EF_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4946264.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="ACG8EF_000920"
     CDS             complement(11609..13258)
                     /locus_tag="ACG8EF_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS4946265.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="ACG8EF_000921"
     CDS             complement(13375..15978)
                     /locus_tag="ACG8EF_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS4946266.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="ACG8EF_000922"
     CDS             complement(15990..17039)
                     /locus_tag="ACG8EF_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS4946267.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="ACG8EF_000923"
     CDS             complement(17065..17379)
                     /locus_tag="ACG8EF_000923"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFS4946268.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="ACG8EF_000924"
     CDS             complement(17395..17841)
                     /locus_tag="ACG8EF_000924"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS4946269.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19764)
                     /locus_tag="ACG8EF_000925"
     CDS             complement(17842..19764)
                     /locus_tag="ACG8EF_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4946270.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(19976..20872)
                     /locus_tag="ACG8EF_000926"
     CDS             complement(19976..20872)
                     /locus_tag="ACG8EF_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4946271.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20865..21545)
                     /locus_tag="ACG8EF_000927"
     CDS             complement(20865..21545)
                     /locus_tag="ACG8EF_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4946272.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21546..22328)
                     /locus_tag="ACG8EF_000928"
     CDS             complement(21546..22328)
                     /locus_tag="ACG8EF_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4946273.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22321..23238)
                     /locus_tag="ACG8EF_000929"
     CDS             complement(22321..23238)
                     /locus_tag="ACG8EF_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4946274.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23402..23656)
                     /locus_tag="ACG8EF_000930"
     CDS             complement(23402..23656)
                     /locus_tag="ACG8EF_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946275.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            24513..27740
                     /gene="fss3"
                     /locus_tag="ACG8EF_000931"
     CDS             24513..27740
                     /gene="fss3"
                     /locus_tag="ACG8EF_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFS4946276.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27853..28167
                     /locus_tag="ACG8EF_000932"
     CDS             27853..28167
                     /locus_tag="ACG8EF_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4946277.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            28180..28554
                     /locus_tag="ACG8EF_000933"
     CDS             28180..28554
                     /locus_tag="ACG8EF_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4946278.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            28564..28899
                     /locus_tag="ACG8EF_000934"
     CDS             28564..28899
                     /locus_tag="ACG8EF_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFS4946279.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            28982..30322
                     /locus_tag="ACG8EF_000935"
     CDS             28982..30322
                     /locus_tag="ACG8EF_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS4946280.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            30400..31074
                     /locus_tag="ACG8EF_000936"
     CDS             30400..31074
                     /locus_tag="ACG8EF_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS4946281.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            31067..31231
                     /locus_tag="ACG8EF_000937"
     CDS             31067..31231
                     /locus_tag="ACG8EF_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946282.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            31307..32449
                     /gene="dcm"
                     /locus_tag="ACG8EF_000938"
                     /pseudo
     CDS             31307..32449
                     /gene="dcm"
                     /locus_tag="ACG8EF_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            32439..32729
                     /locus_tag="ACG8EF_000939"
     CDS             32439..32729
                     /locus_tag="ACG8EF_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946283.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            32988..34172
                     /gene="mobT"
                     /locus_tag="ACG8EF_000940"
     CDS             32988..34172
                     /gene="mobT"
                     /locus_tag="ACG8EF_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS4946284.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            34169..34306
                     /locus_tag="ACG8EF_000941"
     CDS             34169..34306
                     /locus_tag="ACG8EF_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS4946285.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT        10701 a         6609 c         6254 g        10766 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
    19861 ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta
    19921 ttaaatatat ataagataat acatttttct actagttctc actaatcttg tataattaaa
    19981 ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc
    20041 ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat
    20101 ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg
    20161 ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg
    20221 tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc
    20281 gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta
    20341 cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt
    20401 tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat
    20461 cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat
    20521 acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag
    20581 cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt
    20641 ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat
    20701 ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt
    20761 gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga
    20821 aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac
    20881 cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga
    20941 ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt
    21001 tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga
    21061 tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct
    21121 ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa
    21181 gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc
    21241 gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt
    21301 gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca
    21361 cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac
    21421 aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc
    21481 tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg
    21541 accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg
    21601 attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca
    21661 aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg
    21721 atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc
    21781 aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct
    21841 aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt
    21901 ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc
    21961 aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc
    22021 attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca
    22081 atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc
    22141 attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg
    22201 tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt
    22261 cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc agttaataga
    22321 ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac
    22381 taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac
    22441 ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata
    22501 accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa
    22561 ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg
    22621 caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa
    22681 gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc
    22741 tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag
    22801 tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt
    22861 ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc
    22921 gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg
    22981 ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa
    23041 ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt
    23101 acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac
    23161 tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt
    23221 ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg
    23281 ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca
    23341 aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt
    23401 atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga
    23461 aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa
    23521 ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct
    23581 tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
    23641 ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
    23701 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
    23761 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
    23821 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    23881 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
    23941 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
    24001 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
    24061 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
    24121 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
    24181 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
    24241 tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt
    24301 ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc
    24361 agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt
    24421 cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
    24481 tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
    24541 gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga
    24601 ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
    24661 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
    24721 cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc
    24781 cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata
    24841 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
    24901 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
    24961 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg
    25021 attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga
    25081 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg
    25141 ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt
    25201 caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
    25261 ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca
    25321 caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat
    25381 cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa
    25441 aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa
    25501 aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga
    25561 ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag
    25621 ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt
    25681 attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc
    25741 aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt
    25801 tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt
    25861 tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta
    25921 acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag
    25981 atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta
    26041 ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg
    26101 gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg
    26161 ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata
    26221 gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
    26281 ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct
    26341 ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta
    26401 aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag
    26461 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
    26521 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
    26581 tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca
    26641 ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga
    26701 cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag
    26761 aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg
    26821 attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt
    26881 atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa
    26941 aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag
    27001 gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct
    27061 tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga
    27121 aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt
    27181 ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg
    27241 tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt
    27301 tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg
    27361 gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag
    27421 tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata
    27481 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa
    27541 caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc
    27601 caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa
    27661 attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt
    27721 tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg
    27781 cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa
    27841 gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag
    27901 ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg
    27961 attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta
    28021 gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat
    28081 ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg
    28141 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
    28201 gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta
    28261 cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta
    28321 aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc
    28381 gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat
    28441 tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg
    28501 aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag
    28561 attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag
    28621 ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata
    28681 catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg
    28741 ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag
    28801 gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta
    28861 aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact
    28921 ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa
    28981 aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct
    29041 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag
    29101 acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga
    29161 tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca
    29221 cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct
    29281 agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa
    29341 tagtagccga accagagaga ctatcagtta cttccccaaa atctgttatc gaatgaaaga
    29401 tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa
    29461 attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga
    29521 ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga
    29581 agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc
    29641 tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac
    29701 cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc
    29761 agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc
    29821 tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat
    29881 gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat
    29941 ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt
    30001 gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc
    30061 ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg
    30121 cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa
    30181 aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat
    30241 tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa
    30301 attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta
    30361 taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat
    30421 ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc
    30481 cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag
    30541 gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt
    30601 cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct
    30661 tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat
    30721 gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt
    30781 cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa
    30841 cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa
    30901 tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata
    30961 ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta
    31021 tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga
    31081 acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc
    31141 tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca
    31201 acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaataatgt atgagccaag
    31261 agcaaaatgt tcttggcttt tttctttgca atgaatgagg taatgtatga gatttttaga
    31321 tttattcgcc ggtattggtg gctttcgttt aggtatggaa caggcgagcc atcattgtat
    31381 cggtttttgt gaaattgata aatttgctag acgtagttat aaagcaattc atgatacaag
    31441 taaggaggtg gaaatgcatg acatcacaag tgtatcagat gagtttattc aatccctcgg
    31501 accagtggac atcctttgcg gcggatttcc gtgccaagct ttttcaattg cgggaaagcg
    31561 gcaaagattt tccgatactc gaggtacttt attctttgaa atcgctcggt tcaccactct
    31621 tctccgacct aagcttttat tccttgagaa cgtccgagga ttgctcaatc acgaaggagg
    31681 ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgacg tggagtggta
    31741 ggtgcttaac tcaaaggcct acattcccca aagccgggag cggatattcc ttatcggaca
    31801 ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggaccgtctt caacatctga
    31861 tcaaaacatc agaaatttgg taaatatcaa tccttccaat cgaggaatgg gtggacaagt
    31921 ctatggttca gatggcgtag cgccaacctt aacaggtgat gaaggaatca aaatcgcact
    31981 gccagcaagg gatggaatct ctgtagcagg actattaccg ggaaagtttg aacaaggtaa
    32041 tcgagtttat gaagttactg gaaccgcccc caccttgtca acgaaacaag gtggtacaaa
    32101 aatcatgatt cgaaaaaatg aaacagaata tcaagaggtg aagccagaag acagtgtgaa
    32161 tctggctttt cctaattcca caagtcgccg aggtcgtctg ggaaaacaga gtgttcatac
    32221 cttactaaca ggagaccaac aagcgatcgt cacggatcag tatcaaattc ggaagctaac
    32281 acctagagag tgttggcgac ttcagggctt tcccgattgg gcttttgatc gagattctca
    32341 agtcaattca gatagtcagt tgtataagca agccggaaac tctgtaacag ttccggtcat
    32401 ttttgatatt gccagaagat taaaagagtt ggatgtacat ggtttatgat gagttgattg
    32461 gagaaattta ttgggtgatt ggaaagattc agtcagaccc agaattagaa gaacaattgc
    32521 gacggttaaa atttgatatt cgcaagaatg gtgtcaaagt cgctggcgat ccatatttaa
    32581 tgaatgagga gacggatgct cgaattgaaa taaatcaagt aattgcagaa tttgagcgga
    32641 ttgcggacca agcaaaagaa ccagatattc gtcaatactt gttagaaatg aaagcagagt
    32701 tggaagtaaa tggtattaca gatgagtaac ctaaaccaac acaactgact gttaggcgct
    32761 gaccgcggag gattcgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc
    32821 gttagccgac catgatgtct cacccccgta tctaacaggg gggtacaaaa acacaaaaaa
    32881 taagtaagca accattaaga atgcttgttg tatcaaagat agtaagtaat ttgtataggt
    32941 gttaactcat ttgaaaaagt taacaccttg aatttggagg tgagaacttg gcacaacgga
    33001 atttagatta tcggctatta aaagaccgac gcaacgaata cggcgtttca caaaataaat
    33061 tggctacggc ttgtggactt agtcggccgt acttaaatca gatagaaaat ggtggcgtaa
    33121 ccgcatctat aaaaaccatg aggaaaattt ttgaccaact ggaaagcttt aatcccgatt
    33181 tgcctttgac gttactgttt gactatgtaa ggattcgctt tcccacaacg gatgcacgaa
    33241 aaattattca agaaattctc catttgaaat ttgagtatat gctccatgaa gattatgcct
    33301 tttactccta tcaagaacag tatgtcatgg gtgatattgt ggtgatgttg tctcatgaag
    33361 aagataaagg tgtgctttta gaattaaagg ggcgaggttg ccgacaattt gaaacttttt
    33421 tactcgctca aaagcgtagc tggtacgact ttttcgagga ttgtctaaag gccggtggcg
    33481 tgatgaaacg cttggacttg gcaatcaatg atcgagtggg gctattggat atacctgagc
    33541 taacgaaaaa atgtcagaag gaagaatgta tctccttgtt tcgtaccttt aaaagttatc
    33601 gttccgggga acttttaaag gctgatgaaa aggacggcat gggcaatacc ttatatatcg
    33661 gcagtctcaa gagtgaggtt tatttctgtt tgtatgaaaa agactatgaa caatatatca
    33721 agttagggat tccattagac caaactgaga cgaaaaatcg ttttgagatt cgactgaaaa
    33781 atgaccgtgc ttttcatgcg attcaagatt tgttaaaagg tcgtagcatt gagagcacca
    33841 cgttttccat tatcaatcgt tacttgcgat ttgcggataa agtagaaggt aaaaggcgaa
    33901 ccaactggcc cttgaatgaa caatgggggc gttttattgg acggaatcgg aaagagattc
    33961 aattaacttc cgagccaaaa ccgtatacca ttgaacgaac cttgaattgg ctaggccgac
    34021 aagtcgcccc tacgtggaaa atggctaagg agttggatcg attgaatcgg acaacctata
    34081 tccaggatat ggtacagaat gcacgcttat cagaccgaca taaaaagatt ttggagcaac
    34141 aaagtatggc aattgaaaat ttgattatat gaaaagagga atcgtcatgg gaattgtaaa
    34201 agatattttt ttggtatttg gtggcagtat gcttggtgtt actgtgatgt gcttgatgaa
    34261 tgcaagtgca gcagcggatc gagcaatgga aaagaaagga aagtgacaag atgaatttcg
    34321 gacaaaattt
//
LOCUS       DAZUKZ010000021        33532 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000021 DAZUKZ010000000
VERSION     DAZUKZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.021"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..764
                     /locus_tag="ACG8EF_000942"
     CDS             78..764
                     /locus_tag="ACG8EF_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFS4946286.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            766..1710
                     /locus_tag="ACG8EF_000943"
     CDS             766..1710
                     /locus_tag="ACG8EF_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFS4946287.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1909..2553
                     /gene="pcp"
                     /locus_tag="ACG8EF_000944"
     CDS             1909..2553
                     /gene="pcp"
                     /locus_tag="ACG8EF_000944"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFS4946288.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2632..3909
                     /locus_tag="ACG8EF_000945"
     CDS             2632..3909
                     /locus_tag="ACG8EF_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946289.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(4093..4842)
                     /gene="pnuC"
                     /locus_tag="ACG8EF_000946"
     CDS             complement(4093..4842)
                     /gene="pnuC"
                     /locus_tag="ACG8EF_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFS4946290.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            5172..5552
                     /locus_tag="ACG8EF_000947"
     CDS             5172..5552
                     /locus_tag="ACG8EF_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4946291.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            5648..6157
                     /locus_tag="ACG8EF_000948"
     CDS             5648..6157
                     /locus_tag="ACG8EF_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946292.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            6234..6413
                     /locus_tag="ACG8EF_000949"
     CDS             6234..6413
                     /locus_tag="ACG8EF_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946293.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            6468..7031
                     /locus_tag="ACG8EF_000950"
     CDS             6468..7031
                     /locus_tag="ACG8EF_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4946294.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(7078..7593)
                     /locus_tag="ACG8EF_000951"
     CDS             complement(7078..7593)
                     /locus_tag="ACG8EF_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFS4946295.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(7619..8488)
                     /locus_tag="ACG8EF_000952"
     CDS             complement(7619..8488)
                     /locus_tag="ACG8EF_000952"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4946296.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8614..9294
                     /locus_tag="ACG8EF_000953"
     CDS             8614..9294
                     /locus_tag="ACG8EF_000953"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS4946297.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9447..10427
                     /gene="pta"
                     /locus_tag="ACG8EF_000954"
     CDS             9447..10427
                     /gene="pta"
                     /locus_tag="ACG8EF_000954"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS4946298.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            10550..11023
                     /gene="tsaE"
                     /locus_tag="ACG8EF_000955"
     CDS             10550..11023
                     /gene="tsaE"
                     /locus_tag="ACG8EF_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFS4946299.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            11016..11540
                     /locus_tag="ACG8EF_000956"
     CDS             11016..11540
                     /locus_tag="ACG8EF_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFS4946300.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(12030..12563)
                     /locus_tag="ACG8EF_000957"
     CDS             complement(12030..12563)
                     /locus_tag="ACG8EF_000957"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS4946301.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(13427..14179)
                     /locus_tag="ACG8EF_000958"
     CDS             complement(13427..14179)
                     /locus_tag="ACG8EF_000958"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFS4946302.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            14292..15212
                     /locus_tag="ACG8EF_000959"
     CDS             14292..15212
                     /locus_tag="ACG8EF_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4946303.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            15285..16220
                     /gene="murB"
                     /locus_tag="ACG8EF_000960"
     CDS             15285..16220
                     /gene="murB"
                     /locus_tag="ACG8EF_000960"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFS4946304.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            16518..17996
                     /locus_tag="ACG8EF_000961"
     CDS             16518..17996
                     /locus_tag="ACG8EF_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4946305.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            18273..20333
                     /locus_tag="ACG8EF_000962"
     CDS             18273..20333
                     /locus_tag="ACG8EF_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFS4946306.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            20416..20985
                     /locus_tag="ACG8EF_000963"
     CDS             20416..20985
                     /locus_tag="ACG8EF_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4946307.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(21160..21966)
                     /locus_tag="ACG8EF_000964"
     CDS             complement(21160..21966)
                     /locus_tag="ACG8EF_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946308.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            22104..23618
                     /locus_tag="ACG8EF_000965"
     CDS             22104..23618
                     /locus_tag="ACG8EF_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFS4946309.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(24021..25004)
                     /locus_tag="ACG8EF_000966"
     CDS             complement(24021..25004)
                     /locus_tag="ACG8EF_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFS4946310.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(25009..25656)
                     /locus_tag="ACG8EF_000967"
     CDS             complement(25009..25656)
                     /locus_tag="ACG8EF_000967"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4946311.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(25741..26373)
                     /locus_tag="ACG8EF_000968"
     CDS             complement(25741..26373)
                     /locus_tag="ACG8EF_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4946312.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(26389..26940)
                     /gene="coaC"
                     /locus_tag="ACG8EF_000969"
     CDS             complement(26389..26940)
                     /gene="coaC"
                     /locus_tag="ACG8EF_000969"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFS4946313.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(26937..27674)
                     /locus_tag="ACG8EF_000970"
     CDS             complement(26937..27674)
                     /locus_tag="ACG8EF_000970"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFS4946314.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(27671..28660)
                     /locus_tag="ACG8EF_000971"
     CDS             complement(27671..28660)
                     /locus_tag="ACG8EF_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS4946315.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            28918..29460
                     /locus_tag="ACG8EF_000972"
     CDS             28918..29460
                     /locus_tag="ACG8EF_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS4946316.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            29477..30562
                     /locus_tag="ACG8EF_000973"
     CDS             29477..30562
                     /locus_tag="ACG8EF_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4946317.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            30574..31380
                     /locus_tag="ACG8EF_000974"
     CDS             30574..31380
                     /locus_tag="ACG8EF_000974"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4946318.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLLLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            31381..32208
                     /locus_tag="ACG8EF_000975"
     CDS             31381..32208
                     /locus_tag="ACG8EF_000975"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4946319.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            32205..33278
                     /locus_tag="ACG8EF_000976"
     CDS             32205..33278
                     /locus_tag="ACG8EF_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4946320.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT        10974 a         5909 c         6835 g         9814 t
ORIGIN      
        1 cgtgagaaaa caactatatt ttttatgtgt tttgctttat aattaaaaaa atattgtcat
       61 tttatggagg aaaatatatg gaaaattatc ttagcctttt aggcgtcttg attgttattc
      121 tcgggtttgc atttaaatta gactcgatat tgatcgtaat ggttgcttta gttgttacag
      181 ctttgactgg cggtcttggc attcaaggaa tgttagaact tttaggtaca agttttgtaa
      241 acaatcgcgg gatggcgatt tttatcatca ttatgttggc aacaggaacg cttgaaagaa
      301 atggtctgaa agaatcagcg gcttccttga tcaagcgatt caagaaagta tctgctggta
      361 tgattgtaga tatttatggg atctttcgaa tgatctttgc ggcttttaac gtgagttttg
      421 gtggagttgc tggatttgtt cgtccaatca ttttaccgat ggcattgggg accatcgaat
      481 caaaagggtt agaagtccat cctaagcatg aagaagaact aaaaggtatg gcttcagcta
      541 tggaaaatgt ctgctggttc tttggccaag tactattcat cggaggagca ggggcgttgc
      601 tagtccaatc aacactgaaa gatctaggat atgacgtaac attgggacag cttgctttag
      661 tggaagttcc agtggctatc acggctttag ttgtggcaag tgtctacttc tatcttaaag
      721 aaaagaaatt agcgaagaaa tattatccag gaaaggggca ataagatgga cttttttaca
      781 aatcctgata ttttattagg cgataaatta ctggagatcc tttatatcgt gatgggactc
      841 gtattgattt acactggagt aagaaattta acagataaaa ccaatcctca tcgttatggg
      901 tcagcctttt tctggacggt cttaggggta gttattgcag gaggaagatg gcttccggca
      961 atcgtgaacg gtggcttgat ctttgcgatg accataccag caattgctaa gaaagtcagc
     1021 aaaggagaaa gccggcttcc atcaaaagaa tatatggaaa aaatgtcaga caaactagga
     1081 atgaaaattt ttattccagc tttgagtatc ggtgtctttg cgatcttgtt tgctctattt
     1141 accaatttag gtgctttagt cggcgtgggc gtcggtgttt ttgcagccat gctaatcttg
     1201 atgttctttt ctcgagataa tcgaccaagt acatttttag atgatgcagc agatatgctg
     1261 ggcacagtag ggccgttaag catgttgcca atgcttttag cttctctagg tgcggtattt
     1321 acaagcgctg gagttgggac agtgatttcc aatgcagtag gtacgatcgt tccagaagga
     1381 aatgtgaaca tcggcatcat catttacgct cttggcatgg tgatatttac ggtaatcatg
     1441 gggaatgcat ttgcggccat caccgtgatg acggttggga tcggtgcacc attcgtattt
     1501 agtcatggag cgaatccagc gttaatagga atggttgcat tgacttgcgg tttttgtggg
     1561 accctgctga caccgatggc tgctaacttc aatatcgtgc ctgtagcgat gctggagatg
     1621 aaagacaaat acggagtgat caaaaaccag ttgtttattg cgttatttat gctagttttc
     1681 caaattgctt atatgattct atttaaataa tagaagagag gggttgtgac agaataattg
     1741 tcgcaacctc ttctaacatt gtagtggaac actatttttt tataagaata tattaagttt
     1801 gatctgttat gttatttaaa ctattgtttc ctttatttac gctgttcaag gaaaatggta
     1861 tgataaaaga taaccatatg ttaaatcaat cggacggagg aacaacagat gaaagtacta
     1921 gtaacaggtt ttgatccatt tggtggggac aaagtgaatc cagcctatga agcagtaaaa
     1981 aaaatgccag atgaaatcag cggtgctgaa atcatcaaag tggaagtacc gaccgtattc
     2041 gaaaaaagca gtcaagtagt caaagaagcc atccaacagc accaaccaga tgtcgtgatc
     2101 tgtgtcggac aagcaggtgg gcggtcagca gtctcgttcg aacgtgtcgc aatcaatcta
     2161 gcagaagcaa gaatccctga taatgaagga aatcagccat tcgatacggc tttagaagaa
     2221 aacggtccag ccgcttactt tacctcattg ccaatcaaag caatgacaaa aaatgtacaa
     2281 gatcatggtc taccagcgta catctcttat acagcaggaa catttgtctg caatgatatc
     2341 atgtatcgtt tgcttcattt gatcgaaaca caattcccgg caattcgagg tgggttcatc
     2401 cacgtgccat tctcacccga tcaagtgatc gatcgtccag tgggtacgcc ttctatgtct
     2461 ttagaagaca ttgcttcttc tttgacttat gcagttgaag cagcagtaga aaataaagaa
     2521 gatatttccg gaaatgccgg aacaactcac taatttagca agaaacactg gctggttgct
     2581 aacgttatgt tagtagccag ctttttgtaa ttagaaagga aagaaacagc cgtggaagaa
     2641 tggcaatccg tctttgaaga atggtttcca aaagaaatca gcaaaagcta tcccatcaaa
     2701 atttctaaac aatacacaag cagtcaacgt tgggagatct atgcaaaatt aacgaaaaaa
     2761 cagcgggaac tagtcgataa gcaccgccga tatctaatca gctctcgttt tatggaagaa
     2821 cattatttag ctgcaacaga ttgggtgttt tctgacttca agatcaatcc gttttttcga
     2881 acaaaacgaa gccagcaaaa attgtattgt gagtgtggta gagaattaaa agtgcaatac
     2941 atcgttaaat cgccaaaaac cggaaagatc ctaaaactag gcatcaacca ctttgccgat
     3001 cacctgcatg tctctccgac agtggcagct tcgatccatc agggaatgac aaaagttgat
     3061 ctggccttag acgaattatt atggcttaag cagaagaata tcgattttcc tgaaggacta
     3121 tggcaaaaat actgttttgt cttgtaccaa aatcgtcgga tgaaacagcc atatttaccg
     3181 gatataaaat tagctcagcg gctcgcagaa tttcgccaag tagaaatgcc gatttacata
     3241 gcggattatc aagcattgga aaatgaaatc aagaaaataa gtgaacacat caatgggcag
     3301 tcaaaaaagc gacagataaa aaaagaacta tttgacgact ttgcagaaga attagtaaag
     3361 gatgtagaag aattcttgat caactatcgc gcatttttac gtaaagattg gcaatccatc
     3421 gtatatgaag aagtgccagt acatcctaat gcttattttg aaacatttat ttccgtgttg
     3481 agaaagacaa aacgtcaacg aacaccagaa gtcactgctc agatggaata ttttgcgaaa
     3541 aaccaacgat tcatccaacc aaaaatctat ctatttattt ggaagcaata ttgtcgctat
     3601 ggttttacag aagggttctt cgacagcatt cctcggatag tcagaaatgg ttttttaaaa
     3661 gtattgcgta aagaacgaga agctatacaa tctgctgata agaaagaccg tacagtctca
     3721 aaagaaaaat ggcaactagt agtgaaagat atccaatctg gaaatgtgca agagacaatc
     3781 gacaaatgga aaggaaaaca ttatcgtttt actgaagctc agaaacaagc gttggaatat
     3841 tatcagaagt tagaagaatc actgcgtttc aatgatgagg caagaaagta tctgaaggaa
     3901 ttactataaa aaatagaggc tgggacagaa gcgtttaact ccaagaaata agaagaaatt
     3961 cacgaaaatt gcttttcaaa tttttgtgaa tttcagctta tttccgaagg agttgcttct
     4021 gattccgccg tttatacgtg tttagagcgt gagacaaaag tgttctttac ttttgtccca
     4081 cgctcagttt ttttatgcgt gttgttgtgc gttgtagtat tcttcttcca ttttttgcat
     4141 acgttcacgt tctttttggt tataccaagg tgaaacggta aatgcaagga catcattcag
     4201 caagtaaaca gagctgttca cgaacatcgc caaacttgca gatccatgcg taaatgaaat
     4261 ataccaaagg accatttgag caagccctga agctaaccac cagtaatatt ggttgttgta
     4321 acgcaagaaa ctgatgatac cagcagtcaa gctaatagaa aaagcaatgg catctaccca
     4381 cggccgagga tcatctgtat aacgaccaat caggaatcct gaaacaagat agacaaggaa
     4441 agtactgatg attgcgataa accagctttt tccagtgaat tttcggattt ttgaagccat
     4501 attatgattc cattctttac ttaacaatac ggggatatcc aatgtgatca tataagcaat
     4561 ctgttcaccg atacttaaat agttctttgc tgaaaaccca acgataataa aacaaatcgc
     4621 agacaaaata cctaaaatac cgttgactga ttttgccgca ttgatggata aaatacacaa
     4681 tacaccaagc gtggtaccga taaatgtaat aacggacagc caagtgatgg gactattgac
     4741 aagagtcatg acctggcagc caagactgaa aaagaaaaga taataatttt gttgcggcca
     4801 gccttttagc tgatgagcta aaaactgaaa atatgacttc atgtatgagg actccttaag
     4861 acactaaatg tagtgtgttc tgtatttact aacacaatct attgtatgct tttcttgaaa
     4921 aaaaactagt ctttcaatta taaataattt gtgttatgat aattttctgt ttgttttctt
     4981 gaaaatataa tttcttactt tcatttatta tattgggaac aggaagagtg tcttggagga
     5041 cctactttct gttttttttg ccttttttct tataaaaagg aaaaacagct atttttttca
     5101 atctgacaca tttacaatgg aactggagat taatatagat ccgagcttaa acgaagaata
     5161 ggagtgctca tatgaacctt acaacgatcc atcatatagc catcattgtt tcagattatc
     5221 agaaatcacg tgatttttat gttaataaat taggatttga agtaatcaga gaaaattacc
     5281 gcaaagaacg agatgactat aaattagatc tgaaactagg aagtgctgaa ttggagattt
     5341 ttggaattgc taatgcgcca caacgaccaa actatccaga agcttgtggc ttgcgtcatc
     5401 ttgctttcaa agtagacgat attgaagaaa caattgcaga attgcagtcc ttagggatcg
     5461 aatcagaacc gattagagaa gataccttta caggaaaaaa aatgacgttt ttctttgatc
     5521 cagacggatt gccattagaa ctgcacgaat aaagataaca aaattgtcac ttttttttcg
     5581 tttcattcga tttttcattc gttcatttct tgatattctt taaaaaaaag aaacaggaga
     5641 atgaagaatg aaaaaaatga tgtttggtgt tgctgccctc atcgtaatag ggattaccgg
     5701 tatgacggga ctgctcaatg ccgcttttca catggatgat attagtgacc aggaatatcg
     5761 gatctcattg aaaaaagcag cagcaatcgt aaaaaacgaa acaaaagaac ataacatatc
     5821 tgctattcat tttgcagttg aaaaagattt ggagaatcct acgaatgcaa catatgaata
     5881 ccagtttttt tgtgaaggac aagtcatagc agtcaatcca tccactggga aaacaaccgt
     5941 acgaaatgac acggaaaagg ccgcagaaca tctttttgat atagaaaaaa cggaagagtt
     6001 caaacaaccg caagcagcga tgaaggaagc agtcagctac tacgggaaaa aagcaaaagt
     6061 gaaagagtgg acacttctag tcaaagatga acgtccttat tataaagtcc gattaataga
     6121 tggagaacag gaccgtcagg tatggatatc tgcatagaag aaggatattg cttctctttt
     6181 ttacaatata agagtaagct aagagagtaa agatgaaagg aagttgagat aacatggaaa
     6241 aaatgaaatg ccctaattgc ggaaaaaaat ttgcatacga agaagtcaat aatgtagtcg
     6301 aacataatga taaagaaatg ccagtcgtct gcccttattg ccgaactgaa gcggcaagaa
     6361 tcgttactca tggttatttc attactgaaa agattgaaga ttttttaaaa taacgatttt
     6421 gcgatttctt tttctgcatg ttagaatgga gaaaaggaga ggaaactatg attcgttttg
     6481 cgagaaaaga agatgctcca gaaatcgccc cattgatctt agtgattcta aaagatatgg
     6541 aactgccatt tttgaagcag acaggggaac aaaagacttt agagatttta gaagaagcaa
     6601 tcactgatcc aaattatcgt tacagttatt cacgaggtat cgtagaagaa atagacggta
     6661 aagttgcagg agttgcttac ggttatacag atgaagaaga atcaatcatc gatcagccac
     6721 tggtacctat cttagagaaa tatcagctag atgcgtctac acgtttgttc attgataaag
     6781 aaacgtttcc caacgaatgg tatttggact ctatcagtgt atcagaagat tttagaggac
     6841 aaggcatcgg ttcgcgatta ttagaagcat tacctaaatt ggcgaagaaa gctaatcgct
     6901 ccgtcatcgg tctaagtgtg gatgaaaaaa atccaaaggc taaaaaatta tacgaacgcc
     6961 atgacttcaa agttgtcggt cagcggatga tcagtggtca tctttatgat catatgcaaa
     7021 agaatatcta aaaagatgta cctgaaatgc gtatttccgc atttcaggta catcttttta
     7081 acttaagtta agtcggtaag aaacaggctt atcgcgactt ttgtctttac ggtagagcca
     7141 gtaatctaaa accgcagcaa tacaaataca aactggagca tcttcatcat ttgagacatc
     7201 caatacatag taatctcctg aaaaaagtgt tgctttatcc attttcatga tttgctgatt
     7261 gaaatgatgg atttgatatt gatgattata gatatcacct tggactgtcc agtgaagtct
     7321 acgaatataa taaaagtcac cgggccaatt gaatattttc tgcatggttc ccaccttttc
     7381 gaactcttta tacagctcaa aacgcgtacc gaatgtcagg cttatctgct tgatgtgagc
     7441 aacgagatcg ccattcatgg cataaagaga caatgcatcg ccctttgttc cccagcgacc
     7501 aaccatcaaa aacagggaat gtcctttttc gtttttgaca attgtccgag ttgcacgtcc
     7561 aagctgcttt tcctgaatga aaaattctga catctatatc cctcttttct agataaaact
     7621 acaagtcttc tgcaatcgcg cgcaatatgg cttttcctac gccagattca aggtttgtgt
     7681 ccgttgtata tttggcataa tcttttactt cgatttctgc atttcccatt gcgaaactga
     7741 ctcctgctac ttgcagcatg ctgacatcat tgaagttatc gccaatcgtc atgacttctt
     7801 ctaaagaaat cccacgctcg tgagcgacat gggcaacagc aattcctttt tgagcgtttt
     7861 tatggttcac ttcgatattg ttttgaccag aggaagtaac ggctagatcg cctaaaagag
     7921 aaatctgctt tccaactgga cctaatacag ctggcccttc tgtatgaaaa caaataattt
     7981 ttaaaacttc cagattcttt tcttccaaaa tagcacgaat actgtccacg tatgtgatgt
     8041 gcaataactc cagcttagct gaggccatgg caatcgccat tttatacgta aggtgaggta
     8101 aatgggtagc caccatggaa gcaaaacttt caattcgttt cgcttgattt tcagaataaa
     8161 gaccatcgtt ggttgctact tcatagtaaa taccgttagc atctagaata tctaaaacag
     8221 agattgcttg attatttgga atcgacacgg taaacaatga atttccttct ttatcaaata
     8281 cctgtgcccc gttcaaagtg atcatcgcac aatcgattcc tacctcatct aaagctgcgc
     8341 gagcttcttg atagtttcga ccagtagcga ccatgaactc aacaccggca tcatttgcaa
     8401 aacgtatcgc ctcggcattt tcaggagaaa cactcatatg ggcatctaat aatgtgccgt
     8461 ccatatctga tgcaattaat ttgatcataa tgattatcgt tcctttctgc ttcttgttcc
     8521 tagtttacca tattaatcaa gtgctgaggg tgaagattgt cccttgtgat tgaagaaaat
     8581 gtgagttatt ctttttatta ggaggtggag tttttgaaag aaatcattca taacagttgg
     8641 caagaagttt tgtcctcaga attttcaaaa gattattatc tgcatctaag agaattttta
     8701 aaaaaagaat atgcgtccca aaagatccat ccagatatgt atcatatcta tgaagcattg
     8761 gaattgactc cttatgaaga agttaaagtg gtgattttag gacaagatcc ttatcatgga
     8821 gaaaatcaag ctcatggctt atctttttct gtgcaacctg gcgtaaagat cccgccatcg
     8881 ttaagaaata tctataaaga gctttatgat gatcttggaa tcgcacctgt tcaacatgga
     8941 aatctagtgt catgggctaa acagggagtc cttttactca acaccgttct taccgttcgg
     9001 gaagggcaag cgtattctca tcgcgggaaa ggttgggaaa gattaacaga cacgatcatt
     9061 gaaaaattaa atgaacggga aaaaccaatc gtgtttatct tgtggggaaa accggcacaa
     9121 gaaaaaatca aaatgatcga taagagtcgc catatcatca taacatctcc acatcccagt
     9181 ccgttatctg caagtcgtgg cttttttggt tccaaaccct tctcgaaaac gaatgatgcg
     9241 ctccttgcgt taggagaaga accgattgat tggcaactgc ctgaaacagt gtaataaaac
     9301 agcgctttcc tgttatagtg cagttcgctt tgtatgtgaa aaacttaatt tttccagcat
     9361 ttttgactga tacagataaa ataaactgtt ctttttttct ggaatagtgt atgataatac
     9421 atataaaaac gtctggaggt catcttgtgg aattgtttga tagtttaaga tttaaaattg
     9481 ttcgccgtgg gatcaaaatc gttttcccgg aagctactga tgctcgtatt ttaggcgcag
     9541 ctgcccggtt gaaagcagaa gaacttgtgg aacccatttt agtagggaac gaagaagaaa
     9601 taagaaatgc agcgcatgcc agaggaatca aaacatctag ctttacgatc atcagccctg
     9661 ataattacga taagtgggat gaaatggtgg atacttttgt tgaacgtaga aatggaaagg
     9721 catccaaaga acaagcagaa aagattttaa aagatgtgaa ttactttggt acgatgctta
     9781 cgtatatggg gattgcagac ggaatggtct ctggagcgat ccattcgact ggcgacactg
     9841 ttcgcccagc acttcagatc atcaaaacaa agcctggcat cagtcggaca agcggagcga
     9901 tgatcatggt acgtggaaga gatcaagaaa aatatgtctt ttcggattgt gcgatcaacg
     9961 tgaatccaac tgcgcaagaa ttggctgaaa tcgccgtaga ttctgcaaaa acagcagaat
    10021 tgttcgatat cgaaccaaaa gtagcaatga tgagcttctc gacaaaaggc tcagcgaaat
    10081 cgccagaagt agacaaagta gttgaagcaa ctaaaatcgc taaaaaacta gcgccacaat
    10141 acatgatcga cggcgaattg caatttgatg cttcttatgt tccttcagtt gcacaactga
    10201 aagcgccaga ttcagaagta gctggtcaag caacagtctt tgtatttcca gaattgcagt
    10261 caggaaatat cggctataaa attgctcagc gtttcggtaa ctttgaagca atcggaccaa
    10321 ttctacaagg cttgaacaaa ccggtttctg atttgtctcg tggagcaaat gaagaagatg
    10381 tatataaatt atcgattatc acagcagcac aaacattgat tgattaacaa atacacgcga
    10441 actagccaaa aacagcagct gctgttcttg actagttcat tttttcttta aaaaaacttc
    10501 attacgcgat atactggcaa aaagaaagaa gagcgggaga agatgaaaaa tgatcgaact
    10561 atcaggatta acaatgacag aacaattcgc agaggcaatt ggtcattccg caatgccagg
    10621 tgacaatctg atattgactg gtgatctagg tgcgggtaaa acaacgttga cgaaaggaat
    10681 tgcccaagga ttagggataa cgcaaatgat caaaagtccg acatacacga ttattagaga
    10741 atatagtcaa ggtcggattc cgttgtatca tatggatatt tatcgggtag cagctagcgg
    10801 agcagattta gggttagatg aatattttga aggtgatggg ctttccgttg ttgaatgggg
    10861 aaatctatta gaagaagcac tgccggagga ttatttggaa ctgattttag aaaaaagtga
    10921 tacagattta gaaaagcggt atgtgaaact aatctcatac ggggagcaaa gtgaaacctt
    10981 caaacagcga attctagaaa aatgggctga aaatcatgag tgaagaagtt gggatcatct
    11041 tgcgggaagc agtgccagga gatgcaaaag acattctttt gatgatggga caagttaata
    11101 aggagacaga atttttagtt ttggatgaag cagaactcct gttaccacca gaaaccttag
    11161 aagaagagtt ggattatata tacgaaagca ataataattt attgctgctt gcaatctatg
    11221 aagggacgat tattggaaca gcatctgtca aggcagacag tcaatttcgt ttgtctcacg
    11281 tgggagaagt cggtatcagt attctgcagg aatattgggg aatgggacta ggcactctta
    11341 tgctagaaga aatcatcagt tgggcgaagg aaatgggtgt actttttcga ttggagcttg
    11401 acgttcaagt tcgtaatgaa agagccgtac acctttaccg aaaaatgggc ttccaaatcg
    11461 aagcggtcat gcctagaggg gcaagaacag atctaggtga atttttagat gtatataaaa
    11521 tgagttattt gattgaatag aagaaatagg aaaagagtga gaaactgtca tccttttgag
    11581 aaggcagaaa aacacacgaa gggactgtga caaaagtcgt gtcgcagtcc attttttttg
    11641 aataaacggt gttcaatcag ctgaccttcg gtattagctc aaaaaacggc aaaatatgag
    11701 gaactatttt gccgtttgtt tctctaatac tcaggtcaaa acgctgattt cacaacctct
    11761 tggtaaacgg tgtccaaaag cttgcatctg cggtaataag tccaaccaaa tgaaaaatat
    11821 gagaagctat ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc
    11881 tctccaataa acggtgttct cctgccagct cctcggtcac cagtcacaat atttaataaa
    11941 catgagaagc tattttttga aattgctcct tgtgactcgg agtgaaacgg caggctcacg
    12001 acctctttgt gtgtttcata agtattcttt tactttatag tcacaagttt tttcaatgga
    12061 tctgtcccga attttttttc ttgataaagc aggatttccg cacatgcacg actatcttcc
    12121 aatgcatcat ggtgatgatc taattggatg ttcaaatttt cgcacacagt gttcaatcga
    12181 tgatttggaa attcaggaaa caggcgacgg ctacttttca ctgtacataa agaaagataa
    12241 ttaggctgtt caagatgata ataatctaag catcccgcta agacctttgt atcaaaagca
    12301 gcattatgtg caacgactaa acgatttttt tgaaaatatg gttggatagt ttgccagact
    12361 tctgggaatt tcggcgcatt gcgtacgtct tcttgatgga ttccgtgaat ttggacattt
    12421 cgccagaaaa aaggagtctc aggctgtatc aatgtataga attcatcaac gatctggcta
    12481 ttttttacca tgactagcgc aagagaacaa gcactataag gctgatgatt ggcagtttca
    12541 aaatccatgg caacaaaatt catttctgta aaacctcctt gggaaattac tgtaacatac
    12601 ttttgaaaaa agcaaataaa tttaacaaaa aacatagaat tttttatgta atatttgttg
    12661 agaaaagcta gtttttgaac tcattccttg tgatttggag ttagatgggg atcatgactt
    12721 ctcggtatta agtcaaattt atgcaaagtt gtgaggaacc attttcaaaa aaatttctta
    12781 cgactcgaaa gtaggcgact ttttcacaac ttctccacta aacggtgttc aatcagctga
    12841 ccctcggtat tagctcaaaa aatagcaaaa tatgaggaac tattttgcca tttgtttctc
    12901 taatactcag gtcaggacgc tgatttcaca acttctcaat aaacggtgtt caaaagcttg
    12961 catctgcggt aataagtcca accaaacgaa aaatatgagg aactattttt gtttggctgt
    13021 tcttattact tgatgcagtg cagctttttc acaacctctt aataaacggt gttcaaaagc
    13081 ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg aggaactatt tttgtttggc
    13141 tgttcttatt acttgatgca gtgcagcttt ttcacaacct cttaataaac ggtgttcaaa
    13201 agcttgtatc tgcggtaata agcccaacca aacgaaaaat atgaggaact atttttgttt
    13261 ggctgttctt attacttgat gcaggacagc tttttcacaa tctctcgata aacagtgttc
    13321 aaaagccagt ttctcggtat tatgccaaat ttatgcaaaa tgtggaaagc cattttcaca
    13381 aaatttttct taatactcga aactaaacga ctttttcaca acctctttat ttcaaatcta
    13441 attctactgg acaatgatca ctgcccataa tgtccgtatg gatctttgca tcagctagtc
    13501 ctggctctaa atctttgctc gtcaaaaagt aatcgatccg ccaaccagca ttgtttttcc
    13561 ttgaattgaa tcggtagttc caccaagagt agacaccttc ctgggttgga taaaaataac
    13621 ggaatgtatc gataaacccg ttatccaaca gtcgagaaag agccgctctt tcttctggag
    13681 tgaatccagc atttttttga ttcgttttcc agtttttcaa atcaatcttt tgatgagcga
    13741 catttagatc cccacacacg atgactggtt tttgtttttt tagattttcc aaataattat
    13801 aaaaggcttc ttcccattct aatcggtaat ctaaacgttt taattcgctt tgagaatttg
    13861 gcgtgtaaca ggttacaaga aagaaatcgg aatattccaa agtaatcaga cggccttctg
    13921 tatcatggat atcgataccc attccgtaag tggcattcaa tgctggtttt ttagcaaaaa
    13981 tcgctgtgcc ggaatagcct tttcgttccg cgtaattcca atattgataa taacctggaa
    14041 gatctaaatc aatttgtcct gcttggagtt tcgtttcctg cagacagaaa aaatcggcgt
    14101 ctaactcatg aaaaacttct aaaaaatttt tatttacgat ggcacgaagc ccgtttacgt
    14161 tccaagagat aaatttcaat aaaagcacac tccttcgaat taatttcctt acctttgcga
    14221 tactattgta cctcatttta gtaaaaaatg gtgtaagatg aaagtaacta aatgatccag
    14281 gaggaaagta gatgaagatt ggtgtaatcg gtgtagggaa tatcgcagaa aaagcatatt
    14341 taccaactta tgcgaaaaaa caaggaacac ttgattttta ttttgcaaca agaaataaag
    14401 aaacaaaaga aagaattcaa aaagagtacg ggttccaaca tctatatgaa acgatcgatg
    14461 agcttttaga agaaaaaatc gaagcatgta tgatccacgc agcaacaaaa gtacattatg
    14521 aattagccaa aaaatgtcta gaaaatggta tccatgttta tattgacaaa ccactgagtg
    14581 tccatctaaa tgaagttcgt gaattgcaag aattagctga taagaaccaa ataattttga
    14641 tgatcggttt caaccggcgc tttgctccta tggtagaaga attgaaacgt atcccagata
    14701 agcggttgat ccacctgcaa aaaaatcgaa tagcggcaaa agaaacgaca gagttcgtcc
    14761 tttatgattt gtttttacat cttattgata cagctgtgta tttattagat gaacccattt
    14821 tgagaacaaa ataccagata agagaaacaa aggaattttt ggaatatgcc attttacaat
    14881 tagaaacaca aaatcaaacg gctattttaa cgatggattt agcaagtggc gctaatatag
    14941 aaaaatatca agtaacaagt aaaaatggta cttatgaagt aaacgatctg acccagcttg
    15001 tcattcaaga ggctgatgga aaaaaagtga aagaatttgg cgactggaca aacacgctag
    15061 tcaaacgcgg gtttgagcca atggtagagg ccttcttttc tcagatccgt ttaggtaaat
    15121 cggaaacaga attgcttaaa caaaaagaag ttgtaagaag tcatgaatta tgtgaggaag
    15181 tgcttaaaga tcactaccgt catcaacttt gaacaagact ggcaagattg tattaagcgt
    15241 gatagaataa tgaggacgtt tgagaaaaga aaggaaaatc ctcagtgaat aaagaagaaa
    15301 tagttgaaag cttagccacg attactatat tgtttgatga accattgatg aactatacgt
    15361 ttacgaaaac aggtggacca gctgatgtcc ttgcattccc gaaaaaacaa gaagaagtaa
    15421 agcaaatcat cgattattgc cgaatccatg atataccttg gatggtatta ggaaatgcaa
    15481 gcaatctgat cgttcaagat ggcgggattc gcggtgtagt catcatgctg actgaaatga
    15541 aacagattca tgtcaaagga acgatggtca ttgcggaagc aggtgcctca ctgattgata
    15601 cgacttatgc agctttggca gaatcattga ctggatttga atttgcttgc ggcatcccgg
    15661 gaagtgtcgg tggagcggtt tatatgaatg caggtgcgta tggcggagag atcaaagatg
    15721 tttttgctga agtggatctt ttgcttgaag atggaacatt gaaaactttg acaaaagacg
    15781 aaatggtatt ttcttatcgt cacagtaaag tccaagaatt acgtgctatc gtattggaag
    15841 ctcgattcag tttgcaaaca ggggattatg aagcaatcaa agcaagaatg gatgagttga
    15901 ccgaattgcg tcaatcaaaa cagccattag aatacccttc atgcggaagt gtctttaagc
    15961 gcccagtagg acactataca ggtcagctga tccaacaagc aggattgcaa ggcctgaaat
    16021 ggggcggtgc acaagtctct gaaaaacatg ctggattcat cgtcaatatc gatcatgcga
    16081 cagcgacaga ttatattgaa ttgattgcgc acatccaaga agtaatcaaa gaaaaatttg
    16141 atgtgtcttt agaaacagaa gtgcggatca ttggagaaaa acaagcaata tcagaaacaa
    16201 gcagaagttt agccaattaa taagtaatgg aacttaaaat aagtaaaaga gcaggacggt
    16261 ggccgtcctg ctcttttttg tggttgttta cgcaaatggc gcaaatcttg tacattttaa
    16321 cacaataaaa caaaaaatat agaatatttc ctcataaatt tttccatttt ttgcaatatt
    16381 ctcaaaaaaa agataaaaaa tgagaataac tgatgaaaaa acaagcataa tccatcataa
    16441 aatcagtata ggtgaaaaga tgaactttca tctgcatatc aagtaattgg tatgaggcat
    16501 tttttgaggt gtaggatatg aagttggatt accttataga agaggaagat agagaaaaac
    16561 tgctgctagc tagatattta gaatttgaga aaaagaatta tgtagtgctg cctaagttat
    16621 atgaaacatt aggtttgtct aaatttaaaa caagaaatta tatcatagaa ttaaataaag
    16681 aatttactga attcaaaaca aagccagaga tccaaatttc agaagaagca gagatccatg
    16741 tcgaacgtct gaatttgacg gatatcaaaa atttaagaag aatctacttt caacgatcaa
    16801 aaattgctcg actgttaacg gagatcgtgg attcaaaagt aacgatcgaa aaatttgcag
    16861 agaatcattt tttaagcata tcaagagcct atgtaaaaag aagagaatta gtctcttttc
    16921 ttgaacagca tcaaataaag ttaaagaaaa acatattagt tggggaagaa ataaacatac
    16981 ggaacgtatt atttggggta tatttcgggg tctttaatgg tttttctatg ccatttagag
    17041 aaagcgtcat gaatgaagca aagagtatcg tgcaatattt tgaatatctg ttcaatttaa
    17101 aactgtctgc aacaaaacga atcaaattca atttgctaac cactattagt ttgttgaggg
    17161 cctatgggaa tcaaccaatc ggaaaacgtt tcttttcaga tgaatacttt aagagctcag
    17221 aaggaatgaa gatcatcaag gatttatcac atatttcttt tcttcatatc cctaaagagc
    17281 agatcattga tgagatttct tatatcatgt tgtttttgat cacggaagaa gaatttgaat
    17341 tagatggcgc atttggactt gcggaagctc attttcatga tatccacatg attagtcaaa
    17401 aaatgtcagt ggaattgatt gatcaattac cattttcgat tgaaatctct gaagaagaaa
    17461 gactatatat acgaaaagaa cttcgtatgg gtatgatgca tgtcaatcga aaaaattgga
    17521 tattcgactt tgagatgact agctttacgt ctaaaaagca gctacagttt ttcttcgaga
    17581 cgtatccaat attcagtaca gcaatccaaa aggtcataca agagaataaa cgctatttct
    17641 tgggaaaaga tgaacgtttc atgattcgct tattttatga ttatctcttt ttattggtcg
    17701 gtatttgtcc ggtatcatat tttgaagcac cgattcatgt ctgtattgat ttttcgcaag
    17761 gatctgctta taccgattac attatttctc aagtgaaagg atttaaaaat tacaatattg
    17821 tgattgagag tcgaatcact tctcgtacga atatttattt atctgattgt ttacttgaaa
    17881 aatttaataa agaacagata atttggaaaa atccaccgac tccagatgac tgggaatatt
    17941 ttggaaatac catcatcaga gaaaaaaata gacatttaac tgaaaaaatc agctagtatt
    18001 tatggatggc agctttgtct cacatattta ttagctgatt tatgtttttt tcaaagaaaa
    18061 ggtaactaga tataactaga tataactaga tatattactt aacaaaatta aaaagtacat
    18121 aagttattat tccgattttc ttgaaacaat aaaaaatcaa aaaaaatact ctcctaatag
    18181 tccatcttct tttgatttta tttatataat gaaatcatct tatgaaaatt actacttcat
    18241 gtttagacaa gagtagggag gactaagaat aaatgtgttc aaaaagacgt tcaaaacaac
    18301 taaataaaca cgcttgtcgt ttagataagg aacggtcgtg ttctgaaaaa cactacgtta
    18361 agtttatgat gcgcacgatg ctgatcagtt tatctgtagg tgtctatatg catgtacccg
    18421 ttcaggcaac gacacaaacc atcaaggtcg tacgtacctc tgtttctgag accactgatt
    18481 cccctatcac tattactcat actttcactg atgcaaatgg aaaacagcta cttcctgcat
    18541 tgacttccta taataaagtc agttatcctc caacgataaa ccactactgg ttaattccta
    18601 acaaaacatt atggacatcg gaaaccggaa gcagtgtgcc gctttctatc ttttccgata
    18661 tgactgatcc tacaaaaggt gatcagttaa aacagatcac tgatattttg aatcatgctt
    18721 tgacagatat aaaggacggt ggaagcgcac aagtttctta tgtatatgaa aaagattatt
    18781 cacagtttga aagaaagaaa atcgatgttc tggcagggac aaaagtttcg atatctgatc
    18841 tggtagaaag gctgaagaac gcagatggaa cacaaggaga tatgtccaaa gtaatttcaa
    18901 acaatgggaa tatcgtggat acgactaata caggaacatc tactatttcc ttgtcctatt
    18961 ttgatccaat tgctttaaaa acaatgactg catctgctgt gatccatgtt cgtgctgatc
    19021 aaacagctat caaaggaaaa gatcttcact taagaacagg agagagttac gcccctaaag
    19081 atcttgttga atatgtaaca aaaagcgatg gaacttcagg aaataaagaa gaaatcaaaa
    19141 tgaacgggac agtagatact acaactccgg gagaatatcc cgttgtatta acttatatag
    19201 actcgaaaac atcaaatgtg gcagaaacaa cagctgtagt ttatgtagaa gcatctgctg
    19261 cttcatcgac tgtcatcact cattttatag atagtcaaac aaataaagaa cttcaagcag
    19321 aaacacatat gagtagtgta aatcgtattc ctgatttgcg cctacctgct gaattttctg
    19381 atcgagtgcc aaatatagat ttatcgactt atgaacaaaa tgggaaagtc acttctattt
    19441 ctgattggat gcaagcaaac ggattagaca gaaaaagtca ctggtcggag atccttgaag
    19501 tacttcactc agaactgatg aataatagga agcaatcagg aggggaaatt acctttactt
    19561 atgtttatat accagacaat cgttcattag aaggtcatga tcttattgtt catccaaacg
    19621 aaaaaataaa caaagaagag ttgatccaat cagcaacaaa ttccttaggt aaacccgtac
    19681 cgttaaagga tgtatcggtt acaatcaatg aagtaccatt ggaggatggt tatttgacca
    19741 aacaaacagg tgattttacg attatgtata gttatcatga cccgtattca ctccaagaac
    19801 tccatgctca gtcactatta catgttttac cattaaacaa acagatagat caaaaagaac
    19861 aaaaaagacg agaagaaagc ttgtcagaaa aaaaacaaca aatggctcat gcagtttctc
    19921 caacagggaa agtaaaacaa acaagaagcc tagagttagc aagaatatct agaaaaatct
    19981 cagaatctaa taaaacgata aggcaagaag ctcatttggc cccacaagtt tctggattgg
    20041 aaaatgaaat tttagagcct aaaataaaac aacaatcaga aaaagaattt gttgctaaac
    20101 agtcggaaga tcagaaggaa tcagatgacc agaaggaaac gaagaaaaaa cgcttgtcaa
    20161 ctattgattc caccgatgag catctgcagc cgcaagtacc acttcctagt ccgggaacac
    20221 ccgcacctgg tggaggtatt ccttcttctg ctttaggaga tttcatgcga ggtatggcag
    20281 gaacatgggt atacgctaat cgaaatgaat ttgacgcaga tgactggttc tgatttatat
    20341 ctctatctgt tatagtcaaa taagtagctg agtcatatgt tatttggcat aatggagacg
    20401 aggagggata gaaaaatgtt agataaaacg ttaccttacg cagaaatctg gatgacaaga
    20461 ccacttcaac aaaatgttcc aatcacgcct ttagcggaag gttttgaatt tcgattttat
    20521 caaaaagaag atgaatctgc ttgggcggag attgagactt ccgttgggga atttcaaacg
    20581 acagatgaag cacttgagta ttttcaacaa aactttgctc cttatcctga agaacttgaa
    20641 agaaggatgt tttttgtaga gacaaaaaaa ggagaaaaag tagcaacctg taccgcttgg
    20701 cgaaaaaaat caagagagca agtgtatccg gtttttcatt ggttagcagt gaaaccagat
    20761 taccaaggaa aagggctcgc aaaagcattg actgccagag tcttgcaaga atttcctacc
    20821 ctccatacgg acggaccaat ctatcttcac acacagacat ggagccatca ggcgattgct
    20881 ctttataaaa aaatgggttt tacttttatt gctgaaaatc tagacggcac acctaatcca
    20941 gattatgaaa aagttatgca ggttttaagt aaattagata aataacgaaa tggtgaaata
    21001 agtcctcagt attgggataa aaaacgaaat agtcattcct catatcttac ttttttgagt
    21061 taatactatc ggataactaa tggcatgcca tacattcaaa aaagaagggg cagtgacaaa
    21121 agtattgtca ctgccccttc tcttgttttt tagcatgttt tatcgtttga tttttttagg
    21181 aataaaattc ttgatgaacg taaatttttg gtaatcgttt cgactataga ggtaaccaaa
    21241 tacactgaaa acaactgctc caataaaatt aacaaataaa tccttcatag tatccatgat
    21301 tcccacgtct aagtaaccat tttggatgat tgttttatct gttttaccat ctgcattttg
    21361 actttcaatg attgtccgag tgatatctgg aacatgatag acagtgtttc cgtctttact
    21421 taactccaca ctattgattt gctggatgat cgtatctttt tgcatatcga attgcagtaa
    21481 tttatctgct gaaaattcca caaactccca cataacacca actgtcatcg aaaaacagaa
    21541 ggaaacaact gctagtaata aaggagtcaa gttcatggtt tttgtatttt tattgagcaa
    21601 ataaacaagg gaaaagccga cacccccagc taaaaatccg ctgaatgcat gtagaaaagt
    21661 atcccacatt gaaaaatagc cataaaaatc acttaactct cctaaaactg ttgaactaaa
    21721 aataaaagat aacaaaatga tttctagcat atttggaatc tgtatcttta atatcttttc
    21781 cagaaaataa gggatttcaa ataaaattaa tgtcagtaat aaaagcattg tatttcccca
    21841 gtgttggttc cagacctgat ccactagcat aataacgacg atgatacgta ataaaatata
    21901 gattccgttt gtgattctac ctgcagtttt tgtactgttc gtaaaaaagg ttttcaaatg
    21961 attcataaaa taactccttc atgtatatcc ttaattatac taagaagcgt gtgtttacca
    22021 aagctatatg acttaaagaa aaacagagtt gatattgtga aaaaaatcac ttaatatagt
    22081 ataaggagaa aagggggata caaatgaaaa aattagttgc tggtttatta acagccgttg
    22141 cacttcttgc tatgagcgca tgcggcgcag atcagaaaaa tacagcgcag acaagttcta
    22201 gtacgaagga atctgccgaa gtatcatcag gcgcttctga acaagtatat acagatccaa
    22261 aagaaatgaa agaatcttat gatgtcgtga ttattggagc tggtggtgca gggatggcag
    22321 cagctatcga agcaaaagat gctgggttga atccagtaat tttggaaaag atgcctgttg
    22381 ccggaggaaa cacatctaaa tcatcaagcg gtatgaatgc ttcagaaaca aaatttcaaa
    22441 aagaagaagg aatcaaagac tcgaatgatg ccttctatga agaaacgcta aaaggtggtg
    22501 gaggaacgaa tgataaagaa atgcttcgat actttgtcga tcactctgct gatgcggtcg
    22561 attggctaga tcaaaacggt atcaaactgg acaacttaac gatcactgga gggatgagtg
    22621 aaaagaggac acatcgtccg tctgatggct cggctattgg tggatactta gtggaggggt
    22681 tattgaataa cgtccatgag cgggaaatcc ctatttttgt caacgcagat gtgacagaca
    22741 ttaagaaaaa tggggaaacg gtcaatgaag tgactgtaca tgttcaagga gaagaaccaa
    22801 aagaggtcac cggaaaagca atcgtagtta ccacaggcgg ttttggtgcc agcaaagaat
    22861 ttattgaaaa atatcgtccc gatttagctg attatattac gacaaatcaa gaaggatcaa
    22921 ctggagatgg tatcaccatg attgaaaaat taggtggaca aaccgttgat atggataaaa
    22981 tacaaattca tccaactgtg cagcaggatg agggggtctt gattggtgag gttgttcgcg
    23041 gagaaggagc aatcttggtc gatcaagagg ggaaaagatt tgtcaatgaa atggatacaa
    23101 gggataaagt atctgcagca attacagcat tgcctgaaaa atcagcttat ttgatttttg
    23161 accaaggcgt gcgtgatcgt gccacagcta ttgaatttta tgaccaaaaa ggatatgtca
    23221 caactggaaa gacaatggaa gaattagcag aaaaaatcga tttaccaaaa gaaacactag
    23281 caaaaacagt tgatggatgg aatacggatg ttgcggagaa aaaagatgct caattcaacc
    23341 gtgccacagc catggaacat gacttatcgt ctccgaatta ttatgcgatc aaaatcgcac
    23401 caggaatcca ttacacgatg ggcggtgtga aaatcaatac gaacactgaa gtattagata
    23461 aagaaaacca gccaattagg gggttatatg ctgctggtga agtcacaggt ggcttacatg
    23521 gaaacaaccg gatcggtgga aattcagttg cggagatcat tatttttggg cgacaagcgg
    23581 gcatacaagc agctcgattt gcttcggaaa atgaatgaaa tagaacgtaa ataaaaagag
    23641 gttgtgaaat cagcgttttg agctaatacc gagggtcagc tgattgaaca ctgtttagtg
    23701 aagaggttgt gaaaaagctg ccctgcatca agtaataaga acagccaagt aaaaacagct
    23761 tcttatgttt ttaacttggt tggacttatt accgcagatg caagcttttg aacaccgttt
    23821 attcggaaaa agagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
    23881 tataaacggc ggaagcagaa gaaattcctt cggaaataag ctgaaattca caaaaatttg
    23941 aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
    24001 cttttttgat cagcatgatt ttattcagtt atcttcaaaa tgatttttcc gatgtttttg
    24061 tttgcttcca tatattcatg cgcttgtttg actgcgctga attggaatgt acggtcaatg
    24121 attggccgaa tcttgccatt ttctagaaaa ggcccagctt tttgcataaa agtttgtgcg
    24181 agctctgctt tatactgatc acttctaggt gtcaataatg taccaagcag ttggacacgt
    24241 ttcccaatca atacaccaag gtctatctca ggcacgatgg tcccacctag cataccaatc
    24301 aagacccaac gtccatcaac ggctatagaa gctaaattct tttcccagta agaagcgccg
    24361 atgaaatcca aaataacatc gactccccga ccttcagtga tttttcgtac ctcttcttga
    24421 aagtcttgtt ttttatagtt gatcaattca tctgcaccaa gttctttaca aaaggcgagt
    24481 ttttcttctg atcctgcagt aacgatgatt ttcgcgtgag aaagctgtct agctaattgg
    24541 atcgctgctg ttcccacacc acttgctcct gcatggatca agacagtttc gccttcttgt
    24601 aattttccaa gccagtaaag tgtttgataa gcagtcaaaa aaacttctga gattccagca
    24661 gcagaaacat agtctagcgt atcagggaga aggattgcac gatcggcagg catcacagca
    24721 tattcggcat aaccacctag attgactaat ccgtatactt ttgtcccagg agtaaaagtg
    24781 gagatatcac ttttattctc aactacctct ccagctactt cgactcccaa aatagggtag
    24841 ggaggtttca aagagactga ttctcttttc ataatatctg ttcgattgac agctgctgta
    24901 tggactttga tcagtaattc gccttgtttt ggtttaggaa caggcacttc aatttgttct
    24961 aatacatcta cagaaccagg atgcttgatt gaaatggcac gcatggatct attcctcctt
    25021 tgcccattct tggaatgctg tattcaactc tttgctacct tcagaaagta ctagagtcgt
    25081 acgagaaata tccgtttctt ccactagagc ggcaagtaca gcagcaactg tagagcgagt
    25141 gatcgttttt gatgccttct cattaggctt agttaattga atggaagtaa cttcttcatc
    25201 gttagtcaat gtgactggtt gaagaatgac aaaatctaag ttagttcgtt tcagccattc
    25261 atctgcataa tgtttagtaa tataataatc tgtcatggac tcaggccatt ttgctggttc
    25321 atctgcataa acagcactga ccatcagata gcgagagaca tgcgcatttt cagctgcgat
    25381 cactgttttg attgcgccat ccaagtctac ttgcagaaga ttctttcctt gagagccggc
    25441 agcaaaaatc aatacatcga tgcctttgaa tgcttcggtc attttttcca cggaccaagt
    25501 taaatcaaaa gagacatacg aaacattctc tttttttact aatgtttggg aagcaacatc
    25561 tctcacccct gcaaaaattt catgggaact tggagctaaa tcttttatca ggtgtcggcc
    25621 aatttgtccg tttgcaccta caacaaaaat tttcatttgg atcatccttt cgacgtcaaa
    25681 tctttccatt caatagtaac accaaaagag aaaaaaatca gagatcggct acgatgacta
    25741 tcagttagta agagaagggt gcttgaagta tcttaacaag acattgcata ctgctgcagt
    25801 cgcaagacct gcgacgattc cttcaggtat accacttgta aaaataaccg ctagaatagc
    25861 tgaataaaca gcatttaaat ttgttccgat gactttggca taatcttgct ggaataaaaa
    25921 gtagatcata ttcatcacta aaatcgtatt ggtcaatgaa ccggaaagtc cagctaagaa
    25981 taaagaaacg gaatctgctt tttgtttcat tagtttgcgc agacctttat aaacgaaata
    26041 aggaaccacc ccaatcaaga tgcgaggaat aaacgcgatc agcaaggctt tccagcttcc
    26101 atgttctgtt ccgatgactg ggataaacgg agaaaagaca aaagacaaag gtgtttggat
    26161 aactgtgcta cggatcatgc tgatcagtcc aaatgtaccg ccaagaaaag cacctagtct
    26221 tggtcctaaa acgatcgaag caatgattac aggaatatgc atggttgttg cattgattgg
    26281 accgataggg ataaaaccta aaggcgtaac agctaataaa atcaaaatag ctaaaaacat
    26341 cgcagttaat gtaaagtttt ttgtattttt cataagtact ccttttttct aagcatttct
    26401 ttcattaaga gtcagcatga cctgttcgat gatcgtctga atatcagcta aggcaccttt
    26461 tccaaaatct ccacaagcta aaagagattc tcttggttca atttcctgat agccgacaga
    26521 tctcaatgtt tccagattac gctgagtgat cgggttttga tacatattcg tgttcatggc
    26581 aggggcaatc aattttggtg ttcccactgg aagcgccatt gcgactgttg aaagaagatc
    26641 gtctgcaatg ccgtttgcta gcttgccgat aatgtttgct gtagcaggcg cgatgagaaa
    26701 caagtcagct tgttttgcta gttcgatatg attgattacc tgcggatttg attcttgcat
    26761 gacgtctgta tggactgcgc gcttcgacag agattgcaag gttaatggcg tgataaaatg
    26821 tgtgctgctt ttagacataa tcacatctac ctgggcacct aatttgacga attgacttgt
    26881 aagatcagcg cttttgtaag cagaaatact tccagtaacg cctagtaaga tctttttcat
    26941 tttgattcct ccagtctaat tttctcagta atcaatgcag caatctcaga tttactttga
    27001 gcttcttcca ctgttccatc tttagataag agatagccgt ggtgtgtctc gccatggatt
    27061 tccgtcaaat catttgcgaa aataaaatca gttttgttat ttatcagact attttttgcg
    27121 actgccagaa gagattcctt tggtacatct actaatagtt tgaaaccaat gatttttgct
    27181 tttggctgcc attcacgtag ataagaaatg atttttggtg ttttcttcaa aacgagcaca
    27241 agatgctcgg tgttagaaga gatctttgaa acagtggtat ctgtctgttc attttccgca
    27301 aaccaattgt ccagttcttc ttgcgttgaa gaagtgggta ggttttttgc cagctgttct
    27361 tctgaaaaac taaaagcagg agtaaaatca ctgactgcca tactgtggat aatcgcatcg
    27421 tagtgttctt ttttcgttag ttcttctaat tgtaaaaata agtccagtgc agattcgatc
    27481 gtataaatcg ttatattgga gtgtcttttc ggtttcagtg cttgtttcgt agtcacgtaa
    27541 tcgattgtgg tatccggatt ggcagctaaa taatcagcta atgcttgccc taatcgtcct
    27601 gtcgaatgat ttgtaatagc acgtacttga tcgatttttt cagtcgttcc tccagctgtg
    27661 attagaatct tcatttatgg tcacctacat ttactagtgt tcttccttgg tgtgttccag
    27721 caagtagttt agatgctgtt tcaggcaatt gatccagagt aatctcttgg acgaccagtt
    27781 catctgcaat ctgcaaatcc gccatctgtt gccataaaga aagacgttga tctattggta
    27841 cattgacaga atcaattcca atcaactgga tgtttcgcag aataaaaggc aagacagtcg
    27901 cgtttagttt gattccccca gcattgccac ataaagctac tgcaccgtta taagagatca
    27961 atggaagaag actgcttagc tgctcgccac ctactgtgtc gatgacatag tcgatttgct
    28021 gttttcctaa agcttttgtt gtctctggca gaaattcatc aggtcgtacg acttgcaaag
    28081 cacctagact ttttagccat gtgacagact cttcttttct ggaaaaggca atgattgact
    28141 ggaatcctag tttatgtaac agggctatcg ctgtactgcc tacaccgcca gaagcaccag
    28201 taacgacaac ttttttatcg gaggtcatac cttgacggat taaagcgttc acacacaaca
    28261 tagcagtaaa ccctgctgta ccaaggatca tcgcttgacg cagcgtcata ttttttggta
    28321 gaggaactaa ccagtctcct ggtacttttt gatattggct ataaccgcct ggatgggtga
    28381 cgccaagccc aaaaccagta gcaagtacaa gatcttcttt tgaaaaacgt ggattgttac
    28441 tttggacgac agttccggat aaatcaatcc ctggagtcat cggatatgtt cgtatcacct
    28501 gaccagactc gctcattgcc aatgcatctt tataattgac atcagaatag gcaattttta
    28561 ccaagacttc attttcagcc aactcagtaa atggcagatc actgattgct gtatggaaaa
    28621 caggttcctt tattacctga aaagatcgaa aagcgttcat tcgtgagaca ctccttttaa
    28681 aattttcttt tatcatacca taaagcctac tcttttattc ttgttcttcc gtaaatttag
    28741 acaaaaatta ggtgaaaaat aaagaatcaa acaactagaa tttattccaa aagataaaga
    28801 aatattcagc aaaaaaaatt ttacaaaggg ttgccttttt caataaattt cgtatactaa
    28861 tacgagtggt agtaaacagg aagtttacta ttagaaaact tcgattcgga ggaaattatg
    28921 gaaattggtg agaagttaag aaatttgcgc atccaaaaga atttgacaca agaagaacta
    28981 ggcgaacgca cagatttgtc aaaagggtat atttcccagt tggaacgaga tttaagttca
    29041 ccatcaatgg aaacattctt ttctattctt gaagtcttag gtgtgactcc agaagaattt
    29101 tttcatcaag aagcagcgaa tctgcaagtg gtttattcaa aagaagatca tacggtttat
    29161 ttcgatgaag aaaacgggta tgaattggaa tggctcgtgg ctgattcgaa tgaaaaagaa
    29221 atggagcctg tcttgctgac ttttgaaggt gcaggagaat ataagacgtt tgaaccttca
    29281 ttatctgaaa cgtttatttt tgtattagaa ggggaattag agttaagctt aggagagacg
    29341 gtctatgtgg caaaagcagg acagtcgatc tattatcaag cgacaaaaca acatcaatta
    29401 aggaatcatc tcaaaaaacg aacaaaagta ttgattgttg caacgcaatc atatttgtaa
    29461 tatagggggt aaaaacgtgg ggaaaccaat catttcattt gatcaagtag tgaaacgcta
    29521 tgatgatgaa acagtattaa aaaaagtaag ttttgagatc gaacaaggaa aattttatac
    29581 actgcttggt ccttccggct gtggtaaaac aacgatccta cgtattatag ctggattcac
    29641 cgaagctaca gaaggggaca tctattttga agggaaacgg ttgaatgatt tgccagctaa
    29701 taagcgacaa gtaaatacag tcttccaaga ttatgcactt tttcctcata tgaatgtttt
    29761 tgataacgtt gcttttggat tgaagatcaa gaaaatgtcc aaatcagata tcgaaaagaa
    29821 ggtaaaagat gctttgcgta tggtccagct tcctggatac gaaaaacggg agattagtga
    29881 aatgtctggt ggtcaaagac agcgtgtagc gattgcacga gcaattgtaa atgaaccgaa
    29941 agtattgcta ttagatgaac cgttatcggc gcttgatttg aaactacgta cagatatgca
    30001 gtatgaactg cgggatctgc aacaacgatt aggaatcacg tttatttttg ttactcatga
    30061 ccaagaagaa gcgttggcaa tgagtgatga gatttttgtc atgaataaag ggaaaatcgt
    30121 ccaaagtggt acacccgtag atatttatga tgagccaatc aatcatttcg ttgctgattt
    30181 tgttggggag agcaatatcg tggacggtgt aatgatcgaa gataatctag ttgagtttgt
    30241 tggtaagcaa tttgaatgtg tcgatggcgg gatgcgtcta aatgaaccag ttgaagttgt
    30301 tttacgccca gaggatttga cgatcacgac accagataaa ggaaaactag tcgtaacggt
    30361 cgatactcag ctgtttcgtg gtgtccatta tgaaatcatc tgttatgatg aacagcaaaa
    30421 tgaatggatg gtccattcaa cgaaaaaagc taaagaagga agcaaagtag gattggcctt
    30481 tgagccagaa gatatccatg tcatgcgttt caacgagtct gaagaagaat tcgatgcacg
    30541 tcttgacagc tacgaagaat aggggggaca agaatgaaaa caatgcgcag gatctattcc
    30601 gtcccttata ttttgtggct cggtttattc gtgatcgcgc ctgtattaat gatcatttat
    30661 caatcttttt ttgatatgaa tgggcaattc acgctgaata attatctatc ttattttacg
    30721 tcggggacat cgctttctat gacgatcaat tctgtatggt atgcattttt gatcacgttt
    30781 ttcacgctga tcatcagtta cccgactgct tattttttaa caaaactgaa gcacaagcaa
    30841 ttatggctga tgctgattat tttgcctaca tgggtgaatc tgctgcttaa agcttacgca
    30901 tttattggga ttttcagtgt gcacggcagc gtgaatcatt tctttgattt tatcgggatc
    30961 gggccgaaac agatattatt cacagacttc agttttctgt tcgtggcaac gtatatcgag
    31021 attccattta tgattatgcc aattttcaat gcattggaag agatgaatcc ttctttgatc
    31081 agtgctagta gagatttagg cgcgaatagc tttgagacct tccgacgagt gatttttccg
    31141 ttattattaa atggtgtgaa aagtggtgtc caagcagtat ttattccttc tctttcttta
    31201 ttcatgctga ctcgtctgat tggcggaaat cgggtgatca ctttaggaac ggctatcgaa
    31261 cagcatttct tggttacaca aaactggggg atgggttcta caatcggtgt gattttaatc
    31321 attgctatgt tcatcgtgat gctcctgaca ggcgagaaga aaaaaggagg tcgctcataa
    31381 atgaaaaaat ttaaatggtc gtatctttat ttagggatcg tctttttatt cttatatgca
    31441 ccgatcttct atctgatttt ttattccttc aatgaaggag gaacgatgaa cagctttaca
    31501 ggatttactt gggaaaatta tgaagctgtt tttgaagata cacgtttgat cattatcgta
    31561 ttgaatacgt tcatgctggc tttcttatct gcattgatcg ccacgatcat cgggacattt
    31621 ggggctatgg gaatctatta cacgaaaaaa cgacgtgcac ggacagcgtt gttgagcatg
    31681 aataacatct tattagtctc tcctgatgtt attatcggtg ctagtttctt gattttcttt
    31741 acactactag gattcggatt aggttttcag agtgtattgc tttcccatat tgctttcagc
    31801 attccaatcg tcgtcttgat ggtcttgcca aaattgcagg aaatgaacga ttcaatggtc
    31861 gatgcggcta gagaccttgg tgctaataat ttccaagtag tcaaaaatat cattttgcct
    31921 tatctttctc caggaatcat agctggatat tttatggcat ttacctattc tcttgatgat
    31981 tttgcggtta catttttcgt cacaggaaat ggctttacga cgctctcagt agagatctat
    32041 tctcgtgcaa gacaaggaat cagtttagaa atcaatgcgc taagtgcgtt agttttcttg
    32101 ttctccatga tattggtcat tggatactac tttattagta aagaaaaccg tccaaaacgg
    32161 ctgaagaaaa acagaagagt acgcgagggg gtggcacggc tgcgatgaaa aaattacaat
    32221 ctctgttttt aggtattctc atcattattg cggtgctgct gcttggtgtt cgtcaacttg
    32281 aaaaaacaag tggcatgagt ggagcaaagg tacttaccat ctataactgg ggagattata
    32341 tcgatcctag tttgatcacg aaatttgaaa aagaatatgg atacaaagtg aattatgaaa
    32401 catttgattc taatgaagcg atgttcacaa aaatccagca aggtgggaca aactacgaca
    32461 tcgctatacc aagtgaatat atgatccaaa aaatgatcaa agaaaaacta gtacttccat
    32521 tagatcattc gaagatcaaa ggattaaata atattgatcc acgattttta gacttggatt
    32581 ttgatccaga taatcagtat tcgattccat atttttgggg aactttaggg atcgtatata
    32641 acgataaatt ctttgatgca aatcaaatta agcattggga tgatttgtgg aagccggaat
    32701 taaaagacag tttgatgctg attgacggtg cacgagaagt tatggggctt tctttgaata
    32761 gtttaggcta ttctttgaat agtaagaata tggcacagtt gacagaagca gcaaataaat
    32821 tgaatcattt aacaccaaat gtcaaagcaa tcgtggcaga tgagatcaaa atgtatatga
    32881 tcaatgaaga agcctccgtg gcagtgacat tttctggcga agctgccgat atgatgtggg
    32941 aaaatgaaca tttacattat gtgatcccac cagaaggatc caatctttgg tttgataata
    33001 tcgttatgcc aaaaaccgtg aaaaataaag aaggtgccta tgatttcatc aactttatgc
    33061 tagaaccgga aaatgcggca caaaatgcag agtatattgg ttattctaca ccaaatgaag
    33121 aagcaaaagc gttattgcca aaagacattt cgtcggacga acaattctat ccatctgatg
    33181 acacgatctc ccatcttgaa gtctacaaag atctaggatc gaaataccta ggcatctata
    33241 atgacctatt cttagaattc aagatgtata ggagataatg gcaaaactca gtaagaatgg
    33301 ggacttatca acgttctaaa gagctaaaaa tcgtcctttt tctatctgag ttttccaatc
    33361 ttctaccaaa agcggatgaa acgccgcttt ttttgtgtta ttttattttt tctaccacgt
    33421 ttttctacca gagaaaagtg gacaaaacca ttgtaaaata gggcccgaga cgggctagta
    33481 aatctaagat tgaaaaagtt acagaaggat aaagtaattg gaaactatgc aa
//
LOCUS       DAZUKZ010000022        32178 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000022 DAZUKZ010000000
VERSION     DAZUKZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.022"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(407..766)
                     /locus_tag="ACG8EF_000977"
     CDS             complement(407..766)
                     /locus_tag="ACG8EF_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFS4946321.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="ACG8EF_000978"
     CDS             complement(796..1137)
                     /locus_tag="ACG8EF_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946322.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="ACG8EF_000979"
     CDS             complement(1134..1808)
                     /locus_tag="ACG8EF_000979"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4946323.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ACG8EF_000980"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ACG8EF_000980"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFS4946324.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="ACG8EF_000981"
     CDS             complement(4322..5512)
                     /locus_tag="ACG8EF_000981"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4946325.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="ACG8EF_000982"
     CDS             complement(5533..6060)
                     /locus_tag="ACG8EF_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFS4946326.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="ACG8EF_000983"
     CDS             complement(6107..8896)
                     /locus_tag="ACG8EF_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFS4946327.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="ACG8EF_000984"
     CDS             complement(9043..9243)
                     /locus_tag="ACG8EF_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4946328.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="ACG8EF_000985"
     CDS             complement(9696..10016)
                     /locus_tag="ACG8EF_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946329.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="ACG8EF_000986"
     CDS             complement(10519..11637)
                     /locus_tag="ACG8EF_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946330.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            11870..11992
                     /locus_tag="ACG8EF_000987"
     CDS             11870..11992
                     /locus_tag="ACG8EF_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946331.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            12108..13415
                     /locus_tag="ACG8EF_000988"
     CDS             12108..13415
                     /locus_tag="ACG8EF_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFS4946332.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="ACG8EF_000989"
     CDS             13536..14735
                     /locus_tag="ACG8EF_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS4946333.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="ACG8EF_000990"
     CDS             complement(14762..16030)
                     /locus_tag="ACG8EF_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFS4946334.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(16086..16152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(16200..16841)
                     /locus_tag="ACG8EF_000991"
     CDS             complement(16200..16841)
                     /locus_tag="ACG8EF_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFS4946335.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="ACG8EF_000992"
     CDS             complement(17250..17753)
                     /locus_tag="ACG8EF_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4946336.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="ACG8EF_000993"
     CDS             complement(17876..18640)
                     /locus_tag="ACG8EF_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFS4946337.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="ACG8EF_000994"
     CDS             18748..19023
                     /locus_tag="ACG8EF_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFS4946338.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="ACG8EF_000995"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="ACG8EF_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFS4946339.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="ACG8EF_000996"
     CDS             complement(20579..20980)
                     /locus_tag="ACG8EF_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4946340.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="ACG8EF_000997"
     CDS             complement(20994..21803)
                     /locus_tag="ACG8EF_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4946341.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="ACG8EF_000998"
     CDS             complement(21790..22680)
                     /locus_tag="ACG8EF_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4946342.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="ACG8EF_000999"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="ACG8EF_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="ACG8EF_001000"
     CDS             complement(23348..24289)
                     /locus_tag="ACG8EF_001000"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFS4946343.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="ACG8EF_001001"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="ACG8EF_001001"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS4946344.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="ACG8EF_001002"
     CDS             complement(25274..26470)
                     /locus_tag="ACG8EF_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4946345.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="ACG8EF_001003"
     CDS             complement(26488..27216)
                     /locus_tag="ACG8EF_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4946346.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="ACG8EF_001004"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="ACG8EF_001004"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS4946347.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="ACG8EF_001005"
     CDS             complement(28628..29086)
                     /locus_tag="ACG8EF_001005"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS4946348.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="ACG8EF_001006"
     CDS             complement(29083..30546)
                     /locus_tag="ACG8EF_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS4946349.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="ACG8EF_001007"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="ACG8EF_001007"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS4946350.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32178
                     /locus_tag="ACG8EF_001008"
     CDS             31602..>32178
                     /locus_tag="ACG8EF_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4946351.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         9385 a         6921 c         5511 g        10361 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtcttta
    32161 tttttcaatt ttttaaat
//
LOCUS       DAZUKZ010000023        31353 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000023 DAZUKZ010000000
VERSION     DAZUKZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.023"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(69..1172)
                     /locus_tag="ACG8EF_001009"
     CDS             complement(69..1172)
                     /locus_tag="ACG8EF_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFS4946352.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1801..2271)
                     /locus_tag="ACG8EF_001010"
     CDS             complement(1801..2271)
                     /locus_tag="ACG8EF_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFS4946353.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            2601..4241
                     /locus_tag="ACG8EF_001011"
     CDS             2601..4241
                     /locus_tag="ACG8EF_001011"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFS4946354.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            4395..5717
                     /locus_tag="ACG8EF_001012"
     CDS             4395..5717
                     /locus_tag="ACG8EF_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFS4946355.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(5785..6219)
                     /locus_tag="ACG8EF_001013"
     CDS             complement(5785..6219)
                     /locus_tag="ACG8EF_001013"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4946356.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(6284..7121)
                     /locus_tag="ACG8EF_001014"
                     /pseudo
     CDS             complement(6284..7121)
                     /locus_tag="ACG8EF_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(7690..7995)
                     /locus_tag="ACG8EF_001015"
     CDS             complement(7690..7995)
                     /locus_tag="ACG8EF_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFS4946357.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(8020..8451)
                     /gene="ruvX"
                     /locus_tag="ACG8EF_001016"
     CDS             complement(8020..8451)
                     /gene="ruvX"
                     /locus_tag="ACG8EF_001016"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFS4946358.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(8448..8714)
                     /locus_tag="ACG8EF_001017"
     CDS             complement(8448..8714)
                     /locus_tag="ACG8EF_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFS4946359.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(8969..9694)
                     /locus_tag="ACG8EF_001018"
     CDS             complement(8969..9694)
                     /locus_tag="ACG8EF_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS4946360.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(10055..10125)
                     /locus_tag="ACG8EF_001019"
     tRNA            complement(10055..10125)
                     /locus_tag="ACG8EF_001019"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10091..10093),aa:Gly,seq:ccc)
     gene            10242..10994
                     /locus_tag="ACG8EF_001020"
     CDS             10242..10994
                     /locus_tag="ACG8EF_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS4946361.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            11010..11489
                     /locus_tag="ACG8EF_001021"
     CDS             11010..11489
                     /locus_tag="ACG8EF_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS4946362.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(11740..13320)
                     /locus_tag="ACG8EF_001022"
     CDS             complement(11740..13320)
                     /locus_tag="ACG8EF_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFS4946363.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(13313..14056)
                     /locus_tag="ACG8EF_001023"
     CDS             complement(13313..14056)
                     /locus_tag="ACG8EF_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4946364.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(14232..15008)
                     /locus_tag="ACG8EF_001024"
     CDS             complement(14232..15008)
                     /locus_tag="ACG8EF_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4946365.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(15256..16092)
                     /locus_tag="ACG8EF_001025"
     CDS             complement(15256..16092)
                     /locus_tag="ACG8EF_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFS4946366.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(16103..17032)
                     /locus_tag="ACG8EF_001026"
     CDS             complement(16103..17032)
                     /locus_tag="ACG8EF_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFS4946367.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            17224..17577
                     /locus_tag="ACG8EF_001027"
     CDS             17224..17577
                     /locus_tag="ACG8EF_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS4946368.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(17651..18307)
                     /locus_tag="ACG8EF_001028"
     CDS             complement(17651..18307)
                     /locus_tag="ACG8EF_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS4946369.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            18533..20467
                     /locus_tag="ACG8EF_001029"
     CDS             18533..20467
                     /locus_tag="ACG8EF_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4946370.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(20553..21776)
                     /locus_tag="ACG8EF_001030"
     CDS             complement(20553..21776)
                     /locus_tag="ACG8EF_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4946371.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(21770..23641)
                     /locus_tag="ACG8EF_001031"
     CDS             complement(21770..23641)
                     /locus_tag="ACG8EF_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFS4946372.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(23662..24780)
                     /locus_tag="ACG8EF_001032"
     CDS             complement(23662..24780)
                     /locus_tag="ACG8EF_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4946373.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(24863..25696)
                     /locus_tag="ACG8EF_001033"
     CDS             complement(24863..25696)
                     /locus_tag="ACG8EF_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4946374.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(25689..26606)
                     /locus_tag="ACG8EF_001034"
     CDS             complement(25689..26606)
                     /locus_tag="ACG8EF_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4946375.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(26833..27957)
                     /locus_tag="ACG8EF_001035"
     CDS             complement(26833..27957)
                     /locus_tag="ACG8EF_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4946376.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            28140..29048
                     /locus_tag="ACG8EF_001036"
     CDS             28140..29048
                     /locus_tag="ACG8EF_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4946377.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            29189..30802
                     /locus_tag="ACG8EF_001037"
     CDS             29189..30802
                     /locus_tag="ACG8EF_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4946378.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         9659 a         6190 c         5342 g        10162 t
ORIGIN      
        1 tccttgaact ataaaaatag gagtacggca tcatctgccg cactcctatt tttttcgtca
       61 tttatttctt aaataaaacg attctcacgc aattttattt ttttgatatc ttctactgtt
      121 tgtgttccag ctaattgcat gaccatttcc aattctgttt taaagaaatc aaacacttga
      181 tggactcctg tacttcctcc taaagacaat ccataaataa cgggtctgcc aatagcaaca
      241 agatcggcac ctgatgcgat tgctttgaaa acatgttgtc cacgtcgtac accgctgtca
      301 aacacgattg gtacacgttt atctactgct tctgccacat attgcaacga atcaaacgca
      361 gcaggccccc catctaattg acggccacca tggttagata cccaaattcc accagcacca
      421 gattctaatg atcgatagac atcttcttct gattgaacac ctttcacgta gactggcaaa
      481 tcagaatgag cagcgataaa ttctacatct tttggactaa gtttttgttt ggaagattta
      541 tatacagcat ccatcgtttg gcctactcct gattgataag cctgaacgat cggcattggc
      601 agcgggaagg taaacccgtt tctgcggtct gtttcacggt ttccgccaac tgttgcatcc
      661 gctgtcaata cgattgcttt tgctccatta cgcttggcca cttcaagaat atccaaattg
      721 attccattat ctttgctcat ataaaattga aaccattgcg gtgctttctc cccgcctgct
      781 tctcttatct cttctaaagt acaagaagcg taagaacttg ctgtatagat cgtcccaaaa
      841 tctgctacac cttttgcaga agctttctcc gccttcacat gagctaatcc atgagcagca
      901 actggtgcca tgatgattgg tgcagtcagc atttcttcgt caaaatgtgt agttgtatcg
      961 ggtaattcta catctcttaa tacatgagga ataatcaaac gatgattgaa tgcccgttca
     1021 ttttcttgat aagtaaataa gtcgccagca ccactgctga tgtaaccata accaccagct
     1081 ggaatcactt gtgaagccgc gatttctaaa tcttccatat tgataaaatc tactattcct
     1141 tcatttgtac ctgcttgata tgttttttcc atctcaatgc cttctctcta taaaatttga
     1201 atgatgaaca atatgatagc gtttacactt tttatattac tcttcgacat tgatacaggc
     1261 aaataatctg tttacatttc agccattcca gatgtcacta ctctggaaga aatgattgta
     1321 ataaaaatag ataaaaaacc gatgggactt tgacaaaagt agtgtcaaag tccctttttt
     1381 tgaataaacg gtgttcaaaa gccattttct cggtattaag tcaaatttat gcaaaaatgt
     1441 ggaaagccat tttcacaaaa tttttcttac tactcgaaac taagcgactt tttcacaacc
     1501 tctcgataaa acggtgttca atcagctgac cctcggtatt agctcaaaaa atggcaaaat
     1561 atgaggaact attttgccat ttgtctctct aatactcagg tcagaacgtt gacttcacag
     1621 tctcttcctt aaacggtgtt caaaaacttg catctgcggt aataagccca accaaacgaa
     1681 aaataggagg agctattttt gtttggctgt tcttattact tgatgcaggg caggtttttc
     1741 acgacctctg attcacggtg tttctttttc atcaacattt ttacgaaata ctgtggaaga
     1801 ctattgaaat aagtgtggat tgtttctttg tgtttcaatc gtttcttgcc attcttcatt
     1861 taattttgga ttaccgtttt cttccttgaa ctcataaggt cctaattcaa aaatcccaga
     1921 cagtatttga ttcacgattt catatagttc agtgtgatca gacacatgat tcttgaagat
     1981 catctcatta tcaccacgtt tgaacctcac attcgggctc tcatgatgaa cctctatgca
     2041 tttttctcct ttttttaact gacggacttc tgaaaacatc tcataaatat cttctccagt
     2101 aaaaccttca gcaagcgcca gactgtccac aagaccgcct actgtcagtt cagagattat
     2161 tgcttgattg gaatctaatt ttaatgtata gcttgtcgct tctactacgc cacatgcatt
     2221 gcaaccgcct tctgatagtt cgctttttct aatcaattga acttctttca ttttctcttc
     2281 ctcctcttaa ttttctactt atgtattttt atcacctgac ataggtgact cctatcaacg
     2341 ctatcatgtg cttttttcct cagaaatcct atttatccat tccatctatc ccttattctt
     2401 ctacttttct ttctctagca tataagagat acgcatagta ttcaagcaag gattgtcgat
     2461 ataaaaagaa caaatactgc tattttgatt tcttttggct atattacata taaataaaat
     2521 ttatcatacc tgctcttttt gactatatca tcatctgact tctatacttc gtttgtatta
     2581 aaacttagga ggaatcattc atgcttacag ggttaaactt attaaataat ccttttctaa
     2641 ataaaggaac agcttttaca aaagaagaac gggcaaaata tggaatcact ggtatgcttc
     2701 caagtactgt ccaaacactg gaacaacaat ctgttcaagc ttatggtcaa tacttaagca
     2761 aacaaacaga tttggaaaaa cgtatcttct taatgaatct attcaatacg aatcggacat
     2821 tgttttataa attgatgggc gaacatttag ttgaattcat gcctgtcgtt tatgatcctg
     2881 tcgttgcgga ttcaatcgaa caatacaacg aaattttctt gaaaccgcaa gatgctgcat
     2941 ttctttcaat cgacgaacca gaaaacatca aagcaagttt gaagaacgcg gcagatggac
     3001 gagacattcg tttgattgtt gtgactgatg cagaaggaat cttaggtatg ggcgactggg
     3061 gtgtgaacgg tgttgacatc gctatcggca aattgatggt ctatactgct gctgctggta
     3121 ttaatccagc acaagttttg cctgtatcga tcgacgctgg tactaataat gaagaattat
     3181 tgaataatcc cttatactta ggcaatcgtc atgctcgtgt agaaggcgaa acttactatg
     3241 aattcatcga tcaattcgtt caatcagcta ccgaattatt cccagaattg ttattgcatt
     3301 gggaagattt tggacgagga aatgctgcag cgatcttaga aaaatacgaa gatcaaatca
     3361 ctactttcaa cgacgatatc caaggtacag gaattgttgt cctagcaggt gtattaggcg
     3421 gattgaatat atccggtgaa caattgaaag atcaaataat tttgacattt ggtgctggta
     3481 ctgctggtgt gggtatcgca aacatccttt tagatgagat gatccgtcaa ggtgtacctg
     3541 aagaagaagc acgccaacat ttctatcaag tagataaaca aggtttattg tttgaagata
     3601 cagaaggatt gactcctggt caaatcccat tcgctcgcaa acgctcagat ttcactaatg
     3661 ctgatgaatt gacaaactta gaagcagttg taaaagcgat ccatccgaca atcatgattg
     3721 gtacttctac ccaacctgga gcattcactg aagaaatcgt gaaagaaatg gctgctcata
     3781 caccacgtcc gatcatcatg cctttgtcta atccaacaaa attggcagaa gcaaaagcaa
     3841 aagatttaat agaatggacg gacggaaaag cattagttgg aacaggtatc cctgctgctg
     3901 atgttgaata taatggtgta acttaccaaa tcggtcaagc aaataatgct ttgatgtacc
     3961 caggtttagg acttggtttg attgcttcaa cagctacacg agtaaatgct gaaatcattt
     4021 ctcaagctag tcgagcatta ggcggaatcg ttgatgtaac aaaaccagga gcagctatct
     4081 tgcctccagt agcaaaaatc actgaatttt ctcaaacgat tgctgaaact gtcgcaaaat
     4141 cagttgtcgc acaaaacttg aatcgtgaag aaatcacgga tatcaaagaa gcagttgaat
     4201 ctgctaaatg ggttcctgaa tataaatctt tagaagatta agatcgttat agtaaaagaa
     4261 catacagaaa ctttgtatga cgtctaaaaa gaaaaaagcg actgcggcaa aacagcggcc
     4321 agccaactcc tgctcttttg tcgcttttct ttggcttttt tcatatgata cagaataaga
     4381 aaggatctgt aaaaatggca caaaaaacaa caactgcaac tgcagttgag gaaaagaaaa
     4441 aagaaggttt ttggtccaca aaaatcaccg gcgtaagttt gccactttat ttgatcatgg
     4501 tccttgtcct gatcattgca atggctttag ggaaattacc gacgaatatg attggaccaa
     4561 tcgcagtttt ggttattttc ggtaatttat ttcattttat cgggacaaaa atccctatcg
     4621 tcaaaagtta tttgggtggt ggttccgttt ttgcgatctt cgcttctgct gcgattgcta
     4681 catttgggat cttacctgaa tatgtagtga aatccacaga aaactttgtc agcaatatgg
     4741 gcttttggga cttttatatt gctgccttga ttactggtag catccttggt atgaaccgta
     4801 atctattgat gaaagcttct gttcgtttta ttcccgttgc acttatttca atggtcgtct
     4861 ccttttttgc cgtaggactc gtgggcatgc ttatcggaaa tggttttgca aattccgttt
     4921 tatacatctc attacctgcg atggctggtg gtgttggagc cggagccgtt cctttatcaa
     4981 cgatttatgc aaatgtttta ggtacggatg cttcttctat catttcccgc ctgattcctg
     5041 cgacagcaat gacaaatgtc ttagctatca tcggtgcagc attagttgct cgtgctggac
     5101 aatcaatgcc aaaattcaac ggcaatggta aattaatgga aaaaggcgat cttggcgatg
     5161 gaaaacctcg aactgtgaaa ccttccattc cacagttagg tgttggtttg atggtatcat
     5221 tgactttctt catcttagga caaattggta actattttgt tcctaaagtc catgcttacg
     5281 catttatgat tattatcgtt gtcatctgta aaatcgccaa cctattacca gagtattatg
     5341 aagatgcagc tatcatgttc aataacttga ttgtcaaaaa cttgacagca gctgttcttg
     5401 ccggtatcgg tattgcgttg ctgaatctga atgtattagc ttctgctttg acatggcaat
     5461 ttgttgtctt atgtctgaca agtgttatcg cgatttcagt tgtttcagga tttgtcggtc
     5521 gtttgtttgg tttatatcca attgaatcaa ctattactgc tggcttatgc aacaactcga
     5581 tgggcggaac aggaaatgta gccgtcttat ctgcagctaa tcggatggaa cttattgctt
     5641 ttgctcaaat gggcaatcga cttggtggtg ccatcgtttt gatcatctct ggtttcttga
     5701 tgcaattact ttcttagtca aaaaaacaaa aatacctgat catcaagtat cggcttggtg
     5761 atcgggtatt tttttgataa agatttacag gattttcaaa aatctttttt gcattttatc
     5821 ggaatgataa ccgatgtgtt cataaaattg gtgtgcttct actctgtatt ctgcagaatt
     5881 aagccttatt gtcgttattc ctcgtttttt cgcttcttct tctacgccag tcatcaaagc
     5941 ctttccgatg cctctttttt cagcatattt agacacagca agtgccataa tattgaacat
     6001 tggatcgcta aaaatcgatt cataggactc cgcttgtaca tagccagctg cttttttaga
     6061 agtaggatct tcataaacca agaaaaaatt tttctctggc tccttcagca gtttcttcat
     6121 ttgtctcacc gtaacggtta atggtatatc ataccctaat tgatccgcat tgatttcacg
     6181 tagtgcttct gcatcttcta aacgcatttc ccgtaacatc caaatcacca gccaatttct
     6241 gattttttaa aatgaatttt tttatcatag tatactcttt ttttcattta gaacaatatg
     6301 tttcagtgat tttgacttgc gttagtctag attctcctaa tgtttgtaag atgacaaagg
     6361 cattgtaaca attgaccaaa agaaccacga ggacaattac ttgaaaaata aaggtcactt
     6421 tttttggaga caaaaccctg ctaaatcttg ctccccacaa acctccaata acagcagcgg
     6481 ggataacata aaacaacata gataaatcaa agcgcataaa gctacttgtt aaagcaatcg
     6541 ttaccaattt tgccagttga gaaaagaaaa tcgtagatag agaatatagt gttgcttctt
     6601 ttattggcaa tgcaaacatc agcatcaaca aagaaacatt gactggtcca ccaccaatac
     6661 ctaaaaaact agctaaaaag cccagaaccc cccccacata taaggtacca agtccatgac
     6721 gtcaaatgaa attttggctg atgatgcttg gtgtagaaga acgcaaaaac caaggtcaat
     6781 accgtcaaaa aaatttggat cagctgcaca tgcttctcgt tttcaaataa ctgtaaaaaa
     6841 acttcgaata caatgttgcc gactactcct ccaacaacag ctccactaga tacccaaccg
     6901 acaatctgcc aattcagtga tttcccgctt gatacttgtc gcacagtcga aacaatggac
     6961 atcgtgaata ccgcaactgt tgagtaaaaa gaaatccctg caacagaatg ggcgccgata
     7021 agatcaagaa tcggtttgat caacacgcct ccacccatcc cggaaatagc gccaactgtg
     7081 ttcgccagaa cgatcaccac aaaataaatc agaccaatca tcttgtattt cctttctctt
     7141 gtttatatct tttagcgatt gttactcttt tcttttatta tacagtaaaa gaaacaaacg
     7201 aggcttcgaa agcttttcta ctttttaagg aaatttagtg ctaaacgaaa agaggttgtg
     7261 aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagctc ctcataattt
     7321 tcgtttggtt gaacttatta ccgcagatgc aagcttttga acaccgttta agaaagaggt
     7381 cgtgagcctg ccgttcagct ccgagtcaca aggaacaatt tcaaaaaaca gcttctcatg
     7441 tttgttgaat attgtgactt gtgaccgagg agttggcagg agaacaccgt ttaaggaaga
     7501 gactgtgaag tcaacgtttt gacctgatta ttagagaaac aaatggcaaa atagttcttc
     7561 atatttcgcc gtttttcgag cgaatgccga ggggcagctg attgaacatc gtttatttgg
     7621 aaaaagggat tgtgacaaga cgtttgtcac aatccctttt tttatatctt attaattcat
     7681 tcttttgttc tattcttctt cttccgccat gaatgtattg aatacttctt caatcatgtc
     7741 ccattctgcg tctgtttcga tttgttctaa ctcgccttct gttccatctt cgttttcgat
     7801 gtatgcataa gcttgtaatt ctacatcttc atcttctgaa acaccagcag gatataaaag
     7861 aacatagtta cgtccaaatt cttcttgtcc gtcaactgtt agtaggattt catataatgt
     7921 ttcatttcct tggtcatcca ccaatgtgat gtgttcgtgt ccttcatggt cgtgggaatg
     7981 gttatgttct gtcataagat tacctctttt cttttattat taaaataatt gcttacctga
     8041 gccgtctaaa taattttgta agatcatcac agctgctaat ttatcgatga ctttttttcg
     8101 tttggctctt gaagtatttg cctgctctac tagcatacgt tctgcctgaa cagtggttaa
     8161 tcgttcatct tgataatcaa ctggcaagga gaatagttct ttgatctttt ctccgtatga
     8221 catggaagct tctgcccttg gtccgatgga attgttcata tttttaggta atccaactac
     8281 aaaccggtct acttcatatt gtgctactaa ttccttcaac cgatcaaagc caaactcttc
     8341 ttctgcttca ttgatccgaa tgatctctac tccttgagct gtccagccaa aggggtcact
     8401 gatagcaaca cctactgtct tagaaccgac atccaagccc attactctca taaattcact
     8461 ccatgatttg agaggtagtc cttgacaatt acttccatga tctcatcgcg ttcgtgacgg
     8521 cgaatcaaat ttcgagcatc acgataacga ggaatgtagg ccgggtctcc agaaagcaag
     8581 tagccgacaa tctgattgat tggattgtag cctttttctt ccaaagcccg ataaacaatc
     8641 gctaatgttt cgctcacttc ttttttgcga ctatcgtcaa aaccaaaacg tacggtttca
     8701 tctgtaaaac ccatgattct acacctcttt cccgcaaggt aattccagct taccttctac
     8761 aaccttattt tactcgaaga aagggaaaac tacaaacaaa tattccaaac ttccgaataa
     8821 attaagaaac actatttgta tgtttttttc cttttttctg tagctttatc aaaagtactc
     8881 ttttcataaa gtatacgcta tatttctttg aatgtatgtt tttacacaat tttttcataa
     8941 tattcataaa aaattcgaat agctgctact aattttttgt ttttgttgct tgtcctgtct
     9001 cataattgat cgtgtagcca tcttgaacat tgtagataaa gacattgaat tcaatttttt
     9061 tgtcttcaat gctttctgct tccaattgca ctcccctggc taccagttca ttcccctcaa
     9121 aatgaggagt gacacggtag cgtacatgat gatttgtttc tttgatatat gccgcaatat
     9181 cattttcgtg agcaagcatg tatggagcat tcaacgaacg tgtccctgtc atcaagtttt
     9241 taatgttgtt attttgacca gtcagctgga aaccaattaa atggctgcga ttatataacc
     9301 attgcccgtc acttaatttc ttttgtttcc aacctgtcgg gtcaatatac aaaggctctc
     9361 tcttttcagt agggaacaaa tcttttccaa gcatagcatt ggcttgtcct actctgttca
     9421 atttatcaat atttgaatag ctttcccatg ttcctttgga aaggctaaga tcctcttttg
     9481 taaattccgg ctgattccca ttgatttcta tttcttgatg ttttccatca tattctggca
     9541 catccgctac ttgagaagct tgtggtgtgg aatcaaaaat agttgtaatc tgctgaaaga
     9601 tatctgaatc tttttgtgtg gtggaaacac cgaatgcccc tgcaatcaca gcagcgacga
     9661 tccccacgat cgccttggtt gtttttttgc tcatttcttt actcctttac taacagctcc
     9721 ctaaatctta acatatgttc ccctataaat aaatactaga aagaaataga aacagcaaaa
     9781 aataaaacct gttttaagat ggcttaaaaa tacaaaaaag agacggattc ctcgtctcta
     9841 atcagtcacc actctatctg taaggtattt attattattc aaaaattttc ttcatactca
     9901 agacaataca ctgatttcac aaccgcttgg taaacggtgc gcaaaagctt gcatctgcgg
     9961 taataagtcc atccaagtta aaaacataag aagttgtttt tgcttggctg ttcttattac
    10021 ttgttgcaga gcagcttttt cacaacctat tttaagcggg tgacgggaat cgaacccgcg
    10081 acatcagctt gggaagctgg gattttacca ctaaactaca cccgcaatac aaaaacagta
    10141 tacgcctctt ttattccttc ctcaagtttt tttattcttt acgcttctgg tatgttttgt
    10201 agtaaaataa aactaatcaa tcaattattg gagaagatgc tatgacggga aaagaattag
    10261 atctgttgct ggacacttgt ttactcgctg ggaaaatcat gatggaaagc aatgcggaga
    10321 tgtatcgggt agaagatacg atgagtcgaa ttgctttagc ttctggaaat ttccgtttgg
    10381 tcagctatgt cactcaaacc ggtctcttta ttggtcttga tcgtacctcg accatccgaa
    10441 tggaacaaat taccaaccgt tcgattaatt tagaaaaagt agtaaaagta aatgatcttt
    10501 cacgtaaata tgttgcttca gagctaacat tagaagaact ttactgcgaa ctgcaagcaa
    10561 tcgaccgaga ccgttctttc tttcctgtct ggttgcaaat cgttagcgcg gcagtgatca
    10621 gtggaacaat tatggtgtta tttggcggaa agatgaccga tttacctgtg actttagtca
    10681 ttggaggatt gggatacgca gcttatctat atagtttgaa attttttaga atcaaatttt
    10741 tatcggaatt tttatcttct ttattgattg gcatagccgc tatctacagc gtacgttttg
    10801 gcttaggaag caatcaggat ctaatcatta tcggatgtgt gatgccttta gttcctggag
    10861 ttcaaattac gaacgctatc cgtgatttgc tagctggaca ttatgtctca ggagtttctc
    10921 gcggaacaga agcgatgatg actgctacga tgatcggttt tgcgattgct tttattttcc
    10981 agctttttta ttaggaaagg aacacaagta tggggacgat atttattcag ttttctttta
    11041 gttttttagc gactgcggca tttgccgtta ttacaaatgt acctaggcgt tcattgattt
    11101 gttgtggtct ttctggtgct tttggctgga tgatctactg gatctgtgtt caaataggcg
    11161 gatctgctgc ttttggttca ttgcttggtt ctttaggagt agcattcatc agcgatcttt
    11221 tctctaaacg tttgaaaatg cccgtaacga ttttcaatat tcccgggatg gttcctctag
    11281 tccctggtgg tttagcttat caagcggttc gaaatctcgt tactgaatcc tatcgagaag
    11341 cggctcatta tgcggtccaa gcaattatga ttgctggagc aatcgcttta ggtcttgtat
    11401 tatcagaagt ctttaaccat aacatccgta atttcaaagt aaagcgtgac gcaatcatga
    11461 tgaaacgaaa aaaacggctt tttaaataaa cgaagtgtaa caaagaaaag gttgtgaaag
    11521 agacgttcag ccttgagcga tgacaaaata tctcacaaac attgcgacaa gtaaccgcag
    11581 gagcaataag aagtaattat gagtattaaa aaaagttttg taaaaatagc ttctcacatt
    11641 tttacataaa attgacttaa tactgagaaa ctgacttttg aacaccctct atctgtaaaa
    11701 aaggggctgc gacatgattt ttgtcacggc ccctcttctt tattcaaatt ttttccaaaa
    11761 cgttcgttga taataaacga tccatagagc ggaaagtatt agaactaaac cgatcacagg
    11821 aatattcact aaccaaaatg gataaaacaa agccaacatt ccgtataaga cgataagaat
    11881 aatactgatg atcaatgaac ccatcatagt tgcgactaaa cccaaacttc ctgaacctct
    11941 gttaaataat tggttaatat tggtccagtt cagcagcagc aaccgataat ctctagcgaa
    12001 ataacgtagt gacaataagt agcagccgaa taaagaacca aatagcaagc tagggataaa
    12061 aaacaaaggc agctgaaaag acagtccgct taataatgcg atcccaccag ttaaaaaact
    12121 ctgcaatatc catccgaatc tgaacttttc ttttaaatat tggttcatag agattggcaa
    12181 agaacggata aacagaaaat tttcttgatc caaagaaata agattgctga taaaagaagt
    12241 ctgattaact gttaagaaag ccaatgcgat ccctaccagg aaaacaacac cgattaactt
    12301 attatctaaa tcagttaatt ttatttcgcc tcctagggca aaagcaagaa taaaaatgat
    12361 tggagttaac aaggaagaag aaagaacttg cataatcaaa ttaggttctt ttactaattg
    12421 agcattgtaa ctaaataaaa gctgatgcac attttggttc tttttatggg ttctttttat
    12481 tttgaactca ctgtttgaat cagtcagttg ctctgtaagc tttggtacta tcagctgttt
    12541 tacagcaagg aaaaatccca gagccgtgat agctagtatt ccccaaccca ataaaccttg
    12601 ttgagaaagt ggctgactgg cagcatcata gaatggcaat aatatcgaaa tcggatgccg
    12661 atctgttaac tcattttcaa tagtccccgt ttgatgattc atccacatga ttccagcaaa
    12721 tgcgatcagc atcgagctgc caagcaagat actagaaact gtttttctat gttttttaaa
    12781 cactgctgtt cgagccaaac caaaaacaat caaactagcc agacaaaaaa gaataatcaa
    12841 aaatagagaa aacaaaagga ttgagataat gatagtaagc aaaatcccta gatcagctcg
    12901 ccatccggtt aaaataaaca tgactagaag cggaaaaaca aaaggaatga tggtcaaagc
    12961 aactaccaaa atctttgctg aaaaaacctg ttcttgtcca aaaggcagag gcaaaaatac
    13021 ttgcaggtca tttccttcaa aaaagatatt ataaatgact gatatacttt gagttaaacc
    13081 taacatacca aaaagtgcca cgtaataagt aaagaaacct ggcaatttaa caaagttcat
    13141 agcaaacata gcaaatccgt aaatcagtaa aaaaatcagc cctgaaaaaa gattttgaga
    13201 caaaagatag atagttaatc gctttccctt ttttcctttc tctcttgctt tattcgtgag
    13261 ctgtggatta acatataaaa gattaactcg aattaattct aataattgtc gcttatacat
    13321 acggtccatc tcctgtcgct tgccttccag ccattttcaa ataaatcttt tctagcgttt
    13381 catcaggtga gccattcaat aattcactaa ctgccccctg ataaatcaat tcacctttct
    13441 tcaaaatagc cagtttatcg cataactgtt gtgcggtatc caatacatgt gtagaaaaaa
    13501 tgactgtttt tccttttgcg gcatgagcct tcatcatttc tttcaaatca aatgctgctt
    13561 gaggatcaag tccttgcaaa ggttcatcca atacccaaat atctggatct ggcagcaacg
    13621 tgccgatcaa aatcgttttt tgacgcattc catgtgaaaa acttgcgatt gtttcatttt
    13681 gatgactata catatcaaaa agcgctgtca attctgctaa acgcttttct tttttttgtg
    13741 tgtctaattc ataggcagct gaaatcaagt cccaatattc tccagccgtc aattgtaaaa
    13801 aaatatctgg ggaatcaggc acataaccga ttttttgttt gatactcaat cgattttccg
    13861 tcaatttcat tccatctact aaaatcgttc ccgatgatgg ttcaataata ctaactaaac
    13921 ttttaatcgt agttgatttt ccggcaccat tatgtcctaa aaatccaaaa atttcccctt
    13981 gtcttatcga gagagacaag tcttttaatg cttgtttttt cccgtaactt ttacttacat
    14041 tgatgaattc aatcatttct gtactccttt tgtttgttat tactttttat tgtacccttc
    14101 gttattggtg gagtaaatgt caaaaagagt gcataacaaa aaaggataga aacgactagg
    14161 ctgctacttg ggttgttctc ttctacagga aaacactctt ctaacaggta agatatgagt
    14221 ctttgttttc tttaaaatga ttcattttct atcttttctt gcataaaatt ctttagttta
    14281 tcaatacttc tctttcccat ctctagttgt tctccatttg caaatgttac tatttcgttt
    14341 actctgctaa tagatgtaat gttttcagta ttaatgatat aagcttttaa atctttacat
    14401 aaatactcat attcgagtga ttttttatat tttttaattg tgccgtttac gagaatctct
    14461 tcatctttca atactaggcc tattttattt ttataacctg gcatgctcat tagataaagg
    14521 atttcatcgt aatggatata gtgatcgcta ttaccataac ggacagttgt ccaccttttt
    14581 ttcgtagcat gttgaacagg atagttttct ttttctttca ataagtaact ttggatgctt
    14641 tttttatttt tttcataagt gaattctttt atcaaataac cactaggcgt aatctttcga
    14701 ttaataacag ttattgcttt attcggatga gacgttagat aaacaatttt acacttttca
    14761 ttcttttctc taagcatttc gcctagttcg ataccgctat aataggcgtt caaatcgatg
    14821 tctaggaaaa aaatatctga ggaatagatt tcattatttt taatgaattc aacaaattgt
    14881 ataacgttca atccctgaac gatctcaaat ttccaaggac ttaataaaat tctggattct
    14941 tccaaaattt ctactagcat tttttcaaaa accaaactat catccaaaat aaatatacgc
    15001 gttaccataa aaagtacctc cactcgatat tttactatca aaatcatttc atacaacttt
    15061 tcaacataca tgttaggaat aaattgtaat tctcttcaca atcttttcca acacctttga
    15121 aaccgatatc aaatactttc aggatgctgc ttctaaaata aatatcaaac aaatgaatac
    15181 atcatgcaaa aaaagccaga caaccgacgt ccttgcgtgt cagctgtctg agctatgttt
    15241 atttattttg ttcttttaaa aagaacgata cacatactga tttttcggag cagagacttt
    15301 ctcagcgttt gtaacaacta cagatgggat ttcacgcttg aatggttgat aatagtctga
    15361 ttggatggtt ccttctattt tataccattc atcattttta accgttactc cttccggcat
    15421 atgcgtcaac aaaccaaaga ctcctgaatc tgccacacaa tggataatgc cgaatcggaa
    15481 caagaagata tctgtattgc tatcgttcgg tgtttggtaa acaaatcctt catagctgat
    15541 ttttttgcca atgaattcgc tagggtaatt ataaattagc tccataactt ctaaatagtt
    15601 ttcatctgtg atcttgatag tttgcttatc cccatacttt tctttcaact cattcatttg
    15661 gtctaagtag tctgatttgt tgaaataaat actggtatcc ggctttaagt attgtgtctg
    15721 catctccgga tcccctactg attctttact tactgggaaa ttaaatcctt tggcttctac
    15781 gatcgtagtg tcgagactaa ctgttggaaa caacgtcccc acgattactg gcaaagctag
    15841 cagtacataa gcgattccac gttgataagg ttttgacaac ccatggtcat gatcgtgctc
    15901 gtgtgccttt tcttctgctt ttccgttttt gacccacaaa tacagctgaa ctaaagccaa
    15961 aagagcagat aaaatcattg aaagaatagc taagtagcga taatggacat tgatatattg
    16021 atcgagtttg ccagaaactt gcaagtacat catcaattcg aaatacccag ataaaatcaa
    16081 aaaccgcagc atattttccc tcctaaataa ataatgtgaa tccaataacg ctaagggcaa
    16141 tcagtccgat cagcgataaa ataaagcgtc ctgtaaaata tcgtttcatc atcagtagat
    16201 ttttgatatc gaccattggg ccaaagacca aaaaggcagc tacaggtgct gtgccgaaca
    16261 gacttaacaa agaagatcct ataaacgcat ctgcttctga gcaaagagac atcgtaaagg
    16321 ccaataacag catgatgata atggctaaaa tctttgttga gccgagcgtt agcataattc
    16381 tagttggtaa ataagtctgc atcgcagctg cgattaatcc gccaatgatc aaataacgac
    16441 ctgtatcaaa aaattcgtcg attgaatgtg tcaacacatg cccagtttga tggatcagct
    16501 gattttttgg ttcattcttc tcactttgtt cttcttcatg gacatgatga tggtgatttt
    16561 ctgctacttt agacgtgatt ttttctttca atacagcgtc tttttgaaaa taggcaagcc
    16621 agacaccaat cacaagggca acaaaaaagc tgcctatcac ccgccagctt gccatctgcc
    16681 aagaattgcc aaatgcgata aaagtggaaa aaatcacgat tggattgatg atcggagcag
    16741 tcagcataaa agcaaacgct gtgtgaactg gtacgccttt tttaacaaat tgatgaacga
    16801 tcggtacgat gccgcattca catgaaggaa agaaaaatcc taagatcgaa ccagtaaaaa
    16861 tagagaagat acgattttca ggaagccatc tcttcacacg ttctggggtc aaaaatactt
    16921 gtaaggcgcc tgagataaga cagcctaata atacgaaagg taatgcttca ataacgatcg
    16981 ataagaaaat cgtactcatc tgcaaaaccg aatttggaat aaaagaaaac atgagtttcc
    17041 tcctttttat ttctagttca ttcgctttgc aaatatacgc ttttattcaa aaaaaacaag
    17101 ttgctctgta aatttttaca caactcttat tttttaccaa agcacttcaa ttgacttttt
    17161 ctgttattcc cattaaatta aggtctcata catatcactt ctatcataaa agggagaata
    17221 aaaatgaaac aaaccactaa cttgactgaa gttcaagaca tactgcaaca gtcatccgtt
    17281 gccgtcattt atctttcgat gcctaattgc agtgtttgtc acgctgttcg tccacgacta
    17341 gaggaacttc tcacacatta tgatattccg gcacttcatt tggatgcatt tgaaacacca
    17401 gaagtagcta gtaaatttga agtgctgact gcgccagttg tcctgatttt tcatcaagga
    17461 aaagaagtct ttcggcaagc acggttcatc gactttaaaa aaatccgtta tctactagat
    17521 gaactgacag cagaagatga ttccctttct tatgaagaaa tcttccgaac aaaataatcg
    17581 ttgtccaaac agaaaaaagc gattgaacaa gtgaaagttt tgccttcatt tgttcaatcg
    17641 ctttttattt ttattctgct gtattttcgg aatgcgcatt tgcgtcagaa tgcaagaaat
    17701 agatcaaggt ttgcaattca ttcgtcaaat caacattttg gatcaataca tctgacggag
    17761 cattcaatcg agcagcggta aagttcaaga ttcctttgat tcctgcttcc gttaactgat
    17821 tgactacttc ttgtgctctt tcagcgggta tcgttaaaat tgctacttcg atttgttgga
    17881 tcttgatttg ttctgacata tcttccattg gataaacggg gataccgtct acgatgcgcc
    17941 caacgatttc ctcattcaca tcaaaagcac aactgatacg tatgctatta ctttgatgga
    18001 atttgtattt tagtaaagca cttcccaagt tccctacacc gatcagcgca acatttgtca
    18061 attgatcgtc gtttaatgtc tttgcaaaaa agtgcataag atcttctaca tcataaccat
    18121 acccacgttt tcctaattcg ccaaaataag aaaaatctcg acggatcgtt gcactatcta
    18181 cttgaacggc ttcacttaat tcagttgaag acactttttt ctttccagca ttatttaaaa
    18241 tccttaaata gcgataatac aaaggcagtc ttttagctgt cgcctttgga ataatatgat
    18301 ctttcacgaa atgacctcca cgtattgtct aattacatct ttgatttctc catcatagca
    18361 gtatttatag gaaaaaatca attccattgc tcagatattg tgaaatttta ttctttattc
    18421 atttatttat catttctgca aatctcgcag tataatggaa tttatggtag actactaatt
    18481 gaaaaatagt tttttatttg aggagaattc acaaagtaga aaagaggatt ccatgatatt
    18541 attacaagcc aatcagattg ctcgccattt tggtgcagaa gtcttatttg aaaacataca
    18601 tttagaaatc agtacaggcg ctcgtgtcgc tttagttggc agaaatggcg ctggaaaatc
    18661 gactttatta aagatcattg ctggaattga agcacctgat gaaggaaaga tcgctaaaaa
    18721 taaaacagcg actctcggat acctcgctca ggacacagga cttgattcca atgaaacagt
    18781 ttggaacgag atgctgaaag cttttgaaca agttagacaa atggaagagc ggatgcgcga
    18841 gctagagatt ttcattagcg aaggtacgcc agacacacct gcttatagtt cttatttaaa
    18901 agaatacgac cggctgcaac acgatttttc agatcgaaac ggctatggtt atgaaaatga
    18961 aattcgttcc gtattgcatg ggtttaaatt cgattcttca ttttatgatc agaaaatcga
    19021 tactttatca ggcggacagc gcacacgttt agctttagct cgcatgcttt tgcaacaccc
    19081 tgacattttg attctagatg aaccaacgaa ccatttagat atcgagacac tttcctggct
    19141 tgagaattat ctgcaaagct atactggtgc attattgatt gtttcccatg accgttattt
    19201 tttagataaa gtagtcaatg aagtctatga attaagcaga agaaaaatgc gccattacaa
    19261 aggtaactat tcgaaatatc tagatttgaa agcagaacag ttagcaagtg aatggaaggc
    19321 ttatgaaaag cagcaaacag aaatccataa attagaagac ttcgttgctc ggaaccttgt
    19381 tcgcgcctct accacaaaac gagcgcaaag ccgcagaaag acattagaaa aaatggaccg
    19441 gctggatcgt cctcaagggg atgaaaaaca agcaaacttt ctattcagta tagaaaagcc
    19501 ttctggaaat gtagtcttgc aaatcgaaga tgccgctatc ggatatgata atgtcacctt
    19561 atctgaacca atcaatctag atatccgtcg tcaagaagcg attgctctag taggtccgaa
    19621 tggtatcggg aaatcgacgt tgctgaaatc aatcattcac cagatccctt ttatccgtgg
    19681 aaaagaaacg ctagggacaa atgtcaccat cggctattac gatcaaggtc aagctgatct
    19741 gcatacaaac aagacgatcc tagcagaatt gtgggatgag catcctacca ctccagaaaa
    19801 agagatccga agtgctttag gtgctttctt attttctggc gaagatgtag aaaaaccaat
    19861 ccctctgcta agtggcggtg aaaaagcacg ggttgcttta gccaagttag cgatgaacaa
    19921 agaaaatttt ttgattttgg atgaaccaac aaaccattta gatattgaca acaaagaagt
    19981 attagaaaat gcattgatcg attatgacgg cacactgctt tttgtttccc atgaccgata
    20041 tttcatcaat cggatcgcga caaaagtcgt cgaactctct gaaaatggca gcaaagtcta
    20101 tctaggagac tatgattatt atttagagaa aaagcaggaa gaagaagaaa tcgccgaatt
    20161 gttggcacaa gatgagccag aatctgctcc tgctcccacg aacaaatctg attattacca
    20221 aagcaaagaa cagcaaaagc ttatccgtag ccttcaacga aaaattactc aggttgaaga
    20281 agaaatggcg cggatcgatg aatggatcga tcagttggaa caagaaatgg tcgcgccaga
    20341 aaacctggaa gatcacgtaa aactcaatga gctgaaccaa gatttagaag ctgctagaca
    20401 agatcaagaa acaaaattga cggaatggga agaactaagc ttggaattag aagaatatga
    20461 aaattaacga aaaaaccaaa acagaaacca ttcatgctgt tttggttttt ctttttttat
    20521 tttatcaaac aaaaattcca tatttacctt tttcaccaat aagaagacca attactagtc
    20581 attctcacta aagcttctaa ataatagtaa tcaccccaaa tacaaggtgt atttacccca
    20641 ttatttttgg gcttattata aacagactgt gttaaaaagc catcttcttc ttccgttgta
    20701 gcatatcttt ctttcaattg tcgcataatt ttccaaacag cagactggta taagttcttt
    20761 tctgttttgt ctaatagacc tgattctatt aattctaata atccacaaac catgatcacc
    20821 atactagatg tatctttttc ttctctgcta gaatcatcaa agatcaaatc ccaataacaa
    20881 atggtatctt ctggcaaccg attcataaaa taattggcta atattttgct taaatcaaaa
    20941 tatttttgtt ctttcaaata acgataactt aatgcaaaac cgtaaatccc ccatgcttgg
    21001 cctctagccc aacaggaatt atcactataa ccttgatgcg tacttccata tcttggtttt
    21061 ccatctttga catccatata aaatgtatga taagttgaac catcttctct tactaaataa
    21121 tctgctgcat tttgaatatg agcctgagca gcttttttat atttttgatt tccagtttct
    21181 tcactcgccc aatacaataa tggaagattc atgcaacaat ccatgatcat tctaccttgt
    21241 tgtgaaggat cactaggatc tccccaagct tgtataactc ctgccttctc ccaaaaacgc
    21301 tgaagtaatg tttctgcagc atcaattgaa gcttccttca tttttcgatt tcccgttatt
    21361 ttataatctg ctacggttga taaagaatac aaaaaaccaa gatcgtgatg attagtgata
    21421 atttgacagt caatcctgtg gataaaatct atatctgctt tttcagctgt ttgttgaaat
    21481 aaaggatcac ccgtcttctc aaataacaac caattaattc ctgtccaaaa tccttctgtc
    21541 caaccaatat tgtctcttaa tggataacgt ccttttatag gacactcgct tgggaattga
    21601 tactgactgt tactgatacg tttgattaac ctctccgtgt gtctgcgaca acgctcgatc
    21661 tcattattcc attctttttc cattaattct ttataacatt catttatcca tttttctgga
    21721 tgtattatct cttcaaacat cactttatca agcatttccc caccagcctt tagcataatt
    21781 ttagtctttt tttattgttt ttattttgga tagttagccc ggttgtccca tcaaaatcaa
    21841 ttttgggacg attgttccat acttctaaac tataatcccg atcttgatga gcaaccactc
    21901 ctgttccgag ccaatgggtt ccttttccaa atgtaccctc taatccagga ataaaagtgt
    21961 tttcccatgt catcaagttt gtattgggca aacccataat agctgtggat tttcgtttaa
    22021 tagtatcgtc acttaaacag ataatacctg aaaagccagc ttcactcacc ctatagtttt
    22081 ccttgtcttc ttctatgatt attccttctt tctcaggaga acattcaata gcaaaccctg
    22141 tctcatatag aacatattct gtatctaatt ctactttgtg tacgcgaatc tgccagcctt
    22201 caaaaggaat aagccaagtt ctgatttttg cctgtaaagt aggttcccat gtagaggcaa
    22261 gtacctcttt cttacataca atgtctcgta cattatttcg aacgatgaac tgtccatagg
    22321 ttttgatgct ctcattctta aaactggtcg tgatatatgt gtccgctcgc tgaatcccta
    22381 gcattgaatc cattgccatt tctgctttca attgattcgc tcgcggtatc gaaaaaccaa
    22441 attgactaga ataagcaaat tttaaatatt tttcattggt caatgtatgg aaatttggcc
    22501 ctaattgtcc cgcattcaaa aaaaatgtat gtccattatc atgttgtatg agcaaattgg
    22561 cttctgaaag aagtttagtt ccttcacgaa taggcatagg catttcttta gccgtccaat
    22621 actcatggtc ttcaggtagc gccaacaata aaaaaatctt attcagccat aatggagata
    22681 acgtactatt atagggttca gtcaatgtca attgagggta ggtataacca atattcagga
    22741 tacctttttc atcaaatatt tctttttgca tccaccatct tatattccga ttgactaacc
    22801 ccttgatttc acctaattta aaaggcaaaa gatcggccca caacatagct gaccaaaagc
    22861 ttgaaactgc aaaacgataa atcatgcttc gaccaaaagg cacatttgca ccatcttccg
    22921 caaaaaaatg aatatagtct ctagaaaaat caatagctcg gttcttatat atctggctaa
    22981 tgggcttttc tggttctagt acgctataaa tcaaaccata ataatgaaat gcaaaaggat
    23041 tataataatc ttggcgacct cttgaactgt ctcgatacca gccattttga agataacaat
    23101 tttctatatt atcaaatgct tctttcagtg ctgattcttt aactggataa ccaaattttt
    23161 ggataattgt tccgacaatt atcttaaaaa attgccaatt accatctgca cattcatatt
    23221 caaaaatttg cgagaaccaa tgaataatct ggcttttttc tcctgtatct aattttttcc
    23281 ataataattg ttcatgatga tataacgcta ataacattgc gggcatttct accatacgct
    23341 ggtctctttc atgtatttct ccccaataat cgggatggga aggatctgtt ccagctttta
    23401 ttcctgatag aattaatttc aaccatcgtt catctaattg gtcaattgcg ggccccgctc
    23461 cccataaaac ccgagagaat ccttccattc ctgcaatttt atttccatat cctacccctt
    23521 gatgtatata atcaatttgt gcgccacttg ctcgataaaa cggctggagt ggttgattta
    23581 tctttttaaa taagtcaaag taatcctgtc tggtccttaa tagtggtact ggatgtttca
    23641 ttgtattttc tcctcttttt actaccaaat agctaattcc gactgttcta atcgaagtaa
    23701 gccttcaata aaaaaataat cagaatagat cagtggcaca ttataagtca gttctctatg
    23761 gtatgcttct gctccgcctc caatgatccc atcttcatcc atattccaat ttgcatagtg
    23821 cttttctgtc ttttggagta tattgatagc agcttcatga tacatttttg atcgtacaga
    23881 ggaaacatat cctgctaatt ccagaagacc gcatgcagcg catactccag cactggtatc
    23941 aatattcgaa tcttttacag caaaatcaat gagaggaata taaccagttt tttcgatttt
    24001 ctgaataaaa tttactgcta tcttttctgc tatagctaga tagtacggat tttttgtgta
    24061 acgataactt aacgcgaatc catataaagc ccatgcattt cctctgctcc aacaagaatt
    24121 ctcattgtat ccttgcccgc caagtaaccg aatatatttc cctgattctg catcaaattc
    24181 tgcgatatgc ccggttgatc catctttcct cactagatac ttagctgttg tatctgcatg
    24241 ctgccgagca attttagaga acctcggatc accagtttcc ctcgaagccc aatacagtaa
    24301 tggtaaattc atcatactat caataatcat ccaaccaggt ttatggtcat tccatgctac
    24361 taaatagttc cctgaaatat tatagcgtcc agcaagtacc gtagctgcat gtaaccctag
    24421 ttgtcgacta tgtttgtttt gagactgttg ataatgtgca atagccgatg gtaagaataa
    24481 aaaacctaca tcatgatgaa cgtctgaaaa atggtacagg gcagattcta ataattgttc
    24541 ttgttgtatt gctgcctctt tgaacttttc tcgttttgtc ttgtgaaaga gctgccataa
    24601 aataccactc caaaaaccat tggtccacca gtcgattcct ctgtccttta ttacgtctgt
    24661 atattttccg ttgtaagaaa tatatggaat tttattcccc attctgtcta attccgcatc
    24721 tatcttttca gaagctacat tcaaaatttt tcttgaccat tccttcgtct gttcattcat
    24781 ggatatcccg ccattctttt aatttttatg aatgtggagt tgctcctaga acgaactaga
    24841 tggttaacat tcgttatttt ttttacttat ttgcagctcc aatgtcattc tccttaaatt
    24901 tagactgcgc ccacacataa atcaagacca ttgggatcac tgaagttgct aaaattagca
    24961 taattgcgcc ataattaatt ggctcacctg tactagaaac cgtcatattt ttgataatta
    25021 agggaaccgt atatttatct ttatctgaaa gcatgactaa tggaaataga tattgattcc
    25081 aacttcccat aaagataagt aaaccactag taataatcgt aggtgataaa atcggtacag
    25141 cgattttcgt aaaaatcacc cactcattgg caccatcgat tctggcagcc tccatcagtg
    25201 ctgtaggaaa ttgactgcta tattgtcgca tcataaacac tgaagttgta ctggcaatcg
    25261 aagggatgat cacacctgca taagtattcg ataatcctaa acgactcata atgtaaaata
    25321 aaggaatcaa tgtggtaaaa aaaggcacca tcctagatag cataactgta ttgaacaaca
    25381 gtttattccc cataaaacga tactttgcta atgcataacc cgccattgta gaactggctg
    25441 tagctaaaac tgtactgatt acagcaataa caatcgaatt ccaaataatc aagggataat
    25501 tatatctcgc catgatggct tgccagtttt ttaatagcat atccccaaac ttgaaattga
    25561 ttccgccact taaaatttct tgattacttt gcgttgaagc tacaatcata tataaatatg
    25621 gcatcaaaaa tattaaggca ccaaacacaa taatgacgta cgtaaaaatt ctgctttgta
    25681 tcttttgttt agtcattttt atcacctccg aatttaaatt gaatagccga taatataaca
    25741 gatacaaaca ccatcgtcca ggcaatagca gaggcttttc caaaatcaac catttcaaaa
    25801 cttgttttat ataaattgac tcctacgacc aaagctgaat tttctggtcc attggaagtc
    25861 ccaaagaata tatttgggat attgaaatta cccaagccat tgatagtggc taaaactgtg
    25921 gccattaaaa tgattggctt tagcatgggg atcgttaata aaaagaattg ccgaaatttc
    25981 gatgctccat ctatttcagc actttcgtat atctctttag aaatcccttg aagaccagct
    26041 aaaaataaaa tagtgttata tcctacacta agccaaatca tcgtgatgat cattgatatt
    26101 cgtatccaaa aaggatttgt taaccactgt acaccgttaa ttcctagcgc attcaaaaat
    26161 ccattgaata acccagtcgg attgaaaaaa gtcatgaata cggaagctac agccaccgtt
    26221 gatgtaatgg ttggaatata atagacagtt ctaaaaaatc cttttccttt taactgtgct
    26281 gaattcaata aaacagcaaa gtaaatagct aatagcatcg ttattggaat tgatcctgcc
    26341 atgataatca aaacattcgt gtaagattta ataaacaatg gatctcggaa gatagctaga
    26401 taattgtcaa atccaacaaa ttcaaatgag tagccaattt tcataaaact aaaagcaaac
    26461 gagataatga tgggaatcaa aatcgtcaca ataaagaaaa taacaaaagg tgtgaccaat
    26521 ccataagcac tttttttatg agacgtatct gttgcttttt tcttgctgtt ctttattgtc
    26581 ttgcttggta acgaagattg catcatcgtt aagtcctcct aatatttttt aatcaccagt
    26641 taattttacg ccgtacttat gagcaaaatt ttctgcttct tttagatatg aattttcaaa
    26701 atcacctgtt tgccaatagt tatatgcagc ggtagtcaaa taagacgtag catctgaata
    26761 agtggctggt aaaacagtat ttgatgtatt ttcattgatt ttattcaata gttcatagta
    26821 tttttgtcct ccttagtagt caaacttttt ctcagctgct tctgtttgat aggccaatga
    26881 gttagatgta gccacgccca gctccagtgc gttggctaag cattcttcat ctgtcagcat
    26941 aaatgtaaga aattgttgtg ccacttgtgg atttttggca tctttaggga cataaaaact
    27001 tgatcctcct gatactgcac cttttcctgg attttctttt gaaaaaggaa tcagtggagc
    27061 catgcgccat ttgtcagatg catcaggaaa attcaaagac atattcgtag cataccagcc
    27121 gccagcaaca aacattgcag attcttgaaa tgctttctca gcatcttgtg caccgtagaa
    27181 atgtacaata tctgagtcga tcatttcttt gattaacttt gcagcatgtt tcgcttcttt
    27241 agtattcaga cggatatttc cttcttcatc aagcaaagat tgattctgct gggctagcat
    27301 gttgataaac aattcttggt ccccagattc tggcaaggca attccaaaac taccagtttt
    27361 ttcttttaat gcttttgcca cctggatata ttcttcccaa gaactgattt cttcaaaatc
    27421 tacacctatt tcagcaaatt tttttggttg ataataacaa agtgctgggg taaaatcact
    27481 agcccaagga ataatcgttt cattactcgt ttgttgacgc aaaatattca atttttgatc
    27541 ataaaactga tcgccatatt tgtcataaaa tccatattca tcagcactat aaaaagcatc
    27601 ttgaaatttt tccagcacac ctgctgctga agtgtctgcc atggagataa tatccggcat
    27661 atcttcgtgt gttaccaatt ttggggtgat catttgtact gttgctgttc ccgaagctac
    27721 attcacaatt tttaagtcaa tatttgctcc agttttgcgg ttatagattt ttgcagcatt
    27781 ttcaacatag tttgtcagtt tgccgtttgc gacatatact tctaatgtag tggctttttt
    27841 tatcattgga tctgcgatgt ttgctgttac ttcttcgtta gtttttccac atgcacttaa
    27901 gctgaataat agggtaatgt ttagcaaagc tatcaccaat ttgtatcttt ttttcatgtc
    27961 tatcccttct ttcttttcaa gttcttatat aaaacgcttt cacttatgta tattaacgga
    28021 attagtaaca caaatattta tataaaaaaa tattttactt gtcgttttgg cttttcattt
    28081 ttgttataat agtcattaat tataaataga aagcttttta tataaaaaga gaaaggaaag
    28141 tggttctttt gttaacaaac aaccaatcct ccaatcttgt caaaaaaaca ctaacctatt
    28201 ccatagatac ccatcttcat ttatctgctg ctccctattt tcgactacta ataaatgaaa
    28261 gcgctaattt atatgctgaa tttgcacata aatctataga actagataat ccttcccttt
    28321 tattcattga taaacagcaa gaagtccaaa tcttttctac tcaaaaggaa acacaaactt
    28381 ttatcattat ccagttttat tttgatgagc aattattagc acatattacg ggacaatctt
    28441 tttctataga tcaagaaata acatctatca ttacttttct gaaaaatgaa cagttcaaaa
    28501 ttgattatct aaaacatagt aagccagcta tttcctatga tcaggaaatc aattcttttt
    28561 tcatactatg ccaacagatg agcattcatt tgctttcctt attagttact tattgtctgc
    28621 aaaaaattca attgtcttcc ttaccaaggc attatcaaca ggaacttgct ttattgacaa
    28681 taccttcaaa tgagaaagtc attcctaaaa aaaagcatat tctttattca gatgaattag
    28741 ttgatccaat catacgatat ttagaagaac atctttcaga aaattttttg cttgaacaat
    28801 tagcccaaat tttttttatc agtccagcca gcttaaaaaa acattttaga aaagtgaatg
    28861 gaaaaagtgt tattagctat tttaaagaac taaaaatgga aaaagcaaaa cgtctcgtgc
    28921 tagaaaatga attaacctat acagaaattg cagatatatt aggatttcat tcgatccatc
    28981 atttttcttc agcttttaaa aaatatacag ggttatcccc ttcaaagtat tatctatcct
    29041 taaaatagaa aaaaattttt aggttttatc cacaaattat ttctctatat tttctgtcta
    29101 ttcaaaataa acaataattt tccggcacaa ttatttgctg ttttttacca taacatagta
    29161 cgatcaacgt agaaaaggag atgtttttat gaaagttgtt gtcattggcg gtgtagctgg
    29221 cggtccttca tttgccactc gtttccgtcg attgaatgaa gcgcacgaaa tcattatcta
    29281 tgaacgcgga gaaaatattt cttatgcaag ttgtgctttg ccttattatt taggaggtgt
    29341 gatcactgac cgtgactcgc tgatcgaacg tacaccagaa atattgaaaa caaaaaacaa
    29401 catcgatgtc tttactaaac acgaagtaac agcaatcgat ccttctacta agcgattaac
    29461 agttaaagac ctatccacaa atgaagaaat aaaaacagat tacgataagt tgatcatctc
    29521 ttctggtgct agaccagatt atccggatat tcccggagtt tttgaagcag aaaacggctt
    29581 tgtcctccgt agtgtgacgg atgcggatcg aatcaaatcg ttccttgaag aaaaaaatcc
    29641 acaacatgtc gtcattcttg gtgcaggtgt tatgggtctg gaattagctg agaatttcaa
    29701 gcatcgcggc ttagacgtga ctttaatcga tcaattgcca caagtcgctt tcccttatga
    29761 tccagaaatt gctaatttag tttatgacaa attgctgaaa gaaggattag ccgttcattt
    29821 agaaacaaga gttactgaga tccgtgataa aggtcgagaa atagtattat cagatggctc
    29881 cgtcctttct gctgatatgc taatttttgc tgttggtgtt tctccgaaga atgaagtggt
    29941 gaaagcaacc ggcatacaat tatcagatac aggtcagatc attgttgatg accagttaca
    30001 aaccaatctg ccggacatct atgcgattgg cgatattatc gaaacgacta gtgtagtgac
    30061 tggtcagccg atccaaagta tgctttccag tgcggccaat cgtcaaggac atatgctggc
    30121 agatatttta aatggtacgc ctatgcgcta tcgcggatat attggtgcag gtgtcgcaaa
    30181 aatctttgat catacagcaa gttatgcggg aatgacagaa catgaattaa aagcatcagg
    30241 cataacaaat tataaaactg tttttatcac tccctttgac catgcctatt tctatccaga
    30301 agctacaaga ttaaatctaa agctgatttt tgatgcagat agcggtcgta ttttaggtgg
    30361 acaagcattt ggagaaaaag gtgtcgataa acggatggga gaactttctg tagcaatcac
    30421 cggaaacttg acagtctttg atttgcctga tttggagttg ccttactctc caccgtattc
    30481 tactacccgt gacccgttga atatagctgg ttatgtcgca atcaatcaaa tgacgaatat
    30541 cgtagaaacg atcaaagcaa gtgatatacc cgaaaacgat ttgaaagaag cgttcttttt
    30601 agatatacgt gaacctaata aagcaccttc tggcagtatc tcggcaacaa aaaatattcc
    30661 aatgaatgag ttacgagatc gaatcaatga aatcccaaaa gataaaaaag tttacattac
    30721 tttcagaaga ggattgaata cttatacttc tgcccgaatc ttggcaggtt tgggtatcaa
    30781 agcgattttg attgaagaat aaaaaaacga ccgcggtggg tagccgcggt ctaggagtta
    30841 tttttacttg aggagtaaaa atgaaaaagt atatagggtt gttggttggt atgtatatag
    30901 aatagaagat aaatgtgaag aaattgtgca ttatttcttc acatttatta ggttatagtt
    30961 taagaaagtt taaacattgc gcaatttaat tattttagct gtttcttttc ataaaaaaac
    31021 atgttgtgac tctgagacaa atcatagtcc atagtgatgt ctctagagta actacgccta
    31081 atattacgag gcatcatatt aatctatcag cactagtacc taaactagca tccctttcga
    31141 atattttttc ttatataata aagtttgctt tcactcctct aatctcacca aatcgactgg
    31201 cttgcgttgt gtgatactag gaactgattg gtttagaacg acttttctat tgtaatcata
    31261 tttttcgcca tcctcgttgt cttctaatcc ttcaatgaat gatccctaat gtgtgtatta
    31321 aataatttaa tttttttgtt cctatttatt taa
//
LOCUS       DAZUKZ010000024        30229 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000024 DAZUKZ010000000
VERSION     DAZUKZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.024"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..369
                     /locus_tag="ACG8EF_001038"
     CDS             1..369
                     /locus_tag="ACG8EF_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFS4946379.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="ACG8EF_001039"
     CDS             549..1490
                     /locus_tag="ACG8EF_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFS4946380.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="ACG8EF_001040"
     CDS             1559..2290
                     /locus_tag="ACG8EF_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4946381.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="ACG8EF_001041"
     CDS             2434..4614
                     /locus_tag="ACG8EF_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFS4946382.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="ACG8EF_001042"
     CDS             4634..5116
                     /locus_tag="ACG8EF_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFS4946383.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="ACG8EF_001043"
     CDS             5132..5893
                     /locus_tag="ACG8EF_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS4946384.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /gene="murC"
                     /locus_tag="ACG8EF_001044"
     CDS             6024..7358
                     /gene="murC"
                     /locus_tag="ACG8EF_001044"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFS4946385.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="ACG8EF_001045"
     CDS             7665..8150
                     /locus_tag="ACG8EF_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFS4946386.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="ACG8EF_001046"
     CDS             8143..8826
                     /locus_tag="ACG8EF_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFS4946387.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="ACG8EF_001047"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="ACG8EF_001047"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFS4946388.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="ACG8EF_001048"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="ACG8EF_001048"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFS4946389.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /gene="coaE"
                     /locus_tag="ACG8EF_001049"
     CDS             12442..13071
                     /gene="coaE"
                     /locus_tag="ACG8EF_001049"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFS4946390.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="ACG8EF_001050"
     CDS             13123..13455
                     /locus_tag="ACG8EF_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS4946391.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="ACG8EF_001051"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="ACG8EF_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFS4946392.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="ACG8EF_001052"
     CDS             14281..15654
                     /locus_tag="ACG8EF_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS4946393.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="ACG8EF_001053"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="ACG8EF_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFS4946394.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="ACG8EF_001054"
     CDS             16709..17149
                     /locus_tag="ACG8EF_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS4946395.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17293..17994
                     /locus_tag="ACG8EF_001055"
     CDS             17293..17994
                     /locus_tag="ACG8EF_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFS4946396.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17991..19112
                     /locus_tag="ACG8EF_001056"
     CDS             17991..19112
                     /locus_tag="ACG8EF_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFS4946397.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19127..20359
                     /gene="pepT"
                     /locus_tag="ACG8EF_001057"
     CDS             19127..20359
                     /gene="pepT"
                     /locus_tag="ACG8EF_001057"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFS4946398.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20491..21399
                     /locus_tag="ACG8EF_001058"
     CDS             20491..21399
                     /locus_tag="ACG8EF_001058"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4946399.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21434..21706
                     /locus_tag="ACG8EF_001059"
     CDS             21434..21706
                     /locus_tag="ACG8EF_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFS4946400.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21717..22763
                     /locus_tag="ACG8EF_001060"
     CDS             21717..22763
                     /locus_tag="ACG8EF_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFS4946401.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23014..25623
                     /gene="clpB"
                     /locus_tag="ACG8EF_001061"
     CDS             23014..25623
                     /gene="clpB"
                     /locus_tag="ACG8EF_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFS4946402.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25774..26460)
                     /locus_tag="ACG8EF_001062"
     CDS             complement(25774..26460)
                     /locus_tag="ACG8EF_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946403.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26438..26932)
                     /locus_tag="ACG8EF_001063"
     CDS             complement(26438..26932)
                     /locus_tag="ACG8EF_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS4946404.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26952..28172)
                     /locus_tag="ACG8EF_001064"
     CDS             complement(26952..28172)
                     /locus_tag="ACG8EF_001064"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4946405.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28319..30154
                     /gene="lepA"
                     /locus_tag="ACG8EF_001065"
     CDS             28319..30154
                     /gene="lepA"
                     /locus_tag="ACG8EF_001065"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFS4946406.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        10133 a         5387 c         6523 g         8186 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aatattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 atacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tgggaatttt tttattccta gtctccttat tttatttgga aaatgctata attatctaga
    17281 aggagtgaag ctatgaacca tttagattta tctcagcgct tagccgcagt gggtgacttt
    17341 gtccctgaat ccgctcgttt agcagacatt ggatcagatc acgcttactt acccgttgca
    17401 ttgatattaa gaggaaaaat cgaatatgca gttgctggtg aagtcgttca aggtccttat
    17461 gaatcagcac gtaagcaagt caaaaaagat ggattagaag accgtatcac cgtacgcctt
    17521 gcaaatggtc tagatgctat ccagccacaa gatggtatta cagctgtcac gattgcgggt
    17581 atgggaggtt cactgatccg agatatccta gaagctggca agttgaatca acgcttaagc
    17641 ggacaagaac gtttgatcct tcagccaaac atcggagagc gtacattacg atcttggctg
    17701 caagaaaatc agtataaaat ccaatcagaa aaaatacttg aagaaaataa aaaaatatat
    17761 gaaatcattg tagccgaaaa agaacaaaaa ccagttactt acaatgaaat ggagctgatg
    17821 ttcgggccat ttctgcttga agagaaaagt gagatcttca aatcaaaatg gacaagagag
    17881 cttcaacaaa gaaaagctat ccgtgaacag ctcaaacaag cagctaatca agaggcacgt
    17941 caaaaacaaa tcgagcagga aatccaaatg attgaggagg ttttaaagcc atgacaatca
    18001 gcggacgcac tttcatccaa aagtttgaaa gctattgtcc tcagtggcta gctgaagaag
    18061 gagacccagt cggactgcat atcggaacat tggataagcc aatccaacga gtaatgatga
    18121 cattagacgt tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc gatttgctga
    18181 ttgctaaaca tcctccaatc tttcgtcctg taaaacggct ggttactgat cagccacaag
    18241 aaaaaatgta tgcagatttg ttgaaacatg atattgccgt ctatgcagca cacacgaaca
    18301 tggatattat ttgggatgga ctgaatgact ggttttgtga acttctcggt attgaagtgg
    18361 agagttattt agtaaagact catgagattc attataaaaa actggccgtt tatgtacctg
    18421 tggatcatgc acagaaaatg cgtgaagtat tagctgcagc tggtgctggt acacaaggtg
    18481 attataccgg tacaagtttt acctcgattg gacacggtcg atttactccg gaagctggtg
    18541 cacaaccagc gattgggaaa gtagggaaga cagaacaagt tcaagaggca aaagttgagg
    18601 ttattttgcc ggaaactatc gaaaaacaag tgattcaagc catgcggtca gctcatcctt
    18661 atgaggaacc tgcttatgat cttttcgcta tagatgaacc agtcgaaatg tttgggttag
    18721 gacgagtagg tgaattgccg caagaaattt ctatagaggc atttgtagaa caagtaaaag
    18781 aagcgttcca actagatgga ctgagaatag tccagccaaa aaatgcaaaa tcctctgtga
    18841 agcgcattgc gatttgcggt ggaagcggtg aaaaatttta tccgcaagca atcgcccaaa
    18901 gagcagatgt ctatatcacg ggagatatct attatcatac ggctcatgat atgcaaagcg
    18961 ctgggttgat tgcaattgat cccggtcatt atattgaatc gttatgcaag caacgattta
    19021 tcgagaaatt tgaatcttgg aaacaagaag aaaattggga tattaacttt ttcgtttctg
    19081 aaacaaatac aaacccattt caatttaatt aaaaggagag aatactatgt acgaaaatct
    19141 tttgccacgt tttttacgat atgtcaaaac agaaacacgc tccgatgcaa caagcaaaac
    19201 gactccatcg actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag aattagaaga
    19261 gttaggctta gctgatgttt tctataatga agataacgga ttcgttatgg gaacattacc
    19321 aagcaatgtt gatcatgaag tgcgttccat tggatttatt tcccacatgg atacagcaga
    19381 ttttaatgca gtaggagtca atccacaaat cattgaaaac tatgatggcg agtctacgat
    19441 cgtactagat gcggaagaac gctatacatt gaatacaaaa gattttccaa atttgaaaaa
    19501 ttatgaaggc cacacattga tcacaacaga tggcagtaca ttactgggag cagatgacaa
    19561 atcagggatt gcggaaatca tgactgcaat ggagatcttg atcaataatc cttctatccc
    19621 tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg gagcggataa
    19681 attcgatgtt gagcaattcg atgtagattt tgcttataca gtcgatggtg gtcctttagg
    19741 tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc aagggaaaaa
    19801 tgtacatcca gggacggcaa aagatacaat gatcaatgct ctacagttag caatcgattt
    19861 tcataatcag cttccagccg atgaagttcc agaaaaaacg gaaggatacg aaggtttctt
    19921 ccatttgatg gcattggatg ggaatcctga agaagcttct atgagttata tcattcgcga
    19981 tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc acacatatcc aagaagagtt
    20041 gaatcagcga tttgatcaag aacgaatcaa agtagatatg tatgatcaat attacaacat
    20101 gaaagaaatc attgaaaaag atatgagcat cgtggaatta gcgaaacaag caatgatcga
    20161 gcttggtatc tctccagtga tcgaaccagt tcgtggagga acagatggtt caaaaatctc
    20221 ttacatgggt attccgacgc caaatctatt tgccggagga gaaaatatgc acggacgatt
    20281 cgaatttgtt tctttacaag taatggaaaa agcaacagac gtgattgtaa aaattgcaga
    20341 attgaacaca aagagttaaa atcgaaaaag aacagcttat gacaaggcgt tcgtcccaat
    20401 gatggaatgt catctgtcta aggctaaagg gcaggacaat ggaattggtt gtcccgccct
    20461 cttttttgaa gagtaaatag gaggtaaaga atggaaaagc tgctagtttt ttataacaaa
    20521 agttcaggga aagatgaggg tgagaaatta gcacgctggt ttcaagatta tgtaaaagaa
    20581 aagcagccag aattatatgt agaactagct gaaactggcc catcgattga tgatgcggaa
    20641 ttagtcaaga aagcagaagc catagaagct gatactttag taattatagg tggagacggc
    20701 acgattcatc atatcgtcca agcgttccaa aaaacattat ctcaatacca tgtcggatta
    20761 cttccaggtg gaactgtcaa caaccttgca cgtgttcttg agattcccct caaaaaagaa
    20821 gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa tagactatgg tcaagtcaat
    20881 gatgaagtca ttatctctac actgacaatc ggaatcttag cagatacagc agtttggatc
    20941 agtcaagaag aaaaacagcg ttacggctcg tggatcttta tcaagaagtt ttttaagtta
    21001 ttgctgaaga agaaaaaata tcatttggat ttagaaacag acgaggagca ttggaaagga
    21061 aaatcccaac tgttgacagt cacgatgtct aactccgtgg gcggttttac aaattttgac
    21121 gactcagcga ccctagatga tggaaaattt catgtaacga tcgtaccttc cttagatatt
    21181 ttccgattta ttttttattt gcctagaatc atgagaggca aattctatac gatccccggc
    21241 attacttatt tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc tgtgggcact
    21301 cgaacggatg gagacacgac agatgatctt ccagttgaat tatctgttgt atcaaaagga
    21361 ttaacagttt attcaccaaa gaaagaagaa aaagcttaga aatagtagtt aacagaaagt
    21421 tggaagaaga gtaatggatg tattgttaca caatcctttg tggttttacc ttttcattgt
    21481 gaatttttac ttattttgtc tgatggggta tgataaacat caggctagaa ctagcggctg
    21541 gcgtgttcct gagaataatt tgatatttgt cagcctgtta ggcggtggtt tgggaggatt
    21601 gatctctcga aaagttttcc atcataaaac tcggaaaaag aagtttacta taggattcgc
    21661 tgcaggtgta cttgttgatt tgattctcat ttattttttc cattaaggag caggagatgt
    21721 cgtctaaagg aacgaaaact aaaatcattc tgaatctgct tttgctgatt gtgatttttg
    21781 ccattattta ttatttaatc aatcaatcat ttgcagatat ctttgccgaa ttgctgtcta
    21841 ccagtgttgt tgttttggca gcggctgtta tattagggac agtttaccaa atcgctgaag
    21901 ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg aaagatgggt
    21961 tcttgacctc tagctatatt gcgttttacc gaatcgtttc gtttggtaca ggaacgctga
    22021 tctcggagat ttatttttat aacaaaaaag gaatgaagct atcccaggga gcaggggtga
    22081 cagcactcca tatgattatg tacaaactgg cagtgatcac ttatgcatta gctggcttgg
    22141 tcatccaatt ctctcttttt tattctaatg gtccatcaat gatctggttt atcatcgctg
    22201 gtatcctttt gacgatcgtg attgttgcct ttttattggc gatgtccgtc agcttgaatg
    22261 ttcaaatatt ttttgtcagc ttatcgaata agttatttaa aagtgagcgt ttgcggaaat
    22321 gggtggatac atgcaatcag cagatctact ctttaaggga aacggtccaa accattatcc
    22381 aagacaggac tgcgctattg cggatttatc tatggaatat gttaaaactt gtattttggt
    22441 atgtacttcc ttatttgatt ctgattgaaa accatcctaa aatagatttt ctactgacca
    22501 tttcgtttat cagctttgca gtagtgcttt caggggttat tcctacgcct gctggtattg
    22561 gttcctttga atttgtctat ttgttattat tcagaccagt tgttggaaca gttgacgcag
    22621 tttcttcgct tttactgtat cgtttcagtt cctttatctt gccatttctc tatggatttg
    22681 tgtatgtgat tgctgaacgg cgaagagtga tcaaagaaga aataatagaa gtaaaaaaag
    22741 agaataaaga aaatcaaggg taggtctttt ataagatcta ccctttttat caaatcaaag
    22801 aaaaacgtta tggataagat aattttacgt ttgtttggct ctttttttat atacatacaa
    22861 tcaattttgt ttttttaaga tttatataag tcgagaaaaa aagtcaaaaa actatgtgta
    22921 tattttgacc aaaattgacc aaagcgatat aatagttttt gtaaagatca aagaaataag
    22981 tgatcttacc tttaattagg agggtatacc tgtatgaata ttgagaaaat gactacgacg
    23041 cttcaagaag cgattgctga agctcaacag attgcggtca ctcgaaaaca tcaagatatc
    23101 gacattgccc atgtatggaa aatcttttta caatctaatc attttggtcg aaatttctat
    23161 acggatgccg gattggatgt cgaatctttt gaacatgaaa ttgatcgtct actggatgag
    23221 tatccagttg tttctggtgg aaacgtccaa tatggacaaa acctcagcca gaatttcttt
    23281 cgattattga atgaagcaga ccaaatacgt gaaagcttcg gcgacgagtt tctttcaacg
    23341 gaagtcgtta ttttagcgtt gatgaaatta aaaaattatc cgttaactgt ttatttgaat
    23401 aaaaacggat tatctgaaaa agaattgcgt aaaaacatcg aagagatgag aggaggagaa
    23461 agagtgacct cacaaaacca agaagaacaa tacaaagcat tagaaaaata cggcgttgat
    23521 ttagtacagc aagtcaaaag cggaaaaatg gatccaatca tcggtcgtga tgaagaaatc
    23581 cgtgatgtca tccgtattct ttcacgaaaa acaaaaaata atcctgttct gataggagaa
    23641 cccggtgtag ggaaaacggc aatcgttgaa gggcttgctc aacgtatcgt acgcaaagat
    23701 gtgcctgaaa atttaaaaga taaaacgatt ttttcattag atatgggtgc attgatcgct
    23761 ggagcaaaat tccgtggtga atttgaagaa agactaaaag cagtcttgaa agaagtcaaa
    23821 aaaagtgatg gacgtatcct cttgttcatc gatgaaatcc acaatatcgt aggggcagga
    23881 aaaacagaag gaagtatgga tgccggaaat ctgttgaaac caatgcttgc tcgcggtgaa
    23941 ttacatttga tcggtgccac aactttagat gagtaccgtc aatatatgga aaaagacaaa
    24001 gcgttggaac gtcgctttca aaaggtatta gtcaaagaac caacagtgga agatacgatc
    24061 agtattctgc gtggattaaa agaacgtttt gagatccacc atggtgttaa catccatgac
    24121 aacgcattag tagcagcagc tactttatct gaccgttata ttactgatcg gttcttaccg
    24181 gacaaagcaa tcgatttgat cgatgaagcc agtgcaacga ttcgggtaga aatgaactct
    24241 atgccaacgg aattagatca agttactaga cgtttgatgc agttggaaat cgaagaagct
    24301 gcactgaaaa aagaatcaga tgatgcaagt aaaaaacgct tgaaaaattt gcaagaagaa
    24361 ttagctgaac ttcgtgaaga agcaaatgcg atgaagatgc agtgggaaac agaaaaagaa
    24421 gaagtcaatt ctgtttcagc aaaacgtgct gaaatcgata aagcaaaaca cgaattagaa
    24481 gatgcagaaa ataactatga tttagaacgc gctgctgtat tgcgtcatgg tacgatacct
    24541 caattagaaa aagaattgaa ggaattagaa gctaaagcaa aagacagcga gattaagatg
    24601 gttcaagagt ccgttaccga aaatgagatt gctcaagtcg ttggcagatt aacgggtatc
    24661 cccgtaacga aacttgtaga aggtgaacga gaaaaactaa ttaaattgaa tgaaacactt
    24721 cataaacgag tgattggtca agatgaagca gttgatgcgg tcagtgacgc ggtaattcgt
    24781 tcacgggctg gattgcaaga cccaaatcgt cctctaggtt ctttcctttt cctaggacca
    24841 actggtgtcg gaaaaaccga attagcgaaa gcgttagctg agaacttgtt tgactcagaa
    24901 gatcatatgg tacggatcga tatgagtgaa tatatggaaa aacatgctgt gtctcgtcta
    24961 gtcggagctc ctccaggcta tgtaggttac gaagaaggtg gtcaattaac ggaagctgtc
    25021 agacgaaatc cttatacaat cgtcttgttg gatgaaatcg aaaaagcgca tcccgatgtc
    25081 ttcaatatct tgttacaagt cctagatgac ggacgcttga ctgattcaaa agggcgagta
    25141 gtagatttca aaaatacggt tctgatcatg accagcaata tcggttcaca gttactgctg
    25201 gaaggcgtga ctgctgatgg aacgattcca gaagcagtgg ctgaacaagt caacacgcta
    25261 ttacgtggaa acttcaaacc agaattcttg aaccgaatcg acgacacgat tttatttaca
    25321 ccattgagcc tagacaacgt gaaaggaatc gttgacaaaa tggtggcgca attggcacaa
    25381 cgtctggaac atcaagaaat cctgctgaca atcagtgatg aagccaaaac gtggattgct
    25441 gaaaatgctt atgaaccagc atatggtgca agaccattga aacgatttat cactaaggaa
    25501 gtagaaacac cactagctaa agaaatcgtt gcaggacatg ttatgcctaa atcaaaagta
    25561 acgattactt tacttgatgg acaacttcat ttcaaaacag aagagctaga agaaatcgtc
    25621 taatcttcca ttgagactat aaaagaaaaa aggggtctga cacaactagt gtcagacctc
    25681 cttttttcaa ataaacagtg ttccagaagt agtttcttcg aaaaataagc cgaaatttct
    25741 tgaagctgcc cgcttctgtc acaacctctt ttattaggta tttttcactt tttctaattt
    25801 ataactttca aagtaacgct catctttttt actttgagac catgcaaaca atcccattaa
    25861 ggcagcacct atcgtatttg tcagcaagtc gcccatcgta tccatcaatg ctgctcgtcc
    25921 gacaaacagt gtgccatcag aagcagcaaa acgttgaaga ttcatgccta agaactggtc
    25981 gcataagaat tcccagaact cccaaaacac gccgcaaagt ccagcaaacg caaagccgaa
    26041 taagaggaac aaccaaggac tggtttttga tatctcagca tctttcatca agtaaccaat
    26101 caatccatag ccaagagctg tcaggaccat tggactcacc gcatgtaaga ttttgtccca
    26161 aaaagagatg atactgatca aatgcatccc tgtgcctaaa aagacagaga tgaacaagaa
    26221 aaaccagtaa aacaaaacga ttgctgctgg tattttaagt ttgaaaactt tcaaaatgac
    26281 ttgtggcatg aaaatcaatg caatccctaa taaagattcc acgataaatg tgattccttg
    26341 agaagtcgaa tatttttgtg tgataaattc ataaaaatta tagcagacaa cgaatacacc
    26401 aaaagcaagt aaagctattt gcaccttttt gatttcttta atcactgaaa gtccctccat
    26461 ctaaaaatct ttttacttct gaaagcaact caatttctga agaaaccact tttccgttca
    26521 atttgatcaa acctattgta tataagttca ggtagtgaaa ttgattttca gctgtttctt
    26581 gtagtgcagc gatttttttc gggtgggttc cgccttgctg tcgagtatca gtatataagc
    26641 cgatcactgg gatgcctttc gcataagcca caccgatttc cgatgctacc ccggcatcga
    26701 tcgtcagccc atctagtaaa gcaatcatta aatcactagc caacactttt tctgtgtcag
    26761 caagagcaat cattcgactg tctgcataag cagttttgtc attgattgct tcattttctt
    26821 gaggaagata gatcgaaagc tcagaagaga gctctctgat ttctttaact aactgttggt
    26881 tatataacaa atcactttta gcaaacattg gtgcggcaaa ataaattttc atctaaacat
    26941 catccttttt atcaaaaata atcgctaaag taagcttcca tgtttggaat gatacgctct
    27001 agtgacttca gtgtttcttc atctgtttct aaccgttcga ttcttgccaa ataagaagca
    27061 cggcgataat tcatcatcaa gcaagtcaat gtttggatat ccagtcgaac ggattttcct
    27121 ataggttcat ttaagacacg gacttgtccc tgttcatccc atgttaaagc gaagacacca
    27181 ttattccaag gagcgacagg atcttcaatg atgaaatgaa aagcatcagc tgttccaaca
    27241 aacggaaacc gttgaaggaa ttcttttaca tcaacgatcc gagccataaa atatggttcg
    27301 atctgctctt tgatctgact atcttcaatc aaaaatgcta aaggctcatt tttataaata
    27361 tctccatgta cccagtagat catggaaaaa tgtgcggaaa taaagttcca taagccattt
    27421 cgagcttctt gatcaagata gaacatttct tttacgtgaa atatttcttc agctacccag
    27481 tagaacagaa cacctcttgg ttcatggttg ctatcgtagt agacagcggc tgtgcgctca
    27541 tcttcatttt cgaaacgcca atactcttcc caatgaaaat cactgcggaa taaagcacca
    27601 tggttttgtt gggcaaattt gtcataaacg gaaaatacgt ctggatgatc aactggtaat
    27661 cgctcaacca taccacttac tgggatttgt ttaggtagct gtgtatctct gattttaaag
    27721 gaaagtttat cagacatgat ttcccatcct ttacgccgat agtaggggat actgtaagga
    27781 tacaaataag agatccattg cttattcttg cgcatattat ccaatgcaac gataattaga
    27841 tcttgcatca atccatggtt agcatattct ggataagttc caactccagt cacaccaccc
    27901 attttataac gtgcaccgtg aatattgact tcacaaggat agatagcgat ttgagagatc
    27961 agtttatttt cgtggaacca tccgaacact ttcgatactt ccaaaatcgg ttttttggaa
    28021 cggataaaag ctcgtttatt ctcaaacccg ctttcctcga tgtctgcttc agtcacttgg
    28081 aacacatagg aaagcaattc attgaattga tcgatatgtt cttgagtgac cggttttaat
    28141 tcaagatttt ctctaaaatc tttttgatcc attttttcat ctccatttat tagcttgata
    28201 acagtatagc aaaattctac aaaaaaaact tctatagctg actaattaaa aactctttaa
    28261 attactgctt ttcgttggta taatggaaag tgctgattat atgaaaagag gagacgctat
    28321 ggatataaag aaaatgaaag aacgccaaga gcagattcgt aatttttcga tcatcgctca
    28381 tatcgaccat gggaaatcta cattggccga tcgtatttta gaaatgacgc acactgtgac
    28441 gtctcgcgaa atgcaagatc agctgttaga ctcgatggat ttggaacgtg aacgtggaat
    28501 cacgattaaa ttaaatgctg tagaactcaa ttacacagcg aaaaatggag aaacatatat
    28561 ctttcacttg attgacaccc cagggcacgt cgacttcacc tatgaagtct ctcgaagcct
    28621 tgctgcctgt gagggtgccg ttttagtggt cgatgcagca caaggaatcg aagcacaaac
    28681 attggccaat gtttatttag cgttagacaa tgatttggag atcctgccag tcatcaataa
    28741 aatcgaccta cccgcagcag atcccgaacg tgtccgtcaa gaaatcgaag acgtaatcgg
    28801 aatcgatgct agtgaagctg tattagccag tgccaaatca gggattggga ttgaagatat
    28861 tctcgagcag atcgtggaat atgttcctgc accaagtgga gacttagatg caccgctaaa
    28921 agctttgatc tttgactcta tttatgatag ttaccgcggt gttgttctaa atgtccggat
    28981 cacggacggt atggtcaaac ccggagacaa aatcaaactg atgagtaatg gcaaaacatt
    29041 tgatgttaca gaggtcgggg tcttctcgcc aaaagcagtt gctagagact ttttgatggt
    29101 cggtgatgtc ggttatatca cagcaagtat caaaactgtc caagatacac gagtagggga
    29161 tacagtaacg ttagcagaca atcctgccgc tgaagcttta ccaggttacc gaaaaatgaa
    29221 tccgatggtt tattgtggtc tttatccaat tgatacttct cgttacaacg atttgcggga
    29281 agctttagaa aaactgcaat tgaatgatgc tgctttacaa ttcgaaccag aaacctctca
    29341 agcattaggt tttgggttcc gctgtggatt cctcggactt cttcatatgg atgtcgtcca
    29401 agaacgcttg gaacgggaat tcaatctaga attgatcacg actgcaccat ccgttattta
    29461 ccatgttaat aaaacggatg gtacgactgc tacagtagat aacccggcag attttcctga
    29521 acctgtaacg attcaggatg tagaagaacc attcgtcaaa gcacagatca tggtcccaaa
    29581 tgattttgtc ggtgcagtaa tggaactttc tcaacgaaaa cggggagaat tcattacgat
    29641 ggattattta gatgattacc gagtaaatgt cgtctataat attccgcttt ctgaaattgt
    29701 ctttgacttt ttcgacaaat tgaaatcaag cacgaaaggc tatgcctcat tggattatga
    29761 aatgtctggt tatcaaaaaa gcaagttagt gaagatggac atcttgctga atggagaaaa
    29821 agtcgatgca ttaagcttta tcgttcaccg agactttgcc tatgaacgtg gaaaagcaat
    29881 cgttgaaaaa ctgaaaaaac taatcccacg ccaacaattt gaggtgccaa tccaagcagc
    29941 tattggacaa aaaatcgttg cccgttcaga catcaaagcc ttgcgtaaaa acgtcttggc
    30001 taaatgttat ggtggagatg tttctcgtaa acgtaaactc ttagagaaac aaaaagaagg
    30061 gaagaagcgg atgaaacaaa tcggctccgt cgaagttccg caagaagcct tcatggccgt
    30121 tctgaaaatg gacgaggacg aaccgaagaa atagcaaggg tttgtttgaa aacagtgtaa
    30181 aaaataaacc atgtttacat ggatttgcca ccggtttgcc accggtggt
//
LOCUS       DAZUKZ010000025        28562 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000025 DAZUKZ010000000
VERSION     DAZUKZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.025"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            36..848
                     /locus_tag="ACG8EF_001066"
     CDS             36..848
                     /locus_tag="ACG8EF_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFS4946407.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            845..1645
                     /locus_tag="ACG8EF_001067"
     CDS             845..1645
                     /locus_tag="ACG8EF_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS4946408.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1806..3074
                     /gene="rseP"
                     /locus_tag="ACG8EF_001068"
     CDS             1806..3074
                     /gene="rseP"
                     /locus_tag="ACG8EF_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFS4946409.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3142..4851
                     /locus_tag="ACG8EF_001069"
     CDS             3142..4851
                     /locus_tag="ACG8EF_001069"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFS4946410.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5063..9421
                     /locus_tag="ACG8EF_001070"
     CDS             5063..9421
                     /locus_tag="ACG8EF_001070"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFS4946411.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9566..10039
                     /gene="rimP"
                     /locus_tag="ACG8EF_001071"
     CDS             9566..10039
                     /gene="rimP"
                     /locus_tag="ACG8EF_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFS4946412.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10062..11237
                     /gene="nusA"
                     /locus_tag="ACG8EF_001072"
     CDS             10062..11237
                     /gene="nusA"
                     /locus_tag="ACG8EF_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFS4946413.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11259..11552
                     /locus_tag="ACG8EF_001073"
     CDS             11259..11552
                     /locus_tag="ACG8EF_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFS4946414.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11549..11860
                     /locus_tag="ACG8EF_001074"
     CDS             11549..11860
                     /locus_tag="ACG8EF_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFS4946415.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11873..14179
                     /gene="infB"
                     /locus_tag="ACG8EF_001075"
     CDS             11873..14179
                     /gene="infB"
                     /locus_tag="ACG8EF_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFS4946416.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14206..14553
                     /gene="rbfA"
                     /locus_tag="ACG8EF_001076"
     CDS             14206..14553
                     /gene="rbfA"
                     /locus_tag="ACG8EF_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFS4946417.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14710..15166)
                     /locus_tag="ACG8EF_001077"
                     /pseudo
     CDS             complement(14710..15166)
                     /locus_tag="ACG8EF_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            15331..17693
                     /locus_tag="ACG8EF_001078"
                     /pseudo
     CDS             15331..17693
                     /locus_tag="ACG8EF_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            18061..18984
                     /gene="truB"
                     /locus_tag="ACG8EF_001079"
     CDS             18061..18984
                     /gene="truB"
                     /locus_tag="ACG8EF_001079"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFS4946418.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18988..19929
                     /gene="ribF"
                     /locus_tag="ACG8EF_001080"
     CDS             18988..19929
                     /gene="ribF"
                     /locus_tag="ACG8EF_001080"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFS4946419.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20281..21066
                     /locus_tag="ACG8EF_001081"
     CDS             20281..21066
                     /locus_tag="ACG8EF_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4946420.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21079..21930
                     /locus_tag="ACG8EF_001082"
     CDS             21079..21930
                     /locus_tag="ACG8EF_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS4946421.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22054..22506)
                     /locus_tag="ACG8EF_001083"
     CDS             complement(22054..22506)
                     /locus_tag="ACG8EF_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4946422.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22971..23339)
                     /locus_tag="ACG8EF_001084"
     CDS             complement(22971..23339)
                     /locus_tag="ACG8EF_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFS4946423.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23489..23971
                     /locus_tag="ACG8EF_001085"
     CDS             23489..23971
                     /locus_tag="ACG8EF_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFS4946424.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            24008..25018
                     /locus_tag="ACG8EF_001086"
     CDS             24008..25018
                     /locus_tag="ACG8EF_001086"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFS4946425.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25133..25846)
                     /locus_tag="ACG8EF_001087"
     CDS             complement(25133..25846)
                     /locus_tag="ACG8EF_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946426.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(26019..26626)
                     /locus_tag="ACG8EF_001088"
                     /pseudo
     CDS             complement(26019..26626)
                     /locus_tag="ACG8EF_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            27110..27337
                     /locus_tag="ACG8EF_001089"
     CDS             27110..27337
                     /locus_tag="ACG8EF_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFS4946427.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27353..28519
                     /gene="hemW"
                     /locus_tag="ACG8EF_001090"
     CDS             27353..28519
                     /gene="hemW"
                     /locus_tag="ACG8EF_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFS4946428.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
BASE COUNT         9690 a         4907 c         6102 g         7863 t
ORIGIN      
        1 atagtgggaa tcttttaagt ataggaggaa aaagaatgtt acgttttttt ccgcaaaaaa
       61 acaaatatat tcaagaaaaa agcgaatttc aatttaatac cgatggacca attcctcagc
      121 acattgccat catcatggac ggcaatggtc gttgggcgca aaaccgtcgt ttgccacgag
      181 ttgctggcca taaagaaggc atggaaacag taaagaaagt cacaaaaaaa gcatcaaggc
      241 ttggagtgaa agtgttgacg ctttatgcct tttcgactga aaactggaag cgaccaaaag
      301 atgaagtaag ttttctcatg caactacctg tcgacttctt tgatacgttc gtaccagaat
      361 tgattaaaga aaatgtcaaa gttcatgtca tgggttatga aaacgtattg ccagaacata
      421 cgcaagatgc tgtaagacgt gcaattgagc aaacaaaaaa caatactgga atggtattga
      481 attttgcttt gaattatgga agcagagcag agatcgtgac tgctgtccaa gaaattgccg
      541 aagaagtagc aaaaggagaa atacacgcag aagaaatcga tgatgaattg attgctaagc
      601 atttaatgac aggtttcttg cctaaagagt tacaagatcc tgagctgatg atccgtacaa
      661 gtggagaaga gcgaattagt aattttctgt tatggcagat cgcatatagt gagctgtatt
      721 ttacaaaagc gctttggcct gattttgatg gtgcgcatct tgaagaagca atcgcctctt
      781 atcaaaatcg agaccgtcga ttcggcggtg taaaaaaaac agaagatgag aaaggggatc
      841 agtcatgaaa caacgtgtgg tcacagcagc cgtggcgcta gtcttgttta ttccgattat
      901 atggatcgga ggcatagcgg ttgagcttac agcagcaatc ttagcagtaa taggagttta
      961 cgagctgttt cggatgaaag gattgacact cttaagtttt gaaggagttt tatccgccgt
     1021 tggtgcagtg ttacttgttt tgccaaaaga aagatggttt ttctttcttc cagagaatgt
     1081 aagtacattt atgttatttt atcttatagt tatgattctt ttaggaacat cagttatttc
     1141 taaaaatacg tatacgattg atgaagcagg ttttcctgtg cttgtcagct tgtatgtggg
     1201 aataggtttc cagaattttg tcaatgcacg aacagaaagc ttggctgttt tgatatttgg
     1261 tctatttatt gtttgggcaa ctgatatcgg agcatatatg gttggtcgac gttatggtca
     1321 gcataagtta agtcctgata tttcgcccaa caaaacgatt gaaggtgcgc tcggtggtat
     1381 tggtagtgct gttgtagtcg catttcttta tttattgcta tacccagcaa aagaactatt
     1441 taatcatggt atgtttgtca tgcttctgat gacgattatt ttttcagcag tcggacaatt
     1501 tggtgatttg gtcgaatctg caatcaaacg tcattatgat gtgaaagact cgggaaatat
     1561 cttaccaggc catggtggaa tcttagaccg tttcgacagt ttgctgtttg tatttcctat
     1621 catgcattta tttggcatta tttagtaagt atacctgcgt ttctttgtga atttcataaa
     1681 agacgcaggt ttttattttt cattatattg atactattgg tgtctttggt atactatggt
     1741 aaaattatag tgttttatcg ttgttttata aaaaacacaa aaaacaagta aaggagaaaa
     1801 aagctatgaa aacgattctg acatttatca tcgtttttgg tatattagtg attgttcatg
     1861 agtttggtca tttcttcttt gcgaaacgat caggaatcct cgttcgcgaa tttgctatcg
     1921 gtatgggtcc gaaaatttat gggcatcaag caaaagacgg gacaacatat acgcttcgcc
     1981 tattacctat tggcggttat gttcgaatgg caggcaatgg ggatgatgaa acagaaatgg
     2041 caccaggaat gcctctttct cttttactaa attctgacgg catcgtagag aagataaatt
     2101 taagtaagaa aatccaattg acaaatgcta tcccgatgga attgagccgt tatgatttag
     2161 aagatgagct gactatcact ggttatgtga atggagacga aacagaagta gtaacctacc
     2221 ctgttgatca cgatgcgacg attattgaaa acgacggaac agaaatcaga attgcgccaa
     2281 aagacgtaca gttccaatcg gctaaattgt ggcagcgtat gctgaccaac tttgctggac
     2341 ccatgaataa ctttattttg gcaatcctgt tattcatcat tctggcattt atgcaaggtg
     2401 gtgttcaagt gacgaacact aatcgcgtag gagagatcat gcctaatgga gctgcggcag
     2461 aagcaggctt aaaagaaaat gatgaggtag tcagtgtgga cggaaaagaa atccattcat
     2521 ggaatgatct gacaacagtc attacgaaaa atccggataa aacattagac tttaagatcg
     2581 aacgagaagg tcaggtgcaa tctgtcgacg tgacaccgaa atctgtagag tcaaatggcg
     2641 aaaaagttgg ccaacttggt ataaaagccc caatgaatac aggttttatg gataagatca
     2701 ttggagggac gcgtcaagct ttcagcggtt cattggaaat tttcaaagcc ttaggatcac
     2761 tatttacagg tttcagttta gataaattag gcggacctgt catgatgtac caattgtctt
     2821 cagaagcagc aaatcaaggt gtaacaactg ttattagttt gatggcgctt ctttcaatga
     2881 atctcggaat cgtcaatctg cttccgattc ctgccttaga tggcgggaaa ttagtcttga
     2941 atatttttga agggattcgc gggaaaccat taagtcaaga aaaagaagga atattgacat
     3001 tagctggctt tggattttta atgttgttga tggtgctagt tacatggaac gatattcaac
     3061 gattcttttt ctagaaaaag ttgacaaata gggtataata acaaacgata actattttga
     3121 ggaaataaag gagtatatag aatgagacaa tcaaaaatgt tgattccaac attacgcgaa
     3181 gttccaaatg atgcagaagt attaagccat caaatcttgc taagagctgg atatatccgc
     3241 caagtatcag cgggtattta ttcatatctg ccattagcaa atcgtgtatt ggaaaaactg
     3301 aaacgcatta tgcgggaaga atttgaaaaa atcggagcgg tagaaatgct gatgcctgcc
     3361 atcttgccag cagaactttg ggaagaatcc gggcgttacg aaacatatgg accaaacttg
     3421 tatcgtttca aagaccgcaa tgatcgaaaa atgatccttg gaccaactca cgaagaaact
     3481 tttactgatt tgatgcgtaa tgaaatcaat tcatataaga aattaccttt aaatctttat
     3541 caaatccaac ctaaatatcg tgacgaaaaa cgacctcgct tcggtttgct tcgcggaaga
     3601 gagtttatca tgaaagatgc gtattctttc catgcaacgg aagaaagttt ggacgagaca
     3661 tataaagatt acgaaaaagc ctacaccgaa gtattcaagc gttgtggctt agaatttcgc
     3721 tcgattatcg gagacggtgg cgcaatgggc gggaaagact caaaagaatt catggcgatc
     3781 tcagaaatcg gggaggatac gatctgttat tcgactgaaa gtgattatgc tgcaaacttg
     3841 gagatggcta caagctacta tgtatcaaaa aaatcgcatg aaacacagct tgaattggag
     3901 aaagtagcta cacctaacgt aaaatcaatt gacgaagtag cagatttctt ttctgttgaa
     3961 cctcaaaaaa tcatcaagtc tgtattgttc attgcggatg aacaacctgt ccttgttttg
     4021 gtacgcggag accatgaagt caatgacgta aaactgaaaa actttttatc tgctgacttt
     4081 ttagaagaag caacagaaga agaagcacaa aaatatctag gtgctgagtt tggttctgtc
     4141 ggaccagtcg gtgtttcaga tgacgtaaaa gtatatgcag accgttatgt ccaagattta
     4201 gcgaatgcca ttactggagc gaacgaaaca ggattccact ataaaaatgt gaatccagaa
     4261 cgcgatttca ctgtactttc ttatgaagat cttcgttttg tacaagaagg agatccttca
     4321 ccagacaaca atggtgttct tgcctttact cgaggaatcg aaatcggaca tatcttcaaa
     4381 atcggcactc gttacagtga atctatggga gcaactgtac tagacgaaaa tggacgagaa
     4441 aaatttgtga ttatgggctg ttatggtatc ggtgtaagtc gtttattatc agcaatcgta
     4501 gaacaacatt cagatgaaca aggaatcaac tggccaaaag gaattgcgcc atttgatttg
     4561 catatcgtcc aaatgaatct aaaagacgaa taccaaacaa acctgacgaa tgaactagaa
     4621 gaagcgatga caaaagctgg ctatgaagta ctggttgatg acagaaatga acgcgcagga
     4681 gtgaaattcg cggattctga tttgattggc tgtccaatca gaatcactgt cggtaaaaaa
     4741 gcagtggatg gaatcgtaga agtgaaaatc aagaaaactg gcgaaatggt tgaagtacgt
     4801 aaagaagaat tggtgaatac tttaccaatc ctcttgaacc aaggcgaata ataagcgtta
     4861 aaacacagaa aataaaaata gctgaatcag ctgtttttga caatctgatg attcgctttt
     4921 aaaattggaa ctgcgatttg aatcgcagtt ccaatttttt aatgtaaaac tcagaaaaaa
     4981 cgagtatact aaaaggtact gaaaaagaag aggataagat gcgatcaatt gcattgtttc
     5041 tcatcagaaa gggcgtatat ctttgtcaga aaaacgagat ttatttgaaa aactgttgga
     5101 acaaatccaa ctagaggaag cagaaaaaaa tcatccactg cttactgctg gtgaaatgga
     5161 tagtgtagtg gtccatcgta aatcgcgttt atgggaattt acgctgcatt tttccaaaat
     5221 tttgcctatt atgctatatc ggtcattgac tcagcatctg actatcgctt ttaaagatat
     5281 tgcacaagtc aaactgaata tcttagcaga tgaccagaca ttcgatgaac agctcttgca
     5341 agattactgg gcccaagctt tggaaaatca gcaatgcgat acaccactag cacagcaagt
     5401 attgaaaacg caggtacctg tgataaaaga tcgtaaagtc atcttgccaa tcgacagtac
     5461 aggagctatt tcttatttga agcaacaata cttgccatta gtagaagagt tgttcgtcag
     5521 ttacggattc ccaaaatttc gtatcgaacc agaagtggat gagcagcaag cggaacgagt
     5581 attgaaactt tttgaagaac gcaaacaaga acaggcagaa gcctttatga agcaagcagc
     5641 tgaaagtcta gttgtccacg aacaaaagaa aaaggaaaga aaagagcaat cgccagctct
     5701 tgaaggaccc atccacatcc agctaggacg aaatattcca gccgatgaac caacgacacc
     5761 aatgatcagt atcgttgaag aagaacgaag agtcacactt gaggggtacg tattcgacaa
     5821 agaagtcaga gaactacgtt caaaacgcaa gatcttgact ttgaaaatca cagattatac
     5881 ttcatcattt atcgtcaaaa aattctcgaa cggcgaaaaa gatgaacaag tatttgatgc
     5941 gatcagtgtc ggtagctggt taaaggtacg aggaagtatt caagaagata cgtttgtccg
     6001 tgatttggtc atgaatgcgc aagatatcat agaagtcaaa catacacctc gcaaagatta
     6061 tgctcctgaa ggggaaaaac gagtagagct tcatgttcat agcaatatga gtacgatgga
     6121 tgcaacaaac agcatctctg atcttgtggc acaagcaggg aaatggggac accgagctat
     6181 cgccattaca gaccacggtg gtgcgcaagc ctttccggaa gcacatagtg ctggaaaaaa
     6241 agcaggtgtg aaaatcttat acggcgtgga agcgaatgtc gtagatgatg gtgtcccaat
     6301 tgcttataat gatgcacatg aagcattgag tgaagccaca tatgtcgtat ttgacgtgga
     6361 aacgacagga ttgtctgcag tatatgacac gatcatcgag ctagccgcag tcaaaatgta
     6421 taaaggaaat gtgatcgaaa gctttgatga atttatcgat ccaggacatc ctttgtcacg
     6481 aacaacggta gatctgactg gtatcacgga tggaatggtt cgaggatcaa agtcggaaga
     6541 agaagtattg cggatgttcc ttgaattttc aaaagataca attttggtag cccacaacgc
     6601 ggcttttgat atggggttct tgaatactag ctacgcaagg tatggtattc ctgaagcggc
     6661 caatccagtc attgatacgt tggaattagc tagatatctg tatcctcaat tcaaacgttt
     6721 cggattaggt gtgttatcta aaaaatttgg tgtcagcttg gaacagcatc accgagcaat
     6781 ctatgatgct gaagcaactg gtcacttagc atggatcttt gtgaaagagg caatggacaa
     6841 tcataacatg ctttatcatg atcagctaaa tgagcatatc ggagaaggag attcttacaa
     6901 aagggctcga cctttccatg taacgatttt agcaaaaaat caggcaggac tgaaagattt
     6961 gttcaaactg atttcaatgt cgaatgtcga atacttcgaa cgcgtcccac gaataccacg
     7021 ctcccaattg aaaaagatgc gtgaaaatct attgatcggc tcagcttgtg ataagggaga
     7081 aatattcgaa gccatgatgc aaaaaggggt agaagaggca agaaaccgag caaaattcta
     7141 cgattatatt gaagttatgc caaaagcggt ctatgcgcct ttgatcgagc aagaattggt
     7201 aaaaaatgaa catgatttgg aagaaattat ccaaaatttg gtagaaatcg gaaaaagttt
     7261 ggataaaatc gttgttgcaa caggtaatgt acattatctg aacgaagaag atgccattta
     7321 ccgaaaaatt ttgatcaatt ctatgggcgg agcgaatccg ttgaatcgtc atagtttgcc
     7381 ggatgtccat ttccgaacaa ccgatgagat gctaactgca tttcactttt taggggaaga
     7441 aacagcgaaa gagatcgtag tagaaaatac gaataaaatc gcagatatct gcgaagaagt
     7501 gattccagtc aaagatgaac tttatacacc gaagattcca ggttcagaag atgaaatatc
     7561 agaattgagt tacacaaaag caaaacaaat gtacggagat ccgttacctg agatcattca
     7621 aaaacgatta aaaaaagagt tgaattctat taatggtaat ggcttctcgg ttatttatct
     7681 gatcgcccaa aaactagtgc ataaaagtaa tgaagatggc tatctagtag gttcacgtgg
     7741 ctctgttggc tctagctttg ttgcgacgat gacaggaatc actgaagtta atccgttagc
     7801 ccctcattat tattgtccag agtgccaata ttccgagttt tttgaagatg gtacgtatgg
     7861 ttctggattt gacatgccag aaaaacaatg tccaaaatgt ggtgcacgct taaacaaaga
     7921 cggtcatgat attccatttg agacattttt aggattccat ggagacaaag tgcccgatat
     7981 tgatttgaac ttctcaggtg attatcaagc agaagcccat aactatacga aagtattgtt
     8041 tggcgaagac tatgtttacc gtgccggaac gattggtaca gtcgccgaca aaacagcata
     8101 tggctatgta aaaggatacg aaagggacaa taatctgcag tttcgaagtg cagaagtcga
     8161 cagattagca aaaggcgcta ctggagtcaa acggacaacc ggccagcatc caggggggat
     8221 catcgttatt cccgattata tggatgtcta tgattttacg ccgatccaat atccagcaga
     8281 cgatcaaaat tcggaatgga aaacgactca ttttgatttt cactcgattc acgataatgt
     8341 attgaaatta gatattctag ggcatgatga cccaactgtt attcggatgc ttcaagattt
     8401 atcagggatc gatccacaaa cgatccctac ggatgacccg gaagtgatgc ggatatttgc
     8461 cggtcctgaa gtacttggag taagtcagga acaaatttat tccaaaacag ggacattagg
     8521 tattccagaa ttcggcactc gttttgttcg agggatgcta gaagaaacgc atcctacaac
     8581 ttttgcagaa ctactgcaaa tttccggact atctcatggt acagacgtat ggttaggaaa
     8641 tgcggaagaa ttgattcgac gaggagatgc gacactggcg gaagtaatcg gctgtcgtga
     8701 tgatattatg gtttatttga tccatgctgg attagatagc gggatggcat ttaaaatcat
     8761 ggaaactgtt cgtaaaggac aatggaacaa gatccctgat gaattgcgag aaacctatct
     8821 atcggcgatg aaagaaaata atgtgcccga ctggtatatc gattcttgtt caaaaatcaa
     8881 atatatgttt ccgaaagccc atgctgcagc ttacgtattg atggctcttc gtgtcgctta
     8941 tttcaaggtt tatttcccta ttttgtatta ctgtgcatac ttttctgtac gtgcagacga
     9001 ctttgatcta gtctccatgt gcaaaggaaa agatgctgtg aaacaagcaa tgaaagaaat
     9061 cacggacaaa ggattagatg cttccgtaaa agaaaagaat caactgacag tgctggagtt
     9121 agccaatgaa atgctggaac gaggattcaa atttggaatg attgatttgt ataaatcgga
     9181 tgccgttaac tttgttattg aaggtgatac gttgatcgca ccatttagag ctgtcccaag
     9241 tttgggaacc aacgtggcaa aacaaatcgt tgaagcaaga aaagacggtc catttttatc
     9301 caaagaagat cttgccaccc gcggtaaagt ttcaaaaaca ttgatcgaat atatgaacga
     9361 caatggcgta ttgaaagatc taccagatga aaatcaatta tcgttattcg atatgctata
     9421 agcatctgtt ttcctgtctg cttgtaattt taagcagagt atggtatagt tcttataggt
     9481 aatgatacta actgtgagtg agcggatttt tcgctcactc tttttaatgg aattacgcac
     9541 ttaatttttt aaggaggcga aacttttgag tagcgtcgtt gaaacagtca cagaaatggt
     9601 gacaccgatt ttagatgaac aaaattttga acttgtcgaa gtcgaatttg taaaagaagg
     9661 aaaaagctgg ttcttacgag tatttattga caaagaaggc ggtatcgata tcgaagaatg
     9721 cgcctttgtc agtgagaaac tgagtgaaaa acttgatgca gcagatccag atccgattcc
     9781 tcaagcttat ttcctggagg tatcctcccc gggagcagag cggccattga aaaaagaaag
     9841 cgactatgaa aaggcacttg gtgattatgt ccatgtctcc ttataccaac cagtagatgg
     9901 cgaaaaacaa tatgaaggat ttcttcaatc atttgatgct gaacagctta cattgaaaat
     9961 acgcataaaa acacgagaaa aagaattgac ttttgaccga aaaaatattg caaaagctcg
    10021 cttagctatc caattttaat cacggagagg aatcagaaaa aatgagtaaa gaaatgttaa
    10081 atgcgttaga cgctttagaa gctgaaaaag ggatctcaaa agaaatcgtg atcgatgctt
    10141 tagaagcagc attggtttct gcatacaaac gtcactatgg ccaagcacaa aacgtcgaag
    10201 tggaatttga tcagaaaaaa ggaaagatcc atgtctatgc ggtaaaagaa gtaacggaag
    10261 aagtgatgga ttcacagttg gaagtctcat tgaaagatgc acttttaatc aatccagcat
    10321 atgagatcgg tgatacgatc cgttttgaag taacaccaaa agattttggg cggatcgctg
    10381 cgcaaacggc taaacaagtt attttacagc gtgtacgtga agcagaaaga acgattattt
    10441 ataacgaatt cagtgcttat gaaaaagaca tcatgcaagg aatcgtcgaa cgccaggata
    10501 aacgttatat ttatgtcaat cttggtaaaa ttgaggcagt tctttctaaa caagatcaaa
    10561 tgccaaatga attttatcaa ccacatgatc gaatcaaagt ttatgtttcc cgagtagaaa
    10621 atacgtctaa aggtccgcaa gtatttgtta gccgcagcca tccagaccta ttgcgccgct
    10681 tatttgagca agaagtacca gaagtctatg atggaatcgt tgaaattgtc agcatcgccc
    10741 gtgaagctgg cgatcgttca aaagtggcag ttcgctcaaa tgatccaaat atcgatgcag
    10801 tcggtacatg tgtcgggcca aagggacaac gagttcaagc aatcgtcaat gaattaaaag
    10861 gcgaaaacat ggatatcgtc gaatggaatg aagatccagc tgtattcatt gcaaatgcat
    10921 tgaatcctgc acaagtcatg gatgtgatct tcgatgagac aaatccaaag gcctgcacag
    10981 ttgtcgttcc agactaccaa ttatcgctag caatcggtaa acgcggacaa aacgcacgtt
    11041 tagctgctaa attgactaac cataaaatcg atatcaaatc agaatctgac atgacggaat
    11101 tttatgaaaa tcaagaacaa caaaaagaaa cagaagaact gcatgatgaa gcaatcgttc
    11161 agtcagattt aacagacgat gaatatgaaa caattgcatt caacaatgaa acggtcgaag
    11221 aaaaaccaga agcttaactt tagtaaggag gcaaagagat gaaacaaagg aaaattcctt
    11281 tacgcaaatc cgtggtttca ggtgaaatga aacctaaaaa agagatggtc cgaatcaccc
    11341 gttcaaaaga aggggaagta tcgatcgatc caactggaaa aatgcctgga cgaggagcgt
    11401 atgtctcact tgagccggaa gaagtacaac aagcttggga taaaaaaatt ctcgatcgag
    11461 tactcgaaac aacattaaca gatgagtttt atcaagaact tcttgactat gtcactcacc
    11521 aaaaagcacg taaagagtta tttggaaaat gaatacagtt aatcggcaaa aagccatgaa
    11581 tctcatcggt ctagcgatgc gcgcgggaaa actaatcacg ggtgaagaac tgaccattgc
    11641 tgaaatccgc aaacaaaaag caaaattggt gtttgtggca accgatgcaa gcgagaatac
    11701 aagaaaaaag ataaaagata agagttcata ttatgaagtt ccttgctttg agctattttc
    11761 tgaagcagaa atcacgcaga tgatcggaaa acctcgcaag gtattcggaa taatggatgc
    11821 cggatttgcg aagaagacca aggagctaat tgaaggttag gaaggtgatt gcatgggcaa
    11881 caaaagaatc tatgaattag caaaagaact caaaaaatct agtaaagacg tcgtggaaaa
    11941 agcacaacaa cttggcatcg atgtgaaaaa ccatatgggt gcgattacta gcaatgatga
    12001 gaagaaattg caacaagcgt tcaaaaaccc aagcacgaaa caacaagctg cacaacaagc
    12061 aagcagaaaa ccagccaatc aacagccgaa cgatcaacgt aaaaaagaaa caacgaacaa
    12121 tcaacaaaaa aataaaaata accgcagtta tcaagatcgc ggtcaaggga gcggtcaagt
    12181 gaatcaagga aaaaaaccaa caacaaacca aaataatacg ggaaatacca gcagccaaaa
    12241 tcgtcaagga acgagccaag gcggcaatca aaaccgtact aacaataaca acaaaccaaa
    12301 caataacaat cgcggcaaat ttaataataa taaccgtaat cgttataata aaaaaggtaa
    12361 aaaaggcaaa cagcaacaat caactaaacc agctgtacca ccacgcaaat tccgtgaatt
    12421 gccagatgta ttagagtata ctgaaggtat gaacgtagca gatattgcga aaaaaattca
    12481 tcgtgagcca gcagaaatca ttaaaaaatt atttatgctt ggtgtcatgg tcaatcaaaa
    12541 ccaagcactg gataaagata cgattgaact gttagcgacg gactatggca tggaaccaca
    12601 agaaaaagtc caagttgaca ttgctgatat cgataaattc ttcgaggcag aagaagtcga
    12661 tccagataaa ttagtctcac gtccgccagt cgtaacgatt atgggacacg tagaccatgg
    12721 gaaaacaact ttattggata cattacgcca ttcacgtgta acaagcggcg aagccggcgg
    12781 aatcacacag catatcggtg cctaccagat cgatattgat ggtaaaccaa tcactttctt
    12841 ggatacacca ggacatgcgg cctttacaag tatgcgtgca cgtggagcaa gtatcacaga
    12901 tatcacgatc ttagttgttg ctgcagatga tggcgtcatg ccacaaacag ttgaagcaat
    12961 caaccatgcc aaagctgcag gcgttccaat catcgtcgct gtaaataaaa ttgataaacc
    13021 aggtgcaaac cctcaacatg ttatgcaaga attaagtgag tatgagttaa ttcctgaagc
    13081 atggggtgga gaaacgatct ttgttgagat ctcagcgaaa ttcggccaaa acatcgaaga
    13141 attgctggaa atgatcctgt tagtcgcaga agtagaagat ttaaaagcag atccaacaca
    13201 acgcgcaatc ggaacagtga tcgaagcacg tcttgataaa ggtaaaggtc ctgtgtcaac
    13261 cttacttgtc caagaaggaa cacttcatgt tggtgaccca atcgttgtcg gtaatactta
    13321 cggacgtgtc cgtgtaatga caaacgatct tggccgtcgc gataaagaag caggacctgc
    13381 aacgccagta gaaatcactg gcttgaatga tgtacctcaa gcaggtgacc gtttcgtagt
    13441 cttcgaagac gaaaagacag cacgtgcagc gggtgaagaa cgcgcgaaac gtgcattatt
    13501 agaaaaccgt gctgccacaa gccgtgtgac tttggataac ttatttgaaa gcctgaaaga
    13561 aggcgaattg aaagaagtta acgtaatcat caaagcagat gtacaaggtt cagcagaagc
    13621 actggctgca tcattgaaga agattgatgt ggaaggtgtc cgtgtaaaaa tcgtccattc
    13681 agctgtcgga gcaatcaatg aaagtgatgt aaccttagca gcagccagca acgctattat
    13741 catcggtttc aacgttcgtc ctacaccgca ggcaaaagtg caagctgata ctgaagaagt
    13801 cgatattcgt cttcaccgta tcatctataa agccatcgaa gaaatcgaaa ctgcgatgaa
    13861 agggatgcta gaccctgaat ttgaagaaaa aatcaccggt caaatgatcg ttcgtgaaac
    13921 cttcaaagtt tctaaagttg gtacgattgc aggtgcctat gtaacagaag gatatattcg
    13981 tagagatagc ggtgtccgtg taatccgtga cggaatcgta atctatgaag gccaacttgc
    14041 aagtctgaaa cgattcaaag atgacgtgaa agaagtcaaa atgggttatg aatgtggtgc
    14101 aatgattgaa aaattcaatg acatcaaagt agatgacgta attgaaggat ttatcatgga
    14161 agaagtcaaa aatgactaaa aactgataaa aggaggccaa tcattatggc taactaccgt
    14221 gaccgccgag tcggtcaaga aatcttaaaa gaagtcaatg atattttgcg caaaaaggtc
    14281 cgtgaccctc gtgtggagaa cgtaacgatc acagatgtga aagttacggg ggacttacag
    14341 caagcaacga tctattacag cattttatct gacttagcat cagataagaa aacagcacaa
    14401 gatggattga ataaagcatc aggattgatt cgtcgagagt tagggcacaa tctaagtatt
    14461 tataagacac ctgaattgtt ctttgaactt gatgaatcag ttgtctatgg caaccatatc
    14521 gatgaattgc ttcgcgactt gaacaaagac taatcaaaaa ctcccgcaaa tcctgtgaaa
    14581 tcaggggttg cgggattttt ttgttttcat taagacgtat tacctaagag ttccatgact
    14641 ttagtcttgc ggcgtgtcag ctaaccaaaa aagagaaaaa gaaaagtaaa aaattcgact
    14701 ttcttttttc tagtagtata taaatttttg catatagata aagatcaaga taccgataac
    14761 ggaagctaga ttgtaatcta ttttagtgac agtcaggtat agccaagtaa ctagaagatc
    14821 agtggaacct gcaccataaa agtttgagtt tgtcggtccg ttgacagtta aaagaaaaat
    14881 cacgttgaag ttattgatat ttccgattac gccgcttcga tttggtcacg atccaaattt
    14941 tggataatcc ctgtagaaat tagcatagta gctgggatac caattcacat attgacaaag
    15001 ataacagaga cttttacata aaacggatca atcaggaaag gaattgctcg atccagcccc
    15061 agttttccag caatgtgtta actggcccag cgtcattcag caaattatgc atgatgagga
    15121 gcgaaatgaa ctgcggaaca gccattgtga taacgacaag ttttttggat caatgtaaaa
    15181 aacgtcagcg aaaggaaaaa tttcgtggtt ttttatacct gttcatttgg ctagatataa
    15241 aatccaaaca aatgaacaga agtgcttatt aggtttcaat tgggagtatc tgcgctagaa
    15301 taagagtaat aatgaaaagg agtgttatgt atggattatt ctagtaaaga atactttgat
    15361 aaaatgacgg cttggtggcg tgctgctaat tatctttctg ttgggcaact ttatcttaaa
    15421 gataacccct tactgagacg tacactgaaa cccgaagatg tcaaaaaaca tccaatcggt
    15481 cattggggaa cgattcctgg ccaaaatttt atttatgtcc atttgaaccg agtcatcaat
    15541 aaatatgatt tgaacatgtt ttatattgaa gggccaggac acggcggaca agtcatggta
    15601 tcgaatgctt atttagatgg aagttacaca gagatctatc cagaagtaac ggaagatgag
    15661 acaggaatgc aaaaattatt caaacgattt tcatttcctg gaggcattgc ttctcatgca
    15721 gcaccagaaa caccaggttc tatccatgaa ggcggagagc ttggatactc tctttcacat
    15781 gcagtcggag ctgtattaga caatcctgaa gtaatctctg ctgtcgtgat tggtgatggt
    15841 gaagcagaaa ccggtccttt agctggaagc tggttctcaa atgtctttat caatccagtg
    15901 atagatggag ctgttttacc gatccttcat ttaaatggtg caaaaatagc caacccaacg
    15961 atcttggcac gtaaatcaga tggagaatta gcaaattact ttaacggact tggctgggaa
    16021 cctttcttta ttgaaggaaa tgattcagaa aaattaaatc ctgttatggc tgaaaaaatg
    16081 gatcaagcaa tcgagaaaat taaatccatc caaaaagaag ctcgcctgaa gacagcaaca
    16141 gatgtcgtga tgccaaaatg gccagtattg atcgtacgca caccaaaagg atggaccggt
    16201 ccaaaagaat gggacgggga accaattgaa ggaaccttcc gagcacacca agtaccgatt
    16261 cctgtagatc aagaacatat ggatcatgca gatgctttac tccgttggct gaaatcctat
    16321 gaaccagaaa aattatttga tgcacaagga cgtattttag aagaaatcag agaaatcgcg
    16381 cctactggtg accaaagaat ggcaaaaaat cctatcacca atggtggaat cgatccaaga
    16441 ccgcttatta tgccagattg gaaaaaatat actttgcaat ttgaaaaacc aggctccgtt
    16501 aaagcagaag atatgaccga acttggtaaa ttcgttcgcg agatcattga gaaaaatcca
    16561 gaaaatttcc gtatatttgg accagatgaa acaaaatcaa accgattgaa tcaagttttc
    16621 aaaacgacaa atcgccaatg gatggaaaaa atcgaaccag aaaatgatga atggctttcg
    16681 ccatctggtc gcgtcatcga cagtcaatta tcggaacacc aagatgaagg gttcttagaa
    16741 gggtacgttc tgactggacg gcatggtttt ttgcaagtta cgaatcattc ttgcgagtgg
    16801 ttgactctat gttgactcag cacttcaaat ggatgcggaa atcacatgac ctttcttggc
    16861 gaaatgatta tccttcgcta aatctcattg caagctcaac agtcttccag caagatcaca
    16921 atggatatag ccaccaagat ccaggaatat tgacacattt ggcagaaaag aaggcggagt
    16981 tcatccgtga atatctgcct gcggatgcaa atacattgct cgcagtcatg gataaagcat
    17041 tccgctcgtc agaaaaaatc aatttgatca tctcttccaa acatccgcgt gctcaatttt
    17101 actcagcaga agaagcagcc gtcttagtga atgaaggctt gaaaatcatt gattgggctt
    17161 cgacagcaaa agaggaggaa cctgaattgg tcattgctgc tgcaggaacg gaatctaact
    17221 tagaagcatt agcagctgta actttgctgc tggaagaatt cccaaaacta aaaattcggt
    17281 tcatcaatgt cgtagattta ttgaaattac gtcatccaag tcaagatccg cgtgggttga
    17341 gcgatgaaga atttgatcaa tattttacaa aagacaaacc gatcttattt gctttccacg
    17401 gttatgaaac attggtccgc acgattttct ttgatcgtca taaccatcat ttaatgattc
    17461 atggctacaa agaaaatgga gatatcacga caccatttga catgcgtgta gtaaatgaac
    17521 ttgatcgtta tcatttggca aaagacgcag ctttaaaaat caaaggaagt caagcagaag
    17581 attttgcgaa aaaaatggat cagaaattac aagaacatca aaactatatt cgtgaaaatg
    17641 gtattgatct gccagaagta ttggattgga aatggaaaaa tcttgaccaa taaaatgata
    17701 aaatcgatcc cgcaaaatag aattttttac attgattaag aaactgaaaa aataaataaa
    17761 aaaaacggaa aaaagactgg aagaatggcc agtctttttt ttcgtttttg ggaagtgaaa
    17821 accaaactat ttacctgtaa gccacccata aatccaatca aatggtgcaa agaaaaaatc
    17881 ttccattcta ttccctcctt taaaaaagca taacatgatt tctatttacg tgagcatgaa
    17941 ttttgttacg tttttattgc cgaaaaataa aaaccgttca ttatgatgtc aaattcgtca
    18001 gtaattgatg caatgtgtta taataattca gttaaatcaa aaatgaaatg gagaaaagac
    18061 atggacgggt tattaccttt atggaaagaa cgcggaatga caagtcatga ttgcgtattc
    18121 aaattaagaa aaatcttaca tacgaaaaaa atcggacatg ggggtacgct tgacccagac
    18181 gtagacggtg tgttgcccat ttgtattgga aaagccacaa aagttatcga atatttgaca
    18241 gattctggga aaatctatgt aggcgagata actctaggtt tcagtacaac gacggaagat
    18301 cgatccggag aacgggtaga aacaaaacca gtagatatgt ctttatctga aaaaaaaata
    18361 gatgaagtga tggcttcatt tattggtgag atcacccaga taccaccaat gtactcagcg
    18421 gtaaaagtca acggtaagcg gttatatgaa tatgcccgca ataatgaaac agtggagcgt
    18481 cctgttagaa aggctcagat ttaccgtttt atccggacaa gtgaattgac aagagacgaa
    18541 gtgacaggaa ccatttcttg gcgcttcgaa gtagaatgcg ggaaaggaac ctatgtccgc
    18601 acattagctg tagatacagg caagaaactt ggttaccctg cgcacatgtc tgatctgacc
    18661 cgaacagcaa gtgccggtat gtcccagaag gatgctctga cattagaaga agtagctcaa
    18721 gctgtgaaaa atgggacaat cgatgattgt ttgctgccaa tagaaaaagg agtggaacgg
    18781 ttcaaacgag tagatattac tgatgaagta tggcagaaag taaaaaacgg tatgagactt
    18841 gattatactg tatttgcgct cccagaaatg ccgtcagaag aaattgcttt gttttataaa
    18901 ggaaaagttg tcagtattta tcagccaaac ccgaaagaaa aaaacaaatt aaaacctagt
    18961 aaagtattga gaaatgagga ataacgaatg aatatcatca agatcagaca tccttaccaa
    19021 tcagaacaaa ttccagaaga cgatgtcgtt ttagtgctgg gatttttcga cggcgtgcat
    19081 atcggacacc agaaagtcat aaagacaggt atggaaattg cacaaaaaga aggattgaaa
    19141 ttagcattga tgactttcaa tcagcatcca tctattgtct ttaaaaagat cgatcctagc
    19201 agcgtgaaat atttgactag ccttaagcaa aaagaagaaa aaatggccgc tttgggtata
    19261 gattatttat atgaagttga ttttacttct tcttttgctc atttagcacc gcaggaattt
    19321 gttgatcaat acatcgtagg gttacatgca aaatatgctg tttccggttt tgattatacg
    19381 tatggaccaa aagacattgc agatgtcgct cattttcctg aatatgccaa aggaagattc
    19441 aaaatcgtca cagtttctaa agaagaagaa caaggtgaaa aaatcagctc tacccgtatt
    19501 cgtggattac tagacagcgg acaagtaaaa gaagcagcaa aactattagg ttgcacatat
    19561 gaggtagaag gggtagttgt ccatggcgat gcacgaggca gactactagg attccctaca
    19621 gcaaatgtca aagtaaagag cacggttcat ttgcctaaag aaggtgttta tgtgtcggaa
    19681 ataaaaattg gggaaacatg gtatccagcc atgggttcaa ttggacataa tgatacattc
    19741 ggtgaaggac gggagctgac tgtagagttg tatattttgg actttaatca agatatctat
    19801 ggtgaacatg tcgcggtacg ctggaatcaa tttttaagag atcaagtgaa attttctagt
    19861 gtagaggaat tgattgaaca actcaaggag gatgaacaag caacagctga atatttttct
    19921 cagcaataaa acattatgca taaaatctct tgtttatgta taactagcaa ttacaataaa
    19981 atgggtgata aataagccca ggaaaggggc tgtgacaaaa gtcttgtcac agcctttttt
    20041 tccaaataaa tggtttttca gaagtagctt cttcggaaat aagccgaaat ttcttgaagc
    20101 tgaccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag caattcatga
    20161 aaacgattgc tagagcgtat catttgcttt agactatata tcttggtgta cactcacttt
    20221 aaggcggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg agtgggtact
    20281 atgtacaagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa ggggattgga
    20341 acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa ctataactcg
    20401 gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg aaaagcagtc
    20461 tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga tacggcttta
    20521 gaaaattttg ggacactgga cgtttgggtt aataatgctg ggatggaaaa tcagcatcca
    20581 acccatgaac ttagccttga agattgggaa agagtgttaa aagtcaattt gactggcgtt
    20641 tttatcggaa cgaaagcggc tttaaattac tttacagaga ataataaaaa aggaaatatc
    20701 atcaatatgt cttctgttca tgaacagatc ccatggccaa cgtttgctca ttacgctgct
    20761 tcaaaaggcg gggtaaaatt atttaccgaa acggtagcaa tggagtatgc accgaaggga
    20821 atcagaatca atagtatagg ccctggtgct atccgtacac ctattaatgc ggcaaaattt
    20881 gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg cgtaggtgaa
    20941 ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag ttatgttaca
    21001 ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca aggtggaaaa
    21061 ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt tggctggggg
    21121 atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat attaggaact
    21181 ggtattggag cggtgcttgt cggcttactc gttcaattat tcgcttctcc agctggtatt
    21241 tcagtgacta catttttgat tagtttactt tctggtgcat tttgggttat tggacaaaca
    21301 ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat ttcgactgca
    21361 ttacagttag tcggaacctc gttgattggt gtcttcgctt ttggagaatg gtctggaagt
    21421 atggggaaaa tcattggagc agcagcaatc attcttcttg tatttggttc tgctttaacg
    21481 gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaattag tctattagca
    21541 tcaacaagta ttggttattg ggtatacagt gcattgccta aactagttga tgcaagcgga
    21601 ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat ttatgtgatt
    21661 ttccgtaaac caaccgcatt ttctgaagac aagagttgga aagcaacgat cgttggagcc
    21721 atctttagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg ggttgctact
    21781 gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat gtacatcctg
    21841 catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt gctgatcgta
    21901 gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaattt catttattaa
    21961 aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct agaatttcca
    22021 acatctctgg aatttctagc gctttttaat tgtttaagat acaagggaaa tatctgtctg
    22081 gatcaataat ttttcattga tccattgact aaagtttacg acataattga agttaccacc
    22141 gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac ggtctttttt
    22201 atttgcgtaa acatcgttat ggtactggac taattgttct cggaattgat tgattgctgt
    22261 aaaatcacga tattgaaggt tgaaacgcat cgtgattggt gcatttagca tgaatcgctg
    22321 catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa gattacgttc
    22381 agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat gaagataatt
    22441 tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg agtaaaaatc
    22501 tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat ttgcttaatc
    22561 acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt cagaaaattc
    22621 taacttttat ttctctaaag aaattcgttc tctcctaaag tgatattata tttaaatata
    22681 cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat tttttcctta
    22741 ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actatgtaga aaattcaaag
    22801 ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat agcagatact
    22861 ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca aaacctgtat
    22921 aaggtgatga atagtttcct cgccagtaaa aggtttattc gtataaagct ttatcgccaa
    22981 aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc aatcaattcc
    23041 cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac gtagcctgct
    23101 tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat agaaaaatga
    23161 tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat aggttggttc
    23221 cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa aaggtctttg
    23281 atcttttgtt gatgttcttt ttctttgatt tgattgatga tcggctgaaa tttttccatt
    23341 ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata gttatgtata
    23401 aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata agatgtctca
    23461 aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat tcttacacga
    23521 aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag atgctttggt
    23581 aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct taaaagataa
    23641 aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt tcaaagcatt
    23701 agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg aagaacaaac
    23761 gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc cgattattat
    23821 tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac cagatcaaac
    23881 gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga tgccgatatt
    23941 tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata cttggaggtg
    24001 aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg ttactgcacc
    24061 atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa agagaatgga
    24121 agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata aggaaaaatc
    24181 aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg aaaatatcca
    24241 aatgatcttt ccgacttggg gaggagaatt attgattgag attctcgatt ttgtcgattt
    24301 tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt tgctacttgc
    24361 tgtcacatta aaaacaggga tagccactgc acatggaact aaccttattg atttaagagg
    24421 agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa gagaaggaga
    24481 atcgattcta cagcaatctt ctgaaaagta tcaaaaagtg tggccccatg agcattcatc
    24541 atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg gagatgcagt
    24601 aaggatgaag ggacgattat taggtggttg tattgatacg atcaggcatt taatcggtac
    24661 tccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc cgatcttgtg
    24721 gtttctagaa aactgtgagc tatcgtcagt tgatttaaga agatcacttg ttcagatgaa
    24781 actggcaggc tggtttgatg gcagctcggg gatcatgttc ggtagaagtg cggcagagga
    24841 tttgttagat tataaaatga aggatgtata cgaagatctg tcaaaggaat taaaactacc
    24901 gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga ttaatggtgc
    24961 ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt ttatttaaaa
    25021 aattttttcc tgccttggag tctatcaaag accaataaat ctaaatagaa aagaaagtga
    25081 aagaagctga caaaatgctg tcagcttctt tttgtataac atattagaaa gtctaattat
    25141 ctataactgt cacatcgatt aactgtttca cgtcattggg atttcctgta ggaatagcta
    25201 ctacagatgc attatcaatg atttcttgag ttgtttctct aatgaacgct ggataagatg
    25261 tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt gtcccgtcat
    25321 ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc ataaccacat
    25381 ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct gcaggataat
    25441 gaataacatg taattcgaaa tcacccgatg tatctctaaa agtattacct ctaatagcta
    25501 gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga tctattgact
    25561 gagaagactg tgaggaggtt gtgtctattg ttgaagaaga aatatttgtc tcaactgtac
    25621 tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa gatgtattcg
    25681 tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt tgtctgattg
    25741 aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac gtacatccta
    25801 caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa aatacctcat
    25861 tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa accttgtcta
    25921 tgaatataaa tagagtgtat cttctttgat aaatgtcaga aaaagtgtga caaataatca
    25981 tcagtaaaat tacttgtcac actattgata aggagtattt aattgaagag aatgattagg
    26041 agaaaggatc tccgtacttt ttatagtaat catagaatcc aagtttgtat agacgaatcg
    26101 ttttttctct tatttcttct gtttcaaaat ggactgcttg aagaatcaag tctgtgaatt
    26161 ctataggagc tgttccattc gcagtaatca aattacgatc tttcacagct tgttgttcta
    26221 taaaatcttc tttattctga taattttccg attctaacca aggaaattgc gcatttccgg
    26281 tgtgtttgta accttgcaat aatccgctct gagctaaata atcaactgcc ccacaaatag
    26341 cagctactgg tacgtctgtt tttaacaatt tatcgattat ttgatataaa tgatgatttt
    26401 ttatgtccca tgaattgcca ccgatcataa taaaaaggtc aactttttca ggtatttgtt
    26461 cgattaagta atcgattttt gtttttaatc ctccaatgga agtgacctct gattggatag
    26521 aagcagtttt gttttgccat ttctcatttt ggtttagtgt gctagtaata taagcacctt
    26581 cccaatcggc atattcctct aatagaaacg taatcgcagt tttcattttc aagctctcct
    26641 ttactatcta taacaaggac aacagtttta acctacagac attcattaaa attgggtagc
    26701 gacagcgaaa acaacaattc ttaagtttta gacagagtat acagatagtc aacttaatta
    26761 aatacgaact aaataaaaat gacaggtaat catttttgtt taccagcatt ataacatttt
    26821 gcatacatgg atgacaaaag aaccaattcc cacttgcttg agtacaatat taaaagaaac
    26881 tggaaggcaa aaagtaatta tagaaacgaa aaataaatca tatcgggcac tcagacatga
    26941 aaacaaccct agagatatat aactaggtaa ccagtttaat gaaagaaaaa atcatccaag
    27001 aagtcgattc ttggattttt tagtggattt gttcgccaaa tacgtttagt atgcgtatat
    27061 gagaggttgt aagcaaagcg ttgtggtctg agcacttaag gaggaatctg tgaaaatagc
    27121 ttttcatatt tttgcagatt ttgacttaat gccgaaggtc agctttgcga ttaccgttta
    27181 tcgagaggct gtaaaagcac cgtgtagctc cgatgacaat aagcaagcat ttttagagct
    27241 aggtttcctt gattctaaat atgcggctta tttcaatgag ttggtgcttg aacgcgattt
    27301 taagaaggtc gaaaacaggc acgacttttc gacgtaagga gaaagaattt caatgataga
    27361 aacaataaat cccgcagaaa acaaggtttc tgcgtacatt catatcccat tttgcgagca
    27421 tatctgctat tactgtgatt ttaataaagt ttttttagaa gggcagccag tagatgagta
    27481 tgtggaaatg ctgctgaatg aaatgaaaat aatgctagaa agtcatccga cagcgaattt
    27541 agaaacactt tacgtagggg gcgggacacc aacttcttta tctgctaaac aacttgatcg
    27601 actgttatca ggtgctagag agatcctgcc ttttcaaaat ggtaatgagt ttactgtaga
    27661 agcgaatcca ggtgatttga cccgtgaaaa acttcaagta atgaaaaact atggcgtcaa
    27721 tcgtttatcc atgggagtgc agacatttga taatcgctta ttgaaaaaaa ttggacgtaa
    27781 acacaccgca gaagatgtgt atcaaactat gcaattttta gaagcagaaa attttaccaa
    27841 tgtaagcatc gatttgattt atgcattacc gggacaaaca ctagaaggat atctggatac
    27901 tttggaaaaa gctttggctt tggatctgcc tcactattct ttgtactcat tgatcctcga
    27961 aaacaaaaca atgttcatga actgggtacg acaaggaaga ctcgaactgc ctgatcagga
    28021 aacggaaacc agaatgtttg aagagacgat tgaagcaatg gaaaagcatg gacgtcacca
    28081 atatgaaata agtaactttg ggctctccgg acatgaatct caacataacc tcatgtactg
    28141 gaacaatgac cactattttg ggtttggagc tggtgctagc ggttatcttg gagatattcg
    28201 ttaccgcaac aaaggaccaa tccaacacta tttacgccca ttacgagcaa atgaacgccc
    28261 ggtattggaa gaagaaaaat tgacacgaaa aaatcaaata gaagaagaaa tgttcttagg
    28321 actaagaaaa aaaacaggga ttactaaaaa acatttctat gatcgctatc aaaaaacaat
    28381 tgaatcggtg tacggtgatg tgctaaggga attagagcaa gaagggttac ttgttaatga
    28441 agaagaccgt gtttatttga caccaagagg gacctttttg gggaacaatg tgtttgagcg
    28501 ctttttgttt gaaaaatagt ttaaagaata tttgtttttt atctgaattt aaataaaaaa
    28561 ag
//
LOCUS       DAZUKZ010000026        27784 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000026 DAZUKZ010000000
VERSION     DAZUKZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.026"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(253..1197)
                     /locus_tag="ACG8EF_001091"
     CDS             complement(253..1197)
                     /locus_tag="ACG8EF_001091"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS4946429.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1493..2437)
                     /locus_tag="ACG8EF_001092"
     CDS             complement(1493..2437)
                     /locus_tag="ACG8EF_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4946430.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2657..3871
                     /locus_tag="ACG8EF_001093"
     CDS             2657..3871
                     /locus_tag="ACG8EF_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4946431.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3893..5908
                     /locus_tag="ACG8EF_001094"
     CDS             3893..5908
                     /locus_tag="ACG8EF_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFS4946432.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5991..7364)
                     /locus_tag="ACG8EF_001095"
     CDS             complement(5991..7364)
                     /locus_tag="ACG8EF_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS4946433.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7390..7674)
                     /locus_tag="ACG8EF_001096"
     CDS             complement(7390..7674)
                     /locus_tag="ACG8EF_001096"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4946434.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7676..9691)
                     /locus_tag="ACG8EF_001097"
     CDS             complement(7676..9691)
                     /locus_tag="ACG8EF_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4946435.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9870..10175)
                     /locus_tag="ACG8EF_001098"
     CDS             complement(9870..10175)
                     /locus_tag="ACG8EF_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFS4946436.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10264..11295)
                     /locus_tag="ACG8EF_001099"
     CDS             complement(10264..11295)
                     /locus_tag="ACG8EF_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4946437.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11502..12377
                     /locus_tag="ACG8EF_001100"
     CDS             11502..12377
                     /locus_tag="ACG8EF_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4946438.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12529..13371)
                     /locus_tag="ACG8EF_001101"
     CDS             complement(12529..13371)
                     /locus_tag="ACG8EF_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4946439.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13463..15340)
                     /locus_tag="ACG8EF_001102"
     CDS             complement(13463..15340)
                     /locus_tag="ACG8EF_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4946440.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15337..16758)
                     /locus_tag="ACG8EF_001103"
     CDS             complement(15337..16758)
                     /locus_tag="ACG8EF_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4946441.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16761..20150)
                     /locus_tag="ACG8EF_001104"
     CDS             complement(16761..20150)
                     /locus_tag="ACG8EF_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4946442.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20407..21798
                     /locus_tag="ACG8EF_001105"
     CDS             20407..21798
                     /locus_tag="ACG8EF_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4946443.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21871..22518)
                     /locus_tag="ACG8EF_001106"
     CDS             complement(21871..22518)
                     /locus_tag="ACG8EF_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4946444.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22526..23212)
                     /locus_tag="ACG8EF_001107"
     CDS             complement(22526..23212)
                     /locus_tag="ACG8EF_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4946445.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23218..24291)
                     /locus_tag="ACG8EF_001108"
     CDS             complement(23218..24291)
                     /locus_tag="ACG8EF_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4946446.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24513..25187
                     /locus_tag="ACG8EF_001109"
     CDS             24513..25187
                     /locus_tag="ACG8EF_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4946447.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25192..26532
                     /locus_tag="ACG8EF_001110"
     CDS             25192..26532
                     /locus_tag="ACG8EF_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS4946448.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26681..27667)
                     /locus_tag="ACG8EF_001111"
     CDS             complement(26681..27667)
                     /locus_tag="ACG8EF_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFS4946449.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8098 a         5248 c         5045 g         9393 t
ORIGIN      
        1 tagatatgaa tcattgtatt tgccgttgcg gaaggatttt ttaattaacc acagataaac
       61 attgataacg attcgcttat tgtaatcaac agattaaatt tcatatgttc atatgtacgg
      121 atacattttc ggaccgatta attttaacca ttctgtttat ttgcttacct attagatatc
      181 aataaaaaac tgcttaacag ctatttcagc tgttaagcag ttttaggatt tttagttcat
      241 attcattgtt tcttataata cagtgttcaa tacttctctt aaagcacttg ttgatttgct
      301 gaacattgct ttttcttctt cgttgattga taattcaaca acttcacgaa caccattttc
      361 gtcaacgatt gatggcacac ctgtaaagat atctttttcg tcgtattcac cagttaaata
      421 agcagataca ggcaatacag cttgttcgtt attcaagata gcttttacga tacgtgcagt
      481 actcatacca ataccatagt aagttgcttt tttacgatca atgatctcat aagcagcatt
      541 acggacttta tcagcaatca catctaattc atcttgggca atacgatggt ctttttctac
      601 aatttcaaac actggtttac caccgacagt tgtatgtgac catgcagcaa cttctgaatc
      661 accatgttca cctaagatat agccgtggac gctgcgtggg tcaactttta atttcaatgc
      721 gatttcttta cggaaacgag ttgtatccaa tgttgttcca gtaccgataa cacgtgatgt
      781 tggcagacca gattcgttcc aagcgatata tgtcaaaata tctactgggt ttgaagcaac
      841 aacgataata ccgtcgaatc ctgatcccat gatttctttt acgatttggc gcatgattga
      901 cgcatttgtt ttaactaaat ctaaacgaga ttgaccaggt ttttggttga taccagctgt
      961 gataacaacg atatcagcat ctttacattc ttcatagcca cctgaccata cagccacatt
     1021 ttcatcgccc caagccatac catctaaaag gtccaatgct tccccttctg ctttttcttg
     1081 attcacatcg atcaatacta attcattaga gattccttgg ttgatcattg cataagcgat
     1141 acttgttcca acaaaacctg taccgacgat tactacttta cgacttgttt ttttcatgaa
     1201 taaacagctc cttttcataa ctatttgtta ctttcatgaa aagtatagtc ttgtttattc
     1261 attttatcaa catgttaatt ataatagttg ttattataat ttttgatgga aacgaattcc
     1321 tgaatacgca taacagataa atgacaaatg tttacaccta tatcccctga taatacaacc
     1381 aaaagtttag aactgaaaat aaatccaaaa aaattcgctg caacgatata ataagacagc
     1441 aatcgcacct aaaacaattt tccttcatat gctttagtga aagattttct tcttaatgtt
     1501 tatttctcca ttcattggga gtacatgttg aatacttctt gaataatttg aataaagaag
     1561 tcgtttgttt atatccgata cgattagcga tttcttccaa cgaaaaactc gtttcttgaa
     1621 tcagttccaa cgcaaagtcg aattttttct ttagaaccag ttcttgaaag gaagcatcaa
     1681 aatacatttt caatttatta ctaagataga ccggattgta gccaaaatga gctgctgttt
     1741 cttttaacgt acaagaagta aaatgcttat cgatataatc taatactcta agcatctcat
     1801 tttctgcgca tccatctgct gatgaaaagc tttgttgttc taataaatga gtcaattcga
     1861 tcagtaatag tgatgtatag agatttaacg aacggatctt atgactgtat gtatcaaaat
     1921 aaatcaacag catattttca atcaaatgcg tcaaacgact tgtttttccg ccgcgaaaat
     1981 gaatgaattg tgtatgttcc cctttttctt ttgatgcatt tatcaaaaaa gaagaaagta
     2041 gattatttgt gcttgccaaa tgattcaata tctctgtaga taaggtttct tcttttaata
     2101 agatgttgat taaaatatcc tcttgccctg tgtattcgat tctttgagta gcttctttat
     2161 caatgatcac cacgtcactt tcttccatct ctatcctttc tccattaata atttgtaaac
     2221 atgtaccgct atacatataa ttgatttcaa caaaatcatg tgtatgctcg ggaacttccg
     2281 catatcgacg gtgtttactg atatgaatat ttccctgttg aaatagatcc cctcggtgga
     2341 tttttggcgg tgtctgaaga tggtaagcta agtttaaatc attgatattc attccttgtt
     2401 cacgctgcag agtttcgatt gattcttcgt tacgcaaaaa aatgtctaat tcagctagtt
     2461 ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca cgctgtactg
     2521 ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact
     2581 tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga taagcgctta
     2641 caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt
     2701 ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac
     2761 ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct cttttatggt
     2821 cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt
     2881 gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct cacaaagtta
     2941 cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt
     3001 ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt
     3061 tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag gacagctagt
     3121 acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta ttcttgtctt
     3181 gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg gcacagcccc
     3241 aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct
     3301 ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc
     3361 tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc
     3421 tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc
     3481 tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat
     3541 tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa
     3601 ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa tcttattggt
     3661 tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct cttttgcttc
     3721 tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt tgattagcgc
     3781 gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa gagtagatga
     3841 agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta aaatgacaaa
     3901 tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc gccatggact
     3961 gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact ggtacatgga
     4021 tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac cttccatgtt
     4081 taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac atccatgtgg
     4141 aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag gacatacatc
     4201 agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg gctggacagc
     4261 aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg atatcgcaaa
     4321 agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg aaacggagat
     4381 tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag aactaatatc
     4441 tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg tagaagctat
     4501 ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga aagctcgtaa
     4561 acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc aacttcaagt
     4621 ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt cttctttact
     4681 gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt tgttttttat
     4741 cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat cattcatgat
     4801 caatcaatat cttttttttg acgaggaaat caataaacga acgatattag cattaagagg
     4861 acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct tgtggttgat
     4921 tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca tcgtctaccc
     4981 taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag ggtttattta
     5041 tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag aaggaacgct
     5101 tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt gcggagatat
     5161 cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc aagaaaaata
     5221 tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg gcgcttttat
     5281 cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt
     5341 attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct tattgaatga
     5401 cactatcaca cagattacta ctccatattt caaattctat gaacaagatg ctttatgcaa
     5461 actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag ggatgttatc
     5521 tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa atgaaaagta
     5581 cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag caagcccaat
     5641 ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt atgaagaatt
     5701 tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac ctatcaaaga
     5761 tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg atagaacagg
     5821 cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt
     5881 aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat atcagaagac
     5941 tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc ttaattctgt
     6001 tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa tacgacaagt
     6061 aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc tagtttccca
     6121 agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc gattttaaga
     6181 tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct attgaaagca
     6241 gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc agaagcagct
     6301 ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa gcctatcata
     6361 gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa tacagcatta
     6421 ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg tacgagtttt
     6481 ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac cccttgaagg
     6541 acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat tacagcgttg
     6601 tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca
     6661 tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa gctgaaactt
     6721 ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt tccgatccat
     6781 ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt gtttccagtg
     6841 atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt tccttcgaag
     6901 accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac taaaatgact
     6961 gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa tttatatctt
     7021 gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa agcagtaggt
     7081 gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac tactccttga
     7141 gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact gattaaacca
     7201 gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg gatcacttct
     7261 gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac gaggatggag
     7321 ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag ttcctccctt
     7381 gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt cttcgctgtc
     7441 aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg tatctttcaa
     7501 agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa catccgtatg
     7561 acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt tgatgttcat
     7621 ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt tcatatcatt
     7681 cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt aataaatctt
     7741 gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga agatgtgcct
     7801 cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga ttatcaagca
     7861 gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg ccatcttctg
     7921 gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca tactgctcca
     7981 cgcgctcgat catagctttg atataacttt cttctatttt attttctgat aatagcggct
     8041 gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat tggatcattt
     8101 cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct aaaagtttgt
     8161 tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct tttaattcga
     8221 tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat cctggtatca
     8281 gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc atgattggat
     8341 gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta gaaaaaataa
     8401 tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa
     8461 agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag ctaatgccac
     8521 ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc gcttttctga
     8581 tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg ctcttcccta
     8641 ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa gcatattgat
     8701 gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata cgaaaatagg
     8761 cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca aattctacgg
     8821 ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg
     8881 caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa aatataatct
     8941 ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta atgtcagtga
     9001 ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt tttccaattc
     9061 gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt gcgaatcgaa
     9121 gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa taatcaatgg
     9181 aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc atcaaacgag
     9241 cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc ctaaaaccag
     9301 ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt atctttttga
     9361 tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa
     9421 cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct tgttcggtat
     9481 acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt gctcggaatt
     9541 tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat tgtttgatcg
     9601 cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt tctcttggtg
     9661 ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac ttatattcta
     9721 gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg atttttttta
     9781 cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc
     9841 tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt ttttgaggtc
     9901 agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt ttcaaagtga
     9961 ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa agctgtttct
    10021 ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc ttctgtttca
    10081 ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat tgaacctctt
    10141 aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag tagttctata
    10201 ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat tcttacaaat
    10261 atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca aaattttcat
    10321 ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta atgtctcgta
    10381 aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt cttcatgata
    10441 actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat cgataccaaa
    10501 gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat cttttgtttg
    10561 tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa
    10621 ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat cgaagtaatc
    10681 attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat ctggttcgcc
    10741 gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat agctgttcgc
    10801 agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat aatgcatttc
    10861 tacttctaaa atatccccta attttccgct ttcgattact ttttggactg tcaagaagtc
    10921 agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt ttgctaatgc
    10981 aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta gtacattctt
    11041 accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg tagtgatgac
    11101 aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat attcgatgtc
    11161 ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat tgattttgtt
    11221 cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat tgccaagata
    11281 agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata tcctagaaaa
    11341 gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac acttgttatc
    11401 attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga
    11461 accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt gaagaaaaac
    11521 taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac atccgtagga
    11581 atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg tatacttccc
    11641 attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt
    11701 tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg attgatccta
    11761 attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg gcggatttat
    11821 cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta gatcaaatcg
    11881 gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat tctcaaatca
    11941 cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc atggctgaag
    12001 aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc gccattttta
    12061 ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg aagcatgtag
    12121 aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa caggctgata
    12181 tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact ttctcctctc
    12241 aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct caaaattttg
    12301 aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag
    12361 aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag tgtttgatag
    12421 gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt
    12481 ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct aacttctgtt
    12541 tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca gtatacatgc
    12601 agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta cttctttttg
    12661 aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat gactgttgac
    12721 catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga ttctcagatc
    12781 ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc
    12841 attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg tgaacaactt
    12901 tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc cccccgtagg
    12961 ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat tcgtttcatc
    13021 aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc
    13081 aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat ctgcacctgg
    13141 atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt ggattttagc
    13201 catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga tctgctgatc
    13261 cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta ttcctgagag
    13321 aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca tttgtgttct
    13381 ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct
    13441 ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca aagtacagtc
    13501 cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc ttaccgcctg
    13561 ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc aactgtcctg
    13621 ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat ggaacatatt
    13681 tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct gtcacatcga
    13741 ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat acccagctga
    13801 cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct tgatcacgaa
    13861 cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct actttaacaa
    13921 atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt gtatgcccgt
    13981 tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg ttcaaatgca
    14041 tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa ttcggatcaa
    14101 ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg tcgatttctt
    14161 tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca aatgttaaag
    14221 cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt ccttctttat
    14281 cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca ccgattgcga
    14341 catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca tttttgatgg
    14401 tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt gtttgctttt
    14461 caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt ccaacttcta
    14521 aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca ttgttgccga
    14581 tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt gtccaagtgt
    14641 acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct tcttctttag
    14701 aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt tttgtaactt
    14761 tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta tctagttctt
    14821 ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca gggaaagcta
    14881 aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc gtgtagactg
    14941 catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca gcatctgtcg
    15001 ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt gctgcatcca
    15061 cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa actgaaaatg
    15121 aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg ctgttttgga
    15181 ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga gctgctgtct
    15241 cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa
    15301 ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc ctgttctttt
    15361 tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc ctaaaagtgt
    15421 atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt tattgactac
    15481 tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg taaacggaat
    15541 ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc gataacttcc
    15601 tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc
    15661 tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt
    15721 gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg tctcgatttt
    15781 gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac caaaaccaaa
    15841 atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc ttagctgcat
    15901 ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga tcgaaaataa
    15961 aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct tttctccatt
    16021 cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac tatctgacaa
    16081 ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg aataagtgat
    16141 tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa aaggtggatc
    16201 agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt tttgattgtt
    16261 ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga cgacttctgg
    16321 tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt tagcggccag
    16381 agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa
    16441 cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta ccgttttgcc
    16501 cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg tgacaccatt
    16561 taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat gatttttcgg
    16621 cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt
    16681 ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac acatactaag
    16741 ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa aagataccaa
    16801 atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc aattcctccc
    16861 gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg gttgtttttg
    16921 gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga cccatcttcg
    16981 tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt aaaagtttcg
    17041 gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca
    17101 tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc tccttttaat
    17161 gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag tcgattttga
    17221 tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta aatgattttt
    17281 ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc ggcaggacca
    17341 gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc aagacctttt
    17401 tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt tttctgtagg
    17461 gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc atacttacgt
    17521 actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt atcatcggaa
    17581 gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt gatttcccaa
    17641 gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag cgtataggtc
    17701 atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc atgatctgtt
    17761 agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag atccttttca
    17821 cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt agtatttttc
    17881 catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc tttgattgtt
    17941 tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat ctcttgataa
    18001 ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt atttgttgta
    18061 tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc atttgtcgta
    18121 tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt gttgtcaaat
    18181 tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga tggtataccg
    18241 aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc gttcatttga
    18301 taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg gtaatggatt
    18361 tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt tccttctatg
    18421 gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga aagtgtacca
    18481 ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct tacagttgct
    18541 gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg atcagctgat
    18601 tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg agactctact
    18661 tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag accatgtatt
    18721 tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg
    18781 attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat tagagaagta
    18841 tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct gcttgattgt
    18901 gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag caaaacgatc
    18961 acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag tgctttttgt
    19021 gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa
    19081 tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct tgagtagccg
    19141 acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc gacaaaacga
    19201 tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc tgaccagtct
    19261 acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg agcattcaaa
    19321 taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat cgcatagtct
    19381 gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt aggatctcca
    19441 ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg atgatcaaga
    19501 acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg atcatctgtt
    19561 gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa ggataatgta
    19621 ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc ttctatttcg
    19681 tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc aataatgtca
    19741 tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt
    19801 ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt aattttcccg
    19861 ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact taagtaattt
    19921 tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc atttagcttg
    19981 atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat tgtttctaaa
    20041 actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt tgataaactc
    20101 aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat ttttcccctc
    20161 ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa acaggcgtta
    20221 cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa gggaatcaca
    20281 ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta atgagcgttt
    20341 cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga aggaaactat
    20401 agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat gactaaaaaa
    20461 gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat cgaagagatc
    20521 aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg gtatcgtctt
    20581 gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca aagttctaca
    20641 aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga gtattattgt
    20701 gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg caatcaaatt
    20761 ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac tagccttaaa
    20821 agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta cggaagacgc
    20881 acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa gaataattgt
    20941 cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat tatgtatttt
    21001 gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc tggagactct
    21061 tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa tctcttagga
    21121 aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac gattttttca
    21181 gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa tatgtcctac
    21241 tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt tcaattatta
    21301 acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt gacgatccgt
    21361 gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc tggatttcgt
    21421 gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta tttatctagc
    21481 tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc tgataaaaaa
    21541 atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga atatatcagt
    21601 gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga agatcagctg
    21661 tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat tttgaatgcc
    21721 catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga agaactcatc
    21781 cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc tgataaaaat
    21841 ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa tccaacgaga
    21901 tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa tgagatccgt
    21961 caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt cttccgacga
    22021 ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca ttggagaatc
    22081 agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta acaagtataa
    22141 cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat atttttcgat
    22201 ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt gatggactat
    22261 gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa aatatttata
    22321 aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga cttggctgat
    22381 gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct ttgcgttcga
    22441 taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga ttatacgtgg
    22501 atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct gtcaagtacc
    22561 catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca tgagtaacca
    22621 taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg acttcatatg
    22681 catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt ggctcattat
    22741 ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct ttaggataac
    22801 tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt gttgaatctt
    22861 ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt aaaaaaggaa
    22921 tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct gctcgttttt
    22981 tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa gtcggtgttt
    23041 gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga ccaatgatgc
    23101 tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag acctttgtat
    23161 gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc atttttatta
    23221 acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa acaatccgcc
    23281 tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc aaaaaacagc
    23341 aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa gagcaagcag
    23401 aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga tgaagaaatt
    23461 tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg tcatcacgta
    23521 catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca tcgcatttaa
    23581 ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa tagttaataa
    23641 ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta ttacgatccc
    23701 attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca aagcagtcca
    23761 cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag gaaagatacc
    23821 gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa ttttatagcc
    23881 tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt catgcccttt
    23941 cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa cattttcttt
    24001 gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc ctccttcaat
    24061 gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg catcctccga
    24121 caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt ctgaaagtcc
    24181 attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa taccgacgat
    24241 caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca tttgtttttc
    24301 ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta actctaaagt
    24361 gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga catctttcga
    24421 taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct ttccatgata
    24481 aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta gtagaagacg
    24541 ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag ataacacaag
    24601 tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat cttgcaatct
    24661 tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata cggaaagaaa
    24721 aaatacagac tccagtattg attttgactg caaaagatag tttggaagat aagattgaag
    24781 gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa gaactgatca
    24841 tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat gttctgaagt
    24901 ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa caagttttgc
    24961 ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag ggaatcattt
    25021 tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg acgattacag
    25081 ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga ttggataaag
    25141 aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc aatgaatgga
    25201 gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc atttgctttt
    25261 atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc atacagagaa
    25321 acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt agagatacaa
    25381 agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa cagaacaaac
    25441 atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt gatcttgtgg
    25501 tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa ccaaatcaaa
    25561 gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt aaatgaagac
    25621 agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac gaatcaagat
    25681 ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc attagcgact
    25741 tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat tggtatcagt
    25801 tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag acaaaaagaa
    25861 tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca aaatagtttg
    25921 gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag catcgctcaa
    25981 gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac aatcgctcgg
    26041 aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga atatatcgaa
    26101 caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt tattctccat
    26161 aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt tttggttatt
    26221 ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt ggattcatct
    26281 gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat cagtgaagaa
    26341 gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc gaaagagact
    26401 ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca tcatggacag
    26461 atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt gccgttgaag
    26521 cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt cacagccctt
    26581 ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttct
    26641 ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt ttattgcggt
    26701 cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta acatcttctg
    26761 tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca cgaataactg
    26821 tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg tcatcttcca
    26881 atttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg atgacgattt
    26941 cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt ttgtggatgt
    27001 cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct tttagttgaa
    27061 ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt gtacccattg
    27121 tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc aatggatccg
    27181 ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca gtgccagttt
    27241 ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc attgtatctt
    27301 ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca tcagaaatat
    27361 ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa tcataggtat
    27421 caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca ccatccatcc
    27481 gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt ctagatgctg
    27541 cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta cgcaaccgtt
    27601 caattactga taatgcttct tccctcgtac tatagcttcc gatgaccgtt aaatttttct
    27661 cttccatgta aattcctcct agcaaataga tttatgattg gtatattgtt acttataaat
    27721 ttattctata ggagacgatt gttttctaca aatatttaga ttggaatttg attaaaacaa
    27781 ttta
//
LOCUS       DAZUKZ010000027        27264 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000027 DAZUKZ010000000
VERSION     DAZUKZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.027"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            271..1440
                     /locus_tag="ACG8EF_001112"
     CDS             271..1440
                     /locus_tag="ACG8EF_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4946450.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            1913..2467
                     /locus_tag="ACG8EF_001113"
     CDS             1913..2467
                     /locus_tag="ACG8EF_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4946451.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(2640..3077)
                     /locus_tag="ACG8EF_001114"
     CDS             complement(2640..3077)
                     /locus_tag="ACG8EF_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4946452.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            3125..4072
                     /locus_tag="ACG8EF_001115"
                     /pseudo
     CDS             3125..4072
                     /locus_tag="ACG8EF_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            4343..4778
                     /locus_tag="ACG8EF_001116"
                     /pseudo
     CDS             4343..4778
                     /locus_tag="ACG8EF_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            4794..5627
                     /locus_tag="ACG8EF_001117"
     CDS             4794..5627
                     /locus_tag="ACG8EF_001117"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4946453.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            5872..6897
                     /locus_tag="ACG8EF_001118"
     CDS             5872..6897
                     /locus_tag="ACG8EF_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4946454.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            7035..7160
                     /locus_tag="ACG8EF_001119"
     CDS             7035..7160
                     /locus_tag="ACG8EF_001119"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946455.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            7185..8300
                     /locus_tag="ACG8EF_001120"
                     /pseudo
     CDS             7185..8300
                     /locus_tag="ACG8EF_001120"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            8383..9279
                     /locus_tag="ACG8EF_001121"
                     /pseudo
     CDS             8383..9279
                     /locus_tag="ACG8EF_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            9295..10119
                     /locus_tag="ACG8EF_001122"
     CDS             9295..10119
                     /locus_tag="ACG8EF_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4946456.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            10151..11461
                     /locus_tag="ACG8EF_001123"
     CDS             10151..11461
                     /locus_tag="ACG8EF_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFS4946457.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            11609..12868
                     /locus_tag="ACG8EF_001124"
     CDS             11609..12868
                     /locus_tag="ACG8EF_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4946458.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            12878..13750
                     /locus_tag="ACG8EF_001125"
     CDS             12878..13750
                     /locus_tag="ACG8EF_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4946459.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            13762..14592
                     /locus_tag="ACG8EF_001126"
     CDS             13762..14592
                     /locus_tag="ACG8EF_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4946460.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            14632..16815
                     /locus_tag="ACG8EF_001127"
     CDS             14632..16815
                     /locus_tag="ACG8EF_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS4946461.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            16876..18096
                     /locus_tag="ACG8EF_001128"
     CDS             16876..18096
                     /locus_tag="ACG8EF_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFS4946462.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            18093..19553
                     /gene="gtfA"
                     /locus_tag="ACG8EF_001129"
     CDS             18093..19553
                     /gene="gtfA"
                     /locus_tag="ACG8EF_001129"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFS4946463.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            19630..20736
                     /locus_tag="ACG8EF_001130"
                     /pseudo
     CDS             19630..20736
                     /locus_tag="ACG8EF_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            21058..21267
                     /locus_tag="ACG8EF_001131"
     CDS             21058..21267
                     /locus_tag="ACG8EF_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS4946464.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            21260..21409
                     /locus_tag="ACG8EF_001132"
     CDS             21260..21409
                     /locus_tag="ACG8EF_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFS4946465.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            21563..22228
                     /locus_tag="ACG8EF_001133"
     CDS             21563..22228
                     /locus_tag="ACG8EF_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4946466.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(22512..23597)
                     /gene="xerS"
                     /locus_tag="ACG8EF_001134"
     CDS             complement(22512..23597)
                     /gene="xerS"
                     /locus_tag="ACG8EF_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFS4946467.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            23931..24164
                     /locus_tag="ACG8EF_001135"
     CDS             23931..24164
                     /locus_tag="ACG8EF_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFS4946468.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            24161..24568
                     /locus_tag="ACG8EF_001136"
     CDS             24161..24568
                     /locus_tag="ACG8EF_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS4946469.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(25705..26223)
                     /locus_tag="ACG8EF_001137"
     CDS             complement(25705..26223)
                     /locus_tag="ACG8EF_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFS4946470.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            26590..26838
                     /locus_tag="ACG8EF_001138"
     CDS             26590..26838
                     /locus_tag="ACG8EF_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946471.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         9325 a         4140 c         5037 g         8762 t
ORIGIN      
        1 tggtagtata atttaaaggt aataagttat tcttttatta ctatgtttgg gttttatagc
       61 aaagaaagtt taataagagt ttggacctag caaacaatat taatgaggtc attaaatctt
      121 attatcttta gtgatataac gaaatatcta cacagacaaa atgatagcgt aaaatatttt
      181 gtgtaaatag atatttaggg tagaaaaaaa gaaagtccat tctgtaaaat taaagttacc
      241 acaaccgaaa ttttatagga ggactttctc atgcccaatt ttactacaga aattatggaa
      301 acactaatca ataaaggtga tttagatgaa ttatttcgat gtcatttaga attagctgtt
      361 aattcattat tgcaggctga attaacagct tttcttgatt atgaaaaata tgatcgtgct
      421 gggttcaatt caggtaattc ccgtaatggg aattattcac gttcattcag aacagagtat
      481 ggagaattaa atttggtaat tcctagagat agaaatggta agttttctca acaaacttta
      541 cctgcttata aaagaaccaa tgactcttta gaaaccacta ttattcagct atttcaaaaa
      601 ggaattacta tgtctgaaat ctctgaattg attgaaaaaa tgtacgggca tcactatacg
      661 ccacaaacca tttcaaatat gactaaaata gtttctgaag atattattgc ctttaaagaa
      721 aggtccttag aatccagata ctctgtcatt tttatggatg ctactcacat tcctttaaaa
      781 agacaaactg tttcaaaaga agcagtttat attgtaattg gcattcgatt agatggaacc
      841 aaagaggttc ttgggtttag tcttgctcca actgaatctg cttatgtttg gaaagagatt
      901 cttcaagacc taaaagaccg tggtttaaaa gaggttttat tagtcgtaac tgatggttta
      961 agtggcatta acgatagtat tcatagtgtt tatccaaatg ctcaatttca acaatgttgt
     1021 gtccatatat ctagaaatat cgctcataag gttcgtgtta gtgatcgaca agaaatctgt
     1081 agcgatttca aattagttta tcaagcaagt tcaaaagaag aagcaaataa tcaaataagg
     1141 tttatgatag ataaatggaa aaagcagtat ccacgagtgg ttaaattgct catgaatcct
     1201 gctatattaa ccttttataa tttccctccg tcaatcagaa gaactattta ttcaactaat
     1261 ttgattgaag gttttaacaa acaattaaag aaatatacca aaagaaaaga gcaatttcct
     1321 aatgaagaat ctctggagag atttctagta tctcaattca acaactataa tcaaaagttt
     1381 ctatgccgag ttcataaagg ttttaaagaa atacatgata cattagaatc aatgttttaa
     1441 cttgatcaac agaatgggct tttccattta cacaaaatta ttgacgctcc cgacaaaatt
     1501 atgatcttgc gtaaagttat aaaatattaa taaaaatatt ttattaatga aaagcttggt
     1561 agcacggtta ttcatatgct aatttatttc actttatagt aaggggagtt cttagtatat
     1621 tgttcaaact taaatttcat ataatttaat tatgtaaatt agctattcta agaacttcta
     1681 cggataactg cccgtctaaa atttaaataa gagcttagaa tcttcatata agtaaggtta
     1741 atttttttgt acaaaaaagt cggaatattc tgagttactg aaatgatata aaatcataaa
     1801 ctaaatccct tgattttatt gactttcaaa agtttatata tataagttaa tgattatggt
     1861 aaaatatata cataatcatt aactcagaat atttttaaga agggaaacaa tagtgagcta
     1921 taatgtacaa ccgttaagga caaatgaaga gatacaagat tttcagtttt ggctaagaag
     1981 aacaagtaat ccggaaagag atagtttttt atttttgttt ggaatcaata atgggttacg
     2041 tatgtcagat attataggat taaaagttgg agatatacga ggcaaaagta agcctattat
     2101 tgttgaacat aaaacaggta agcgaaaacc gatttttata gataatttga gagaagaaat
     2161 tttattatat actgaaggta aagaagaaaa tgattggtta tttccgagtc ggcaacaagg
     2221 tcgacatata acaagggatc gtgtctatca gatatatgca gatatagcag aaaaattagg
     2281 acgagatgat ataggaacac atacattaag aaaaactttt gggtatcact actacaagaa
     2341 aactagagac attgctacct taatgtttat ttttaatcat agctcgcaag caattacaaa
     2401 aaggtacatt ggtataactg aagatgaaat aggagcatca cttcgtggat ttaaactagg
     2461 tgtataattt aaaaattttt ataaacagta aagacaaaga ttaaaagcta tttttatcta
     2521 gaacgatttt atttcataaa gttcgcggaa taaaagctgg ggttactggt acttggtata
     2581 ggttcgtgtt atataaacta agtaattaaa taacattttt aggttggaaa aggatgaaat
     2641 taaaaaaaga gaatcaatac ggtctcaaaa acgcaaacgc tgcggaataa gaaaaatact
     2701 gataactcaa cgtttagcag actatctaat agggatagtc tgcttttttc gtttttgacc
     2761 atacttttga ccttctgcaa acttccttgc ttttcctctg tagaactcat gaaatctgca
     2821 aaacgaattg ccgtttcctc tgtctgcttt ttcgcgatat gggtgtatat atcatccata
     2881 gtggttttaa tatctgtatg tccaagtcga acatacactt ctttgacaga cgcaccagat
     2941 tcaaagagca gactacaatg ggtatgacga aaaccatgaa gtgtgatatg ttttaagtta
     3001 ttctctttaa tgatgatttt caaattgtga tttacatagt ctaaataaag gggcttattt
     3061 tcttctgatg aaaacaaaag ttgttcacca tcttttatag gtttgattct catttttaga
     3121 tattatgccc gcacgtattc atcacatttc tattgtgaat cgctttattc gaccgacatt
     3181 tgacttttat cataatatac tcggtttaaa attactaatg aaaacaatta atcaagacga
     3241 tcataccatg tatcacctgt ttttttcaga taatcaccac cgtgtgggaa ctgaactgac
     3301 tttttttgaa gtacaagaag ggaacaacca gttttttgga accaatacga ttgaacgaac
     3361 gatactaaaa gttccttccg aagcatcatt gcatttttgg gaaatatatt ttgaaacaca
     3421 aggcgtttgc cattatggaa tcgaatcatt ttctggacga cctatcttac gttttgaagg
     3481 gccagatgca acacgattag ctttagttcc cttacgcgaa tttgaagata ttgatgatta
     3541 tttcccgtat gtcacagacc agatccctac agagcatgcc attttaggaa ttgatgccat
     3601 tcaactacgc gtacaatatt ccgatgcgac tgaacgagag ttattgtcgg taggctggta
     3661 gcacaaagaa tacgtcagtt tcttttcggg tcatcaacga gtgacaatct tagaaaatca
     3721 agacactcag ttctatcaag aagttcagat tattcaagat cgtgagaatc cagttgccat
     3781 tgaagggatt ggtagcgtcc atcatgttgc gtttggcgta gaaaataaat ctgatttaca
     3841 acgcatcgac aaacaattac aagagcgcaa ttttataaat tcaggaataa aagatcgtga
     3901 attttttgta tcactttatt atcgtgaccc aaatcaatta ttgattgaaa ttgctacagg
     3961 ggaaggaaac ctagatgcaa aagcgtatga aaatcaaagt ccacgctttg aagaaattcc
     4021 tttattttta ccgcagtact taaatttcat tcgtgaacaa gtggagcaat aattagctaa
     4081 tcaaatccat gacgaagagt aagaggttga ctcataagtc aaaaaataaa gaaaaacgca
     4141 tcaaatccat tctaaagaat gttgatttga tgcggttcct atttttataa actatcattt
     4201 tcgactaata aaactactta tgagtcagcc tctctttttt tcttgatttt tatacctctt
     4261 tctaggtaaa ctaaacatct gccaaaaata ccgtgtctca ttgaaacatt ctcaactatt
     4321 tcaaaataaa ggggagtacc aaatgaacga gtatattgaa attatccacg caagagaaac
     4381 aacttaaaaa tgtcagtctc aaaattccca aaaataaaat tagtattttt accagcgtat
     4441 ctggttcagg aaaatcatcg attgtttttg atactattac ccaagaagct ggtcgtcaac
     4501 tgaatgacac ctatgatagt tttacccgtt tatttttacc taaatacagt cgtccagagg
     4561 ttgatgagat tgataatctt tctacggcga ttgtggtcaa tcaaaagaag attggtggca
     4621 gtgcgcgctc aacgttaggg acgattacta acatcaatgc gttatttcgt gtgttgtttt
     4681 catgttttgc aacacctagt ttaggatatg caaatgctta ttcatttaat gatcctagtg
     4741 gaatgtgttc tgtctgtcag ggaatcggta agacgattgc atttttaata ggattgcttt
     4801 atttttcggc tattgataat gctgattttg tattttctga tccatttacc gcagaagttt
     4861 tgtctggaat tgaggaacag gcaaagaatc atggttattt tgtattggtg cataatgtta
     4921 attcagcaga agatgttaaa actattcaga gaaattggcg ttttgagggg ttcattgttg
     4981 ttggtgtacg tgtaaaagaa tttttggaat taaataacgt ttttgatggt cctgttgttt
     5041 atattgatac gcatctttca aaagagacat taagagaact tgaacaacaa atgaatcgtt
     5101 tatttgtaaa tacaaatgat taccaagcta gtacattggc agtggaacat ttgattcatt
     5161 atggtcatga gaaaatagcg tttctttctt ttgactacat tgtaggtgaa ccaagtgtta
     5221 ttcaagaacg acatcaagct tatatcgata cattaaagaa atatcattta acctttaaat
     5281 cgcattggat atataacaac tctgaatttg agcgaattta tcaagaatta gctgaattta
     5341 ctgcaattgt tgttactgga gattatttag cggctaaatt agtaaaattt ttaaaaaata
     5401 agaataaacg agtgatggaa caattatcga ttattggatt tgatgatatt acttttgcag
     5461 aattaatgga tcctgcgttg acaacaatac gactaaatcc aggtaacaag ggggcgattg
     5521 cttttcacca actgattgga ctggccaaac aagaagaaat aaaaaatcaa gtactactat
     5581 tagagggaga attgatttct cgagactctg ttcacaaaat caaataaaaa agctctgtga
     5641 aataagtata gttaacaata aaatatccga acgatagaga gaatgaactg tgatctagca
     5701 gttcctcgtc gcaaattcac tagaattagc attatttact ctaaattgtt cggatatttt
     5761 ttcttgtttt taattatgat tacagtgcct atttttaacg aaatttgctt attttcaaaa
     5821 tgtatgatta tatgttagta aacactttca aatatgaaaa gaggagctat tatggtagga
     5881 attagggatg ttgcaaaacg ggcgggtgtg tccattgcaa cagtatcgcg cgtgctaaat
     5941 gaggatccga cactttcagt aaccgatgaa accaaacgaa agattgcaga atcggttaat
     6001 ttttatcatt atgtcaaaaa aagtcccgtt gcaaaaaatt taacaaatat tgcattaata
     6061 acgacagttt ctgaagtaga tgagttggaa gatccctatt ttcgaacgat tcgcagaggg
     6121 attcaagtcg aagcagaaca aagcaaagtc aatttgaaga aaattattcg tttgtcagaa
     6181 acaccgttgg agcaagacga tttgctaaaa tgtcaaggtg tgttgattat tgggcaggtg
     6241 ctatcttctg tgattgaaga agtatcagca attaattcga atgtcgtagt gattgatgac
     6301 cctagtattg aagctgaagt agatgcagtt tatactgatt taacaaaagc aacgaatcag
     6361 catttagatc gattgtataa aaaaggacac cgaaacattg cgtttatcgg aggaggacgt
     6421 gttcgtttag atagccaagg aaataaacac gtgtcagatg atgattatcg aaaaatagcc
     6481 tatgagacgt ggatgaaaca aaaaggatta gaaaaatata gtcaaattta tttagaaggc
     6541 tggaccacac ttgacggaat gcaggcagca gatttacttt tgaaaaaaca tgctaaagat
     6601 aagttgccga ctgctatcat ggtaggaaat gatcctattg ctgtgggggt ttatcgttca
     6661 cttcaaaaaa atggtctaca tattccagaa gatatttcga ttgtgagttt tgataatatt
     6721 gaagttgctg aatttttaac acctgcatta aatacggtga atgtgaatac agaagaaatt
     6781 ggaaaactag cagttcgcat ggttaatgaa cgaataaaag aagttagaaa tgtttcgatc
     6841 aaagtgattg tttctaataa tattattgtt agagaaagtg aaaaaaatat cgattaaaaa
     6901 agtaaacatc caaaaaaggg tgtttacttt tttgtttact aatattttta gtaaaaaatc
     6961 tcgacaaaac attaagtaaa cagttatggt gttgttaaca aaaagaaaac gcattcaaaa
     7021 caggaggaat taagatgaaa catttcaaaa aaatagcttt aacagggata gctgttttaa
     7081 ctgggacatt actcgcagca tgtggtgggg gagatagtaa ttctaaagac gggcaagtaa
     7141 ctttaagcta tgcgagttga gatactgacc aagctccagg actaagaaaa atgttagatg
     7201 aatttgaaaa acaaaatcct gatatcaaag tagaaatgga gacaacgcct tgggatcaat
     7261 attgggttaa attagaagct gcaacaacgg gaggtaatat gccagatgta gtaaccatgc
     7321 actctagcga atcctataaa tatatgtcac agaatgcttt gatgggactt gatgatgtta
     7381 tcaaagaaaa tgatattgac atgggaaact ttactgaagg aattgctgat ttctatacgc
     7441 ttgaagacga actatacgca atccctaaag atgcttctgt agtggggcta tggtacaaca
     7501 aagaattatt tgataatgca ggtgtcgatt atccagatga aacatggaca tgggaaacac
     7561 tcagacaagc tgcaattgat ttaacagatg aatcaaaagg catctatggt tttgcatcac
     7621 atattggaac agagactggc tactggccat ttatttatca aaatggggga gaagtatttg
     7681 ccgatgacaa tacaaaatca ggtttgaata ccccagaagc acaagaagct ttagcttttt
     7741 atcgagatat tgcacttgtt gatcatgctt caccaacaat tacagaatta caagatcctg
     7801 ataagttaag tcgttttcaa tcaggtagat tagcaatgat gattgaagga aactggtttg
     7861 caccgggatt tttgaaaaat gactatctag ttgaaaatgt tgcttctgcc gtgttaccta
     7921 agggaaaagt taatgcaaca attacaaatg gacttggttg ggctgccagt gcgagtacaa
     7981 aatacccaga agaagtgaaa aaactaatgg catttttagc tagtgaggac gccaataaaa
     8041 tccaagcgga aacgggggca tcaatcccag cactaaaagg ctttgaagac gagtgggctg
     8101 cttcttcaga tacaattgat ttgagtgctt ttggaaaaat gttggagtat ggagtccctc
     8161 gtccatacaa taaagatgga ttacgagctg aacaagcaga agttcaaatt attaaccgta
     8221 ttctgtctgg tgaagtaact gttgaagatg gaaccgctca aattgaaaaa gaagtcaatg
     8281 cgatattgag cggaaattaa attagttaat tatacaatat acaggcgaaa ggattctatt
     8341 tcgtctgtat attaatggat gaataccaaa aagggggata ttatgaaaaa tcgaattttt
     8401 ggaaaatatc gtaaagatca agtgatatgg ggctatattt ttatttttcc aacggcatta
     8461 ggattaattt tattaaatat atggccagca attcaaacag caattttaag ttttcaaaaa
     8521 acggtagggt tcggtgatac tgaatgggtc ggattaagaa attaccaaaa aatgtttgca
     8581 gacggtgaag tttttcagtc tttattaaac acattaattt atgcagtggt ttcgatacct
     8641 agtattgttg tattatcctt gttggtggca gttttaatga atagaaaagt taaaggattg
     8701 tcgatttatc ggacaattta ttttctacca gtcgttgctg caccatccgc agtcgcaatg
     8761 atttggcgct ggatgttcaa taatgattat ggactgatta ataacatgtt ggctaaaata
     8821 ggtctcgatg gaccagcatg gttaacggat ccatcgattg cgatttattc tattatcatt
     8881 gtaggagttt ggagtgctat tggttataac atggtgttat tactagccgg cttataagaa
     8941 attcctaaag attattatga agcagcggaa attgatggtg ctagtccaat ccagcagttt
     9001 ttcaacatta cgttaccatt agttacacca acattatttt ttgtagttgt aacaacagtt
     9061 atcaatgctt tccaagtatt tgatgttatt tttatgatga ttactcctaa tagtaccgcc
     9121 atgtttaaat cacaatcatt agcctattta ttctataaac attcatttat tttaaatgac
     9181 aagggatatg gttcggcaat cgttatgttc cttttagtga ttatattaat tattactgca
     9241 attcaaatga agttgcagaa aaagtgggta aactattaag gaggctatca aattatgaaa
     9301 aataaatttt tttcaaatgc gccaatccat atcatgctta ttttaggtgg tattgcaatg
     9361 gcattacctt ttatttggat gattttaact tcagggaaaa cgttggttga atctacccaa
     9421 attccaccta caattttccc tgagaaattt cagctaagta actatcaaca agtacttgat
     9481 ttattaccat tcatggcttt ttatatcaat acatttttaa tggttgttgg acgagtgatt
     9541 ggctcggttt tattttcagc aatggctgca tatgcttgtg cgcgtctaag ttttccaggg
     9601 aaaaatatcc ttttcagctt agtacttatt caaatgatga ttcccggttc catttttatt
     9661 actccacagt atttattagc acaaaaattg ggtattttaa atacaattcc agcattaata
     9721 ttaccagggg tagttagtgc gtttggtacg tttttattaa gacaattttt cttaggttta
     9781 cctgatgaat tggaggaagc ggcctattta gaaggtgcca atatttggac gatctttttc
     9841 aaaattatgt tgccgcttgc taaatcagga ttggttgcac ttggaatatt cacagcgttg
     9901 tttgcattta aagatttaat gtggccattg attgtcaata cttcaattga taagatgcct
     9961 ttagctccgg gattagcaaa cttacaagga caatatgcca cgaacttccc acaattaatg
    10021 gcaggttcgt tattagcaat ttggccaata gttgtgatat tcatcgtttt ccaaagaaaa
    10081 ttcattgaag gaattgctac aacaggtagt aaattataaa actattaatt agtgattgga
    10141 gagtaagtac atgacaaaaa ttacatttat tggtgcagga agtactattt ttgcaaaaaa
    10201 cgtattaggc gatgcgatgt taactccaag tctacaagat gcacatattg cattgtacga
    10261 tattgatgaa aaacgactaa aagaatcaaa tttaatgctt aaaaatatta ataaaaattc
    10321 gaatgaaaat cgtgctgata ttgtttctta ttatgatcgc aaagaagcgt taagagaggc
    10381 tgattttgtt atcaatgcta ttcaagtggg tggttataaa ccttcaacag tgattgattt
    10441 tgatattcct aaaaaatatg ggttgcgaca aactattggt gacaccatgg ggattggtgg
    10501 gatttttaga accctacgga cactaccagt aatgttagac tttgctaaag aaatggaaga
    10561 agtttgtcca aatgcttggt tattgaacta tacaaatccg atggcgatgt taaccatggg
    10621 tgttttaaaa gcgacgagta ttaagactgt tggtttatgt catagcgttc aagtatgcgt
    10681 accagaattg tttgagcatt taggaatagc cgatcaatat gacttaaatg actttcaatg
    10741 gaaaattgca ggcattaatc atatggcatt cttgttggaa attacaaaaa atggggaaga
    10801 tttctatcca acaattcgtc agttagctgc agaaaaacca aatccacatc cagattctgt
    10861 acgttttgaa ttgttgaaac gttttggtta ctatattaca gaatcaagtg aacacaatgc
    10921 agaataccat ccatacttta ttaaatcaaa atacccagaa ttgattgacg aattagggat
    10981 tccgttggat gaatacttac gtcgttgtga acaacaaatt aatgattggg aaagcatgcg
    11041 agaggacatt gtaaataatg gggagttaac acatacgaga agccgtgaat atgcttcata
    11101 tatcatggat tctattacga caggaactcc gtcagtgatt gcaggaaatg tcttaaataa
    11161 aggcttaatc acaaacttac cgacagatgt ttgtgtggaa gtaccttgtt tagtcgataa
    11221 aaatggcgtg caaccaacct atgtcggtga attgccaaca caattagctg ctttaaatcg
    11281 gacgaatatc aatgttcaag aactgacagt agaagccgct ttaacactag ataaatcaaa
    11341 aatttatcaa gcagcgtatt tagatccaca tctatcatct gaattgtctt taacagatat
    11401 caaaaattta gtggatgatt taatcgaagc acatggggac tatttacctg aatataatta
    11461 aaaagaagct gaaagagtaa ggaattttca attccttact cttttcttag ttaaatttag
    11521 taaaaatatt gacaaaacac ttagtaaaca gttacattag tgttaacaaa aagaaaacgc
    11581 attcaaattg tataaggagg agcataaaat gaagtggtct aaaaaaatta ttttagcttg
    11641 tatagcacta ggagcaacga tctctttgac tgcttgtgga aaaaatcagg aagatgcaag
    11701 cggtaaggta gaaatagagt tttttaatca aaaaaaagaa atgactcaaa cgattcagga
    11761 gattgctaaa gattttgaag caaaaaaccc tgatattcat gtaaaagttg tggatgtacc
    11821 gaatgctggt gaagtaatta aaacacgtat gcttgcaggc gatgtaccag atgtcattaa
    11881 tctttatcct caaagtatag aattgaaaga atgggcgaaa gctggttatt tagaggactt
    11941 aaccgaagaa ccgtatttag agaatatcaa aaatggatat gcacaacgtt ttgctattga
    12001 ggatcgtgta tatagcatac cattaaccgc taatgtctat ggtttttact ataacaaaac
    12061 tgcttttgag gaaatgggga ttcaagcacc agaaacttgg gcagaatttg aaaaaatagt
    12121 agctgagatt aaagatcaaa acaaagtacc gtttgcgatt gcaggagcag aaggctggac
    12181 tttaaatggt tatcaccaac tagctatagc gactgctgct ggtggtgaag aggaagcgaa
    12241 caatgtttgg cgtttctcag aagttaatgg aattaatgct gaaagcaaag aaatgcaaaa
    12301 agattttaat cgattagatt tgttaagaga accaaaagct ttgcaaaaca actggcaagg
    12361 tgccggttat aacgatacag tagttacttt tacaaaaggt gatgcgttga ttatgccgaa
    12421 tggttcatgg gcaatgccga tgattcattc acaaaatcca gattttgaag taggaacttt
    12481 tccttttcca gcagatgaag caggtcaaag tctaacgatt ggcgcaggag acctagcctt
    12541 atcagtttct gcaacttctc aacataaaga agaagccaaa cgttttgtag aatatatgac
    12601 aacacctgaa gcgatgcaga aatattatga tgtagatggt gcaccgtgtg ctgttgaggg
    12661 cgttatcgag aatactcagg attcaccatt aagtggttta actgaacttg cttttactga
    12721 tcgtcattta gtttggttag ccaaagattg gaatagtgaa aatgattttt ataccttaac
    12781 aacaaattat ttacacaatg gcaatcaaaa agccatgatt aacgcattaa atgctttctt
    12841 taatccaatg aaagctgacg gaaggtagga aaataaaatg aaaataaaag aaattttaaa
    12901 tcgttactgg gcaatcattt ttgtagcaat tccaattatt ttgcagatga tttttttcta
    12961 ctttccaatg gttcaaggtg ctttttacag tttaactaat tggactggtc tgacttataa
    13021 ttttgatttt gtgggtttga acaattataa gattcttttt tctgatccga agtttatcaa
    13081 atcgattggc ttcactttaa ttgtgacgct ttgcatgatt gctggacaaa ttggttttgg
    13141 tattttgatt gcgcgggcgt tgaacgcaaa tattaaaggg aaaacattct ttagagcatg
    13201 gttctttttt ccagcggtct tatcgggcgt ggttgtttct ttagtattta aacaaatttt
    13261 taactatggt ttaccaaaaa ttggtagtat gttaggcatt gaatttttac aaaccagtct
    13321 tttaggaaca acatggggag cgatttttgc agtaattttt gttttacttt ggcaaggaat
    13381 tgccatgcca gtcatcattt tcttagcggg ttttcaaagt attccacaag aaattattga
    13441 agctgctaga atggatgggg cgaatgataa gcaaattttt tggaatattg aaatcaccta
    13501 cttattacca agtatttcaa tggtatttat tatggcatta aaaggtggct taacagcatt
    13561 tgatcaaatt tttgctttaa ctggtggtgg accaagcgat gccacaacat ctttggggtt
    13621 acttgtttat aattatgctt ttaaaaacaa tcagtttggt tatgcgaatg ctattgcttt
    13681 agtcttattt atcttaattg ggattgtttc actggcacaa ttaaaattat cgaaaaaatt
    13741 tgaaatctag gaggtttccc aatgaaaaca tcaaaagaaa aaaatatttc taaatacttg
    13801 cttttattat tagggggctt catgatttta gttcccttag tttcaacgct attcggttca
    13861 tttaaaacaa cacccgatat tatgaataat ttctttcgtt ttcctaatcc agcgactttg
    13921 gaaaatttcc gaagattgtt agcggatgga attggtcatt acttttggaa ttcggcaatc
    13981 atcacaatca ttgcagtagt tgctgtgaca gcaattattc ctatggctgc ttattcacta
    14041 gcaagaaaaa tgactcgtgg gaaagcatac ggattgatgt atttattttt aattttagga
    14101 atttttgttc ctttccaagt aattatgatt ccgattaccg ttatgatgag taaaattggt
    14161 ttagcaaata tgtggggatt aatcttactc tatctcactt atgccgtacc acagacacta
    14221 tttttatatg ttggttttat taaactaaat gtgcctatca gtctggatga ggctgctgaa
    14281 attgatgggg ctagtcactt tacaacttat ttcaaaattg ttttcccaat gttaaaacca
    14341 atgcatgcga cgactattat tcttaatgct ctatggtttt ggaatgattt catgctacca
    14401 ttattaattt taaatcgtga ttctaatatg tggacactac cattgtttca atataattat
    14461 acgggacaat attttaacga ctatggtcca agttttgctt cctatacagt aggaattgtc
    14521 acgattacac tagtttatct tgctttccaa agaagtatta tagctggaat gagcaatgga
    14581 gcagtgaaat aaccaataaa ttattctcat tataaaggag ttcataaact tatgattata
    14641 tttgacaatc acacacaaac atttcattta caaaataaaa aaattagcta tctattagga
    14701 atcgaagaaa ataattattt aacgcatctt tattttggaa aaaagattac gcactattct
    14761 ggtgggtatc gctacccccg aacagatcgt tccttctcac ccaacaatgc aaatgcgcaa
    14821 gatcgtttat tatctttaga cacattacca ttagaatttc caggatatgg acatggtgat
    14881 tttagagaac cggcgcataa tattaaatta ggaaacggta gtcgtgtaaa tgattttcgt
    14941 tatgatagct acgaaattca aaaaggaaag cccagcttag cgggattgcc agccttatat
    15001 acggaagatg atgatgaagc tgaaacgctg attattcaat tgaaagacgc gattagtgga
    15061 ttgactttac agttgttcta cacaatttat caaaatcaca gtgtaattat tcgttctacg
    15121 caattagtaa ataatagtga tgaaacagtg gagattaacc gcttagctag tcaatcaatt
    15181 gattttccaa atcgtccact cgagttaatt catttaaatg gtacctgggc aagagaacgt
    15241 caaatgacta gagaaaaaat tcatacaggg ataaaagtat tagatagtaa acgcggatca
    15301 agcagccatc aacaaaatcc gtttgttgct ttagtagatg ccaaaacaga tgaatttcaa
    15361 ggagaggcat atggatttaa tttagtttat agcggtaatc acgaaacggt gattcaaaaa
    15421 gatccatttg accaaacacg ggtcattatg ggaattaatt catttaattt tggttggcaa
    15481 ctaaaaactg gcgactcatt tcaaacgcca gaagtcgtga tggtttatag taatgaaggt
    15541 ctgaatacgt tatcacagac gtatcatgaa ttgtataatc accgtttaat tcgtggaaac
    15601 tatcgtttaa aagaacgtcc cacgttaatc aacaactggg aagcaacgta ttttgatttt
    15661 aacgaagaaa aaatcatgaa tattgtcgat gatgcgaaga acttgggcat tgaaatgttt
    15721 gttttagatg atggttggtt tggcggtcgt gaaaatgact tcacttcatt aggtgattgg
    15781 gttgaaacag aaggcaaact tgcttgtggt ttggaaaatt tagccaagca agtgcatgat
    15841 aacgggatga aatttgggat ttggtttgaa ccggaaatga tttctgaaga tagtgaacta
    15901 tttcgagcac atccagattg ggcattgggt attccaggtc gtgaccgctc aattagtcga
    15961 aatcaatatg ttcttgattt ttcaagacaa gatgttcgtg ataatatcta tcaacaaatg
    16021 acggcgattt ttgatcgtgt accgattgat tatgtgaagt gggatatgaa tcgaaatatg
    16081 acagaagtct attctgtctt gttggactca acgatgcaag gtgaagtggc tcatcgttat
    16141 atgttagggt tatatgaatt tttagaaaag ttaacaacga attatccaac tattttattt
    16201 gaaagttgtt cgggtggagg cggtcgtttt gatgcaggtt tcttgtatta catgccacaa
    16261 acttggacga gcgacaatac cgatgccgtg gctcgtctga aaattcaata tggaaccagc
    16321 ttagtgtatc caatttcaag tatgggtgcg catgtttcag cagtcccaaa tcatcaaaca
    16381 ggaagagaaa cgagtttaac tattcgtggc aatgctgcga tggctggtgt ttttggttat
    16441 gagctagatc taacgcaatt aacttctgaa gaaaaagcag aaattaaaga acaagttgcc
    16501 ttttataaaa agcatcgcca acttttgcaa tatggaactt tttatcgctt ggaaagtccg
    16561 tttgaaggca atgatactgc atggatgttt gtctcaaaag accaaacaga agccatggtc
    16621 ttttatttcc gagtattagc agaagctgcg catccattag tcacattgaa attagcagga
    16681 ttagcagaag aagcaactta tcaaatggaa gatgtaacat ttgctggcga tgaattgatg
    16741 aatctcggtt tttatattga ctcagaacta catggtgatt atgcgacaca acgcttttat
    16801 ttgaaacaga tatagtagct tgaatttaca atataagtgc aacaccaaaa gagactcatt
    16861 ccaaacgaag aaaatatgtt ttatttacac gcatttacta aaaagcagcc tgatttgaat
    16921 tgggaaaatg aagaagtacg tgaagaaatc tatgcaatga ttaactattg gttagataaa
    16981 ggactaggcg gatttagaat cgatgcgata ttaaacatca aaaaaaaact agagtatggc
    17041 atctttgaat ctgatggaga agatgggtta gccttcattg ggccctggat tcttaatcag
    17101 tcgggcattg aagtgtggtt aaaagaaatg agagagcgga catttaaacc acataatagt
    17161 atgacggtag cagaagccga tgttcctaat gaacgtttag atgaatatat tggtgaagat
    17221 ggctattatt cgatggtttt tgattttagt tatacggata ttgatgtacc tgaaactggt
    17281 gaatggttta aagatagcca atggacatgg acggatatgc gtaccaatat ttttacgaat
    17341 caattagtta cgcaagataa aggttggggc gccttatatt tagaaaacca cgatcaacca
    17401 cgttcaatta ataaatatat tccagaagaa tggatcaatg attatagtaa aaaaatgttg
    17461 gcgacgcttt ttatgttact aagaggtacc ccttttattt atcaaggaca agaactagga
    17521 atgaccaaca tagaaatggc atcattagaa gattttgatg atgtagctac gcattctcag
    17581 tataaacggg cattagaata tggattggca ccagagcaag cattgaaagc tgtcaataaa
    17641 cgaagtcggg ataattctcg cacacctatg caatggaata cgcaaaaaaa tgccagattc
    17701 tcaacttctg ataatgtatg gttaaaagta aatcctaatt atccagaact caatgcagaa
    17761 gctcagttaa ccaatcaaca gtcggtattc aattattatc atcaattaat taaattaaga
    17821 aaaaatagtg tatacaaaga cgtgctaatc tacggacaat ttctaccagt gaaagataaa
    17881 aatgaacata ttttgcttta tgaacgccaa ttagaagata aaatcgtatt agttatctta
    17941 aacgttacag caacagaaca agaatattat gtagatgaac agtaccaaca agtattaatc
    18001 aataattatg aagatgtttt attagatcaa agtcgaaaat tgcgtttacg accgtttgaa
    18061 agtattgtat tagctaatta tggaggacaa ttatgaaaat taaaaacgaa gcaatgctca
    18121 tcacctatgc agatagttta ggaaaaaaca tgggcgaatt aaaacaagtc ttagacaaac
    18181 atttacaagg tgttattggt ggtgtacatc tgttgccttt ttttccatcg acaggtgatc
    18241 gaggctttgc gccgagtgat tatacgacag ttgatccagc tcttggaaca tgggaagatg
    18301 tggaagcatt aggtgaagat tattatttga tgtttgattt tatgattaat cacatttctc
    18361 gtgaatcaaa atttttccaa gatttcaaaa aaaaccatga gcagtcacca tacaaagaaa
    18421 tgtttatccg tattcatgaa tttttcccag aaaatcgacc aacacaagaa gatattgatt
    18481 taatctacaa gcgtaaagac aaagcgccgt ttcaaacggt agaatttgcg gatggtacga
    18541 cagaagaggt ttggaatacc tttggggaag aacaaattga tttagatgtg accaaagaag
    18601 ttgtgaaaga atttattcga gaaaccatca aagatatggc gaaccatggc tgttctttaa
    18661 ttcgattaga tgcgtttgct tatgcgatta aaaaattaga taccaatgat ttcttcgtgg
    18721 aaccagatat ttgggaactt ttagacgaag tgcgtcaaga agcagcgaaa tatgacgtcg
    18781 aattattacc agaaatccat gagcattatt ccattcaaat gaaaattgcc aatcatgatt
    18841 attatatcta tgacttcgcc ttgccaatgg tcactctgta ttcactttat agtggaaaat
    18901 cagaacgttt agccaattgg ttgaaaatga gtccgatgaa gcagtttaca acattggata
    18961 cgcatgatgg aattggtgtt gtggacgctc gtgatttatt aacagatgaa gaattggatt
    19021 acacctctga ggaattgtat aaagtcgggg cgaatgtgaa gaaggtctat tcaagtgcca
    19081 attacaataa tttagatatt tatcaaatta acagcaccta ttatagtgca ttaggcgata
    19141 atgaccgtag ctatctgtta gcacgtgcga ttcaagtatt tgcgccaggg attccacaaa
    19201 tttattacgt tgggttatta gcaggaaaaa atgatattga actgttggaa gcaacgaaag
    19261 aagggcgtaa tattaatcgt cattactatg atttagccga aattgaagaa gaagtgcaac
    19321 ggccagtgat tcaacaatta tttgcacttt taaaatttag aaatgaatct gcggcgtttg
    19381 atttggatgg aacgattgaa gtagcaacgc catcagaatc agaaatagtg attacacgta
    19441 aaaataaaaa cggtagtgat agtgcaacat taacggctaa cttggatacg aaagaattta
    19501 tcattgagtc taaagatata aaaattttca ctagtgttgc attaaaattt taacctattc
    19561 catagaacaa aaaatccttt atactagcac ttggatgact tatccaagac caataaaaag
    19621 gactcataca tggataagta taaaacaaat acagcattta ataaatggtt ttctgccata
    19681 aaattaggaa aactttctgt caactcccaa aaatgtattt ttgattacaa ttcgtatcgt
    19741 aaaaaactgt cttttgaagc tgtgctcaag ctctttctct acgcagttaa tgaagaaaaa
    19801 gaaagtttgc gtgatttgag tacagctctt atcgaccgat cgctccaact agaaaccgat
    19861 gtgtcatcga ttagtcatac ccaattgtct cgagcattta atgcacttga acctaaagtg
    19921 ttagaagaaa tttttcaaca actgttagaa aaagttcaac atcaaatcaa accaaccaag
    19981 cgaaatcatc tgtatctgat tgattccagt acgttttcgc ttagtttaaa acgtcataag
    20041 tgggcgactt tccgtcaaac aaaatcaggt gtcaagttac acttaaacta ttgttatatg
    20101 gacgatacta cgatgtatcc aactgatttt accttaacaa atgcccatga acatgatctc
    20161 aatcaactac cagtattggt gaatcaacca gaggcaactt acgtgtttga tcgtgggtat
    20221 taggactttg aaaagatgga tcagatgcat tgggatggct atttcttcgt cactcggatt
    20281 aaaaaaaaca ccaaagttca cgtgatagat actctggaaa catcgccaga aacagaaatt
    20341 ctacgagacg agctggttcg tttaggatct aaaacctatc taaccgccaa tttccgacta
    20401 gtcactgttc aagataagaa cggacgtgtg tttcaattta tcaccaatcg aatggacgtc
    20461 tcttcaaaag aaatctctga tatgtatcac gctcgttggc aaatcgaatt gtttttcaag
    20521 cacatcaaac aacacatgac cattaaaacc ttcttttctc aaagtgaaaa aggcgtgcag
    20581 aatcaattga ttttaacgat gatttccgct ctcctcacgt ttctcatcaa attggagacg
    20641 aaaacggaaa aatcagtgtt ccaaatcaaa cgattctttc gttatttact gtttcagccc
    20701 tttgaatgtt gtctagaaaa attaattccc acatgagaaa aaagaaattt ggctcgttac
    20761 ttttggtttt cagttgaata acagataaaa aattttctgg aaaaagtcat cctctgcgtc
    20821 agcacagttt tttttgactt attttcagag gaaatagttt tcagaatatt ctaaaatgtt
    20881 ttatgcaaca ctagtgatat tttttagaat tagatttcta atgtgcattt tttgagtatt
    20941 aaggagcaag tatacaaaga tttataaagc tgattaaatt tgaataaaaa tatgcaggaa
    21001 cgctactatt tttagtagtg ttctttttta tatttgaata aaaaatgtgg tggttaaatg
    21061 gtggttataa caaaaaatga attaatgaaa ttaggttatg ggaagtatca gtctgagaga
    21121 atcatacgac aggcaaaagc aattatggtt tcaaaaagct atacctatta cgaaaataga
    21181 agattaggac tagtacctgt tgaatctgta gaagaagttt taggtatgaa gatagaggta
    21241 gaggaggatt ttcagaatca tggttaattt taaaaaagat aaaaatggga aatatcattt
    21301 tactgctagt ttgggattcg acaaacgaac agggaaaaga gtacaaaaaa acgtagaggg
    21361 gttaaaacag ttaaagaaga aaaaaagcgt ataccgaact tgtttctaat tatggaaaag
    21421 aagcagaaac ttctcaaagt actatgttgt ttgaggagtt tttttataaa ttattcctac
    21481 cttattatca agggaaagta gaagaaagaa catataaaaa tcggttatct acaatcaaaa
    21541 aaacttttct tatttcttta aaatggagtt aagagaaata cgtccaattg acattcaaaa
    21601 gtggcaaaat aatttattga gcaaatataa aaattcttat gtaagaaaca tgtatgggat
    21661 ttttcagatg tcattagatc gagcagttgt tttgggacta cttgaaaaaa atcctgcaaa
    21721 agttattgga aatgtgaaga aaaggaaaac tacagttgat ttttggacaa aagaagaatt
    21781 tacaaaagtt atttcaactt tttatattga tgatttgtac caacattttg gttttatttg
    21841 tatgtggtta ctttttatga ccgttatgcg tttgggagaa gccactgcat taacttggaa
    21901 agatattgat tttgaaaata aaaacttgcg tgtgaataaa acactctatt ataagagagc
    21961 caaaacctat gagtttgtag aacccgaaac ggcttctagt attcgtacaa tcgaactaga
    22021 cgacaataca attacctttt taaaagcttg gaaggagcgt caagtttcaa taggaggcat
    22081 gtcgtttgta ctttcttata atggaacacc tactcagaaa tatacaatcc gtcatattat
    22141 gagaagacat gcaacgatcg ttggtgttca tataatccgc atacacggat taaggcatag
    22201 tcatgcttct ttgcttatat cacaataaaa agatgcactt cgaattaaag aaaggttaaa
    22261 acatgctgat gtacaaacga ctttaggaac ttatggtcat ttgtatctaa attcattgag
    22321 agagataaca aatgatccca cagattatat ccaaactaat ttttctaaaa tcaaaccgac
    22381 acctatttac gataataaaa aataaatgat aaacaaaagt aggatcacac caaaagaaac
    22441 acactagaaa aactataata caggcttttc tagcgtgttg tttcttattt ccgcctattt
    22501 atacattata ctcataaagc atttaatgca tcgatcgtag ccgcattgtc tatatgggta
    22561 tataaagttg ttgtattggt acttgaatgt cccaattgtt gggcagttaa taccaatgaa
    22621 tttgtctgtt ggtagagtct agtagctaat gtatgacgca gtttgtgagg gctgacttga
    22681 attctaaagc cttttgaata ttttgctacc atccgttcga ttgcaatacc ggatatacgc
    22741 ttggcttctc cttggtaaat tgctagaaat aaagcttttt cgttttctgt tactttatat
    22801 cgttctttcc gaatagctag ataattagct aaatattcca ttgcgaatgc tgctatattg
    22861 acagcatctc ttttatttcc ttttctagtt actaccacat tattcttatc taaagatagg
    22921 tcatctaaat taatatttat aacctcggag agtctcaatc cagatcctaa aatcagtgca
    22981 ttgatcgcca ggtctctttc tacatctcgt tgataataga tcaaagctcg tttactcaaa
    23041 gtatttttat attcattggc aatataatct agataatttt gtgtatcctt atctagaaat
    23101 aattttttct caatggcatt ggcacgtgca gctaacgtag ttttatctgt gatggttgct
    23161 acttgatcca taggattatt gtctaagtat ggttttccta gaggattatt ggaacttttc
    23221 gaaagatatt taaatagaac ctttaatgcc gtgatatttc tattgatagt agaagtagat
    23281 agacctaagt tattattatc tttgagctta gtttttgttg tctctttttt tacattttct
    23341 tttgtgcgac gtttcaaatg agatttgtac aagtttaggt cataggcagt taactgtctt
    23401 atcgaggtga tagaagtatc aggaacgata gactcactca gaagccattc ataaaagagc
    23461 ttataatttt ttagatattc taaagtggtt cgaggtgatt gatctttatc ctcacgatct
    23521 tgaacaaaat cttttatata atcgggcata tgaagtatga ttgtatctat tttttgatat
    23581 atttctgtat ttttcatgtc attcatcctt tcaataagta ggggagaaga gtatctctat
    23641 aattggacta taccaattat agaatgtttt gtcaattggt atgctctaaa atttattaat
    23701 ttgtttcaaa aaaagcatat aaacaggtat acgtttaata atttaaaata aatggtatac
    23761 ataaataggg aaggaaatag aagggagcaa tctaaatttt ttgaattttc ttaattaatt
    23821 atatagaaag aacaatcttt ctatagagtt agacatgaga gggactatgc ttgatttaaa
    23881 atcaacaagc gtatatactg tatatacaaa gaaaaaggag ggagaagact atggcaatta
    23941 aagaaagaaa atttagggaa gtggggaact cagtagtatt aactatttca aaggagtttc
    24001 ttaaagaaaa tgggttaaat cccggagata cggttttttt agatgaagaa aaacttcgag
    24061 cggcaattac aaaaaaacaa gccttatctg atgaaattga tttagcaata tctcaagcat
    24121 taactgaata tgatactact tttagagagc ttgtcgataa atgagtattc attacctaga
    24181 agccaaagat attataaaaa tgaatgtaat ccaaataaaa aaatactctc ctaatgaacc
    24241 ccttggtgta aaggatgcga atgctttaga aatgtgtgta gggcaacttc gagcctcagc
    24301 ttttggtgaa gaggtttatc cttcgattta tgaaaaagca gctatcctac ttatacaatt
    24361 gataaagaaa catccttttc acaatgcaaa taaacgtact gctttcttag ctacttttgt
    24421 ctttttgaaa atcaatggac agcttttagc tattgatcaa aaagatgctg taaatttagc
    24481 ggtatatatt gctacgtatg ataaggattt cgatcaacta aaatatgaag tgatcaacac
    24541 tataaagaca catacgcagc ccatttaagg agttactgta tgtgtcttta tagggttatt
    24601 agctgctata gcgaaataat tgaagtaaat tacaaatagc tataattcaa atccaactat
    24661 atagaaaacg taaattttct atatagttgg attaagataa cgactattta ttttatcata
    24721 tttttactat accctatact tttacttggt atatttatga ataatcgcaa atttacccct
    24781 atactactag tttgtaagtt agattaataa aaatactaaa gttatttgtt cttttattaa
    24841 atacacctac aacttataaa ttgatggtat ttagataaca cgaaagaata aaaataacag
    24901 ctgatagtaa agtttgttga cgattgctac tcaatcgaaa gtgagttagt gcgtatgaac
    24961 ttgcttctga aaacttatga aatgaaagac cttttacatc ttgcagaaat cggtactggg
    25021 attaaactaa gcaataaaaa ctaatgttcc cctcattctt tgaacttcta ctatttgatt
    25081 tatttattct gataaatgac gatttcacct tcaggatctt caaatcccgt atagaattta
    25141 tttaagatct cttcagtatc agtaatagga taggcgatag gccacatacc ctttgatggt
    25201 tagaataaac tgctcaaaat ctgtatcgta tgttggcttt agcatattaa caaaggctat
    25261 ctccaaatta taaaccataa gcgttgaatc gttcttaaca atttcaattc ttcctaaccc
    25321 aaagtcagaa gatataatta ccggacttgc cttacccttc atgtgggttg ttgagattcc
    25381 atatttaaag ggaattgtcg gtactatcgg cggtctgaaa aacatcccaa aaataaaaaa
    25441 atctttcccc tgctagtctc ctaagtccct ttgtagcgga tatggcacca taatctcatt
    25501 ttatcgaagt gagtcatggt ttatctgttg gccaaccaaa attgatacaa tctaacgatg
    25561 caccctctac ggcattctag gggagtgcac ttgcattcac ccaataaaaa ccggcagtta
    25621 gcaaagaact tatgtagtcg tatgttctta ctgttaacat acaagaggtc tcctttccac
    25681 aattgcttag cagtaaacgg gaatttacaa agctaatctc ttccattttc ttatctttgt
    25741 tctaaaagtc ccaaatggag caactgtatt tacatgaata aatttatata cttcccatac
    25801 agccgttttg gttgcctcgt ctgcccattt tcttttgtgt gcagtgaata gatcttcatc
    25861 actcattgaa tcaatcatca tgttaatatc atcaatatta cgattgagaa tttcctctaa
    25921 ttctttcaat ggtagatgtg cataggtatt tgtaaaccac ttatataact ctcctaactg
    25981 attccattta aattgatcag aaggcgtttt aacagataat ccatttcttt catcattttc
    26041 ccattttaga attaaattcg tccaaccaac ttgatatgct aaattttcag caggtgttct
    26101 atccacttcc tcaactcttt tatccttcaa ttcttcagga atagtttcaa attctaaaat
    26161 atatttagta taagtcctat ttatttcttt ttttaattct tctttatttt tatatgttct
    26221 cattatatta cctccataaa ttcctaattg tctcctttaa ttcatctata atttcatcca
    26281 cgatatttta atccgttcta caatgcatat ctgatatgca ttgactgtct aactcatatg
    26341 tgaatgattt ataaaatatc ccaacgtctc atctaaagaa atgttgatcg tcagcaagga
    26401 gctaaaaaat acgaatatat ttcttttgta gtaaaatcgg actggtttgc atatcatttt
    26461 cttcgtaaat atcaaaatca tgttaactac tatcaattac taataaaaac taccacttta
    26521 agcgttaaga actgttaagt ttagttaagt gaggatagtt cacaaaatcc attgttaaac
    26581 ttcacgaaag tggtaaaact caaaatgctt tatataggat tacagcatcg ctctttcttc
    26641 gattgcgcgt tgggtaaaaa atattatgaa gtaaaaattg acgatgacac gttccttacg
    26701 gctcatcaga tgaaacagct acaaaaacga aatgatcaac tcgaagaaaa tttaattcta
    26761 aaaaagcgat tgccatattc acgcctcgct cgacgaacta ttaaaggtca agatttacct
    26821 gaagatcatc ctttttagga aattgaagct ttttatgttg cacaatgcat ttataatact
    26881 acaatgatgt tttcaccaga tattcttcta gggtgcggtg taatgaaaca gatacacttg
    26941 caaaagaaaa ttcaccgcga atttactaaa attatgaata attatgttaa tcttccgaaa
    27001 tttttttcca aaattaaggg atcaagctgg tgttatagat tgtttaaggt tggcaaaaaa
    27061 ataacttctt tttaaacaat tatagaaaac cgatactctg ttaagttggt tgacacggtg
    27121 tcgattttta tgactttctt attctgaaac ttcactaacg gacagtacct aaaaagtcta
    27181 tagtatggac gtttacgagt actattttat caaaaatgct gctcatttca acttatatgt
    27241 tttagtgaac tacccccact tagc
//
LOCUS       DAZUKZ010000028        26956 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000028 DAZUKZ010000000
VERSION     DAZUKZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.028"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..145)
                     /locus_tag="ACG8EF_001139"
                     /pseudo
     CDS             complement(32..145)
                     /locus_tag="ACG8EF_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(528..1760)
                     /locus_tag="ACG8EF_001140"
     CDS             complement(528..1760)
                     /locus_tag="ACG8EF_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS4946472.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2014..2583
                     /locus_tag="ACG8EF_001141"
     CDS             2014..2583
                     /locus_tag="ACG8EF_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4946473.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2761..3201)
                     /locus_tag="ACG8EF_001142"
     CDS             complement(2761..3201)
                     /locus_tag="ACG8EF_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS4946474.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3359..4123
                     /gene="map"
                     /locus_tag="ACG8EF_001143"
     CDS             3359..4123
                     /gene="map"
                     /locus_tag="ACG8EF_001143"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS4946475.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4155..5078
                     /locus_tag="ACG8EF_001144"
     CDS             4155..5078
                     /locus_tag="ACG8EF_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFS4946476.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5154..6293
                     /locus_tag="ACG8EF_001145"
     CDS             5154..6293
                     /locus_tag="ACG8EF_001145"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFS4946477.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6286..7086
                     /locus_tag="ACG8EF_001146"
     CDS             6286..7086
                     /locus_tag="ACG8EF_001146"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4946478.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7086..7913
                     /locus_tag="ACG8EF_001147"
     CDS             7086..7913
                     /locus_tag="ACG8EF_001147"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4946479.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7918..8625
                     /locus_tag="ACG8EF_001148"
     CDS             7918..8625
                     /locus_tag="ACG8EF_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4946480.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8725..9591
                     /gene="rfbA"
                     /locus_tag="ACG8EF_001149"
     CDS             8725..9591
                     /gene="rfbA"
                     /locus_tag="ACG8EF_001149"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS4946481.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9605..10177
                     /gene="rfbC"
                     /locus_tag="ACG8EF_001150"
     CDS             9605..10177
                     /gene="rfbC"
                     /locus_tag="ACG8EF_001150"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFS4946482.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10199..11227
                     /gene="rfbB"
                     /locus_tag="ACG8EF_001151"
     CDS             10199..11227
                     /gene="rfbB"
                     /locus_tag="ACG8EF_001151"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFS4946483.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11325..12176
                     /gene="rfbD"
                     /locus_tag="ACG8EF_001152"
     CDS             11325..12176
                     /gene="rfbD"
                     /locus_tag="ACG8EF_001152"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFS4946484.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12211..14244
                     /locus_tag="ACG8EF_001153"
     CDS             12211..14244
                     /locus_tag="ACG8EF_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946485.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14288..15568
                     /locus_tag="ACG8EF_001154"
     CDS             14288..15568
                     /locus_tag="ACG8EF_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFS4946486.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15778..16584
                     /locus_tag="ACG8EF_001155"
     CDS             15778..16584
                     /locus_tag="ACG8EF_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4946487.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16596..17816
                     /locus_tag="ACG8EF_001156"
     CDS             16596..17816
                     /locus_tag="ACG8EF_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4946488.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17806..19392
                     /locus_tag="ACG8EF_001157"
     CDS             17806..19392
                     /locus_tag="ACG8EF_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4946489.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19431..21569
                     /locus_tag="ACG8EF_001158"
     CDS             19431..21569
                     /locus_tag="ACG8EF_001158"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4946490.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(21937..22920)
                     /locus_tag="ACG8EF_001159"
     CDS             complement(21937..22920)
                     /locus_tag="ACG8EF_001159"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4946491.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            23298..24689
                     /locus_tag="ACG8EF_001160"
     CDS             23298..24689
                     /locus_tag="ACG8EF_001160"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS4946492.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24705..25745
                     /locus_tag="ACG8EF_001161"
     CDS             24705..25745
                     /locus_tag="ACG8EF_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4946493.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25764..26849
                     /locus_tag="ACG8EF_001162"
     CDS             25764..26849
                     /locus_tag="ACG8EF_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFS4946494.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
BASE COUNT         8620 a         4498 c         5482 g         8356 t
ORIGIN      
        1 gtatcctttg cggtacaggg ggttattttg tataagtaga cattgtagtt tctaaactgc
       61 tatgtcctaa acgtttcgat acgctaagta tatttactcc ttgataaagt aaaatagatg
      121 catgcgtatg tcttagtcca tcaataaaca gtcgaagaga aaatagggaa aaattgatta
      181 ctaaagaaaa acttactagg tcgacggtgt agttgatttt catacttcaa tagattcgaa
      241 ttaaacgatt tcgattggct tataccagtc ctatccaacg aaagatatat cgttcttaac
      301 tctagcggta taacttgaag ctttacgtaa agccaatggg aatgaactgg agatgacttt
      361 ttattatttt tagtaatagc tgatgggtta atacttacaa ctttcctttt caaagtacca
      421 tataaaaaaa cggtggggtt gtttagcgaa ataaaggatc taagccaata gcttagatcc
      481 tttattctat gctttatatt attttttgca tgtgtgcgaa agtatactca agcccaatcg
      541 ccatttcgga aaatcggtac tgtagagcca tcttcacgga ttccgtcgat atccatcttg
      601 tcagatccta ccatgaaatc tacgtgggtt tgacttctat tcaatccagc ttctgctaat
      661 tcttcatcgg acatttctgt accgcctttc acactgaagg cataagcaga acctagtgct
      721 aagtgattcg atgcgttttc atcaaataat gtattgaaaa agatgatgcc agattgagaa
      781 ataggtgaag ggtcaggtac aagtgcgact tctcctaaac gacgcgcacc ttcatctgta
      841 tcaagcagtt ttgctaagac atcttctcct tgttcggcag aaaaatcaac aacttttcca
      901 tctttaaaag taaatttcat acccgagatg atggtgccag cataactcag tggctttgta
      961 cttgaaatgt aaccatctac tcgatgactg tcaggtgctg tgaacacttc ttccgtcggc
     1021 atatttgcca tgaatttttc tccacgagca ttatagcttc cagcgccttc ccaaagatgg
     1081 tttttaggca atccgataat gatatctgta ccgggagcag tatagtgaag ggcagaaaat
     1141 tgttctcgat tcaattcttc tgcttttttt gctaattttt catcatgctt cttccatgct
     1201 aagacagggt cttcttcata gacacgagtc gttttgaaaa tctgatccca taatgcctca
     1261 acttgttttt cttctggcag ttctgggaag acttttgctg cccattgttt accagccgca
     1321 gccacgacag tccaactgac tttatttgct tgagttgcct tgcgtaaatt cattagtgct
     1381 ttgccattcg ctgattgata agacgctact cgatggctat ctacaccagc aaatgcatcg
     1441 ggatcggcag aaacaacgct gatcctgctt gctccttttt caagccattc atccgcttga
     1501 tcgattttag attgtggtac attctcaata cgatccgttg cagcatgtaa aagaaattct
     1561 ctttggatct ggtcgtcagt ccattgaacg ataacctcag cggctcctag tttataagct
     1621 tcttgtgtga tcaatcgagc aaggggtgcc tgttccacgc tgatctgtaa aacaactgtg
     1681 tgtccttttt ctgtggctac gccagtttct gcaattaagc gtgcatattt ttttagaagt
     1741 tcattaaaat cagataacat aataaaaatt cctcctcacc attttttctg gtaactatta
     1801 gcatgatacc atttaattat gattcaaaca atgaggcttt aaataaaaaa aggaggttat
     1861 ttttttaagg ttaagtttca tgatatatgt tactacttgc tatttcagct tgtgaagaaa
     1921 atcaaaaact aattttgcat atcaaaaaaa catgttacaa tgaaattaat caatggtgga
     1981 agcgttataa aaaaattaag gagattaaaa aatatggcaa gaaaaaagac gattactaga
     2041 gaacagattt tggcagctgc ttatgaagta gctgccaccg atggatttac acgatttact
     2101 gcgagaaaca tcgcgaacaa aatgaattgt tccacgcaac cgatttatct tgagtttaaa
     2161 aatatggatg acttgagaca agctcttttt gagcagatct atgatcattt acaatatgaa
     2221 gtgttcactg ttgagcatac agggaatacg attatcgatc ttgcttttaa ttatatccat
     2281 tttgcaagaa gagagaagaa gttgtatcgt tcactgtact tagaagaata tggtggagga
     2341 aaagagatgc agaatttctc ttatacttac tttgtgaata cagtgaaacg cgatccagct
     2401 tatgtgaatc tttcagaaga gcagatcgaa tctttgtata atgggacatg gatcatagct
     2461 actgggatcg cagctttgat gtcttcaagc atcatccatc caactgatga acagattgaa
     2521 aagatgatcc aagacagtat agatgctatc ttgaaattca aagaaccaat cgaaatcgat
     2581 tgaatatact caaaagttcc acaaatcagt aggtaacaag agatgtgtca aaataaggaa
     2641 aagttcgttt ttcttacagc gtatgcattt ttaactcccg aagaactctt ccatgaatgc
     2701 aatgaataaa agagccatac agattgaata aatctggatg gctcttttat tgcaagtata
     2761 ttaaattttt gcgactagtt ctttagcgaa agcttctaaa ttttcgatat cttcttcttc
     2821 agcattcaga tccactttaa cattttcggc tccttttgat gcgcctgttt ttgcaaaagc
     2881 agcatcgaaa tcatccaccg atttgcagaa ttcatcgtaa aaagtatcac cagatccgca
     2941 gacaccgtaa atttttccca atagatccaa ttcttgcaag tcttcataaa agtctacgat
     3001 ctcatcaggc agatcgccat catcgtatgt ataagtagct actacgcaaa tatctgcctc
     3061 ctcgaattca tctgcatcta cttgggtaca ttcgttgatt tcgacttcga tatccagatt
     3121 ttcaaatgct tctgcaacaa tatcggcgat ttcttcagtg tttcctgtca tgctagcata
     3181 tacgattttt gctaaagcca tgatgtgcct cctcgtttat aattatttaa caattatgaa
     3241 ttataccaaa acatcattga aaagaaaata aaacctaaag acacctgggg aatgtcattt
     3301 ggctaaatgt atgccgctat gttaaaatga gagggatttg taaaaaggag acgaaaatat
     3361 gattacttta aaatcagcac gtgaaattga aatgatggca gaatctggag ctttattagc
     3421 agatgtacat aaaaatctgc gtgattttat aaaaccagga atcacaagtt gggatattga
     3481 ggtatttgtc agaaactata tcgagagtca tggcggaatt gctgcgcaaa ttggctttga
     3541 agggtatgaa tatgcgactt gtatcagtat aaatgatgaa atttgtcatg gattccctcg
     3601 taaaaagcct ttgaaaaacg gtgatttggt aaaagtcgat atgtgtatcg atttgaaagg
     3661 cggcatttct gattcttgtt gggcctatgt agttggagaa tctacacctg agatcgaccg
     3721 attgatggaa gtaacgaaga aagctttgta catcggtatt gagcaagcac aagtagggaa
     3781 tcggattggt gacatcggtc atgctatcca aacttatgta gaaggcgaaa atctagcgat
     3841 cgttcgtgat tttattggtc atggcgttgg tcctacgatt catgaagaac cagcagttcc
     3901 tcattacgga gaagctggaa aagggttgcg gttgaaagaa ggcatggtta tcacgatcga
     3961 acctatggtt aataccggaa cttggaaaat gaaaatggat cctaacggct ggactgcgta
     4021 tacaagagac ggcggacttt cttgccaata tgaacataca ttggctatta ctaaagatgg
     4081 tccaagaatt ttgacatcac aaggagaaga aggcacttat tgatctatcc agtcaatagg
     4141 tacgggagag gtacatgaaa gtttggaaca aaataaaaca aaatgaaaat ctgatgcgct
     4201 ttattgagac gactcaaaag cgcatcactg attcagaaat gagtacgacc tctgtcgtcg
     4261 ttgcttatta tttactcttg tctttatttc cattgattat tgcttttggt aatattttac
     4321 cgtttctcca catcgatcaa gagactgtac tgacctattt acgagaagtg attcctgaga
     4381 ccgtttacca gtttatcggt cctgcaatca aagacctttt gacacaaagt tcaggaggct
     4441 tgttatccgt ttctgcatta gcggctttat ggtctgcaag tcaaagtatc aatgctttgc
     4501 aaattgcgat gaataaagcg tatggcgtag aaaatagaaa aaattttttg atcgtccgtt
     4561 tcttctcact aattgtcatt gtcttgttca tgattgctat tagtggggta actatcgttc
     4621 ttgggttggg acagctgatt ttggatgcta ttcagccaat cgtacaaata cccgtgaact
     4681 ttatcgatca gttccaaact ttgaagtggc caatcacatt ggtagcttta tttgtcatca
     4741 tgttcttgat ttatttaatt gtccctaatg ctcagttgaa gttgaagacg atcattccgg
     4801 gcacgatctt cgctactgtc ggctggatgc tgttatcaca agtgtttagt atctatgcta
     4861 ggtattttgc aacgaggatc agcggttatc agatcatagg aagttttatt gtactcatgc
     4921 tttggcttaa ttttgctgct acgatcatca ttttgggcgg aatcatcaat gcagttgttc
     4981 aagaatacgt gacgggtcat caaatcaaaa aacgtcgaga tccaagccga aaatggttca
     5041 ataaattgaa aactaaattt tctcataaaa aaaagtagaa acaagagttc ttattgtaaa
     5101 tttatatcag taatttataa taattatgac tcttaattaa agtaggtgcc catatgcttg
     5161 tttcttttag tatggttata agattttttc tgacaatttt attatcactg atattaacac
     5221 ctgtattcaa aatcatttca gtacaaacag gtatggtcga caaaccaaat gaaagaagaa
     5281 taaataaagt tccaatgcct tcagcgggcg gactgcctat ctttatttca tttgttatat
     5341 cgactttatt cttattccgt aaaattatcc ctcaatcgta tattgtaccg atcttgattg
     5401 cttctttagt aatcatagtc acaggagtgc tcgatgataa atatgagctg acacctaaac
     5461 aaaaaagtat agggatcttg attgcttctc ttattattta ttttgtagca gatatacgta
     5521 ttgattcctt cacgcttcct tttattggat atatccaact aggctggctt agttttcccg
     5581 taacgatttt ttggatcttc ggtatcacga atgcggtcaa tctgattgac ggtttggatg
     5641 ggctggcggc agggatttca ttgatcggtt taattacgat aggaatcatt ggatattttt
     5701 tcctccatgc ttctacagtg tatattccag ttgttatttt ctgtcttgta gcaagtatta
     5761 tcggtttttt tccttacaat ttttatccgg caaagattta tttaggcgat acaggagctc
     5821 tattcctagg gtttatgatg gccgtccttt cactacaagg gctgaagaac gtgacttttg
     5881 tttcatcgat ttctttgctg atcgtcatgg gagtgccggt aacagacacg ttttttgcta
     5941 tcattaggag aaaagcaaac cgtgtttctt tttctacagc agataaaaaa catcttcatc
     6001 accgactaat cagtttaggc tttactcata aaggtgctgt cctgacgatt tatgcgatgg
     6061 ctttgatgtt ttctttcaca gctatggtca tgaattatac tggaagaatc ggaacgatcg
     6121 tattgattat tgccatgttg tttgctgcaa ttttattatt tgaattgatt ggtctgatca
     6181 atgagaaaca tcagtttatt ttaaaaacat tgcgattttt agggaataaa gagtaccgta
     6241 cgcaagtaaa acagaaatat ttgaagaaat ggaaccgtca tatagatgaa taacaaatta
     6301 aaccagccga tccttattag tattgtgacg gcaaacagtg ataaaatttt tcggacacta
     6361 gataatttta tttcaactgt aaaagatgaa acagcagtaa aaatccgaat ttttgacaac
     6421 aattcacttc ccgagtacaa aaaaagacta acagagtata ggaaatatcc ctacatcgat
     6481 atctattttc atgatgaaaa ttccggattt ggttatggac ataatcacaa tctgatagct
     6541 agcgattctc gctatggcat tatttgtaac ccagatattt tagtggatga agagacagtt
     6601 acagcattgg tcttgttatt gaaagaacat ccggaatgtg cgatgttagc acctaagatt
     6661 cttaatgaag atggaacaac tcagcacctc atccgtgagc agctggctgt ttttgattac
     6721 gttttgcgct ttgtgccttt caaatgggtc aaaaaaaagt ttgacaaacg actggctgtg
     6781 tttgaatgcc gaggattgcc tactgatgct aatagttatg tacggatgat ctcaggtagt
     6841 tttatggtag ctgatattga taaatttaaa gagataaagg ggtttgatga gcgtttcttc
     6901 atgtactttg aagacaacga cttgtgtttg agttttgaaa aagcaggtta caagttattg
     6961 tacacaccct tttatgcagt tatccatttt tacgggaagg gtgcgcatcg aaatgcaaaa
     7021 ctgtttcgcg tgtttttgac ttctatgatg aaatttttca ataagtgggg gtggcgtttt
     7081 ttctgatgaa accagaaata ttagtagtcg ttgtgcttta tcagcaacta ttcagtcaat
     7141 ctccagctta tggaccatta agcaaagcac ttagcgaaaa gcaaattcag cttattattt
     7201 acgataatag tcctcaaagg cagcaaaatg agctgtttga aaaagaaaat atcgtttatc
     7261 atcatgatcc gcaaaatcct ggtttagcgg tggcttataa ttttgcgttg tctcaagcag
     7321 atgaaggaac acgttgtctc gtcactctgg atcaggatac gaggctagtc gacgattatt
     7381 ttgaactatt gcgtaaagta acctttacag atgaatgtgt tgctgcagtt ccgatgattt
     7441 tttctgggag ccgacaagtt tctcccctat attcagatcg ttatatcaat cgtacagctc
     7501 aagcagtaga agttgaaggc ccaacagctg aacgaatcat ggcaatcaat tcaggtgttg
     7561 cctggtctgt caaatttcta aaaaagattg gcgggtttaa tcctgctttt tctttagatt
     7621 ttttggatca ttggctgttt tggaaagtgg atcagttgga aaaaactgtg gagatccttc
     7681 ctgtacgaat ggaacatgat ctttcggttt tagactatga aaaagtttca agtaatcgat
     7741 accgttctat tttacaagca gaaaatcgat tttatcatga atatgatcaa aggcatttat
     7801 ccagtcaccg acgacaatta ttgttaagaa cagctaaaca gttcttgacc gtcaaaaacc
     7861 ggcatatctg gcggatgact ttgcgctcat atgttaagaa ttggaaggtg tgaattcgtg
     7921 cgttcggttt gtatagctac ctttaatgga ggcagatatt taagagagca gttagatagt
     7981 attttatctc agctttcagc agaagatgaa gtagtcattt ctgatgatgg gtcagtagat
     8041 gatacatggg atatattaac tgaatacgca gaaaaagata agcgaatcca gcttttttcg
     8101 gggcctcgtc aaggattgat cgctaatttt ggttatgcga tccagcagac gaaaggagat
     8161 ctaatctttt tggcagacca agatgatgtt tggttggaga caaaagtgaa aaagatcact
     8221 gactattttg agcagaatcc ggatcagcta gtagtagttt ctgatttaat cattgtggac
     8281 agcgagctgc aagagatcca tgattcgtat ttttcgtttc gaaattccaa agaaggatgg
     8341 cttaataatg tgataagaag tcactatatt ggtgctggta tggctttccg gtcttcttta
     8401 aaacccgata ttttgccgat cccgtcagaa gtgccgatgc atgatatgtg gattggtttg
     8461 ctgggaggaa agaagactgg gttcctaaga gaaccgttaa ctctgtaccg aagacatgat
     8521 ttaaacgcga gtgagattga aacacattct aaattacggc aaaaaatttc ttggcgtgtt
     8581 catctgatta cggcgttgat caaacgaaaa tggtttaaac gttgataaaa gatgtttaaa
     8641 tccttaatat ttccatgaca aatataacaa ttgatgagat aataacaaag tgaaatttga
     8701 aatcaaaaag gagagcagat atccatgaag ggaatcattc ttgctggggg cagcggaaca
     8761 cgtctgtacc ctttaacaaa ggcaacatcg aaacaattga tgccgattta tgacaaacca
     8821 atgatttatt atccaatgtc tactctgatg ttggcgggaa tcaaagaaat tctgattatt
     8881 tcaacgccac aagatacacc acgtttcaag gaattatttg gaaacggaaa tgatttagga
     8941 ctgcagatcg aatatgcagt acaagaaagt ccagatggat tggcacaagc gtttatcatt
     9001 ggagaagaat ttattggaga tgatagtgtc tgcttggtat taggggacaa tatttactat
     9061 ggtggcggtc tgtctaaaat gttgcaacga gcagcttcaa aagatacagg cgcgacagta
     9121 tttggctatc atgtcaatga tccagaacgc tttggtgtag tagaatttga tgagaatatg
     9181 caagctttgt cgattgaaga aaagcctgct cagccaaaat caaattacgc tgtaactggc
     9241 ctttattttt atgacaatga ggtaatttct atcgctaaag gtatcaaacc ttcagaacgt
     9301 ggagaactag agattacgga tgtcaataaa gcatatttgg ataaaggaaa actatccgta
     9361 gaacttatgg gacgcggatt tgcttggctt gatacaggaa cacatgaatc attgcttgaa
     9421 gcatctacct ttatcgaaac aattgaaaaa cgtcagaact taaaagtggc ttgtttggaa
     9481 gaaattgctt accgtatggg ctacatcgat aaggaacaat tgctggccct tgcacagcca
     9541 ttgaaaaaaa atcaatacgg tcaatattta ttgaatttgg ccgctgaata aggagtcctt
     9601 acagatgaaa gtaacagata caaaattaac agatgtcaaa atcgtagaaa tggacgtctt
     9661 tggcgatcat cgcggtttct tcacagaaag ttattcaaaa gcaaaatttg cagaacatgg
     9721 attgaaatat gactttgttc aagacaacca ttcactatca gctgaagctg gtgtccttcg
     9781 ggggcttcat ttccaaaaag gagaagctgc acagacaaaa ttgattcggg tagtcactgg
     9841 agcagtttta gatgtagtag tggatatccg caaaggtagc ccgacttatg gtcaatggga
     9901 cggatttatc ttatctgaac ataatcatcg acagttgctt gtacctaaag gatatgccca
     9961 tggattcgtt actttgacac caaatgtgaa cttcctttat aagtgtgata attactataa
    10021 tgcagaagca gatgggggca tcgcatttga tgatccagat ttagcgattg attggccaat
    10081 tgatccatca agagcgatta tgtcggaaaa agatcaccgt catccgacat taaaagaatt
    10141 tgaagcagaa aatccatttg tttacggaga aatctaagaa ttaaggagaa attttcttat
    10201 gaaaaacatc attgttaccg gaggtgccgg attcatcggc tctaatttcg ttcattacgt
    10261 ggtaaacaat catccagaag tgcatgtcac tgttttggat aaactgactt atgcaggaaa
    10321 caaagagaac ttggccggac ttcctagcga tcgcgtagaa ttggtcgtag gagatgttgc
    10381 agatgcagaa ttagtggacc gtctagtaaa agaagcggac gcagttgttc attacgcggc
    10441 agaatcccat aacgataatt cattaaaaga tcctttccca tttgtacaaa cgaatatcat
    10501 tggtacgtat acacttttag aagcttgccg caagtatgat gtacgctatc atcacgtatc
    10561 tactgacgag gtttacggcg atcttccatt acgtgaagac ctgccaggac atggggaagg
    10621 cgaaggagaa aaattcactg cggaaacacc atataatcct tcaagtccat attcttctac
    10681 aaaagctggt tcagatctgt tagtaaaagc atgggtgcgt tcttttggct tacgtgcaac
    10741 catttcaaac tgttcaaaca actatggtcc ttatcagcat attgaaaaat ttattcctcg
    10801 ccaagtaacg agtatcttaa gcggtattcg tccaaaatta tatggcgaag gaaagaatgt
    10861 tcgtgactgg atccatacga atgatcattc ttcagcagtg tggctgattt tgacgaaagg
    10921 cagaattggc gaaacctatc tgatcggtgc agacggtgaa gaagataata agaccgtgat
    10981 ggaattgatc ttagaaatga tgggccaacc aaaagacgca tacgaccatg taaacgaccg
    11041 cgcagggcac gatttgcgtt atgcaatcga ttcgacaaaa ttgcgagaag agctaggttg
    11101 gcagcctgag tttacgaact tccgtgatgg gttggctgac acgatcaaat ggtacgaaga
    11161 acatgaagac tggtggaaaa aagaaaaaga ggccgttgaa gcagcatatg ccaaaaatgg
    11221 acaataatac agaggaaatt tcactgtatt aatggaaaaa cacatgtgag gcaggcgctc
    11281 gcctgtctct tttttaaaat aaaaatcaag gagcggataa ccatatgttt ttattaactg
    11341 gaggaaatgg acaattaggt acagaattgc gccatttatt agatgaaaaa aatctgccat
    11401 atgtttcaac agatgcaaaa gacttggata tcacagatgc tgagaaaaca atggcttata
    11461 ttacagaatt aaaacccgat gttattttcc attgcgcagc ttacacagca gtagataaag
    11521 cagaagaaga agcaaaagaa ttagatgaga aaatcaatgt cgatggcaca agaaatgtag
    11581 cacttgcagc aaaagctgtg aatgcacaat tcgtctatat cagtacagac tatgttttcg
    11641 atggaaaaaa caaaaatgta gaatacgaag tagacgacca aacgaatcca ctaaatgaat
    11701 atggtcgtac gaaattatta ggcgaaaaag cagtagaaga gattttagaa gactattata
    11761 tcatacgaac ttcttgggtt ttcgggatct atggacacaa tttcgtcttc acgatgcaac
    11821 gcctagcgga aacccgcgac aaactgactg tagtaaatga ccagtttggg cgaccaactt
    11881 ggacacgtac tttggcagaa tttatggtat atgtcatcga agaaaaagca ccatttggta
    11941 tttatcattt gtcgaacgaa aatagctgtt cttggtatga gttcgcaaaa gaaatattga
    12001 aagatacaga tgtagaagtt gcacctgtta cgtcagaaga atttccgcaa aaagcaacac
    12061 gtccgcagta ttcagtcatg agcctgaaaa agacagaagc gttaggtttt gtcataccaa
    12121 cttggcaaga agcacttgcg caaatgctgg aagctgccaa aaaaatgaat aagtaatttc
    12181 ctttttatca aatagaattt ggagcatgat atggaaaaac aaacaagaag tgcacgacga
    12241 aaagaaaagc caaaagcgaa tcaggcagat tcgaatttgg tgcttgacct gtttctaaaa
    12301 ctccgctatc ttattggcgt tttagtcatc gtggtcatcg ttacttttaa tttaaacgga
    12361 agttcacttg gtgtttggga taaatatgtg tcgcaaagag acgatgggaa aaaaactgat
    12421 gtgatttttg gacagaatcg ggaagtgcgg tcagacgaat ggctggtgca gacacctttt
    12481 tacctctcac aagctgaaga ggacttttcg ttggtgaacg aggattattc attaaacgga
    12541 caaaacatga tcattgcgta taattctcct gtaaaggata ttacggttat cggaaaacca
    12601 ttcaactggg gattcttttt cctcggaaga gatcgaggac tttcctttta ttgggcgatg
    12661 aaattagtcg tcatggtctt gcttggattc gaagtactga tgatattaac gaagaggaaa
    12721 aaagctctat cgctaatcgg tgcatttgcg ctgacatttt ctccagcagt ccagtggtgg
    12781 ttcatgcaac atgtgggcga tttgattttc tttactttgg gactgatgat tgctttttat
    12841 cattatttct acaatcatga taaaaaatgg ctacgaacgt tgatgatgct tttggtggtt
    12901 attttcggta ttgggtttat tttagtgatt tacccagccc atcaagtgat gctggcgtac
    12961 ttgctgattt tttattttgt tggcctgctt atttactata gcaagaaagt ttcatgggat
    13021 ctgttggatg tagtgttgat tggaggggca gttttattta ttggcggagt catggttcat
    13081 ttctggctaa cgtcaaaaga cgcattgtta gcttctttga ataccttata tccaggaaaa
    13141 cgggtaagta ctggaggaaa gtggacgatt ggcgaatttt tttctttcct tacaaattgg
    13201 aaaattcctt ttgaagatat caactattcg aataactctg aagtggccct gttctatcac
    13261 ttttttccaa ctgtattcct agcaagtcca tttgtgttat ttggcaaaaa agatagcgaa
    13321 caaaagttac tgggacgtat tttgatgatt ttttgtctgt tttctatctt ttggattacc
    13381 gttgggttgc caaaatggct agcagaaatc actctgctgt cttatgttcc tccattaaga
    13441 gccatcctta cttttagttt tgcggcttgt ttattatcga tctggtttat cgcttatatt
    13501 tggagagaag atgttattcc tatttggtat aaattcattt tgttggcagt aaatgcatgg
    13561 tcttatttct atgcgattac gcattcgaaa atggataatt atttttcgga ttttgaaacc
    13621 atcgcagttg ctgtgttggg aacagtgatt ttagcttttg cattatttgg ctggagacgc
    13681 ttgttttacc tcgcttttgt cgccttagtt atcgtatctg gctttttcgt caatccagtc
    13741 gtagaaggta caggagcgat ttttgaaaaa acactggcaa aagaaatcca aaaaatcgat
    13801 caacaagatc ctggtaaagt ttggttgtct gaagatgaat tatataactt caccccaaca
    13861 ttgggagtga aatccttcaa tacagtacgc ttttatccag atatagctgc atggaagaaa
    13921 atcgatccga aagaagaata cgagaagtat tataatcggt atgctcatac aagagctttt
    13981 atcgctaagg aagatacaaa gttcatttta gatcgtccag atatgttttc ggtcacgttg
    14041 aattttgaag acgcagaaaa attagggatc gactatgtga tcagtaaacg accattaact
    14101 gattacaacc aattatatga agcgcagttc acgcagctat atggaccgga taaagacggt
    14161 tatatgattt ttaaagtaga atatccggat tatccagatg taccacaaac acaaactcag
    14221 gaaaatcagc tagtcgtaca gtaataggct agctgagaat aatcctgaaa tattagtaga
    14281 aaggagagtg aagataattc aagcaatcgt acaaaaattt gtcaacaaaa tgagtgtcgg
    14341 tttcttgctt ttgttgctga cgctagtctt ttttgtttgg acagcagggg taggttttcc
    14401 agcaactttg tttatatata acaatagttc attacttatc gcttttagtg tggctatttt
    14461 gctcatttta aatgtttata aagcaaaagg cgtcgatttt ctttatctag gtgttgcgct
    14521 tgcccttttc attttctttt cttactttcg ggaattaaga aatgagtctt tatttggaga
    14581 cttactgttt cctttggtta ttttattttg tttagtaatt aaatggatcg agtggacaaa
    14641 atgggatcgc ggtatttatc tgattgtctt tacggtcttc ctcggaatca cgttatatcg
    14701 tgtatttaca gagatccaag taccagaagg acaaagcatc tgggctccag gcaattcgct
    14761 ttctgatatt tggatcaata cgaatacaat cggaagttct ttgatgactc ttgctttatt
    14821 gatcagtgga tttgccagtt cttttgaacg atggtatgtt cgaatcatcg gtgggatagc
    14881 agtcgttgca gcctttttag gtatttgggt ctgccaatct agaggtgcat tagtcgcgtt
    14941 gattgttttt gcgatattgg atcttttacc taaatcgttg atgcgtgtca ttcgtgcgcc
    15001 atttattgcc tacacggtta cgattctttt agctttgccg atttcttatc tggcggctgt
    15061 ctctgaaaaa gtcaatttgt ttaccgatcg agaagatatt tggcataaat tctatcagac
    15121 gctaggagaa aaatctgaac aagttttact aggaatgaaa acatttatct tccagcgagg
    15181 aaaccaattt ttaggaaacc acaatagtta taattctatt ttgaatatct atggattgat
    15241 aggatttggg attgctgctt tgctgcttat cctttttatt ggtcgcttaa cgttgaaagc
    15301 agatttatcc aacgggcaga tgacttttat ctgggccttt tttgcggtga tgatgcagtc
    15361 atttatggag gatacattga cttcataccc atgggttccg attatttata tcctgttggc
    15421 tatggccgtt catcgatatg atgaaccaga agttgcggag gaattctatg ttatggaaga
    15481 ggcatatgac gatatgactt atgatgatac atcttatgag gaacaaccag atagcgctca
    15541 ttcttccagg atccaacgct atcattgata ggcacaaaat acgagagagg ctgtgacaac
    15601 cgtcgcagcc ttttttggtt tattgcaatt gtctagcatc ttctgtaaaa tagaaacgtt
    15661 agagaaaatc ttgtatcata gaaaattaag aaagaaataa gaacgcagct cggtaccaaa
    15721 aatatggtaa aatggcactt gactgtaatt cactgaaaat cgcttggagg aacacaaatg
    15781 tttaaagatt tgtatttatt tatgaaggac atttatcaga acagaaaatt attgctacag
    15841 ttttcgatca atgacttcaa atcaagatat gcaggatcgt ttttaggaat cttatgggcg
    15901 tttatcaatc cgctatttac tgtattgatt tattggctgg tatttggtgt tggactgaaa
    15961 gcacaaatga tcgatgcaaa atacccattt atcgtatatt tagttaccgg gatgattccc
    16021 tggttcttct tctcagatgc atttgcatca actacggttg tttttcggga atatacttat
    16081 ttagtaaaga aagtcgtatt caatattcgt atcttgccca cgacgaaaat cctatccaat
    16141 ctgtacaccc atttattctt cattctgatt ggcatagctg tcgcgcttat aaatggtata
    16201 tatccttcag caatgtcttt acagctgatt tattatttat tttgcatgtg tgctttcttg
    16261 acaggattga cttggctgac ggcatcgatc caaccatttt tacctgatat catgcaatta
    16321 attacgatcg tgctgcagtt gatcatgtgg acattaccga tattatggag ccctaatcag
    16381 tttgattcag gtcttgtaag catattgaaa ttaaatccgc tgtactatgt tgttcaaggt
    16441 tatcgtgagt catttttaag tgaagcttgg ttctgggagc atggaacata tactttgtat
    16501 ttctgggttt tcacgttagt aatgttcgta atcggctctg cagtgttccg tagattgaaa
    16561 ccacattttt cagatgtgct gtaaggagaa tagctatgac agaatatgca attagactaa
    16621 aagatatcac aaaatcttat aatatgtacg caaaaccttc ggatcgattt cgtgaagccc
    16681 tcaatccttt taagaaatcc tatcatgatg tattttatgc gttgaaaaac gtgaatgtag
    16741 aaataaaaaa aggtgagatg atcggtttcg tgggagaaaa cggatcggga aaatctacaa
    16801 tgttgaaaat cattacggga gtactgacac caacatccgg cacgatggaa atcgaaggaa
    16861 aaatctcagc tttgctagaa ctaggatcag gattcaatcc tgagtattct ggctatgaaa
    16921 atatctattt aaatggaatg gttctaggat tttcaagaga agaagtcgat gaaatggtcg
    16981 atgatatcat tgaatttgct gacatcggag accatattta tcaacctgta aaaacttatt
    17041 caagcgggat gttcgttcgt ctagcttttg ctgtagcaat caatgtgaat cccgatattt
    17101 tgattgtgga tgaagcattg gcagtaggcg atcttgaatt tcaactaaaa tgtatggaaa
    17161 aatttacaga aatcaagaac tcgggaaaga cgattttatt cgtttctcat gatatcagtt
    17221 cgattcgtcg tttttgtgat cgttcctatt ggttgaaaaa cggagaagtc gtcgaatatg
    17281 gcgatacgat ggaagtaacg acgaattatg aaaacttttt aaagaaaaaa tcagttaaaa
    17341 cagtggatcg gaataaaaac caagtagaac ggttcgccgc tccagatatc gtcgacgtga
    17401 cgaaagcgga attgttggat aaagacaaaa atccggtgga tatgctggaa caaggagaaa
    17461 aactatacgt taaagtaact tatgaagtaa aagatgatac aatcaaaaat ccggttttag
    17521 gaattgcgct acgaacagtc gacaattttt atgtatgcgg acttaatact ctactagatg
    17581 gtatcaagat tccttggaaa aaaggagaga atatctttta tctggagtat gaaaagttag
    17641 gtttgttagc tggtgaatat tattttgacg ttgcggtctt tgaagaaaat gcaacggttc
    17701 ctttggtata taagacaaag tacatgaact tgtttgtcag cggttcttat atcggagaag
    17761 gcatcgtggt cttagaccat aaatgggaag aagggactca tagcaatgaa atatgatttt
    17821 gaaatggacc tagatgaaca aagcagtgtg ggaaaaattg ccgcacagat caaaccagga
    17881 agtaaagttt tagagtttgg tcccggaaac ggccgattaa cgaaacattt aatcggagca
    17941 aaacaatgtg aagtgagcat tgttgagtta gataaggaat tgttcgattt tgtcagcgaa
    18001 tttgctcaag atggatttta tggtgatatc gaaagttttg agtgggcaaa ctattatgct
    18061 ggacagacat ttgattacgt cttgtttgcg gatgttttag aacatttggt agatcctgga
    18121 aaaacattga agaaagtcag agaatttttg aatgaagaag gagagatcct gattacattc
    18181 cctaacttgg cgcataattc tgtcatgatc gatttgttca ataaccaatt gccgtgggct
    18241 tcttatggtc tcctagatga aacccataat tctttttata ctcatgatgg cttccaaaaa
    18301 gtttttgaaa aagcgggatt attcattaat attgaagatt atttgtactt agctgtaggt
    18361 gatacagaat taaaatcaac ttatgaagaa ttgccggaag ctgtacgcta tgatttcaaa
    18421 atgcgaccat ttggtgaagt ctaccaatac tttttctcat tgatgaaaca tccagtagca
    18481 caaagttcca ttgcagagcc acaaaattct aattatgtaa aagtgttaga agtaacccag
    18541 caaacgaaac aagatgaaac aatccaacaa ataccattca ataatttcac aggggaaaat
    18601 gagacactaa gtttccccgt atcagaaaca acagaaagaa tggtgtttcg cttcgcgcaa
    18661 caacctagct ttatcgaatt cagcgcagaa gctgccggag aaaaactaac atttatcgat
    18721 tcgaatgctg tagtcaaaac agtaaatgat tgttatttgt ttgatgggaa agaattacca
    18781 gagttcgttc taacggatat ttctgggaaa gaagtgacta tccattgtca ctatcgcttt
    18841 atcggggaat tgacaccaac aatgaaagaa ttgttggaaa cgattcgtcc catggctgaa
    18901 gtcacaaagc agctttctga gaaaaatgac gaattagaaa gagaaaatca tcatcttctt
    18961 gaggaaaata caagactaga ccatacgtta aaaacaacca caaatcgcta ttgcacattg
    19021 ttggaaagtg atgaatggac aataaaacat agactaggac gacgtaagaa agaaacttcg
    19081 aaaaaaatcc aagaaaaaga actttctata tgtattgacg agaaaatctg ggatgcagaa
    19141 acaaaaatat taaaaatcat cggatgggga atcgctaatt cggatcgcca gcccttgtcc
    19201 tataagttat cagctgacca atcgccattt ttcgaagcaa ttcctgtttc acgagaagaa
    19261 gtcaatcaag cagaacagct cgcaaaagga acagaagcgg gatttgagtt acgaatcctt
    19321 tgcgagcaag aacgttcatt cttagtggaa gcagtagctc aaaatggcca atcatggatc
    19381 attgaaatgt aaataatgat taacttataa agataaggag taaacgaatc gtgcaaaaaa
    19441 aagaaattgc cgtatttatc gatcaaatca ctcgtgaacg ggccactggc gatttgctta
    19501 ttgtgggctg ggcaattgat gaagtgacca aagaaatccc tacgataaag gtagaaaaag
    19561 aaaatgtgat tgctgaagca acacatgtcg taagattgga tatcaatcat ttatataatc
    19621 ttgatgttaa gacccaaagc ggtttcaagg tacgtttatc agggaaaatg agaggaaaag
    19681 ccatcttaga cttccaaaca gcgagacatc aaaatggcat agctgtgaaa ttaaacggta
    19741 aataccctta tgatgacggc attgaatctt catgggaaag aaaaaaaaga ttactgaaaa
    19801 aagggataaa ttatgcaagg actcatggcg taaaaaaagc gatccgtcgg gtcaaattgg
    19861 aattgaagcc aggatcgatc gactatgcag agtggatcag tcgtcatgag aaaccagatt
    19921 tgaaagagca aaggaaagaa gcacaaggat ttgcgtaccg tccattgatt tccatactta
    19981 tgcctgtcta taacgttgaa atcaaatggc tcgaaaaatg cattgattcg gtactggatc
    20041 aaacgtatga tcattgggaa ctatgtatca gtgatgatgc ttctactgat ccagctatcc
    20101 gaaagtgtct ggaatcctat caagcaaaag atgatagaat caaagtcgtt ttccgaaaag
    20161 aaaatggaca catcagtctg gcaactaatt ctgcattgga aatagcagaa ggagaattta
    20221 tagcactctt agataatgat gatgaattac cgccatttgc attatatgaa gtggcaaaag
    20281 tgctaaacgt acatccagaa ctggatgtga tctacagtga cgaagataaa atcgatgcag
    20341 atggcaatcg ctttgatccg catttcaaag cagactggtc gcctgataca ttgatgggaa
    20401 ataattatat ctctcactta ggcgtatatc gaacaagcat tgttaaagaa cttggcggtt
    20461 tccgtaaagg ctatgaaggt tctcaagatt atgatttggt ccttcgtgtg acagaacaaa
    20521 tccctgcaga tcatatttac cacattgacc gagtcttgta tcattggcgg acgatcccag
    20581 gatcaactgc aagtaatggc gaagccaaaa gttatatcta tgattccgga gtgaaagcat
    20641 tgacagatgc cctgtctaga agaaacatca aaggaagcgt acgtcctggc agaatttctg
    20701 gattttatga gattgcttat gacgtcttgc aagaggattt ggtcagtgtg atcattccaa
    20761 cgaagaatgg ctatgaagat ctaaaaactt gtgtggattc gatcatcgaa aaaacgtctt
    20821 attcaaatta tgaaatcatt attgcggata acggaagtac agatccaaaa atgcaagaat
    20881 tatttgcaga atacaagcat cagctgaaag accgctttat cgtagaattg attgacattc
    20941 cattcaatta ttctcgaatt aacaatttag cagcagaaaa agcaaacggc aaatactttt
    21001 tattcttgaa taacgataca gaagtgattg agccagattg gatgacggtg atggtttctt
    21061 atgcacagtt tgatcgaatc ggctgtgtag gagcaaaact gttttatcca gatgatacaa
    21121 cgcagcatgc aggagtatta ttaggaatcg gtggtgttgc agggcatgca ttgaataact
    21181 atgatcgaac acattgtggt tatttcggcc gattggtcat tgacgtcaat tacttagcag
    21241 tcactgcagc ttgtatgatg gtcaaggcag ctgacttcaa tgctgttaac gggtttgatg
    21301 aaacattaga agtagcattc aacgatgttg acttgtgctt gaaagtttat gaactgggtc
    21361 gttacaatgt ctatgcacat caagcagaat tgtaccattt cgaatctaaa tcaagaggtt
    21421 atgaagatac tccagaaaaa caaagacgtt ttgctggtga aatcaaaaaa atgcaagata
    21481 aatggccagc atatattgcg catgatccat tttacaatga taatttaaca aaagacggaa
    21541 tcggcgactt ttcgttgaga ccagattaaa ctataataaa aagctgttat gttgtgattc
    21601 gctcgcacat aacggctttt tatagttttt tgtcaaaaaa aggcagaagg tgccgactca
    21661 aaaaaataag tcgaggttgg gacagaagtg tttaactccg agaaataaga agaaattcac
    21721 gaaaattgct tttcaaattt ttgtgaattt cggcttattt aagaagaagt tgcttctgct
    21781 cccaccgttt attcgttttt aaagaatgag acaaaagtgt ttcttacttt tgtcccacgc
    21841 tcttattcat gctcagagcg ccaatagata caaaatcata gccaaagttt cacgaaccct
    21901 agctgtcaaa gaatcgatat tttattttaa tataggctat tttaaaataa atggtttaag
    21961 gtattcctct acaaaagcca ctctatcaat tatcatgggt ttaaaataag aagtgatgga
    22021 gataaccaga ttgttcttag cgtcaatata aagaacattt ccactattac caattgctga
    22081 atatatttcg ttctcagaat ctaataccca ccataaatag ccatatctca tattttgtaa
    22141 gtcttctaaa gcactggttc taggtttgaa catctcgttt atccacatgg gtggtacgat
    22201 ttgttttcct ttatacatcc ctttatttag gcataataca ccaatttttg ccagatcact
    22261 tgccgataaa cacaaaccat atcctgccgt cccgatattt agtggatcac aaaaccagac
    22321 attggtttta ggagacttat ccattgtaaa ctgcttatgt tcttcagctg tttcagcata
    22381 ataattgaca tggtttggta tccctatagg atcaaataga tacttattgg caaaatcaac
    22441 agttgttagc ttacttgtct gatacaacaa gccagttaat atgtgtaaac agacagtttg
    22501 atagttgaat tcgccggtta taccttttct tcctcccaat aaatctagtg atgtttttgt
    22561 ccaatcttca cttgaacaga cttttgtcca tggatcgcct ttacatttgt agggtgcttt
    22621 cattgttagc aagtgcttga ttgtaatagc gtaaatagtt ttctccccgc gtttcacttt
    22681 atactctgga aaataatcta atactttatc atcgatactt ttgattaatc cttgctctaa
    22741 agcaatgcca atcagcaaag atacaatact tttcgttgct gacatgatat gacaagtatc
    22801 gtctttttta taaccattcc attcatctgt ataaacttct accccatcct tatatgcgac
    22861 aatttgacat atatttggtt gtcgaaaacc aataaaatca tgcaactctt gtttattcat
    22921 aagatattac ctcactttct tctataagca taatgtcatt aaaaatcgat ttcaattttt
    22981 tcgattcttc acgtaataca ggtattctta tggactagac agcgctttgg gaagatggat
    23041 tctctgaaaa atataaaggt tcactttatg agtggataaa aaaagcagca aatacttcga
    23101 cttctttaat ttaccaacat tagtgaaaat cgattcatct ggagatttgt aatttagagt
    23161 ccttgtcttg attagaggtg tataaagctt tttattagac atttttaaat tccattacaa
    23221 tttgcttata aactaggttt ttaaaggtga ctatggtaag ataatagaga tattattttg
    23281 tgattaggag tgtatatatg aataaaaatg gagaatggag tgctgcacga cggatattca
    23341 ttatattaat ggatgttatt gtatacaatg cagcaatata tagtagtttc ttactgaagt
    23401 ttcgtggaga gatcccatta cgaaatttag aaactttcca acactcagca attattattt
    23461 cagtgatttt tattgcgttg aacattttgt tgggtgcata tgttttttat aatcgaatga
    23521 tcagtgacat cgtgtttgtc acagtcatcg gtcaggtatt gatgacacta gggatcatgg
    23581 tagtgacttt tgcgggccgc tggtttgctt ttccacgtct tgttatctta ctttcatttg
    23641 ttctcggtac gcttttctta agtatctatc gtattctcgt atataaactt tatttacgcg
    23701 taagtcgaga taaaaaggtc gtcattattg ggttagaaaa ggatgtcgca ccagcaatcc
    23761 aaaattttgg ttcaaagaaa aacaacaaac ataaagtcga agcagtcatt cttgataact
    23821 attatgcaaa catcaaaaat atgattaaag acatcgatat tgtctatctt gcttctcata
    23881 tcgaagaaac tgaaaaaatc aaaatctatc aattagttac taaaaatgaa aaaaaattgt
    23941 ttttgaatac aacatttgaa aacttgacat tgatcaatcc aaatattatg aatttcgaag
    24001 atgaaagtat tattgaagca tctggattta gaatccaacc tgaatatgaa ctattcaaac
    24061 gtttgtttga tatcatcgtt tcacttgttt tattagttat tgcttcacca tttatgttat
    24121 tcacagcaat tttagtcaaa gtgacatcac cgggaccaat catctataaa caagtgcgga
    24181 tcacaaaaaa tcaaaaggaa ttttcaatct ataaattccg ctcgatgacc gccacagccg
    24241 aagcaaaatc tggaccagtt ttagctaaga gcaatgatgc acgagtaacg cctgtaggta
    24301 aatttattcg agctgtccgc tttgatgaat taccacaaat cttcaatgtg ctgaaaggcg
    24361 atatgtcgat cgtaggacca cgtcctgaac gttctttctt cgtcgatcag ttcaatgcag
    24421 aaaaccctta ttactactta cggcataatg ttcgtgctgg tatcactggg tatgcacaag
    24481 tctatggaaa atatgtctct gattacaaca gtaaattacg ttttgattta ctttatatca
    24541 aaaaatattc attgatgctt gatttaaaga tcttgctgca aaccatcaag atcctatttg
    24601 ataaaatgtc ctctcaagga cttgaagaag acgaaaatgt gatctcattg gcagaaattg
    24661 cttttccgaa tgaaaaaatc tttcgctagt tgggaggcgt aaaaatgaac aatccgaaat
    24721 tcagcataat cgtaccagta tacaatgcag aagcaactat tggtcgaacg attgatattc
    24781 taaaagaatt ggattatgaa gagtacgaag tcgttttagt aaatgatgga tcaacagatc
    24841 aaacacagga aatcattcaa gaactgattg aaaatgatga acgttttgaa ttgattacta
    24901 cgaccaatca agggcccgga ttagcaagaa acgaaggaat aagacagtca aaaggagaat
    24961 atctcttatt cttcgatgca gatgacacgc cagtaaaaac gatcttgtca gattatgatc
    25021 atttgctaga aaagagtcca aaagcggatc ttgttgttag ttcgtttgtt tttcgtacaa
    25081 tggacgaggg aagaatcgtt tccgaaaaag aaaacttagt tacagaatgt cactatctaa
    25141 ctcacgaaga gtttataaaa gacatgtatc agttaatgaa tgaacagttg atgtatgttg
    25201 tatggaacaa atgctatcgt agcgatattc tgaaaaataa ccaaattcta tttaaaactt
    25261 ataatagttg tgaggatcga atatttaact tacattatta tgaatactgt catcaagtga
    25321 tcatgaaccc aaaagtagaa tatatttatg aatttgaagg tgggaagggg atcactaatc
    25381 aataccgacc taacaagttc gaaacattta aagaattcta ttcattagca aatactgtaa
    25441 cagacgatat taataaagac ggtatggcgg cgcttctttt aaaagggaca acttcagtta
    25501 ttttttctat ttatagtacc tctgcaagaa cttcaaaaga gaagaaagac gaagcaaagc
    25561 agatattgag tgatccagtg atccgagaag ccaagcaaat tgctaagacg gattcttcag
    25621 caaaaaaaat aacaaagttg ctttacaata tgccagctcc actattttta tctgctgtaa
    25681 aagttggaag cttcgttgaa gtgaaaatgc cagggttaat ggctgctttg aagcgagtat
    25741 attagttaat ggaggaaaat ataatgaaaa gaattttgca ttttcaggga cgaatgggtt
    25801 taggtggagc agagtctttc atgatgaatt tatatcgtaa aattgatcgt acaaactatc
    25861 aatttgattt tttgatttat gaagattatg cagatgttca agactatcat tcagagattg
    25921 aaagattagg aggccgtatt tttgttgtac ctaatcctaa gaaaaatatt tttaaatatt
    25981 taatagaagt gaataagtta ctaaaaaaag aatctttttc tatagttcac aatcaggttt
    26041 attttggcgg gggaattaat ttatggttag ccaaaaagaa tggaatcaga cagagaattg
    26101 ctcatagcca tgcaactgaa gatggaaaat ctcaaaatat tgtaatgaat gtattgcgta
    26161 aatttttaac caaattgttg ttacaaaatg caacagacta ccttgccgtt agtcaagaag
    26221 ctggtgaaag tctgtttcaa aaccatccct ttgagatagt tcataatggt attgatttag
    26281 agctttatag taaaaattca gaagctaagg ttaataagag aaaagaattg gatatctcaa
    26341 tgagtacttt tgttgtggga aacattggac gattagagaa acaaaaaaat caatcttatt
    26401 tgttagagat ttttttagaa atactaaaaa tgaattctga ttcttacttg attttagtag
    26461 gtgaagggtc acttagaaat aaactgaaag ccaaagcagt agaattggat atagagaaaa
    26521 gagtaaaatt tttaggagaa agaaacgata ttcccgagtt attatctgtg atggatgttt
    26581 ttgtaatgcc ttctctttat gaaggtctgc caatggttgg attagaagca caagcagcaa
    26641 aattaaaatt agtcttagca gatactattt ccaaagatac aaagttaaca aataatgtgg
    26701 attttgtatc attagatgaa acaccacaag aatgggcaaa aaaagtaata aaagagccgt
    26761 atgctaataa aataacacaa gaactaaaga gttatgatgt ttcgtacact actgaacaaa
    26821 tggagagtat atatagtaga gatcattaaa aatgttcaag atattttagg agtgattatt
    26881 tttgaaaata tgttatttga tattggcata caatgatatt ggcaacttaa agagattggt
    26941 caatcgattg aacgaa
//
LOCUS       DAZUKZ010000029        24650 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000029 DAZUKZ010000000
VERSION     DAZUKZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.029"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..81
                     /locus_tag="ACG8EF_001163"
     tRNA            8..81
                     /locus_tag="ACG8EF_001163"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="ACG8EF_001164"
     tRNA            101..173
                     /locus_tag="ACG8EF_001164"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="ACG8EF_001165"
     tRNA            201..274
                     /locus_tag="ACG8EF_001165"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="ACG8EF_001166"
     tRNA            288..361
                     /locus_tag="ACG8EF_001166"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="ACG8EF_001167"
     tRNA            385..474
                     /locus_tag="ACG8EF_001167"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="ACG8EF_001168"
     tRNA            487..560
                     /locus_tag="ACG8EF_001168"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="ACG8EF_001169"
     tRNA            579..654
                     /locus_tag="ACG8EF_001169"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="ACG8EF_001170"
     tRNA            656..726
                     /locus_tag="ACG8EF_001170"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="ACG8EF_001171"
     tRNA            740..813
                     /locus_tag="ACG8EF_001171"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="ACG8EF_001172"
     tRNA            838..925
                     /locus_tag="ACG8EF_001172"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="ACG8EF_001173"
     tRNA            947..1018
                     /locus_tag="ACG8EF_001173"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="ACG8EF_001174"
     tRNA            1041..1130
                     /locus_tag="ACG8EF_001174"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="ACG8EF_001175"
     tRNA            1143..1216
                     /locus_tag="ACG8EF_001175"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="ACG8EF_001176"
     tRNA            1235..1307
                     /locus_tag="ACG8EF_001176"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="ACG8EF_001177"
     tRNA            1311..1383
                     /locus_tag="ACG8EF_001177"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="ACG8EF_001178"
     tRNA            1388..1458
                     /locus_tag="ACG8EF_001178"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="ACG8EF_001179"
     tRNA            1471..1544
                     /locus_tag="ACG8EF_001179"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="ACG8EF_001180"
     tRNA            1556..1627
                     /locus_tag="ACG8EF_001180"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     regulatory      1816..1895
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1893..3086
                     /gene="metK"
                     /locus_tag="ACG8EF_001181"
     CDS             1893..3086
                     /gene="metK"
                     /locus_tag="ACG8EF_001181"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFS4946495.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="ACG8EF_001182"
     CDS             3216..4688
                     /locus_tag="ACG8EF_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS4946496.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="ACG8EF_001183"
     CDS             4771..5559
                     /locus_tag="ACG8EF_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4946497.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="ACG8EF_001184"
     CDS             complement(5626..6294)
                     /locus_tag="ACG8EF_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFS4946498.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="ACG8EF_001185"
     CDS             6409..8121
                     /locus_tag="ACG8EF_001185"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4946499.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9893
                     /locus_tag="ACG8EF_001186"
     CDS             8124..9893
                     /locus_tag="ACG8EF_001186"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4946500.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10098..10664)
                     /locus_tag="ACG8EF_001187"
     CDS             complement(10098..10664)
                     /locus_tag="ACG8EF_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4946501.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(10664..11620)
                     /locus_tag="ACG8EF_001188"
     CDS             complement(10664..11620)
                     /locus_tag="ACG8EF_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFS4946502.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(11620..12276)
                     /locus_tag="ACG8EF_001189"
     CDS             complement(11620..12276)
                     /locus_tag="ACG8EF_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS4946503.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            12646..14664
                     /locus_tag="ACG8EF_001190"
     CDS             12646..14664
                     /locus_tag="ACG8EF_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4946504.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    14734..14940
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15091..17505
                     /gene="leuS"
                     /locus_tag="ACG8EF_001191"
     CDS             15091..17505
                     /gene="leuS"
                     /locus_tag="ACG8EF_001191"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFS4946505.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            17853..19406
                     /locus_tag="ACG8EF_001192"
     CDS             17853..19406
                     /locus_tag="ACG8EF_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFS4946506.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(19787..21571)
                     /locus_tag="ACG8EF_001193"
     CDS             complement(19787..21571)
                     /locus_tag="ACG8EF_001193"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4946507.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(21586..23307)
                     /locus_tag="ACG8EF_001194"
     CDS             complement(21586..23307)
                     /locus_tag="ACG8EF_001194"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4946508.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            23407..24168
                     /locus_tag="ACG8EF_001195"
     CDS             23407..24168
                     /locus_tag="ACG8EF_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4946509.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7678 a         4499 c         5198 g         7275 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa taaagaggtt
     9901 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag cttctcatgt
     9961 ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt gaataaagag
    10021 gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac atatcattta
    10081 tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg cggctggttc
    10141 ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag tttggaacaa
    10201 aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta gacgacaagg
    10261 atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt gcgtgtagtc
    10321 tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt gaaaatccct
    10381 gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc ttgtagaaac
    10441 aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc atcgattgct
    10501 tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc tgctagaaaa
    10561 accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg ttgcaagatt
    10621 tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg ctcacctttt
    10681 tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca tcaattgcat
    10741 tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc cacggcgcat
    10801 cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg tcgcaaatga
    10861 cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag tcccgcaaca
    10921 ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata tctgaatcta
    10981 aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt aatccgttga
    11041 tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca tctaaataag
    11101 tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt gtttgcaatc
    11161 caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca actacttcat
    11221 ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt aaattgacta
    11281 cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta aaattttgga
    11341 aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg atttctttct
    11401 ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa ccagatacac
    11461 tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag tcaaatcctc
    11521 catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg taattccaag
    11581 aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct tctttctgta
    11641 agagttatct atttgttttc ttttttctct atacactgga taagtcaggc acagccaaga
    11701 aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca gataaatgcg
    11761 gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg tgcgtaaggt
    11821 tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa tcatactgcc
    11881 tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg ccaatgaagg
    11941 acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa ttacaataaa
    12001 tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc ataaagccgc
    12061 acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa agagttggta
    12121 gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat cgaaaccaga
    12181 aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga caagaaagaa
    12241 gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt gaagcaatga
    12301 cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac caatgtatga
    12361 ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga aagacatttt
    12421 ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa tccttttaaa
    12481 caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt acggtaaatt
    12541 acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat attaagagaa
    12601 gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga aaacattgca
    12661 agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa acgaggggca
    12721 gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt acagccggtt
    12781 cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac agcggcattt
    12841 atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct atatgcatca
    12901 gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt atctcaagca
    12961 ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt ctacatggat
    13021 acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg acaatatcct
    13081 tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc atttggcaac
    13141 ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga tgcgacagct
    13201 tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa taatatcatc
    13261 gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg cggtggagcg
    13321 actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag caattctggt
    13381 aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg tgattctgtt
    13441 tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg gaacaacatc
    13501 caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc
    13561 tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg tacatcaaac
    13621 ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt ttggaagatc
    13681 tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat caagaacaat
    13741 ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa tgctggagca
    13801 aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc aagttcttct
    13861 aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg gagcgtctca
    13921 aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca aaataacttt
    13981 atctacccag gacaacaact gactgtttca aacagtggat caacaaacag cacgtcaggt
    14041 aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg cagcagttcg
    14101 tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac
    14161 ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt catttatcca
    14221 ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg ttcaactact
    14281 tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc atccactggt
    14341 aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa caaacatcat
    14401 atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat ctatccagga
    14461 cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc agttactggt
    14521 tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga cagtcatgga
    14581 attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat ttatccaggt
    14641 caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt gaattccaca
    14701 aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa
    14761 aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc ttttgaactc
    14821 gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg gccgttacaa
    14881 cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta cgtcctataa
    14941 aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag aaaacaaagc
    15001 aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat aatagaaaca
    15061 agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga aattgagaaa
    15121 aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga tcctgaaaag
    15181 ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct acatgtaggg
    15241 catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag tcaaggatac
    15301 aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca atatgcgcta
    15361 gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt ccgacgccaa
    15421 atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac tgatccggag
    15481 tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct agcttatgaa
    15541 gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa tgaagaagtg
    15601 atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat gcgccaatgg
    15661 atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt agttgattgg
    15721 ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg agcaaatatt
    15781 gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg accagatact
    15841 ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag agaaattacg
    15901 acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa aaaatcagat
    15961 ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc ttatgcaatc
    16021 aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt atcttcatac
    16081 ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga attcgctcaa
    16141 actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa agctgcttac
    16201 acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa acaagaagca
    16261 atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga agtcagctat
    16321 cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat cccaatcatc
    16381 cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt acggttgcct
    16441 aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa tatcgaggag
    16501 tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac gaatacaatg
    16561 ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca taacaaaaaa
    16621 gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta cattggtgga
    16681 gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt cttatacgat
    16741 atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg aatgatctta
    16801 ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc tgatgatgtg
    16861 gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg gccattagat
    16921 gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt ggatcgtgta
    16981 tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac attgaatgat
    17041 ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga ctatgaaaat
    17101 cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc ctataaagtg
    17161 gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc gattgctcct
    17221 cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc ttacgcacca
    17281 tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt tttccaagta
    17341 aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga tgagctagaa
    17401 aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac agtcagaaaa
    17461 gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc tctttgtcaa
    17521 taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca ttctgtctca
    17581 gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga ttttcatgtt
    17641 ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct ctgtaatcat
    17701 caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga aattgagagt
    17761 acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga ttttgacgca
    17821 taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt attatcgaag
    17881 tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa agaataatgt
    17941 tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct gtatcgttcc
    18001 tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa ttaaataatt
    18061 tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt gtttttcttc
    18121 tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg cttagtcaaa
    18181 gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat ctttattttg
    18241 agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag ttattgctga
    18301 gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc ggaatgaaca
    18361 cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag tcttcgcctg
    18421 gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc attcttttag
    18481 ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat ataaatgctg
    18541 cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca aatgaagggt
    18601 tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat gatctcaaac
    18661 ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat tgggtatttg
    18721 tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg gggctgattc
    18781 ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca tggtttatct
    18841 attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa acatggcaaa
    18901 cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta ttttatggca
    18961 acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact ttgtacattt
    19021 acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg atcaatcagc
    19081 cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca cgtgaaagtt
    19141 tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg attccggaaa
    19201 actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact caaataagcg
    19261 cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat gcagggaaaa
    19321 tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc gttcagcaac
    19381 tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg ttcagcaccg
    19441 agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt gtaactcgtg
    19501 attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc gtattttaca
    19561 agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt aaaaagggat
    19621 tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc caaaaagtat
    19681 cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa aagctatcga
    19741 tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac gctatctgaa
    19801 attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca gaatgtgttc
    19861 ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt tcaatcgttg
    19921 acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc attgcttgct
    19981 gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa ataacgacag
    20041 gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct tgagataacg
    20101 tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga ataaagtgat
    20161 cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc attttcccgt
    20221 aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa accatcccaa
    20281 aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg aattcgatct
    20341 ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt tttcctgcac
    20401 cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg acttgattca
    20461 tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt tttcctttcg
    20521 gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca tctaatatct
    20581 caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca taagaagcgg
    20641 tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag aagccgatcg
    20701 tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg aaggcgagtt
    20761 gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca cgtttaaaag
    20821 cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct tgctgattga
    20881 atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt aaaacaccca
    20941 gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta gttgtaacaa
    21001 ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg atataaaaca
    21061 gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg atacctgtca
    21121 ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg tcacctactt
    21181 gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt tcttttcgca
    21241 ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata aatgaagtga
    21301 tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat gcttgtttga
    21361 tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag agatcatcga
    21421 tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat aaactgcata
    21481 gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa cgtatcaaag
    21541 cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata ttccttgtga
    21601 ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag taccttgtcc
    21661 aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg tactgaggcg
    21721 ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg ctcttaatgc
    21781 tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga tctctgctgg
    21841 tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat aattagaacc
    21901 gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt cttctaattg
    21961 agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc ccatctgcaa
    22021 gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac tgatgtggga
    22081 gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta cttttcctat
    22141 cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag atccggtccc
    22201 gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt ctagaactgg
    22261 atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat ttgtctgatc
    22321 aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct ctgcttgcag
    22381 tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca ataccatagc
    22441 tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag tcacgacacc
    22501 gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag aagcataaac
    22561 gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt tcactttgat
    22621 catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat atgattgatt
    22681 atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga tggtgatccg
    22741 atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca gtcgataaac
    22801 aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata gagaataacg
    22861 aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca tttggcaaaa
    22921 taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca aaagagatcc
    22981 agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga aatatttctc
    23041 ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag aggcaactgc
    23101 agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt ttataactga
    23161 ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg gtacacctaa
    23221 tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc ctttgttttt
    23281 ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat acttttcgat
    23341 tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa aaagaggtga
    23401 gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga tttgccaaaa
    23461 tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca agaagagaat
    23521 gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt tttttataga
    23581 aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt gacggagtta
    23641 tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt gatgaaacaa
    23701 aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga tacaaacgag
    23761 gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga gtttgaagat
    23821 attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat gagtatgcca
    23881 gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt acatttattt
    23941 actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt cttattgaaa
    24001 tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag aatgtatgaa
    24061 aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac ggatgaaaac
    24121 aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga aatatgtgtt
    24181 taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct ggttcgatta
    24241 cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt tttagaggaa
    24301 atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg taattgtcct
    24361 cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat ggtgaactga
    24421 gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat gagaatgata
    24481 aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac attattacaa
    24541 tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt aatcaaatag
    24601 tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt
//
LOCUS       DAZUKZ010000030        24575 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000030 DAZUKZ010000000
VERSION     DAZUKZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.030"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..550)
                     /locus_tag="ACG8EF_001196"
     CDS             complement(152..550)
                     /locus_tag="ACG8EF_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFS4946510.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="ACG8EF_001197"
     CDS             complement(547..1320)
                     /locus_tag="ACG8EF_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS4946511.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="ACG8EF_001198"
     CDS             complement(1317..2000)
                     /locus_tag="ACG8EF_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS4946512.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="ACG8EF_001199"
     CDS             complement(2167..3054)
                     /locus_tag="ACG8EF_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4946513.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="ACG8EF_001200"
     CDS             complement(3051..3920)
                     /locus_tag="ACG8EF_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4946514.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="ACG8EF_001201"
     CDS             complement(4057..5343)
                     /locus_tag="ACG8EF_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4946515.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="ACG8EF_001202"
     CDS             5616..6194
                     /locus_tag="ACG8EF_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4946516.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="ACG8EF_001203"
     CDS             6194..7123
                     /locus_tag="ACG8EF_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4946517.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="ACG8EF_001204"
     CDS             7120..8856
                     /locus_tag="ACG8EF_001204"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4946518.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACG8EF_001205"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACG8EF_001205"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFS4946519.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="ACG8EF_001206"
     CDS             complement(9752..10261)
                     /locus_tag="ACG8EF_001206"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFS4946520.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACG8EF_001207"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACG8EF_001207"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFS4946521.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="ACG8EF_001208"
     CDS             complement(11562..12653)
                     /locus_tag="ACG8EF_001208"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFS4946522.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACG8EF_001209"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACG8EF_001209"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFS4946523.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACG8EF_001210"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACG8EF_001210"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFS4946524.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACG8EF_001211"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACG8EF_001211"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFS4946525.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="ACG8EF_001212"
     CDS             complement(16616..17590)
                     /locus_tag="ACG8EF_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFS4946526.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="ACG8EF_001213"
     CDS             complement(17571..19454)
                     /locus_tag="ACG8EF_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFS4946527.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="ACG8EF_001214"
     CDS             complement(19472..21697)
                     /locus_tag="ACG8EF_001214"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS4946528.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="ACG8EF_001215"
     CDS             21812..22846
                     /locus_tag="ACG8EF_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4946529.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="ACG8EF_001216"
     CDS             complement(22913..23926)
                     /locus_tag="ACG8EF_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4946530.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="ACG8EF_001217"
     CDS             complement(24004..24192)
                     /locus_tag="ACG8EF_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFS4946531.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7061 a         5341 c         4397 g         7776 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc ccaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24541 tattggcaaa tcgccaaaat aatgtagact aacta
//
LOCUS       DAZUKZ010000031        24355 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000031 DAZUKZ010000000
VERSION     DAZUKZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.031"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG8EF_001218"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG8EF_001218"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFS4946532.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACG8EF_001219"
     CDS             complement(1172..1867)
                     /locus_tag="ACG8EF_001219"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFS4946533.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACG8EF_001220"
     CDS             complement(1880..2596)
                     /locus_tag="ACG8EF_001220"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFS4946534.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ACG8EF_001221"
     CDS             2926..3534
                     /locus_tag="ACG8EF_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFS4946535.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ACG8EF_001222"
     CDS             3859..4182
                     /locus_tag="ACG8EF_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFS4946536.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ACG8EF_001223"
     CDS             4364..5734
                     /locus_tag="ACG8EF_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFS4946537.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ACG8EF_001224"
     CDS             complement(5864..7165)
                     /locus_tag="ACG8EF_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS4946538.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="ACG8EF_001225"
     CDS             complement(7519..9510)
                     /locus_tag="ACG8EF_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFS4946539.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ACG8EF_001226"
     CDS             complement(9654..9920)
                     /locus_tag="ACG8EF_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4946540.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACG8EF_001227"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACG8EF_001227"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFS4946541.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACG8EF_001228"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACG8EF_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFS4946542.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /gene="def"
                     /locus_tag="ACG8EF_001229"
     CDS             13264..13827
                     /gene="def"
                     /locus_tag="ACG8EF_001229"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS4946543.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ACG8EF_001230"
     CDS             complement(14080..14601)
                     /locus_tag="ACG8EF_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFS4946544.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ACG8EF_001231"
     CDS             complement(14604..15446)
                     /locus_tag="ACG8EF_001231"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFS4946545.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ACG8EF_001232"
     CDS             complement(15439..16164)
                     /locus_tag="ACG8EF_001232"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFS4946546.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ACG8EF_001233"
     CDS             16340..16636
                     /locus_tag="ACG8EF_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946547.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16892..17066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17204..18796
                     /locus_tag="ACG8EF_001234"
     CDS             17204..18796
                     /locus_tag="ACG8EF_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4946548.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ACG8EF_001235"
     CDS             18809..19549
                     /locus_tag="ACG8EF_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4946549.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="ACG8EF_001236"
     CDS             19717..21066
                     /locus_tag="ACG8EF_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4946550.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="ACG8EF_001237"
     CDS             complement(21194..21784)
                     /locus_tag="ACG8EF_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS4946551.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            22020..23099
                     /locus_tag="ACG8EF_001238"
     CDS             22020..23099
                     /locus_tag="ACG8EF_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4946552.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(23655..24029)
                     /locus_tag="ACG8EF_001239"
     CDS             complement(23655..24029)
                     /locus_tag="ACG8EF_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4946553.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(24052..>24355)
                     /locus_tag="ACG8EF_001240"
     CDS             complement(24052..>24355)
                     /locus_tag="ACG8EF_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HFS4946554.1"
                     /translation="EGYEFRISLINNQLTFGKTPNYPWPQMFDKGLQRPLFLEIGKEY
                     ELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGAIKVDKLDYSINYECGF"
BASE COUNT         7029 a         5028 c         4662 g         7636 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aaaggcggat tttataatta acttagccat ttggaaataa aaaaacgcca
    21961 cgtgaattcc atgttatatt gaagttacca caactctcaa tagacaagga tgaattcaca
    22021 tgacgcaaaa ccatgataac acagtttcac gcaagggaaa acacctaacc tactcagaac
    22081 gttgccaaat cgccatcctg aaaaaggaaa actattccaa ccgtcaggtt gcggatgtcc
    22141 tgggctgcgt gccgcagacc ataaacaacg aaatcaaacg cgggaccgtt actcagctta
    22201 accgccagaa tcagaacggg aaggtctacg actattatca tgacgtctac gacgccgatg
    22261 ccgggcaggc ggcctacgag aagaaccgct tgaactgcgg cagacggccc ttatgggccg
    22321 acacggacgc cttcattgaa tgggcggacg acatgatgct gttggaaaaa tggtccccgg
    22381 acgtggtggt gggctttgcc agaaaacacg aattgttcga cccggccatt atccctggga
    22441 ccaccaccct ctatcagtgg atcgaccgtg gcatcatgaa aacgaagaat atggacctgc
    22501 tagaaaaact gtcccgcaaa ccgaaatcgt cccgtcatgg caaccgcccg aataaacggg
    22561 tgctagggaa atccattgaa gagcgcccgg aggcggttgg tacccggaaa accttcggcc
    22621 attgggaagt cgacacggtc gtaggaaaca agaccaagtc ggatgccgtg ctgctgacgt
    22681 tggtggaacg gcagacacgc ttcgaggtca ttctgaagct ggccgggaag gatgccgaat
    22741 cggtcaacca agcggtccga cagctgagag aacgggctgg tgcgtacttc ggccagctgt
    22801 tcaagacggt tacctcggac aacggctcgg agttcgcggg actgcacgat gccttgcagg
    22861 atgtggcaaa catctacttc agccacccct atgcttcctg ggagcgcggg acgagcgaaa
    22921 accaacacaa gctgattcgc cgtttcatcc ccaagggcag cccaatcggc aacgtgagcg
    22981 aaaaccaaat cttgcggatt cagcgttgga tgaacgatta cccgcgaaag attttgggct
    23041 atgcaacgcc gcatgacacg ttcgtccagg catttaggca ggaacggttg acggcttgac
    23101 acgtgggccc ttgacaacga gcctccctcg gcatggcttg tgtctggaaa gctggccggg
    23161 gccagccagt cgggagacta ccatgcctca gctcattgtc aagggcccac tcgattagaa
    23221 aaattagacg gttggtggta acgagcaacc gggtcaccat gggtggctaa cttaaacttg
    23281 aaatttacga aaataaaaaa aggcatacag acaataaact gtctatacgc ctcacagact
    23341 ctattctata catctaattt tggctgtcct gcaaaaaata tgtgttagcc tccatcgaaa
    23401 gatcaaactt tggaaaacat cattataact ttgcttggta agagctttgc taaaacatga
    23461 tatgacttgt atacaaaatg tggagtgtat tccaataaat aggaaggatt acaaaatttg
    23521 aaaccaaata agtaaaaaat actgtcactt tgaattaatt tgaaatagta aattctcatt
    23581 aaactattga aatgctgata ccaaaaaatg aagtactttt cgttcatacc aacttagagc
    23641 acttcgttat ctatttattt cttcatttgg cttatctcta taatctcatg atttggtccg
    23701 tagaaattaa agtaacggat tccattttcc cagaacggta attccataat atttttatct
    23761 aataattcat atcctaaatc ttgaattgaa ttgaatacct catctatgtc atccgtatct
    23821 aatgctagat gatcaatggc tcctctacac tcagtagttt tatcttccca tatttctatt
    23881 atcgttccat tcaactcgaa aaagccgact ttacttccca cattcatttc ttcattaatc
    23941 agactaaacc ctaaggattc ataaaagatt tttgaagctc caaaatcttt tgttggaatt
    24001 ccaatatgtt gtaaaccatt taatttcact tacatcatcc cttcaattgt tttaaaatcc
    24061 acattcataa tttattgaat agtctagttt atctaccttg attgctccct ccgtaacagt
    24121 catacttaat gctgttccga ctttatcata cattctagta cttaaagcaa cattatcaat
    24181 atataaaaca gctatcgtat catcaacaat taactttagt tcatattctt tgcctatttc
    24241 caaaaatagt ggtcgttgca gccccttatc aaacatttga ggccaaggat agttcggagt
    24301 ttttccgaaa gtaagctgat tattaatcaa tgaaatacga aattcgtatc cttct
//
LOCUS       DAZUKZ010000032        23885 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000032 DAZUKZ010000000
VERSION     DAZUKZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.032"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..789)
                     /locus_tag="ACG8EF_001241"
     CDS             complement(55..789)
                     /locus_tag="ACG8EF_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS4946555.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ACG8EF_001242"
     CDS             1116..1526
                     /locus_tag="ACG8EF_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFS4946556.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ACG8EF_001243"
     CDS             1519..2220
                     /locus_tag="ACG8EF_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFS4946557.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ACG8EF_001244"
     CDS             2220..3806
                     /locus_tag="ACG8EF_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4946558.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ACG8EF_001245"
     CDS             3825..4046
                     /locus_tag="ACG8EF_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFS4946559.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ACG8EF_001246"
     CDS             4043..4804
                     /locus_tag="ACG8EF_001246"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFS4946560.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ACG8EF_001247"
     CDS             5184..5693
                     /locus_tag="ACG8EF_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS4946561.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ACG8EF_001248"
     CDS             complement(5768..6307)
                     /locus_tag="ACG8EF_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFS4946562.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACG8EF_001249"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACG8EF_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFS4946563.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ACG8EF_001250"
     CDS             7320..8171
                     /locus_tag="ACG8EF_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS4946564.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ACG8EF_001251"
     CDS             8199..8834
                     /locus_tag="ACG8EF_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFS4946565.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ACG8EF_001252"
     CDS             8854..9627
                     /locus_tag="ACG8EF_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4946566.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ACG8EF_001253"
     CDS             10035..10832
                     /locus_tag="ACG8EF_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFS4946567.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ACG8EF_001254"
     CDS             10810..11223
                     /locus_tag="ACG8EF_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4946568.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ACG8EF_001255"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ACG8EF_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS4946569.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ACG8EF_001256"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ACG8EF_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFS4946570.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="ACG8EF_001257"
     CDS             16000..16560
                     /locus_tag="ACG8EF_001257"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFS4946571.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACG8EF_001258"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACG8EF_001258"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFS4946572.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ACG8EF_001259"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ACG8EF_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFS4946573.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ACG8EF_001260"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ACG8EF_001260"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFS4946574.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG8EF_001261"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG8EF_001261"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFS4946575.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG8EF_001262"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG8EF_001262"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFS4946576.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACG8EF_001263"
     CDS             21907..22485
                     /locus_tag="ACG8EF_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4946577.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACG8EF_001264"
     CDS             22537..23019
                     /locus_tag="ACG8EF_001264"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFS4946578.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACG8EF_001265"
     CDS             23136..23510
                     /locus_tag="ACG8EF_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946579.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7810 a         4289 c         5123 g         6663 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttgagagtat aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg
    23881 aagtc
//
LOCUS       DAZUKZ010000033        22644 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000033 DAZUKZ010000000
VERSION     DAZUKZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.033"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACG8EF_001266"
     CDS             133..3660
                     /locus_tag="ACG8EF_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFS4946580.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACG8EF_001267"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACG8EF_001267"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFS4946581.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACG8EF_001268"
     CDS             7448..7789
                     /locus_tag="ACG8EF_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS4946582.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8EF_001269"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8EF_001269"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFS4946583.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8EF_001270"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8EF_001270"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFS4946584.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8EF_001271"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8EF_001271"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFS4946585.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACG8EF_001272"
     CDS             13000..13737
                     /locus_tag="ACG8EF_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4946586.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACG8EF_001273"
     CDS             13751..14566
                     /locus_tag="ACG8EF_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4946587.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACG8EF_001274"
     CDS             14579..15226
                     /locus_tag="ACG8EF_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4946588.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACG8EF_001275"
     CDS             15242..15898
                     /locus_tag="ACG8EF_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4946589.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACG8EF_001276"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACG8EF_001276"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFS4946590.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACG8EF_001277"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACG8EF_001277"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFS4946591.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACG8EF_001278"
     CDS             18240..18758
                     /locus_tag="ACG8EF_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4946592.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8EF_001279"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8EF_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFS4946593.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ACG8EF_001280"
     CDS             complement(19645..20565)
                     /locus_tag="ACG8EF_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFS4946594.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20691..21743
                     /locus_tag="ACG8EF_001281"
     CDS             20691..21743
                     /locus_tag="ACG8EF_001281"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFS4946595.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACG8EF_001282"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACG8EF_001282"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFS4946596.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         7539 a         3949 c         4823 g         6333 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atag
//
LOCUS       DAZUKZ010000034        22182 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000034 DAZUKZ010000000
VERSION     DAZUKZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.034"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..1948
                     /locus_tag="ACG8EF_001283"
     CDS             50..1948
                     /locus_tag="ACG8EF_001283"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFS4946597.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2247..2966)
                     /locus_tag="ACG8EF_001284"
     CDS             complement(2247..2966)
                     /locus_tag="ACG8EF_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFS4946598.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    3156..3203
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            3303..3635
                     /locus_tag="ACG8EF_001285"
     CDS             3303..3635
                     /locus_tag="ACG8EF_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946599.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3622..5625
                     /locus_tag="ACG8EF_001286"
     CDS             3622..5625
                     /locus_tag="ACG8EF_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFS4946600.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5644..6114
                     /locus_tag="ACG8EF_001287"
     CDS             5644..6114
                     /locus_tag="ACG8EF_001287"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFS4946601.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6135..6719
                     /locus_tag="ACG8EF_001288"
     CDS             6135..6719
                     /locus_tag="ACG8EF_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFS4946602.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6734..7720
                     /locus_tag="ACG8EF_001289"
     CDS             6734..7720
                     /locus_tag="ACG8EF_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFS4946603.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            7710..8021
                     /locus_tag="ACG8EF_001290"
     CDS             7710..8021
                     /locus_tag="ACG8EF_001290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFS4946604.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8043..9824
                     /locus_tag="ACG8EF_001291"
     CDS             8043..9824
                     /locus_tag="ACG8EF_001291"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFS4946605.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9817..11193
                     /locus_tag="ACG8EF_001292"
     CDS             9817..11193
                     /locus_tag="ACG8EF_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFS4946606.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11200..11835
                     /locus_tag="ACG8EF_001293"
     CDS             11200..11835
                     /locus_tag="ACG8EF_001293"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFS4946607.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11865..13220
                     /locus_tag="ACG8EF_001294"
     CDS             11865..13220
                     /locus_tag="ACG8EF_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS4946608.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13412..14806)
                     /locus_tag="ACG8EF_001295"
     CDS             complement(13412..14806)
                     /locus_tag="ACG8EF_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS4946609.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            14898..15869
                     /locus_tag="ACG8EF_001296"
     CDS             14898..15869
                     /locus_tag="ACG8EF_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFS4946610.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15866..16648
                     /locus_tag="ACG8EF_001297"
     CDS             15866..16648
                     /locus_tag="ACG8EF_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFS4946611.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16817..17188)
                     /locus_tag="ACG8EF_001298"
     CDS             complement(16817..17188)
                     /locus_tag="ACG8EF_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4946612.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17322..17765
                     /locus_tag="ACG8EF_001299"
     CDS             17322..17765
                     /locus_tag="ACG8EF_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS4946613.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17808..18617)
                     /gene="yidA"
                     /locus_tag="ACG8EF_001300"
     CDS             complement(17808..18617)
                     /gene="yidA"
                     /locus_tag="ACG8EF_001300"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFS4946614.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18636..20003)
                     /locus_tag="ACG8EF_001301"
     CDS             complement(18636..20003)
                     /locus_tag="ACG8EF_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4946615.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(19987..20817)
                     /locus_tag="ACG8EF_001302"
     CDS             complement(19987..20817)
                     /locus_tag="ACG8EF_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFS4946616.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            20980..21417
                     /locus_tag="ACG8EF_001303"
     CDS             20980..21417
                     /locus_tag="ACG8EF_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFS4946617.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21493..22113
                     /gene="rpoE"
                     /locus_tag="ACG8EF_001304"
     CDS             21493..22113
                     /gene="rpoE"
                     /locus_tag="ACG8EF_001304"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFS4946618.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT         7114 a         3850 c         4782 g         6436 t
ORIGIN      
        1 gcataacaac tttataatgt tgagtatacc aatttttgga ggcgttttaa tggaccgcta
       61 tttagattta ctttcagaga agttccctac aacggaagat gtcatcacgg aaatcatcaa
      121 tctcgaagca attttgcagc ttccaaaagg aacagagtta tttttgagcg atatacatgg
      181 cgaatttcct gcttttgatc atattctgcg tatcggttca ggaaatctta aagaaaagat
      241 caaagacctt ttctctgaac aaatgacgga aacggagatc agccaattca ctatttttac
      301 ggcttatcca gaatatgtgt tgacgactga ttggtacaaa aaacaagata aatcccagct
      361 gatccatcgt ttgatcgatc ttttacggtt tacgacagtc aaatacactc gatcaaaagt
      421 tcgaaaagga ttgccgaaag aatatagcta tatcattgag gaactcttgt atatcgatga
      481 tcgtatcaac gggaaaaaag actatgtaaa aaaaatcatt gaacaacttg tcttgatgaa
      541 ggaagaagaa aaatttttaa caaaacttgc gcagacgatc caaaaatctg tcatcgatca
      601 tttgcatatc gtgggtgata tttttgatcg aggaactcaa gcagccaaag taatggatcg
      661 aatcatggat ttttcctctg tagatatcca gtgggggaat catgatattt tgtggattgg
      721 tgcgtattgc ggctctgagg catgtttatt gacgctttta cggattgccg cacgctacaa
      781 ctatttattt gaattagaga aagaatatgg attaaattta cgtccattat tttcttttgc
      841 ccatcaaacc tatcagaaga atccagtatt tacccctaaa aacaggaaag atctatctga
      901 aagagagcaa gaagaattag aaaagatcca tcaagcactt tctatcctcc aattcaaatc
      961 agaacatcag ttgttgaatc gacgacctga atttaaaatg gacgatcgta aattgttgga
     1021 aaaaatcaac tatgaggaat caacgatcgt tttagaaggt cagctctatg agttgaaagg
     1081 gacttgtttt caaacgatcc agccagatga tcctaaaaaa ttcttggaag aggaacaaaa
     1141 agtgatcgat agtttgatgt attcattcca gcactcgtta agaatgcaaa aacacatgac
     1201 gtttttgatc gaaaaagggg gaatgtatct cacgaacaat cagcatgtac tgttccatgg
     1261 ctgtatacct gtagatgaaa aggggcagct cttagcattt gaattagacc aatcttatcg
     1321 cggaaaagaa ctgctgggat tttttgaaaa acagataact gaaagcgcaa aagatttgac
     1381 tgcaaatgaa gatttggcga ccgatttgat ttggtatgcg tggagcgggc cattttcgcc
     1441 tttattcggc aagagtaaaa tggcaacttt tgagcgctat tttctcacag agcctcatac
     1501 gcacgtcgaa aaaagcaatc cctattatca cttgcgagat gaagagtggt tcagtgaaaa
     1561 aattttggca gaattcgatg caaaagaaga aggttcagca atcatcaatg gacacacgcc
     1621 agtaaaagta aaaaaaggag agtcaccgat caaagcaaac ggaaaagcat ttgttattga
     1681 cggaggtcta tccaaagctt atcaaaaaac aaccgggatt gctggctact cattactatg
     1741 caattcttac ggtttccaaa tcgtgacaca ttatccgttc ttgcctattg aacaacaatt
     1801 tgcaaaaaaa tctgatcaaa cgaatgtaaa aaaagtgatc gagcagcagc tcccacgcaa
     1861 acttaatcgg aatacgacaa aaggaatgga attacagcaa cagattactc aactccaaag
     1921 gctgttggat tatcgaaagg acgattgaga aaaatgctag ccaacattgc tcaatagact
     1981 aaaaagcgac tgcaacaaaa ggcgtgtcgc agtctccttc ggaattagct caaaaaacgg
     2041 taaaatatta gaagctattt ttctgtttat ttctctaata ctcaggtcag ggcgctgatt
     2101 tcacgacctc ttccttaaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
     2161 ccaacaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttgaacggca
     2221 ggctcacgac ctaatcagaa aaacgattaa aattcattca caaagaaatc gaataaacgc
     2281 tgttcagatg aatctacacg atgagaaagt tccggatgcc attggacacc aaggattttg
     2341 tgcatattat ctttactttg gactgcttca atgagtccat cgttggaaaa ggcgatggct
     2401 tttaacaaag gagaaagatc tttgactgcc tgatgatgat aggaattcac aaagtatgaa
     2461 tcagggagaa ggtcacttaa taaactgtcc gaaacgattt tgacttcgtg agttgcaaac
     2521 tgcggggcag tgggctgttg ttcgtgtttt acggaccatt ttggatataa tgaaagatct
     2581 tgataaagtg ttccctccaa cacgacattc aatagttgaa gtcctcggca gactgcaaaa
     2641 atcggttttc tttgtttcaa tgcttccaag atcaatgcct gttcgaattg gtctcgttga
     2701 atatttgttt ccgtcagttt tggatgaggg tcttccatgt aaagttgcgg actaacatcc
     2761 tgaccgcctg caagaatgag tttgtcgatt ttctcaatta gttgaggcgc tgaatctgga
     2821 gacatgactg gcagaataag aggtaatcct tgagcatcta aaactgcatc cacaaatccc
     2881 tgcggtgtat agcttacttg atttccttgg aaagtatctg tcgctcgaat cagttgattc
     2941 ccggcaatgc cgatgattgg tttcattttt attcccccta aagtagacta attgtaacaa
     3001 ggatttaaaa aaatttaaac tatgtaagac ttgcccctat acttatctgt tcttgctgtg
     3061 ttactatctg taaagagaat tttcgtgtga aattcatgac gatagttgaa aatattgctt
     3121 aatttgttat aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct tcaaggatct
     3181 agttcttcaa ggggtgagaa ggacatttta ttgaaggggc ctgcctatac taagttatct
     3241 ttagaggtac atactttcaa tgaaaaaaga aagcatgtac ctctttttta aatcgaaagg
     3301 aagtgaagca agtgttagaa gctttggaac aaatccgaaa agcagaagaa gaaaatgaga
     3361 agcgtgctcg aaccctacgg cagcagttga agacctatgc agaagaaaaa gagcaagaat
     3421 tggagataat ccaaacgaac agtcggcaac agctgaaaca gttggtagaa gaaaaagaac
     3481 aattcgaaaa gcaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa gcagacaagg
     3541 tgcaagcaac tttgaaagac caatatcaaa agcatcagag tcaagcaatc ggtgcaatca
     3601 ttgaaagggt gaaagaaact tatggccgtc actaagatgg aaaaagtgac cctcatttca
     3661 gataaaagaa atcaggagag gattttacaa gcagtacaag gaatccaaaa tgttgaaatc
     3721 cgggatctgt atcaaaatag aacaaacaac cagtgggtag aaaaatattt tccagatacc
     3781 acgattattg ataaagaagc aaaattaagc gctttaggca atcggctgaa agacatccaa
     3841 gaagctatcc aattcataca acaccacggc gatagccgtc aaaagaaact ccatttgaaa
     3901 cgtcaagaac tttccttaca agacttggaa gcaaattatt ccgaagaaaa tttcatgaat
     3961 aaattagaag aagtccttga tttaaaagaa aagtgggaac agctaactga aaaacgtgat
     4021 cgtttagaag aagaggaaga atggcttctt cattggcaac agctggatat tgctccaagt
     4081 atgtatgcaa gtaagacgac catttttgag atggggactg tgagtgcagc aaattttgaa
     4141 agtttcaagg aagaactaag taagcttgat gacgtttata tggaagaaat agattatacg
     4201 ccaaaacaag tttatctttc gtatgttgct ttaaaaaaat cagttgctgc tatagaagaa
     4261 attgctggga aatacgggtt caataaagaa acgtatcctt atgaacaaaa tcctaaagaa
     4321 aaattgcaag aaaccaaaca gcaattatcc attgttcacg agcagcaaaa aaagataacg
     4381 acagcgttag gtcagtgcag cggctatatc caagaatttg aatgggtaga agaagtcaca
     4441 cttgccgttg cagaacggga aaaagtaaaa gaacggttta tccatgccac ttatctgatc
     4501 gttttgcaag gttgggtaga tgcagaagaa aaacagaatt tgatgcatgt gttaaacgaa
     4561 actgtatcag aagaagatat atacgttagt ttcgaaacac caaactcaga agagattcag
     4621 caggaagtcc ctactaaatt gaaaaaccat ccgcttatcg aaccttttga attgctgact
     4681 gaaatgtaca gtctgccaaa atatgaagaa gtagatccaa cgccttggat gacaccattt
     4741 tatcttgtct tcttcgggat gatggtcgca gatgttggtt acggattgct gatgctcatt
     4801 gggacaatac tagctcaaaa actatttgtt ttaccccgcg ggatgaaacg tttcgtaaaa
     4861 ttctttgaga ttctctcggt accatcgatc atctggggat tgatctacag ttcatttttc
     4921 ggacaggcat taccaaaaga actatttggt atccgcttgc ctttcccaat cctatctacg
     4981 acggatgacg tcaatacgat tttgatttta tccgtgattt tcggtctgat acagatcttg
     5041 gttggattat tcgtggcagc aaaggaaaat atcaggagga aagattactt gagtgctgtc
     5101 agtgatggtt ttgcctggca agggatcctg attgggatcg ttattgcatt gattggtgtc
     5161 atgttgataa aaaatacagc atttgtttat cttggcgcgg ggctatcgat cattgctgct
     5221 ttatgtatct taatcgtgcc gattatccaa tcgccgtcta aagtcaaggg tgctgccaaa
     5281 ggagcgtata atctttacgg attgactggt tatatcggtg atttagtcag ttatacacgt
     5341 ttgatggcac taggtatttc aggcggaagt attggcgcgg cattcaatat gctagttgct
     5401 ttcatgccgc cagccgctcg attcagtgta gggatccttt tgatcatcgc tttacacgca
     5461 ctgaatatgt ttttgacact gttaagtgcc tatgtccatg gtgcacgatt gcagtatgtc
     5521 gaattttttg gaaaatttta tacaggaggc ggacgtgcct ttgacccgct gaaaacggcg
     5581 gaaaaatacg tcaacatcaa tcataagaaa aaaaacaata aataattgga ggatttttat
     5641 acaatgatgg attacttaat tagtcaaaat ggtggaatgg tatttgcagt attagcgatg
     5701 gcaacagcga cgattttttc agggattggt tcagctaaag gtgttggaat gacaggtgag
     5761 gcagcagcag cgttaacaac aagtcaaccc gaaaaattcg gacaggcttt gattctgcaa
     5821 ttgcttccag gtacgcaagg gttgtatgga ttcgttatcg cctttttgat ttttatcaac
     5881 ttaggaaatg atatgtcagt tgtacaagga ttgaactttc taggcgcttc tctgccgatt
     5941 gcttttacag gtttgttttc aggtatcgcc caagggaaag ttgcagcagc aggtatccaa
     6001 attttagcga aaaaacctga acatgctaca aaaggaatca tttttgctgc gatggttgaa
     6061 acatatgcga ttttaggttt cgttatttcc ttcctattag tattgaatgc ctaagcataa
     6121 aggaggaaaa gcagatgaac gccattgaaa aaatcatctc tcagatgaat gaagcagcag
     6181 agcaagaaag agcggcgtta gagcaagaag agcgaatgaa gatcgatcaa aactttgaac
     6241 aaaaacgtac gcaaatcgaa acagaacatc aaaaacaaaa agaaaaacaa atcgaattac
     6301 ttgaaaaaaa ataccgtcag cttaggaatc gccaacaagt agaagtacga caagaaaatt
     6361 tgaatgcgaa gcaggaattt ttacgacgat tgtttgcaga tgcaatagct gaaatggaga
     6421 attgggatga atctgaacaa atccagttta tcaaaaattc attgtattca ttacctttga
     6481 caggcaaagt aacattcatt gctggagaaa aatcagcagc ttatctttct cagacattgc
     6541 tggatgaatg gaacaatgaa cttcccttta tgatggtttt aagtgatgaa actgttgctg
     6601 atcaagcggg ttttctgata aacgatcaag gagttcagta taattttctt ttcagcagcc
     6661 ttgtacaaga tatccaagga acgatgagtt ttgagattgc caatcaatta ttcgaataag
     6721 gagggatcag tgtatggaat accacgaact aaatcccttg attcgcggaa gagagcttga
     6781 acttttgtcc aatgagacat ttgaacggat gattcaaaca aattctgtcg aagcacttgg
     6841 tgaaatattg aaatccacga tttacagtcc atatatttat gacggatatg aatatgactt
     6901 cgaacagaat cttttaaagg agcagagccg tctatttcgc tggttaaagg aatcagcacc
     6961 ggagcctgaa atcgtttgga tctatacgat gcgctatact ttccataatc tgaaggtttt
     7021 gacaaaagcc gagatgacaa atcaaaatct ggaccatttg tatatcgacg atggatttta
     7081 ttcattagaa accttgaagg atgccattca tacgcaagtt tcaaatgaac tccctgaaag
     7141 cattatggac agtatacgag aagtacgtga ttattttgaa gagtctacca ttcttcaggg
     7201 aattgacgtg atttatgatc gacagttttt gacagagcag cgacggcttg ccgataagct
     7261 aggatatcaa gaattgctga cagaagtcat tgcttttatt gacttgacca atatcacaac
     7321 gatggctcga ggaatattgc agaaaagaac atatgggttc atgacgacgg tactttccag
     7381 ctcgggaagt atcgaaaaag aagtattttt gaactttgta gataaagata tggaagatta
     7441 cacgaatttt ctattagaga cagattatgc tgattttctg cgccctgtca tccgtgaggg
     7501 aaatcttgat ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt cattgtatca
     7561 agaagcacaa acccaggctt tcggaccact gccactacta gcctttttga acgcaaaaga
     7621 ggtagaaagc aaaaaccttc gtttactggt tatcggcaaa agaagccatt tcgaaccaga
     7681 aacaatcaga aagcgggtga gacaagtcta tgacgcataa gatcggtgta gttggcgaca
     7741 aagattctgt tttgccattc aaattgtttg gctttgatgt tcgttatggt acaaccaagc
     7801 aaaccgttcg tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg atttatgtca
     7861 cagagcaatg tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa caaatgatac
     7921 ctgctatcgt attgattccc aatcatcaag gatcattagg gatagggctg gaagaaattc
     7981 aaaaaaacgt agaaaaagca gtaggtcaaa atatcttata gtatgaaaag gagatagaaa
     8041 ttttgcaaat tggaaaaatc ataaaagtct ccggacctct tgtaatggct gagaacatgt
     8101 ctgatgccag tatccaagac atgtgtctag tgggagatct aggagttatc ggagaaatta
     8161 tcgagatgcg tggcgatgtg gcgtctattc aagtatatga agagacttct ggaattggtc
     8221 caggcgaacc ggttcgctct actggtgaag ctttatctgt tgaattagga ccagggatca
     8281 tttcacaaat gtttgacgga atccaacggc cattagatac ctttatggaa atcacacaaa
     8341 gtaatttctt agggcgcgga gttcaattgc ccgcattgga ccatgagaaa aaatggtggt
     8401 tcgaaccaac agtagaagcc ggagaaacgg tgagtgctgg agatgttatc gggatcgtag
     8461 aagaaacaaa agttataaaa cataaaatca tggtgccaaa tgggataaaa ggaacgatca
     8521 aaacaatcga aaaaggttca ttcactattg atgaaacagt atgtgtcatt gaaaccgaaa
     8581 atggtgacaa aaatttgacg atgcttcaaa aatggccagt aagacgtagt cggccaatca
     8641 aagaaaaatt aaacccggat gcacccatga tcactggaca gcgagtgatt gatacattct
     8701 tcccagtgac aaaaggagga gcagcagctg tacctggtcc ttttggtgca gggaaaacag
     8761 tcgttcagca tcaaattgca aaatgggccg atgtggacat ggttgtctat gtgggatgtg
     8821 gcgagcgtgg aaatgagatg acggatgttt taaatgaatt ccctgaattg attgacccta
     8881 atacaggaga atcgttgatg gaacgtacgg tattgattgc taatacatca aacatgccag
     8941 tagcagctcg agaagcttct atctatacgg gaatcacaat tgcggaatat ttccgtgaca
     9001 tgggttatga cgttgctatc atggcagact ccacttcacg atgggcagaa gcacttcgtg
     9061 agatgagcgg acgtttggaa gaaatgcctg gtgatgaagg ttatccagct tatcttggtt
     9121 cacggcttgc ggaatactat gaacgatctg gaagagtcat tgcattagga tcagaaggtc
     9181 gcgaaggaag tataaccgca atcagtgctg tatccccgtc tggaggggat atttcggaac
     9241 cagtcacaca aaatacattg cgggtcgtaa aagtattctg gggtcttgat tccagtttag
     9301 cccaaaaaag acattttcct tcgatcgatt ggatacaaag ctattctctt tattcaacag
     9361 aagtagggaa gtatatggat catatccttc aagctgactg ggcatccatg gtcaccgagg
     9421 gcatgcgtat tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg gtagggattg
     9481 attccctttc agataatgac cgtctgacat tagaagtggc aaaatccatt cgtgaagatt
     9541 atttgcagca aaatgctttt gatgatgtcg atacattcac ttcaagagag aaacaattca
     9601 aaatgttgaa gatgatcttg acctttggtg aagaaggacg ccgtgcgttg tcgttaggtg
     9661 cttatttaag tgagatcatg gacggtaccg tatcgattcg tgaaaggatc ggacgaagca
     9721 aatatatccc ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa atcaagcaaa
     9781 cgatgcaaac gatcgtttca gaaggaggca tgagcaatga ttaaagaata tcgtacgatc
     9841 ggtgaagtcg tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa atatgaagaa
     9901 ttaatcgaag tccgtatgca aaatggcgag attcgtcgtg gacaagtatt ggaagtccaa
     9961 gaagataaag caatggtcca aatattcgaa ggaacaagtg gtatcaattt acgtgactct
    10021 tctgtacgat ttttaggcca tcctttagaa ctaggtgtat cggaagacat gatcggtcga
    10081 gtattcgatg gtttgggacg accgaaagat aacggaccag agatcctgcc tgaaaagcat
    10141 ttggatatta acggagaagt aatcaatcca gtagctcggg attatccaga tgagtttatc
    10201 caaacaggta tctctgcaat cgatcatttg aatacattag ttcgtgggca aaaattacca
    10261 gtattttctg gctctggtct tcctcataaa gaattagctg ctcagatcgc gcgacaagca
    10321 acggtactcg attcatcaga tgatttcgcc gtagtctttg ctgctattgg aatcactttt
    10381 gaagaagctg aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga ccgttctgtc
    10441 atgtttatga atttagcaaa cgaccctgca atcgaacgta tcgctactcc tcgtatggct
    10501 ttgactgctg cagaatatct tgcttatgaa aagggaatgc atgttttggt tatcatgaca
    10561 gacatgacca actatgcaga ggcattgcga gagatttctg cagcacggcg agaagttcca
    10621 ggaagaagag gatatcctgg gtatctttac acgaacttag ctactttatt cgaacgagca
    10681 ggaagaatcc gcggactaaa aggatcggtt actcaaattc cgattctaac aatgccagaa
    10741 gacgataaaa ctcatccaat ccctgacttg actggttata ttacagaagg acagatcatc
    10801 ttgactagag agctttacaa gagtgggatt cagccaccaa ttgatgtact cccgtctctt
    10861 tcgcggttaa aagataaggg aactggaccc ggaaagacaa gagaagacca tgcagctacg
    10921 atgaatcagc tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt agccgtagtt
    10981 ttaggtgaat ctgctttatc tgatgtagac aagatctatg cgaagtttgc tgagcggttt
    11041 gaaaaagaat atgtcaatca aggattctac acgaaccgtt cgattactga aacacttgat
    11101 ctagggtggg aactgctatc gatgttgcca agaaccgaat taaaacgaat caaagacgac
    11161 atgctggaca aatatctgcc agaagggaag tgagttctga tggcacgatt gaatgtgaat
    11221 ccgacccgca tggaattgac tcgattgaaa aaacagttga caactgccac aagagggcat
    11281 aaattattaa aagataagca agatgaactg atgcgtcaat tcatcctgtt gattcgaaag
    11341 aacaaccagc tgcgtcaaga aatggaagag tctatgacag aagcaatgtc tgattttgta
    11401 ttggcaaatg cttcgatcac ggaacctttc atcgaagaat tatttgtttt gccggcggaa
    11461 aacgttgatt tgtctatcgt agacaaaaat atcatgagtg taaaagtacc tgtgatgaat
    11521 tttgaatatg atgaacagct gacagacaca cctttggaat acgggtattt gaattccagt
    11581 gccgaactgg atcggtcaat cgatcggttt actaccctgc ttccaaaact tttggaactg
    11641 acagaaatcg aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac acgtcggcga
    11701 gtcaatgcat tagaatatat gacgatccct caattagaag aaacgatcta ttatattcgt
    11761 atgaagctgg aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt aaaaaatatg
    11821 ggtaccactg actaagtggt atcttttttt aaaagaggaa aacgatgatt aaacaaagca
    11881 aacaaataaa gaaacgtttg ccggctgttc agcttctcgc attaggattt tttctcctga
    11941 ttttagttgg cggcggccta cttaccctac cattttttag taatagtggg catgggacaa
    12001 attttatcga tgcgcttttc actgcaactt ctgctgtctg tgtaacggga ttgacaacat
    12061 tgaatacagc agagcattgg aacagtgctg gtcaattttt gatcatgctt ttgattgaga
    12121 tcggcggtct tggttttatg atggttccga ttctatgctt tgcattgata aagaagaaag
    12181 tcagtctcag cacacgtatt atattaaaag aagcgctgaa tctagaagaa gtatcaggtg
    12241 tgatgaagct catgctttac atcctaaaat tggcctttgt tatccaattg attggtacgg
    12301 ttgctttatc ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc tggtacagtt
    12361 tgttccatgc aatctctagt ttttgtaatg cagggtttga tttgttagga gacagtttag
    12421 ccaatcacca gcaaaatgtt tatttgatca tggtggtatc tgctttgatc atttctggtg
    12481 ggttaggctt catcgtatgg cgtgatttac tggaatatca tcagaaaaag aagatgacga
    12541 ttcattcaaa aatcgcatta accgttacag gtaccctttt attaggcgga ttcgttgtgt
    12601 tctttttcac agagcgcaat ggggcaacgc tagtggaagg gaatatgttt caacgattgg
    12661 caaacacctt ttttatgagt gtgacaccta gaacggctgg atattattca gttgattacc
    12721 tccatatgag ccatgcaggt ctgattttaa cgatgttttt gatgtacatc ggcggaacat
    12781 ctgggtcgac ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg atccagatgc
    12841 gttcgatgtt gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc cgacaaggcg
    12901 cagtgttgcg ggcacttact ttattttttg ttacgttgac gttgtgtgta gtcgccatca
    12961 tgattttatc ggtgactgaa acgataccta aaacttctgg catcgaatat attgcttttg
    13021 aggtcttctc tgcgtttggt acagtaggtc ttacgatggg attgacgcca gacttgactg
    13081 caatagggaa attgatcatt atagcgctta tgtacattgg acgagttggt gtattgactg
    13141 tggtcttctc tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa tatccagaag
    13201 aaagtgtcat gattggataa tttcatcttc tgacagaaac ctagtgattt agtaaaataa
    13261 gaaaataaaa taatttttct tgcttcttgg atactttaag tgcaaaatgt tcgtttattt
    13321 tttgaggcac tacccgttgt tgaacctcgg gtagtgcctc ttttttattg aaaggaatca
    13381 ataagatttg attcctttat ttatcaatag gttataattc atattttttt attttttctt
    13441 ttacgatact tagtccagaa gctaaaattt caaggttatt agaggatagg gaaattcgaa
    13501 cgaatgcatc tggttgcgca cgtgtagtaa agcgtctgga atgataaagt cggataccgt
    13561 tagcaagaaa gtcattctca attatcttag ggtcaatctc aggatcaact ggaagaattc
    13621 gatagtaagg gacacgattc gttggttttt ctactttgaa aagagaatca aaaagctgat
    13681 tggctctttt tgtaagactt aatttagttt ctaagatcct atttgcttca tttgaatgga
    13741 ggatttgagt gatgacttcc acatctaatc cagaagtttt cacatttata ttaaaaattg
    13801 cttgttctat cttgctttgg acatgtactg gaactacaag ataagcaact cgcaatccag
    13861 aacaaataaa ttttgtcatt ccagctaagt agattgtttg gttaggtaat aaattttgga
    13921 aaggaggaat gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata tcatcttcaa
    13981 taacccatag accttctttt tcaatcactt cggctaattg ctctcgtctt ttgtgagaga
    14041 tttgaaaccc tataggatta ttacaagaag gcataagaaa gacaccatga atcttctttt
    14101 ttctacattc ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa tcaattggaa
    14161 cgatctctaa atgatataac tgagccaatt caatgaaatt agcgtatgta tatcggtctg
    14221 ttgctattct atcacctgga gagaaaagtg cagaaagtgt gatagccaat ccattttgaa
    14281 caccggaaac aatagataga gattctaagg acggaatgac tccttgagtt tttaaccaat
    14341 ttgctgcagt agtcaattga aaatttgtgc ctttgggatc ttggtaatta agcaaaccaa
    14401 caagatttgg gttattacta gttgattgaa taaacgggac aacgaattta ttacattctt
    14461 caaaagaact aaccagacca aaatcaatga tatgttcggt tacttcattg gtagagatgg
    14521 tgatggactc aaagacattg ggagaaataa atgtgccact tccaatattc gtatacagca
    14581 aacctttctc aattccatat ttataagcct tgccaacggt agtaaaattt aaatctaaat
    14641 aatcagctag ctctctttgg gagggtaact gagtgttttt ctgtaattta ttctttaaaa
    14701 tatcttgttc taattgttga atcaacgatc gataataagg acgcttcaat ttttgtttgt
    14761 ctggtcgcca agttaacggg taattgtcat aagaattaat aggcattttc tccactcctt
    14821 aaaattgtat accatacaat tttaccattg attgtattga ttttagaagt taatcttttg
    14881 tgaggaggga attgtctatg aaaattatta attttgagga agtagtagct tcttttagtt
    14941 ttgaagaagc gattcaagta atgaaaaaat gtttttttga ttatgaaagt ggtaaaatca
    15001 gccaaaatcc tcgattggtg gaaattttac ctgacggaaa aggcaaaaat atgtttgcga
    15061 tgatgcctgc ttatttgggg gagaacagat attttggcac aaaagtcatt accgcttttc
    15121 cggataatca tcagtatcat ttaccttctc atttaggaga gattttcttg tttgatgctc
    15181 aaaacggact acccaaagca ttgataaacg caaatgcagt cacctggatt cgaacagctg
    15241 ctgtatctgc gctggcaaca gattacttag caaatccgca tgcagaaaca cttgctttaa
    15301 ttggagctgg tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt cgtcccatca
    15361 ctacagtgtt tgtttacgat attttagaag aaagacgaaa tacttttatc gctaaagcta
    15421 aaaaacagta tcctaaaata acgtttataa atgcaatatc agtagaagat gcagtacgtc
    15481 cagcggaaat catttgtacg ttgacctcta gtaaagaggc atttcttcaa aaagagtggg
    15541 taagggaaga cgcacatatc aatgctgtgg gcacatttac tcctgataca agagagataa
    15601 gtacatcgtt gatggaaact tctcaaatct atgtagatga ttactcagct gccttaacag
    15661 aaagcgggga cctgcttata gccatggcag aaggagattt agaggaatct tgtattgttg
    15721 gtacattagg cgaattagtt tctgagaaaa aaacatttta taaaggagaa tcgaggccga
    15781 cgattttcga cgcagttggt ctagctattg aagatctttg ttgtgcagaa tatatttata
    15841 acaaattaga gaaaggggct ttaaaatgaa cttagattat tacgtagttg atgcatttgc
    15901 agatgaggtg ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat ggctgccaga
    15961 aggtacgatg caaaaaattg ccattgaaaa caatttatct gaaacagcat ttacagtgaa
    16021 gaaaaaccaa gaatttgagc ttagatggtt tacgccagat cgagaaatcg atctttgtgg
    16081 tcacgcgaca ttagcaacag catttgtttt atttaactat tacaaaatac ctgatgaaac
    16141 gattaaattt tctactcaaa gcggtaatct atttgtgaca agaattaaag attattatta
    16201 catggatttt cctagtatta tgccgaaaaa agtgccgata ttgtctgaat atgaagaagc
    16261 gattggggct aagattaaag aagcctactt agcacgtgat ttatttttcg tattagaaga
    16321 tgaaaaaacg gtggctaaat tgcagccaga ttttacagct atcaaaaatt ttgaattagg
    16381 aataggagtc atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta gaaccttctt
    16441 tccaaaatta acaatcaatg aagatccagt atgtggatca gcacattcta atcttattcc
    16501 ttattgggca gaaagaatga ataaaacaaa attgtcagct tatcaaatgt catcaagggg
    16561 cggggatcta gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa aagcagtttt
    16621 gttttctaaa ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa taaattggtg
    16681 gagagtggca accttatagc tacaatggtt gttaaaaaaa gacgcaaact ttttcaattt
    16741 aatgaaaagt ttgcgtctca ttttaattat tggtgtttgt tttttattca atcaagagta
    16801 aaatgcccca tactaactat cctcggatga cttcgatttt ataaccatca gggtcaatga
    16861 caaagtagta agaaggcgca gtgccaggta atccttttaa gtcagtaatc tcaagaccgg
    16921 cagctttatg ttgctcgtgt aatttttcta gatcatcagc tgctatagcg atatgaccat
    16981 agccgtctcc tagatcatac gcaccatgat catagttgta agtcaactct aattcatagt
    17041 catccccagg aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa tcacggcggc
    17101 ggctttcttc aaaaccaaat gccttttgat aaaattcgat tgatgcttca agatctttca
    17161 ctcgaacaca tgtgtgagcc attttcatag attcatctct ccttttcctt ttactcaatc
    17221 ataacaaaaa gaagtaagga aatagttgat ttgtgcttgt tcatgagaaa tttctcatgt
    17281 aaaatggata gaaaataaaa ctgttaaggt ggaaactatt catggataaa ccaatttttg
    17341 gtgaaaaaaa gcaatttgct gattatcaaa cccgttatgc agcatatatt gtcatagaaa
    17401 gagatcagca gattgcagta atcgaggctc caaatggtgc tttttttctt cctggcgggg
    17461 aaattgaagg agaagagacg aaagaagaag cgattgtccg tgagctgata gaagaaatgg
    17521 gaattgtggc agaaatttct tcttatctgg gagaagctga tgagtatttt tactcgaatt
    17581 atcgtgcaac ttattattat aatccaggat atttctttgt tgctgattct tggaagcgta
    17641 taggagaacc aactgaaaag accaataaaa tttggtgggt gagccctcaa gaagcgcttg
    17701 tgaaattgaa acgtggtagc caccgatggg cagtcgagag atggatcgaa aaaaataaaa
    17761 actgaaagaa agcgagtaga tcagctttct ctcagttttt ttatttatta cagtacatat
    17821 tttttgattg cttcagctac accgtgctcg tcgtttgatg aagtgatgac atttgctgct
    17881 tctttgacaa gagaaacggc atttcccatt gctactccta gaccagcata ttcaatcatg
    17941 gataagtcgt tctcgttatc tccgatagcc atgatttcat cttgatcaat accaaggtgc
    18001 tgtgcgagat tcgcaactgc cgcaccttta cttgcttctt tatttagtac ttcgaagtaa
    18061 tagggcgcac ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa acgcgcaatt
    18121 gctgcatcta aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt catgtctggt
    18181 gtcatttctt caggtgttcg atattttagc ggcatcttga ctaaacttgc ttcgtggatc
    18241 gtataagcgc tgatatctcg attaggcgtg tagatcgctt tgtcatcgat cgaatggaga
    18301 tgactgccga cacggcgtgc catcacttcg atctctagga aatcttcatg tgtcagtccg
    18361 tgacgggcga tgattttacc tgatttcgtt tgttgtacga gtgccccgtt ataagtaata
    18421 acgtaatcat tatcttgaaa aaggttcaac tcgtttaact gttcctggac acctggtaag
    18481 ggacggccag tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc aatcgcttct
    18541 tttacttctt ttgttacttc tcttttctca tttaataaag taccatcgat gtcgatagcg
    18601 actagtttga tagacataat ttcctccaat atattttaat ttatttcaat caatgcgcca
    18661 ttacggatat ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata atgatcacgc
    18721 aaggacggat caaccatttc ttttggaaag aaaaatcgct catctccttg ttcttgtccc
    18781 gctaatgcag caacaagctg actaacctgt gataattcta tcaaagttcc gtcatttcgc
    18841 aaaatctcga tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc ataaggtaga
    18901 tcgtagctag aattcacagc agtgtaataa acaggattat atccgacctt ttctactaac
    18961 aaagtgagtt cttggactaa ttttgaatcc cggttgccag aaaaagtagc agatttcagt
    19021 ggtttacgat tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc agattcagac
    19081 cattgagtaa agtaagtgcc caagacccca tcatcaagtt tgaggtattc ttctaaagta
    19141 aagcttccgt caagaaatgg cagaagtaat tgagaatgga gttcaaaact gctggcatct
    19201 tcttgataca actcgtgtgc gcgatgaagc agatgctcca aaattacttc cataccacgg
    19261 gaaacaggat ggaagtacac ttgcacatac atttgatatc ggctaacgat ataatcttcc
    19321 actgcatgca tgccactcat ggaaaaagcg atcccgcctt cataaggacg aatcacacgt
    19381 aatattcggg taagatcaaa tgttccatat tctgttccag tgaaataagc atcccgaagc
    19441 aaatagtcca ttcgatctgc atcgatctga ctagaaatca tttggacaac ttgtgggttg
    19501 gggtaagttt tttggatcac actagctacc ttttcaggaa aaccttcttc tactcggtta
    19561 agaatctgat acacttctgt ttctggagaa gtgatgatct ctacagtgat ggcttcatga
    19621 ttcgtatgga agatatgttc aaatgtatga gagtaaggac catgaccgac atcatgcaac
    19681 agtgccgcac ataaagtcac cagccgttcc tgatcatccc agcctccgtt gcctattttt
    19741 tcttttgaaa aatttcgaga aaagatatcg cagattcgtc ttgaaatctc ataaacgcct
    19801 aatgaatgag taaaacggct atgttctgct ccgtgaaaag tgaaagaaga agtgcctaat
    19861 tgtttaatgc gtcgtagacg ttggacttct ttcgaattga tcaaatctaa gattacttga
    19921 tgctggacat ggatatagtt atggactgga tcacgaaaaa ctttttccat aggcagcatt
    19981 tgatatttat aaggggttgt cataatgtag ctcctttatg ttattacgtt gttccattct
    20041 ctggatttgg ttgttccatt gctccagctg gatttctttt tttagccagt cggtagaatc
    20101 ctgtaaatca cttgcagaat acagcttttc aaaagctttt gcaaatgctt ctttgacttc
    20161 ttttacaaaa aggggttggt caagcagttc ttcaagtgtt gccattgtag caggatccac
    20221 tgcaggatag ccatttgtac caaagtcttc ttttaaagca gattgataaa atcgcttcat
    20281 gacttgcccg cgtttttcct gattaccgtt gacacttaag tacatcataa cagaaatccc
    20341 atttttgatc cgacgctgag cgataccggc aaattttttg ccatggatgc ttaaatcata
    20401 agttccagga caataagaat gggtgatttc gaatgcttgt atcggtgcct ttggaaaagc
    20461 gtatttagtc aataggagca tttgctcgta agcgtcatct atggttattt tttttgtgtc
    20521 actttggggg aaaatcaaag aaatattcaa aattccttca tctgatacga tccctaatcc
    20581 acctgaattt ctgatcactg gagcgtaacc tgcatctttc agtgtttgga tcccgtcact
    20641 tagaaaagga gttcgaacat ccttcatacc taagatgact gtgcagtcat attgccagaa
    20701 atgtaaaaag atttgctggt gttctttaga atattccgtt aatatatctg ttaatgcaaa
    20761 aggggaaaag tcgttttttt gataaatgtg ctgatctagt cgaatcgcag ggttcattca
    20821 accggctcct ttgtataaat tcatattttc tacaatatta tactctaaaa tcaaagagtt
    20881 gttttggaaa aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac aaaagaaact
    20941 aggaacaatg tagctatgag aagtaaagga gaatgaataa tggaattatc acaaggaaca
    21001 ccgatttcaa tcaaattgcg tacaaaagta aaacagcaag gggaagtgca agatttttat
    21061 tttgatttga aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg ttataaagag
    21121 attcaggagg agacgggcga tgagattcct gtaacgatca aattagttcc agatggtcat
    21181 gtacagctga tccgtgcagg agaaatgcgg atgcgtttga aatttggcta taaagaacgg
    21241 ttagaaacat cctatcgaac accatacggt atgatccaga ttgctacttt tacgaaagaa
    21301 ctgcatgtta gtttgaaaga ccgtcccaca ggagggaagg ttcgaatcga ttatgatctg
    21361 ttcatgggac cggaaaaaat cggcgaatat tatttgacct tggattttac agcgtaaata
    21421 taaaagaaaa gtttgatttt ttgtgtctga ttttgtatga taaggagtag actgtgaaag
    21481 gacgtgtcct gattggaaat tagtgtattt gaaggtgcaa acaaaagcga attatcaatg
    21541 atcgaagtag cacacgcgat cttagaacaa cgtggcgatg tcatggattt ttctgattta
    21601 gctaaccaga tccaaaacta tcttgggaaa tcagacagtg aaattcgtga ttcactcgca
    21661 caattttata ctgacttgaa tattgacggc agctttatct cattagggga taatcgttgg
    21721 ggcttgcgtt catggtatgc tatcgattcg atcgatgaag aagtaaacca tggaatggac
    21781 gaagaggatg aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc ctttatcaat
    21841 gatgatgaag acgcgatcga ttacaatgac gatgacccag aagatacaga tctgacagaa
    21901 gaagatgacg atgatctgtt tgatgacgat gatgatgaag atgaagaaat cgctgcttat
    21961 aattctgact tgcaagaaat cggagcagat gacccgacag atgatgaaga agacttgcca
    22021 ggaaatatcg aagaagattt aagtatcata gacgaagacg atgatgatga agattatgaa
    22081 gaggaagaat tttctgactc tgaaaaagaa taaacattat tttcttacga gaggttgtga
    22141 cagagacgat cagcctatca gaataaggag gtgtgacacg at
//
LOCUS       DAZUKZ010000035        22031 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000035 DAZUKZ010000000
VERSION     DAZUKZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.035"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            367..555
                     /locus_tag="ACG8EF_001305"
     CDS             367..555
                     /locus_tag="ACG8EF_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946619.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="ACG8EF_001306"
     CDS             545..805
                     /locus_tag="ACG8EF_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6275 family protein"
                     /protein_id="HFS4946620.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="ACG8EF_001307"
     CDS             846..1073
                     /locus_tag="ACG8EF_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946621.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="ACG8EF_001308"
     CDS             1138..1710
                     /locus_tag="ACG8EF_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946622.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="ACG8EF_001309"
     CDS             1711..2022
                     /locus_tag="ACG8EF_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4946623.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="ACG8EF_001310"
     CDS             2013..2273
                     /locus_tag="ACG8EF_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946624.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="ACG8EF_001311"
     CDS             2434..2793
                     /locus_tag="ACG8EF_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946625.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="ACG8EF_001312"
     CDS             2790..4433
                     /locus_tag="ACG8EF_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS4946626.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="ACG8EF_001313"
     CDS             4468..5550
                     /locus_tag="ACG8EF_001313"
                     /inference="COORDINATES: protein motif:HMM:NF028182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage abortive infection protein"
                     /protein_id="HFS4946627.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="ACG8EF_001314"
     CDS             5594..6766
                     /locus_tag="ACG8EF_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4946628.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="ACG8EF_001315"
     CDS             6753..7457
                     /locus_tag="ACG8EF_001315"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS4946629.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="ACG8EF_001316"
     CDS             7462..8622
                     /locus_tag="ACG8EF_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4946630.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="ACG8EF_001317"
     CDS             8698..8976
                     /locus_tag="ACG8EF_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFS4946631.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="ACG8EF_001318"
     CDS             8957..9328
                     /locus_tag="ACG8EF_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFS4946632.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="ACG8EF_001319"
     CDS             9344..9748
                     /locus_tag="ACG8EF_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946633.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="ACG8EF_001320"
     CDS             9745..10155
                     /locus_tag="ACG8EF_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946634.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="ACG8EF_001321"
     CDS             10216..10803
                     /locus_tag="ACG8EF_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFS4946635.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="ACG8EF_001322"
     CDS             10876..11343
                     /locus_tag="ACG8EF_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HFS4946636.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="ACG8EF_001323"
     CDS             11389..11697
                     /locus_tag="ACG8EF_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946637.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="ACG8EF_001324"
     CDS             11892..18626
                     /locus_tag="ACG8EF_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4946638.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKGFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18657..19502
                     /locus_tag="ACG8EF_001325"
     CDS             18657..19502
                     /locus_tag="ACG8EF_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFS4946639.1"
                     /translation="MNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSFREIGESIEL
                     SEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSLKSLSKTEID
                     SQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYTKNVWEKKGV
                     FKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFINMKDTQILE
                     VSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGHANVEIELYE
                     ERDLF"
     gene            19499..20548
                     /locus_tag="ACG8EF_001326"
     CDS             19499..20548
                     /locus_tag="ACG8EF_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946640.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="ACG8EF_001327"
     CDS             20552..21376
                     /locus_tag="ACG8EF_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946641.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..>22031
                     /locus_tag="ACG8EF_001328"
     CDS             21394..>22031
                     /locus_tag="ACG8EF_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFS4946642.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7877 a         3297 c         4672 g         6185 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa aggattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DAZUKZ010000036        21988 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000036 DAZUKZ010000000
VERSION     DAZUKZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.036"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACG8EF_001329"
     CDS             <1..395
                     /locus_tag="ACG8EF_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS4946643.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="ACG8EF_001330"
     CDS             398..1147
                     /locus_tag="ACG8EF_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4946644.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="ACG8EF_001331"
     CDS             1159..1428
                     /locus_tag="ACG8EF_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946645.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="ACG8EF_001332"
     CDS             1433..1597
                     /locus_tag="ACG8EF_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946646.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="ACG8EF_001333"
     CDS             1590..1892
                     /locus_tag="ACG8EF_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946647.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="ACG8EF_001334"
     CDS             1889..2050
                     /locus_tag="ACG8EF_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS4946648.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="ACG8EF_001335"
     CDS             2047..2352
                     /locus_tag="ACG8EF_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946649.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="ACG8EF_001336"
     CDS             2352..2708
                     /locus_tag="ACG8EF_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFS4946650.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2668..2913
                     /locus_tag="ACG8EF_001337"
     CDS             2668..2913
                     /locus_tag="ACG8EF_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946651.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="ACG8EF_001338"
     CDS             2910..3329
                     /locus_tag="ACG8EF_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFS4946652.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="ACG8EF_001339"
     CDS             3326..3883
                     /locus_tag="ACG8EF_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFS4946653.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="ACG8EF_001340"
     CDS             3880..4176
                     /locus_tag="ACG8EF_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946654.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="ACG8EF_001341"
     CDS             4253..4666
                     /locus_tag="ACG8EF_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS4946655.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="ACG8EF_001342"
     CDS             5012..5386
                     /locus_tag="ACG8EF_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946656.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="ACG8EF_001343"
     CDS             5423..5590
                     /locus_tag="ACG8EF_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946657.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="ACG8EF_001344"
     CDS             5616..5960
                     /locus_tag="ACG8EF_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS4946658.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="ACG8EF_001345"
     CDS             5965..6246
                     /locus_tag="ACG8EF_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946659.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="ACG8EF_001346"
     CDS             6349..6663
                     /locus_tag="ACG8EF_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFS4946660.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="ACG8EF_001347"
     CDS             6641..8335
                     /locus_tag="ACG8EF_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS4946661.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="ACG8EF_001348"
     CDS             8355..9533
                     /locus_tag="ACG8EF_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4946662.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="ACG8EF_001349"
     CDS             9496..10182
                     /locus_tag="ACG8EF_001349"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS4946663.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="ACG8EF_001350"
     CDS             10182..11342
                     /locus_tag="ACG8EF_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4946664.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="ACG8EF_001351"
     CDS             11352..12227
                     /locus_tag="ACG8EF_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946665.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="ACG8EF_001352"
     CDS             12224..12535
                     /locus_tag="ACG8EF_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946666.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="ACG8EF_001353"
     CDS             12525..12878
                     /locus_tag="ACG8EF_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS4946667.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="ACG8EF_001354"
     CDS             12868..13269
                     /locus_tag="ACG8EF_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946668.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="ACG8EF_001355"
     CDS             13262..13666
                     /locus_tag="ACG8EF_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946669.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="ACG8EF_001356"
     CDS             13678..14286
                     /locus_tag="ACG8EF_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4946670.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="ACG8EF_001357"
     CDS             14306..14668
                     /locus_tag="ACG8EF_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946671.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="ACG8EF_001358"
     CDS             14671..14853
                     /locus_tag="ACG8EF_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946672.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="ACG8EF_001359"
     CDS             14870..18289
                     /locus_tag="ACG8EF_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4946673.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="ACG8EF_001360"
     CDS             18340..19077
                     /locus_tag="ACG8EF_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4946674.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="ACG8EF_001361"
     CDS             19087..21378
                     /locus_tag="ACG8EF_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS4946675.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="ACG8EF_001362"
     CDS             21402..>21988
                     /locus_tag="ACG8EF_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFS4946676.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3245 c         4705 g         6105 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DAZUKZ010000037        21911 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000037 DAZUKZ010000000
VERSION     DAZUKZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.037"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..1245
                     /locus_tag="ACG8EF_001363"
     CDS             76..1245
                     /locus_tag="ACG8EF_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4946677.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1819..2388
                     /locus_tag="ACG8EF_001364"
     CDS             1819..2388
                     /locus_tag="ACG8EF_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFS4946678.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            2375..3235
                     /locus_tag="ACG8EF_001365"
     CDS             2375..3235
                     /locus_tag="ACG8EF_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4946679.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            3425..4129
                     /gene="yycF"
                     /locus_tag="ACG8EF_001366"
     CDS             3425..4129
                     /gene="yycF"
                     /locus_tag="ACG8EF_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFS4946680.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            4134..5969
                     /gene="walK"
                     /locus_tag="ACG8EF_001367"
     CDS             4134..5969
                     /gene="walK"
                     /locus_tag="ACG8EF_001367"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFS4946681.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5966..7282
                     /gene="yycH"
                     /locus_tag="ACG8EF_001368"
     CDS             5966..7282
                     /gene="yycH"
                     /locus_tag="ACG8EF_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFS4946682.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            7283..8152
                     /locus_tag="ACG8EF_001369"
     CDS             7283..8152
                     /locus_tag="ACG8EF_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFS4946683.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            8207..9016
                     /locus_tag="ACG8EF_001370"
     CDS             8207..9016
                     /locus_tag="ACG8EF_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4946684.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(9119..9748)
                     /locus_tag="ACG8EF_001371"
     CDS             complement(9119..9748)
                     /locus_tag="ACG8EF_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4946685.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9918..11210
                     /locus_tag="ACG8EF_001372"
     CDS             9918..11210
                     /locus_tag="ACG8EF_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4946686.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(11286..11948)
                     /locus_tag="ACG8EF_001373"
     CDS             complement(11286..11948)
                     /locus_tag="ACG8EF_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS4946687.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            12173..12457
                     /gene="groES"
                     /locus_tag="ACG8EF_001374"
     CDS             12173..12457
                     /gene="groES"
                     /locus_tag="ACG8EF_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFS4946688.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12508..14133
                     /gene="groL"
                     /locus_tag="ACG8EF_001375"
     CDS             12508..14133
                     /gene="groL"
                     /locus_tag="ACG8EF_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFS4946689.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(14222..15361)
                     /locus_tag="ACG8EF_001376"
     CDS             complement(14222..15361)
                     /locus_tag="ACG8EF_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4946690.1"
                     /translation="MWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWKKAQ
                     KILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHISDD
                     TIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPALAVK
                     IHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRYGEL
                     LALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIESVIA
                     DNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSHVSL
                     LSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(15472..16056)
                     /locus_tag="ACG8EF_001377"
     CDS             complement(15472..16056)
                     /locus_tag="ACG8EF_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFS4946691.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(16183..16614)
                     /locus_tag="ACG8EF_001378"
     CDS             complement(16183..16614)
                     /locus_tag="ACG8EF_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS4946692.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(16632..16952)
                     /locus_tag="ACG8EF_001379"
     CDS             complement(16632..16952)
                     /locus_tag="ACG8EF_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4946693.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            17252..17392
                     /locus_tag="ACG8EF_001380"
     CDS             17252..17392
                     /locus_tag="ACG8EF_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946694.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            17690..17947
                     /locus_tag="ACG8EF_001381"
     CDS             17690..17947
                     /locus_tag="ACG8EF_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HFS4946695.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(17918..18226)
                     /locus_tag="ACG8EF_001382"
     CDS             complement(17918..18226)
                     /locus_tag="ACG8EF_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946696.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            18304..19050
                     /locus_tag="ACG8EF_001383"
     CDS             18304..19050
                     /locus_tag="ACG8EF_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS4946697.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            19065..19268
                     /locus_tag="ACG8EF_001384"
     CDS             19065..19268
                     /locus_tag="ACG8EF_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4946698.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            19373..19672
                     /locus_tag="ACG8EF_001385"
     CDS             19373..19672
                     /locus_tag="ACG8EF_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946699.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            19672..19854
                     /locus_tag="ACG8EF_001386"
     CDS             19672..19854
                     /locus_tag="ACG8EF_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946700.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(19860..20336)
                     /locus_tag="ACG8EF_001387"
     CDS             complement(19860..20336)
                     /locus_tag="ACG8EF_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946701.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            20389..21114
                     /locus_tag="ACG8EF_001388"
     CDS             20389..21114
                     /locus_tag="ACG8EF_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFS4946702.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            21288..21629
                     /locus_tag="ACG8EF_001389"
     CDS             21288..21629
                     /locus_tag="ACG8EF_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946703.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            21622..>21911
                     /locus_tag="ACG8EF_001390"
     CDS             21622..>21911
                     /locus_tag="ACG8EF_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS4946704.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         7461 a         3638 c         4383 g         6429 t
ORIGIN      
        1 atgaaggtaa aaatcatgct ataataaaat agtcaaaatt tttgccgata tttcagttta
       61 agaggagaga ttgaattgaa attaagagca aacataattg aaaaaagagg aacattacaa
      121 gtagttgtat cttataaaga ctctaacgga aaaattagac aaaagtggaa agatacaaaa
      181 ttaaaaaaga aaggaaataa aaaacgagca gaaaaaatta gagatgaatt tttactagaa
      241 ttggaaagtc aattagctat ggaatgtgaa tgtgctaatc caaatttatt gttttatgac
      301 tatttgctta attatttgga agtatcaaaa aaacaagtag catttaacac atatgttggg
      361 tataagcatt acgtccataa tcgaatttac aagttttttt atcctaaaaa aattaaacta
      421 aataggttaa agccttatca tttacaagag ttttatcaat atatgttaaa tgataactgt
      481 acagcaaata cggttattca ttatcacgca tttatacgca aagctcttca agaggctgtt
      541 attacagaat tgataagtac aaatgtagca gataaagtaa gaaagccaaa aaagaaacaa
      601 tttgtttctc aagtttataa tcaacaagag atcttgaaat tacttgacat tttgtcagaa
      661 gaaaaactct atttggtagt tcttttaaca gcattttatg gattgcgaag aagtgaagtt
      721 ttaggattaa aatggtctgc tattgatttt ttaaacaaaa aaatcatgat aaaccatgta
      781 atcatagaaa atccagagaa tatgtcacag ttaataaaga aagatcaaac aaagaactct
      841 tccagctatc gtacattacc tttggttgaa acgattgaac gagcattagt aaatcaagct
      901 aaatggcaac aagataatcg tattctttta ggagatgatt atattttaga agataatgag
      961 tacgtattta ctatggaaga tggacggttg atgaagcctc aatatgtaac gcatcgtttg
     1021 agtaagttga ttaagaaaaa tggattgaaa aaaattcgct ttcatgactt gcgtcattct
     1081 aatgcttcaa taatgttaga tagtggccag aatatgaaat ccattcaaga atggctggga
     1141 catgcatcgt attcgacaac tgctaattta tacacgcatt taaccgcagg agtaaaagat
     1201 cgtgcagcag aagcattaga aaacgcattt gggtttaaag aataaaaaaa caaaaaacaa
     1261 tatctttggg gtatcgcttc ttgtttttag aatttttttg ttggcaattt gttggcagta
     1321 tgttggcaat gaaaaaattc acaaactaaa aacctattta taaagcttta taaaactttt
     1381 ttcacaaata gagcatttgg ttaagagtca gctgctctac caactgagct aacggcccgt
     1441 acaaaaagta cttaataata gtagcactaa gaattttaaa atgcaagact acatttaaag
     1501 aaaatcagaa aaatctttac ttccttatag gaaatcattc atagattatt cacatttgct
     1561 cgctattata taagcatacc aacaaacaaa gttcttgcaa gagaacaaac cctaacactt
     1621 ttttcatttt ttactccttt cccgccgtct ccccggcggg tctcctgttc ttaacgataa
     1681 aagacaagaa ggcttagcct tcttgttttt tgttttcatg gaatcccgct aatcaaatca
     1741 cctagttaaa gcaactagat tgaatgttta tcataagtct gatatcatgt ttatgaaatg
     1801 aaaaaaggag cggttatgat ggaaaaaatg cgtcagcaat taatggagtg ggggaaggaa
     1861 ctagaaacat tccgtctgcc tcactgggaa gaactccctg acttagaact ttatatggac
     1921 caagtcagaa cgttagttga tcgttatctt tcacctgtga tacaaggaga aaagcatcca
     1981 ttgcttacct cttctatggt aaacaattac gttaaactag gtttgatacc agcaccagtt
     2041 aaaaaaagat ataacaaaga acacgtagca tttttacttg caatcactac gttgaaacaa
     2101 gtattgacta ttcctgaaat caaagaaggg atattgttcc aaggaaaaac agtaggaatt
     2161 cgtgaagcct ataatctatt ttgcgatgaa caagaagcag ctgtgtggat ggtcagccaa
     2221 ttagcacaag gtaaatctca tccgcaaaaa ttcgaccagg caacccctgt agaatatatt
     2281 gctgtaagag cggcaacttt atcgtttgca atgaaacttc ttgcggaaaa aacgattgtt
     2341 ctagaaaccg aatatttaaa agaggagaaa acaaatgaaa aacaataaaa tcgcaatttt
     2401 agtagattca ggtacggatg ttccagaaga attgataaaa aaatacgaga tgtatatgat
     2461 cccattgaaa attatttata aagatcaggt atatacagat aaaattgaca ttacccctga
     2521 agagatctat caacgtttgc cagaggaaat ccctggtaca tctttgccgg atggtgagtc
     2581 gatcacagaa atctttgagc aaatcaaaaa agacggttat gaaaaggtac ttgcggtaac
     2641 aatttccagt ggattgagcg ggacatataa tgtggttcgt ttaattgcac aacagcaaga
     2701 agaattggaa gcatatgtac tagatacgaa aaatattggt attggtgcag gtttttctgc
     2761 gatccaagca gcaaaatggc tggaagaagg agtagaatgg gatcaactga tcagtaatct
     2821 gaatgagtta gtcgaaagaa caaaagtttt ctttaatgtt gcgacattgg aatatcttca
     2881 aaaaggtgga cggatcggtt tagttgcttc tattgtagga accgctttaa agcttaaccc
     2941 aatcatctca tgtaatgaag aagggattta ttacacagta ggaaaagcta ggggacgtaa
     3001 aaaaagtttg gataaaacgg ttgcttacgt caaagaaaaa atcggtaatg caaaggtctt
     3061 caacttagct gttgcccatg gggatgcgaa agaagaagca attgaaatgg aagcgagact
     3121 taaaagagag ttcccacaag caaaccaaat ctactttggc cagatttctc cggcattagt
     3181 agtccacact ggtccgggat tattgggtgt cggtgtacaa gttttagaag gataaatcaa
     3241 aaaaaggaaa cactgaggtg aaaatcagtg ttttcctttt ttattgaagt tagaacaaaa
     3301 aataataaag tattttgcac tttcatttta attttctctt ttgttttata ggcaaagatg
     3361 aaacaaaagc agaaatacgg tataatgaga aggtatgaca ataatatata agaggtggct
     3421 ctttatgaaa aaagttttag ttgtcgatga tgagaagccg atttcggata tcgtcaaatt
     3481 caatttagcc aaagaaggct atgacgttta tactgcatat gatggggaag aagccttgga
     3541 aaaagttgct gaggtagaac ctgatttgat tttacttgat ttgatgctac ctaaaatgga
     3601 cggcttagaa gttgctcgtg aggtacgcaa aacttatgat atgccgatca ttatggtaac
     3661 agccaaagat tctgaaattg ataaagtcct aggcttagag ttaggcgccg atgattacgt
     3721 gaccaagcca ttttccaacc gagaattagt tgcacgtgtc aaagcaaatc ttcgtcgcgg
     3781 tgccacggct gcgaaggaac cagaagaggc agcacctgcg gaattgacga ttggtgattt
     3841 gacgatccat ccagaagctt acatggtgac gaaacgagga gaaacaatcg agttaacaca
     3901 tcgtgaattc gaactactct tttacttggc gaagcacctt ggacaagtga tgacaagaga
     3961 acatttatta caaacggttt ggggttatga ttattttgga gatgtacgaa cagtggatgt
     4021 aactgtacgt cgtttgcgtg aaaagatcga agataatcca agccatccaa attatttagt
     4081 gacacgtcgt ggtgtcggtt actatttgcg taacccagaa caggagtaat tggatgaaaa
     4141 gaaaagtccg cttttttcga tcagtcaatt ttaagatcgc tattaccttt attctgattt
     4201 tattgatctc gatcgaaatt attggtgcct actttatacg tggtttagaa cgttccacga
     4261 tcaatacatt tattaaagat atgaatcaaa cggtggaatc attagcaaca acaatcagcc
     4321 cggaattgaa tcgaaaagat aatgcagatg atgaagaggt aaatgcgaat atcaaaagat
     4381 ttattgaaaa cagtgcgact agcgatatca tcgaaatacg ggtcgttgac gaaaaaggga
     4441 tcatccgagg aacaaccgat gtcaatgaac aaagcgctgt gggaaagaaa aatgattatg
     4501 ttgatatcaa tgatttcacg actaaacgtt atgttgcttt agataacgac gataaacggg
     4561 tgaatatcaa tgttcaaccg atcctgtccc cgaccggcga tgccgttatc ggggctcttt
     4621 acgtcaaaag taatatcgaa caaaaatatt cggaaatcaa taacacagca gtcattttct
     4681 ttactgcctc actaattgcg gcatttattt caatgatcgt atctgtttta gttgctcgtt
     4741 cgattacaca gccgatcggt gaaatgagag aacaagccat tcgaatcgct agaggagact
     4801 acagtcggaa agtcaaagtg catggacaag atgaacttgg acagctggct gataccttca
     4861 atcagttagc tgaacgtatt gaagaagctc aagatacaat ggaagctgaa cgcaatcgtt
     4921 tggatagcgt gctatcccat atgacagacg gcgtcattgc gacagatcgc aggggtaaag
     4981 tcattacgat caatgatatg gctgtttcct tgctagatat caaaaacgag gaagcaatcg
     5041 gacaatcgat tttgacactt ttagatatcg aagaagaata tacgttgcgg aaattattag
     5101 aagatccaaa cgaaatggta cttgaccgtt caaccagtac attggaaagt gatcagatga
     5161 tcttgcgagt agacttttct atgatccgtc gcgaatcagg ttttatcagc ggattagtag
     5221 ctgtcttgca tgatgtgaca gaacaagaaa aaacagaacg ggaacgtcgt gaattcgtct
     5281 ctaatgtttc ccatgaactt cgtacaccgt tgacaagtat gcgtagttat atcgaagcat
     5341 tgagcgaagg tgcgtggaaa gacgaggaag tagcaccaaa ttttttgaag gtaacgctag
     5401 atgaaacaga ccgaatgatt cgaatgatca atgacctctt aaatctatct cgcatggata
     5461 caggtaatac ccagcttcaa ttagaatatg tcaatttcaa cgagttagtg aattttgttc
     5521 ttgaccggtt tgatatgatg gtgggaaatc aggaaaaaaa ttataagatc cgtcgtgaat
     5581 ttacgcaaag agatctttgg gtcgaagtag atacagataa aatcatccaa gtagtcgata
     5641 atatcatgaa taacgcgatc aagtattcac cagatggggg aacgattact tgtcgcttat
     5701 tagaaacgca taataacgtg attttaagta tcaccgatca aggattgggg attcctaaaa
     5761 aagatctgaa ccgagtgttc gagcggttct atcgagtaga taaagcacgt gcaagagcac
     5821 aaggtgggac aggacttgga ttagctattt ctagagaagt gattaaggcg catcgaggcg
     5881 ctatttgggc agagagcaag gaaggaaaag ggtcgacttt ttatatctcg ctgccatatg
     5941 aaccttatga ggaggaatgg tgggaatgag aatctctgaa aaagtcgttc gtataggact
     6001 cattttgatg gttgcgttaa gcatatactt ctcttatgca atctggctga gcccagcagg
     6061 aaaaagttca atcaacgtag atgagacaaa cactcaaatg atcgagtctc aaagcaatcg
     6121 aaaaacttcc gaagtctttc ttccgcttca tgttacatgg caccattcgg gaatgatcca
     6181 agaaaccaac agtgaaaatc tcgtgtaccg attgcaattg atcatcgata aagcaagatt
     6241 tggaaagctt tctgaagtag tttcaggaga caaatcacag tttgaacagg tgaaaaatct
     6301 gaactctggt tttgagttgg cctataatgc gccttttttg ctaagagaat acaaggaagc
     6361 atttgattta gatttagatg tgtcgaactt ggcaaaagaa agcgaaaata tgtattttac
     6421 tagaatacaa ttcgatcaag gaaataaaaa aatccgattt ttgaattatt cgaaccatct
     6481 cgtatatgaa gcaaatattt cgttgaattg gacagatatc aataaagaat taacagcaaa
     6541 agacacaaaa tggacagaaa tgacaacaga accatcgacg atcgacacgc aatatgctac
     6601 gaaacattcg atcaaactga agaagtatag ttatatcctg tcacagcaac cctatacact
     6661 ttttagaaat gcttttttcc agaaaccaga tagcgtgaaa aataatgatg aaagcaatga
     6721 attgattttt tacggtggga atgaaacaat gtctatccat tcggaaacgc aagtagttga
     6781 ttttcgtgga gagctgccag agaaaaaaga aaatgctagt gtattttctg aaagtttccc
     6841 ctatattagc aaattaggca gttctttagg gaacctcaga tattttgacc gcagtgacaa
     6901 tacgatcgat tatcggatat tcgtggaagg cttcccggtt tttgggacag acagcagagg
     6961 acagattggt gtggaagttg gcggtgaaac gaatggttct gtgcaagtga atatccaaac
     7021 cagtttgaat acgattcaag tgccgatacc ttcagacgaa gaagtagaac ttgcaagtac
     7081 gaatgaaatc atccagcagt taaaagctaa gggagcagat agcaacaaga ttggttcagt
     7141 gatcgttggc tatacatggc aaaatgtcaa agaaacgaat cgtgtcgttg atctcttacc
     7201 tgaatggtat gtgaaatata aagataactg gtattcagct aacgaccttc tggaaaaatt
     7261 acctggattg gaggcgaatt aattggattt caaaaaaatc gaatggatat ttttcgtcgc
     7321 ttttttaggg ttgaatgtgt ttttattcag cagttatcat gacgcggtat accaagaaaa
     7381 caatgtcatt cgctctaatc agactgaatc aatccaacaa cgtctggcta gcgacgatat
     7441 cacttacacg gggaagattt cgtctgagaa caagcaaggc tattatctta gcgcggagca
     7501 gaccaatctt agcacttcat tagcgaatta tcgcaaggct ggtaaaagcg gcttgctggc
     7561 aagtggtgtg tttttagacg gtgatgtttt gacgaaatct ttatccagtt cagatgctga
     7621 aaagaacgtg atcgatccag ataaaattag tagttccttg gaagattttt tatctaacaa
     7681 agacaatatt ttattcggta atgattatgt gtacacgaaa aacttttctc atttagaaga
     7741 agaaccacta gaaatccaag caagccaaga gtatgaaggc atcccattca atgatgatac
     7801 agcaaaaatc gttctttctt tagaaaaaaa cgataatgat tatagcatca cgaaatacat
     7861 gcagacgcat ttatctgata tcgagcagct gcgcgaaaag acggaactgt atacggaaga
     7921 ggaagcaatc aatacattgt acatcaataa caaaatacca agaggatcaa aaattttatg
     7981 gcgtcagcta gcttattcct gtattctgaa agtccgggaa aaaaatgtgt atgttcctgt
     8041 ttggcatgta gcgatcgaaa caagcgataa agtggtccaa attgagagcg tcaacgcttt
     8101 cagcaatacg atcgtgacaa acaatactgt accaaaagtg gaagatcaat aaaaaagcga
     8161 gtataatgaa aaaagtttac aaagtaagaa gggaagtagg agtcaaatgg ctgaagaaaa
     8221 accagctttt aaaatcagta tcctagcaag tggaagttcg ggtaattcac tttatatcga
     8281 aagtgaaaaa aaacggcttt tggtcgatgc aggacttagc ggtaaaaaaa tcacttcctt
     8341 gatggctcaa attgatcgga caccggacca attagatgga atacttgtga cgcatgaaca
     8401 tcgagatcat atacatggcg tgggtgtctt ggctcgtaag tatcatttgg atgtttttgc
     8461 caatcagcag acttgggaag cgatggaacc actgattgga aatgtaccag tggaacaaaa
     8521 acatctattt gagatgggga aagtcagaac attcggcgat atcgatattg agagtttcgg
     8581 cgtctcccat gacgcagcgg ctccgcaatt ttatcgtttc cataaagaaa atcggtcatt
     8641 cgtcatgttg acggacaccg gatattgcag tgattatatg cgaggtgtca ttgaaaatgc
     8701 cgatggttat ttgatggaaa gtaatcatga tcttgagatg ttacgcatgg gaccttatcc
     8761 gtggaattta aaacagcgta ttttaggaga tagaggacat ctttccaacg aagacggtgc
     8821 acttgtcatg actgatgtga tcggtaatcg gaccaaacga atctatcttg gccatcttag
     8881 caaggaaaac aatatgaaag aacttgctca tttaacaatg gaaaatgttt tgaaagaaaa
     8941 agattttggc gttggacatg attttgaaat ctatgataca gatccagatg aagcaactga
     9001 gctattttcg atttaaacag ttcaatcaat aaaaatataa aaagcagaag ataaaatcaa
     9061 tcagggttgg ttttatcttc tatttttatt tttttagcat attaaaaaaa taaccgtatt
     9121 atcgactgac tacactgatg tgctgctttt catgtttctc atccagtatc tcatctacta
     9181 atgcaatcgc gtaatctgcc atgctgatcg tactgtcgct agcagcgttt ctttgtaaat
     9241 aatcatcact taacaaataa gtacctgtac gtttgccatg tggatcaaat aaagaagctg
     9301 gactgatata agtccaatgt agatttttag attgtttcaa ttttaacaat ccttcgtaca
     9361 tattgaaagc tgttggatag aagggtgcat ttttaggtac ttggtcaatc atacgagtcg
     9421 tcttttctgt atctaagaat aacgaagagg cacccccaac gacaattaag cgtgtatcgg
     9481 ttccttcaag catctcaatc aaatgggaca aagaagtttg atgcatttct tcttttcctt
     9541 tgggagcgtt gaaagcatca acgacaacat caaaagatgc aagatcagta gttgtcaact
     9601 caaataaatc cttttgtata aacggaacat ctgatggcaa tttttctgga tgacgcacga
     9661 ttgccgtcac gtctaaatct ctggacaatg cttcgtctaa aataaatgaa cctgcatggc
     9721 cagtagctcc aatgagtgca attttcataa aaaacctcct tttttattcc tgtccattgt
     9781 aagcagttgc tgaatattcg tcaaagaatc cgatttttga aaagctcagt gcgcattcct
     9841 ttcatggttc gtgatcattg ggtatagtta aattaagaaa actgattgat aaaaatagaa
     9901 gttgaaggag ataagaaatg aaagagtggt tagatcgtgc ctttttacca atgataacat
     9961 ctgttgtgaa tcttagaccc ttaactgctt tgcgtaatgg aatgacttac acgatcccaa
    10021 taattattgt tggctcgctg tttttcttat ttgctcattt tcctgtggat tacatcgctc
    10081 agtggataga caaaacagga ttttctatgt attttacaca agtataccag gctacatttg
    10141 atgtgatagc cttttggatc gtttttgcca ttgcatacgc ttatgcaaaa gaagaagaca
    10201 tcgatcgctt accagtcggc ataacagcct tgtctgctta tctactgctt attcagccac
    10261 taaatactca gatagatact gtctggacag gtgctgaagg gatactagga gcagttacaa
    10321 ctgggctcat catcggatgg ggctatgcac gtttagtaag acttgcgaaa gggtggaagc
    10381 aaagagagaa gatacctgaa aatgtttggc agtcttttac ttcattgatt ccaatcattg
    10441 ttatagtcac ttttacgctt atcggttcag cggtttttca acatttgttc agaatgactg
    10501 ttgttcaagg agtatggaca tatgtccagc ttcccattca atcgttcata gacacgttgt
    10561 ttggtgtgct tttattaggt ttttttgttc cgtttttatg gttatttggt cttcatggat
    10621 ctgcgatggt caatgggttg gttagtcccg tcttgcaagc gaattcatta gcaaatgcag
    10681 aaatcttagc ctcaggacaa gagttgacgg tggcaaatgg cggacatatc gttactcagc
    10741 aatttctcga tcagttcatg acggttacgg gtgctggatt gacgttaggg gctgtatttt
    10801 ttatgacggt ttttgcgaaa tcgaagaaat acagagaatt aggaaatctt gctcttttac
    10861 cagccttttt caacatcaat gaatcaatta ttttttccac accgatcgtg atgaacccga
    10921 tgatggctgt tccttttatt ttcgcaccgg tcgtttcagg attgattacc tattccgctt
    10981 tatattttgg attcgttcct ttatttacag cagtccaagt cccttggaca acaccgccga
    11041 tcctctcggg tttcttagta ggcggagtgc ctgcagcgat tttacaaggt atcgtgttgt
    11101 caattagttt tttcatttat ttcccatttt tgaaaaaaat agattctgca aactgcaaaa
    11161 aggaaagaca gacaaaaaat gatggagcag cagaaaagat gaacgactaa aaaacaaaaa
    11221 tactcctgtc gctttagatt gggaaaatca ccaatctagg atgacaggag tatttatagt
    11281 catgtttata ctagcccttt ttggacagct aattgaacaa gaacaactaa tgtgttcatt
    11341 cctacgtgtg tgatcatcga tgtccagatc cgaccagttg ctttgtactc taggctgaag
    11401 aagaaaccta ggaaaaagta gattagcaga tgaccatcat tatgagcaaa cgcaaacagc
    11461 aaagagctga tcactgctcc gacccaaaaa tttgaatatc gactgatgat ccccagtata
    11521 ctgcggcgga atacgaactc ttccatgatg ggtccagcaa cagttgtcgc caaaataaaa
    11581 gcgggtgctt ccatgatcat ctggatgata ttttgtgtat tctcggaagg ggttgctttg
    11641 ccaaacaata cagattctaa catgacagcg atgctttgca gaatcaatgc gacaaagatt
    11701 ccaaccaatc cataaataac aatctttgga gcagaagctt tttttaccgc tgattcgatc
    11761 gctaagggtt cacgttgctt aaaataaaaa atagctagga caacggcgcc tagtatatac
    11821 atgactgcag ttgctgtgat caattcagac gtagatttta caagtcctat cgaagctagg
    11881 atcaagggtg agataaaaat tagaccatag caaaaaatgc tgaggaagct atattttttt
    11941 aaagacatgt aattcctcct tttaagggat aacaagcatg caaggcaagc ttatatcgat
    12001 tgatttcaat tatatcataa tttcaataag cagctgtcag aataaaattt cttttctttg
    12061 atttaaaagc ttgcaaaaaa gagggagaat gagtattatt aaaaatgtag gttagcactc
    12121 gaaggttaag agtgctaaca gattaaaaaa agagttatcg gagggattta tcgtgttaaa
    12181 accattaggt gatcgcgtca ttatcgaagt cgcaaaagaa gaagaaaaaa ctgtcggagg
    12241 aatcgtttta gcttctgctg ctaaagagaa accccaaact gggacagttg ttgcagtcgg
    12301 tgaaggccgt ttgttagaaa acggtgaaaa agttcctgcc gctgtaaaag caggcgacca
    12361 agttatgttc gaaaaatacg ccggtacaga agtaaaatac gaaggaaaag aatatctgat
    12421 cgtcgcagga aaagatatca tggcgattgt tgagtaaatt tggaagataa aaaaataatc
    12481 aaaaataatc aatgaggtga atcaatcatg gcaaaagaat tgaaatttgc agaagacgca
    12541 cgcgcagcta tgttacgcgg ggtagataaa ttagcagata cagtgaaagt aacattagga
    12601 cctaaaggcc gaaatgtcgt actagaaaaa tcttacggct ctccattgat cacaaatgat
    12661 ggtgtgacga tcgcaaaaga aatcgaatta gaagatcatt ttgaaaacat gggtgcaaaa
    12721 cttgtatcag aagtagcatc aaaaacaaat gatattgcag gagacggtac aacgacagca
    12781 actgttctga cacaagcaat cgttcgcgaa ggtttaaaaa acgtaactgc aggtgctaat
    12841 cctctaggca ttcgtcgcgg gatcgaattg gcaacaaaag cagcagtcga agaattgcat
    12901 aatatctcaa ctgttgttga ttcaaaagaa gccattgcac aagtagcagc tgtttcttcg
    12961 ggttctgata aagttggaca tttgattgct gatgcaatgg aaaaagttgg aaacgacggt
    13021 gtcatcacaa tcgaagaatc aaaagggatc gaaacagaac tagatgttgt agaaggtatg
    13081 cagtttgatc gtggttactt atcacaatat atggtaacag ataacgacaa aatggaagct
    13141 gttctagaaa acccatatat cttgatcaca gacaaaaaaa tctctaacat ccaagatatt
    13201 ctgccattgt tagaacaaat cttgcaacaa tcacgtccat tgttgattat tgctgacgat
    13261 gtagatggtg aagcattgcc aactcttgta ttgaacaaaa tccgtggaac attcaacgta
    13321 gtagctgtaa aagctcctgg tttcggcgat cgtcgtaaag caatgcttga agatatcgct
    13381 attttgacag gtggtacagt aattactgac gatctaggtt tagaattgaa agatgcaaca
    13441 atcgaaaact taggaaatgc ttctaaagtt gttgtagata aagacaatac aacgatcgta
    13501 gaaggttctg gagaaaaaga agcaatcgaa gctcgtgtgc aattgatcaa aaaccaaatt
    13561 gctgaaacaa cttctgattt tgaccgcgaa aaattacaag aacgcttagc taaattagca
    13621 ggtggtgtag ctgttgttaa agttggtgca gctactgaaa cagaattgaa agaattaaaa
    13681 ttacgcattg aagatgcatt gaatgctaca cgtgccgcag tagaagaagg aatggtctca
    13741 ggtggtggta cagctctagt gaacgttatc agcaaagttt ctgctgtaga agcagaagga
    13801 gacgtggcaa caggtatcaa gatcgttgtt cgtgctttag aagaaccaat tcgtcaaatc
    13861 gctgaaaacg ctggttatga aggatcagtg attgttgata aattgaagaa cgtggaacta
    13921 ggcactggtt tcaatgctgc tactggagaa tgggtaaaca tggttgaagc aggtatcgta
    13981 gacccaacta aagtaacacg ttctgccttg caaaatgccg cttccgtttc tgccttgtta
    14041 ttaacgacag aagcagtcgt tgcagataaa ccagaaccag cagcgccagc agctcctgca
    14101 atggatccat caatgatggg cggtatgatg tagtaattaa gaaagaaccc tattttaaca
    14161 gggttctttc ttcttttttt gttttatttt aagcaaaagg ggcaaagaag gggcaaaaat
    14221 atcaaaggct atttagttta tcaattactt gttgtttggc tctttttgtt acatggttat
    14281 aaatagacag agttgtgttt gcatcagaat gccctacacg ctccataatg gctttaagag
    14341 gtacgcctaa ttcagataat agtgaaacgt ggctatgtct gaatatatgt gaggatagac
    14401 ttttttctaa ttccaactct tcctctactt tatggagtat agcgttaaat gagtgcagcg
    14461 caagcggagt gccacttgta gatataaata tatattgatc gggatctgtg ggtctacctg
    14521 caagaatatt gtcagctatc acactttcaa tcaattcttt tgcacgattg ggtaattgca
    14581 cttcacgttg cgaataagta ttttttggag ttgtttttat agcattatcc attttcacag
    14641 atgtgtaatc taaggtccca ttaatggaaa ttttcccatc ctcatagtcc ttcatttgca
    14701 aagctagcaa ttccccatat ctcaaaccag ttaaatatag aaactcagct attatgccgt
    14761 gtagttttct gcgaggattg gagtatagct gtttcagtat ttgatcaatt tcttctttct
    14821 ccagatattt tttattcata gaaagccttc ttttttcttc ttccactttt ttaggatgga
    14881 ttttaactgc tagcgcaggg tttctttgaa tgtattttct atcgattgca tagtttagca
    14941 taacagatag agttgttttt gtttgttttg tgtagttcag tgagaggtca ccaaacgtat
    15001 acatatcttc aattatctta ttaatgagtg tctcatcaat gtttctaacg atcgtatcat
    15061 cgcttatgtg cttagaaaca tgtttcatca tcattggaac cttcaaatag ctagtacgtt
    15121 taacatgctg cttataatat tcataccatt ctttatacag ctcaccaaaa gtgatatctg
    15181 atttattgta atcttcgagt gcttctttaa tttttttatc tagaattttc tgagcttttt
    15241 tccacgcttg tggtgaatta cttgtaagtg ttgtagattt ttttcgtgtt ttttctgtat
    15301 aaggatctac atatctttca ataaacttga attggccatc tttggtctgt tcaacccaca
    15361 cttttaacat ctcctatcat ttgctataat aggcataaca aatagaccta tataggtttg
    15421 ttttctaaaa gcacgctctt actctggacg gtggggcgtg ttttttattt attatacagt
    15481 tgctttaaaa atagctgctt tttcaaatga tactaaagga gagaagtgga tttctatttc
    15541 tcccttttgg tttaacgtga aatgagcagt tacatccatt tgttttcctg gagcaacaga
    15601 tcccattgta ttttcgttgg catatgtctc tgattttttg tcatctggtc catatacttc
    15661 cacatctgta cctacaggga tatctgaatc gccatcattt tttacggtat aagtaatttt
    15721 tactacttgt gcaggttggt tttcttcaaa ttgatttctt tcatcagtta gttctacact
    15781 atttagtgta tattcagcat caccaacttt tacagtgtca ccaatcttat agaaggtatc
    15841 gcttttttct tctttagaag atgaagcaga ggttgaagat ttagttactt tttcgccacc
    15901 attatcactt gctttattac tatctgatcc accatttaaa gcagaaccaa taattataat
    15961 taaaattact gctaataccc aaaaccatac ttttttgtaa aaaggtttac taactttata
    16021 catttttccg tcttgaccca taactttttt tgccatttaa atattcctca cttcttgtta
    16081 taatatattt gcgatctcag aaatgaggta tgagtccgtg tgccagcacg ggctttttta
    16141 atgttttgct gaaatcggtt tttttaataa ttcttgatgt attcatacat attgccttgc
    16201 gtaagaatat tttttcttaa aatagcattg gcagacagca taacaagtgt atctgagctt
    16261 attctgaata gaataatact ccataaattt ttcgagatta aactgagatt catcagtcag
    16321 ttcgttctca atatagatat ttaataaaat tagaatagct attttatctg cttctgtttc
    16381 aaattttgag tgaaaagttg tagaagtatc gtacaaaact gaatattcaa aatgagaagc
    16441 ggtgaaatgc gcaagttcat gagataaatg aaaggcttct gcagtttcgc catatagatt
    16501 ttcattcaaa aaaatgattc taggcttcgg ataatagaag ccaggctctt tcatttccat
    16561 gtaaactact ttcaaattgt attcgctcaa catttctttc aatttcaaat acatacaacc
    16621 catcactcca actattcatt ttcctctaaa gctttagcaa ttgcaatcgc tttacgcatt
    16681 gtctccttag atatttcttt tccatcaaaa gaaaaaacag tatcgttttc tgataaatcc
    16741 acatgtttag gggtttctct ttcttctcta cctagaaggt agtctacaga gactccaaag
    16801 taatcagcta accgagctaa ctcaatagaa ttaggagttt gtcttttcca acttcctaaa
    16861 tatccattcg aatacccgaa attcatttcg agctgtctaa tggataatcc tttttctttt
    16921 gctaactcct ttattatttc gtaagtattc attgataaat caacctttct gaatgcttac
    16981 aaaaaaagtt tagataaata atgtaaaaat agttgactta tatacattat taatctatac
    17041 tatgttttgt aaacaagttt aacaactaaa aagacaacaa aaaatactat tgattaataa
    17101 atgccaaccg ccaagaaagc tttttaaatc aaagtttata tgtcttattt aactatgcat
    17161 ttagtataga ttattaatct aataaagtca acgcagtttt ttaaaaagtt gttaaatttg
    17221 tttacgaata taaaagaaag gagagaagaa tatggaaaaa acagtctcgg caaaaatcga
    17281 agatttgaaa ttagacatac tgaaacaagc aaaagtggcg atggaacacg cggtagataa
    17341 agaagactct gccatggttg cagccatagc agagatttta gctcacgttt agtcatcgtt
    17401 tttacggtct tctggaagct gaccatagat aacagcataa tgaatatcta aataagcttg
    17461 aacaatttct tttggcgaaa ttgattcgcc tttagtagat acttgtgatt cgtgatttag
    17521 ccatgcaaca actaaatccg cagcgatttc aaacggcgtt acactttgtg tcagttgcca
    17581 taaagaattt cacaatatgt atggtcgtgg taataatacc aaagagcaat ttgaggaatt
    17641 tatggaaatt aaaaaataca aaaaatcatt tattaaagga ggctaaacaa tgaacaagga
    17701 aatgagccaa acaatcaagg tacaaaaaat gatagatgat ctaacacatg gaattgatag
    17761 ccaagccgat aaaatcatca aggaattgca aggacaaaaa gttaaggatg caaaaatgct
    17821 tctcaagact atcaactttg aaatgaatcc aacaaaaaga aagctcgccg atgtgttgga
    17881 agaaaagttg gcttccgcta tcaacgagca agaattacta tttgaaaccg atacttttaa
    17941 cagctgatgt tttatagatg acagtttcat ccgaatctat atcatcgccg agagaaaaga
    18001 atgttgcttt tgacaaaaag ccgataagac ctacaagcgg tcttgatgtt gcaagagtag
    18061 ataccccaat atcaccttgc aaggtattca cgtaaatttg agaagaatag aacattccat
    18121 ctttttcttc gatatttgac gcatcatcgt atccaacgac caaagtatcg gcaaatattt
    18181 tgattttttc tccatcaagc atagtaatta caaattctgg tttcataaat tttcacctcc
    18241 ttaacaatta tttcagcctg tcacactgat aaggaaatta taccaaagaa aggaatgaga
    18301 aatatgaata caccacaaat ttttaatttc gagcaaaacg aagttcgaac tattttagta
    18361 aatgatgaac catattttgt aggaaaagat gtagcagacg ttctaggata ttcgaatccg
    18421 caaaaagcta ttcgagatca tgtggacttg gaggataaga cgcagaacga ttcgttcacc
    18481 gtcaatggaa cagcagttgt tttgattaac gagtcaggcc tttacagttt aatcttaaaa
    18541 tcaaaacttc cctctgccaa aaaatttaaa cgttgggtaa cgagtgaagt ccttccaaca
    18601 attagaaaaa caggtagcta ttcaaacgta cctcaaagtt ttgcacaagc attgcgttta
    18661 gcagcggatt tagaagaaaa gaaccaatta ctcgaacaac aaattgccga gtacgaacca
    18721 aagattagct acttagatac gattctttca tcgacagata cggtagctac ttctcaaatt
    18781 gcagctgatt acggaatgtc ggcaattgct ctaaacaaat tgctaaacga gttaggtgtt
    18841 cagcataaag ttagcggaca atggatactt taccgaaaac atatgaacca aggatacaca
    18901 aaatcgcaca caagtgagat accgaaagcc gatggcggca ctaaagttgt aatgaatacc
    18961 aaatggacac agaaagggcg agtgtttatt tacaacttat taatcgcaga gggctattac
    19021 cctcaaatgg atttagagga aattggttag aaaggagttt tagtatgact gacattgcag
    19081 aaatcactca acgagataga gaaaaaatca aagaatatgt cgaaagttca aagttcttaa
    19141 cttacaccat gcttgctgaa agatttggaa ttagcaaaag ctacttatct ttaattttaa
    19201 acggtaaaaa gacttctgca gaagcaaaca gaattataga ttcgattatc actatgtacg
    19261 aattgtagag ggaggtaaag tcgttggaag ataaaatcat tcttacaaaa tcagagcttc
    19321 aagaaatgtt aaacatcgag tatggaacga taatttttag gagggataat aagtgaaaaa
    19381 accaacactt tcagagctga tagaagctgc tgagaaggca gtaaaaccag acgactggta
    19441 ccgacaaagt ttaatcttgg agaagttcca cggcatgtca aaaactactt tagttgaata
    19501 ctgcaaggaa atggaaacaa ttcctgaatt tgcagaagga atacttcgtc caggtcactc
    19561 aaccacattt attcattacc atacttttat ttggttttta aagtggaaag acgcaaacaa
    19621 atatcgtgta aaaagtttat cgccttcaga tgttttgaag gaggcaagtt aatgggcaaa
    19681 ttcaacagag cactagtatt cagcgcaccg ctaatcatct acgctttagg cctctgggga
    19741 agcagacaag cgttgatagg gacaatcgtt tacatggttt ggatttttat ggggcttgat
    19801 gaagctgagc acagagcgaa aaaagccagt cgggagggac tgactaaaat atgatttttt
    19861 tatttttgtt cctttgggct gctattgcgt acttcatcca gctccttgtt gtcaaagttg
    19921 ctcttatttc ttttaggaag aacaaaagaa aatagcgcta taaatgaaag ataagtagcc
    19981 gctacattaa actgttcttt tgaaaaagtt acagtcatcg gaatgataat tgatattgag
    20041 gaagtaatct taattaaaaa tatagcaata ttttcaacat cgcgttggtt tttatttaac
    20101 attgtccaac atgttttagc aacaataaaa ataattacac atgatattag cacacagact
    20161 gaaaaaagtt ttagaaatgc gatgtagtca gtgttattga actccttcaa aatactattt
    20221 ttattcaatg taataaaagg aattttttta attgaagaca ggaaagataa ggtaataaac
    20281 agaattatat atatggttaa agtcgcagta agaatgtaat tgagcttttt cctcatagaa
    20341 tcacctcact ttctaacgaa aattatacca aaaaggagag aagaaataat gcaagaatta
    20401 gtaattttga aaaataaaga agctgtaact acgagcttac aagtggcaga aagtttcaaa
    20461 aagaaacata agcatgtact agaagcaatt gaatcaataa aaagatcggt cgaaaattcg
    20521 gccaatgttg aagatgggtc caattttgga cagatgtttg tggaagggaa cgagccagac
    20581 tcatacggaa gaagtcggag agtttatttc atgaatagag acggattttc cttgctagct
    20641 atgggattca ctggaagtaa agcaataaat ttcaaactaa aatttattga agctttcaac
    20701 gaaatggaag atgttattcg gaagaatact gttcctcaaa caattgaaga catgatgatc
    20761 tatcaattag aagaaatgaa agatgttaaa aaagatgttt ccatgcttaa agatactatg
    20821 cgaattagcg gacaacaaga gtttgaaatt aagcaaaaag gaaatatgaa agttatggaa
    20881 gttctagggg gaaaagaaag ccgagcttat gaagaaatca gcaaaaaagt attctcaaaa
    20941 ttttggtctg aatttaaacg taccttttca atcccaagat atggcgagtt acctcgtaag
    21001 agattcgatg atgctgtttc atttattgaa atgtggttac cagaaactgc gatccgcatg
    21061 gaaatcgatc aactgaacag acaacaaaga cttttcggtg atgaaaatga atagagctga
    21121 agcgctaaga atagggacgg taattgctaa tcgctggtgg agacacaata aaccaaacat
    21181 cctaagccaa caacatatcg aaaagcaaaa agcatggcaa caaataaaaa agtgactcag
    21241 ccggcaagca tagagtcaca aaacatatct aaggagaatt ttagcatatg aataaagaac
    21301 tttccacttt agatcaatat ttgactgatt ctgaatgggg caagtcgaat atcaaggaaa
    21361 caaataatcg aaaaatcaga cgaaatcttt tgacgaacga agaactatca tgtgatcaag
    21421 atgacttggg aaattttgtg agtatttggg atcatgttta ccttatccat ctatcaaaac
    21481 attcaaataa acctgaatac atctatgtca tcgaagatgg cttgattgat gcgctagaag
    21541 agtacaacag agataacttg attgatatct cttattacgg atcaggtaag aaatacattg
    21601 ctgaaatgga ggcagaattt gatgagtgaa atcaaaggga caacgaactt tgaaaaactt
    21661 tttagtcgta agttaaataa aattctcaag aaaaaaggaa attttgatta tttatcttgg
    21721 gctcacgcgt gggagattat gaaaaagaat gatccacagg caacggtaac tattaatgag
    21781 tataaacact acagggttgt ttctggaact catcaagact ttcttgttga ggaatataaa
    21841 ccttttctta tggatgaaac agggacttat gtatctgtct cagtaacggt taaaggacat
    21901 acggaaacag a
//
LOCUS       DAZUKZ010000038        21496 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000038 DAZUKZ010000000
VERSION     DAZUKZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.038"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            177..878
                     /locus_tag="ACG8EF_001391"
     CDS             177..878
                     /locus_tag="ACG8EF_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4946705.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1487..2503)
                     /gene="tsaD"
                     /locus_tag="ACG8EF_001392"
     CDS             complement(1487..2503)
                     /gene="tsaD"
                     /locus_tag="ACG8EF_001392"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFS4946706.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2500..2964)
                     /gene="rimI"
                     /locus_tag="ACG8EF_001393"
     CDS             complement(2500..2964)
                     /gene="rimI"
                     /locus_tag="ACG8EF_001393"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS4946707.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2971..3513)
                     /gene="rimI"
                     /locus_tag="ACG8EF_001394"
     CDS             complement(2971..3513)
                     /gene="rimI"
                     /locus_tag="ACG8EF_001394"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS4946708.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3497..4219)
                     /gene="tsaB"
                     /locus_tag="ACG8EF_001395"
     CDS             complement(3497..4219)
                     /gene="tsaB"
                     /locus_tag="ACG8EF_001395"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFS4946709.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4410..5390)
                     /locus_tag="ACG8EF_001396"
     CDS             complement(4410..5390)
                     /locus_tag="ACG8EF_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4946710.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5414..6898)
                     /gene="galT"
                     /locus_tag="ACG8EF_001397"
     CDS             complement(5414..6898)
                     /gene="galT"
                     /locus_tag="ACG8EF_001397"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFS4946711.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6910..7899)
                     /gene="galE"
                     /locus_tag="ACG8EF_001398"
     CDS             complement(6910..7899)
                     /gene="galE"
                     /locus_tag="ACG8EF_001398"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFS4946712.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8147..8314
                     /locus_tag="ACG8EF_001399"
     CDS             8147..8314
                     /locus_tag="ACG8EF_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFS4946713.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8376..10187)
                     /locus_tag="ACG8EF_001400"
     CDS             complement(8376..10187)
                     /locus_tag="ACG8EF_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFS4946714.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10184..10549)
                     /locus_tag="ACG8EF_001401"
     CDS             complement(10184..10549)
                     /locus_tag="ACG8EF_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFS4946715.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10712..11107)
                     /locus_tag="ACG8EF_001402"
     CDS             complement(10712..11107)
                     /locus_tag="ACG8EF_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4946716.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11125..12087)
                     /locus_tag="ACG8EF_001403"
     CDS             complement(11125..12087)
                     /locus_tag="ACG8EF_001403"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFS4946717.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12087..12299)
                     /locus_tag="ACG8EF_001404"
     CDS             complement(12087..12299)
                     /locus_tag="ACG8EF_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFS4946718.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12320..13018)
                     /locus_tag="ACG8EF_001405"
     CDS             complement(12320..13018)
                     /locus_tag="ACG8EF_001405"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS4946719.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(13038..13580)
                     /locus_tag="ACG8EF_001406"
     CDS             complement(13038..13580)
                     /locus_tag="ACG8EF_001406"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFS4946720.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13712..14716)
                     /locus_tag="ACG8EF_001407"
     CDS             complement(13712..14716)
                     /locus_tag="ACG8EF_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS4946721.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14713..15702)
                     /locus_tag="ACG8EF_001408"
     CDS             complement(14713..15702)
                     /locus_tag="ACG8EF_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS4946722.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15699..16505)
                     /locus_tag="ACG8EF_001409"
     CDS             complement(15699..16505)
                     /locus_tag="ACG8EF_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4946723.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16671..17627
                     /locus_tag="ACG8EF_001410"
     CDS             16671..17627
                     /locus_tag="ACG8EF_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4946724.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17704..18222)
                     /locus_tag="ACG8EF_001411"
     CDS             complement(17704..18222)
                     /locus_tag="ACG8EF_001411"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS4946725.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18310..18459)
                     /gene="rpmG"
                     /locus_tag="ACG8EF_001412"
     CDS             complement(18310..18459)
                     /gene="rpmG"
                     /locus_tag="ACG8EF_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4946726.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18687..19133
                     /locus_tag="ACG8EF_001413"
     CDS             18687..19133
                     /locus_tag="ACG8EF_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFS4946727.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19328..21223)
                     /gene="asnB"
                     /locus_tag="ACG8EF_001414"
     CDS             complement(19328..21223)
                     /gene="asnB"
                     /locus_tag="ACG8EF_001414"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFS4946728.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6473 a         4561 c         3701 g         6761 t
ORIGIN      
        1 cacaaatcat tcgcttcaaa acaaattatt caataccatt gtatattttt tacataataa
       61 caaattatat ttgtaaagta ttaaaacttg tttctaatgt tggtatgtta aaactttaca
      121 aattctttaa aaaaagtgac aataaatata gtgaacaata aggagaggac tttttaatgc
      181 gtgttaataa aatcgatttt cacttttcaa aactcagata tcaacctgat tttgaacatt
      241 tctcggatga agagttccaa cagatcaagg aacatactgt tcttcgttct tataaaaaag
      301 gacagatcct tttcgacgat ggtgatccca gacaacggtt gtatatcttg accaaaggac
      361 tcgttcgttt agaacgttat gatgaaagtg caacttatta ctactatgac tatgtgaata
      421 caaataacct ttttcctatg tcaggaatgt ttggagaaga gacctacact tatacagctc
      481 aagcgatgac tgatattgaa acattctacc ttcctgtttc ctactatgaa aaattagcac
      541 gaaaaaatcc cgaacagttg atctatatga tacaaaaaat ttctaaagta gttgaaatac
      601 atgaattgcg tatccaaatc ggattgacct ctagcgcctt tgaccgggta aaacaaaatt
      661 tattcatttt gaaagaagaa ttgggcgtac caacaaaaga tggtcatatc accattcctt
      721 atccgatcac cttaaaagaa ttagcagtga atagcggcac gacacgtgaa actgctggac
      781 aagtaatcaa acaactacgt gattccgggc tgttagatta tgacaaaaag cgttttactt
      841 tctgtaagac agctactact ccagctatga gagaataacg cgaacaaaaa taagcagtga
      901 ccctgataaa tatcagggtc actgcttatt ttcctcccta aaacttctga aagatccggc
      961 agttttagat cactggacaa gactcaaaga ttttctatgt gtgtttcagt tatgtaatat
     1021 tcactgtttg caaaaaatac tgttagatta actagatgaa gcgtcttgtc ctcatccgat
     1081 ctagattatt cgatattcct cgacaataaa cgattactca gtgagggcaa tcagaggaac
     1141 ggataatgat aaataagctg taaaaaatag caaaataaca ataatgatta aaagtatgta
     1201 aaaaataggg ctttgacaaa aacgtgtcac agccctattt cgattaaacg gtgtgtacct
     1261 gtcaactctt cggtcacaag tcataatgtt cataaacatg aggagctgtt ttttgaaatt
     1321 gttccttgtg aatcagagtt aaacgacagg cttacgaact cttgattaaa cggcgttcaa
     1381 aagcttgcat acgcggtaat aagaacagcc aggtcaaaaa catgagaagc cgtttttcct
     1441 gggctgttct tattacttga tgcagggcag ctttttcaca acctctttat tcagataaaa
     1501 ccaagcttgg atcagcattc aactgatagc tagcaaaatt tcttttttgc atttccacat
     1561 gtgctgctgc tccaatcatt gcagcattat ctccacaaag tcgaagaggg ggaatcacta
     1621 gttctacctc agggagttta gctgccaaag catcctgcaa tccttcacgc aacccttgat
     1681 tggccgctac acctccagct ataatcagtt gcttgactgg ataattttga caagctcgaa
     1741 gtgttttagt aatcaataca tcgatcacac ttgcttgaaa gctagctgct aaatcatttt
     1801 tgtctagatc ttctccacgc tgttgtgcat tatgcaccaa attgataaat gcacttttta
     1861 atccgctaaa actgaaatca taattatctt catggatcat cgctcgcgga aaatggtaat
     1921 tatcttttcc ctgatgagct agctgatcga tttcttttcc gcttgggtaa gaaagaccta
     1981 atactcttcc aactttgtca taagcttctc ctgctgcgtc atctcttgtc tccccaatga
     2041 tttcatatga tccatcttct tgcatataga ctaattctgt atgtcctccg ctaacaagaa
     2101 gtgccatcaa tggaaactgg aatggtttaa ccagacgggc tgcatagata tgccctgcca
     2161 tatggtttac aggaataagc ggcaattggt gtgcccaagc aaaagcctta gctgcagaaa
     2221 taccaatcag caacgagccg acaagtcctg gtccataagt gacagcgact gccgataaat
     2281 cttctgccga cacaccagct tctaccagtg catcttccaa gcataaggtg atttgttcta
     2341 cgtgatggcg gctagctact tcaggtacta cgccaccaaa acgtttatga ctgtttattt
     2401 gagaagcaac gatgttagat aagatctctg ttccatttct aacgaccgct acactcgttt
     2461 catcacaact tgattcaatc gccagaatca attcttcatt catttcatcg tctccgtttt
     2521 taactttctg ctcataatga tcgcatcttc cgtcggattg cgataatatg atttccgttt
     2581 acctaatata cgaaactttt ctgattcata aagttttctt gctgcttcat tagatagacg
     2641 tacttctaaa aaaacatgca gcgaatcttt tatttgttcc gtaatcagta attgtaacaa
     2701 atgccgtgca tgcccttttc cttgttctga aactgcaaca gcaatatttg tgatctcgac
     2761 ttcatctatt actttcgagt atccaataaa gccacagatc tggttatctt cagctaaaag
     2821 cagataatca gagtgaggct gttggatgtc tgccaaaaac tgcattttcg tccaaggtga
     2881 accatgtgga taagcatttt cgctaatcat ccatacgcat tctgccaagt acgcttcatc
     2941 aaaatctttt tttctgcaaa tcaactaatc tcactcattt tctaaaagtt ttatcatatc
     3001 caacgcatct tcttttgttt cagtgtaata tctttttttg atcgttgttg cttgaaaccc
     3061 caatttccga taaagtcttt gtgcatcttg attactcata cgcacttcta atgacatcgt
     3121 ctcacagcga ttcattatag caaagttctc gatttcgtca ataagcagcg aaccgattcg
     3181 tttacgctga tatgccggtc gaacggcaat attcgtgata tggcaatcta aaccgaacgc
     3241 tcttgaaccg ataaaaccaa tcattcttcc attatcaaag atacctagat atagatgagg
     3301 aagcggtgac ttcaattctg atataaaggc actttttgtc catggcaatt cgccattata
     3361 gacttctcgt tcgacatcca gcagctcttt gatatcttct gtagtcaatt ctcttagtac
     3421 gtaacgacga tcgatttgtt cgactgtttt ttctgtataa atcattttat cttgaaaaaa
     3481 gcttctggca tacgctctaa attttttcaa cataactttc gtctccaggt gtgtgcgtct
     3541 ccaaccattt ttcttctgct tctactcgtt ttaaatagcg aggaagaaaa gcttgtttct
     3601 ctgcaactgg ttcaactaaa gcacctaatt gagccacagt tacaccactt ggaatatccc
     3661 atgctggaac tgtattaatc atcgcactag gcaatatttc ctttatttca gagaagaatt
     3721 tgtgacaatc actcccgacg aaataaatag cttcattcag cttttgtagt tgtgtgaaca
     3781 aatcagaagc agaaatatgt acatcttcaa gtacagtttc taatctattt tctttccagc
     3841 ggtatgcccc agcgtataca ttttttcttc gagcatcaaa catgggcaca ataatattat
     3901 ccacaccgat gcaattagcc gcgatggttt tcagactaga tactcctact agctctttat
     3961 ctaatgtata agcaatcgtt tttgctgtcg ttacacctat acgaagccct gtataagaac
     4021 cgggaccatc agaaaccgcg attcggtcaa tgtcttttgg acttatttga agatctgcaa
     4081 acaattggtc gattgcaggc atcagtgtta cactgtggtt gcgtttgata tttgtttgta
     4141 tttgtccaag aatttgttcg tcttctacca caccgatcgc caaagtttgg tttgcagtat
     4201 ctattcctaa tgtaatcata tatttgatcc tttctgctat gacattctcg ccttccattg
     4261 tagcatactt ttttattctg aggatgaaaa taccaaaggc aaaactagta caataataca
     4321 tgattatctg ctggcgggca aattttgccc cttcctgaaa cacaaaaaag ctgcggtatc
     4381 actccatttt cccacagctt ttccatcttt tatttcgttg attctcgaat aatcaactct
     4441 gtttccacaa taatctttct tgaaaccggt gctttggaat tacttaattg aagaatcgtc
     4501 tccactgcta gctctcccat ccattcggta taaactttta cagtagaaag cgagggcgtt
     4561 acatatttcg ctacactgac atcattgaag cccatcacag aaatatcttc tggtaccact
     4621 aaaccttttt cttgaatcgc tttcagtgcg ccaatcgcta atgcatcatt agctgcaaaa
     4681 agcgcatcag gaacatgcgg attcgttttt aaatattgct tcactgcttg atatcctgac
     4741 tcgacagaaa aatctgcttc gataattgga ccttcgtcgt agcctttttc tgccaaaact
     4801 tgttgaaatg ccataattct gggatctttt agccgctgat ggtttttctt cgtatattct
     4861 gttccagcaa gcatagcgat atgttgcgct ttatgggaga caaagtgttc aatgactttt
     4921 tgaacacttg acttaaagtc gacaactaga gagtcgattc cttgagaagt gccgtcagaa
     4981 tctaccaaaa gcaaggcatt ttgggatcct ttcatttcta ataactgagc ttcgtcgaat
     5041 tttcctagtg cgatcgttcc atctgccgga agatcactaa tggaatccca cgtttctttc
     5101 aataaagtaa cgtttgcttc ttctgccttt ttttcgattc ctaggcgaat agataaataa
     5161 taaagatctt caagctcttc cgtatcgtcg taccactgga tcaggcgaag cgtttgctta
     5221 gtatttgctg catttttacg atgtttcgta taatttaagc gttctgctgc ttcaaaaatt
     5281 ttttgctttg tggctaagcc aacggaaaga tcaggatcat agttgagaac tcttgaaaca
     5341 gtagcaggag aaactcctgc caactgcgca atgtctttaa ttgttgccat ttgtctctcc
     5401 tcctttttta cgtttataaa atcgcagtaa atcgttcaaa cgctgcctgt cctttttcat
     5461 ctcgcttgaa tactccagcg tcttccagta cacgtgcaaa aacctgacct acagattttt
     5521 gtacgacatc ggtgaccgta tctttcgtca aatcaggatg ttcttttttt aattgttctg
     5581 cccacggacg atgatattca gccatctggt tttcttctcc taataagaaa tgctctactt
     5641 ctactagttc tggtttcaaa cgaggtggta aaacagcaag ccccattact tcgatcaacc
     5701 caatgttctc ctttttgata tgttggacat ctttatgtgg atgaaaaatc ccatcaggat
     5761 gttcttctga aacattgttg tctcgaagga caaggtccaa ctcaaagtaa ttgtcccttc
     5821 ttcgtgcaat tggcgtaatc gtatggtgtg gtgtaccatc tgctgaaaaa gctctgatag
     5881 atactgtttc atccgaatat gctttccatt tttccaatac tgagttagct gcttcaataa
     5941 gcaagttttt ctctgttgat tgcaaacgga tcacagacat cggccatttc acgactcctg
     6001 cgatgacttg cggataatct tctagtttga taagtttttc tatcggtgct tttgccatag
     6061 gaaattcatg acgtcctgcc tgataatgat catgagaaag aatcgatcct ccaacaattg
     6121 gtaagtcagc attcgaacca acaaagtaat gaggcaagac ttcagtaatc cgaagcagtc
     6181 tagcaaatgt ttcctttgtg attttcatcg ggcggtgttg ttctgataaa atgatcgaat
     6241 gttcattgta ataagcataa ggggaatatt ggaatcccca gctttcaccg tctagattca
     6301 ttcgaataat ccggtgattg gtgcgggctg gatagttcaa gcgacctcta tatccttcgt
     6361 tttccatgca aagcatgcag gtaggatagt ctacttttac cgcctcgcgt tctgctgcga
     6421 tttgttttgg atctttttct ggcttagata aattgattgt gatttccagt tcaccatact
     6481 ccgactctgc cggaaatttg atgttttttg cgatggctct tgttttgatg tagtcatttt
     6541 tcttacttaa attaaagaaa tactcagttg cttcctctgg gcttttcgaa tagtgctgtg
     6601 caaagaatgc attgacgact gaaggtggcg gtgtgagaaa atccattaat tgcgcttcaa
     6661 aaatctcttg ttctgctggc aattctgaaa gaacaccatt ttctcttgcc ttagcgacaa
     6721 gctgatccaa taagtctaca gattcttcct taactggtct tacttcaatg ctttctagag
     6781 catcttctcc taccatccct aaaagacgat tctgtaaata taagcggtcc atttccatcc
     6841 atccgccagc ttcgatcgca agtgtggcaa aatcacaaat cgtttgactg attgacatat
     6901 ctcattcccc tattcttcat aaccatttgg atggctgaca tgccaatccc atgctgtttt
     6961 gataatcgct ttgatatctg tgtattcagg tttccagccc agaatctctc tcgctttttc
     7021 acttgaagcg actaatcggc taggatctcc agctcttctt ggtgctattt ctgctggaat
     7081 ttcttttcct gtcacttctc tagctgcttc tagcatttcc ttgactgagt atcctttgct
     7141 gctgcctaaa ttgaagaagt tgctttcatt tccttctttc aagtattcca aagctaaaat
     7201 atgtgcagca atcaaatctt ctacttgaac ataatctctg atacatgttc catctggtgt
     7261 atcataatcg tctccataaa cagccaaggc ttttctttga ccaagagcta cttgtaaaat
     7321 gattgggaca agatgcgttt ctggtgtatg atcttctccg atagatgcat ctgcttttgc
     7381 tccggctaca ttgaagtagc gcaaagcgac atagcgcatg ccatatgctt ggtcacacca
     7441 cttcatcatt ttttccatca tcaatttact ttcaccatac ggatttttag gattagctgg
     7501 tgtatcttct gtgattggag actctttcgg ctctccataa gttgctgcag tagaagagaa
     7561 tacgatttta ttcacgttga actcgtgcat gacttcaagt aagatttgca taccgtatac
     7621 gttgttgttg aaatacatca atggtttttc gacagattct cctactaaag agcttgcagc
     7681 aaaatggatt actccttcaa tcggttcctt ttcaaatacg gaacgtagaa attctttatc
     7741 ccggatgtct ccttcataaa aatgagcatc tggatgtact gcttgtttgt gacctgtgag
     7801 caaattatct accacagcta cttgataccc cttttgaact agctggtcta cagcgtgcga
     7861 accgatataa ccggctccgc ctagtactaa aattgtcata aaaaacactc ctttgattag
     7921 taaaattgtg tttgttgatt ctatataatt taaaaaacga agtttacttt tatggtgaaa
     7981 acaaacaaag taaaatttcg taaattcata tggtaaattt ataataaaac atttagtaaa
     8041 cattttcaag ggttttttgc aaaggtttac aaacaaacgt aaaactagcc tttttttctc
     8101 ttttgttcca taatagatat aacaaataat aaatgaggtg gattcaatga aaaaatttgt
     8161 atctggaata ttagtaggaa gtctggcaac agccgctgcc gttgctggtt tggttgcttc
     8221 tgtgaaaaaa acagtgatcg atccaattga cgaaaaagaa gcaatgatcg aagaaaaccg
     8281 taaaaaagca atgcgtaaac gcgtttctcg ttaataatta tttaaaaata cgcatgaaga
     8341 ctttctaaaa aacagataag tcatcatgcg tatttttatt tgtcagtcaa tataagttgt
     8401 gctattttat ctgcctgtct cagattccat ggatctgtat aaggtctagc tactgctgca
     8461 gttagccaat ccacaccttc aacaggtatt ccatagcaat aaaagtcaga ctggatttcc
     8521 cctgaagcat caatcacgcg attcgtacct cgctcaacat ctatagcttc tgaatgataa
     8581 cttcctgtat tatctgaaaa tgaaaaaggg cgaacaagtt tgttttcctt caaatttttt
     8641 atgacaggat tacttgaacg agaaaagctt gtttgtggca atcgagcttc aatcaaatag
     8701 cgactgctga tttctttatc ttgtagagaa ataacatagc ggttgtgctt ttgctgaata
     8761 tcaaatggcg gttcgatcaa atgaaaaata cccgcatcta tcagtgctgc taattctttc
     8821 gtccgaatga ttggcggacc gatcgttaaa aagctattta gatgagtaaa ccatccccat
     8881 agtatttctt tgtattcctt agcagaaaaa acgtgccatt caatagcctg ccggattgga
     8941 tcacgccagt cttttaatgc atcaaatgtt gaagcaaatg ctcctgtgca attgcctttt
     9001 tccgcttctt ttatctgccc ttcaataaat attcggctag cttctgaaaa gggctcattc
     9061 tgataggcaa gaggagtttc taaatacgac caatcccatc tataagggat tagtgctgga
     9121 tgctttccga gacactcctc tgaagagagg gagagaaaat catgctcaaa atttttcttt
     9181 ggaatcggca agtgatattc ttcgatgatt ttcttgtaat aaaacaactc aacgtctttt
     9241 ttcagatagt caaaaaatgt ttcgccagtc aaaaatcccg actgatgcca ttgctccagg
     9301 ttttcttttg ttagtaaccg tggccatgcc attgctccgc cttgcttttg atttcttcct
     9361 cgagggtaat aagggagccc ccttcttgat ccactgtata caatcggttc cttgcctgat
     9421 ggatgataga ccagttttcc cttttcgttc tcgaagcgcc ctcctcttga tactgtaaac
     9481 aatccgacat aatcaaaaaa acttaagcct agacctctta aaaagacatt ttctttaggc
     9541 ggtatcttcg aaagatctac atctgcagga ttagaaggaa gctgatacag taaaacattt
     9601 ttctttgcgt agtctaccca cactctttct tcttctgtct catcattttc ccagtgaccg
     9661 cttgctaaaa caagctgatc gatagaatag ctttgctgat ctgtctgtaa aagataacca
     9721 tcttttcttt tgcttacttt ttttactaaa gaataaacaa actgaataga agctggaaat
     9781 tcttcttgca actttgagaa aaaccagcgt tgatagagtc cgtataaaca tcgagtagat
     9841 tgctgatctt tttccagacg attgatttct tctataatta gttgcttatt tttggttacc
     9901 gtctgttcga tataatctgt agcaggtcct ttactccatt gaaacaagtt gggtccctgt
     9961 gttaccaatc ctccactgga caatgtttca tctgtaaaaa gcgtcacttg ttgagaaaca
    10021 gaattcatta ataactccgg tggctgattc aaccgccaaa ctcgacctcc aggaccattg
    10081 ggatcgaaaa gaaaaagttc aagagaatgt ctgtcctgat tattatggag aatcctttct
    10141 gctacactta atcctctagg tccggcacca atgatcccta ttttcatttg catgcctcca
    10201 tttcgataaa ttttttcaat aaaacagcat ggttctgatg ttcatttttt gcaccataaa
    10261 caaaagtaat gactggatgt tctttcataa gttgactgag tttaaaaata gcttgcttct
    10321 tttcttcttc gaaaatcaat tcttgctggt atttctttgc aaatgaagca aatcgttctg
    10381 gtacatggtc aaaagttttg cgtgcttcat tactaggtgc aatttctttc aaccacagat
    10441 ctaactgcgc tttttctttt gaaattcctc gtggccagaa tctatccacg agtattctat
    10501 agccatcctc tttttcagca tcaaggtaaa cacgttttaa tttgatcata gttgtttcct
    10561 tctttctacc ttttcgctta gcttatatat gtagataata gcacaaaata aaaaaaatga
    10621 acggcacaac tatccattgc gccgttcatg tgatcttggt caaaatctaa tgattgattc
    10681 ggtttttaaa gctgtcatac aatcatctgt tttatacttt tttacttttg atttttccag
    10741 accatccgtc atatccaccc ttaagtaaat aaatctcttg ataacctgct ttacgtaatt
    10801 tatttgctgt acggatgctc aaacttttct tttgatcgta aatatagaca ggctggtctt
    10861 ttcgaataga tcctaatgaa tctttcaaaa cagtgtaagg gatgtttctt gcacctaaaa
    10921 tatgcccagc atcaaacaca tctttttcgc gaacatcgat tacttgtgct tttctcattc
    10981 cttctcgaaa ttcttcttct gtcaaagtcg ttgctgaacg tttggccatt actcgtagat
    11041 agacttccca aaggatgatt gcaagcacaa tcaatagcaa aatgatatta attacccata
    11101 aaaccatgta atgcctccgt ttttttaagc aaatttcaag gctaatgagg cagcaccaat
    11161 gacaccagct tcattaccaa gctctgctaa tttgattttt gttgattctc ttacttgcgg
    11221 gaaagtaaat tctttaaaat atttctctac acggctacgt aaaaattctc ctgctgccga
    11281 tactcctcca cctaacacga tacttgacgg atttaatgta ttccccaagt ttccgcaagc
    11341 taaacctaag tagaagcata cgcgatcaac aaccatcaat gcaaacggat cgtcttcttg
    11401 agccatttca aagatatctt tacttgttac ttcttctccg ttgtctagca ttgctttcag
    11461 ttttgaatcc cctgcgtatt cttctgccaa gaaacgacct aggcgtacca cacctgtagc
    11521 gctagataca gtttctaaac atccgcgttt accgcaagta cattcgaacc cttcaggatc
    11581 cacagtgata tgtccgattt ctcctccagc tccgctcacg ccgtgaatca aatttccgcc
    11641 agcaacgata cctccgccaa cacctgttcc tagagtaacg aatacaacgt ctggatcgtt
    11701 ttctccagca cctttccatc gttcacctaa agcagcaaca ttcgcgtcat tatcgatagc
    11761 gaatttgatt cctgttcctg cctcgatcag ttttttcaca aattgaactt ctgtccagtt
    11821 taagttatac gcaccaataa ccgttccagc ttcgctgtcc acgcttcctg gggtacccat
    11881 gccgattccg ataaagtctt cggctttcat attatataaa ttcaaacgat gattgattga
    11941 ttcaatgatt tcaggaacga tatgcttacc atcatccaaa atattggtat cgatgctcca
    12001 tttttgctga atctctcctt ctggtgttaa aattgcaaat tttgctgttg ttccacctaa
    12061 atcaatccca ataatcttct tgtccattac aaatttcctt tctttcggct ttcttcaatt
    12121 cggtgctctc tttttaatat gcgccacgct gtcatgtatg tatcatgatc aatcaaacgg
    12181 ctatcataca gattacgcaa ttcgatagtc attaattcaa tgtcatagat gcgtgcaccc
    12241 acatagataa agatgccaaa ttttttgaac agctgctgta catcatataa gctctccatc
    12301 tttaagcact tccttcatat tatacaagta aaccatattt ttttaaaccc agtaaaatac
    12361 atattacaaa aagaaataca aaaactagtg tagaaagaat cctttggtgt atcgaatagc
    12421 ttgtctgttg tttcggtaga cccaaaacat tccctaacac taatccgcca attagcccac
    12481 cgacatgtcc ccaaacatct acagaggtat ccagaagacc aaaaacaaat gtcaacacaa
    12541 taaataaaag aaaatgtctg actagctgct ggatcgctgg gttatgttta aaatgaaacc
    12601 ctaagataaa caacgcaccg aacaaaccga atatcgaagt acttgcacca ccagacagaa
    12661 cattcgcttc gttaaaagca aagcttgctg tatttcccat tatcccgctg aacatataaa
    12721 tcaagaaaaa acgccaatgg ccatagattg cctcgatttg ctgccccata aaatacagta
    12781 tcagtgagtt cattacgaaa tgcatcagtc caaaatggat gaagattggt gtcactaaac
    12841 gccaccattc attgaagtga acaagataag gaccatacat tccggcataa taagggatct
    12901 caagtcgcgg aaataattcc atcatgagga agaccgctgt ctgtatccct aaaaaagcat
    12961 aggtccataa cggtcgtctt aaccatgcac gcaattttat ttgctgttga taattcatga
    13021 aaacctccca tttcatttta gtcaataaac aattgttgga tcttctgatc aaagctctcc
    13081 ggttgccatt gttcattcaa ttgttcacga aaaacaaggc tacatgtttt tttatgaaaa
    13141 ttatttaaat accggtcgta ataaccaccg ccaaaaccga ttcgataacc ggctctattg
    13201 tagataatac ctggtacaat gaggagatcg atcatctccg gcaaatagag cttctctgat
    13261 tgtggctcaa gaacaccaaa actcgaacgt acaagaacgg actgttcatc catttcataa
    13321 aacgccatct gacgtttcgg caaagtttta ggtacaacta ttttcttttg ttctttttct
    13381 gctcgttgga taaccggctg tgtatccagt tcaaattcat tcgataaagt cgtccctatc
    13441 acttttgctg acttccagta ctcgctggcg aatagctgtt tatagattcg ctgttctttt
    13501 tcctttttct tgccactttt ggccagattt ttcagctttt cgattccttt ccggcgtaat
    13561 cttactttca attggtccaa aaactcaccc ttccttctct cataaaatgg tataggaaaa
    13621 aaagaaaaat agcaatcgaa ctttcaaata aatgcataaa tagaataaaa agaagaccta
    13681 gaaatgattg gccttccttt tttaatgaat ttcaactgtt cagcgcgtgt ttttgtctga
    13741 taaaaagtgc atacagaaaa tatggcgcac caaaaagggc aatcaaaatt cctgtaggta
    13801 cttccccact tggtagaatc actctagcaa ccatatctgc actaagcaga aaaatggaac
    13861 cgaacaaacc gctcagaaca aaggtccagt tattttcttt acgtacgact agcttcgcag
    13921 catgaggagc tagtagtcct acaaaagata aactacctgt caccgctaca ctaccgcttg
    13981 ccaaagcaac agctaacaac aaaaggattt tttgactttt agccacgtta aggcctaatc
    14041 cttgcgcgcg ttcttgacca aatgatagca atacaatcct ctgtgcataa agtcctccta
    14101 gcggaattaa gataaccgcc catggaagca atgcccaaac ataaggccag ctcgcacccc
    14161 agattgatcc cgctaaccat gcattcacaa atccataatt ttccttagat aattggatcg
    14221 tgcatagcaa tgttaatgca gaaaggccgg cattaacggc aataccggac aataagattc
    14281 gattcatacc caaaaagcca tttcgatcat aggccgcaag atagacacag caagcagcta
    14341 aaacaccacc tagaaaggct acaactagca atagaaacgg cagcgaatag tgcgaaaaga
    14401 aacctaaata aagcatcact aaaaatcctg ctccggcatt aatgcccaat atactagaat
    14461 ctgccaattc atttcttgtt actccttgca agatataacc agaaacgcca agacaagctc
    14521 ctgctaataa actgatcagt aatcgaggaa ctcgaaattc tctaacaatc agcgccaaag
    14581 aagatgattc tttccctatg aacgtggcaa aaagctgatc caggtaaata aaaggcgtac
    14641 caatcattaa actacctata gacaggatga caagtacaaa aagcaagaaa tagaacgtcc
    14701 aatatatttt tttcaccgtt gttccctcct tacttgtatc agtaaaaacg gaacgccaat
    14761 caatgcaatg actaatccaa aaggtgtttc aaaaggggga ttgatcatcc tcgctgtaaa
    14821 atccgccaac ataaccaatc caccgccaac taaaaaagag acaggtagaa cataacgata
    14881 gtccgttcca accattccgc gaacaactgg cggaaccaac agcccaataa atgaaacagg
    14941 tccgactaac gaaacggcaa tggctgctaa cactgcaact agacagaaaa caatcaaccg
    15001 cgttcgttta ggatgacttc caagagctaa tgcatgtgtt tcactcaaat gaatcaaaga
    15061 tattgctttt cctaatgaaa aagaaaccaa aacagcaagt atatataaag gaaacaatac
    15121 tgctaattgt ttccatgtga tattggcagc tccgcctaca aaccaaaaag caagatcctg
    15181 ctgcagatta aagaattggg taagtgcttg gctgatagca gtaaaaaaag aactaatagc
    15241 tacgccagct aaaatcagct gtaaaggatt catgcccatt ttcgaaaact gcaaaaaacc
    15301 aaatacgagc aaagttacag caaaagaacc tgccaatgac attagaaaca cagaaaaatc
    15361 agaagcttta ggccaaaaag caaaactaag ggccattgcc aacgaagcac ccgcgttgat
    15421 tcctagtaag cctgaatctg ctaaaggatt tcttgtcacc ccttgcatca atgcaccgct
    15481 caatgcaaaa caactcccta caagaaatgc tcccgccaca cgaggcaaac gaatatttcg
    15541 tatgatttgg tgactttgtt ttgatctatc aaaatcaaac aacgcctgcc aaaaatctgc
    15601 tttttccaca gtcgctgcgc catttgaaag agcaacaaaa aaaataatca aaagaacaaa
    15661 acagaccaaa agcaggagtc tatttttctt catagtcctc ataattgatc ctcctgctct
    15721 tttggcaaca aatcataggt tagaaaaact ggtttatttg ttcttggaag cgtgacaatc
    15781 gttgcatcaa tctcaaatat ttttttcata cactcttttg tgatcacttc ttcaggtgta
    15841 ccttctgtga tcaacttacc gtttttcatt gctaataaat aatctgaaaa acgtgaagca
    15901 tgattaatgt catgaatcga catcaagatc gttgtttgtt tcatttgatt gatttcagtc
    15961 aataactgta ggatctctaa ttggtgtgcc ggatctaaaa atgttgtcgg ttcatccaag
    16021 atcaagatat ctgtttcctg agcaagcgcc atagcgatcc agacacgctg ggtttgtcca
    16081 ccagacagcg ttgcgatatc ccgcatacga aatccagcta gacctgtagc atccaacgcc
    16141 cactcaattg cttcatgatc ttgcgtgtcc atgccactga acagtttttg ataagggtat
    16201 cgtccgtaag atactacttc ttctactgtc atacctgtag ggtgttcact agtttgtgct
    16261 aataatgcaa ttttttttgc aagttctttt gaactgtatg tagagatatc agagttattg
    16321 acaatgatct ttcctgattt cacaggtagt atacgggtaa aacttttcaa taaagtagat
    16381 tttccacttc catttggccc aatcaaacta gtgattttcc cttcaggaat cgtaacagac
    16441 atgttttcaa tgattttttt gttttcgtag ccggctgcaa tgtcagctgc tcttaatctc
    16501 gtcatttcac ccatcctttc gctgaaaatc attctcaatt atgacgctgt ttcaacattt
    16561 cgtcaagcgc tactttcata tctatagcca aatgaagcgg ttgacagcgt tcgaaatatc
    16621 aggaataata accaatgaga aagattctca attatgttag gaggaaagaa atgaaattaa
    16681 aaacaaaagt attcacatta tctagcatat tgctgctggc tggagtactg agcgcttgct
    16741 ccccctcttc agataaagaa acagctacaa ctgccaattc gacgacttca tccaatagta
    16801 ctcatacagt cacagataca cttggacata aagtagagat tccaaacgcg cccaaacgga
    16861 tcatcggcag ttacttagaa gactatctga ttgcactagg ggaaaaacct atcgcccagt
    16921 ggactgttgg cagtggttcc attcaagatt atctgcaaga tgatttaaag gatgtcccaa
    16981 cgatttctta tgaccttcct tatgagaaag tgttaagctt tgagcctgat ttgctgttga
    17041 tttcttcttc cgctactgtc gaaggcggga aatacgaaca atatagcaaa atcgcgccaa
    17101 cttatgttgt aaaaaacgga acagatgtca catgggaaga acaactaaaa gatgtcggca
    17161 aagctttggc aaaagaaaaa gaggcagaaa aaatcatttc cgattatcaa aaatccatca
    17221 agaaaactcg agaagaacta gcagataaaa tcgagaacaa gacagctgca gtgttgtggg
    17281 tgaccaacaa ctcagccttt atggtcagca aaaatcggtc aagtggaaga atcgtatacg
    17341 acgatctgga atttggtgtt cctgatctag tggaagaggt ctccaaaaaa gccacttctg
    17401 actggtctgc tgtttcctct gaaaagctag ctgaattaga tgctgactac atcatccttg
    17461 tgaacagtga caaagatgct gccatgttca atgaaccaag ctggaagaat ctaaaagcag
    17521 tgacagacaa tcatgtactg caattcggac ctgaatctag ttggctatat aacggaccta
    17581 ttgccagtca ggaaatggtc aatgatatca aagaaaaact aaattgattt taactaaaaa
    17641 aagcatcttc acgattagtc gtgagatgct ttttttctta ttaacaaact agacgcatag
    17701 attttattct tcttttgaag caaccctatt ttttactctt tcccattttt gaaaacgatg
    17761 ggcatataga ttttcttgat ccgttatccc ctcatactcc tctataaggg cgaagtctga
    17821 aaaatcaata ttcccaatat acgtatcgcc ttcaaacgtg ctatcgatca atgttcgcca
    17881 gatccgacca gtgtaaggaa gaagttcttg aaaaatccga ctaccacccg aaacatagat
    17941 atcttctgag atcgtttgac tatacgacaa aagttcatcg atcgaatgca ttacttccgc
    18001 attttctttc gtgcgaaaat ttggttgcgt tgtcaacacg atgatatggc gataaggaag
    18061 tgaaagatcc cccattcctt cataggtctt tcttcccatc actaaaattc gatccattgt
    18121 atgttcgcga aagaaacgca tatcatttgg caatcgccat ggtaacaaac catttttgcc
    18181 gatcagacca tttttatctt gtgcccacat ggaaataaac atattatccg ctccttatga
    18241 ttttagatgt tttgagataa aaaaagtctc aattctttga ccagcaagga attgagaact
    18301 ttaaaaacat tattttgttt cacggtgcaa agtaactttg cgttcgcgtg ggcaatattt
    18361 ttgtttttcc aaacgatcag gattgttacg tttgtttttg cttgttagat agttacgttc
    18421 tttacaagaa gtacactcta aagtgatgtt tacgcgcatg tttttccctc ccatttttac
    18481 aagaatcctc taagatgact ctcagacttg attatcttac catgattttt tctagattgc
    18541 tagtgttttt cacgattttg ttaagtaaaa ctacttgacg ataaatacaa cggttttact
    18601 tctcaaacaa aatctttttc tttttgtata aataccctta cacttgttta tatagattat
    18661 gttttttaat aaagggggat actaatatgc catggaacat gaaggacttt cctgcatcaa
    18721 tgaaaaactt agataaattg acaagaaaaa aagcaatcga catcgcaaat gcattattag
    18781 atgaagggta ccctgataac cgtgcgatcc caattgccat tgatcaagca aaaaaatggt
    18841 atgaagatgc tagtgaatca gaaaaacgaa ctttcgaaaa agaaaaaaat ccaagcaaga
    18901 cggatgaaca cgatacgaat cctcgtgctg gaaaactgct tgattcagat gtgatcgtag
    18961 aatatgagga agaacaatgg atcgtgaaat ctaaaggggc aaaaaaggca agcaaccatt
    19021 ttgatacaaa aaaagaagca atcgaaaaag gaaagcaagt agcacaaaac aaagaatcaa
    19081 ctttgttcat ttataaaaaa gatgggacaa aagaaaaaga aatcgattat tgaacatatg
    19141 taaaggggtt gtgacgaaaa tcgtgtcaca acccccttat gttcgaaaca atggtgttcc
    19201 tctgccaact ccgaaccata agtcataata tctataaaac gggaagagct gttttttgaa
    19261 attgtttctt atcactcagt gttaaacggt aggagaaaac ctctttttta ttgatatttg
    19321 attttcatca aacagtcaca tagtcgattg cttctgcttg cggaatcgtt tcatctacaa
    19381 agtagacttt ataccatgtc aagaaactga agattgtcca gatttttctt tgctcttctt
    19441 tacgtccttc gtgatggtca tctagcaact tcatgatttt ttcttgatca aaaaattctt
    19501 ttgcaaaatc tgcttcgaat aatgctttga cttgttcgta gccgtgttct tctttcaacc
    19561 aagctttgat tggcacaggg aatcctaatt tcacacgatt cgaccattct tccggaagat
    19621 gtttgttcgc agcttgtctg aaaatatctt ttgtgttgtt ttgattcaat aaaaacttac
    19681 ttggtatttg ttgagccaat ttcatgactt cgatatccaa taaaggtact cggacttcca
    19741 aagaacttgc catagaaagt ttatctgcct ttaacaagat atcttttggc atccattgat
    19801 gaagatcgac atattgcatc ttattgactt catctttgat tccttctgtt ttctcgtaat
    19861 gaacagtcat gatttcatcc acagatggtg ctgtttgata ttttggtgtt aacacttctg
    19921 atgcttctga ttcttcgaac actcgagcat gaccgataaa atattctttt gcaggagcaa
    19981 gtgattcata taaatggatc cgtccatgga agtttttcat ttttccgatt ttacgtccta
    20041 aattatagcg tgttcctttt ggtaattttt tcaatccttg agcaatcaca cggatgaatt
    20101 ttgaattcgt atggaatcca tacgtttggt atcccgcaaa cagttcatcc gccccttctc
    20161 cagattggac tacccgcaca ctttgtgaag caagatttgc taaaaagtac aaaggtacgc
    20221 aagaaggatt tgaatcaggt tcatctaagt gatactggat caaagggaat gctttgaacg
    20281 acatgtctcc gtcgatcact gctgctgtat tatctaagtc caatagttcg gttagttttc
    20341 tggcttcaat cgcttcatta taggttttat catcaaaccc gattgagaaa gaatgatctg
    20401 gtttcaatac agaggttaca taactggaat caaccccgct ggataagaaa gaaccaactt
    20461 ctacatcact gaccgtatgt gcttcaatag aagcttgaac ggtttcgtcg attttttcga
    20521 tccattcttg tcgggaatgc gtttcttttg tttcaaaatc tgcatcccaa tattgctgaa
    20581 tgtctatttt tccatcttta tacgtataat aatggccttc aggcaaacga tagacatctt
    20641 tgaagaatgt ttcgccgttt aatggagaat attggaaagt catataaggt ttcaacgctt
    20701 ctttgttcaa ttctttgtta aaatcaggat gaggcagaaa acttttgatt tctgaaccaa
    20761 acataaatgt tccgttcatt tctgcataat aatatggttt gatcccaaag tggtctcttg
    20821 caccgaataa ctcattcttt tcattatccc aaatagcaaa agcaaacatt ccgcggatct
    20881 tttgcaataa ttctgttccc cattcttcgt aaccatgcaa aaggacttct gtatctgcat
    20941 gtgtttgaaa aacgtggcca gctgcaatca attcttctct caaaggctgg taattataga
    21001 tttcgccatt aaaaataatg atctttgtcc gatcttcatt gtaaataggc tgagtaccgc
    21061 cctctaagtc aataatactt aatcttctga atcctaaagc aacatgttta tcgatatatt
    21121 ctcctgaact attaggtcca cggtgaacga ttcgatccat cattgtatta ataattgttt
    21181 ttttgttgtc cttgtcgtta acaaaaccta caataccgca catttaactt tcacatccta
    21241 atataaaatt actgtttatt tcattcatat gcatcttatc atagaataaa tagcggacga
    21301 atattttctc tcttacaatt tcgtcacgtg aaaacaaaag atgaaagttt ctgtttttac
    21361 agaaaataaa gtcccaatat ctaatttacc atgttttctt ttttgaggct accttattct
    21421 cttcgtgtta aaaaaatatt agtggatgct atttccaaaa aaaccagcaa aaaaagacac
    21481 gacctaaaga tcgtgt
//
LOCUS       DAZUKZ010000039        21193 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000039 DAZUKZ010000000
VERSION     DAZUKZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.039"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..1328)
                     /locus_tag="ACG8EF_001415"
     CDS             complement(66..1328)
                     /locus_tag="ACG8EF_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4946729.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1321..2220)
                     /locus_tag="ACG8EF_001416"
     CDS             complement(1321..2220)
                     /locus_tag="ACG8EF_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4946730.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2365..4143)
                     /gene="uvrC"
                     /locus_tag="ACG8EF_001417"
     CDS             complement(2365..4143)
                     /gene="uvrC"
                     /locus_tag="ACG8EF_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFS4946731.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4325..4639)
                     /gene="trxA"
                     /locus_tag="ACG8EF_001418"
     CDS             complement(4325..4639)
                     /gene="trxA"
                     /locus_tag="ACG8EF_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFS4946732.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4726..7086)
                     /locus_tag="ACG8EF_001419"
     CDS             complement(4726..7086)
                     /locus_tag="ACG8EF_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFS4946733.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7201..7748)
                     /locus_tag="ACG8EF_001420"
                     /pseudo
     CDS             complement(7201..7748)
                     /locus_tag="ACG8EF_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            complement(7806..8243)
                     /gene="zapA"
                     /locus_tag="ACG8EF_001421"
     CDS             complement(7806..8243)
                     /gene="zapA"
                     /locus_tag="ACG8EF_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFS4946734.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8445..9362
                     /gene="rnhC"
                     /locus_tag="ACG8EF_001422"
     CDS             8445..9362
                     /gene="rnhC"
                     /locus_tag="ACG8EF_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFS4946735.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9618..10529)
                     /locus_tag="ACG8EF_001423"
     CDS             complement(9618..10529)
                     /locus_tag="ACG8EF_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS4946736.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(10932..11510)
                     /locus_tag="ACG8EF_001424"
     CDS             complement(10932..11510)
                     /locus_tag="ACG8EF_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4946737.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(11530..12198)
                     /locus_tag="ACG8EF_001425"
     CDS             complement(11530..12198)
                     /locus_tag="ACG8EF_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4946738.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(12213..13283)
                     /locus_tag="ACG8EF_001426"
     CDS             complement(12213..13283)
                     /locus_tag="ACG8EF_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4946739.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(13454..14818)
                     /locus_tag="ACG8EF_001427"
     CDS             complement(13454..14818)
                     /locus_tag="ACG8EF_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4946740.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(14815..16374)
                     /locus_tag="ACG8EF_001428"
     CDS             complement(14815..16374)
                     /locus_tag="ACG8EF_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4946741.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(16430..18910)
                     /locus_tag="ACG8EF_001429"
     CDS             complement(16430..18910)
                     /locus_tag="ACG8EF_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946742.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(18894..19229)
                     /locus_tag="ACG8EF_001430"
     CDS             complement(18894..19229)
                     /locus_tag="ACG8EF_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946743.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(19243..20277)
                     /locus_tag="ACG8EF_001431"
     CDS             complement(19243..20277)
                     /locus_tag="ACG8EF_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS4946744.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(20367..21047)
                     /locus_tag="ACG8EF_001432"
     CDS             complement(20367..21047)
                     /locus_tag="ACG8EF_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4946745.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
BASE COUNT         6047 a         4435 c         3620 g         7091 t
ORIGIN      
        1 aggtctatac aaaaaagaag ctgggaacaa taaatgtcca gcttccttct atatttttct
       61 atattttatg ctttttttcg ttcatttctc ataattgaaa tcgattgttt caatgaactc
      121 cataatccgc cttcgctata cacgagtaca ttcgatttgt acatgtttgc agacaagatc
      181 atcaataaga atgttacggc tacaagtacc aacaaggaaa tcaatgctcc acccataccg
      241 actgtctcat ttgctaagcg aaccggcata atatatgaag ataagaatgg aatatacgaa
      301 gtgatccgaa taacgatatt gtttggatct gaagctccta gtatcaatcc aagcatatat
      361 ccgcctagag atagataagt cactggcaaa attgcttttg ctgtatcttc tgctttgttg
      421 accaaagagc cacataaagc tgctaaaaca gaaaaaatca aaatgccaaa gatcatgaat
      481 agaagagaat accacaagaa tggtccaatc aagttatcca atgagatacc gtctaatata
      541 tttttgacca tttccatatc tttgaattga ctgtatccta taccaaaaat aataccgtat
      601 aatgccattt gcgttaatgc aacaagtaaa acacctgtta gttttccgta aaaatgcttt
      661 tgtgcagtcg tactggacag gatcacttcc atgatccgcg tgcctttttc agaagcgatc
      721 tcttgtgcaa tgatctgcgc atacgtcaaa ataataatga acaagacaat cgttgctaca
      781 taacttacga catattggac tgtcgagtta tcttctccaa ctgtcatttt tccttttgaa
      841 tcaaaattga ctttttgtcg agtcaaactt gcttgttggc tcaagctagc tacttcttct
      901 ggtgaaatgc ctaattgact tgctcgaagc atcgattgca agccagtcag ttgttgttgt
      961 atcagtagtt gtgtactttg tcctaatgaa ttttcgctat aaagcatccc atttatctta
     1021 tcattctcag ttgagactac taaatacgca tcgatttttt cttccgaaag ctctttttgt
     1081 gctttttctt cagaatcaac tgctttgaac tcaaaatcat ctgttttgac ttggctcatt
     1141 tgctgactga tttgctcatt atcagccaat acgccgattt tattgacatc actattttgt
     1201 tgagcaaagt ttcctgctac ataaatgata cccagcatga taaaagggac taatatcatg
     1261 atcaagaaag aaatcgactt aacatttttt aaatagacat ctttggtaat aatccaaaac
     1321 ttacgcatcg atttcacctg ctttcaattt gaaaatctct tccaaagttg gcggttgctg
     1381 gttaaacata ggaatgtagc cgaactgagt cgcacgagta aagatttctt tcccagcttc
     1441 aggatcttct aaggtaatct ctaacgtatt gtcttcatga aggatcactt ttttcacgcc
     1501 ttcgatttct tctacttcct gttggcttaa accagattcc aagaataact tcgtacggcc
     1561 aaatgattca cgaatctcat gtacttttcc attcaaaacc atttttccgt ttcgcaacat
     1621 gatcaaatga tcgcagattt tttctacatt atccatattg tggctagaaa aaatgacaca
     1681 tgaaccatgt tttttcaatt ccaatattcc atcttttagc aattcagcat ttaccggatc
     1741 taacccgctg aatggctcat ccaaaatgat caatttaggt tcatggatta acgtggcgat
     1801 cagctggact ttttgctgat ttcctttaga caaagatttt actttatccg tctttttccc
     1861 ttttacctga aatttttcca tccaaaaatc gatttttggt tcaatctctt ttcttgattt
     1921 ccctctaagt tctgcaaaat aaagcagttg gtcttgaatc gttactttcg gataaagtcc
     1981 ccgttcctcc ggaagataac cgatgacatc ataatctttc tccccaagat gatggccatt
     2041 ccataacact tctccttggt cttgtgtcaa aaaatccaag atcaaacgaa aagtcgttgt
     2101 cttcccagcc ccgttttggc caattagccc catgattttt ccatcgggga tttggaaaga
     2161 gacatgatcg acagctgtgt agttcccaaa agttttaaca agatccttga cttctaacat
     2221 ttcttcactc cttcttcaag aaatcacata tatccttaga caccagacgt aagcgattta
     2281 ttatttacca acaaaacgta aacgctcttt ttgttgtaca taaaatgata ggacacttta
     2341 ctttctttgt aaagtgccct ttcctcattc ctgatgtaat ttatcaaata cgttttgtgc
     2401 gacatttttc ggcagtccga tttcttgtaa ttcttctgtc gtggctgctg tgatattttt
     2461 caacgatttg aactctttaa gcagcatttt ttttcgtttt ggtcctaacc tttcaatttc
     2521 atctaatcgg gaagcaaaac tgtttttact gcgtagctgc cgatggaaag tgattgcgaa
     2581 tcgatggact tcatcttgga ttcgctggag taagaaaaac tctggtgaat ttcgctcaag
     2641 cggtactaca gccaaatcta taccgaatag caactcattt gttttatgtt tatcgttttt
     2701 tgccatgcca gcaataggaa tatcgattcc taattgattt tctaagacat cttttgccac
     2761 atctacttgt ccttttcctc catcgatcaa gatcaaatca ggtaaaggca acccttcgcg
     2821 aatcactcgt gaatatctgc gatagatgac ttctctcatt gatgcataat catccggtcc
     2881 gttgactgtt tttattttat atttccgata ttcttttctt gccggttttc catcaatgaa
     2941 tacgaccatc gcagccacag gattcgtccc catgatatta gagttatcaa aagactcgat
     3001 acgtattggg gtcgggatat tcatcgcgtt tcctaaacgt tcaactgctc cgatcgtccg
     3061 ttcctgtttt ctggcaatca gatcaaaccg ttcattcaaa gcaactgagg cattcttatt
     3121 cgccaattca actaattttt tcttttctcc tcgttttggt tgcagcactt tcgtagctaa
     3181 caaagcttct acactgggct tgtcgatcgt atcaggaatc aagacttctt taggaatgaa
     3241 atgctcattt tcctgataaa actgaccgag gtaagtcaag aaatcttctt ctgcttcgtt
     3301 atagaaagga aacattgaaa catctcgttc aattagcttt ccttgtctta cgaaaaacac
     3361 ttggacacac atccatcctt tgtcaatcgc ataaccaaat acatctcgat ccagtaaatc
     3421 agtatttgtc attttttggc gagtcatgat tgtctcaatg gcacggatct ggtctcggta
     3481 ttctgcagct ttttcaaact ccatgttttc agcagctttt tccattttct gatggatctc
     3541 tgactcgatt ttttcgtgtc ctccgtttaa aaactttttc acttcatcaa cgattttcgt
     3601 atagactttc ggatctacgt cgaaataata aggacacaga cattgtccca aatgatagta
     3661 aagacaaggc tccttcgtct gactaggttt gcattttctc agaggaaata atcgatcgag
     3721 gatcttcttt gtctcattcg ccgcaccgac atcgggatat ggtccaaaat acagtgcttt
     3781 gtcttttagt actttacgag taatcaccaa acggggatat ttttcattcg ttatcttaag
     3841 gaaaggatag gttttatcat cctttaacat aatgttgtat ttaggatcat ttttctttat
     3901 cagattgatc tctaagagca atgcttcgat attcgattct gtaacgatgt attcaaagtc
     3961 ttcgatttca ctaacaagcc gttccgtttt cgtattatgg ctgccagtaa aatatgagcg
     4021 gacacgattt tttaatattt tggctttgcc gacataaatg atcgttccat ttttatcttt
     4081 catcaagtaa cagccgggct gatcaggaag taaagctaat ttgtttttga ttcgttcgtt
     4141 catctggtac tccccttcat attcaattaa agttattata gcatgtctgt caaaaaaaca
     4201 atccagcctg acaacgtaat tatactcaaa aaatattaat ttgaataacc ttaactcaac
     4261 cgcaaaaaaa ccgaggacgg aactggattc agtcccaccc tcagcttatt tttgctgtaa
     4321 gtttttataa atattgatcg atcatttgac gtagttgttc ttttgaatga acaccaacag
     4381 ctttttctac tacttgacca tcttttttca acattaatgt tggaatgctc atgataccga
     4441 attgttgagg agtttctgga ttttcatcca catccatttt agcaattctg aattcttctt
     4501 catcatattc ttgttccaac tgatccaaaa tcggtgcttg catacgacat ggaccacacc
     4561 aagttgccca aaaatcaatc aatacaagtc ctttatctgt ttcttcatta aatgtcttat
     4621 ctgtaattac ttgtgacata tcaaagacct ccatttttaa aatctatgat tagtatagca
     4681 tttctatact tggatagcga accatctgct tacctttagt aagctttact taaatttaac
     4741 aattgtcgca ccatttccgc cttggtttgc cggtgcaaac tcaaagcttt tgacgctccg
     4801 atggttttta agataatcag taattccctt gcgaagtgcg cctgttcctt ttccgtggac
     4861 aatagtcact tgaggataac cagctagaat ggcagaatcc aaatattgat ctacctcact
     4921 tagcgcttct tcgtatcgtt ttcctcgaag gtccaattga ttcggcacgt gactactaga
     4981 ttcagatcgt acagcatgga taactcgttg tgtcggctct ttttgaggag caacaggcgt
     5041 cagctcgtct tcggacacat tcatttttaa tataccgatt tctacttgcc actgattccc
     5101 gtttttacgt aaaagcgtcc ctctttggcc gtaagtcgta accaacactt cgtctcccgg
     5161 tttcaacgtt ttttgttctt tcgccttctt caatacttta tttttcttca aagtttcttc
     5221 ttgatgtaga tttgctaatt gggtctttgc atcgatcaac tgatgctctt tcacatttcc
     5281 ttgcccaatc tgctgttgca ttttacgaat atcagcaatg attttttctg ctttttcttc
     5341 cgcctctgac acgacttcgt ttgctttttt ctttgctttt tccatttctt tttcacgttc
     5401 ttcaaagaaa tagctgtaag cttctttcaa atcatcgtga agttcttgtg cctcagaaac
     5461 aaaatgacgc atttccagat attcggtctc tgccattttc cggcgattct ctaaatctgt
     5521 gatcatttcg tttaaatctt ggctctcatc attcatcaac tgcttcgctt catcaataac
     5581 ttctgtatca agacctaatc ggctagagat ttcaaacgca ttgcttcgtc caggaacgcc
     5641 aatcagcaaa cgatacgttg gacttagtgt atccacatcg aactccatgc tggcattaat
     5701 cgtatttgcc cgattgtatc cataaacttt caattcagga taatgcgttg ttgccatcac
     5761 ataagcagac tttttaccaa gatcatctaa gattgcgatt gctagagcag caccttcttg
     5821 cggatctgtt cctgcaccga gctcgtcaaa taataccaga cttttttcat tgacttttga
     5881 taaaatatct acagtgttcg tcatatgaga agaaaaggtc gatagacttt gctcaatcga
     5941 ctgttcgtct cctatatccg caaatacttc ctcaaaaata ccgatttgac tttcttcacc
     6001 tgcaggaatc ggtaatcctg cttgccccat cagttgtaac agacctaaag tcttcaacgt
     6061 gatcgttttc ccacctgtgt taggtcctgt aatgacgatg gcttgatagt cttttccgat
     6121 tgtgatgtca ttagaaacta ctttttcctg atcgatcagt ggatgtcttg cttgtttcaa
     6181 gacgacatga ttgtcttcac tgattcccgg cacgatggct tttacttctt taccaaatcg
     6241 tgcttttgca ttcatcagat ccattttacc aatcacataa gcattgtgga tgatttcttg
     6301 acgatgggga acaagttcag cagaaagttc acttaaaata cgttgaatct catttcgttc
     6361 agcgatttgg tattgacgca aacgattatt caaatccacc acttgttttg gttcaataaa
     6421 taaagtctgt cctgaagcac tttgatcgtg gactacaccg ccaaatacac cgcggtattc
     6481 ttgtttgact gggataacgt agcggtcatt tcgcatggtg atgatcgcat cacttaaata
     6541 ctttgcattt ttcccacgga cgatcccatc taactgttca cgaacagctt gttcacttcg
     6601 ccgaatgttc tgtcttatgc tctttaactc aggagaagcg tcatcagtca cgcgtccgtc
     6661 ttcatcgatt gcttccttta aacggcggct caaaacaggg atcgtgacta actgatctgc
     6721 ccacatatac aaacgtgcaa attcgatttc actgtcagaa agatcatcaa tgaaacgatt
     6781 caattcgctt gtcgtagaca atactctgga aacttgtgca agctccactc cattcaagtc
     6841 tgctccgatc tcgatccgtt tcatatgggg acggatattt tcaagtttag gtatagggat
     6901 cccgcctcgc agacgttgca ctttcaatcc atcctcagtt tcttgaagcc agtttgtgat
     6961 cgtttgtttc tcactcactg gtaccaattc tgccagttct tcttttcctt gtgcagtcac
     7021 tacaaattgt ctgaccatct gtttgacttt ttcaaattcc aatgtttcaa ggattcggtt
     7081 attcatttta tcaccttatc tttcactatg aaaaaagagc tgtgccggac atacgctacg
     7141 cagccttctt aaataaacta ctctgtacaa aaaacgaaat attttccagc actattttcc
     7201 ttaattcaaa atattcgtta cccaaagctc atacatcttg ttggtaagaa atggcgttct
     7261 ctcaacgata aaacgagcga aactatgctc gccgaattga ttttggacca cactaatgga
     7321 acaaaggcaa gtaactgcaa aatcaaaaat atagtaacgt aagccatgat tagtgacagg
     7381 atgcctcctg caatccagtc tacctgtctc aagatcggca catacgtcaa tccatggaca
     7441 aaaaccccca caaatcgggt aagcagccag ccaattagca aaataatcag aaaagcagtt
     7501 cctgcataaa aagcttcatc taggtgaaaa gcaaatacct gatcaaaaaa tgctaacttt
     7561 gatgttgggg tgacagctgg gtatggaatg tataattcca aatgacttgc taatggtttg
     7621 taaaaatgct gggcagcaat aaatgacaat acatatccaa tcgcgaatat gatttgcaaa
     7681 gcaaacccgc gacgtgctcc agaaaaaaac gcaattagca ataaaaataa aatcagcaaa
     7741 ctcagcatgc tacgttcctt tctcttctaa cttttttaaa tttttcttat tttgataaaa
     7801 ggatcttaat tagaagattt ttgtttaatt tgtccttttc gtttctcgtt taaaatctgt
     7861 tgtgcttcca catgattttt aatggtatga tctgcttgac cattttcttt caatacttcc
     7921 cgagcttcgt tctcgattgc ttcaatgcgt ttcacacgat tttcaagctc tgtaaatttg
     7981 atcatttttt tctttaattc tgctgtttct tcttccagct ccaagattct ttcttgtttc
     8041 ttcaattgat ccgaaagcgc attgacagcc atcaagattg ctgcttgttc attatccatt
     8101 tgtggtgata attgtttcaa ttctgctaac tgttcattga tcagcttcgt tacgatatcc
     8161 atatgatgct tggtttcccg tccgataata gtatatgttt ggttggcaat cactgctttg
     8221 tagcgtgttt tttcgtgcgc catacgaatc atttcctcct atttgatgaa agttttattt
     8281 agtgtaccat gaagttggtt ctttcaccgt tctccacaaa tatttttcac atacttaact
     8341 attatatgat aaactttaaa aaaatgctat gtctcgcaat ctcttagaaa acgaaaagtc
     8401 gtgagttgcg gtatagtttt atgtataaaa acgaggagaa ttttatgagc aacgtcatta
     8461 tcaaagcaac aaacgaccaa cttagtcaaa tgaaagcata ctatcgctct tatttgttaa
     8521 ataaacagat tccttatact agctttgctg caaaaaaaga cgggacgacc attaccgctt
     8581 atacatccgg aaaagtgatg tttcaaggta cgaatgcagc aatagaagct gataaatggg
     8641 gggaggcaca ggataaacaa gccaagccag caaaaacagc ctcttctttg ccaaaaaata
     8701 tcagtcaatt atccgtttta ggtagtgatg aggtcggaaa tggcagctat tttggtccgc
     8761 taacagtatg tgctgcttat gtgaaacgtg atcagcttgc agaattacgt aaacttggtg
     8821 ttagagattc aaaagagtta aaagatagcc aaatcatcca gcttgctgac atcttaaaaa
     8881 aaaccatccc ctataaactg cttgtcttat ctccagaaaa atacaatgag atccaaccaa
     8941 aatacaatgc cgttcggatg aaagtcgctt tgcacaatca agcgatccat ttgctgctaa
     9001 aaaaaatcga gccagaaaaa ccagaagcga ttttgatcga tcagtttacg ccggaagcaa
     9061 attttaaaaa atatgcgcgt ttagaaaaaa atcaattaca agaaaaactg tacttcgtta
     9121 ctaaaggaga acaatatcat ttagctgttg ctgctgcttc tatcatcagt cgtgccagct
     9181 ttttggaaga gttacacaaa gcatcagctg aagctggaat cacccttcca tcaggagctg
     9241 gaacgaaatc tgaccaagta gcggcaaaac tattggagaa aggcggcatg cctatgcttg
     9301 aaaaatatgc caaacttcat tttgccaata cagaaaaagc tttgaagctg ctaaaaaaat
     9361 aatggaactg tgacaaaagt catgtcacag tcccttgttc cgaataaacg gtgttctcat
     9421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     9481 tgctccttgt gacgcggagt taaacggcag gctcacaacc tcttccttaa acggtgttca
     9541 aaagcttgca tctgcgataa taagcccggc caaacgaaaa atatgaggag ctatttttgt
     9601 ttggctgggc ttatttatta ctgaaaataa gtggtcaatg cttgtgtcaa acttgcagca
     9661 atctgctgct gataactgtc tgttactaat tcctttaaat cactatcatt attcatataa
     9721 cccatctcga ttaaaataga aggttgaaga ttttctcgaa gaacttgata gttcccagtg
     9781 gagattcctc ttgaatccag aggcagatca gtgaaggagt cagcaattgt ctgtgctaat
     9841 gtttcctctt gtccatagta ataatatgtc gtgattccag ttgcttcatt cgcatattcc
     9901 gtagagtctg cgtgtaaact gataaagaca tctgcctttt tttcttcact gtagtcgcat
     9961 atatctccaa gctgtactaa ttcatctgta gtatgggtca aataaaccgt tgctccagca
    10021 tcctctagcg ccttttttac ttttatggct gtacttaatg ttatatcttt ttcttctgtt
    10081 tgttcatcat tgcttaatgc cccagtatca tttccgccat gcccaggatc taatacaatc
    10141 actgcatcag atagctcttc cggttttgtt tgtatattct ttgtgacttt ggttccatct
    10201 tcgagggcta aatatccagt atagccatca tttgtcttga tctgataagc attttttgtt
    10261 tcagaaagaa actccgccag ctctccgccg tttatagttg caagtggggt agttagcgag
    10321 tcatctgtat atagtgtacg gtcatttttt ccgattttga ttaatgtctc ttgttctgta
    10381 tcatcaaatg attcctttgt ctttgtaacc gtactgttcg tactgctgac cgtttgtcca
    10441 actatctttt tttgaccaaa aacatctatt tgaaaacaga taataatgat aatgattgca
    10501 aatacaaaag ctccaattat ttttttcatt cttgactctc acctcgtaga tccagactaa
    10561 gggtcaaaac ttaaaatcag cttaagtacg caaaaaaggg gctgtaaaag atataaccgt
    10621 agaaaataac attactgcta aaatgatttt tttcattttt ggcatatcct gaaatctcct
    10681 tttgactatt cgtggtgatt catctaccca ataaatagtt tccttcattc tacaaacaat
    10741 taatttcttc gttattaaat taaaacactt cctggaacac cgacataata aataaaaata
    10801 taccctgata atcatgagag aataacaaat ttgtatttat gaaaaactac ctatataaaa
    10861 aaaacaagat ataacgatct tcgtcatatc ttgcttcttc acctaactat ttttctccct
    10921 atataacagc tttacgcttc tcctaataca ccattcaaat ataaatcgat caattgcgcc
    10981 acatttagcg gttcttccac aggtcttttc gaccgagtga tgtaaggtgc taacactaag
    11041 aaaaaatgcc ttgctgcgat ttcttttgaa atcccatttc ttagcacaga ttggtttgct
    11101 gaaaagattt cttcaaaagg ttcacgataa tttttcttcc atagaaagaa aagatgatct
    11161 cttgtttcct ttgaaagatt gctttcaaga tcgtgaagca tcagaaacaa atcgaccaga
    11221 tgtgtctcaa tcaaaaactg cgccattttt gtcaaggtta cttgaaatcc cgtttcgctt
    11281 gtttgaacga tttctctcaa agcttttcca acatctttta agtggttttc taaaactgcc
    11341 gtatatagtt tttctttatc accgaaataa tgatagagat tgggttgggt gatcccaact
    11401 ttttgtgcga tctctcttgt tgaagtgccg agatatccct tggacataaa caagtcagtc
    11461 gcttctttca aaattttttg ctgtgtatct ttgttgtatc gatggaccat ttttccacct
    11521 gctttttggt tattcttttt ctttcaatac accatcttcc atcataaata ttctatcgca
    11581 tttgtcaatc agccgtgtat cgtgagtaac catgatcgtt gctttatttt tgtcttttga
    11641 ttcttttgcc aagatatcga ctacttctcg tgccttttct gaatccaaac tagcagttgg
    11701 ttcatctgct agaataatgg acggatcatt gtacaatgca cgagcgattg cgaccctttg
    11761 ccgttctcca ccagatagtt cttctggata cttgttgatc aacttatcca cgccaagctg
    11821 ctcaaataat tcttttgctt ctttttgacg atgttcatgt ttgatcttat ctactaataa
    11881 taactgcttt tttactgtca gaaaaggaac cagattggaa gcttgcaaaa taaagccaat
    11941 ttctttaaaa cgtagtttaa cgcgttcact ttctttttga ttactgaatt cttgatcatt
    12001 gataaataca gagccactgc taggcgtctg caatccgcct gccaaagtca aaaaagtact
    12061 tttacctgaa ccagaaggac cgatgattgc aacaaattcc cctttatcga tactcatggt
    12121 tgtttctttc aatgctttaa ctacttggtc gccatcttgg aaactttttt ctacttcttt
    12181 aaattctatt gctttcatgc tcttcctcct tcttatccaa ttgccttcaa cggatcgatt
    12241 tttacgattg tccgaacaga aaatagtgcg cctaacacag ctactaccag catcaacaag
    12301 ctaatgacgc caaaaaacag ccaattgctt tggaatggaa ccgcatcagg tagaacaagg
    12361 gatgttccca ctgttcctaa tagaccaaga agaataccaa caaatgataa aatgaatgtt
    12421 tgtgccacta cagaacgcgc aataaatcct cctgtgatcc cttgtgcttt cattacacca
    12481 aagatattaa tcttttgcat cgtcagtaca taaatgaaaa tcccgatcac aatagccgca
    12541 ataacgatca aaaatccgat cataaatcca aaagtcaata cttgcgcatt atatccaggt
    12601 aattcattga tgaatttaga aattttgatc tgttctaaat cttcaggtaa attactgatt
    12661 ttacctctga caactattgc gttaatccgt gcattttcac ttgtatcttc tttttcgaag
    12721 cggatctctt ggtaagcatt gatggttgta tagagaactg gtgagacatt gaatttggca
    12781 tgatccgtaa agccagttag tttaaacgtc ttgtcactac cagataactt aactgtatct
    12841 cctacggcta gaccatattc ttctttcaaa ctaatatcgc ctactgcctc atcatcgtta
    12901 tcaaaagctt ttccttcaac aagattgggc atgatgaatt gatttttatc gataccgaag
    12961 aagctaacat tgatttttcc ttcttctgta ctgtctttac tagtcacgac tccaggtgtc
    13021 tgggcaagat acgccacttt cccaccttcg acttcttctg ctatcttctt agtaatcatg
    13081 gacatcccta gatttgaatt tgcatctttc gaaagcacaa tcgaatctgc ctcccacttg
    13141 tcgactgctg ttcgattatc ttgagctaag ccataagcta acccagtcaa aaaaaatacc
    13201 aggtaggcga tcaaaaacat cacccctgca actaacgcat aacgtaactt tgaatgcata
    13261 atttcattta atgctaaaaa catttaactc ctcctcctga aataatcatt ttatacaaca
    13321 attttatcac ttgataaatt tttggcgaat attatgctca aaaaattctt ccgctatttc
    13381 attatatgaa ttttttattc tcgattgatt aatatttatt atatccccac acaataaaag
    13441 accgtgatat ttctcacagt cttttatcta ctctattgct atttgctctc ttagctagta
    13501 ctttaaaagc taactcattg aggtagtgaa aacgagaggt gtgttcagac agaaatccaa
    13561 aatcctcaac tgctagctgt ggaatcgtag ttttgtgttt cacgatatcc gcaagcaggt
    13621 tcaaatctga taataagaat tcttggctta tcagctgtcc gccaattatt tctaatgtat
    13681 ccggatggta gatcagcttt gcatttactg ttttgttttc ttcatcgtat tgtggaaata
    13741 gatcaaattc tcctgaacac gaaccggtat tttgttccaa gaatggcgct tcatcttctg
    13801 tcagccctac agttcctaaa aagtagtcta taatgactgc tgctgttatc ttttgtctga
    13861 ggtcgtaagc ggttggctga ccactcaaag ttagcgctac tgctcttgcc atatgtacag
    13921 ctcgagtaac taaaggtaaa aagagatgcc gtttattttt gtcaaaactg actgggacga
    13981 tatctccaat cgcatagatg tccggatcac ttgtctgcag atattcattg actatcacag
    14041 tctcatcctc atacaaagcc actttttctt ttaacaatgt cgtattagga cgagtttggg
    14101 cagaaaaaat gaccatatct gcctttattg cttgagatag aagttgtacg gatactactt
    14161 tctcttctga ttttttccaa tcgatcaaga attcatttgg ataaaaattg atttgcttag
    14221 tttgcagctg attttgaaaa ctgttgatca tatccttatc gaaatatctt gataaaacat
    14281 tcggcatata atcaactaga tgaataatgt atcctttatt tctcaaagca tctgctaatt
    14341 ccaatccaat ataaccagca ccgaagatca ctacttcttt tgcttctttt aaagcggcaa
    14401 gtacttgctt agattgcact agatttttat aggtgtagac attttcagct tcaatttgtt
    14461 tatacaaggt gacaggagag gaacctgttg ctacgattaa tttatcatag gttatggtct
    14521 cgttttttcc ttctttggat gcgaggatga gctgatgttt gtctggatca agttcagtta
    14581 ctctccattc ggtgatcagt tttgccccac tttcttgaag agtccgcatg ttatgggcaa
    14641 cttcagctaa actacgtatc tttcctttta aataaaggtt gattccattt gaaacatagc
    14701 ctagtccatc tttatgatct tcatcgatca gtaaaacttc tgctttcggt tgaagttttc
    14761 ttagatttag ggcagctgtg atgcctgcat gggaagcacc aatgatcacg attttcatgt
    14821 tttattcatc cttaaattca atttttctaa ataacattct tttaaaaatt ggttcaaata
    14881 ggggaaatca taattatatt cgttgctagt gaatacaaaa actttagcgg acttctgtga
    14941 cgggtactct ttcaattggc tagtcaaaat gatatcatac tgcttttctt tctcgtattc
    15001 gactaaatca tatttgatcg tattcagcaa ttgttcctgg atgatttctt tcacgatatc
    15061 cgccattagc ctttcataag gaagatcata agcaatttgc agacgtaatt ctaactgctt
    15121 gatcagcgtc cgcaaaatag atgcatagcg agcaaataat tcaaagcggg cattttcctc
    15181 gtctacttca gtaaaatcaa caatagcaaa tgtttgtctg accaatcttt cacataaaac
    15241 caggaattgt ttatcttcta cttcagttaa ccgctgtttt aatccttctt ggccaaaaat
    15301 attcatccat cctctaaaat aggcaaaatg agcgtgattt tgcatcactg tcaacgcaat
    15361 cttttgcgaa atctcttctg gaaaaacata aaaagagaaa tattcttcta tcgattgttt
    15421 aaacgttcga ttcaattgag ctactacgtc gtctttgtat ttttcgctta tcaaaaactc
    15481 ctggacatgc gagttatgtg tatctaatgt aaagttactg atcatgaaaa gataaaaaac
    15541 caacgtttca ttttgattcc aaggaaaggc atattgaaag aagtaacggg tcaaaaatgt
    15601 atggaggtcc aataaaaatt gtgtcgtttg aaacaatcca acaggaagat tgctataatc
    15661 tggtttggat tttttcgtac agcgcattcg cttcttcata atggaaagcc atatttcaaa
    15721 tttcaatcgt ccttcttctg taatagtaaa actaaactct ttttgcagca gatctaaaaa
    15781 agtgtttcct gataaatcac tgtattcttt tttgttttca agataaggac gtccaaacca
    15841 aaataattgg aaataaaaat aacaaatttg actttctgga ccggtcagcc gcccgctttt
    15901 tattttcaga tgaaactcat ttaaagaatc atttaattcc ttcactctgc gagacaaagt
    15961 agccgaactg atattaagtt ccatgacaag tttctgccga tcaaatgagc cattttccaa
    16021 cagataaaga agtatctgat attttgtaga atagcgcaag tagcaatgat aataaacatc
    16081 gactgctgct atatttccat gtctgattaa taaaatattc ccttcttgga taacaatttc
    16141 caattgtttg atattttcca agttagcttg taaataaaac agatcttctt tcaaggtagc
    16201 ccgatcaact gataagcggg taagtacgtt attaacggaa atctctcctt tttcctctaa
    16261 taaaagttgg aaaagtttga tttgccgttg ttcatttttt tcaagcaaac tgaaaagttc
    16321 caagtccact cctccaagca tttctgattt taacagcaga agtttccttc tcatccttga
    16381 aaaagaggat ctgtgacagt tatcacagat ccatacaaat tctaatcact tagctggttg
    16441 gcccagctgt cagttcccat gtgaccatgc cttgatacgt tgcgccaggt tgagcattcg
    16501 tactgctatt tacctctaag agtaaaccat cggtatcggg aaagtcaacg agaacctctc
    16561 ctttagctgt tccatcactc tcataaatca acgtgttttg accttgctga atcaattgtt
    16621 ttgatccaga atgattgaag tataattttg ttccgcttaa ttcttgacct tgatcattct
    16681 taaataaaga agtcaatgtc cctgtcagtt gccaaggaga cgtgcttgga agccgagtat
    16741 cccgcacatc aaaagaaaaa tcgccttttg cttcataata acttgtcttc aacggtttgg
    16801 ggagatcgtt accaaatgta aaatcatctg gtacattaac taatgttaat tcttgtttgg
    16861 ggacaagaat tgttttagca gatgtttgga caactgggtt aggaagctta gcaccatctg
    16921 ctaatgtaat attgctggaa agtacagctg cctttgtcag tatgctgacg gattgtacag
    16981 aagtatctac agtatttaat tgatagttgt aagtaagatg aatggtatct cctattttgc
    17041 tcaatagatc tgctgggaca gaaactcctt gttgaatgcc ggtatctgcg atagttgtac
    17101 catttaattt taaggaacca ttgatataag tagtattttc aggaagtgag atcgtgattt
    17161 ttttgtcggt tatcgcatcc ccagattttg ttaacgtgaa tgtcacattt tcttgaatcg
    17221 tatctcctga tagagcagac aggggatctc ctgtctcagg ttgtctgcta aggtttttta
    17281 gctgactatc tgcactcaca gatggaaaat tagccactgt tatttgagga agagtggtga
    17341 ttccagcttg ttcagactgc catttgtacg caaaagacgg atacatgtta gtaagtacat
    17401 cgtccaccaa gcgataatca aaataatatt gccctgaagc caatgcaggt agtgtagaag
    17461 tagctgtatt tacacccccg ttatttgatt ggatcgtttg gaaaagttga tctgtccatc
    17521 ctgtcgctaa tttttgggtt cctttcgaat aaagcctgta atggatcgaa ccctttttta
    17581 gttcagttgc tgaccaattg gccgtgactt ggtacgatgc agaagaaagg agctttcccc
    17641 actgactatc ggctgttaag tttgttggta aaaccgttcc gtctagcttg tactgctcca
    17701 atacctttaa agcaacagga gaaagcaatc cgccttgcgc atctttccca ctgtacacga
    17761 tattggctgt ctgattcaat ccacttgtgc ttgctggagt atgagtcagt attgctcctt
    17821 gtgtaacgtt ttttcctgta aaatagttga ctacgcgatc aaagcgacta aatggcaact
    17881 gatcaaaacg tccttcagtc agccccatcg tcaatgtccg actattggta gagcctggaa
    17941 ggttctggtt atttccatcc acgtattctg tggtatcagc aaatgtatta tattttcctg
    18001 ttgaatcact tgttgagatg taactattcc acgtatttat ctctgcactt gcagaaaatg
    18061 catatgctcc tggattcaag tttccgccag ttaatttagg ataaaatcta cctgcagctg
    18121 gagctaaaat cgaattgaac atacgatctt ggataatatt attcgaatca taaaaatcaa
    18181 cagaaaaaac ggccttttta gacatagaag gttgtaattt acccttggtc aaaattttgt
    18241 ttggactcgt atcatattta tctccgtttt ctgtcatctt cgcgacatta gctgtaacat
    18301 caaattgaag cgggatatcg tccacgttga attcgcctcg cgtaataata gcaataatat
    18361 ctgccaatag agtatttgta tcccacgacc ccatcccaaa acgcaaggta tggcttcctg
    18421 ctgataataa actaccgcta gtaaccttga tgggggcacc ggcaatatac aagtaggcgt
    18481 tgttccagtc aattgctttt aatacatcac tgcttgatac atgttccggc aaattgatga
    18541 tcgccacaaa tgcaaatcct tgtgcagtga tattcaccaa atcaagtaca tttgttagtt
    18601 tcacatggct ttcccaatga aaacggaccg tgccaccttt ccaaggatca tttgaaccat
    18661 cgttcaaata agtagcagtc aatttaggca attggttagc cccagttagt tggacaagct
    18721 tcccgccatt gactgcacta cgtccaccat ttggaggtac gatattttca tacgtgtatg
    18781 gatcataaga aatggcagga ggcgttgctc caacagcttc tgccgaaggt gaaaagataa
    18841 atatacttaa aacaaaaagc gaagtaaaaa agaaggagcg aaaaaatgaa aatttatttt
    18901 gtagcaacat gttcatcctc cttttccttt cttctccata acaacaacca cgcaagaagc
    18961 cataattcta taccaatgat caaataaaga aaacggatct tttcttcatt cgtctccggg
    19021 aatacgccat gttgattaga ttctttataa atcggtagct gtgtctgctg ttttgatgcg
    19081 ccattgactg aaccacttgg atgcggaact ggacttagat cataaaatgt aatcgtacct
    19141 gaacttttgg tatctgcttg gactggctct tgccagctga agaacagcaa acttgcaata
    19201 atccctccca ataagatcga tgttttcaac gaaatcacct catcatgttg tttctccttt
    19261 tttctttttc ttgtggaccc acaaaaacag caaaatcaac cataacacta gaactgcagc
    19321 aaccaatcct gcataaagcc atgggaaaga ttgttcttgt actgcaaaaa cggaattttc
    19381 atttactttt tgtgcttttt cttttttgat cgtaaagttt tgtgtaaatc gccattcatt
    19441 actgtcgcct gattttgcta tgatttctgc gcggtaatcg ccggcttttg ctttatcacc
    19501 agctaaatca agattcaagt gaaaattcga atttggtgca actaaaaatt cattagacgt
    19561 attttcaata taaagctgat tttcataaaa gatcttgctc gtaaatatca aattgctgat
    19621 aatcctaggc tgaggatttt ggaaacgaag ctgaacagtt ggctttgcgt tgacctcgtc
    19681 caaatctcct gataaaagtt tcagatccgg ttctatcgtc tcttgacttt cttgtaaaac
    19741 gactgcgaga ttataagcaa tacgattttt gatttgtgct ccttctgtag cattttctgt
    19801 ttcagcttct tctttaggtg aaacatgaat acccgctaat acacgtccct taaagctttt
    19861 tgctgggatt ttcacttcta taggtatagt tgtttctgca tgtgctgata cattgatttc
    19921 ctttttcgca atgagcaaaa cgtcacggac atcaaacggc aatgtctcat ctaacgttgg
    19981 gtttttttga ctataatcga tcgttccacc atcactcgtt accgccggat tgacagagac
    20041 ctcaaatgtt cgttcttcat ctgaagaatt gctgataatt acttctagtg tctgattttg
    20101 ttccggtgcc actaataaat caaaataacc gattgcttta tctatttggt tctctggaaa
    20161 taccggtttg attccaaagt ctcctgctga atcctttgct tggctgattg acggaaaaat
    20221 gaaaaataga cttaaaatag aaaaaaacga aacaaacagt cgtttacttt gattcattgt
    20281 ccaccacatc ctttcaacaa caactgcggt aaaaaatcct tcacctatag tattttcacc
    20341 gcagtttgtt gaatttattt catactctat gctactgggc cagccacgag gttccaagtc
    20401 aaatcagccg tgaatgttcc agctgctgct ttcggcactt tcagtgtgat atttgctgga
    20461 tcataaacag aaagccatgt tcccagacct tgatcttttg ctgctgtaaa tattggtgtc
    20521 gctgcacttg ctttactcaa cccagttaca gtttgactag ttggtccagt ggattcgtta
    20581 tctgacgtac ttttaacagt ggatgttcca aaatctaatt cagcgccttg caaaacttgt
    20641 gaaccgttta caaaatctga aaccgaaact gtcacgttcc acccttgtgc ttggccatct
    20701 gcacctacac ctcgacgatc tgatacttgg agataaggtg tatcgttttt tgaagcgtct
    20761 acttgatacg tttttgttcc gctttcaatc tcgtgtgtac caaatggcaa ttcaggaact
    20821 acatccaatg ttaaagaacc tgtctggcca gttgctggat tttccggatc tgctggctct
    20881 actggttttg aaggatcagt agggttaaga ggctcaacgg tatttgttgg ttcagtaaag
    20941 gtaatatctc cttttgaagt cgcttgaccc tcagcaaaag ctggtgttgc caagccgcct
    21001 agaagaagtg ttgaaagtaa taatgtccca atcatgttct ttttcataat aaatcactcc
    21061 tttttcttag tgacctaagt atagtagaga cagtatgcaa aaaatcctca tgaattcatc
    21121 cgcttacttg aaattatgac cctttttgta taaaagagaa aaaaacaata aaatcgttta
    21181 catgaatatt ctt
//
LOCUS       DAZUKZ010000040        20586 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000040 DAZUKZ010000000
VERSION     DAZUKZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.040"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="ACG8EF_001433"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="ACG8EF_001433"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFS4946746.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="ACG8EF_001434"
     CDS             1011..2258
                     /locus_tag="ACG8EF_001434"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFS4946747.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="ACG8EF_001435"
     CDS             2334..2678
                     /locus_tag="ACG8EF_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946748.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="ACG8EF_001436"
     CDS             complement(2795..3121)
                     /locus_tag="ACG8EF_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946749.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3736..4938)
                     /locus_tag="ACG8EF_001437"
     CDS             complement(3736..4938)
                     /locus_tag="ACG8EF_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4946750.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            5055..5570
                     /locus_tag="ACG8EF_001438"
     CDS             5055..5570
                     /locus_tag="ACG8EF_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4946751.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5769..6215)
                     /locus_tag="ACG8EF_001439"
     CDS             complement(5769..6215)
                     /locus_tag="ACG8EF_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS4946752.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6262..7740)
                     /gene="msr(C)"
                     /locus_tag="ACG8EF_001440"
     CDS             complement(6262..7740)
                     /gene="msr(C)"
                     /locus_tag="ACG8EF_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS4946753.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(8094..8723)
                     /locus_tag="ACG8EF_001441"
     CDS             complement(8094..8723)
                     /locus_tag="ACG8EF_001441"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4946754.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(8707..9171)
                     /locus_tag="ACG8EF_001442"
     CDS             complement(8707..9171)
                     /locus_tag="ACG8EF_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4946755.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9658..10926)
                     /locus_tag="ACG8EF_001443"
     CDS             complement(9658..10926)
                     /locus_tag="ACG8EF_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS4946756.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(11157..11894)
                     /locus_tag="ACG8EF_001444"
     CDS             complement(11157..11894)
                     /locus_tag="ACG8EF_001444"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4946757.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11961..12530)
                     /locus_tag="ACG8EF_001445"
     CDS             complement(11961..12530)
                     /locus_tag="ACG8EF_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFS4946758.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12658..13530
                     /locus_tag="ACG8EF_001446"
     CDS             12658..13530
                     /locus_tag="ACG8EF_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4946759.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13872..14744
                     /locus_tag="ACG8EF_001447"
     CDS             13872..14744
                     /locus_tag="ACG8EF_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4946760.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14741..16699
                     /gene="nagE"
                     /locus_tag="ACG8EF_001448"
     CDS             14741..16699
                     /gene="nagE"
                     /locus_tag="ACG8EF_001448"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFS4946761.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(17031..17882)
                     /locus_tag="ACG8EF_001449"
     CDS             complement(17031..17882)
                     /locus_tag="ACG8EF_001449"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4946762.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            18005..18472
                     /locus_tag="ACG8EF_001450"
     CDS             18005..18472
                     /locus_tag="ACG8EF_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFS4946763.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18735..19217)
                     /locus_tag="ACG8EF_001451"
     CDS             complement(18735..19217)
                     /locus_tag="ACG8EF_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4946764.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(19317..20180)
                     /locus_tag="ACG8EF_001452"
     CDS             complement(19317..20180)
                     /locus_tag="ACG8EF_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4946765.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6197 a         4227 c         3724 g         6438 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgat cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca atcagctgac cctcggtatt agctcaaaaa acggtaaaat
     3421 atgagaagct atttttccgt tttgtttctc taatacttag gataaaacgc tgattttaca
     3481 acctctgtta aacggtgttc aatcagctgt ccttcggtat tagctcaaaa aacggcaaaa
     3541 tatgaggaac tatttttccg tttgtttctc taatactcag gacaaaacgc tgattttaca
     3601 acctcctgat aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3661 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3721 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3781 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3841 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3901 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3961 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     4021 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     4081 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     4141 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     4201 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4261 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4321 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4381 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4441 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4501 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4561 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4621 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
     4681 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
     4741 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
     4801 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
     4861 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
     4921 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
     4981 tcaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
     5041 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
     5101 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
     5161 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
     5221 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
     5281 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
     5341 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
     5401 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
     5461 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
     5521 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
     5581 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
     5641 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
     5701 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
     5761 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
     5821 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
     5881 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
     5941 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
     6001 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
     6061 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
     6121 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
     6181 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
     6241 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
     6301 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
     6361 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
     6421 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
     6481 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
     6541 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
     6601 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
     6661 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
     6721 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
     6781 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
     6841 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
     6901 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
     6961 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
     7021 cagtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
     7081 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
     7141 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
     7201 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
     7261 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
     7321 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
     7381 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
     7441 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
     7501 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
     7561 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
     7621 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
     7681 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
     7741 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
     7801 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
     7861 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
     7921 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
     7981 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
     8041 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
     8101 aacatagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
     8161 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
     8221 aactgatctg cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact
     8281 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
     8341 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
     8401 ggcatcttcc ctgattcgta agcttggttg tagatttctt cgccatattc gtaacattgc
     8461 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
     8521 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
     8581 ttcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
     8641 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
     8701 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
     8761 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
     8821 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct tgattttctt
     8881 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
     8941 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
     9001 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
     9061 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
     9121 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
     9181 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
     9241 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
     9301 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
     9361 tattgattct tatcaaacta tagaagatag ctcttctgca gtagataatt ttacaccgcc
     9421 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
     9481 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
     9541 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
     9601 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
     9661 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
     9721 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
     9781 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
     9841 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
     9901 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
     9961 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
    10021 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
    10081 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
    10141 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
    10201 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    10261 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    10321 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    10381 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    10441 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    10501 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    10561 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    10621 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    10681 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    10741 aattgtattt tctgagacag tagctgactg caaagtactt tctgtatcgt tttcaattcc
    10801 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    10861 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    10921 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    10981 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttaa taaatttgct
    11041 ttttctgcat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    11101 ggtcgtttga ataactattt aacaagtaat tctctctgga tattatccgt tatggattat
    11161 acttcctgtg tcgtcggacg gataatcatg ttgctgacag acatccggtc tggcgtttct
    11221 atcgcaaatg ctacagcaga agctatatct tcagatgtta atccccattc tttttgcgct
    11281 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    11341 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    11401 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    11461 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    11521 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    11581 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    11641 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    11701 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    11761 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    11821 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    11881 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    11941 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    12001 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    12061 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    12121 tttttcttga tgtggattgg ctttactgac aagcctcttc gttcttttgg atcggttaga
    12181 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    12241 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    12301 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    12361 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    12421 gctgctaaag gctcttcgtt ttccgcttgg atctgatgga tcacttcaaa gagcggatcc
    12481 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    12541 ccttttatcc ttactttata acggacggct tatgatgtct aatacttata aaacatactt
    12601 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    12661 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    12721 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    12781 gaacttggtg tgacactttt cactcgaggc tcccgagaga tacagttgac ggattctggt
    12841 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    12901 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    12961 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    13021 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    13081 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    13141 caagatcgtt ggggactttt gatacatcgg aatcatcctt tagcaaagca aacagtcatc
    13201 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    13261 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    13321 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    13381 gggatcatca atacttatgg gacggaaaaa gtattcatcc cactcgatcc gccattacaa
    13441 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    13501 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    13561 ttaacaacga ataagttaag caaaagatcc aatgattaaa taacataagt ttcttaattt
    13621 gttccttcct tttatttctg ataattccag taaattacta taattataaa aaaagaattg
    13681 acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact
    13741 agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg
    13801 tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa
    13861 agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg
    13921 gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt
    13981 tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt
    14041 tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg
    14101 atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg
    14161 accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt
    14221 taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt
    14281 atctgacaaa agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc
    14341 atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga
    14401 ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac
    14461 cattttcact gaattattca agactggtga atcatttacg actgcttcta caacgaaccc
    14521 atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc
    14581 cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg
    14641 cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa
    14701 tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg
    14761 aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg
    14821 gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg
    14881 cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat
    14941 gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt
    15001 aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga
    15061 tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc
    15121 agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg
    15181 caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact
    15241 gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt
    15301 aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt
    15361 gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa
    15421 atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc
    15481 aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga
    15541 aaacaaagca aaaacagctt ccttgatgtt agcagcagct tttgcttcat tctttactgg
    15601 tgtaacagaa ccacttgaat tctcatttat gttcgttgct tggccattat atgtcttgca
    15661 tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt
    15721 tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca
    15781 gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa
    15841 ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga
    15901 tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta
    15961 cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg
    16021 tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc
    16081 tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt
    16141 cgttgcagat gagatggcaa aactacatgg tggtgctgca acacgacctg cacaaacaaa
    16201 caccgtggtt aaaacagaaa catttgccgg tggaacgatc gaacaagata tttacgctat
    16261 cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat
    16321 gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg
    16381 tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat
    16441 cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt
    16501 atatgtaaaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc
    16561 cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa
    16621 agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc
    16681 tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag
    16741 gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc
    16801 caactccgcg gtaataagtc cagccaaacg taacatatga agagctattt tttatttgac
    16861 tcttcttatt acttgatgca gaacagcttt tttacaacct ctgataaacg gtgttcaaaa
    16921 accagtttat cggtatgaag tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa
    16981 tttttcttca tactcaaaac taatcggctt tttcacaacc tctttataca ttattcctga
    17041 tccaatattt cttttaatgc tgcttcaaga gaagttaatg gttttcccaa tactttttct
    17101 aaatcatcag aggaaatagc taactgatcc tttttaatat catgttggat cgctaaaaac
    17161 atgtcagctg cttcttgagg gaatccggct tcttttagat tttcgacaaa cgctgcatca
    17221 cttgcttcaa taatttctaa ctttttacct gtcgcacgct ctaatgcctt ggctaattct
    17281 tcatagtcag ttagattgcc ggataattct aagatttctg gagagtcact ttctactagt
    17341 acttttgctg cagcttctgc atattcacgt ttcaatgccc aacctgtttt tccatttcct
    17401 gcagcataaa cgaattgacc agtttttaaa gcctgcccaa tgataggtaa ttcattctct
    17461 acataccaat tattccgtaa aaatgtatgg gaaatacctg aatccttaat cattttttca
    17521 gtgaattggt gatctggcgc taatacactg atcgagtgat cggcatctgc aaaacttgta
    17581 taagcgatat aagatacccc agctgcttgt gctgccttca ctacattctt atgttcggct
    17641 tgacgatttc ctggaacgcc agagacaaaa agcaatcgat ctattccttt caacgcttct
    17701 ttcattgagt ctaaatcatc ataatcacca atacgtatat taaatccagc ttctttcagt
    17761 gcttctcctt tttcttcact acgtactagt acatagattt cattttgtgg aaccatcttt
    17821 tttaagaatt ccagcgcata accaccaaaa cctccagttg ctcctgttac taaataatgc
    17881 atatttgcca ctccctattc tttttatttg ttaagattta cagctgtaca tttataaatt
    17941 acacctttat cataaagagt gtggtaagat aagtcaagca aaaaaaatga aaggatgaca
    18001 actgatgaag tattctctgc aatttagtga tgcaatccat gtacttgcct atattgaaat
    18061 gtttcaagat acccgatcac tttctagtga gatgattgcc agtagtatcg aaacaaatgc
    18121 agcaaatgta cgtaaaatca tgagtcagct caaaaaatcc ggtttgatct ttacaaaaac
    18181 cggaaaaccg cagccttccc ttgccaaaag tccagatcag atttctttac tagatgtcta
    18241 tcgcagcatc gaaggtaata cacagcttat ccaagtcgat ccaaaaacaa atccaaattg
    18301 cgtcgttggt gcgaatatcc aagaagcttt agaagaaagc tacgctcggc tgcagaaaaa
    18361 ggcagaagaa gaaatggcta agatcacact tgatagtctg atacataaaa tagccgtttt
    18421 ggaaaaagaa aaaagaccag aaaacacagg cctcgtagag agatttttat aattctctct
    18481 actgtgcctg catttttctg atcggaatcg tctacataaa ttcttttatt ctccggggga
    18541 caaaagcctt gtcatgcccc tttttcctct ctaatgctca ggtcaaagcg ctgaagtcat
    18601 gacgtcttga taaacggtgt tcaagtagct gaccctcggc attagttctg aaaaatgcaa
    18661 aatatggaaa gctatttccc atttttcctc tctaatgctc aggtcaaagc gctactttca
    18721 cgacctctat attatcatgg tttattgact accggtatcc agatttctcc catatatttc
    18781 ccgttggtaa ctcccatttc gactacagca tttggacccc caacataggc gtaatcttct
    18841 acttctctta aaacatgccc aaaggccgta ttcgtcaaat catttcttaa ttcttcaggc
    18901 gattctgcta aagcgattac aacaagatac gttccttttg ggaatgggat gattcgggta
    18961 gtgtttggaa catcctgact cgtttgaaca ccgacatagt acatcatttt cccgttatag
    19021 ggttcgttga ctgtaaagaa ataatgattt tcagcagctt gtttcaatgc atctattgtt
    19081 ccattaccga taactttttg ctcaaatgct tgtttctctg cagccaaacc gacaaaatcc
    19141 gtattgctac tcttcaattc cacaccaata cccgtcaccg taaaatctgg tttttcttcg
    19201 atcctatact ttgccatttc tcccaccctt tctattatat taagttcatc ttacataaaa
    19261 ttaaagggaa atgacacgat tacgctcttt tttctaggga tatttttatt ttttaactat
    19321 ttttttgctt tcactgcaat ccaaatctca ctgtaaacat tactgaaatc ctctccccat
    19381 tttgtaaaag agatctcggg agcttcgact aactcataat ctgaagaagg aagccattct
    19441 gcatagattt tcccccaagt atcttgtaaa gtttggggaa atggcccttt attaggaaaa
    19501 atggcccaag tgtgttcttc tatagcaagc atttctaaat cagcgtacgg attttctttt
    19561 gtcgtagcaa agccaatcat gtgcatcaaa ctgccctttt cttccaagcg ggctccatca
    19621 aatccataag aaacgttgat tacttgattt ggatataaat ctcctaactt atgcatctct
    19681 tccctttgag tctccgtgat cgtctgcgct aacttttgta tttcttgatt gactccttca
    19741 aatttgacag gaactttttt cgccacacct gccacataaa actgttcttt cttttcgata
    19801 cgaaattcca tcgattttcc tcctctgaca tcaataaaaa atgtcaagcg tggaaaagat
    19861 ttttgtatgc cgtatctgct gatttctgaa ggcagatatc ctgaccaatc acgaaaagct
    19921 cttgaaaaac cttcgactgt ttcatatcca tatctaaacg ccgtttctgt cactcttgat
    19981 ccatctagga gatcgcgatt agccaaagcc aatctacggt ttttgatata gtccattagc
    20041 gacatccctg caataaacgt aaagatgcgt tttaattgat attcagagat tcctgtattt
    20101 tcggctatta ttttatagga aagtccttct gtcaaatgtt cttcgatgta atcgatcaat
    20161 tggttcagat ctttaagcat atttatcttc ctccttgctt gcctttttat catattatca
    20221 aagaaagtca attacttgat gaatcaggaa ccaatttagt cagatgtaga aaatgttgta
    20281 tggatctaaa gaaaattctc aagcagcgtt tcgtaacgga taattggtat tatctgccta
    20341 tctatgtttt acctttagaa aaataaattt tggggaagac acattctaag taaacaaagc
    20401 ggtaaaaata tcttttattc ccttagttaa tccgcttact acctactgta caaagcaaat
    20461 tgttgttcaa actgctgatc agaacgctgc ttggattttt ctttgcgata catcatacct
    20521 cttttatcaa ttggaacaat tatttatttc taacaaaatc acttcatttt tgtcagataa
    20581 ataaaa
//
LOCUS       DAZUKZ010000041        20500 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000041 DAZUKZ010000000
VERSION     DAZUKZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.041"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..561)
                     /locus_tag="ACG8EF_001453"
     CDS             complement(<1..561)
                     /locus_tag="ACG8EF_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFS4946766.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(748..1398)
                     /locus_tag="ACG8EF_001454"
     CDS             complement(748..1398)
                     /locus_tag="ACG8EF_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946767.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="ACG8EF_001455"
     CDS             complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="ACG8EF_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFS4946768.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2910..3908)
                     /gene="yvcK"
                     /locus_tag="ACG8EF_001456"
     CDS             complement(2910..3908)
                     /gene="yvcK"
                     /locus_tag="ACG8EF_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFS4946769.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="ACG8EF_001457"
     CDS             complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="ACG8EF_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFS4946770.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4924..5655)
                     /locus_tag="ACG8EF_001458"
     CDS             complement(4924..5655)
                     /locus_tag="ACG8EF_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFS4946771.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5659..6924)
                     /locus_tag="ACG8EF_001459"
     CDS             complement(5659..6924)
                     /locus_tag="ACG8EF_001459"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFS4946772.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="ACG8EF_001460"
     CDS             complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="ACG8EF_001460"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFS4946773.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="ACG8EF_001461"
     CDS             complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="ACG8EF_001461"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFS4946774.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12266..13768
                     /locus_tag="ACG8EF_001462"
     CDS             12266..13768
                     /locus_tag="ACG8EF_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFS4946775.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13770..14507
                     /locus_tag="ACG8EF_001463"
     CDS             13770..14507
                     /locus_tag="ACG8EF_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4946776.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14552..14725)
                     /locus_tag="ACG8EF_001464"
     CDS             complement(14552..14725)
                     /locus_tag="ACG8EF_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946777.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14737..15366
                     /locus_tag="ACG8EF_001465"
     CDS             14737..15366
                     /locus_tag="ACG8EF_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4946778.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            complement(15448..16614)
                     /gene="dnaJ"
                     /locus_tag="ACG8EF_001466"
     CDS             complement(15448..16614)
                     /gene="dnaJ"
                     /locus_tag="ACG8EF_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFS4946779.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16765..18594)
                     /gene="dnaK"
                     /locus_tag="ACG8EF_001467"
     CDS             complement(16765..18594)
                     /gene="dnaK"
                     /locus_tag="ACG8EF_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFS4946780.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18645..19208)
                     /gene="grpE"
                     /locus_tag="ACG8EF_001468"
     CDS             complement(18645..19208)
                     /gene="grpE"
                     /locus_tag="ACG8EF_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFS4946781.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19302..20345)
                     /gene="hrcA"
                     /locus_tag="ACG8EF_001469"
     CDS             complement(19302..20345)
                     /gene="hrcA"
                     /locus_tag="ACG8EF_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFS4946782.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
BASE COUNT         5823 a         4459 c         3504 g         6714 t
ORIGIN      
        1 tataaacatc accccaaata aaacagaaaa cagtataagc attataaaaa ttatcaacaa
       61 ccttgatcct tcttttatag attcaggaaa cagtataatt ttagtactag ataaattgtt
      121 tacaccgatt agtgttaaaa tatctttatt cttcttgcta acataaccct ttatgcacaa
      181 aagaacagct atacttaaag taacaaataa ataacgtatt ccaatagaaa aatcactata
      241 aatactctct agcataggac cgacaattga agaagtcact actgcaaatg ctggacctat
      301 ccaagcaaca ttagaatgtt ctttctctct tttcctttgt aaatctttgt tgagtattaa
      361 tctatttatt tcttctgcag gcaagttaat tttcataacc ttatggggga ttatccaatt
      421 aaaaatcgga aaaaggtagc ctccccaaaa agtatagtga tctgtgtcaa tcagataata
      481 acctgatttt gattccagaa taacatatct caaagccgaa cgaaaatttt tcatgaatat
      541 ccataatctc actgtttcca cacctaattc ctccaattcc aattaaatgg atttatagca
      601 tcaccaatta tgttaactgc ctgaccaacc tcattgataa aattggcacc actatcccat
      661 gcttcaacaa tgctatatat ctatcaaaca aaaaatggaa aactaaatac tcgcgcacat
      721 aaattttaca ttctgttttt tataagacta ataataatca ttgaaaaaat ttcttatcgg
      781 tggcgcatat tcccaattga ttaatgtgat cccatattct gcaagtattt tacgagcttc
      841 ttctaagtca tagtgagaaa aatgcataaa acctgcaata cgatcacgga ctgtgagctg
      901 tggcttgctt ccaccatcat tcataaaata tccagcagag ccactactta aagtgattgt
      961 tacatcttca ctttctatta ttaagaattc ataatgaaat gctttcaact ctccactatt
     1021 atttaaaagt tttatactcc aacttcgtct aggttgagtt gtcacattcc ctctaaaact
     1081 tcctataaat tctgcaatca ccgtatcttc aatcaaaatt ctttcctcat ctatttcata
     1141 ttcaagacta aacggaaatt ctccatatgt aatctctggc aatggtggtg ctgtagttct
     1201 ccaaataaat acaataactg tgaatagaaa aaatccaacg atcgatataa atgttatgag
     1261 acctgctcga agtaaaaccc gatataataa aaatgctgta tttcgctttg ctatatctag
     1321 aaaaataaaa agtactgcaa tcataatgat agtaaaagtt gtaaaaaagc acattttttg
     1381 ccctcccttt ataaacatcg attctcaaat tctaaaccaa atcttgatag ttcaagttca
     1441 ggtataacgt ataccaacca tacatcccaa ttactattta tgaacttatg attatcactg
     1501 aataaatcac tccatcccct tgttaaaact tctacattaa ttacacctgg ttgttttcga
     1561 ctagctaatc caatgaactt atcaaccatc cttatccacc aaccacgaga atcttgacca
     1621 gaagtaagaa aaacagttct caaggttgat cgagcagttc cttccagttt ttaattaatc
     1681 tccagttata gataaatact tgatcctatc tatatcagga cttctatatt aagcaaaagt
     1741 ttatgtataa aactaaaatc ttcattcagt tgtctccccg ctttttatac gattgtatta
     1801 aaaaacaata gaagcctttc aacattatgt tactccagta aagttggaat tatagtttga
     1861 cggaagaaaa gcaagtatag cttttaaaaa aaactgatac attcgcatct cctgcttcat
     1921 gtatcagttt tttctttgga ttaaactgct tcttcacgca gtttttccgc aaattcattt
     1981 attttgcgaa ttcggtgatt aattcccgat tttgatattg cacctgaggg aatcatttcc
     2041 cctaattctt tcaagctgac ctctggatgt tgaagccgaa gttctgcgat ttcctgaagt
     2101 ttttcaggaa gggcatgtag accgaccttt gcttcgatca attcgatgtt ttcgatttgt
     2161 ttagaagccg catcaatcgt cttgttcaga tttgctgtct cacaattgac tagacgattg
     2221 accgaatttc tcatatcccg tacgattcga acatcttcaa atcggagcat tgagttggtt
     2281 gcaccaatca atgttaagaa atcagcaatt ttttctgctc cttttaaata cgaaatatag
     2341 ccattgcgcc gttcaagcgt tcgagcatgt agaccgtaat aatttaacat atcgcaaatg
     2401 tcttggttat gctgttcata aatagaaaat atttccaaat gataacgact cgtttccgga
     2461 ttattgactg aacctgaggc cataaaagca ccacgtaagt aagaacgcat tttttgcgaa
     2521 tttcccataa tctcgtccga aacgtgtcca ttgaacatca taccatccat gatatcaaga
     2581 tccatcaaca catcttttgt gccttgcttc aaccggacga tatagacatt gttctttttt
     2641 aatttcattt ttttacgaac gagtagctca cttcgtacct gataatgatc tttcaaaaga
     2701 gaatagatac gtctggcaat tgcagcattt tctgtttgga cgttcaaaat aaactgctgg
     2761 ttcacaatac ctaaagagcc gttcattcgc agcaaagcag ctaattccgc tttcgcatgt
     2821 tctcgatgaa cttccaaacc agttagttct ttcttcacat ctgcagcaaa tgacatccta
     2881 ttctcctcct ttctccaaca ataaattgct taatattttg aaccgaaaac taaacggaac
     2941 aattcttcta ctactttgtc tccatcgtgg aagacaccac catctttcaa ttctaaaaag
     3001 tcggtggaaa tcactcgaca tccttcattt ctcaatccgt tgaagtcgtg tgcgacttgt
     3061 actagatact catcatagat ctctgggtcc atataatctt caggcacttt ttccgtattg
     3121 accaatactg tatcgataaa aggctgattc aaatgcttgt gtaaaacacg tatatgatcg
     3181 gcatctgtga aatgttccgt ttcccctttt tgggtcatga tattacagat atagacggtt
     3241 tcagcagttg tcttgagcat tgcttcgccg atttctggta tcactaaatt tggaaggata
     3301 ctggtgaata aacttccggg tcccaaaacg accatgtcgg cgtccataat tgctgaaacg
     3361 acttttcgag ccgctttggc ttcactttta tcatttgtgt tgcagacata cacacggtca
     3421 atggttttac ggttgatcgc gattttagat tctcctactg cagtcgttcc gtctttaaaa
     3481 acagcatgca aggtcaaagg agtttctgaa gaaggataaa catgtccatc tacatgcatc
     3541 atttttgcta ataattgaat cgcctcataa gtgcttcccc gcatctcgga aactgcggca
     3601 atgatcagat taccgatcgt atgattggct aaaaactgat cttccttttt gaaacggtat
     3661 tggaaaatat cttcatagaa ttgtggcata tccgataacg ccactaagac atttctcaag
     3721 tcaccaggag gagccatagc gatcgattct ctgattgcac cgctgcttcc accatcatct
     3781 gcaacagtca ctactgctgt gatatctgct ccttgatttc gtaaactttt caggataact
     3841 ggcagaccag tgccaccgcc gaccactacg atctttggtt ttcttatccg gtaggttttc
     3901 attttcacga ccggttcacc gtctctttcc ttttatcctt gtcccgatgt gtcacattga
     3961 ctttatagtt tttccctaaa gcatttgcca aacgttcagt caatgccaca gaacggtgtt
     4021 gaccgcctgt acagccgatt gcaactgtca agctgctttt tccttcttta atgtatccag
     4081 gcaatacagt ttccagaaga tccaaaaatt tatggtagaa tgattgcgtt tcatcaaagg
     4141 acatcacata atcatagact ggctgatctt ttccggtcaa tggtcttagt tccgggatat
     4201 aatgagggtt gggcaaaaaa cgcacatcca tcacaatatc cgaatcgatc ggcaacccgt
     4261 atttaaaacc aaatgataca aattcgatcc gaaatccttg gtctcccttt gatttgaatg
     4321 actcattgat tttttcacgt aattgtcgtg gtgtcaatgt tgtcgtatca atgatcacgg
     4381 aagcttgggc tttcaagtcg tctagtatcg cccgttcttt acgtatccct tcagtgatca
     4441 aaccgtccat cgccattgga tgtgctcgtc ttgtttcttt gtagcgagcc actagttctt
     4501 cgtctgaagc atctaaaaat aagatagaag tgtcgatgaa gttcgtattt tcgatttcga
     4561 tcagcatgtt ttggatttct tcaaagaagg aacgagaacg caaatccact cctaaagcca
     4621 tttttgtgac tttacctgat tctttgatca attcccagaa ttttggaatc aatcgaggcg
     4681 gcatattatc gatacaaaaa tagcccatat cttcaaaact ttggatagct actgttttcc
     4741 ctgcaccact catacctgtt atgatgacta attgcaaatt atctgtcata tttacgaacc
     4801 cctctcacat tgtgcataat aaaattatat catcccgggc gtatcattac cagttttctt
     4861 tttcttttac aaaaagattt ggaccaaata tcttttagga tatttgcccc aaatccaatt
     4921 tttttacttt tccgatgctg tatccaatgc ctcgcttctt tcagctaatg cacgttcgat
     4981 cgttcgatcc gctaaaatag attgagcatc gatcactggg taatgattat cttcagcaaa
     5041 ttcatgcatc aaagataatt ctgtgcaacc taggatgact ttttcacagt ttaggcgctc
     5101 taccgcttcc tctaagattt cgtaatagag ctcttgattc aaataatccg attccttgat
     5161 ctcatgatag atcaggtaat tgattttttc ttgaagcgca gtatcaggaa tcactgtttc
     5221 gaaaccaaga tttttgactt cgcgttcata gattccagct ttcatacttc cttctgttcc
     5281 tagaatcgct actctccctg tcgtatgctg acggacaagt tcatttgctg cttctcttgg
     5341 catatgcaag ataggaatat ccgttgccgc ttgtaactct tcaaaaaagt agtgtgcggt
     5401 gttacaagtc aacacaatga agtttggtcg caataaattt tgtttctcaa tatcatccag
     5461 cagaaacggc attggatttt cttctgatcg atctaaaata taagctgtac gatcaggaac
     5521 tgttgcatga ttgaataaga cataattcaa atattcttga tcttttgtag ctttcgttcg
     5581 gtggttgatc aagcgaacaa aactttctgt cgccatcgtg cccattccgc ctaaaatact
     5641 gaaaaaattc tccatgaact agccttcttt cacatgaaaa tactttttga atcttggaat
     5701 atagttatgg aacatatatt tcatcaacaa ccagcgcttg attgatcggt ctttttcata
     5761 aaatacagta gtaccgtaac gattttcttt gatcatttgc tcagctagtt ttttgtcttc
     5821 attttcccga gcatattcta aaaagatttt tttgggcaca cccatccata atcttgcttt
     5881 atctgattga ttcgttccat aggtcgtttc aacaaagggt ttgttgaaca ctcgatcttc
     5941 tgtgacaaaa cgagccagat tcaagccgtt caatgtcaca aagaaactgc tacgaccttg
     6001 tctcaaattg atttcaaata atttatattc tccatctcta ggatcatact tcatatcgaa
     6061 attggcaaag cctgtatatt cgattttttc caaaaatgcc ttaatggttt gatagatttt
     6121 ttcgttataa tctggcatga tcacaacgta attaccaatc gatgctggtg taggatcttc
     6181 taatagtgga tgaccaaggc acatcatccg aacttgatga tcttcatcta catacgcatt
     6241 tagcacacgc atattactat catcacccgg gataaaatct tgtacaatca tttcactttt
     6301 ataaccggct tcgtatatcc ggcctaaaat taaatcgaac tcttctctag tctctaaaat
     6361 aaaagctttt tttcttcctt caaattggac agaaagatac tccacactgt ttgcaggttt
     6421 caaagcaact gggaattcaa aaggcaattc ctgttccaaa tgtccattta ctaacatttc
     6481 ttctctgacg atcaatgttt taggatacgg aagatcatat tcttcacaaa cttcataaaa
     6541 gcttacttta ttgatcagcc tttcaaataa agaataatca atatatggac agataaaata
     6601 ttctgacagc tcttgtttat gttgggagat caattctgca tatccatcac cacaagcaat
     6661 caaaagatat tttttactct tatcagaata tctttctttt gccaaacgaa gcatcgtctc
     6721 gataaataca gggtctttat caaatcctgg aataacttct acattgacaa ttttactgta
     6781 gcgggtaggg gctaactgat ccgaagcata tgcttggcag attttgccgt atgcttcatt
     6841 aaaagaacgt gccataccat aaacgttcat gtcgcttcct aataatatag ggataaattc
     6901 ttcattttca atactgttca tcattatctc tcacttactc ataatctctt gtttttcttt
     6961 tccaactaca caaggaatat cataccataa agaaaaaagc aatcaaagga aagtcctccg
     7021 attgctcaat tcttttttga acggattgaa cttataggaa gtttttatct ataccaatca
     7081 gtgtagaaag aactaccatg taatgattct agaacactga tattaataaa acaagcaaaa
     7141 attcattttt ttatgttttt ttaactatct cagctatcta tcctctttat accattattt
     7201 ttttcaaata gtgacctgta taactttctt ttacgttaat gatttcttct ggtgttccag
     7261 tcgcaaggat cgttcctcca ccttctccac cttctggtcc taaatcaatg atataatctg
     7321 ctgttttgat tacatctaga ttgtgttcaa tcactaaaac tgtgttacca gcatctacta
     7381 atctttgtaa tacatgcaac aagcgggcga tgtcatcgct atgaagtcct gtcgttggtt
     7441 catctagtat atagaaattc tttccattag agattttgtg caattcactg gcaagtttca
     7501 tccgttgtgc ctcaccaccg gacaatgtcg ttgctggttg ccccatcgtc acataaccta
     7561 agccaacatc aacaatcgtt tgcagtttgc gatgaatctt tggaatgtgc ttgaagaatt
     7621 ctacagcatc ctctactgtc atttccaaaa tatcagcaat gctttttcct ttgtaatgca
     7681 cttctaatgt ttcagaatta tatcgtttac catgacatac ttcacaagga acatagacat
     7741 caggcaaaaa gtgcatttcg atcttaataa ttccatcccc gcgacaagct tcacaacgac
     7801 cgccttttac gttgaaacta aaacgccctt tcttataacc ccgcattttt gcctcgttcg
     7861 tttgagcaaa taacccacgg atatcatcaa atacacttgt ataagtcgct ggattacttc
     7921 tcggcgtccg gccgattggg ctttgatcga tatcgatgat cttttcgata ctttcgtagc
     7981 cggaaattgt cttgaattta cctggcttag cagaattctt atttaatttt tgcgctaacg
     8041 atttctttaa gatactattg accaatgtac tctttccaga accagacaca cctgtgactg
     8101 ctacaaattc tcctaatgga aattcaacac taatattttt cagattgttt tctgaagcac
     8161 cagtgatctt gattgctttc ccattacctt tacggcgttc ttttggtaca gggattgatt
     8221 ttttccctga aagatatttc cctgtcaaag aattttcatc attagctact tcttcaggag
     8281 tgcctgcagc cacgatttgt ccgccttgtt ctccggcccc aggtccgacg tcaatcaaat
     8341 aatctgctgc ccacatcgta tcttcatcat gttcgactac aacgagggtg ttgccaagat
     8401 cacgcatttt ctttaatgag ccaatcagtc gatcgttgtc gcgttgatgc aatccgatcg
     8461 atggttcatc taaaatatac aaaactccag aaagattcga tccaatttgt gtagccaatc
     8521 tgattcgctg cgcttctccc ccagataatg ttccggaagc gcgactcaaa gttagatagt
     8581 ctagtcccac attttccaag aatgaaagtc tgtcatttac ttcttttaaa atcggtttgg
     8641 caattacttt ctcttgttca gataacgtta agtttgagaa gaaatctact gctttttgaa
     8701 tagacaactc atttgtttgc ccgatattcg ttccattgat ttgaacagat agagcttggg
     8761 gattcaatcg ataaccttta catgtctgac aagttaattc tgtcatataa agacgcattt
     8821 gctcacgcgt gaaatcactg ttcgtatcat gataacgacg ctcgatattc ttcaataccc
     8881 cttcgaaagt cgtttctaca tcgcgaacgc caccaaaatc attttcataa tggaaatgga
     8941 aaagatcatc tgtcccattc aagatgatct cctgatgctc ttccggtaat tcttcgaatg
     9001 gtgtatctaa atcgataccg aaactttcac aagcctgcgc taacatttga ggataatact
     9061 gagagctgat tggattccat ggaacgatag caccttgact aagtgtcttc gttctatctg
     9121 gaatcactaa atcttgatcg acttctaatt tgacacctaa accatcacag tctggacaag
     9181 ctccaaaagg cgcattgaat gagaacagtc ttggttctag ttcgccaaca gtaaatccgc
     9241 aataaggaca ggcatagtgt tcgctaaaca acatttcttc ttcaccgatc acgtcaacca
     9301 gagcatagcc gtctgctaat cgcagggccg cctctaaaga atcaaatagg cgagaacgaa
     9361 tcccttcttt cacaatgatc cggtcgatga cgatcgcgat atcatgtttt ttgtttttct
     9421 ccagctcagg cacttcactt aaatcataga tctcaccatc gactcgaacg cggacgtatc
     9481 cctctttttg gattcgttcg aatatttttt tatgttgtcc ttttttcttt accacgatcg
     9541 gtgccaacac ttggatcttt gtgcgctcag gcaaagccat tacttgatct gccatttgat
     9601 ctactgattg actggtaatc tcgatatgat cgttgggaca aatcggatgt cctacacgtg
     9661 caaacaacaa gcgtaaatag tcattgattt ccgtcacagt acctactgtc gaccgaggat
     9721 ttttacttgt tgttttctga tcaatagaaa tcgccggact caatccgtcg atgctatcta
     9781 cgtccggctt atccatttgt cctaaaaact gacgagcata agcagaaagg ctctctacgt
     9841 atctccgctg cccttcagcg tacagcgtat caaatgctag ggaacttttt ccagaaccgg
     9901 acaaacctgt aacgaccacg aatttatctc gtggaatcgt tacatctata tttttcaagt
     9961 tatgggcacg tgcaccatga atcgtaattt tatcgttagc catgttgtcc tcctactttg
    10021 aagcttttaa ttctaaaatc atatctcgca cattcgcggc tgtttcaaaa tccaaagctt
    10081 ttgccgcatc tttcatttct ttttctaatt tcatcaataa cgtgtctttt tcttccttcg
    10141 tcatttcttc gtacgagact tgtaccgctt cttttgtatc atcttccgat tctttcgtaa
    10201 tagaaatcaa atctcggatt tctttaatga tcgtttttgg aacgatccca tgttcttcat
    10261 tgtacttctg ctgaatcgta cgacgtcttg acgtttcatc catcgctaga cgcatagaat
    10321 ctgttacttt gtctgcgtac atgattactt taccttcttc attccgagca gctcgtccaa
    10381 tcgtttggac taaggaacgt tcactacgta aaaagccttc tttatccgca tccaagatag
    10441 caacaaggga aacttcaggt acgtctaatc cttcacgaag caaattgatt ccaactaaca
    10501 catcaaattc acctaatcga agatctcgaa tgatttctgt tcgttccagc gttttgatat
    10561 cactatgcaa atatttgact ttgatcccta attctttgaa ataatctgtg aggtcttctg
    10621 ccattttttt cgtcaatgtc gttacaaaca cccgctggtc tttttcgact cgttcattta
    10681 tttcgcctac tagatcatcg atttgtccca tgattggacg aacttcgatc actgggtcaa
    10741 gcagccctgt cggacggatg atttgttgga taacggtatc tgtttgttca tgttcatatg
    10801 gtcctggcgt cgcagagaca taaatgatct gattgacatg tttttcaaac tcttctaacc
    10861 gaagtggccg attgtctaaa gcagatggca aacggaaacc gtaatctact agcatctgtt
    10921 ttctcgctcg gtcaccatta tacattcctc tgatttgagg catggttacg tgcgattcgt
    10981 ccgccacgat caaaaaatct tcaggaaaga aatccaatag cgtataaggc ggttcacctt
    11041 ctttacgtcc gtccatatgc cgagaataat tttcaattcc cgacgtgtag cccatttcta
    11101 acatcatttc aatatcgtaa ttggttcgct gttctaaacg ttgagcttcc agaagtttgt
    11161 tttcatttcg caagacagtt aaacgctgtt ctaattccgc tttgatattc gctacggcat
    11221 gctccatatg ttcatcattc gtcacaaagt gagtcgccgg gaagattgct acgtgttccg
    11281 tttctcctaa aacttcacct gtcagtgcat tgacttctcg gatacgttcg acctcatctc
    11341 caaaaaactc cacacgtaag gcacgttcat ctcttgaagc cggaaaaatc tccacgacat
    11401 caccgcgcac tcgaaaacga ccgcgctgaa aatctatatc atttctttcg aattggatac
    11461 tgactaagtc acgaatcaac tgattgcgat ctagttctgc tccctgtcga atcgaaacta
    11521 cttgtttttg gtactcaaaa ggcgagccta atccaaaaat acatgaaacg gatgcaatga
    11581 cgatcacatc atttctttct aataatgaac tagtagccga atgtcgtaat ttgtcgatct
    11641 catcatttac acttgaatct ttttcgatat acgtatcgct tgaaggcaca taagcttcag
    11701 gctgataata atcataataa gaaacaaaat attcaacagc attgttaggg aaaaattcct
    11761 tgaattcccc gtacagctga ccagccaatg ttttgttatg ggcaataatc agtgttggtt
    11821 tattcacttt ctcaataaga ttggaaatcg tatatgtttt ccctgttcca gtcgcaccta
    11881 ataagatttg tgcttttttc ccgccgacta cgccatccac taattggtta atcgcttcag
    11941 gctgatcacc agccggttgg tatttcgaga caagctcaaa gtgtcgggag gtatctctct
    12001 caatcattct tcgtcctcct tttcattcat tccttttcat tttacgacca ataaaaagaa
    12061 aaggaaagca aaggcattcc ttactaaaaa attataccac accgaacata ctttcgccac
    12121 ttttttatct tattatacat gttatgtttt ataacataaa atgttagaaa ctcgcatttt
    12181 tttcattttt tcgagcttac agtcttgtct gattcagcac ttttcgccta taataataaa
    12241 atattggaaa tttaggaggt tgattatgaa aagaaagcat ttatttcact catttgtttt
    12301 cttaatgaca ggacttgtga catttgcgtt tggtcatgtt gcacaagctg acaacactac
    12361 aacggcagag tcaagcacaa gcgagacaat gcaaaaaata gagcccaaaa aagacgtata
    12421 cgtaattgcc agtgactctg cttttgctcc atttgaattt caaaacgcaa agggcgagta
    12481 cgaaggcatc gatgtaaaat tgatggaaaa catcgccaaa ctgcaaggat ttaacatccg
    12541 aatggactac cgtggtttct ctgctgcttt gcaagctttg gaatcaggac aagcagacgg
    12601 aatgattgcc ggaatgactg taacagatga acgaaagaaa agctatgatt tttctgattc
    12661 ttactttgat agcgggatcc aattagctgt caaaaaagga gatacttcga tcaagtctta
    12721 tgaagatttg aaaggaaaaa cagtcggagc aaaagttggt accgaaagtg ctgaattttt
    12781 aagcgaccat gaatctgaat atggctattc tatcaaatat tttgatgctg ctgatcaatt
    12841 gtacgacggg ttaaaagttg gcgcaatcga tgcaatgatg gatgattatc cggtaatcgg
    12901 ttatgcgatc aaacaaggac aagaattgga aacaccgatc aaaaaagaat ctggcggaaa
    12961 atatgcattt gccgttaaaa aaggacaaaa tcctgagctt cttaatatgt tcaatgaagg
    13021 attgaaagaa ttaaaaagaa caggcgaata cgataagatt attaaagaat atgtagcgga
    13081 tggttcagaa acttccagtg agtcttccac tgctgatgaa tcaacatttc ttggacttat
    13141 ccaaaacaac tatcaagcat tgttgaaagg actttggaga acaattctcc taactttgat
    13201 ctcctttgct ttggctatag taatcggtgt gatttttgga ttattcagtg tagcaccaat
    13261 acgcgctctt cgcacaattg ctagtattta tgtcgatatc atccgaggaa ttccaatgat
    13321 ggttcttgcg ttctttattt tctttgggtt gcctggtatc gttggattta cgattcctga
    13381 cttccttgct ggtgttatca ccttgactct taacgcaagt gcttatatcg cagagattgt
    13441 tcgaggcggg atcaacgccg ttcctgtcgg acaaatggaa gcttccagaa gtttaggtct
    13501 ttcttataat cggacaatgc aaaaaatcat tttgccgcaa gcgatacgca tcatgatccc
    13561 atcattcgtc aatcagttcg ttatctcatt aaaagacaca acgatcattt ccgcaatcgg
    13621 tgtagtcgaa ttgctgcaaa ctggtaagat cattgttgca cgtacaacac aaagtacgta
    13681 tgtttacttg atcattgcga tcatgtactt gattttgatc acagcattga caaaattagc
    13741 aaaagtttta gaaaagaagg tgaagtaaga tggcagaaaa aatcttagtt gagcatttag
    13801 tcaaaaaata cggcgataac acagtattaa acgacatcaa tgtatccatc aacgaaggag
    13861 atgtcgtctg tatcattggc ccttcaggtt caggaaaaag tacattcttg cgttgcttga
    13921 atcgtttaga agaaccttct gacggtgaaa ttattattga cggcgcccac ttgatggata
    13981 aaagcacaga tatcaacttg gtgagacaac atatcggaat ggtattccaa cactttaacc
    14041 tattccctca tctttctatt ttggaaaaca tcattttagc accgatggac ctgaagaaag
    14101 aatctagaga agaagcagag aaaaaagcaa tcaaattact gcagacagtt ggtctagaag
    14161 ataaaaaaga tatgtatcct gaaatgcttt ctggtggaca aaaacagcgt gtggctattg
    14221 ctcgtgctct agcgatgaac cctgacatca tgctttttga tgagcctact tctgcactcg
    14281 atcctgaaat ggtcggtgat gtcttgaacg tcatgaaaaa gttagcagaa caaggtatga
    14341 cgatggttat cgttacgcac gaaatggggt tcgctaaaga agtggccaat cgcgtcatgt
    14401 tcattgacga cggaaacttt ttagaagatg gaacgcctca acaaatcttt gaaaatccgc
    14461 aaaatgaacg gacaaaagat ttcttgaata aagtattaaa tatttaataa aaaccaaaaa
    14521 tgcacggtcg ccatgaaggg accgtgtatt tttatcttct tattttctca cctaatcgta
    14581 tcagccgcat cgcttgtttt cctgcacaaa caagcaatcg ctctacctgt tccatcgcat
    14641 aagaaagtac catcggttca ttgatgatat caagtacata gtcgatacca ccctcactta
    14701 attgagcttg atttttcttc tccatattat gtagttgtgg gacaactttc tgacaatcag
    14761 caattgtgtc aagaacggat caatccttta ttggagttgc tcgaaagggt cttctcttat
    14821 tacggacagg ctttgctcac tgtacataga caacaaatca ttatcctagt caatcggatt
    14881 tccagagcct cattgctatc attgcttgat aaaatccaga caaagtttaa taaaatgtat
    14941 cagttacagc taaactttgg aattgggtcg ctttgttata cagagcaaga gacaccgcaa
    15001 tctttccttc atgccaaaca agtttgcgaa tggatagctt tccatcaatc tgtgaacgag
    15061 atccgctttt ttgaagattt agacctcggg atcgttttac cagcaatccc gtcagatcaa
    15121 cgaacattat atgtaaaacg tattttgaaa agcctgacag aagaagaagt acatttgttc
    15181 aagaagactt tggcatgctt cagtaaaaat aacggaagca tcaaaaattg ctcagaagaa
    15241 ttatttattc ataaaaacac cttgcaatat cgattgaaca agttccattc tctcactggt
    15301 tataccccta gaaactatga cgattatcat attctaaagc tggctttttt gttggtacaa
    15361 acatgacaac tacaaaaaaa ccaggcgaac atatgattgt tcgtctggtt tttaattatt
    15421 aatgacttag cgtttttact agaaaaacta cttccgcttt ttcccgccaa aagcatcttt
    15481 aaacttatcg aagaaaccgt cttcttgctg ttcttcaact ttgatgccgc cagcttcagc
    15541 gaatgcacgt aatgcttctc tttgttgttc atttagattt ttcggtgtga tcagttttac
    15601 tttgacatgt tggtcgcctg ttgcaccacc acgtaacttc ggagcgcctt ttccacgtaa
    15661 acggaagttc gtacctgtct gtgttccagc agggatcttc aatttcacat ctccatggac
    15721 agttggtact tggacttcat cccccaaagc tgcttgtaca aaacttaaag gcaattcata
    15781 gtaaatttca gaaccttcac gatcaaagat atcactttct tctacacgga agaccacata
    15841 caaatcacca aatggtccgc cgttttctcc agcttctcct tgaccagcta aacgcatttg
    15901 ttgtccttct tctacaccag ctggtacatt cactttaact gaatgtgctt tcttctcatg
    15961 tcctgagcca tgacaggttg gacaaggttc tttgatttct ttccctgtcc catgacaaac
    16021 atcacaagtt tgacggctca tcactcgtcc aagtggtgtt tggcgttcta cattaatcgt
    16081 acctgcacca tgacatttat gacaagtagt tggttgtgtg cctggttttg caccgttccc
    16141 accacaagta tgacagattt cttcccgatt atacttgatt tctttttcta caccaaaaat
    16201 cgcttcttca aaagacaagt cgaccgtata ttgcaaatca gcaccttgac gaggcgcatt
    16261 tggatcgact gaccgaccgc ctcctccaaa gaaagagtcg aagatatctt caaaaccacc
    16321 gaagccgcca ccagagaagc cgccgaatcc accaaatccg ccaccagctc caccagcacc
    16381 gtaatttgga tctgtgcctg catgcccgta ttggtcatat gcagcgcgtt tttgcgggtc
    16441 acttaaaatc tcatatgctt ctgatacttc tttgaatttt tcttccgcat caggttcttt
    16501 attgatgtca ggatggtatt ttttcgaaag cttacgataa gctttcttta tttcatcttc
    16561 tgatgctcct tttgagagcc ctaagacttc ataataatca cgttttgttg ccataggctt
    16621 tcctcctatt ggttctcatc attttcacgt cgcttgttat catatcatat tttgtttgaa
    16681 acaaaaaccg atacttcaaa ctatttttca aaagaaaaag ccaaagtagc aactttggcc
    16741 ttctctcgaa attcagtgaa ctgattattt atcgtcgcca tcgacttctt caaaatcagc
    16801 atcaacgaca tcatctgctc cgccttgagc tgcttctggg ttttcttgag cttgttgttg
    16861 tgccgcttgt tcgtatagtt taacagtcaa gttttgaacg atttcgttca atgcatcgcg
    16921 tttagttttc atttcttcga tgttgtttgc ttcaacagcg gctttcaatt catcgcgagc
    16981 tgtttctgct tttttcactt cttcttcgtc cactttgcct tctaattctt tcaatgtttt
    17041 gtccacagag aatagcaatg catctacatc attacgtagg tcaacttcct ctttacgagc
    17101 tttatctgct tctgcatttg cttctgcatc ttttaccata cgttcgattt cttcgtctgt
    17161 tagacctgaa gaagatttga tcgtgatttt ttgttctttt tgtgtaccta gatctttcgc
    17221 agatacattt acaatcccgt ttttatcaat atcgaatgtt acttcgattt gtggaatacc
    17281 acgaggagct gctggaatat ctgtcaattg gaatcttcct agagttttgt tgtcagcagc
    17341 cattgggcgt tcaccttgta atacatggat gtctactgct ggttgattat ctgccgcagt
    17401 tgagaatact tgtgatttac ttgttggaat cgttgtattg cggtcaatca atttagtgaa
    17461 cactccaccc attgtttcga tacctaatga caatggtgta acgtcaagta aaacaacatc
    17521 tttcacatca ccagtgatca cgccaccttg gatagcagca cccatagcaa ctacttcatc
    17581 agggttaact gatttgtttg gttcttttcc tgtttcttta cgtactgctt ctacaactgc
    17641 agggatacga gtagatccac cgactaaaat aacttcgtcg atttctgatt gtgataaacc
    17701 tgcatctttt agcgcttgac gtactggtac ttttgtacgt tctactaagt cagctgttaa
    17761 ttcatcaaat tttgcacgtg tcaatgtcat ttccaaatgc aatggtccag attctcctgc
    17821 tgtgataaat ggcaagctga tttgtgtgct tgatacgcca gataaatctt ttttcgcttt
    17881 ttcagcagcg tctttcaagc gttgtaaagc cattttgtct tgagacaagt caatgctgtt
    17941 ttcttttttg aattctgcta ccatgtagtc gatgattttg ttatcaaagt cgtccccacc
    18001 tagatggttg tcgccagctg ttgacaatac atcaaagaca ccgtctccta gttcaaggat
    18061 agatacgtca aaagtaccgc caccaaggtc gaatactaag attttttcgt cgcgatctgt
    18121 tttatctaaa ccataggcaa gagcagctgc agttggttca ttgacgatac gttctacttc
    18181 tagaccagcg atcttaccag catcttttgt tgcttgacgt tgtgcatcat taaagtaagc
    18241 tggcacagtg ataactgctt tttcgacttt ttcacctaaa tagtcttctg cgaatccttt
    18301 gatatattga aggatcattg ctgagatttc ttgtggtgta tatgatttac cttctacatc
    18361 tactttgtaa cctgcttcac cgatatggcg tttgattgat gaaatcgtat ttgggtttgt
    18421 cacagcttga cgttttgcaa cttcccctac ttggatttct ccatttttga atgatacaac
    18481 agaaggtgtt gtacggtttc cttctgggtt agcgatgatt tttgcttcgt ttccttccaa
    18541 tactgctact gcagagttag tcgtacctaa gtcaataccg ataattttac tcataattaa
    18601 cgtctccttc tttaattgaa agtgtaagtt ttatatccgc acaattattg tgcaacaatg
    18661 accatggttg gtcttaacac gcgatcatgt aatttgtagc cttcttgtag tacttctaca
    18721 atcgtatcgg ctggtgtatc ttcagtcgct ggaacagttt gtactgcttg atgaagatta
    18781 ggatcaaagg cttctccttt agcagggatt ttttcaattc cttcttcttc cagtgcgtta
    18841 cgcaaacttt ccaagaccat ttccacgcct tttttcaaac ctgccccttg atcatctgac
    18901 acttctgtgg ctaatgcacg ttccaggttg tcaattgatg gaagtaattt tttggctaaa
    18961 tcttgtgaac ggtatttttg taattgctca cgctcatttt ttcctcgatt tgccatattt
    19021 gcgatttcag cacgagcccg caagtattta tcttccattt cttctaactc agcttttaat
    19081 ttcgctgttt cgaattcttc cgcttcagct tctgaaatac ccgaatcttc tgcagcttcg
    19141 acatcgattt ccggttctgg ctgagcatct gtcatttctt tatctagttc ttcttgattt
    19201 tctttcacga tacagtcttc ctttcattat tcagtcagct gtttgattgc tcttactaag
    19261 aaagagagaa cgatcagctg caaatagctt cgttattcac attacgagtt tgacaagtca
    19321 agcgaacggt agtaatcagc aagtttagct gctagttctc ttcgataaac atcgactaga
    19381 ccgaacatcc gagaataagg catactagta ggccctaaca aggctattgt acctcttcca
    19441 tgaccatcta tctcgtaact tgcttgaatc atactcatat tctgcaataa atcattgcct
    19501 aattcagcac caatcttgat atgaatattg ctatccatcg gagtgatcat ttgtgtaagt
    19561 ccgtctgagt ctttcataaa agaaaacatc gatttgaact gatcaagatc ttgacggggc
    19621 tcataatcca agagattcat ttgtccactg acataaacct tatcttcaaa aacatgacca
    19681 agaacggtgt caaataactg agacatccct tctgtcgttt ggaaatattt atgaagaatc
    19741 atcggaatct ccgtacgaag tttatggtac accgtcatca gaggatctcc gactagacgg
    19801 tcgtttacaa tctgtaccat tttttctagt tcctgacttc ccatatattc gggaagagtg
    19861 aacacttggc tttctacatt ccccatatct gttacgacga tagcaataat ctgtcgatta
    19921 ttcaagggta cgatacgaaa gccagtcagt cggcggtctt ttatttcagg tcccaatgaa
    19981 aaagtcgtat agcttgtcag ctcagataat atctcagcag attgcttaat aatctcattg
    20041 atttcatgaa attctctgcc tagagactga cgaatcagca gcaaatcatc ttctgctacg
    20101 cgagcaggtt ttaacaaatg atccacgtaa taacgatagc ctaaagcaga tggaatccgc
    20161 ccagaagaag agtgagtctt caacagcaat ccttcatctt ctaatgcttt catatcattg
    20221 cgaatcgttg ctgagctggc cttgactccc tcagacatca atgttttgga accaacgggc
    20281 acgccactac tcgtatagtg ttggatgatc agacgtaaaa tgtcgttttg tcgctttgtt
    20341 aacatcttcc tcacctctca ttagcactca tatattctaa gtgctaatcc acttataaat
    20401 atagcaagaa gcccttctcc tgtcaagaga aagactccat tttttagcac tctaaacaca
    20461 agagtgctaa ttctaaataa ttgatgtaaa tccagttata
//
LOCUS       DAZUKZ010000042        19101 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000042 DAZUKZ010000000
VERSION     DAZUKZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.042"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(116..1684)
                     /locus_tag="ACG8EF_001470"
     CDS             complement(116..1684)
                     /locus_tag="ACG8EF_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFS4946783.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1688..1969)
                     /locus_tag="ACG8EF_001471"
     CDS             complement(1688..1969)
                     /locus_tag="ACG8EF_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS4946784.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(1966..2889)
                     /locus_tag="ACG8EF_001472"
     CDS             complement(1966..2889)
                     /locus_tag="ACG8EF_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFS4946785.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2886..3764)
                     /locus_tag="ACG8EF_001473"
     CDS             complement(2886..3764)
                     /locus_tag="ACG8EF_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFS4946786.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3751..5088)
                     /locus_tag="ACG8EF_001474"
     CDS             complement(3751..5088)
                     /locus_tag="ACG8EF_001474"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFS4946787.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5091..6194)
                     /locus_tag="ACG8EF_001475"
     CDS             complement(5091..6194)
                     /locus_tag="ACG8EF_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4946788.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6268..7776)
                     /locus_tag="ACG8EF_001476"
     CDS             complement(6268..7776)
                     /locus_tag="ACG8EF_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4946789.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7742..8434)
                     /locus_tag="ACG8EF_001477"
     CDS             complement(7742..8434)
                     /locus_tag="ACG8EF_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS4946790.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8456..9430)
                     /locus_tag="ACG8EF_001478"
     CDS             complement(8456..9430)
                     /locus_tag="ACG8EF_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4946791.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9730..10602
                     /locus_tag="ACG8EF_001479"
     CDS             9730..10602
                     /locus_tag="ACG8EF_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4946792.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11184..13382)
                     /locus_tag="ACG8EF_001480"
     CDS             complement(11184..13382)
                     /locus_tag="ACG8EF_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4946793.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13614..14606
                     /locus_tag="ACG8EF_001481"
     CDS             13614..14606
                     /locus_tag="ACG8EF_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4946794.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14666..16087
                     /locus_tag="ACG8EF_001482"
     CDS             14666..16087
                     /locus_tag="ACG8EF_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFS4946795.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16080..17579
                     /locus_tag="ACG8EF_001483"
     CDS             16080..17579
                     /locus_tag="ACG8EF_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFS4946796.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17618..19012
                     /gene="uxaC"
                     /locus_tag="ACG8EF_001484"
     CDS             17618..19012
                     /gene="uxaC"
                     /locus_tag="ACG8EF_001484"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS4946797.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         5749 a         3887 c         3370 g         6095 t
ORIGIN      
        1 taaaaacatt gatgcataag attgataact ttttttacat aacaatatat acaaacaaaa
       61 aaattcacta actagtgaaa ataagttagt gaacctcatt tattaaagaa ttaaattaca
      121 gatgatcttt tccatcatac ttgaaaaact caaaatcagc ataacttcct ctttttttat
      181 ttagatcatg cactgctatc ccgataaaat ttccagtaaa ccctcctgag agtatacaca
      241 tgtttttttg cggacctact ttcttccatt cttcagttga tacgtctttc aaatgaaact
      301 gtgctgtctc tttctttaca gtgactttca aaaaagtctt cttttctgtg agtggaatcg
      361 tccaatcttc tatttgtgct actccgtttt ctgattctat catacgtagg cattttccct
      421 tgtcctcatt ccaagtaaca taacaataaa tataattgga ttcatttaag tacagtacca
      481 gtccagccat ctgattaaaa ttattaggca tgtattcaaa tgctgtctcc gcatcaaaag
      541 taaaatccgt ttgccgaata gctaacaaat gatgttcgaa ggtagattga agagattcac
      601 cggaaatcag acgtaaaaaa cctggtctac attttgtatc acaccaatca gcatttggca
      661 aaatgcgtct ggcattccag ttcttcttta gctctccttc aaatgtatcc ataaactgat
      721 gatctttttc ttgttgtacc tcttcatttg tctcaatcat tatttcttcc atcggagttg
      781 tctggctatc tttaagtctt aaccaatcgt cggacgtcca atacacttct tgaatagcgg
      841 tttctcttcc caaaatagcc gcaccgttta atggtctggt acaaagatat gtcatgtacc
      901 atcttcctga cggggtttgt actaagctcc catgtcctgt acattgcaat gtagaagcag
      961 gtttgtcact agctgtcagc ataggaaaac caggatcaag ctcgtaagga ccagtaatat
     1021 tctttgctcg gcaaactgta actgaatgtc ctcttcctgt accgccttct gccgtcatca
     1081 aataataata cccgttacga aaatacaaat gtggtgcctc cgttttcgct aattctgtac
     1141 ctgcaaatat tttatagatg ggaccatcaa gcttcttctt ctctgcatca tattcttgta
     1201 atacgatacc gctggattta ttcggtgtgt ccagacgata atcccatagt tcattgacaa
     1261 gccatttttt tcccgtttca agatcatgga acaaagaagg atcaaagccg ttactattta
     1321 aataaattgg ttcggaccaa ggacctgtga tctcagatgc agtgatcaaa taattgtgga
     1381 catctttaaa cggacggcct gcacttttga catctgtgta aacaagataa aacaatccat
     1441 cagcataact gatctgtggc gcccatatac ttccatttgt aggatttcct tgcaaagata
     1501 cttgagtggt caaaatatcc gttgtatgtg accagttgac taaatttttc gactcatata
     1561 ctcggattcc tggaagccat tcaaaagagg agacaataat gtaataccgc tctttcactc
     1621 ttaatatatt aggatctgga ttgaatcctc ttaaaatagg atttttcact ttgatagtct
     1681 gcatgattca ggctcctttt ctcattctta ctcgatataa actgaatgcc ccagctgcta
     1741 aaaaagcaag tgaaataaac aacacaacgg caaacagacc agtcatgtcc tgtaataatc
     1801 caaatgtatt ttctaaagac aaagttgaat gatctacagc aagcgactga taactttttt
     1861 taaactctga cgcagacata ttacttaaag taactgctaa cccgccaaag aaaatcaaag
     1921 atagactgcc accgaccatt cctagcaata gttcccattt tcttttcatt tctgaacccc
     1981 ctgcttttct aaatgtatcc attcaggtct tttgatcgat gtttcataat tgttattacg
     2041 ttcagagaat ttgacagtct gttcattttt cttgtcatcc caattatccc aaccagttgg
     2101 gtgaacatgc ccacctatct tacacgcttc aaataagaca tttgcatacg gacgccacgg
     2161 tcttcctaaa tagacgtttc tagtatcagg ttctgcagtc aaggtacagc tttcaaaacg
     2221 ataccctgtt ttttgatttt tcggcgtaga tgctgctgtg atgtatccag gatttttact
     2281 ggctaagcta tggatatgac actgatgaaa aacggcatct gccccaccaa atatgaaatc
     2341 caccgttcct tcaatccagc aatattcata ggtctgacga caatactcag gattggcaga
     2401 agccggtgta caaaaaggag taccatcttt ttgcaaatca ggcaacggac ccgtgcataa
     2461 ggtatcctga taaccagata gtcgacagtt ttttaaagat acgcgatctc ctgaattgaa
     2521 taaagcaaca gcctgtccta ccttttctcc tggacctgct gtattttcga tgtgaagatt
     2581 ttctaatacg acattcgatc cttctaagaa aaaagtagca cttcggaaag tccctaactg
     2641 gctaccgtca agatactcct gttttgcaaa acgattaccg gagataacta ctgttcctgc
     2701 tcccacaagt ttaaaattgg ataatctgct ttctatatat tcatcatatc ttccttcttt
     2761 tactatcaag acctttggat ggctatccgg caggcctgcc aaataatcga ttccttcttg
     2821 gatagtttca aaatccgtcg gggtatctat agcattcttt ccaattatca gtttgtcttt
     2881 catcctcata aatgctccct tatgaattgt ttccaaatat ttctcatttc ttgtgtttcc
     2941 atatgaccgc ctgttaaaga aaaagaagaa aagttttctg gactacccat tgattgatac
     3001 tttttgtcca gcccttttct cagcttcagt acgccttcgt ccaagcacat cgtatcgttt
     3061 tttccaacta agctaagacg agggcgcgga gcgatttttt cttggattgc taacgttgta
     3121 taagctgtca atagatttgg cacatagtaa taaaacccat gatgatctag gcggcgatgt
     3181 agcaaaagtg tttcgatgtc cacctgtgcc gctagatcaa tacaaacttt gacacgagaa
     3241 tccaatgctg tcacccacca gcttaataag cctcccatag aaagaccaat cgttgcgagc
     3301 cgttgagtat ctgtgtcttc ccttgtctcc aaataatcga tcaagctgat cacatcataa
     3361 atacgcattc cccataacgt ttttcctgtt aataaaaatt gtttgaacaa ctcgctttca
     3421 gaaatacctc cgcgttcttc aaatccccaa gcatcaatcg accaaacagc atatcccatt
     3481 cctgtcagta cttctgcaaa agatggagaa gctaagtaag gagcaccatg gatcaattca
     3541 cttttcccga ctgaaaaatc tccgccgtga gaatgcaggt aaaggaccgt cggaaacggt
     3601 ccttcttttt ctggataagc atagagggct ggtactgttt ccaattcatt taagtacaat
     3661 tgatagtatt caagcataaa accgttttgt ttttctcggt ataatcgttt tccactaggg
     3721 gtatgtctgc tagatggagc acctaaaaaa tcattcaact gcataagcaa agtttttacg
     3781 aacaaccagt tcatccatat tttctgattg atttgattgg aatttctcaa tactgatttc
     3841 tttagtattt tctaatagaa atgctggtcc ctcgtgattt tcaatcgaca tctggttaaa
     3901 ggaaacctta cccgcaaagc caatataaaa tccttgtctg ctcatttcct ctattcctgt
     3961 catcatagca ggaacaccgg gaactgcatc ttgggccatc gagacatcga tattgtcaaa
     4021 tgtgatttct tgtatgaatt gttccgctaa tccataaata aaacctgcag aagcatggac
     4081 attccttgcc gtgatattcg aaaaatggat ccttctgaat gctgatgtcc gttcatctat
     4141 cggataagcc tttttttccc atacatacgg ttctttccct cgcggaccac agaaataata
     4201 aagattcagt ataaatggac acatcacatt atccatgact atattggata ctcgaatatc
     4261 ttcaacgatc ccgcctcgac ctcgtctaga tttcaatcgt attcctcgat cagtttcttg
     4321 gaagatacaa tttgaaatgg taatattacg aatactacca ctcatttcac ttcctaatac
     4381 gacaccgcca tgtccatgga ccatcgtgca atttgtgatc gtgatatttt cgcaagcgat
     4441 cctttcgtac gtatcttcag tccctgcctt gattgcaata cagtcatctc ccacgtcaat
     4501 atggcaatta ctaatacgca catttttgca tgattctgga tcaattccgt ctgtatttgg
     4561 tgaatccgct gggttcaaga tcgtcaagtt atcaaacgtt gcattactgc aaagaatcgg
     4621 gttgatcgtc caacttggtg actggatcaa tttaatatct ttcacagtaa ttcgatgaca
     4681 gttatggaaa ctcatcaatt taggacgagg ataagcgaga ttatctggtt catttctaaa
     4741 agtatgccac catttttttc catttccatc taatgtaccg aaaccagtga cagagatgtt
     4801 ctctacattt tgagcataga tacaggaagc ataaactttt cgatggactc cttcccaacg
     4861 tgaatgaaca acaggataat cttttggatc gtcagagaat ttcagaaccg ctcctgcgct
     4921 taaatgaagt tcgacattgc tttttaaaaa taaagctcct gttagaaatt cacctgctgg
     4981 aacaactacg gtcccgcctc cgtcacttgc tgcagcatca atggcttgtt gtattgcttc
     5041 agtattcaat tcatcaattg atgcgccaaa tgtcaaaata tcatacatat ttataatgcc
     5101 tccacttcaa tcaatgcttg caagaatgca ccaacgcctt tttgatcatt acaaataatt
     5161 ggttcactga tgtagtaagc atatgaacca tctcgctgat ctgctccgcc taaccctgct
     5221 acttgacagt ttttattcaa gttcagccag ccttcttttg tttccagaac aaattcatca
     5281 agtaatcctt gatgtgattt cttcacttgt ggaagccatt cttctttgtt cagcagtccc
     5341 aaacgtattc ctttagccat tgcgtaaaca aacatactac tgccggaagc ttccagataa
     5401 tttcctttcc gttttgtttc tgttgttact tgataccatg tcccgctgtt ttcttcttga
     5461 tatggaagta atgcagccag tgtcttttct agaatttctt tcagcaaaga acaatctgtt
     5521 tcttcatgaa gtaattcata ggtatctgct gcagccatca aataccagcc aatcgatcgg
     5581 ctccaaaaat gatgggatag acctgttttt ggatcagccc atggttggac cttcttttca
     5641 tcccatgcat gaatcagcaa atttgttttt ggatcgaata gatgtttata actgatttca
     5701 aactgtctca aaacatcttg gtaatctttt tcttcactaa aattctggat aaattcagca
     5761 taaaaaggag aacccatata taagccatcc aaccaaattt gataaggata aatttcttta
     5821 tgccaaaaag ctccttcgga tgtgcgtgga tggtttgcca actgctggga aaggagttct
     5881 gctgcttttt tatatttttc ttcgcctgtt tctttgtaca ataaaaagaa aagctttcct
     5941 gtatttacat ggtcgatatt gtattcttca acgctgtagc cacggatcgt tccatcttct
     6001 tgcacgaaat agtcgatatt gtcttttatg aaatcaaaat aaattttttc ttgggttcgt
     6061 tcccaaagta attggaaccc tttaagtacg acgccatagt cataagacca ttttccttta
     6121 taccactttt cttcatataa gcgaggtgtt ctttccatga tactttttgc taatttcttt
     6181 gaccagtttt ccattatttg ctctccttta aaaattaaaa taggatttgg gacaaatacc
     6241 aagaagtatt tgtcccaatc gtctatatta ctgtgccgct gctttgtatg cttcctcgta
     6301 ctcttgtgtg attcgatcgc ctccttgtgc tttccagtct tctacagctt ttttgaatcc
     6361 atcttcgtca agctgtccca tgatgtattg caccgtagca tcaatgacga ttttttcaat
     6421 ttctgatcct acttcattgt tcgtcggtga atccaatggc acagaaggat ccattacagc
     6481 aaattcttca ttttctttga tcaattgatt agagaattct ttttcagggt ctgtatcttt
     6541 gaaagagaac gtcacttcac taggacgaga gcttgataac ggttgcacct cttgttgcca
     6601 aagatccata tcgataaatt cgatagaacc atcatcattc aattcataat gcgttccttc
     6661 gataccgcca gtcatcaatt tatacacatc ttcttctaaa agatcatcaa caaattgaag
     6721 taatttcttc aattgtgctt cgtcttttac ttgagatttg ggaaatgcta gcaagccgcc
     6781 aataccattt ccttctgacc aaacatgata accgccgcct tcaccatctg agatcttgtt
     6841 tacaggcact aattccatat catcttgaat accttgaccc atcgtcttca gattcttgat
     6901 atccaccatt ccagtgtaga ttccagttct tccttgtgca aattgttctt gctgatcagt
     6961 cttttgggta actgcaaagt cttgtgccaa atagccattc tcaaatagat ctcttgaaaa
     7021 attcaaagct ttcatatact cttctgtatc aaattctgga gtgaatttgc cattatcgtc
     7081 tacagcccag ccatttggtg ctccaaaata agaagtgaaa agtttaaagc tggtataacg
     7141 aatatcacta ggcgcacgat cgccaaaacc aactgtgtcg tccacaccat ttccatcagg
     7201 atcgttttct gtaaattgtt tagctacttc ataaagctca tccatggtag ttggaacatc
     7261 tagtcctaga ttatccaacc agtcttttcg aatgactaag ccagcacgag cgtagtcttt
     7321 ttgtatcggt acgccgtaca aggcaccgtc gatactcgca gcttcgattc tatcttcaga
     7381 aatttgactt aaatttttat agtcttttaa atattctgat acatcccaga atacgcctga
     7441 ttttaacgca ttgcggacag aagagttttt caacatcgca atttgaagtg taacgatatc
     7501 tcctagttca tcagaagcca atgcagttgt taatctttct tcttttgaag catcaggaat
     7561 ccagttgaat gtcagttccg tatcggtttt ttcttcgatc aagttcacaa tcgtgtctgt
     7621 aggcggtgaa gcatgatgca aaatatttaa ccaagtgatt ttagcttttc catcttctgt
     7681 ctctgaagaa ctgttacttg aactgctgcc gcatgcggca agtgctagaa ttcctgcaaa
     7741 actaagacct aattttacga gcgttgcttt tttcataaat tttctccttc actgttttaa
     7801 taatattttt gttttcttct gttttttctg tatatgagtt cttccattcc aaatggcaaa
     7861 atatagaatt tgctgtccac gtgactgcgg aaaagaagag actgccaccg ataaaggtta
     7921 aaacacctgg cattgtttgg atgatcatca gataaatccc taccatactt aatatcaaca
     7981 ctaatgagta atgaagatgt gagaatccga ataaaaatgc cattttcatt ttgaaaaaat
     8041 atcctttcca atcataatga gccatgattg ggaaaatata gagtgctgag agcacatata
     8101 tgaacgcagc aatatacaaa gcgatcctaa gataccaatt gtgtatgaaa gaaagatcca
     8161 caagtaaaac aatcccgatg gcgccatata tccagctcat cagcaaggtt tcacgatagt
     8221 tttctttaaa atatgtgaag tacgttttcg tcaaaggaaa cgtttctttt gagcgcagcc
     8281 attttctaat cactgcaacc atcgcatacg atgcaggacc gaaagtaaaa actcccaaac
     8341 ctgcgatgac taaaccgccc cacagaaaat tcaaataaac caactgtaaa atacgattta
     8401 gccttccgtt tattttatct aatgactgca gcatatcttt ccctctcttt ctatcttagt
     8461 aaacgccgtc ttctcctagc tttttggcaa tcttattagc taaaattacc ataaacaatc
     8521 ctacgacccc tttaaacaat cccattgctg tactgaaact taactgtcca ttcttcaatc
     8581 cagctgtata aatataggtg tcgaaaattt ctgcaacgct tcgattcaat gaattcaata
     8641 acaagtacat atgctcgaac cctaagtcta atgtctgacc gatttttaga atcagcaagg
     8701 taatgattac tggacgaatt gccggcaatg taacatgcca tgttttgcgt aagcgtcctg
     8761 ctccatccat ttctgcagct tcatacaact gtgtatcgac tgcagttatc gctgaaaggt
     8821 agatgatcgt agaccagccc aactctttcc agatcacttg tccgatatat actgtgcgtg
     8881 tccattcagg ggatgttaga aaactgattt tttcaaatcc cattgttgca aggaattcat
     8941 tcaatacccc tccgtcaaca ttcaaaaata cataagtaat cgaaacaatg attacccaag
     9001 acatgaagtg agggatgtaa atgatcgttt gaatcccgtt tttcaatcgt ttgttcgtta
     9061 cttcatttaa tagtaaagac aaaatgattg gcatcggaaa aaacacgaag atatttaatc
     9121 cgaacaagat caacgtattt cttaataatg taaagaatgt cggttctgta aacaaacgaa
     9181 tgaaatgttt gaaaccgacc caaggacttc ccattattcc taaaaatggt tgataatctt
     9241 gaaaagcaat aacgagtccc cccataggaa gatacctaaa aatcaagaaa taaaggactc
     9301 ccggcagaat catcacatat aaaaatttat tcgttttgat tcctgcaatt attttttttc
     9361 gtcttctgag cttttcttct tttttcgata gcgtcagttg aaatggttct tgtttcttta
     9421 cactttccat aagcccctcc ttagttttgt gaatgtttgc tgcttatgac ttcatgatag
     9481 ataactatct gaattacgta tataatcatt tttatcgtaa gtaaaaacgc ttacatagca
     9541 atgatttttg tgctatacaa aacgataaag tgtatttttt cacaaataaa ctacgcatag
     9601 caaaccttct atttttttct atctgacact tagcatagga aatgattata tacgtaatct
     9661 ttcaccgatg ataaattgaa catatagaaa cagaaacatt taatttattt tgaggaggag
     9721 cctatctata tgaaaaagaa agtctctata ggagaaagaa gttttacact tttcaacaat
     9781 acgtttctaa ttttgctagc tttgatctgt atcgttcctt ttttaaatat catcgctaca
     9841 tcttttgctt ctacacaaga agtcgtcgcg aaaaagttta tcctttttcc tactaccttt
     9901 tctttagacg cgtatcgcta cattctttct acgccgacca tttttagagc acttgctgtt
     9961 tcgattggtg ttacaggtgt cggaacgatc gtcagtatgt gtgcaacttc actgatggcc
    10021 tacggtcttt ccagaaagta tttgtttggt cgaggtttcg tcaactttct tgttgtattt
    10081 tctatgttgt tcagcggggg gatgattcca acgttcttag ttgttcgttc tctagggctg
    10141 gtcaattctt actgggccat gatcctgcct gtggctgtta atgcaatgaa tatgatcatt
    10201 atgagaaact ttttccaagc cttgccggat agtttggaag aatcagcaaa aatggatggt
    10261 tgcacagatt ttggtgtatt tttcaaaatt atgctgccat tagctttacc gtctattgct
    10321 actatctcgc ttttttatgc tgttacttac tggaacactt atatgactgc tattttgtat
    10381 atcaacgatt catctaaatg gccgatacag attcttttac gccaaatcgt cattgtatca
    10441 agtgggatgc aagcagaaag tagtgccgtt gatgtgattc caccagcaca aacagttaaa
    10501 atggctgtta tcgttattgc aacagtgcct atgctgattg tttatccttt tgttcagaaa
    10561 tattttgtca aaggagcact cgtaggttct gtcaaagggt gattatgtac tgaacgcaaa
    10621 atggtagttg tgtcagaagt ggtcagcttc aagaaataag aaggcattta cgaaaattgc
    10681 ttcttaaatt tttgtgaaat ttcggcttat ttttcgaaga atctactcct ggaacaccgt
    10741 gtatcagagg ttgtgaaaga gacgttcagc cttgagcgat gataaaaact ctcacaaaca
    10801 ttgcgacgag taacccacag gagcaataag aagcgatttt gaaaaatagc ttcccatatt
    10861 tcacaaaatt atgccttatt gtcgatagcc tgtctcttga acgccattta ttcgtaaaaa
    10921 agaactgtga caagactttt gtcacagttc tttttgcgtg cttagccttg aaatttaact
    10981 atccataaat acaaatacta gggttattct aacttctttt attgcttgaa tcagtccaca
    11041 agaaaaacag cctcttgatt aaacggcgtt tatcttgttg attctgcggc aataaaatca
    11101 gccagcagca aaacatgaag tgctgttttt tgctgtcctc ttttattgct tgaatcagat
    11161 cacaagaaaa acagcctctt gattcacaaa tatttttttc gatattctcc cggcgtaatc
    11221 gattccttct ttttaaagaa acgaataaaa ttttgcggat ttcgatattg caatctttct
    11281 gcaatctctt ttacggagag gttagtctct ttcaaccatt tttttgctac ctctaaacga
    11341 tgattttgaa caaaatcacc aaatttttct cctgtttcct ttttaaatac attacttaaa
    11401 tagttagggt tataatgcaa cctatcagcg atcacatcta aagacagatc acgatcatat
    11461 tctgtgtaga cgatatgcat aatattctcc gacaaatttt taaattcatg atcagtgatt
    11521 gcttgagagc attctgtgat tggcagaact aattgttcta aaacaagttt ttctagttct
    11581 ttgggatgat aattgttcag tgcttttaca taaatttgtt tcatgttatc aaagtttttt
    11641 gaatctactc ccaataactg tcctaattga acgagttcat tgatcaagcg gaccgaaact
    11701 acttcccgac tcaaaggatt tttattttgt gcaaacaatg catcaatcaa ttcatgtact
    11761 aaattcttca cttcctgatt tccagaacga atggcttcaa acaatcgatt ttgttcttct
    11821 atcggatatc taataagtgt tttttcatga gcagcaggaa cgatttcctg ataaaaaata
    11881 attgaacttg gaccagtatt tacacgataa tacagggctt cttttgcccg atcaaccgat
    11941 tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc ctatactgat agttaaattc
    12001 aagtattctt ttacacgttt ttgaatgagt cggcaatact ccatgacttt tttgcttgct
    12061 tctgcatcat ctttatccat tcgataaata gtggcttgca tttcttcatt caaaacgatc
    12121 ggcagcattc gattttcctt tggcacaatt tccgtgatca tgttgttgat cgctaaaagt
    12181 agcaaatcac gctccccacc gtgactatct tctaacagat cgatttgaac caaagctgta
    12241 taatagcagt cattttgaga actataacca aattgctgca accgctgatc tagttctcgc
    12301 ctttctactc gatttctaaa taaactaagt acaaatagcg tttccagctg tggcttttgg
    12361 gtaactaagt gtgcacttaa cgcttctttt tctcctatga tccgactgac tgattgagcg
    12421 accaaatcta gttctttccc actgaatcct tttgatttaa gattcagacg ttcctgtatt
    12481 tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg aaaaaagacc aatgagtcct
    12541 accagtgtta aagacactgc aactagcccg attctcagat tgcggatcat tgcgccaatt
    12601 gcactagagt caacttctaa tctgtaaatc caattattat aatctgattt cagtactata
    12661 tacgttttat ctttttctct tatcactttc agctgattgt aatctagatc cacgtccgtt
    12721 aatacagttg gttgctcttt attttttact gtctgagtag taaaaaggtt gccaacttcg
    12781 ttttcaatag aaagtatgtc ttctccactt tcaccaacaa cttgttctat tgtatgtttc
    12841 tctatcgtag caattcccaa tgcatatttt tctttttcaa acattggcaa agaaatcacc
    12901 atttcgattc ctttgttttt cttttgccaa aataaattat ttttgttatt tatgtacttc
    12961 tgttgatact tttcaacttc ctcttcggtc agttgactca acgatccatt gattactccc
    13021 catttaccag ccaaactgac taaatcataa ttattatcat ccataacaat cgtttctata
    13081 aaatttagtt ctttcctgat ttcagaataa gtggagtaat ctgtatatgt cagcggatgc
    13141 ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag aaaacgaata ttcaatcgtt
    13201 ctaattttcc attcaacgtt agttttaact tgcctgagca aattcttttt gtctgttaaa
    13261 taactcgatt cgatcgattc ataggtattc tggtaaatga ataaactaaa tcctatgaca
    13321 agtacggctc ctaataaaaa catcggtaaa aatattttat aaaatactgt acttttcttc
    13381 attttcttcc tctcctcctt ttaaaataaa aggctttcat ttttctgtaa aattgatact
    13441 catctaatta aatgagcatt ccccgctttc aagaatagca taattaaaaa taaaaatcca
    13501 agtaattttt aacgtgtgca atcttttcat atcaaagatc tattgactta tcgtagttat
    13561 cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa ggaaaatgat ggaatggtaa
    13621 cgatcaaaga tatcgctaaa gcagctggcg tttctcatac gacagtttct cgtgctttga
    13681 ataacagcag tttaataaaa aatgaaacaa aacaaaaaat acgacaacta gctaaggaaa
    13741 tgaattattc gccaaactat agtgcaaaag gtttagtgaa tcaaaaaaaa tatctaatcg
    13801 gactattttt ctcaagtttg cataaaggaa cttcatcaaa tttcctagca gattccattg
    13861 caggcatcca ttcagtatta gacacaaatt acagtttatc tgttgaaagt atcgatgttg
    13921 ttgatttaac agaaatcaac ctgcaacgct acgatggact gatcgttatg agtcaaagtg
    13981 atgatgatca gcctttcatc gactatctca aagaaaaaga atttcctttc gttgttgtca
    14041 atcgtcacat caatgatcca gcggttatca acgtcactgc cgatgatgcg gccggtgtta
    14101 tgcaggcagt agattacggt attggatgcg gacatacgaa gatcgcttat attggcggaa
    14161 agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt attgaaaagt ctaaaaaaag
    14221 cgagcatccc tgtcaatcta gcctattttt taacaggaga ttacagctta gaaagcggcc
    14281 tccaaaacat ggaataccta ttatcacttt ctgacctgcc tactctggtt ttttgcggca
    14341 acgacgacat agcgattggt gcattgagag ctgtacattt agctggcctc cgtgttcccc
    14401 agaacatttc tctgatcggg ttcgatgatt ctccagtcgt ctcctatctc aatccgccgc
    14461 ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga aggaacaaag cttttactag
    14521 atttgatcga aggcaggacg atcgaacaat ctaaaattga gctctccacc ttgttgatcc
    14581 ttcgtgagtc gatcaattac ttgtaaacta caagtaattt tatataaaaa tgttaacgtg
    14641 tgcataacac agaaaggaag tttatatgaa aaaactgaca aaagaattag tgaaaaaaaa
    14701 cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat tttatgcgtg gatttattga
    14761 ttggcagatt cagcagctga ataaccaaca attgtttaaa ggtaatgttg caatcattca
    14821 acctttagaa caaggtcttg gaaatatgat gaaccagcaa gataatttat atacggtcat
    14881 tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc gagattatca ccgtagtaga
    14941 acaaatgatc aatccttatg atgaatggga tgcttatcta tctctagcag aaaatgacga
    15001 cttggcctat atcgtctcca atacaacaga ggcaggaatc caattcgtgg agactgataa
    15061 tttaacagat actcccccac agagcttccc gggcaaactg acagcctttt tataccgacg
    15121 tttcaaatta aacaaagctg gctttaccat cattccttgc gaactgattg acagaaacgg
    15181 cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa tgggatttag aacctgcttt
    15241 tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt ttagttgatc gcatcgtccc
    15301 aggttatcct cgcgaagatg caggtgtttt caatcaacgg cataactatg aagatcaatt
    15361 gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa gggcctaaag aattggagaa
    15421 tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc acagatgata tgactcctta
    15481 tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca gcgatggtcc ctcttgcttt
    15541 gttggcggaa ttggaaactg tagaagaagt aatgaaagat cctttattct ccgcatatgt
    15601 tgatcagtta ttcaatctag aattgattcc tatgctttct ttacctaaag atgaattagc
    15661 catttatgct gaccaaatca aagaacgttt cttgaatcct tttgcccatc acaaattaga
    15721 agcgatttca ttaaatagtg tttcaaagtt cagcacccgt ctgcttccag tgttcaagaa
    15781 gtatatcgaa gaacaaaatc aggtaccacc gttaatcact gtttcactag ctgcgctact
    15841 tcttatgtat cgaggcgatc aagtaaagcc acatgatgat gaaaagacga taagcgaatt
    15901 tactgatgct tggtctgata atggaacagc tattcctcga ttattgcaaa acgctgcgct
    15961 ttggggagaa gatttgtctc aaataccgaa tgtgacagat acagttcaag aaatagctgc
    16021 aaagatcgat caaaaaggtg tccggtcagt attacaagaa atgaaaggaa cgatggtcca
    16081 tgcttgaaac cctgcaacca acaatgaaat taaatcgacg agacagtgtg gtcgtcgcat
    16141 taacccctat tccaaaacaa acacaactga cgattgatca gcaaacgatc accactcttg
    16201 aagatatccc gcaaggtcat aagattgcat tgggtgattt gaaagctgga gacaacgtga
    16261 tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt aactgccggt caatggctgc
    16321 acactcataa tgtaaaaacc aatctttctg gtgaattgga ttatcgatat gaaaaagatg
    16381 tccatccaag tcattacctt tttgagaacc gaacatttca aggttacttg cgaaaaaatg
    16441 gaaaagtagg gattcgtaat gatttgtttt tagtacctac cgttgggtgt gtgaatggca
    16501 ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc agatctggga cagtttgacc
    16561 atattacgat tttaaagcat ccttatggct gttcgcagct tggaaaagat catcaaaata
    16621 ctcgagaaat tttagcggac gcagtaaatc atcccaatgc cggaggagtt ttagtatttg
    16681 gattaggttg tgagaacaat acagttcctg aattcaaaaa aattttaggt gaatatgatg
    16741 aagaacgagt gaaatttcta gtggctcaag atgtttacga tgagatcgaa caaggtgtgg
    16801 ctctattaga agagctttta acagcggcgg aaaatgacca gcgcatatca gtcccgctct
    16861 ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg tctatcggga ataactgcta
    16921 atcctttact tggtgcattt tctgactact tgattgctca aggaggcagc acgatcttaa
    16981 cagaagtgcc agaaatgttt ggcgcagaac aagtattgat ggcacgagcg gaaaatgaag
    17041 aagtttttga ctcaatcgtt gacttgatca atgacttcaa acagtatttc ttgtcttacg
    17101 gggaaccagt ttacgaaaac ccatctcctg gtaataaagc aggtgggatc actacattag
    17161 aagataagtc tttaggctgc acgcaaaaat caggaagctc tcctgtcgtt gatgtgctac
    17221 aatatggcga aaaaatcaga aaaccagggc ttagtttatt gcaagctccc gggaatgatc
    17281 ttgtagctgc ctctgctctt gcttcatccg attgccagtt ggtcttgttt accacaggaa
    17341 gagggactcc gtttggctca tatgtcccaa cggtcaaggt atctacgaat acaactcttt
    17401 ttgaacgcaa aggtcattgg atggatttta atgcaggcgt cttattagaa caaccaatgg
    17461 aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt agctagtgga gaagaaacga
    17521 aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa aaacggagta acactttgat
    17581 tttcataaaa cagtataaaa agaaagaagg aagatttatg tttttatctg atgatttttt
    17641 attaaaagac gattgggcga aaaagcttta ccattcctac gcaaaaaaca tgcccattat
    17701 tgattatcac tgtcacttag atccaaaaga aatttacgaa aacaacaatt tcagcaatct
    17761 gacagaagct tggttaggag gcgatcatta caaatggcgc ttgatgcgag cttgcggcgt
    17821 gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa aaatttttag cttggtgtca
    17881 aaccgtacca aaaattatcg gaaatccgct ttacagttgg tcacatcttg aattgaagcg
    17941 tttcttcggt attgacttgt tgataaatga agaaaatgca gagaaaattt ggacacaagc
    18001 aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa atcgcaaaaa aatcgaatgt
    18061 agaagtgatt tgtacaacag atgatcccgc agacgacttg cactatcatc agttgcttgc
    18121 tcatgaagaa ccagatttga aggttctacc ttcattccgt ccagataaag cactcaatat
    18181 tgaaaaagaa ggatttaatg catgggtcac actactggaa gaagcagctg accaaacgat
    18241 caccacctat caagaactga tagaagtctt agggcagcgg atagaatact tccatcaaca
    18301 tggatgtcgt gtttccgacc acgctttaga catcttacgc tataaagaag cggatgaaac
    18361 agtattagaa gagatttttc aaaaagcacg agctaacaaa ctattatctt cagatgaaat
    18421 cgatgcttat cttacagaaa cactgataca tttgacccac ttctatcatt ctcataattg
    18481 gacgatgcag ctgcacatcc atgcttatcg taactgtaat acacaaatgt tggaaaaatt
    18541 agggcccgat accggctatg atggaatgaa tgatctttct ttgactattc ctctgcaaaa
    18601 attattgaac cgtgcagaag aaacggatca gttgccaaag acgatccttt attcattgaa
    18661 tccgaatgac tatcctgcga ttttagcttt gatgggctgt ttccaaaaag aacagaatgg
    18721 aaaactgcag ttaggttctg gatggtggta caacgatact cgagctggta tgcgcgatca
    18781 attgacacaa tttgctgatg gtagtgcttt ggggaatttt gtcggcatgc tgacagattc
    18841 aagaagtttc ctgtcctaca ctagacatga atatttccgt cgtgttcttt gtgaattttt
    18901 aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa caacttttag gaagtctagt
    18961 tcaagcgatt agttatacaa atgctaaaaa ttactttggc ttttttgcct aattgattaa
    19021 tagtaacagt aacgaatcaa aaacaggata gtgaaataac ggaatccgtt tatccacgtt
    19081 ttagagaaga agacggacca a
//
LOCUS       DAZUKZ010000043        18960 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000043 DAZUKZ010000000
VERSION     DAZUKZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.043"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(9..530)
                     /locus_tag="ACG8EF_001485"
     CDS             complement(9..530)
                     /locus_tag="ACG8EF_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFS4946798.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            729..4421
                     /gene="nifJ"
                     /locus_tag="ACG8EF_001486"
     CDS             729..4421
                     /gene="nifJ"
                     /locus_tag="ACG8EF_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFS4946799.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4610..5497
                     /gene="cdaA"
                     /locus_tag="ACG8EF_001487"
     CDS             4610..5497
                     /gene="cdaA"
                     /locus_tag="ACG8EF_001487"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFS4946800.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5494..6663
                     /locus_tag="ACG8EF_001488"
     CDS             5494..6663
                     /locus_tag="ACG8EF_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFS4946801.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6687..8042
                     /gene="glmM"
                     /locus_tag="ACG8EF_001489"
     CDS             6687..8042
                     /gene="glmM"
                     /locus_tag="ACG8EF_001489"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFS4946802.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="ACG8EF_001490"
     CDS             complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="ACG8EF_001490"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFS4946803.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10328..10618
                     /locus_tag="ACG8EF_001491"
     CDS             10328..10618
                     /locus_tag="ACG8EF_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946804.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10631..11656
                     /gene="sppA"
                     /locus_tag="ACG8EF_001492"
     CDS             10631..11656
                     /gene="sppA"
                     /locus_tag="ACG8EF_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS4946805.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11659..12318
                     /locus_tag="ACG8EF_001493"
     CDS             11659..12318
                     /locus_tag="ACG8EF_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS4946806.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12475..13407
                     /locus_tag="ACG8EF_001494"
     CDS             12475..13407
                     /locus_tag="ACG8EF_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946807.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13561..14850
                     /gene="tig"
                     /locus_tag="ACG8EF_001495"
     CDS             13561..14850
                     /gene="tig"
                     /locus_tag="ACG8EF_001495"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFS4946808.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15097..16347
                     /gene="clpX"
                     /locus_tag="ACG8EF_001496"
     CDS             15097..16347
                     /gene="clpX"
                     /locus_tag="ACG8EF_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFS4946809.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16430..17017
                     /gene="yihA"
                     /locus_tag="ACG8EF_001497"
     CDS             16430..17017
                     /gene="yihA"
                     /locus_tag="ACG8EF_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFS4946810.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(17091..17246)
                     /locus_tag="ACG8EF_001498"
     CDS             complement(17091..17246)
                     /locus_tag="ACG8EF_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFS4946811.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            17382..18710
                     /locus_tag="ACG8EF_001499"
     CDS             17382..18710
                     /locus_tag="ACG8EF_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946812.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            18721..18930
                     /locus_tag="ACG8EF_001500"
     CDS             18721..18930
                     /locus_tag="ACG8EF_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946813.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT         6215 a         3370 c         4081 g         5294 t
ORIGIN      
        1 ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca
       61 aagatttctt tacccaagat gacagttgga acaaatcgaa tatgtgcttg ctcagcttcg
      121 ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact
      181 gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt
      241 tgtttgatct ctttgatcgc tttttggcca tcgctgattg tgagataccg gtgcatcacg
      301 ttccctcgct gtaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc
      361 ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac
      421 ggacaagcta agttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg
      481 attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac
      541 ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt
      601 ctaaaaatta gatgaccaat tcaaatagat acatgtaaaa tatttttata aataaaagaa
      661 aaaaatggca tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat
      721 gatgaatcat gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt
      781 atgcatttac cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat
      841 tagtagatca atggtcagca gcaggaaaag aaaacgtatt cggtcaacca gtaaaagtag
      901 tagaaatgca atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg
      961 cactgacaac tacatatacc gcttcacaag ggctgctttt gatgataccg aatatgtaca
     1021 agattgctgg ggaactactg ccaagtgttt tccacgttgc tagtcgggca gtgacgacaa
     1081 atgcgttgaa tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg
     1141 ctatgttagc tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg
     1201 cttctattga aggagaagtc ccttttttga atttctttga tggattccgt acgagtcacg
     1261 agattcaaaa aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaggaga
     1321 aattagcttc atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga
     1381 accagaatcc agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat
     1441 tacctgaaat cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg
     1501 acttggtgac ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag
     1561 ttgcacagac gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt
     1621 ttttgaatat ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc
     1681 atacagtaaa aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac
     1741 ctctcttatt agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg
     1801 gtggtcgcta cggattgggg tctaaagatg tgactcccga ccaaatctca gcagtttttg
     1861 atgaattgaa aaaagaccca agcatacgca aaaaacggtt taccataggg atcgtggatg
     1921 atgtgactta tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat
     1981 tccaggccaa gttttgggga tttggatctg acggaacagt tggagcaaat aaatcagcta
     2041 tcaagattat tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga
     2101 aaaaatctgg cggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttcag
     2161 cgtatcttgt cgagcatgcg gaccttgttg cctgtcacac accagcttac ctgcatagct
     2221 atgacttggt caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg
     2281 acgaacaatt agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca
     2341 ttagattcta tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga
     2401 tcaatacggc aatggaaacg gcttttttta aattagctga catcattcct tttgatgagg
     2461 tgcttccgct gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg
     2521 tagaaaaaaa catacaagca atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc
     2581 cagaccattg gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttata
     2641 tacaagaaat acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac
     2701 ttgtagcaaa tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc
     2761 ggggcgtcgc tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat
     2821 gtgcctttgt ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg
     2881 aagaagcgcc agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc
     2941 gtatccaagt ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg
     3001 caaaagaaaa agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt
     3061 gggcttttgc aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct
     3121 tgtctacaca attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg
     3181 aaacacctta tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg
     3241 cgacaggctg ttcttctatt tggggcggtg cttctcctgc ttctccttat acgaccaatg
     3301 aatgtggtca agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt
     3361 atggtatgct tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag
     3421 tattgccaaa agtatcagaa gaactacgag aattgatgga agactggtta gtacacatgt
     3481 ttgagagtga aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac
     3541 gatcaatttt ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca
     3601 gccaatggat gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc
     3661 atgtacttgc cagcggagca gacgtgaata tccttgtttt ggataacgaa gtctattcca
     3721 atactggcgg tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta
     3781 gtggaaagta tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt
     3841 atgtggcgca aattgcttca ggtgccaatc aaatgcaaac gatcaaggca tttgaagaag
     3901 cagaaaagta tcctggtcct tctctgatta ttgcctatac gccttgtatc acacatggct
     3961 tgcttggcgg aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggttatt
     4021 ggtcattata tcgatataac cctacaatgg tcgaaaaaaa caagaaccca atgactttgg
     4081 actacaaaaa acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt
     4141 cctcgctaga taacgcaaat ccaaatcttg caaaagcttt atttgaaaag actgtccacg
     4201 acgctaaaac acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga
     4261 aacgacttga accagaggaa tctatcgatg tacccgttaa gactagaaaa gaaaaaacga
     4321 cggaccttga agcagaagaa agaagagctg ctagaagagc tgccagagca gaacgacgta
     4381 aaaaaatgga acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat
     4441 aattctctga agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt
     4501 tcttgaaaat tatgcgtttt tctcaagaaa aatacgagca tatcagcctc taaaattaaa
     4561 acatatgata tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca
     4621 aatcggggaa ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc
     4681 tctgaatttt ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact
     4741 gatccaactg ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag ctatttttat
     4801 tgttatccga atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca
     4861 agtcattacg tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg
     4921 actggaacat cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga
     4981 tgagaagatg atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg
     5041 tgcgttgatc actatcgaac gtcatactgg attagatgag tatattgaga cagggattgc
     5101 tttagatgca gatatcactg gggagctgct gatcaatatc tttattccta atacaccgct
     5161 gcatgacggt gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc
     5221 tctatcagaa agtatgttga tccctaaaga attcgggact cgacaccggg cagctgtcgg
     5281 tataagcgaa gtcagtgacg cgattaccat cgttgtctct gaagaaaccg gggacgtcag
     5341 catcacgtta gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatctt
     5401 gcgtagagaa ctaattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt
     5461 cctagacggt atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc
     5521 attctttaca gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc
     5581 cagtccgttc caaatacgct ttctggaaat gaagcgtata atcaaacagt tacgggggtg
     5641 ccgatcaaga tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg
     5701 actgtcaaac tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca
     5761 cggatgttcc gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaagttccg
     5821 ctaaaaattc aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca
     5881 gtaacggttg agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg
     5941 ttcacaccat ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc
     6001 actacaggtg acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg
     6061 atggtcacag atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg
     6121 gaagtattaa gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag
     6181 ccaagcaagt cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa
     6241 agctatgatt tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt
     6301 gcatcgatcg gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca
     6361 actcgtaaaa ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt
     6421 gtggagatta cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg
     6481 acacaagaaa atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca
     6541 tcgacaacaa ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca
     6601 gaagaaacat cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat
     6661 taagttaaag gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg
     6721 gtgaagccaa caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt
     6781 atgtcctaag ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata
     6841 caagaatctc aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta
     6901 tcgaagtgtt ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc
     6961 aaaaagcaag cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa
     7021 tcaaattttt cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag
     7081 cattattaga tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg
     7141 tagaagaatt tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgatcaacg
     7201 gtgacctttc cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg
     7261 ttaatcgttt gtttgccgat cttgaaacag atttttatac aatgggcaca aatccagacg
     7321 ggttaaatat caatgacggc gtaggctcta cccatccaga acgtttagct gagatggtcg
     7381 ttgaaaaagg agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg
     7441 tggatgaact tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctaaatact
     7501 tggcagaaaa aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag
     7561 gattccacaa agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc
     7621 gttacgttgt agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc
     7681 acatgatttt cttagatttt aacacaactg gagatgggat gctttcagga attcagttac
     7741 tgaatatcat gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct
     7801 atccgcaaaa attagtgaat atccgcgtaa gtgataaaaa cggtgcgatg gatgttccgg
     7861 caatcaaaaa agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag
     7921 ttcgtccttc cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga
     7981 aagttaatta ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt
     8041 aagagatatt ttcataaaaa ataaaagaac aaggaccagt gtcagatgtc tgacactggt
     8101 ccttgttctt ttattattct actgtcactg atttcgctaa attacgtggt ttgtcaacgt
     8161 cgaatccccg taatagcgtt gcgtaatatg caagtaattg agtcggtacg actgtggtta
     8221 gagtagttag ataagggtgg acagttggta gcaccagctg gtcactttcc ttttctaatc
     8281 cctctgagac aataactagt gttttagcgc cacggctttc gacttctttc aggtttcctc
     8341 gagtatgcgc accagtcact tcttcactga tgattccgat cactggtgtt ccttcttcga
     8401 tcaaagcaat cgttccatgt ttcaattcgc cagcggcaaa accttctgcc tggatataac
     8461 tgatttcttt caatttcaaa gcagcttcaa gagaaacgtt gtagtcagca ccacgtccaa
     8521 tgtaaaaggc atttcttgta tcgctgagat atacttctgc caactcttct aaataatctt
     8581 tttgggcgat gaccgattcc atcgcattag caactaaact caattcatga gtgatatcga
     8641 atacaagaga agtttcaaat tcttttttat caccgatagc tttggctaat aaagttagta
     8701 cagctatttg tgcagtataa gctttcgttg atgctactgc gatttcgggt ccagcatgca
     8761 atagcagtgt aaaggatgct tcccgcgaaa gagtagaacc agcaacattt gtaatcgtaa
     8821 gtgaagggta gccgagacgg ttgattttta ccagcacttg gcggctgtct gctgtttcgc
     8881 cactttgact caagaaaata aagaatggtt tttttgaaag aagaggtgtg ttatagccaa
     8941 attcactggc taaatgaacc tcaacaggaa tctgtgtcaa tttttccaaa gtctgtttac
     9001 ctgctaaccc cgcatggtaa ctagttccgc aagcaacgat atagattcga tcgctatcag
     9061 acaatgtatc gaccagcttt tgatcaagag agacacgatt gtcttctgtt acgtattttt
     9121 gagcgatacg acgcatgata ataggctgtt cgtcgatttc tttcagcata taaaaaggat
     9181 aggtaccttt ctcgatatct gaagcatcta cttgtgcttc gaatgaagaa cgttctacga
     9241 tgtcacctgc aaaattttga ataataactt tttctgatgt gacggtaacc atctcttcat
     9301 cttcaatctc aacgaattcc ttcgtatgag caagcatagc cattgcatcg ctggcaatca
     9361 cattgaatcc atcgccgagt ccgattaata gtggactttt atttttagcg acgtaaataa
     9421 catcaggtgc tgtccgatca atcaaagcga aagcgtaaga acctttgatg acccgcaaag
     9481 cttttaaaaa cgcctcttgc gttgtagtat ccttcgcgaa agtttcaatt agatgagcga
     9541 taatctcagt atcggtctca ccaataaagt ggtcatttgc taaataagca attttaagtt
     9601 cttcaaagtt ttcgattacg ccgttgtgaa cgagcacgaa gcgtccactt tgggaagtat
     9661 gtggatgggc gttttcttca gaaggttgcc cgtgagttgc ccatcgtgta tgaccaatac
     9721 ctatatttcc tgtagtatca atatttattt tgtttttcaa attttgaatt ctgccttgtg
     9781 ctttgaccag ataatcagtt gtgccatctg agacaaagat accagctgaa tcataaccac
     9841 ggtattctaa tttttctaga ccgtcaatca atcctggtgt tacatttttt aatccaatca
     9901 ttccaacaat tccgcacata aaaaacatcc tactttctat aatgaattcg tatgaatgct
     9961 tccagctgca ggaaaatcct tttgttttat ctttgcagac attttttgtg gattttctgt
    10021 agagtgcagt ccctagggcc atccgccgaa tctttcgata aaccctctcc tcgtcatctg
    10081 tttacacaga cctggcgcta tcctaaaagg tataactatc ctcctttttg ttttttatct
    10141 ggaaacattt aaaatcataa ccttttgcga atgttcttgt caacaaaaaa agaataaaaa
    10201 ataaattggt atatatcaaa ttctttgtca aatgtccttt ttatgtcaaa aaataatggg
    10261 tttttatcta tcatcttttt caggagttcg gtacaataga aagtaatcgg atagaggagg
    10321 agtaaaaatg gattcaaaaa ttgagatcat gacactggga atgttaaaaa aacaattgtc
    10381 caaattcgaa gcatcagctg gtgtatcaga tgatacgaaa atatttttag atactggctg
    10441 ggatagcatc caagaaatcg cacctgatgc cttggaagtt gctcaagctc gtgagtttac
    10501 ggtagaagat gaattaacaa aagagtcgtt ttccgggtat gcgcgggaag aaaaagcaga
    10561 gcggttcgac acttctgagc agtcagaaac tgtgatcgtc ataaaaaact tgtattagaa
    10621 aggagaaaga atgaataaaa gacgttgggc agcagttctg attgctgcag gattatttgt
    10681 ggtttcgtta gtttcagcta gtttgactaa gcctcaggaa aacgatcaaa ttgagggatt
    10741 gaacagttgg ttatatgggg atgaagaact agctccaaat gtgttggaag agggagacag
    10801 tacgcaaaaa atcgtaaaat tatcagtaga tggaacgatt gctgcgggag ggtcttcagg
    10861 tttatttgct tctgaaggtt acaatcataa aaattttatg gaacaactga aggcgattga
    10921 agaagatcca acagtcaaag gtatcttttt agaagtaaat tctcctggtg gcggtgtcta
    10981 cgaaagtgcg gaaatcgcta agaaattgga tacgattcgt aaagaacatg atatcccaat
    11041 gtatgtcagc atgaaaaaca tggcagctag cggtggctac tatatttctg ctcaagcaga
    11101 taaaatcttt gcaacagaag aaacagtgac cggctcaatt ggtgtgatca tgtcaggatt
    11161 gaattattca ggacttctag aaaaactggg tgtggaagat acaacagtta aaagcggagc
    11221 gttgaaagat atgggttcag gaacgcgacc agagacaaaa gaagaagaag cggtgcttca
    11281 agcatatatc gacagcgcat atagtcgttt tgtagcaatc gtcagcgagg gacggaataa
    11341 atctgaagaa gaagtcaaaa aaattgctga tggaaggatt tatgatggtg tccaagcaaa
    11401 agaagtaggt ttagtcgatg aattgggatt tcctgaagat gctttaaaag ctatacgaaa
    11461 ggaacaacag cttgaagatg cggaactagt agaatattct tccacgccat ctgggtttgc
    11521 tagtacatgg ttaggaagta aactagcaga attccaggga ttgaagcctt ctgagactag
    11581 tcagatctta tccgttcttg agaatctagg gacagcggaa tcaccaaaag caatgtatta
    11641 ttacggaggt gagtaagcat gtcagagctt tcaaatgaaa ataaggaaca agaaaaagat
    11701 aagcaacagc agcctgctgt aggacctgac tccaactttt cccaaaaggt actgagctct
    11761 tttgaaagag agcctttgac gcccgaagaa atcaaaaaga aacaacgtaa atggaaaaag
    11821 tatacggaca caagaaatga accgccaaaa gcaaatgatt ttcctaagta tttctatgca
    11881 ggttttttta tccgaatgtt tgccttcttg gtcgatcttt tgtgtatcgg agctattacg
    11941 cgtttaacac ttggtattgc caaaaatcta ggttggctta atttttcaga ttcttatctg
    12001 agtctctacg gattcacagc cttacttatt tatctagcgt attttatttt attgacgaaa
    12061 ttcaatcatg gccaaacgat tgggaaaatg atctttggta ttcgagtcat cagctttgat
    12121 gaagaagaac ttagctggtc aaccgtttta gttagagaag gtgcttgccg gttcattcta
    12181 aaatttcctt tgctgatgtt agggtatctt ccagcagtct ttaatcagaa aaagcaacat
    12241 ctgggcgatt ttttcactaa tacaagtgtt gttacgatca atctgatcaa agcgttcaac
    12301 caggaagtca acagctgatg agcaataaaa agcagtaaca gttgaaagca ccttctaaaa
    12361 gtcagatcta aaatctagct tttagagggt gcttcagtta tttttaattg tcatgctttt
    12421 tttgttttgc tactattgaa catgagctga atatgagcaa gaggaggaca ctgcgtgaag
    12481 ccgattgatt ttccagaaaa atatgagaat ttaatgcgag tggcacaaca agcactagca
    12541 aaccagcaat atcagcaggc aaaagaatta tttcaacgag cttacgagtt gaaagaaagc
    12601 tttgaggcta atagcctgct tgttttttgt ctttacgagt tggacgagaa aaaagaagca
    12661 ttgaagcaag cactcttgca tgaaaaacag tatcttcaaa atgaggagtt tgctgaattt
    12721 tattttgact tgttaatcag tacacaagat tttttatatg ctagaaaatt gatagcaagt
    12781 acagattttt atgaagcttt cgagcagcgg gtcattgaaa aaatccaatt tgcagaagaa
    12841 ttatccggac aaatggagag gcagaagatc aaggcactcc acaataaatc ggaggaactt
    12901 ccttcgttgg aacctgcacg tcagcttgcg tctattgatg agatcgaaca gcttccttat
    12961 catgagttta tccaaacagc gagtaaactt attgtatcgc cagaggtcca tattttagca
    13021 agggcaaaat tactggagac acttaggcaa ttgaacgaat gccatcctgt tttctatttg
    13081 acgattgaag aaaagttagt caaagtcata ccaaaagatc tgccaaagcc gcagcagcag
    13141 gcaagttacc agcaattatg tgtattttca gatcgttacg ggaatgaaga tgcgctgctt
    13201 agttccgtgt taaaagaaga attcacgttg caatctgcaa ttgtttatcc tgtctatgat
    13261 acatacatcg aagacccgaa gcgctggttt caactgactg tagaggcgtg tacaggaaaa
    13321 acgatgtatg aaggtacaga agatgaaaaa caagattttt taaaaaaaag agaaaaaatt
    13381 ttgcagcaaa tgatcttctt ccattagatt ttatcagtga aaaagataaa aatgttatga
    13441 aaaacaagga ttctatccat tcaatatgtt tacaatttga aagggttagt gtactattta
    13501 aaggtatgca tgaaacgttt ttgatgtata aaatgaattt ctaaactcgg agggaattaa
    13561 atgactgcaa catttgaaaa aaaaggcacg aatgatggtg ttttaaaatt tacgattgcg
    13621 caagacgaaa tccaaaaagg attaacaact gcgtttaata aagtgaaaaa aagcttgaac
    13681 gtacctggat tccgtaaagg gaaagtttct cgtcaagtat tcaaccgtat gtacggtgaa
    13741 gaagctttat atgaagatgc attgaatgct gtattaccag aagcttatga agctgctgtc
    13801 aaagaagctg gcatcgatcc tgtttctcaa ccaaaaatcg atgttgaaag catggaaaaa
    13861 ggtcaagact gggtaatcac tgctgaagta acagtgaaac cagaagtaaa attaggacaa
    13921 tataaagatc tagttgttga aaaacaagac cgtgaagtaa cagatgctga cgtggatgca
    13981 cgtatcaaac gtgaacaaga agcacaagct gaattagtta tcaaagaaga tgctgcagct
    14041 gaaaatggcg atacagtagt aatcgacttc gaaggctttg tagatggcga agcgtttgat
    14101 ggcggaaaag gcgaaaatta ctcacttgaa ttaggttcag gctcattcat tccaggtttt
    14161 gaagatcaat tagttggtca taaatcaggt gaaagtctag atgtaaatgt aactttccca
    14221 gaagattatc aagcagaaga tcttgctgga aaagaagcag tcttcaaagt gacaatccat
    14281 gaagtaaaag caaaagaagt gcctgaatta gatgacgaat tcgcaaaaga tgttgatgac
    14341 gaagtatcaa cattagacga attgaaagaa aaatatcgca aagaattagt agaaacaaaa
    14401 gaaaaagcag cggacgatgc aaaagatgaa gcagcgatcc gtatggctgt tgaaaacgca
    14461 gaaatcgttg aattgccaca cgtgatggtt catgatgaag tacatcgttc aatggacgaa
    14521 ttcttgaaca acatgcaacg tcaaggtatc tctcctgaaa tgtactatca attaactggt
    14581 tctacagaag aagacttgca taaacaattc gaaggtgaag cagaaatgcg cacaagaaca
    14641 aatcttgtga tcgaagcaat tgcagctgct gaaaatcttc aagcaacaga agaagaaatc
    14701 aatgcagaaa tccaagaatt atcagaaaca tacaacatgc caatcgaaca aatcaaacgc
    14761 gtgttgacag aagatatgct ggaacatgat gtgacaatga aaaaagcaat cgaattgatc
    14821 acaggtacag cgacagaagc gaaagaatag aaatcaagta ataaatcatt tctgaagaag
    14881 ctacttctag aaaattgttt attcgaaata agggactgtg acacgatttt gtcacagttc
    14941 cttatttttt gttaagagta aaaaatgcaa aggtataggt cagttgatcc tttgttaaac
    15001 actttttctt tttactaaac tatgttatga tagtaaaaga ctgtaaagtc tttgatagtt
    15061 tcttgcatac tttttgcaag ttaaggggtg aagaccatgt atgacaatcc aagtagcaat
    15121 gagaccgtac gctgttcttt ttgcgggaaa acccaagaag aagtgaaaaa gatcgtagct
    15181 ggtccagggg tctacatctg taatgaatgt atcgacctat gtaaagaaat cattgatgaa
    15241 gaattttatg aagaagctgt acgcgaattc actgaagtcc ctaaaccact agaaattctt
    15301 gaagtattta ataactacgt gattggacaa gatcgcgcaa aacgctcgtt agctgttgcc
    15361 gtatacaatc actataaacg tgtcaatcag caagcacaag aaaacacaga tgatgttgaa
    15421 ctgcaaaaaa gcaatatctg cttgatcgga cctacagggt ctggtaaaac atttttagca
    15481 caaacattag caaaaacatt gaatgtacct tttgcaatcg cagatgcgac aagtttgaca
    15541 gaagctggtt atgtggggga agatgtagaa aacattttgc ttaaattgct tcaatctgct
    15601 gattacaatg tggaacttgc agaaaaaggg atcatctata ttgatgagat cgataaaatt
    15661 gcgcgtaaaa gcgaaaatgt atctattact cgtgacgttt ccggcgaagg tgtccaacaa
    15721 gccttgctga aaattctaga aggaactgtt gcaagtgtgc cacctcaggg cggacgtaaa
    15781 catccacatc aagaatttat tcagattgat acgacgaaca tattgttcat cgtaggtggt
    15841 gcatttgatg gcatcgaaac gattgtgaag aatcgtatgg gcgaaaagac gattggattt
    15901 ggtacacaaa accaaaaact ttctgaagac gaaagtgtca tgcagcatat cattcctgaa
    15961 gatttactaa aattcggatt gatccctgag tttatcggac gattacctgt tatggcagct
    16021 ttagagaaat tgacaacaga tgatctggta cggatcttga ctgagccgaa aaatgcgcta
    16081 gtcaaacaat atcaaaaact tctttctcta gataataccg aacttgaatt cgaacctgaa
    16141 gcgttgagag ccattgcgaa aaaagcaatc gaacgcaata ccggtgctcg aggacttcgt
    16201 tccatcattg aagaaatcat gatggatgtg atgtttgata tcccttcaaa tgaaaacatc
    16261 gaaaaagtga ttatcacaaa agaagcagct gaattgtctg gtgagccaac agtaatctat
    16321 aaagattcag agaaaaaagc gggttgatga aaagaggctg ccgtaaaagt cattttggct
    16381 tttgcgacag ccgcttgctt tattcatcac cttgttaagg agagagacca tgaaagtcca
    16441 tcaagctgaa attatcataa gtgcagtggc accaaaacaa tatccagata cacaattgcc
    16501 agaaatagct ctggcaggac gatcaaatgt agggaaatct tcttttatca acacattgat
    16561 tgatcgaaag aatttagccc gtacttccgg taagccagga aagacccaga cattgaattt
    16621 ttatttgatc gagaacgcat tacattttgt cgatgttccg ggatatgggt atgcaaaagt
    16681 atccaaaact gagcgagcaa aatggggaaa gatgatcgag acatatttaa cgactagaga
    16741 ccagctacgt gcggtcgttt ctttagtaga tttgcgtcat aagccttctc aagaagatat
    16801 ccagatgtat caatttttga agtattatga tatccctgta attgttgttg ccactaaggc
    16861 agataagatc cctcgtggta aatggaacaa gcatgagagc gaaatcaaaa aagcgttgga
    16921 ttttgatcgc aatgacacgt ttattctctt ttcttccgtt acaaaacagg gaaaagaaga
    16981 agcgtgggca gcaatcgaac aagcaattga atcataaaaa gaataaaaaa gacttaccag
    17041 tcaaactctt tcataaaagt ttggctggta agtctttttt tgagcttaac ttacttttgt
    17101 ttcttgtctt ttttcttttc attttgtggc tgtggtttta gaaatcgatc gatctcttct
    17161 tttggtgcaa ctgttttttc cggatcgaca gcaaatgatt cgaaaagttt atctaaatta
    17221 tctgtacgtt caaatttcaa ccccattgta aacacctctt tgtttctttt tacttagtct
    17281 atcatgtaat cgtccaaatt ggtttctaag acataaagtt ttagtttttc tttatcattg
    17341 gttataatta aattataaaa agatttgaaa gaggtgagaa aatgattggc gaagataaat
    17401 tattgatgac tatccagctc acagacttaa atgaagaaga aaaaaaacag gaaataagcg
    17461 attgggctat tttgacacgt ctgttgatcc agccaacatt tatccgctct ttcacacaac
    17521 aagcggatcc gtatgatctt cgaaagcttc aagaaaaaat aatgctagca tcggtaagag
    17581 acgaaagttg gttagttgta ggaaatgacg agaacgaatg tagttttcgc ttggaagata
    17641 atcaattatt gataaaaaat gtattgtcta tctctgtttt caaagaaaag caatttttga
    17701 ttcgagactt cattcagaaa aaaatgattg ctcatggggt gttcgcctat atgcgagctt
    17761 atagcgagtt tatctatcac aatacaaaac agatctctca acggctattg tttgagaaaa
    17821 aggatgaaat cgaacattta cctaaaatga aacaacaaaa cggggaagta gtagtcgatt
    17881 gcaaccaatt tccaggatac gatctttttt accaaggttt atgtttcact tcgtgttggg
    17941 aaatgtatta tagtcgctat tatcatcaaa tcatccctaa accgattttt ctcgacatcc
    18001 aacaagtaga aaaagtgaag gaattagaaa acgaagtcat atgtatccag ctttatcgag
    18061 acccctttaa ctggcaaaag ccaaacaatc aaatgtttca atcctatttt agggaccagc
    18121 ttgggtttga tcatcttgct tgggacaatg gtgtcggttt attgaaaccg ccattcgttg
    18181 aatacattta cactgaccat acgatccaat ctgtccagta tcagaatgca caaatgcaac
    18241 ctgttcctaa aaagaatgct tcattttttg taacaaaaag ttatgatatc caacaaggag
    18301 attataaaga aaggcgtgtt cgcggaacac taaatgctca ggcgtatttt ccttgggttg
    18361 acgagaatcg atcacgcatg atgtgctata aagttatcga tccgactgtt gctttggata
    18421 atggaatcga agcttattgt tattatatta gggaatatct agaagtagag gtaacagatg
    18481 aaaaatatca agagtatctt ttatccttaa gaatctatgt tccttcagaa tccctgtcag
    18541 aattaccgtt aaaggaaatc aaacatagac tctctgatgt taccttcaaa cgaataaaga
    18601 aaaagcggcg aagtatccaa ttcgatgtca agaaaggtga gcagcatctg cgtgtgcaat
    18661 ttttagatta ccgagaatta gaacaattga ttactttaca aaaaatatga ggtgaagaaa
    18721 atggataaac aactctatta tcgaataatg gttccggcat cattattatc cgtctgtctc
    18781 ttttttgttc ctaattttcc tattcgatca gtcatcggtt ttttggtcat gtttggcggg
    18841 atgggacttt attattatct tcttcataag gaaagaaaac ctgcttctgt ttatatgaaa
    18901 aaaacaagaa gcgaaaataa aaaacgataa aacggcttga tgatagacaa aaaaataaaa
//
LOCUS       DAZUKZ010000044        18913 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000044 DAZUKZ010000000
VERSION     DAZUKZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.044"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..1163)
                     /gene="recA"
                     /locus_tag="ACG8EF_001501"
     CDS             complement(114..1163)
                     /gene="recA"
                     /locus_tag="ACG8EF_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFS4946814.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1272..2513)
                     /locus_tag="ACG8EF_001502"
     CDS             complement(1272..2513)
                     /locus_tag="ACG8EF_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFS4946815.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2753..3331)
                     /gene="pgsA"
                     /locus_tag="ACG8EF_001503"
     CDS             complement(2753..3331)
                     /gene="pgsA"
                     /locus_tag="ACG8EF_001503"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFS4946816.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3540..4463)
                     /locus_tag="ACG8EF_001504"
     CDS             complement(3540..4463)
                     /locus_tag="ACG8EF_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFS4946817.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5202..6488)
                     /locus_tag="ACG8EF_001505"
     CDS             complement(5202..6488)
                     /locus_tag="ACG8EF_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS4946818.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6481..7749)
                     /locus_tag="ACG8EF_001506"
     CDS             complement(6481..7749)
                     /locus_tag="ACG8EF_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS4946819.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            8001..8462
                     /gene="mscL"
                     /locus_tag="ACG8EF_001507"
     CDS             8001..8462
                     /gene="mscL"
                     /locus_tag="ACG8EF_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFS4946820.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8522..8968)
                     /locus_tag="ACG8EF_001508"
     CDS             complement(8522..8968)
                     /locus_tag="ACG8EF_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4946821.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(9061..9306)
                     /locus_tag="ACG8EF_001509"
     CDS             complement(9061..9306)
                     /locus_tag="ACG8EF_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFS4946822.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(9421..9999)
                     /locus_tag="ACG8EF_001510"
     CDS             complement(9421..9999)
                     /locus_tag="ACG8EF_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4946823.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(10076..10606)
                     /locus_tag="ACG8EF_001511"
     CDS             complement(10076..10606)
                     /locus_tag="ACG8EF_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFS4946824.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(10610..11470)
                     /gene="rlmB"
                     /locus_tag="ACG8EF_001512"
     CDS             complement(10610..11470)
                     /gene="rlmB"
                     /locus_tag="ACG8EF_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFS4946825.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(11463..11858)
                     /locus_tag="ACG8EF_001513"
     CDS             complement(11463..11858)
                     /locus_tag="ACG8EF_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFS4946826.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(11855..13267)
                     /gene="cysS"
                     /locus_tag="ACG8EF_001514"
     CDS             complement(11855..13267)
                     /gene="cysS"
                     /locus_tag="ACG8EF_001514"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFS4946827.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(13264..13800)
                     /gene="epsC"
                     /locus_tag="ACG8EF_001515"
     CDS             complement(13264..13800)
                     /gene="epsC"
                     /locus_tag="ACG8EF_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFS4946828.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(13886..14125)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14122..15582)
                     /gene="gltX"
                     /locus_tag="ACG8EF_001516"
     CDS             complement(14122..15582)
                     /gene="gltX"
                     /locus_tag="ACG8EF_001516"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFS4946829.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(15752..16909)
                     /locus_tag="ACG8EF_001517"
     CDS             complement(15752..16909)
                     /locus_tag="ACG8EF_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFS4946830.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(16943..18313)
                     /gene="radA"
                     /locus_tag="ACG8EF_001518"
     CDS             complement(16943..18313)
                     /gene="radA"
                     /locus_tag="ACG8EF_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFS4946831.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(18376..18903)
                     /locus_tag="ACG8EF_001519"
     CDS             complement(18376..18903)
                     /locus_tag="ACG8EF_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFS4946832.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         5000 a         4174 c         3392 g         6347 t
ORIGIN      
        1 tatgtaccca ctcgtataat taaataactc acgatttctt tattcaataa aaaaacggca
       61 ggtcaaggag agtacctact cttcagacca gccgtttttg tttatcataa tttttattct
      121 tctactaaag gcaattcttc ttgtcctttt tcatcttctg ggacagcgac ttcttcacca
      181 ataccataag ctgctctaac tagagcagaa acttctgcca tcatttcagg atgattagcc
      241 atatagattt tagcgttttc ccgtccttga ccaatccgat cttctttata agaataccaa
      301 gcaccgcttt tatcaacaat gtctttttct actgccatgt ctagaagttc tccttcttgt
      361 gagatcccta aaccatacat gacatctact tcagcgattt tgaaaggagg tgctactttg
      421 ttttttacga ctttgatctt tgtacggtta ccaacaatat ctgtaccttg tttcaattgt
      481 tctgcacgac gtacttccaa acggatcgtt gcgtagaatt tcaatgcgcg tcctccagga
      541 gtgatttctg gattcccaaa catcacgcct actttttcac ggatttggtt gatgaaaata
      601 gcgattgttt ttgttttgtt gatcgaacca gagagtttac gcaatgcttg agacatcaaa
      661 cgtgcttgta acccgacatg tgagtcaccc atttcgccgt cgatttctgc tcgtggaact
      721 aaagcagcaa ctgaatcgac tactacgata tctacagccc cacttgatac taaagcatca
      781 gcgatctcta gaccttgttc tcctgtgtca ggctgtgaaa gaagtagttc atcgatattc
      841 acacctaatt tttgtgcata ttgcggatct aacgcatgct cagcatcaat gaaagcggcc
      901 gttccgccat ttttttgtac ttctgcaata gcgtgtagtg caactgttgt tttacctgaa
      961 ctttcaggtc catatacttc gatgatacgt ccacgggggt aaccgccgac acctaaagcg
     1021 acatctaacg caagagagcc acttggaatt gtagagattt gttggtcgat tttttcgccc
     1081 aatttcataa tagagccttt accatagctc ttttcaattt ttttcagtgc agcatctaag
     1141 gctgctttac gatcatctgc caatacgata atcctcctta tatttacaaa tcagagcaac
     1201 atttcattat ccttatcata tcttttttag tgaaaaaaag caagaaaaaa ccgaacaaat
     1261 attcgcattt atcattttct taaaagctgt tttctcaaaa gatcaaatcc ttgcataacc
     1321 gcattatatc tgatagcagc acgatctctg gcaaagtgat atttttccga aaccacgccg
     1381 tctttagatg aaatagcgat ccatactgtt cctgcagatt gaccttctaa cgtgtccggt
     1441 ccagccactc cggtgaaagc tacactgtaa tctgttcttg ctatctcacg tgcttgaatg
     1501 gccatctgct ccacacattc tttgctgact gttccatatt ctgctaatag ctccggatca
     1561 atctccagaa aaccagcttt tgtttggagc gagtaggtaa cgaatccgcc tggaaatact
     1621 tcagatacac cagcaatgtc gcctaatgcg gcttggaagg cgccggctgt caaactttcc
     1681 gctgctgtta cagtctgctt gttttctttc agtagttcca ctacgacttt tgctagcgaa
     1741 ttatcatcgc cgtatccata aaaatattcc cctactcgtt cttggatttt ttcttctaat
     1801 gctaaaagag cttgattccc tgcctgaaca tcatttgttt tcactgtcag tcgtaatgtc
     1861 acttcatttg gtttggcata tggtgcgatc gttggattga tctgtgtctc aatcagatct
     1921 tttagctcag tgaccagtcg cgattcacca atcccataaa agcgcaaaac tttagaaatt
     1981 agtttttctt cagaaggaaa acgttcctct agtaaaggac gtacttgttc cacaaacatt
     2041 ggtttcaact cattaggcgg accgggaaga aggatatacg catgtgtatc tgtttgataa
     2101 aaagtcccta aagcaagccc tgtacgattt ggtagaggaa ccccgccttc gataatctgg
     2161 gactgttgca gattgttttt ggtcattttt cgatgggttg tttcaaaata tgccaataat
     2221 tttttgtatc cttccgtatt ttgtatgaga gacttaccga ggtgagccgc agttgcttct
     2281 tttgtcaggt catcttctgt cggtcctaat ccgccacata agagtatgag ttcacttctt
     2341 gtttcagcta gagcaatcaa ctcttctaaa cgctgctgat tatctcctac aactgtttga
     2401 taataaactt cgatacctag atcagctagc tgttctgaca agaaagtggc attcgtattc
     2461 acgacttgtc ctagtaaaag ttctgttccg acagctatta tttctgcttt cattacttcc
     2521 cgctccctta tatagtatac gctgtatttt tatatttttt tatttaaaaa gagggcaagg
     2581 agctgtgaca aaaatcttgt cacagctcct tgttccaaat aaacagtgtt ctcctgccaa
     2641 aacctcggtc ataagtcaca atatacataa aacatgaaaa gctgtttttt gaaattatcc
     2701 cttgtgactc agagttagac ggcaggctca caacctctcc actaaacgat acttacattg
     2761 atcctttgaa cacaccgctg tttttgatga agtaatccac acctgaatag atagtaaaga
     2821 tcagacaagc ataaagcatg atttgatcta ctggaatacc gatcaggctg aatgggatat
     2881 tattgataaa cagcaagata atagcaacca tttgtgtagc cgtcttgact tttcccggcc
     2941 aagctgcagc catgacttct ccatgttcga caagtagtaa acgcaagcct gtgacggcta
     3001 attcacgaca gacaatgatc gctactaccc aaccaggggc ctttccttgg tcaaccaaca
     3061 tgataaaagc agtcataact agcattttat ctgccaacgg gtccgcaaac ttgccaaaat
     3121 tagtgacaag gcctcgcgca cgggcaattt ttccatctaa ccagtctgtg atactagcta
     3181 aagcaaaaat aactgcacct actagctgtg tgacagccag tgtggtgtct cctacgttaa
     3241 attgtcccca atctaaaggt aaacctacaa ccaaaatgaa aatcgggatc atacaaattc
     3301 taagtacagt taatttattt ggtaaattca cactatcgac tcctttcttc cctattctac
     3361 gtgactatca gcgaagtttc caccgatatt atttactttt ccaaatgaat ttattcaaag
     3421 gagtattttt attgaaattg atctttttga attaacgaag aaggaccgcg acacagattt
     3481 gtcacgatcc ttctttttta tacctacaag taaacagatg cgtaaactaa tttatacgat
     3541 cattctgcgt attgaagagt aaggttcaca ttttttggac tctgattgtt ttcccctgga
     3601 ttaactggta cggattctcc attcgctttg atcgttgcat tgcttgctgc accaatcgtg
     3661 atggtcgcat ttgttgctgt ttccggtaac acggtactct gtgtttcatt tgctgccagt
     3721 gtcccttgat aaacataggc attgtctact agcacaccta accatacacg attgacagca
     3781 gtaaattcaa gggttacagg cttggtagcg tctgtgatat ttactgcgat ggcactgcca
     3841 gaatcttctc ctgtagctat tgccattttc ttttcttcac ttgattttgt tgttgattct
     3901 actgtagaag actctttggt ctgcgtgctt tgctctgagg aagaggactc ctcgactgat
     3961 ccttctactt taagcgaaga agctgtgtct ccgattattg gatcagcttg acgatcttgc
     4021 caagtcatat agcccacgac aacgacaatc gccaaagcaa ccaatcctaa taaaatgaca
     4081 ggaagacttg tccaaaagcg acttggatgc gattcttcct gatgcattgc ttttctagaa
     4141 gtacttactg tttcaggctg cggacgttca gggaccgatc ctgtcaattt gcttttccca
     4201 tcaaaaacat cgactaaatg gtctccatct tctcctacag cttcagcgta ttgccgaata
     4261 aatgcccgca cgtaaaattt accaggcaat tggtcaaaat catcattgtc gattgcttcc
     4321 aaataacgtt tttgaatttt tgtaatctgc tgtaattcat ctaaagatat ttttttattc
     4381 aggcgtgctt gccgtaattt ttctccgatg gtttcaaaag ccactcgttc atcttctcca
     4441 gtctgtttga ttctccgaag catctgctgt tgcaacgaaa gacgttatgt ttgggtgctg
     4501 tgacaaaagt catgtcacag ctcctttttt ccgaataaac attgttctcc tgccaactcc
     4561 tcggtcacaa gtcacaatat ccaataaata taagaagctg ttttttgaac ttgttccttg
     4621 tgactcggag ttaaacggca ggctcacgac ctctttctta aacggtgttc aatcagctgt
     4681 tcctcggtat tagctcaaaa accggcaaaa tataaggaga tatttttccg tatgtttctc
     4741 taatactcag gtcaaaacgc tgatttcaca acctcttcct taaacggtgt tcaaaagctt
     4801 gcatctgcgg taataagtcc aaccaagtta aaaacattaa gaagctgttt ttacttggct
     4861 gttcttatta cttgatgcag ggcagctttt tcacaacctc ttggtacacg gtgttccaga
     4921 agtagcttct tcggcaataa gccgaaatat cacaaaaatt tgagaagcaa ttttcgtaaa
     4981 ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctctttgatt acttcgtttg
     5041 attttatttt aatataagtt gattaaaaga gagcatgatt tgttttattt tctcactaat
     5101 ttttccaaca caactaagaa taccataaaa gctgattttg gaggaggttc ctactacaag
     5161 tctaaaacga aatttaagac ttttcctcat aaaatactgg tttattttgg tctcatgtaa
     5221 aactcgctga aggcctcttc acttataaat gcttccccag ctgccagtac gtctttcatt
     5281 tggatcgaat cgatgatttc aggaagatca aataaagttc gatcgccaaa taaactttgc
     5341 gtaaattgat tggcaatata ttcgatcgaa tttaatgatt ggaaatattg tccaagcatt
     5401 ttcttcttca gcaaatcgag atttgtttct gacacttccg gatcatcagc gaatccaaga
     5461 atgatttctt tgactttttc tgctgctttc tctggttcgt ccgtatctcc gctgaagtcc
     5521 gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa agctgtcatc gataattcct
     5581 tgattgtaca tcgctagata gtttcgagaa gtattgccta aaatcatttg gaacaacaga
     5641 ttgattgctg ttttatagcg taacagttct gttccttctt gcggcaatgt gtctaatcct
     5701 ttaataccaa gtacaaattt atctcgtgta atggctgctt ccaatgcact ttgtttgatg
     5761 atttcttttg tgttttcagg gaaataacgt acaatttctt gctttggagg gaaatttttt
     5821 gcttcttggt tttcccgaat aagtttcatc aatttctcag gctccatctt accaactaca
     5881 aataaaacca tattgcttgg ctgataaaac gtccgatagc acgtatacaa gtcttgagct
     5941 gtgattttgt cgatactttc aaccgtgccc gcaatatcga tatgcaatgg gtgtgtagga
     6001 tacaagttat ttagtatgcc aaaaaacatc cgccagttag ggtcgtcttc atacatttgg
     6061 atttcctgac cgatgattcc tttttccttg tttacagttt cttctgtgaa gtaaggtgct
     6121 tgtacaaaat cgatcagagt cgtcagattc ttttctacct gatccgtcgt agaaaacaga
     6181 tagctggttt tcgtaaaact tgtgaaagca ttggcggaag ctccttgttt cccaaataat
     6241 tgaaagacat caccatcttc tttttcaaac aacttatgtt ctaaaaaatg ggcaattcca
     6301 tctggaactt tcaccttttc tttttcacca tagggaataa attcgttatc gatcgaacca
     6361 taattcgtac tgaacaaacc atatgtcttg tgatagtcat ttttaggcaa caaatacacg
     6421 gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat tgatttgctc atattctgtt
     6481 ttattcattt gcatttcctc ctgctaaaaa gaagattgcc tgcaattcga tttgctcagc
     6541 tacttgttgt atctctttga tcgttacgct ctccatacgt ttcaaccatt cttcatcaga
     6601 taattttgtt tccggcagcc agctgtccaa aaaagaagct tcgattgccg cttgcggaga
     6661 gtctagagaa agcagatatt ggtttctcag cattgctttt gtctgagaca gttccaactc
     6721 tgttatttca ccatttcgca aactttctaa ttgctcgtga atcagatgaa gtacttgatt
     6781 tcggttcttt tcatcgatac cggtttggac actcatgaat cctcggaacg tatccacgct
     6841 actagaggca taataagcta gactttcttt ctctctgaca ttcatgaaaa gtttagagtg
     6901 aggaaaaccg ccaaataaac cgttaaagac cattagcgca aaacgttctg gttcatcgta
     6961 atacacattt gtctgataag ctaggttcag ctttgcttga acgattggct cacgtacttg
     7021 tcgttccttc acgatattca cttgaggttg ggtataaaac agatctggat aaactgtttc
     7081 ccgcgactca aatggtaatt gcccaactgc ttcagctgct ttgtccggat caacatcgcc
     7141 gacaacaaaa atgtcgattt gatcatttgc catcatttct tgatagtagg acgctagcga
     7201 actagcagtg atcttgtcca aatcttcgac tacgcccaac cctgggatct tttgttccgg
     7261 gctgttttca aaataaagtt cttgcaaggc ataggaagca aaagtttgtt tatcttcctt
     7321 caaactttcc aaatatagac gcagattgtt cttttccaag tcaaacgtca actgatcaaa
     7381 ctgatgattt ttaatatttg gataaaataa aacctctttt aaaaaatcta ccgcttgaga
     7441 aaaaaggttt ggatcattga catattcacc attcacaaaa ctgatccctg cgtttagcca
     7501 atgtaaattt cctttttttc taactgataa tccaaagctt gcgccataaa gctctgcaag
     7561 ttgcgcactt aactttgtct gatccgaata attcaaacta tttgtttcca acatgctgct
     7621 gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc gcagtaaaac ggataaaaat
     7681 ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga ttcacgcctt ctgccaattg
     7741 atacaccata tttatatgct ccttttcaca gttgattgcc cgcttactct tttaatgggt
     7801 caacctgcct tttttactta ttcctctgct tgaaatttat aagttcaggc aattcgccgt
     7861 tttttctaaa tgatgttaca ctttatagta tagcatagat gttttttatc aaaagaaaca
     7921 gacggctaaa agcgtatatt gtttttttat tttttatttt tcgatatact aaagacaaat
     7981 aaaagaaggg agggtcattc atgatcaaag aattcaaaga gttcatcatg cgtggagatg
     8041 tcttggatct tgctgtcggc gttgttattg gtagtgcgtt tacaagcatc gtaaaccaag
     8101 tggtgcaagg cttgatcaca ccactaatag gttggtttgt cgcaatgatc acaggtactt
     8161 ctgatttaga aggagcactt tcaattcttg actggtcgcc gacaaaaggc gtcacatttg
     8221 cgtttggtaa cgttattagt gctatcatta catttctgat cactggtttt gtcttattct
     8281 tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc caaagaagag caagtggaag
     8341 tggaagaaac tcctactgca gaagattatc ttagtgacat acgcgatctt cttgccaaac
     8401 aagtggcaga agacaataca catccagtag agcaaacaaa tctttccgat acttctaaat
     8461 aataaagaat ctaaaaaaaa agaaagccga ttttcagtgt cggctttctt tttttagatt
     8521 cttaacttgc tacttgttcc tttttcatgt cgatatgtgg aatatcatct tccaaataca
     8581 catctgagat tgccttgaat ccaaacgact cataaaaagc ttgtaggtat tcttgtgcgc
     8641 cgatcacgat tggtttttct ggaaaatttt tctggatcat gttaatcgtt tcttgaacaa
     8701 tctttcttcc taaaccatct ttacggttgt cctgatgaac aaggacacgt ccaaaatgga
     8761 tactctccgc ttcttcatat actcgcgtgt aggcaaggat cttcccatca gtatcttgaa
     8821 aaaacgtatg ccaagcttct ccatcaatgt catcaacttc ttgataagga cactgttgtt
     8881 ccacaacaaa aactgcgatt cgttctttga cgatcgcgta aaactctgct gttgacaatt
     8941 cttcaaattt ctttacctga tattccacta agcaaacgcc ccctgctttt ttattcttag
     9001 cataccgatt cgcacacaaa aaagcaaacg aaccttacga atctcaggct cgtttgcttt
     9061 ttaaactgct tcagttaaaa attcgatctc aactgtccga gtaagtacat ctgaatagct
     9121 gtaagatact cgttcaaaag aattttcttc aggatccaaa tcaacaacga atactgaagg
     9181 ataggtttct gttaaaacgc cgcggcgttc tgtttgacgc tttcttcctg tctgagcaac
     9241 caacatgatt gggctgccga tacgaccttc taaatctttc ttgattgaag ctaaagttgt
     9301 tggcatacag ttcacctcta ctatcaacta taccacatat ttttaaaatt ttcaagttta
     9361 ccataaaaaa aaaagaaatg caataaaaat aaataagaag ctcattaaga tttttctaaa
     9421 ttattcctca ttataaaatt tcaagtgttt ttttattttg agttttactc tcccgtaagc
     9481 gctcattact tgcttgtgat ccattgctaa acattcagcc atgtctacaa aatcaacgcc
     9541 ttgcatataa taatgaaaaa ttttagcttc tatttttgaa aactggattt cgcaattttc
     9601 gattgcttct tttaataaaa gatgctgaat tgctaaaggt tcttgcaatg tgatctctgc
     9661 taaaaagtct ggtccttctg tctctatttt ttggtcgatt gagacttcat ctttttgatc
     9721 acgacgcttc agcgcgttct gcttcctcaa cagagaatag atttgattgc ttataacgct
     9781 tttataataa cttccaaagc taacaccttt gcttttttcg taagttctca atgcctgata
     9841 taaagcgatc cttgcttctt gttcccaatc ctcagaaaca taatttctta aataataggt
     9901 gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac agggcagcaa atgcttcttc
     9961 gtcaccatta cgtgcagcgc gcaataactc ctcagacata aaataattcc tccataaatt
    10021 cgtttatgga ggaatcttat catcaatgat tcatcgaact gattagaacg atatgttatt
    10081 tattttttct cattaaatcg tccaattttt gggacaattt ttttaactgc tcttcattcc
    10141 atggagaatt acgtcggaaa tcaataaaat gcatatcttt attttcttgg gctattgttt
    10201 tttccgtttt ttttatcgtc ttatataatt cataagcaga agttcgtaat gctccttggg
    10261 aaaagatcat ccactgttct gctaaatcgc ttgtcgccac tgtcacttgt gtcaaacggt
    10321 cattcagctg accagcgatt ctctcgatat aactgtcagc cgtttcgtct tctttcgtaa
    10381 aaacaacatc caactggtat ttcttataat tttgttggat gcccggaact agttgggcat
    10441 cgaagacaac gatgacttcc attccttcat atttcgcata atttgataaa cgatgtaaaa
    10501 gagcttctct tgcatccgcc attttgtcct gctttttaag ctggacaagt tccggccacg
    10561 aaccaatcat gttgtagccg tctacaatca acagctgttt tttcatggtc tactccccct
    10621 gacgttggga aaaagcctga tacataagca aaccagcagc aacactggca tttaagctct
    10681 ggacgtgccc ggtcatcggg atcgtcagca attcatccat ttcttttttc aatcctggac
    10741 tcattccttt gccttcatta ccaatgacta aagcaattga tcctttggca ttccattttt
    10801 ggaagtttgt gccggtcata tctgtgccaa atacccaaaa tcctgtttct ttgagctgtt
    10861 gcacagcttg taccaaattc gtcacgcgtg caacagggac atgttcgact gccccagtgg
    10921 aagtctttgt tacgataggt gtgattccaa ctgctcggtg ttttggtatg atcacaccat
    10981 cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa attgtgtgga tcttcgatgc
    11041 tatccaaaat caaaaagaag gggtcttctt ttttctcttt tgttcgttca atcagttggt
    11101 caagggataa atattcataa ggtgtaatcg caagaacgac tccttgatgc acgccgtgat
    11161 cgctcaaagt ttctaatttt tgtttaggaa cccatttgac tggcacagct tgttcacgtg
    11221 cgatttcttt cagttcttct actttttctc ctcgtgaatc ttcttgaaga aagagtttat
    11281 tccctcgccc ttgttgtagc gcttctactg cggcatggtg cccaaagacg aaattatctt
    11341 ctaatgcttc ttcattttga tcagaaactg actgatttcg tccagtttgc tttttatttg
    11401 aatatctatt ttgttttttg tcttcaaatc cacgtttcga ttgatgtttg tttgttcttt
    11461 ctttacccat ttttttcacc aactttctga atacaccagc ggatcaattc ttccatccgc
    11521 tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt cgaaccctgt tgcgacccga
    11581 tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt tagcatttct tcctctgcga
    11641 taataaagtt cttcttcttc tgtcaaaaga tgttcttcca gcatttgctg aatcaaaaag
    11701 gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat gcagcttatt gggttttgtt
    11761 tctccttgat caatcaggtg gtcacgaaca tagatttcgt aaatcgcatc tccaacataa
    11821 gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc ttctcttctc catcgggttc
    11881 cttgcggtgt atcttctaat aagatccctt tttctttcaa caaatcacga atttcgtcgc
    11941 ttcggacaaa atcacgattg cttcttgctt gatttcgttc ttcgatcaat tgttcaatct
    12001 cttcatctaa taactcttct gctgtaaata aaataccgaa gatcgaaagc cattgcgaga
    12061 ataaagcttc tccttgtgcc aagacagcgg cagaaacttt cggctggtct agataagtgt
    12121 tcagccactt agcaagctca tagaccactg tgatcccatt tgctgcatta aaatcatcgt
    12181 ccatttcttc gatgaatcgc tgttctaaat tttctaacaa agcgatttta tccgcatcat
    12241 ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa attgtcaaac gtattcttca
    12301 gtcgttgtaa attggtggct gcttctttca tcgtcgtttc actgtagcgg atcggacgac
    12361 ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg atcaacgttt ttgatcattt
    12421 catgtacagt gatgaaattc cctagagatt tactcatttt ttcatcgtct tcgccaatcg
    12481 ttacatagcc attgtgcatc cagtaattag cgaatttttt ccctgttttc gcttcacttt
    12541 gtgcgatttc attttcatga tgaggaaatt ctaaatcttg cccgccacca tggatatcga
    12601 tcgtttctcc taaatgtttt gttgccataa cggaacattc aatatgccaa ccgggacgtc
    12661 ctttgcccca tggtgaatcc caagaaatct cgccaggttt tgcttgtttc cataaagcga
    12721 aatctaatgg gtcttctttt tgctgctgtt cttcccctgt acgctgactt gctccgactt
    12781 ccagttcatc gatggattgg tcacttaatt tcccataatt ttcaaattta cgggtacggt
    12841 aatacacatc acctgaagat tcataagcat agcctttttc aaccaacact tgaataaatg
    12901 ctaaaatatc tggcatatga tccatgactc ttggatgcag tgtagcaggt tcgacattca
    12961 acgctttcgt atcttcttca aatgctcgga tgaaacgatc agcaacttct ggtgctgtaa
    13021 tacctaattc gtttgctgta cggatgattt tatcgtctac atccgtgaaa ttcgatacga
    13081 aatcgacttt atagcctcga tattcaaaat agcgtcgaat tgtatcgaaa gcaatggtgc
    13141 tacgagcatt tccgatatgg atataattat acacagtcgg tccgcagaca tacataccga
    13201 catgtccctt ttgtataggg atgaattctt ctttttttct ggtcaatgta ttgtagattt
    13261 ttatcattct gatctcctca ctttttctcc tttgattcga acgacttttg ctggtactcc
    13321 taccgctgta gcgtcgtctg gaacatcttt caacacaacg gcacctgcac cgattttggc
    13381 gttttttccg atcgttaccg gtcctaatat ctgcgcattc gcagagagca tggctccttg
    13441 tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg ccgcctaatg ttacgccatg
    13501 gaataaaatc acatcgtcct cgatttctgc ggtttcgcca atgacgattc ccattccatg
    13561 gtcgatgaac acgccagttc caatcgttgc tcctggatgg atctcaacac cagttaggaa
    13621 acgccagaat tgagcattga tcttcgccaa taaaaagagc cggtgctgat ataaaaaatg
    13681 agagaaacga tgccaaaaca aggcatgaaa accagggtat gtcaaaacga tctcagctgt
    13741 cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc gctcgggtaa accaactcat
    13801 gactttccac tcctttcgta agaattttat gtaggaaatc atcttcttat aaagaaaaga
    13861 agcgcctttt aaataagttt ccttatcaaa agacgctttc cgcgtggttc cacttttctt
    13921 cataatcaat gattatctct gacagctaac gcctgttata cgttcttggc tactttgttt
    13981 cctataaaac agtttttcct gttttctgtg tttcgccaag accactccca gaggcatttc
    14041 ggcaaccatt cattgatacc ttgcaccacc cggtatctct cttgtaatga tcagctgctt
    14101 acttttttct gttcatcgtg tttagttcag tacttgattt aaatgtgcta atgatttttc
    14161 acgaccaagc aattcgatcg tttcaggcaa ttctggacca tgcatttgtc ctgatacagc
    14221 tacacggatt ggcatgaaaa gattttttcc ttttacgcca gtttcttttt gaacagcttt
    14281 gatggcagct ttgacagttg gagcatctac tacttccatt tgttctagct gtgctttgaa
    14341 tgcgtggaga acagtaggaa ctgtttcacc agcaagtact tcttttgctg cttcattcaa
    14401 tacaggatgt tcattgaaaa atagcgtaga cagatcaacg atttctgcag catagctcat
    14461 ttgaggttga tacaggctaa cgatcttctt cacccattcg attgtctttt catctgggtt
    14521 ttctgctaca cgtccatcag cgattagata aggcagacac atgtcagtaa atgcatctag
    14581 atccattgct ttgatgtaat ggttgtttac ccattgcagt tttttcgcat cgaaagctgc
    14641 gggtgattta cttaggcgtt ttggatcgaa cattttgatc aattcttggt gttcgaagat
    14701 ttcgtcttcg cccacaggtg accagcccaa taatgcaata aagttgaaca ttgcatctgg
    14761 tagatagcct aattcgcgat attgttcgat aaattgcaag attgattcgt cacgtttgct
    14821 tagtttttta cctgtttcac tgttgatgat caatgtcata tgtccgaagg ttggtggtgt
    14881 ccagccaaat gcttcataga tcatcagttg ttttggtgta ttggcaatat ggtcatctcc
    14941 acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc gcaacagcaa aattataagt
    15001 tggcatacca tcgcgttttt ggatgacaaa gtcgccaccg atattatcgg attcgaacga
    15061 aatagcgcct ttcaccatat ctgtaaatgc gtattctgta ttgcgaggca cacggaaacg
    15121 tacaacagat tctagacctt gtgcttcttt ttctgcttgt tcttcaggtg tcaagttcgc
    15181 acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt tgtgcttccc gttctgcttc
    15241 taattcttct tctgtgcaat agcatttgta tgctcgattg ctagcaagca gctgatcaat
    15301 cagcggttga tagatttctt tacgttcaga ttgacggtaa gggccgtatt ctccaggatt
    15361 ttcaggagat tcatcccaat tcattcccaa ccaagcaagg ttttccaact ggcttttttc
    15421 gccatcttcg atattacgtt tttggtcagt atcctcaata cgaataataa agtctccatc
    15481 attgtggcgg gcaaacaaat aattgaataa tgcagttctt gcatttccaa tatgcaggtg
    15541 cccagttgga cttggtgcat agcgtacgcg tactttcgtc attttcttcc tcttctttca
    15601 aactagactt tgactaacag caaaaattcc gtcagttacg gcttctcaaa tatcatttat
    15661 ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa atagtaaagc caactggttg
    15721 aatttcctta ccagttggct gaatttcttt tttattttga ttgtttagtc tttttctctg
    15781 tctctttttt attgtcgatt cctcgtcctg catgtgcagg tttagcaaag atcatgcgtc
    15841 cagcagctgt ttgaagggca cttgttacaa cgacttcgat acgttcattc atgtaatgct
    15901 gtccatcttc tacgacaacc atcgtgccgt catcaaggta ggctactcct tgttggcgtt
    15961 ctgtcccggc ttttactact agaacattca tcgtttcacc agggatgacg actggtttta
    16021 cggcatttgc caacgcattg atattaagaa caggtacatt ttggaattca gagactttat
    16081 tcaagttata gtcattggtt acaatgacac catctaaaag cttagccaat ttgatcaatt
    16141 tgctgtctac ttcactgata tcttcaaagt ctccatcgta catttcgaca gatataccct
    16201 cttctttttg caaggcattt aggatatcta atccgcgacg accacgcaca cgtttcaaac
    16261 tatcccctga gtccgcgatg tattgtagtt cgtaaagaac aaaattaggg atcatcaagg
    16321 ttccttctaa gaatccagtc tttgtaatat cataaatccg accgtcaatg atcacgcttg
    16381 tatccaagat tttatattta tggaaatttt ctcctgattt tcgttctaac agttgactct
    16441 caacttgact agctgtttct tgctcgattt ttttgcttcg tgaaccaaaa actttcttcc
    16501 attcttcgat tcgtgtcgtt cccattctca atcccagata gcctaagatg atcatcagta
    16561 aaatcggcaa tacactattg acgaatggaa tttccatatt gtaaagaggg gtcgagatca
    16621 caacacctaa agtcaatccg atgatcgcac cgatcgcacc gaagagaaga taagtcaagc
    16681 tgacctcact tagtttttgt tctacttgtt tgataatagc tgagatatgt ttggccaaca
    16741 gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct gttggtaaat gtattattca
    16801 gccatccgtt tgactgttga ccgattgcct gccaagctga gggtaaaaat gaaataccca
    16861 agcttgcgcc gacaatgatc atcagcaaag taatgacacg tttttgcatc gtttttcctc
    16921 cgtttcgaca atcatttatt agttagcgga acacacgctt caacgtttca gccaaagttg
    16981 ctactccaac tatctcgatt ccttttggcg cttcccagtt acctaagttg tttttaggta
    17041 ggtatacttt tgtgaagccg agtttttgga cttctttgac tcgttgttca atatgactga
    17101 cacgtctgat ttctccagtc agtccaattt caccgatgaa acattccgtt ggctgtgttc
    17161 ctttttcttt atagcttgaa gcaatactga cagcgatagc taaatcaatc gctggttcat
    17221 ttaattttac cccacctgca gcttttaagt aagcatcttg attctgaaga agcaacccag
    17281 ctcttttttc cagcactgcc atgatcagag atacccgatt gaaatcaaga cctgttgttg
    17341 ttcgtttggc atttccgaac atagtaggtg tgactaatgc ttggatctct accaaaattg
    17401 gacgtgtccc ttccattgcc acaacgatag cagagccagt tgcaccgtct aggcgttctt
    17461 ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc atgctcatgc atttcaaaaa
    17521 tcccaatctc attcgttgag ccaaatcggt tttttactgc tcgcaaaatc cgaaaactat
    17581 gatgttgttc accttcaaaa tataacaccg tatccaccat atgttctaac attcttgggc
    17641 cggcgatcga gccttctttg gtaacatgac cgacgataaa aatggctatc ccatttgttt
    17701 tagcggtttt gagtagttct gcagtggttt ctcttacttg gctgacactt cctgccacac
    17761 ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat gataacataa tcaggggaga
    17821 tatgttcgat ggcacgactg atttcgttca tatctgtttc agcgtataga taaaattctg
    17881 tgttgatact tccgaggcgt tccgcgcgta attttatctg ctctgcgctt tcttcgccgg
    17941 atacatataa gacttttccg cctatggctg ctaattgctg agaaacttgt aacagcaaag
    18001 tggatttccc gatacctgga tcgccaccaa tcaagaccat agaccctggc accacacctc
    18061 cgcctaacac acggttcagt tcttctaatt ttgttttgat ccgaggttct tttttaggga
    18121 cgacgtctgc gatttttgtt ggttttgctt tttctcctgt caaacttgtc cgagttctgc
    18181 ggtccgttgt atcttgttcg atttcttcga ccatcgtatt ccattttccg caatttgggc
    18241 agcgtcccaa gaacttaggc gacacatacc cgcaattttg gcaaacaaat tgtacttttg
    18301 atttttttgc cattgtaccg cctcctttta tcctaatcat gagacattaa atcaagctta
    18361 agtatcttta acgttttatt tactggaaga accaaatccg ccttgacgtt gagcagtcgt
    18421 ttgatcttga tctgctttta aaaacggcaa aaagatccct tggccgatac gctcgccttt
    18481 tttgatcact acatccttga aaccaaagtt ggtgaattgg aacatgatat ggccttcatt
    18541 tgcctcatta ttgtaataat cactgtcaat cacacctact ccgtttgtca acactaaaaa
    18601 ccgcttcaac ggattgcttg aacggttagc aagctgcaaa aattcatctt ctcccatgta
    18661 agctttgatt ccagtaggca cgagggttgg tttcaattgt ttttcatttt ctgatacatc
    18721 ttttttcaat ccatctgcga ctgtttccaa cccctttttc cagcctgctt tccagatact
    18781 tggaatcacg acctcttctg ctgcttcaaa atcatagcca gcagcatgat gtgtagcgcg
    18841 ctgcggtaga ttgatatttt gatctttata tttagaaata acttcaaatc ctctttttct
    18901 caccggtttt cct
//
LOCUS       DAZUKZ010000045        18529 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000045 DAZUKZ010000000
VERSION     DAZUKZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.045"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..566)
                     /locus_tag="ACG8EF_001520"
     CDS             complement(111..566)
                     /locus_tag="ACG8EF_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946833.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(720..1928)
                     /locus_tag="ACG8EF_001521"
     CDS             complement(720..1928)
                     /locus_tag="ACG8EF_001521"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFS4946834.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2040..2372)
                     /locus_tag="ACG8EF_001522"
     CDS             complement(2040..2372)
                     /locus_tag="ACG8EF_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS4946835.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2520..3395
                     /locus_tag="ACG8EF_001523"
     CDS             2520..3395
                     /locus_tag="ACG8EF_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4946836.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3477..4058)
                     /locus_tag="ACG8EF_001524"
     CDS             complement(3477..4058)
                     /locus_tag="ACG8EF_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFS4946837.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4064..5323)
                     /locus_tag="ACG8EF_001525"
     CDS             complement(4064..5323)
                     /locus_tag="ACG8EF_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS4946838.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5325..6371)
                     /locus_tag="ACG8EF_001526"
     CDS             complement(5325..6371)
                     /locus_tag="ACG8EF_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946839.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6606..6881)
                     /locus_tag="ACG8EF_001527"
     CDS             complement(6606..6881)
                     /locus_tag="ACG8EF_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFS4946840.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7124..8434)
                     /gene="der"
                     /locus_tag="ACG8EF_001528"
     CDS             complement(7124..8434)
                     /gene="der"
                     /locus_tag="ACG8EF_001528"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFS4946841.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="ACG8EF_001529"
     CDS             complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="ACG8EF_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFS4946842.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9980..10660)
                     /gene="cmk"
                     /locus_tag="ACG8EF_001530"
     CDS             complement(9980..10660)
                     /gene="cmk"
                     /locus_tag="ACG8EF_001530"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFS4946843.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10709..11344)
                     /locus_tag="ACG8EF_001531"
     CDS             complement(10709..11344)
                     /locus_tag="ACG8EF_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFS4946844.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11410..12813)
                     /locus_tag="ACG8EF_001532"
     CDS             complement(11410..12813)
                     /locus_tag="ACG8EF_001532"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFS4946845.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12800..13831)
                     /locus_tag="ACG8EF_001533"
     CDS             complement(12800..13831)
                     /locus_tag="ACG8EF_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4946846.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13862..14083
                     /locus_tag="ACG8EF_001534"
     CDS             13862..14083
                     /locus_tag="ACG8EF_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFS4946847.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14248..14985)
                     /locus_tag="ACG8EF_001535"
     CDS             complement(14248..14985)
                     /locus_tag="ACG8EF_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFS4946848.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15138..16478)
                     /locus_tag="ACG8EF_001536"
     CDS             complement(15138..16478)
                     /locus_tag="ACG8EF_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4946849.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16715..18238)
                     /locus_tag="ACG8EF_001537"
     CDS             complement(16715..18238)
                     /locus_tag="ACG8EF_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS4946850.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         5233 a         3939 c         3120 g         6237 t
ORIGIN      
        1 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
       61 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
      121 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
      181 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
      241 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
      301 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
      361 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
      421 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
      481 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
      541 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
      601 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
      661 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
      721 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
      781 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
      841 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
      901 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
      961 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
     1021 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
     1081 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
     1141 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
     1201 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
     1261 gtttcaataa atgcatggat tcccgcggcc ccttcatttc ccaagagcat cttgatgaat
     1321 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
     1381 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
     1441 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
     1501 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
     1561 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
     1621 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
     1681 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
     1741 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
     1801 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
     1861 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
     1921 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
     1981 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
     2041 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
     2101 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
     2161 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
     2221 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
     2281 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
     2341 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
     2401 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
     2461 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
     2521 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
     2581 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
     2641 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
     2701 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
     2761 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
     2821 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
     2881 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
     2941 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
     3001 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
     3061 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
     3121 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
     3181 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
     3241 atatggtcgt tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg
     3301 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
     3361 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
     3421 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
     3481 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
     3541 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
     3601 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
     3661 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
     3721 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
     3781 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
     3841 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
     3901 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
     3961 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
     4021 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
     4081 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
     4141 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
     4201 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
     4261 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
     4321 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
     4381 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
     4441 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
     4501 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
     4561 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
     4621 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
     4681 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
     4741 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
     4801 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
     4861 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
     4921 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
     4981 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
     5041 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
     5101 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
     5161 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
     5221 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
     5281 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
     5341 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
     5401 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
     5461 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
     5521 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
     5581 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
     5641 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
     5701 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga
     5761 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
     5821 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
     5881 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
     5941 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
     6001 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
     6061 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
     6121 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
     6181 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
     6241 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
     6301 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
     6361 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
     6421 tagaaaagat aagagcaaag ctcacctcat aacttggaaa taaataaaaa ttaaataaaa
     6481 tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt
     6541 ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa
     6601 ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta
     6661 gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg
     6721 cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct
     6781 tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta
     6841 gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc
     6901 acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa
     6961 aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata
     7021 gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac
     7081 tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag
     7141 ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga
     7201 aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg
     7261 gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg
     7321 ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa
     7381 gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag
     7441 tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct
     7501 cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga
     7561 cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc
     7621 gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca
     7681 ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac
     7741 cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg
     7801 cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat
     7861 tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt
     7921 cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa
     7981 tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt
     8041 catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt
     8101 tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc
     8161 cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga
     8221 tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt
     8281 ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt
     8341 cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg
     8401 ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa
     8461 tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa
     8521 gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc
     8581 agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct
     8641 ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta
     8701 ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt
     8761 tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact
     8821 ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt
     8881 gaaatttgag agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg
     8941 aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg
     9001 atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca
     9061 ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt
     9121 tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg
     9181 tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca
     9241 tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta
     9301 tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt
     9361 ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca
     9421 aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct
     9481 tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct
     9541 aaacggcgtt ttgaaagtag gtagctgccg ttttctttat ctttgccgat cgtactgata
     9601 acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa
     9661 gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc
     9721 acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct
     9781 tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct
     9841 gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt
     9901 aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt
     9961 tttcattttt tttcttaatt taaaaaagaa gataaccttt tttcaaaaca acagctttta
    10021 ttgctgcaac gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg
    10081 cttgcttcag cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt
    10141 tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt
    10201 ctgcacgctc acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag
    10261 ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga
    10321 cagcaactaa ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga
    10381 catctggttg acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat
    10441 cttcttgttg cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat
    10501 ctgaaaaaga tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg
    10561 tatctgtata aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag
    10621 atgaagcagg cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca
    10681 gaaaaaaagc cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa
    10741 gcataaagtt gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg
    10801 cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg
    10861 cagctgatga agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag
    10921 ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac
    10981 ctgtatcttg agacacagta gattcttccg cgattgtact agattctact gttgaagtct
    11041 cagttgactc ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag
    11101 aagaagttgc tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg
    11161 ctggaatagc gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc
    11221 ctttcttttg ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg
    11281 gttgttccca tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc
    11341 tcactgtttt acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt
    11401 cctaaaaaat caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg
    11461 aagttgttca gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc
    11521 aatatcacaa caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa
    11581 ttttctgcgg cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt
    11641 ttctttttcc tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat
    11701 cagctccatc catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa
    11761 ataaagctca aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg
    11821 ttcatcccca ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg
    11881 accgatttct tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg
    11941 gatatcagat ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg
    12001 attttttaca aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata
    12061 gtatccaact gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt
    12121 tgcacaataa atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc
    12181 tttttgctgc gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat
    12241 ctcttttggt ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc
    12301 tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg
    12361 ttgatattct ggacggaaat caactcccca ttgagacacg caatgagcct catctactac
    12421 atataacgca atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc
    12481 gggactcaaa aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga
    12541 ttgcgaaagt aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt
    12601 cacttgatct tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa
    12661 ataccccgtc aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt
    12721 atcttctcca tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa
    12781 tccaaaatat ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat
    12841 agatttgata caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa
    12901 agcgccactc tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga
    12961 gaatccactc gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga tgatctgtta
    13021 ctgttcctct ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa
    13081 acatctgctt ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca
    13141 aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc
    13201 tgctttgttg cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca
    13261 gccctgttcg ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta
    13321 tctccgaaaa cagctgcacc aaactctcat acgcagtatc aatcaaaatt tcacgtggca
    13381 attggctttc actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg
    13441 gaagatagtc ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga
    13501 gccgccaact agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta
    13561 tttgatgttc ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta
    13621 tttcgatcca accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca
    13681 attctggaaa actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg
    13741 aagtcgttcg ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg
    13801 atgaaaatag cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga
    13861 aatgtcacta ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat
    13921 cgaagcgcca gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc
    13981 agaggctcat caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca
    14041 aaaatgtcca gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta
    14101 aaaacgtaca ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg
    14161 tgaaatcccg acttattttg aaagattcat ttgctatcta tagttaattg actagttttt
    14221 tcacggaccc tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt
    14281 attgacgatc acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg
    14341 ctcaccatag ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct
    14401 ttccggtaaa tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt
    14461 cgccgtgatc gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata
    14521 acttgccatg aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg
    14581 acttgccatt acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc
    14641 ttctttgacc ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac
    14701 aatagcgact gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg
    14761 ctctacgaga gcaccaggat aaaaaataag taatggagac tttccatgat tctttttctc
    14821 tccttcaaaa aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc
    14881 tgacgatggt tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc
    14941 taaaatgatg actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt
    15001 ctatctgagt gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag
    15061 aaatgcaaac tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc
    15121 tttttgtatg tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc
    15181 attgctttat acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga
    15241 gtatcaggga acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg
    15301 ccgcctgata caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga
    15361 tttttcacta actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca
    15421 taagtcaacc catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg
    15481 aatgtaacag ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa
    15541 cttgcaaagt tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct
    15601 tcaccagttg cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat
    15661 acttctgaaa gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc
    15721 gcgtggatac ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg
    15781 tgttttttca atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca
    15841 ggaacaacat tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc
    15901 aagtaagctt gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca
    15961 aatccttgcg taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt
    16021 tcttcatctg taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc
    16081 aaagctttga cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga
    16141 ccaactaacc agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg
    16201 tcccctgctg gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca
    16261 atctctgaat aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca
    16321 ctgatttcca atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt
    16381 cctgaatctg cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat
    16441 gcttgttcat gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt
    16501 aaaaagggtc atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta
    16561 agcctgctcc atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact
    16621 gaatggagct attgacttgt ccacgtgctt atcgatagcc gtttatcggt ttcttcattc
    16681 ttatgataga ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga
    16741 tcacacagat ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg
    16801 aaacatcgaa acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta
    16861 agtttactaa accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg
    16921 caaattcacc cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag
    16981 gaatcaagaa tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag
    17041 ataatgaact taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt
    17101 tcataaccac ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag
    17161 aagcaccagc taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta
    17221 ctacgatgga cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg
    17281 gaagcatgct tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca
    17341 atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca
    17401 tgataccaaa agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt
    17461 cttgacgacc gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata
    17521 ctaatgattt tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag
    17581 aaatagctaa caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa
    17641 tcccaacagc ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc
    17701 ctacttgaga ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca
    17761 tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag
    17821 agccgcctaa tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga
    17881 atttcttcac aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta
    17941 aaaatccacc aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag
    18001 ttagctcccc cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca
    18061 gcccttgagg attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt
    18121 ctacactata gtgcccagct ggtataaacc aagtaagtac agcgattact gcaataataa
    18181 taaataagac cgtatacgct gaaggcattt gaaatttttt cttttttgtt tgttccatct
    18241 tcttcttcct ttcttttccg ttagagacaa caattttggc tctaatgtta ttttatcaaa
    18301 aagagtatca gaacaaaaat aataacacgc attttgtaaa acttttgttc aaaaagttcg
    18361 tttttcccta ttccctcctc ttttttcagt tttttaaagc tttcttcttt ttcattgtag
    18421 cgtttccttt tttttataaa caaacatttt cgtcttttaa aaggagaaga atttcacatt
    18481 tgcaaagaat tgattgattt ttatgcataa atcaaaaaaa tactcaaaa
//
LOCUS       DAZUKZ010000046        18151 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000046 DAZUKZ010000000
VERSION     DAZUKZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.046"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..152
                     /locus_tag="ACG8EF_001538"
     CDS             <1..152
                     /locus_tag="ACG8EF_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4946851.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="ACG8EF_001539"
     CDS             complement(230..763)
                     /locus_tag="ACG8EF_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFS4946852.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ACG8EF_001540"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ACG8EF_001540"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFS4946853.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ACG8EF_001541"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ACG8EF_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFS4946854.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="ACG8EF_001542"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="ACG8EF_001542"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS4946855.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="ACG8EF_001543"
     CDS             complement(4488..5066)
                     /locus_tag="ACG8EF_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFS4946856.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="ACG8EF_001544"
     CDS             5388..5858
                     /locus_tag="ACG8EF_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFS4946857.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="ACG8EF_001545"
     CDS             complement(5966..7192)
                     /locus_tag="ACG8EF_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4946858.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="ACG8EF_001546"
     CDS             complement(7946..8146)
                     /locus_tag="ACG8EF_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4946859.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="ACG8EF_001547"
     CDS             complement(8280..8888)
                     /locus_tag="ACG8EF_001547"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFS4946860.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="ACG8EF_001548"
     CDS             complement(8973..9443)
                     /locus_tag="ACG8EF_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFS4946861.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="ACG8EF_001549"
     CDS             complement(9491..10405)
                     /locus_tag="ACG8EF_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4946862.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="ACG8EF_001550"
     CDS             10551..11354
                     /locus_tag="ACG8EF_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFS4946863.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="ACG8EF_001551"
     CDS             11532..12479
                     /locus_tag="ACG8EF_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFS4946864.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="ACG8EF_001552"
     CDS             complement(12547..12840)
                     /locus_tag="ACG8EF_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFS4946865.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="ACG8EF_001553"
     CDS             complement(12868..13533)
                     /locus_tag="ACG8EF_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFS4946866.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="ACG8EF_001554"
     CDS             complement(13673..14305)
                     /locus_tag="ACG8EF_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4946867.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /gene="liaS"
                     /locus_tag="ACG8EF_001555"
     CDS             complement(14312..15379)
                     /gene="liaS"
                     /locus_tag="ACG8EF_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFS4946868.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /gene="liaF"
                     /locus_tag="ACG8EF_001556"
     CDS             complement(15376..16107)
                     /gene="liaF"
                     /locus_tag="ACG8EF_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFS4946869.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="ACG8EF_001557"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="ACG8EF_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFS4946870.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="ACG8EF_001558"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="ACG8EF_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFS4946871.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         5383 a         3682 c         2904 g         6182 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat t
//
LOCUS       DAZUKZ010000047        17130 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000047 DAZUKZ010000000
VERSION     DAZUKZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.047"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..598)
                     /locus_tag="ACG8EF_001559"
     CDS             complement(<1..598)
                     /locus_tag="ACG8EF_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFS4946872.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKL"
     gene            871..3342
                     /locus_tag="ACG8EF_001560"
     CDS             871..3342
                     /locus_tag="ACG8EF_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4946873.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3445..5181)
                     /locus_tag="ACG8EF_001561"
     CDS             complement(3445..5181)
                     /locus_tag="ACG8EF_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4946874.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5178..5882)
                     /locus_tag="ACG8EF_001562"
     CDS             complement(5178..5882)
                     /locus_tag="ACG8EF_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4946875.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6013..6516
                     /locus_tag="ACG8EF_001563"
     CDS             6013..6516
                     /locus_tag="ACG8EF_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFS4946876.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6476..6814
                     /locus_tag="ACG8EF_001564"
     CDS             6476..6814
                     /locus_tag="ACG8EF_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFS4946877.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6835..8253
                     /gene="ffh"
                     /locus_tag="ACG8EF_001565"
     CDS             6835..8253
                     /gene="ffh"
                     /locus_tag="ACG8EF_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFS4946878.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8365..8943
                     /locus_tag="ACG8EF_001566"
     CDS             8365..8943
                     /locus_tag="ACG8EF_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFS4946879.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8947..9468
                     /locus_tag="ACG8EF_001567"
     CDS             8947..9468
                     /locus_tag="ACG8EF_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFS4946880.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9547..10101)
                     /locus_tag="ACG8EF_001568"
     CDS             complement(9547..10101)
                     /locus_tag="ACG8EF_001568"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS4946881.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10354..10629
                     /gene="rpsP"
                     /locus_tag="ACG8EF_001569"
     CDS             10354..10629
                     /gene="rpsP"
                     /locus_tag="ACG8EF_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFS4946882.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10641..10892
                     /locus_tag="ACG8EF_001570"
     CDS             10641..10892
                     /locus_tag="ACG8EF_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFS4946883.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11017..11808
                     /locus_tag="ACG8EF_001571"
     CDS             11017..11808
                     /locus_tag="ACG8EF_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946884.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11978..12499
                     /gene="rimM"
                     /locus_tag="ACG8EF_001572"
     CDS             11978..12499
                     /gene="rimM"
                     /locus_tag="ACG8EF_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFS4946885.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12489..13250
                     /gene="trmD"
                     /locus_tag="ACG8EF_001573"
     CDS             12489..13250
                     /gene="trmD"
                     /locus_tag="ACG8EF_001573"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFS4946886.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13386..13733
                     /gene="rplS"
                     /locus_tag="ACG8EF_001574"
     CDS             13386..13733
                     /gene="rplS"
                     /locus_tag="ACG8EF_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFS4946887.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13865..15001)
                     /locus_tag="ACG8EF_001575"
     CDS             complement(13865..15001)
                     /locus_tag="ACG8EF_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4946888.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(15117..16031)
                     /locus_tag="ACG8EF_001576"
     CDS             complement(15117..16031)
                     /locus_tag="ACG8EF_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFS4946889.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(16122..16550)
                     /locus_tag="ACG8EF_001577"
     CDS             complement(16122..16550)
                     /locus_tag="ACG8EF_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS4946890.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(16555..16929)
                     /locus_tag="ACG8EF_001578"
     CDS             complement(16555..16929)
                     /locus_tag="ACG8EF_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4946891.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
BASE COUNT         5505 a         3100 c         3357 g         5168 t
ORIGIN      
        1 ttagtttgta aagtctgcga tcttcaagga tcgtaaatag ctgattggtt gtcccatcaa
       61 cacaaataaa actcatggaa ccagaaacag aattcattga tttgaactca tccatacaca
      121 acaccgtcgg aaggaagttc atacgtggct ggtaatgctt cgttgcttca cgcaagatcc
      181 gtgtcaccgt cccatccgag acgaagtact tcttagcgat tagtttacgt gattgatttt
      241 cgtacagttc catgagaatc acctgcttca gttcctttgt tagttgatga tgttcatcaa
      301 ctaagtccgt tttcgcagaa aaggtccttc cacaatcatg gcagagaaaa cgggtacgtt
      361 ttaattctaa aatagttaac ttttctttga ctttcaacat ctgagtgcgt gtcgtatagg
      421 aaccgttgcg gataatacga accgatcgac agtgttcaca ataggaagaa gagtcatcca
      481 attttccttt aataaactgc gcttgaacgc ctttatattc tttttcaatc agccattctt
      541 catcaaaaaa taaatgttta tctgttaatc cgagtaattt tctagtatga ttatccattg
      601 agagactcct ttggtaggat gacgttttgg tcgactaaat tttaccaaaa gtgagtctct
      661 ttttgagttg aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt tccaccaaca
      721 caaaatatta tagaaccctt tttttttgct atatgacagg aatgaaaggt gtttgcaagc
      781 ttaatgtgag gaacactttc catatctgtt ctatactgga taggagaagt ttggaaatat
      841 ccagaaaacg aaatcaatcg aggtagccac atgaagaata aatggataaa tcgaagtaaa
      901 aaaaagaaag taacacaaag atcatcagat aaagtcatcc gtctttttcc atcgtataaa
      961 gaaggattaa cggcagatca agtcaaggaa cgaatagaaa aaggtgcagc aaataattca
     1021 gtcgatccca cattcaaaac aaatcagcaa atcgtattgg aaaacatatt tacgtatttc
     1081 aatttgatct ttcttatctt agccattttg ctttgtttag tagaatccta taagaacttg
     1141 acttttttac cagttatcat agccaataca ggaatcgcca tttttcagga aattcgatcc
     1201 aaaaagatct tggacgaact aaatgtttta aatacagcaa aagtcaatgt catacgagac
     1261 gggatagaac aaacaataga tatagaagac cttgtaatag atgacattgt tattttagag
     1321 actggatatc aaattccagc agatgcagag gtcgtggaag ggaaactgca agttaatgag
     1381 gctttattga caggcgaagc agatgaaata accaaagaaa taggtgatcc attaatgtcc
     1441 ggcagtttca ttgtttccgg aaaagcttac gcccgattag acaaagttgg agcagattcg
     1501 tatatttccc agctgacagt caaagccaag acaatgggag aaggagaaca gtcggagatg
     1561 attgcttctc ttaatcaatt gatcaaatgg attgggatca tcattattcc tatcggtatc
     1621 ctgctttttt ctcaaagtta cttttttaat ggaaacacga tcaaagaaag tgtcgttgca
     1681 atggaagcag cattgatcgg tatgatacct gaaggactgt acttgctgac gacgattgca
     1741 ttagcattaa gtgcgactaa actagcaaag caaagagttc ttttacacaa tatgaaaagc
     1801 attgagacat tggcaagggt aaatgtcctt tgtgtagata aaacaggaac gatcacagaa
     1861 aataaaatgt ctgttcaaaa aatgatcgtc tctaaaaaac aagaaataat cagtcagtca
     1921 gatcaattgg aagcatgcat tgctgactat gcaaaagcga tggcaggaga caatgcaaca
     1981 atggaagctg ttaaagcata ttttacaaaa accactgatc aatcctatca ccatgttatt
     2041 cctttttctt ctgtccaaaa attcagcagt gtagcgttgg cagacaaggt gtatgtttta
     2101 ggagcgcccg aaatggtttt gcgcgatcaa ttgccggaat atgcggaaga gtttgtttct
     2161 ttcgctgaaa aaggctatcg cgtgttggtc tttggtgtgt acgatgggat tcttgaagaa
     2221 ccagtattga cagaagcagt cacaccatta ggctatattc tgattgctaa tcctatcaga
     2281 aaagaagcaa gagccacatt tgattacttc aaaaaacagc aagtagccat caaagtgatt
     2341 tctggagata atccgctcac cgtatccaat gtagctgtcc aagcagggat ttcacatgct
     2401 gatcagtacg ttgatgcatc taaattatca gaagaagaat atgaagcagc tgtcgaaaaa
     2461 tatacagttt ttggacgcgt caagccagaa caaaagaaaa tcttcgttca attgataaag
     2521 cgtaacaata cagtagctat gacaggtgat ggcgtgaatg atattctagc catgaaagaa
     2581 gcagactgca gtatagcgat ggcttccgga aatgaagccg ccatgcaggc ctcacaagtg
     2641 gtcttacttg attctgactt ttcacgcatg ccagaagttg ttgcagaagg acggcgcgtt
     2701 gtgaacaata ttgagcgatc agccagcttg tttttagtga agaatatttt ctcctttttg
     2761 ctctcactgt tttcagtgat ctttgctctg acctatcctt tagaaccatc acaaatcaca
     2821 ttgattagtt tgtttactat cggacttcca tctttcttgc ttgctctaga agaaaacaaa
     2881 aagcgaatca gagggaaatt catcatgaat gtgatggaaa aagctgtgcc aggtggacta
     2941 acagatatga tcgtagtcgg tgcgttggtg atctgcgggg tgactttgaa tctaaataaa
     3001 acagatgttt ctacagcatc taccatgctt ttgattgcag tagggttcct cgttctctat
     3061 aaaatctgtt caccgctgaa taaattcaga agtcagatca ttcttttctg tgctagcggt
     3121 aacttttttt cggtcatctt tttgcataag ctgttttcaa tcaccggtat ctcagctgta
     3181 tcacttttgc tgttgagcat tctatttttc tcggcagatt cgatttttag gcatttgacc
     3241 acattggtag aatatttgtt tgaacgtaaa aatgaaaaag agcaaaagcc gtttagtcgc
     3301 gtagctaaaa aatttctatc gatatttaaa cgagaaaatt gacgaaaagt atctcaaaaa
     3361 agagtaaaaa agaacaacca ctcgtgtatc tttcgaagca ggatgagtca tccaacttta
     3421 aaacgcacga gcagttgctc ttttttacat tttcggaatg gtaacggtga aagtcgaacc
     3481 tgttccaaga tgactgtcta cagtaatcgt accattaagg agttgcacgt agtcttttac
     3541 aatcgctaac cctaatccgg tcccgcctga atgtcggctt cgcgccttgt ctacgcggta
     3601 aaaacgctca aagatccgtt cctgatcctc aatgtcgata cctataccaa agtcttggac
     3661 agaaaacgat agatcatctg ttgcctgata gcggataata atctcgctgt cgctttttga
     3721 atattggaca gcattttcaa ttagattttt gataattgga taaaggatat ctgttttagt
     3781 agtaaatggt tcgttctttc caatcaatcg aatcgtcaac cgtttctttt cgatgattgg
     3841 ctgataattt tgaacgattt gctggaaata aggttctgta taaattgtct ggatctcgta
     3901 agaattctcg ccatcttttg acagctggat gatttcccga atcagtttat ctaaccggat
     3961 cgcatctttt tgcatgatct caagaaaaga agtcagagtt tgcgggtcat cttttgcacc
     4021 atccagtaaa gtctcggtaa aaccaatcag tgaagtcaca ggagttttta attcgtgaga
     4081 gacgttgcca acgaaatcct tttgtagctt ttctaaacgt cgcactttcg ttaagtcata
     4141 cgccacccct agaatctgtc cggtaccttc tgaattagta aagtagcgca aactcaagtc
     4201 taatgtctgc cgaccttctg taagggtgat ctcctgatgg atcagcggat gctcaggagt
     4261 cacttgatga atcagctgaa tgagttttgg ctcttgaatg acttctgtgt accgctgttc
     4321 aggttgatag cttaccacgt ttaaatgtag ctgcatcttt ggattaagca gtagtaaacg
     4381 agaatcagca tcatcaatca ggaatacgcc aatcatcagt tcgttcagta acgtatataa
     4441 ttgctcttct gtggtagtac acgcccgata catcttactc atttgttcgc tcaacgcatt
     4501 aatcgtctga tacaattctt cccattgatc gggtgtttgc atgattactt ccgcctgatt
     4561 aggattttgc accatttttt ttagtacggg cagcacggtt ttcaatggct ggtttttttg
     4621 atagatcaga taatagatca ttcctgcaag aacaaggaag aaagacagaa agaagaaaaa
     4681 gacccaacgg cgaaaactct cagttctcgg taaaaaaccg cttgtcggtt ctgcgatacg
     4741 taaaatggct tctaactggc cacttttttt gactggtaat gcgacatata aaagatcctc
     4801 attcaaagta gtacttttgc gcaaagcgct acctaggttc ccaccattca atacggcttt
     4861 gatttcggga cgggagtttc gctgttcatt caatgtttca ttgtttgtgt caaataagat
     4921 ctttccagta gcatccatta aagtaatccg ttcgttcgac tgatggacga attcttccaa
     4981 agcagttttg ttttgcgctg cttggaggtt ttcgacatct aattgccgga tcagcagttg
     5041 tgcttttttt gtcaggtagc tttcttgctg ttccacgatc tgctgttgaa aataatggga
     5101 aatcaattgc cagccaccaa aaaacaaact aactaaaaga ataagaaaga agagacgttc
     5161 ataaattttt ttccatttca tcgtttgggt tcctgaaact tatagccaaa tcctctaata
     5221 gtcaccaagt actttgggtg ttttggatct cgctcgattt tttctcgcaa atgactaacg
     5281 tgtacatcta cgattcggct ttgtcctgcg aagtcaaaat tccaaatgcg gtctagcaat
     5341 gtgtctcggt cgatcacacg gtcttttcgt ttcatgaaat aaacaagtaa ttcaaattct
     5401 ttcggtgtta gttcaatcag atggtcgtct acggtcactt gataattggt caaatcagca
     5461 gtgatttgtc caatcgatag atattcaggc tcagcttcat cttttgattg cttacgaggt
     5521 tcaattcgtc tgaatattgc tttcatacgg gctaatactt cgcgaggact aaatggtttg
     5581 gtcaaatagt cgtcagcacc tatttctagg ccaatgatcc gatccacttg gtcatcttta
     5641 gccgtcagca tcaaaatcgg tgtttcattt ttttcttgac gcagccgctg agtgatctcc
     5701 atgccatcca tcgttggaag catcacatct aaaatgatga aatcaaatga ttgttctaat
     5761 gctaattcta atccttcgcc tccatctgtg gcactggtta ccttgtagcc ttctttttcc
     5821 aaattgaacg tcaagagcgt tacaatagaa ggttcatcat caacaactag tatttttttc
     5881 attcaaatct cctttaaata ggtttcattc tgtttgtatt ttagcataga atcagtacaa
     5941 agggctattt tacaaaagaa ataaatggta taataggaag gcactttgct gaatagaaag
     6001 gaagagcagt caatgattgg aatcagtgca tgtttaggcg gtctggcctg tcgctatgat
     6061 ggacagaaaa aagaagtacc agaattgatg gaactagttg aaactggaga agctgtcatg
     6121 gtctgtccag aagtgatcgg cggattacca attcctagag atcccgctga aatcattggt
     6181 ggaaatggat tcgatgtgtg gaaagatcaa gcaaaagtat ggacgacaag cggcgaagat
     6241 gtaacggaag cgtacaaaaa tggcgcgatc aaagcctatc aaaaattgca ggaaaaacac
     6301 attgatctag tcatcatgaa ggaaaaaagc ccttcatgcg gaacccattc gatttatgac
     6361 ggaagttttt ccggaataaa aaaaaatggt gccggagtgg ctactgctta ttttattcag
     6421 caaaaaatga ctgtgctctc tgatgaagaa tggctgaaaa ctagaggtga gcagaatgga
     6481 aatcgaaaag accaatcgaa tgaacgcgct ctttgaattt tattcgacct tattgacaga
     6541 aaaacaaatg aactacatgg aattatatta tgcagatgat ttttcattag gtgagatcgc
     6601 ggaagaatac gaagtaagcc gacaagcagt atatgacaat atcaagcgga caagcaagat
     6661 cttggaggat tatgaaaaaa aactccatct tttttctaat tatattgttc gagaacaggt
     6721 attggaaaat atgactgcat atattacaga taagtacccg gaagataaaa aactacttga
     6781 atatgtacag caaattcaag caatcgaaga ataaataaaa ggaatgaaga aaatatggca
     6841 tttgaaagct taactgaacg tctccagcag gcgatgggaa aaataagaaa aaaaggaaaa
     6901 gtatcagaag ctgacgtaaa agaaatgatg cgggaaatcc gccttgcctt actagaagcc
     6961 gatgttaact tgcaagtagt caaagatttt actaagcgcg tacgcgaacg tgcggtaggg
     7021 gcagaagtgc tggaaagttt atcacctgca caacaaatcg taaaaatcgt tgatgaagaa
     7081 ttgacgatca cacttggttc tgaaacagca gagttaaata aatcaccaaa aataccaact
     7141 gttatcatga tggctggtct tcaaggggca ggtaaaacga cctttactgg taaattagcc
     7201 aattatttaa agaaaaatga aaatgctcgt ccgctcctaa tcgcagggga cgtatatcga
     7261 ccagctgcga tcgaccaatt gaaagtatta ggtcaacagc tagacgttcc tgtatttgat
     7321 atgggaactg aagtcagccc ggtagagatc gtccgtcaag ggatggagtt agcaaaagaa
     7381 aagaaaaacg actatgtatt gatcgatacg gcaggacgtc tgcatatcga tgaaacattg
     7441 atggatgagt tgaaacaaat caaagaattg acgcaaccaa atgagatttt gcttgttgtc
     7501 gatgccatga ctggtcaaga tgccgtcaac gtagctgata gcttcaacca gcaattaggt
     7561 attacagggg tcgtcatcac taaacttgat ggggatactc gaggcggggc agcactatcg
     7621 atccgctcag tgacaggcgc accaatcaaa ttcatcggtt ctggtgaaaa attgactgac
     7681 ttagaagtat tccacccaga tcgtatggct agccgtatcc ttggtatggg ggatatgctg
     7741 acattgattg aaaaagcgca gcaagactac gacgagaaaa aagcggaaga acttgcacag
     7801 aaaatgcggg aaaattcatt cgatttcaat gactttattg aacagcttga tcaagtgatg
     7861 ggtatgggcc cactggaaga tctaatcaaa atgatcccag gaatgaatca agtccctgga
     7921 atcgaaaacg tcaaggtaga tccaaaagat gttgaacgca aaaaagcgat ggtttattct
     7981 atgacgccag cagaaagagc aaatcctgat ctgttgaacc caagccgtcg tcgccgaatt
     8041 gctgccggtt ctggaaacag tgttgttgaa gtgaatcgaa tgatcaaaca attcaaggaa
     8101 tcaagaaaaa tgatgcagca aatgtccaaa ggggatatga acatcccagg tatggaccaa
     8161 atgtttggtt caggcgtcaa aggaaaacta ggcaaaatgg cgatgaatcg aatgatcaag
     8221 aaaaataaga agaaaaagaa aaaacggaaa taagatgaaa aaaacagctt cggtacagac
     8281 tgaaaatgtc tactgccgga gcttttctat cagcaatttt tattctttac taatacaatt
     8341 actctaaacg aagaggtggc acagatgaga tgtccgtgga gtgaacaaac agaaagtatg
     8401 cggcaatatc atgatactgt ctggggtgtt cctgaatatg atgaccaaaa gctgttccga
     8461 aaattgatgc ttgatattaa tcaagccgga ttgagctggc agacgatttt aaataaaagt
     8521 gaagcattcg atgaagcata tgatcgattc gatatcaaaa aagtcgcaaa ttatggagaa
     8581 acaaaaatca gtgaactaat ggaaaacaaa ggaattattc gtaaccgaag aaaaatcgaa
     8641 gcagccgtcc aaaacgccaa agcagtcttg aaaattcaag aagaaatcgg cagtttttca
     8701 gagtatcttt ggtcgttttc taatggaaaa atcgttgatt cttcttatca ttcacaagaa
     8761 gaagtcccaa caacaagtcc tctttcagat gaattagcaa aagatctaaa gaaaagagga
     8821 tttaaattta tcggaagcac aacaatctat gcttttttag aagctgtcgg gatcatcaat
     8881 gatcaccttg acagttgttt ccgcaaagaa gaggtcgtac gtatcggcag aaagggcgaa
     8941 taaagcatgc gaaaaatcat attttatggt gctataagtt tagatggcta tttggcaacg
     9001 aaaacggatg atcttcaatg gctttttgac actccaacag gtaagcaaac tacttatgaa
     9061 gctttttatg aaacaatcga tacgaccatc atgggcagaa aaacttatca ggaagcaaaa
     9121 aaatatttgg agaccgaagc aatttatcca gagaaaaaga atttcgtttt ttccacccac
     9181 gcacaattac aattgccaga tgctgaggtc gtaaacgaag atcctgttgc ttttctaaaa
     9241 aatctacaaa tgacagaagg aaaagatatc tgggttgtgg gtggcggtcg attgatcaaa
     9301 ccgctattgg aacatcaaat gattgatgaa tggtatatcc agattacccc tgtattatta
     9361 ggggacggga ttcctttatt ccaaaaagga acatatgaag cgcgttttga aatgctagac
     9421 acgacacgat tcggtgaatt tatcgaattg cattatgtac gaaagtagaa gtaaataaaa
     9481 taaaaaaggc aactatatgc agaaatgtcc tgtacatagt tgcctttttt attaccgtta
     9541 tgctttttac tctgttaatt tgttagaaaa atctagatac gcatctaaaa tcgtttggaa
     9601 gaaacttaat gttccttcaa cgatattacc attttcatca attagattcg caacgtttcc
     9661 gatatatgct tccggttgct gcaaggttgg cacattcaaa aagacaagtg attggcgtag
     9721 atggtggttt gcgccaaagc cgccgatccc acctggtgat gcagtaacga ctaaacctgg
     9781 tttatgatcc caaacactgt gtccataagg tcttgagcct acatctaaag catttttcaa
     9841 tactgctggc acagaacggt tgtattcagg agtaacgaaa acgacaccgt ctagtcccct
     9901 cacttcctca cgaaaacgag tccattcagc aggagcctgt tctggagttt cgagatcctc
     9961 attgtaaaat ggcagatcat cgatttttac aaatacactt tcgtaccctt caggaagcaa
    10021 tttcgcaaaa gcttctgcta cttttttgtt gtaagaatct tttcttaaac taccaataaa
    10081 aaatccaatt ttttttgtca tacgattttt cctccttcaa actatctata ataatcttac
    10141 aaaagataag taactggagc aaataaaaac acttactgac aaaagctaca tagaagatag
    10201 attacttatt tttaacaaaa aagaagtcta aaatcttcta ttttctaaaa aaaatcgaat
    10261 ttaatcaagc tgtaaagaaa aaaagcttta cagggtaaaa aaaaactgtt acactacatc
    10321 ttgtgaataa aacaatggag gtggaaataa ataatggcag ttaaaatccg tttaaaacgt
    10381 atgggttcta aaaagagtcc gttttaccgt atcgtcgtag ctgattcacg ttctcctcgt
    10441 gatggacgtt tcatcgaaac tgtaggtact tacaatcctt tgaaagaccc tgcagaagta
    10501 gttttaaaag aagacttagt tcttgactgg ttatcaaaag gtgcacaacc ttctgacact
    10561 gtacgtaaca tcctttcaaa agaaggcgtt atgcaaaaac accacgaagc taaattctca
    10621 aagaaataag gtgaccggat atgaaagatt taagcgactt agtcttaaca atcgttcgtc
    10681 cgctagtcac ttatcctgat caagtcaagt tagacgtcgt agagtcgaaa gacttttacg
    10741 agtacaatct aacagtggct cctgaagata ttggccgtat cattggaaaa caagggcgtg
    10801 ttgctaaagc aatccgcaca atcgtttacg gtgttcggat caatgcaccg aaaaaagtgc
    10861 gtttgaatat catcgatgat aaggaaaagt aaataaacgt gctgtactcg aattttcgag
    10921 tgcagctttt ttgttttttt tattaattga agaaatgatt atttggcaaa acaagcaaga
    10981 aagcgtatcc ttataggagg agggagtgaa cagccaatga gaaagaaaat gttatttatt
    11041 ccatttgctc tattgttaag cgcttgtagt tcggttactc aagaatcaga caatacacat
    11101 ctatcaagca gtatatctga aactgttacc agtccagaaa aaagtagtac caaaacggct
    11161 tccaccactg aacaaacaat ggctacctca aataatgaga aaaagactgc tttagaccag
    11221 ttgaaagaac aacagccaaa tgttcccatg cctctagatg taccagtctc ttcaggctat
    11281 ctcaatatcg cagcgacaca tacaaagcaa ggttattcta ttttatatta cagaacagat
    11341 cgtcccctcg ggttgaatgc tgacgaattg aatcaagaaa cgcctattgc aacctatcta
    11401 tatcaatatg ggtttgcttc tagtcaagaa acaatccaag ttttgcagcc atttgagatc
    11461 gacacaaatg gtcagcaagt ggacttaggt tctcgaatca cagggtatca gcaaggtgca
    11521 gcaggttcaa gttttcttga atggcaagaa ggaaactggc gtattcgaat ccgtgggaat
    11581 aatatagaag gccaagatcc cttactatta gctaaagaaa tcgtagctta tttggaagaa
    11641 aacagtctac cagcacctga acaatttggt aaaattactg ttgatatggg tgatacgacg
    11701 aatcgagccg tagaagtaag ctggcaagaa ccgaaaaatg cctatacgat cactcatcaa
    11761 gatcctatgt ctgcgttgaa gatggcagtt tctatgaaga ggttgtgaaa tcagcgcgct
    11821 gatctgagcc catcatttaa ctccaagtca caggaagtaa tcaaaaatag cacgccgcat
    11881 ttttgcataa ttttggctta ataccttaaa agaaaactag aattgcctct tcacacatgc
    11941 taaaataaaa cagacaaaaa agaaatgggg atataagttg actgaatact tgaatgtagg
    12001 aaaaatcgta aacacacaag gtatcaaagg cgaagtccgt gttatctcaa caacggattt
    12061 cccagaagaa cgttacaaaa aaggaacggt gctaacacta ttccaagacg gaaaagcacc
    12121 tgtggaatta acagtcaaaa gtcatcgcaa acataaaaat tttgacttat tgagttttga
    12181 aggacatcct tctatcaatg atgtggaaaa atatcgggat ggcatattaa aagtgtcaaa
    12241 agacaactct gtagatctag ctgaaaatga attctattac catgagatca tcggtctgca
    12301 tgtattagaa gatggaactg agttaggtaa agtaaaagaa atcttgtcac caggtgccaa
    12361 tgatgtttgg gtcgttcaac gtccgaaaaa agctgatata ttgcttccat atatcgcttc
    12421 tgtcgtcaaa gaagtcgatt tagaagctgg agtggtccgt gtagaaattc cagaaggact
    12481 gattgacgat gaagattgat gtattgactt tatttccaag aatgtttgaa ggtcctatgg
    12541 gggaatccat catcggtaag gctgtagata aaggattatt agatatcaat atctctaact
    12601 tccgcgatta ttcagataac aaacatcaga cagtagatga ttatccatac ggcggtggag
    12661 caggtatgct gctcaaagtc cagcccatct atgataacat ccaagccatt gaaaaagctg
    12721 caccggatgt caaaaaaaga gtaattcttc ttgacccagc tggtaaacga ttcgatcaga
    12781 aaatggcaga agaattttca caagaagaac acttagtatt catttgtggt cattatgagg
    12841 gatatgatga acgtatccgc acattagtga cagatgaagt ttccttagga gattatgtcc
    12901 ttactggggg agaattaggt gcgatggtca tgatcgatgc aacagttcgg cttttgccag
    12961 atgtgttagg gaatcagact tcagctcaaa cggattctta ttcaaccggt ttattggaac
    13021 atccccaata tactcgcccg gcagaatata aagggatgaa agtaccagaa gtattgacaa
    13081 atggaaatca taaattgatt gaagaatggc aattaaaaga atcactaaga aggacctatc
    13141 aacgtcggcc tgatcttttg gaaacacttg aatggacacc tcaaatgacg aaatttttag
    13201 aagaaatcaa gaaagaagaa caagaaaaaa cagcagatga agccaagtga ctttttctaa
    13261 aatcttcttt acagtaggtt gataatatgt tagtatttta tggtgagtgc aaaagcactt
    13321 aactattacg atattccgct gtgagaaaag ctcataagaa tatttggaat aaggagaaga
    13381 aaaccatgaa tccattaatc gaagaattaa caaaagaaca attacgttct gacatcccag
    13441 cgttccgccc tggtgacact gtgcgtgttc acgcaaaagt tgttgaaggt actcgtgaac
    13501 gcatccagtt atttgaaggt gttgttatca aacgccgcgg tgctggaatc agcgaaactt
    13561 atacagtacg taaagtttct aacggagtgg gcgttgaacg tacattccca ttgcatacac
    13621 cacgtgttgc aaaaatcgaa gtagttcgct acggtaaagt acgtcgtgca aaattgtact
    13681 acctacgtgc attacacgga aaagctgctc gtatcaaaga aatccgtcgt taatcatcgt
    13741 tatcagactg agaaagaacc cttggtattc aagggttctt tttttgttct ctgttttttt
    13801 atattaacga tttctatttg ggacattttt gattaataaa aaggggcaaa aaaggggcaa
    13861 caagttacag tttttctata tcttctttca atttttcttt tactcccttt gtcacatgca
    13921 aataaatctt ttcagtgata tcactatttt cgtgcccaac gcgatcctga atagcataca
    13981 gaggagttcc tagttccgct aattttgaaa tatgggtatg tctaaaaata tgtgaactaa
    14041 gttttttatc aatcttcatg tcagctttat gatttctcaa ataggtgtta attgctgtta
    14101 gttggaaagg agttcctttc gttgtttgaa atagaaattg accattcgga tttaattcta
    14161 gaagctcatt gtaaatagct atcgcttttt ttggtaaatc aatttctcgc attccagcag
    14221 cagtttttgt agaatcagat tttttcatat cagctattga acgttctcta tacatcatcg
    14281 tcccatttat aactactgat gcattattat tggtaatgtg tacatcgtct ttacttaatg
    14341 cgattgcttc tccaggcctc attccagtca aatatagcca ctggaaaagg agagaatatc
    14401 ttttattgtg tgaagttgtg aaatttacta gtcttttata ttcatcatct tctaaaaact
    14461 tatctttaat ttttatcgtt tttgactctc gtttataatc aataactact tcgttgatag
    14521 gatttttttc tacatagcct tttttcatag cgtatgaaaa aagaagattc aatttggatt
    14581 taattacact gacgtactta tttgacaagt catctttata tatcatatct tcaaaaatat
    14641 tatttaaatc tactgtatta ataccagaaa cgatgtaagt ctcaggtatc tttttcttta
    14701 tagtgtttaa gatattattc gtaggataat atgtagactc cttaacttgt cttttgtaaa
    14761 taactaacca ttcttcgaca agctcatgaa atgttagatc aggcttctga agtgtttttt
    14821 cgtttaattt tatatcaatt tttttattta actctagcaa tgccactttc tgagtttctc
    14881 gagatttatt tttataagta atgctcactt tttttctttt tctagttttt ggatcaatat
    14941 atcgctcatt atatttatat actttttttc cgtttttatc agttttggtt tcaatccaca
    15001 ttgttttcat ctcctatttg ttgctataat aggaatggat aagtaagcca attatagcag
    15061 gtttattttt catcacgtcc acaaactttg gcgagggagg gggcgtgttt ttttgtttag
    15121 tagctacctt tccatgaagt atctgcgaag aacacttcaa atgttgttcc atcatttttc
    15181 acttcaaaat atgctgttcc ttgtgcggat ttacccggtt ggatggtttc cgaaaagtaa
    15241 tctctactag ctaaatcaga tttaatatct gatgaatcat aaaattctaa ataacttgaa
    15301 cttgcatcaa aaggtgtagt ccctgtgttt tctacaagaa aatctacctt tgcgtaaaat
    15361 aggccttctg gtttatagaa atcatcaccg taatcttttg atacagactg gacagttacg
    15421 ttaatagagt taccattttc agaagtaaaa ggaactgctt ctcctatact atgtgcaact
    15481 gatgccgtct ggggttctga agacgaagtt gtagttgtag ttgtagtaga gcttgaatct
    15541 ttggattttc catctttatc taagaaagtt cgttgattat aatatacaac ctgttcagtt
    15601 tttggactaa cccaaattaa cattgtttca tcatcagaat cagttgaata agtatattgg
    15661 agcatctcta atttttttat atcttttgca ctatttccat gttcaattaa ttcagagtgc
    15721 tctttataat aatctccttg tgcatccttt gaaatagata caattaattc atcccgtgtg
    15781 atagcattgt ctaattcttt gtaaacaaaa tcatttgtcc atttacgtaa cggtgaacca
    15841 agtgtctgat aaacttgagt taaatcatca ccaactttta ttttatgaac gtctgatgaa
    15901 gttagttttt ttgtttcata ttcagatgtt gatgaaacag tattttcttt agtgctagca
    15961 gaattaggac tagtggagca tccgcttaat accaagccta aaaaaagcaa cccaaaaaca
    16021 atttttttca tttttaattc ctcacttcta tgatatgatt tttttgtagg atcttagaaa
    16081 taaggttttt agtccgtgtt gcagcacgga ctttttttac attatataag aaagcgataa
    16141 gcgctttctg gaagtccgta aagattcttt aattcctcaa ttcgtttagg atattgatca
    16201 ttgtcttctt tgtatagaga aacaatgaga ttagcagcaa agcaattagc ttcgctttca
    16261 gatttgcttc tagatgttct tgttgataca tagtaactgg ataagccacg atgaaaaata
    16321 gcgtgaccta attcgtgagc gcaaatgtag aatctttcct cagagtctct cagttcatca
    16381 tttaagaaga ttattgcacg acctctaatt tcttgaaact gtcctttggg attctcgata
    16441 aaaggaacgt attgaatttt aatgcccatc ttttcacaaa tataaaaagg attagcggac
    16501 tggtatttcc gcttcaactc ctcgactaaa ttaatcgtat ccatctccat aagctcacat
    16561 ctttttgcct ttttctttgt cttctttcac aatatcccag aaggttccaa taaggatatc
    16621 ttttacgcgt tggacctgtt caggcgtcag tgtttctccg ccataagaca tattgacatt
    16681 tgaatctagt aatttatcaa gttcaactac ttcatctttt gttgcccagt caggaacttg
    16741 gtttctaccg agtaaataat cagtagttac attaaaataa tctgctacaa gttgtagtct
    16801 tttagtacta ggagttctat ttttccattg ataaatcgtg tttttaggta tatttaattc
    16861 ttcttccaat tgagttatac ttatgcctct tttatgagct aattccttta ttctatctag
    16921 taaattcatt ttcgcacctc acaagctacg aaaacactaa taaaaaaatt agttaaaggt
    16981 gttgacaact aataaaagtg ttagtatact gttttcgtaa gctaaaatat tagctaaaaa
    17041 gacttgataa ctaataaaca cttcacggtc ggcaaactct gaaatgtaaa ttactaggca
    17101 tttctgtgtc ttatttagct atgtctatat
//
LOCUS       DAZUKZ010000048        16578 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000048 DAZUKZ010000000
VERSION     DAZUKZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.048"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..727)
                     /locus_tag="ACG8EF_001579"
     CDS             complement(29..727)
                     /locus_tag="ACG8EF_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFS4946892.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="ACG8EF_001580"
     CDS             complement(720..2072)
                     /locus_tag="ACG8EF_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFS4946893.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="ACG8EF_001581"
     CDS             complement(2295..3695)
                     /locus_tag="ACG8EF_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS4946894.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="ACG8EF_001582"
     CDS             3856..4593
                     /locus_tag="ACG8EF_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFS4946895.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="ACG8EF_001583"
     CDS             4718..5197
                     /locus_tag="ACG8EF_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFS4946896.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="ACG8EF_001584"
     CDS             5194..5961
                     /locus_tag="ACG8EF_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFS4946897.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="ACG8EF_001585"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="ACG8EF_001585"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4946898.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="ACG8EF_001586"
     CDS             complement(6864..7466)
                     /locus_tag="ACG8EF_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS4946899.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..7802
                     /locus_tag="ACG8EF_001587"
     CDS             7572..7802
                     /locus_tag="ACG8EF_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946900.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7793..8041
                     /locus_tag="ACG8EF_001588"
     CDS             7793..8041
                     /locus_tag="ACG8EF_001588"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4946901.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8147..8731
                     /locus_tag="ACG8EF_001589"
     CDS             8147..8731
                     /locus_tag="ACG8EF_001589"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS4946902.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="ACG8EF_001590"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="ACG8EF_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFS4946903.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="ACG8EF_001591"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="ACG8EF_001591"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS4946904.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="ACG8EF_001592"
     CDS             10632..10811
                     /locus_tag="ACG8EF_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946905.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="ACG8EF_001593"
     CDS             10833..11858
                     /locus_tag="ACG8EF_001593"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFS4946906.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /gene="glyA"
                     /locus_tag="ACG8EF_001594"
     CDS             12033..13277
                     /gene="glyA"
                     /locus_tag="ACG8EF_001594"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFS4946907.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="ACG8EF_001595"
     CDS             13430..14377
                     /locus_tag="ACG8EF_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFS4946908.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="ACG8EF_001596"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="ACG8EF_001596"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFS4946909.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="ACG8EF_001597"
     CDS             complement(15279..15521)
                     /locus_tag="ACG8EF_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946910.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="ACG8EF_001598"
     tRNA            15642..15714
                     /locus_tag="ACG8EF_001598"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..16471)
                     /locus_tag="ACG8EF_001599"
     CDS             complement(15833..16471)
                     /locus_tag="ACG8EF_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFS4946911.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5651 a         2952 c         3331 g         4644 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    16261 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    16321 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    16381 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
    16441 gttctatttt ttttcctaaa atgttattca tgataataac tcctactatt cagtttttga
    16501 agatagagtc agtatacatg aagagttaaa ataggcaatg tttttttatg gatatttgta
    16561 ttgctaaaaa tttatctt
//
LOCUS       DAZUKZ010000049        16492 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000049 DAZUKZ010000000
VERSION     DAZUKZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.049"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1452
                     /locus_tag="ACG8EF_001600"
     CDS             <1..1452
                     /locus_tag="ACG8EF_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4946912.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1801..2433
                     /locus_tag="ACG8EF_001601"
     CDS             1801..2433
                     /locus_tag="ACG8EF_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFS4946913.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3021..4460
                     /locus_tag="ACG8EF_001602"
     CDS             3021..4460
                     /locus_tag="ACG8EF_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4946914.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4755..4973
                     /locus_tag="ACG8EF_001603"
                     /pseudo
     CDS             4755..4973
                     /locus_tag="ACG8EF_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            5222..5437
                     /locus_tag="ACG8EF_001604"
     CDS             5222..5437
                     /locus_tag="ACG8EF_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946915.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5654..5910)
                     /locus_tag="ACG8EF_001605"
                     /pseudo
     CDS             complement(5654..5910)
                     /locus_tag="ACG8EF_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6097..7008)
                     /locus_tag="ACG8EF_001606"
     CDS             complement(6097..7008)
                     /locus_tag="ACG8EF_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4946916.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7027..8031)
                     /locus_tag="ACG8EF_001607"
     CDS             complement(7027..8031)
                     /locus_tag="ACG8EF_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS4946917.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8028..10151)
                     /locus_tag="ACG8EF_001608"
     CDS             complement(8028..10151)
                     /locus_tag="ACG8EF_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS4946918.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10156..12603)
                     /locus_tag="ACG8EF_001609"
     CDS             complement(10156..12603)
                     /locus_tag="ACG8EF_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4946919.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12590..12979)
                     /locus_tag="ACG8EF_001610"
     CDS             complement(12590..12979)
                     /locus_tag="ACG8EF_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4946920.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13039..13542)
                     /locus_tag="ACG8EF_001611"
     CDS             complement(13039..13542)
                     /locus_tag="ACG8EF_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS4946921.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13555..13779)
                     /locus_tag="ACG8EF_001612"
     CDS             complement(13555..13779)
                     /locus_tag="ACG8EF_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946922.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13883..15793)
                     /gene="ltrA"
                     /locus_tag="ACG8EF_001613"
     CDS             complement(13883..15793)
                     /gene="ltrA"
                     /locus_tag="ACG8EF_001613"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS4946923.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         4913 a         3271 c         2939 g         5369 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
      301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
      361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
      421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
      481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
      541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
      601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
      661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
      721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
      781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
      841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
      901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
      961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     1021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     1081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     1141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     1201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     1261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     1321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     1381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     1441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     1501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     1561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     1621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     1681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     1741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     1801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     1861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     1921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     1981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     2041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     2101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     2161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     2221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     2281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     2341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     2401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     2461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     2521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     2581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     2641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     2701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     2761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     2821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     2881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     2941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     3001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     3061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     3121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     3181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     3241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     3301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     3361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     3421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     3481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     3541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     3601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     3661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     3721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     3781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     3841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     3901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     3961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     4021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     4081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     4141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     4201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     4261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     4321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     4381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     4441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     4501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     4561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     4621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     4681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     4741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     4801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     4861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     4921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     4981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     5041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     5101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     5161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     5221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     5281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     5341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     5401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     5461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     5521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     5581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     5641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     5701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     5761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     5821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     5881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     5941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     6001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     6061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     6121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     6181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     6241 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     6301 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     6361 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     6421 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     6481 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     6541 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     6601 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     6661 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     6721 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     6781 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     6841 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     6901 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     6961 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
     7021 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
     7081 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
     7141 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
     7201 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
     7261 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
     7321 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
     7381 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
     7441 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
     7501 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
     7561 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
     7621 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
     7681 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
     7741 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
     7801 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
     7861 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
     7921 accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
     7981 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
     8041 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
     8101 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
     8161 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
     8221 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
     8281 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
     8341 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
     8401 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
     8461 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
     8521 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
     8581 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
     8641 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
     8701 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
     8761 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
     8821 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
     8881 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
     8941 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
     9001 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
     9061 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
     9121 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
     9181 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
     9241 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
     9301 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
     9361 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
     9421 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
     9481 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
     9541 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
     9601 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
     9661 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
     9721 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
     9781 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
     9841 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
     9901 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
     9961 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    10021 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    10081 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    10141 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    10201 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    10261 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    10321 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    10381 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    10441 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    10501 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    10561 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    10621 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    10681 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    10741 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    10801 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    10861 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    10921 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    10981 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    11041 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc caccccgttc
    11101 cccttttgga tcgacaatca cagcttgtcc accaaaaagg acggcgtaat aaactaagag
    11161 attgttacta aaggactttc cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt
    11221 ggtaactgaa tccttgaccc cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc
    11281 cgtatctacg ttataaccaa agtaaatgcc ttccaactcc cctaacatct gagtggcgcc
    11341 aaaaccaagt ccggcgagaa aatcagacgt aacatactgg atataatcat tcatgtatcg
    11401 cttactagaa ggtaagaatt cttcatgtag ccccatcata tcgccaaaag gccggactag
    11461 ttttacattg gtatcatcat aaaaatctag cacttcatca caccggcgct ttagttcatc
    11521 tactgattcg gcacttactc gaaccacata gctcagtttg tacatggatt ctttggactg
    11581 atccaaggta gtttccaatt catctacgga atccaatgca tctaagacat tggaatttgt
    11641 ttcattgtca gattgatagg catggttgtc caagtctttt aattcctttt tcttattgcg
    11701 aacggtagca agtgcctttt tgttcgtcac aatctccaca ttcatactag tgtcaattgg
    11761 gaatgtaaat tgttgttgct ggtaatagaa aagttctgag gagggaaact ccatttcccc
    11821 cacaattgta ttaattgtta agtacgctac atatgattca tgattctcat gttccatgca
    11881 taagtaccgg ggcttttctt caatcaaaca acgactggga cgtaataagt cgtaacgttt
    11941 caccaaagtt tccgacttca gctttttctt tggtagctga aattcatatt cttcaaatgg
    12001 tgtacccttt tcgccataga tatgttcaat caaatacatc agatcactcg gtgtgactcg
    12061 tctgacttta aatcttcgag ccaatttact ttccattagt ttttcaagct tggtatatcg
    12121 gcgaatttct tcattggata aggaaacaaa atcgcccatc aaatgatgat tcactccata
    12181 gacaaattct tgaaagccgg agaagaaaga tttcttgaga ttctttagat tgacctcttc
    12241 atctgtcgca atcagtttga agccaataaa aaagcgataa tcaatctgac tatcgccaat
    12301 catggatact aatgcctctg tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg
    12361 cccggtaatt tccttttttg atcgttcttg cgtggcacga atactacttt ctgtggcaat
    12421 ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg
    12481 gacttgatat ttctgttcag gtgacaaaaa ggagtaattg taaggtatta actcatagta
    12541 ggcaaaacat tctccctctt gattaaagac aagattattt tcaatatact taatcgggta
    12601 catagttcac actccttacc agagtaatgg gttcgtccca tcgtttcttc tgttctttga
    12661 ctttctttcc tgcgaaagtg accttaaatc gtaagtgata gctaataaaa gatcgtaaaa
    12721 atcccaatgg tttcttaccg tcaaacgttt tttgcgacat gaaccaagta actgccaccg
    12781 gtatacctaa gtattttaaa aaggccccct ctatcataga tagtggaggc acatgggcaa
    12841 atacgatgac aaacagcaag gataaaacaa accaagccat ctggttaaaa gtcacaggaa
    12901 aaggcagttg aaagtcatta attgcataaa tgaccttttc aactgcccaa atactggtgt
    12961 agctttttat tttcttcatc aaatcattcc tttaaaaaga aggatagttt agaaatcaac
    13021 tacccttcaa taattttgtt aatatggata ctcaaaaaca cctcgactcg ttaccaaata
    13081 attgcctcca atctccaagt ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg
    13141 ttctggtact tctccaaaat caccagcttc tacaatcaag aaatatgcta catcactcat
    13201 atcatcgcaa tctggataat agataatatc atccacatgc tctaccatct cctcaaaaga
    13261 attgaagaaa gcatgttgaa tctcagaagc aacttcacca actggcgttc cttcaagttc
    13321 ctccgctaag ccacacaacc gattaatttc ctcaattggc gtatactcat caatctcaaa
    13381 gggaagttca taatcatgaa ttgcatattc ctcgtattcg tcatttaacc caattcgttc
    13441 tttcacttca tcaaaatcga caggtggggt aaaccacgcc cccaccaatt caccttcgtt
    13501 atatttcccg aggttcgcaa tataaacacg catctgttcc atcgtgcttc ccccttacgc
    13561 accgacaatt cggttaaaca aactgagtaa gacatccttc accccagaag cattaaagac
    13621 caatccgacc gcaatcaaag caatcaccaa aaagccaatc agtttggaaa actcccgctt
    13681 aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag actaaggatt gggcattggt
    13741 taaaaaccat tgatatatgg ataggtaagt tcctgatatt ataaatatct caaacttttc
    13801 cccccattca cacggagcgt gcgactttca ccgcactccg cgttccatcg tgtttatatt
    13861 gaaaagtatt tatgtagtag acctatatca agtttaattc ttgattgcca gctaatactc
    13921 tatatttatt tagtcttatg agctggactt tatttagctt caatagagct atatattttt
    13981 gtattgtttc ttctgtttta gcgtgtatta gacggtgtac cgcctctttt accaaaataa
    14041 gatttttata atcgtcattt ccaccagctc cagttggtac aatatgatga caatgtattt
    14101 cattagcttg ttgaaattct tccagagtta tagcacattt tccatgttga gcagaaaata
    14161 gcgaaattcg attgtctgcg tattctattg tgcgattttc caaagataat ttcattaact
    14221 ggtacatcag taatttatca acacttagat tggtgtggat aaattttctt ccctctagag
    14281 tgtagcaatt cgctaaatac acctttgcca ttggttttct gtgttggata tatccaacag
    14341 ggaaaatagg ttcgccctcg ccagcaacat accgaagcat tttagattta ccatatcttg
    14401 ttgcctcctt tgtcgtaagt tgtctgcctg tttttctcaa tctgcccctt ttgtttgtac
    14461 ctaatctgtt agtgaagata gccattacag ctctattcag tttcatacaa tccagattta
    14521 cattcgttgc aatctgataa taattttgca tacctaatac cattgaatta taaagtcttg
    14581 tttcttctaa ttccgttttc ccttttgagg gagaagcaat tttctttgcc tgtgatacca
    14641 gcttgtcttt ttggtttttg aattgtttat cacttatatg tgatttaaca actaacttct
    14701 ttcttctcgg tatcatttta atcttgaaac caagaaagtc ggagtatctt tttctaacat
    14761 ttacaattct tgttttttct tgtgatactt ccaattttaa tcgttgttca atccattgag
    14821 tgattgctat tttggctttt tcagcacttt ccttgtagcg gcagaaaact ctaaaatcat
    14881 ctgcataacg aaccatatac atttccttta actttgtttt cttcatttct ttgtaagcgt
    14941 tgcttttaca tgggctaccg cttttattga tatgaaccac atagtttttt gtaacaggat
    15001 tttcctgcca ttggctttct acccaatggt ctaactcatt aagaacaatg tttgctagta
    15061 gtggggatat tattccccct tgcggagttc ctttctctgg atatgttatt gtaccgttag
    15121 tcattttgat tggagctttt aatatccttt ttaaaatgaa tatcaagtgc ttgtcccgta
    15181 ttcccatagc ccatatctgt tttatcagct tggaatggtt cacgttgtca aagaaaccct
    15241 taatatcaaa ttcaatcaca taatgtagct ggctgatttg taggcggtta taggttgctt
    15301 ttattgcatt ttcaactgac ctattaggtc taaaaccata gctgttttca ctaaatttag
    15361 cttcgcagat tggttctaat acttgtttta tacattgttg tataagcctg tcccacatac
    15421 aaggaatacc tagaggtctg gttttaccat ttggttttgg tatttctttt cgcctcactg
    15481 gcttaggtcg atacccatgt ggactgcctg caacaatgta tcttactttt ttgacaactg
    15541 tttctgctgg caatttgcca atatctttaa ttattgtacc gtctgttcct gctgttttac
    15601 tgcctgcatt tgctttgata tttcgatatg ccagtaatat attttctctc gataggataa
    15661 tatccataag atgagtgaaa atatctccat tcttactttt ggcatataaa tcatcaaagg
    15721 tgttttgcat ttcgtaatat tcagcgtgtc ttaaatcttc tacacacaat gtttcatttt
    15781 tatttttagg cataaggctt cactccttta cttgaaattg agtgagcttt tttatcttac
    15841 tcgtaatcct tattgaaaca acataatact tttctaatat atacacgact tgagggcatt
    15901 cctccattct cattacaaga atttcttagg ttcgccctct gcttttcagc attagttcaa
    15961 ctgcttattg ttcttcgtca gtttattctc ttgatgagaa cccaatacct ttcctcgttc
    16021 cgataatctt atctatacat atatatcctt aggtcactag ctataagcct gctggcttga
    16081 taccgcctgt aacgttatat ggcatttcat aacctatatt cttactttgc cacaccagct
    16141 acgatttctc gtacctttgc atttctacaa agttcaaatt tagacccgta cattcgctgt
    16201 ttcgtcagtt ccttatagaa cattctaacc atagatattt tatagacccc cgacctgttg
    16261 gatacgctac ccacctctgg atagttttcg atagaagata tatgttcatc aactattacg
    16321 gtatctttca gccgtcttta ccgagcttca aacattctga gtttcctcat acaatgcttg
    16381 tcggaggctc agggcaggcg tttcagagcg ttacttcatc attccacctg tcagattatc
    16441 agttctccta ataaccaaac tttttcgttc ttttattagt gccttagctt tt
//
LOCUS       DAZUKZ010000050        16381 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000050 DAZUKZ010000000
VERSION     DAZUKZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.050"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..551
                     /gene="hslV"
                     /locus_tag="ACG8EF_001614"
     CDS             3..551
                     /gene="hslV"
                     /locus_tag="ACG8EF_001614"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFS4946924.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            565..1965
                     /gene="hslU"
                     /locus_tag="ACG8EF_001615"
     CDS             565..1965
                     /gene="hslU"
                     /locus_tag="ACG8EF_001615"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFS4946925.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1983..2774
                     /gene="codY"
                     /locus_tag="ACG8EF_001616"
     CDS             1983..2774
                     /gene="codY"
                     /locus_tag="ACG8EF_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFS4946926.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2856..3731
                     /locus_tag="ACG8EF_001617"
     CDS             2856..3731
                     /locus_tag="ACG8EF_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS4946927.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="ACG8EF_001618"
     CDS             complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="ACG8EF_001618"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFS4946928.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4628..5062
                     /locus_tag="ACG8EF_001619"
     CDS             4628..5062
                     /locus_tag="ACG8EF_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFS4946929.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5228..7273
                     /gene="parE"
                     /locus_tag="ACG8EF_001620"
     CDS             5228..7273
                     /gene="parE"
                     /locus_tag="ACG8EF_001620"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFS4946930.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7285..9735
                     /gene="parC"
                     /locus_tag="ACG8EF_001621"
     CDS             7285..9735
                     /gene="parC"
                     /locus_tag="ACG8EF_001621"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFS4946931.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10012..12243
                     /gene="pflB"
                     /locus_tag="ACG8EF_001622"
     CDS             10012..12243
                     /gene="pflB"
                     /locus_tag="ACG8EF_001622"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS4946932.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12487..13248
                     /gene="pflA"
                     /locus_tag="ACG8EF_001623"
     CDS             12487..13248
                     /gene="pflA"
                     /locus_tag="ACG8EF_001623"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFS4946933.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13333..14259
                     /locus_tag="ACG8EF_001624"
     CDS             13333..14259
                     /locus_tag="ACG8EF_001624"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFS4946934.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14441..15334
                     /locus_tag="ACG8EF_001625"
     CDS             14441..15334
                     /locus_tag="ACG8EF_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFS4946935.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15468..15593
                     /locus_tag="ACG8EF_001626"
     CDS             15468..15593
                     /locus_tag="ACG8EF_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFS4946936.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15727..16359)
                     /locus_tag="ACG8EF_001627"
     CDS             complement(15727..16359)
                     /locus_tag="ACG8EF_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946937.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         5818 a         2791 c         3493 g         4279 t
ORIGIN      
        1 acatggtcga atcaccattt cattcaacca ctatctgtgc cgtcgaaaaa gacgggaaat
       61 ttgctatggc tggagatggc caagtaacaa tgggtgaatc tgttgtcatg aaaggcactg
      121 caaagaaagt acgacgtatc tacaatggag aagttgttgt aggtttcgcc ggaagtgttg
      181 cagatgcgtt tactttagaa gaaaaatttg aaggcaaact aaacgaatat aacgggaatc
      241 tacaacgcgc agctgtcgaa ttagctcaag aatggcggac acagcaatct atgcaaaaac
      301 ttgaagcgat gctgatcgta atgaatgata aagaaatgct tcttgtttca ggtacaggag
      361 aagttatcac accagatgat ggtatcttag ctatcggatc aggagggaat tttgcgttgg
      421 ctgcagcccg agcaatgaaa aactatgctc aaaacgacat gagtgcgaaa gaaattgcag
      481 aaaatgcgtt aaatattgcc gcagatatct gtatctttac taatcataat attatcgtag
      541 aagaactgta agaggtgagt agatatgtat gaattaaaac aaacaccaag acaaatcgta
      601 agtgaattag atgaatatat cgtaggacag cacgcagcaa aaaaatctgt tgccgttgct
      661 ttgcgtaacc gctatcgccg tttgcagtta gatgaaaaaa tgcaacaaga tattacacca
      721 aaaaatatgc tgatgatcgg acctacaggg gtcggtaaaa cagaaatcgc acgacgtttg
      781 gcaaaaatcg tcaatgcacc atttgtcaaa gtcgaagcaa caaaatttac tgaagtcggt
      841 tatgtcggaa gagatgtaga atcaatggtt cgggatttag tagaaaatgc gatccaaatc
      901 gtagaaaaag agcagtacag ccgtgtatac acaagtgcta aaaaaaatgc agaaaaacgt
      961 ttggtaaaaa tcttggtacc tggtatcaaa aaagaacaga aacaatccac taatcaattt
     1021 gaacaaatga tgaatctttt taatgctagt cagcaaaata ccgctgaacc gcaagaagaa
     1081 gtgacagaag atattcgaat caatcgccgt acagtcttgg aacaactaca aaagggttta
     1141 ctggatagtc gtgaagtaac cattgaagtt gaagaccaaa agaaacctgc accaatgatg
     1201 aacaatggat tggagcaaat ggggatcgac ctaaatgaaa ctctaggtgc tttgacaccg
     1261 aagaaaaaag tggaacggac ggtaacagtc aaagaagctt tagagttatt gatcaaggaa
     1321 gaatcagcaa aattagtcaa ggatgctgat atccatagtg aggcaatcaa attagcggaa
     1381 gaaagtggaa ttatttttat cgatgaaatc gataagatca cttctaaatc acagcaatct
     1441 ggagaagtat ccagagaagg cgtccaaaga gacatattgc caattgtcga aggttcacaa
     1501 gtaaacacaa aatacgggat catccaaaca gatcacgtcc tattcattgc ttcaggagct
     1561 ttccatttgt ctaaaccaag tgatctaatt ccagaacttc aaggacgctt cccgatacgg
     1621 gtagaattag atgatttgac agctgaagat tttgtgaaga ttctgaccga accaaataat
     1681 gctttagtaa aacaatacgt cgcgttgatc ggtacagaaa atgtgtcagt tacgtttaca
     1741 aaagaagcaa ttgaacggat cgctcaaatc gctttcgatg tcaaccgtga cacagataat
     1801 atcggcgcac gcagactgca taccattttg gaaaaactac tagaagatct attatttgaa
     1861 gcaccagata tgcaaatggg agaaataacg atcacagaag catatgtgga tgacaaactt
     1921 ggctcaattg cggcaaatga agatttaagt cgctatattt tataaaaaat cggaggacga
     1981 caatgacaga cttgctaacg aaaacccgca agataaacac acttttgcag caaaagaata
     2041 cttttgacat gcaagcagat ttgccttacg ataaaatggc tgtcacacta ggtggtattc
     2101 ttgacagcaa cacctatatc ataggaagtg acggagtatt actaggttat aatgaacgtc
     2161 acgatgtgaa caatgaacga atcaaaacaa tgtttgttga gaaacaattt cctgaatctt
     2221 atactgatac agtggcacag ttgatgaaaa ctgaagctaa tatcccaatt actagtgata
     2281 ttactgcatt tccggtagag tcaagagaga aatatccatt tggtctgacg actgtcgttc
     2341 cgatctttgg agctggcgaa cgtttgggta cgattatctt atctcgtatg gaacaatcgt
     2401 ttgcagatga cgatctagtc ttagcagaat atggagctac ggttgtcggt atgcagatcc
     2461 tttatcagaa gtctcgaagc atcgaagcag atgtacggag tgcaacggct gtccaaatgg
     2521 caatcaacac attatcttat agcgaattga aggctgtaca agctatcttt gaagcattgg
     2581 atggagatga aggtcgtctg actgcttcta atattgccga tagtatcggc atcactcgtt
     2641 ctgtgatcgt caatgcttta cgaaaattgg aatcagctgg aatcattgaa tctcgttcgt
     2701 taggcatgaa gggaacttat ttaaaagtat tgaattcccg ttttaaagac gagttagcaa
     2761 aacacagtta ttagaagtaa ggggactggg gcagatagaa atgtttgttc cagccttccc
     2821 attttaaaaa gtaagtcgaa gttggaggaa tggcaatgac tgttattatt caaaatgacc
     2881 agctgatcgc cgaaatcgct gaacacggtg cggaattgat cagtctgaaa tcaaaagaac
     2941 atggtttaga gtatatctgg caaggtgatc cagcgtattg gggcagacat gcaccggttt
     3001 tattcccttt tgttggacgt ttaaaagatg atcagtatgt ttatcaaggg aaaacatatc
     3061 aaatggggca acacggattt gctcgggata tggattttga agtcattgaa catggacaag
     3121 aaatggctag tttcatctta aggagtacgc cggagacatt agaaaagtat ccatttgatt
     3181 ttgaattagt gatcagctat gaattaggag gagacggcat cacggttcat tatcaagtgg
     3241 caaataccgg tgaaaaagaa atgttctttt ctatcggtgg acatcctgct ttcaatgtac
     3301 ctcttgaaga aggattgtca tttgaagatt tctatctggc attttctcca ttgaaatcaa
     3361 gattacgcct accattgaaa ggcccattca tcgatatgga tcaaaagacg ttaggtcaga
     3421 caaatacgaa tctggctttg aatcgtgaac tttttgagca agatgccttg gtttttgaaa
     3481 caaggggtct aaatgcgtac gccattcgaa gtgaaaaatc ttcccatagc gtaactttaa
     3541 gctataaaga tatgccttat gtagggatct ggtctcctta tccaaaagaa gcgcctttcg
     3601 tttgtatcga accgtggtgc ggaatcgctg acacggtgaa cagtagtgga aacttgatgg
     3661 aaaaagtagg gatcaatcaa ctaggtgccc atgaaatctt caagacaaaa tattccatca
     3721 cagtgaagta agaggttgtg accgagaagt tagcagggaa caccgtttac caaggtgtgt
     3781 aaaaaagcta tcctgtaagt aataagggag tgtgacatcg atttgtcaca ctcccgtttt
     3841 atctctttgg aatgaaaaag tatcaggatt ttttcttgct gctattcaaa ccaaacggga
     3901 ttcgactttc agtatggttt ctgatacgct tgatattgtc tttatgccga taaaagataa
     3961 aggaagaaac taaaaaggca atgatcgtca gtaaccaatt gggcttagcc aaaatcacag
     4021 gagctacata aggaaggacg atcgtagata aagtgatcaa tactgcactg atcatactgc
     4081 tcaaactcac catacttgta caaaataaac taacgataaa gatgatacaa gaataaacaa
     4141 aaaatacagg attaaatcct aaaagcattc ctgcacttgt cgcaacagct tttcctcctt
     4201 tgaacccagc aaaaatcgga aatgtatggc caagaatggc gcatacccca aaccaaagag
     4261 ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt agccaatgta ccttttaaaa
     4321 tatccataag caggacaaca atcccggctg ttttgcctaa tacacgaaat gtattggtcg
     4381 ttccagtatt accgctcccg tgttgtcgga tatctttttt gaaaaacaat ttgccaatcc
     4441 agacaccgga aggaatagaa cctaaaagat atgctaaaat aagtaataag atcaatttca
     4501 ttttttaccc tctttcaagt tgaaaactat cctttatttt agcatgaata ggtaacagga
     4561 acaattgaaa aaatgaaaga tacaagtata atagatggaa taaaaagaaa agcaaggggt
     4621 ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt ttcaactatt tggaagaagc
     4681 aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa cgaacaagtt acatggttgc
     4741 acaggtattg caagacaatg gatataaaat cataccagta aatccattat tggctggtga
     4801 agaaattcta ggcgagaaag tctatgcgaa tttgacagaa atcgatgatc caatcgatat
     4861 cgtagatgta tttagacgga gtgaattttt acctgaaata gcaggtgatt ttttgaaaac
     4921 tgatgctaaa gtgttctggg cacaattagg gattgaaaac aaagaagcag ctgagatgct
     4981 aagaaaagca ggacgtacag atatcgtaat ggatcgttgt attaagattg aacttggtaa
     5041 gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga gatgatcgat agaggggaat
     5101 gtcgaaaaag cggttaggag ataattaaaa ttgatttctc gtctaccctc ttttttttca
     5161 cgaccttttc tagtatgata atcgtggtgc ggttttgccg caaagtatta ttgaaggagc
     5221 gtttgccttg gcaaaaaaag taaacaacga atacaatgac tcgtccatcc aagtattgga
     5281 aggattagag gcagttagaa aaagaccagg gatgtacatt ggatccactg acagccgagg
     5341 cttgcatcat ttagtctatg aaatcgtcga taatgcagtt gacgaagcat tatcaggtta
     5401 tggcagtgag attgatgtga ccatccatga agacaatagt ataactgttg cagatagcgg
     5461 acgtgggatg cctgtaggaa tgcatgcttc cggaatacca acagttgaag tgatttttac
     5521 tgtgctgcat gctggcggaa agtttggtca aggagggtac aaaacttctg gtggactgca
     5581 cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg ctaattgtaa cgatcgttag
     5641 agatggcgta gagtatcagc agaaatttaa aaacggcggt aaacctgacg gtactttgaa
     5701 aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc cattttttac cagatgatac
     5761 aatattcagt acaacgaaat tttcatatga aattttagcc gaacggttaa gagaatctgc
     5821 ctttcttcta aaaggcgtta aaatctctct ttctgatctt cgtggagaag aaccagtcaa
     5881 ggaaattttt cattacgaag aaggaatcaa agaatttgtt gattatctga atgaagaaaa
     5941 agatacattg acaccagttg tttatttttc aggagaaaaa gaaggtatcg aagtagaggt
     6001 cgcttatcaa tacaatgacg gttattcaga aaatgtttta tcttttgtca ataacgttcg
     6061 gacaaaagat gggggaaccc atgaagctgg tatgaaagcg gcgatgacga aatcttataa
     6121 tgaatatgcg agaaaagttg gtttgctgaa agaacgggac aagaatctgg aaggtagtga
     6181 tttcagagaa gggttagctg ctgtactttc tatccgtgta cctgaaaatc ttctgcaatt
     6241 tgaagggcaa acaaaagaaa aacttggtac gccagttgct cgtaccgtag tagataacgt
     6301 catcagtgaa caaatgggct tttatttgca ggaaaatagc gaaatgagcc agatgttggt
     6361 ccgtaaagca atcaaagcca gagaagcgag agaagcagca cgtaaagcca gagaagaaag
     6421 ccgaaatggg aagaagcgta aaaaaggcga atctcttctt tcaggaaaat tgacacccgc
     6481 acaatcccga aaccctaaga aaaatgaatt gtatttagtc gaaggggatt ctgctggcgg
     6541 atctgctaaa caaggaagag acagaaagtt ccaagcaatt ctacctttac gaggaaaagt
     6601 catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa aatgaagaaa tcaatacgat
     6661 gatctatacg attggtgcag gagtcggtcc tgagttttct atcgaagact gtaattacga
     6721 caaagtaatc atcatgaccg atgcggatac agacggcgcc catattcaag tattactgct
     6781 tactttcttt tatcgttata tgaaaccgct gattgaagct gggaaagtat atatcgcgtt
     6841 accgccttta tataaagtat ccaaaggaca aggaaagaag caagtcattg aatatgcgtg
     6901 gacagatgac gaattggcag caatgattaa aaaagtcggc aaaggatata tgctgcagcg
     6961 atataaagga cttggagaga tgaatgcgga acaattgtgg gaaacaacga tggatccgac
     7021 ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg caagctgaac gtcgagtaac
     7081 cacactgatg ggtgacaaag tagaacctcg aagaaaatgg atcgaaaatc acgtgcaatt
     7141 tacgctcgaa gaagatggca gtatcttaga taaaaaagag gatactgaaa tctctccttc
     7201 agtttctaat gatttattag atgaagaacg agcagataaa aatgaaaaca atcagttatt
     7261 tgaagtagaa taggaggtca cctgatggaa aatcgacaag aagtacaaga attaacttta
     7321 gaagaagtga tgggtgaccg cttcggacgt tattccaaat atattatcca agaacgtgca
     7381 ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc ggatattatt tgccatgaac
     7441 aaagatggca atacgtatga caaaggattc cgtaaatcag caaaatctgt cggaaatatc
     7501 atggggaatt atcatcctca tggtgacatc agtatttatg aagcgatggt ccgcatgagc
     7561 caagactgga agttgcggga agtactgatc gaaatgcacg ggaataacgg gagtatggac
     7621 ggtgatccac ctgctgcgat gcgttatact gaagccagat tatccaagtt aagtggagag
     7681 atgctagcag atatcgagaa agaaacagtc gatctcgtgt ggaattttga tgatacagaa
     7741 aaagaaccaa cagtccttcc agctcgatac ccaaaccttc tcgtcaatgg atcgacaggt
     7801 atatctgctg gatatgcaac agaaatcccg actcataact tagctgaagt gatcgatggc
     7861 acaatctata tgatcgatca tccacaagcg agccttgaaa aattgatgga atatattcct
     7921 ggacctgact ttccaacagg agggatcttg caaggaaaag atgaaatcaa aaaagcttat
     7981 gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa agatagaggc aatgaagggt
     8041 aataaacagc aaatcgtgat cacagaaatc ccatatgaag taaataaagc gaccttggtg
     8101 aaaaaaatgg atgagatccg tttgaataag aaaatcgatg gaatagctga agtacgagat
     8161 gaaagtgata gaacaggtct gcaaattgtc gtcgaattga aaaaagacgc caatgctcaa
     8221 ggaattttga attatttgtt caaaaacact gagctacaga tcaattacaa tttcaatatg
     8281 gtcgctatcg accatatgac tccgcatcaa gttggactga aagacatttt aagaagttat
     8341 atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat tcgatttagc caaagctcaa
     8401 aaaagacagc atatcgtaga aggattgatg aaagctttat cgattctgga tgaagtaatc
     8461 gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa agaatttagt cgacgtcttt
     8521 caatttacag aagaacaagc agaagcaatt gtcacattgc aattataccg tttgacgaac
     8581 acggatatca cagagcttca aaaggaatcc gaaagcttga ttgcgcaaat cacagagttg
     8641 aataaaatcc tttcaaatga caaagagctt ttctctgtta tgaaaaaaga attgcgagaa
     8701 gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg aagacgaaat cgaagaaatc
     8761 aaaattgata cgcaagtctt agtggcacaa gaagacgtaa ttgtcagtgt gacgcgagaa
     8821 ggctatgtga agcgaacaag ccttcgttct tatagtgctt ccaaaccaga agaaatcggg
     8881 atgcgagaag gcgactactt gctatactca ggtgaattga gtacattgga ccatgttctt
     8941 ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc atgaactacc cgatttgaaa
     9001 tggaaagatg ctggtgagca catttctcaa acaatctcca atctgtctgt agacgaatcg
     9061 attttagctg ttttcccata tcaaaaaatt caagcagaga aaacatttgt attcatcagc
     9121 aagaatggat tgatcaagca aacacgaatg acagagttcg aaccatggcg cacctataag
     9181 agccgtccgc tcagctgtat gaagctaaag gcggaagaag atgaactgat tgctgtgtat
     9241 ctgaaagaag atcagccagg cttagatgtt ttcttagtaa cgcatcaagg aatgggtttg
     9301 cgctatcctt taacagatgt tcccgttgtt ggagcaaagg cagctggagt gaagtctatg
     9361 aatttgaaag atggagatag gattatcaac gggctgcttg tgttagcaga aggtgatacg
     9421 ccgatcgtaa tcgttactca gcgaggagct gttaaacgca tgcttgctca agaaatcagc
     9481 caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg agctgaaaaa aaatccgcac
     9541 cgtattatct atatgtcgga aggaagatca aaagaaataa ccttgatgaa tcaaaaaggg
     9601 cagcagttaa cgattgaccc aactgatttc cctataggtg atcgaacatc gaatggatca
     9661 tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag ttatcgatgc tcctgagatt
     9721 ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga cgccatctgt attaatacag
     9781 gtggcgtctc tttttcattc atttttaata aatccttata cgtttgcaaa aaaagatgaa
     9841 agtgttcaca atgcttaaat gttggtatgt caacgttgat ttaaatatat gtaaataaaa
     9901 cataaaaatt gttaaataaa aaacaagaaa acccttgcaa agatagggtg ctgtgttata
     9961 ctaatcacgt aaaaactgtt acataaaaca gaaatttagg aggtccatac aatggagcaa
    10021 tggaatggat ttaaaggtac caaatggaaa gaaggcgtag acactagaga ctttattcaa
    10081 aataactaca ctgaatatcg cggtgatgac agctttttgg aaggcccaac agaagcaaca
    10141 gaaaaattat gggatgaatt gcaaaagtta aatgatatcc aattcgagcg taacggtgtc
    10201 tatgatatgg acacagaaat cgtatcaact attacttcac acgatgcagg ctatttggat
    10261 aaagatttgg aaaaaatcgt cggtctacag acagacaaac ctttgaaaca agcattcatg
    10321 ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac
    10381 gatgaattga cacatatttt cactgactgg cgcaaaacac ataatcaagg tgtatttgac
    10441 gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa tcattacagg gcttccagac
    10501 gcttacggtc gcggacgtat catcggtgac tatcgtcgtg tggcattgta tggaatcgac
    10561 tatttgatgg aacaaaagaa aaaagatcat gatatgactg gttatcatac aatgagtgaa
    10621 gaagtgattc gtttacgtga agaaatcact gaacaatatc gtgcattagc gcaaatgaaa
    10681 gaaatggctg cgaaatatgg ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct
    10741 gtccaatggc tatacttcgg atatttagca gcaatcaaat cacaaaatgg tgctgcgatg
    10801 tcaatcggac gtatctcagc attccttgat atctatatcc aacgtgattt ggataatgga
    10861 gtaatcacag aaaaagaagc acaagaattg atcgaccact tagtaatgaa attacgtatg
    10921 gtcaaattcg cgcgtacacc tgaatataac caattattct caggattccc tatctgggct
    10981 actttatcca ttgcaggtat gggattagat ggtcgttcat tagttacaaa gaatgatttc
    11041 cgtctgcttc atacattaac aaatatggga ccttcaccag aaccaaactt gactgttttg
    11101 tattctgaac accttccaga aggattcaga acatatgcta gcagaatcgc tatcgaaagt
    11161 tcttcgattc aattcgaaaa tgataatcag cttcgtgaac aatggggttc tgatgattgt
    11221 gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag acatgcaatt ctttggcgct
    11281 cgtgctaact tggctaaaac actattatat gccatcaatg gcggagtcga tgaagtgact
    11341 ggcgcccaag ttggaccaga atatcgtcca atcacatcag aaatactaga ctttgaagaa
    11401 gtgaaacata acttcatgaa tatgatggat tggttagcag aactatatgt caatacgttg
    11461 aacatcattc attatatgca tgataaatat tcttatgaag cagctcagct ggctttgatg
    11521 gattctgact tgcaacgcac atttgctaca ggtattgctg ggatctctca tgcggcagac
    11581 agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca
    11641 gttgattttg aagtggatgg cgacttccct aaatacggaa atgatgatga ccgtgcagat
    11701 gaattagttt catggatctt ggaatacttc atgggacaaa tcaaacgtca taaaacttac
    11761 cgcaactcaa aaccaacaac ttcattattg acgattactt caaatgttgt ttacggtaaa
    11821 aatacaggaa atactcctga tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat
    11881 ccaagttaca atgcggaaca aagtgggtta ttagctagcc tgaactcaac agctaaaatg
    11941 aattacggct gtgcattgga tggaatttca aatacccaaa caatcaatcc ttcaggtctt
    12001 ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg tcttagatgg atacttctca
    12061 aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg
    12121 gaaaatccaa tgaaatatcc gaacttgacg atccgggtat ctggttatgc agttaaattc
    12181 cgtgacttga caagagaaca acaattagat gtcttgatga gaacttcaca cgacagaatg
    12241 taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa aggacgttga atagccatcg
    12301 catcaaaatc gaataatcag tatgcttatt tgaaagaaac gatttacagg ggcaggactt
    12361 gtatgttccg tccctgtaaa ttttacacat aacgaagcgg caattgcgaa aaattttgtt
    12421 cagaaagctt cgcttcgaca gctaacttgt acttaaaatg aaatcaaaga aaagatgtga
    12481 acttaaatgg aagaaaaaac aatcggctat gttcattcta tcgaaacttt cggttcagta
    12541 gacggacctg gactgcgctt tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt
    12601 tgtcacaatc cagatacttg gaatattggc ggagggaaag aatacacagc agatgaactt
    12661 ttagataaag cagaacgctt cagaccttac tggggagata aaggtggtat tactgttagt
    12721 ggtggagaac ctctattgca gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag
    12781 cggaagatgc atactacatt ggatacatgc ggaaaacctt ttacgtatga agaacctttc
    12841 ttttcacgtt ttcaagaatt gatgaagtat acagatttac tgttatttga catcaaacac
    12901 atcgataatg aagagcataa aaaactaacg cattgggaca atgaaaatat tttagaaatg
    12961 gctcaatatt tgtcaaaaat caacaaaccg gtttggattc gccatgtact agttcctgag
    13021 cgaagtgatt atgatgaata tttgatccga ctagacaact ttattcaaac cttgtcaaat
    13081 gtagacagag tagagattct tccttatcat acgatgggaa aatacaagtg ggaaacactt
    13141 ggtttgaaat atccattaga agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct
    13201 aaacgattgc ttcatacaga agagtataca aaatatttaa cgcgataggt cgcatcggaa
    13261 agaagataag tattgcttct ttccgttttt ttgctattat taaataggag aaatcacaaa
    13321 ggagcgaatg acatgtcaaa aattttagtt tttggtcacc aaaatccaga tacagatgca
    13381 atcggggcag ccattgcgtt ttcacacttg caaaatgaat taggtaaaga aacagaagca
    13441 gttgctctag gtacacctaa tgaagaaaca caatacgcat tagaccactt tcagctatct
    13501 gcaccacgag tagtagagtc agctaaagca gaaaatgcag atgtcatgct tgtggatcat
    13561 aatgaattcc aacaaagtat ttcagatatc agcgaagtaa atattttagc tgtagtagac
    13621 caccatcgta ttgcaaactt tgaaacagct aatccattgt actatcgtgc agaaccagtt
    13681 ggatgtacaa gtacgattat tttaaaacta tataaagaaa accaagtaac aattcctaaa
    13741 gaaatcgcag gaatcatgct ctcagctatc atttcagata cattgttgtt caaatcacca
    13801 acatgtacgc ctgaagatga agcagctgca aaagaacttg cagcaatcgc tgaagtcgat
    13861 ctaaacactt atggacttga aatgttgaaa gcaggaacaa atctaagtga taaatcagct
    13921 gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc
    13981 gctcaagtca atacagtgga ccttaatgaa gtgatggatc gacaagcaga gctagaagca
    14041 gcaatggaag atgaaatgaa agcaaatggc tacgatctgt tcgtattagt tgtcacaaac
    14101 atcttagaca gtgattcaga attacttgtt gctggtaaag gaaaagaaaa agtagaagaa
    14161 gctttccaaa caactttgac caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa
    14221 aaacaagtcg tacctcaatt gacggctgta tttagctaat caaatggtag tatgaagcat
    14281 tcaaaatgaa tggataagcg attgatataa aaagaaacat agaacatact aaaggccgaa
    14341 gaaatggagg agaataacag tcaattatga ctcttgttct tcctcctttt tacgtaaaag
    14401 ccttttaagt aaaataaaac ggatagaaaa gagaacatat atgaactatt attcaaaaaa
    14461 taaggaaaac ttttatcaaa aactgaccga tgatccgctt ttttcttcat tgaccgatta
    14521 tctatatgaa catcgagaac aagaaacgat tctaagagaa ttgaaaaaag aattttcgca
    14581 aaataaattt tcccattttc ttgatttatt gatcgatgca ggattaatca agagggaaga
    14641 acgaagatat catttgaact ttccaatatt tgatcctaaa gattacttgc aacaagccac
    14701 ttcggctgct gaaaccatag ctgaacaatt gaagagactc tctgttgatg aacaaaaatt
    14761 agcgatggga gaagttattt gggcgtattg tttcgaagat gaacggaaag aagcgtattt
    14821 ttatggagta agaaacagcc gagagacaga attgctgagg acaactgcag gaaatgagaa
    14881 gtaccgtttt atcacacttt cctctataga acacttcccg cttactttgg ctaattactt
    14941 ttttgtacag aagaatcagc ttcctgtaac gaaggcattc aaagaattag ccgaacttat
    15001 tggagacgta aatgaatcgt acttttttga tcaaatagaa gttatcgttg acaggatacg
    15061 taagaataaa tataaaaatc gccggcctag tattttccat cagtctctac ttgtgacgaa
    15121 tactataaag gaagaagaat cattcacatt agaattacct attgtcgaaa aaaacaattt
    15181 tgagattgaa ttaccaacat tggatccttc attgacaatg gaggaaatgg cctttcttaa
    15241 acgacaaatc ttttctgagc tgagcaagaa atttataccg catgcatttt cttatatcaa
    15301 agaatatgga acagttttag tgagtaaaac ctaagagagc agtaagcaaa aattatactg
    15361 actcttgcag gaggagcctt tctcttcctt tatggttaaa agaattattt gagtattttg
    15421 cctatctacc acctattaga ttcactacat ggtctggaaa caaaggaatg caaaaaaatg
    15481 gtgagaaatg tggaatgaca aaggaagtag tgataagaaa agttagattt ttaaataatc
    15541 aatattatga ttcagtcaag tatggcattt tatgggaaga actagctgat tagattagga
    15601 agtccatttg ataatccaga atcactaaga aacgaatggc gagtcattaa taatttatcc
    15661 aatcttgggt tattctttta gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg
    15721 taatagtcag gcttcaaaat gctttttatt ttgtttgttt tttctttgcc acataaatac
    15781 acctagactg acgatgaaga gcatatccaa cagatttcct gcgacttgga tcgtcgaagg
    15841 gttggtcagt ggcattaaag caaggacaaa aaaggaaaac aagctgtaaa taaaacaggt
    15901 gacaggaact aatcgaaaga agagcggaca tttcaattgg ctaaaaatga gtgaaaaaat
    15961 aaaataagta aaaatggata aaatcacgac taaaccattc atgataaaga agaaaagcgt
    16021 attgtgatta aacgaataaa aaagaattgc taaaaaagaa aacgttttaa atgcaatcaa
    16081 tacgcttgaa gcttcgttta acgtaccagg atgtcgatga aaaaaatagg caccgaaata
    16141 aacagaaaac agtaagccgc aaagagaaga caaaatcaaa aaaaacggat tttcttcggc
    16201 ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat gcactagaag gcgcatgtga
    16261 ggtcttaagg tatgaataac atagaaaaaa taagatcgtc aaagttgcaa aaaaagagcc
    16321 taaatgtaaa aaattagtgg ttctacgttg aatttccata atatctccaa attaaattga
    16381 t
//
LOCUS       DAZUKZ010000051        16310 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000051 DAZUKZ010000000
VERSION     DAZUKZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.051"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..622)
                     /locus_tag="ACG8EF_001628"
     CDS             complement(<1..622)
                     /locus_tag="ACG8EF_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946938.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPE"
     gene            complement(801..1520)
                     /locus_tag="ACG8EF_001629"
     CDS             complement(801..1520)
                     /locus_tag="ACG8EF_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4946939.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1651..2802)
                     /locus_tag="ACG8EF_001630"
     CDS             complement(1651..2802)
                     /locus_tag="ACG8EF_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFS4946940.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3019..3759)
                     /locus_tag="ACG8EF_001631"
     CDS             complement(3019..3759)
                     /locus_tag="ACG8EF_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4946941.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3763..4086)
                     /gene="rsfS"
                     /locus_tag="ACG8EF_001632"
     CDS             complement(3763..4086)
                     /gene="rsfS"
                     /locus_tag="ACG8EF_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFS4946942.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4143..4736)
                     /gene="yqeK"
                     /locus_tag="ACG8EF_001633"
     CDS             complement(4143..4736)
                     /gene="yqeK"
                     /locus_tag="ACG8EF_001633"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFS4946943.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4720..5370)
                     /locus_tag="ACG8EF_001634"
     CDS             complement(4720..5370)
                     /locus_tag="ACG8EF_001634"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFS4946944.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5380..5691)
                     /gene="yhbY"
                     /locus_tag="ACG8EF_001635"
     CDS             complement(5380..5691)
                     /gene="yhbY"
                     /locus_tag="ACG8EF_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFS4946945.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5707..6816)
                     /gene="yqeH"
                     /locus_tag="ACG8EF_001636"
     CDS             complement(5707..6816)
                     /gene="yqeH"
                     /locus_tag="ACG8EF_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFS4946946.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6813..7340)
                     /locus_tag="ACG8EF_001637"
     CDS             complement(6813..7340)
                     /locus_tag="ACG8EF_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFS4946947.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7520..8305)
                     /locus_tag="ACG8EF_001638"
     CDS             complement(7520..8305)
                     /locus_tag="ACG8EF_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFS4946948.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8298..9167)
                     /gene="accD"
                     /locus_tag="ACG8EF_001639"
     CDS             complement(8298..9167)
                     /gene="accD"
                     /locus_tag="ACG8EF_001639"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFS4946949.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9168..10547)
                     /locus_tag="ACG8EF_001640"
     CDS             complement(9168..10547)
                     /locus_tag="ACG8EF_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFS4946950.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10555..10974)
                     /gene="fabZ"
                     /locus_tag="ACG8EF_001641"
     CDS             complement(10555..10974)
                     /gene="fabZ"
                     /locus_tag="ACG8EF_001641"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS4946951.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10999..11475)
                     /gene="accB"
                     /locus_tag="ACG8EF_001642"
     CDS             complement(10999..11475)
                     /gene="accB"
                     /locus_tag="ACG8EF_001642"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFS4946952.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11478..12713)
                     /gene="fabF"
                     /locus_tag="ACG8EF_001643"
     CDS             complement(11478..12713)
                     /gene="fabF"
                     /locus_tag="ACG8EF_001643"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFS4946953.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12732..13469)
                     /gene="fabG"
                     /locus_tag="ACG8EF_001644"
     CDS             complement(12732..13469)
                     /gene="fabG"
                     /locus_tag="ACG8EF_001644"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFS4946954.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13481..14398)
                     /gene="fabD"
                     /locus_tag="ACG8EF_001645"
     CDS             complement(13481..14398)
                     /gene="fabD"
                     /locus_tag="ACG8EF_001645"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS4946955.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14505..14729)
                     /locus_tag="ACG8EF_001646"
     CDS             complement(14505..14729)
                     /locus_tag="ACG8EF_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS4946956.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14782..15744)
                     /locus_tag="ACG8EF_001647"
     CDS             complement(14782..15744)
                     /locus_tag="ACG8EF_001647"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFS4946957.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15754..16194)
                     /locus_tag="ACG8EF_001648"
     CDS             complement(15754..16194)
                     /locus_tag="ACG8EF_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4946958.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         4296 a         3717 c         2790 g         5507 t
ORIGIN      
        1 tttcaggata ttttaatttg acaagcgaaa taatcatttt cagttcttcc atgttttctg
       61 gatattctgg gtcaaatacc ttgctatgac ggccaatccc gccattgtcg atcaatcggt
      121 aaattcgatt ccattcaatg aattggtcaa ttgattggaa attgctgatt ggatagccgc
      181 cacaatccaa aatctctgat acacgaaatg ctaggtatgc actatcgatc actttatttt
      241 ttttcgtgtc tatcttctca tttctttcga taagaatttt ttcaggacga tgtttcaagc
      301 tccactctaa tagtagaaag aaagcctcca tccgatactc ttcttcaaat aaccgaaagg
      361 ctgaatcaga acgacgaaaa agcagcggaa tcagataatt ttgtctataa atcagcattg
      421 gtgcgcggtc caccatacac tctaaataac ggttcagata ccctgtaccg acatagtttc
      481 ctaatcgata gccttcaacg ccgaatcttg cgtatttttg cgctaatttt gaacaatcag
      541 ttacgttcac agctaactct tctactttgc ggtaaccgct taagatacgt tcaaaaggtt
      601 ctaaagtgat ttttgcttcc atattcttcc ccgtccatct tccacatact tcatatcatt
      661 tcacatgata cttatgctct catcatacca caggttgaat tagaaaagaa aataaatcac
      721 acaaaaaagc aagacaatct gttagtgaga ttgtcttgct tttcgaaaaa gactgaatac
      781 ttttcgttaa aactggttgc ttagtcttct aatgccacat tatggaacac atgctgtaca
      841 tcttctagat cttccagtgc atcaatcatt ttttcaaatg tttcttgatc ttctggagat
      901 agtgttacct cgttttgagg gatcatttgg atctcagcga ttgaaaaatc agtgattcct
      961 ttttctttta atgcttcttg tatactatgg aaatccgcta cttcaccgta aacgatgatt
     1021 tgtccttctt cttccattac atcgcgaaca tcgatgtctt tttccattag gtattcaagg
     1081 atctcgtcag cgtcttctcc tgcaaaagca aagattgctg tattgtcaaa catgtaagaa
     1141 accgctccgg aaacacccat gttcccgcca tttttcccaa atgctgcccg tacgtctgct
     1201 gctgttcgat tcacattgtt cgtcaatgta tcaacaatca ccattgagcc gcttggtcca
     1261 aatccttcat agcgcaattc agagtatgtt tcatctcctg caccttttgc tttttcgatt
     1321 gctcggtcaa taatatgttt tggtacatta taagttttag cgcgatcaat gacgaaacgt
     1381 agtttttggt ttgcatgcgg atcaggatca cctgattttg ctgccgcgta gatttcaatc
     1441 ccaaattttg cataaatccg gctattatta gcatctttag cagttttctt tgctacgata
     1501 tttgcccatt tacgtcccat attttcactt cgctttctga tttgatagtt tcataacgca
     1561 aaaacgtcct gttccattat acttgtgaat cacgattctg tgaagctttt tttctttctt
     1621 ccttcattct tctcattttt agtcaattat ctatactcgg attgggattt gcccgaaatt
     1681 ctgttccgga atattgcctc ccaattgata gatttgatca cttctaacta acagttccgc
     1741 ttcttgtgcc tctttttttc cgattcggga ataaatagaa atctcttttt tttcttttaa
     1801 gaatcttctt ccagcatcgg taaatccaag tagtcgaaga taatttttat tttgttggac
     1861 aggaatctct tgctttttga tattcagcaa gacataacaa gataatcgtt ggatccgtgt
     1921 ccatgtatat ctttttgttt ttacctttga cacaaaatcc tcaaaactat cagcagttgc
     1981 agctgctgtt ttcatgcgcg attctattcc ttcggtcatc tgatagatat ctgtcagttc
     2041 cagcaaagta gaagataaca accgataacg caaatacggc caataagatt cccaagtctg
     2101 cagcgaacaa gttgaaagat cttcttctgt ctgaggcggc acaagtccag ctattctttt
     2161 tccttcttct aacgcatggc gaatggctgt tgcactggct atcctctgat ctgtgatgat
     2221 tttttcatga tagcctgcac ttttacgtcg gatcggatgt aattccatag ggtggacata
     2281 cttggcattt tcctttgcat aacttaatcc caagatatga tttggcgaag aaaaatcaag
     2341 cgtaatatcg ggatacagac tgcgaaaaac ctcagtcatt ttttgcggat aactatacgt
     2401 ttgatcagac agttcttgaa atgcctcttc tatcctatct tgattctctt ggacaaagcg
     2461 tccgaaactg ctgtaatcaa acgaagtctc ttcttctgtt ccaaaacaca aactgtcaca
     2521 gtccagtgtt tgcaagattt ttactgcacc tttagcaaaa tagtcagctg actgaaccgc
     2581 ccattcgacg ggaagctcca ctactaaatc cacaccattt tgtaaagctt cattcgcgcg
     2641 atgccattta tcgatgatag ccggttcgcc tctttgcaaa aaattcccgc tcattacagc
     2701 aatgacgacc tctgctcctg ttatttttcg tgccatttcc acatgatagc gatgtccatt
     2761 gtgaaaagga ttgtactcta cgataatccc gcagcttttc atgcgcatcc tccttcatag
     2821 ttatcgataa aaaagaaata gagacaagta aaaaaaggca aaaatcggaa aaaatacccg
     2881 aactatataa actattctcg caaacacaat acttctgcga cgaaaagctg cactgcaaga
     2941 gcaatgccta tatcgttcgg atatataatt tatgttgaag atttattctt ttcttgttaa
     3001 ctcgagcagg caaaaccgct agtctttgta tgctacaaag aaccagcgct ttgatttctc
     3061 tgatgctgct tcgtctgtaa aatcagaaaa tgcttcgact ttcgaaaagc cgctgttttc
     3121 cagcattctt aaataacttt ccaatggata ggtacgctct tgatgtagtt catcttcacg
     3181 aataaatcga ccagaccctt cttgttcttc aacgaaaaag gtaagaaaat gttcgatgct
     3241 gtgtttctcg cttcctggat aactatccca caaaaaagca aaattatccc cttgataatg
     3301 atagctatat tctggaaaaa cctcatctac ttgataggtg gagtgtacgt caaataaaaa
     3361 tcggccattt tcttctaacg cttgatagac ttcatcaaac acttgctgta cctcttgtct
     3421 atttttcata tagcacagcg aatcagaaaa acaggtaatt gcctgatatt gtccgatttc
     3481 tgataaatcg agcatattgc cttccacaaa ttggatcgat gcgtcttctt catatgctcg
     3541 ctggctggca atcatcaaca tctcttctga aaggtctaac cctgtcactt tatatccatc
     3601 ttttgcaaag gcaaccgcta atgctccagt accgcatgcc agttccaaaa tattttttgt
     3661 gtcttcttgt aaatgacgtt tcgaaaaagc taaccattgc tgataaagtg tttcgtccat
     3721 cacttcatcg taaatgaaag caaatgtttc ataagccata tgttaatcca accattcact
     3781 aagatcgact agcggagcat ctgaccatag tttttccaga ttatagaaat ttcgttctgg
     3841 cgcatggaaa acatgtacga tcacatctcc aagatcgatc aatacccatt ttccgccttc
     3901 ttttccttca atacgtttta cttcatattt gttttcttct tctttatcaa tgatttcttc
     3961 tgtaattgcg ttgatttgac gttcgctatt tgctgaacag atcatgaaat aatctgccaa
     4021 caaggaaaca tttcgaacgt ctaaagcgat aatgtcttct gcccgttttg aatctgctgc
     4081 ttcaatcgca atttttaatt gttctttact atctatgatg atttcctcct aatatgagca
     4141 gcttattttt ttgctaccca gtaattatat gtttcgattg tttttggata aatctgttgt
     4201 tcttgcgcaa gcaaatgggc caatgtatgt tttgtttcaa atgctacagc ttcgtctaga
     4261 tcagcccatg caatggctcg tgcatcttgt accccaggaa aatctcgacc tggttcaata
     4321 tagtctgcta cataaatgat tttgtcaagg agactcatct tagcagcccc agtcgtatgc
     4381 acccggattg catgaagaat ctctgcatcg gtgattccta attcccgctc tacaaacgaa
     4441 gcgccaacaa gaccatgcca gattgcatta ttataattca ataactccgg atcaaaaccg
     4501 tctcttacga ttaccatttt gaactcctca tctggtcgtt cttttgcata atcatgtgtc
     4561 aatgcagcaa tactagcttt ttcaggagaa gcaccgtatt ttttggcaag agccacggct
     4621 gtttcttcta ctccaagcac atgcttgaag cgttgttcgc tcattctcat ctgtattttt
     4681 tgcatcaggt tttctctaga ataagcagta tattcattac tatagtccat attcatacaa
     4741 cccttcattt tgaatataat caattacttt atctggaaca aggtagcgga tagaacagcc
     4801 ttctttgatc ttttgacgta ttttcgtgga actgatgtcg atctcaggta catctaccca
     4861 aattacagga tatggagtat ccgttgtgta tcctggtcga cgtataccca caaagttgac
     4921 catagaagtc agctcatcga ttttatacca ttttggcaaa tattctacca tatccccacc
     4981 gataataaag taataatctg tatccggatt gttttgcgtc aattccttca tagtatcata
     5041 ggtgtaactt ttcccaccac gagctaattc aatcgtttca atctgcaaaa aaggattgtc
     5101 ttccaccgct aattccaaca tgttgagtcg atgttttgca tcaatcgtct gtttctcatc
     5161 aacatgaggt ggctgataag aaggcatcaa aaatacgcgg tcaagaccta aattccggcc
     5221 agcttgttca gccatcacta aatgtgcaag gtgaacggga ttgaatgttc cgcccaagat
     5281 ccctacttgc tttttcttct ctaagaattg aggcatttct tcaggaaata attgtgaacg
     5341 aacaaatgtc ttagcttgcg tattcatcat ggtcactcct tatattgctt ttacttcttt
     5401 tgatagcctt tgatactttt cttttgtgga aggtttgtat aatacaataa cacgtccaat
     5461 gatttggacg atttcgcaat ctatttcttt ttctaatact tgagctgctt cttctgcaat
     5521 ctcatctgta ttttgaagta acgatacttt gatcagttct cttttttcta aagcttcttc
     5581 aatttgaacg atcattgctg aattgatccc accttttccg atttgaaaaa tcggctgaag
     5641 atgatgcgct ttgctacgta aaaaacgctt ttgttttcct cttaaactca taattttcct
     5701 tcttccttaa atcaatgctt tgcgtttcaa tacatctaca ccctttggcg cccagcccgc
     5761 aattactact ggcgctgtta cagtgatcca gcctagaccg gcaaaaacga tgtctgtttt
     5821 ttctttcaca gaaaactcaa aacgaacaag ctccggaaat gcctcgactt catcaggacg
     5881 aggaggctgt aataatccac ctacatgttt ttgataaaat tcgtctgctt tttccagttt
     5941 cgttcgatga atatttacat catttgacac ataagcaata aatgaagcac gattgccgct
     6001 gataaaatca aatcttgcaa gtccgcccaa gaaaagtgtt tgcccttcat tcagctgata
     6061 aactttaggt ttgatttctt ttaacggtgc aatgatccgc aaatcttttt tacctaagta
     6121 gtgcgccatt tgatggcgat ggataattcc cggtgtatca atcaaaaaat gcccgtcatc
     6181 taaaggaatc tcgattttat ccaaggtcgt tcctggaaac tgcgaagttg tgattacatc
     6241 ttgtactccc gctgtatttt tgatgatttg attgataagc gtcgattttc cgacatttgt
     6301 tactccgacg atatagacat cccgtccatc gcggtattct tcgatttttt ctaatagttc
     6361 cgtcatctca tgagatttct tagcactagt taataataca tctactggac gaattcctgc
     6421 ttcgtaagca cgttccttca tccattgtgt taatttagtt tttttcaatg atttaggtaa
     6481 tatatccact ttgttcccaa ctaaaagtac tggattgtca ccgacaaaac ggtgtagtcc
     6541 tgggattagt gaaccattga agtcaaaaat atccaccaca ttgacgatca atgcatcttt
     6601 ttgccctaat tcattcagta agcgcaaaaa gtcgtcatcc gtcaatgaga catcttgggt
     6661 ctcattataa tggcgtaatc tgaaacaacg ctgacaatag acttccccgg aagctaaacc
     6721 tttttccaaa gccgtttgcg gcgtgtatcc taattcaccg ggtttgtttg tttgtataac
     6781 ggcaccacag ccgatacatt gtaattcttc tgtcattcga ttccacctcg ccagcccata
     6841 tcagggtgtt ttgctaatag ataggccata attctttttt cccaaaaacg attaaattgt
     6901 gtcttccaac tatctgttgc gataatgggt tttacaagga tgctacgtat ccctgcacga
     6961 ttggctccac gtatatcagt cataatctga tcgcctacca ttactatctc atcatctgct
     7021 aaatccaatt gtttctttgc tatgttgatc cctcttgcta aaggtttcat tgctcgtgca
     7081 acatacaaaa gatcaaattt ttcaatcgct cgcgcgacac gttttgaatt attgtttgaa
     7141 acaacaacaa cagggattcc tgcatttttc atttctaaaa tccaagtaag caactcttct
     7201 gttccatctg gattattcca tgcaatcaac gtattgtcta aatcggtcaa tactgctttg
     7261 atgcctagat tctttaattg ggcaggtgta atctgataga tggcttctac catccatgtt
     7321 ggtttgtaat ttgaaaacat gctgtctcct catctgcaaa aaatgaagga gcacataaag
     7381 tactcctcaa ccccgtatta tactgcatat cagcaaaaaa aactacctct atactcgttc
     7441 gtctttcaga aaaagcattt ctttacaaaa tgaccaaaaa taaagattga tcacagaaaa
     7501 atttagaaac aaaaaacaac taatattggc ggaacctctt gtatcgtttt tccagtaatt
     7561 tatgctgatc taatcgaaca agttccgtca atctttcaat caacgctttt ttcataatgt
     7621 gtgcgacttt atatcgtggc aactgctcgc cgttgaacgt ttcaggaata actcgatcaa
     7681 cgattcctaa ctcgcgtagt tctggcgcag taattttcat caactcagct gcttctgcgg
     7741 cgcgactacc gtctttccac aaaatcgacg caaatccttc tggagaaagc acagcataaa
     7801 tcgtttcttc cagcatccag acttcatccg ctaaacctag agcaagggca ccgccacttc
     7861 caccttcacc aatgatgatg gcgatgatcg gtacctttaa atcagacatt tcgagcagat
     7921 ttctggcaat agcctctcct tcgccgcgct cttctgctcc tacaccgcaa tatgcacctg
     7981 ctgtattgat caaggtaatc accggtctat tgaacttttc cgcttgtttc atcagtcgta
     8041 aagcttttcg atagccttca ggatggggag aaccaaaatt tctgcggata ttttcctgca
     8101 gatcccgtcc tttttgaata ccgatcactg ttaccggttt gccatctaat cgagcgattc
     8161 caccaacaat cgcttcgtca tctgcaaata aacgatcacc atggcattcc tcgaaagaat
     8221 cgaagatctc ttcaaaaaaa tccaagcttg tccatcgttc tttagctctt gctaactgaa
     8281 cgacatcatg tgctgtctta tccacgatct atccatcctt tctgtgtatg caattcaaga
     8341 agcgtatgga ttttctgttt caattccatg cggggaacaa tctgatcgac aaaaccatga
     8401 gataataaga attcagcctt ttggaaatct tctggaagtt cctgcttgat cgtctgttcg
     8461 atgacgcgtc ttccagcaaa accgatcaag ctttggggtt ctgctagaat aatatcgcct
     8521 tccattgcaa aactggcagt tactcctccg gtcgtcgggt ccgttaaaac agttagataa
     8581 aaaagacctt cattgctatg ccgtttgaca gcagcagaga tttttgccat ctgcattaga
     8641 gaaaagattc cttcttgcat ccgtgcgcca cctgaagctg tgaacaagac gactggaaga
     8701 ctttcctttg ttgctcgttc gaataatctg gtgatctttt ctccgacaat cgttcccata
     8761 cttcccatga taaaattagg atccatcact ccaataatga agggaatccc ctgaatggtt
     8821 gctttaccag tgaggactgc ttcatgaagt cctgttttat cttgcatttt tttaattttt
     8881 tccgtatatt cgggaaatgc tagcggatct ttggttacaa gatccgtatc ccattcttca
     8941 aaactttttt catcaataat caaagctaga cgctgccatg caccgattcg aaaagcgtat
     9001 ccgcaatgag ggcatacttt ttcttcaccg atgtctttcg tataaaggat atgtttgcaa
     9061 tttggacact ttgcccacat gttatcagga acagatggcg agttagcagg tttgctctct
     9121 ctattaggat tgatccgaat atatttcttt ttcttaaaca gagccatcta tgcgcctcct
     9181 atctccgttt ctggtgtcca attcggtaaa aattcttctt gtaaaaatgc tgtgctgtaa
     9241 tcacctgcta tgactgcagg atgactaatc aaatccatct gaaattctgc gttcgtgatg
     9301 attccttctg tgataaattc acttaaggct cgctgcatct tcattaatgc ttcaaaacgc
     9361 gtatttccat ggacgattat ttttgcaatc attgaatcat aatatggcgg gatcgtatat
     9421 cccgggtaaa ctgcactgtc tactcgcagt cccatcccac ctgctggaag cagtaagttt
     9481 tggatcttcc ctggagaagg agcaaaatgg aaagcaggat tttctgcatt aatccggcat
     9541 tcgattgcgt gtccttggaa tactacttct tcttgacaca cattcaaagg ctcaccggct
     9601 gctatctcca cctgtttttt tactaaatcg attcctgtga ccatttctgt tactggatgt
     9661 tccacttgga ttcgagtatt catttccatg aaataaaaat cgccttcttc atccatcaga
     9721 aactcgatcg ttccggcatt ttcataattg actgctttag ctgctcgaac agcagcgtct
     9781 cctagtgctt ctctttttgt ttgtgagatc actacagaag gagattcttc cagtactttt
     9841 tgattgttgc gttgtaaaga acaatctcgc tcgcctaaat gaatgacatg accaaatcga
     9901 tcacctaaga tctgaacttc tatatggcga gcaggataaa tgattttttc caaatacata
     9961 tcatcatttc caaatgccgc tgctgcttct tgctgagctg aagaaaaatg ctgtgggagc
    10021 tcttcttttg attgaacttt ccgaatgcct ttcccgccac cacctgcagc tgctttcagc
    10081 atcactggat aaccaattct gtctgctatt tccaatgctt cttctactgt agataataca
    10141 ccagtacttc ctggaatgac tgggacattt gcttcttgca tcaattgacg cgcattgatc
    10201 ttatttccca ttgcatcgat cgttgcactt ttaggaccaa taaatgtgat attgcactct
    10261 tcacacatgg aggcaaatcg gctattttca gataaaaaac caaatcctgg atgaattgct
    10321 tcggcttttg tcacgattgc agcgctcagt acttcctgaa cattcagata tgaatcaacg
    10381 gcttttgccg ggccaatgca gattgcttca tctgctaact gtgtatgaag tgcttcggca
    10441 tctgcttctg aatatatcgc tacggtttgg atccctagtt cgcggcatgc acgaatgata
    10501 cgtacagcga tttcaccgcg gttagctatc aatactttag aaaacatatt tcacctatcc
    10561 aatcataaag gttaattccg cttcggctac ttttttaccg tctactttag cgattccttt
    10621 gcctattccg gctgaagctt tcactttcaa tatctcaact tcaaggtata aggtatctcc
    10681 cggtgtaact ttcttacgga attttgcttt gtccaatccg ccgaaataag ccgtctttcc
    10741 cttgaattgt tcaagagaaa gtaatgcaac tgctcctgct tgagccattg cttcaacaat
    10801 caatacccct ggcataacag gttcttctgg aaaatgtcct tgaaaaaagg gctcattgat
    10861 cgttacattt ttcttagcaa cgatccgttc tccttcaacc atttcttcca ctcgatcaat
    10921 caaaagcatt ggataacgat gtggaatgat ttcttttatt tcttgtatgt tcattgtttt
    10981 ccctctttcc tgctttgctt atttttgctg aatcctaaag agtggctgtc catattcgac
    11041 aactgactcg ttttctacaa ggatttctgt gatcactccg tcggcctcac tagttatctc
    11101 attcatcagt ttcatcgctt caataatgca gataacttct cctttggcaa cttgatcacc
    11161 tacttttttg aaagctggtt tgtcgggcgc tggttgcaga taaacgattc ccacgattgg
    11221 cgaaacaacc tcgattccat caaatactgc cgattctttt tctttcatta tttctggagc
    11281 agttgttgct tgttcaggta tgacattgac tggctccgaa actggcaacg tttcgtttgt
    11341 ttgaaccata ttcatgcctc gacttgtttt atttttattc atatacaatt caaattgacc
    11401 ttcacgcaaa tcgaattccg ttagcgagga ttgatcaaat tgactgacta attctctgat
    11461 ttcattgata tccaaaatta ttcctcccag cgtttcatgc acagaaccgc attatgtccg
    11521 ccaaacccta atgaattact cattgcataa gtaaaacttg caggtttgct ttcgtttttg
    11581 acaactgtca agtcaatctc aggatcttgt ttttctacat tgatcgtagg tgggataaat
    11641 tgatgttcta atgccagcaa agttgccaag gcttcgatcc cgccggctgc tcccaataaa
    11701 tgtccagtca tcgatttcgt gctgctgacg taagtatctc tgaaattttc acctaatgcg
    11761 atttggattg cttttgcttc tgcctggtca ttggctggtg tacttgttcc gtgagcattg
    11821 atatatccga cttgttccgg ctgtacacca gcttcttcca tcgcaagctt cattgcttta
    11881 gatgcaccag aaccatctgg agtaggagct gtcatatggt aagcgtcaca gtttgcaccg
    11941 tatccaacga tttctccata gattttggca ccgcgagctt gtgcatgttc taaagattca
    12001 agaactagaa cgcctgctcc ttcacccatc acaaaaccat tccgattttc gtcaaacgga
    12061 atcgaaccac gattaggatc agttgattct gagagtgctg ttaaagctgc aaatccagca
    12121 atccccatct cacaaatcgt tgcttctgtt cctccagcaa gaatgacatc agaatagcca
    12181 tgtttgatgt tacggaatgc ttccccaatt gaattcgttc cgctagcaca agctgttacg
    12241 atagctgtac aaataccttt tgcacctaca cgcaaagcaa tatttccagc tgccatgttg
    12301 gcaatcgcca ttggtacgaa cattggagaa actcgttttg cacctttttc atgtagacga
    12361 ataacttggt tttcgattgt tggaagacca ccgatcccag atccgatgat gacgccaaac
    12421 cggtcttgat tcattttttc tttatctaaa ccgctcatct ccattgcttc taaagcagcg
    12481 tagattccat agatcgaaaa catatccatt cgtttgctat cttttttaat aaaatattta
    12541 tcgtaaggga aatctttgac ttcacccgct aaggtaatcc ctgtttctga agcatcaaat
    12601 ttagtgatcg gtgcgatgcc attcgtcccc tcttttaagc tattcaaaaa gctgtctgcg
    12661 tcatttccaa taggtgaagt gactccatag ccagtaatta ccactcgatt catttgtttg
    12721 ctctcctttt cttatccgtg catgaccaat ccgccatcga cattgatgac ttgtccggtg
    12781 atatatggat tttgtgctaa gaaaactgct gtttgtgcaa tatcttctac ttgtccgaaa
    12841 cgctgcatcg gaatttgctt ttccgcttgt tccttgactt tgtctgctaa aacttccgtc
    12901 atatcagttg tgatgaatcc tggcgcaatg gcattacatg taatgcctct agttgcagct
    12961 tctcgtgcca cagattttgt taatcctact acgccagctt tactagctgc ataatttgct
    13021 tgaccaatat ttccaatcaa cccagataca ctagagagat taatgatggc cccttctcgc
    13081 tgcttcatca tttttttcaa tacgtgctgc gtcatattga aggttccaat caaattgata
    13141 tccagacatt ttttgaaatc ttctgcatcc attcgcatca caagtttgtc atttgtgatt
    13201 ccggcattgt ttactaacac atggatcgat ccaagctttt cttctgcttc tttgatcatt
    13261 tgtcccgctt tctcataatc agagatgtca ccggaaactc ctacgcattt tacaccgaat
    13321 gcttcgattt cttctatctt ttcttttgga atctctccgc gaccattcaa aatgatattt
    13381 gctccagctt ttgcaaaagc taaggccatc gctttgccga ttcctcttgt actacctgtg
    13441 ataaatacat tttttccttt tacatccata acgttcctcc ttatagtatt gctattgttt
    13501 catcaaatgt ctgcttattc tcaacacgca gaactggtat tgttttatcg atttttttca
    13561 taaatccgct taatactttt ccggggccga cttcgatgac ttgttcgatg ccgattgttt
    13621 ttaacttatg gatactctca tagaaacgaa caggttgcat gacttgctgt tcaagtagtc
    13681 ccgcgattgt ttctttcttc attatttccg ttgttgtgtt gcttatgata ggaaaagaag
    13741 gttcagagaa ttgaatctga cttaggtcct tcgccagttt ttttgctgcc ggttctaaaa
    13801 tagctgtatg aaaaggcccg ctgacattta gagggatcat tcttttgaac cctttttctt
    13861 ttaatagtaa aacagcttcg tctactgctt tttcttcgcc accaatcacg atctgctgag
    13921 gtgtattata atttgcagga gatacgatgc cgtacttgct agcttcatga cagctttcct
    13981 cgatcgtttc aatcggcgca ttcatcactg caaccatttt cccgcttcca gctggggcag
    14041 cttctgtcat ataagcgcct ctttttgcta ctaacgcaac tgcttcagtg aaggatagcg
    14101 cattactagc tactaatgcc gaatattctc ctaaactcag tcctaaagct gcatctggga
    14161 tgattccatg ctccttcaat aagcgataat acgcgatgct gactgttaga atagctggct
    14221 gtgtatattg cgtctgatcc aaacgttcat tttctgtaaa gcaaagttca gccatatcgt
    14281 agccgagaat ctcacttgct tcatcaaatg tctgctttac aacggcttct tcatataaat
    14341 cttttcccat accttgatat tgtgcacctt ggccactaaa taagaatgct gttttcatgt
    14401 ttttctccaa tatatccaga atatatgttt gattcttcct attcaaaaag gccgagacat
    14461 tcctatttca ggaaatgtcc cagcccccaa cctaatttta atgattatgc agtttgtttt
    14521 tctacgtaag tcactaaatc ttgaaccgtt ttgattccgt cttctgtttc gattttcacg
    14581 tcaaattcat cttcaatttc attgatgatt tggaataaat ccaagctgtc agcttctaag
    14641 tcttcttgga tgtcagtagt caatttgatt tcttcagttt cttttcctaa ttcttctgcg
    14701 atgatttctt gaattttttc gaataccata ataatatatt cctccgatat tgtttgcttt
    14761 ttatttttat aatttaacag tctacaactc catgagaata cttccccatg ttaaacctcc
    14821 gccatagcct gtaaatatta ctttctgctg ttttcctaag gtgatctttc cactagaaac
    14881 tgcttcatcc aataagatag ggatgcttgc agcagatgta ttcccatatt tatccatatt
    14941 ggtcaggaac ttttcttgcg gcatctttac ttttcgggca attttctcaa taattctgag
    15001 gtttgcctga tgcaaaagca gataatcaac tggtttgtct ttcatgactt cttggatatt
    15061 gtttgcaacg tctcgtacgg caaaatcaaa aatatctctg ccagtcattt gcaaataata
    15121 cgaaccttct gaatcttctg aatagaacgg atttttattt tgctggtagc cagcggtcaa
    15181 tgagctactt ctggtaccgt cagctgctaa tttttctttc agcaattttt tctgtggatt
    15241 cgcttctaag agaatcccac ctgctccatc gccaaacaac acagcagtcg tacgatcaga
    15301 ccaatcgatt agcttagata gtgtttcacc accaatcacc agtcccttag ttgagccgca
    15361 gcggatcatt ttttcagcca tgcttaacgc ataaacaaat cctgagcacg cagcactgat
    15421 atcaaaagcg atagcatgag ttgctcccaa tttcccttgt acttgtgctg ctacagatgg
    15481 tgtcgtgtaa tcgggcgaca ttgttgctac taaaataaaa tccaaatctt tagggtttag
    15541 atttttctgt tttatcaatt tttccgccac taaaatgcat aaatcagaag tctcctgatt
    15601 tgttacacat cttcgttcac gaatacccgt ccttgacgaa atccattcat cagaggtatc
    15661 catcatcttg gaaagatcat tattagtaat aatcttttca ggaaggacgc tggctgttgc
    15721 agtaatcatt ccaaattgtt ccattaaata tcctcactta tagtcttgca agaagtcgtg
    15781 caagtttttc agtgcatcca gcaaggcatg ctgttcctcc gtagccattc cgtctaagat
    15841 gttcttgacc atttcacgat gaaaatgctg atggacccga aatagtaact ttcctttttt
    15901 cgttagtccc aactttacaa cgcggcggtc atcttcactg cggatacgtt cgacatagcc
    15961 tttttttact aacttgttaa tagcagtggt caaggtccca acagtaattg atagttcttt
    16021 ggctacttct gaagttgttt ttttcttgta catcccgatt gcctcgatcg tgtgcatctc
    16081 tgtgattgac agatctttga attgagattt tttcagctca gactcttcga ttgtcaaaat
    16141 atcattgaag acactaacaa gataatcatt tactgttttt aaatctggtt ccatgttcca
    16201 ccgtccacta attagtttga ttatcaaatc ttttgatgtt caaactatat cttaactttt
    16261 ttcaaaatgc aaccttaaaa gatcattttt ttactatatg agcttttttt
//
LOCUS       DAZUKZ010000052        16121 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000052 DAZUKZ010000000
VERSION     DAZUKZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.052"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1421)
                     /gene="trmFO"
                     /locus_tag="ACG8EF_001649"
     CDS             complement(45..1421)
                     /gene="trmFO"
                     /locus_tag="ACG8EF_001649"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFS4946959.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(1390..3468)
                     /gene="topA"
                     /locus_tag="ACG8EF_001650"
     CDS             complement(1390..3468)
                     /gene="topA"
                     /locus_tag="ACG8EF_001650"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFS4946960.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(3590..4447)
                     /gene="dprA"
                     /locus_tag="ACG8EF_001651"
     CDS             complement(3590..4447)
                     /gene="dprA"
                     /locus_tag="ACG8EF_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFS4946961.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(4503..5270)
                     /locus_tag="ACG8EF_001652"
     CDS             complement(4503..5270)
                     /locus_tag="ACG8EF_001652"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFS4946962.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(5263..6123)
                     /gene="ylqF"
                     /locus_tag="ACG8EF_001653"
     CDS             complement(5263..6123)
                     /gene="ylqF"
                     /locus_tag="ACG8EF_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFS4946963.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            6383..6781
                     /locus_tag="ACG8EF_001654"
     CDS             6383..6781
                     /locus_tag="ACG8EF_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4946964.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            7018..7560
                     /locus_tag="ACG8EF_001655"
     CDS             7018..7560
                     /locus_tag="ACG8EF_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS4946965.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(7643..8194)
                     /gene="lepB"
                     /locus_tag="ACG8EF_001656"
     CDS             complement(7643..8194)
                     /gene="lepB"
                     /locus_tag="ACG8EF_001656"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4946966.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(8346..8573)
                     /locus_tag="ACG8EF_001657"
     CDS             complement(8346..8573)
                     /locus_tag="ACG8EF_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFS4946967.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(8570..9088)
                     /gene="msrA"
                     /locus_tag="ACG8EF_001658"
     CDS             complement(8570..9088)
                     /gene="msrA"
                     /locus_tag="ACG8EF_001658"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4946968.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(9108..9821)
                     /locus_tag="ACG8EF_001659"
     CDS             complement(9108..9821)
                     /locus_tag="ACG8EF_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFS4946969.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(9824..10720)
                     /locus_tag="ACG8EF_001660"
     CDS             complement(9824..10720)
                     /locus_tag="ACG8EF_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4946970.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(10878..11720)
                     /locus_tag="ACG8EF_001661"
     CDS             complement(10878..11720)
                     /locus_tag="ACG8EF_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4946971.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            11852..12505
                     /locus_tag="ACG8EF_001662"
     CDS             11852..12505
                     /locus_tag="ACG8EF_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFS4946972.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(12568..13095)
                     /locus_tag="ACG8EF_001663"
     CDS             complement(12568..13095)
                     /locus_tag="ACG8EF_001663"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS4946973.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(13115..14062)
                     /locus_tag="ACG8EF_001664"
     CDS             complement(13115..14062)
                     /locus_tag="ACG8EF_001664"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS4946974.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(14074..15957)
                     /locus_tag="ACG8EF_001665"
     CDS             complement(14074..15957)
                     /locus_tag="ACG8EF_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4946975.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
BASE COUNT         4246 a         3333 c         2819 g         5723 t
ORIGIN      
        1 aaaaagagcg agactatccg tctcaccctc ttcatggagt ttccttactc atttgggatt
       61 gcagtttttt ctaattctgt ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt
      121 gcttcatatc ttgctttttt gtctcgaatg cgctctggca gttcaggaaa cagaccatag
      181 ttggcattca tcggttggaa atgttttcct tccgcatgag taatgtagta agccatactt
      241 ccaatagctg tttcttgtgg aaactcgact ggttccattt cttttgctaa gcgtgcggcg
      301 ttgattccag caagtagtcc gctcgctgca ctttcaacat agccttctac accagtcatc
      361 tgacctgcaa agaacagatc ttcgcgtttc gttgattggt aagtcggttt cagcaattct
      421 ggcgaattca taaagctgtt tcggtgcatc acaccatagc gaacgaattc agcattttct
      481 aacccaggaa tcatccggaa aacacgtttt tgctcgcccc atttcaagtg agtctgaaag
      541 cccacgatgt tgtataaaga agctgcagca ttgtcttgac gcagctggat aacagcataa
      601 ggacgtttgc cagttttagg atcttccaat ccaactggtt tcataggtcc gaaaagcatc
      661 gttttgaatc ctcgctgtgc cattacttca attggcatac atccttcaaa atatttttct
      721 ttctcaaaag atttaagttc tgccacttcg gcattgatta gtgcctcatg gaaagctttg
      781 aattcctctt ctgtcatcgg acagttcaag taagctgctt ctcctttgtc atagcgggac
      841 ttcaaatata ctttatccat atcgatcgta gctttgtcta cgattggtgc agctgcatca
      901 tagaaataaa aaccatctga accattgaat gctttgatgc tttctgctaa tggatgagaa
      961 gtcaaaggtc ctgttgctac tacggtgatt ccttccggta tttcagtaat ttcttcgttt
     1021 atcactgtta ctaatggatg ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg
     1081 cggtcaacag ctaaggcacc accagctggc actgctgttt catctgccga agtgattact
     1141 accgaattca atcggcgcat ttcttctttc aataacccta ctgcattagc caaactgttt
     1201 cctcttaatg aattggaaca tactagttca gcaaaattac ttgtgtgatg cgcctcagtc
     1261 gatttgactg gacgcatttc atataaacgg acagggactc ctgcttgtgc gatctgccaa
     1321 gcagcttcac ttccggcaag ccctgctcca acaacattta ccattttcaa tgaaagaacc
     1381 tcttttctat tatttttgga tgtcttcttc gtaatctcca tttacacaga ctacttgttt
     1441 accgcctttg actttcttct cgaccagata tccgtcacat tttggacaat ttctgcccac
     1501 tggtttatcc caagaagtga attcacattc tgggaaacgg gaacaaccat agaatagtct
     1561 gtttttctta gattttcgtt caatgacttg tccttcatga catactgggc aagtcacacc
     1621 gatttctttt acgattggtt tcgtattccg acaatcagga aaattactgc aagcataaaa
     1681 cttcccatat ttccctaatt ttatcaccat cggatgtcca cagacatcac aatcgaatcc
     1741 tgctggttca tctttgattt gaattttttc gattttttct tccgcattcg tcagttcttt
     1801 ttcaaacggt ttatagaaac gatcaaccac tttgacccac gcttccgtgc cttcttcaat
     1861 tttatccaaa tctccttcca tttctgctgt gaaatggata tcgacgattt gagggaagaa
     1921 ttcacagatt aatgaattga caatttctcc tagttcagtc ggctcaaatc ttttttgtgt
     1981 caattttaca tagtagcgtc tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg
     2041 ccctaccccg ttttcttcta gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg
     2101 ttgagtaaaa tgttgttttg gttcaatatc aacagattgg actacatcgc cttcttcaag
     2161 ttctggaaga atattttctt tctcttcttt gccgtcatct ctaccttcca catacacttg
     2221 catgaatcct ttgaatttga tttttgaacc gttcgcaatg aagatgacac cattttgttc
     2281 taatgtcact ttcattgtat ctaaaatagc tggtgtcatc tgactagcca caaaacgtga
     2341 ccagatcaat gtataaagtt tcaattgatc tttatctaaa tattgtttga gttcatctgg
     2401 tgtacgaagc acactcgttg gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt
     2461 tttgatcttt cggtgatcat gtctagaaaa ttcttttcca tacgtatctt caataaatgc
     2521 tgcggcctct gcttttgctg tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat
     2581 caagccgact gttccttgtt tacccaaagt gattccttca tataattgct gtgctaccat
     2641 catggttttt cttgttcgaa agtttaactt acgggcagct tcttgttgca ggctacttgt
     2701 agtgaaagga ttcgcagggt ttcttttgcg ctctttcttt tctacttttt taacttcgaa
     2761 gtctgttcca tcaattctag cagtgacttc ttttacactt tgtgcatctg gtaatttctt
     2821 tttcttaccg tcgattcccc agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt
     2881 accgtcgatg ctccaatatt cttctggaac aaattctcgg atctcttttt cacggtcaat
     2941 aataattttt aaagcgacag attggacacg tcctgcactt aaccctttct tgaccttacg
     3001 ccagagaata ggactgatcg aataaccaac gattctgtct aaggtacgac gagcttgctg
     3061 tgcatctact aaatctacat cgatcgttct tggttcctta aaagctgctt tgactgcgtc
     3121 ttttgtgatt tcattgaaga ccacccgatt tttttctttc agatccaacc caagtaggta
     3181 cgctaaatgc caagcaatcg cttctccttc tctatccggg tcacttgcca agtaaacttt
     3241 ttgagctttt tttgctgcgg ctttcaggct tttgatgaca tcgccttttc cgcgtataga
     3301 aatataatgg ggttcataat tgttttcaaa atcgataccc attttacttt tcggtaaatc
     3361 tcttatatgg ccaacactgg ccattacttt atagtttcgt ccaagatatt tctcaattgt
     3421 cttggctttt gcaggtgatt ctacaatcac taaatattta tacgccatta ctgaatggac
     3481 tcctttccat tgtcgtgttc ctactattaa aagaggattc atacaaatcg tagctaatca
     3541 tatccattca gaaaacgttt gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa
     3601 attctgccaa gatatcttca cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat
     3661 gacatccggt agaatgttct gttaaaatat ttcctggaac agaaaaaact gttcttccaa
     3721 gatccaatgc ttgctgtgct gttatcagag agccgctttt tttcttggct tcaaagacac
     3781 atgttccttt agacaaagct gcgataatcc gatttctcat tggaaaacaa tgctttttcg
     3841 ggccggattg aggaggatat tcactcaaaa gaagatgatc atttgcaatc acctcttgca
     3901 agtcacgatt ttcagcagga tagacttgct ctatccctgt tccaatgact gcgattgtcc
     3961 gtccaccatg agagattgct gcttggtgac cgtatgtatc tatccctttc gcgcagccac
     4021 tgacgatcac atatccagct tctatcactg gttttagtaa ataataaact gtttttttgg
     4081 ctaaagcaga agtgtgtctt ccaccaacaa cgcttaacat gttacaagaa agtaaagata
     4141 gatcaccatt ataaaacagt acaattggcg gataggtcaa gtgaagcagc tgagggggat
     4201 atttgggatc aaagcaagtg ataaacgact gtttcgattg gattttgccc cagttagcgt
     4261 gaatttcctg ccaacttttg cgaaacagct cccgatgttt ggttatttta cctatcttaa
     4321 tgatgtcttc aaatccgatt tctttataag gatactgcat catccagtta gcaagtgccc
     4381 attttccttg actactgatt ccttttgtat aagcaagttt caataaattt tcttctattt
     4441 gatacatcat aaaaccgcct ttcgtctctc tttctaataa gatacgtaaa agcggctgtt
     4501 ttttattgta aatatttttt tacaggtgca aaacttttac gatggattgg cgtaattcct
     4561 tgtcgttcaa gtccttctaa gtgttccttt gttccataac ctgcattatg cgagaaacca
     4621 tatccaggat aaagcacgtc atattctttc atcaattggt ctcgatatac tttagcaatg
     4681 atacttgcag cagcaatcga cacagatcgt gcatcccctt tgattatttt ttcctgctga
     4741 ataggcaaat ccaaggtcat cgcatcgatc aacaaatagc ttggtacaat ggacaatttt
     4801 tctactgctt ggatcatcgc taatttactt gcttcataaa tattcacatc atcgattgtc
     4861 tgctgatcaa cgatcccgat cccgattgcc agggcttttt cttgaacttg ttgataaaga
     4921 agttctcgct ttttctctga taattgtttg gagtcattca gccctaatat gatctcattt
     4981 ttcggcaaga taactgcagc tgccacgaca ggtccagcta atggtcctct tcctacttca
     5041 tcaatgccgg caatccattg gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt
     5101 tcttcatatt tttccttaat taatgcttcc tttttcaatc gtcgttcaaa agaagccagt
     5161 gcttgctgca cgcctttgcg tggatcattt cttaatgatt ctagtgtagg atcatctatt
     5221 ctctgtatgc ttttcaatcg ttctttgata ctttggatgg attcagtcat cacttttttg
     5281 ctccatttct tcgcaacgat ccagtgtgta tcgtccaagt tttccttgac gaatatcatg
     5341 cacgactaat tcgctgccac gatcatagtc ttctttatac ccacgttttg ctgtaatatc
     5401 catcagtaat tccggtgcca atttttcttc ttgctcgatt gataaaccat aacgatcgct
     5461 gattcttcct gaataatatc gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa
     5521 atggacaagc tggtctttga tcgcgcctgt cagcgctagt tttttgccga tttcttcatc
     5581 ttcaaatttg ggccataaaa tccctggtgt gtctaacaat tccaactcat tacctaaacg
     5641 aagccactgc tgaccttttg ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc
     5701 tactagtcga ttcatcaacg tagatttccc aacatttgga atacctaaga ccattgcccg
     5761 aatagcgcga ggcttgatcc cccgagcagc atctcttgcc cgtttttctt tcaatgcttc
     5821 tttagccgca ggtactagac ctttaattcc tttactttct tgtgccgtga tagctaatgc
     5881 aagatggcct tgtttacgaa aatattcttg ccacagcttt gtctgttgtg gatctgccag
     5941 atccgcttta ttcaataaaa caaggcgagg tttttgctga atgatctgat ctagcattgg
     6001 attacgagaa gataaaggta atcgtgcgtc taccaattca aatacgatgt ccacatattt
     6061 cattttttca gaaacttctc gtcgggcttt tgccatatgt cccggaaacc attggatggt
     6121 cattgttctc tccattcctt tcataccgga atattccccg gctttacttg tttttttctt
     6181 tttcaatata aattcacttt tttattataa agaaattcca tctattagtt aagtgaaagt
     6241 gaaccagtcc tcattctatc acatcttcac ttttttgatg atgcaaattt ttattttggt
     6301 ttctatatat gaatctaaat ttttgacagc gtttcctaaa taagagatga tagacgaaca
     6361 aaacagaaag gagacgaaac aaatgaatag tatggttttt gttaattttc ctgttgctga
     6421 tgtcaatcgt tccgttgctt tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc
     6481 tactgaagaa gctggttcaa tggtatggga tgacactttt tggataatgc ttctctctca
     6541 tgatttttat aaaaaattta taaaagataa agaaatagct gataataaaa aaacaagtgg
     6601 tgctttgatt tcgtttagtg ttgaaaacgc ggatgaagta aaaaaatttg ctcaaattgc
     6661 aaaagaaaat ggtggtactt tccacgaagt agatatggga atgcctgaag aagaaatgtt
     6721 ttcattagaa gtccaagatc tagacggaaa tacattagaa cccgtatgga tgaagatgta
     6781 agcagataac taatctgacg ttttttataa caaaaaaaca aaggcggatg atacgataag
     6841 tattctccgc ctctattttt tttattcgta caatctcttt tttacagttt ataaaagcag
     6901 tcttctcacc ctttttttaa ttgttagatg ccagtgtatg taaatcacca atgtgtcatg
     6961 acacattggt gattttatgg tatcctttta ttattaactg acaaaggaga gaatattttg
     7021 aacactttga ttgcaaaaga cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg
     7081 acctctgctc ttttattagc ccttgatgtc atcttataca agatagcgat tggtcctgct
     7141 tattttcaaa tcacattcgg atttttaagt atggcgctta tgggttatct atatggaccg
     7201 atctgggctg gtttgcttga aatgttgtcg aacactttaa atttcacgat atttggttca
     7261 ggaacgtttc aagctgcttt tcttatcacg gctttccttg gtggatttat taatggctta
     7321 tttttatatc ataaaaagat aaacctgcct tccgtcatcg ctgcccaatt attgattatg
     7381 atcccagtca gcctttttat caattcttgg ctgatgtctg ttttgtttgg tgcagatttc
     7441 aaagtaattt ttctaggtag attcatcagg aatatgatcc aaatccctat tcaaataatg
     7501 gttgtttatt catttatcaa aactttagag gctagaaaag tgtttaaacg agctgtttga
     7561 ttttacgtac taaggtagta aactgatttt ggattaaaca ataaagcgtg cagtctcaac
     7621 agactgcacg ctttattgtt tctcactgga tccattttat ttcatcaagt ggataataga
     7681 caaatcttgc tttacctaga atatcagttt tattgatcgt gccaaacgaa cgactatctt
     7741 ttgaaactct tcgattatct ccaagtacaa aataattatc ttccggaagc ttggattgtc
     7801 ccgttagctc ttgtagtgta aagtccgtcg tataggatgc atttgcatga tctgatttca
     7861 ggtttttagt tagataaggt tccttaacta ctttcccatt gatcttcaat tgatcatttt
     7921 ggtagctgac attttctcct ggaagaccga ttactcgttt gatataaatc gtgccatctg
     7981 ctaattgaaa aacaacaata tcaaatcgtc tgatctccga aaatttttcc atcacaacca
     8041 tatcaccttg cttcaacacg ttttccattg aattgccttc cactggtacc gggatgaata
     8101 aaaatccccg caacatgaac gcaaggaata cagaaacaag taagtatttg aatacaagcc
     8161 agaagcgatt aatatagcgt tgtttttttg tcaatctcta tcaatccatc cctctttttt
     8221 tcattctttc tttattataa gaaaaatatc aaaaaaatgc gactagatag ctgaaataaa
     8281 aataattctt tcaattaggc tattggacaa aaaacttttt tgatcataaa gtacaaaact
     8341 atctcttata tgtcatgatt attttctaaa tatttttccc atgcttcgtc aaagatatcc
     8401 atggacggca aatagtcgac attcagttcc agataattag aaatttcttc gtagctagag
     8461 ctgtgcttag gaaattggat gtctttaccg gcttctgtag cgaaaacaga taaagcatca
     8521 tgtgtgccac cgcctcgcag tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt
     8581 tcctccaatt ttcttcgata aaggcagccc ggcgtgcatg gtttctttta aaattttcag
     8641 gatgcttttt ataatactct tgatggtctt cttctgctag ataaaatggt tgggctggtt
     8701 cgatttttgt aacgattggc tgttcgaaac gtccactttc agctaactgt ttcttacttt
     8761 tttcagcaat gatccgctgt tcttctgtca aataaaagat caccgggcga taattgtcac
     8821 cgcgatcctc gaattgtccg aaagcgtcag ttggatcagt ttgctgccag taaagttcta
     8881 ctaattgttc atatgaaatc aatgttggat catagataat ctccactgct tctgtatgtc
     8941 cagtcgtttg gctttttact tgttcataag tcggatctgg tacatgccct ccagtatatc
     9001 ctgacatcac tgtatggata ccaggaagcg tgtcgaaagg ctggatcata caccagaagc
     9061 agcctcctgc aaatattgct cgttccatta tgattccccc tctaatacta ctcatccgat
     9121 gattttttat tttcttttgt cttgctttca gtactgctgg tgctagtttt gttggtattt
     9181 gagttgtttg aagcatcaga ggtatctttt ggcaaatata gactaaaccg aatgtcatca
     9241 tttactaagt cgattttatc tgctttaata tacatcccgt tttgcattcg gaattgatcc
     9301 aacctaagca tgatagtcag atcatctgta ttgacttcga tccaatctgg taatttatag
     9361 tttctcttga tcatattcat cacatctttg attggcaaac tcaatgttcc gatagaaaga
     9421 cttctagctt ttagttggac atttccgttt tccatcacat acggatcaaa atataaatag
     9481 aaatcgatag gaaaacctag tacttcgaaa gtaccattga gtagtgcttc attttccaaa
     9541 taaaatttat attcaatatc actatctttt tgatactcac taaggaaaaa gctgatgatt
     9601 tgattgactt tttctttatt tgaattgatc gttaagacag gatctccttg tctttccgta
     9661 agagctggta tttctgggag ttctggttcc cgattagcaa aaatacggct gcctaaaaat
     9721 acggtcgaac caagtactac cccaagaagg ataaggaagg cgaatttcca gtaatttgtt
     9781 ttctttgttt tgtttgaaga tacgtcagat cttgttttca tgtttattga tcatcctcct
     9841 tctctaacca ttggttttgt gtctcgatca gctgatctct cactgcattt gccatcaatt
     9901 gataacctaa attatttgga tggaaatgat cttcttcata gagcacatta tttttgatgc
     9961 catttagatt ttctgtcgtg ctagttgttt gatctgttcc ttcttctgtg acacctactt
    10021 cctgattcaa tccttgataa agcaaatcat tgattgggat aaagtagcag ttcggcattt
    10081 tttttgtcac cgtttctgtt cctttgttcc aattgttaac aattgtctgc atgtcagtga
    10141 tctcaggaaa gttcaaataa aatggattgt agatgcctaa aatgtaaata ggtgcggttt
    10201 tattgaggga tcgaaattct tccaatagtt ctgtgacatt ttcctgatag tttttcagtg
    10261 gctttttaaa ggaattgacc gttaacccga ataaattatt ttggataact ttcatcaaat
    10321 cattaccgcc aacagtcagt gtgataaaat cagctgtttt gatattgttt cgaatttctt
    10381 cttttgtttt tactcgcttt aagatctggt cacttcgttc accatttaca ccatagtttt
    10441 ctgtttcaac cgcggtcaaa ttataacggt cttttagatc atttgcgaca aggggaacga
    10501 atcctccttg cttggtttga tctcctactc cttcagtcag cgaatctccg atagctatgt
    10561 agtgaagttt ttctttttta tttggatctt ctctcacagt agttactggt ttcattagtg
    10621 ggccagcttt cggtatagaa gcagctaaac ctgcatagat gatgatcgca attaataatg
    10681 ggaacaaaac gaggatcaaa tgtcgtttcg ttaatttcat tttttcacct cttgtattcg
    10741 attggttcta atgttatcaa aaattttatc aaaaaggtag tctttatgaa aaaagaaaac
    10801 aattattttc ctaattttat aaagaaaaag agaataagct gcaagccttt caggctgagg
    10861 gcttatcctc ttttcgacta atctacataa tacatgattg caaacgcgtc tttaccagta
    10921 tgcgtagcga taaccggatt cgtatgcagt acagggatat gcatgtccgg gaataattct
    10981 tgcaaaccct ctttgaattc ttgcgccaat tcttttgcat ccgcatgtga aataccgatt
    11041 tgtcgcacat ttggcaattc tttcagttca tttttcaaat tattaaacca tttatcgaac
    11101 gttttctttc ctcgcccctt agttacaggg attaattcag aatgggcgaa attcatcacc
    11161 acgcgtatgt tcaaaacact agacagcaaa ccagttgttc ggctgattct tccacctttt
    11221 accaagttat ctagtgtgga tattccgatg tataattttg tattgtcttg tacttttttg
    11281 acttcttcaa ggattttctc tactgtttta ccttcttttg ctaattcagc tgctcgaatt
    11341 acctgaaaag ataatccttg gtcagtggta tcactgtcga taaccgttac tttcgttttt
    11401 gttagattac tggcttgtct tgctgcttct actgttccac tcaagccttt tgtcatatgg
    11461 atcgaaatca cttcactgcc gtcttctcct agctcatcat acatcgagat gaattctcca
    11521 attggtggtt ggcttgtttt gggcagattt tttgctttcg ccatcatttc cataaatctt
    11581 tcgccgtcta gttggtcatc atcgggatat actgttccgt cgatcatgat ggatagcggc
    11641 atgacacgaa tgcctaaagc atcgcgtaca tttttttcca tcgtacatga agaatctgtt
    11701 acgattttta catttgtcat tattttctct ctctctttct ttaacaagat gccttattca
    11761 tgttaaacta catgtaagac aatttctatt tttagtataa cagacaaata atgatttttc
    11821 attaattaca cctagtaaag gagccgtttt catggaaaaa acgaaattta atcgaacgta
    11881 tgaaatatta aatgaagtgt ttaatgcagt cacccatgga attgggactg gattgagtat
    11941 cgcgggacta gtggttcttt tagtaaaagg tgcccacttg cattcctccc tccatattgt
    12001 ctcttatgct atctatggat cgatgatgat tcttttattt ttaacgtcta ctttgtttca
    12061 ttcattgatt tttacaaaag caaaaaaggt gttccaagta ttcgatcata gttcgatttt
    12121 tcttttgatt gcaggaagtt atactccttt ttgtttgctg agtatccgag gatggctagg
    12181 ttggacttta tttgtattaa tctggttgtt tgcgattctt ggcattatat ataaagcgtt
    12241 gacgttgcat aaacgggaga gagtatcaaa agtatctacg atcatctata tttttatggg
    12301 gtggctctgt gtcgtggctg ctgttcctct ttatcactct cttggaatga ctggtgttat
    12361 tttgatggta gcaggcgggc tttcttacac gataggcgca ttcttttata gcttgaattc
    12421 tatccgctat atgcatgttg tttggcattt gtttgttatg ttaggtgcag gttttatgta
    12481 cttctctgtt ttattcttta cttaataaaa aaaataaagc tatggatggg acagctctaa
    12541 ataacagcta gtctatccat agctttttta tatcgtcaat tcgcctgtcc cgctttttac
    12601 aggattctca ttacgctgat acgtttcaaa ttgatgcgga taacgatttt tttcatccat
    12661 ttctccttga ctagtattga tcagtgacca atcagaccag tcaacaggtg gaaaataagc
    12721 atctccatcg aatacatgat ggatgactgt acggtataat atcgtacaaa atggcagaaa
    12781 ttcttgataa acagctgacc ctccagcgat gaaaaagata ccgtctgttt ctttttccag
    12841 agcaagcacg tcttccacac tatgcatgac atgcacgcct tctgctttat aggaagaatc
    12901 tctcgtcaaa acaatcgtct gacgattcgg caacggacgg ctacccatcc cttcgaatgt
    12961 ttttcgtccc atcactagtg tatttgcttc tgtcatttgt ttgaagaatt tcagatcatt
    13021 tggcaaacgc caaggaagac ggtcttcttt tccaatcaat ccgttttcat cctgtgccca
    13081 aattgcagct aacattttct catcctttct gattctaaac agcgatagga gctttgatcg
    13141 ccggatgtgg gtcatatcct tctaattgga tatcttccat ctcaaaatca aatactgact
    13201 gcttttcctg attcaaaact aaagtcggaa atgcgcgtat ttctcggctc aattgttctt
    13261 tcatttgttc gatatgattg atataaagat gagcatctcc taatgtgtga acaaattcgc
    13321 caaccgctaa acctgtttca tgtgcaatta ggtgagtcag caacgcataa cttgcaatgt
    13381 tgaacggaac gcctagaaac acatccccac tgcgttgata caattggcag cttagccgtc
    13441 cctcttgaac ataaaattga aacatcgtat gacaaggagg caaagccatg ctaggaacat
    13501 cttccggatt ccatgcagaa acgatcaatc ttctagaatc aggagtcttt ttgattgctt
    13561 cgatcacttg tttgatttga tcgataaagc caccgtcttt tgtttcccag tgacgccatt
    13621 gatagccgta gatgtgtcca agatcgccgt atgtttcagc aaaggaatca tcggttagga
    13681 tcttctcgca aaaaagcttg ctctgctttt tatattcttc attgaattca ggatctgtca
    13741 aacatctatg gccaaaatca gtcatatctg gtccattata gtctggactt ttgatgtagc
    13801 gttcaaatgc ccattcatcc caaatatggt ttttattttg taatagatag cggatattcg
    13861 tatctccttt taaaaaccaa agcaattcgc ttttgatcaa tccaaaaggt actcgtttcg
    13921 ttgttaacaa aggaaaccct ttttgcagat caaaccgcat ttggtatccg aataagctta
    13981 atgtgcctgt gcctgttctg tcttctttac gatgcccttc ttccaacaat tttcttccta
    14041 agtcaagata cgcttgttcc atcctttttc ctcctaatct tctacccatt cactcaagta
    14101 ttcccaccgt gccatttttt cttcaagttg tgcttctgtt tcagatacgt cgttttgcaa
    14161 ttcttgtaat cgagtgaaat tatctccttg atgattcatc tcttcttgga gcaatgagat
    14221 tttttcttca agttctgcaa tctcatcttc aatcgtttcc cattcttttt gttccatgta
    14281 agataatttc ttcttttcag caggttcctt gctctgtatt tttggtttcg ctttttcagc
    14341 aggctgctgg gtctcttttt gcttcagtaa ataactggac atgtttccgt aataaagttc
    14401 ttgttttccg tttccttgga atactaataa cttatctgct accttgtcca agaaataacg
    14461 gtcatgggaa acagtgatca cagcacctgg aaaacttcta aaataatctt ccaaaatcgt
    14521 caatgttgct atgtccaaat cattggtcgg ttcatccaat aataaaacat taggttgctg
    14581 aatcagcagt ttcaacaaaa ataaccggcg tttttctccg cctgaaagtt tgcctatcag
    14641 tgtcccatgc ataaatcttg gaaataaaaa tcgttcaagt aattctgcta ctccgatttg
    14701 actgccatct gctgtttttg cttcttcagc agcttcttgt aagtaagcaa tcatccgttt
    14761 atctggtgcc atttcttcat tttcttgtgt ataatacgca agatgaaccg tttcaccgat
    14821 agacattgta ccgctatcca ggggaattct gccagctaga atattaagca atgtcgattt
    14881 tcccgcacca tttttaccag taatacctag tctttcccga gattgaatca ataaatctaa
    14941 atgttctagc agtgtttggt catttatacg gtaactgcca tctttgattt ccagtacttt
    15001 tttccctaaa cgttgtgtcg ctaggttcag ctctaaatca tcttcttggt ttacttggta
    15061 caagttctcc tttagatctt cgaaacggtt gatccgtgct tgctgcttgg tgccacgtgc
    15121 ctgtacgcca gccctcatcc aagctaattc ctgtttataa agccgcttgc gcttttcttc
    15181 ttgttctact tctacacgat cacgttctgc tttttctaag acgtaagctt cataatttcc
    15241 tttatactcg tataattttc caaatgaaag ttcgaagatt cggtttgcta ctctatctag
    15301 aaaataacga tcgtgggtaa ccatcaatag tgcgccacgg tattgtttca aatagttttc
    15361 caaccactcg atcgcttcat aatctaaatg gtttgttggt tcgtctagta atagtaaatc
    15421 gggttcatca atcaacactt gcgccagact aactcgtttt ttctgaccac ctgataaagt
    15481 actgattttt ttatctaatt cactgattcc taatttttgc aaaataatct ttgcgttggt
    15541 atctgtagtc caagcatctt ctttattcat ttgttcttct gctttagcat aacgtttttg
    15601 cacatcaggg tcttctccgt tttcccctag ttcgatcaat gcttcctcat atgctcgaac
    15661 agtttggatc aaggggctgt ttccttgaaa gactgcttgg agaacagtta attcatcgga
    15721 aaattcagct gcttgagaaa gataaccaat acggtaatca gttggataaa acactgtttg
    15781 ccgatcaccg tcgctgctat cgataccggc aataatattt aagagacttg ttttacccgt
    15841 cccgttcgtc ccaatcaacc cgatacggtc tttttcgtgg accaaaaaag aaatctggtc
    15901 aaacaaatct ttttcaccat aagttttttt gaggtcagtt acttttaatt ctttcaacat
    15961 gttccctcct tatcttggat attgtatcat aaaaatagtg aacttcagga aaattttttt
    16021 ggtatttgtt ctttttgtgt tctcaaaaaa agaacaggac cgattcgatc ctgttctgat
    16081 atgttttgtt ttttataaaa cttgaaaatt tttacctttt t
//
LOCUS       DAZUKZ010000053        16098 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000053 DAZUKZ010000000
VERSION     DAZUKZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.053"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..1475
                     /locus_tag="ACG8EF_001666"
     CDS             138..1475
                     /locus_tag="ACG8EF_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFS4946976.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="ACG8EF_001667"
     CDS             1523..3652
                     /locus_tag="ACG8EF_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFS4946977.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="ACG8EF_001668"
     CDS             3627..4316
                     /locus_tag="ACG8EF_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946978.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="ACG8EF_001669"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="ACG8EF_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="ACG8EF_001670"
     CDS             complement(5546..5740)
                     /locus_tag="ACG8EF_001670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946979.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="ACG8EF_001671"
     CDS             complement(5790..6230)
                     /locus_tag="ACG8EF_001671"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFS4946980.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="ACG8EF_001672"
     CDS             complement(6234..7079)
                     /locus_tag="ACG8EF_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFS4946981.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="ACG8EF_001673"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="ACG8EF_001673"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFS4946982.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="ACG8EF_001674"
     CDS             complement(8703..9650)
                     /locus_tag="ACG8EF_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4946983.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="ACG8EF_001675"
     CDS             complement(9873..10223)
                     /locus_tag="ACG8EF_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS4946984.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="ACG8EF_001676"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="ACG8EF_001676"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFS4946985.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="ACG8EF_001677"
     CDS             11646..11966
                     /locus_tag="ACG8EF_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS4946986.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="ACG8EF_001678"
     CDS             11988..12452
                     /locus_tag="ACG8EF_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4946987.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /gene="ytpR"
                     /locus_tag="ACG8EF_001679"
     CDS             12657..13262
                     /gene="ytpR"
                     /locus_tag="ACG8EF_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFS4946988.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="ACG8EF_001680"
     CDS             complement(13301..14590)
                     /locus_tag="ACG8EF_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFS4946989.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="ACG8EF_001681"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="ACG8EF_001681"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFS4946990.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15700..15825
                     /locus_tag="ACG8EF_001682"
     CDS             15700..15825
                     /locus_tag="ACG8EF_001682"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946991.1"
                     /translation="MIDEKKCLKNLLEDMDDIEENYLNGLGKQIYFRYLSYLKMK"
     gene            15822..>16098
                     /locus_tag="ACG8EF_001683"
     CDS             15822..>16098
                     /locus_tag="ACG8EF_001683"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFS4946992.1"
                     /translation="MRHSNFLAFLFAPNETHNLSDEFFKMFLKRYIDSNDDTKAAINY
                     FDALLNSYEDLIVYRENKNIDILLVSEKNKIVVCIENKILSSESGGQL"
BASE COUNT         5082 a         2952 c         3282 g         4782 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaagaaa actatctaaa tggactggga
    15781 aaacaaatat atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt
    15841 tagctttttt gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaatgt
    15901 ttttgaaaag gtatatagat tctaatgatg ataccaaagc tgctatcaat tattttgatg
    15961 ctttacttaa tagctatgaa gatttaattg tttacagaga aaataagaat attgatattt
    16021 tattggtatc ggaaaaaaat aaaatagtgg tgtgcatcga aaataaaatt ctgtcttcgg
    16081 agtctggagg gcaactta
//
LOCUS       DAZUKZ010000054        15995 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000054 DAZUKZ010000000
VERSION     DAZUKZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.054"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACG8EF_001684"
     CDS             226..894
                     /locus_tag="ACG8EF_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFS4946993.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="ACG8EF_001685"
     CDS             complement(1094..1480)
                     /locus_tag="ACG8EF_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4946994.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="ACG8EF_001686"
     CDS             complement(1452..2834)
                     /locus_tag="ACG8EF_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS4946995.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="ACG8EF_001687"
     CDS             complement(2990..4177)
                     /locus_tag="ACG8EF_001687"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFS4946996.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="ACG8EF_001688"
     CDS             complement(4184..5215)
                     /locus_tag="ACG8EF_001688"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4946997.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="ACG8EF_001689"
     CDS             complement(5231..6685)
                     /locus_tag="ACG8EF_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFS4946998.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="ACG8EF_001690"
     CDS             6860..7765
                     /locus_tag="ACG8EF_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFS4946999.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="ACG8EF_001691"
     CDS             7856..8533
                     /locus_tag="ACG8EF_001691"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS4947000.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="ACG8EF_001692"
     CDS             8530..8700
                     /locus_tag="ACG8EF_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947001.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="ACG8EF_001693"
     CDS             8814..9710
                     /locus_tag="ACG8EF_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4947002.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="ACG8EF_001694"
     CDS             complement(9765..11135)
                     /locus_tag="ACG8EF_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4947003.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="ACG8EF_001695"
     CDS             11373..12119
                     /locus_tag="ACG8EF_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4947004.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="ACG8EF_001696"
     CDS             12116..12955
                     /locus_tag="ACG8EF_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4947005.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="ACG8EF_001697"
     CDS             12956..13906
                     /locus_tag="ACG8EF_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS4947006.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="ACG8EF_001698"
     CDS             14047..14799
                     /locus_tag="ACG8EF_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947007.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="ACG8EF_001699"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="ACG8EF_001699"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFS4947008.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5192 a         3002 c         3221 g         4580 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtt
//
LOCUS       DAZUKZ010000055        15954 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000055 DAZUKZ010000000
VERSION     DAZUKZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.055"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..207
                     /locus_tag="ACG8EF_001700"
     CDS             <1..207
                     /locus_tag="ACG8EF_001700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947009.1"
                     /translation="YLLLESLFLQLLHNYFLCLKRSQKIIKAIMPFSTGIITSYKCIA
                     NVKPAVLIVLEKLLSEQLFYVRMN"
     gene            complement(514..880)
                     /gene="ssrA"
                     /locus_tag="ACG8EF_001701"
     tmRNA           complement(514..880)
                     /gene="ssrA"
                     /locus_tag="ACG8EF_001701"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1051..1824)
                     /locus_tag="ACG8EF_001702"
     CDS             complement(1051..1824)
                     /locus_tag="ACG8EF_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4947010.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1968..2318
                     /locus_tag="ACG8EF_001703"
     CDS             1968..2318
                     /locus_tag="ACG8EF_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFS4947011.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2299..3015
                     /locus_tag="ACG8EF_001704"
     CDS             2299..3015
                     /locus_tag="ACG8EF_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFS4947012.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3015..4463
                     /locus_tag="ACG8EF_001705"
     CDS             3015..4463
                     /locus_tag="ACG8EF_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4947013.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4533..5042)
                     /locus_tag="ACG8EF_001706"
     CDS             complement(4533..5042)
                     /locus_tag="ACG8EF_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947014.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5032..5757)
                     /locus_tag="ACG8EF_001707"
     CDS             complement(5032..5757)
                     /locus_tag="ACG8EF_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947015.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5980..8100)
                     /locus_tag="ACG8EF_001708"
     CDS             complement(5980..8100)
                     /locus_tag="ACG8EF_001708"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4947016.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8330..9508
                     /locus_tag="ACG8EF_001709"
     CDS             8330..9508
                     /locus_tag="ACG8EF_001709"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFS4947017.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9524..10282
                     /gene="aroD"
                     /locus_tag="ACG8EF_001710"
     CDS             9524..10282
                     /gene="aroD"
                     /locus_tag="ACG8EF_001710"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFS4947018.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10305..10790
                     /gene="ybaK"
                     /locus_tag="ACG8EF_001711"
     CDS             10305..10790
                     /gene="ybaK"
                     /locus_tag="ACG8EF_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFS4947019.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10861..12114)
                     /locus_tag="ACG8EF_001712"
     CDS             complement(10861..12114)
                     /locus_tag="ACG8EF_001712"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4947020.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12232..13581
                     /gene="gdhA"
                     /locus_tag="ACG8EF_001713"
     CDS             12232..13581
                     /gene="gdhA"
                     /locus_tag="ACG8EF_001713"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFS4947021.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13693..15039
                     /locus_tag="ACG8EF_001714"
     CDS             13693..15039
                     /locus_tag="ACG8EF_001714"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFS4947022.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15347..15733)
                     /locus_tag="ACG8EF_001715"
     CDS             complement(15347..15733)
                     /locus_tag="ACG8EF_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4947023.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(15782..>15954)
                     /locus_tag="ACG8EF_001716"
     CDS             complement(15782..>15954)
                     /locus_tag="ACG8EF_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFS4947024.1"
                     /translation="VETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQR
                     RFEGTLRYSLGY"
BASE COUNT         5215 a         2841 c         3196 g         4702 t
ORIGIN      
        1 tatctattat tggaatcctt atttctgcaa ttattacaca actacttctt gtgtttgaag
       61 agaagtcaaa aaataattaa agcaattatg ccttttagta caggaattat tacttcttac
      121 aaatgtatag cgaatgtgaa gcctgctgtt ttaattgttc tagaaaaact tttgagtgaa
      181 caattatttt atgttaggat gaattaattt ctctagagtt tataattaaa aaacttattt
      241 gaaggaacat gcttaaacca taattgtgtt tttccttcca tcagcataaa agttcacctc
      301 aaaaagagtg tacgaacaaa agttcttttt gtaaacctta aaatagattt tcctgaatac
      361 gtattgggga tttctaaatg tttgaatgtt tttttgaatg caaaatcaat gcaaaaaatt
      421 ttgtatttct ctacttttct ctccttgacc aaaataaaaa aacgttgatt taacaacgtt
      481 tttcatacat gtttatctgt ctcaaccata catatatgga gacggcggga gtcgaacccg
      541 cgtccaaaca catcgccact taaaaatcta cgttcatagt caactcattt aaggattcac
      601 tttacagctt gccgagtgac aggcattctg tatcgctagt ctgatgttct ctttttcaac
      661 ttacagacgg aaagttgaaa cgtatcccac taaagttagg acccttactc gagcacatgg
      721 gcgatgccga gaggatctac gctaactgtt tttaggcagc taaagcgtaa gagttgtttt
      781 cgtttttagc agttatattt aactgtaacg tttttacgta gacgtaacct acgaaacgca
      841 attcaagctc gactgtgcct gtcgaatccg taacgtcccc gttagctaac aagggttacg
      901 tgtatttttc atacaaaatt agtatagcat atattgggat aaattcaata aaaaagcgaa
      961 tcatatccca ttatttatat aataactgcc cctttcttaa gaaaaaataa gcgattttct
     1021 taagaaaaga ggtagcttca aaaaaagatc ttaaatcaga tcaaaatact tcaatgcgtg
     1081 gacaattccg ccttctgtat ttttctttgt gataaaggta gaaagttctt ttaatccgtc
     1141 ataggcgttg cccatagcga ttttgttgtc gcaagcttcc aacaaggcaa aatcattcgg
     1201 accatcgcca aatccaaaag taggaacttt ttccagctga agttcttttt gcagacgttt
     1261 gacacctgcc ccttttgaga ttcctttctt cactgtatcg atcgagtaag gaccattccg
     1321 ataaaaagtt agctcaggaa aatgttcgtg atagtattcg tcacctgatt gagataagat
     1381 cagcaacata tttactgtgt tttctttaaa gcctaaagga tcaatttccg gaacatctga
     1441 atgaatgaag tcgtaagcat tgatcattgt ttggctatga ccggtacacc agattttctg
     1501 atcgttatag taagaaagtt catgaccgtt caactgtaca tgatcataca ttttttgaca
     1561 ttcttctgaa gaaaatgttt gagaatagat ttcttcgccg tctactcgaa tgaaagaacc
     1621 attcatggtg accgcagaag taatgcccgc atcttccatg atagagtgga tttctgcttc
     1681 tgtacgtcct gtagcgatga cggggagaac attgttttct tttaatgcgt tcatagcttt
     1741 tgccacttcg ggtgtgattt ttgatttcgc atctagtaat gttccatcaa gatcaaagaa
     1801 agtgattgca cgataatttg acacaaataa aactcctttt ttattggaat aagtttatct
     1861 taacaaagaa tgaacaaaag acaaaccttt cgtgaagaat tcctgaacat attggttcag
     1921 tcaacaattt agagtatact ataagaagag aatttaggag ggaaagaatg atttttttca
     1981 gtattggtat cttattctta ataattggca gtctttttcg gattctgccc tcaaaaggaa
     2041 atctgccttt ttatgggtat cattcgcctc ttgccgcaaa aacagacgct cattggcggc
     2101 tggctcaaaa gacaagcgga aactggtttt ttcttatggg cttactgatg gcattgatcg
     2161 gctattattt aaagacatct gggcatacga attattttct gattgaaatg cttctgctag
     2221 tatttccaat tatgcctatt tttattatga cagaaaagaa attacagaaa tatgacctgg
     2281 aaactggagg gaacgataat gaatatttta atgattgaag ataatcaatc tgtttctgaa
     2341 atgatgcaga tgtttttctt aaatgaaggt tgggaagcaa ctttcaagta tgatggaaaa
     2401 gaagggctcg atgccttttt agaaaaccca caacattggg atatgattac actagacctg
     2461 aatttgccca cactggacgg tatgctagta ggaagagaga tccgcaaagt ttcaagtact
     2521 gtgccgatta tcatgctgac agctagagat tctgaaagcg atcaggtgat cggcttagag
     2581 atgggggcag atgattatgt cacaaaacct ttcagtcctc tgacgcttat tgctcgaatg
     2641 aaagctttgc atcgccgtag tgagttggca gaagcaagag aagaagcagc agtttcagat
     2701 gatcattttg atatcgagac tagtcatttc aagatgaata cgaaaacacg agaagcgtat
     2761 ctggacaacc ggccaattga aggactgacg cctaaagagt tcgatctatt gtttacttta
     2821 gcaaaaaaac cacgtcaagt attctctcgg gagcagttgc ttgaattagt ctgggattat
     2881 cagtactttg gtgatgaacg gacagtggac gcccatatca agaaattacg acaaaaaatc
     2941 gaaaaggtcg gaccacaagt catccaaact gtttggggag tcggctacaa attcgatgat
     3001 tctggtgttg cctaatgcgt tatttgtatc agcaactctt agctttttgc ggaatggtcg
     3061 cgttgatctt attgatcgtt ggagtttctt ttacacaact gacaaaacaa acgattgaag
     3121 aaaacaatta ccagcaactg tttggatatg ctgaatcagt tgaaaagaca acgcaaacct
     3181 atgccgatac gctcccgcag tttagtcaag atcaggcgtt tcaaaatgca ttgctgctga
     3241 cagagcaagt actgacagaa caaaatgtga actttatatt tatcgataaa aacgaacgtg
     3301 tcatttatcc gactaataca gctttactga attttacctt aacagctgat caatggaaaa
     3361 atctgaggaa tggacgtcaa gaaaaattca catcgaataa aaatatttta ggacaagcac
     3421 aggcgacctc ttacgcactt gtgccattta atttgaatca tgaattctat ggagcacttg
     3481 tagttagtca acctgcgcga aatatcgata acagcgttcg tccaattaca ctgaacctgt
     3541 tcaaaggatt tattttttcc agtatcgttg cagttattgc cagctatgct tttgcagcat
     3601 tccaagttaa gcgtatcaat cggttaagga atgcgacgaa agaagtgacg aatggtaatt
     3661 ttgatgtaca gctccccgta catgacaaag atgaatttga tgagttagca gatgatttca
     3721 ataaaatgac gaattcattg aaagaatctc aagcagaaat tgaagaacaa gaaaatcgtc
     3781 gaagacaatt tatggcagat gcttctcacg agatgagaac acctcttacg acaatcaacg
     3841 gacttctgga agggttagaa tacaatgcaa tccctgaaaa tcagcgagaa aacgcgatta
     3901 aattgatgaa aaatgaaaca gagcgtctga tccgtttggt gaatgaaaac ttggattatg
     3961 aaaaaatccg tacgaatcaa atcagtatgg tcattaaaaa attcaatgga acagaaacat
     4021 tgaaaaatat tgttgcacag ttagaagcta aagcagaagc agcaggagat acattgacat
     4081 tgaaagccgt tgatgatatt gatgtttacg ctgattatga ccgatttgtc caaatcatgg
     4141 tcaatattat tcaaaacgcg atacagttta ctgaaaacgg ccaaatcacg attactttag
     4201 aaaaaggcta tcttgaaacg atcatcacga ttgaagatac gggaattgga atgtctgaac
     4261 aacaaatgaa aagtatctgg gatcgttatt ataaagtaga tccttcaaga aaaaatacaa
     4321 aatatggtga atcaggatta ggtttaccaa tcgtgcagca acttgtacgc ttgcataaag
     4381 gaaaattaga agtagaaagc gaactaggaa aaggaacaaa attcgctgtt cgtttgcctg
     4441 acgtagaaat caaaaaagag taaaaaacaa gcggtataaa agatcatgaa tcgatcttct
     4501 ataccgcttg tttttactat aaaatgattg atttagcccg cgggctctaa acgatcaata
     4561 taaggacttt gagcaatagt agcatcatga gtaaagagta agataccttg attttcttca
     4621 tgagctaaat aagaaagcaa gtggagtagc tcttggatct ccaatacact aagttcatca
     4681 aatgtatctt caataacaat attgtttcgc ttggaaagca aggcatgaag taattgcagc
     4741 tttacttttt caaaagacgt caactcagct gccgcacaat taagcaagga aggagagata
     4801 tcaagttttt cttgataggc caaacgatct tgtttgattt ctttttccgg tacatccaac
     4861 aggacatggt ctttaagatt cagataagga aacatcggcc aatttttttg gatgatcgca
     4921 aaattatcca gttttgcaac aagaacctct tttttcaagt gctccaatat tttatgctgt
     4981 ttttcgagag gacacaaaat aacggtcaag tgttcagcat gaatcaaata ttcaggtttc
     5041 attacgtcca ggcctcctac tttgcaaatg atggataaaa aaagtgaaag ggaatagact
     5101 aagaatgaga cagcatccac tgagcccaat aagagtttgg gcaaatccct taagtatatc
     5161 tgtgaaaaaa agatcatttt ggaaatcgat ggtaaaaaaa gtctgttgat ccatcggaat
     5221 tgtaagtccg cttaccgaag tcaagatatc acggatatct ggcagttcag tagtctttaa
     5281 gacaagaaaa ttgatccttt gaagaagcgc ttcataaact gactggaagc ttacgatcca
     5341 caataaaacc aagatacaac aaagaaagag aggaaagaac agctgccaga aaataaaaag
     5401 gaataatttg gttttagaaa gaccagtcag gcgccaattg atcaattcct gcttgttttg
     5461 cctgactgaa tagtaaaaga aaaagataaa aaggaaggca aatagaaaaa ataaactgaa
     5521 tatgcctttt atataaaatc ggctagctaa ttctaaagcc ggaagaagct ctgtagatgc
     5581 agaagcgcca ataaatgaaa gccgatcttg gacttgattg gctaaagccg attgcaaatg
     5641 gtaaagagtc aggcatgcta tcaataaaaa agcagaaaag cttgaaaata aacaatagag
     5701 tattgtctgc tttttgtaat aaattaatcg agcagtagca tagcggatta ttttcatact
     5761 tttcacctct ataaaaagta tagaaaatga atatgaataa agtatgaaat tcctttagtg
     5821 aaatcacgaa ttaaaaccag aaaatatttt gtaaaaagca agaggttgtg aagaagctgc
     5881 cctattctat taccgtagat gcaagctttt gaacactgtt tattcgaaaa gaggagcagc
     5941 gacaataatt ttgtcgctgc tccttttaaa tttattttgt cattcggaag aacttgtggt
     6001 cgttccgtga agttcttggt acgtttgcgt tttataaaga tcaacagttg attgtccgtt
     6061 gtgttcacta aagacactag ttgacttgtc accaagagat ttttcagctt cttttaattt
     6121 ctctaagcca ttcttgtagt cataatcact ggtatcaatt gggtctaatc cactattcgt
     6181 atagaaccga agaagatcac cattattgat ttggtcagaa acagctaact gtttatcaac
     6241 tttttctttc agtgaatcaa tttcattctg tacttcctca gtaggttcag taatagcttc
     6301 accagtttcg ttactataga tcgtaccacc gtagtaagta tattttggtg taacaaaatc
     6361 accatcacgg aaagaaacaa gctgttcatg atctttcgag aatagatctt gtcctaactg
     6421 aatataattt tttgtatcta cacctagtaa gtgaagtaat gtaggcaagg catcaacttg
     6481 tccgccgtaa gtgtgattga ttccgccctg tgtttgtcca gggatatgga tcatataagg
     6541 gacacgctgc atttgtgcat tgtcgtagtt tgtccaagta gaacttgttt ttccaactaa
     6601 ttcagctaga tttttatttc ttgaattcga gacaccgtaa tggtcaccgt aaaggacgat
     6661 gactgaatta tcatacaagc cgctttcttt caaataattg aagaattctt gtacggcggt
     6721 atctaaataa ttggctgtcg caaagtaacc gttgatcgtt tcatcatttg tattggctaa
     6781 cgggaatcct gcttcgtcat tggtgaattt tgaataagga taatggttag atacagcaat
     6841 gaattttgaa taaaatggtt gttgcagacg ttccaagtat tgtacggatt gttggaaaaa
     6901 tggtttatca tgcaacccgt attggaacga attgtcatca ttaacatcat aataagaaga
     6961 atcaaagaaa taatcatatc ctaaattctt gtatgtctcg tttcgattcc agaatgtacc
     7021 agcgttccca tggaataccg cactagtgta accttgtgtt tgacttaaaa tatcaggtgc
     7081 agcttggaaa gtattttttc cgcctaattg tgtgaacaag gagccttgat tcaagccaaa
     7141 caatgaattt tccatcagcg tttccgcatc gcttgttttt cctgctttta cttgatggaa
     7201 aaagttgtca aaactgaacg tgctgttgct gtggtaaaga ctattgatga aaggtgtaac
     7261 ttcatgctgg agaccattct catctgtcaa tttataatcg atcaggaatt gctggaagct
     7321 ttctaaatgg atataaatca cattttttcc tttagcgagc ccgaacaaat cgtcatttgg
     7381 tgctgcataa tgttctttta cataattttc tacttctttc atatcattgg gacttgcttc
     7441 tgcccgcact tggtttgtct ggtaagtttg aacaccgtca taaaccatga aagcattcaa
     7501 gccaagatat ttgaccaaat aatcacgaga gaatgttcga gtcagcaatt ctggacgatc
     7561 agcttccgcc ataaaaagat ttcctgagaa aatcataact gctaatgtag agatcgcaaa
     7621 agccattctt gctcggatcg ggcgagggtc caacttgatt ttttttgcaa tcagcagaac
     7681 agtcaagata ataaaatcga tccaatagat cacatcatat gggcgaaaca gtcgaatggc
     7741 actttctccc agaccactcg ctactttccc agcacctaaa atcgtgttga tcgtaatgaa
     7801 gtccgtgaat tctcgataat aagtgacatt cgaaaaaaca agcaatgtca aaagaaaata
     7861 aatgaccatc atcgtgatat aagcaactct agggcgtctg atataaagag caattccaag
     7921 taataacaat gtagttgcaa tagggttgat aaacaagata aagtattgaa tgccactttc
     7981 gattccgaga tggaaatcaa ctaaataggc aaaaatcgtt ttcaaccaaa gtaaaacgac
     8041 tagtaaagaa aaaaatccga gcctcttatt caaaaaggca ggtgttttta tacatttcaa
     8101 gataatcttc ctttctgcac ttcttaatca cagaagtgtc atgaaaccat gttacacagc
     8161 tcacttattc tcatttattt tactagtatg acaatatagt gtcaattgaa accagaagtt
     8221 tatgctgttt cattaaggtt ttctttaagc ttcatgaaaa aatgagcagt aagacaagtc
     8281 ctatgttttt tgttatactt gggaagagaa agctaaagca ggtgaaggaa tgaaagtaaa
     8341 agtaaaaaaa agagcaagca atcgattgaa aaaaggatat ccactgatcc aaaaagaaga
     8401 ccttgctcat ccccaaaaag aaaccacgca atgggtggaa ttagttgatt cgcaaaacca
     8461 atttatcgca aaaggatatc taggaaagca gaataaggga atcggttggg tattgagcca
     8521 gcagaacatc ccatttgatc aatctttttt tgaagaattt tttgtgaagt caaaagaaaa
     8581 aagaactttc tttttttcag atgattctac taccgctttt cgtatattca atggagaagg
     8641 agacggaatc ggcggtctga ttgttgatta ttatgcaggt tatgctgttt tttcttggta
     8701 taacgaaacg ttatatgcac atcgtgaatt attgattcaa gcttttcgtg aaatctaccc
     8761 ggaaatcgta ggaggctatg agaaaattcg atttgaatca gtaaatctgc ctgagagcca
     8821 gttcatttac ggaaaagagg caccggaacc cttgatcgta aaagaaaacg gagtaaaatt
     8881 tgccgtttat ttaaatgaag gattaatgac tgggatcttt ttagaccaaa aagaagtacg
     8941 gggatcatta gcagaaggtt tcgcttctgg gaaaacagtt ttgaacatgt ttagttatac
     9001 cggcgccttt tctgttgctg ccgcaatggg aggggcaatc gaaacaacaa gtgtcgatct
     9061 agcaaaacgc agtttaaaaa aaacacagga acagtttgaa gtgaatggtt tggatgtcga
     9121 tcagcaaaaa atcattgtta tggacgtatt cgagtatttc aaatacgcca aaagaaaaca
     9181 atttttatat gacatgattg tattggatcc accaagtttt gccagaaata agaaaaaagt
     9241 ctttcgcgta gcaaaagatt atggggaact ggtcaaggat agtctggata ttttagcaga
     9301 agacgggata cttattgcgt ctacaaatgc tgcgaatgtc tcgatagaac aattccaaac
     9361 aatgattgaa gaggaattcc atgccaaaaa tgtttctttt gaacaacttg ctttatatcg
     9421 tctgccaaaa gatttccaga cgacggatac ctttttagaa ggcaattatc tgaaagtgtt
     9481 tgtttatcaa attaaaaata accattagaa ggagcgatgc agaatggttt gggtaaaggg
     9541 attgaatata ggaagcggta gaccaaagat ttgtgcgcct cttatcgggg ccgtccgaga
     9601 ggaagtatta cgtgaagcag ttcttgcacg tgaagcaggg gccgatttag tcgaatggcg
     9661 agtcgatcat tatcgtgaag ttttttcttt tgatgaagta gcagaaacat tggttcttat
     9721 ccatgagatg cttgatggac tgccgttatt atttacgttt cgtaccttag ctgaaggcgg
     9781 agaacaagac atatcgatcc gtcagtatcg cgacctttat gaacatgtga tacacacagg
     9841 attagtagat atggtcgata tcgagttgtt caaagtagaa agtctaggaa aaagactgat
     9901 agaggaaatc aagtctttaa acattccttt aattatcagt agtcatgatt ttaaagaaac
     9961 accagcagac cctgtcttgc tttaccgatt gaatgttatg gaacattttg gagccgatat
    10021 cggaaaactg gcagtaactc ccaataatga acgagatgtt ttgcggttga tggagttgac
    10081 tagacgggca aatgcttttg tctccatgcc tattattacg atgtccatgg gaaatcttgg
    10141 taaaatttct cgtctggctg gcggaacgac gggttccgtt atgacatttg gttccttaag
    10201 cagcgattct acttctgctc caggacaatt accggtcaaa gcattgaagc aaattattca
    10261 attattaaat gaaccgttgt agaacaagtt tgaggtgata ttttttggca aagaaaaaaa
    10321 tagtaaaaac gaatgcaatc cgtatggtcg aacaaaaaaa gattccctat acagagcacg
    10381 aatatgagtg ggacgaaagc cacctaagtg cgtcatcggt cgctgaacag cttccagaaa
    10441 gccaatctcg tatatttaaa acactggttg ctgtgggcaa tgtgacgggg cctcttgttg
    10501 ctgtgatacc gggagaagcc gaattgaatc taaaaaaatt agcaaaagtt tcagggaata
    10561 aaaaagtaga aatgctccat ttaaaagatt tggaagcaac gaccggatac atacgtggtg
    10621 gctgctcgcc tataggtatg aaaaaactat ttcctactta tttagatcaa attgcagaaa
    10681 gctatgaaca aatcattgtc tcggctggca gaagagggct acagatggag ttagcacctc
    10741 aagatatctg tgcattaacg agcggacaat ttgcagatat aaaacaataa atcatacgta
    10801 taaaagaaag aaggaggccg ggatgttgtt tgtccagtct ccttctttct tttttataca
    10861 ttatgctctt tgcccggcat tcattcctgc tacatgccct gtagcgaaag ccgctgtgat
    10921 attaaagccg ccagtataac cattgacatc cagtaattct cccgcaaaat acagaccatc
    10981 aatgatttta ctctccaatg ttttaggata aatttccttt agagaaaccc ctccgccagt
    11041 tacaaatgat ttctcaatag gaaaagtttt gttgattgat agcttaaact ctttgcatag
    11101 ttgaacaaaa tcttgaattt gtttttgaga ggcttgttgt cctgtcagat gatccatttc
    11161 aagccgctca agaaagaaaa ctaacaatcg ctcaggcaat ataccatgcc aagcattgac
    11221 caagttcttt ttggttgctt tacttttttc tgtcaatata tgaataagct cttgtttggt
    11281 ttgtgtaggg aaacaatcca gagaaagtgt cactggttct cccgttttct ctaattcttt
    11341 attgacaaaa cttgaacaac gcagtgcagc cggaccagaa ataccgaaat gggtgaaaag
    11401 taaatccatc gtatgttcag tcaatacacg acctttttta tttaaaacgc gtaaagtgat
    11461 gtcttgtaaa gaaagccctt gaagtgtttt ctcctgaata tacggttcat ccgatatcaa
    11521 gggggactct gtcgcataaa gaggcgtcac cgtgtgaccc actcgcttca cgatcttgta
    11581 cccatctcca gtagaaccag tagatggata agtccgtcca ccagttgtta agataatggc
    11641 aggagcatgg aactcttcga attccgtgcg tataccgtaa attttatgtt ctttgtggat
    11701 caatttctct actcgagtag agaatagaag cgtcacatct agttctttca gacgattgag
    11761 caatgcttcg ataatcgttt ttgatttatt tgttacagga aacatccgtc catgatcctc
    11821 ttcttttaaa tgtacgcctt gtgattcaaa gaagttcatg atatcgaaat tattccattg
    11881 cgcaaacgta ctatataaaa actttccatt tccaggaata tgtgcaatca aatcatcaac
    11941 aggacgattg ttcgtaacat tgcaccgacc accacctgtc atcagtagtt tttttcctgc
    12001 ttttttgttt ttttcgatca agagtacgcg tgcgccttgt tcagctgcac tgatcgctgc
    12061 catcatcccg cttgttccag cgccgactac gatcacatca tacagttttt ccattccctc
    12121 acctcaagga aagtgtagca tatctgaaga aagaaaacag aaaaaaaatg ttttgagcca
    12181 aaagagaaaa tgagctaaaa tgagggaaga taagcaaagg agcgtggaac gatgacaaac
    12241 gcaacagaat atgttcaagc aattcaagaa aagctacatc aaaaggatca agggcaagaa
    12301 gaatttttac aagcaatcga tgaattcatg cccacggtta tgtctttttt ggacactcat
    12361 cccgaatata tcgaaaagaa tatcttagaa cttttaacgg aaccagaaag agttattcaa
    12421 tttcgagtgc cttggcaaga tgatgaagga aactggcgag taaatagagg gtaccgtatc
    12481 caatacaact cagctatcgg cccttataaa ggtggattgc gcttccatcc tagtgtcaat
    12541 ctaagtatct tgaaattttt ggcttttgaa cagattttca agaatagttt aaccggtctg
    12601 ccaatcggtg gaggaaaagg cggaagtgac ttcgatccta aaggaaaatc tgataatgag
    12661 atcatgcgtt tttgtcaaag tttcatgctg gaattgtcaa aacatatcgg cccatcgatt
    12721 gatgttcctg cgggagatat cggtgtagga gctagagaaa tcggctattt gttcggcgaa
    12781 tataaacgat tgaaaaaata tgatactggt gtgttgacag gtaaaccttt ggatttttgg
    12841 ggaagtaaga tacgtacgga agctactggt tatggattag tgtattatgt caaacatcta
    12901 ttgaacgaag aaaaagattc atttaatcaa aaaacagttt ttgtttccgg aagcgggaat
    12961 gtagccattt atgcaatcga aaaagtgcaa gagctaggcg ggaaagttgt tacatgttca
    13021 gattctagcg ggtacatcta tgatccagaa ggaatagatg tgaaactatt gaaagaagtc
    13081 aaagaaatca atagacaacg tttgacggaa tacgcaaacc aacgtccttc tgctatttat
    13141 catgctggag aatctgtttg gacattgaaa gaaaaagcgg atatcgcttt gccatgtgct
    13201 actcaaaatg aaatagacga aagcttggcc gaaatcttag tagaaaatgg gatcaaaatc
    13261 gtagcagaag gcgctaatat gccatgtaca gtaaaagctg tttctgttct tcatgaagca
    13321 ggcgtgtggt attgtccagg aaaagctgct aatgctggag gagtagcagt atctgcttta
    13381 gaaatgagtc agaacgtcca gcgattgact tgggaaagag agcaagtgga tgagcagctg
    13441 gatcaaatca tggaaacgat ttaccatacg tgtcgccaaa cagcagccga gtacggcgac
    13501 gaaaaaaatc tgcttcttgg agcaaatgtt gctggatttg aaaaagttgc caaagcaatg
    13561 gcagcagaag gtttagtttg aaaaatgaaa ctttcttgga aatattgtca gcattttgtt
    13621 tatttgcttg aaaaataata aaaaaaaacg taaagtatta cctgtagagt aaacctataa
    13681 ggaggagaac aaatggcaca cattcatttt gactattcaa aagtagcacc gtttgtcaac
    13741 gaacaagagt tagggtacat gcaaagccaa gtaacagcag cccacaacga attacgggaa
    13801 ggtactggcg caggaagcga cttccgcggc tggatcgatt taccaacgaa ctatgacaaa
    13861 gaagaatttg cacgtatcaa agaagcagct aaaaaaatcc aatctgattc tgaagtatta
    13921 gttgttatcg gtatcggtgg ttcttattta ggcgctcgtg cagcgatcga cttcttgaat
    13981 cacacattct tcaatttatt agatagcaac acaagaaaag caccacaaat cttctttgcc
    14041 ggaaattcaa tcagctcaac atatattgct gacttgatcg aagtaatcgg tgatcgcgac
    14101 ttttcagtaa acgttatttc aaaatctgga acaactactg aaccagcgat tgcgttccgt
    14161 gtattcaaag agcttcttgt taagaaatat ggacaagaag aagcaaacaa acgtatctac
    14221 gctacaacag ataaagcaaa aggtgcagtt aaagtagaag cagatgcaga aggttgggaa
    14281 actttcgtga ttcctgatga catcggcgga cgtttctctg tattaactgc tgttggtttg
    14341 ttaccaatcg ctgttagtgg tgcagatatc gatgcattga tgcaaggtgc cgctgatgca
    14401 agcaaagcat actcaagcga caaactagaa gaaaacgaag cttaccaata cgcagcaatg
    14461 cgcaacattc tttaccgtaa aggcaaagta actgagttgt tgatcaacta tgaaccagga
    14521 atgcaatact tctcagaatg gtggaaacaa ttgtatggtg aatctgaagg aaaagaccaa
    14581 aaaggcatct atccttcaag cgcaaacttc tcaactgatt tgcactcttt aggacaattc
    14641 atccaagaag gacgtcgcaa tatttttgaa acagttgtga aggttgaaaa acctcgtaaa
    14701 tcaatcacaa ttcctgaaca agcagaagat ttagacggtc taggctactt gcaaggaaaa
    14761 gaagtggact tcgtaaatac aaaagcattt gaaggaacat tgcttgctca tacagacggt
    14821 gacgtgccta acttgctagt caaaattcca gaaatggatg cttatacatt gggttataca
    14881 atgtacttct ttgaaattgc tgtaggtatt tcaggttact tgaatggaat caacccattc
    14941 gaccaaccag gtgtagaagc ttacaagaaa aacatgttcg cacttctagg caaaccaggc
    15001 tttgaagatc ttgctaaaga attgaacgaa cgcctgtaag ataagggttt ttcgctagat
    15061 ttaaataatc agtgatagaa acatgttcgc tttaaaatga aaactggtac ccatttaggt
    15121 actcaaacat actgcatcgt ttattcagaa taaggggctg tgacaagacg tttgtcacag
    15181 tcccttgttt gtatagaaat cgattgattg ccttgctctt tagtttgttt taggacttta
    15241 agaacagtga ggctgacttc gaaggttaat tctgtttttt tgttttctag aatcagtaag
    15301 caaaaaagca ctcacttcga gtgctttttt gcgatatatt cttagcttaa gataggccta
    15361 atttttcttt tacagatgag ggtaccttat cgctggtaat tacttttttc tctatgatcc
    15421 gcttttcgct tgctttcaac tgcaagatcg ttccttcggg gtaagcagaa ccattggcag
    15481 agtcaccaga agtaatgaca tcgaaatcta actgtctttt ttcaccgttt tcagcataag
    15541 catctacgtt gtatttaaaa cctcttccaa ttacttcccc attatctgct ttgatatcag
    15601 tttctgcagg cattggagct ttgactaccg cataataatc tttacctaca taagtagact
    15661 gatagtagtc ccaacctttc catcctataa aagcaatcaa tatgataacg atcaacccta
    15721 aaattttttt catgaaagat ctcctttttt atttttatta tacccgtggt gctagttaat
    15781 tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata
    15841 gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg
    15901 agttgaaatt ttgacaacct aatttttcta aggaagaaaa aacagtctca actg
//
LOCUS       DAZUKZ010000056        15847 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000056 DAZUKZ010000000
VERSION     DAZUKZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.056"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..827
                     /locus_tag="ACG8EF_001717"
     CDS             75..827
                     /locus_tag="ACG8EF_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4947025.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            824..1738
                     /gene="pfkB"
                     /locus_tag="ACG8EF_001718"
     CDS             824..1738
                     /gene="pfkB"
                     /locus_tag="ACG8EF_001718"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS4947026.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1756..3687
                     /locus_tag="ACG8EF_001719"
     CDS             1756..3687
                     /locus_tag="ACG8EF_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS4947027.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(3798..5357)
                     /locus_tag="ACG8EF_001720"
     CDS             complement(3798..5357)
                     /locus_tag="ACG8EF_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFS4947028.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5512..7746
                     /gene="pcrA"
                     /locus_tag="ACG8EF_001721"
     CDS             5512..7746
                     /gene="pcrA"
                     /locus_tag="ACG8EF_001721"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFS4947029.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7763..9799
                     /gene="ligA"
                     /locus_tag="ACG8EF_001722"
     CDS             7763..9799
                     /gene="ligA"
                     /locus_tag="ACG8EF_001722"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFS4947030.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9905..10210
                     /gene="gatC"
                     /locus_tag="ACG8EF_001723"
     CDS             9905..10210
                     /gene="gatC"
                     /locus_tag="ACG8EF_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFS4947031.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10210..11682
                     /gene="gatA"
                     /locus_tag="ACG8EF_001724"
     CDS             10210..11682
                     /gene="gatA"
                     /locus_tag="ACG8EF_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFS4947032.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11679..13109
                     /gene="gatB"
                     /locus_tag="ACG8EF_001725"
     CDS             11679..13109
                     /gene="gatB"
                     /locus_tag="ACG8EF_001725"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFS4947033.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13119..14174
                     /locus_tag="ACG8EF_001726"
     CDS             13119..14174
                     /locus_tag="ACG8EF_001726"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS4947034.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14285..15658
                     /gene="rlmD"
                     /locus_tag="ACG8EF_001727"
     CDS             14285..15658
                     /gene="rlmD"
                     /locus_tag="ACG8EF_001727"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS4947035.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15661..15789
                     /locus_tag="ACG8EF_001728"
     CDS             15661..15789
                     /locus_tag="ACG8EF_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS4947036.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         5389 a         2903 c         3481 g         4074 t
ORIGIN      
        1 tgaacgtttt tgactgattt atattgctag tagaaaaaaa atatgctatt atttgtattg
       61 aggtgatcgt gaaaatgctt acagaggaac gcagacaaaa aatcctagac ttgcttgaac
      121 agcagaaaat cgtgaaatca caagatcttg taaacctttt gaatgcttct gaatcaacga
      181 taagacgtga tttacaagag ctggagaata gtgggtttct agaaagagtt catggcggag
      241 ctaagaaaga gcaattattg aattttgagc agaatatgag tgaaaaatca ctcaaaaacg
      301 ttcacgaaaa acagcaaatc gccaaatcgg cagctgaaac cattaatgac gaagatgtca
      361 tttatttaga cgctggctct actacgttag aaatgattcc ttttctgcaa ggaaaacaga
      421 tcacagtagt gacaaattcc gttcaacatg cggcaaattt agttgatttg gagcttgcta
      481 ccattatttt aggtggaacg ataaagctgg caactaatgc tgttttagga tctaatgcga
      541 tagaacaact aaaaaatttt cattttaata aagcatttat gggaatgaac ggtgcccatc
      601 ttgaacaagg attcacgact ccggatccag aagaggcagc tgttaaacgt ttagccatga
      661 aaaattccga agagacattt gtattgattg atcataccaa attcaaccag ttgtccttca
      721 cctctgttgc acctttggaa gcagccacga tcataaccga gcgttgtcca ttagaattcg
      781 ttaatgaatt ccgtgaaaag acaacaatta aggaggcaat acaatgattt acacagtaac
      841 acttaatcca tcaattgatt atatcgtacg cgtagatggg ttaaaactag gcgatttgaa
      901 ccgaatgaag gaagacttca agttacctgg aggaaaaggg atcaatgttt cccgtatttt
      961 gaaacggatc gattctcaat ctacagcact agggttttta ggagggttca ctggcgaatt
     1021 tatttcgaag tggcttgaaa aagaacaaat ccagtctttg ttcacaaccg taaaagaaga
     1081 tacaagaatc aatatcaaac taaaatcaga cactgaaact gagatcaatg gcttaggccc
     1141 atctatttca gatgaagaaa tcgatgagtt gaagcaagca ttaggaacag taggacaagg
     1201 agatatcgtc gtattgtcag gaagcgcgcc agctagcctg cgaaaaggat tttatcaaga
     1261 actgattgat atcattcgag aaaaaggtgc agatttcgtg attgatacaa caggtgatga
     1321 cctaaaaaat gcacttgtta agaaaccgtt gttgatcaaa ccaaacaatc acgaattagc
     1381 tgagctttac aacacaacct tccaatctgt agaagaaatc attccgtatg ggaaaaaatt
     1441 actagctgaa ggggcacaga atgtgctgat ctcaatggct ggagacggtg ctttgctctt
     1501 cacggaacaa ggaacttata aaacgaatgt attagttcgc ccattgaaaa attcagtagg
     1561 tgcaggggat tcaatgattg ctggctttat tggggcttac agccgtacga aagatccaat
     1621 cgaagccttt aaatggggag ttgcttgcgg aagcgcaaca gctttttctg atgacttggc
     1681 agctcgcgaa ttgattgatg agttgctgcc agaagttgaa atcacaaaaa ttgattagtt
     1741 tcaaggagga taatcatgaa tatcaaggat ttaatggtca aagaagcgat gatcatggat
     1801 ttgcaagcga ctgataaaaa aggcgcaatc gatgaaatgg tccaaaaaat gtttgatgcc
     1861 ggccggattt ccgatatcga aacatataaa gaaggaattc tagcccgcga agcacaaact
     1921 tctacaggtc ttggtgatgg aatcgccatg cctcacgcga aaaacagtgc agttaaagaa
     1981 gcaacagttt tattcgcaaa aagccaaaaa ggagtagatt atgaagcctt agacggacag
     2041 ccaacttatc tgtttttcat gatcgcagct ccagaaggcg caaacgacac acatttgcaa
     2101 gcattagcag cactatctcg attgctgatt gatgcagatt tcgtaggaaa attaaaagaa
     2161 gcaaatacac cagaagaagt ccaagacttg ttccaaacag ctgaagagca aaaagaagca
     2221 gaagaaaaag cagaacaaga aacacaagct gcagaagctt ctgctcatcc agaaagaaaa
     2281 ttcgttgttg ccgttacagc ttgtccaaca gggatcgctc acacttacat ggcagaagat
     2341 gctttgaaga aaaaagcgaa agaaatgggt gtggagatca aagtcgaaac gaacggttca
     2401 gaaggaatca aaaaccgatt gactgctgaa gacatcgccc gtgcagaagg tgtaatcgtt
     2461 gcagcagaca aaaaagtaga gatgaatcgc tttgacggaa aagaactgat caatcgtcct
     2521 gtcagtgatg gtatccgtaa accagaagag ttgatcaatc tagcccttag tggtactgca
     2581 ccagtattcc atggtgatgg aaaagaaagc agttcagaag aaggaaatgc ggatggtact
     2641 atcggacaaa gaatttacaa agatttgatg aacggcgtat cacacatgtt acctttcgtt
     2701 attggtggag gaatcgctat tgcgctttct ttcatgatcg accaatttat tggcgtgcca
     2761 catgatcaat tatcaaatct agggaattac aatcaagctg ctagctggtt caaccaaatc
     2821 ggacaagctg catttggttt catgctgccg gtattagcag gatttattgc ttcaagtatc
     2881 ggtgaccgcc ctggtttgat cgttggattt gctgctggtg cattagctaa tacaggcggt
     2941 gcaggattct taggtgcact gattggtgga ttcttagctg gttatgtcat cgtattttta
     3001 cgtaaattat tcaagaactt accaaaatca ttggatggaa ttaaaacgat cctcttttat
     3061 ccagtgtttg gcttactgat tactggtttc ttgatgttgc tggtcaatgt ccctatgaaa
     3121 gcaatcaatg atgcattgaa cggtttcttg acaagtatga gtggttcgaa tgctgcttta
     3181 cttggtgcat tattagctgg aatgatggcg gcagatttag gcggtccaat caacaaagcc
     3241 gcttatgtgt ttggtacagc aactttagct acgactgttg caactggcgg aagtgtcgtg
     3301 atggcttctg ttatggctgg aggaatggta cctccattgg caattttcgt tgctacacgt
     3361 ttgtttaaga ataaattcag caaaacagat caagatgctg gtttgacgaa catcgtgatg
     3421 ggactatcgt ttgtcacgga aggatcgatt ccatttgcag cagctgaccc gattcgtgcc
     3481 attccaagtt ttattattgg ttctgcttta actggcggat tagtcggagc tttcggaatc
     3541 aaattactgg ctcctcacgg cgggattttc gttgtattct tactgagtca cccggtcatg
     3601 tatttggtgt ttattgctat cggagcagtc gtatctggtg tgatttatgg tttattgaga
     3661 aaagctcctg cagtagcaga agcatagaaa taaaaaagaa gaggctgggt tttcccagcc
     3721 tcttcttttt ttatagaatc atatagaatc aagtagtgta gatgaatagt tgttgtaaaa
     3781 tagtttttcc atgttcgtta aaatgattgc ttcaaatttg tgatagaaga tttagcctcg
     3841 atttcctgct tgattcttgg caagtttgct tcatcagtca agataaccaa aatatctcct
     3901 acattcatga tcgtatcacc gtgagtcaag atttcctgat ttcctctgcg tatagagatt
     3961 agcagcatat ttttaggcca attgaagtct cgtaccatta atccgtctaa agaactctct
     4021 gccgttactg gaaaatcaaa agttgtcctt gcacctttta gtgaggcggt ctgagtattt
     4081 ccaagtagac gttccaataa tgattcgtaa attggattac cgccgagaag gtcattgacg
     4141 atataagcaa caagagcagc caatcctaaa ggcatcagat gattaagaga gccaaccatt
     4201 tctgtcacta aaataattgc tgtcagcggt gcttttccaa tcgctgtgaa ataaccagcc
     4261 atggcaataa cgacaaaatc cttgatgtag attggatcca tcccgaaatc aaccataatg
     4321 tttccataaa agctaccgat taaagccccc aatgtcaata tcggaaggaa aatcccccct
     4381 ggaagacccc caccgtaaga aatcatggaa aaaacgaatc gaagaacaaa caaaccgata
     4441 atcataatga ttggcaagga agcatgtcca aaagctaaaa cgatttgatt gcctccgcct
     4501 agagtactag gccataaata accaattggc aaaatcagca agaaaggaac gaacccgtga
     4561 aaataagctg gtaaaggcaa tcggttgtaa aattttggca aagccagcaa gacattctga
     4621 taaataaaac ctaagatgcc tagaagtact cctaagatca ctaaggaacc ataatatttc
     4681 aacggcaaag aaggaaggtc acctagatat aaaacaggtc tcaagccgaa aaagtaaaga
     4741 gagacgaagt tagcagtgat tgctgaagca aatgatgtca gccaaacaag tggagaaaag
     4801 gagtgatgga tttcttctaa aacaaagagt aatcctgcga taggagcatt gaatgctgct
     4861 gcaagtccgc tacttgcacc gctagaaatt aaaatctttt cttctgaaga tggactccga
     4921 aacaattgtc cagctccttg tccaatggat gcgccaagct gtatagaagg tccttctcta
     4981 cctagaaaaa ggcccgcacc aactgaaagt acaccgccaa tgaacttttt ccataaaaca
     5041 gagaaccagt tcattgataa ttccccgcga agctgtccct ctacttgtgg aatcccgctt
     5101 cccttgatat ccggttcatt tttgaccaga taacccacga aaaatgctgc aaggatgcag
     5161 caaatagcta ggccaattaa gtaaatcggc tgttcccgac tcatttcata gatacttcga
     5221 actaaatcgc ttagcttttc gatagataat cgaaacaggc taacgacgat tcctgtcagc
     5281 agtccaacaa atattccttt tagaataaaa tatatctgag tcttatctaa tttcttgatt
     5341 tttccttttt tttccattca aacgcctcgc tttcttttaa tttagcgcga aatcaacgaa
     5401 ttgacaagga aaaatccata aaaagaaaca aatgattttg aactagctag tttaatgaaa
     5461 gccaagaaaa tgctataatt aacaagattg ataaaagaaa ggatgttgtt aatgtcatcc
     5521 aaagctgaat tattaaacgg gatgaacccc cgtcagaaag aagctgttct tcacactgac
     5581 ggaccattac tgctaatggc aggtgcagga agtggaaaga cgagagtgct gacacaccgc
     5641 attgcctatt tgattgagga aaaagaagtc aatccttgga acatcctagc gattactttt
     5701 accaataagg ctgcaaaaga aatgaaagaa cgtgtcaatg cgattctcgc atctggaggc
     5761 gaagatgtct gggtatcgac ttttcactcg atgtgtgtgc gtattttaag aagagatgtt
     5821 gattttatcg ggtataaccg gaactttaca atcatcgatt cttctgaaca gctgaccttg
     5881 atgaaacgga tactaaaaga actgaacatt gatccgaaaa aatatgatcc tcgaagtata
     5941 ttaggaacaa tctctcaagc aaaaaacagc ctgcagaccc ctcaggattt tgcaaaaatg
     6001 caaggcagtt attacgaaga gattgcggca aaatgttatg ctgcttacca aaaagagctt
     6061 caatataatc aatgtatgga ctttgacgac ctgatcatga atacgattcg attatttgaa
     6121 gaacatcctg attcactcac gtattatcaa aacaaattcc attatattca tgtagatgag
     6181 taccaagata caaatcacgc acagtatacg ctagtcaatt tattggcagg tagatttagg
     6241 aacctgtgcg tcgtgggaga tgcagatcaa agtatttacg gctggcgcgg tgcagatatg
     6301 cagaacatct tggattttga aaaagattat ccagatgctg cagtaatttt gctagaacaa
     6361 aactatcgct ccacgaaaaa catccttagc gcagcaaacc aagtcattga aaacaatagc
     6421 aatcgaaaac caaaaaactt gtggacagaa aacaaagaag gaaacaagat cacttactac
     6481 cgtgcagata atgaacgaga cgaaacccgt tttatcgtag accggatgca agaagagatt
     6541 cgaagcaatc atcggaatta cggcgacttt gccatcttgt accggacgaa cgcacaatct
     6601 cgtgtaatgg aagaaactct gttgaaagca aatattcctt ataaaatggt tggagggcac
     6661 aagttctacg atcgtaaaga aatcaaagat attttagcgt atttgaatgt gctggtaaat
     6721 cctcaagatt caatcagttt tgagcgtatc gttaattcgc ccaaacgagg catcggacca
     6781 ggatctatcg aaaaactacg cagttttgct tctcttcatg aatggccact gcttgaagca
     6841 gcgcaaaatg tggatctggc aaatatcggt ggaaaagcag gacagcaatt aggtgcattt
     6901 ggagagatga tccaagaagt cactcagatg atcccatact tgacagtgac agaattgacg
     6961 aaagaggttt tggatcgtag cggatatcta gaagacttaa aaatccaaaa tacattagaa
     7021 gcacaagcac gcatcgaaaa cttggaggaa tttttaacgg tcacacaaga attcgacaaa
     7081 caattcgaac agcaaaatga agaagacgct gatgcaccag aagaaaaact aacggtcttc
     7141 ttaaatgatc tggcacttgt ttccgacatt gacaatttag aagaagatgc ttcccaagtg
     7201 acattgatga ctttgcatgc agccaaagga ttagaatttc cagttgtatt cctgattggt
     7261 ctagaagagg gggtttttcc tttatctcga gcattgatgg aagaaagcga actggaggaa
     7321 gaacgccgtc tcgcatatgt ggggatcacg cgagcagaag aagcgttgta tttaacaaat
     7381 gctttctcca gaacattata tggacggacg caatataacc gaccttctcg ttttgtagaa
     7441 gaaatcgatc aggagttact ggaaatcgaa ggcatgcgtc ctacgcctaa aaaaacgcct
     7501 gtattcgcta aaaaaacagc atatagctat aaacagccag agacagcggt tgttccctct
     7561 aaaagtgcta ctggagggga aaataacagc tggaaacctg gagataaagt caaacacaaa
     7621 aaatggggtc taggaacagt cgtccgagta agcgggacgt cgaaggatct agaactagat
     7681 gtggctttcc caagtcaagg agtaaaacgt cttttggctg cctttgcgcc aattgaaaaa
     7741 gcttagtagt ggagggtaaa tgatggaaca tgaaatgacg attgacgaag ctgctaaacg
     7801 agcagaagaa cttcgtacaa gactgaacca atggtctcgc gaatactacg tagaagataa
     7861 accaacagtg gaagactatg tttatgataa agaatacgca gaattagtag caatcgagga
     7921 acaatatcct gatttgatca cgtctgattc tcccactcaa cgagtaggcg gaaaagtatt
     7981 agaaggtttt gaaaaagtca ctcatgatat tccgctttac agtttgaacg acgttttcag
     8041 caaagaagag ctgatagctt ttgatcaacg tgtacaaaaa gcagtgggac gtgtagttga
     8101 ctattgctgt gaactaaaaa tagatggact ttccgtttct ttgcgttatg aagatggaaa
     8161 tttcgtgaga ggtgcgactc gcggggatgg aactgtagga gaaaacatca ctgaaaacct
     8221 aaaaactgtc cggtcggtcc caatcaaatt aaaagaacca atgaacatcg aagtaagagg
     8281 cgaatgtttc atgccaaaac ggtcatttgt ccaattgaac caagacagag aagcagaggg
     8341 aaaagatatc tttgcgaatc ctcggaacgc cgcagctggt tctttgcgtc agttagattc
     8401 taaaatcaca gcgaaaagaa atctggacac ttttttatac acagtagctg atttcggtcc
     8461 tatgcaagca aaaacccagt atgatgcgct agaagagcta gaaaaaattg gattccacac
     8521 gaatcgggaa aaacgtctat gtcattcgat tgatgaagtg tggtcataca ttgaagaata
     8581 tcatgacaaa cgtgtggatt tgccctatga aatcgatgga atcgtcatca aagtgaatga
     8641 attctcgcta caagatcaac ttggttttac tatcaaagca ccgagatggg cagcagctta
     8701 caagtttccg ccagaagaag ttgaaacgct aatcgaaaac atcgaatgga cagtcggtcg
     8761 aacaggggta gtaacgccaa cagctatcat gacaccagtc cgagtagcag gaacgacagt
     8821 aagccgtgcc agcctgcata acggtgatta tattaagttg aaggatatcc gcttgaaaga
     8881 tacagttctg atttataaag cgggagacat catcccagaa gtttcgcaag tcgtgttaga
     8941 taaacgacct aaagacagcg aagaatacca attgcctaca cattgtccag tttgtggaag
     9001 tgaacttgtc catttggatg aagaagtagc cttacggtgc atcaatccta aatgccctgc
     9061 tcaaatgaaa gaaggtttga accactttgt ttcaagaaat gcgatgaata tcgatgggtt
     9121 ggggccgcgc gtactagaac agatgtatga taaaaaacta gtagcagatg ttgctgatct
     9181 ttacaaattg acagaagaag agttattgac gttggacaaa ataaaagaaa aatcagcaaa
     9241 taatattttg acagcaatcg acaacagcaa agataattcg gtcgagcggt tgattttcgg
     9301 gttaggaatc cgacatgtcg gagcgaaagc agcaaaaatt ttagcagaac atttcggtga
     9361 tctagaaaca ttgagtaaaa gtgactatga gtcgattatt gcgcttgata cgatcggtga
     9421 tatcattgcc gacagtgtag tcacttattt cagtaacgaa gaagtccatg aattgatgaa
     9481 tgaattaaaa caagcaggcg tcaatttcga atacaaaggg cttcgaaatg cccaattgca
     9541 agaagtcgag tctcctttta aagaaaaaac agtagtcctt actggtaaac taacacgatt
     9601 tactcgtgaa gaagcaaaag agacgatcga aaatcttggc gggaaagtga ctggtagcgt
     9661 atcgaaaaaa acggatatcg tggtagcagg agaagatgct ggcagcaagc tgaccaaagc
     9721 acaagaatta ggaatcgaag tttggacaga agatcaaatg gcagatgcat tggctaaaag
     9781 tcgctcagta gaagaatgaa aaatgagttt tttcagacaa atttcaataa acaatttcag
     9841 acacggcact cttgtgttaa aatagagagc aaagttttta actcgtttag aaagaagggg
     9901 tagtatggcg attagtgaag aacaagtgaa acacgttgcc aaattagcaa aacttgcgtt
     9961 cgcagatgaa gaacttgcaa gttttaccga ccaattaggt aaaatcatcg atatggttga
    10021 aatgctggaa gaagtagata cggaaggtgt tccatttacg tcgaatgtgg ttgaaacagt
    10081 aaacgtgatg cgacaagatc gggcgcaagc tggatggaac cgcgatgaat tattgaaaaa
    10141 tgtgccggaa acagaagacg gctttatcaa agtacccgca atcatcgata atggggaggc
    10201 tggcgcataa tgacagaatt acacaatcgt tctctagaag aactgcatca tatgcttgta
    10261 tcgaaagaaa tcactgtaca agatttgaca aaaacaacat ttgaacgaat caaagaaaca
    10321 gagcctgagg tcgaggcatt cattacattg aacgaagaaa aagcattgga acaggcaaaa
    10381 gctttagatg aaaaagggat tgatgaaagc aacgtccttg ctggtattcc aattggtatc
    10441 aaggataata tcgtaacaaa agatatcttg acaaccgctg catctaagat cctttacaat
    10501 tttgaaccaa tttatgatgc aacggtcatg gataaaatct atcaagcaga tatgatccct
    10561 gttggtaagc taaacatgga tgaatttgct atgggtggaa gtacagaaac ttcttatttc
    10621 aagaaaacaa aaaatgcttg ggacccaaca aaagtacctg gtggctcttc tggtggttct
    10681 gcagcagcag ttgcagctgg acaaattcca gtttctttag gtagtgatac tgggggaagt
    10741 atccgccaac cagcagcttt taacggcatc gtagggatga aacctactta tggccgtgtc
    10801 tcacgttttg gtttgattgc ctttgcttca tctcttgacc aaatcggacc aatgacccgt
    10861 acggtgaaag ataatgcatt agcattgaca gctatcagcg gttatgacga aaaagacggt
    10921 acttcttcag gcgtttcagt tcctgatttt gcagagggac tgacaggcga catcaaagga
    10981 atgaagattg ctttacctaa agaatactta ggcgaaggtg tggcccctgg cgtaaaagaa
    11041 gctgtattga aagcagcaga aacattccgt tcattaggtg ctgttgtgga agaagtcagc
    11101 ttgcctcatt caaaatacgg tgtggctgtt tattatatca ttgcctcatc agaagcaagt
    11161 tccaatttgc aacgattcga tggtatccgc tacggttatc gctctgaaaa tgtgaaatca
    11221 ttagatgatg tatatgtcaa ctcacgttca gaaggattcg gtgaagaagt aaaacgccgt
    11281 atcatgttag gtacattctc cttaagcgcg ggttattatg acgcttactt caaaaaagca
    11341 ggacaagtcc gtacattgat caaacgagat tttgaaaaag tctttgcaga ttatgacttg
    11401 atcatcgggc catcttcacc aacagttgca ttcggaatcg gtgagaacat taacgatcca
    11461 atcacaatgt acatgagtga catcttaacg atccctgtca acttggctgg acttccaggt
    11521 atgtctgtac cggcaggttt atcagaagga ttaccagttg gattgcagat catcggtaat
    11581 tattttgatg aaaaaacgat gtatcaagcg gcatatgcgt ttgaacaagc aacagaattc
    11641 cataaacaac aaccagctat tttaggaggg aaagacgaat gaattttgaa acagtcattg
    11701 gactagaagt ccacgtcgag ttaaaaacca actcaaaaat tttctctcca gcgccagctc
    11761 attttggtgc agaaccaaac agcaatacaa acgtgatcga ctggggttat ccaggcgtgc
    11821 tgccagtaat gaataaagct gcgctggaat tcgggatgaa agcagctttg gcattaaact
    11881 gtgaaatctc taaagatact cattttgacc ggaaaaatta cttttaccca gataatccaa
    11941 aagcatatca aatttcccaa tttgatcaac caatcggtca tgacggatgg atcgaaatcg
    12001 aagtagaagg taaaaagaaa aaaatccgga tcgaacgtgt ccatttagaa gaagatgctg
    12061 gaaaaaacat gcatggtatt ggcggttatt cttacgttga tttgaaccga caaggcacgc
    12121 ctttgatcga aatcgtttct gaagctgata tgcgttcacc agaagaagca tacgcttatc
    12181 tagaagcatt gcgatcaatt atccaattta cagaagtcag cgatgtgaaa atggaagaag
    12241 gctctatgcg ctgtgatgcc aatatttctc ttcgccctta tggacaagaa gagttcggta
    12301 cgaaagcaga attgaaaaac ttgaactctt tatctttcgt gaaaaaaggt ttagcttttg
    12361 aagaaaaacg tcaagcaaaa gttcttcttt caggaggaga aatccaacaa gaaacacgcc
    12421 gttttgacga aacaacgaat aaaacagttt tgatgcgtgt caaagaaggg tctagtgatt
    12481 atcgttactt cccagagcct gatatcccga aattcgtgat cgatgacgaa tggattcaaa
    12541 aagtaaaagc tagtttgcct gagatgccag cttctcgtcg cgaacgctat atccgtgagt
    12601 ttggacttcc agagtatgac gcaatggtat tgacacttac aaaagaaatg tctgattttt
    12661 ttgaagctgt tttggaaaat ggtgcagatg ccaagcaagc atctaactgg ttgatgggtg
    12721 aagtttctgc ttacttgaac agtgagaaac tggaattagc tgagacaaaa ttgacacctg
    12781 agaacttagc aggaatgatt cgtttgatcg ccgatggaac gatcagctca aaaatcgcca
    12841 aaaaagtctt ccgtgaattg atcacaaatg gcggagaagc ccaagaagta gttgaaaaaa
    12901 atggttgggt gcaattatct gatccgaaca aactactgcc aatcattaat gaaattttgg
    12961 ataacaacca acaatctgtc gatgatttca aaaatggaaa agatcgtgcg gttggcttcc
    13021 tagtcggtca aatcatgaaa gcaactaaag gacaagcaaa tcctggtgtc gtgaataagc
    13081 tacttaaaga tgaattagcg aaacgataga ggctgaaaat gaaaaaagca cgcgtaattt
    13141 ataaccccac ctcgggaaaa gaattattga aaaaaaattt ggcggatatc ctacaggcat
    13201 tagaagaagc aggatttgaa gccagcgcct atgcaactac accagaacca gattcagcca
    13261 agaatgaagc atgtagagtc gcagaactag gttttgatct agtagtggca gctggtggcg
    13321 atggcacaat caacgaagtg gttaatggaa tcgctccgct tgaacaccgt cctaaaatgg
    13381 ccatcatccc agctggtaca acgaacgact atgctcgagc attaaaaatt cctagagata
    13441 atattaaagc agcagcagaa gtgataaaaa aacaccaaac cgtaaaaatg gacatcggcc
    13501 tttctgataa cagttacttc atcaatatcg ctgcaggtgg ctatttgaca gaattgactt
    13561 atgaagttcc atcagaacta aagagtattt ttggttatct tgcttatctt gcgaaaggag
    13621 cagagatgct ccctcgagta aagccaatca aaatgcgctt gaagtatgat gaaggggaat
    13681 atatcggaaa tgcttcgatg tttttcctag ggctgacgaa ctctgttgga ggattcgaac
    13741 agatcgcgcc agatgccaaa ttggatgatg ggaagttttc tttaatcatt gtgaagacag
    13801 caaatatctt tgagatcttg catattgcag cactcatgct gaatggcggt cgacatgtcg
    13861 atgatccaag aataatctat accaaaacaa gcagtttgtt cgttgaaaca gaagatgata
    13921 tcaatcgacc agtaatgatc aacttagatg gtgaatacgg cggggaagca ccgatgcatt
    13981 tccaaaatct tcatcaacac attgagttct ttgccaatac cgatcagatt cctgatgaag
    14041 caatcacagg tacagatgaa gaagaattag aggttgtcag caaagaattc gtaaaagaag
    14101 tcgaacgttt aacggatgaa gatatcgacg gtgacggaaa aatagcagac gaaacaccta
    14161 atcaaaagaa ttaatcaaaa gccggtgctg aacgtagtaa ggtcagtgcc ggttttagtt
    14221 gtgtaattag ctgatttata gtagagttca gtaggattga aaaataggct taaaggagca
    14281 aaatatgaca gaacagcaaa tagtaaagaa aaacgaacga tatacagtcg aaatcgtcga
    14341 cttgagttat gaaggaatgg gggtagcaaa aatcgatggt tatccattat ttatcgaaaa
    14401 tgcactccct ggagaagaag tagaaatact agtagtaaaa gcaggcaata agtttggata
    14461 tggaaaagtc gaaacattta ttacacaatc acctgatcgt cagcctgtag aaaacaatgc
    14521 attacttcgc ataggtatcg cacctttagc ccatttgaag tatgaacaac agcttgtctt
    14581 caaacaaaaa caagtagagc atgtaatggc gaagattgct aagatgccag aagttcctgt
    14641 acttccaacg gtcggaatgg cagaaccatt cggctaccgt aataaagcac agatccctgt
    14701 tcgccgtatc gatggacagt tagccaccgg tttttacaaa aagaatagcc atgatctagt
    14761 ccctatcgaa gatttttata tccaagatcc tgtcatcgat caagcaatca aagtagtcag
    14821 agatatcttg caacggttcc aagtacgagg atataatgaa gaaaaaaatg aaggacagct
    14881 tcgtcacatc attatccgtc gtggtcatta ttcccatgaa atgatggtcg ttttagttac
    14941 tcgtaaagaa aaattcttta aatccaagga aattgctgaa gctattcatg aagaattgcc
    15001 agaagtggtt tcggtcatcc aaaatatcaa tgaagaaaaa acaaatgtga ttttaggaga
    15061 caaagaaaaa gttttatatg gcagagacta tatcgaagat cagctattag gaaaaaccta
    15121 tcgaatctca gcaaagtcat tttatcaagt aaatactcaa caagcagagt atctgtacca
    15181 aacagccatc gagtttgccg agttagataa aaaagacatt gtgatcgacg cttattcagg
    15241 aataggtacg attggcttgt ctttagcaga cagagtggat aaggtgtatg ggatggaagt
    15301 tattccagaa gcaattgaag atgcacagtt caatgcttta aataataaaa tcgaaaatgt
    15361 tcattatgaa gtcggaaaag cagaaaaagt tatgaaaaaa tggcaagaaa aagggatcaa
    15421 accatctgtg atcgtagttg accctccacg aaaaggatta gatcatcatt ttattgaatc
    15481 agcaatcgaa atgtcaccag aacgaatcgt ctatatctca tgtaatccgg caacttttgc
    15541 aagagatgct aagttatttg cagaagcagg ttatgccgtg aagaaagttc agcctgtaga
    15601 tcttttcccg caaacccatc atatcgaatt agtcgcttca ttcgttaaag gagaataaaa
    15661 atggaccaaa aagaaaaaat gaaagaattg atcaaaaaga aacaaggtgg aggccgatcc
    15721 aaacaaatgg aaacacctaa gaatgatcgt aaaaacatga gaaaaggccc taaaatcttt
    15781 aataaataga cgtgctaaag aaagactaag aagtgttaac ttttgttatt gtttcttagt
    15841 cttttta
//
LOCUS       DAZUKZ010000057        15704 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000057 DAZUKZ010000000
VERSION     DAZUKZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.057"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..1489
                     /locus_tag="ACG8EF_001729"
     CDS             62..1489
                     /locus_tag="ACG8EF_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4947037.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1581..1946)
                     /locus_tag="ACG8EF_001730"
     CDS             complement(1581..1946)
                     /locus_tag="ACG8EF_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS4947038.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1943..2308)
                     /locus_tag="ACG8EF_001731"
     CDS             complement(1943..2308)
                     /locus_tag="ACG8EF_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS4947039.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2500..2748)
                     /locus_tag="ACG8EF_001732"
     CDS             complement(2500..2748)
                     /locus_tag="ACG8EF_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947040.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2747..2875
                     /locus_tag="ACG8EF_001733"
     CDS             2747..2875
                     /locus_tag="ACG8EF_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFS4947041.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2932..4626
                     /locus_tag="ACG8EF_001734"
     CDS             2932..4626
                     /locus_tag="ACG8EF_001734"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFS4947042.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4726..4899
                     /locus_tag="ACG8EF_001735"
     CDS             4726..4899
                     /locus_tag="ACG8EF_001735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947043.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5277..6410
                     /locus_tag="ACG8EF_001736"
     CDS             5277..6410
                     /locus_tag="ACG8EF_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFS4947044.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6441..6761
                     /locus_tag="ACG8EF_001737"
     CDS             6441..6761
                     /locus_tag="ACG8EF_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFS4947045.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6790..7254)
                     /locus_tag="ACG8EF_001738"
     CDS             complement(6790..7254)
                     /locus_tag="ACG8EF_001738"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4947046.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7386..8639)
                     /locus_tag="ACG8EF_001739"
     CDS             complement(7386..8639)
                     /locus_tag="ACG8EF_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4947047.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8668..8985)
                     /locus_tag="ACG8EF_001740"
     CDS             complement(8668..8985)
                     /locus_tag="ACG8EF_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4947048.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(9011..9331)
                     /locus_tag="ACG8EF_001741"
     CDS             complement(9011..9331)
                     /locus_tag="ACG8EF_001741"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4947049.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9795..11210)
                     /locus_tag="ACG8EF_001742"
     CDS             complement(9795..11210)
                     /locus_tag="ACG8EF_001742"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4947050.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11227..12543)
                     /locus_tag="ACG8EF_001743"
     CDS             complement(11227..12543)
                     /locus_tag="ACG8EF_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4947051.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12693..13391)
                     /locus_tag="ACG8EF_001744"
     CDS             complement(12693..13391)
                     /locus_tag="ACG8EF_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFS4947052.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13680..14669)
                     /locus_tag="ACG8EF_001745"
     CDS             complement(13680..14669)
                     /locus_tag="ACG8EF_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4947053.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14749..15582)
                     /gene="msrA"
                     /locus_tag="ACG8EF_001746"
     CDS             complement(14749..15582)
                     /gene="msrA"
                     /locus_tag="ACG8EF_001746"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4947054.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
BASE COUNT         4885 a         3184 c         2690 g         4945 t
ORIGIN      
        1 aagaaaacat gtcataatat ttcccatatt gtgcaaaaaa atacaaggcg gaggctctac
       61 tatgattgaa gaatatattg agaaaaatat tttaagacag ctgtttttat gtgggcaatt
      121 ctatgtaaat aaagaagtca atctagaaaa gctctccaat ctcctgcatg tttgcaaaac
      181 cactttactc aatgatatca ataacataaa aaaagaattt gaagaacaga tcgcttatac
      241 acatcgggaa aaggaccgtt acactttata tttttcagaa catatacctc gatgcaagat
      301 catgcagcaa ttgtctcaaa attcgttgtt tttaaaaact tgtctgcttt atttggaaga
      361 agatgaacct gattatcttc aattaactga atgtgaattc atctctgtca gcaaagctta
      421 ttcattaaaa aaacaagtat tagcttactt taatgactgc gggatcgaaa tcgaccgcta
      481 ctcccctcga ttcactgaaa tggaaagacg tctgctgctt cttaatgtgt cgtatcgttt
      541 aggcggtttt aattcttggg agctacctga atcgtttttt gaacgtgccg atcgtttcat
      601 tgaatccgta acggaaaata gtggcagatt ttatgataaa gaaaataaag aaatcttatc
      661 gattggtttt gctatcagtt tcctacgcca gcaagtgtgt gctgtgacaa tagattcaaa
      721 atttatcgaa gaaatcaaaa aacgtcctat ttacaattat gtcgaatcag catgggaaaa
      781 cactgatttc caaacctatt ataaaaaaga agaattcgcc tttattttga cactattcaa
      841 tctttgtaat tatggtttcc attcttatca gttgattgcg gaagactttc aacagctaca
      901 ccaagtgttt attgacaata ctcccgaaat aaaagaacta gttgctacct ttgaatctca
      961 cttcaatcaa gaattattcg gaaaccaacc gttcgaacgt gcgctgattc atctgatgcg
     1021 ttcggcttgg gataactatc aattattcat gccagaaaaa ttttatcttt taaatgagga
     1081 acagacaaat ttatataaag aagtccagac aattttttcc agctggtcca gccagttacc
     1141 ttatgatcta cggttgaatc ctaattgcat gcgggctttt gtgatcgaat tgagcggaat
     1201 tctccgtctg acaaaagaac atctgactat ctatatcgtc acaaattcgg atgttcatta
     1261 tctgatttat cgggaagcct tggaagctgt aactactttc gattttcaag ttgcaccaac
     1321 aatttattca agtatatctg atataaagaa gtatgcgcag cagtcctcaa accgggtatt
     1381 atgtgaacgt acattatata caccagatgc ggtccaatat gaaaatatta ttcctatttc
     1441 gatcaatacg atcgatcgag ctatcatttc agctgtgcaa aacaaataag gggctgtgac
     1501 aaaaatcatg tcacagcctc ttatttgttg aagatgccca tttctgtaac aatctcttga
     1561 tacacgtcag gctcacaacc tcacttattt tggacatcta ggggtccgat ctctttattt
     1621 aaaaaataag aaatgatggt tcggataact accaacgccg ctagttggaa aatatcctgc
     1681 aaggtaggtt tgataatcga ttcgatgatc ccagctgaaa caagaatttc caaacttaga
     1741 agtatatagc ctcccaacat ctttttaata gcattgttct cgcttactcg gtcgctttgc
     1801 acttttgtat ggacaaataa atatttcaaa aaatccttga ttgatagaca aacaccccaa
     1861 accaataaac agatagcaaa gatatcaagt cccagtacaa gtttttcgaa atatggaaga
     1921 agaaactgca tcaactctgt cgtcatccta cctctcctct tctttgtttt tctttgtctt
     1981 ctatttcctg attcaagaaa taagaaatga ctgttcgaat gataataata agtgcaagct
     2041 tcaaaatatc tgtccaagtc ggcttgataa ctgactcgat gatatcagca acgactagta
     2101 cttccaagct taacaaaata taacttgcta gcagttttct tgtatctgca ttacgagtat
     2161 tacgttctgc atagtttttc ttgacatgag accaatcaaa aagtttgaat agaacaagaa
     2221 cgatcccgac aattaagata ccgatagaac aaatatttaa aatagcaatg atccattcta
     2281 gaaattcaat taaatttgag cgaaacatat acgctaccta ctttctttta atttgttcct
     2341 attcttttat tgtaaaagaa agttaccttg ttcgtctatt taaaagttgt tctaatgata
     2401 acggttcgct taaatttttc tttttcactc tcccattttt ctccctccat ccataaatcg
     2461 tggaagtatc caaaacaacg tagcaagtta aaataaggct tagtccgtca tcagcttttc
     2521 aaagaagcac tgtctcggtg ctttttttgt acccaaattt ctaaaaatag aaccatacac
     2581 ctcctcaaat cttttcttct tacctaccct atttttattt acaggaaaat cagacacttc
     2641 ttcttttttg tcaaaatttc ctttttcttc tcgtcctatc atttggcatt ctaataaaaa
     2701 cgaactatcc ttggatatag aaagagaaaa caaggaggcg tttttcatga gtaaaaaatt
     2761 aatcaaagtc ggcatcggtt taggtttgct tgcccttggc gctgcatatc taggaaaaaa
     2821 gacaggacta ttcgaagatg acagtcattt atatgatgaa ttcgaatcaa tctagcatcc
     2881 tatttagttg aatcactacg gacatacttg gaagacattg gaggaatcac aatgagaaaa
     2941 agtaaagcaa taatgattgg tgccggtatt gccaatatgg cagcggctgt ttatttgatt
     3001 caagaaggca aatggaaagg ggatcagatc accttttatt cattggatga tcacggttca
     3061 aatgatggtg caccgaccga cactgtagtg gatgaatatt ggaataaaaa ccatccatta
     3121 gaaaaccaaa aaggctatat tgctcgaggc ggtcgcatgc ttaattaccg tacctatgta
     3181 gatttgatgg atctgttaag tcgaatacct tctgttactg agccagggat gactgctgaa
     3241 gaagatacga gagattttga tgcaaaacac cgaacctttg acaaagcacg tttgatggaa
     3301 ggtggcaaag gaatcattga tggcggaaaa ctgggattca ataataaaga ccgtattctt
     3361 ctgactaaac tgattgctat gcctgattca gaagaagaaa aactagacaa tatcactatc
     3421 gaagaatatt tcaaagatga tccacacttt tttgaaacaa acttctggtt tatgtgggaa
     3481 acaacatttg ctttcagaac acgtagttct gctcaagaat tgcgtcgcta tatgcatcaa
     3541 atgatttatg aattcactca aatcgaacat ctagtgggcg tcaatcgaac acgttacaat
     3601 caatacgaaa gtattatgct tcctttgatc gagtacttga aagaacaagg atgcaaaatc
     3661 attttgaacc gccgtgtcac tgactgggaa ttcaaagaaa caccgatgca agatgaaatc
     3721 actgtgactg gtttgaaaat taccaataca ctgactcaag aagaagaata cgttgtggtc
     3781 gatgaagata cagcagttat tttcactaat gggtccatta cagattcagc tactttagga
     3841 gattacgaaa caccagctgt agaaaatatg gattatggtg cagcttctag tctctggaaa
     3901 aaagccagtg aacgtttcta caacttggga aatcctgaca aattctttgc tgatcgagat
     3961 gcaagcgaat gggtcagctt taccctgacg acgaaaaacc atttgttgtt gaatgaaatc
     4021 acccgtatca cgactcaaga accaggaaat gccttgaatt catttatttc aaccacaccg
     4081 atcacgccgt taggccaaaa agatgtcaat atgtctattg tcgtgcatca tcaaccgcat
     4141 tttacgtctc agaaaccaaa tgaaacagtt atttggggtt atttccttta tccaagacga
     4201 cgtggcgaat ttgtggataa acagtatatt aaaatgaatg gaaaagaaat gctagaagaa
     4261 ctgattggac aactttcaaa agtagatcct ggaccagtga atattcgtga aaaagaaaca
     4321 gaaattttcg acagcgtaat aaataatatc cctgtctata tgccttatgc ttccgcgttg
     4381 ttcaataatc gtgccaaaag cgatcgacca aaagtcattc ctaaacattc gaccaatctg
     4441 gcctttacag gagaatttgt cgagcagcct tatcagatga tctttactga acaaagtgct
     4501 gtacgttctg gagaaatcgc tgctttccat tttgcgggta ttcctatgtc ccgtcttgtc
     4561 aaaacaccgc gttacgacaa agatattcct actctagctc gtgccgcaaa aaaaatgttt
     4621 gaataatcaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac aaccaggcta
     4681 aaacagcttc ttatgttttt gatttggttg gacttattac cgcagatgca agcttttgaa
     4741 caccgtttac caagaggtta tgaaaaagct gccctgcatc aagtaataag agcagccaag
     4801 ctaaaacagc ttcttatgtt tttgacttgg ttagacttat taccgcagat gcaagctttt
     4861 gaacaccgtt taccaagagg ttatgaaaaa ggcgtttagt ttcgagtatg aagaaaaatt
     4921 ttgtgaaaat ggttcctcac gtttttgcat aaatttgact taataccgag aaactgatcc
     4981 cttctgtcat aacctctttt tatttatgtc tgtttctttt tgaaaacaac tattttttca
     5041 acgtttactc tcttgccaat ccaacatcat ctaacggata caactatttc aatgattacc
     5101 attacactaa aaaatcggaa acacacctct ccgaacacaa atacataaga ctatagaatg
     5161 tttcatttaa gaaaaaaagc gtttcattat caaaaaggta agatctcctt taaaatgaaa
     5221 ttatctgtag tactaataat gcagacaatt ttatgaatct gaaaggagca tctccaatga
     5281 aagcagtcac ttggcaagga aaacagaaaa tggaaatcag aaacgtagat gatccaaaaa
     5341 tattagaacc aacagatgca atcatccgta tcacagcaac agccatttgc ggctccgatc
     5401 tgcatcttta ccatcatggt gaagcagttt tggaaaaagg gtatgttgta ggacacgaac
     5461 caatgggaat cgttgaagaa gtgggatctc aagttcaaac attgaaaaaa gggcaacgtg
     5521 tggttatccc ttttaatatt agttgtggtc attgtcattt ctgcctgaat cacatggaaa
     5581 gccaatgtga tgaatcaaat ccagatcagt tatatggcgg gcttttcgga tttggcaagt
     5641 tgaacggtaa tcattggggt ggtcaagcgg aatatctaag agttccttat gcagactcta
     5701 cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa agttttattt ctatcagata
     5761 ttttgcccac agcttattgg agcgttgaaa acgcaggagt aaaaaaaggc gatacagttg
     5821 tgattctagg ttctggtcca gtcggtctat tcgctcaaaa attcgctgtt atggctggag
     5881 cagagcgcgt aatcgcagta gatccagtac agcaccgtct agataaagcg gctaattaca
     5941 atggggttga aacttatttc ttagaagaca cggcacaagc tggaacagaa ttgtatgaac
     6001 tggctaaagg tggcgcagat gtcattatcg attgtgtagg aatggacgga ttagagcctg
     6061 tcaaagaaaa agcgaaaaat ttagtaagct tacaaagcgg cacaatctct ccattacaga
     6121 tggcatcgca agctgtcaaa aaatttggaa cagtccaagt aactggtgtt tatatgactc
     6181 cagcttcttc ttacccgctg caagaatttt ttatgcggga tatcgctgtc aagcatggac
     6241 aggcaccaat tatccatcta atgccaaaaa tttacgcaat gattgcagaa ggactattcg
     6301 atcctacaca aatcattact cattcgctgc cactatcaga agcagcacaa gcatatgaga
     6361 tctttgacaa aaaagaagat aaaaatataa aagttgtttt gaaaccttga attcagtaaa
     6421 aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa tacccatgag atcgaaatag
     6481 gaaaagaaga agatatcgaa atacgaggac gtcgcttccg cttctatttc caaaatcgtt
     6541 acacattgat ctcactggct gtcgatttat taaccgggat tttctatatt tttggaagcc
     6601 ttgcttcctt aactaaaatc cccgatcgat atggcaccta tttttatttg gctggtgcta
     6661 ttttcttgac gattcgtcct attctcagaa tcgtaaggaa catttttatt tacgacgaaa
     6721 aaaaagcaat gaagagaaca agcaaaaaaa aagaggcatg agaatttttc aaatctcatg
     6781 cctctttttt caccctatcg tatcactttg atacagacga gcggtcgtcc cactttcttc
     6841 cttgaccatt gtcaaaaatg tccactcgta tcgttcataa aacgtggtat gatctgtaat
     6901 taagtaaagt ttttcgtacc caagttgatg catttctttt ctgatttccg ctaatatccg
     6961 cttggctatc ccctgcttac gcttttccgg ttcaacaaac agggcacaaa gatttggtgt
     7021 cagatcaggg cggttatgga agtcgttttc aatgacgccc gctcctccga cgatggtttc
     7081 tttttcatct aagaccacat accattgagg aatactttcc ttattagcga ttccagtacg
     7141 aatactttcc tcatacacaa cagcaggaac tccccatttt gatgaaaacc aattcgcgct
     7201 ttgttttact aattctgggt gttctcttaa tttgatccag ttatatgtaa ccaatgttta
     7261 aacctccgac taaaataaaa cgtttacaaa aattatacaa gtctttatga aaattagcaa
     7321 tgacaacaat agtgatgtaa aaaaaaaaac ggaaaacaat cacttgttct ccgttttttg
     7381 attaatcaag catctaaagc ttcttttctt ctttcttgga tcgtagctac cttgacgaac
     7441 ggtagataga tcaaaataga aagaaccaaa ttgacagcgg ctagtacacc acctgcaata
     7501 ctttttgttg ccaatacacc gccaataatt ggtggagtta cccatggagg cataacggta
     7561 gctgctggca ctaaacctgt agaagtcgca aagtaagcaa cactaactaa gaccattggt
     7621 gtcaaaataa atggaatgaa catgatcggg ttcaacacga taggtaaccc aaacatcgtt
     7681 ggttcattaa tattgaagac acctggcgca atcgacaagt ttgttacaac catatatggt
     7741 ttgtttttac gtcctactaa ataaatagct aacagtaaac ctaaagttgc acctgttcct
     7801 cctaagttga caaagctgtc aaagaaaggt ttgttcacga taaacggtgc gctatgtcca
     7861 gctgcgattg cattaacgtt tgcttcaatg gctggcgcat tgattgtttg catcaaagga
     7921 tcgaccatat tcgctccgtg taaaccaaag aaccataaga atggtgtgat aaatgcaagc
     7981 agtaaggcag atggataaga gtttgcaagt cccataaatg gttcttggac cactgtatag
     8041 aaagaaccta cgatatcatc aacaccgaat gcagcaaaaa ttgcagcaac taaagcaaac
     8101 gcagagatag tgatcattgc tggtagtaat gaagcaaatg cttttgcaac tgccggtggc
     8161 acaccatcag gcattttgat gatcaatttt tcatttccgc ttaaacgtac aaagatttct
     8221 gttgaaataa tcccaacaat aatagcaata aacaaaccgt ttgagctcat acctaatgct
     8281 ccgcctaaag cgaagaaaga gcctaaagaa atcacacctg cggaaacacc gtcttttcgg
     8341 tagccgttag ctaaatgata agcaagcaag aaagcaatca agacagaaag aatggcaaat
     8401 gtaccgttcc aagcagcagc cccgaatcct ttccatgatt caccaccaaa aattgagttc
     8461 atcaattctt ggaacccggg aatcggtaag ttattgatca ttactgctaa tgctcctaag
     8521 atcattaacg gcatactcac cataaatgag tcccgaatcg caactaaatg ccgttgtgca
     8581 ccaatttttg atgctacagg tataaaatgt ttctccataa atgcaacaaa tttatccata
     8641 aatcttccct cttttcttta ctgtagatta cgcgttcaat cgacgataca gattaatgat
     8701 ctcttttgct aaatctgtaa aagctataga tgtcatcaga tgatcttgac tgtggaccgt
     8761 caaaagcgtg acttcaacgt gatttccttg tgcttcttgt gtcagcatct ctgtttgaga
     8821 gtgatgtgct tggactaaag attcctctgc atctttgatt ttttcatctg ctaaagtgaa
     8881 atcacctttt ttcgcagcag caatcgcttc cattgcatcg ctttttgcat ttccgccaaa
     8941 catgatcaga cccatgatgg cttctagatt tttttgatct tccatttatt gtatcctccg
     9001 ataattttta ttatttgttt attagttttt ctgcttggtc gagtactttt tcaccattca
     9061 tcatgccata gtcagccata ttgattacat ctaaaggaat gcctttaggt gctagctttt
     9121 cagcaaattg attttgcata aagcgtactt gcggtcctaa aagtaaaacg tctacatttt
     9181 tagattctag ttgattatcc gcatctgaag cagatacagc aaagatgtct gcttccattc
     9241 cttttgcttc tgctgccttt tgcatttttg tgactaggag acttgtactc attccagctg
     9301 aacatacaag catgattgtt ttcttttcca ttttaaacac gttcctttct atacttacac
     9361 ttgtatataa gcaagtagcg tgccaacttt aaaagcgctt taaaaagttg atatcatcac
     9421 atttaaggtc tataccaagt tgcatacaca ctaaaaaaga aagcgcacac taaacacaca
     9481 aattaatttt tattaccttt tttcttttaa cgtgcacaaa aaaagttgtg acacaaaact
     9541 tgtcacaact tttttcaaat aaacggtatt caaaagcttg catctgcggt aataagtcca
     9601 accaagttaa aaacataaga tgttgttttt actttgctat tcttattact tgatgcagga
     9661 cagctttttc acaacctctc aataaacggt gttcaatcag ctgaccctgg gtattagctc
     9721 aaaacgctgg attcacaacc tcttttattt caaataacca gtaaatacaa tgaacatcta
     9781 caaaaaactt ctatttattt atagatttcg tcaggcaaat ccaattctct cttatcaatc
     9841 aaagtctgat accacgaagc agattttttc aatgttctgc ttcgttcatc gttcaattcg
     9901 attcgaacta atccatatcg attcttaaat gcattcattg gtgacacaca gtcagtgaat
     9961 gcccataaca tatagccttc gcaatttgcg ccttcttctg ttgctttgat caagtaatgc
    10021 aaatgctgtc cgataaattc gatacggtaa tcatcttgca cgattccttt ctcatccata
    10081 tactgttctt catattctct tcccataccg ttttctgtta tcagccaagg gatattatga
    10141 taattttctt ttagatacat ggctatatca tacatgattt tcggatagat ttcccatccc
    10201 ctggaattat tcattttctt cccaggtaaa tcaaagactt cataatatcg ttcaggatgg
    10261 aaaggtgtct cttcattcca ttgataacga ggggcgcgaa cgcgtttagg ataatattga
    10321 ttgattccaa ggaaatctac tgtatgatgc tgaatcgttt ctaaatcctc tgttgcagga
    10381 tcgaaataaa tcgattgttt ttgacacaac tccaggaact cttctgtgta gtatcctttt
    10441 accatcggat caaaaaatac tcgattataa aacaaatcat acatataagc agctttttgg
    10501 tcttctggag aattggatcg agcatagacc atttcaggat ttaaaataca gccaatcctt
    10561 ccaccataat ttccgctgcg gtaaatctct actgctttag ccgttgctaa aactttatga
    10621 taattccaca tcatccactt ttttgtgttt tgttcatgcg gccagcgaac agcatccaaa
    10681 tagcaacgcg tttggggaac gattggttcg ttgaatgtaa accagttttt taccctatca
    10741 ccgtaacgat caaaggcaat ttgagcatac ttagcataaa aatcaactac ttttcttgaa
    10801 gaccagccat catatttttc caaaagaaaa gctgggactt cataatgttc caaacaaatc
    10861 atcggttcta cgtctgcttc gatcaaagag tcgatcattt cacttatatg ctgagcatat
    10921 tcttcatcta ctatacagtt ctcataatca gtaaaaaaac gagcccaatt gatagaagtc
    10981 cgataatgtg tcagatggat ggatttcatc agatccacat cctgctggta ttgttccata
    11041 aaattagttg ctacggctgg gccataccca ttatgccaaa caaaatgttc atttttatac
    11101 cagctatcta taaacgaatc ctgtccttct ttttttcctt tccaaccttc cgtttgccaa
    11161 gcagaagatg cagcaccaag aataaaattt tttggaatag taattttcat gatttcctcc
    11221 tgttttttag atcccatcat tttccacacg atttgctact tttacaaatg gcagatagat
    11281 caaaatagaa acaataatac aaacaagttg cgtaataact gcaccgatat ttcctgctgt
    11341 tgccagataa gcattgacga tcggcggcat tggccaaggg accattacaa ctgctttcgc
    11401 cgcaaatcct aaagcggttg caaaataacc aatcgttcca gtgataagag gcgttaagat
    11461 aaacggaata gctaaaattg gattcagcat gatcggcata ccaaaaataa ctggttcatt
    11521 gatgttgaag atcccaggag caagggataa cttggtgatt tgtttcatat cttcccgttt
    11581 tccaactaac aatactgcaa tcagtaaacc gatcgtgact ccagacccgc ccatactcat
    11641 atacatatcc caaaatggca tagtaataat gttcggtact tcttttccag cttcaaaagc
    11701 ttccgtattt acagcaattg ccgctaaaag caacggttcc cgtatcggct tcaccatttg
    11761 atttccatgg ataccaatca cccaaaagac ttgcgcaata aacatcaata gcaaaatacc
    11821 cggtaaccct tgtacgacac cttctaatgg gcgttgtacg acattgtaaa tgatatcata
    11881 aaggtacatt cctgtaagtt ctttgatgat aaatcccgca gccgcgatac ccgatacagt
    11941 cagaatcgtc gggaataaag cagaaaaact agcagaaaca ttcgttggca cggtatcagg
    12001 cattctcagc tgtagtttct tctgtttcct caaccagcaa taaagttcaa tagaaaggat
    12061 ggagaccaac attcccaaaa ataacccttt cgtatctgta tattgtttag ccaatacgtt
    12121 ttctactata acagtctgat tttcattcac taattcataa acagttggat taacagataa
    12181 ataagacatc actgcgatga taccagggaa tacaccatcc actttattca gcttggctaa
    12241 ttcgatccct atcagaaaaa cagcatagat ggtcaagaaa cttagtgtga catagctgat
    12301 ggcagatgcg ataggcttta attcttctaa aaaacgcaag aaacttatct gtgctaagcc
    12361 attttccgag tcaaagacca ttgctgaaaa caatgtgcca aaagcacccg taataattac
    12421 cggaatcaat gcagcaaatg cgttttttat caccatgata taccttaaat tattgattct
    12481 cgttgccacg ttccctaaaa cattggctaa tttatccata aaactcattc cagatcccct
    12541 cattcctgct tcttctcctt gacgtcatct ctttttgtct attagtcagc gcttacattt
    12601 cgatgaacag aaactattta ctcctctttt ttaccaaata taaaacaaaa attcatctga
    12661 tgtatttatc ataaaataca tcagatgaac tgtcaattag tttctttttc tcctattatt
    12721 ttctgtaatt ctctccgatt tattgccata tggacagtca ttgcatcaaa cgcagcaagt
    12781 gcattccgcc tttcgatggc ctgaacgatc tctgtatgaa tatcgatcgt ctcgattttc
    12841 agatgctgga tatcataatt tttattaaac aaagcaattg attcattaat gattgggata
    12901 atatgcagca aggctacgtt tccgctagct tctgcaatca tcgtatgaaa atcgatgtct
    12961 aattgaacat gttcttgtgt gccttctcga aaagaagttt caatcttttg cgttaatttt
    13021 ttcatttgga cgatttgatt cttcgttgca tattccgctg ccaaacctgc catcttaggc
    13081 tccaataaat atctcagttc aaataagtct cgtatcatcc gatcctgatc ttcaatcaaa
    13141 gaaaaaccga gtgggtcatt ggcaatccct cgcttatcac tgacaaatgt ccccgctcct
    13201 tgccgaactt caaggatatt cctggaagct agagcccgca cagcttctcg caccgtgctt
    13261 cgccccacct ctaaataatc ggacaattca tattcattcg gcagtttatc tcctacttgc
    13321 aactgcattt cctttaaata tctaaggatc ttgtcagtag ttacttctac tagggtttta
    13381 ttttcttcca aaccacatac ctccttttca tctgatgtat tttttcgtaa ttattataac
    13441 acgtttgaat catctgatga ttttttgtat acaaaattga ttaatccgtt catagacata
    13501 caaacgaaga agatatttac cataaaaaag actaggataa aattcactcc tagccttcga
    13561 tcatttttat tttgaacgac aaaaaatatg atgttcaata acagacgatc ttcattcgat
    13621 tcttgatgtc atcttttgtt ttttttaata tatggataaa aagtttgatt acatcgtttc
    13681 tattctagaa tttggtcgat tctcaattct tcttctggtg agaaatctaa attatccatc
    13741 attcgaacac tttcttgtaa ctgacttaac cgacttgctc cgaccagtac acttgtaaca
    13801 gatttttgtc gcaaattcca tgcaagggcc atttgcgcta atgattgtcc tcgtgatacg
    13861 gcgattgttt gcagtgcttt gattttttcc agagttgatc caacttgttc ttctgaaagg
    13921 aatggtattt ccttacgatg tgctctagaa tcttcaggaa taccatgtag ataacgattt
    13981 gtcagcaacc cttgagcaag cggactgaac gtgattgccc ctagcccttc ttcttctaat
    14041 actggagata gatcgtcttc tagtagtgca cgagaaaaca tattgtagcg catctgatga
    14101 ataatgaacg gtgcttcctt tctttttaaa atttcagtca ttttttttgt atcttctcca
    14161 ttatagttgg atataccaat atataatgct tttccttgtc ttacaagttg catcaaagct
    14221 tctgctgttt cttccaaagg tgtatcaggg tctgggcgat gatgatagaa gatgtcaaca
    14281 taatctagcc ccattctttg gagactttgg tcacaacttg aaatgatgga tttacgagag
    14341 ccccattccc catatggccc ttcccacata tgataacctg ctttgctaga aataatcagt
    14401 tcatcacggt aggcttgaaa atcttctcta aaaatacgcc caaagttttt ctctgcagct
    14461 ccagcagggc ctccataatt attcgcaagg tcgaaatggg taattcccat gtcgaatgcg
    14521 cctcttaaaa tctctctttg attatggata ggatcataat caccaaaatt ttgccataat
    14581 ccgagagata aaatcggtaa tttcaaacct gaatgaccgg atttacgata ttccattttt
    14641 tcataacgtg cactatctgc aatatacaat ttttctcctc ctcatcaaat ctttttaaaa
    14701 cgctttcttt tttctatttt tagtttacta cattttaaag aaaaggagtt aatcaaaatg
    14761 attttttcgt atattttttt gccatttgtt ttttactcgt tgatagatca gaaattgatt
    14821 acgtgcttgc tggatttttt tataacgttt cggttgtttt ttgtaaaatt gctgatgata
    14881 gttttctgct ggccaaaacg taccggctgg ttcgattttt gtgacgattg gttggtggaa
    14941 agttcctgaa ctgtctagtt tctgttttga ttgttccgct aattcttttt gtcgttcgac
    15001 ttgataaaaa atcactggac gatattgctt ccctctatct tgaaattgtc caaatgtatc
    15061 tgtgggatca gtaatttgcc aatagattgt cagcaactcg ctataactta tttctcttgt
    15121 gtcataaatg atctccacag cttccacatg acctgtgtaa ccaccactta cttgatcata
    15181 atttggtttt tcaacatgtc cgcccgtgta tcctgacaaa acagatagga tccctttttt
    15241 cgattcaaac ggctcgacca tacaccagaa acatccccct gcaaatactg cacgttcttg
    15301 atagtcagca tcttgcatgg catgcttgga aaagtcatat ttttgctcct tatcaaatcc
    15361 ttccgtaatc aataaataaa agtccattac atcaggcgtc agattcattc ggatagctaa
    15421 tggacgtagt gttgcttcta gttttgataa ctgttcttta acggatatat tttctcttat
    15481 gtcattcttt gtctgaatca atacttttcg ttcccaatct cgtgtattcg gattcaaaat
    15541 cagattataa agtgtttgaa tgaatatttg ttcctcatcc atagattctc ctcctttccg
    15601 aagaagacaa gctagtgact ttagctgtaa tttatcataa agtgaggaaa taagcgatta
    15661 cttcatcccc ctacagattt ttcgtataaa aaagagagaa tcct
//
LOCUS       DAZUKZ010000058        15656 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000058 DAZUKZ010000000
VERSION     DAZUKZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.058"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(537..1040)
                     /locus_tag="ACG8EF_001747"
     CDS             complement(537..1040)
                     /locus_tag="ACG8EF_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4947055.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1399..2424
                     /locus_tag="ACG8EF_001748"
     CDS             1399..2424
                     /locus_tag="ACG8EF_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4947056.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2747..3625
                     /locus_tag="ACG8EF_001749"
     CDS             2747..3625
                     /locus_tag="ACG8EF_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFS4947057.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4101..4217
                     /locus_tag="ACG8EF_001750"
     CDS             4101..4217
                     /locus_tag="ACG8EF_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS4947058.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5145..5483
                     /locus_tag="ACG8EF_001751"
     CDS             5145..5483
                     /locus_tag="ACG8EF_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947059.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5577..5807
                     /locus_tag="ACG8EF_001752"
     CDS             5577..5807
                     /locus_tag="ACG8EF_001752"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFS4947060.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6207..6578
                     /locus_tag="ACG8EF_001753"
     CDS             6207..6578
                     /locus_tag="ACG8EF_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFS4947061.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6852..7544)
                     /locus_tag="ACG8EF_001754"
     CDS             complement(6852..7544)
                     /locus_tag="ACG8EF_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4947062.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7547..8332)
                     /locus_tag="ACG8EF_001755"
     CDS             complement(7547..8332)
                     /locus_tag="ACG8EF_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4947063.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8569..9969
                     /gene="lacG"
                     /locus_tag="ACG8EF_001756"
     CDS             8569..9969
                     /gene="lacG"
                     /locus_tag="ACG8EF_001756"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFS4947064.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9994..10311
                     /locus_tag="ACG8EF_001757"
     CDS             9994..10311
                     /locus_tag="ACG8EF_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4947065.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10324..11997
                     /locus_tag="ACG8EF_001758"
     CDS             10324..11997
                     /locus_tag="ACG8EF_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFS4947066.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            12011..13642
                     /locus_tag="ACG8EF_001759"
     CDS             12011..13642
                     /locus_tag="ACG8EF_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFS4947067.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            14514..>15656
                     /locus_tag="ACG8EF_001760"
     CDS             14514..>15656
                     /locus_tag="ACG8EF_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFS4947068.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQ"
BASE COUNT         4974 a         2587 c         3202 g         4893 t
ORIGIN      
        1 ttggaataaa aaaataacat atctcatttt acgcttttgt tattttatac ttaaaaaata
       61 acgacacaga aaataccgtg tctcagattt ctctactact tctaaaaact actgtttttt
      121 tcctttgaaa tgccttcaaa aagtaggatc aggcagaata tactggcaga atcaaggaga
      181 agggagccta aataaggaga aaaaccatag aaaatataaa gtaattactt ataaaacaag
      241 ctttttatag aaaaataata tacctgagta gggtataaat taagacggaa ttcctttcta
      301 gattgagata ttgactagga agaatgttaa gattttaagc ctttaagcta aaaaaaaacc
      361 ctttttgtta aataagggga atatagcctg tcagtttcaa actgcaagtc gtcctgctgc
      421 aataatatag tagattagag atgaaaatag atttttttaa accaatgact gaataaaaag
      481 accgtcatag atacacattg gaatgtgtct attacggtct ttttttacga acgattttat
      541 tgaagacgga cagttcgggt caaaaataaa aaagctacga tcagaaataa gaataaaatg
      601 tatcccaaaa taatggctag acggtctaac ttatttgtcg atagatgttt gtttagaagt
      661 tgataggccc ataaacctat cagtcctcct gttgtattag ttatcaaatc agtgatgtcc
      721 gctacgccaa taccgaatag gaattgaatc aattctgcca agaagcttac aaggaaaata
      781 atccctaatt ttttagaaaa agaaagtctc ttgaaattga cattcaaaag caccccaaaa
      841 ggaaaaaaga caatcaagtt ataagtggtc tcacgtaata ccgtcgtatt ttcaaaagaa
      901 aaagtagaaa aagggacgag attgacattg ctgtaagaat aagtgagaaa ttgtggaaga
      961 ttaaatgata gcttgaataa aatcaaccat aatagaaaga ataaataaat ccctaataaa
     1021 aattttgata cataccgcat agacatctcc tccatttaaa aagtatacca tcgaaaaccg
     1081 ctgaaaaatc ataaaaaagt atgaagttta aggaccaacg tagttatcgt acgaaaatta
     1141 gaagcttttt tttgttggga atccggtttc tttaacaaag ctaagatagc gagttgcttg
     1201 gaaaagttgc tatctttttt ctgtcaaaac gactcggaat gttcgttttt tgttctaatt
     1261 tctccaagtt gtttactttt tgatactaag tttatgaaaa atcaagaaat agttgttata
     1321 ctaggtaatg ctgacaaacg ggattggcat ttcatcttgg ttcagctttt tttccaatta
     1381 ctaggaagga gggatagatt gttcaagact tattttcgtt tcaataaaat acaagataat
     1441 ccttttcgta tcatgaatct gatgctactt atctttattg tattatggtt aggttacata
     1501 aatcatgatt ttttgatttc aagtttttca tcattaggga tctttacttt tttctattat
     1561 caaaacatac cgatgaaata tttgctgaaa cgtatgtttc tgatcggttg cggactgttg
     1621 gttgctttcg ttttggggat cttatcgact tatgttttat ggcttgaacc atttgccgta
     1681 gctttggttg ctttttccag cagattcgtg ttgcgattat tccatatttc taaaccaggt
     1741 ggcttgtttt ttgcgatgct ttctgccatg ggaacgagca tgcagctgcc aatcgctcaa
     1801 ttaccgatcg tctcacttta tttttttatg ggtgtcgttt ttgccctgat tgcggcagtg
     1861 attacaaaat tactggattc acggcctgag cagacaattg aaaaagcaac tttaaaagaa
     1921 agatttcacg aagagccttt agtgatcatt gattctgttt tctacagcgc tgccttattt
     1981 ctttccgttt atgtcagtca cggcttgaat ctgcataatc cttattggtt gaccttgtca
     2041 tgtgcatcga ttttgctagc agaaaacttg gatgcgatga agcatcgaca agtccaatat
     2101 ctgattggat cgatgggtgg gttatgtgtg tcggcattct tgtcatttgt gccgtttacg
     2161 cagttgcaga ccatttttct gatcactttt ctatatggaa tcgcacaatt tctagttgct
     2221 agaaattatg cagttgctaa tatttttctt aatccgatgg cgttgatgct atctacactt
     2281 attcgtggag catacttgat ttctttgatc gaatatcgtt ttttagggat cgtcatagga
     2341 agtttcatcg gtttaggtgt tgcgtgggta atgacggttg gtttgcaaca ttatttaacg
     2401 gttgttcgtc aaaaattaga atagaaggac aaatatatgt gccaaaagag gttatgacaa
     2461 gatttttgtc acaacctcta attcaaggtg ttcaaaagcc agattctcag cattaagtca
     2521 aattcacaca aaaatgtgga aaggaacttt caaaaaaatt ttatatttat tctatcaaaa
     2581 aattttgttt atgatggtaa ctgaataaac tttcctgaga tggtcaatgc tttccttgaa
     2641 agtcttacag atattctttc ttcatagaaa gtttaagttt tatagttagt gtacaataaa
     2701 agtatgttta taccatatgg tgtccggata atcagtcatt taaacaatga ggttcaaaga
     2761 tatgaaaagg ggaaatttga tgaagaaaat aagcaatgaa atccaaattc tggcagaaac
     2821 tttaactctt cccaaagtcg ttatagagca aatgattgta caagcaaaaa acatccttga
     2881 tgaggtgttt gagcaatata gtcatgaact aatggatcct aataaagcaa aggcgactgc
     2941 aaagaaaata gacaaagtct atgaagagga accttttgca gcattagcta tttatctata
     3001 tgcagcttct caaagttgga aaaaaattta tcaaccattg aagattccaa gaactatcta
     3061 tttggcaaca atgaatgctt ttactagatt tattcatgaa catttccaag taacaggaag
     3121 ctatcgcttt gaccgtggtt tttggatttg gcattacact agtggcttga tctttcggat
     3181 tggtgaattg gagtacgaaa tgacatattt tccgaaaggg tacacggatc taagattaga
     3241 aggaaagcag tgcctttctc ttcatattcc ttccgatgca gatttatctc agaataagat
     3301 aagagatagt tacgaaagtg ccattcgttt ttttgaactt tattttcctg attaccatta
     3361 ccaagtgatg tacacagata cttggttact atcacccaat cttactaaat ggttgaaaaa
     3421 agagtcaaaa atctgtcttt ttgcagccga ttatcgatta ttatcagttg atgaacagga
     3481 cgatagtgga gtcccttgga tctttggacg agtagatgcc caaatacatg attatccaga
     3541 aagtacgagt ttacagcgac aagcaaaaga gcaattgctt gcaggcgagc acattggctc
     3601 tggcttaggt attcttactt cataagttta gtacttcgaa atagtttaaa agagcgtgag
     3661 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
     3721 gcaactcctt cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt
     3781 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttagt gttctatcga
     3841 acacacagta cgttcaatag gtaaactagc tataacaata gatattaaag ttaacggaca
     3901 agagggcctt ttttaggtag ctaaaacgta ataatgaaaa tttatttcca gagataggag
     3961 tcaatcagct agtctgatcg actgttttaa caagttgact ttcattttaa cttttgatac
     4021 catttaaaac acaaatcgta attgtttcga tttgtgtttt ttagcatgac tacaatcaaa
     4081 gaagaaagtg ggtcgtccat atggaaaaaa cagatatctc aagtgcatac cgccgcttga
     4141 aaagcccaaa tatcaaaaca cgaaaacgtg cgctgaagat catcaaagat gtgaaacgga
     4201 atagtgggaa aaggtaagaa gaaggaaact taattaaaaa aatagccgaa aaacgttttt
     4261 cggctatttt caataatcgg tttttgtgta aggtttccga aagaactgct gttgattttc
     4321 catttttaac atatgataaa ttggtagaat ctttcttttc aaattgatac cattccctaa
     4381 ttccagttat atagttaact acagaaccac aaagcacgta cgtttataaa agtattatct
     4441 gtagttattt ttcaattccc tttcaacctc ttattttgac cagtagtatt gatcacttta
     4501 ccttttaaca cctttgttcg tttttgaaac atttttagtc aagaatggaa acgtaactac
     4561 ataatcaaag gttttgcgaa atatttcatg aaatatttca tgtagaagcg agcaatacgg
     4621 ttgcaaataa ccattatcgt ttaataagca gttacgaaag ctcccaatat ctctaacgtt
     4681 cagctcactc catttctttt cttcaaaaaa atcagtgatt cgtatctctt tctatttgtc
     4741 gaaaaagtta actttaaaaa gtaacgccga aaaaatatgc aaaaaaataa aaaagcagtt
     4801 actattgcta gaataacatc aaggaataat aagttttggt tttcagataa tagtcgataa
     4861 gttgtgtatt ggaattttag atacagtaat aaagtatttt tcatgaaatt ttgtgagaat
     4921 cactattaca agtatcttta ataaaattaa tggttaagga ggaacttaaa gtagaatgta
     4981 aatgaatagt tgtagtcagt cgattgtgat actagactca aagttgcagg aataagtttt
     5041 tcaatttgtg attggaagat aaaaaatgaa aaagaagggc ttttttttat actttgttga
     5101 ttgtagtgtt tattttccct ctgtttagcc aagtaacatt ttctatgact caaccagttt
     5161 caagtgagga acatgataac ctttttcgtg ctacagaaga gtttaagaaa tatcaggaaa
     5221 atattgttcc ggagactcca ttagctagtg agccaattac tgatattgat ggaaaccaaa
     5281 taggatggta tatacagtac gaagttatgg tggtaaataa tgactcaata gtaaaaaata
     5341 cagtaggctt agagctaaca ccgattgaaa aggtgagttc attgttaacg tatatttata
     5401 ctgatgaaag tgacgagact gagatcattt taacggatta ctcaaaaatt gctactgata
     5461 aaaagtatat attgtggatg tagacactag tgaaatactt tatgatatag aaattgatga
     5521 aaatttacta gatgtagcac aagaaatcga tcatcaaaag caggaagcta ttcgatatgc
     5581 tggtagaaaa gggaaaagaa aatatttatt acgtgaatgt agcaaaagtt agagaagatg
     5641 aaaatgagtg gaaagaattc aagagtcgct actcaattaa ctcgactccg acatttacag
     5701 tttacagaga aggatcgata gaaaaaactg ttttttggac taaagaaagt ggaatgtctt
     5761 tagctgaagt agaagagttt ttggattatg ttagtatgca acagtaaggg agttcttatt
     5821 atgaaaaatt ttgacacata ttatgttatt gttttttcga tatctttgct gttaatatta
     5881 ctaactttaa tgcagtataa agagtatcca gagtatttta ttttaacttt tgtatattta
     5941 gtaagtagct acacacttct atataaattc agaagttctt ctagagtttc taaagtttgt
     6001 ttcttaatta gcttattgat tctataatat ttttgattta tataggaaat taaaaatatt
     6061 atagaatcaa cactcagaga acgggttggt ttttaataga tttaataagt actgataatg
     6121 aaatttctcc tggttgggaa aacgaagttg gttaccctgt agaagaattt tttcaagatt
     6181 actttgcgga gtatgccgat aggataatga gagattacgt aacagataaa actcaagaag
     6241 tcatcaatca atcccagtca ttaaatgaat tatctgataa ggcgaaagaa gaagcagttg
     6301 ccattatttc ttcagggatt agtgactata ttgatatgag tggtgaaatc aattatggcg
     6361 ataaagacaa aattgtacgc atgataaaag cttttatcaa aacttttgat gtgaaggcag
     6421 atcttaagtt taatgatact tacctagtag gaaaattaat taatatcctt gggacagaga
     6481 aagatacgga aacgtttatt tcctctttgt ctatgttgtt tacagataag catttttcaa
     6541 ctcagcttgg tggagaggaa ctagtggaaa tcttttaacc attacgccca gcattaataa
     6601 aagtatatca aaaaagtact cctgatgatt tttaagattg gtttgagaaa taatgtgaaa
     6661 acactatttt taatgttaat tatagataga gatgaacgcg tcatatggtt aaaagaatga
     6721 aaaagtcaaa gatacactgc tatatttcac taaggatata gcagtgtatc tttcatgtcg
     6781 tttcactggc agttttttat aaaaggaggt gtgcatttca gttgcttttc tttggtagtt
     6841 agttgaatcg tttaagaatt aacggatgtg gcagacaggt gctctgtcca aaaaccagta
     6901 aaatctttga tggttccatc tttatttagc tgtgcctgtt caaatactgt tttgttttta
     6961 ccgaatggat caattgcaat aattgttccg atccccgcac gtttggcagc aagtaatcct
     7021 gagtaagcat cttcgataac aagacaatca gccggagcta agctcagttt gtctgccgct
     7081 ttaagaaaaa tatccggttg cggtttccca gggaaagagc cgtcgtcata gacaatcttg
     7141 tcccagtcaa accagcgggc taaatcgaaa atatcaaaat aaaaagcaac attctctttg
     7201 acagtagccg tggcaatcgt taatggaatc tgttggtcag tcagctggtc aagagtcgct
     7261 tcaagccctt ttgtcaactg aagctcgttt tcattttgca agcaaagcat acgataatag
     7321 ctttcttttt cttctgacaa tacggcgatc tcagaggcgg tcaaatgctc tgaaatgaaa
     7381 tgtttcaaaa tctccgtatt cgtccgacca tgaatatttt ttaaaatatc ctcgtctgat
     7441 aaaccatctt ttgtatgatc atgtatcata tgcagccaag cagcttcatg cagatgagag
     7501 tccaaaaaca ttgttccgtt gaaatcaaat ataatccctt tcatagttac gctcctatag
     7561 tggtattttc ggttgaatca gcaatttgca gatcgacata ttgtgcatag tgctcttgaa
     7621 cctcccgcga gatctggcga tccgtaatga gcagatcgaa atcatgcaaa ttataaaaca
     7681 cataaaaatc atctcgattg aattttttat tatcaacgag tagatatttc ttacgggaat
     7741 tatttagagc gatctgctgt gcttctcctt cttcggtact gtaagtagag atcgcttcgt
     7801 tgtgaacgcc atttgaactg ataaatgctt ttgtaaactt tagtcttctt aaattgtcgt
     7861 tggtgatcgg tccgacaaat gcgccggtat ttttccggaa atcaccgcca atcagtagaa
     7921 tttctggtcc atctgcttga gcaagaatat caaaaactgg ataattattt gtaatgatcc
     7981 gaattttctt gttcagtaaa tgcgtcgcca atagttcgat tgttgtacct ggtcctaaga
     8041 aaatcgtttc tccctcgtga atcaaggagg ctgctgtggc agcaatttct tttttttctt
     8101 gaatctgcag agtggctttt tcagtgtgag aaagttcttg atctaaagtg taggaaagac
     8161 tttgagcccc tccatggaca cgcagcagct tgcccgattt ttctaattca tctaaatctc
     8221 tgcgaacggt catatcagaa acatctaatt gattgatgat ttcattaacg gtaaggattc
     8281 cttttttatt gaccatttct acgatagtaa gcaatctttc ttttttcaac attgtcttca
     8341 cctctaattt aaggataatc tatcatataa acggacaaca gtcaatctaa ttcgaacaaa
     8401 agtaaacatt tagggtggtt tttatgtttg ttacaataga ttttattgct gttttctggt
     8461 ttttatgatt atttgtgttt gtttttgttg acaaatgttt tggatgcgtt tacaatgcaa
     8521 tcagttagaa agagcgaaaa ctgattatag atttgaggag agatgtacat gcttaaatta
     8581 ccagaagatt ttatttttgg cggagccaca gcagcctatc aggttgaagg agccactaag
     8641 gaaggtggca aaggcgctgt tgcttgggat gactttttag aggaacaagg gcgattcagt
     8701 cccgatccgg caagtgattt ttatcatcaa tatgcaaagg acattgaatt gtgtgaacgt
     8761 tttggtgtaa atggtctaag attatccatt gcttggagcc gcatttttcc tgatggagct
     8821 gggaaaccaa atccagaagg aatcgctttt tatcatcgtg tgttcgaaga atgtaagaaa
     8881 agaaacgtaa cgccgtttgt gacgttgcat cattttgata cccctaaacg tttgtttgac
     8941 catggagatt ttttgaatcg agaaacaata gaagcatttg tttcctatgc cattttttgt
     9001 tttcatgaat tcaaagaagt caaggtgtgg agtaccttta atgaaatcta tccagttgca
     9061 accaatcaat atttgttagg tgttttccca ccaggaatca aatatgattt tacaaagatc
     9121 gtcgcctgtc ttcataatat gatggttgct catgcccgtg tagtgaacta tttcaaagaa
     9181 aacgaacttc cgggtgaaat tggtgtagtt cactcattag agacgaaata tgcggcaaca
     9241 gacgcgccgg aagataagca tgctgccttt ttagacgatg cattgtcgat ccgtttctta
     9301 ttagatgcta cctatttagg ctattattca actgaaacct tgactgctct tgatgagata
     9361 tgtgaagcaa atcaggccag ctatcatttt cctgaagaag atttcgttga attgaaaaaa
     9421 gcatccacgc gtaatgacta cttagggatc aatcactacc aatgccattt tgtcaaagct
     9481 tacgatggag aaaatgcaat ccatcataac ggtacgggtg aaaaagggac ctctgtttat
     9541 aaagtgaagg gaatcggaga acgtatttat aaagaaggaa ttcctcgaac tgattgggac
     9601 tggctgattt atccagaagg tctatatgac ttgttgctgc gaattaaaag cgattaccca
     9661 cattacaaca aaatctatat cactgagaac gggatggggt ataaagatca gtttgaagat
     9721 ggcattatta tggatcagcc gagaatcgac tatttgagag tctatttgga atccttaagc
     9781 aaggcgataa cagccggggt aaatgtgaag gggtattttt tgtggtcgct tatggatctt
     9841 ttctcatgga caaatgggta taacaaaagg tatggcttgt tttatgttga ttttgaaacc
     9901 cagaagcgtt acccaaaaga atcagcttac tggtacaagt tggtcagtga aaccaaaacg
     9961 attatttaat aagtgataat ggaggaagaa aagatgaata aagaggaatt acaaatgctt
    10021 ggttttgaga tcgttgcata ttcaggcgac gctcgcagta cattattgaa attattgaaa
    10081 gaagtaagac aaggaaactt tgaacatgtt gattcggcgt tgaaagaagc cgacgaaaat
    10141 ttaacccttg cacataattc gcaaacaaaa attttagcgg aagaagcatc tgggaaagag
    10201 atggagatgg gattcatttt tatccatggg caggatcatt taatgaccac tttattgctg
    10261 agagatctga ttcaagactt tattgtactg tacagacaga atcaaggata ggagaagaac
    10321 gctatggatg gaatcattaa acaaatcgaa aaagggaaac cattttttga aaaaatctca
    10381 cgtaatagtt atttaggagc cgttcgtgac ggatttttaa cagcgatgcc agctatttta
    10441 ttttcaagca ttttcatttt gattgctgcg gttcctgaga tttttggtta ccaatggcct
    10501 gaatctgtct ccacatggct atggaaagtc tataattatt cgatgggtgt tgtagggtta
    10561 ctcgtttcag caaccactgc tcgctgcttg gctgattcga tgaatcgtcg cttaccgaaa
    10621 aacaaagtaa tcaacaccac ctctgtgatg ctggcctctg tggtaagttt cctgttgtta
    10681 agcgtctctc cgattgaagg tggattttca acggaatata tggggacaaa gggactactg
    10741 gcttcctttg tcgctgcatt tttaacagtt aatttatata agttttgtgt gctgaaagat
    10801 attaccatta aaatgcctaa agaagttcct ggaacgattt cgcaaacctt ccgagatatt
    10861 tttcctttct ctttttcagt atttgcggcc gttttaattg atttaggcat tcgacaaatc
    10921 ttccaaatct catttgcaga agcaatcatt acggtgctgc agccgctatt tacagcagct
    10981 gacgggtact taggaatcgc tattatctgg ggagcgatgg cgctgttttg gtttgtcggt
    11041 gttcatggac cgtcgatcgt ggagccggca attgcggcaa ttatctatgc caatgtggaa
    11101 accaatctgc aattgttcaa ttccggtgaa caagcttcta aagtactgac ggtgggttta
    11161 gggaattttg taggaactat gggaggaact ggggcaacat tggttgttcc ttttatcttc
    11221 ctactctttg ctaagtctaa gcagttgaag gcagttggga aagcttcctt tattccagta
    11281 agtttcgctg tcaacgaacc attgctattt gcggcaccca tgattttgaa cccttatttt
    11341 tttattccat ttttgttaac acctgttgta aacgtttgcc tgttcaagtt ttttgtcgat
    11401 gtattgggca tgaacagctt tatgtacgtt ttgccttggg caactccagc accgatcggt
    11461 ttggttctag gaacgggcat gggtatctta gcttttgttc tagcagcggt tctaatcgtc
    11521 gttgacttcc tgatctatct accattttgc aaagcatacg atgctgtctt agtagagaaa
    11581 gaagcacaaa aagcagaggt gatcgcaacc gaggaaggtg cttctgccga tgtacgtgag
    11641 gaagtacctg tggcagcgag tcaagttatc agcgatgcaa ataaagaaat caatgtgttg
    11701 gtactttgtg ctggcgcagg gacaagtgcg atgttagcta atgctttaaa aaaaggtgca
    11761 tctgaatcga aagcaccgat cagtgcaatg gcgggtgctt atggttctca ttacgaaatg
    11821 ctgcaggatt ttgatatggt ggtcttagca ccgcaagtca attcttacta tgatgatatc
    11881 aaagaagaca ccgatcgctt aggaatcaag ctggttgcga caaaaggagc agaatatatc
    11941 ggattaacga gagatcctga aaaagctgtt cagtttattc tgaaccaatt tcaataaagg
    12001 aggagccata atgaaaatgg gaatttggtt gcgtaaagct gttttatttg cagctgtgtt
    12061 aggggctttg ggggctgttt caaaaactgt tgaagcatct gccagcgaat ttattcgcgg
    12121 agcagatatt tcgatcctgt cagatatgga aaaatctggt gcaaaatatt atgaagacga
    12181 tatttcaaaa gatgccttag agattttaaa aaataatggt gtcaactatg ttcgactgcg
    12241 tttgtggcag gatccttata atagtaatgg agaaagttat ggagcaggga caaatgatct
    12301 ggaaacgacg attgaccttg caaaacgagc aaaaaatctg ggacttaagg tgctgctgga
    12361 ttttcactac agtgattttt gggttgatcc tgggaaacag aatcttccta aagcatggca
    12421 aggtctgacc tttgaagaaa tgaatacggc attgtacgac tatacaaaga acgttctgtc
    12481 ggaaatgaag cagctggacg tttatcctga tatggttcag atcggcaatg aactgaatag
    12541 cggaatgctc tggccttatg gcaagtcttg gggagaaggc gggggagaat ttgatcgatt
    12601 ggctgctttt ttgaagtctg gtatccaagc tgttcgagac actcaaccta aaacaactcc
    12661 agtaatgctc cacttagctg atggtggcga tacaggagca tttacttggt ggtttgatga
    12721 aatcacgagt cgaggtgtgt catttgattt aattggagtg tcgtattatc cgtattggca
    12781 cggttcaatg aatgaacttc aaaccaatat gaatgcgatc agccaacggt atggtaaaga
    12841 agtgatcgta gttgaaacag cttatgggca tacaacagcg aatgccgata ctatgccaaa
    12901 tgcttttggt gaagccgaag cagctgcggg gggatatgaa ccaacaccga caggacaagc
    12961 agagtatttg ctggatcttg ccgatcggat tcaggcagta ccgaataatc gcggggcggg
    13021 cttcttctat tgggaacctc tttggtacaa cggaaatgtg agttgggcaa cacaagcagg
    13081 tatgaattat ctcagtgttc aatccactat gggaaacgaa tgggacaacc aagcgatgtt
    13141 tgattttgct ggaaacgcct tgccaagctt gcgtgcgttt aaacaagcag gggcgcaaat
    13201 aaatctagtt aaaaatgcaa gtttcgaaag cgatggacca acgaccagtc cttccaattg
    13261 gcaaacctgg tttcaacaag gaaccgctaa gggtacgatc aaaacggaag cgggcacttt
    13321 ttttggggac tacaaactaa ctttttggca ggatcaagca tacgaggcat cagcttatca
    13381 aaatgttagt ggcataagca aaggaactta cacattgagt gcctatgtaa tgtctggggg
    13441 agagtttgat actaatcagt tatatatcaa aaattatggt ggagcggaac taaatactgc
    13501 ggttgttaaa acaagtgaat ggacaaaaat tacaatcaaa gacattccaa ttacaacagg
    13561 tgtttgtgaa attggggttt atagcaaggc ttcaggaaat gcttggacga acgttgattt
    13621 tgtttcattg gtgaaacaat agactgcgga tcgtttcgat tcaagatcgg aggttccctt
    13681 cagccacatc gttagcgtag aaggcaggaa tcgaacctgc acctcgcctt tgccaaaaat
    13741 aaccagaaac gatttccagt catttttacc aaaacgagta aagcaccaga cttcatctac
    13801 actttgtatt atgctaaaga gagttcaacg atttcgtaag aagcaatgaa aaaattgcaa
    13861 aaagttgtgc agataacata aagtttgttt aagaaagggt aaccttagaa ttccgtcgtg
    13921 aatatgattt gagcacttag atggatagaa aaaagttctc ggaaaacagt tcgtttttcg
    13981 agaacttctt tttatgataa gaatgaaaca aattaactta tattatagtc tcttttggta
    14041 gggaaccatc ttatttttaa tgaaaatcaa gcattttagc tattgaagaa tgttgattta
    14101 tagggattcc ccctccatat tatgttggct ctttattccc actctgtttt caacgtaaag
    14161 tgtataaaac agaatgcgat actatcaagg gagttaaaac aatatacaga acagtaaaac
    14221 actataaata aaaataaaag tgcaataaaa gtgcaatttt tgcacctaat catactctgt
    14281 ttatatatga aaactagatt ctgaatccaa tatttggtta acgagaggcg ttcgaatttg
    14341 aacgtctttt atatattact ttcaaataaa tatgaggtag aatactatct attttctttt
    14401 taacccaatt gttataatgg agagtgtaaa atattttgtg taaatagaaa aaaggaagtc
    14461 ccttctgtag aatagagtta ccacaacaca ttcacagaag agaggacttc cctatgaacg
    14521 attttactac agaaattcta aagactctag cgaacaaagg cgatttgaat gaattattcc
    14581 gtgtccattt ggagaaagca gtcaatacgc ttctcaaaac ggagttaacg gctttcctag
    14641 attacgaaaa gtatgatcgc attggtttta acacgggtaa ttctcgtaac ggctcctatg
    14701 accgtacggt caagaccgag tatggggaac ttcatctcca gattccgcgc gaccgcaatg
    14761 gtgagttcaa gcaacagact gttcctgctt atagacggac gaatgacacg ttagaggaga
    14821 ccgtcattca cctcttccga aaaggtatta ccatgtcgga aatcgcagac ttgattgaga
    14881 aaatgtatgg gcattactac acgccccaaa ccatgtccaa tataacaaaa tcatttacag
    14941 aagaggtaac ggcgtttaat gggcgggagc ttcatgaccg ttatgctgct atttatatgg
    15001 acgcaacgta tattccgtta aagcggaaaa ccgtcgccaa ggaagctatt catatcgcag
    15061 ttggcattcg cccggacgga tcaaaggaag tattgagcta tgcgattgca ccgactgaat
    15121 ccatcacgat ttgggaggaa attttattgg accttcaaga gcgcggtttg aaaaatgtcc
    15181 tcctgttcat cacggatggc ttaaagggga tggtaggagc gattagtcgg ttctatccca
    15241 aagctcgttt tcaacattgt tgtgtacacg tttcccgtaa tatcagtcat aaagtgcgtg
    15301 tcgatgatcg taaggaagtc tgtgatgatt ttaaaatggt gtatcaagcc tcatctaaag
    15361 aggtggcgtt ggaagcacgt ggtacttttg cggaaaaatg gaaaaccagc tatccaaaag
    15421 tggttgaatc gattctttcg aacgatcact tgctcacttt ctatgatttc cccttggcca
    15481 tacgcaagag tatttattct acgaacttga ttgaatcctt taataaacaa atcaagaaat
    15541 acagccaccg caaggaacag ttccaaaatg aagagtcgat ggaacgtttc ttagtctcgt
    15601 cttttgatac ttacaaccaa aaattcctag gtcgcagtca taaaggtttc caacag
//
LOCUS       DAZUKZ010000059        15440 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000059 DAZUKZ010000000
VERSION     DAZUKZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.059"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="ACG8EF_001761"
     CDS             complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="ACG8EF_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFS4947069.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1195..3036)
                     /gene="dnaG"
                     /locus_tag="ACG8EF_001762"
     CDS             complement(1195..3036)
                     /gene="dnaG"
                     /locus_tag="ACG8EF_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFS4947070.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3218..5533)
                     /locus_tag="ACG8EF_001763"
     CDS             complement(3218..5533)
                     /locus_tag="ACG8EF_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4947071.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5718..6578)
                     /locus_tag="ACG8EF_001764"
     CDS             complement(5718..6578)
                     /locus_tag="ACG8EF_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFS4947072.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6637..7269)
                     /locus_tag="ACG8EF_001765"
                     /pseudo
     CDS             complement(6637..7269)
                     /locus_tag="ACG8EF_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            7436..8197
                     /locus_tag="ACG8EF_001766"
     CDS             7436..8197
                     /locus_tag="ACG8EF_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4947073.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8223..10814
                     /gene="mprF"
                     /locus_tag="ACG8EF_001767"
     CDS             8223..10814
                     /gene="mprF"
                     /locus_tag="ACG8EF_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS4947074.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="ACG8EF_001768"
     CDS             complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="ACG8EF_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS4947075.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12557..12913
                     /locus_tag="ACG8EF_001769"
     CDS             12557..12913
                     /locus_tag="ACG8EF_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4947076.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(13154..14158)
                     /locus_tag="ACG8EF_001770"
     CDS             complement(13154..14158)
                     /locus_tag="ACG8EF_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFS4947077.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            14324..15175
                     /locus_tag="ACG8EF_001771"
     CDS             14324..15175
                     /locus_tag="ACG8EF_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4947078.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            15192..15437
                     /locus_tag="ACG8EF_001772"
     CDS             15192..15437
                     /locus_tag="ACG8EF_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947079.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT         4482 a         3209 c         2731 g         5018 t
ORIGIN      
        1 tttttctttt aaaataaacc aaacagtttt actgatttct cttctggtca atagtccacg
       61 cttattctaa gaaatctttt aattgttttg aacgagaagg atggcgcaat tttcttaatg
      121 ctttagcctc gatctgacga atacgttccc gagtaacacc aaatactttc cctacttctt
      181 ccaatgttct cgtccggcca tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc
      241 gatcagtcaa tgtatctagt acatcttcca attgttcttt tagtaactca taagctgcgt
      301 gttctgctgg acttgttgct tcctggtctt ctatgaaatc acctaagtga gagtcatctt
      361 cttcaccgat tggcgtttca agggaaacag gttcttgcgc gattttcaag atttctcgga
      421 ctttttctgt tggaagatcc atttccgcac cgatttcttc tggcgtaggt tctcttccaa
      481 ggtcttgcag caattgacgt tggatacgta tcaatttatt aattgtttca accatatgaa
      541 ctggaatacg gatcgtacgt gcttggtcag cgattgctcg agtgatggct tgacgaatcc
      601 accatgtagc ataggttgaa aacttaaatc ctttgcggta gtcaaatttt tcaactgcct
      661 tcatcagtcc catgtttcct tcttggatca agtcaaggaa ctgcatgcct cgtcctacat
      721 aacgtttagc gatacttact actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt
      781 cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc ttcttccgca gttaaaaggg
      841 agacacggcc aatttctttc agatacatac gaaccgggtc attgattttt acaccggttg
      901 gcgcagaaag atcttctgtt tttgcttgtt cagctttttt ctcagcactc ttcaagctgt
      961 gaattgatgg atcaccattt tcatccacca cactgatacc tgcgtcttca atcttttgga
     1021 tcaatttgtc catttcatct gcattcaaag tgaatggtgt agcaagctga ttggataaat
     1081 catcatatac aacttgtcgt ttgggtttgt tttctttgat gaaagctgct actgctgctt
     1141 catacgtttt tttatctgtt ccatttgcca taaaagaagg ccccccttga gtagctattt
     1201 ggcttgtttt aactgtttcg ttaaattgat gatttcaatc gtcaattcaa gctcaagctg
     1261 tttgtttcca gttcgtctgg cttcttgctg catttgtttt tttaatgtta tttcatcagc
     1321 taagccagat ttgctgatca catctagcaa gtcttggatt tctcgtggcg aactttcttc
     1381 tgccaccggt aaatattcga tttccaccac caaccgcctt aaattttcat cttgcaaaaa
     1441 gtctaaaaac tctgcaaggt tgaattcata atgttgtgag acatatgcat cgaataaaaa
     1501 ataaacttct gcatatgcat catgaataaa ctgaaattct cgttctttta aaatattacg
     1561 gacagaaact tctttaaatg cacgatataa tagaatctgc tctgcctttt ctacttgcgt
     1621 caaaggtctc tttttccgtg cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg
     1681 aggttcttga cgattttgct gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt
     1741 ctctctgccg atctgaaact ctgttgacag ttgagataga tacatatctt gttcgattgg
     1801 tgaaacaact cggctaagtt caactagtaa ttcttcgagg tattcgatct tgtctttttc
     1861 attatctaaa tttttccctt gttttaaata acgtgattta aaagtaaaga ctgtttcgcg
     1921 cccatgagcc actaattctt ggaaagatgc ttctccatac ttacggagat attcatcagg
     1981 atcaagcttt tctggaacga ctacgacaga aagtgctaat cgactgtttt gccgaagcaa
     2041 ttcaatccct cgattggttg cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa
     2101 ttctttcgtc agcttttcca tttgttggat ctgttgagct gttagacttg tccccataga
     2161 agctaatcca gttttgatac cagcctgcca tgcagcaatc acatccataa acccttcgaa
     2221 caacaaaatt tctccgtttt tccggatatc gcttcttgct ttatcaagat taaataaaac
     2281 ttgtctttta ttgaacagtt ctgtttctgg actattcaag tatttgggct gatctttttc
     2341 ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt
     2401 acgaagtgga aacatcactc tttgataaaa acgatcagac atacggccat catcatgcgt
     2461 gacaaacaat ccagagcgtt ctaacaattc atttgaaatt gattcattct tgaatacttg
     2521 ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg aattcttcaa ttaattctaa
     2581 agacaaccca cgactttgca aatattctaa agctggctgt ccagcttttg tatgcaatag
     2641 catgtgatga tagatttccg ctgctttttc atgagcagca atcagtttcc ccgtagcaga
     2701 ttcaggattt tggacagcta tagtacgctg acgccattga tcttctaacg gtatctgttc
     2761 gaagtctgcc acttttacca ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa
     2821 agtaaacacg tttccgcctt taccacaacc aaagcaatga aaaatctgct tgtcttctgc
     2881 aacagaaaaa gacggtgtac gttcttcatg aaaaggacac agccccaagt aatttttacc
     2941 agactttttc aactggacat attgtccaat cacctcaacg atattcgttt gactgcgtac
     3001 tgtttcgata acctcttgag gaatccgttg agccattttt tcaccccctg ctctattgat
     3061 atcttttggg tttaacagaa gatatttcag gagaataacc ggtaaaaaat cgcactaact
     3121 gaaatcagtg cgattttaaa ggacacaatt atacatgttt gattgtatca tatttcattg
     3181 ctttttcaag tataacacat ttctatctca ttaagcatca atttgctact cttttcagta
     3241 actgattttg aatatcagcc ttccagtaca atactcgaag aacaatattt acgagcatga
     3301 atacaaagaa gtatcctacg ctctctccca accctaagtt tacttccaaa attccatgga
     3361 acagcattgc agctacatat atccctacga tcgtcgttac tgctaggaag atgcgcaatg
     3421 ggttgaaggg ataacaagct tttacaactg ccatgcaact gatcccaatc agcaagtaat
     3481 acatcaaagt agtagtttca gcatttcccc atgcgaatgt ttttccaagt agatacaccg
     3541 caatgatatt cactactaca agcaaggcat ttggtaaagc attaataagt gctgtaggca
     3601 aataacggta agtgattttt cttttatctt gttcaaaaga taaaaagaag gaaggatacc
     3661 cttcaatcgc taaatcaatc agcgtgattt ggatagggat aaacggaaat ccaattgctg
     3721 taacggcaca tataatcgat aaaataaatg aataaatcgt tttgataaaa aagatgcctg
     3781 agactttcgt cacattgttg accactcggc gtccttcaaa caggacttct ggcaatgtag
     3841 taaaatcaga gtccaacaat accagattcg caatctgacg tgttgctcca tcaccttctg
     3901 ccatagcaat gctgcaatct gcttctctta acgccaaaac atcattcaca ccgtcgcctg
     3961 tcattgcaac cgttctgccg ctttctttca attcatttac cagcaatttc ttttgctgcg
     4021 gactcactcg tccaaaaacc gtatagcgat gagcagcttc tcgcacttcc atttcttcag
     4081 taatttgaga aagatcgaca tacgcatcat aattcggtaa tcctgcacgt ctagcgatat
     4141 tggagacagt aaaaggattg tctccagaaa tcactttcag atccacacct tcctcatgaa
     4201 gataagctag tgtttcttga gcgttttttc ggattggatc atcgatttca aacagagcaa
     4261 ccggctcaac aaatgctggt ttttcttcgt tcaattgctg atttggagcg atccccaaca
     4321 taagtacgcg atagccattc gcttgggctg tttttagact gtccggaagc cgatcttctt
     4381 ccaccaaacg ttcaggcgct cctacgtaca cgactcccag ttctggaaat tccatcgcgc
     4441 cccatttgcg atcagaagaa aatgcgactt tgtttgtcac atcataccga ttagaaagct
     4501 caaaataatc tctcatagcc cgcatagtga tgttgttatc actgctttct gccagatacg
     4561 tccccataat ttctgggatg gctttctcgt acagcggatg tagtgcttct acccgctgga
     4621 tactcatttt cccttcagta atggtccctg ttttatccaa gcataatgta tcgacatggg
     4681 ccagcgtttc aattgaatac atgtcttgaa caaggattcg tttttttgcc agtttggtta
     4741 cccctgtcgt taatgcaata cttattagca ataccaaacc ctttggaagc atgcctaaca
     4801 aagctgctgc agatgcaata acagaaattt tgatagaagc atcgcggatg aacagtgctt
     4861 ccaaaaatag tatgatcccc aaaggaataa tcacgaaact agtgaatttg gatactttgc
     4921 gaatcgaatc tacaagttca gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc
     4981 ggacagcata attatctgca cctactcgga taacttcagc catgacagaa ccgcttgtca
     5041 aaaagcttcc agaaagcaat tcagctccag tttctttgac aatcaaatca gattcgcctg
     5101 tcaaaagagc ttcatttgct tctgcccgtc cagatataac ctgtaaatcg gctggtatct
     5161 gttctcctgc tccgattttt accaaatctc ctaataccag ctctttagta gaaatcgatt
     5221 gttctttacc gtcccggatc actagtactt ctgattggga aacaatcgat aatttccgta
     5281 caagatttct tgcatgtatt tcttgataaa ttccaataac gatattgacg acaataattg
     5341 ctaaatacgc catgttggaa tacgcaccta caaataacag acaaatccca ataccaaagt
     5401 tcagaaaatt gaataacgtc aatatattat cgctgaatat ttgttttgta ctttttgatg
     5461 catcttcctg gtagtcattt tgctggccag aagccatttt ctgaactacc tcttgctgtg
     5521 taagtccatt cattttcttt ttctccattt tccgccaact ttcaaaaata atcacgtgtt
     5581 taagtctagc gtgttccaag attaaaaaca acttgttttt ataaaattta tagaaaaaca
     5641 cacgaatgat tttacaaaaa agatgccaac ctttttgttt gaatcacccg tgtgctgttt
     5701 ttattatcaa tctatcttta cttatattgt tcctccaaac ggctcatcca ccaacctagt
     5761 gctgtaccgg ctataaaaag aacaggacaa gctagtaccc cagcaactcc caatcccgcg
     5821 taatcggtag gaatgatcat aatcgttgaa gcaaaaacta cacctaaaat gaaatggaat
     5881 agcttgctat atgcctttga gaaaatgtaa tccatgattt ttgacaatcc aagaacacaa
     5941 agaatcccac caattccgat cggcacaatc acttcaagtc gtacttcttt aaatccgtca
     6001 gccatcgctt tatacaatcc catatacacc aaaaaattag aagggctcaa tcctgggacg
     6061 atcataccca aaccaatcaa tccgccggca atcagccatg ttccaaaatt ctgatcgact
     6121 tgcccgtcaa ataattgagc gccgtataat aaaaacaaat aagccagaac aaaagtaaca
     6181 accatgatga tcacatcaga agtatctctt ccttttttcc ctgcttcttt ccataacgca
     6241 ggaacagttc cagcgataca tccgacaaaa aaccataata tcgttgtttc atacgcacct
     6301 aataaaaaac tgactaaaaa agaaagtaga aagatgcctg tcactccgcc tagaccaaca
     6361 ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa ggtgggctaa aaaagaaatc
     6421 agccgctcat acatgccaaa gacagctgct aatgcaccac cgctgactcc tggcaagata
     6481 aatcctgaac cgataaacat tcccttcaca aaccgtaaca tccagtcttt ggttgtgtac
     6541 cccacttctt gattttccat tttttgcctt ttttccacta tttgcgcact ccttctttca
     6601 tttaatttta ttcatgattg aacatttcct gctattttac ttaaagactt tttcataggt
     6661 ctctattatt ttttcattca ttttgacaat atcaggataa aacatgttac ctccattatg
     6721 atagagatag ccgccattgt aaagcaagga ctggatccgc caatatcggt attgtttccc
     6781 ttcctcattt cctaaaagtg ggctcagcac atctttagaa tacgcttctg ctaattctac
     6841 tgtgttttct ccgccatgat tttttacata ggcaatataa tctttcccaa aattatatgc
     6901 ttggacagct gtccataaat cgcatccttg tttctttgct tctactatca tttccgccaa
     6961 atatttgaca ccttgatcaa tactgtcagc tggattatca atgatctcag cttctccatg
     7021 cagactttcc gagctctgca tgagatctgt cccttttcct tttgattcgg tcaatattat
     7081 cgccttgatc aaattctcgt agttttccat atgataattt tttgcagctg ttgttatttc
     7141 tatttgatag ctttccactc gttttaccga acgataagtt cttcctcata aaaacaacac
     7201 cgcaccaata accgtcagaa tgatcatgta aaaaagaata cggataatag ataatcgttt
     7261 tcgcttcatt tcatcaccta gttattttga catctttttt tatcttacta tttctttatc
     7321 ttcttggcta tttcttcctt gatttctgtg tacgccttaa gaaaaaatga attttcctgt
     7381 attttttgac atcttacgaa aatgatcgtt gtttttcagt aaaaacaatt gcccaatgac
     7441 tgatataatc gttatacttt acacagtagg tgattatgtg aaaaaagaaa agaaatatgc
     7501 aaaaatgcca gatgggagcg aaatttacta tgaaaagagc ggtcaaggct tccctctctt
     7561 tttactgcat ggcaatgacg gcagcggccg ctttttttca gaacaggtcc ctgtactcga
     7621 acgctactat accgtgtatc tagtagatag ccgaggacat ggtcgttcga caaacgaagc
     7681 atctatgttg aattttcagt taatggccga ggatctgaat acgatcatgt tgctggaaaa
     7741 aatcgatcag gcagattttc ttggatttag tgatggagca aatctagcac tggtatttgc
     7801 cagcagcttc cctaaaaaag ttcatcgatt gattttaaat tctggaaaca cgcttgttaa
     7861 aggcgttcgt ttttctgcca gagtcatcag taatattcat tatgcatggg tatggttact
     7921 ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt atcaaacttt tgctgcatga
     7981 catcgggctt acagaaaatg atttaaaaaa aatcaactcc cccactttga tcatcgtggg
     8041 aaaaaaagat gtaatcaagc taaaacattc tttgtatata gctaaaacta taccaaaagc
     8101 ttcctttgtg cttgtgaaag aacaaggaca tgaattagct agaaaagatc ctgaacgctt
     8161 caatcgagaa gttcttcaat ttctttctga aacataacta ttaggaatag aaggtgattt
     8221 atttgttaaa aaataccata caatggctga aaaaccacct aggactgttc aaaacaatct
     8281 ttttgatttc tgtcattgtc attattgtcg gtgaattgat gtccatcggt aaaactctat
     8341 ctatccagca attaggagaa acgtttgcca ctatacctgt atggaaaagc ggactaatgc
     8401 tattgattgg tcttatctct gtgttaccta tggttggtta tgatattatt ttgaatcgga
     8461 tgcttgaaca aaagcagaat cctcgttacc tgtttgagac aagctggctg atcaatacga
     8521 tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg gcttcgctct gaactatatg
     8581 gacaaaagaa agagagcaga aacgtgatcc aagcattatc gaaagttttc ttgtttttga
     8641 tggctggact ttctatttac agtctgcttt cttttttact tgtagtcttc acgccagttg
     8701 ctcctttttt gaagcaatat tggatatggt tgatcggcgg cggtctatat tttcctgtcg
     8761 tctttttatt tactactttc aaaaagaaag gattccttgg tggtacctca cttaaaactc
     8821 aaggacagct attgctagtt tcattgttgg aatggaccgg tgtcctgttt agtttccttt
     8881 ctgttggtta tttaatggaa atccggattg atctgtggca aacaattcca ttattcattg
     8941 ctgcttcagt gataggaatc gtgtctatga ttccaggaga aattgggagt ttcgatgtaa
     9001 tgatgattat cgggttgtcc gctattggtg tgccaagaga aatagtcgtt gtgtggatct
     9061 tgctctatcg cctgttctac tacatcattc cgtttttgat tggaatcgtc tttttcttca
     9121 aaaatatcgg ttccactttt gatcagcgtt attctggtat ccctaaacaa ttagcaacag
     9181 aaattgcgca taaaatcgtg gttgtcttat tatacttttc tggaatcatg ctagtacttt
     9241 ctgccactat cccgcaagct tttacagagt ttcgctggct tcatagtttg aacccattga
     9301 aatttcattt tattatccaa tttcctagta tactattggg atttctttta atcattatgg
     9361 gcagagggat cgcagcacgt gtgaaacgag catatttgcc tacaatcttc ttgattgttc
     9421 tagctttact ttatgtttta ttaagtgatt ttagtttcac accggtggtt ttcttgtcta
     9481 ttctgcttct gatcattctt gcttctaaaa acgaattatt tcgggaacaa ttgatctatt
     9541 catgggagtg gcgtacgatc gatggaatca ttattggtgc actgtccttg ctttacatcg
     9601 ttattggggt atacaatctg ccggatttcc ctcatcggcg tcaccatttt atttcctttt
     9661 tcttatttcc ttctgaaaag atttggtttt cgggattgtt ggccatcatc gctgttagtt
     9721 tcatgatcgt tctatttgtt catttccttc aaggagaaaa gaaacagatt ggcgaagcct
     9781 ttaatgaaga aaaggccctg aagatcctta caacttatgg aggaaactcc gatagtcaat
     9841 tgatcttctt aaaagataag cggatgtttg cctatgaaaa agacggtgaa ccaactgttc
     9901 ttctccaatt cgcttgcttc aataataaat gtattgtaat gggtgatcct tctggtaaga
     9961 aagaagattt tccagaagca atcgaagctt ttatcgaaga aacagatcga ctatgctatt
    10021 taccagtttt ctacgaaaca agcgaagaga ttgttatgat tttgcatgaa ttcggctacg
    10081 atttcattaa gatgggggaa gaagcttatg tagacctgaa tagttttaca acttcaggga
    10141 aaaaaatgaa gggcactcgt gctgtcttga atcggatcga acgagaagga tttacatttg
    10201 atgtcttaca accaccattt tctactgagc aaatgagtac tttcaaaaat atctctgata
    10261 attggttggg ttcacgaaag gaaaaagggt tttctttagg ctttttctct gaagattatc
    10321 tacaaagagc gcctattgca gttgtcaaaa atacccaaga agaaattgtc gcttttgcaa
    10381 cgatcatgcc tacttacacg aacaataaag taggaacgat tgatctgatg cgatatgatt
    10441 ctaaaaaagc accttctggc agtatggatt tcttgttctt gcatttgttt gcctatatga
    10501 aagaagaaca tatccaatgg ttcaatttag ggatggcccc tctttcaaat gtcggaactt
    10561 ccagaaagag ttttttacag gaaaggattg catacttggt ttacgagttt ggttctcatt
    10621 tttattcttt ccatggatta agagaatata aaagcaagta cgctacaaac tgggtctcac
    10681 gttatactct atattctaga gatagttgga ttgcatatgt tatgattgct ttgctgatta
    10741 ttgataatgc gccagttgag cagacacaag gtagaataag tggtttgaaa aaatggatac
    10801 gaagaaagta ttaaaaactg cataccgata gatgaacact ctcatgatga ataaaagaga
    10861 ctgaagtaaa acgaaagttc atgcttctac aatctcttct tatacaattg aagaacaaag
    10921 agcggtccaa acagaaatcc tgtttggacc gctcttttat tttaaacatt tttcattacc
    10981 aaaatattcg agtcgcgttt cttatgcttc gatctctgta acgatacctg aacctactgt
    11041 ccgtccgcct tcacgaattg agaatgtcgt tccgttttca acagcgattg gatggatcaa
    11101 gtcaacatca atcgtcacgt tgtcaccagg catgaccatt tctgtatctt ctggcaatgt
    11161 gattgttcct gtaacatcag ttgtgcggaa gtagaactgt ggacgatagt tgttgaagaa
    11221 tggagtatga cgtccacctt cttcttttgt caacacgtac acttctgctt tgaattttgt
    11281 atgtggagta atggaacctg gtttagcaag tacttgtcca cgttcgatat cgtcacgttg
    11341 gatcccccgt aacaacacgc ctacgttatc cccagcttcc ccgtaatcta acgttttacg
    11401 gaacatttct acacctgtta caactgcttt ttgtgtttca ggtttgatcc ctacgatctc
    11461 tacctcatca ccgacacgaa cagcaccacg atcaatacga cctgaggcaa cagttcctcg
    11521 acctgtaatt gagaagacgt cttccactgg caatagtaat ggtttatctg tatcacgttc
    11581 tggtgttggg atatattcat ctactgtatc catcaattcc ataatagcag cttcagcatc
    11641 tggatcgcct tgcaatgctt ttaatgcgga acctttgatc acaggagtgt catcgcctgg
    11701 gaaaccatat tcgctcaata actcgcgaac ttccatttct accaaatcga tcaattcttc
    11761 atcatcgact aaatcaactt tgttcaagaa aacaatcagg tattttacac caacttggcg
    11821 tgacaataaa atgtgttcac gtgtttgagg catcggaccg tcagttgcag aaacaactaa
    11881 gattgcacca tccatttgtg ctgcccctgt aatcatgttt ttcacatagt ccgcgtgtcc
    11941 tggtgcatca atatgcgcgt agtggcgttt ttctgtttca tattctacgt gtgcggtatt
    12001 gatcgtaatc ccacgttcac gttcttctgg agctgcatca atactagcat agtcttgagg
    12061 atttgctagg ccttttttgc ctaatactgt agtgatcgca gcagttaaag tagttttccc
    12121 atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt ttacttctgt cataatgttc
    12181 ttttgccata atttacaaac ctcctaaata atatagatca ctgaaaaatt tcagctctca
    12241 cctttttatt atagtctttt tttgactaaa tcaaaaagaa tacgtccgac ttactttttg
    12301 taagattcga actaagctta ttatacgtca atattggtca aagtcaacta agagcactcg
    12361 tctgtcttat acgcgaaaaa agtacctttt ataaataaaa aaatgtgttt tttgagaaaa
    12421 acttcaagat agtccttaac tttcttttga agcaataaga acaaaatgag gaaaagttta
    12481 aaattcagct gttgccaaca actttcaata gacgaaatct tctatttcgt taataataag
    12541 gaaagaggtg aagtacatga aaaaattaat tggcgtactt gtcgttttag gcatatttct
    12601 cggcggaagt tttgcagctt atcattattt ttacggcggc gaagcttatt atacaaaaat
    12661 cacgacgaat ggcgaaatgt cagctaccca agcagataac ggagagaaat tcacgactta
    12721 tacctacaag caagcggctt ataataaaga tggtgaaaaa aaacaggtga cgcttcgcga
    12781 agaaagagaa catccattaa agatgaatgc gtatcttaaa ctcaaagtca atcctcgtaa
    12841 aggtgtatta agctgggaag aaataaaggc ttctgaagtg cctaaaaatg cggcagaaaa
    12901 aatcgataat tagtaaatgg tcttaaaatt ttattagata aaaagaggct gggacagaag
    12961 cgtttaactc caagaaataa gaaggagttg cttctgcttc cgccgtttat acgtgtttag
    13021 agcgtgagac aaaagtgttc tttacttttg tctcacgctc tttttttgtt atattcgtct
    13081 tttccgatcc ttctcttgac acaattcatt taactgatca ctggtacttg aacacctaat
    13141 attctagtca tttttataaa cgattcaata cttcatccac atttttgatc atcacggata
    13201 tcgtaccatc cggattattc acaaattcaa tcaaatctgg gttgcgatat acatctactg
    13261 gtacgatcag ttcgatcccg tttgatagct tgaatttttg ttttccaaat tttttctcag
    13321 aaatttcttg tacttctttt acaggaggtg cttctgggac aaatccttgc tcttgtactt
    13381 cttctttaaa agccattttt gccgtgatgt tttccttgaa gactttttcc gcaatcatct
    13441 catgatccaa ccgacctttt tcttctatcg tatcatagac agcttctttc aaatcagcca
    13501 tgatatcaaa ttcttctgtc tcaaattttt ttccaagatg cttggctact ttttggatca
    13561 cttttacatt ttcttctaaa gaaggtgcag gaacactttc gatcactctt ccagaaaaat
    13621 aaagctcttt tttgccagaa aactcatatt ttttttcaat taattcgtag gtccaatcag
    13681 tcaaattcac agcgaatgct tcatctgctt tttgtgtttt cgcccctaga attgctcgat
    13741 tcaaaatcaa gcggttgtcg atccctgctt cgtctgcttc aacaaagtga gtgtaagcct
    13801 cacgataatt gactttcaat aaagataagt acatcactgt atctaattca tacaagacaa
    13861 caaatacatc tgcactaggt gcttcttcac tttcttggta gatatcgaac caccgctgaa
    13921 cgattgcttc acttgcttcc acaaaatttt cttgagcgat tttaagtgat tcaacgatca
    13981 tgctttcttc tgctagttgt ccagtttttg tttgagcaga agagattttt tggatcttct
    14041 tttgaagaaa atcacgaata tattcttttg tcagatccag ctctttttgt gaaaagactg
    14101 gagaaccagt ttctctgtca ataatatgca gaattgcttt ttttaaatag atatccattt
    14161 tctcattccc ttctgtctta gtttacggaa aaaaaatcaa aaataccaga atcaaaatca
    14221 ttttctttta aacaaaaatt tcaagagtag aaacaaaatg gattcttttt ttcgaaaaat
    14281 gcgctacact tatagttgaa cgaataaaaa aggagctgaa agtatgaatt cattaacaag
    14341 cacttatcgt ctatcaaatg gttacgaaat cccggtagta ggattcggca catggcaaac
    14401 acctgatggg gatgtcgctg tttcctctgt taaagaagcg ctggctgcag gttatcgcca
    14461 tatcgatact gcccaaggat acaagaatga agaaagcgtc ggccaagcga ttaaggaaag
    14521 tggaattcct agagaagaga tattcttgac aacaaaatta tggaatgcaa accacagcta
    14581 tgaacttgtg atgtcttctt ttgaagaatc attgaagaag ctccaaacag attatttaga
    14641 tctgtttttg atccactggc caaaccctgt tgcttttcgt gataattggg aacaagcaaa
    14701 tgcagataca tggcgagcat ttgaagaact gtatgaagca ggtaaaatca aagcaatcgg
    14761 tgtgagcaat ttcttacccc accatctaga tacactagca aaaacagcaa aaattatgcc
    14821 gatggtcaat caagttttcc ttgcaccagg cgaattacag acaactgtag ttgagtatgc
    14881 aaaaaaacat gatatgatcc tcgaagcata tagtccatta ggcactggta aaatcttcga
    14941 tgtgcctgaa atgaaacaaa tcgctgaagc acatgacaag acgattgcac aagttgcttt
    15001 gcgttggtcg ctgcaacacg aattccttcc tcttcctaag tcagtgacac caagcagaat
    15061 caaagaaaat actgaattat ttgattttga attaacagaa gaagaaatga aacaaatcga
    15121 tcagttagat ggtgtagtcg gcaaagcaaa agacccagat actacccaat tttaagtcga
    15181 aaggtgattt tatggatcca atcgaacgct tgaattctct ctcggaagaa gttacccaga
    15241 cttttcattc cgattttgtc tttttgatcg atgcagagaa aatccaacat tttcctgcaa
    15301 gaaactggac acatgatcaa atcatcgaag aactgaaaaa acggttcgat cattcgctaa
    15361 tggtcaaacc ctggcacgaa catgaagtca tttattcacc tgaactacct gtttttgcct
    15421 tgataccgaa aaaatagaaa
//
LOCUS       DAZUKZ010000060        15072 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000060 DAZUKZ010000000
VERSION     DAZUKZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.061"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..170
                     /locus_tag="ACG8EF_001773"
     CDS             <1..170
                     /locus_tag="ACG8EF_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4947080.1"
                     /translation="YQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRAR
                     IYLQQGLIFEN"
     gene            complement(553..1281)
                     /locus_tag="ACG8EF_001774"
     CDS             complement(553..1281)
                     /locus_tag="ACG8EF_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4947081.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1259..2833)
                     /locus_tag="ACG8EF_001775"
     CDS             complement(1259..2833)
                     /locus_tag="ACG8EF_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS4947082.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            3062..4000
                     /locus_tag="ACG8EF_001776"
     CDS             3062..4000
                     /locus_tag="ACG8EF_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4947083.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            4012..4923
                     /locus_tag="ACG8EF_001777"
     CDS             4012..4923
                     /locus_tag="ACG8EF_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4947084.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4947..6419
                     /locus_tag="ACG8EF_001778"
     CDS             4947..6419
                     /locus_tag="ACG8EF_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4947085.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6470..7081
                     /locus_tag="ACG8EF_001779"
     CDS             6470..7081
                     /locus_tag="ACG8EF_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4947086.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            7111..9024
                     /locus_tag="ACG8EF_001780"
     CDS             7111..9024
                     /locus_tag="ACG8EF_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFS4947087.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            9152..12133
                     /locus_tag="ACG8EF_001781"
     CDS             9152..12133
                     /locus_tag="ACG8EF_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFS4947088.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            12130..13791
                     /locus_tag="ACG8EF_001782"
     CDS             12130..13791
                     /locus_tag="ACG8EF_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFS4947089.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13831..14763
                     /locus_tag="ACG8EF_001783"
     CDS             13831..14763
                     /locus_tag="ACG8EF_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFS4947090.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4894 a         2389 c         3030 g         4759 t
ORIGIN      
        1 gctaccaatc gggtatttca aatgccttta aggtgtccca ctcaaacggc gtcactgagg
       61 ggctgaataa caagatcaag ctgattaaac gaattgcgtt tggttatcgc aacttttata
      121 actttcgggc acggatctat ttacaacaag gtctgatctt tgaaaactaa aaaaatatgc
      181 ggcgcacaga acgcgccgca tatcggacta agttttattc aaatttgtct tcaccaacac
      241 gatttgacga agagcctatt taaataacta catccattta ttctatcttc taaaatagcc
      301 tttttcattt cagttaaata gttcagcgcc atttttgcac tcgacttgca tagtagtttt
      361 ttccatattc ctcaacctca gcgggggttg aagacataag gaaaatccta tgcgttatgc
      421 tcaactggat ctgattaaat attctaatgt atttttctct accatcatta aatggttaat
      481 agccaaaaat caccactttt caatggctcc ttgttgtata agtataacta gaagatgaac
      541 acgtctgaga aattatagaa gctttttacg gtaattactg gggctgtgtc cagtagtttt
      601 tttgaagaga acagaaaaat agctcacgtg atctattcct actttttgac taatttcatc
      661 aatagataag aatgtgtctt tcagtagagc acttgctctt ttgattcgaa agtctagcaa
      721 gtaatcatga aaggaataac ctgtttcttt tttgaaggcc cgtcctaaat aagcgggatg
      781 gtaatggaat tgtgtagcta catctttgag cgtaagcttt tcactataat tgttttgaac
      841 gtaatcaatc acttgttgaa ttgactcatt aaaagaatca tcagttagag agttttgatt
      901 agctgctgat ttacgcattt tttcttcttg tactaactta actaaataag gaaaatcaat
      961 gggtttcgtt atgtaacttt taatagataa atcaatcccc ttttttgcat actgaaaatt
     1021 ttggtgggca ctaatgacaa tactagtaaa gtagcgattg gatgtttgtt gcaaagattc
     1081 gattaattcg agtccagaaa ttttccccat cgtaatatct gtgataagta aatcaaagcg
     1141 ctggttttgc aggtttttaa atgcttcgtc tccgtctgtg tagcagtgat cgataatgat
     1201 gtcggggact ttcttcaaaa gataatgcct tatgccttct acaattgtac aatcatcgtc
     1261 aacaattgca attctcaaca tgcatattcc cccctttttg tattttgata gtaatcgttg
     1321 ttccgacgtg tagttggctt tctataacaa aacgtgtatc tccaggatag agatgcttta
     1381 tacgtcgaaa agaatttgag atgccgatat tctcactata aggaatatca tcattaagtt
     1441 tttgttgaat atgctccagc ttgtcttggt ccattccttt cccattttca acgattttta
     1501 acacataatg gttggttgtt tcagttgcat agacagtcaa acgattttcc ttttttgatg
     1561 aatcgaagcc gtgtttaata taattttcaa caaatggctg taaagaaagt tttggtaaca
     1621 ttaattgatc aatatgatca atcagctgat agtcaaagtt atgtcgataa cgacgttgat
     1681 gtagttgcaa ggagaggtcg atattaatta attcctcttt caaatcaatt atgtcgggct
     1741 gatatgtatt attgcgatac aacttgctca ataaatatat actgtcgctg atttcttcgt
     1801 catcattcaa tatggcagtc attcgaataa attccaaatt attaaataga aaatgaggct
     1861 cgatttgata ttgaagtgct gcatactttg cttcttgctt caaccaatta ttttcataaa
     1921 gtgttttttg atatgtagca ttatctaaat aaaactgatt gatatgcata gcgatttctt
     1981 tcaaatcacc gactttgttt tcgatattga ttgacttgta gtctcctgta gatgaatgat
     2041 ttaaggaagt aatgatttcc tgatactgct ggatatagtt acgaaaactt ctatttaata
     2101 attgatgaat agcaagaata aacaaactca ttaataagaa aatcatgatt gccagaccta
     2161 ataaggtttg tgacgaataa tttggatttt catatacttg taatgtgtgg ttaccgatag
     2221 ctatctgttg cgtttttttc catttagaag gcgagatgtc ttcagataaa ttgacggaat
     2281 caataccatt agacaaagaa taattgtaat gtgttggaaa gagactcttt tttagcgcgt
     2341 tgatcgaaac gtctagaatc aatgtagcat cgggcatgtc ctcactaatg aaacttaatg
     2401 aacgaataaa agaaattttt tcgttttttg taggtgcgta ggcacttaag tatttttcgt
     2461 aatctaatga gatagatata tttttaactt catcatcttg cacaagtttt tttaagttat
     2521 ctactaaata ggtaggggag cgatcctttg acaaaatatc ttccatgtat tcagcaggtg
     2581 atgagaggta gtagtcatta aggttttctt ggaaatcata attttgatag atctctgtca
     2641 cgataccgga gagaatacga ttatatgttt ccatatagtg ggtgatttgc tgaatgtttt
     2701 gcaggttttg atttttttct ttttcaatca cattgatata ggaaaagaac aatgtgaaac
     2761 aggcaagtaa agtaataaaa aaaatcaata tgtatgtaaa acgcataata acttttgata
     2821 aatcgttttt cattcaaagt cctcattttg ataatctcta attttatgaa tcattatagc
     2881 acatattaca agtgatgtaa acgctttgca aacagggcta cttatgattg tgaatacagt
     2941 ttccgtttaa aaactattct attgtagagg tttaataaac taaaaataat gtataaaaaa
     3001 ttaatgtaag cgattaacca agtgggtata atcaaggaaa cgctgagaga ggagagacaa
     3061 aatgaagcta aagaaagggt tattaggaaa tctaaaaaaa aaccgagcat tgtttattat
     3121 gcttttacca ggtgcgttat ggttatttat attttgctat ataccaatgt ttggagttgt
     3181 aatcgctttt aaagatttca gaatttcatc aaggggattc tttacaagtt tgttcaacag
     3241 tccttgggtt ggctgggaaa atttcaaata tctctttgct tcgcaagatg cttggatcat
     3301 tactcgcaat acggttgttt ataacgttgt aggtattttg ctaagtactt tttgtacagt
     3361 gtcattagca atcattctga gccaaatggc gaacaaacgg ttattgaaaa agatgcagac
     3421 atttatgatt tttcctaatt ttttatcatg ggtagtcatc agttactttg tttacgcatt
     3481 tttgagtacg gataaaggat taattaatat tttactaagc aattttggtg tggaacccat
     3541 aaattggtat agtgaaccga agtactggcc ttttattatt gtttttacga gtttatggaa
     3601 aggcgtaggc tatggatcca tcatttatta cgcgtcaatt atggggatcg atactagtta
     3661 ttatgaagct gctttagttg atggtgccag taaatggcaa cagataagga atgtagtctt
     3721 gccacattta agatcgatta ttgtcattat gttgattttg tctgttggta gtatttttcg
     3781 agctgatttt ggattgtttt atcaattgcc aagaaattcg ggggctcttt atgatgtgac
     3841 gaatgtatta gatacttatg tctaccgtgg attaatgaat agcggagata tcggaatgtc
     3901 tacggcagcg ggattatatc agtcatcagt tggctttgtc ttgattttga ttactaataa
     3961 gatagtcaac aagttagatc ctagttcagc actattttag gaggaatcaa aatgatatca
     4021 aaattaaaac ggcagatgta cttttcgctg ccagtcgaac ggtttttcac aatattacta
     4081 atcttattta ctcttagctg tatttttccc tttattttcg taatcgtcat ttcattaaca
     4141 gatgaaaaag cattacaact caacggttac gaattattgc cggctcaatg gagcactgat
     4201 gcatatcaat acttaataca agatggcgga cagttacttc ggtcattagg cgttacaatt
     4261 atgattacag tgattggaac gttgattact gtttttatga cgggaaccta tgcttatgtt
     4321 ttatcaagag cctctttccc ttaccggaag ttctttactt tttatctttt ttttaccatg
     4381 ttatttgcgg gagggatggt tccttcttac ttagtgatga cgaagatgct tggcttaaaa
     4441 aatacaattt gggctttgat tctacctctt gcttttagcc catacaatgt cattatttta
     4501 aggacattct ttaagaagtc gattccagaa tcgattatcg aatccgctaa aatggatggc
     4561 tgtagtgagt ttcgagtttt tttccaaatc gttttgcctt tagcgatccc aggggttgca
     4621 acaataggtt tattttcaag cttaggttat tggaatgact ggttcaacgc attgctgtat
     4681 attgacacca acaaattgat tccattgcaa tatttgttaa tgaagattca aaattcaatg
     4741 gaattcttag caaataataa tgacattaca ttagcgcagc aacaagcaat tcaaaatagc
     4801 cttcctcaag aatcaacacg aatggcaatg gtcgtagtag ctactttacc gattgcaatt
     4861 gtttatccgt tcttccaaaa atactttgtt caaggattga cgatcggtgg agtgaaagaa
     4921 taaaaaagaa taggagatgt taaaagatga aaaagaacat gcagaaatat ttgattggtg
     4981 gtgcttctgt actaaccctt ggcctattag gtgcttgtgg tagcagtaat ggcgaagcaa
     5041 ataatgaaac aggaaccaca aaagttactt ggtatcaggt tggagaagaa cccaaagatt
     5101 ttgatgaggt catgacaaaa gtcaacgaaa aattaaagga agaaatcaat gtagagttgg
     5161 atatgcggct gattccggac ggggattatc agcaaaagct tggtgtgatg atcaattcag
     5221 gagaagaata tgatatttgt ttcgtcaatg gcacagatta tgtgaattat gggaacaaag
     5281 gtgctttcat tagtctcaac gatcatttgg ataaagagtt gaaagattat gcagctgaat
     5341 tgaatgaagg atttattgaa ggtggggcaa ttaatggcga aacttatgga ttgccagtca
     5401 ataacgatat tgccacaacg gaatattgga cctttaataa agatttggta gatcaatacc
     5461 aattggatgt agagaatgtt gaaggtgtag aaacattggc tccagtatta gaaaagtttg
     5521 cagaaaaaaa tactgatacg aatattacgc caatcggtat gacccgtacc agtgtctgta
     5581 ttccttatga caatattatc ggtggtggtg taccgctagc ggttgcattt gaaggcgata
     5641 cacaaacagt tgttaatact tatgagactc cagaaatgat gacttatttg aaggctatgc
     5701 atgagtttta taaaaaagga tatattttga aagatgcagc cactgttacg acagatccat
     5761 ttcgaattga tagtaaaaac tggtttttag gtcgtaactc aagtggttat caaccattag
     5821 cacaaaaaaa attagaagct ctagcgggac atggattaat tttaaaacca gctggaaaag
     5881 cttttaaaac gactggattt gcaagagtgg caatgcatgc ggtatctgct tcaagtaaac
     5941 atccggaagc agccttgaaa ttgttgaatt atgtgaatac caatcaagaa gtagcggatt
     6001 taatggttta tggaattgaa ggaaaacact atgatcgcaa tgaagatggt tcgattactt
     6061 taacaaactc tgatgattat tatcaaggcg cttgggcatt ggtaaatact tatctagttt
     6121 caaaaacttc accgattaca gaagaaagta aaaaactaga agaagaaagt aaaaagtttc
     6181 aaaatgaagc accgatgtct cctattttag gtttctcttt tgacgcttct gattttcgaa
     6241 cagaaattgc aagtgtacaa aatatcgtgt cacaatatga agtactttta agctcgggaa
     6301 cagttgaccc tgaacaatat gtaccagagt tgttagaaaa actagaaaaa gctggattgg
     6361 ataaaattca ggcatctgct caagaacaat acgatgaatg gcgaaaatct aacaaataat
     6421 tttttactaa atgaaaagaa tagactaagc agttagtggg gggattcaaa tgaacgaaaa
     6481 caaatggacc aaaggattta attgggtttt tcaattgttg ttactaaact ttacttattt
     6541 tatactaatt tgtttaggat ttggaatttt aggttttttc cctgctacga tagctttgat
     6601 tgctggcgtt gtgaatcgaa aaaaatatgg cttgcaagag tctgtgaagt tcatgagtaa
     6661 aatctataag aaaaattttg ttagatataa tctatattgc tggtcagcaa ctttgttgtt
     6721 ggccggcaat attactacga tctggctttg ttggcagcaa agcgcaccat tgttttattt
     6781 attaagtaca ttattatcgg taattacact gttcctagtc tttgcaatgt tgaattgggt
     6841 cgtgatcaaa agtcttgggt tgtggatgat gggagaccga ctgattgact atttgctttt
     6901 cacagcaacg atgttgccaa cggtagtcat gcaaggcttg gtgcttggaa tttatttccg
     6961 aatattgatg attttgcctg tgatttttct gtttggcgga acagctgtaa cggtaatatt
     7021 gattagtaaa ttaactgaaa gagcgttagc aaagttgcct tgtcagatac aatcgcacta
     7081 attttttcac aaaaaggagt atttctttct atgaagttaa ctgataaaca agtagctaat
     7141 atagccttta tccagcaact tgagattcca ttgacacaaa aagatgaaga acaaatagac
     7201 ttgattttta ctgacacaaa ggctaatgtg cgacaaatca agattactaa aaatgagcag
     7261 caacgaattt gcttgtctta ttcacatgaa gacttgctgt atcgaggatt gtttatctgc
     7321 cttccacgtt ttttagctca aggaggagag atgccataca ttgaagaaac ctcgatcatt
     7381 gagaccagtt attcattaga tgcttctcgt aatggcgtga tgaataaaga gactctaaaa
     7441 aaattcattt cttacttagc gttgatgggg tacaacacat tgtatattta cacagaagat
     7501 acctatgaag tagaagacct tccatatttt ggttaccttc gcggagcgta tacaaaagaa
     7561 gaagtgaatg aaattgttga atttgcttct ggttttggca tagaagtagt tccatcgatc
     7621 cagacccttg ctcacttgac ccaatttttg aagtggtatg ctcaacaaga actgatggat
     7681 gatcaaaata ctctactgat cggtgaagaa aaggtttatg cggcaatcga aaagatgatt
     7741 attgcctgca aagaaatgta tcagacagag cgtattcatt tgggaatgga tgaagcctat
     7801 caagcaggtc tgggacgata tttaagcctg tatgggtatt gtgatcggac aactttaatg
     7861 attcaacata tggaccgtgt gttatcaatt gttgataaat ataagatgaa accaatgatt
     7921 tggagtgatt tcatttataa gctattagat gaaaccggta aagataggta ttacaatcct
     7981 gattttaaac tcaaagcatt agcagctaaa ggattaccag caggattaac ctatgttcat
     8041 tgggactatg gttcagatga agtttcctcc tatgagaaag taattgataa ccatttgaaa
     8101 ttttgtgaca ttgatcatta cgttttagct agtggtgccc atatctgggg gagactagcc
     8161 ccgaatcatg ggaaaacaga agcaattgtt tctgcagctg taaaagcttc taaaaataaa
     8221 ggtgtacgtt ctgtgatgtt aactacttgg ggagatgaag ggcaggaaac agagcattgg
     8281 cattccttaa ttggggcatt aaaattagca gattatgtct attctgaaga agagtggaat
     8341 gattttgagg aaatctacga tcgtttatta ggaacaggta gttatcaatt aatgtccgaa
     8401 ttacgatatt tcgatgaagt ggataccatt acaaataaca acttacaaat ggcttccgtg
     8461 agtaaactgc tactttggca agatccgtta gttggtatat atgactttca tgtagccgct
     8521 cataacaaaa ttgccactag aacaattggt acttattatc atgagttagc tcagaaatta
     8581 gatatgaata aaaattggaa tccaaaacaa ccaattttaa caactattcg tcaacgatat
     8641 atacttttag ctgaaatttt agcacaaaag gcagaactag gcatctattt acaacactgc
     8701 tgtctggtag aagaccttgc aggattaatg gaattagcga atcggtgtga agcactagcc
     8761 aatcgagtaa atgaattggc atctattcat gaaataattt ggtatcaaac atacaaaacc
     8821 tatggttggg aagtattaga acaacgatat gctgcattag ctcatcgtct gaaaaccgtt
     8881 gcgcgtcgta ttaaaaatca tcttcagggg caagatgccc tagaggagct tttggaagaa
     8941 cgattaccgt tttgtcaata tcaagtgccg cttgaaattt ctggctttaa ttatcggcaa
     9001 agctcggtgt caggctacaa ttaacgaagg aatcttcccg ttgaacggac cacttgctca
     9061 tctaatggga aagtggttcg ttttctattc ataagtagct taccactaac tcttttatta
     9121 agaagagaaa aaaagtagga agtgagaaaa tatgttatcg gactattatc aaaatctgtc
     9181 agttataagt gtcaatacag aaccattacg taattattat attccttatg caagtaaaga
     9241 agaagcaaaa caagagttag aaagggaaaa aacgagccag tttatcgatc tcaatggtac
     9301 atgggatttt cagtattatc cttctgtcca acagttaccg gaaaattttt ttcaagaggc
     9361 ctttactgaa agctatgata gaatttctgt tcctggagtt tggcaaacag agggatttga
     9421 cttacctcag tatatcaatg tatcttatcc aattcctttt gatccgcctt atgtacctaa
     9481 tgatacccct tgtggtgtat atcggcgtca gtttcaaatt gagcaaacag atgcacgctt
     9541 ttacttaaat ctagaaggag cagatagtaa ttgttttgtt tggataaata gaaactttgt
     9601 aggctatgac caagtgagtc atagtacatc tgaatttgat gtgaccaagt atttacaggt
     9661 tggggttaat gaaattcaaa ttctagtgat gaagtggtgt gatggtacct actttgaaga
     9721 tcaggataag tggcgaatga gcggtctatt tcgtccagtg tatcttcttc aacgaccaac
     9781 tatgcatctg acaagttatc aactacggac gttgactgat cctaaaaaag gaagcggaac
     9841 attaatggtt tcttttcaac gatttactcc tattgaagta tcgttaccta tttcttacga
     9901 attattccat cctgatggac gtctcatagc gaaaggggag tgttactcgg aacaacaaat
     9961 aaatttggat gttgaagatt gccttttttg gacagcagaa acgcctaaat tatatacatt
    10021 acttttaaca tgtaatggtg aatattttaa agaaaaagtt ggctttcgag cactgacgat
    10081 caagaatcgt aagttattgc tgaacggaac acctattaaa ttatatggag tcaactatca
    10141 tgacgtgaat cagcaaggca cgccagtgat gacccgagag cagttacgtg cagatttgtt
    10201 attaatgaaa aaattaaatg tgaatactgt acgaacggca cattacccta aatcaccaga
    10261 tttttatgaa ttggcggatg agattggtct gtatgtaatc aatgaagcag atttggagtg
    10321 tcatggtgtg gttaatctat atgatactga agcgaattat caactaattg ctaatgatcc
    10381 gacattcgaa aagccgatta tggaccgagt gatccgttct attcttcctt atcaaaatcg
    10441 aagctctatc attatgtggt ctatggggaa tgaatctggt tatggagtaa attttgaaaa
    10501 agctcaagct ttagcacgga aattggatga ttcacggttg attcataatg aacgctcgat
    10561 tgaaccagtt attgaccaag tgaatgattt tagtaacttg gatgttatta gtcaaatgta
    10621 tcccccactg gaagatgttc atcgtcattg tcaaagaagt gatactgaaa aacctttttt
    10681 tatgtgtgaa tatagccacg ctatgggcaa cggtcctgga gatcttaaaa cgtacttcga
    10741 tttgattcaa cagtatgatt cctttcttgg cggttgtgtc tgggagtgga ctgatcatgc
    10801 aatcaataga ggaactttag aaacgccatc ttatttatac ggtggtgatt ttggtgaaaa
    10861 atatcacgac ggcaattttt gcgtagatgg tttattgacc ccagatcggc aattaacagt
    10921 aggtgcattg gagtaccgta atattcattc gcctattcaa ctgattgctg tagatgagga
    10981 gctgcaagaa gtaaccttga gcaatataac tgcttttaca gccggttcag catttaacct
    11041 ttctcttgaa tggtatatag atggaaaaaa aacagtttat gaagtaatga atcctttcat
    11101 acttgagcct ggcaaaagtt gttcattgcc catcacagta cctacaacga ttcatggtgc
    11161 tcatacggtt gctgtttcgc tatctgttta taaaaatgat gagctttatg gacagcaaca
    11221 gctctttctt aaaaaagaaa aaatgagatt gccaagaact gctcatcata atgatgcacc
    11281 acaaatttca gtaaaaggca cagagctgat aatcaaagga gaatccttta tttatatata
    11341 tcaattagca aaagctgcgt tcacttctgt aaagatcaat caaaaggaac ttttggaacg
    11401 tcctactcaa ttttcaattt ggcgtgcacc aacggataat gatcgtaact tagtaaacaa
    11461 atggaaagca gctggatatg ctgaaatgtc tttagaaatc agaaaaaaag actggaaatt
    11521 cgtagatgat catctcctga tcgaaacgtc ttatcgagga gttattccag gattacagcc
    11581 aatttttaca atgcacatac agttgaaaat aaatggtgca ggcgtaatca accaaaaagt
    11641 atcagtggag aaaaatccga tttttccaga tttccctcgc tttgggatgg actggccgtt
    11701 gaatgatact ttttcgaagg tttcttattg tggctatggg ccgtatgaaa gttatcctga
    11761 taagcactat gctgcgatgt ttggtattta tgagatggag attgaggaat ttgatcagcc
    11821 atatatcaaa ccacaagaat atggtaaccg ctggcaatgt gattgggtat caatcaaaaa
    11881 tcatcaagga tctaccatga cggtttatgg aaactctaat ttttctgctc tgccttatag
    11941 tcaagctcaa ttaaccgaaa agtcacacca ttatgaatta ataaaagaaa atagaacttg
    12001 gttcaattta agttataaac aaaatggtat cggctcacac agttgcggtc cttatttaga
    12061 agatcgtttc cgttttatgg aagatacttt tgactggaat atagtgatta ctttcaaagg
    12121 agaaaataga tgaaagaaac aaagatcaat caagtcaaaa aaaaattatc tcgttatcaa
    12181 gaagttgttt atcgaaacca ttgcttaaaa tggtcaaaaa ataagctgaa tgttcgtttt
    12241 aatggattat tttatcgttt cgaacagtta gaagaagtct ggaaaccggt aacgaatttc
    12301 caatcatgtc ctgtacagct cataaatgat ccaaaagaaa catacgattt atgtataaca
    12361 cacctttata accagcattt gaaaaatgac ggattttatt taaaggttac taatgacaat
    12421 caacttacga ttgaatcagc aaaccttcga gggtttcaat atgctatgga agccttatta
    12481 gagagttttt tttcggtagg agatcaatta ttattaccgg aattgattct gaaacatgaa
    12541 ccatcagtga aaattcgagg aatcattgaa gggttttatg gtatcccctg gacacatgaa
    12601 atgcgcctct ctcttttttc atatatgaaa gataaccaat tgaatacttt tatgtatgcc
    12661 cctaaagatg atgaattatt acgtaaaaaa tggcgtgaac tctacgatgc gcaagaatta
    12721 gacaaattta aggagctttt atctgctgct gaggctagca atatcgattt ttggtactta
    12781 attagtcctg gaaatgatat tgatattacg tgtgaagaag acattcaagt tcttctgaaa
    12841 aagttagaac aactgattga gcttggcgta tttcaatttg gtctattaat ggatgatatt
    12901 gactatcaat taaaaggtgc tgctaaacgc cgttttcgag agccagcatt cgctcatgcc
    12961 tatttagtga atcgagtaga ggaatatctt agcactcggt tgtccaatca tgaattagtc
    13021 atttgtccta cagaatatga taatcgtcat ggttctcgct atttagcaac gttaagtcaa
    13081 gaaataccag aacaattacc gttcttttgg actggcccaa gcacgttagc ggcgtcaatt
    13141 tctacagaag agctgcagga aatggcttct gtctatcaac ggccgatgct gatttgggat
    13201 aatattccgg taaatgatta tttagaggat aaagaactat tgttcatgag tccatatgag
    13261 aatcgaacac cgaatctttc taaggagaga taccaggtta caggagtagt ttcgaatcca
    13321 atggctcaat tagaagcttc aaagtttacg atcaatagta tggctaatta cttatggaac
    13381 tgtgagcgtt ttgatccttt ggaaacttgg acgtcagtcg ttgaaaaagt agttggaagt
    13441 aaactacaac cagcttatct taccttaacc aatgcttttc caaatcatta cacaagcagt
    13501 ctgctttcag atgaagaatt actgctagcc aaagacccag aatgggtcac aaaagaaatg
    13561 gctgctttag tccaagcagt aaaatggatc cagcaagaac aacatacatc tagattgaga
    13621 acagaattgg agccttggtt acaagcaatc acagaaagtt ggactttctg gcaagaatgg
    13681 caattaaaaa aagatccaaa agaagcagaa gaatggttgg ctaagaaaaa aacacaccgc
    13741 ataggacgtg atttagtaac tgcccattta gagcaaatta tcaaaagtta gatatcttaa
    13801 aaaataaaaa gcaagaaaaa ggagaacaaa atggtcaaga gattaattag tgcaaactac
    13861 agtgatgtca agaaaatgac gtcaaaagag ttgaagcaat ccatcaaagc gagtgaagga
    13921 cgggtgattg tttgtgaaac ggttgctaat actcttccac aatctggaga aattacgaat
    13981 gcagaagttg cgtcagcatt tggtgcagat ttgattcttt taaatggttt cgattgttat
    14041 cgtcctgcag tttttggact accaaatgtg acgattgaga aggtttttga tctacagcaa
    14101 acgattgaaa acccgattcc taaattaaaa gaactagtcg gtcgtcctgt gggaattaat
    14161 ttagaaccca ttgcccctaa aacaaatatg atgagtgaga aagtagaaat cagccaagga
    14221 agagcaagct ctgttgaaac gatacaaaaa gcagaagaac taggagtcga ttttatttgc
    14281 tttaccggta atccaggcac tggagtaacc aatttagaaa ttactaaatc cgtaaaaatg
    14341 gcaaaaaagt atttttcggg attgattatt gctgggaaaa tgcatagcgc cggctcagaa
    14401 gagccagttg tgaataaaga ggcgattatt tcttatgcgg aagcaggcgc agatatttta
    14461 ctagttcctt cagtagggtc tatcccaggc tttttagagc aagacctaat tgaagcggtt
    14521 cggttggcaa aaaaatatga tttgttaacg atgtctgcaa tcggtactag tcaggaaagt
    14581 gcccgaccag aaacattgcg gcaattagcc attatgaata aaagttgtgg tgttgattta
    14641 caacatattg gagatgctgg atacggggga ttggcaccag cagaaaatat tttcgaaacc
    14701 tcggttgcaa ttagaggaat gcgacatacc atcaactgcg ttagccgctc gatcaatcgc
    14761 taacaaaatt aaataaaaaa tggttaaatt gattatttaa gtgggaacat acttgagata
    14821 tcagctaact tctgtcactt taaaaaaggg actgtgacag aagtgaaaat agacaaaaaa
    14881 tatccgaaca agagactagg atagcagaaa aactatcgtt ttgcaacgag gaccaactgg
    14941 accgtaggag caattctctt attgttcgga tatttcgctt gttcaaggac ttatgtctgt
    15001 agtgcccttt tttggtagga aaaatggtag ggaaccatat catttttaat gaaaatcaag
    15061 catttcaaaa gt
//
LOCUS       DAZUKZ010000061        14373 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000061 DAZUKZ010000000
VERSION     DAZUKZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.062"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..245)
                     /locus_tag="ACG8EF_001784"
     CDS             complement(<1..245)
                     /locus_tag="ACG8EF_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947091.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVV"
     gene            391..2028
                     /locus_tag="ACG8EF_001785"
     CDS             391..2028
                     /locus_tag="ACG8EF_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947092.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2173..2913)
                     /gene="truA"
                     /locus_tag="ACG8EF_001786"
     CDS             complement(2173..2913)
                     /gene="truA"
                     /locus_tag="ACG8EF_001786"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS4947093.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3104..3739)
                     /locus_tag="ACG8EF_001787"
     CDS             complement(3104..3739)
                     /locus_tag="ACG8EF_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS4947094.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(3886..5106)
                     /gene="sstT"
                     /locus_tag="ACG8EF_001788"
     CDS             complement(3886..5106)
                     /gene="sstT"
                     /locus_tag="ACG8EF_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFS4947095.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            5325..6323
                     /locus_tag="ACG8EF_001789"
     CDS             5325..6323
                     /locus_tag="ACG8EF_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947096.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            6519..8354
                     /locus_tag="ACG8EF_001790"
     CDS             6519..8354
                     /locus_tag="ACG8EF_001790"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4947097.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8414..9130)
                     /locus_tag="ACG8EF_001791"
     CDS             complement(8414..9130)
                     /locus_tag="ACG8EF_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS4947098.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(9357..9899)
                     /locus_tag="ACG8EF_001792"
     CDS             complement(9357..9899)
                     /locus_tag="ACG8EF_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFS4947099.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9904..10131)
                     /locus_tag="ACG8EF_001793"
     CDS             complement(9904..10131)
                     /locus_tag="ACG8EF_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFS4947100.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(10210..12090)
                     /locus_tag="ACG8EF_001794"
     CDS             complement(10210..12090)
                     /locus_tag="ACG8EF_001794"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4947101.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(12279..12893)
                     /locus_tag="ACG8EF_001795"
     CDS             complement(12279..12893)
                     /locus_tag="ACG8EF_001795"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4947102.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            13035..13595
                     /locus_tag="ACG8EF_001796"
     CDS             13035..13595
                     /locus_tag="ACG8EF_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4947103.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13900..14214)
                     /locus_tag="ACG8EF_001797"
                     /pseudo
     CDS             complement(13900..14214)
                     /locus_tag="ACG8EF_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT         4506 a         2766 c         2250 g         4851 t
ORIGIN      
        1 aaaacgactg caggaattat tgtaagaaaa ggataaaaat agttcagtcc atctccataa
       61 ttgttaaatg ttgtatagtt gattggccct tcaaaaatag aagataaccc tttgattcgt
      121 gaaagatgaa aagataaatc acctgttgta taaatttctt gagcatttct cataaaattt
      181 actgaatata aagtactaag taagataaga actactaaaa gaaatgcttt tttacttatt
      241 ttcatagatt tgttgttctc cttttttaat tctattttca gtatactaga attgtctttt
      301 tttaataagt tttgcgttaa atttttcata gcactagtat gatatactag aaaagaatca
      361 atttttatta cttttaaata ggagaaaaaa atgaaaaaaa acaaaaacca aataatagat
      421 atcattttta tgttatttct aagttttgct tacgtcatcc ctctattcat gtctaaggga
      481 atctatcata gtgtcaatca ggatacttac tttcatttat ctcgaatcat aggactagat
      541 aatgtatgga gttctccagt aaacttcaat aacttcgatc atcatggaac aatgatgaat
      601 attttttatc catggcttac tctttatcca gcttttttat tttataaaat gatgggaaat
      661 cttgttttaa gttataatat ctactatttt tttatcactt ttttaacaat ggtcgtttct
      721 tatttttcta tgaaacaaat aaaaaacaat cgttatattt cgctgttatt ttcgattatt
      781 tatacatttt ccgcctatcg tgccattgac atattccatc gtgcttcatt gggagaagct
      841 gtcgctttaa catttttgcc tttgatacta atggggtgtt acgaaattta tattagagat
      901 taccaaaaat ggtactggtt gtctattggc atgacgctag tagtgtatac tcatttactt
      961 tctgtcgcta tggtttctgt atttattgga ggaacgttgt ttctttcttt ttatttttgg
     1021 gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta ccgtcctcac ctttttttta
     1081 agtgcgggat tcttaattcc ttttattcaa caatctagag cacaagaatt gaaagtccca
     1141 ctgggaaaag aattaagtgg tatggctcct agtgatatgc tgacacatat tttaaataat
     1201 aactataaca actatactat tggattattc ttatttctcg gattgattgg tgcttttatt
     1261 tttataaaaa aattaactgc tgatgattta tttattttct ttcttggtgt atttgttctt
     1321 ttttgttcaa ccaatttatt tccttggcaa ctttttaacc atacaccagt taagagtcta
     1381 caatttgtct ggcggttaaa tggtttttct tctttattta tagcctatac gatgagtata
     1441 gtgatctatt atattttttc tactaaaaat aattcatgga aaataatggt ctttacgttt
     1501 cttgcactaa tattgcatct ctcaggagta agcaattcta tccatgctaa caaagattca
     1561 ttaacactta tagcacctga agacgctgtt gctatagctg aaaattacaa tcatactgat
     1621 tatgcaaaca aagagtcaat ctatcatcct gatatgatta ataatgatca atatttacta
     1681 ggtaaccagg aaataaatcc aacaactcat tttatgagta acaaacttac aattgaatta
     1741 gataattcga ataataaaaa tactgtcttg acgacaccta tttatcgtta taaagggcaa
     1801 gtggcttcta ttaatggcaa actagtacaa acaaaattat ctaagtttgg cacaactgaa
     1861 ttaactattc ctccaggtat aaataaagtc gttattactt atcagtacac aaaactagca
     1921 attgcttcaa gatatttatc tatcgttact ttgattttat ttttacttta tcgatttact
     1981 ttttctaaac aaaaacaacc gagaagagaa gtacaacatt ctcactagat ttttatttaa
     2041 gaaagacttt ttagctgtac acataaacag gctaaaaagt ctttttttat atttctttaa
     2101 tttgcttcat acattattta acatcgatat ttatactgtg aaacatgcgt ttcatccatt
     2161 atcaaagtat ttttatcgat aataaacttc atccaaaaac agaccttgtg cagggacggt
     2221 ttctcctgca tgttgccgca ctttgctttc aaatatttca tcgattgttt ctatcggcat
     2281 ggcacctgtg ccgatttcta gtaatgtccc catgataatc cgcaccattt tatataagaa
     2341 tccgtctccg acaaaagtaa aatgaagcat ttctcccttt cgttcgatag ataattcatc
     2401 aatcgtacgc actgtggatt tttttgattt tttcaatgca gaaaaaccaa tgaaatcatg
     2461 tgtacccact aatttttcgc aagctgcatt cagcttgtcc atatccaact cttgagggaa
     2521 ataaaagctg tggtttcgct caaatgctga aggaatcacg ttgttccaaa cataatagct
     2581 gtattttttg cccaccgcat tatatcttga atggaagcgc tcaggcattt cttcgattct
     2641 tttgatgaca atatcccgcg gaaggtaacg attcataaag tcgatcattt ctgccagtcc
     2701 aatttctgtg tttgttttaa aatttgctac ttgtcctcgc gcatgggtgc cagcatctgt
     2761 tcttcctgat ccaataattt ctatctcttc atttgtcatc tgatgcaata ctttttcgat
     2821 ttttccttga atggttttat cgtcatctcc aagtctttgc catcctgaat atctttttcc
     2881 gtcatattcg attgtcaact tgatgtttct catattttag tggctcctct gtcttccatt
     2941 tggtcttctc tctacctttt taagattgta gcacaaaata tagctccttg cttctctaag
     3001 acagaagcta tacaagaaaa ctgtggaaat atatatttca gaaaaacaaa aaacagccac
     3061 cggatgaagt atccgctgac tgttcaattt gacggctcaa gttttactgt ttgagatgca
     3121 agatactcaa cctggcaatg ggcacataat taaagaaaaa actgattctt tggcaaaaac
     3181 ctattttatt cagtaatggc caagaattta tagaaggaat tcttgccagt ccataaatcg
     3241 ccgcaaaaag caaactgatc acagacagtc caaggtataa gtggcttacg tccatttgcc
     3301 cattttttcg atagatcaag aaaagaagaa ctggaaacaa taaaaaacca ctgtaaccta
     3361 aacctgatcc gatattatgg acccatgtcg agacattcca cgaagattca tgggtattga
     3421 tactgaacaa acctgtaaag atacaatcac ctataccata gaacgcgatg gctgctgtaa
     3481 ctagggtagc taacacacga gatgtactaa caaatgcagc atacaatgca ggcaatgaca
     3541 ggatgaaaaa gatccctgaa accacagacc aaataagaaa cgcttttctt acaggactgg
     3601 aaacctctcc aaatacactg atcacgctct tcatttgatc taattcagga taatacaatc
     3661 ctaatacgta cggagtcaaa aaatcactca gcacgcctaa taataaaaag aaaaaaccaa
     3721 atttacgtaa aaaatccatg atacgcctcc aataacaatt acctatgctt ttaattgtta
     3781 ttataaacga ctttatcttg atttaacaga aacctctatt tacacgaaca tctatataac
     3841 tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct aacgttcaac gttcacttaa
     3901 tccatctaaa caatctttca atttgatttt ttccccactt cttttacgcg cgcctagctc
     3961 tcctattgca gtaaacaaca gatcacttga cgaatttaag gcagtttcca ttgaatcctg
     4021 gataacaccg ataataaatc caataccgac gatttgcatc gcgacatcat tagagatacc
     4081 aaacaaacta catgctaatg gaatcaataa cagtgaaccg cccgcaacac ctgaagctcc
     4141 acaagcagct aaagcagaca aaatacataa aaggactttc aaaacaaaag gtgcttcaat
     4201 ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg gtaatcgcgg caccgcccat
     4261 attgatcgta gcacctaacg ggatcgatat ggaataagac tcttttgtca atttcaatct
     4321 ttcagccaac atcatgttta tcgggatatt tgcggcagaa cttcgggtaa agaaagcggg
     4381 aagtgcgctt tctttcaaac aaaagaaaat caatggatag ggattttctt taataaacaa
     4441 aaacgtgagg aatgggtaca caactaatgc gacaaacagc atcgtaccga tcaaaacaag
     4501 taaaagctgc agatactctg ccaatccagc gattccagtc gtagcaattg attgataaac
     4561 caatcctaaa atccctatcg gagcaaatgc aataatcact tgtacgactt gtgacaacgc
     4621 tatagatatc tgatccacca actcttttgt cgattctgaa cgtgctcgaa aagctagtcc
     4681 caacaataca gcccagaata agacgccaag gtaattcgca tcaataagag attgaacagg
     4741 attttgtgtg acattcgtca acatagtaga taaaacactt tccaaacttt gcggtgcttt
     4801 ttcagatact gtttcttgta aagtaatagg aacagtaaaa attgaactta caataactgc
     4861 tacaagtgca gaaagcaatg ttcccactaa atataagatc aaaataggct tcacgaatgt
     4921 cttattccca attttatgct tggcaattga cgccatcgtc aagaaaaaaa ccagaatagg
     4981 tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat attgaaataa atgtccatgt
     5041 ggggaaaaag ataccaaaca atattccgac taaaatacca acaacaatac gttgaactaa
     5101 cgacatatcc aaaaacttct ctaacaaatg accacccttt cacaatttta acaatcattg
     5161 tatcatttta tgcaaagaaa ctaaaggtat aattttctaa aaattctaac aatacaaaca
     5221 accatttctt gctaattttg tactttttct gtctacacgt tgttttttga gatattctaa
     5281 gttaggatgt tattaatggg caacttatca ggaaacgttt tcttatgttt ttgtccaatt
     5341 tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga gagttatggc ttcctgacaa
     5401 tctttgtcgc ttgtataatg atggttttac agcgtatatt gatttcagca gtcactattc
     5461 ctacatatgc aacaaaccct ggtcgattct acatctatac atttttacaa acttccgttg
     5521 ttattggtgc aaatttgatc ccactttaca tgggttacca atatcaaaaa atcgcccgtt
     5581 taaaagtttt acattattta agtcgttttg cctttatatt cctaatagcc acactagttt
     5641 gcaatatctt tttttacgta caagctggca gcttgaatat tcgtgattat tggcttatcc
     5701 taacacctat cagtcagaat tactttactt atgctgtttc ttgtactttg cttttttgta
     5761 gtattcctta tacagttgga tttttaaata aattatcgaa aacaacaatt aaatatctac
     5821 tattattttt aacaattgga ttagttggct gctctactct atttaataaa gacccgtttg
     5881 cattacagaa tggacagagt attttatgga ttttttattt atttttaatg ggttatggtt
     5941 tgaaagaatg gaactggaaa gaaaaggtcc gctataagct tcctcaagta tttatcagct
     6001 tgattgtttt attcggtttg attattttga tgacacaaat ttcactcatc atcagaggaa
     6061 atacagatac agcgctgagg ttttctaaac catattctct cttcgcaatg tattatactt
     6121 tctcttcgtt tttattttta gaaatgctat ctgataaatt aggtataaaa ggaagaggaa
     6181 attttcttgc tgtatttttg attgtcactc aagttatatg taatttccct ttagtttctt
     6241 atcgcgtatc tacatttatg aaacgtgaat ttccaaactc agggaaggca tggctgataa
     6301 atatcgggca gttttttatt tgatatttat tagcagtgtc cctcctagtt atctttttat
     6361 tttatataca aaaaatccgt ttcattcatc gtttcatcga atacttcgct tttgattcga
     6421 ttgaacaatt aactcagcgt tggaaaaaaa gaattcattg gctttttgtt cataaaagtt
     6481 attttttagt tgcagcattt ttttactgct ttaccttcgt gcagattttc gttttagaac
     6541 caaaagaagg ttggaaagaa actatccagg ttgccttaaa aatatttact cagcgacaag
     6601 cacccatgat cctgacaatc atcattatta tgggattttt cttcttacta ttgttgattt
     6661 ccaatcgttt ttggtacgcc ttgactgcta ccttgatcat taacttactg ctcactattt
     6721 cgacagtaat caaaatggaa atgcgggaag agcctgtctt tccttcagat ttaaaaatgc
     6781 tgaccggatt gtcagaacta ttatcgatgg ttagcccagt cttattaatc gtgggcggat
     6841 tgatccttct cctattattg attactagta ttattgtaca acgtagacta caaaaacagt
     6901 attccttgaa gattcattgg aaacgtcgat ttatcagtat cgctgtatta ttaggatgtt
     6961 tttcgggtgt ctttttcatt aatcacaaaa attcgccttc tttcttgctg tttaatttat
     7021 ttaaagtaaa caagactttc tttaaccagc aaggcgccgt aaaagataac ggaccagtca
     7081 ttcaattcct aaacaatatc gacattaaaa tcatggaaga acctgctggc tattcgaaag
     7141 aaaaaattaa tggaataatg aaaaagtaca acacaaaggc agatgaaatc aaccaaacaa
     7201 gaagtgactg gttaaacaat cagacgatcg ttctcaattt aagtgaaagc ttcagtgatc
     7261 ctgcacgtgt ccctaatctg acagtaccta cagaccctat tccttacatc aaaagcatta
     7321 taacagaagg aacaggagga ttgatgttat ccgtcggtta tggtggcgga acagctaaca
     7381 tggaatggga aggactgaca ggcttgagta tcagtaacct gtctgcttcg ttagttacac
     7441 cttataccca gctagtggag cgtcagaaaa taagtccaaa tattaccgat ctattcgatg
     7501 aaaaaatagc catccatcct tttacagctg ctctttatcg cagaaaacaa gtgtttgata
     7561 aatttggttt tgacaggttc tattatttag acagtccgga taaattaact tatacggata
     7621 aaatagaaca aagcccacgc atatcggatg attcagcata caaagaaacc ttgaaagctt
     7681 tacaaagcaa tacggaaacg tcacaattca tccaattatc cactatgcaa aaccatatgc
     7741 cttatgatga ttactatagt gaattgaact acactgctga aggaactgct gtcattccta
     7801 atagaagaaa agaattacaa acttatatgc aaggccttca ctataccgat acagcagtaa
     7861 aaaaattcat ccaagaaatc gacaaaatcg ataaaccgat tacctttgtt ttttatggtg
     7921 accatttacc cgcaatctat agtgggaatg attcgaaaaa atatggatta gaacagcacc
     7981 agacagatta tttcatttat aacaacaagt atagtcgtga acaagcagct acgttaaata
     8041 aagaagttgt tgctcctaat aactttggtg ctatggctct tgagcatgcg aatatcaaag
     8101 taactcctta ctatgcgttg ttgacagaag tatctaatca tctacctgca ttaacaattg
     8161 atcctacaga aagcttgtct aatcgtttta atggaaaaca ggtctttgtt acagaggata
     8221 gtcaaattat ccaagaaaaa aatttaactg agaaacaaaa agaaattcta gaagattatc
     8281 gtctgataca atatgatctt gttgcaggta accagtacag tgcaacatgg gcgacacaaa
     8341 aaataaaaga ctaacaaata aactgagcaa ggggttgtga taaaagacat gtcacaaccc
     8401 cttgtttatc tcattaattt gcttgtccat aaacgatttg attaaagcca tttttcaatt
     8461 cattattcgc tgtctcgttg aagtaaagat aattatcggg attcccctga ttttgttgtg
     8521 tcaccaatac ttttggctga ttttgctgaa acccgaatag atagacgtgc tttttcagat
     8581 atggttctgt ctctgcatcg gaatagacgg ctgtgatttg atagtctgct tgacctgtcc
     8641 cactttctga ccattctgca gaaacaggca ctccaccaat agccatcttc cactccccgt
     8701 tcaaaatagc ttgagggact ttaagaccat ataagtccac actcatttga ttcgtataac
     8761 ttttgtattg ttgtcccatt gtttggcccc atttggtcat aaatgcctct aattgtgcgg
     8821 atttgtctgc attccacagt gtttgttcaa cttgttcgct gctttctgaa gaggaagatg
     8881 aagcctctac agaagaactt gaagtagatg tttgagttgt ttcagtcgtt ttggaagact
     8941 ctgacgaagt agaggtatct gctgtttctg tacttgctgc agttgacttg ctctctgtgc
     9001 tagttgttgt agtagttgca actgttgctt tcgctgtttc ttttgttgat tcttttttcg
     9061 attcattccc gttggtacaa ccagctaaca aaaaagctag accaattgtt actaaaatcc
     9121 tttttttcaa ttaaaaaact cctttcgatt actcccactt ctttattcta tcaaataagc
     9181 aatgtgattt tattgatttt tcgtattttc tcatcaatct ttcaaatctt ctaaaaactg
     9241 agaagcaccc gctgatgttg tctgtaatac atcagcgggt gcttctttta ttttaatctt
     9301 cgtgattttc caatttacct cacgctttat atttgtattt gaagaaacat agacttttag
     9361 tcatcatcgt cttcctcatc caaatcttgt cggacagttt ttccttggag tctttgtact
     9421 tggtagatga tttctttatt gaaacttaaa agttcaacaa gcaattgttg caagccatat
     9481 ttttcttctt tttcagctaa tttgattgcc cgtgtgataa agacattttg gaaatcaaaa
     9541 tcttttgcaa agttttccaa cattgttgag gctggtaaat atttgttttg accatgttcg
     9601 taaatttttc cataacgcat gacttcttct aaagtagacg gaacgatttc attttcaatc
     9661 actaactcat gagagatttt attgaaccat ctcgcttctt gatcagcata ttctgaaaaa
     9721 agggtactta attcttgcga agatgtaccc aaaacataca attgtgcttg gatcaatttc
     9781 atttcttcta atttaaaatt tgctaataca tgccccacca tggcgccagc agttggttta
     9841 tgatggtcga tatctgcttg ttttgtttct tgaacaaatt tttcttccac tatattcatt
     9901 gatttacgcc tcttttactt ttgctgattg gacttcataa cctaattttt ccacaactgc
     9961 agctaattgt tcagggtgga cttcatctgg atcaaagttt gctttgattt tgctcgcatt
    10021 aaacaaaact ttcgtattgc ttaccccagg ctgttgagaa accgcatgtt cgattttttg
    10081 catacagctt ggacaagcta gtgtatctaa ttgcataata atttttttca tcgtagttct
    10141 tccttccttt ttcttgatag tcttattcta gattacagct tccataaaaa aattgattct
    10201 tatcaagttt tacgattttt cttctttgct ttcgtgtcca aaatacaata aacgcatcgc
    10261 gttaaagata acgatcaaga tactagcttc gtgaacgaac atcccactag ccatgtaaat
    10321 aaacccggca aataagccgt tgagtaaaaa taagacgaca ccaatggcaa tcactacatt
    10381 ttcacgggta tttaaaacag ttttcttagc caagcggtag gcatgaacca acgattcaaa
    10441 attagattgc atcaagacaa cgtcactcgt ttcgatagca acatccgttc ctgatcccat
    10501 agcaatcccg atatcagccg cagccaaaga gggagaatca ttgattccat ctccaataaa
    10561 agcaacgcgc aatccttgtt cttggtattt tttcacaaat tgaacctttt cgtctggtaa
    10621 taattcagca tggacttcat caatgcctaa catatcagct acataatcag cagtcatctg
    10681 attatcacct gtcagcatca ctaactgttt tgctcctgct tgtttcaact tagctaattg
    10741 ttcagcgact tctggtcgaa tcacatctga cacacctatg atttgagtga tttttttgtc
    10801 gattgctaca ataatgaccg agctgcctgt ttgttgtaat tgatgtaaat cttgtacttg
    10861 ctttggtgtg agagaaagtt tgtgagcggc aatcaatttg gtgttgcccg cacaaactgt
    10921 atgaccatca atctcagctg tgattccttg tcccttgatt gtctgattat ctttgactgc
    10981 taattgttga aaatcaatgt ctttctcctg agcataatgg atcacggcgc gccccagagg
    11041 atgatcggat aacgtttcta atcgagccac taaagctaag ctgtcccctt catcctctgt
    11101 atagttcttg atagtggata cagcagtagt tcctttggtc aatgtaccag ttttatcaaa
    11161 gacaaaagta tcgattttgg aaaaagtatc cattgcttca ccgcctttga ctaagatgcc
    11221 ccgtttcgct ccattaccga ttcctgctac attcgaaaca ggcgcaccaa tgactaaagc
    11281 gccaggacaa ccaagaacca aaacagtgat tgctaaacga aaatcacgac taatcagacc
    11341 aacaaccaac gcaataagta aaacggttgg agtataatac tgagaaaatc gatcaatgaa
    11401 tttttccgca tgggatttgg tatcttgtgc ttcttctact aattcgataa ttttggcaaa
    11461 ggtcgtatca tctccgacct tagttgcttc aatcgtcacg tagccatctg ctaaaatcgt
    11521 tccagagaag acttcatctc cactgatttt gacgacttgt cgtgattcac cagtcactgc
    11581 ggcttcatcc gcataccctt tcccgtcaat gactcgtccg tccacaggaa tagctccacc
    11641 agtttttact aagacgatat cgccttcttc cacgtcatcg atttccactt cttcagtcgt
    11701 tccatcttct tggacaacac tagcagtaga tggggccatc tcggtcaaag ccttgattga
    11761 ctcccgcgtt ttggtcaatg ttttttgttc cagatagcta ccaaacaaaa acaagaatgt
    11821 aacgatagct gactccttgt attctccgat cataaaggca ccaagcacag caatagtaac
    11881 gagtaaatca attgagataa ctttgacttt cactgcctga tacgcatgga tcataatagg
    11941 tatgaccgca ataatagaag ctatggctag agcaatgtta tagaacaaga caaaaccagt
    12001 aaattttccg ataaaaccaa aaataatcaa cacagctgat aaggcggtga tatgatttcg
    12061 atgctcttgg atccactttt gtaatttcat ctttctccct cttttccttt cattcttacg
    12121 agactaagta tagatactta tccatcataa aaaattgacc actatcaagt ttagtcaaaa
    12181 aggcggtttt cttttggtag aatacactac aaaaaatagt tagcaagaag actcactaaa
    12241 agtgatcttg ccaactattg tcgtttctat cgaaattttc attgaatgat tttcctgtac
    12301 agcatacatc ggagatctat ttctttatct aagattttta tcttgctaat tttttccttt
    12361 agtctttcaa atccacgtcg ttcatagaat tgatacgtac agccactatc tgtaaaaagg
    12421 aatatttctt ttcctttctc tcttttttca aattctgtta gcaacttggt accgatgcct
    12481 tttgttttca aatcaggatt tgcagctaaa aaaatgatct ctccatctgg cgaatgcttc
    12541 tttaaatatg ctgcaagcat ttgttcattt gctgtctcgt aagctccttt actttcttta
    12601 gcaaatatat tttgtaaaaa atcgaatatc ttaacatata tttgctgtcc aaaagaacga
    12661 tgtttctttt tttctccttt tatctccgcc agcaaaacac ctgccaactc gtcctctgca
    12721 tacaaagcaa gaatttgagt tgctcgattc aactccatat acaaaaaata tcttccgtaa
    12781 agattcagca gaagttcatt ttccatatat ctctcaaagt tcattccttc aacagcaaaa
    12841 tagatagctt ttttgtaatc tttcttttct aagggtttaa aagttagttt catcatttcg
    12901 cctcctcaac ttcaatgatt gtgttatact tacattataa tagacacagt gtctactatc
    12961 tataatcaat catctataag tttgtcgatt aagcgacagc tttgctttga tatgtcggat
    13021 aaggagatag gtaaatgaaa aagacagata gacgcgtcaa aaaaacagaa aaagctttgg
    13081 ctgaaacact atccacatta ttagtaaata aaaagattca agcaatcact atacgcgaac
    13141 taacagaaac ggcagatgta catcgttcga ctttttatac acattataag gatatctatg
    13201 atctatatga tcaattagaa agcaactttt ttcgggatct aaatgaaatt ctctcttatg
    13261 atccagccca tagctatgag gaactataca ctcgcttgat tgactatgtt tacgcaaata
    13321 cttttttata tcgcttattt atcctcaacc atggtgatat ttcgtttcag aagaaagtta
    13381 aacaagttat tgaagaaaac tatctgaaca tatggctata tgaagacaag aaaacgatta
    13441 tcacagaaga aatgagattc tttactacgt accacgtcca aggttgtcta tccgttctca
    13501 atcggtgggt agaaagcaat tacaagtact ccaaaaaaga attattagaa ctcttacaga
    13561 aactgaatga tcacatagag aaaatcatgc cttgaataaa aacatactaa gcttagttaa
    13621 gaagaaccgt aaccttctcg tataacaact gaatcttatt tagccaaatt gtttactgaa
    13681 gattttttga gtaaaatttc tataattcct acttattgaa aagtagtttg tagtgctgct
    13741 tctccctctc taagtgatat atcttgttgt accaatcaaa ttcacctcat cattaataaa
    13801 ttacttttag gctaatttat tacaacttat aggttgaagc tgtaaccaat ttatagtcat
    13861 attatatttt tatcatccag tttattgaat tcttctcgtt tacctcaatt cattttcaag
    13921 gggaatcagc tcatcatcta acattttacg ccatgctcgc ttcatttctt gtgggtattt
    13981 aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac tgttgataat
    14041 tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag aagcatgtaa
    14101 ttttttctca gaaacaaacc actcattgga aaagacatga atcaaatcat cacttacttg
    14161 tgtaaaatag cgacgtttaa tgacaaagat atcttcattg ctatctagtg taccttcatt
    14221 tctttgtgct taaattcaaa gataattttc tcaataaaga tttttgattc cagtaagatt
    14281 cctttactcc ttttacaaca tagcgttgtc ctttgttttg aaacatcttc ttcccctctt
    14341 tgccattaaa aatagcccaa ttctctataa aga
//
LOCUS       DAZUKZ010000062        14344 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000062 DAZUKZ010000000
VERSION     DAZUKZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.063"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..83
                     /locus_tag="ACG8EF_001798"
     CDS             <1..83
                     /locus_tag="ACG8EF_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFS4947104.1"
                     /translation="DTIVLHQRKLDLLKEQKKGFLQKMFV"
     gene            110..445
                     /locus_tag="ACG8EF_001799"
     CDS             110..445
                     /locus_tag="ACG8EF_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947105.1"
                     /translation="MKFLNFLNYKYLNWVLYGITLFFIIYYFFTDDDIFLILAILFLV
                     SSNFSDNLIEVLTEPEIRSMTDEEQQIIKAMISENKKDVVVIKKIREYTNLGVLQAKK
                     VYEKKKKGL"
     gene            622..1572
                     /locus_tag="ACG8EF_001800"
     CDS             622..1572
                     /locus_tag="ACG8EF_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4947106.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2090..2500
                     /locus_tag="ACG8EF_001801"
                     /pseudo
     CDS             2090..2500
                     /locus_tag="ACG8EF_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017369153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            2943..3755
                     /locus_tag="ACG8EF_001802"
                     /pseudo
     CDS             2943..3755
                     /locus_tag="ACG8EF_001802"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            3750..3914
                     /locus_tag="ACG8EF_001803"
     CDS             3750..3914
                     /locus_tag="ACG8EF_001803"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947107.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            3854..3976
                     /locus_tag="ACG8EF_001804"
                     /pseudo
     CDS             3854..3976
                     /locus_tag="ACG8EF_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            complement(4538..5044)
                     /locus_tag="ACG8EF_001805"
     CDS             complement(4538..5044)
                     /locus_tag="ACG8EF_001805"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4947108.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RYWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRNSKILVPINILKSKISSLVSPLTEEL
                     QKIAMKVM"
     gene            complement(5049..5816)
                     /locus_tag="ACG8EF_001806"
     CDS             complement(5049..5816)
                     /locus_tag="ACG8EF_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4947109.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6306..6731
                     /gene="lacA"
                     /locus_tag="ACG8EF_001807"
     CDS             6306..6731
                     /gene="lacA"
                     /locus_tag="ACG8EF_001807"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFS4947110.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6748..7263
                     /gene="lacB"
                     /locus_tag="ACG8EF_001808"
     CDS             6748..7263
                     /gene="lacB"
                     /locus_tag="ACG8EF_001808"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFS4947111.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7274..8206
                     /gene="lacC"
                     /locus_tag="ACG8EF_001809"
     CDS             7274..8206
                     /gene="lacC"
                     /locus_tag="ACG8EF_001809"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFS4947112.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8209..9189
                     /gene="lacD"
                     /locus_tag="ACG8EF_001810"
     CDS             8209..9189
                     /gene="lacD"
                     /locus_tag="ACG8EF_001810"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS4947113.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9217..9534
                     /locus_tag="ACG8EF_001811"
     CDS             9217..9534
                     /locus_tag="ACG8EF_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4947114.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9534..11240
                     /locus_tag="ACG8EF_001812"
     CDS             9534..11240
                     /locus_tag="ACG8EF_001812"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS4947115.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11382..12788
                     /gene="lacG"
                     /locus_tag="ACG8EF_001813"
     CDS             11382..12788
                     /gene="lacG"
                     /locus_tag="ACG8EF_001813"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFS4947116.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12811..13083
                     /locus_tag="ACG8EF_001814"
     CDS             12811..13083
                     /locus_tag="ACG8EF_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HFS4947117.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13104..14003
                     /locus_tag="ACG8EF_001815"
     CDS             13104..14003
                     /locus_tag="ACG8EF_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS4947118.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4686 a         2363 c         2765 g         4530 t
ORIGIN      
        1 atgacactat cgttcttcat caacgtaagt tagatttgtt gaaagagcag aaaaaaggat
       61 ttctacaaaa aatgtttgtt tgatatttta aatagaaaag agagataaag tgaagttttt
      121 aaattttttg aactataaat atttaaactg ggtattatat ggtattacat tattttttat
      181 tatttattac ttttttaccg atgatgatat ttttttaatt ttagcaatac tttttcttgt
      241 ttcatctaat tttagtgata acttaattga agttcttaca gaaccagaaa ttcggtctat
      301 gacggatgaa gaacaacaga ttattaaagc tatgatttcg gaaaataaga aagacgtagt
      361 agttatcaaa aagattagag agtatacaaa tttaggtgtt ttacaagcca aaaaagtata
      421 tgaaaaaaaa aaaaagggtc tataattaca aattagataa tacattagaa gctggagaca
      481 agattgtcta gataatgtgt gagataaaat ttttggcagg ttatcataca taagacaaaa
      541 tgtttttaaa actgataacg tatttattcc acaacaatta cgcatttatt tctgagaaaa
      601 atataaagca ggaggattac aatgctgaaa acttattcct taacaaaaga tacattatta
      661 gtttcgtcag aatcaaatat caaaaattct tctgttgttt atgtttatgg tgacgaacga
      721 gaatatatcg aatcttctga acataaatat gactttaaaa taggcaatat actaacgttt
      781 gatgatcgag ttgcatatga cacgatgata gataagaacg atgaaaaaag tttgctcgta
      841 agttttttgt ttcccgaggt acatgaaggt gggcatctag ataccctgac gacatctgtt
      901 gttttcttgg tatttaaaaa taagttgata atttttacaa aacaaaaatt aaaattcatt
      961 cccgaactct tatccaatat ggttttacgt gatgtcaatc atttcgtgga ggaagtactt
     1021 ttaaagatca tgcaaaaaga tttctatatg atgcttaacg accttcgtgg agtcaaggaa
     1081 aaaattgatg atttagactc agaaatatca acgtccggtt cactcaaacc aacgtttggt
     1141 gatttattga ctcttcaaaa atacatgata gctttgtcaa caacttataa agctaaccat
     1201 aaagcgcttg atttcattaa aaaaaatttc acaactattg acgatattga ttttagtact
     1261 aaaaaaataa ttgatgattt atacgataca agtgatacaa tggatagaat cattttaagt
     1321 tacagccaat atttggacaa attggaaaat atgataaaca atatgatttc gtatcagcta
     1381 aacatgatta tgaaaaccct aaccgagata tcaattgttt tgactattcc agcaattatt
     1441 tttggctttt ggggaatcaa tgtccatgtc ccgtttgaaa agtcttctta tggtgtttta
     1501 gccgtattga ttatctctgc tatactaagt ctaatttgct ggcttttgat gcgacgcaaa
     1561 acatatttgt aaagtatttg tgatacccta atatgattta aagcagaatt tctttgttga
     1621 ctttttaaaa ggctgattcc tttcaataat ttaatagctt cagcggctgc tcagaagtca
     1681 ataaattgga ctatttttat tcagttactt aggcaacatt tgagatacga ttgcgattat
     1741 aaaaattgat ataccaacca atggctgctt ggaagactga catattagcc tcgatatcat
     1801 cgaataacta aatacagaaa aatacaataa atattatcaa actagggact atactagaaa
     1861 acatcagtac attccttaac tcagggtcta taattagaaa atcgcccctt tgaaaactga
     1921 ttaaaacagc ccccattatc tagtgactag ataatggggg ctgttttttt gttaagtgat
     1981 ataataaacc ataaaagaag tttagaaata cggtcctggt catcaataaa taaacaattt
     2041 aattaattat ttatttaatt atttaatttc agaaaggtat acatgaatca tggtgcaaca
     2101 aattgtccta cccatcaaag actccagcgt cttaaagatg gtacaagata cacttctcga
     2161 tagtttccgt gctggtcgcc gaaattacac cgtttttcaa gttggtaagg ccacgctact
     2221 acgtgtgagt gatgtcatga cattaaaaaa atcagatgtc tataatccag acggctctgt
     2281 caaaaatgca acctttattc atgataaaaa gaccggtaaa gcaaacacgt tatatttaaa
     2341 gcctgttcag caagacttgt tgcaatatca tgattggctg gcccaagaaa atatcaattc
     2401 tgagtggtta tttccctcga cagcccatta tgatcttcat atcactgaga agcagtttca
     2461 taaggtgatg gcgcatgttg gtgatctctt aggaatcaac ttggaagaca aagagaaaaa
     2521 attgcgagtc agaaaaccgt tttgagatat gaacagcatt tgccttcacg ccttagcgat
     2581 tttaaataat agccttgaaa gtgattatgg aaaagccctc tacgctcaat gcaaaattga
     2641 cgtagaaact atttttgatc gattgaaagg tgtatttggt atgtgaagaa cccatgtcag
     2701 aggcaagaac gttgcaccta ctgacattgg aatgatgctt atgagcatga atctaaccaa
     2761 attatccctt gaggctagaa gaaaagcgga agcttttcaa cacaaagcag ccaaaaataa
     2821 aaaacgcgac gaagcaatca catttatgat tattcatcgc gttttttata tttagagcta
     2881 gttatttgct agacccacta tttacagatg aaacgtttat cacctttggt tttgggcatt
     2941 tagtggaatt agcagaacca ggtcattatg acgaaaagtg gcaaaattgg aaacttgaat
     3001 ctttgccgat ttttcctgat cgatacgatt ttgaagttgc aaaagataag ggaaagcagt
     3061 ttaaaattgt tgcagaactt ctcaaaaagg caaatacaat tattgttgca acagatagcg
     3121 acagagaagg tgaaaatatc gcctggtcga ttatccataa tgcaaatgcc ttttcaaaag
     3181 ataaaacatt taaaagacta tggatcaata gcttagaaaa agatgtgatc cgaagcggtt
     3241 ttcaaaattt gcaaccagga atgaattact atccctttta tcaagaagcg caaacacgcc
     3301 aaattgccga ttggttgatc ggcatgaacg caagcccttt gtatacgtta aatttacaac
     3361 agaagggcgt acaaggtaca ttttcactag gacgtgttca aacgcccacc ttatacctta
     3421 tttttcagcg ccaggaagcc atagagaatt ttaaaaaaga accttttttc gaggtggaag
     3481 ctaagtaaaa caagggtcat ttaagggcgt tctaagcccc acacagcgtt ttaaaaccca
     3541 agaggagctt tttagctttt gtttcttcta aacaagctaa aataggcaat caagagggga
     3601 taattgctga tgttcaaacc aaagagaaga aaacgaatag tccgagttcg ttttctttaa
     3661 gtagtttgca atcaaaagtc aatcagcttt ataaagcgac agcgagccaa actttaaaag
     3721 ctatgcaagg actgtatgaa gcaaaattat tgagttatcc aagaacagat acaccattta
     3781 ttacagagaa cgaatttgct tatttaaaag cgaattttgg caaatatagc ggttttttag
     3841 gacttgatct tgaaatgttg ttatcattga taatgatacg ttcaaacaac agcacccaaa
     3901 ttttgacgaa ttagtgaaac tctatgaaaa agacgtagta aaacatgcaa catcctattc
     3961 taatcagctg gtaaagctta attaggttat ataagcttga aaataaaggg tttgtccaat
     4021 atggacaagc ctttttctta tgttttgcca cagatttgcc acacttattg tgtaagagta
     4081 tcaaggatag gtataatttg atactttgtt tttttagtga tgtgcaaata aatttctgtt
     4141 acacgactac ctctagaatg ccctacacgg tcttgaattg cttctagggg tattccttac
     4201 tctgctaaga aactaatatg cgtatgacgg aatatatggg tcgtaatatg tttattaaaa
     4261 ctaagtttat tatcaatatc aattcctgct cgttttggat taccttttcg atttaatttt
     4321 ccattttcag gtgagttgag gaaattgtta aggacactat aatgaagagg tgttccatta
     4381 tagtcgggtt tatgacgact tttggaagta aaaatccatc cattatcttt ataatcagga
     4441 ttgaatctca tatgttggtg atttaactca atttcctctt taagaatttc tatagccctt
     4501 ttagataatt ggacatttcg gtaactttgt aagtttttta cattaccttc atagctattt
     4561 tctgcagctc ctccgtgagt ggactgacta atgatgatat tttgcttttc aaaatattga
     4621 taggcaccaa tattttggag tttcgactct gtaagtcaac agggccatta gttagaatac
     4681 ctattgggat gcggttttta tgtagagcca gcagtagttg atgtatctca ggaatcatcg
     4741 taatctgatc gagttcatat tcatagtcag cttggaagtc tagagcttct gtcctagaaa
     4801 tactatcgat gccaaattct tccatagtct tctgacagcg aaaaattcta agctgctcaa
     4861 tactgatgag ttttttcgtg tatttaggaa aagcaacatc agaccaatat ctgaaacgtt
     4921 tgtaaatgtt atctatttgt tggatatcaa tcgttggaaa acatttctca acgctacgtt
     4981 taaaggggag ttgatggtca taaagagtgt catcaaggtc aaaaacaata tttttataag
     5041 tcataattct agtccgcttt taaaatagta gtgtactggc taaattcctc taagtgttga
     5101 gggctaatct ggttatctgt aatgatggta tcgagttgat ctagattata gaagttaaag
     5161 aaatcgtatc gatcgaattt agtactgtct actaataaga atttttctac agcattgttt
     5221 agggcaagtt gttgaatcac accttccttg tcactatatg tagcaataga attatgggta
     5281 acagcatttg cactaacaaa agctttggca aatctaattg cttttaaatt tgtcgaagcc
     5341 attgaaccta caaaagctcc agttatttct ctatattcac cgccaagaag caataaatca
     5401 atggtttcgc tatcatttag aatcaagaac acagggagac tatttgtaat gacacgaatt
     5461 ttataacctt tacgaccttt caattctaat gccagttgtt caagtgtagt tccaggtcca
     5521 ataaaaatag tatcgccatc tttgattaaa gagcaggctt ttttagcaat gtcaattttt
     5581 tcttttgtat ttagactttt cttctcgata tgtgtctttt caagtggctt tttagaggaa
     5641 agcaattgtg caccaccatg agtacgtgtt aaaagtccat cagcttctag agcatcaagg
     5701 tctctacggg ctgtcatatc ggaaatatct agttcatcta ttatttcttt tatggttatg
     5761 gtcccatcaa tctttaacat atctaaaatt ttttctaatc gtcgtttttt gttcatatga
     5821 agacccttct ttcataaagt aatttttttc caaagataat tctcttttaa ttgtatcata
     5881 aaagacaata ttttcaaggt aaaacaaaca atttcaaaca aaaacaaatg ttagatgatg
     5941 aaataagaac agaggattga cgtatattag cttaggtcag attttgtata agacgaaaat
     6001 aaagtaggac ctcttaatca gtaagttata gaaagtaaaa gacttttgta atacttgaat
     6061 agatatttca cgtccatttt gtgatggatt aaatgaacaa aaatgaacaa taatttaacg
     6121 gtgttatcta ttttttaaaa aaacaaataa aaaaaacaaa aaataaacaa aaaaagtgtt
     6181 gctttttgtt tgaatgcttg atattatata aacaaagaaa tgatgaaaac gttttttcga
     6241 acattttgca aaatgttttc tacttctacg tagcatttct ttctaaaatt taggaggtgg
     6301 tcaaaatggc tattgttgtt ggtgcagatc tcaaaggtac gagattaaaa gatgttgtga
     6361 aaaattttct tgtagaagaa ggttttgagg taattgatgt gactaaggac ggacaagatt
     6421 ttgttgatgt taccttggca gttgcctctg aagtaaataa agatgagcaa aacttaggta
     6481 ttgtaattga tgcttatgga gctggtccat tcatggtagc aactaaaatt aaaggtatgg
     6541 ttgcagcaga agtttcagat gaacgctcag cttatatgac acgtggacat aacaattcac
     6601 gaatgattac ggttggcgct gaaatcgtcg gtgatgagct tgctaaaaac atcgctaaag
     6661 gtttcgtcaa tggtaaatat gatggcggac gtcatcaaat ccgagtagat atgcttaaca
     6721 aaatgtgtta agaaaaggag ttaaggaatg agaatcgcaa ttggatgtga ccacattgtc
     6781 acagatgaaa aaatggcagt atcagaattc ttgaaatcaa aaggacatga ggtccttgat
     6841 ttcgggacat atgaccacgc acgtactcac taccctatct atggtaaaaa agtcggtgaa
     6901 gcagtagtaa gtggtcaagc agacttggga gtatgtatct gtggtacagg tgtcggcatt
     6961 aacaatgcag tcaataaagt tccaggtgtt cgctcagcgt tggttcgtga tatgacatca
     7021 gctatatatg caaaagaaga gttaaatgcg aatgttatcg gtttcggtgg aaagattact
     7081 ggtggtcttc ttatgaatga catcattgaa gctttcattg aagctgagta caaaccaaca
     7141 gaagaaaata aaaaattgat tgcaaaaatc gaacacgttg aaacacataa tgcacatcaa
     7201 gcagatgaag atttctttac agaattcctt gaaagatggg accgtggtga ctaccacgat
     7261 taaggtataa caaatgattc tgacagtcac actaaatcct tcagtagaca tctcttatcc
     7321 tctagaaaca ttgaaaattg atacagtgaa tagggtgaaa gatgttagta agacagctgg
     7381 tggaaaaggc ttaaatgtta ctagagtctt gtatgaatct ggtgataaag ttactgctac
     7441 aggtttctta ggtggtaaaa ttggtgaatt tattgaaagc gaattagaac aatctcctgt
     7501 tagtccagct ttttataaaa tctcaggaaa cactagaaac tgtattgcta tcttacacga
     7561 gggtaaccaa acagaaattt tggaacaagg accaacaatt tctcatgaag aagcagaagg
     7621 ctttcttgac cactatagca atcttattaa acaatcagaa gttgtgacaa tttcaggaag
     7681 tttaccttca ggacttccca acgattatta tgaaaaactt atccaacttg cttcggatga
     7741 aggagtagca gtcgttttgg attgttcagg tgcacctctt gaaacagttc taaaatcgtc
     7801 ggctaaacct actgccatca aacctaataa tgaggaactt tcacagttgc ttggaaaaga
     7861 agtaacaaaa gatattgaag aactcaaaga cgttcttaaa gagtcacttt ttagtggtat
     7921 cgaatggata gtggtctcat taggtcgaaa cggtgccttt gcaaaacatg gtgatgtttt
     7981 ctataaggta gatattcctg atatcccagt tgttaatcca gtcggatcag gggactcaac
     8041 ggtagctggt attgcatcag ctttaaatag taaaaaaagt gacgctgact tattaaaaca
     8101 tgcgatgaca ttgggtatgt taaatgctca agaaacaatg acagggcatg tgaatatgac
     8161 taattacgaa acactaaact cacaaattgg agtaaaagag gtataaaaat ggtacttaca
     8221 gaacagaaac gcaaatcttt ggaaaaaatt tcagataaaa acggtgttat ctcagctttg
     8281 gcatttgacc aacgtggtgc tttgaaacgt ttgatggcac agtatcaaga tacagaacca
     8341 actgtagctc aaatggaaga actaaaggtt ttggttgctg atgaacttac aaaatacgct
     8401 tcttcaatgt tgcttgaccc tgaatatgga cttccagcaa caaaagcatt ggataaagaa
     8461 gcaggtcttc ttcttgccta tgaaaaaaca ggttatgata catcaagtac aaaacgtttg
     8521 cctgactgct tggatgtttg gtctgcaaaa cgtatcaaag agcaaggcgc tgatgccgtt
     8581 aagttcttgc tttactatga tgtagacagc tcagatgaac ttaaccaaca aaaacaagct
     8641 tatattgaac gtgttggttc tgaatgtgtc gctgaagata ttcctttctt cttggaaatc
     8701 cttgcttatg atgaagaaat ctcagatgca ggttctgttg aatatgcaaa agtgaaacct
     8761 cgcaaagtca ttgaagcgat gaaagttttc tctgatccac gctttaacat tgatgttctt
     8821 aaagtagaag taccagttaa cgttaaatat gtcgaaggtt ttgctgatgg tgaagtagtt
     8881 tacagtaaag ctgaagctgc tgacttcttt aaagcacaag aagaagcgac taaccttcca
     8941 tacatctacc taagtgcagg tgtatctgct aaattgttcc aagaaacatt gcaatttgct
     9001 cacgactcag gtgccaagtt taatggtgtg ctttgtggac gtgctacttg ggcaggatct
     9061 gtagaacctt acatcaaaga aggtgaaaaa gcagcacgtg aatggttgcg tactactgga
     9121 ttcgaaaata ttgatgaact taacaaagtt cttgttaaaa cagctagtcc atggactgat
     9181 aaagtatagt aaaaacataa aacggaggat attgttatga acagagaaga gatgactctc
     9241 ttagggtttg aaattgttgc ttatgctgga gatgctcgct ctaaactttt agaagcgctt
     9301 aaagcggctg aaaatggtga tttcgctaag gcagatagtc ttgtagtaga agcaggaagc
     9361 tgtattgcag aggctcacaa ttctcagaca gctatgttgg ctcgagaagc ttctggggag
     9421 gaacttccat acagcgttac catgatgcat ggtcaggacc acttaatgac tactgtctta
     9481 ttaaaagatg tgattcatca cctcatcgaa ctttataaaa gaggagcaaa ataatgcata
     9541 aactcattga acttattgag aaagggaaac cgttctttga gaaaatctct cgaaacatct
     9601 atcttcgtgc tattcgtgat ggatttatcg ctggtatgcc agtcatcctt ttctcatcta
     9661 tctttatcct tattgcctat gtcccaaatg cttggggatt ccactggtca aaagacattg
     9721 agaccttctt gatgactcct tatagctatt cgatgggtat cctagcattc tttgttggtg
     9781 gtactaccgc taaagctttg acggactcaa tgaaccgtga cctacctgcg accaatcaga
     9841 ttaacttctt atctacgatg ctagcgtcta tggtagggtt ccttttgatg gcagctgagc
     9901 ctgcaaaaga aggaggcttc ttgacagcct tcacgggaac aaaaggtctt ttgacagcat
     9961 ttattgcagc ctttgtgaca gttaatgttt acaaggtctg tgtgaaaaat aatgttacta
    10021 ttcgtatgcc tgaagaggtt ccaccaaata tctctcaagt atttaaagac ttgattccat
    10081 tcactgtagc ggttgttctc ctttatggat ttgaactcat tgttaaggga actcttggtg
    10141 taaccgttgc agaatcaatc ggtacccttc ttgctcctct cttctcagct gcagatggtt
    10201 accttggaat tacacttatc tttggtgctt atgccttctt ctggtttgtt ggtatccacg
    10261 gtccttcaat tgtcgaacca gcaatcgctg ctatcactta tgccaatatc gatgccaact
    10321 tgcatcttat ccaagctgga caacatgcag acaaagttat cacttctggt actcaaatgt
    10381 ttattgttac catgggtgga acaggagcta cattgattgt tccattcttg ttcatgtgga
    10441 tttgtaaatc agaacgtaac cgtgccatcg gacgtgcctc agttgttcca acattctttg
    10501 gggttaatga gccaatctta tttggtgcac caatcgtatt gaatccgatt ttctttgtac
    10561 cgttcatttt cgctccgatt gtcaacgttt ggatctttaa attctttgtt gacacattga
    10621 acatgaactc attctctgcc aaccttccat gggttactcc tggtccgttg ggaattgtac
    10681 tcggtactaa cttccaagtt ctatcattta tcttagccgg actcttggta gttgttgata
    10741 ctatcattta ttacccattt gttaaggtat acgatgaaca aattcttgaa gaagaacgtt
    10801 ctggtaaaac aaacgatgct cttaaagaaa aagtagctgc aaacttcaat actgctaaag
    10861 cagatgccgt tcttggaaaa gcaggcgttg ctaaagaaga tgtagcagca aacaataata
    10921 tcacaaaaga aactaatgtc cttgttcttt gtgcaggtgg aggtacaagt ggcttgcttg
    10981 ccaatgcctt gaacaaagca gcagcagaat acaatgttcc agtcaaagcg gcagcgggta
    11041 gttacggtgc tcaccgtgaa atgttgccag agtttgactt ggttatcttg gctccacagg
    11101 tcgcttcaaa cttcgatgat atgaaggctg aaacggataa attgggcatt aaacttgcaa
    11161 aaactgaagg agctcaatat atcaaattga cacgtgatgg acaaggtgct cttgcatttg
    11221 ttcaacaaca gtttgattaa taaatttgta aaagttattt gagacgagat tattctttca
    11281 attccttata atattgttaa ccatatcatt gcatttcatt tccttggttt ccggaactca
    11341 gaatatcgtc tcaaatgctt tttttgaaag gatttatagt tatgactaaa acacttccta
    11401 aagattttat ttttggtggt gctacagctg cttatcaagc agaaggtgcg actcacacgg
    11461 atggtaaagg cccagttgca tgggataagt atcttgaaga caattattgg tacacagcag
    11521 agcctgcaag tgatttttac cataaatatc ctgttgattt agaattagct gaaaaatatg
    11581 gcgttaatgg tattcgtatt tcaattgcct ggtctcgtat tttcccaact ggctatggcg
    11641 aagtaaatga aaaaggtgtt gaattctacc ataatctttt tgccgagtgc cacaaacgtc
    11701 atgttgagcc attcgtgact ttgcaccact ttgacacacc agaagttctt cactcagatg
    11761 gagatttctt aaaccgcgaa aatatagaac actttgtaga ttatgccgct ttctgttttg
    11821 aagagttccc agaagtaaac tactggacaa ctttcaatga aattggccca attggagatg
    11881 gtcaatactt ggttggtaaa ttccctccag gcattcaata tgatcttgcc aaagtcttcc
    11941 aatcacacca caatatgatg gtttctcatg cacgtgctgt gaaattgtat aaagataaag
    12001 gttataaagg cgaaattggt gtagtacatg cgttgccaac caaataccct tatgatccag
    12061 aaaatccagc agatgttcgt gccgctgagt tagaagatat catccataac aaatttatct
    12121 tggatgcaac ctaccttgga cactattcag ataaaacgat ggaaggggtc aaccacatcc
    12181 tagctgaaaa tggtggagaa cttgatcttc gtgatgaaga cttccaagca cttgaagcag
    12241 ctaaagattt gaacgatttc cttggtatca actactacat gagtgattgg atgcaagcct
    12301 ttgatggtga gactgaaatc attcacaatg gtaagggtga aaaaggaagt tctaagtacc
    12361 agattaaagg tgttggtcgc cgaatcgcac cagattatgt gcctagaacg gattgggact
    12421 ggattattta tcctgaaggc ttgtatgacc aaatcatgcg agtgaaaaat gattatccaa
    12481 actacaagaa gatttacatc actgaaaacg gtcttggata caaagatgag tttgtagata
    12541 ataccgttta tgatgatggt cgtattgatt acgtgaagaa acacttggaa gttttatcag
    12601 acgcaattgc ggatggtgca aatgttaaag gttacttcat ctggtcactt atggatgtct
    12661 tctcatggtc aaacggatac gaaaaacgtt atggactatt ctatgtagac tttgacacgc
    12721 aagaacgcta tcctaagaaa tcagctcatt ggtataagaa agtagcagaa actcaagtga
    12781 tagagtaata ttacttgaaa atgtaaaaaa ttgttatccg atactttaga aaaagtacaa
    12841 cacttattta gaccaggctt tgaaaaggta tatattgtta gtttcgaaga ttgtcctatg
    12901 attcctgagc ttgaagctac cccgttgcta aaatgtggta aatggtatgt ttcaactgga
    12961 aaagagtgga tttgccattc tgatttagag ttatcagcat ttgaatggga atttttacaa
    13021 tcactcgatg tagaaatacg tgagactatt cacttcgagg taaattattt gcctttccaa
    13081 taataaaaag aaaggaatta gttgtgacta tcgaattaaa aaacgagtat cttactgttc
    13141 agtttaaaac acttggtggt caactgactt cgattaaaga caaggatggg atagagtatc
    13201 tctggcaagc tgatccaaat tattggaatg gacaggcgcc tattctattt cctatctgtg
    13261 gtagtttacg aaatgattgg gctatttata gacctcaaga aagacccttt tttacaggtc
    13321 ttattcgtag acatggtttt gttcgaaaag aagaatttat tctggaagaa gtcaatgata
    13381 atagcgtgac ctttagtatt aaaccaaatg ctgagatgct tgataattac ctatatcagt
    13441 ttgaactgag agttgtttat accttgaatg gtaaatcaat tagaaccgaa tttcaggtaa
    13501 cgaacttgga aatggaaaaa acaatgcctt actttattgg agcccatcca gcattcaata
    13561 gtcctttagt agaaggtgaa aactatgagg attattatat tgaatttagt gaggttgagt
    13621 cttgctcgat accaaagagt tttcctgaga caggattatt agatttgcaa gatcggacac
    13681 cttttttgga aaatcaaaaa attctcgatt tggactactc acttttttcc catgatgcca
    13741 ttacacttga ccagctcaag tctcgtagcg taacgcttcg gtcaaggaag tctggtaaag
    13801 gtcttcgagt agattttgat gattttccga acttgattct gtggtctaca actaataaaa
    13861 gtccattcat tgctttggaa ccttggagtg gtctctccac ttcacttgaa gaaggtaatt
    13921 tcttagaaga taaaagacag gtaacgaaaa tttcacctca agaaacttct agaaaaatct
    13981 atgatattac tatccttaat taaaatttgt tattatttga gtcttcaata agatttgaat
    14041 actcttacct tgtgatgata gattggtctt ctccgtgcca gtcttacatc gcaaggtttt
    14101 taagtttttt gtgggtctat gtcaactgta gtgggtgacg aaaagctaat atctaaagac
    14161 agtctggtag taaaaaataa gattatttac tacttcttga tttaaaataa tagtggagcc
    14221 attaatttgt ggcaaattta atttaatatt ttgcctcaaa ttagccacac tttttataga
    14281 actaagagga aaaatgaaat tttcttccta ttaatatagt tgtttgaaat cttaaatata
    14341 gcgt
//
LOCUS       DAZUKZ010000063        14328 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000063 DAZUKZ010000000
VERSION     DAZUKZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.064"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..1688)
                     /locus_tag="ACG8EF_001816"
                     /pseudo
     CDS             complement(53..1688)
                     /locus_tag="ACG8EF_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2243..2806)
                     /locus_tag="ACG8EF_001817"
     CDS             complement(2243..2806)
                     /locus_tag="ACG8EF_001817"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS4947119.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(2821..3864)
                     /locus_tag="ACG8EF_001818"
     CDS             complement(2821..3864)
                     /locus_tag="ACG8EF_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947120.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(3997..4401)
                     /locus_tag="ACG8EF_001819"
     CDS             complement(3997..4401)
                     /locus_tag="ACG8EF_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS4947121.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(4634..5269)
                     /locus_tag="ACG8EF_001820"
     CDS             complement(4634..5269)
                     /locus_tag="ACG8EF_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS4947122.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(5535..6587)
                     /locus_tag="ACG8EF_001821"
     CDS             complement(5535..6587)
                     /locus_tag="ACG8EF_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4947123.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(6600..7538)
                     /locus_tag="ACG8EF_001822"
     CDS             complement(6600..7538)
                     /locus_tag="ACG8EF_001822"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4947124.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(7989..8639)
                     /locus_tag="ACG8EF_001823"
     CDS             complement(7989..8639)
                     /locus_tag="ACG8EF_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947125.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(8739..9248)
                     /locus_tag="ACG8EF_001824"
     CDS             complement(8739..9248)
                     /locus_tag="ACG8EF_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFS4947126.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(9449..10318)
                     /gene="scrK"
                     /locus_tag="ACG8EF_001825"
     CDS             complement(9449..10318)
                     /gene="scrK"
                     /locus_tag="ACG8EF_001825"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS4947127.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(10338..11459)
                     /locus_tag="ACG8EF_001826"
     CDS             complement(10338..11459)
                     /locus_tag="ACG8EF_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS4947128.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(11461..11856)
                     /locus_tag="ACG8EF_001827"
     CDS             complement(11461..11856)
                     /locus_tag="ACG8EF_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4947129.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(11875..12672)
                     /locus_tag="ACG8EF_001828"
     CDS             complement(11875..12672)
                     /locus_tag="ACG8EF_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4947130.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(12674..13462)
                     /locus_tag="ACG8EF_001829"
     CDS             complement(12674..13462)
                     /locus_tag="ACG8EF_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4947131.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(13476..13952)
                     /locus_tag="ACG8EF_001830"
     CDS             complement(13476..13952)
                     /locus_tag="ACG8EF_001830"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4947132.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(14024..>14328)
                     /locus_tag="ACG8EF_001831"
     CDS             complement(14024..>14328)
                     /locus_tag="ACG8EF_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4947133.1"
                     /translation="LNPTKLLERVSDAIYILQNDLGISFDNNTCFGLYVHISCLIERL
                     VKQNTLEDEIYFNETSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         4543 a         2974 c         2166 g         4645 t
ORIGIN      
        1 tattacttac tttttcttat tctctagtaa gctaatattg aaaaaaatgt aatcagatca
       61 ttatttttat atgttgtaac ttcctgataa gaaaactaat taaaagaaag aggtaactac
      121 ataaactaat taaaaaagct aacttcatag ttggaggata aatggaagaa acttcaatga
      181 ttgtttctcc ttgtgttcct tcaatcgaca ctagtcctat tatcaatctc tcttttttgt
      241 ccatttactt ttgcgactgt tccccaataa tcaaatacag gaagcgttat tttttgtcct
      301 ttagattcat gtactgtata gactattttc gaatccatca cttgataagg catagtatgc
      361 cattccttgc caattcttac ttcatgattc tgcaaatgtt ctttgtattg taatgcttcc
      421 gtgggtgcat agtcataaag tcctccggga aggagtgtcg tgtaacgaat aagattttct
      481 tgattcatta ccaagtgatc tctctgagtg acaactttat gttcatagtt tgcttttgtt
      541 gcagcagata cagccaacag taataaaaca attgccgtta tattaactac ttttctttgc
      601 ttagctgatg tgctcttcat gaaaacgaac gataaaatca acgagccaaa cagtacttga
      661 aagcctaaga ttcgccaagg aaattgaatc atttgaatgg gtgtattttg taataaagac
      721 catggaaaaa atttggtgga taagagccaa gagactaccg cactaaggta aatcactttt
      781 tctttgcgat taaatccttt aaaaaagaag agtggacaga tcaaagaaat aataccaatg
      841 attcctaccg taaaggttgt caggtcatta ttcatcgcac caatcaaact atccgagaca
      901 tttaatgctt ggtgttgtaa ggtcgtttta tctggctcat taatcgattg aaccatgatt
      961 tgtcggaaca tcggatacca aaagtatgct gttaaaagaa tcgtcattcc tactgccttt
     1021 gacaactcca caatcaaccg gatgctttct ttttttcttg tttcacttac tacaaagccg
     1081 cctaagacta gtaagccaac ggctattaca ctcataaaag tggaaagaac atgtgtataa
     1141 atgagtaagc tcatactgac tgctaatgga attgcacatg gctttttctc ataaatcagt
     1201 gcgtaagcat aatacataac tatcggtata accgtaagtg caatcgcttc agcaatagca
     1261 gaacgatgat aaatatcaat cgtacggtac ccataaaatg tatataaaca gctaaatata
     1321 aaagccgcat cgattctttt taaaaatttt agtccataat gaaacatcag catgatagta
     1381 caaacattca atagccaaac atataagata taactaacga ttaaattatt acttatccaa
     1441 taaaaaatga ctgccggaaa aaaggtcaag tagggataaa aataattcac tccgctccca
     1501 taatgattaa acgtcgtaaa attgattgga ccagaaaaaa ttgaagataa ccctttgact
     1561 ctagcaaaat ggaaggcaat atcccccgat gtagaaatag tatgaacatt tctaaaataa
     1621 ttgattgaat aaagtccagc aataattgct aggagaatgg acaatattat ttttttcttt
     1681 acttgcatta cttaattata tactcctttc atggattgct ttatctagta taacttaaat
     1741 tttaaataaa tcgttattta tttttactct cacttaaaaa agcactaaaa ttataagaga
     1801 gggaggtaat ggtctgaaca ccaagaattg ttccaacatc gaattgctta actaatgagt
     1861 tcaaagctta attatgtggc tcttatattt accgagcgtg gattgcgatc tctttagcga
     1921 aacaaacatc tttcataaaa aaaacagcca tatccgcttt cttggatatg actatttact
     1981 actactttac tatctttcat tcttcttgtc tcacttatga taaggcgctg cgtatcatga
     2041 gtgaatgata ttaggaaaac ttcataaaat aaatctttta tacatttcca cttctagatg
     2101 gtcattcatg ttagaagaat caaactattt ttttactgtc gtaataatat taaaacacta
     2161 gaacaaatca attaaattcc ataaaatata aataatagac ctagcatcat ctggtataaa
     2221 atggatgcta ggtccgttat atttactcaa aattgattct acgaatagat cttcttatca
     2281 tttctatttt agtagtcatg cgttgttgtt cttctaaagg tggtaatggt ataagtagtt
     2341 tagataaatt tgtaccattt acatttggtt gcccagttcc gtaagaaatt ttttctaata
     2401 acttccaata taatggcgag tctaagaagc agtctacgta ttctgataat aatttctcaa
     2461 ctaacctaat tcgaatcaag taagaagcaa aaacagattc ctcacttatt tccttaacca
     2521 agaaactttt tcccatagtt cctcctgtcc ttgctatcaa aatgtcattt tcttccaatc
     2581 ttaaatcaac tagtttagaa ttactaatat cacagtaagg cacactggac caatttacac
     2641 gtccctcttg tatatctgtg attctcaaaa acttaacatt gccttgtttt ttcgccgaat
     2701 ctgtatagcc atattgaatc ttagtagaaa tacttcctaa ataaacccat agtcgaacat
     2761 cgctagatga tttttcttgt cgttctttat gaacaacagt gatcatcttg agttgtccga
     2821 ctatttttta gttgcagcaa tctttttggg acgcttattt gcatgaagca acgttgctaa
     2881 gtctagtaat aaaacattgg ctaacacagg attggctttc tcgcgatacg ctgcggcgcc
     2941 actttcaaac aatactgcta gacgatcttc tacaacaacg atttgttcca aatacggtgg
     3001 aaatttgatt cgttttagga tccggctagg agagtcccgc tcggacacta agagttccga
     3061 ggtataagga ccataagaac gactggctaa gagataatgg ccataaaaag ccacaccttg
     3121 aatcttatca gatgtttttc tgctcattgt cacggttggt tggattttgt ctttgcggcg
     3181 aattgctgtt ttcatttcag ctatcgtcgg caatagatag aaagtcaact gtccttcacc
     3241 ttttagtgtg aagttgccag ataccagttg atcattgttt tgagtcaaat acgatgcttg
     3301 tggaatctct gagagatcag ttgtcagcca ataagcgatt ggctgtcccg tctcttcaga
     3361 tgtatctgct aagatatcga tcaaacgtaa agcggctact cgtccagtag cagatcctgt
     3421 gattgtcaac cataaaagtt cccgttttgt atcatatact aagcccccta catgtggccg
     3481 tttaggtaaa atcagcagtt tgatcggctg tcctgtttct ctatccagta catgaataat
     3541 agaatgatgc tgatggtcat agcagtaggc agaaataaac acaaaatctt gagaaaaagc
     3601 caacccttgc ggcgtcatcc attgacaaac ctcattttcg ccggtttccg gcaccaccat
     3661 tcttgtttct gttaatccag gaatgttata ttgttttgct tcagcctttt cccaagctgc
     3721 ttgaacaata ggataccgtt ttaacttttt ttcaatatcc tgatgtatcc gttgctgctt
     3781 ccaatgctta aactttagga catagtaaac aagaaggcca atgaccagca acactaaaat
     3841 cattattgct acccgctgtg tcattttttt gcctccttgt tctattcgat caatcgtcca
     3901 tataccgcta ttaattaccg taactacatg tttcttttac tagcgtttct ttataggatc
     3961 tttcataatc tcatttatag taacattaag aatcccttat tctactatag tcacttccac
     4021 atcaatattg cctcttgttg ctttagagta tgggcaaaat gcgtgggctt cttctgcgat
     4081 tttctctgct tggtccttct caagtcctgg caaagaaact tctaaaacga ccccgatttg
     4141 gtagccccca tcttctaatt gaaataaaga tacccgtgct ttgacttcag aatcagactc
     4201 aagattattt tcttccagca tgtgttgaac cgcgccgttg aagcaagatg cgtatccagc
     4261 cgcaaaaagt tgttccggat ttgtcccttc actatggatt cctgggcggt cgatcttcat
     4321 gtgcatactt ccgtttggtg cctcaacttc gccatcacgg cctccacgat tgatcattgc
     4381 tgtttcgtac atttttttca ttgagatcat cctttctttc gtcgttctat attaagtgta
     4441 acgagtattt tttaatctga caaaggtaat gcttgcattc ggcgacatca gttagacaac
     4501 atcgatgatc agtgatagaa gcagtttatc tatacaaaaa aagaagggtt ctatgatatt
     4561 tggtgtcctt actcgccttg agtaacaaca ccaaatatca tagaaccact taatcagctt
     4621 cacactgctt tttctacttt cgtagttgaa aaaaacttaa acaaatcatc ggcaaatagt
     4681 taaagaaaaa acttaatcgt tgaaagaagc ccaattgctt aaagaaggca aactgactaa
     4741 tcgacggaat ccgtgctaat ccgtaaagac ctgcaattag taaactaata actgttaaaa
     4801 caagataaaa atggctaagc gtaccttgac cactttgacg ataaagcaga acaagtaaca
     4861 atgggaaaag tagaaagcct gcatagccta gcccagaacc tatattatgg atccaagtcg
     4921 atagattaca agagctttca ttcgtattga tactgaataa gccagtaaaa atacaatcac
     4981 ctatcccata taaaccaact gttgcaacaa caagtattgc aagtgttttt gatgttccaa
     5041 caaataggtg atacaacgca ggcaacgaaa gaacaaaaaa aagtccagat acaactgacc
     5101 agaccaagaa agcacgtctg actggactat ctacgtcacc aaacacacta attacggtag
     5161 tcatttgatt cagtttggga taaaaaaggc caaggatata cggtgtcgat aaatcactca
     5221 tcacacctaa taataaaaaa taaaaaccat atttttgcaa aaaaatcatt ttcttcccct
     5281 actttttagt gattattcga tacatccaac aatcaaattt tttctcattt taacacatat
     5341 ttttattcta gttgtctttt gaacaatcaa taacataaag gtgagtttct tctacatttt
     5401 aaaaaatcga atggcactga agagttcagt tgtcagcatc aatctcctac tcaaaaaggc
     5461 aaaaaaacgt aagtcagatg ttgttgacat ctcacttacg catccattga ttgaaaattt
     5521 cttagtaaaa tctactagcc taaaaatttg aagacttctt ctcgatcata cagtttaaat
     5581 actttctcgc ataaaaattg agtaattagt gccgcaaaaa cagggatgac taaccaacaa
     5641 agaacaacaa gaaagccatt tagccctgca tctaaagaag ccaacggacc aacaagtccg
     5701 accacgccga aaccagcaga agcaggcgta cctgaaatag aaaatagagc gacaggaata
     5761 gctgtaatca ctgcactaac taaacaagga ataagaatca caggttttct aaatagatta
     5821 ggcatcatca tcttcatagc gcctaaagca atcgcaattg tcacacctga cttattaacc
     5881 ttccaagagt tcactactaa aacaaaagtg gttgcagcta cgcccatcgc agccgcacca
     5941 gcagaaacac catctagttg gattgccatt ccgattgcta ctgttgaaat aggagagata
     6001 atcaaaaagg caaaggaaca accaatcaag atagacatta acagcggttg taaggtagtg
     6061 aagttattaa tcaatgatcc aattgcagaa gtaattgctg ttacaaaagg gaataaataa
     6121 aaaccaagta aacctgcacc tacacctaca acaatcggtg ttaacacaat cgacactgac
     6181 ccaaaactat ttccaatcag taaaatcagc ccgacagcaa tcgatgctgt cagcatcgta
     6241 ttaataatat cgcctgtacc agcaccaacg taaacacccg tttcacccaa atcaggtgtg
     6301 aatttgacta caccagaacc aacaaaagaa gctccggcta caaccatcat tctcatcgga
     6361 tcaaatccaa attgtaaggc aatcaaacca ccaatgatca aaggtgttgc tagttgaaaa
     6421 ataactgcta actgtgcgat cgttaccgca aagggatacg cactaaaata ttttaaaatg
     6481 gcacctaata ctgcattagg aatcaatcca ataatgattc ctgttgctgt accagcgagg
     6541 actttattca gaaaaatttt cggtgatatt ttttgtgaag tttccattca atctctcctt
     6601 tattttacgt tcaaactatc ttcttttgca tggacaagct cagtcaagaa atcacaataa
     6661 ggcgtcgcaa caccatattt cttccctttt ctagaaattg cgccattgat ataatcgatt
     6721 tctgttaatc gatggttctt aatcaagtct tgatacattg aaggataatg taacccaatt
     6781 gtttccgggt caaaacaaga ttcacaatgt gcaatgactt ctgggacatc tagttcaatc
     6841 ttctctactg ctgctacttt ggcaaactca ttgacaatcg tcgccaccat tttatgagca
     6901 gtcgatgttt ttcctagctc agccatatta acgtctaaga ttgtgcatag tccgttcatt
     6961 gttccgttca cacatgcttt gcgataaata gaatagtgaa tattgtcaga aaaatgagca
     7021 tttaatcctg attcagacaa tttgtctgct aatttttttg cagctgcttc ttttccatca
     7081 cctaaatttt gtaattctac tgaaccactt ccaaataatt tgacctgacc aggaccttca
     7141 agacctgccg tccacatggt atttccaata tagatatttt ccattggtac aaatttctca
     7201 ataatatctt catgcccgat accatttaat agacataaga cctctgtatc ttttttgatt
     7261 aatgattgga tatcctgcag cattttttcc agctgcatcg ctttggtaaa tagaataatc
     7321 agatcaactt gatcttcttt ttctacttcg gattgaagaa cgattggtag ttttgcttct
     7381 acctctttac cattaaaatt tgcttgtaat ccatgttctt tgatttgttg gacatgttct
     7441 gcccagccat cgattaataa tacttcgttc ccactttggt gaagcatcaa cccaaagcga
     7501 cttcccattg cgccagcgcc tgcaattgct attttcattg attcttcctc ctttttcttt
     7561 ctattatact catttgagaa tcttttcaca acctcgtttt gttattcttc tcttatttat
     7621 catgtttata tccaccattc aagttatttt atcagcaaat caataaaaac tggaatagaa
     7681 tcccaatttc atcgtaagag aaactagact agcaataaga aaatagaaac attcatagaa
     7741 aaaatctcac tcctctgatt ataaaagagg gggcattaac ctcgtgtata ctaaaacctt
     7801 ccattcattc gatggatgat tttctttttg tgaaaaaata ttcattgtag tacgatgttg
     7861 cgcaacacag acggatggta ttgcttatta tttattaaaa tcgaacaata cacttattta
     7921 aaaacttctt tcccagattt tcttccattt ttacagttat aactagtatt atcatgaaaa
     7981 atagcctttt attgtctatg ggaataataa tttaaaattg gtactttttt attaattagt
     8041 tcatcgccca ataatctctc cagtttctta gcatctcctg actcaaccgg aattatccaa
     8101 gcccagtcta aataattttt taaaatctca tcatattttg caaatatttc attgccatat
     8161 tccgtcctta aaattatatt ttcagtgcca gacaagtgat ttatattgag ttgataaaaa
     8221 agatctccat ctaaaaaaat atcattagaa gaagatttcc ccacagtaat gaccattcca
     8281 tcagctctat gtgttacata caaataattg tatgacctca tttcacctaa tgaatcaatt
     8341 atttcttgcc ctttagctct aatatattga tctctattac ctatctcact cttatttaac
     8401 aaaagttttt tcatgtcaaa tttaatcggc tgttttattt ttgtaaattc gcttctacta
     8461 ctaatccaat ccgagattaa tttttcaaca tgtcccagag taaactctga tttattcgct
     8521 aaatactttt tttgttgtat taaagacatc tttccccgaa gctcaaaatt gagcctacat
     8581 cgattctcta tctcttcaaa aagcatctta tcttcatcat tcttatattc atcattcata
     8641 aaaccaatcc agctcctcga ttaagataat attttaatct catacaaaaa ctagctctca
     8701 tcaaaacaac cattgttttg atttctcatg aaatttcttt atacttttct ccgattataa
     8761 attcctaacc ccttgagaaa attacgcata tatttatctc cgcaaggctt aaaatttcgt
     8821 tggccttctt ttctcaaaaa tgccccgatt tcgccttttg atggatagat ttctgcctca
     8881 gcaaaaacgt ctaaaatatc atcagtggtc atagacaagg cgatttttaa tttttttagc
     8941 aacacattgt tgatatattt gatttccttg cttttttgcg gcgtgtctac tatcggctta
     9001 tctttttgcg gaccacgcgc caagagaatc aggccattta ggaatgcgtc aaaaacttgg
     9061 ttgttgatgg ttttttcata cacattattt tctgcattat ctttgttctt atcctgtact
     9121 ttggtcagta ccgcctgagc ttcttcttta gtcagtggta caccgcctag ctcaaaagct
     9181 ttaatcaaat ccgtatcttt caaatcaatc gcataacgca atcgcaataa cctatcattg
     9241 ttattcattt gttcctccga agtatttagt ctttactttc ttacgtactt tttgtcttgc
     9301 taagattttt aggatcaatg ataaaaaccg catcacttat gataaggcgc tgggtatcat
     9361 gagtgaatga tattgctgaa tacaaatagt ccgaccacat taagttgatc ggacttcata
     9421 agcattttat tgactaatac ttttttgctt aagaattgat tctaacatct tttgctaaag
     9481 ttaagcatcc tatagttcct gcattatctg ctaacttagg tgtgacaatg tattcttcga
     9541 tatttggcgt atcgacatag ccgttaatta gttctaaaaa acgtctttgt actttctttt
     9601 tcagatgttc ttgttgcatg acaccaccac caaaaattat aatatctggt gaaagcatca
     9661 gggtcgtatt acaggcacat tgtgcaatat aatccgcctc gatttcccag aacggatctt
     9721 ctatagaaag atcttgtccc ttggttcccg tacgcatctc gatagcgggt ccagatgcca
     9781 acccttcgag acaatcatga tgaaaaggac aagtaccaga gaaagtatct cctgaatgtc
     9841 tcttaactaa cgtatgcccc atttctggat ggctaaaccc ttcaacaaaa atcccatctt
     9901 gtatagctcc tccaccaatt ccagttccaa cagtgtaata aacaacactg gataaacctt
     9961 ttccatgacc tgaaacatac tccccgtaac aagcagcatt cacatcggtt gtccaggcaa
    10021 ttggaatttc gaaatgctta ttcaacgttc caacaaaatc aaaatttttc caggcagttt
    10081 ttggagtaga ggtgatatgt ccataggtct ttgattcacg atggatatca atcggtccaa
    10141 atgaccctac tccaatgctt tctaattgtt ctttatagtg attaaagaaa tcaattacca
    10201 aactcattgt ttcattcggt gtagttgtag gaaaactcac tctttctaaa acctttaaat
    10261 catcattgcc aatggcacaa acaaatttag tcccacctgc ttctattgag ccatacattt
    10321 cttactccac cgtcctttta tctaggaatt cgttcttccc tgtctaatag tttttcgaaa
    10381 aactcatgtg gttccttttg gtaccaatag gcaacagaag aaatatcgtc ttgacgctca
    10441 taaagcttga tgtcatcatt tcctagggtt tggaaagtaa cacgtaaatt ttctttgaaa
    10501 ctaatggggt cgggtaaatg ccagcggtac aacccatgca tcggtaaagc atcatcgcca
    10561 aaaccatgaa ctggatttgg attaggtttt ccagcactaa aataatcccg ggtgtgatct
    10621 cttttagatt gaaaaggata gcccataaat agcgtattaa aattttgagc atatgccctt
    10681 tctccaaagt tctttttatg gaaagcccaa gcgccgccaa aataatcttc tgctcctgta
    10741 gatgttattg tagggaaatc ttcatctcca tctatataaa acttaaattc tccttccccc
    10801 caccagtatc tttccaatgc gcacaaagca aagtaggttc ctacataata cccagtgcct
    10861 ttgattccat caataagggt gtaatctttt ccttttgtcg tgatgggttc acggttccat
    10921 ttagcatgaa aatacatcgc atcatccggt acttcagtca atgcataatt aaatgtataa
    10981 aaaatgtgct taataaactc aggatgttca ttcgttaaag taatcttagc tttttttcga
    11041 aatggcatct caaaatagca attcatccct gatgttggat tcactacgat tggtaaggaa
    11101 ttcacttcgc atcttgtccc aaacccatta caaaaaaaat ctcctaatgg actttctaca
    11161 gaaggagtgg attcatcatc ccaataaata cgcaaaatta aatcacgtaa gacgaaactc
    11221 ccagcgattg ttttgtctgt cactgtcatc caaaaatgac gtaaaattcc agggccttcc
    11281 atttctgcta aagtaattgt ctctccagca tttatcggaa cagaaggtga cccttttctc
    11341 ccaattccta gatgactcgc tgtcattgcc gctttccctt tttcaccatt tttattttct
    11401 ggtgaaatag ttctactttc ctctcccttg tggagccata tattatttaa acaattcatt
    11461 ttattcgtcc tctatccttt cttcactttt ttgattattc atatagatga tttgttgttg
    11521 tgcctccgca ataattgctc caatatctaa attatcattt cctaactgca aagcgattga
    11581 cattgccaag ggaagattca ttcctgcaat tacgtgcact ctgtcagaaa tataggtaaa
    11641 aaacttttga tttacacttc ctccagccat gtcagttaaa acaattattt cgtctgtagt
    11701 atccactaac tctttaattt gttcatctat atcatcaatt tcttcgacat atgcacataa
    11761 gctactaatt tgattatgca tacctgttaa aaactctagt gtctctttaa aaccctcagc
    11821 tagtattgaa tggctagcaa tgatcaatct tctcataatt caaatccttt tcctttacgc
    11881 taatattcca aatgcagcca taaccatcgc aaaaacaata acgagtagaa tcaaaatggt
    11941 catactgatt tttttcttac tgagtagata atatgctgca gcagtaataa ttactggaat
    12001 taatgttggt aaaattttgt ccaacattgg ttggattttc atactaactt caccaaaagt
    12061 gaatttaagt ggcgttgaaa cactgataac tgaagcaatt aatgatccaa ctactgttag
    12121 ccccaatact gaagcagctt cagttaaata attaatcttt tcaccgtatt cagtaacaat
    12181 cttcattccc tgcttataac caaattcaaa gcctctcatt cggaaaaaga agattaccat
    12241 gattaaaatg aaccataaaa agattcctac aggattattt tctaaagcca tataagccgc
    12301 aattgatccc atgatggttg ggatcatcgt catcaaaaga gagtccccaa taccagcaat
    12361 tggacccatc gtaccaactt taaagtcatt aatcgcatct tttgcttcta atccttgctt
    12421 ttcctccatt gcaacacaag cacctaaaat aatggctgcc agccaaggct gtgtattgta
    12481 atactgaaag tgattattta aagcttcgat gtattcatca tcatctttgt ataattttct
    12541 taaaataggt gacaatgcgt aagtcacaga tgcaccttgc tgtgtttgat aattaaaggt
    12601 acaaactgac atcaaccaac gatagttcgc tttcctaaga tcctttttcg ttacttgata
    12661 tttttcgctc attttattca tcctccccaa taaacccaac atcagaagct cccgcagaag
    12721 cagtgatttg tgcatctttt tcagctctat gatacacatt aaatgctaaa gcgaatccta
    12781 tgatcgccgc tccaataata ggtaccttca aaaaagcggc taagaaaaat ccaacaagta
    12841 aataactaat gtatcgacct gtaggcataa agcgtaacaa catcgcaata ccaacagcag
    12901 gcaacatttt tccggctaat gttagcccat ccataaacca attaggtaaa acatccaaaa
    12961 cgtaattaac agcagactgt cctaaagtaa cggcaataaa tactggaatt gctgcagata
    13021 aaccaaagaa aattggcccc aaccacaaaa cactattcat tttcttaaat tctcgtttat
    13081 tcgcatattc ctgtgatttg cgaacaacaa aaccatttaa gattttgaca attacatcta
    13141 gttgcacccc taacatacct acaggtagtc ctattgctaa tccggcttct actccaagct
    13201 ggctaccaaa tgccgttgca ataattgcgc acgttccata gtccggtaca gatgagccac
    13261 caaaaccggc aactcctaat gacatcaatt gaactgtacc gccaactatc aaacctgttt
    13321 ttatatcacc catcaaaatt cctgtgaata atccccaaaa aactgcgtaa tttacaaaga
    13381 tcatcggaat cccataataa tcaacatgtt taataaatgc taatatagtc agcataataa
    13441 tttgaaaaat agtaaaatgc attatcctta ctcctttatc ctaattttga ttgaatttcc
    13501 tgtgccttat ccgccggaac aaattgtgta acaatttcta caccagaatc caacaatttt
    13561 ttatacgtgc tgatattttc atctgatgca taggctctag aagatagctg taatgcatta
    13621 tctttaacaa cagtaccgcc aacaattagt cggtcaattc taataccgtt atctactaaa
    13681 tgaaggattg tttccggctc tttcacgatg acaaatactt tttcctgatc gtatttcccc
    13741 tttttaaaat tcgcaatcgc tgtttcttca gtgataattg atacagccat tccagcaggt
    13801 ctagctaaac gcatacttga tttttggatg tcgttatttg cgacagaatc atcgatcacc
    13861 atcactctgt ttgctttaga cattggtgcc cattgcgtga caataatccc atgcaataaa
    13921 cggttatcaa ctctcgacat aacaatactc ataaatactt cctcctttat ctttcatttt
    13981 cttacaagca attaccatgc caaaacaaaa agaacttgat aaatcaagct ctttttacat
    14041 aatcataaac gtatttaacc tcatgaatcg ggatgtcgac actataataa tgttcaacga
    14101 cactgaaaga ttgcttaaaa tgtgtctgaa atttttgaaa ttcctcactc gtttcattga
    14161 aataaatttc atcttccaaa gtattttgtt ttactaatcg ttcaattaag caactaatgt
    14221 gaacatataa tccgaaacac gtattattat caaatgaaat tcctagatca ttttgtaaga
    14281 tgtatatagc atctgaaacc cgttctaata atttagtcgg atttaata
//
LOCUS       DAZUKZ010000064        13954 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000064 DAZUKZ010000000
VERSION     DAZUKZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.065"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..293
                     /locus_tag="ACG8EF_001832"
     CDS             99..293
                     /locus_tag="ACG8EF_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947134.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="ACG8EF_001833"
     CDS             562..861
                     /locus_tag="ACG8EF_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947135.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="ACG8EF_001834"
     CDS             complement(958..1392)
                     /locus_tag="ACG8EF_001834"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4947136.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="ACG8EF_001835"
     CDS             complement(1624..2334)
                     /locus_tag="ACG8EF_001835"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS4947137.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="ACG8EF_001836"
     CDS             complement(2324..3184)
                     /locus_tag="ACG8EF_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFS4947138.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="ACG8EF_001837"
     CDS             complement(3189..3827)
                     /locus_tag="ACG8EF_001837"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFS4947139.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="ACG8EF_001838"
     CDS             complement(3842..4141)
                     /locus_tag="ACG8EF_001838"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4947140.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="ACG8EF_001839"
     CDS             complement(4184..5668)
                     /locus_tag="ACG8EF_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS4947141.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="ACG8EF_001840"
     CDS             complement(5688..6149)
                     /locus_tag="ACG8EF_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4947142.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="ACG8EF_001841"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="ACG8EF_001841"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFS4947143.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="ACG8EF_001842"
     CDS             7452..8222
                     /locus_tag="ACG8EF_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4947144.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="ACG8EF_001843"
     CDS             complement(8495..9322)
                     /locus_tag="ACG8EF_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4947145.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9890..11125
                     /locus_tag="ACG8EF_001844"
     CDS             9890..11125
                     /locus_tag="ACG8EF_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4947146.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12124..12384)
                     /locus_tag="ACG8EF_001845"
     CDS             complement(12124..12384)
                     /locus_tag="ACG8EF_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS4947147.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12411..13712)
                     /locus_tag="ACG8EF_001846"
     CDS             complement(12411..13712)
                     /locus_tag="ACG8EF_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4947148.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4510 a         2843 c         2185 g         4416 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta
     9361 tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt
     9421 aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt
     9481 gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa
     9541 agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa tgacgacagc
     9601 acacctatca attaatataa ttattttatt tttttatgtt ctcatgttta tttttaatga
     9661 cacaacttct cgtatttatc tggcacaaca tataaatcga tcaaaaaatt gttgcgtgtt
     9721 tttttgaaaa ttggagtttc aacttgatta caaaatttaa atttagaagt tgtgaaaaca
     9781 gcgagagaca gtttaatatt caaaatatac ataggctact cgaacgctgt gttttttagt
     9841 atactagtaa gaaacgaagg gatagactca ttccaataag gaggatttca tgaagacaat
     9901 tctttttgct cctgcagtat tcaatttagc tgagacaaca cgaatgattg aggttgccaa
     9961 agctatgaaa gaccgttatt actgtgtatt ctttggcttt tctaaaactt ttgctccttt
    10021 gatcacagaa agtggctttg agtatcattt actagcgccg atccttacga aaaaacaaga
    10081 agaacaaatc ataaaatttg accagttgcg tacacttaaa aaccctttta ccaaagaaat
    10141 ggttgcctct cgtatcacta gtgaacagag tctgattgct aagtttgagc ctcgactgat
    10201 tgtgacaggt tcgaatgtga caattttttt atcggcccgc aataaaaaga ttcctttagt
    10261 atatgttaaa ccctatgctt tgagtgaacc tttttttcaa tcagagcatg cgctgatgcc
    10321 ccacgagtta cgtttcttcg gccctttaag caatcccctt tggcactgga caaaaaagcg
    10381 tatagtaaat acccgatgga tccccaatgc cttcaaacac gctgcacaaa aagaaatgct
    10441 tcctttacca aaaacaagtt tacaaatgat ggatgctgat ctaaatctca tcacaacgcc
    10501 taaactattt ttggaagaag aacagctgcc ggaaaattat cgaatcgttg gtccaatttt
    10561 tgctaagctc agtacttcct tgcctgaaga agtgctaact tttatacagc gaaagcaaca
    10621 agaaagaaaa cgtattatct atttcgcaat gggtagttca ggcaatccgc gtatgttacg
    10681 caaaattttg ttcttcttac aaaaacaacc ggatctagcg gttattgcac ctattgacta
    10741 tctgcttgat gacattcaat ctgactcaga agatctttta ataacagaat atctgtctag
    10801 tcatcttttg catgaattca ttgatttttc ttttatccac ggcggggaag ggaccgtcca
    10861 aactgcctgt gaatccggca aaccgtttat cggtgtgggg atgcaatttg agcaaagctg
    10921 taatattgac tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac cagtgactga
    10981 aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc agcaggcacg
    11041 cacgctgcaa gccgtttttc caataaatgg tccacaacaa gcaattcaag aaatcgaaac
    11101 cttcctgagc aaccacccaa tatagtttca agtagaaaaa gcttttttaa tagagactca
    11161 tcagtaattt cgctattcga gacccagaca gtgaaaatac aaaaaaagta aatagtggat
    11221 tgaaaagtta gagttactta aattacggtg tctaaaaaac acaaaatcat ccttaaaata
    11281 aaagatctga cgctatccaa atgagcatag gtagcgtctg ccatttattc ttagtcaaat
    11341 tctttgacac ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa agaccataat
    11401 aattaggcaa atacattttc ttaaaatgtg tcatatagat ctagaagcat cgctttgtag
    11461 agatttgtgc cgctagtccc ctcaacaatt actaaatcaa atgtaggatt taccacttta
    11521 tctttgataa tcaacctccc tcttctgttg agacaatgat ctcgcaatcc gatgcatact
    11581 cctatataaa ctgccagcaa tccccacata ttgagtagat catgtcagaa tgttctatgt
    11641 cgtcagcaac gatacactaa aagtcacgtt caccatgact gattgctatt cccagcacaa
    11701 ttatttctat acacgtttca tgatttgaat aaacactcac atcatacata ttaccattca
    11761 tacaccatac tgcagcccct actatatggt tgtaagctcc caaatctata tttgacttga
    11821 tcactttggt agtagatcta ctaattctaa atccattgct gataaaattt ccattttttc
    11881 ctccttcttt ctgccaaaac agttaaaaca ataataatct ttatgtaaaa aagggctgtg
    11941 acaaaagcct tgtcacagtc ccatattccg aataaacggt ggtcaaaagc ttgcatctgc
    12001 ggtaataagt tcaaccaaac gaaaaataag ccaacatttc acaaaaattt aagaagcaat
    12061 tttcgtaaat ttcttcttat ttcttgaagc taaccacttc tgttacaacc acttttagaa
    12121 catttaaagc ttcgatgtga caaaccatct atgctgctcg atcataaaat tgatagcaaa
    12181 aattccacaa agtacatatg tcccccatga aaacaaaaca ttcatatgcg gtacaatgat
    12241 ctttggaaga atcacgatca aaaatgatgc cagtgcccaa atccatcgat tgatttgaat
    12301 atgatatgta gctagcatat tcaatataag gtgactagcc gttaataaaa agataaaaac
    12361 aaaaaataaa atcagccaat acattgtttc ccaccttcta aaaaccaaac ttagacagtc
    12421 tggacttcgt tttctccacg actttgttca tctgacagca tctttttatc ataagctttt
    12481 acaaatggat aatagataat aaatgctatc aatgtgttga tcatgatcaa gataacaaca
    12541 cgccagtcat ttcctgctga aagataaccg ccgattggag ctggtaatgt ccatgttggc
    12601 aaaacagtaa atctattgac catttctaat ttgactgcaa aataactgat aatagtcaaa
    12661 actaaaggaa ctaagttgaa cgggatgaac atgtaaggat tcaacatcat aggagctcca
    12721 aaaattatgg gctcattgat attgaagata ccaggaataa tagaaaaacg accaacctct
    12781 ttcaagaatt ttgatttaca gaagaatata aacaaaacag ataccacgat cgttgctcct
    12841 gcaccaccaa ttgttaccgt ccattgataa aactgttcag gagcgatata gggtaattgg
    12901 gtggcagctg taccatctgc caatgccttt gcattctcat ccaacatgat cagccacatt
    12961 ggacgaacaa cagcccctac aatactcatg ccatggatac caaatgacca caacaaatgg
    13021 atcaaaaaca ttggtagtaa aacaccaagc aaattattcc ctaaaatatt ggttaaaggc
    13081 tcgaataacg ataataaggc agcgtttaca tcaaagcccg cgacgcttcg aatcagccaa
    13141 gtaattgtta aaatgactgc acctggaatc aatgcctcaa aactacgggc aacagcaggt
    13201 ggcacttgtt ctggcatttt gatagtaata tttcgatttt taaagaaacg atatacttcc
    13261 actgcaaaaa tcatagataa gatccccgca aacatcccag atgctcccaa agaactcata
    13321 ggtaagaccc agccaagagg tgcctcttca ggagttaaaa cgtttacagg aacttgtaac
    13381 ataaagaacg ttcctaaaga taatatacca cctgtcacac tatctaatcc atatgattta
    13441 gctagatccg aacccataac aaaagttgca taaatagcca tcaatcccat cgtcatccta
    13501 aagggtaaaa caatcgttgc cgaataaggg gcaataaatt cattccatcc aggaaacggt
    13561 aaattgataa agaccataaa aaatgtccca atcaaaatca atgacaatgt tgaaacaacc
    13621 ccattacgaa ttgctaataa atgacgctgt gtaccaattt ttgccattgg tgggacaagt
    13681 ttctcttcga cccatttcat cagcttttcc actaaaacca ctccttttca ataaaatatg
    13741 aaccattcat tgcatgacaa cgcaaagaat gttcataact aattatataa ttaagaaatt
    13801 ttatttcaat atattctctt aaataatcaa atttattttt aagaatcctg aaaataagtt
    13861 ttctaaatca taggagcaac aaaaaaattc gataattaac aaaaatcaaa atgattaaat
    13921 ctagtgattg aaacatatca tttcaaaaaa attt
//
LOCUS       DAZUKZ010000065        13810 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000065 DAZUKZ010000000
VERSION     DAZUKZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.066"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(221..1423)
                     /locus_tag="ACG8EF_001847"
     CDS             complement(221..1423)
                     /locus_tag="ACG8EF_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4947149.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="ACG8EF_001848"
     CDS             complement(1426..1620)
                     /locus_tag="ACG8EF_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS4947150.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="ACG8EF_001849"
     CDS             complement(1667..2515)
                     /locus_tag="ACG8EF_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFS4947151.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="ACG8EF_001850"
     CDS             complement(2720..3070)
                     /locus_tag="ACG8EF_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947152.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3248..4111
                     /locus_tag="ACG8EF_001851"
     CDS             3248..4111
                     /locus_tag="ACG8EF_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4947153.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="ACG8EF_001852"
     CDS             4735..5727
                     /locus_tag="ACG8EF_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4947154.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="ACG8EF_001853"
     CDS             5743..6627
                     /locus_tag="ACG8EF_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4947155.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="ACG8EF_001854"
     CDS             6676..8283
                     /locus_tag="ACG8EF_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4947156.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="ACG8EF_001855"
     CDS             8284..9768
                     /locus_tag="ACG8EF_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFS4947157.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="ACG8EF_001856"
     CDS             9945..10937
                     /locus_tag="ACG8EF_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4947158.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="ACG8EF_001857"
     CDS             10989..11858
                     /locus_tag="ACG8EF_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4947159.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACG8EF_001858"
     CDS             complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACG8EF_001858"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS4947160.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DAZUKZ010000066        13717 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000066 DAZUKZ010000000
VERSION     DAZUKZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.067"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..475
                     /locus_tag="ACG8EF_001859"
     CDS             137..475
                     /locus_tag="ACG8EF_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947161.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(680..1255)
                     /locus_tag="ACG8EF_001860"
     CDS             complement(680..1255)
                     /locus_tag="ACG8EF_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HFS4947162.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1987..2568)
                     /locus_tag="ACG8EF_001861"
     CDS             complement(1987..2568)
                     /locus_tag="ACG8EF_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4947163.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2751..3377)
                     /locus_tag="ACG8EF_001862"
     CDS             complement(2751..3377)
                     /locus_tag="ACG8EF_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS4947164.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3399..4730)
                     /locus_tag="ACG8EF_001863"
     CDS             complement(3399..4730)
                     /locus_tag="ACG8EF_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFS4947165.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4885..5286)
                     /locus_tag="ACG8EF_001864"
     CDS             complement(4885..5286)
                     /locus_tag="ACG8EF_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS4947166.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5372..5578)
                     /locus_tag="ACG8EF_001865"
     CDS             complement(5372..5578)
                     /locus_tag="ACG8EF_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947167.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5673..7175)
                     /locus_tag="ACG8EF_001866"
     CDS             complement(5673..7175)
                     /locus_tag="ACG8EF_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFS4947168.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7193..7648)
                     /gene="argR"
                     /locus_tag="ACG8EF_001867"
     CDS             complement(7193..7648)
                     /gene="argR"
                     /locus_tag="ACG8EF_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4947169.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7746..8363)
                     /locus_tag="ACG8EF_001868"
     CDS             complement(7746..8363)
                     /locus_tag="ACG8EF_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4947170.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8584..9957
                     /locus_tag="ACG8EF_001869"
     CDS             8584..9957
                     /locus_tag="ACG8EF_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4947171.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10032..11144
                     /locus_tag="ACG8EF_001870"
     CDS             10032..11144
                     /locus_tag="ACG8EF_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4947172.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11216..12853)
                     /locus_tag="ACG8EF_001871"
     CDS             complement(11216..12853)
                     /locus_tag="ACG8EF_001871"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFS4947173.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13021..13629)
                     /locus_tag="ACG8EF_001872"
     CDS             complement(13021..13629)
                     /locus_tag="ACG8EF_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4947174.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3704 a         2886 c         2457 g         4670 t
ORIGIN      
        1 taaaaaaata atccaagaaa cattgaacct atggatccaa aagctaccca aatactatag
       61 ctaacccccc gctaccttca attttgcaga taaaacgctt tgggagaaaa tttcaaagac
      121 aacaggccat cctatcatgc ccgattataa acaaaaagct ggctattcct tttgctctga
      181 caaatttcaa ttgggaaagt tcacgaaact agcaggtgaa gaagtttcaa ctgttcggat
      241 caaagaatgc ccgcttcaaa tagaaacatt ggttcaacga atttcaacta gacaagactt
      301 tgctattgta gaacgtgaga tcgtagctat ttttgtagaa gaagggattt tacatgatcc
      361 aactcatatc aatgtggata aatggcaacc tcttatctat aaattcagag aatatacaac
      421 tgccaaccag tcgttaactt tgaattttag attccaagaa ttcaaagagt cataatgata
      481 aacatcgtta ggtataaata aaaaagactc gcagcacgag taaaaaacaa atagaagcta
      541 tacattagaa aggtcgactg tgaccctata gatgcagtat atgcagctta tgagcatttc
      601 ttctgttctc tttgtttttg ggatctgtgc tgaagtcttt ttcgtttttt gccgctcttt
      661 ctttgaaaaa tcgattatgt tataccttct ccattactaa ttcaggcatc tgctgagtaa
      721 tgatttttcg cgcaaaagaa atatctgtta cccaaggttc gatttgttct tcagtcaaga
      781 caagcggcat tcggtcatgg attggtgcga ctgtgcggtt aggttcagta gtcattatga
      841 ttgattctgc cattttttca tcctgatggc tacgataaaa accgccaata tacactattt
      901 tttccttatt tgaaaacata aatttgtctt tttcttcatt ccactcataa aagccactca
      961 ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg
     1021 tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat ccccacttca
     1081 tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca agatttgacg
     1141 gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc
     1201 gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca cacatcctaa
     1261 tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc aacaaaacgc
     1321 tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca gtgaatggta
     1381 ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgcttt
     1441 ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct gggtaaacgg
     1501 tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta gaaagccatt
     1561 ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct cttccttaaa
     1621 cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tatgagcagc
     1681 tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca acctctccac
     1741 taaacggtgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa aatatgagga
     1801 actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca caacctctaa
     1861 tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac aaaaatttga
     1921 gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc
     1981 taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt gaaaattctg
     2041 cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct ggatttcagc
     2101 aatcaacgct ttggcttgac ggtctgcttc ttcatcggac agtttttctt cagaaagtgc
     2161 caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta gcaacagacg
     2221 gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac agccatgata
     2281 agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta atgcttcttc
     2341 tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata cgtccatatg
     2401 cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat aaaattgacc
     2461 ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat agcctttttt
     2521 ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg atattctcct
     2581 ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct tcttaatcgt
     2641 ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg ttctcatata
     2701 gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc ttatgatagt
     2761 tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa gttcgtcaac
     2821 gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac cacaacattg
     2881 aatccactct ctaccaattc acgcatatgg gaatcgctac acaagtttcc gttcattcca
     2941 ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc attgctttct
     3001 ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata aggtttcaat
     3061 tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact ttttctagca
     3121 aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca tttatggtca
     3181 tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt tgcttgtttt
     3241 accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt tgtcaaacca
     3301 aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac tactgctgta
     3361 tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac atcatttaga
     3421 gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata aggaagtttt
     3481 ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc atagatactt
     3541 gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc tttaggatct
     3601 tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt gtaattgacg
     3661 ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg agtgataaat
     3721 acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc cttcaactga
     3781 tccataatga ttcgataatc atctagagag atataagtga actgcaatcc gcctttgacg
     3841 tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac agcgattgct
     3901 ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt acttggtttt
     3961 cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc atcttctgta
     4021 aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac acctagatgg
     4081 aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc ttcatcttca
     4141 cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta ttcattgaac
     4201 attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt ttccggtaat
     4261 tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt caatcctttg
     4321 attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc ttcaacaatc
     4381 actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg tgctgtaccg
     4441 tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc gcgaagcatg
     4501 cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct ttctccattg
     4561 aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc atacatacgt
     4621 gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt cgctaaagtc
     4681 ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat ttttccctcg
     4741 cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt ctattctcca
     4801 aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc acgtttttag
     4861 tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga tatttcctct
     4921 agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt gggcttcttc
     4981 ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc cgccatcatc
     5041 taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc ctgcttcttt
     5101 gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa ataattgttc
     5161 tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca tcgagccatt
     5221 tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt cgtatgcttt
     5281 tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt atagtgtatg
     5341 acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg ccgtcttcta
     5401 ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata tttgttccaa
     5461 aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga tacatatata
     5521 ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc gttaccattt
     5581 tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac tttttttgcc
     5641 gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga ttcaagatca
     5701 gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt tccgaattca
     5761 tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat caaaaaccag
     5821 ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc cgggtcgaag
     5881 ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt gtttccttgt
     5941 tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac gggctgtttc
     6001 aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg caaaagcatc
     6061 aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt gaatgcagcg
     6121 tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag ttctaatgct
     6181 ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc tttttcttca
     6241 atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt tcctggatgc
     6301 tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat cactgttcca
     6361 gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc aagatctgct
     6421 tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc aggatttggg
     6481 atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga atccgcacct
     6541 aagctattca atactttttc agcaaagaat ccaccagcac catttccggc atcaacaatg
     6601 attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt aatttttgtg
     6661 actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc agtttgttcg
     6721 ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc tgctcctcct
     6781 tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc agtcagcata
     6841 accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc aggcgtagta
     6901 gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat caacgcctct
     6961 ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca ttttttattt
     7021 ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc gatctttttt
     7081 accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat tcctcgaata
     7141 tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc atttattccc
     7201 tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg caaataataa
     7261 tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact tcatctagcc
     7321 aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg aactgaacag
     7381 actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca tttaaagaaa
     7441 cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga ctttttacta
     7501 acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg cctgcttcat
     7561 gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg atctggcgga
     7621 tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga ttatagcaaa
     7681 agataagtaa tatttcttca tattttggta ttttttactt ttattttttg ttttcattca
     7741 ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc aggtgctgat
     7801 tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc atagccttct
     7861 tttgcgatga atgcttctaa tttctcaaac gttgctggtt cttcggcata gggcccgacg
     7921 tgtaagattt gggcacaaag accttcttcc atctttttga atactagttg ttctttcaag
     7981 tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc tgtaacaaag
     8041 tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac tggttcatgc
     8101 aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata catctgataa
     8161 tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac tgcatataga
     8221 caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct gatgctaaag
     8281 aatggttttt taggaacctc tagtatttgc gggctttttt tcatactata gctcttgtct
     8341 ttacgccatt caattttttc catttttctt cctccgcgaa attccattta gttatttatt
     8401 atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc agtttgtgct
     8461 agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg agagagaggc
     8521 ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt agaggtgatg
     8581 taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac agggatgatg
     8641 tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac tcttatcaga
     8701 cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct tttgatgatt
     8761 tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa aaaactcttt
     8821 ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc tcctaccttc
     8881 atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc tcttccgttg
     8941 atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat gatgggtgtc
     9001 ggtactttga caacggccat tgctccagca attggcccca cttatggagg cattatgact
     9061 tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgat tctttctttg
     9121 attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta tttggattgg
     9181 ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt gagtacgttg
     9241 ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt ccttttttac
     9301 tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc aaataaaaca
     9361 ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg tgtatctttt
     9421 gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc tggattagcg
     9481 atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa aatgctggat
     9541 caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat tggttggcta
     9601 gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg tcatgtgttt
     9661 tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat gaatgcactg
     9721 aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca gttttctggc
     9781 gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca tacttcccat
     9841 aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt gttgctgttg
     9901 cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa agcgtagatt
     9961 ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg tttgtaaaag
    10021 gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt atgactctag
    10081 ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg acaggacagg
    10141 caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga aacaaacaat
    10201 ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa gatttccaac
    10261 ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa aaagatttag
    10321 ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt ggaaacatcg
    10381 aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca gcagtttcac
    10441 tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat cttggtcagc
    10501 gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat aagaagagcg
    10561 attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta attttgacac
    10621 ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat atacgtccgc
    10681 aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac tggcctgttg
    10741 ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc cttttaggtg
    10801 ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct tataagggat
    10861 tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac tggccatacg
    10921 agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttc ggcgtactgc
    10981 cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg accacaggtc
    11041 cttatatcgg ctatctatta gccaacttct taaatactgg tcattggtct acggaagatt
    11101 atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac agctttttca
    11161 agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg ttattttagt
    11221 tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctggcaag agatcttttc
    11281 ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc agatttttat
    11341 aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg atgtcttcaa
    11401 atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg attgacaatg
    11461 tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca tagcaggctg
    11521 tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga ggatagacac
    11581 ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt gtagtgatat
    11641 tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct aaaactgggt
    11701 cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa acagcatcga
    11761 tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg actgtccgcc
    11821 catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt gtacatgtca
    11881 cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac ggatagtctt
    11941 gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt cgagaaataa
    12001 cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt gctaaggtaa
    12061 tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc tggtcattac
    12121 tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt cccaatgccc
    12181 aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct agcacagccg
    12241 ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca tgtgtccatt
    12301 cgggatcaca aaagatataa agatctccct catcaaagga aacagacttt tgataagtct
    12361 ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa ttgttctcaa
    12421 atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg ccaacgatta
    12481 cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt aaaattttct
    12541 cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt gctgttcttc
    12601 ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa tctagttctt
    12661 cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt gctagttgat
    12721 aagctgtctt cctgtcaaaa acttcatgaa cattattacc gtaaaatgtc gtttctacaa
    12781 tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat tcgagactct
    12841 cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct ttattataca
    12901 ttattcgagt ttttatttgt actgctgcgc tgtgggaagc aaacgagtta tttttcacac
    12961 aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc atcattttcc ttttatcggc
    13021 ttagtaccag ccgtttgcta accagaatgt tttagcagca tcccatgaac cataacgaga
    13081 tgttacatat tgttctgcta cacgttcttg attggcagct gaatagtcgc cgtttaagta
    13141 tgaagcatct aattgataac ggccgatgta acgtccgtta gtagctgtgt atgaaccgcc
    13201 tgattctttt tgtgcgatcc attcttttgc agagcttgaa gttggcgctg ttgcagcagc
    13261 tggggcagca ggagctactt caactgtttc aacaggtgca acgtatgtat gttcttgcgt
    13321 tgctgtttca gttgtagatg ctgtattttc tgttgttttg ttttctgttg ttgctgaaga
    13381 atcatttgaa gttgggattg ttaattgttc accaacataa atgcggttga tgtttgcgat
    13441 cgaatttttt tcagcaattg catcgatcat tgagttgtct cctgcaaatt tttgtgagat
    13501 cttagataat gaatcacctg attttactgt ataaacttca tcagcgtgag catttgtagt
    13561 tcctacaaac attgcagtag caccagcagc aaaagttgta cctaaaacaa ttttcttaaa
    13621 tgaattcata ttaaactcct ttttttctcc gttttttatt ctttcgacgc tattaacttt
    13681 atcatttaga aatatttcat aggtaaaaga aacatga
//
LOCUS       DAZUKZ010000067        13400 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000067 DAZUKZ010000000
VERSION     DAZUKZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.068"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..714)
                     /locus_tag="ACG8EF_001873"
                     /pseudo
     CDS             complement(16..714)
                     /locus_tag="ACG8EF_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            793..969
                     /locus_tag="ACG8EF_001874"
                     /pseudo
     CDS             793..969
                     /locus_tag="ACG8EF_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1145..1714)
                     /locus_tag="ACG8EF_001875"
     CDS             complement(1145..1714)
                     /locus_tag="ACG8EF_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4947175.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1788..2111)
                     /locus_tag="ACG8EF_001876"
     CDS             complement(1788..2111)
                     /locus_tag="ACG8EF_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947176.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2265..3077)
                     /locus_tag="ACG8EF_001877"
     CDS             complement(2265..3077)
                     /locus_tag="ACG8EF_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947177.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3404..4279
                     /locus_tag="ACG8EF_001878"
     CDS             3404..4279
                     /locus_tag="ACG8EF_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFS4947178.1"
                     /translation="MSKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4293..4616
                     /locus_tag="ACG8EF_001879"
     CDS             4293..4616
                     /locus_tag="ACG8EF_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947179.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4613..5365
                     /locus_tag="ACG8EF_001880"
     CDS             4613..5365
                     /locus_tag="ACG8EF_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFS4947180.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5355..7526
                     /locus_tag="ACG8EF_001881"
                     /pseudo
     CDS             5355..7526
                     /locus_tag="ACG8EF_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7638..9809)
                     /gene="copB"
                     /locus_tag="ACG8EF_001882"
     CDS             complement(7638..9809)
                     /gene="copB"
                     /locus_tag="ACG8EF_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFS4947181.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9829..12015)
                     /locus_tag="ACG8EF_001883"
     CDS             complement(9829..12015)
                     /locus_tag="ACG8EF_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4947182.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12015..12224)
                     /gene="copZ"
                     /locus_tag="ACG8EF_001884"
     CDS             complement(12015..12224)
                     /gene="copZ"
                     /locus_tag="ACG8EF_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFS4947183.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12237..12677)
                     /locus_tag="ACG8EF_001885"
     CDS             complement(12237..12677)
                     /locus_tag="ACG8EF_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFS4947184.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12751..13290)
                     /locus_tag="ACG8EF_001886"
     CDS             complement(12751..13290)
                     /locus_tag="ACG8EF_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4947185.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3766 a         2807 c         2381 g         4446 t
ORIGIN      
        1 agaggggggg attttttatc gttgtggaat atttaaatac caacgcttgt catgaaaatc
       61 ttgtgctcca cccttagtat ttccttctgg atcgtttgtc gcacgcatca tgacgtatac
      121 tttcttatta gggaaatcac gcatattgaa agatacatga tagccaacat ttcctaaagt
      181 attataagct tgatttacgt ctggtctata aatcccatca gctcttactc tagctagttc
      241 ttttccagta ttgtagtcca taatgaaaat atactcgtat ttataattag caatgtgcca
      301 tccagctaca tgcaagtttg cgttttcgat ttctccgaac tgatcaatgt gggcgtaatt
      361 tgttccatct gtcagcgtag aatttgcagc acctgctcga gttggatcaa ttactggttt
      421 atcctccgaa gtagttggat tatcatccgt aaaaccatga gccaaatcat aggctagttt
      481 ttctttactt acacccattt cagaaagata accgtaagga tctgtatgat cgccccaaat
      541 attttgtgtt acccataaat gcgatttgat tcctggttgg ttataaggag tgtctaatgt
      601 taatggaata ccatatttca ttgctgaatc tcttgccaat tcaaagtata ctttatagtt
      661 cttttcaaaa gtttctttat catgtgtatg ttgtaactcg atttgcacag gacttttggc
      721 tcacgaggtt gtcctactct acatcttgct gatagatatg ctgaaaggaa aacaccaaca
      781 ttatgttttg ctttttcagc tagaacgaga caagcaggaa tacctttaga agatatcaaa
      841 ggttttttag gtcataaaga tgtttccact acacaagtct atgcccatat ttctcccgaa
      901 gtgaaaaaaa gatcaatgaa tcaactagaa aactatatag aagagcaaat aaaaaagcac
      961 tcaaattgag tgctttttta agctcactat cactaaaact atcactaatt ttatttataa
     1021 gcgaacatgt ttctatcact gattttttaa atctagcgaa aaacccttat cttaaaggcg
     1081 ttcgttcaat tctttggcaa ggtcttcaaa gcctggcttg cctagaagtg cgaacatggt
     1141 tttcttatag ctcatgtaga aaaattttct tgatgatgat tttatccatc aattgagatt
     1201 gactgttttg ctctcctatg atttcagcta tgattgtccg attttgcggt tttcttctta
     1261 actggtagtt gattttccca gtttccatca actcgctaag catatccacg actccgccaa
     1321 atccggtctt tttcgtaata aaacctagtg ctaccattcg ttcatctaga ttttttgctg
     1381 taatcgtcac accagtaatg agcgagtgct gtaatgtttg ggccagctct tccatccctt
     1441 cagcattgga tacgacggaa gctgttttgt taataggttc ttcgtttatt tcttttggtt
     1501 ctttcctctc cactttcact gtttgacgaa ggacatctag ttcttgcaag agcgtttcgt
     1561 attgttcttg ggtcagcatc acgccagcta ctttttcacg attaaatata tatacgccag
     1621 ttcccgcgtc tcgcgcttga gcaaatacat ccatcggcga tcgtttcact tctgtgatcg
     1681 aagatgtggg aacttctaat ttctttaatt ccaatggatt tcctccttta ctaatcgctt
     1741 tttattatag cacagactga cttgttttga tcttttttcc aaaggtttta tcggtttttg
     1801 cctaaccagt aattgatatc ggtaagaagt ctttcgatac caaatgaagg atggtacaga
     1861 tacgtttttt gatcttcgtt atggtctagt ggaaaaaaat gatcgatgat cactagatct
     1921 gcataccgag gcggcttatt tcgagtacat acatgtatag agatccgttt tcgatgtctt
     1981 agcgttttgt tcagtacccc agcaagtctt cttgcttctt ggtgttcata gcgaaaaaaa
     2041 aagacgattt tcacttcagg agggtggaca tctaaatacc ctcgttcctg gagtgttttc
     2101 agtaaaagca tcccccactc tactgcgggg tgtgcaagtg cttgttggat cgctagccga
     2161 aattgatata gtttatcttt ggaattggga aatttttcta atgtttcctg ataataacga
     2221 acttcttcta tcagccggtc atggatacca attacgtaac gaagtcattc ttggtaaagt
     2281 acttttgtca gctttgattt gctttcgctc tcaaattcgc tattcagcca aaacagcgaa
     2341 tcaagctttt cagtcacaat cgtcaatttc cgatagtctt gtgtctcttc tatccgtttc
     2401 caccgctttt cagtaatctg gttacagctc aagcgcaaaa ttgctagata atttttttct
     2461 tttattggca aaatctgctc ctctctaaat agcaattcag gaaacaattc aaagaatagt
     2521 gttcgactgt ctcctaaaat ctcgatttct gccggatcct ttctttgctt caataattgg
     2581 atgaacatct cagaaaagga ctgctctcca tcatctgcaa aaaaatgcgg aaaatctttc
     2641 aaaaagcaaa acagcagata tcttactttt ctttctgaac caaagatctg tgctttttta
     2701 taagaaaaag aaaggtggta ctcgccaaac cactttctca atcgctcttg cgtagaacga
     2761 tcgtacttct gctcgtaagg ttcattcaat agcagatttt tcagtaaaca aaacgggggt
     2821 gattggatca gaaaaacaaa caagagataa ggttcgctat atccttgatc ataaactaac
     2881 tgaacggttt tcttatgaag aatcagctga atgggacagt cgctttccgc caaacgtttc
     2941 agatgccgat ccactgtttt aacatcgatt ttcagttctt cagctaaatc agcttttcgc
     3001 cagatcaaat ctttcattag attgattagt gaaaataaat tctgtgcatt ttttgataat
     3061 agtttttgcc atctcatcga tcatttctca cctgatttct aaacatattg tttgatcaaa
     3121 atgggttcac tcctaaaaag ataaaaaact ccctttctat ttatttaaat acgcaaatct
     3181 tcctcattat tttttatttt actcaattta atcgtattct catttcaaaa cgaatgtact
     3241 ttaaaaaaac ataataagat cactttgctt gtatgatgta ttcccttcca ttacaatgaa
     3301 ataaatagcg attttatgag aaggtgatgt gaaaatggaa gaaaaaatag aagaatccaa
     3361 acaaaaaatc gatcagttga tataagaatt agaaaaagcg gtcatcagca aacgttcaac
     3421 tatccaattg atgatcgtgg ccctattagc aggtggtcat gtcttattcg aagatattcc
     3481 cggtgtaggt aaaacgttac tggtcaaagc actagcaaaa gctgtacagg gaaccttcag
     3541 cagagtccaa tgtactcctg atctgctgcc tagtgatatt cttggttttt ccgtatacaa
     3601 ttcacaatca aaagagtttg aatttcgtcc gggaccgatc tttaccacga tcttgttagc
     3661 agatgagatc aatcgtacga ctcctcgtac tcagtcagca cttttagaag caatggccga
     3721 gaatcatgca acgatcgaca acaatacata ccctctagac aatcattttt ttgtcttagc
     3781 tacgcaaaat ccaatcgagt acgaaggaac atatcctttg ccggaagcac agctcgatcg
     3841 ttttttattc cgactacaaa tcggttatcc ttcttttgat gacgaacttt tgctactttt
     3901 ggataaattc gaaaaagtgt tggataactt aaatgaagta ttgaacagtt atgaaataga
     3961 agaaataaaa acaaatgtgg cgaatgtctt tgtcagtccc gaagtggcat cttatgcctt
     4021 gcagttagtg tcagcaacaa gaagtcatgc tgccatccag cttggcatca gtccacgagg
     4081 aagcctttcc tttattcagg cagcaaaagc ttatgcttta atccatggga gaaattacgt
     4141 cacgcccaaa gatttgcagg atttagttcc ttacgtgttt tcccatcgcc tgatttttta
     4201 cgatcgttca ttgaaggaag acgaaaagaa acaaatcatt catactcttg ttgcacaaat
     4261 tcctattcct gtgaggtaga ttgaggtaga caatggaaaa aatcaaagat tatttcatta
     4321 attttgtgtt atttctggta tatttcatca ttgtgctttt tgctcttgta ttcaacaatt
     4381 ctttaggctg gttcttgttg gcctttttta ctctttatgt atcatggagc ttgatttcat
     4441 taagcagctt tctaaagaaa ctttcggtta cttgtacatt gcagccactc gtttatcgca
     4501 aaaaaagtat gcaggttatt ttttccatag caaaaaagag aactttttct ttacctctcc
     4561 ctcgtttaca aatcggcttc ccagcaatct ttgcagaaaa aaacaagaga ttttgattct
     4621 tacaaataaa ccgcaagaag tccaattgct ttggcagcca aaagaaagag gatgccatga
     4681 aacattaact gtcattttta cagcctatga tagtctagga cttttcagaa aaagtgcaaa
     4741 aagagagatc tcttttcctg tgactgttct gcctgctttt tgtaagattc aagcagaaaa
     4801 attacagaga gttctggaaa aaaaacagca gctgaacgcg taccaaagag gtcttgattt
     4861 tcgggaacat cgcgcttatc gtccaggtga ttctttcaac cgaatcgatt ggaaattgtc
     4921 tgctcatact caagagtggt tataccgtga atacgattat caagaagaag aatgttctcc
     4981 tcttttttgc ttttggggtt tgcccagtcc aaaatttgaa cctctgctag acttgtattt
     5041 ttctttatgg catttgagaa aagaaaagca gccggaactt ctgatattag gcaaccgcgc
     5101 ctttcatggc aaagaccccg gtcataccta ttttgcttct ttggaagctg gcgatgagaa
     5161 agcttttttc gcacacttga aaaaatatgc aaaaaaacaa atcgtcctct ttgttcctga
     5221 gaattctcct gaagtctcag aagctattga aacattgtca aaagatcgaa cggtttatct
     5281 tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa gacaagatat gttcattgcc
     5341 aggaggtgaa tggattgacg attaaacggg aaaaatggct gcgtggcgtt tactcctttg
     5401 tgatactaag tgtaggtgct ccgatatttg tagtagctaa tcaattgcaa aacagctacc
     5461 ttcttatcct aactattggt gtcctatgcc ttattgtctg ctgtattcct aaaaaatggg
     5521 tacgtttgct gctttctttg ccagtaatga taggttgttt gtttttatat tttccttcga
     5581 agacattttc catattatgg atcttacagt ttttttcgtc agtcacagag gaattcttcc
     5641 atctatccca aggtaaactt tattatttgc cagaaaagac agcttttttt ctcataatgt
     5701 tgctgatcta tttatttatt acattgaccg tggattacga ctattggtac gcaccctttc
     5761 tttctttgat ggcttacttc atgatgctta ccgtttttcg gaaaaaagat ttactgtacg
     5821 aaatgattgc ggtagtaact gttttgttcg tgtatctggc agcaaggcaa tttttctcca
     5881 tccggttgtt gtcgggaaac actcgattcc agtcattgac tacagttctg cttatttgtt
     5941 tcttaacttt tagcgctatc ttgcctttat atcttccgaa agtacaccaa tctctcgagg
     6001 aaacgagtga accgatccga gaatatctta atgatgaagg gctttatgac acactccatg
     6061 agtatcaatt aaccggaagt gcaagaacag gcttcagtga gaatgatgaa cagcttggtg
     6121 gacctttata tgataatggc gaagttgttt ttacagcgaa ccaaaaagga aatggctatt
     6181 ggaagatcga aaataaaaat cattacaccg gttctggatg gatagaagat caggacgcac
     6241 ccatccaacc aatccaagaa ttccctgttg acatcacgag ctaccaaacc gttatagaag
     6301 acaaagaaaa cattacattg accctaaaaa aacaagtttt cttaccttat ccttatggaa
     6361 atactacctt gactgatttt tcattagcta gtggacaaac gctttactat ttgcctgaaa
     6421 gccagcgatt cattcctgtc ccgaatgaat cgacacgttc tgcaacttta caaacagaaa
     6481 ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt aaaagctgat gatttagaaa
     6541 atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa acgtattgag gacttggcgg
     6601 tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt cgaagctgtc taacagtatt
     6661 taaaaacaag cggaaatttc cgctattcca aaacggatac cgggcatact ccaaaagaac
     6721 acgattatgt ggattatttc ctctttgatt caaaagtcgg ctattgtgac aacttttcaa
     6781 cttctatggt cgtccttctt cgaacacttg gtatcccagc tcgctggaca aaaggtttta
     6841 caaatgggct cagaacaggc acagataatc aagggaatga ggaatacagc attctcaata
     6901 gtcatgctca ttcttggcca gaggtatact ttcctggatt tggctggctt ccatttgaac
     6961 caaccccttc atttgccaat ccaatgggtt cacaaccagt tgctgaaaca accgaaaccg
     7021 tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat ggcagaaact atttccagtg
     7081 agagatctga ggtcattcca tcagaagaaa caacactcac accagcttat aaaatcgtct
     7141 tattcagcct tggagtgtgt ttatccgtct tgttgttctt tctattgtta ttcagaaaat
     7201 ctctgcgctg gacaagttat cttatctatc ttcatttttt tgcttctact aatacgattg
     7261 catatcaaaa acttcttaac cctttggaaa cattggtacc gcgcaaaccc gcagaatctt
     7321 tgcaggaata tagcctgcga atagaacaag aattcaagct agatctgcgt ttcattcgat
     7381 taactactca atatgaagca gaaatatatg gacgacaagc agatccggta aaattcgata
     7441 aaaaaaccta tttagaaata acgcatataa taagaaaaat ggtatacaaa aaaattctga
     7501 caaaagggag ccgcttcaaa aaataagata aaatttctgg agaatctact tctggaatgc
     7561 cgagaaccag ctgattgagt atcgattact cgaaaaaaag gttgtgacac gttttttgtc
     7621 acaacctttt ttcattatta tctgattttc aatgtcaaag catttagagc tacaactacc
     7681 gtacttaacg acatcaagac agcaccaact gctggactta atatgatacc aattggcgca
     7741 agaattccgg ctgctagcgg aattgcaaga atattgtaac ctgcccccca ccagaggttc
     7801 tggatcattt tctttcgcgt ttgcttggct agatcaagaa aatgcaaaat atcttttgga
     7861 tcgctgtttg tcaatacgac atccgcagaa tcgattgcga tatctgtccc tgcaccgatt
     7921 gccatcccga ttgaagcacg ggctaaactt ggcgcatcat tgatcccatc acccaccatg
     7981 attacttttt tcccttggtc aaggtatttc tggacaacag cttctttatc atctggtaat
     8041 aaaccgccat aatattcttc gatccctaaa taatctgcta ctgcttgggc agcgttttta
     8101 ttgtcaccag taagcatgac aggagtgatt tttctttctt tcaatgttcg gataaattct
     8161 tttgcttctg tttttactac gtcacctaaa gcaacgattg cagctagatg attttctaaa
     8221 atcaggaagc tgatggtatt tccttgttct tggtaaggtt tcaataatgc ttgttctacg
     8281 tttaatccga gacgctcaac ttctttttca ttaactattt tcacttgttg ttttttgact
     8341 gttgcctcta gcccgactcc tgaaagattt tttagatttt cagcttgata aggcttgatt
     8401 tcatgttctt tcaaataatt cataatgcca acagccaatg gatgattggc attcgcttcc
     8461 aaggctccta gatattgaag gatttcattt ttattgaacg catcatcaag tacttcgaca
     8521 cccgttacag taaattttcc ttctgttagt gttcctgttt tgtccagcat gatcacatct
     8581 agatcattgg cttgctccag tgcattgcgg tttttcaaaa gtaatccgtt tttagctgct
     8641 attgatgtgg aacgagcaac aactaatggg atagctaaac ctagagcatg aggacaggcg
     8701 atgataaaga ctgttaccat acgttctaat gcattaggaa gatctgtcaa gaacaaccag
     8761 gcgatgaatg ccaggatacc cacaattaat gcgacataaa acagccattt tgccactttg
     8821 tcagataatg actctagctt ggatttttct ccttgtgctt ttcgcaccat ttccataacc
     8881 ttcgctagat aaccattttc accagttcca gttacttcga tttcgatcgt tccatcccca
     8941 ttgatagaac cgccaatcac tttgtcattg gcttgttttt tgattcctct tgattcgcca
     9001 gtcacagctg attcgtcaac aattgtttca cctttcaaga ttacaccatc tgttggcatt
     9061 ttatcaccag accggacgat caaacgatca ccttcgttta cttctttcaa tgaaactgtc
     9121 tcttcttttc catctttcgt caagcgtttc actgattctg gcagtaattc tgctagtttt
     9181 tgtaaagcat tgctggcatt cgacacagca ttcatttcaa tccagtgacc tagtagcata
     9241 ataacgatca aagtagccaa ttcccagaag aaatccatga cgtgtgtatg tggattgagc
     9301 agatttgcga taaacgaata gacactataa atatatgcaa ctgtaatccc catcgcgatc
     9361 agtgtcatca tagcaggact tttttgtttc aattccatct tagctccact taaaaatggc
     9421 tgaccgccat agataaatag tatagttgct aagatcaata cgagccaatc agaaccagga
     9481 aacgttactt gaaatggaaa ttccattccc atcattggtg aaaaaagaat gattgggata
     9541 gctaggataa gcgatagcca aaatttttgt ttgaaattcc ccatgtgcat ggaatgatcc
     9601 atgccgccgt gttcgtgatc catgtgcatc gaatggtcca tttgtttgct gtttgctata
     9661 tctccatggt tatgtcccat gtgcatggaa tgatccatct gactatggtc atgtttcatc
     9721 tccatctgtt tttcatgaga ttctgtactt tctttgttta ctacttcttt tttatgctca
     9781 tgttttttct ccatatcgtt cccattcatt tttactccat cccttctctt actttttcat
     9841 gtgtcgattt aagcacaggg agttcaacaa tacactgacg gaactaaacg ccatcgctcc
     9901 tcctgcaatg attggattca aaaagcctag tgcggcaaaa ggaataccaa tcgtattata
     9961 gacaaaagcc cagaaaagat tttgtttgat ttttctcaat gtcaacctag acaactcgat
    10021 tgtttgtgca atggatgcca aactgctatt catcagcgtc acatcggctg tttccatcgc
    10081 aatgtccgtt ccacttccca ttgccatacc gacatcagct aatgctaagg ccggtgcatc
    10141 attgattcca tcgcctgcca taccgacttt tttgccatct ttctgaagtt tttctacgta
    10201 ggctgcttta tcctctggaa gtacttcagc aaagatatgc tttggatcaa taccaacttg
    10261 tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc agcataaaaa catctaatcc
    10321 tttattctgg agctgcttga ttgcttgttt gacttctagt ttgatttgat cagccacggc
    10381 aatcaacccg atcacttgct tttcatcagc taaaaacatg actgtttttc cctgctgttc
    10441 taattctaac gcgtgttctt ggtattcatc aaatgatagg ttcaattcaa ttaatcgttt
    10501 acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg ctgattccag cacctgggtg
    10561 ggctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc agttttccgt attccacgat
    10621 tgctttacct agtggatgtt cagaagcttg ttctaaagta taaaaaatag ataggacttc
    10681 tttagaaccg actagatctg taactttggg ctttccttct gtaatcgttc cagttttatc
    10741 caaaacaatg ctgtctaagt gagcagctgc ttctaatgct tctccgcctt taattaaaat
    10801 tccatttcgt gcaccaacac ccgttcctac cataatagct gttggagtcg ctaaacctaa
    10861 tgcacatgga cacgcaatga ctaatacaga gacactatgg atcaatgcga gttgccaatc
    10921 accagtgatc aaacctgtag ctatgagcgt gataaacgcg atgactaata cgatcggaac
    10981 aaaaatccct gagatccgat ctgcgatttt ttggattggg gctttgctcc cttgagcatc
    11041 ttccaccatt tgaatgattt gcgctaatac tgtctgcttg ccgacttgtg taacttccat
    11101 gtgaagtaga ccattcgtat tgattgtccc gccaaagagc gtgtcatccg gattcttttc
    11161 tacaggaaga ttttcaccag taagcatact ttcatctatc gcactgcttc ctgaaataat
    11221 tcgaccatct gctggaatct gctcaccagg acggatgact agttgatcac caacgactac
    11281 ttcttcaatc gctaacgttt cttctttccc gtttcttatc acttgggcgg tttttgtttg
    11341 aagagacatc aattgtttga ttgcattacc tgttttcgtt tttgctgtat gttccaaata
    11401 tttgccgagc aaaatcagcg tgatgatcat actgctgctt tcaaaataaa gatcttgggg
    11461 gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag gcagccgatg ttccaattgc
    11521 taccaataca tccatatttg gtgctttcgt cttgattgca tggtacgcac ctttataaaa
    11581 acgagcaccg acataaaatt ggacaggagc agacagcact aactggacga ttggaaaatg
    11641 aaaaaagtga acaatcgccg catggcttcc taacatcata gcaatcattg acaacatcaa
    11701 cggcaaagtc aaaaccgtac tcaatataag atcgaatagc atttttttca aatacgcttg
    11761 cttttcttct gcaatttttt gtttgtgggc ttcatcgtac aaaattgcac catagccgat
    11821 gttttctacg ctttggatta atttttcagt ggtcgtccct tcatatttga cacttgcttt
    11881 ttcagtggcc aagttgactg tggcattgac cacaccaggt tgtccgttca gctctttttc
    11941 gatcctagca gaacaatttg cacaagtcat acctgtaatg acgaacgtat ccattttggt
    12001 ttctgctgtt gccatcaaat cacctctgct ttataaccca atccattgat tgcttgacaa
    12061 atcttttcgg attgaacttt tgtttcatca aacttgaccg taccgtttgc ttttttcaaa
    12121 tttactttca ctttttgcac gccgtcaagt gcgcttactg tttcttctac tcttgctgca
    12181 caatgattgc agctcatacc ttcgattgaa aattgttgtt tcattttttc ttcctcctat
    12241 tgatgttgtt tacattcaca ttgacctggg atacagttac attcgatcgt atctactggt
    12301 tgcttttgtt ccagttgttt caaaatctca ttgatatctt cttgagtcaa agtggcttct
    12361 tctactagat ccgcaatcgt ctctccaatc ctttttgcac agatatggga aaaaagattt
    12421 tccgtcgcac tgcgaactgt ttccatctcc gagacagctg gataataaat gaattttttt
    12481 ccttcttgtt ctgtccagat cacttctttt tttaccaaac gacccagcaa agttttgatc
    12541 gttgctggtt tccaatccat cgaatcagct aagatctgcg tgatttcttg ggctgtcgtc
    12601 tgtccaagtg tccaaataac acgcatgatc tcccactctg aatcactgat ctttactggc
    12661 gttttgactt tttccattta tttcacacct ttcgtttaca aatgtaatca ttatcttatg
    12721 aatacagatt acacttgtaa acaaaaactg tcaattattt ttgctctcta ttttcaattt
    12781 agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg atcagtttat ttcttgcatt
    12841 agctacttgg atcagctgct gtattttcgg ttcattgttt tcttcaaaat gccagataaa
    12901 agataaaaat tctaagtatt ccctgtttag tttctccgaa aatgattcag gcatatctgg
    12961 ccgagatttt ttgtttatct ttctttttag agaacgccaa actactcggt agatggcgag
    13021 ataacgcggc actttaagcc aaatgatctt atctgcttct ttaatccttt cctctaatgt
    13081 agaagtataa tttccttcaa cgatccatga cggattagat accataaaca attgctgttc
    13141 ttgcaaaaac cacttttttg cctgaatact gtagtctgtt gtatgccata aaaggtctag
    13201 cggcagtagc ggaaagtcgg tgatttcttt gatcttcctt gcgagggtcg attttcccga
    13261 cccactttgt ccaatcacga tgatcttcat tcttctccct cacttctctt tggaatttgg
    13321 ttcgatcata ccacttataa cgccgcaaaa aaacaaggga ctgtgacaaa agtcgtgtca
    13381 aagtcccttg tttcgaataa
//
LOCUS       DAZUKZ010000068        13226 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000068 DAZUKZ010000000
VERSION     DAZUKZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.069"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            115..1854
                     /gene="yfcC"
                     /locus_tag="ACG8EF_001887"
     CDS             115..1854
                     /gene="yfcC"
                     /locus_tag="ACG8EF_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFS4947186.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2011..3195
                     /gene="iadA"
                     /locus_tag="ACG8EF_001888"
     CDS             2011..3195
                     /gene="iadA"
                     /locus_tag="ACG8EF_001888"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS4947187.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3306..3509
                     /locus_tag="ACG8EF_001889"
     CDS             3306..3509
                     /locus_tag="ACG8EF_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS4947188.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3610..4272)
                     /locus_tag="ACG8EF_001890"
     CDS             complement(3610..4272)
                     /locus_tag="ACG8EF_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4947189.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4285..5787)
                     /locus_tag="ACG8EF_001891"
     CDS             complement(4285..5787)
                     /locus_tag="ACG8EF_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4947190.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6335..8014
                     /locus_tag="ACG8EF_001892"
     CDS             6335..8014
                     /locus_tag="ACG8EF_001892"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS4947191.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8141..8620
                     /locus_tag="ACG8EF_001893"
     CDS             8141..8620
                     /locus_tag="ACG8EF_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFS4947192.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8839..9519
                     /locus_tag="ACG8EF_001894"
     CDS             8839..9519
                     /locus_tag="ACG8EF_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS4947193.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9527..10858
                     /locus_tag="ACG8EF_001895"
     CDS             9527..10858
                     /locus_tag="ACG8EF_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFS4947194.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10873..11835
                     /locus_tag="ACG8EF_001896"
     CDS             10873..11835
                     /locus_tag="ACG8EF_001896"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFS4947195.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11946..12284
                     /locus_tag="ACG8EF_001897"
     CDS             11946..12284
                     /locus_tag="ACG8EF_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFS4947196.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12363..13070
                     /locus_tag="ACG8EF_001898"
     CDS             12363..13070
                     /locus_tag="ACG8EF_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFS4947197.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4199 a         2335 c         2903 g         3789 t
ORIGIN      
        1 aagtgatttt ctaataaatt attcatgtgg aaatgtcatt tttactgtat aattcatttg
       61 attatccatt ttttattcat caaaattact gaaaaaagaa aggaagaaat aaatttgaag
      121 aaaacaaatt cttcaggaaa accgaaacgc gacttcacta aattatcgac accccatacg
      181 tatgtcatta tttttggtgt agtcatcttt gcatggatac tgacttttgt cgttccagca
      241 ggaaagttca gcacgcaaga tattgaatac aaagatgcaa atggcgaaac aagtactcgt
      301 actgttttac gtcaagattc cttccgttat gcttacgagc tggataagtc gtatgtgttt
      361 gatcaattag aagaactgca agatcatcca gcagaacgtg aaaaactgga tgttccggaa
      421 aaaggattgg aaaaagtcat tgctgacgga gaaaaaaatc ttacgcagga aaaactagat
      481 gaaatctcct tgacagatga tgtgctttac gatcagtacg gcgaaaatat ctatgatact
      541 tctaaaaaat tgcataaaac agccaaaata tggggaacag atgattttgg cggctttggt
      601 ttcttaaatt ttgtttttga aggactagta tctggagata agtatggatc tgctgtaggg
      661 atcgctgcat tgatccttgt tgttggaggt gcttttggta ttattatgcg taccggagcg
      721 atcgacgcag gtatctatgc gtttatcagt aaaactaagg gactggagcg tttagcttta
      781 ccgctattat tctttgcttt ttcattcggt ggcgctacat tcgggatggc agaagaggta
      841 attccattct caatggtgat ggttccattt gtcattgcgt taggatatga ctcgatcgta
      901 gcagtaaccg tgacttatgt cgcatctcaa gtcggaaatg cgacctcttg gatgagcccg
      961 tttagcgtag cggtggctca agggattgct ggaatccccg tgttgtctgg tgcgacattc
     1021 cgattgatta tgtgggtagt tgttactgca ttagccgctg gttatatgat gatatatgca
     1081 gaaaaaatcc gtaaaaaacc agaaagttcg ttgacttaca aatcagatga ttatttccgt
     1141 tctcatatca aaaagacatc tgatgaaaat aaaaaattca tgcttgggca taaattgatc
     1201 ttattagaga tgttagttgt ccttgtctgg atcgtttggg gcgtgactca aaaaggctat
     1261 tacattccag aaatcgcttc tcaattcttt gtgatgggat tagttgctgg aatcattgct
     1321 gttcttttca aattagatgg aatgaaagtc aatgacatcg cttcttcttt ccaatctggt
     1381 gcagctgatt tagctggtac agcaatcgta gtaggtatgg ccaaagggat tcttcttgtc
     1441 ttgggaggat ctgatgcatc tgttccttca gctttgaata cgattttgca tgggatcggg
     1501 acggcgttga ccggtgttcc agctgtcatt ggtgcttggg cgatgtacat cttccaaagt
     1561 ttgttcaact tggttgttac atcaaattca ggacaagcag ctttgactat gccaatcatg
     1621 gctccactgg cagatttagt aggtgtatcc agacaagtag cagttcttgc ttatcagctt
     1681 ggttcaggtt ttatggacgc ttttacaccc gtatcagcca gcttgattgg tgtgctaggt
     1741 gttgcacgga tcgaatgggc aaaatgggca agattccaaa tcaagatgca aggattcctg
     1801 tttgttttag gaactatttt catcatgatt gccattgcaa taggtctgca gtagtactgg
     1861 agacctgtaa ataaaaaagt aatccaaaca gtagataagg actccaagaa ccgtcatttt
     1921 acggtaaaat caaaggagta aagatcctat cgtttggatt attttttaag atcctatcat
     1981 cagttatact taaagaaaga ggaaaaatgt atgaaaatca ttcgacaaat tgaagtgttt
     2041 gcaccagatt acttaggaaa aatggatgta ctgattgccg gtgggaagat tttggctgta
     2101 gaagaacagc tagaaggcgg ctacgaagga gtggaagtag aagaactttc aggtgaggga
     2161 aaagtactaa ccccgggttt cattgactgt catttccata ttttaggtgg aggaggagaa
     2221 ggcggttatc aaaatcgaac gccagaagtc acattgagcc aattgactac agctggtgta
     2281 acgacagttg tcggttgttt gggaacagac ggagaaggta gagatatgac tgcgttgatc
     2341 agcaaagcga aaggtctgga agcagaagga atctccactt atgtatacga aggttcgtac
     2401 cgattgccgg taaaaacagt aacagattct atcatcaaag actttttgac aattgataaa
     2461 attatcggaa tcggagaaat cgcagtatcc gatcaccgtt cttctcagcc aagttttgaa
     2521 gagttcgctc gtgcagtagc agatgctcgt gtaggaggca tgttatccgg aaaagctggg
     2581 atcatcaatg tccacttagg cggaggaaaa agaaagctag agctattgac acgaatcgta
     2641 gaagaaacag aaattccgat cacacaattt ttgcctactc atgcaaatcg cacaccagaa
     2701 ttatttgaag cttgcgtggc atttgccaaa cgtggtggca cgattgactt cactgctagc
     2761 gaagatcctg atttttggga gaagacagat ggtgaagttc gcttcagcaa agcactgaaa
     2821 cgattgatcg aggaagatgt ttctttagat aatttcacga tgacctcaga tggacaagga
     2881 agcttgcctt attttgatga aaacaatcat ttcctagggt taggagtagg aagtgccaag
     2941 gcattgctag tagggatcaa agaagctgtg caaaaagaaa gtatcccttt ggaaatcgcc
     3001 ttacgagcta taaccagtaa tccagcacgt atattgaaat tggataaaaa aggaaaaatc
     3061 gaaattggtg ctgatgcaga tctgtgtatc ttggataaag aaacgcttga tattgataca
     3121 gttatcgcta aaggagaaat aatggtccaa gaaaaagaag tcaaagtatg ggggactttc
     3181 gaaaaaagtt tttaatcaac agctctaact atctttttta gaaaacgagc gttctgtttg
     3241 tctgtatctg ttgttgatgt taccctgaag gtagatcaat aaacaaacta agaggaggaa
     3301 gcacaatggc agatttaaaa ggacgttcta aagatatgaa agataaagta gtcggtgaag
     3361 gaaaagaagc atacggcaaa gcaacagacg acaagagcaa agaaacagaa gggaaagcac
     3421 aatcactagg tgctgacatc aaagaaaaag ctcgaaattt aggcgacgaa gtcaaagaca
     3481 agttttctcg aaaagacgaa aaaaaataaa aaccacttat tactaattga atcaagcaaa
     3541 taaaaaatcc gaaccatatc tgcgagacag atatggttcg gattttttat ttgatcatta
     3601 tcctgttttt taaacttcga ttttttgaaa ttttttattt tttagttcaa agacttcatc
     3661 acagcttttt gctacttcaa tctcatgagt cacgatgata atacagcgat tttgttcatg
     3721 ggcgatttct tggaaaagtt tgacgatatc ttgtgaagtt gtttcatcta gatttccagt
     3781 aggctcatcc gctacgatta gttgatgttc acagcagata gcacgaacga tagcgactcg
     3841 ctgctgctga cctcccgaaa gtttggtcac ctgttttttt gctaatattt catcaattcc
     3901 tacacgagcc agtcgatcta aagcaacttg tcgataattc tcttgcttgg tcttagtgat
     3961 ttccattgct gctatgacgt tgtctagagc cgataaataa ggaagcaagt tgtaagcttg
     4021 gaaaataatc gagacatcat ttttacgata acttcttaac ccgattttgt tcagtgaacg
     4081 atcttgatag tatacctttc cttcttttgg tgcatctagt ccagaaataa gcgaaagaaa
     4141 agtcgttttt ccagaaccac ttgctcctaa aatagcgtac atttttccag cttcgaaatt
     4201 cagttctaca tctttataaa gcgcatcttg cggatcttga taccaaaatc ccaattgttc
     4261 tgtccgtaac atcaaattcc tcctttagtt taataggatt tttcttgggt ttaatcttaa
     4321 tatccctata gaagctaaaa agacagaaaa cagactgatt ccaaatgcta ctccggctaa
     4381 tagtcccaat tgtgcaggtt tgacattgat ttccaattcg ctgacttgat cagaaacagc
     4441 aaatggatta ttcgtggttc cttgttgacc atcgcgttga ccgccttgtt cttgtctgtt
     4501 acctggcatt tgtccaccgc caggagcttg ctggttttgt gtcgtggttt ctgtctgttg
     4561 gtcaagtagc tgctgtccca cggcattcgc gacgacgttt ccgctaagcg aagcaactcc
     4621 taatgcaaga atcatacaaa tagccacttc ggtaaacaat tggagaatga ttttacttct
     4681 ggattctcct aaagatagca agacgcctaa ctcatgccga cgctcccgaa tggtaatcat
     4741 aatgatcaga gtaagtatga taataccggc tacagccact agtaaaacaa catttttaga
     4801 aaaacttgcc acgttgttta aaggttccaa cattgactga tacatggaat catttgattg
     4861 taagctaaat gtatctgtgt cgatcagctt ctcagcttct ttgagaaact catccatttt
     4921 atctggatca gatagagtgt aaatagcaga atctatcgtg ttatcgtcag atgtcccatt
     4981 tagttcattt gcaaaagtgt aagaagtgta aagtgtattc gacggattca tgaagttgaa
     5041 tttcataccc atactgccgc ttgtttcgct ggattcataa attcctttga tcgtcatttc
     5101 ataagtggta tcttcatctt cagtactcgt gattgtgaaa gtatctccga ctgccaggtc
     5161 atttgtttct gctagagtag aatcaatgac tacattgttt gtgcctttgt cgctgtcatc
     5221 tattccttca ccatccgtga tttttgctgt tccatcagaa aaattactat tttgtgcagt
     5281 tgctgacaca ccactgattt gaaagtctgc ttgagtcatt tgcggattcc cgcccattcc
     5341 tccagcaggt tcaccgcctg catcagtagt ttctgaactg tcttcacttg aatcagaact
     5401 agtgatggca gttattccat cttttgccat agctgaggcg gaactttcaa aggaataact
     5461 ctttacatta tctaattcag ctattttttc cgcgtccgtt aaggaaactg gtgtaagaga
     5521 aaaactgcct ggatctggac gttcttcagt ggtagaaccg cttgaagaag tttgttcctg
     5581 ttttttaaat gaagcttctc gattggcact taaagtcact gttgctccaa cttctttttg
     5641 tgcttgattc gctgcttctt ctgcagcgct tcgaatcgtg aggccagcta gcacaaaaat
     5701 aagaatggct gtgaacacgg caaacaataa caatgaacgt ccccatttta cttttgtact
     5761 tttccatgca cgtttcataa aattcatatt ttgcatccct cctagtagat gcatttgata
     5821 atagacaacc tatcttaaag ctagcttaaa tcttatgctg tttgcacacc atcaacatga
     5881 ggttctgtat atataaaaag tcactatagt tattgcaaaa aatgtggtaa aatgaaaaaa
     5941 gtatgtttca aaccgacgaa gaagaaatga aagaggaata gcaaaagaaa tcctagatgc
     6001 tacagaggga tttttacgtg catagttgtt ctaacaattg ctgatactag cgatttagtt
     6061 tatttgttgt ataataatgg ggaacagctg aatacttata taagtattct ggctatagcg
     6121 tttcgcaaaa gatccatttt gttaatgaag tatatccaaa ataaaatgcc catccaactg
     6181 tcagaacgcg gatgtgctgg taattttcct gctgattctc cataagttga gaaatagtga
     6241 ttttgcatat gcttcattgg aatggctcat atatttagat aaagaagaaa atcgacatct
     6301 ttaaacttgg atgtcataag aagaggtgaa gtttgtgagt accattaaaa ttatcccatt
     6361 aggcggcgtt cgtgaaaatg gaaaaaacat gtatgtcgca gaagtgggcg aggatatttt
     6421 tattttagat tgtgggctaa aatatcctga aaatgagttg ttggggatcg acgtcgtaat
     6481 ccctgatttt acgtatttag aagaaaacat tgatcgtgta gctggtgtct ttttaacaca
     6541 tggacatgca gatgcgatcg gggcacttcc ttacttgttg tcaaaaattt ctgtaccagt
     6601 attcgggaca gagttgacga tcgagttagc aaaattgaat gtcagtcgta atgaagcagc
     6661 caaaggattt aaggatttcc atgtggtaga tgagcataca gaaatagact ttggtgaaac
     6721 agtagtgagc tttttccgta ccactcatac gataccagat tcagtgggga tcaatctgaa
     6781 aacaaaagaa ggtagtatcg tttatacggg agattttaaa tttgaccaaa cggctatccc
     6841 aatgtatcaa acagattatg cacgattggc ggaaatcggc aaagaaggag tcttagcctt
     6901 actaagtgaa tcggcaaatg cagaaaatcc tcagcctgta gcttctgaag cccaaatagc
     6961 agatgaagta tttgatacga tcaaatattg ggaagggcgt atcatcgtcg cttgtgtagc
     7021 aagtaatttg caacgagtgc aacaagtatt ggatgcagca gaaaaagcac aacgaaaagt
     7081 tgtcttgact ggtcaagatt tcgaacgaat tattcgcaca gcgatgcgtt tagaaaaact
     7141 tcaattacca agtgaagatc tgcttgttac gttgaaagac atgaaaaact atgatccaga
     7201 agaactgatc attttagaaa ctggccgaat gggtgaaccc atcaagtctc tgcaaaaaat
     7261 ggcaaacggt acacaccgta atgtcaagat tgaagaagga gatttggttt atatcactac
     7321 tacgccaagc atcgcgatgg aaaccatcgt ggcgaaaacg gaagatatca tttatcgagc
     7381 tggtggaaca gtgaagttga tctctgagaa tatgcgcgtt tctggccatg ccaatcctaa
     7441 tgacttgcaa ttaatgatca atttgatgaa accaacatat tttgtgccag ttcaaggaga
     7501 gtatcgtgaa ttagctgctc atgcagattt ggctcatgca gtagggatgc catacaaaaa
     7561 tatctatatt actggacgcg gagacgtgct ggaatataag aacaagcgta tgagtgttgc
     7621 aggaactaca agtgcagaaa atgtaatgat tgatggatta ggcataggtg atatcggaaa
     7681 cattgtttta cgtgatcgaa aaatcttgtc agaagacggg attttcgtag cggttgtcac
     7741 tatcagtcgg cgagaaaaga aaatcatttc aaaacctcaa attacttcac ggggattcgt
     7801 ttacgtaaaa gctagccgtg atctgatgaa agaaagcagc aatatgattg aaacaatcgt
     7861 tgagaaacat ttagaaagca atgattttga gtggagcaaa ttaaagcaag acatccgaga
     7921 tcaattaggc cgttacttat ttgaacaaac aaaacgccgc ccagtgatct tgccagtcat
     7981 tatggaagct actcaacgtc gaaataaaaa ataaacaaaa agagtaagat gaacctctct
     8041 atagagtgct cgtcttgctc tttttttgcg aaaaacaaat aatttttgaa tacagcatcc
     8101 ctcgctttat aataaacgat agaataaagg gagtgaatca atgatgagtc aaacaaaagt
     8161 cgaacgttat ttagaagaac aaaagatcag ctatcaacca cttgatttta gtcatgtggt
     8221 agaagaatct ttcacagaag aactacaaaa aagagggatt gatccagctt tgatatgtaa
     8281 aactctcgta gtgaaaggaa acaaaactgg ggtgatcatc gcattgattc ctttgaacga
     8341 tcacttagat tacaaaaaaa cgagacaagt gacgaaagat cgtaaagttg gttttcctgg
     8401 aatggatttt gtattaatgc atacaggata tccccacgga gcaaacaccc ctattggaat
     8461 aaaactcgca catcctgatt attgtttttt agcagacagt tctttaaaag aaaaagaaga
     8521 agtaattgtg tcaagcggtg aaatcgggag aagccttcgg ataaaagtag ccgatttaga
     8581 aaagatcgtc catcctattt acgctgattt aattagttaa gggacgagaa gagaagctaa
     8641 gcagagcaga gacctcaaac ttgagattat atctgcttgg tacttggtgc atcctgtatt
     8701 atctgttaca attgctttgt acatactgta ctatcaatcc aatgaaaata tatttagcag
     8761 ctatattttt tcattgatgt gcaagtgatg aaatcgtttt taataaaaaa gatacagaga
     8821 aaacgcgagg tgtaaaatat gaaaattacg tatcatggac attcctgttt aacaattgaa
     8881 atggaaaatg gccaacgcgt gatgattgac ccgtttatta caggaaatcc attgacagac
     8941 ttaactgcag aagaagtaaa gacagattgg ttgctgatta ctcatggtca tagtgaccac
     9001 ataggagata tgattcctat tgcaaaagaa aatcaagcca caattatcag tattgttgag
     9061 attgctgatt atgcgaacag cagaggagta gattcattcg gtatgaatat cggtggaaaa
     9121 catgcgtttc cattcggcgt ggtaaaattc gtacatgcac agcatagttc aagctatgaa
     9181 gtagacggta tcgttcagta tatgggcgag cctagcggaa ttattattca agcagaagga
     9241 aaaacgattt atcatgcagg ggatactgct tatttcagtg atctaggatt gcttgcagaa
     9301 gaattcgata ttgatgtcgc tttcttgccg attggcgata actatacgat ggggccagaa
     9361 gatgcagcaa aggctgcgca aacaattcgt gccaagaaag tagttccaat ccattacaat
     9421 actttccctg tcattgaaca agatcccgaa tcttttgtag agttactgga agatagtgaa
     9481 ggaaaaataa tgagcgtctc ggaaacgctg gaagtgtagg cagaaaatgg cgacaaaaca
     9541 tgatttgatt ttagaatata ttgaaagttt acctgttggc aatcgaattt ctgtaagaag
     9601 catcgcaaaa gaattgaatg tcagtgaagg aacagcgtac cgtgcaatca aagatgcaga
     9661 aaatgtcgga ctagtttcaa caattcaacg agtagggacc atccgtatcg aacgcaaatt
     9721 aaaaaaacat attgaacgtt tgacatttgg cgaggtcgtc aggatcattg aaggggacgt
     9781 tttaggcggg gcggcaggat tagacaaggt cttgaataaa ttcgtcatcg gcgcgatgac
     9841 taaacatgcc atggaacgct atattacgcc aggatcacta atgatcgtcg gcaaccgggc
     9901 ggaagtccaa aaattagcat tggaagatgg cgccgcagta ttgatcacag gaggattcaa
     9961 tacgactgct gagattgcaa agctagctga tgatcttgaa atgccgatct tacgcacctc
    10021 ttatgataca tttacagtcg caaccatgat caaccgagcg ttaagcgacc aattgatcaa
    10081 aaaagatatc atgcttgtca gtgatatcta tatgccagct gaaaagactc attatttgca
    10141 gcagacagat acgatttctg attatcagcg tttgtcagaa gaaacgcagc attcgcgttt
    10201 tccagtcgtc aaccgccatc atcgtttgat ggggattgtt acggcaaaag atgttttagg
    10261 gaaatcgcca aaccagatca tcgatcgggt gatgacgaaa gaaccaatca gtgtaaagaa
    10321 aacaatgagt attgcctctg ttagccatca aatgatctgg gatgggctag aagtcatgcc
    10381 ggtcgtttcc gatgacttga cattggaagg cttgattacc cggcaagatg taatgaaagc
    10441 gatgcagctt gtccagcgac aaccgcagat tgcagatacg atttcggatc agatatctgg
    10501 aaatatccat acgattgata ctgaccgtga aggaaatcgt ttggaaaacc ctaagttcaa
    10561 attcgtagta gcaccacaaa tggtcaatgg cgttggaacc atttcttttg gcgtacttag
    10621 cgaaatcatt tctgatgtcg cacaaaaaac aatggtgatg aaccaaaaac gaaatatctt
    10681 gattgaacaa gtgaacctac attatttgcg gctgatccaa ttggaaagcg aattagacat
    10741 tcgtccacgt gtattggaaa tcggcagacg ttccgctaaa ctggatatcg aagtgtttat
    10801 tgaaaacacc atcgtttcca aagcaatcgt tgtttgtcaa gtaatggaac gttcttagaa
    10861 agcaggagtg aaatgacagt acagcaagaa atagtagata caataaaaaa atacgataaa
    10921 atcattatcc acaggcattt gcgccctgat cctgatgcat taggatcaca agtaggatta
    10981 gccgaactat tacgggaaag tttcccagaa aaagaaatac gtcaagtagg tgaaacgatt
    11041 gaagggcttc gctttttagc agaaatgcaa gaagttgaag atgtcttcta ccaaggagcg
    11101 ctagtcatcg tgactgatac agctaattca cccagaatca gtgatcaaag atatcagttg
    11161 gcagaaaaac tgatcaaaat cgaccatcat cccaatgatg acccttatgg agatcttgtg
    11221 tgggtaaata caaaagcaag tagctgtagt gagatgattg cagaatttgc tttgatgttt
    11281 cctgaatctt taaagatgaa cgttaaggct gcacggttac tttatgcagg aatcgtcgga
    11341 gatacagggc gctttttgta tccctccacg acgtcccgta cattagagat tgcttcgata
    11401 ttgagaaatt atccatttga tgcaagtgct ttgaacagag agattgaaca aatgccgatg
    11461 aaagtcgcta aactttccgg ttatctttat caaaatatcc aggtggatga aaatggtgca
    11521 ggaagaataa tccttccgca agaaattctt gataaatatg gaatcgatga ttcagagaca
    11581 gcttcgatcg tttcacttcc tggagtgatt gacgaagtgc tggcttgggg gatttttgtt
    11641 cagcagcctt cagggtatta ccgcgtacgt ttacgttcta aggggccgat catcaatgaa
    11701 ttggctaaac gccatcatgg aggaggccat ccattagcaa gtggtgcgaa tgcaaaagat
    11761 ttagcagaag tagaggaaat ctataaagag atccaagata tatgccaatc gtataagagc
    11821 agaaaaacag aatgatactt cgttcgaaaa cgtgcctttt gtagtaaaaa tcacaaatgt
    11881 atcttttcaa gatcttcttt ttccgttaca atagtgctca ctaaagattt taaaggagaa
    11941 agaagatgac attaccaaat tgcccagaat gtggctcaga gtacacatat gaagatcgag
    12001 gcttgttgat ttgcccagaa tgcgggaacg aatggaacaa agaagaacaa gaaactgtca
    12061 tagaagacgg attagtggta ttagatgcaa atggaaaccg tctgttagag ggcgatagcg
    12121 tgacagtgat taaagacttg aaagtaaaag gtgcgtcagg tgccattaaa caaggaacaa
    12181 aagtcaagaa catccgttta gtagaaggcg atcataatat cgattgcaag gtagaaggat
    12241 ttggaccgat gaaactgaaa tcggaatttg tgaaaaagaa ttaaagaata ttcaaggtct
    12301 tttcacattc ttttcctact actaatgata aaataaagta aacgaaaagg agtgaaacga
    12361 aaatgtatgg ttatggaatg tatggttttt tagatccgac atttcttttg gtcattcttg
    12421 gattagttat ttccggaatt gcgtcagcgt acgtcaatag tactttccga aaatacgatc
    12481 aagtgagaag caaaaacaat gtgactggta cacaagcagc acaatatata ttacaaagtc
    12541 aacagataaa taatgtcggt gttcaacaaa ttgccggaga tctcacagat aactacaatt
    12601 caggtaataa gatgttgagt ttgtctcaag caactgcaca gtctacttca gttgctgcga
    12661 taggtgtagc agctcatgaa tgcggacatg cggtacaaga tgcttcgggt tatgtcccat
    12721 tgaagattcg tgcagcactt gttccagcag ctaactttgg ttcgatgatt tcgttcccct
    12781 tgattatggt aggtgtattg cttagctgga actctacgtt aatcaatatc gggattttcg
    12841 ctttttcttt agcattactt ttccaattgg tgacattacc agtcgaattt aacgcgtctc
    12901 ggcgagcaat ccaaattttg agtgaaggcg gactactgac ggaagaagaa gtaccgatgg
    12961 caagacacgt attatttgca gcagcactga cttatgttgc agcagcgctt tcgacattcc
    13021 tgcagctgtt gcgcttgatc ttattgttcg gcggcaaccg aagagattaa aataaagaag
    13081 ttgtgaaatc agcgctttga tctgagctaa taccgagagt tagctgattg aataccattt
    13141 atttggaata agggctcttt gtcaataagg actgatgggt tgtacaaaat caaatctggg
    13201 tcagaatgaa cctcattctg gctcag
//
LOCUS       DAZUKZ010000069        13144 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000069 DAZUKZ010000000
VERSION     DAZUKZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.071"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            415..621
                     /locus_tag="ACG8EF_001899"
     CDS             415..621
                     /locus_tag="ACG8EF_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFS4947198.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(823..1824)
                     /locus_tag="ACG8EF_001900"
     CDS             complement(823..1824)
                     /locus_tag="ACG8EF_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4947199.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1829..3742)
                     /locus_tag="ACG8EF_001901"
     CDS             complement(1829..3742)
                     /locus_tag="ACG8EF_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4947200.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3910..4416)
                     /locus_tag="ACG8EF_001902"
     CDS             complement(3910..4416)
                     /locus_tag="ACG8EF_001902"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS4947201.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4576..5016
                     /locus_tag="ACG8EF_001903"
     CDS             4576..5016
                     /locus_tag="ACG8EF_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4947202.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            5175..6515
                     /locus_tag="ACG8EF_001904"
     CDS             5175..6515
                     /locus_tag="ACG8EF_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4947203.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(6584..7612)
                     /locus_tag="ACG8EF_001905"
     CDS             complement(6584..7612)
                     /locus_tag="ACG8EF_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947204.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(7667..7741)
                     /locus_tag="ACG8EF_001906"
                     /pseudo
     CDS             complement(7667..7741)
                     /locus_tag="ACG8EF_001906"
                     /inference="COORDINATES: protein motif:HMM:NF016901.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7744..8100)
                     /locus_tag="ACG8EF_001907"
     CDS             complement(7744..8100)
                     /locus_tag="ACG8EF_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947205.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(8398..9372)
                     /gene="sgrA"
                     /locus_tag="ACG8EF_001908"
     CDS             complement(8398..9372)
                     /gene="sgrA"
                     /locus_tag="ACG8EF_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFS4947206.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(9568..10407)
                     /locus_tag="ACG8EF_001909"
     CDS             complement(9568..10407)
                     /locus_tag="ACG8EF_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS4947207.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            10592..11479
                     /locus_tag="ACG8EF_001910"
     CDS             10592..11479
                     /locus_tag="ACG8EF_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS4947208.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(12063..12758)
                     /locus_tag="ACG8EF_001911"
     CDS             complement(12063..12758)
                     /locus_tag="ACG8EF_001911"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFS4947209.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(12742..13140)
                     /locus_tag="ACG8EF_001912"
     CDS             complement(12742..13140)
                     /locus_tag="ACG8EF_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS4947210.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3937 a         2647 c         2034 g         4526 t
ORIGIN      
        1 attatttttg tttcttattt tctaaaaaat taaaaaaaat tttggtgaat taagtatttc
       61 cttgataaca ctggcttttg gcctcatttt aggttatcat ttgtaatttt atttcacagt
      121 tgatatgatt gttatgtaaa ataaaaaagt ttaggataca tcgaattttt ggggaaattc
      181 ttttgggggt atcctaaatc ttttctatta tattcccata ttttctatta ttggtctaga
      241 cgtatgatgg taaactttgt acctatctga ttgctgaatc attaattgct tatcattagc
      301 catctttgtt tcaagttcgc tagatgatca ttttattcac atcctaacaa acttgtcaca
      361 tttatagatt cttaatctat ttatcataca attataaaaa aggaggtgct gcttatgaaa
      421 gctatccata tttctttgct ttttttcgct agtgcattga ttttatttgc tattcatctt
      481 tgttcccagc cttatatcaa taacaacgga atattagtgg aaccagcatt cttttgctta
      541 ccactcggct ttttgtccct tcttgcctcc gcaggcttcg gatttgttgc tttttttaaa
      601 cgatcaaaac aacaaatctg acttataaaa tttacgtaaa agagatcgtg acaataatcg
      661 tgtgatattc ccaaatttcg aataaaaggt gccccttgcc atctctaact cctcagtcac
      721 aagttacaat attcataaac catgaaaagc ttgtttattg aaattgctcc ttgtgacttg
      781 gagctaagtg acagattaca acctcttttg catcataaga ctttactcct tgaaaaacat
      841 tactaacttt tgatatgttt cttgcgcttg tttattgctg aaatcaaact gatattcatg
      901 gttcaaaaca gcatttttct ccgcataatc tacttttgtg actttcaccc ccgctttctc
      961 aaatacagaa gttacttctt ccatttgttt cgtaaacgta tttgtatttc catccgttaa
     1021 gaatgtgttt tgcgtccaac gatccgcatg tctgaccaag gctaaacttt tgaccttctc
     1081 tgattgttca aagtccttat tatcaaagta agcccatcca catttttcat aaagaaagct
     1141 agagatttta tcacttactt gagtaagtgc agaaggatcc aacagaccag acaaagaaat
     1201 aaacttcgta atctgcttgt tctccaaagt cggatcaaga tgatcagctt ttttaatttc
     1261 ttcattttga agtgctaaca aatattctcc actaatttgt gcacctgccg aatttccacc
     1321 aatcgaaata ttcgaccaat ctgcttgatc ttttatctct tttttagctg cttgaatggc
     1381 ttcgttcaat tggataactg gtgtaggatg ttttgcttcc ggtgatcgtt cataatctat
     1441 atttacgatg atttgctgcg tgtctgaaca tagatattcc aaataatcac gacaatcatt
     1501 tttatcccct cctacatatg cgcctccatg catccagaaa agaatcggta attttttctt
     1561 ttccactttc ggtgtgtaga tatccatatt attatttttg tactttgaag gataggatat
     1621 ttcttttttt atatcgattt gttcgatttt ttgctggaaa tcatctggtc gggcatatac
     1681 ttgttctttt tcacttgtgt caaattgttt tctaacaagc aagctaatcg gctttggtgt
     1741 gatttgatta atcaaaaata cagtgactaa aataactacg atgataactg gtactgcaat
     1801 cactattcgc ttgaaccatt tcattcattt accctctctt tcttcgccaa atccatgtga
     1861 taatcgtgct aacagataaa aatccacctg aataaatgag aagcgaatgt ttcttttctc
     1921 cagttttcgg caacattctt ttttttgcat ctgtcttttt ttgtactttc actgtcatct
     1981 gaatcggttg cgtatcatca cttgattgat cctctcgagg ttcattcttt cccttattgt
     2041 ctgcattctc tgtcggatct tgcttttttg gtggagctac tgggattttt ttgtaataga
     2101 aaataatagt tggtatcgtc tcattaaaaa tcccttcagt cggtccttca acatttgcta
     2161 attcgtatcc ttcgaatgtc ttttgctctg tcatataatt ttctccataa gctccgtaaa
     2221 gctggatttc gtcctggtct ttgagtagac ttttattgtt ttcatccaca tactttacgt
     2281 ttatctgatc atggaacact actttcaaaa agtccgttac atgtttcgtg taggcagcat
     2341 ggtcaacttc ctgtgccttt ccatgccctg ctccttctac taataaaaga tctttatacc
     2401 ctcctgataa ctcatataat ttttgcgcgt tagacacagg tactacatca tctgctgttc
     2461 catggataat caatttaggc aatgaatctg acattccaat cgtaggtaga gcagcttttg
     2521 tatccaaatg catctcctga tcaaaatagt attgtttcaa aaggcccata aataaatctt
     2581 catgctcttg atccccaatg atgtctaact gattttttat ggcatccggt agtgcggggg
     2641 ctactgtctg gctgtacgta aaacgtagtt gttcatcaat tgatgcaaat ccgcaatttt
     2701 caataattcc tttgacattg acaggaatat ctttcgctaa tacagacatc atcgtagctg
     2761 ctcccatcga accaccataa agtaaaatct tttgagatgg ccgctctcga acttcttgat
     2821 ttatccagta aaggacgtca tctgactcat attgtccata ggtcacataa tcgcctccgc
     2881 tattaccagt cgctctggaa tcaacaaata ataaattata cccggcctta taaaaatcat
     2941 tgtattcttc tgtaacgatc ccgttattcc aattccctcg aaatccgcca tggatcatga
     3001 ctgttttatc tgaaccttga tcgacataat atgcatatac atccatcatt ttgccatctt
     3061 gtttgtttag tacagtttga tgaagttgtt ttgtatttgg atagaagcct gtgttttctg
     3121 taggcgttgt cccatcatct gtacctccaa gcatcgacat caacacactg ttttttgcaa
     3181 aagcgatatc gtacaataga taagtagcaa tcagatgttt gataatctct ttatctacac
     3241 cataatcatt aacagcaagc tcagtgacta ataactctat taattttgac ttgatgtctt
     3301 ctgttggtcc attaagtatt tctgtcatct gtttaccgaa gtaattcaaa tattctttac
     3361 cattgaaatt ttcaccttta ccgatatcat cattttctgt cccattctct ttgtcactta
     3421 ctttagcaga agaggattcg tcgtttgaca tttttgtagc tgttgcttct tctgtttgtt
     3481 ggttatctgt ggtagcggtc gtttcttgat ctgtgctagt aacagatgtc tcaaccgttg
     3541 tttcagtatt tgtttctgtt tgtgaagtac tcgatttcgt ttcatttcca gcatcagtag
     3601 tttctgtggt ttctacctcc cgagattccg attcggaaga aatgctctct ccatatactg
     3661 tccattgcgg gacgcttgta cttacagcaa ataagatgac caacgctccc tttaatactt
     3721 gttttgttct caagctattc actccttttt atgtttctag gagaattata tgtaaacgca
     3781 aacatttttt atacaaaaaa taaatggttt atcccataaa aatagaacag gattattttc
     3841 tcattttacg taataaagca gaatggataa accagtatcc attctgcttt ttttaaacga
     3901 agaaacaact tattgctcca aaaatgtcag aaatcgttta tcccaaattt tctcgtaaaa
     3961 agcgattgtt tcctttgctt tcataaaagg attatcataa gttgtcgttg ctcctctaca
     4021 catcataagc tggtatccta atccatgagc aaacttcaca gtcgtatcta tacaatactc
     4081 tgcttgtgcc ccacataatt cgatcgaatg acagtccaat tcagttaata aaacactcaa
     4141 attagtctta taaaatgaat tcgcatgggt ttttctcaca aaataactgt gctcaggaat
     4201 agataattcc gacaatagct cccatggttt tgtttgtggc actaattgag catcttcgtg
     4261 ttgaacgaaa ataactggtt tcccaatcgt ttcgtatgtt ttgattcttt tattcactaa
     4321 tgctgtcact gtttctaatt cgtgtatcgg cgaagaatca aaacataccc ctttttgtaa
     4381 atcgatcact actattgcgt cggctgtgtt tgacatactt gtgccttctt tcttttttct
     4441 tgatttaata gtatttctta tctagctagt tccattatac gtgacaacat tttcttattt
     4501 tacacctcta aatggcactt ttcgactatt tcttcatgtt attatttcat aaatacttag
     4561 ctattggagg aatgtatgat gattaaattg tatacaggtt ctggctgctc gtcttcaaga
     4621 cgcgcaaaac gttggcttat cgtccatgat ataccgttta tcgaacagaa cttaattcgt
     4681 caaccactaa ccaaaaatga atttatccac ttactaaaat tgacaaacaa cggcgtgacc
     4741 gaaatgctct ctactcattc tattagttat aaaaatcttg ttcacagaat agatgaactc
     4801 agtttgagtg aactatatat tgagattcgt acatcccctt ctttattgaa aaaaccgatt
     4861 atctacgacc aatctgcaaa acttttgact ggatttagcg aagataaaat caggatgttc
     4921 ctttctaaaa ctaaaagaaa gcaagagctc aacgaactct tgctttctgg tcatcttgtc
     4981 gattttaaag aaaataatct ttttgcaatg tcttaatttt ggcataaaca atctgctttt
     5041 ctggatctat aatatctagt gttggtgacg ccttctgttg ccaatacttt ttcttttttc
     5101 taataaacgc aaacagacac cttgaaatcc tttagaataa agtcgacgaa aaccattcaa
     5161 aggagtgttt caagatgtcc tattcagagt ttaccaaaga gttactagat attctagacc
     5221 taaatcttac ctttcatgaa gatgcctttc gcaaagagcg cattaatgac gaaacatgtt
     5281 ttgtttttga tggcacattg acctatcaac cagaggaatg cttccattgc cactatcaaa
     5341 ataagcaaac gatcattaaa tggggctgga aaaaagtatc catcttattg aatgatgtca
     5401 gtaactacaa gaccattgtg cggattaaca aacagcgctt caaatgtaaa cactgtggaa
     5461 agactttttt agcagaagat tcagttagcg atcgtcgctg ctccattgcc cgcagagtca
     5521 aacaagccat tcttgagcta ttaagtgagc ctatttctat gtctttgatt gcacgtatga
     5581 agcacatttc tccgaccact gtcattcgaa tacttcgcag ccttcggcct aaaaccgttt
     5641 ctttgaacca gccgttacca gaagtggtgt gtttcgatga attcaaatcg gtgaaaaacg
     5701 tctcgggagc catgagcttt gtcatgatgg acggcaagac ccatcaactg attgatattg
     5761 tggaaaatcg gcaactcaat cctttacgcg actatttctt gcgctaccct agaaaagtcc
     5821 gtgaacaagt acgtctagtg gtttcagatt tctatacacc ctatcgcaca ttggtgaaag
     5881 agtgttttcc gaatgcccga atcgtcgccg atcgctttca tattagccaa cacatcggtc
     5941 gggcctttac caaccaccga attcaagtaa tgaagacctt taaaaaaggg gatcgtcggt
     6001 cgaagcactt aaagaaatac tggagactac ttcaaaagaa tgcgtgggaa ttgaatggtc
     6061 agcatcgcta ctggcgtccg tcatttcgag atcatctgac agaagcggag atagtcgatc
     6121 ggctattata ttatgatgac tccttaaagc gaggctacga agtctaccag gtttttcttt
     6181 ccgctatcag aagacaagat gttcctgaat tcgtcgcgct cctaaaagaa gactacaagg
     6241 agttgccaga gcactatcaa ccagtgttca cgaccttcaa gaaatatcga acagaaatca
     6301 aacgagcgtt gcgagttccc tattccaacg gccctataga atgtttgaac aaccatatca
     6361 aagtactaaa acgaattgcc tatggcttta gaaactttca aaactaccga gaacgaatct
     6421 ttttatatcg aggaaaatat ttcaagaaaa ctaagaacac tacccaactg actaaagcca
     6481 gaacaacaac acggctagac aagatagctg tctagccgtg tgagtatctc gattattcta
     6541 ttgaatttct tcaccaacac tcattgacga agagccgctt ttcttaatta acgaaatcca
     6601 atttttccaa tatggatcta tttttctcat tcctgatttt tatgatttca gctttcagac
     6661 gcgcccaatc attttttgta ggttgtccat tccagtaaaa tatctgtgta ttcgtttgat
     6721 catatgcctt tttgtcgata ggataatcgc taatgacaat atcaggtaac tgatcagcgg
     6781 atgcactata ttttattggg cgttcagtca tagataatat ccttttcttt gcccataatt
     6841 cctgtgtttt accatacttt gattgaatat agaatatgac aggttgttcg tgttttataa
     6901 aaatatccct gactaacata cagtattgaa aaatcaaccg tctgttcttt tcataataag
     6961 tattaagctc tggaattctg cgagctaccc cactaaaaaa ctgttgcatg attggataca
     7021 tcactggaaa tatatgctga aaatctctgt cttctgctga ttttccaaaa gcatccacct
     7081 tcttttttgt cccaaaaacc cttgaataca aatggatcat tgccaaattg acatatagat
     7141 aatttttttc tgaagttgta aaacaaatct tgaagttttt ttcaaaattt tctacaatag
     7201 acgattcaat ccagccccaa ctttcttgta ctttatctgg agtatccaac aaatcttctt
     7261 ccaaataggt gttcattaca ccaaacataa aataaaggta agcttgctct gttggagtga
     7321 tgtgattcat cgaccagaag tttgcttcaa aaaaaggctc tagtatgtaa tgcgtaaact
     7381 cttcttttgt tatcaatgga tgcacaaatt tgttcatctc ttctgggaca tacgtaatcg
     7441 agtggccttg tttcattcgt tgcaacgaaa ttcttagtag gctgatccat ttctttctat
     7501 ctatactacg cgtatatggc gcatgtttcg caaatttttc taatagttga cgtttatttt
     7561 ttatacttga taatatatca gaatgatttt cgtcgaatga ctgccaatac atcaagtgaa
     7621 aaaagtatcg gatacggtat tcttctcctt ctatcggatt accttttttc aagctgcaag
     7681 ataaatgata gtttttcaaa atagtattaa gttctcttaa ttttctttga atcgtacgca
     7741 aacttatata attttttgac taagattttc taaggattga taagtacctt tgtaaagtga
     7801 atccagtaaa atataaccaa tcgttccctc taaaagtata gaataaactt tactcagtaa
     7861 aaactgagaa gaaaactcga cttttatcat cttgttttct gaaagaaaag ataatttata
     7921 ttctggatac ctttcgcaaa acgattctgc ttgttgaatc accgaattca tcttagccag
     7981 ggtgtagttt agatctaaag cgatatcctt caccacatat aatttctcgt ctctttcata
     8041 acaatagttc aaaaattgat atacttgcct ttcattgata tcaataaatt cttcaaacaa
     8101 agtacatact cctttttatc tattatttag acttattccg acagctacat gaataacgta
     8161 caaatagatt cgttactgat ttttcagaga tactaccatg cgcagtgtgc attgtcaatt
     8221 attcttgctc ttattttcca tttgtgttcc tgttatttcc tgtttctcaa atatgttagt
     8281 aaacaaaaat agatctggca aagctacaac ttcgccaggc aatacagcac tctcgaatga
     8341 aatattcact caaaaatcta tcgttttatt caattttaga tctgttgctt ttattcttta
     8401 tttcactcga tggaagagag aacgaaaata gcttgctcct acaatcaaaa tgagtaacac
     8461 acccatccat gtaattagct tgttcaaggt tctactacca gtttctggta aatcgctttt
     8521 tctattatct gtttctttcg ctttttcttg atgatcaatt ccacgagaaa caaaatcagg
     8581 ctgttttagc acacttttta ggttgctccc tgcatttctc ttttgttcct tagttggtat
     8641 gataggtacc aaatcaagtt cattatttac ttttcctgtg ctgctttcgc tgctcgtgct
     8701 tcctgtgcta ctctcactac tcatgcttcc tgtgctactt tcactactcg tactctccgt
     8761 actgctttcg ctgctcgtgc ttcctgtgct actttcacta ctcgtactct ccgtactgct
     8821 ttcgctgctc gtgttttctg tgctactttc actgctcgta ctctctgtac tgctttcacc
     8881 gctcgtgttt tctgtactgc tttcactact tgtgctatcc gtactgcttg gcggtccaac
     8941 aactgtccaa ctgcttggtt tcaaattctc cgtgatcgtc atctgtccat cataaaaaac
     9001 aatcaaatca actttatctt tactcttgat ctcatcaatc gatagttctg cagaaatcat
     9061 tattgtatac tcaataccag cggtccattg tacgtctttt aaataaactt ccccaataac
     9121 tttttctgaa cttccattac catcccaacc atatgttaga acagcatgta catcactagc
     9181 attcacgctt ttgtttggtt tgactggaat agtaatgact tgttgtgtca tgtcataaac
     9241 aatccgatca gttttcacag taaatgaaag attctcattt gcccgttcat ttgcagaagt
     9301 actgattcca atcattgctg caattatact cagcccaaga ataacaaata atacggtcgc
     9361 tgttttcttc atttctatca ctctccatat aattaccttc tatttgataa aggaattata
     9421 taaaatgaaa atgatgagaa cacgacagtc agttgtgaaa atctaacaaa agatgtcgtg
     9481 tattcgcttg aatttgttta ctttttcgtt taatcaatga cagacttaaa aaagcaagac
     9541 cgatatcagc cttgctttct tcatctttta ttgataaaca gcagtgtatt tcttcatccg
     9601 ctttgtttta tatgcataga gtaacaaacc aacaatcaag actaacaaga ataatgtagt
     9661 tgatatagat gcttcagccc caaagtttcc cccagtaatt attgtatttt gcgattggat
     9721 cagagtggag aaaaaacctt gttcttgcgt tccgctgact ggtataccca aaatagctcc
     9781 ttgtgtccaa ttccaagtaa tatgagcacc aatagccgta aataaacttc ctgtactgta
     9841 gataagtgcg ctgaaaaagc aacctgctaa aaagatattg aaaactgcca gaaatgtcat
     9901 gttaggattc aatccgtgca aaagagcaaa taatgcagca gatccaatca ttgctattaa
     9961 cggaccaaat ctaagcatct gatgctgaaa aaagccacgg aataaaagct cttctgatgc
    10021 ccccactgca ataaataaaa taaaactcgt tatcagcggc acaatgtttg cgtttcctga
    10081 atctaaccga tacgcccccg tcaaatataa tggactagta ataagcagaa acactccaat
    10141 tccaacaacg agacctcccg ctaaataccc ccagtctttt cctcgaacag aaacaccata
    10201 atcttctatt ctttttcctt cgaccttact agcaagaaat aaagctagta aaaaaacagc
    10261 taaatgtccg accgtaccgg caattgctcc aatccattca ctatttccaa taaacatatc
    10321 cgcaagtgca ttattgacaa gaattcctaa aacaaaacca actaagacta ttaaaacaga
    10381 tccaatcaac cgaataatcc tgatcatctt tcatccctcc tttatattaa gtttaacaac
    10441 ccttttcttt cttgtctcgt gaaaactatg taaatgttct ccatcataga gagaatagat
    10501 ttttacgaac taagaatatc tacagcaacc ttacgaaaat gaaagactgt ctccctaatt
    10561 cttctgttag actgttttta ataaggaaag gatgggaaat tttttgaaaa aacaactact
    10621 aattatgtgc ctttcagcat gtgcaatttt actattctca ggttgtagca agtccaatga
    10681 aacgattgca acgtttaaag gtggaacact tacagttcag gatttttatg ataacccaaa
    10741 agttaaatca aataatcaaa cactattaaa aaaaatgatt gtcttcaaag catttgatga
    10801 tgtatacgga aaagagattt cttcaaagga agtaacaaaa gaatataatg aacaaatcaa
    10861 aaaactcggt ccaaactaca aggaacaatt aaaagcagtt ggccaaacag aagagacata
    10921 taagaaactc tttaaacaga tgctggcttt ccaatacggt ttgaaagcca atgtaaaatt
    10981 aacagataaa gatctagaca ctgcttggaa agaattctat ccagaagtta gtacacagat
    11041 cattctattt tcgactaaag aagaagcaga taaagcaaaa aaagaagcaa atgaagggga
    11101 aaatttctcc aagcttgttc aagcttatgg aaaaaataaa acattaaagg aaactgatgg
    11161 aaaaatgaat tttgattcaa ccaatccaga gattcctact gaagtaaaaa aagcagcatt
    11221 taaactaaaa aacggggaag tttctgatat cattcctgta acagatccaa cgacttatca
    11281 gcaaagctat tatctagtaa aaatggtaaa aaaacaggat aaaggttcta ataaagacaa
    11341 atacaaatct gagctagaaa aaattgctac cgaagcaaaa actcttgata cggaattcat
    11401 ggataaaaca atccgtaaag tgatgaaaaa agacaatgta actattaaag atccttatgt
    11461 aaaaaatata ttcaaataaa caaatttgac ccaattgggg atgtggcaaa atttgagaag
    11521 tgatttttgg aaattccttc ttatttttga ggcaaaccac ttttgccaca acttcatcgg
    11581 ggcctttttt tatttgtacc aaaacaaatc actggatttg ttacaatcat gaggatataa
    11641 tttaaaaaag ttttttattt ctatcacatg aatcaatgcg ccattaagta gattctttca
    11701 tgcttcccta ttttttcact tgccgtatcg cttcaaaaac aactatcata aactcaaact
    11761 cgactgaaaa gttattttaa aaaagactaa aaatatccct tctggacttg ttcctcttat
    11821 tctgttatac tagagaaaca cgaaagatat ttttatgcga cacttttcat ttgtttttat
    11881 gaaatttatt caaaactggc gtaacttcag agttacaagg atgtattaga ggttagggaa
    11941 tacatcgttt aaatgagaaa taagtaatag tcacccaaac tttaaattaa tgaatattga
    12001 atcgtcagat agcctcctcg agttttcgag aaggctatcg tttattttat ggagaaattg
    12061 attcagaagt aatcagcgac cttttttagt tcaccaatcg tttctgcttg gaaagtttgc
    12121 acccaatctt cttggaagcc cctcactgct ttatcaatcg agggcatctc taatacttga
    12181 tctagtagct ctttacctaa cgttatcgca tgagctccag atcgaatggc tgtagtgact
    12241 tgttcacaat ttttgaaact agctgctaat atttttgttg gtctttgcgt acgttcgatc
    12301 tcagtccgca acgaatcaat cactttcata gcatcaatgc cttgattttg catcctgtta
    12361 taataaggtg caatataatc tgctccatta ttaatagcta aatacccttg taattctgtg
    12421 taaatagctg taccggtaat caaataacct tcttttttca actttttgat cgcttgaagg
    12481 cctgcttgat tcaccggcac cttgataaac gtatcttttc ccagttcttc tatgataact
    12541 cgagcatcct ctatcatttc ttcttccgtt cttcctattg tctgaatatg gataggaatc
    12601 tgtccgatta tttttttgat ttctttcata tgcgagaaga agtttgacgc tccttctttt
    12661 ttcagaattg taggatttgt tgttacgcct gttaaattca aaaactgttg acaagaataa
    12721 atactttttg tatttgctgt atcaagcatg atttccatat agctttcccc cattctgatc
    12781 ctcttgatcg attttaatga ttgtcccctt tcttaaatgc ggcatttcct tatcttctac
    12841 atacattgtt cctggaagtt ccggctcatg agatccatca aattttatcg tgatatgtcc
    12901 taattcattc aaattagttt ggacaacatc tcctactgct gtgattacat acatttgttt
    12961 atcaaaacag agcttcatat cacaagttat ttcttttctt acctgattca attcaatctt
    13021 ataacaataa tctgttaatg tttccggtgc ttcatctcca aataaaatca ttattttttc
    13081 tttcctgaat acttctgctt ctataccaac atctgttaca accgtttcaa agatcgtcat
    13141 ctct
//
LOCUS       DAZUKZ010000070        12776 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000070 DAZUKZ010000000
VERSION     DAZUKZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.072"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(330..692)
                     /locus_tag="ACG8EF_001913"
     CDS             complement(330..692)
                     /locus_tag="ACG8EF_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947211.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(766..1146)
                     /locus_tag="ACG8EF_001914"
     CDS             complement(766..1146)
                     /locus_tag="ACG8EF_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFS4947212.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1228..2403
                     /locus_tag="ACG8EF_001915"
     CDS             1228..2403
                     /locus_tag="ACG8EF_001915"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFS4947213.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2480..3064)
                     /locus_tag="ACG8EF_001916"
     CDS             complement(2480..3064)
                     /locus_tag="ACG8EF_001916"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4947214.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3265..4263
                     /locus_tag="ACG8EF_001917"
     CDS             3265..4263
                     /locus_tag="ACG8EF_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4947215.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4375..4884
                     /locus_tag="ACG8EF_001918"
     CDS             4375..4884
                     /locus_tag="ACG8EF_001918"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFS4947216.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4914..5573)
                     /locus_tag="ACG8EF_001919"
     CDS             complement(4914..5573)
                     /locus_tag="ACG8EF_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFS4947217.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5575..6339)
                     /locus_tag="ACG8EF_001920"
     CDS             complement(5575..6339)
                     /locus_tag="ACG8EF_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFS4947218.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6347..7024)
                     /locus_tag="ACG8EF_001921"
     CDS             complement(6347..7024)
                     /locus_tag="ACG8EF_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFS4947219.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7061..8446)
                     /locus_tag="ACG8EF_001922"
     CDS             complement(7061..8446)
                     /locus_tag="ACG8EF_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFS4947220.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8656..8740)
                     /gene="ffs"
                     /locus_tag="ACG8EF_001923"
     ncRNA           complement(8656..8740)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8EF_001923"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8763..9278)
                     /gene="tadA"
                     /locus_tag="ACG8EF_001924"
     CDS             complement(8763..9278)
                     /gene="tadA"
                     /locus_tag="ACG8EF_001924"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFS4947221.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9385..10242
                     /locus_tag="ACG8EF_001925"
     CDS             9385..10242
                     /locus_tag="ACG8EF_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4947222.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10323..10658)
                     /locus_tag="ACG8EF_001926"
     CDS             complement(10323..10658)
                     /locus_tag="ACG8EF_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947223.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10725..12095)
                     /locus_tag="ACG8EF_001927"
     CDS             complement(10725..12095)
                     /locus_tag="ACG8EF_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFS4947224.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12173..12709)
                     /locus_tag="ACG8EF_001928"
     CDS             complement(12173..12709)
                     /locus_tag="ACG8EF_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4947225.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3805 a         2589 c         2125 g         4257 t
ORIGIN      
        1 actcagttat cttatcatgt cgttagtaac atgtcaataa ctatttttta aattttttag
       61 aaatgtgata taacatccac ttcagaatca acgctgttaa atttatcatg tatttcttcc
      121 ttggtcaact ataaattaag cattactttg tgaaatgatt tttcgatcaa ttattattat
      181 tcgttataca accgttttca acatattttg aaaataaaat gcactaaaaa actatcttta
      241 gcagaacatt tcattgtatt ttttggacca aacaataaaa aaagaagttt gaaaaaaatt
      301 caaacttctt tgttcgctta atctgtattt tagaagaaag gcactccctc tacttcgtaa
      361 gttccaagaa cagagcgcat ttgacctggt tcttcccaaa tatatccttc gcctaattct
      421 tctctttgat tcttttgttc agtttcaaag acaaacaacg gaacattttc ctctttaata
      481 tgtgtatttg tcaattccat caaactaatc ttgctaacat cgatatgaac tatttctttt
      541 aagaaattaa gtatattcgc taaaccagtc tttccttcag aaagttccgc taacgcaaat
      601 tccaacttgt catcaattga atggaccaaa aattttttag taccatcctc caaatgcaac
      661 ataattgtac cagcaactct tttgcttgcc aaaatttcca cctcgatcaa cttgttctat
      721 ggcttacata aaaattctag cacattttca gaaaaaatac actttttatt tcttattttc
      781 tttccgttct agttcagata atgcttcttc tatccaaata ggtaactgct ccgcaatagt
      841 ggtaaatccg atcttacgat ttttattcgt gaaatacctt tttctgcgat atggaacagc
      901 tggatttgtt ttttctaacg ttcttaaaga taaactgatt tttttggaat attcatcaat
      961 gtcgatgatc tgaactgtta ctggctgtcc gacttttaac aatgaatgga tatttttcgt
     1021 ataaccggat tgtacttctg aaacatgaat aagtccttgc gtctcgttat caagagaaac
     1081 gaatgcgcca taaggctgta tccctgtcac ttttccttca attatttgtc caattttata
     1141 agtcatctac ttccctctct tcgcatttat cattatactg taaatagctc aaaaaaagaa
     1201 gcgacgaaaa tgactggagg tcttaagatg atcgaatttg ataaacagat ttcaagaaaa
     1261 gatacagaaa gtgtaaaatg ggacgcaatt gctgagacat atcaaatgga tgacttactt
     1321 cctttgtggg tagcagatat ggacttcttg tctcccggtg gggtagctaa agcgtttagt
     1381 gaatacatca aacatggaat ctttggttat gccactctgt ctgataaact atatcaggct
     1441 atcattcatt gggaacgcca acaccatgct gtggagttga cgaaagaaaa tatcgttttt
     1501 acaagtggtg tgttaaccag tttagctgca gcagtacaaa cttttacaaa accaggcgat
     1561 tctattttga ttcatgaccc agtttatcct ccattttcct ctattataga aacaaatcaa
     1621 cgtcacatcg ttcgaagctc tttgctagaa aaaaataatc atttcatcat ggacttgtct
     1681 gacatggaga aaaaaatcga agaaaacaat gtaaaagcaa tgattttatg caatcctcac
     1741 aatcctggag ggcgtgtttg gagcaaagaa gaattgaaac agttaagcga attgtgttta
     1801 aaacatcaag tgattctgtt tagtgacgag atccatcaag atttaacttt attcgatcat
     1861 acatttactt ccatgctaac gattgatcct aatttagatg atctactagt tgcttttact
     1921 tccgcaacca agactttcaa tctagctgca atcaaaaatt caatggtatt tatcaaaaat
     1981 ccagagatga aagctgattt tgaaaaacat ttagtgatga accaacagca cgaaatcaac
     2041 acttttggac tgattggaac ccaagcggct tatgagacgg gtggagaatg gttaagccaa
     2101 ctgattcctt atctagaaaa aaatgttcaa acagttaatc agttttttga acagtatctt
     2161 ccgaaagtga gaataatgaa ccctgaagga acttacttaa tgtggttaga ttttaccgcc
     2221 tatatttcag acgaccaaga attggagaac actcttgttc ggaaaggaaa agtcgtatta
     2281 aatcctggca tcacgtttgg tccttccggt catgggcata tgcgcttgaa tattgcttgt
     2341 ccagaagaaa ctttactgga aggacttaat cgaatcaagt atgctttaga gacaatcgat
     2401 taaatcattt attcaaaata aaaggacgag accttactag gtttcgtcct ttttcataat
     2461 tactccgtac cataccaaac tattctgaaa tttcaattgt ttcaatcact acatcatgta
     2521 caggtcggtc ttgtggacca cgttgtacat ttgcgatttc atctactgtg tccatccctt
     2581 ctagtacatg tccaaagact gtatgtcgga aatctagcca aggagttccg ccacctttat
     2641 acgcttcaat gatttctgat gggaatccag caccttctag ttgtccaagc atattagctg
     2701 gcacatttgt gttatttaca ataaagaatt ggcttccgtt tgtgtttgga cctgaatttg
     2761 ccatagataa agctccgcga aggttgaaca gttcacgact gaattcatct tcaaaacttt
     2821 cgccataaat actttcgcca cccattcctg ttccagttgg atcgccgcct tggatcatga
     2881 aatcaggaat aacgcggtga aaaatcacgc catcataata accttttttg gaaagttcaa
     2941 tgaaattttg aaccgtcttc ggtgctagtt cagggaataa ttgaacagta atgtcaccac
     3001 ggtttgtttt gataacagct tttggtcctt tttcactttt taaatctagt tgtgggaatg
     3061 ccatattgtt tcctcctaat atttgcatac ttcataataa taataacata atacctaacc
     3121 tctcgtaaaa atactagatt tgttttcttt tatttttcat cactagatac attctataac
     3181 ttaccgtttc gatttcgaat tcaaacattt ttgagaagca ttctcaatta aattctgcta
     3241 tagtgtgaaa agggaggaat ttttatggaa atctatgaca ttacaatcat cggtgcaggt
     3301 cctgtaggga tgtttgccgc tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt
     3361 gacagtctac ctcaacttgg tggtcaatta gcaactttgt atcccgaaaa atatatttac
     3421 gatattcctg ggtatccagc aataaaagca agtgaattaa tcgatcaatt agaaaaacaa
     3481 ttaactactt ttaaccatac attccattta aaagaagaag ttctctcgct tactcgtgaa
     3541 gacgaagtta tcgagattac tacaaataaa ggcatccatt attccaaagc agtcatttta
     3601 gctttaggaa atggttcatt ccagccaaga aaattgaatt tagataatgc tgaatcattt
     3661 gaaaaccacg gattggatta tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc
     3721 gctattgctg gaggtggtga ttctgctatc gattggacgt tgatgttaga gcctattgca
     3781 agcgaagttt acttgattca tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca
     3841 cgcttgaaat cttcatctgt caacttgctt actccctatc tcatcgatgg attatcaggc
     3901 aataatgggg aactgacaga tatccggtta aaaaaagtga aaagtgacga gacgattgat
     3961 ttgatgattg attctctgat cgtcaattat ggattctctt ctaatttaga acatctttct
     4021 tcttggggac ttgatagtac cagaaatgcc attactgtaa aatcagatat gtctacttct
     4081 attccaggcg tttatgcagc tggtgacatc tgtacctatg aaggaaaagt gaaactgatt
     4141 gctactgggc tcggtgaagc acctactgca gtgaacaacg ctttacacta tattaatcca
     4201 aaggaacgaa ctcagcctgg tcacagcaca agcttatacg ataaaaatct tcgtcctagt
     4261 tgatttttta tatctctttg catttcaagt taaacccgaa agcagtttct tgcatttttt
     4321 tcatgttccg ttaacatgaa atagagaaag aaaaaataaa gggggagcaa taatatggtc
     4381 gtcaaaactg agactttaga agaaaaggca agagaactat tgaaggaacg tggcgtaact
     4441 gtagaagata tcgcacaact tgtcatgttt ttacaaaaag actatatttc tgatttgact
     4501 ttggcggatt gtactgaaag tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc
     4561 attatcactg gaatccaatt agatatatta gcagaaaaga aaaaattgat gagtccgttg
     4621 caacatatta tcgaagatga cgaaggcctt tacggtatcg atgagatcct agccttgtca
     4681 atcgtgaatg tttacggtac gatcggcttt acgaattatg gttatatcga caaggtgaaa
     4741 cctggtattt taaaagaact aaacagccac gatggcgttc atgtccatac cttcttagat
     4801 gatatcgtcg gagcgatcgc cgctgctgct gccagtcgat tagcacacaa agaaccagaa
     4861 aaaagaagtc gccttactca ataattcaac aaaaaaagaa agaaaacgtc tatttagacg
     4921 agttctttct tttttgtttg tttcaagcta ttttttccta agaaaagaaa aacagcaaaa
     4981 aataattcta ataatacaaa aaataagata aagctatgca tgaaaaattc ttctggaagt
     5041 acattgatga ttggatcagt aacgggatcg aataaccagt catcgttgtt gaaaaacgtt
     5101 tcatgaaaaa ggataaaaaa gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact
     5161 ggcagtaaca tgccgatttg aaacggacga atcaaccgcc aaagccgatc attcttttgc
     5221 aaatatttga taaacaaaat actcgggatc aaagtgatga aaaacacagc ataatccact
     5281 aaaaacaaaa ttttcacttc ataaaagtga tgggctccgc tagcagaaac tgggaaatct
     5341 ggcaagctga ggatggtttt gaagggatta ttgagataat tcattaggtg atcaaaattt
     5401 ttcagtaacg tctcttgatc taaagaagta taatccaaaa tttgcaaatg tccgatatca
     5461 aatacgtata gtggtcgaaa gttgatcgtc acaccaactg ccagactgat cagtgtcaga
     5521 aataggctga acaatccagc atattccatc cactgccatc tcgaattttt catattagaa
     5581 gctccattca tccagtgatt gtttcacata ggtagctggg ataggcaacc caggtacatc
     5641 ttcttctttt gtaaaacctg ataaaacgag caaagtatct atattattgc gtatcccagc
     5701 ttggatatct gtttcatagt tatctccaac catgattact tcttcttttt gcagccctaa
     5761 tacttcaacg gctttatcca taatgatggc ttctggtttt ccgatgtaga tcggcggcgt
     5821 ttgggttgct gtctctacaa aagagatgac tgaccctgct ccaggaagaa ggccgcgttc
     5881 agtaggtata ttcttatctg ggttcgttcc aatgaaagta gcaccttttt gaatagcaag
     5941 tgttgcttta actacttttt cataggtcag ataattatct aagcctacca ctacatagtc
     6001 aggagcctct tcttcccatg taaatccggc agctaaaatc aagtcaatca atcctgcttc
     6061 tccgataaca tagacttttt tcccttttcc atctgctttc ataaaatcga ttgttgccaa
     6121 agtagctgtg tagactgtct ctggagctac atgaatgtca aactcatctg ctagacgatt
     6181 agctactgtt tctggtgact tagtcgtgtt attggtaaca aacaagaaag gtaattttct
     6241 tttttgcaat tcttccacaa atcgtttacc ggccggtatg ggttgtgtcc cacgataaat
     6301 cgttccgtct aaatcaatta aatatccttt atagtccatt catccactac tcttcccttt
     6361 ttctgatcgt aaatgctgtt tttgtttttt ctacttgttt aggttgttct tgttttttta
     6421 ctttttcaga ggtttttgct gactctgttt ttcgattttt aataaccggt tttttgcgat
     6481 tagatgcgac tggtcctttt ttctcatcga tatacgcacg attatggttt tttttctttt
     6541 ttcggcgctg agttgtcttt tcttttttac cgccgattct ctcgatgaca aaatacgcac
     6601 aaccaaaatt gcaatattcg taaagatagt cttccaagga atcgattcgc tgatctggat
     6661 gcgcccgtcg attatccgct tggaaaaatc cttttaggcg tagttgttca taaccccaat
     6721 ctcctacgat ataatcatat cgggctagta catcactgta acgttctcca agcttttctg
     6781 cattaaatcc ttcacgataa tcggacacaa gccgataatg tctttcacct atcaaaaaat
     6841 tactgtcatc taacagtgtc actagctcgc ctttgacttt ttctgcttta ggctcctcaa
     6901 cgatttcttc caaagcagct gtcagttctt cggtgactgt tgtttcctgt tcagctgatg
     6961 attttttctt agcaatttct gttttatgct caacgttctg ctgttcattt tgttttgcag
     7021 acatgcgttc accacctatc ttttttatta taccttgttt ttatttgatt gggtagtgag
     7081 ctttcaagta atctcctacc acacttcgga taaattcagg gaacaaaaat tcattttttc
     7141 cagcaatctc aatcgttgga aaaaatggaa caaacatcaa atgatctacg gtagtaaaag
     7201 tataattttt attcagatcg attggcttgt tcttccaaaa gacttgatgg ttaatagaat
     7261 catatgtgat tccattatag accatctgcc caaaaatctt accgcgaaat cccattccct
     7321 tcataggata gttacgtaaa aagttacgat ttttttctat ttctaaaact aagcggatca
     7381 gatcatttcc acccaatgtg acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa
     7441 gctgatcctg attgacccgc ccttttggaa tttcagttaa aaacagacca ctgtttaata
     7501 ttgctgcttc cgtctttcct cgttctttca ccgcctcaaa ggctacttgg ataaacgaat
     7561 gctcattgaa gatatctaga ctcagagaaa atggcaaatc tgctacttct ttttctttta
     7621 ataattcatg cccacgttct agataactat ttatttcttg ttcatcttct gtaaaagctg
     7681 tcattgtttc ggtagggatg gctttggctg aatgcttaag gatttttttc tcttcatcga
     7741 tggttaaatg gacttcaccg acatactgcc cgtactttcc agcggctgct atttggacat
     7801 cattgattac gtgaccttct ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa
     7861 ttgctggtat ttcttttgca atttggaaat catcctgaat cccgagatga ctcatcaaaa
     7921 ctaaaacatc cacttgtgaa cgtaaatctt cgactaattc tggcaaaata tcataaggat
     7981 tccgtatgtc ccagccattc ggattataag taagcggaaa tggagcagta aatgcaatta
     8041 gtccgatttt cgtctggtgt ttcgttgtaa tgattttata tggtttcgtc catgccggtg
     8101 gctggagcgt atttttatcg aataagttat ccaggatgat atcaaactca gcatgatcgt
     8161 ataaatgatt caactcttct ttggaatttc ctacgccttc attgtttcct atcgtcgctg
     8221 catcataacc aacttcattc atcagcaaga tattttcctg tccattcgtt gcttctgaca
     8281 atggatgcca acgatccaca aaatcaccta gatctactgc aatcgttgtc gttttttctt
     8341 tcttttcgtt ttctcgttta cgttgatcca aaaaccttct gatccttggc caattttcta
     8401 aatgagaatg cagatcattc gtatgtaaaa tcactatttt ttccatgtac cctctcgcct
     8461 ctctttatct ttatcatacc ataggcagct ttttcttcca ttgaaacaag ctctatttca
     8521 gagtttttcc tgccttgaag caaattgtat aagtacaata ttcgataaat cagtctggct
     8581 aaaaaatcag gaacattcat ttttacagta ctataaaaat aaaaaaaaca gacccgaaag
     8641 tctgttgtcg tttcattata atcagacaca tggcggaacc tgcttagtgc tgcttccttc
     8701 cagacctgac acgcttcaca agcccaccat tgtcctagcc tttcggcaaa gaatagtata
     8761 ccttattttt cattcgttga ccagtctttt tttaatttct tttttcgctg gcggagcttt
     8821 cggaaaaagt cagtcaaaat catcccacat tcgtcttcta aaatgccacc ttctacatat
     8881 gcttggtggt taaaccgctc gtcctccaaa agattcatca aggtaccggc tgttccgcct
     8941 tttggatcat acgcgccgaa ataaacttct gctacacgag acaaaatcat cgcgccgctg
     9001 cacataggac aaggctctaa ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc
     9061 actttttcac aggcttcctt gattgcaagc atttctgcat gtgtagtagc atcttgcgtg
     9121 gtttctctaa ggttatatcc tcttccaatc acttttccat ccaatacaac cactgctcca
     9181 atcggaactt catgaagcat ttctgctttc tttgcttctg ccaatgcaag gcgcatccat
     9241 ttttcttttt ctactgcatc aagatttgta tttaccaatt acccttccct gcctttgttt
     9301 tttcttgttt tactttagca tatgataaaa aggctgtaac taactttttt ttctatggta
     9361 aaataaatac aagaggtgaa caagatgaaa atggataaat tacttgctct ttacccacaa
     9421 gcacagaaaa aaaactctcc agcatcagaa gcggatatcc tttcttttgc aattgatgat
     9481 gcttttatct ggatcaaaca agattcatta tctcaacaag aaaccagcct tttgaaagcg
     9541 ttattccctg taatcaacga tcaaaaaaaa cacccatggt atcgctatct ttttaaagat
     9601 gcttcttgtg ttcaagaaaa aagctttcgt ttgatccagc ttcacgtcga atcaaaagga
     9661 gaattcttaa aaaaagaatg gcaagatact attttggaat tatttcgcta ttctgctgat
     9721 ttcttttttt atacagacac agatgcgctc ttagtcgaac aaataaaccg tgattatctt
     9781 gatgctactg aaatcaacgg tatttttcta tctttagatg ctgattttga tacgacaacg
     9841 tctgtattcg ttggttcttt ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa
     9901 gaaagaaaaa tttttttaac agagcaaaac aatctttata ctcacagccg tactttttct
     9961 atgcaagatg tggctttaca ttactataca aaagatactg taaaagatag taagttgatc
    10021 aaaagttaca tagaactgat caaacaaaat gaaatggaag tgatcattca agaattatgg
    10081 aatgaattag gcaatgtcag ctctgctgca aaaacactgt ttcttcatcg taatactcta
    10141 aaatatcgaa tcgaaaaatt tcaagaacag tctggtttca atctaaaaaa agccaacgat
    10201 ttactgtttt gctatctttt attgcttcat gaacataatt aaagatagat taaaggggtt
    10261 gtgacaataa ttttgtcaca accccttatt tcgaatgaat agtgttcaaa agctagtttc
    10321 tcttacatca ttttttcaac aaaatgcatt agccggtctt ttacatcttc ggttgaatct
    10381 ttgattgaat caccataaac agagattttc ttttttcctg atttgatttt tgctaacatt
    10441 cccatcaaag aatccaagtc actatctgaa agaccatcta cgatttctag taatttttca
    10501 tttccatcaa atttgtcata gacgaatttt tttactttcg tccgagtagt catatgatgg
    10561 atcttttcca ttacttttcc cgatacaacg actgctgtcg ctacactggc agcggctgct
    10621 acgcttaatc cgattgttac ttttgttgat gttttcataa gtatcagctc ctttttatct
    10681 atctccatca tagcatagac aaaaaaataa acgaactaat ccactcagtg aaatctcaat
    10741 cctttaggga aaaagttctt tactgttcct tttacttgtt tcccaaatcc gacaggtatc
    10801 ttatctaata ctagcaaaac ccatccttga ttcccatcac gttgaaatgt ttctcctgct
    10861 gtgtaagatt gccattcctt ttgagtgatc ggcaagcaag gtatattctc tatctttttt
    10921 gtagcaagcg ctaatgcata gctaggttca aatcgattct ttttgaaatc tcctaaatgc
    10981 aaacctgtac gtacgacttt taaaccatcg agagaagggg ctaactcagg aacttcccaa
    11041 agatggtcgt tgaacactaa aagtcttcca gtagcttcat aatgaaaatc atttgaaaac
    11101 tccgtccaga gtttttcctg ctcttttgtc atttgcactt tacttttttt tcgtgtcttc
    11161 ttttctttat gcatctgatt ttgtccatga aaagtcagct ttgcaacaaa atgtccttca
    11221 ccctgatctt tatgaggcca tatacgtatt gttttttcca aaccagctac gcttccccat
    11281 tcagaacgtc ctgaactaac ggattgagtc aaaggaatct cctcaatcgt tactggataa
    11341 ttttctacaa gccagctaat gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa
    11401 taaatcagtt gtcctttatt ttttagcatt tttattgctg aagacaaaat ctcttgctga
    11461 cgtttttgac agtatagcgg agattcttct gtccactctt ttattgcatt tggatctttt
    11521 cggaacattc cttctcctga acaaggagca tcaaccacga tccgatcaaa aaagcccgaa
    11581 aaatgaggga caagttctgc gggtgcatga tttgtcacta ttgcgttcga aactccccat
    11641 cgttctatat tttctgataa aatcttcgca cgtttcggaa agatctcatt cgtcactaat
    11701 aaccctttcc ccttcatttg agcagctaat tgggttgatt taccaccggg agccgcacac
    11761 aaatccaaaa ctttttcacc gggttttgct gcagcagctg ttccaacgat cattgcactt
    11821 ggttcttggc tatattcata cccagcttga tggaggaaag actttccatt gacagtgcca
    11881 agaaatccct cattgctgta gggagcgggt tgtaattctt cgctatggta agtttggacc
    11941 atgtccaatc ctgccggttt tagtggattc caccgaaatc cttttttcac gcttccttgt
    12001 tcaagcgcag aaaaaaaatc ggatgcttct tcgcctaaaa gaagacgata ttttttgata
    12061 aattgctgtg gtaacgttgt cgcttcctcc ttcattccaa tgtttcgtta gacattttac
    12121 aatctttttt acgaaaaata aaggtggctg atctactttt tcatgttgct gcctaaggtg
    12181 actgttcctt taagatattt tccaagtatt catccgtcac ctcgttttta tcccaaacag
    12241 cgttcatcat ttcaagaaaa tcttgagaag aatcatctga gcgcaaactt cccaatgcat
    12301 taagccatac caattcaagc gacttttctt ctttgatctt cgtgtgacct ttgacatatg
    12361 agaaaaatgg ccgcttaaat agcgaatcca tgtctaaatc agattttgca atgatttcac
    12421 gaatactttc atggatagaa aaaaaatctt tcaataagta gacaatgctt aaaacatcat
    12481 cggaactgca ttcagctaag tagttttcaa aataaacagt gatttcttct aatatcttct
    12541 cttttaaccc gctgattgat ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga
    12601 actctgctaa ctctcttgca gtaattgact tcattccata ttcttccacg tataccatac
    12661 ttttgaacag caaatattct ttactataat ttgttactct ccccatcatt cttacctccg
    12721 tctcttttca tctacccata aataaataac ataaataatt ctaaaaatat tattga
//
LOCUS       DAZUKZ010000071        12706 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000071 DAZUKZ010000000
VERSION     DAZUKZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.073"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..236)
                     /gene="rpmF"
                     /locus_tag="ACG8EF_001929"
     CDS             complement(57..236)
                     /gene="rpmF"
                     /locus_tag="ACG8EF_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS4947226.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(294..863)
                     /locus_tag="ACG8EF_001930"
     CDS             complement(294..863)
                     /locus_tag="ACG8EF_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFS4947227.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1049..1951
                     /locus_tag="ACG8EF_001931"
     CDS             1049..1951
                     /locus_tag="ACG8EF_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4947228.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(1986..3770)
                     /gene="pyk"
                     /locus_tag="ACG8EF_001932"
     CDS             complement(1986..3770)
                     /gene="pyk"
                     /locus_tag="ACG8EF_001932"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFS4947229.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3844..4806)
                     /gene="pfkA"
                     /locus_tag="ACG8EF_001933"
     CDS             complement(3844..4806)
                     /gene="pfkA"
                     /locus_tag="ACG8EF_001933"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFS4947230.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(4974..8288)
                     /gene="dnaE"
                     /locus_tag="ACG8EF_001934"
     CDS             complement(4974..8288)
                     /gene="dnaE"
                     /locus_tag="ACG8EF_001934"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFS4947231.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8519..8704
                     /locus_tag="ACG8EF_001935"
     CDS             8519..8704
                     /locus_tag="ACG8EF_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFS4947232.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8766..9962)
                     /locus_tag="ACG8EF_001936"
     CDS             complement(8766..9962)
                     /locus_tag="ACG8EF_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS4947233.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10132..11568)
                     /locus_tag="ACG8EF_001937"
     CDS             complement(10132..11568)
                     /locus_tag="ACG8EF_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS4947234.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11861..12670
                     /locus_tag="ACG8EF_001938"
     CDS             11861..12670
                     /locus_tag="ACG8EF_001938"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4947235.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3791 a         2670 c         2248 g         3997 t
ORIGIN      
        1 aaacctcaag gctccatggt tagccttgag gttttacatt ttctaattaa aatgtattaa
       61 gcttcttttg acattacgtc cttaccatca tagtaaccac aatttgcaca tacgtgatgg
      121 ctttttttca tttcaccaca gtttggacat tcgtttaacc ctttgattgt caatttgtag
      181 tgtgtacgac gttttgcttt tttagctttt gatgttcttc tagctagtac tgccattttg
      241 ctacacctcc ttgtaaagtc ttgtatctaa attagacaca atatattcca atcttacttg
      301 ttatcgtcgt cttttgattt agaatcaaaa agttcagata gttttgcaag acgaggatca
      361 attttattct cggcttcttc catttttctg cgctggtatt cttcttcgga aacaacttcc
      421 caatcattgc cttttggaag ttctgttgct gcttcctctt cttttgtcaa tacccgcatt
      481 ggaatcgcca ataagatgtt gtcttcaatc gactctgcta aattcaataa tggaccttca
      541 attagcaaaa tctcatctgc agacatttgt tcatctctac tttggaattg ttcttctgtc
      601 atgaatactt cgtctacaga aaaatccatg ggcagttcaa caggttctag tgaacgtgtt
      661 gaaggaactg tcaaagtcgt ctgcacagtg taatgaacga tatagccttt cttatcaact
      721 gagaccaaac cttcgacttc cactggcgca acatcaagta ttaaattgtc tcgcttcatg
      781 agagcggctt tcaagtcgag cgtctcatga aaagttaacg gtgtctcctg gtattttctc
      841 aattccaata aagaccattt catttttcat cactcctaag caacaaaagc aattatacag
      901 ggcaaaaagc atgttgtcaa tgaaattttc ttgacacctt gcagttcata aaaaaacaga
      961 aaaatcttta ttagaaagcc tctgtttgct atcatcctca gaatcattta caataatcat
     1021 tattccatta aaggagcgga tgatcttcat gaacacgaaa gatatcgaat actatgtcca
     1081 aatcgtcaaa caaaaaaatt ttacgaaagt ggctaattat ttccgtgtga gccaacctac
     1141 tattacatat gcattacaac gtcttgaaaa agagtttgac accgtactta ttacaagaga
     1201 tcgatcgcat cacgaattaa tcgtcacgcc ttctggaaag cagctgtttc tccatgcaca
     1261 aaccattctc caagagattt ctactgcaag agctgaaatc actcagttgc aaaagaaaaa
     1321 gcttcgtttt ggcatgcctc ctattatcgg caatgaatac tttcctaaac tgtcaagtta
     1381 ctttatcaag catgaattga tgaatcaaat tgagattacc gatggaggct ctcgggactt
     1441 atacgggatg atccgacaag ggaggatcga tttagctgta ttaggctcaa cacagccaat
     1501 cgtggatcaa tatttagaaa cagagttact tttggacaaa cgtttcatga tcgtcgtgcc
     1561 acctacacat cctctttcca agcaggatac tgtctctttc aaagatatag cagatgaaca
     1621 gtttgtacta ttgaacgaac attatgtcca ccccgcagca ttcactaagc tttctaaaca
     1681 aacacatact catccaaaag ttgtttatca aagcaatgac ttgacgattt taaagagtat
     1741 ggtccgagaa aatgtgggta tcggtttttt ggcagagatt gctgtccatc ctgaagatca
     1801 tttagtttgc ctccctcttt cagatgaagt tcagcctcgc ttcttgattt cattggcaaa
     1861 acgaacacag cagatcgtca cgcctcttca agaacaagta gtccaagtta tccatgaatt
     1921 cgttacagca caaaaaaaca gctcttcgta aaagaagagc tgtttttatt ttttattaag
     1981 ctaaattaga tagctgttgt ttcgccacgg taaacgatgc cgcgacgtga atcaacggta
     2041 atcaattcac cttctgtaat tgttgttgtt gcgcctttag caccaacgat tacaggaata
     2101 tcttgtgcaa ttcctacaac tgcagcgtga gaagtcaatc cgccttcttc aacgataaca
     2161 gcagctgctt tttcgattgc aggcatatat tctttgtcag ttgatggtac aactagaacc
     2221 attccgtctt ttgctttttt tacagcttct tcagcagatg aagcaataac tgcatttgca
     2281 acgacagatt tttgtccaac gccttgagct tctaataatt tagaaccaat catttgaatt
     2341 ttcatcacgt tagttgttcc gcgttcacca actggtacac cagcagtgat caagattaaa
     2401 tcgccttctg atgcgaaacc taattcaaca gcttttttag ctgctagttc aaacatttca
     2461 tcagttgttg atggtttttc agcaactgtt gggtaaacgc cccagtttaa catcaaacca
     2521 cgttttgtgc gttcgtcaaa agtaacagcc aagatatctg catctggacg atattttgaa
     2581 atcatttttg cagtgtatcc tgattcagtt gcagcaacga ttgttttcac gcctagattt
     2641 tttgcagcac gagctacaga taaaccgatt gtttcagtta catctgtttt atcgaattcg
     2701 ttcaattgga atgtacctaa ttctgataat gcattttcag cttcttggtc gatacgtgcc
     2761 attgttgaaa cagcttcaac tgggtagtcc ccgtttgcag attcaccaga tagcattgtt
     2821 gcatctgtgc catcaaatac agcgttagca acgtcagaag cttccgcacg tgttggacgt
     2881 gggttttctt gcattgattc taacatttgt gtagcagtga taactgggat accagcagca
     2941 ttacattttt tgataatgcg tttttgaacc attggtacta gttcagctgg gatttcaaca
     3001 cccatatctc cgcgggcaat cataacaccg tcagaaactt tgatgatttc gtcaatgttg
     3061 tcgatacctt cttgagattc gattttaggg aagatttgaa catgagtcat gtctttttct
     3121 tctaagattt cgcggatatc tagaacgtct tgtgctttac gtacaaaact tgcagcgata
     3181 aagtcgatat cgttgtctaa accaaaacga atatcgtcag catcttttgg agtaatacct
     3241 ggcaaactaa tagaaacgcc tggtgcattt acccctttac gtgatcctag catacctgca
     3301 tttttaactt ctacgactaa ttcacggttt gcttcgtctt tttcaatgat ttgcatatcg
     3361 atcaaaccat catcaaataa aatgtgtccg ccaacgtgaa catcgtcaaa taatcctggg
     3421 taagtaaccg cgattttttc ttttgaacct acatgttcag gatccataga gatgcgtgtt
     3481 tgatctccta cttcaaattt gatgtatcct gcacgaccaa aatcagcttc tgttgtgcct
     3541 tgaacagttg tacggatttc agctcctttt gtatctaaaa gaataccgac atcttttcct
     3601 gtctttttaa ctgcttcacg gaccatagtc atacgtgata attgttcttc atggtcacca
     3661 tgagaaaaat taaagcggaa gacatttgcc ccagattcaa tcaattgaga aataatttct
     3721 actgagttac ttgctggtcc taatgtacta acgattttgg tttttttcat ttaaaaaact
     3781 ctcccattca attttgttta tttaaagctg tacacagctt aaatagcttg gtttctttca
     3841 ctcttaaaat gaaagtgaat tatttagatc atatagtgat aagtctggtt tatgtttgtt
     3901 gttttcaagt gtatcaatga tgtcagacgc aacgatttcg ttgtcatgga taccgacaca
     3961 taatccacct tttccttctt gaagcaattc gacagcgtaa gcaccgaatt tacttgccaa
     4021 cacgcggtcg cgtgcgcttg gtgctccacc acggacaacg tgtcccaaga tagatacacg
     4081 tgtatgatag tcaccaaatt ctgaaagttt ctcagcgaat tcatttccgc ccattacacc
     4141 ttcagccaag atgatcaagc aatgtttctt cccgcgatcg cggccttctt ggattttttt
     4201 agcaactgaa gccatatcaa aatcatgttc tgggatgatg atttcatccg ctccgccagc
     4261 cacacctgac cataaagcga tgtctcctgc attgcgtccc attacttcga taacgaatgt
     4321 acgaacatga gaagtcgctg tgtcacgaat acggtcaatt gattctagta ctgtattgat
     4381 tgctgtatca aaaccgattg tgaaatcagt cccaggaata tcattatcga ttgttccagg
     4441 aatacctact gctgggaaac cgcgttttgt caaagccatt gcgccatgat aagagccgtc
     4501 tcctccgata accactagac cttcgattcc gaattttttc aattgttcga ttccttttaa
     4561 ttgaccttct tcagttgcaa attctggata acgtgcagaa tacaagaatg taccaccacg
     4621 ttggatcttg tcacctacat ctgccacatc aagacgacga atatctccag caactaaacc
     4681 ggcaaaacca taattgatcc cgtatacttc cagtccttcg taaatacctt tacgaacaac
     4741 ggcacgaaca gctgcattca ttccaggtgc gtctccgcca ctggtcaaaa ttccgatgcg
     4801 tttcataata aacattcacc tcattaaaat actttatcct aagtatgact taggtttaca
     4861 accatttttg cgttaaccac gactaacatt ctaacattat ttttctcact tgtcttgatt
     4921 tacggataaa aaaatgaaaa aaaatgaaag caagtagtat tttttaccgt aagttatttg
     4981 aaaaccacat tatcttcgcc aagcagataa ataagttgcc cctttatact cttgctgtca
     5041 tccacccaat aagcatctga cagaactact tttcgttgcg ttttttcgtg atacataact
     5101 actggtatat atcctgggtg tttctgaaaa atcacttgta attgtttcat tatctcggga
     5161 tcttcccgtt cttctgtaat acgcaaatag caagtttgtg aaggatactg ggcttctaac
     5221 tggttaggat cagcaaagct ttctccaatt agttgcagtt cctgattata tttgctgatt
     5281 tcgctccgcc cttgaacaaa gatcactcga tcaacggcaa acaaattacg atatttacga
     5341 taaagagttg gaaaaactgt gacagaaatt tctccactac tgtcggttcc ttcaacaaag
     5401 gccatcagtt ctcctttttt tgtccgaatt tctctaactt ccttgatcat cactaataaa
     5461 ttgactggtt gattagggac gatatctgta atcagctgaa cttgtttagc taaacgaaga
     5521 cgatcaaacc cttctgcagg atgacctgac aaataaatac ctaagtactc ctcttccagc
     5581 ttcaagcgtt cttctagtgt atagtcagcg acttcttctt cttttaatgc catgattccc
     5641 agcaagtcaa gacttccacc actgtaaacg atattttgga tttttccttc gagatcaagc
     5701 atcaattgtt tcctgttagg agataattca tcaaaaactc ctactgctgt cagtgattgc
     5761 agtaactcta atttcagcca acgcttatcg atccgtatga ggaattgatc cactgactgg
     5821 aacgggccat tttgttttct ttcgtttaat atatcttcta caaaatcacg ccgaatacct
     5881 tttatcgctg tcagtccgaa tctgatttct tccttattga ccaactcaaa actggaatta
     5941 ctttgattaa tggacggcga ggaaagtttg agtttatttt tacgtgcttc tgcaatatat
     6001 tctttcaatt ttgatgtgtt atgccgaaca gaattcatca ggctggtaaa gaaagcacct
     6061 ggatgatggg cttttagata cgccatttgg aatcccacga atgaataagc aaatgcatgt
     6121 gaccgattga atccatagtt tgcaaatttt tcgatatatt catatacggt cgctgctgtt
     6181 ttttccgaat atccctgttg taatgctcct gatacaaaat gcgacttttc ttcatctaat
     6241 acgtcttttt tctttttact cactgcccgt cgcagaatat ctgactgccc taatgtaaat
     6301 ccagccatag tcgaggcgat ctgcatgatt tgttcctgat agacgatcac tccatatgtg
     6361 tttttcaaaa tcgggatcag tgaatcatct gggtagcgaa tggcttcttt tcctttcttc
     6421 ctggcaatga aggtatctat attttgcata gggcctggtc gatacaatgc attgactgcg
     6481 gcaatatctt cgatgttttc tggatctaat cttcgtaaaa cgttccggat accagcagat
     6541 tcaaattgaa atactcctga ggtttcgcct cgttgaaaca agagcagcgt ttttgcatca
     6601 tttagcggga tctcttttaa tcggatctca tacccttcta ctcgcttaac agctttcaaa
     6661 gtaaaatcaa taatcgataa gttgcgtagt cctaaaaagt ccattttcaa aagaccgatt
     6721 gcttctacct cattcatcgt atactgggtc agaaaagctt catcagaacc gcgttggagc
     6781 ggaactagtt caagtagatt cttatcgctg ataactacac ctgctgcgtg agtcgaaaca
     6841 tgtcttggca atccttctaa aatcactgca gttttgaata aaagttggtt tttttcgctt
     6901 gcctgaacta attctactaa ttgatgcgaa gttttataag cttgggtcaa tgtgattttc
     6961 aattgattgg gtattgcttt tgaccagcgg tttgcttcac tttgcgataa accaaacact
     7021 ctagctgcat ctctaagaac catttttgct gccattgtgc caaatgttgc gatttgtgcc
     7081 atatgatatt gtccatactt ttctcgaaca taattcaata cttcttcacg tcgattgtca
     7141 ggaatatcca aatcgatatc tggcatcgta aagcgttcgg ggtttaaaaa gcgttcgaac
     7201 agcaaatcat attctaatgg atccacatct gtgatttcta acacataggc tactaatgaa
     7261 cccgcagcag aaccacgacc tgcaccggta acgattttgt ttctatgagc aaaagccatt
     7321 acatcccata cgatcaagaa ataatcatcg aatcccattt gatggatgac agataactcg
     7381 tagtccaaac gttcttggta ttcctttgta tagtcagtga ttcggcgttc cacttgcgaa
     7441 tagcataact ctcttaaata ttctccagct tgttttccat taggaactgg ataatgcgga
     7501 agtaatttct gatggaactg gaattcaaat gaaatcgatt cagctaaacg ctgggcgcct
     7561 attacagctt gggaaagacc cgctttttcg aatcgtttga tcatttcttg ttctgaaaga
     7621 agatagacat ttgctgttag tggtaaagat gcagataaat tttctaaagg tatttggctt
     7681 ccctctttga tatgtttcat cacttcaagt gcaaaaagtt cttcttgttg taaactagcg
     7741 atgggatgta atgcacaaag cgaataattt tcttttatca catactgata ccattcaggc
     7801 aattgatcag gcgtgttttc tgtcaagccg atataaaaat ttctagtaaa aacattttca
     7861 agtaaaaact gccaatgacc gaaaaagtta tcctctttat tgaaagcctc agaaacagaa
     7921 tgatttgccg gaacaacaaa cgctagatgg tcggtttctg tcatttctgc aagttccaat
     7981 ttttcttgga tcatttttcg gctggaaagt ttcatcagct gctgataacc tatttcatct
     8041 tttgcgtacg caagcaagcc ataatttttt tcttcataag tataactaag atgtaatcca
     8101 atgattggct gtactccttc ttctacacaa atacgataga attcaggtgc accgtataag
     8161 acatctttat ccgttatggc taatgaagtg tagccttgtt tttttgcttc ttgtacatat
     8221 tctcgaatac gtatcgtact ttgtaaaaga gaataagaag taattgtata aagttgtggg
     8281 aaaaacatct tgcattccgc cttttttttc gttaaaattc actagcgtta aaaggtttga
     8341 tccttttact ttcattaaga tagcaggcgc tgaagttgat tattccactt catttgcttg
     8401 cttgtaatct gtaggaatga gttctttcgc aagaactcaa attttattga gatttggtct
     8461 cgtttcttta cattcaaaga aaatctggta aaattaagat agaaaagagg tgttctttat
     8521 gcgatatatc gttacattat tctgggctgt cgttctaggt caagtagtcg gttatattgg
     8581 tgctgcatta acaagcggaa catatgattt tactttgaca acaatcattt cattcattgc
     8641 gggtgtcatt atccttttga ttggtgcagt tgcgccaaga aaagaaacct ctgcccattc
     8701 gtaaaaaaac agccaagagc ttgagatttc tcaagctctt ttttattttc tactcctcac
     8761 caccattata atctgctgcg aagaacttta cgaaagttga ataaagaaag aagaatattt
     8821 gacagaacaa acaaacttgt cacaataaaa acagcactgt aattataagc acgcgcgact
     8881 gtcgatccta gcatgggacc taaaacttgt ccaaagttgc taaacatctg attgtagcta
     8941 tagattcggc tgactccttc ttgaggcgta atcttactga tcagtgtatt gacagaaggc
     9001 atcaaagcgc cagttgaaaa gcctaaaata aagcgaagta tacctaattg gagcggtgta
     9061 gtgacgaatg ccataggtaa ataacagcaa aatgataaaa tgagtcctcc aattagaact
     9121 ttttgattcc caatcttatc ccctagtttt cctagtttcg gtgaagaaaa gacagcggaa
     9181 acaccagcta tcgaaacaat caagccgctg acaaaaagga tattttctgt acttccgctc
     9241 aattcacgaa tatataaggt caaaataggg ctgatcgtag tgattcctat ctgtaaaatc
     9301 aaggtcgtaa tgaagagacc gatcaaaata gagagatgat ccatttttga aaaaatttct
     9361 ttggtgctta tcagatcttt tttctcgatt ggatggaaat cttctttgac taagaaaatg
     9421 gtcaataccg ttgtgatcat caacaaagcc cctgtgatca agaatacatt ttccatacca
     9481 aaccactgag ccagtgcacc gcccattgaa ggtccgatca gatttccagc aatcgctccg
     9541 gtagacaatg ttcctaacgc ccatccgctc ttttctctag gtgcctgtga agcaatcatc
     9601 gctgtggcat ttggaatata tccagacaaa atcccattcc aaaaccgcat gatcaaaagc
     9661 caatacgcgt ttggtacaaa agccaaagat cccattgtta atgtcatccc tgctgctgca
     9721 cggatcatca tgagttttcg gccttttcga tcagctagat ttccccagat tggtgcaacc
     9781 acggcagatg caaaagcagt cacagagatt gctagtcccg aaaacaattc gatttgatct
     9841 tttggtgttc ctagttgctc aacataaact ggtatgaatg gcataactaa actaatactt
     9901 gcaccagtga aaaaacagcc aatccacgca atcactaaat tttttctcca attaatctcc
     9961 atctatcatc cctctttcgt ctcttttatc atacctattt tcgaataaac aagcaacgat
    10021 ctcgccagta aaaagagaaa aatcccgcaa aggtcatttt atatgtaacg ttctttttat
    10081 gtaaaacaaa aagagatcag aacaaatact gttccaatcc cttctcttta gttaaattgt
    10141 tgctagaatg atgaagttga taatgaataa aatatcaacg atccacaaag caacagaaac
    10201 ttgattgtat tttcctttga ttactttcac aattgtatag aaaataaatc ctgctgcaat
    10261 cccataggaa atgctatagc acaatcccat aaagatcgat gcaaagaagg ccggtaaagc
    10321 ttcttccata tctagccatt tgatgtctgc aaaagaagcc atcatcatta ctccaactaa
    10381 aatcaatgct ggcgcagttg cttgatttgg tacaatggcg atcagtggag agaataaact
    10441 acttaatgcg aatagcactg ccactactac tgaagtcaaa ccagtacgtc caccggcccc
    10501 gatcccagct gcagattcga cataagttgt tgtattcgat gtaccaaaga tagcccccac
    10561 agaagtagcg atcgcatcgg caaatagtgc tttatccatt tttgttttga acccacgacc
    10621 atcttccaac gcgatctcgt cttcttttga gaaaatacct gtgcggcgtc ctgtaccgat
    10681 aaatgttccg atcgtatcga aagtatcaga taaactgaat gcgatgatcg tcatcaatac
    10741 ttgaggaatc ttggaagaat cactgaataa agattgcatg ccttcagaac caaatgctgc
    10801 accgaaagtt gttcctaatt ctttaaatga attacctaac gaattcgttt gccaatcaat
    10861 ggaacttaaa tcaacaacac ccataaaaat accaaggact gtggtcgcta cgataccgat
    10921 aagaatagcc ccacggacat tttttacaac taaaatggct gtcagtacca aaccaatcac
    10981 agccaataag ataggagcgt tgtcaaagtt ggctaacgca gggacgattc caccatttat
    11041 cgtaacattt gtcgcttttc cgccttcaac gacagagctc gaaatagctg actggtctgc
    11101 tgaaaaagat aagaatcctg catttttgat tccgacatac gcaacgaaaa taccgatccc
    11161 gccaccaatc gcatgttgaa gactttcggg aatcgcccga ataatcattt ttcgaatatt
    11221 tgtgacagta ataaaaatat tgattaatcc gcaaataaac accattgcca aggcttgttg
    11281 ccaagaataa ccaagcccaa aaacgaccgt aaaagtgaaa aatgcgttca atcccatacc
    11341 aggtgcctga gcatatggta catttgcgaa caatcccatt accagcgtac caataatcga
    11401 tgcaatgatt gtcgctagaa atactgcttg gaagggcata cctgatgctg acaaaatcgt
    11461 cggatttacg aataagatat aactcatggc aaagaatgtc gttactcccg ccattatttc
    11521 agttgataca tttgtattgt ttttctttaa accgaaaaac ttttccataa aaagttccac
    11581 tccctgaatt tttttctgat ggaaaactat aataataaaa aataagtaaa atagggcaaa
    11641 tccatcgaac aatttcaaat tatatagacg tttcattcgc ttttcaacgt ttttttaaca
    11701 aaaaaagcga aaataatact taacgttcgt gtttagctta tgtttagcta tttatatagc
    11761 cataactgta gtttttgtta ggaatagctg attgctcttg ttaattacct aaaatatgct
    11821 agactaaaac aaatgctagt tgaggaagga aggaccctta ttgaataaaa aattaattgc
    11881 catcgatctg gacggaacta ccctcaatca agattcgatg attacatcta aaacaaaaga
    11941 gactctaaaa aaagcaatca atgccggtca tcatgtcagt attgctacag gacgcccatt
    12001 ccgaatgagc catcagtttt accaacaatt agaattaaca acaccgatgg ttaattttaa
    12061 cggcgcatta gtacacatcc ctaatcagca ctgggatggt gaaagagaaa cgttgatcaa
    12121 tcgtgaaatc gcttttgaaa tcttatcgca aaaaaagcag ctaaatttag attttatcgc
    12181 tgctgaaaat cgtgatactt tttttattga cagttttgat ttctttgatg aaaaaatttt
    12241 tgcttcttct cgtccaggag agaagaattt gctttctcct aaaaatttga cgacaaatcc
    12301 tacatctttg cttgtccgaa cagacaaacg ttttgcagga gctgtatctg ctgaattaac
    12361 acgtcaattt ggcagttatg tcgatgttcg gacatggggt ggacctactg ccatcttaga
    12421 gattgtttca aaaggaatcc aaaaagcaaa aggcgtgcaa gagattgcta actacctgtc
    12481 tatcgatcaa aaagacgtca ttgcctttgg tgacgagcat aatgatttgg aattgttgga
    12541 ttatgcaggt tggggagtag ctatggctaa cggtactgac caactcaaag gcattgcaaa
    12601 tgatgtcact cctttgagca atcaggaaga cggtttagct gtttatctgg aaaaactact
    12661 taaactataa tagagaatta gattccacaa aaaagaggtt gtgaca
//
LOCUS       DAZUKZ010000072        12205 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000072 DAZUKZ010000000
VERSION     DAZUKZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.074"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..216)
                     /locus_tag="ACG8EF_001939"
     CDS             complement(37..216)
                     /locus_tag="ACG8EF_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947236.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(232..432)
                     /locus_tag="ACG8EF_001940"
     CDS             complement(232..432)
                     /locus_tag="ACG8EF_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947237.1"
                     /translation="MNKIIPLLFVLFVGEILMRHGAALHLNFVVFLGILVFCLIFWRF
                     VLTLVSLIFKLIAAIFLIGLFR"
     gene            complement(597..1304)
                     /locus_tag="ACG8EF_001941"
     CDS             complement(597..1304)
                     /locus_tag="ACG8EF_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947238.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="ACG8EF_001942"
     CDS             complement(1297..1734)
                     /locus_tag="ACG8EF_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947239.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="ACG8EF_001943"
     CDS             complement(1749..2000)
                     /locus_tag="ACG8EF_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947240.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="ACG8EF_001944"
     CDS             complement(2000..2206)
                     /locus_tag="ACG8EF_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFS4947241.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="ACG8EF_001945"
     CDS             complement(2643..2903)
                     /locus_tag="ACG8EF_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947242.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3077..3181
                     /locus_tag="ACG8EF_001946"
     CDS             3077..3181
                     /locus_tag="ACG8EF_001946"
                     /inference="COORDINATES: protein motif:HMM:NF028245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFS4947243.1"
                     /translation="MPVETALGLMISFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="ACG8EF_001947"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="ACG8EF_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4947244.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="ACG8EF_001948"
     CDS             complement(3810..4709)
                     /locus_tag="ACG8EF_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS4947245.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="ACG8EF_001949"
     CDS             complement(4722..5315)
                     /locus_tag="ACG8EF_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFS4947246.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5391..5540)
                     /locus_tag="ACG8EF_001950"
     CDS             complement(5391..5540)
                     /locus_tag="ACG8EF_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947247.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5556..7760)
                     /locus_tag="ACG8EF_001951"
     CDS             complement(5556..7760)
                     /locus_tag="ACG8EF_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFS4947248.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="ACG8EF_001952"
     CDS             complement(7771..9231)
                     /locus_tag="ACG8EF_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFS4947249.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="ACG8EF_001953"
     CDS             complement(9292..11664)
                     /locus_tag="ACG8EF_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFS4947250.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            12074..>12205
                     /locus_tag="ACG8EF_001954"
     CDS             12074..>12205
                     /locus_tag="ACG8EF_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS4947251.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT         3040 a         2531 c         2053 g         4581 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtccgt ttcctcctcg taaaatatca tttgtctaat
    11701 agtttttatt caaaatgttt ctagattata aaatttattg aataacacat tcctaacgga
    11761 atttgtttcg atattgtgtg atttctcaga gtacaatgga tgtatggaaa ggatccaacg
    11821 ggaatttgct atttcctcct gtagcgtttg tctaccctgt ttagtctgtt acccgaacca
    11881 ctgttatcac taccaactca ttagctgttt cgataatgga ctagactttc aaatagtagt
    11941 ttggaaggca gcttctagct cttttatcac cgtattctat taggcgaggt tgtggctata
    12001 cagtgagttt ctgggaaatc ccacagtatc gaagcatttt ccaaaattta tatcgaaagg
    12061 aggtccttct tccatgaaat tatttgtcgg tattgacgtt agctctgaaa aactagatgt
    12121 ttgtttttta acagacgaca atcaattgtc tatcttatct gaaatctctg ttgccaatga
    12181 catcgaaggt gcttcattca tacga
//
LOCUS       DAZUKZ010000073        12180 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000073 DAZUKZ010000000
VERSION     DAZUKZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.075"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(142..306)
                     /locus_tag="ACG8EF_001955"
     CDS             complement(142..306)
                     /locus_tag="ACG8EF_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947252.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            601..1929
                     /locus_tag="ACG8EF_001956"
     CDS             601..1929
                     /locus_tag="ACG8EF_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFS4947253.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2131..2964
                     /locus_tag="ACG8EF_001957"
     CDS             2131..2964
                     /locus_tag="ACG8EF_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFS4947254.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2980..3717
                     /locus_tag="ACG8EF_001958"
     CDS             2980..3717
                     /locus_tag="ACG8EF_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFS4947255.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3872..4840)
                     /locus_tag="ACG8EF_001959"
     CDS             complement(3872..4840)
                     /locus_tag="ACG8EF_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFS4947256.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5072..5905
                     /locus_tag="ACG8EF_001960"
     CDS             5072..5905
                     /locus_tag="ACG8EF_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFS4947257.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5939..6778
                     /locus_tag="ACG8EF_001961"
     CDS             5939..6778
                     /locus_tag="ACG8EF_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4947258.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6804..7364
                     /locus_tag="ACG8EF_001962"
     CDS             6804..7364
                     /locus_tag="ACG8EF_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4947259.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7563..9284
                     /locus_tag="ACG8EF_001963"
     CDS             7563..9284
                     /locus_tag="ACG8EF_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFS4947260.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9449..10591
                     /locus_tag="ACG8EF_001964"
     CDS             9449..10591
                     /locus_tag="ACG8EF_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS4947261.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10607..11818
                     /gene="thiI"
                     /locus_tag="ACG8EF_001965"
     CDS             10607..11818
                     /gene="thiI"
                     /locus_tag="ACG8EF_001965"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFS4947262.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4152 a         2042 c         2530 g         3456 t
ORIGIN      
        1 ggagtaaata aagcttatgc aaagaaaaag aaatccaatc acttgggttt ctttttcttt
       61 tattattatg atgaatcaag ctttctgttt ttgtcacgta tgttgtaatc tttacgaaag
      121 agtagagttg gctactcata atcaaccata cttcccatca tcgctaaata cgaaataaat
      181 cagcattcct acaccaatca aagcacaagc tgcaccaaga aaagggatga cattcccaaa
      241 gttatctgta ttcataagta tgccaccacc cacaaacaaa aagccgccag caatcaggtt
      301 tttcatttta ttcatctcct ttcttacgtt atagtatttc cttctttcaa tgagtatttt
      361 gaaagctatc ttattgtatg atgttcttct aggcctcttc tttattatac aggccatttt
      421 cgttatctca tagaaatatt cctttttatc tgaaaactat tgtataccgt atgacatttt
      481 tcttcatgaa gttgtaagta tattctcatt caaatgaaaa tttcatcata gaagagattg
      541 tatttctaaa ttcttaagag taaaatacga agtataactt ggatttggaa ggatgattgg
      601 atgaaagcta ttacgcctga tatgacaaca aaatttaaca aacagtttca agcaaatatc
      661 aaacagcagg cattgcagcg ggcagttgtg aagaacggaa ttactgcttc tgctgaaaat
      721 gtttcagcac atgtggagaa tacaccggta ttttctatag atttggctac ggggaaagtg
      781 gctaatcaaa tgcaatcggg gcgttgctgg atgtttgctg cactgaacac attccgtcat
      841 aagatgctga atcaattcca tttaaaagag tttgaattat cacaaaatta cacttttttc
      901 tgggataaat atgaaaaggc aaattatttc tatgaaaaca tcattgcaac aggaaaagag
      961 gcaatcgata gccggaaagt agctttctta ctcgctactc ctcaacaaga tggcggccaa
     1021 tgggatatga tcgtttcatt attccaaaaa tatggcgttg tgcctaaatc ggtgatgccg
     1081 gaaagtgcga atagttcaaa ttctcgagat ttgaataact acttgaacaa attactgcgt
     1141 aaagatgcag tagtgttacg gaaaatggtt gctcaaggag aaacattaga cgctattgaa
     1201 gaaaagaaag aagaaatgct agaaagcatt tataacttct tagctatctc attaggaaca
     1261 cctccaaaag aattcgattt tgagtaccgc gatgaagaaa agaactatca tcttgaccga
     1321 ggactgacac cacaaatttt ttatgataaa tacatcggtg taaaattaga tgattatgtt
     1381 agcgtgatca atgcaccaac aaaagacaaa ccattcaatc gttcttatac agtagagatg
     1441 ctaggaaatg tcgttggcgg gaaagaagtc aaatatctga atgttgatat ggaaactttc
     1501 aaaaaattag cgattgctca gcttgaagaa ggagaatctg tttggtttgg ttgtgatgta
     1561 ggtcaatctt ctactagaac tagtggcatc atggccttag atgcttattc aatggatgat
     1621 ctatttgata cggattttac aatgactaaa gctgaacgtt tagattttgg agaaagtctg
     1681 atgacccatg cgatggtatt gaccggtgtt gacttagtag acgggcaatc aacaaaatgg
     1741 aaagtcgaaa atagctgggg cgaaaaagtt ggaaagaatg gcttctttgt tatgagtgat
     1801 gcctggatgg acgaatatac gtaccaaatc gttgtaagaa aagatttatt gacgaaagag
     1861 caattaaatg cgttcaatga agaaccaatt gttctatctc cgtgggatcc aatgggtgct
     1921 ttagcataga cgagtatttc aatgaagagt atgtagtaaa gataaaaaga gctggcttaa
     1981 gccagctctt tttatcttta aagtccattc cctgtttttt atggtccctg tgctataatt
     2041 ctaaataaag gataaggatt ggaaataatc cttttatcat taaaaatatg atgacgaagt
     2101 agaataaaat tgggagtagg ggggaaagtc atggatatcc aaactgtttt atctgatctg
     2161 aaattaaatg gtaaagtgat tccagttgtt ggtggcgatg ttaatcagac ctatcgtata
     2221 aaaaccgaac atcgtgctta ttttttaaaa atccatccga atgtaaaaaa aggatttttt
     2281 gaagcagaag tagacggatt aaaagaactg tcagcattcg tacgtgtacc ggatacgtat
     2341 atgctaggtg aaacaagtga aggcgcttat ttgttaatgg aatggatcga gccgggaaaa
     2401 ggggatcaac gagatcttgc tgcagcttta gctaatctcc atcagcaaac agcgccacaa
     2461 tttggttttc gtaaagataa ctatttgggt acacttgttc agaaaaattc ttttgaagaa
     2521 gattggtgga cttttttctt taaagatcgg cttgaatccc agatttcttt ggcagaagaa
     2581 acgaatcgat ggaatgtaca gcgtcaagaa aagtatctgc gatttaaaga acgtgtattg
     2641 aaatcggttg aaccaaaaaa gatcactcca cgcctcttgc acggagacct gtggagtggt
     2701 aatgtgttct ttgaccagca agggcatcca gtttttgtcg acccagctgt ttcttatggg
     2761 aatcgggaac aagatattgc aatgagccag ttattcggtg gtttccgacc ggaattttta
     2821 gatgcttatc aaactatatt tccattagaa aagggatgga aagaccgact gccgatttac
     2881 cagttatatt atcttttggc acatttgaat atgtttggag aaagttacgg ttcccaagta
     2941 gatcaattac tagaaaattt ttaagaggag ggcgtgtttt tggcaaaaaa atataccaaa
     3001 aagcatgaag tttcatatta tgagtgtgac atcaaccaaa cgatgacatt tccttcgatg
     3061 ttaggagtag ttatcaaaac gtctgaagat caaagcgacc agttgggaag aggcagtgaa
     3121 ttcgtcaata gctttggact tacttgggtc atcacgaatt attccatgga gattaatcgt
     3181 ctccctagag tcggtgaagt aatctcagta acgactcaag ccatggagta caataagttt
     3241 ttctgttatc ggaacttctg gatccatgat gaagatggga aagaattagt caaaatagaa
     3301 tctgttttcg tattgatgga ttttgtgaat cgaaaaatga gcagtgttaa tgaagagatc
     3361 attgctcctt ttgagagtga gaagatcaag aaaatcaagc gtcaggaaaa aattgaaaag
     3421 attgattcgg gcttcatgtt gccatatcgt gttcgttttt atgatatcga tagtaatcag
     3481 catgttaata atgcgatgta ttttaattgg atcattgacg tattaggata tgactttttg
     3541 actacgcatg ttccagaaaa agtaattatt cgatttgata aagaagtaga gtacgggaat
     3601 gagatcgaaa gccattttga acaaatccct atcgaaaatg gtgtcaaaac aaaacatgaa
     3661 atccgaatgc aagaacagct gtattgtgaa gcaaatatta tatggaaaaa taaataaaaa
     3721 agaggttgtg acagaagggg gcaacttcaa gaaataagaa gaaatttacg aaaattgttt
     3781 ctcaaatttt tgtaaatttt cgacttagtt ttcgaagaag ctatttttgt cacagcctct
     3841 ctttttaagt ttagctatta gataatgatg tttagttttg gataaacgaa atcaattctt
     3901 cacccgacaa accatagttt acagcaatca atagttttaa acgtgctttt tgactattaa
     3961 tactgttaca gaaaataatc cccatttttt ctagttcttt tcccccgcct tcataatcat
     4021 aaacaggctc tgcgattcca ttgaaacaac gagaaacaag tactacaggg attttttgga
     4081 caagaagctt ttccaaagca gctaaggctt gtggagggag attccccgca cctaaagctt
     4141 cgattaccaa accgtctact ttcgtatggg cgatggcttc caataagtcc ccttgcatcc
     4201 cagcatatgc tttaacgatc gggatagtcc catctacagc ggaaatgtcc agtcgctttg
     4261 tttctaaaag ttcctgcaag aataaaattc gattcttagt tactaagccg atgggaccca
     4321 atgtaggtgt tcggaaagta gcaacatttg tggtatgggt ctttgtcacg tagcgtgcag
     4381 aatggatctc atcgttcatg accaccaaaa cccccttatc tagcgcttct tcacaacttg
     4441 cgacacgaat cgcactttca aaattataaa ggccgtcact tccaagttca ttacttgaac
     4501 gcattgcccc agtcaaaaca attggtaatt tgttgccaat cgttgtatca agaaaaaaag
     4561 cagtttcttc taatgtatct gtaccgtgtg tgatcaccac tccggatata cctgatgcat
     4621 aagcggacaa gatacgttct tttaatacca acatgtgaga aggcatgatg tgtggtgagg
     4681 gaagattgaa gatatcttca actaataatt ctacatgctt ggggattgtg atttctgcat
     4741 ctaataatgg gtttgccaca tcaggactaa caccgcccgt gagcatgttt tctttcatgg
     4801 cgatcgttcc gccggtgtgt agtacaagta tttttttcat tttacagact tccttacgaa
     4861 taattttgca tagccgttaa atcttattgt atactatata aaagagtttt gctatcagtg
     4921 aagaataacg aatagcctaa cttatcataa aaaagctgac cttattaaaa tggaaaagac
     4981 tcaaccttga cctcctaata caatggagta tctggttgag tttcttttac aagaaagcag
     5041 ccaaggaaat caatcacgaa ggtggccgga aatgatcaga atcggattaa catcgtttaa
     5101 agaacatgac aaattaactg gtaaaaagaa taacactctt tatgaatatg cagggtttct
     5161 acccttagtg gaattagata ctgcctatta tggaattccc cgaaagtcct cagttgagaa
     5221 ttggataagt gaagtacctg aatcatttcg ctttgtagtc aaagtctatc aaggaatcag
     5281 cggtcaagga gactggaagc cttattatca gaccgaagaa gagatgattg cgcatttttt
     5341 agatgttatg tcgccaatgg tagatgctgg aaagatgttt tgctttttag tccaattccc
     5401 ctcatcattc aaatgtacga aagaaaatgt ccagtacttg cgcaaactta gaaagtggtt
     5461 tgagggttat cctgtttcta tagaattgcg ggattattct tggtacagcg atgaatttct
     5521 tgaacgtaca agaaacttta tgtctagagc cgctttttct ttagccatca tcgatgagcc
     5581 acagttgctg aatacaacga ttccttttga tccatttgtc accaatccga actttgctct
     5641 ttttcgattc catggtagga atgcgcaagg atggcaagca aatgataaag agtggcgtaa
     5701 aaaaagaacg ttatatcgtt attcagaaaa agaactgcaa gaactagcac atgaagtcaa
     5761 agtagttgat tctaagacaa aagaggtagg tgtgattttt aataataatt ctggtggtga
     5821 tgcggctgaa aatgcattag cactgcaaaa aatattaaat atcaagccag atggactcaa
     5881 tccaacccaa atggatctgt tttaagggct tattttattc aagaaagggg cgaagtctat
     5941 gatcaaagct attttttttg atattgacgg aacattggtc accaatcgtt caaaagcgtt
     6001 ggaatcaaca aaacaagcaa ttgaatatgc tcggaaaaac ggcattttat gcggtgtagc
     6061 aacggggcgc agcccagtaa agatcaaaga aatcattgat gaattggaat tagatatgta
     6121 tgtcgtgtat aatggtcaac tggtttttac tgcagatcgc acgattatcg accacccttt
     6181 tgaacaaaaa gtattggaac atattgttga atttgcagat gagaaccatc gacaaatcgt
     6241 ttttggtgcg agaaatcggt tagatggatc aacgacgatg ctattaggac aatcgatttt
     6301 tatcaagcga ctagtcagct ttttaccgcg taaattcccg gtacgtctga tgaaaaaaat
     6361 cctccaggtt tttagccctc accgacaaaa ggatcgttat gaaacgttag agatattaaa
     6421 agaaccaatt tatcaatgta tattgctcag tccggagtca gaacagaaaa agttagaaca
     6481 tcgctttccc gagtgtacgt ttcagcgttc aaatacgtat actgtagata tcatcccaaa
     6541 aggtggttca aaattactag gcatccaagc tttcgcaaat gctgtcgaaa ttgaaatgga
     6601 agaaattatg gcattcggcg atcattacaa tgatatcgag atgctgaaag gtgtaggaat
     6661 tggagtggcc atggggaatg ctcagatcga agtaaaacat gctgcagact ttgtgactca
     6721 atcaaatgaa gaagatggga tatacgaagg actgaaacac ttcggtctga tttattagtc
     6781 aatgaaagaa ggaaaaaatc agtatggaag cagagacacc ttttcaaaaa gtagaaacat
     6841 tccacaaaac ttttgatcca agaagaccag agatacccac ggcttttaca gaaaatgaag
     6901 ctcatacacg tgctgcgttc aaaatagaag aacttgttga atttgttgca gctacttcac
     6961 ctaataaaga acaactgcat gaagctgtta tgctgctgca cgaagcatta gataaagcag
     7021 aagaaaaaat acttggtaaa acacagtggg gaagtgatcc gctggttgaa caagtagatg
     7081 cactgacaga tatcctttac tttacttatg gttctttttc attactagga atagtgcctg
     7141 atcgaatatt cgaaatcgtc catcaggcaa atatggggaa attatttcca gatggaaaac
     7201 ctcattatga ccctgtaaca aataaagtgt tgaaacctga aaactggcag atggaatatg
     7261 ctccagaacc taaaatcgcg gcagaaatcc aacgtcagat tcaagaagcg attgctaaga
     7321 agaaatccgc tgatataggc atgtgcacaa atgaaaaaga gtgatcagag tttcactatt
     7381 tttgttatct ttatacgatg gaaaaacaaa agattttcgt ttaagttagt gaatgcatga
     7441 aaatagtatg ctataatcta gaatagatgt cttttgcacg agagacgtat tatttggatt
     7501 tatgccaagc agaacgccat ctgttgcata aaagatgcat actgaaaacg gaggaaccat
     7561 aaatgggaaa taatgttatc atcggaatcg tcattgcaat cattatagtc gccattgtcg
     7621 tctttttggt tagttacttg atgaagaaaa aaaaccaaga tcgattggat gccttagaaa
     7681 aacgcaagga agaattattt gacttgccag tgattgaaga agtcgatgat gttaaaaaaa
     7741 tgcatctggt tgggcaaagc caaaatacct ttcgtgaatg gagtcaaaaa tggaatgaca
     7801 tatctacttc ctcgttcgct gaactagaaa gtcaaatttt cgaagtggaa aacctcaatg
     7861 aaagttttcg gttcttaaaa ggaaaaaaag cagttgttga agcagaagct acaatggacc
     7921 aaatggagaa acaagtgacg gaaatccgtg aaggtctaaa ggaattgcgg gaaacggaag
     7981 aacgcaactc actagctgtt cagcaggcac ttgacgtcta tgaagaactg aaagaaaaag
     8041 tcagagaaga cgagcatgaa tacggaccag ctctttttga attgcaaaaa cagatcaaag
     8101 tgatcgaaaa agaatttacg cagtttgtga ctttaaacac ttctggagac cctgttgaag
     8161 cccgcgaggt tttggataaa gcagaaaaac atacgtatga agtggaagct ctgatgaaaa
     8221 aaattccacc gctgtatgag gagctccata ccactttccc tgaacaactt gaagaaatca
     8281 gtgatactta cgagaaattg atggaagaac aatatgtatt cccagaagaa gatcttgctg
     8341 aagatatcgc aaaagtaagt cgaagaattg aaaattcatt agccaatctt gaaaaaacag
     8401 aagttgaaac ggtcgaattt gaaaaccgtg aaacagctga tttgattgat tcgttatatg
     8461 atatcttaga acgggaaatg gaagctcaga gatatgtaaa aactaaccaa tcaacgattg
     8521 cagagtatat caaacataca acaaagaata accgtcaatt attgattgag ctggatcaca
     8581 catctcaaag ctatacattg aaccacaatg aaattggccg tgtacgaggt ttccaaaccg
     8641 aagtggaaga aatggaacgt caaaatgaac agatgattcc acagatccgt caacacgaaa
     8701 ttccttattc agaagtgcgc acgttctata aaacagtctt caaagtctta gaagatatcg
     8761 aaaagcaaca agtagagatc gacgactcat tacatgaatt gagaaaaggc gaaaaagaag
     8821 cgcaagaaaa gatcgataca ttcgaattca aattgcgcag tttgaaacgt tttgtagaaa
     8881 aacaacgttt gcctggcttg ccgaatgatt acctagaatt cttttttgtt gctactgacc
     8941 gtattgaaga actaagtgtt gtcttgaata aaattcgagt aaacatggaa gaggtcaatc
     9001 gtttagttgc tctatgtgaa gaagatctag aattattaga taagaagaca catgatttag
     9061 tagatgcagc agcattaacg gagcaaatgc tccaatatgc aaatcgttat cgtcatacac
     9121 acgaagaagt gagagaagca attgataaaa gctactattt attcaataaa gaatatcact
     9181 atcaagaagc attagacgaa atcggtacag cattagagcg agtagaacct ggtgctttca
     9241 aacgcatcga agatttttat tttaatcatc cagacttagt ataaagcaaa aaagttagta
     9301 aaccagcaag agcaagacgg attattgtct tgttcttgtc tttttttgta tatttgaaaa
     9361 ttgaaacaag tgaaaatttc acctgctctt gaaagcacct tggacaaccg ttataataga
     9421 aaagaatttc aaaaaagagg ggtttcttat gatatatttt gataacagtg caacaacccc
     9481 aatctttccg caaagcttag atgcatatgt aaaaaccagt cagcggatta ttgggaatcc
     9541 atcaagcttg catgatttag gcaatcaagc taaccggttg ttgcagcaag caagaaagca
     9601 gatcgctgat ttgatccatg tagaaccaga agaaatctat tttacttcag gcggaacaga
     9661 aggagataac tgggtattaa aaggaacgat gatcgaaaaa agagaatatg ggaatcatat
     9721 gatcatttca ggagtcgaac atccggccgt cagcgagaca gcagaacaat taaaagaatt
     9781 aggaatcgaa gtaagtatcg ccccagtgga taaacgcgga tttgtacgtg tcgacgaatt
     9841 gaaagaatta ataagaaaag agacagtatt agtctctgtt atggctgtga ataatgaagt
     9901 aggatcgatt cagcccatcc aggaaatcag tgatcttttg gatgattttc ctaaaattca
     9961 tttccatgta gatgctgttc aggcgattgg aaaagtacca ttctcgcaat ggttgacaga
    10021 aagagttgat tttgcaacat tttcagctca taaattccat ggcccacggg gaactggttt
    10081 catttattgg aaaaagggtc gtcgtttagc accattactg acaggtggag ggcaagaaag
    10141 aaatcagcga agtgggacag aaaatgttcc aggtattgtg gctatggtta aagctttacg
    10201 tttatcatta gagataaagg aacaaaaacc tgagcagaca caacgcttaa aagtagctct
    10261 aatcaatgcg ttgaagacgt atgagaaagt gacgattttt tcagaaggaa ctgactatgc
    10321 accgcatatt ttatgttttg ctctaaaagg aattcgcggc gaagttctcg tccatgcctt
    10381 agaagaaaaa caaatatttg tttctactac tagtgcctgc tcctcacgta aacatatggc
    10441 aagcagtaca ttgcacgcaa tgcatgtacc aaatgatctg gctacatctg ctgttcgaat
    10501 cagcttagat gaaaacaatt cgcttgcaga agtcgagcaa tttatgatta ttttcaagca
    10561 gctttataag aaattttctg caatcaatta aaagaggagt gttcttgtga aatacagtga
    10621 aattatggtc cgttatgggg aactttcgac aaaaggaaag aatcgaaaaa gctttatcat
    10681 gcaactagca caaaatgtca gacaagcttt gaaagacttt ccagaaatca aaatccatgc
    10741 tgatcgggac cggatgcatc ttttattgaa tggcgcagac gggcagctag ttattccaaa
    10801 actagcaaaa atatttggga tccaaaattt ctctccaagt attcgtgtag aaaaagatgt
    10861 cgaagttttg aaacaagctg ttcaagacat catgaaagaa atttatactg ggaaagaaac
    10921 atttaaaatt atcgctaaaa gaagcgatca ccaatttgaa ttgacaagta atgaattgaa
    10981 tcaaacatta ggtaatgctg tttttgatat tttcccgcat atccaagttc aaatgcgtca
    11041 accagatatt ccattacgtg tagagattcg gcgtgatggc gcttatctat cgtatgaaac
    11101 gatcaaggga gctggaggat tgcccgtagg aacaagcgga aaaggaatgc ttatgctttc
    11161 tggggggatt gattctcctg tagcagggta ctttgccatg aagcggggag tggaaattga
    11221 agctgttcat tttgctagtc cgccgtatac aagtgaacaa gcactgcaaa aagcaaaaga
    11281 tctaacagcc aaactagcac cttatgtggg aacgatccat tttatcgaag tgccatttac
    11341 cgagattcaa gaagaaatca aaaagtcagt gccacaagga tattggatga caattactcg
    11401 tcgaatgatg ttgcgtttga ccgatgaaat tcgccgtatt cgtcatggtt tagtcatttt
    11461 gaatggtgaa tcattaggtc aagtggcttc acaaactttg caaagtatgg tggctatcaa
    11521 tgaagtaacg aatacgccaa tcattcggcc tgtagcgaca atggataaac ttgaaatcat
    11581 tgaagtagca gaacagatcg atacatttaa tctttctatc cagccttttg aagattgctg
    11641 tacgattttt gctcctccac agccaaaaac acgccctcaa ctggaaaagg tccatcaata
    11701 tgaagagcgt ttagcaatag atgaaatgat ccaacgagca ctagctggct tgaagatgga
    11761 agagatacaa gcagaatcgt ctaatgaaaa aactgaagaa ttttcagaat tgctataaga
    11821 ggttatgaaa atagtgtttt gaccagagta ttagagaaac aaaacgaaaa aatagctcca
    11881 tgtattttac cgtttttgaa ctaatgtcga agattagctg attgaatacc atttatcaag
    11941 aagttatgga aaagccggtt cttttttact aactgaaaaa taaaatttta tttagagatc
    12001 aagctacagt tgataaatga tataaaaaag agaatttccc atgaaatagc gaggttgtga
    12061 cagaagtggg cagcttcaag aaataagaag gaatttacga aaattgcttt tcaaattttt
    12121 gtgaaatttc ggcttatttt tcgaagaagt tacttctgga acaccgtgta tcagaggttg
//
LOCUS       DAZUKZ010000074        12129 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000074 DAZUKZ010000000
VERSION     DAZUKZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.076"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..564)
                     /gene="frr"
                     /locus_tag="ACG8EF_001966"
     CDS             complement(7..564)
                     /gene="frr"
                     /locus_tag="ACG8EF_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFS4947263.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(567..1289)
                     /gene="pyrH"
                     /locus_tag="ACG8EF_001967"
     CDS             complement(567..1289)
                     /gene="pyrH"
                     /locus_tag="ACG8EF_001967"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFS4947264.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1425..2306)
                     /gene="tsf"
                     /locus_tag="ACG8EF_001968"
     CDS             complement(1425..2306)
                     /gene="tsf"
                     /locus_tag="ACG8EF_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFS4947265.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2405..3187)
                     /gene="rpsB"
                     /locus_tag="ACG8EF_001969"
     CDS             complement(2405..3187)
                     /gene="rpsB"
                     /locus_tag="ACG8EF_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFS4947266.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3546..4025
                     /locus_tag="ACG8EF_001970"
     CDS             3546..4025
                     /locus_tag="ACG8EF_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4947267.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4242..5333
                     /gene="phnW"
                     /locus_tag="ACG8EF_001971"
     CDS             4242..5333
                     /gene="phnW"
                     /locus_tag="ACG8EF_001971"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFS4947268.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5326..5454
                     /locus_tag="ACG8EF_001972"
     CDS             5326..5454
                     /locus_tag="ACG8EF_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFS4947269.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5458..6003
                     /locus_tag="ACG8EF_001973"
     CDS             5458..6003
                     /locus_tag="ACG8EF_001973"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFS4947270.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6457..8148)
                     /gene="argS"
                     /locus_tag="ACG8EF_001974"
     CDS             complement(6457..8148)
                     /gene="argS"
                     /locus_tag="ACG8EF_001974"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFS4947271.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8568..9515)
                     /gene="arcC"
                     /locus_tag="ACG8EF_001975"
     CDS             complement(8568..9515)
                     /gene="arcC"
                     /locus_tag="ACG8EF_001975"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS4947272.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9630..10649)
                     /gene="argF"
                     /locus_tag="ACG8EF_001976"
     CDS             complement(9630..10649)
                     /gene="argF"
                     /locus_tag="ACG8EF_001976"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFS4947273.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10740..11969)
                     /gene="arcA"
                     /locus_tag="ACG8EF_001977"
     CDS             complement(10740..11969)
                     /gene="arcA"
                     /locus_tag="ACG8EF_001977"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFS4947274.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3313 a         2519 c         2192 g         4105 t
ORIGIN      
        1 attttattaa acttccaata attctttttc tttatcagca gcgatcttat ctacttcttt
       61 gatgctgtta tctgtcaatg tttgtacgtc tttttctaat ccgcgcaaat catcttcggt
      121 aatatcacca tttttttgtt gttttttata ttcatcgatc gcatcacgac ggatgttacg
      181 aatcgcgatt tttgcatttt ctgcttcttt tttaacatct ttagctaatt cttttctgcg
      241 ttcttctgtc agttgcggga taactaagcg aatgacattt ccatcattgg ttggactgat
      301 tccgatatca cttgccataa tagctttttc aacatcttga agaatgctct tgtcaaaagg
      361 tgtgatcatt aaaacacgtg cttctggaat ttggattgat gccatttggt tcaatggtgt
      421 tggcgcaccg tagtagttca cagtaatacg gtccaaaagg ctagcgttcg cacggcctgc
      481 gcggatttgc ccaagttcac gttgaagatt ttgtgctgct tttttcattt tttcttttgc
      541 ttctgtcata attgcatctg ccatttttat ttccccctta cagttgttcc gatattttcg
      601 cctaagattg cacggcgaat atttccgtgt tcatttaaat tgaagacaag cagtggaata
      661 tcattatcca tgcttagtga acttgcagtt gaatccatga cttgtaatcc tttggcaata
      721 acttccagat gagtcagttc ttcaaatttt actgcatttg catctacttt cggatcagca
      781 gaataaacac cgtctacatt gtttttagcc atcaggataa catctgcgcc gatttctgcc
      841 gcgcgtaatg ctgctgttgt gtctgtcgag aagtacgggt tgccggtacc gcctgcaaag
      901 ataacgatcc gtcctttttc taaatgacgt tctgctttac ggcgaatata tggctctgcg
      961 atttgacgca tttcaatcga agtttgcacg cgtgtcggta cacccacgtt ttccaacgta
     1021 tcctgtaaag ctaaggcatt cataactgta gctaacatac ccatgtaatc agcttgagca
     1081 cgttccatgc ccatttgtgc tccgatttgt ccgcgccaga tatttccgcc gcccacaacg
     1141 attgccattt caacgcccag ctcgtgaact tctttgattt cttggatgat ttctttgatt
     1201 actggtggtt tgattccaaa tcctgcgtcc ccagctaaag cttctccact caattttaag
     1261 actacacgct gatatttcgg tgtgaccatg ttcattcctc caatattttc tattgccatt
     1321 ttaccatact tagatgattt cgacactcta agactggttt ttcttgtttt gcttaaaaaa
     1381 agggagcgca caaaccgtgc gttcccgtct gttattcaac agaattattt tttcacttgg
     1441 ctcataactt cttctacaaa gttatcttcg cgtttttcga taccttcgcc aacttcgaaa
     1501 cgtacgaatg ttttaacagt tgcgcctttt gatgcaacgt atttttcaac agtcatatca
     1561 ggatctttaa cgaatggttg gtccaccaaa gcgatttctg ctttgaattt cttcaagcgt
     1621 ccttctacca ttttttcaac gatattcgct ggtttgcctt cgttcaatgc ttgttctgta
     1681 agaacagttt tttcatgttc taattcagct tctggaattt gtgattcgtt tacataacga
     1741 ggattgatag ctgctacgtg catagctacg tctcttgcaa ctgtttcgtc agttgttcca
     1801 tctagaacag tcaatacggc aatacgtccg cccatatgca agtatccacc aaatgcagca
     1861 ttgtcgtctt tttctacaac ttcaaagcga cggaagctga ttttttctcc gataacttga
     1921 gttgcttcga tcaagtcaga ttcaattgta cctttatctg ttttgatttt cattgcagct
     1981 tccatgtctg ctggtttgtt ttcagcaaca agttctgcaa tttcttttac taaatcttgg
     2041 aacatttcgt ttttagaaac aaagtctgtt tctgagttta cttcaacgat tgctgcagtg
     2101 ttacctttca cagcaactga agccaatcct tcagctgcaa tgcggtcatt tttcttcgca
     2161 gcctttgcca ttcctttttc acgtaaaaga tcaactgctt tttccatgtc gccttctact
     2221 tcaactaatg cttttttcgc atccatcatt cctacgccag tcatttcgcg tagttctttt
     2281 accattttag ctgtaacgtc tgccatgttt aactcctcct gtgtgttttt ctttttaaaa
     2341 aaagctgctt caaaggagag tgaggcttgt cgcccagcct ttgaaacagc tccatcattc
     2401 aaaattattc tgctgaagaa ttgtcgcctt caacaacatc tacgatttct tcgattgatg
     2461 ttgctgtgtt ttcttctgtg aaatcttctt caactacttg atcttcacct tggtttcctt
     2521 cgatgaaagc atcagccatt tttgaagtaa tcaatttaac ggcacgaatt gcatcgtcgt
     2581 ttgaagggat tactacgtcg atttcatctg gatcgcagtt tgtatcaacc atagctacga
     2641 ttgggatatt taatttgtgt gcttcttgaa cagcaatacg ttctttacgt gggtcaacga
     2701 tgtacattac atctgggatt cttggcatat cagcgatacc gcctaagaat ttttcaagac
     2761 gttcacgttc tttgttcaaa ccagcaactt cttttttagg tagaacttca aaagttccat
     2821 cttcttccat tgcattgatt tgtttcaaac ggctgatacg tttttggata gtatcccagt
     2881 ttgtcaatgt tccgcctaac caacgatggt taacgaagta ttgtcctgca cgagttgctt
     2941 cttctttgat tgcttcttgt gcttgttttt ttgtacctac gaataatgct acgccgcctt
     3001 cttcagcaac gctcttcatg taatcataag ctgcatctac taatttaact gttttttgca
     3061 agtcaatgat gtagattccg tttctttctg tgaagatata tttcttcatt tttggattcc
     3121 agcgacgagt ttggtgacca aagtgtacgc cggcttctag taattgtttc atagaaatta
     3181 ctgccatgtt tgtttcctcc gatttggttt tatttttcct cttcttatct tctaccttgc
     3241 cggcaaactt tttcaagcac ctaccgacaa tccaataaga atgtggattt gctgatgcat
     3301 aaagcaccct cagctattat acagacaaaa gatccgtatc gcaagagcaa atatactttt
     3361 ctgctcaaca ttcaatagtt tacctgaaag ataaaaagaa ttcaagaaaa acagtagatt
     3421 tctctttgtt tctttattat cctttttcca taaaataatt ttaggtataa aagacgaata
     3481 ttattgggca atccttcttt tttttgttat gatacatgtg aaatctttcg taaaggagaa
     3541 agaaaatgcg caaagctcaa agacatttgc tgattaaaca agtcattgaa gaattcccga
     3601 tccgtacgca agaagaacta ttaaacaaac ttgggcatta tggcgttacc gccacgcagg
     3661 caacgatttc tcgagatatc cgtgatctaa aaatcgtgaa ggcacctgat gaaaatggcg
     3721 tttcccgctt cgtattgttt cgcgaaaaag gaactcctga gactaggact gaggaagagg
     3781 atcggctggt tcaaatgatt gaagacattg tattgaaagt cgaacgagtc cactttttga
     3841 cgatcgtcaa taccttgcca gacaatgctc atttatttgc atctgtttta gatgagatca
     3901 aacctccctc tattgtcagt acaattgctg gattcgatac gatcatggtc atctcggaaa
     3961 atgaggagga cgcaaaacga gtagaagaat ttcttcacca tccaactgaa gtatcacttt
     4021 tttaatcaga gcacgtattc ctaatatgga atgcgtgttt ttttattttc aatattttcc
     4081 ttattgaata aacctttaca ctctttacaa aaatacatca ttttttattc aaatttttca
     4141 acaccttgat tgaatcacaa taatcatcat ttatattgga tgttaacaag agcagcttct
     4201 tgctctttgt gtatttcaaa aaagaaatga aaggaaatca tatgtataaa ttattaactt
     4261 ctggaccatt gacgacttct gaaacagtga aaaatgctat gctgatcgat cattgtactt
     4321 gggatcaaga atacaaagaa attacacaat ggataagaaa aaaattattg gatcttgctc
     4381 aagtatcgga aggaatatac acaacggtct taatgcaagg tagcagctcc ttcggggtag
     4441 aatcggtatt aagttctgtg atcaggccag aagatactgt actgatttgt tcaaacggcg
     4501 catatagaca acgaatgatc aacatgtgcg aatgtctgaa actgaactat accgtttatg
     4561 aggtatcgga acatcaaatc ctccaagctc atgtgattac acagctgatt gaagagaatc
     4621 cgtctatctc cgcactagca atggtccata gcgagactac ttccggtatt ttgaataatt
     4681 tggaagagat tgctgctgtc acacaagaaa gagatctttt gtttatagtg gacgcgatgt
     4741 caagctttgg tgggataccg atttgtgtag ccgaactagg gattgatttt ttgatttcca
     4801 gctcaaacaa atgtattcaa ggtgttccag gcttttcttt tgtacttgct aataaagcgg
     4861 aactggaaaa acgaaaaaat gttagccgaa gtttgtcact tgatctatat gaccagtacc
     4921 gcgtgatgga aaacgagaat ggcaaatggc gtttcacttc ccctacccat acggttttgg
     4981 cttttcaaca agcgctgaag gaactagagg cagaaggagg aatcgaaact cgatattcac
     5041 gttatctcgc caataatcat gtgctacgac aaaaaatgac agagatgggg ttccagcctt
     5101 tcattcaaga atatcagggg ccattcatca cctcttttct ttaccctaag gactggcgat
     5161 ttgattttaa tcatttttat cagtatttaa agcaaaatgg atttgtgatc tatcccggga
     5221 aaatcagcga tcatgaatgt tttcgtatcg gaaatattgg cgagatctat ccggaagata
     5281 ttttggaact gacagcaatt atcaaagact ataaggagaa cgttaatgat taaaactgta
     5341 atttttgatt gggcaggaac aactgtggac tttggctgta tggctcctgt ccacgctttt
     5401 agaaatgctt ttctagaaaa aggaatccaa ctgactgaca aagaaatccg ctaaccgatg
     5461 ggaaaattaa aatgggatca tatccaacag cttctttccc tcccttctgt caaaaaacaa
     5521 tggatagcta tttatggaca actacctcag gaaaaagagg tggaggaatt gtatcaacaa
     5581 tttgaacatt atctgtttaa agatttggtc aagcattcta ccttgaaacc cgacacattg
     5641 gaaacgatcg aacggttgag aaacgctggg atcaatattg gttccacgaa tggatttaat
     5701 gctcctatga tgacaatcgt agcagaaacc gcaaaaaaag aaaggtattc accagacttc
     5761 atggcaactt ttgaagacgt cgaagggtat ggtcgaccgt ttccttatat gatccataaa
     5821 aacatgcagt acttcaaaaa caagtcggtt gatgaagtaa tcaaagtcgg agatacggtc
     5881 tcagatatcc aggaagggaa aaatgccgga gtgttgacag taggtgtaat tgaaggtagt
     5941 tcgctaatgg ggatttctta tgatgagtac caaaaattag agatggggaa aaagatcgtt
     6001 taaaacagtc tgttgctctt agattccaac aagccggcgc tgatatttgt gtaaacaatt
     6061 taaaagagtt aacagaatac attttgtacg ctgacatgaa tccagaatga cttttataca
     6121 aaataagagg ttgtgaaaaa gctgtcctgt atcaagtaat aaaaacagcc aagcaaaaat
     6181 agctcctcat atttttcgtt tggttaggct tattaccgca gatgcaagct tttgaacacc
     6241 gtttattcga aaaaaaaaag ggagctgtga cacgactttt gtcacagtcc ccttatttgc
     6301 aaacaaacag tgtttaagag acaacctctc cactaaacgg tgttcattca gctgactctc
     6361 ggtatttgct caaaaaacga taaaatttga aaagctattt tctcttttgt ttctctaata
     6421 ctcggatcag aacgctgatt tcacattctc tttttcttac atcttatctg gtgcatgaag
     6481 tcctagcaaa cgaaggtctt ctttcaataa tacagtcact gcataaacta gagctaagcg
     6541 tgcttctttt tgttcatcat cagctaaaat cttcgtatga gcatagtatt tgttgaatgc
     6601 ttgtgctaat ttgattgcat gtttcgcaat cactgacggt tcgtattttt ccccagcaga
     6661 caagaccgtt tcagggtatt tttgaactaa tttaaccact tcccagctgg tatcgtcatt
     6721 caatgcatag tctgctgttt ctgaagggac aaatcctgct ttttccaaca ggctgactgc
     6781 acgagcatgt gtatattgca cataaggtcc agtttctcct tcaaatcgaa caacttcttc
     6841 caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa tcgtggaaaa tgactgcccc
     6901 aaccccaact tgtttagcta cagcatcttt gttttccaaa tcagggtttt tctcgctgat
     6961 ttgttcttta gcagattgga ttgcttcatt taatacttct tctaataaga cgatctttcc
     7021 tttacgagtt gataattttt taccaccttg cgtgatcaaa ccaaatggaa tatggtgcat
     7081 gtcgtcagac cagtcaaagc ccatttcctt caagacagct ttcaactgtt taaaatgata
     7141 gctttgttca ttccctacta catatagaga ttgtttgaag tcgtaagtac gtttacggta
     7201 caatgcagca gctaagtcac gtgtaatgta tagtgtcgca ccgtctgact tcttaatcaa
     7261 cgctggattc aggtcataag cagataggtc aacgatctct gcacctttat cctcgttcag
     7321 caaatgtttt tcttctaaaa gtttgacgat ttcatccatc ttgtcattgt agaaagcttc
     7381 accgttcaac gaatcgaaac gtacttccag cagatcatag attttattga attctttcat
     7441 cgattcatca cggaaccatt gccataattg aatagcctct ttatcgcctt cttccagacg
     7501 tttgaaccaa gcacgagctt cttcatttaa ttctggttct gtttcagcta cttcatggaa
     7561 ttggacgtat aaacgaagca attcattgat tggttctgct ttaacagctt cttctgtacc
     7621 ccattttttg taagcgacga tcagttttcc aaactgtgtt ccccagtcac ctaaatggtt
     7681 gatgcggatt ggctgataac cgattttttc cataatgaaa ccgatcgaat taccgataac
     7741 tgtcgaacgc aaatgtccca ttgagattgg tttggcaata ttaggagaag acatgtcgat
     7801 tggtactgtt ccttgattcc cgatgttgct atccccataa tgttcttttt ctttcactac
     7861 tgtttgcaaa acttttttgc tgataagttc tttattcatg aaaaagttca agtaaggtcc
     7921 gacaacttcg attttttcaa agttggcaga atcgattttt tctgctaaat ctgcagcgat
     7981 ttgctgtggc gcttttcggt aaacttttgc taatgaaaag gctggaaatg caacatctcc
     8041 gtgttcagct gattttggat tttctagtaa ttgctcgact tgttccagtg ttaaatcatc
     8101 tttgacagcg tcatagacag cttttgcgac aagatcttta ttattcatct ttattcctcc
     8161 taaagtgttc ccttgattgt taaacaaaaa aaatctctag cgacggcata cttagtatgc
     8221 catcactaga gacgagattt cccgcggtac cactccaatt gtcctaaaaa aggaccagct
     8281 ccatctgata tcggtagacg accgtctgtt taaaacagaa ttctccaagt gcgcttcgtt
     8341 gtatcttctt cattcggctt ccaccacttc cgaattcgct agccaagaat gatacaagta
     8401 ctctcttgtt caacaaatct tagtcttatt aaactttctt gaattatatc agaaaaaaaa
     8461 acgaaaggct agtcgtttca taaaaaaaca atccagatgg cctaaaggag aagaaatacc
     8521 acctggatag ctataaatca tttcgtaaat acgaaagact taacaaatta atcagctacg
     8581 ataatcgttc cttcttcaga agctaaaaga ttctcgatat tttctaaaga agtgatgatt
     8641 gccttagcgt ttggttttgc ttcaacaaat gcaatagctg cttctacttt cggaagcatg
     8701 cttcctggag cgaattgctt ttcatcaatg tattgtttca tttctgaaac agtaacagtt
     8761 tctaatttct tttgatctgg tttttggtaa ttgacataca cattgtctac tcctgtcaat
     8821 acgatcagca aatccgcatc gatgatttcg gctaattttt ctgaagcaaa atctttatcg
     8881 attacggctt ctcgtccttc taatccagta gctgtttcta caacaggaat cccaccgcca
     8941 ccaacagaga ctgtgataac tccttgatcg accagcgttt cgatgacacg tgcttcttta
     9001 atcgagatgg gtttagggga agcgactact tttctccatc cacgtccagc atcttctttg
     9061 aatgtcacag tggagtcagc attcatttgt tctttggctt cttcttccgt gtagaatgga
     9121 ccgactggtt tacttggatt tttgaatgaa ggatcatttt catccacaat aacttgagtg
     9181 actaaagaaa ccacatcttt atcgatccct ttttccttca gtacttcgcc catggcgttt
     9241 tgtaaccagt aaccgataga tccttctgtc attgcaacac atgtatctaa aggcattgcc
     9301 ggtgttttct cagaatcagc agcttgctgc tgaatcaata ggttaccgac ttgcggacca
     9361 tttccgtggg agatgatcaa ttcatctcct tgttcgataa atttcactaa atactttgct
     9421 gtttccatta acgcttcttg ttgggctttt gcacttgcat ccgtagacaa gatggcattg
     9481 ccccctaaag cgacaactac ttttcgtttg gccatttcat tcgtcctttc ttcagttcta
     9541 ccgaattttc tagataacaa aacgagccag cgttttgtga ttttccctca atgctgactc
     9601 gttttttcgt gagatgaatg tggttatgct taaacgcgag ggataaacaa gttacctaat
     9661 gttgctgcca tgatcgcttt gattgagtgc atacgatttt ctgcttggtc aaattgacga
     9721 gcatatttgc tgcggaagac ttcatctgtt acttccattt cagtgatgcc aaagcgttct
     9781 ttcatttgtt caccgtaaac agtgtttgta tcatggaaag ctggcaagca gtgcaagaag
     9841 atcaaacgat ctgtgttgtg tgttttttct accatttcca tattgatttg gtaaggtttc
     9901 aacaatttga tacgttcttc gaatttatct tcttcaccca ttgataccca tacatcagaa
     9961 taaaggacgt ctgctccgtc tacaccttta tcaacatcgt cggtaatcat caattgtgca
    10021 ccggattttt cagcaaagcc ttctgccatt ttgacgattt cttcttcagg ttgtaattct
    10081 tttggtgcaa cgatacgcat attcgctcct aaaattgcac ctgtgactaa taaagagtta
    10141 gccatattgt ttcgaccatc tccgcagtaa gcaacagtga taccttctac tgttccaaag
    10201 ttttcttgga tagttaaaaa gtcagcgatc atttgtgttg gatgccattc atcagtcaag
    10261 ccattccaaa ctggaacacc agagaattct gctagttcct ctaccatttt ttgactgaat
    10321 ccacggaatt cgattccgtc aaacatacgt cctagtactt tagctgtatc ttctgtggat
    10381 tcttttttac ctaattggat atcatttgca cctagatatt caggatgtgc acctagatcg
    10441 atcgctgcag tagtaaatgc agaacgagta cgtgtagatg ttttttcaaa taaaagcgca
    10501 atattttttc cttctagata gtgatgtggg ataccacgtt ttttcaagtc tttcaagtgt
    10561 tctgaaaaat cgatcaaata ttgtaattct tcttttgtaa agtctttttc tgctaataag
    10621 cttcttcctt ggaatactga ttctttcata ttaaacattc tcctttagat tgtttttatt
    10681 taagattaaa gaggaaatcc cgacggcagc tgacagtaag actgccgtca ggaaacaaat
    10741 tacttcaagt cttcacggac aagtggttgg ctcatgcagc gtggaccacc acgacctcgt
    10801 gacaattcac ttgaattgat ttctaacact ttgatgccat agctacgaag caattcgttc
    10861 gatacataat ttcggttata agtgactaca acacctggag cgattgccaa tgtgtttgat
    10921 ccatcattcc attgttctcg aggtgcaacg atctcatctc cattaccagt tgggatcagt
    10981 acaagatcat ctaatccaag agcagctttc atcgtttcgt gtaagttatc tgaatgagta
    11041 atcttgatat catcaccatc tggttcgatt gtaaagacat ccatcttacc gtctttactt
    11101 tgaattgctg gatggatagt gaatttatcg tgatctacca ttgtaaatac ggtatccaaa
    11161 tgcatcattg cacggttgtt cggaattttg attgccaaca ctttttcaaa tcctgaattt
    11221 tttgcgaata ggtggcgtgc taatttttct aaagctttcg cattagttcg ttgagaaatc
    11281 ccaacagcta ctactttatc gcttaagatc aattcgtctc cgccttcgat atgatctgga
    11341 ttttcacgat ccaaccatac ttcgattcct ttatttgcaa atcttggatg atgtttcaaa
    11401 atatattctg taaacatcga ttcacgacga cgtgcttcaa aagtcatctt attgactgtc
    11461 atcccatttc ccattgatgc ggaagggtcg cgtgtaaagt acaagtttgg cattgggtcc
    11521 atgtaaaatg gatagtcatc gtctgctgac agatcatata gactgctaga acgtacttcg
    11581 atatcttttg tacgaacccc agccatgatt ttatctacca tttcttgcgt ttccattgat
    11641 agaaggaact catgaagacc ctctcttaca gcattcgatg caatatgaga ttcatccaac
    11701 attttattca agaattgttc tttgacattg cctgcgtcaa tcgcttctgc cgctaatttt
    11761 tccaagtaaa gagtctcgac gccttcattt tgcaatgtct tcgcaaagtt gtcatgttct
    11821 tcttgagcaa tcggaagata tggaatatca tcgaacaaca gacgatccat aatatctggt
    11881 gtcaaatttt ccacttcttg tccaggccgt ttcagtaaaa ctgtctttaa tttccctatt
    11941 tcagagaaaa cgtgaatagg tttatccatg ttgtgttgcc tccttctttt tgattacatg
    12001 tataggatat cctccttttg taatcacttt cataattttg tctcattcaa aatgagacat
    12061 tcctcacatt aatattcgat aattaaataa ttaaacgttt ttctattttt aagcgtaatt
    12121 tattcattt
//
LOCUS       DAZUKZ010000075        12018 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000075 DAZUKZ010000000
VERSION     DAZUKZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.077"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..724)
                     /locus_tag="ACG8EF_001978"
     CDS             complement(137..724)
                     /locus_tag="ACG8EF_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947275.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            899..2221
                     /locus_tag="ACG8EF_001979"
     CDS             899..2221
                     /locus_tag="ACG8EF_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4947276.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2376..3152
                     /locus_tag="ACG8EF_001980"
     CDS             2376..3152
                     /locus_tag="ACG8EF_001980"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS4947277.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3145..3714
                     /gene="rnmV"
                     /locus_tag="ACG8EF_001981"
     CDS             3145..3714
                     /gene="rnmV"
                     /locus_tag="ACG8EF_001981"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFS4947278.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3707..4591
                     /gene="rsmA"
                     /locus_tag="ACG8EF_001982"
     CDS             3707..4591
                     /gene="rsmA"
                     /locus_tag="ACG8EF_001982"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFS4947279.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5042..5671
                     /locus_tag="ACG8EF_001983"
     CDS             5042..5671
                     /locus_tag="ACG8EF_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947280.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(5795..6904)
                     /locus_tag="ACG8EF_001984"
     CDS             complement(5795..6904)
                     /locus_tag="ACG8EF_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4947281.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7113..7535
                     /gene="mgsA"
                     /locus_tag="ACG8EF_001985"
     CDS             7113..7535
                     /gene="mgsA"
                     /locus_tag="ACG8EF_001985"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFS4947282.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      7722..7820
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            7858..8394
                     /locus_tag="ACG8EF_001986"
     CDS             7858..8394
                     /locus_tag="ACG8EF_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS4947283.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8434..10197)
                     /locus_tag="ACG8EF_001987"
     CDS             complement(8434..10197)
                     /locus_tag="ACG8EF_001987"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4947284.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10200..11915)
                     /locus_tag="ACG8EF_001988"
     CDS             complement(10200..11915)
                     /locus_tag="ACG8EF_001988"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4947285.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         3742 a         2222 c         2454 g         3600 t
ORIGIN      
        1 gatgtgttaa ttataagttc tatcacttta tgttcaatta gtttgtgctc ttatatgtgt
       61 gattgaaaat atagataaaa aaacaatcat cggctttgac ttcgatgatt gttttcttta
      121 ttttgtaaaa atcctatcac ttgattttat ggtaaccttt tatttttgtc ttcaaacggc
      181 ttggtaagag agcagtaagc agtgcaaatg ggatcgaaaa gacataccag aaaatcgtac
      241 gtccgatacc ataatgttga tagagctttg tcaacatcac gtgtaacaag atcaacccta
      301 aaagcaagga gccagctata aaccaatgat tatcccaatg catgacttca ctaagtgcca
      361 tcactccaaa aggatagaaa taaaaataga tcccgactaa ggtcatccgc cagttctttg
      421 taaatgttcc gatattcaat aaaaagaaaa tcgctgaaag caaaataccg ataggcggaa
      481 aaagcccaac caaaaaggaa tacaacatcc cccacaagat catcacagga cctttgatca
      541 atgcaacgat tgcgatgaac aaagtcagca atattagttt ttgtgataac gttgcaacag
      601 tcattaatcc aaataaaatt aatgtgaacc aatgccaatg ggactgctgt tgttctttac
      661 gggaaaaatc gacccggttg gataaataat ctgctaattt cggataagaa cgcttaaaat
      721 ccataaactg ttcctccttc gtggaaaagg taggtttagt atacgaaaaa taaaaaagaa
      781 atacatccgt cttaagaggg agtttttcac tgaaagtgga gaaaaaagaa tggtaaatta
      841 aaatattctc agttatactg aaaattatac caaatttcaa agaagaaaga ggatgggaat
      901 gaaaggcaaa ctaaaacgag aaatcaatct gtttggcgca ttagctaccg ttatgggaac
      961 cgtgatcggt gcaggcgtct tcttcaaaac tgctgctgtt actgctagta ctcagtctac
     1021 aagtttgaca ttgctagctt ggcttttggg tggtttctta acgatctgtg ctggattgac
     1081 agtggctgaa ttagctacag caatcccgga aactggtgga gctgtaaaat atattgaagc
     1141 tgcgtatgga aaattgccca gctttttatt aggatgggcg caaagcttga tttactttcc
     1201 tgctaatatt gctgcattat cgattatctt tgcgacacaa ttgacgaact tattacaatt
     1261 atctaccgat tatcttttgt tgattgcagt gatcaccgct gtttctgtga cagggttgaa
     1321 tttgttaggg acaaaagtcg gtgcttctgt acaatcagtg acgttgattg ttaaattaat
     1381 accaattgct gtgattgtca tatggggatt attgacccca ggacaaggaa ctgtccagtt
     1441 atttccaatc gaagctggaa aagatgttac ttttgtcgaa ggattgagca gtgcgttgtt
     1501 agcaacgttg tttgcttatg acggctggtt aggtgtcggt gcaatggcag gggaaatgaa
     1561 acggccggaa aaagatttgc ctaaagcaat tattcttggt ttgagttttg taacagtcgt
     1621 ttatctattg atcaattttg tttttttgaa aactttgccg attgatcatc ttgccggtaa
     1681 tttgaatgca gcttctgaag catcagacgt catttttgga ggaatcggtg gaaagctggt
     1741 cactattggg atcttgatct cggtatacgg tgcattgaac ggttatacac taactgggat
     1801 ccgcgtgcct tatgcgatgg ctttagaaga cgagctacct ttcagtaaac agctgacaaa
     1861 gctttcaaag aagtttacgg ttccttatgt tcctgcggtt ttccagttag cagtagcttg
     1921 tattatgatg agccttggtt ctttcgactt tttaacagac atgttgattt ttgtgatgtg
     1981 gctattcagt ttactgattt ttatcggtgt ctttatttta agaaaaaaag cgccggaatt
     2041 gccacgtcca tataaagtac cactttatcc aatcgttccg attattgcga ttctcggtgc
     2101 tattttcatt ttaggtatga cgatgatgac gcaaacgaaa ttagcattga tcggtatagg
     2161 tgttaccttg ataggtatcc cagtctatta tcaaaaaaag aaaaaacgct cagaagagta
     2221 aaatatttca acaacagaca gaaaaagtac gaactaggtc ctctagatct tcgtgctttt
     2281 tctatttcaa taaaacaagt tcttctattt tgatttttgc tacttctgca agtagtgctt
     2341 atgtgctaaa gtaagaatga aaaggagaga taaacatgat atttgattct catacccatt
     2401 taaatgctga acagttcaat gaagatattc cagaaacgat tcagcgagca caagaaatgg
     2461 gggtaacgaa aatggcggtc gtgggcttcg atacgccaac gattgaaaag tctttgcaat
     2521 taagccatga ctatccaaat atatacagta ttatcggctg gcatccgaca gaggctggca
     2581 gttatacgaa agaaatcgaa aataaattac aagaacaatt gactttacca aaggttgtag
     2641 cattagggga aattggcttg gattatcatt ggatggaaga cccgaaagaa gtccaagata
     2701 aagtatttcg cagacagatt gctatcgcaa aagaaatgaa cctaccaatc agtattcata
     2761 ctagagaagc acttgaagac acgtatcaaa tcctaaaaga agaagatgtt cgagatatcg
     2821 gtggaatcat gcatagtttc agtggtgatt atgaatgggc aaaacgattt cttgacttag
     2881 ggatgcatat ttcatttagc ggtgtcgtca ctttcaaaaa agcattagac gtccaagagg
     2941 ctgcagcaaa tgtaccaatc gaacgattac ttgtggaaac agatgcccct tatctggcac
     3001 cagttccttt tagaggaaag cgaaatgaac caggctatac tcgttataca gtagaaaaga
     3061 ttgctgaact tcggaatctt ccgacagaag aagtggctgc ccaaacatgg acaaatgcgc
     3121 accgattatt tggtctaagc gaacatggat aagatgaaga tccaagagat tatagtcgtt
     3181 gaaggaaaag acgatacaag aaggctgcgg gaagtagttg atgcagatac gatagaaaca
     3241 atagggtctg caatcaatga agagatcctg atgcaaattg aacatgccca agagactcgt
     3301 ggagtgatcg tttttaccga tcctgatttt tcaggagaaa aaataagaaa aatcattatg
     3361 gaagctgttc ctcaggcaaa acatgctttt ttgccgcgca cacaagctaa accaaagaaa
     3421 aagggaagcc ttggtgtcga gcatgcaagt gatgctgcta ttttagaagc attgaaaaag
     3481 gtcgtgacgc cagacatttc ccaaacggct gtttctgaaa tcactagaca agacctttta
     3541 gactatggtt taatagctgg ggcacaagca aaagaaaaac gagaaaaact aggtgacgag
     3601 ttgcgcatcg gttacacgaa cggcaaacaa ttggaaaaac gtttgaagat gtttcgtatt
     3661 acgccgaaag aactcgccca agcaatgaaa atagtggagg aacaacatga gtgaatatcg
     3721 tgaaattgcg acaccctcta gaacaaaaga gattttattg aaacatggtt tttcatttaa
     3781 aaaaagttta ggacaaaatt ttctgactga accaaatatt ttaaggaaaa tcgtagaaac
     3841 tgcgcaaata gatgaacata cgaacgtgat cgaagttgga ccaggaatcg gtgcattgac
     3901 ggaacaatta gccaagcatg caaaacaagt cgtagcattt gagatcgatg atcgtttgat
     3961 ccctgtattg gctgatacga tgcaacccta tgacaatgtg actattgtac atcaagatat
     4021 tttaaagacc gatttgtcaa cagctgttag agaaacattt catgaggagc ttccgttaaa
     4081 agtcgtagcg aatttacctt actatattac gacaccgatc atgatgcatt ttcttgaatc
     4141 agatttggtt gtggatgaac tagtcgtcat gatgcaaaaa gaagtagctg atcgtatctc
     4201 tgctgaacca ggaacgaaag cttatggttc attatcgatt gctgttcaat attatatgga
     4261 agccagttta gcattcatcg taccaaaaac agtatttgtc cctcagccaa atgtagattc
     4321 agcgattttg aaactgacaa gaagagatat accggcagtc gaagtgacag acgaaaaagc
     4381 atttttccga ttgacaaaag cggcatttca gcaaagaaga aaaacgcttt ggaataactt
     4441 gcagcattcg tatggcaaag atgaccagac aaaagcatgg ttagcaaaaa gcttggaaac
     4501 agctggaatc gatccaaaac gtcgtgggga gactttgtca ttacaagaat tcgccgcatt
     4561 aagcaatgcg atgtcagaaa atatgcagta aaaaaagagg ttgtgacaga agttgtgaaa
     4621 aagctgtcct gcatcaagta ataagaacag ccaaacaaaa atagttcctc atgtttttcg
     4681 tttgtttggg cttgttaccg cagatgcaag cttttgaaca ccgtttaaga aagaggttgt
     4741 gggcctgccg tttaactcta agtcacaagg aacaatttca aaaaacagct tctcatgttt
     4801 ttgagttagt tggtcttatt acttgatgca agcttttgac cactattatg ccgaacaagg
     4861 agatgtgaca caaattttgt cacatctcct atttttgttt cttgatcaaa aaattcttat
     4921 acttgatact cacactcatt ttgtatggat ttagaaaaga atatgtgtct tgacagttat
     4981 tagtaagtga gttatactaa gtcgacattt atgtgtcaag acatattagg agagagaaaa
     5041 aatgagaaaa aattcagttc gaaaactaac gatttctgct ttattgattg ggatgggggt
     5101 cattatccca atggtcatgc ctaaaatcat gattccacca gcttccttta ctttggcgtc
     5161 tcatgtgcca ttatttattg cgatgttttt tacaccagga gttgcagtgg ctgttgcgct
     5221 aggtacgaca tttggtttct ttttaacaac gccggttatc atcgcattgc gagcattgtc
     5281 acatcttgtg tttgcgttga ttggggcttg gtacttgcaa aagcatccga gtatcgtttt
     5341 aaaaaatgga cagtttactt ttgctaattg gcgtttccaa gggttcaatt ttgtgatcgg
     5401 tgtgattcat tctttagcag aaatgctagt agtcagcatc ttttacttta ttggtaatat
     5461 gcctgaaaca tattattctc aaggatattt ttatacagta ttcatcttga tggggattgg
     5521 tgggcttatc cacagtttag ttgattataa tattgcttat tttatagcag gaacactcag
     5581 caaatatttt gatatccccg tatttacggc agcaaaaaaa gaaaatcgga aagagtcgat
     5641 tcaagggtac gcgaagaaaa cgacatatta gacatgatct ctcaaaaaaa aaactctcat
     5701 ctagtaatgg ataataaatc ctctaagatg agagtttttt gtcctaaaaa gagctgcacc
     5761 aacatcttct ggcacagctc taacaggggg caatttatgg aagtttgatg acttgttctg
     5821 tttctttatc aaagaagtgg cctttgctga tgttgaaggc aaggtcgact atttcgcctg
     5881 gtttatggaa atctcttgca tctacttttg agatgaactc agtagaacca gtttttgtgt
     5941 aaagcatcgt ttcagctcca agtaattcgg aaacgacaac ttctgatttc actacggctt
     6001 caggtgaagc ttcaagggct acttgttcac tgtggatgtc ttctggacga ataccgaaga
     6061 taacttcttt tccttcataa cctttttcaa ctaatacttt gttttttcct tcaggaatac
     6121 gaagcttaag cccatgtcca tctgtgataa caccattttt caatgtcaca ttaaagaagt
     6181 tcatggcagg agatccaata aagccggcaa cgaacatatt gacgggtgta tcataaactt
     6241 ctttcggtgt accgatttgt tggataaatc cgtctttcat gataacgatt cggtcagcca
     6301 tagtcattgc ttctgtctga tcgtgagtaa catagatggt cgttgtttca agacgctggt
     6361 gaagtttggc aatctcagca cgcatggcta cacgtaattt agcatctaaa tttgataatg
     6421 gctcatccat taagaagact tttgcatccc gaacgattgc ccgtcccaat gccacacgtt
     6481 ggcgttgtcc accagataaa gcagctggtt ttcgtttcaa gtattctgtc agacctaaga
     6541 tctctgctgc attgtccaca cgttttttga tgtctgcttt atcatattta cgtaatttca
     6601 agccaaaagc catattatca aaaacagtca tatgaggata caatgcgtag ttttggaaaa
     6661 ccatggcaat atcacggtct ttaggtgcca cgtcattcat gacttttccg ccgatattta
     6721 gttctccttc agagatatct tccagtcccg cgatcattcg caaagtagtt gactttccac
     6781 aaccggaagg accaacaaaa acgataaatt cacggtcttt gattgataga ttaaaatcag
     6841 ttacagaata attttctgca ttgtcatatt ttttatatac gtttttcaga gccatttcta
     6901 ccataatacg ttccacactt tcttttttag ttttattaac tatttaaagt ataaatgaaa
     6961 gcgttaacta acttcaatgt catgttgaac aagaattttt tctttttttt gtcaatgtgc
     7021 tagttttaag ctttaataaa tatccataag aaataaaaac tgatacactg gagtaaataa
     7081 gtatgttaac aatatcaaaa ggagtgtctc ttatgaaaat cgctttgatc gctcatgacc
     7141 gaaaaaaacc tatgatggtg aaattagcaa cagcgtataa aacaatattg gaaaaacatg
     7201 aactttttgc taccgggacg actgggcaga ggatcacgga agcaaccggc ttgccgatcc
     7261 atcggtttaa atccggaccg ttaggcgggg atcagcaaat tggagccatg atttcggagg
     7321 atcagttaga tttggtgatt tttcttcgag acccgctggc tgcacagccc catgaaccag
     7381 atgtaacagc acttatccgt ttatctgatg tctatgagat tccgttagct acgaatatcg
     7441 gaacagctga ggtgctacta agaggactgg aagccggctt cctagatttt cgaagaatcg
     7501 tccatgaaat agacaagcat ccactttcat tttgatggat aagcgtatgg aaaaataacg
     7561 tttaatgttc gcctatatgt agctataaaa gctaaacaaa ctgataatat gcgatataat
     7621 acgaacgtat tggataatat aaacaataat attagttttt cgtatttgca gtaaatgtga
     7681 aaatatgcta tagtttctgt gggaaaagat cccaaaaata atacagagta gaaaataaag
     7741 cgttaagtgc taagaggatg ggatgttgcc tcttggacga aggacgtaat gttcgcggtt
     7801 ttattttcgc attcgctgca tgatacgatg tagcagctct taaaggagat gctaaaaatg
     7861 aagaaacacc ggttagacgc acgtatgatt gctattatgg gcttattgat tgcattgatg
     7921 gtcactttgt cccgactggt agcaatcgag accccattta ttaaaatcag tgtcacattt
     7981 attccacaag tgatcatggg cattcttttt ggtccttttt ggtcagggat aggcgcagtt
     8041 ttagctgatt tagtaggaat ggccctcttc tcgaaatctg cattctttat tggatttaca
     8101 ttgaatgcat ttattgaagg agcaatctac ggattctttt tctatcgaaa agagatcact
     8161 tggaaaaatg ccattctggc tacgcttagt gtcacgctga tcatcaactt atttttgaca
     8221 ccattatggc ttgcgttgat gtaccatgtg ccattgttca gctgggtcgt ttgggcgccc
     8281 cgcctgttaa aaacagtaat atggttaccg attcaatcaa ttgccattta ttacgtagga
     8341 cgtagtattc cttacaaaaa aattttaaga agtttagcga tccacgctaa ataaaaaaca
     8401 agcagtcttc ccatcccaga agactgcttg tttttattgt gctgtgaatt gtgcttgata
     8461 gagccgtcgg taataaccat ctttttgaag cagctcatca tgggtgccca tttcgacaat
     8521 agctccctga tccatcacta atatctggtc cgcatttttg attgtggaca ggcgatgggc
     8581 aatcacaaaa ctggtttttc cttccatcat tgctaagaag gcttgttgga tttttttctc
     8641 tgtcaatgta tcgactgagc tggtagcttc atctaagatc aacatagggg gattactgat
     8701 catcgttcga gcaatcgtca acagttggcg ttgtccatct gatatcttta acccttgacc
     8761 gccaatctta gtatctaatt tttccggcaa acgttctaca aattcataca tataagaggc
     8821 ttttagggca tcattgattt ctgcgtcact tgcttccgga ttcccaaaac gaagattttc
     8881 tcgcaatgta ctgtcaaata gccaagtatc ttgtaacacc attccaaaac ttttacgtaa
     8941 tgtatcacgt tggatagagc ggatgtcatg gtcatcgact gtaattgttc cttttgttgt
     9001 gtcataaaaa cgcatcagca aattaacaag tgtggatttt ccggcccctg tttttcctac
     9061 tatagcaatg gtttgacctg gataagcagt aaaattgaaa tcttcgatca aacgctgctt
     9121 ggggtcataa gaaaaagaaa catgatcaaa agagactttt cctttgatat ttttgggttc
     9181 caaggcatag gcatctggca aatcaggctt ttcttctggc tgatcgatca aatcaaatgc
     9241 tcgttctaga ccagaaaaag cggtttgcag ttgtgtcgtg attccagaaa gttcgataaa
     9301 tggtttagaa aactgacttg agtagatcgt aaagctggaa atcacaccaa cagtgattga
     9361 cgaaccactc ttcaatgcca ataggccgcc aactaaacca atcgataaat aagcaaggtg
     9421 atctacaaaa cgagaagacg ggtttgtcaa ggaagaagaa aattgtgccc gttgtccttt
     9481 ttcatataga tcagcattta gcagttcaaa ttgctgttga ctagcttctt cttgctgaaa
     9541 agctttcacg attttctggt taccgattcg ttcggtcaca aagcccgaga tatccccaat
     9601 aatcctttgc tgtctatcga aatttgtttg tgaagcacga gcaacgatcc aactcacaag
     9661 gaaaataatt ggtgtagaac aaagaacgac caatgtcaga agtggactta gacgcatcat
     9721 aaaaataaac gaaagaatga tgattgccac tccagaaaat aattgattga aggccgcaga
     9781 aattgcaatg gaaatattgt ccatatcatt tgtaaatcga ctgacgatat ttccgtggga
     9841 gttttgatca taataacgaa gtggcaatcg attcagatga gcaaatgcat cttttcttaa
     9901 ttttgcaacg gataagtaag ctaaccgatt gcttaacact tggatgagcc attgactgac
     9961 tacgttgatt aaaacgatac caccgaatag aataagtaaa tggaacagtc gattgaagcg
    10021 gacctgtcct tctccaatca tttgatcgat actttgacca atttgtaacg tcatcacaac
    10081 tgttgcaatc ccattgatga ttcctagtat cacagctagc caaatgtctt ttgcataggc
    10141 agtaaggtaa ggaatgaatc ggcggagcgt ctgcaaggat aattttttta ctttcataac
    10201 tattcttcct cctgtgatgc gacgatttcc tgatattctg ctgattgagc aagtaattct
    10261 tcatgggtac cctgagcagc aatcttacct tgatccatca ctaagatatg atgtgcttct
    10321 tgaatagagc ggacacgttg ggagataata atcactgtac tgccaagata agtctgtagc
    10381 gccttacgca aatccaagtc tgtttgataa tccaacgcac ttaaagagtc atctaaaatc
    10441 agtaatgctg gcttggcaat caaagctctt gcaatcgtca gtcgctgttt ttgtccgcca
    10501 gagaaatttt tcccattttc catgactttt gtatctaaac catttggaag ctgacggaca
    10561 aattcttctg cctgagcgat tttcagagct tcccaacatt ctttttctgt ggcgtctttc
    10621 ttaccccatt gaaggttttc acgaatagtc ccggtaaata gtacagaagt ttggggaaca
    10681 agagcaattt gctgacggag atgatccagc tgccaagaac gaacatccat tccgtccatt
    10741 ataactgtac cggaactgac atcatagaaa cgcggaatca attgtgtcaa agtcgttttc
    10801 ccgcttccgg ttggaccaat gattccaagg actgtatttc gcttcagctg aaatgtaata
    10861 ttttggagag aaagaccaga atcaggagta taacggaagt caacatggtc aaaagcgata
    10921 attgcttcag aagaaaaatc gggagtagta ctgacctcag cttcaagaat cgaattttct
    10981 gtgtctagca cctctttcac ccgatttcct gaagcttcgg cacgtgtgaa gatcacgacg
    11041 aggtttgaaa caacgatcag tgccaaaagc atttgattca tataattgat caaagcaaga
    11101 acttgtcctt gttgcaagct accaatattt actttgatcc cgccaactgt cagcaaacag
    11161 atgatcccga cattcatgat caaggttgta gcaggtgata atagggcaga gatattggaa
    11221 acacgtaaat aattgttcgc tagatcattt gtgcttttat cgaacttctc tgtttctttt
    11281 tttgtctgcg caaatgcgcg gatgacacga acaccgctaa gattctgact aacgtgttca
    11341 ttgagctgat ctagtttttc ttgcactttt tggtagagag gaacggtttt tttgatgata
    11401 aaaaataaaa tgattgagaa aataggcaat aatgctaaga aaaacagacc tacttcccag
    11461 tcgatcacaa aagccatgac cactgacccg atacttaaaa acggtgctct gatcaataaa
    11521 cgaatgacca ttgctaaagc tagctgaagc tgattgatgt cgtttgtcat tcgggtgatc
    11581 aaggtatctg ttccaaaatg attcaactcg ttgtgagaga aagtattgat ttttttgatt
    11641 agctgattac gtaactctgt accaaatcct tgtgaagcta ctgaagcata gtattggcaa
    11701 atgatagcac aaatcagacc aatcacagac atagcgacca tccataagcc catttcaatg
    11761 atttttcctc ggtcattcat ttttagtcca ttatcgatca aggaagccat cattaaaggc
    11821 aggacaagtt cgaagacagc ttcaagaaat ttgaagaacg ggcccaaaat gatttgctta
    11881 cgatagtctt ttgcataacg taataactga atcatatgat tcctcctatt atttatttgt
    11941 ccacaattga aaaaacaagt actaccacat tgtagcttag gaaatttaga atgaaaagag
    12001 aacaagcctt ttcataaa
//
LOCUS       DAZUKZ010000076        11903 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000076 DAZUKZ010000000
VERSION     DAZUKZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.078"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACG8EF_001989"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACG8EF_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFS4947286.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACG8EF_001990"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACG8EF_001990"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFS4947287.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="ACG8EF_001991"
     CDS             complement(2169..2906)
                     /locus_tag="ACG8EF_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS4947288.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="ACG8EF_001992"
     CDS             complement(2997..3524)
                     /locus_tag="ACG8EF_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947289.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="ACG8EF_001993"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="ACG8EF_001993"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFS4947290.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="ACG8EF_001994"
     CDS             complement(4401..4886)
                     /locus_tag="ACG8EF_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFS4947291.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="ACG8EF_001995"
     CDS             complement(5039..5236)
                     /locus_tag="ACG8EF_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947292.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACG8EF_001996"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACG8EF_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFS4947293.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACG8EF_001997"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACG8EF_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFS4947294.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="ACG8EF_001998"
     CDS             9211..9423
                     /locus_tag="ACG8EF_001998"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFS4947295.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="ACG8EF_001999"
     CDS             9425..11104
                     /locus_tag="ACG8EF_001999"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS4947296.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="ACG8EF_002000"
     CDS             complement(11358..11558)
                     /locus_tag="ACG8EF_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4947297.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11851)
                     /locus_tag="ACG8EF_002001"
                     /pseudo
     CDS             complement(11708..11851)
                     /locus_tag="ACG8EF_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
BASE COUNT         3448 a         2571 c         2067 g         3817 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa tttcatatta tcaagagtaa attacaaaag
    11881 aatcatttaa atcaatactt tca
//
LOCUS       DAZUKZ010000077        11839 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000077 DAZUKZ010000000
VERSION     DAZUKZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.079"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..492
                     /locus_tag="ACG8EF_002002"
     CDS             55..492
                     /locus_tag="ACG8EF_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947298.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            587..1471
                     /locus_tag="ACG8EF_002003"
     CDS             587..1471
                     /locus_tag="ACG8EF_002003"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4947299.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1695..4862
                     /locus_tag="ACG8EF_002004"
     CDS             1695..4862
                     /locus_tag="ACG8EF_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFS4947300.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4930..5427
                     /locus_tag="ACG8EF_002005"
     CDS             4930..5427
                     /locus_tag="ACG8EF_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFS4947301.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            5446..5910
                     /locus_tag="ACG8EF_002006"
                     /pseudo
     CDS             5446..5910
                     /locus_tag="ACG8EF_002006"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            5907..6665
                     /locus_tag="ACG8EF_002007"
     CDS             5907..6665
                     /locus_tag="ACG8EF_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4947302.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6751..7491)
                     /locus_tag="ACG8EF_002008"
     CDS             complement(6751..7491)
                     /locus_tag="ACG8EF_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4947303.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7552..8805
                     /locus_tag="ACG8EF_002009"
     CDS             7552..8805
                     /locus_tag="ACG8EF_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS4947304.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9127..9354
                     /locus_tag="ACG8EF_002010"
     CDS             9127..9354
                     /locus_tag="ACG8EF_002010"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS4947305.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9477..9803
                     /locus_tag="ACG8EF_002011"
     CDS             9477..9803
                     /locus_tag="ACG8EF_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS4947306.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9871..10584
                     /locus_tag="ACG8EF_002012"
     CDS             9871..10584
                     /locus_tag="ACG8EF_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS4947307.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10894..11745
                     /gene="licT"
                     /locus_tag="ACG8EF_002013"
     CDS             10894..11745
                     /gene="licT"
                     /locus_tag="ACG8EF_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS4947308.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
BASE COUNT         4039 a         1975 c         2483 g         3342 t
ORIGIN      
        1 gtataatctt aaataaagaa aacaatatca aaaaatgcac gtagaagtag ataaatggag
       61 tggttttgca aatttctttt atttctagaa gttattcacg tatacggatt tgtttttctc
      121 acaaataaaa aggcaggaag tacaatggat catttgtata taaaatatat tcatcaattt
      181 tccgttttgg tgttaggcac actaattata gcggggattg tcgcatttat tattcaaggc
      241 cgtgacatat taacagtaca tcaagagttt ttcagttatg catttggtat tcctctgatc
      301 gctataaggt ctgataacaa aaagtggaag aaacgctatt acattctctt gctatttttg
      361 tgtttttatc tgccacctaa gatttttact ttattaggta tacaatgcct atcattttct
      421 tttatttcct atatcattgt tttaattttc ctcagtttca tagtatggat gttttatctt
      481 gtcaaaaaat aaatgaatgt ttcatgaaca gtacttcatc acagcctaat ttttttcttt
      541 atgctatcgt tatattaaag aaatcaatca gaagggaaag aaagtgatga aaaaagtttt
      601 attagtggta aaccccagtt caggcggtga acaagcaaag gaatttgagc aattagcgat
      661 agcaaaattg gaatcggtgt ttgatgaagt ggtcgtcttg catacaaaaa aagcagggga
      721 tgcaaaaaac tttactcgcg aagcggctac ggaagggtat catagtgtgt ttgtcatggg
      781 cggagatgga acagtcaacg aaggaatcag tggaatcgct gaacaagaac atcggccaaa
      841 tttcggattt ttcccattgg gtacggtgaa tgacttggct cgagcattag ggataccgct
      901 agaacctgag gaagcgatca atcatttttc gattgaatca gtcaagccat tggatattgg
      961 aaaaataaat gacgactatt ttatgaatgt cgtcgcaatt ggctctatcc ctgaatcaat
     1021 caatgatgta gatgcggaga agaagacgaa atttggaaaa ctagcttatt tcatgtcagg
     1081 aatcaaacag ttagcttcca cacaatccta ctcgtttcac gtagaagttg atggaaagaa
     1141 ggaagaaatt gagagcagta ccttgctgat aggactaact aactcagtcg gcggttttga
     1201 aacattactt ccaaatgcaa aagtggatga tggtaagctc catttggttt atttaaaaga
     1261 ttcttctttg atcgatacgc ttaaaacatt gccagaccta ttaaaaggag tagatgaatc
     1321 tacgaaaaat ctagcctacc ttacttgtga gcaattagtt gtgtcattag ctgatgagga
     1381 agaattgacg acgaatgttg atggagatga aggggataaa ttacctgtga caatcaaagt
     1441 attgccctca catttacagg tttattgtta aaatggaggt tgtgaccgag gagttggcgg
     1501 gagaacaccg cttgttcggg ataagggact gtgacaagac ttttgtcaca gccttttttt
     1561 tgtacaaaaa agaaatttac acctaataga aaaatttttc cataaaaata catattttaa
     1621 atatgaaagt acaattggaa atagattaaa acgataaaat aaaattgtca aaacgtcaga
     1681 gaggatggtg gagaatgaat caatgtgtaa aaaagctgat tgcgtcgatt ttgtttgtct
     1741 tccctctctt gatggtgaca tccgtttttg ccgatgtaga gaaaacaaac gaatcaacgc
     1801 aatcagagaa tcaaagtttt gacaagacta ctttttcaga agaatcgatt acttctgaaa
     1861 ctacagaatc tcaaagtgag gcttctacta ataaaaggca agaagaaaat aagacaagtg
     1921 agacctcaga agtagctgaa caaacgtctg atagcagtga agcggcactg gcaagagctg
     1981 tttcagcgga tgggtacagc gaagctgcca caacagaaga actggcgcag cttctcgcag
     2041 acgaatcagt aactaagatc cgactgattc agcctttgac tttagacagg gaattagaga
     2101 tcaaacgaga tatcgttatt gattttggtg gattcgctca caatttcggc acacaccata
     2161 tttatatcaa tgagatacct cagtcgatcg agttccagaa ttttaaagga acagctgcac
     2221 atccaggtgg attgacacca aatatcgatg gaaatgccat tatcattgct tatatgaccg
     2281 atttttgggg aagcagatat cattttacgg gagaaataaa atttaccggg acacttgatt
     2341 tgtatgatgg gtccaaatta ggactgattt acgctcctcg tgcaactgtc acattagatg
     2401 gtgtttccgg tgtgttggat gtccaaccag tgaaggaagg actgaatgcc gctccgggta
     2461 aagcctattt ttgtagaagc tatcaattga atgtgatcaa tggtagtcaa ctttatggac
     2521 cgtatctagg gaaattctac ggttttctag attcaggaga tgagactggt agtggaaaaa
     2581 cagcgccagg aatccatatc ttaagcggtt caaaggtctc tcttgattat gatcgggcag
     2641 atggcgttag cgatgaagga gaggcagtcg atacgctacc tggtaatgtc atgtttgaag
     2701 tcagtggttc gggaagcgaa ttttcggtaa atacaaagat caatattacc aatgacaaca
     2761 atcgcggaat catccaaatg agaggttcag gatcgaaagt cactgtatcg aatggtggga
     2821 aaatcaccat caatacagca actacgggcg gatttcgctt gcaaggagat tctagtcaaa
     2881 tccatgtatc aagtggtgga caaatctcgg tcaaaatggc tggagatgac gatcaacccg
     2941 ggaacaatgg gatgcgtttt gtaggaaaag gcctttcttt gatcgtagaa ggagccggat
     3001 cagttatcga catcgataag cagagtgggc gttccagcgc agttcgtttt gagaatggta
     3061 cgcaaaagct gacagtaacc aatggaggct cattgagggt aagaaataca ggtgatggca
     3121 aatcttacgt gaacagactc aatcaaggaa atcaagcgat ccaattttat gacgcttctg
     3181 gttttctaga aagtccaggt tcagctgatt ttacagtcac tggagaaaaa tcaaatatcg
     3241 atatacgcgc agattccgga gcaactgtag atacaactgg agatattgat ttaaatttta
     3301 cggcaggtga aaaaacttat ttagtcatgg tcgggaaaac aggggaagat aagaccggca
     3361 tcatcagtgg ggatgtgttg aatgtggact tagaatctcc tacctatttt gatttccaaa
     3421 acaaacggat tggaagcgag accaatggca taggcggctg ggtattccaa ggaaatggag
     3481 attcttcttt aaagatcgcc cattcagaaa tcgctttatg gcgaaaaaca ggcgcaacat
     3541 tcgataactt tgaaaaagac ccggattatt tttctgatat gacagactta tcgatcaatg
     3601 ggaccgactt aggcaatttc gtatcaagcc ctagtgcagg tttaacaaat gaatttgctc
     3661 agacaggtgc cggaatgaaa aattataatc gaatcagtgc caataatcag ctcccgatga
     3721 ttgaatcctt aagagtgcca acgaatgcag ataaaaaaat ctatgggcat gtcgttgttc
     3781 ccgaaggtgt cgaggcaaag ccaagagatg cttggacagg cgaagtagaa gtgactttac
     3841 gtattactta cgcagatacg acgaaatcat ctgtagaaat tactgctctc acgcaaggac
     3901 aaacagatga aaatgaaggg gcaggatata acccatgggg agaaggcgaa caaggcggct
     3961 tgttcgaagt tcaagtaccc aatgatgaga gattaaaaac ggaggatcaa gtgcaagttg
     4021 ttgctgctaa aaaagtaaga ggtggccaag aagtgacaga actggactct gccaaaacga
     4081 cagtcgatgt gattcctcct gaacctgcta atttaagtac cacattgatc aatgcagctt
     4141 ctcgcacgat cacaggtaca ggtgaagcag gagccttggt ttcattgaaa aaaaatcaag
     4201 ctgttttgtc agaaacaact attgatgata caggggaatt tgttctttct atcccagaaa
     4261 atcaactttt tgcaggagat gtattagaaa ttgtattaaa cgaccaagca ggagaagcta
     4321 ctgctaaggg tgtgcagaac aagccaagta ccaacgatga aattgggaat cacaatccaa
     4381 gtgatacgcc agttgtttat catgatgccc cgccttttgc acctgctacg aagatcgtag
     4441 tagaaggtgg tctctcattt cttgcccctt ccaaactcga ttttggtcag atcaaagcaa
     4501 ctggtttgac acaagaagca tttggccaaa tggatataca ggaaaaatta tggatttatg
     4561 atcagcgaga aacgaaaaat ccgtggatct taacgataaa attatctcag cctttcacaa
     4621 atgatgcagg attttctatg caggatgtcc tatatttcaa gaaaggagag gagtatcaat
     4681 tagtgaatga agaagaactg gaaattgtca gaggacaaaa ttcaacgaat gtgcaagcag
     4741 attattcaga atatttaaat aacggtcggg cgtttaaact tgtcttaacc aaagaaaatc
     4801 agatagctgg taattactca gcagagataa catggacatt agcagatgcg ccgacagaat
     4861 aaagatcaag gaggatgatt gatgaatcga atatggaata aaagtcatcg aaacaactgc
     4921 tctttactta tgatccttct ccttggattc atcctttggc agacacctgt ccatgcctcc
     4981 gaattcaatt ttgcggtaga ttttgaatta ccggctaatc agattgataa gaacaaaaca
     5041 tatataaatg taaggatgga gcctgggaaa acacaggaaa tcccctacac ggtctttaac
     5101 cgaacagata aatcagtaac agttgagaca ttgatccact ccgctacgac gaattcaaac
     5161 ggtgtgatcg aatatggaga atcaaaaagt aaatttgatg aaacattaag ttatgatatg
     5221 aaaaacttag caaaaacagt gggagaaata actgttcctc ctcaaagtgc ggtcactgag
     5281 acactttcac tgaaagcgcc agaaaaagag ctggctggtg tggtcgcagg agggatcacg
     5341 tttcaagaaa aaaaagagaa cgtttctaac aaaagtacag ttggtgtcgt caatcagttt
     5401 tctcaagtga aagcaatctt gatttgaaat agcgaaaaag agtcttcccc agatttgaaa
     5461 ttaaaaaaag cttatgcgga tcaaagcgat ggaagaaatg tgattaaggc gaatcttcaa
     5521 aatccaaatg cttcttatat gttcggcttg actacatcgg ttgaaatcta caaaaaagaa
     5581 acaaaagagc cttacctttc aataaaaaaa gaaaaaatag atgtagcacc caatacaaat
     5641 tttgatttac ttgtcccatt agaaggaaaa aaattagctc cgggacatta tcaagcggtt
     5701 gtgaagggtg cttggaaaga aaaacgctgg aatctccata ccgattttga aataactgat
     5761 gatgcagcgg accaattgaa tgaaaaggat attgatttaa aaaatcagcc agaaacattt
     5821 gactggcgat ggctgatact tgttggagtc attcttattg gcttatttgc tggattattt
     5881 attttaaaag aaagaaggaa aaagaaatga aaaaaacaat tgcattaagt accgttctag
     5941 ctggaagtct attattatgt acacaaagtg tttttgctga gaataagagc tcagccacta
     6001 cagaaggaac gatttctgtg accaaaccag atgctgcaaa tcctgaagac gttgttccag
     6061 ttcctcctgt tgatccaaat gatcctgata ctccttttac acctgacgaa ccaaatcagg
     6121 gaacatccgg attactgacg attgaccaag ctccagatct tgattttgga acaatcaagt
     6181 taggtacagc aaaaacagta tatgctgcat tacaaagcgg aacggacagt actggagcac
     6241 aaacacaagt acagaatttc gtacaagtaa cggataaaag cggaaattat gccggttgga
     6301 aattatcagt caaacgaaca gaatttattg gtacaacatc tgatacgaat aaactaactg
     6361 gttcaaaggt gaaattcaaa aacgccgtga ttcgaaatag cgcaactaat agcagtggaa
     6421 tgccatctac cgtttctgga actggcgctg aaggaatcga aatcccagca gatcaggcag
     6481 tagatctagt aactgcagga gcaaatgaag ggattggcac atggatttat tcattaggag
     6541 agaatgtgga acaagggaga caatcagtgg aattatttgt tcctgtatcc aattatgctg
     6601 tagacaacta cgtttcaaca ttcacatggt cgttaacaga tgctcctaca gaagaggaag
     6661 cgtaaaatca tatcaacgat ttcagggagt ggagccgatt caaggcccac tccctgaaat
     6721 ctgctatgct tacgtctcag ctcttattca tcaactcgaa gcagtttttt ggaagtcatg
     6781 gaaacgttcc tttgtctttt caaagatctt tcgaccaacg agtaaggagt caccgttgaa
     6841 aaattctaac acataatgag cagaatcaat tttctgcaat tgcgataagt tgatacagta
     6901 agattttaaa agtaaaaagt aagcatactt ttctaactct gtcttcaatt gttttaaaga
     6961 agtattcact aatttatctt ctgtagttgt tttgaccata acttggtttt tcaaaccttt
     7021 gatactttca atatataaaa tttcccgagg atacaggacg agacttccac ctttttgatc
     7081 aataaagatt ttatcgacat ttacgatctg ctgttctttc caattgatct gttctagcac
     7141 ttgacgaatt tgttcagata aattcctgtc ttttctgata tatccaatcg gaaggatttt
     7201 ccgattgatg atctctactg caaaattcgt ataagcggtg aaaaaaacaa tattactatg
     7261 tgcatttttt ttacgtattt gttcggcgac atcaatacct gtgagtgatt gcttcaagtc
     7321 gatatcgata aagaaaacat cgtgattatc aatagtgccc aactggatca actgattgat
     7381 ttgagagata gaagtgagct gcacgatatt ctcgatatga tgattgacca attctctgat
     7441 gatccattcg ctgtgaaact ggttatcttc tatgataaaa tactttttca tatgcaggct
     7501 cctattaaaa aaatatgtgt acattttgaa acaaaacaat ggaggaataa gatggtcgca
     7561 acagtgacac tttatttgac ctttactttg attttagatt taggtatcta tttattcttt
     7621 tcgaaacaac tgctagtaaa aaacagcttt ggttgtatag ctttgatggt ggtgacactt
     7681 ttacttccta tccctaaagt gagctttcct tacctagttg gcttattatt gatgtttctt
     7741 ttttatttta ctacgaaaaa cctgtttatc agctgtttga tcagtaatct tttttttgct
     7801 atcactggac tcagcttttt tctggttttt gatctgatgg attatttagg aaagagacat
     7861 cttggaacag caagtttttt tgcactactt atcgcattta ttttctatct attagtaaaa
     7921 agtatagaca aacagacgag atttctcaac ttgcttttta cttatccaac tgaacactat
     7981 tttggaggta tttgtatttt attaagctgg ggcgtgattt acttggtatt gggaagtgtt
     8041 cacttccgct catctaatta tctgcttcta tctcttttgt cgatagtcat cagttttttt
     8101 tctttgtttt tttccattat gttgatcagt ttccaagtaa aagaaagaca acatcaggaa
     8161 tcgcttcaac tgtatcaaat aaatgaagaa tattaccatc atctagagga attcaaacat
     8221 gattataaaa gtctactgtt ttcattaaaa attactcttc aggaaaataa aaaagaaacg
     8281 ttggaatttt tagaaatatt agaagatcat tctgcagcta tttttgaaaa accgcaaata
     8341 caacagctaa caaatattgt atccccagct gtcaggggtg tctttttaaa atttctagaa
     8401 aaagcagaat cggagaaaat cccgatccaa gtagttattc ctgtcgaagt gaaaacaatc
     8461 ccgattgatc tggttgtttt tattcgctgt ttatctattt ttatttctaa cgcattcgat
     8521 catcgtgtag ccgatcagag tccgattacg atcaatctgc tggaagaaaa agagggatca
     8581 cgttttagta tagcgaacaa ggcaaatcca acgaatttga cgctatcgga gatggtcaaa
     8641 agaggaaaaa cctctaaaaa aaatggcggg ttaggtctat acagtgcgaa aaaaatgttg
     8701 gatgtttacg aaaatgcaga actaaaaatc gaattttcaa aacagaatca ctattttttt
     8761 gtagaaatgt atctggcatc tagaaagagc aaatcagcct actgacttac tgtcaaagga
     8821 taagtttgag gaaagtctac acggagatag agacaacaac acagcatatt caaaagaaac
     8881 agaagcgtga tgaccgttgt cacccttctg tttcttttgc tatgtagtta aacaaagtag
     8941 ttgacacttc cgctacttag tattagtacg aaagtatact tcatgatact aagtagataa
     9001 gaagtgaaaa gcggaaccca cgatcgtcaa aatgctaact gctttgctta caccagataa
     9061 agggaagagc aatatcgctt attttaggcg tggagaagca agcaaatccc tacgatcaca
     9121 aaaaaaatgc tgacccagca gttaagagag ttagaacaag atgaaagtat ccatcgaaaa
     9181 gcctataacc aagtcccgcc taaagtagag tactcgctca ccgaagaagg aaaatcatta
     9241 agagagattc tagtagcgtt gtctgtttgg gaagaagaac gtatcgaaaa acaaaaagcc
     9301 tcaagggaag aagacacgct actgcataca catggtggct acctaaatta ttgattttct
     9361 atgacattta ttttaaataa aagaaaacag gagttttttt aaaagcatca tctatttttt
     9421 atgtaaaaaa atggtatgat taatcaagta agaagtgatt ggaagaaggc aagcaaatga
     9481 atagtatgga aaaagtcaac cagtttagag atgaacgtaa ttggcgacaa tttcataacg
     9541 aaaaagattt ggctatctct atttctttgg aagcatctga attgttagaa ctttttcaat
     9601 ggaaaacgcc ggaagaagta aaagaaacac aattagaacg aatcaaagaa gaactggcag
     9661 atgtattgat ctattcatac atgatggcag acaaccttca cctggatcta gatgagatca
     9721 ttgaagaaaa attgatcaaa aataatttga aatatcctgt agaaaaaagc gcaggtgtac
     9781 gaaaaaaata cacagagttg tagaaatgtg tcattcggct gttggaaaga gcaaaagaaa
     9841 aacagtaaaa tgagtctgga gggagagaaa atgaaaaaaa aaggaaaagc aaaaagatgg
     9901 ctagtcaata tcctcttgtt cctcttgtta ttagtaggac tagctttgat tttcaatgaa
     9961 cagatcaagg attattttgt cagagagaca ggagataaat atgctatagc gaatgtaaca
    10021 aaagaagatc tgaaaaagaa taacgataaa gatgtcagtt ttgactttga tgcggtagaa
    10081 ccaatgacga cagaaggggt catgaggtcg caaatgagag gaacagatct accagtgatt
    10141 gctagtatcg cagtcccttc agtttcaatc aatttaccgg ttttcaaagg cttggataat
    10201 acgtcactac tttacggagc aggaacatta tctccagatc aagaaatggg aaaaggaaat
    10261 tatgccttag caagccatcg tgcaacgaac cctgaattgc tgtttacacc gctagaaaat
    10321 ttagagatgg gtgccaagat ctatttgaca gatttagaaa atgtgtatac atacaaaaca
    10381 ttttttaaag aaaaagttgc tcctacagat acccaattac tgaatgaagt cgagggaaaa
    10441 gaaattgtca cactaattac ttgtggcgac atggacgccg tcacgcgact agttgtacaa
    10501 ggagaactgg aaagtgttac atcgataaaa gatgcgacag atgatatgag atctgctttc
    10561 aatctagaga caaaaacatt ttaggatact gggacataag tcgttacaga ttaaaaacag
    10621 ccaaaggata tgaatcgata aggaaaaacc aatttgctga aaaaaagaga aacgcaaaga
    10681 ggtgcatctc tgatttggtt gttttctaga tagacttgtg ttccagtttt tttatttttt
    10741 atatgaaaag gcttgcataa ataaaaattc gatggtatga tgattttgaa taagaaatgg
    10801 attgttactg tacggcaggc aaaaccagaa ttcagctaag aaacagtagg cgaattctag
    10861 gtttttttat ttttcttgag agggcgaagc aaaatgaaaa ttgataaaat tttgaataac
    10921 aatgtcgtca tttcgaaaaa tggattcggg gaagaagtcg tttgtatggg aaaaggacta
    10981 gcttttcaaa aaaagattgg ggacgagatt tcgccagaag cggtccaaaa agaatttgtt
    11041 ttaagagatt cttttgctaa aaaccaattc cagcagctga tggctgatat tccggcagag
    11101 gagatcgaat gggtcaaaca agtagtcgaa ctggctgaag ataaactaaa agttgaattt
    11161 tcgcctaata tttatctgac attgaccgat catcttcatt atgcgatcac acgagcaaaa
    11221 gaaaatatcc aactgcctaa tccattatta tttgaaacaa aaaaatttta tccgaaagaa
    11281 tatcaagcag cgaaagaggc tcttttgttc gtgaaacaaa agacgggtgt ggaactttct
    11341 gaggatgaag ccggttttat tgcctttcat tttgtgaata gccaacaagg aaacgaagac
    11401 atgcaagtga cgatgaccgc taccacaatg gtaagagata tactgagtat catcagtaaa
    11461 ttcttcggca tcgtgttcga tgaagaatct ttgaattatc agcgtatcat tactcatctg
    11521 cagttttttg cccagcgtta tctcaaaggg gaaggatctg acgagcaaga tgaatttctc
    11581 tacgcattag ttcaaggaaa gtatccaaaa gcgtttcatt gtgtcgaacg gatcaatgag
    11641 tatcttcgac aaacgcaaaa acaggaaatg gggatcgcag agcagattta tttaacgatt
    11701 catattcaaa gagtagtaag tgagaaaaaa agcatccaat cataaggatt gttactgtat
    11761 ggcaggcaaa acctggattg aaaaaatagg ttcacgaatg tttgtgactg tttttttcaa
    11821 tctgggtttt tatttttta
//
LOCUS       DAZUKZ010000078        11609 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000078 DAZUKZ010000000
VERSION     DAZUKZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.080"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8EF_002014"
     CDS             6..305
                     /locus_tag="ACG8EF_002014"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4947309.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8EF_002015"
     CDS             378..1739
                     /locus_tag="ACG8EF_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS4947310.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8EF_002016"
     CDS             1761..2021
                     /locus_tag="ACG8EF_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947311.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8EF_002017"
     CDS             2035..2787
                     /locus_tag="ACG8EF_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFS4947312.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8EF_002018"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8EF_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4947313.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8EF_002019"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8EF_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFS4947314.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8EF_002020"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8EF_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4947315.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8EF_002021"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8EF_002021"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS4947316.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8EF_002022"
     CDS             7161..8558
                     /locus_tag="ACG8EF_002022"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4947317.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8EF_002023"
     CDS             8639..9079
                     /locus_tag="ACG8EF_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4947318.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8EF_002024"
     CDS             9227..9553
                     /locus_tag="ACG8EF_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4947319.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8EF_002025"
     CDS             9554..10303
                     /locus_tag="ACG8EF_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFS4947320.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG8EF_002026"
     CDS             complement(10383..10838)
                     /locus_tag="ACG8EF_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4947321.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACG8EF_002027"
     CDS             complement(11265..11540)
                     /locus_tag="ACG8EF_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947322.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3665 a         2013 c         2360 g         3571 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttgc ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagg
//
LOCUS       DAZUKZ010000079        11547 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000079 DAZUKZ010000000
VERSION     DAZUKZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.081"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..695)
                     /locus_tag="ACG8EF_002028"
     CDS             complement(150..695)
                     /locus_tag="ACG8EF_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS4947323.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(707..1399)
                     /locus_tag="ACG8EF_002029"
     CDS             complement(707..1399)
                     /locus_tag="ACG8EF_002029"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFS4947324.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1418..1702)
                     /gene="macP"
                     /locus_tag="ACG8EF_002030"
     CDS             complement(1418..1702)
                     /gene="macP"
                     /locus_tag="ACG8EF_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFS4947325.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1715..2278)
                     /locus_tag="ACG8EF_002031"
     CDS             complement(1715..2278)
                     /locus_tag="ACG8EF_002031"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS4947326.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2506..3168
                     /locus_tag="ACG8EF_002032"
     CDS             2506..3168
                     /locus_tag="ACG8EF_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFS4947327.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3292..4488
                     /locus_tag="ACG8EF_002033"
     CDS             3292..4488
                     /locus_tag="ACG8EF_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFS4947328.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4589..5965)
                     /locus_tag="ACG8EF_002034"
     CDS             complement(4589..5965)
                     /locus_tag="ACG8EF_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4947329.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(6002..6868)
                     /locus_tag="ACG8EF_002035"
     CDS             complement(6002..6868)
                     /locus_tag="ACG8EF_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS4947330.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6957..7982)
                     /locus_tag="ACG8EF_002036"
     CDS             complement(6957..7982)
                     /locus_tag="ACG8EF_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4947331.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(8000..8674)
                     /locus_tag="ACG8EF_002037"
     CDS             complement(8000..8674)
                     /locus_tag="ACG8EF_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4947332.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8858..9751)
                     /locus_tag="ACG8EF_002038"
     CDS             complement(8858..9751)
                     /locus_tag="ACG8EF_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4947333.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9825..11522)
                     /locus_tag="ACG8EF_002039"
     CDS             complement(9825..11522)
                     /locus_tag="ACG8EF_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4947334.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3433 a         2523 c         1979 g         3612 t
ORIGIN      
        1 aagttgttga taataaagag atttattaac gtaaaaagaa taagtttttt taaaataacg
       61 tataaaaaag agcataaaac aaagtcgttt gttttatgct cttcttttat tattttgtgc
      121 tttttatttt atcaaatcac tttgtttttt tataaaattt ctgcgcctag tgtggactca
      181 aaatgtctca atgcccattc atgtccaacg ggatcgaaac ttgccacggc atctttgaaa
      241 atatggagtt tgtatcctag attatacgca tcgactgctg tatgcaacac acagatatct
      301 gtacatacgc cggtcaaata aatatctgtg atctggcgtt ctcgcaaacg gatgtctaga
      361 tctgttccac taaaagcgga ataatgccgt ttgtctatcc aataaacatt ttcctcttgc
      421 tcatagtctt ggtataaact ttttaaagag ccgtacaatt cccgtccacc cgttcctatc
      481 acattatgcg gaggaaataa gcggttctcc ggatgaaagg aatcttttgg atcatgcgca
      541 tcaatggcaa aaacgacaaa atccttttga tcaataaact tctttgtatg agcagtcagc
      601 tttttttcaa ttacttgtcc tgctgtccca gtagtcagct ttccatcctc tgcaacaaaa
      661 tcatatgtgt agtcaatcga gatcaatgcg ttcatcttag tcctccttat acgagatgtt
      721 tgacaaattc gatcaccatt tctgctgatc gtttgcctgc gtcttcgata aattcgtcaa
      781 agctttgtgt cgccgagtga tctgctgtgt cgctcattgc acgtacgatc aaaaatggga
      841 tattgaactg acgagctgtt tgtccaacag cggctccttc catttcgcaa gctaatgctt
      901 cagggaaatt cgttaagatt tctttgattt tatctggcga gtcgacaaat gtatcacctg
      961 taacgatcaa tccttctttt gcgttcaaat tcgttgcttt tgctgcttta accatttcac
     1021 tgcgcaaata tgtgcttgct tcataatata gaggcattcc tggtaattgt cctggtttgt
     1081 agccaaatcc tgttgcatct gcatcaaaat aagctacttt gtctgatata acgatgtcac
     1141 ctacttgtaa gccttcaccg attccgccag ctgaacctgt attgatcacc atgttcacgc
     1201 cgtattgttg gatcaacaga cttgtggtaa tagatgctaa gacttttcct ataccggaac
     1261 gtacgacgat cacttcgtgg ttacctaatg aacctgaaat aaataaagcg cctgcgcgtt
     1321 cccatgataa tggttcgctt aggttctctc tcaaaatttt tacttcttct tccattgcgc
     1381 cgataatacc gattttcatc tttttaattc cttctttcta tataaatgcg attgctaaaa
     1441 aaaccacaca taacattagg ataacaataa taagagattt catcaaaaaa gagttccatt
     1501 tggtcgtctt ttcccgttcg ctgatccttg ttttcgtgat tggtttattc tttttgcttt
     1561 ctttacgata aaagctggca atcttttttt cttcttgctg ataagctgtt tctgcttttc
     1621 gctgtttttt taatgactcg cttgcttgtg cttgttgtcg cttgcgaagt tcgcttcgcg
     1681 taataagcgg tcctttggcc ataagccgat ccctctactt tcctttggtc aatagttccc
     1741 agtattgaat cgcataaatc gtctttgcat cacaaatcaa ctgatctttc atcgcttgtt
     1801 gcgcctcttc aagagttaga tgatacaact ccagtacttc gtcttcatct tgtgctaaag
     1861 gattctcgac cttctctacc ccttgtgcat gataaatatg aaggacttca tttgcaaaac
     1921 ctggtgaaag atacatcgaa gtaagatggc tcagcgactt cgcacgatag ccagtttctt
     1981 cttctaattc tctagctgct gtcatttcgg gattttgtcc ttctcctgga tcgatttttc
     2041 cagctggaat ctccaaaatc actttttcta aaggttttcg aaattgtttg acaagaagca
     2101 gacgatcttg ttcgtcaaaa gcaatgatcc cgacaccacc aggatgaaat accagttctc
     2161 tcttcgaagt tcctccatct ggcaaacgaa cttcgtctac agcaacatca ataatttttc
     2221 cgctatagat ttctttgcga ctgattgttt tttcttcaaa ttgctcattg ttcagcatat
     2281 cttctcctgc tttctttgta atcataactg cttttttttc atttttcaat ttttctcaaa
     2341 aactccttat tagtttacca tagataaacc ttagattttc tctaaatata gaaaatcctt
     2401 ctttagtccg atactctttc tagtagtccg tatgctaaaa taagtatgta atcgaataac
     2461 gaaaagaatt caatactcaa cgataaatga catggagggt ttattatgat aaaacgaaat
     2521 tggccgtggc ttctagcatt ggtcttgcta tttctctttg tccgaaacaa tagtttttct
     2581 tttgttctag gacttttatt gataggtgta gttttagttt tgtggggact ctttggttcg
     2641 cgcaggaaaa agggaaaaca agaagaaatt cctgcactga ctgacgaact tgaaactcac
     2701 tatgaaaaaa gcggtatgac agcgaatgaa atttcatttt ttcgacaaac aatgaatcaa
     2761 acaaaaaatg agatcaatca attgcaaatg aatatgcagc agacagccaa acttaaagca
     2821 atcgatctcc gccatgatcc agtcaaagca gcgaaagctt tattcaaaga attggtgaaa
     2881 gaaccaaacc gactgcatga agcaagtcag tttctttata cgcatctgcc gaatctagtt
     2941 gatttgacaa ataagtataa tgagatcaat gaccatgaga tcaaaaacaa acagacctat
     3001 gaaaaactag aagaaagcgc gcagatcatc gaccagctgt cagctttaat cgcacaagat
     3061 tatcaaaaat tcgttgctga ggatctagat gatatcgacg tagagatatc tgttgcgaaa
     3121 cagagcttaa agcgtgataa taaatatgaa ccagaacaaa aggattaact cttttgttca
     3181 taaaagaaga ctcggaacat tcagatgatg tctggctgtt tcgagtactt tttctaaagt
     3241 agagacattc atgtaagaat cgagaacaga ttcaacaagg aggaattttt tatggatcat
     3301 aaaccgactg aaacaaaaga tgttacacca gttaacgata cacttgaaga tttattgaac
     3361 aacccgtttt caactccaac agatacgctg accacttcac aacaagcgga gattgatcag
     3421 ctccaagagc aacagacagc tgctcgttta attgacaaac taccggcgga acgccaagaa
     3481 caagctaaac agctagcagc aaaaatcgat gctaatgatg cacagtctgt catcagctac
     3541 ggatcggctg cccaagcaaa attaagtgaa ttctcacaat cgatgttgaa tcatgtgcaa
     3601 gcacaagata ttggtcctgt aggagattcc ttaactgaac tgatgtatcg tctccaagaa
     3661 gccaatcctg acgaactgcg tgccggagaa gggaacatct tccagcgtgt cttcgggaag
     3721 gtcaaacaat cgatctatga agtgacagca aaatatcaaa agattggtgc acaaattgac
     3781 aagatttcag tgaaattaga taaagaaaaa gacggtctgc taaaagataa tctgatgtta
     3841 gaacagcttt accagaaaaa caaagactac tttgatgcac tgaatattta tatcgctgct
     3901 ggagaattga aaatggaaga acttcaaaca acgatcattc cagaagcgat gaaaagagca
     3961 gaagaaactg gcgatcagat ggatgttcag attgccaatg attacacgca attcctagat
     4021 cgtctggata aacgtacgca tgatcttcgc ttagctagac agatcacgat ccagcaagca
     4081 ccacaaatcc gtttgatcca aaacacaaat caggccttgg ctgaaaagat ccaagcatct
     4141 atcgctacag ccattccgct gtggaaaaac caagtcgtga tcgcattgac cttgcttcgt
     4201 caaaaagatg cagtaactgc tcaacgccaa gtctctgaaa caacaaacga cttattgaag
     4261 aaaaattcag aaatgctgaa aatctcagcc atcgaaacag cgaaagaaaa tgaacgtgga
     4321 atcgtggata tcgagaccct tcaaaccact caaaatgatt tgatcgaaac aatccaagag
     4381 acattgcgta tccaaaaaga aggaaaagaa aaacgtcgtc atgcagaagt agagcttgga
     4441 cacatggaag aagatctgaa acaaaaactg ttggatttaa cccaatagat cacctttcgc
     4501 ttgttactaa aacaaacgcc caacaacagt ttattacaac tgttgatggg cgtttttctt
     4561 atctttcttc taacttctcc attggagtct atttctcatt catcttgctg tgtccatagc
     4621 catagccaaa ataaatgatc agtccaataa ccattacgat accgaacacg atccatgtca
     4681 cagtttgcag acgaagcatc aatacaatac aagtcaagat ggagacgatt ggcaatagcg
     4741 gaacgaatgg cactttgaat tctccagctt ttggtttgcc aaacattttt ctcaacttca
     4801 gtaagcctag actaacaagc atcaaatagg cgagcgccat gatattcgtt agttccgcta
     4861 atacattcaa tgggaaaaat ccggcaaaga atacagctgc cacaccagcg gcataagtcg
     4921 cttttttcgg tgtacgatgc ttttgatcga tttctgtcat aaacttagga agtaagccat
     4981 ccttgctgac agcaaacagt aaacgagcaa gtgaatacat catcgagatc gttaccgtca
     5041 gtagcgttaa aattgcccct acagcgatca ccgcgcccac tgttccatgt cctacatacc
     5101 tcattgcaaa agccactggg tcattgactc ctagttcagt atacggaacg attcctgtca
     5161 agatcagcgt cacgatgatg taaaggacag tagcaatcag tatcgaacca atgattcctc
     5221 ttggcacatc tttttgcggg tttttgactt cctcggctgc cattgcaacg gcatcaaatc
     5281 ccaagaaagc aaaaaacaca agagctgcac catctagcac accggaaaaa ccaaaaggca
     5341 tgaatggctg ccagttatca ggtttaacga aaaatattcc tacaatgata aacaacgcga
     5401 tgataccaaa tttgacatat accatggcat tattcaaacg taatgctttc ttggcttcaa
     5461 gcgacacgat gtaagtgacg aatatcacta cgagcaccgc aatcaagtca atgtatgtac
     5521 cattttccgg attgtacccg ccagttaatg cttgtggaag ttcggcccct aaacttttca
     5581 aaaaaccttg catatagcca gaccatccgg aagcaacaga ggaaaccgct agtaaaaatt
     5641 ccccgatcaa caaccaccct gtcagccagg cagcaaattc accaaataca acatacagat
     5701 aggcatatgg cccaccaatc actggcactc gcgaagcaaa ttctgcgaaa cttagtccag
     5761 acaaaatgac aacgattgca gctaagacaa atgaaagtga cagtgctgga ccagcatttt
     5821 gattggccgc aacccctgtc accacaaata ttcccgtacc gatgatcgca ccaattccaa
     5881 gcatgatcaa atcagacgtt tttagttctt ttttcatctc actcgtatga ttgagattga
     5941 tctctttctt acgaaacaaa tccattgaag ttcctcccca ttttaggttg gaaaacagcg
     6001 attaaactgt tttcatatac cctaaaagga taccacctaa aatgactaaa aggcaaccaa
     6061 caataacata aatgagctct tttttcgttt ttctttcgcc aaggatgaaa atcccaccta
     6121 aagtagagat aatgatcccc atttgtgaaa gtgagaagcc aacagctaaa ccaagtgatt
     6181 tgactgttag aagcatgcag atgtttccta agccccataa taatccacag atcatgtttt
     6241 tccacacaaa ctgatcgact ttaactttac ggaaagcaaa gaagcttgcg cctaagatca
     6301 ttccgatact ttgaggtaag ataattgaca tcgcatccaa attcgcccag ttgacgatga
     6361 tggtatatac accataacca atcgtagaga agatcaatgc tctaaaaccc ttaccaaaat
     6421 caagcatttt attatctgta tctatttttc cttcgtcatc ttgtcgggca gtcaaatacg
     6481 caccgcttac aagaagagct aaagcaataa taccgtatac ataatctttc gtttgtgtcc
     6541 attcatggaa gaaaacggct cccgcaatcg tattcaaaat taattgaaaa ccagtagaca
     6601 atggtagccc tacagaaaca cccatgtact tcattccatg gaactgtcca ttttgtccaa
     6661 cactccaagc taagccggat aaaaatccga tcaagaaaat ccaaggggtg attacaggac
     6721 taacaacgaa aaagacaact aaagaaaaca gaaatgcacc gatcgtcatt cctagtgttt
     6781 gttgattggc atcgccacca attttaccac ttactaaacc aatactaccc catgccaaca
     6841 tagggactaa tgctattaaa atttccataa ccaatctcct tttatttcac atgataagaa
     6901 acttatcagc gaaaacgtta acaacacaag cacttttttt cgtttctaac gattttctaa
     6961 cgtttttcat tataaaaatt taagattggg aaaaataaag tcattgttgt cccttttcct
     7021 tcagttgatt cagccgtcaa atggatacct aatttttcgg ccagattttt tgctaaataa
     7081 agtcctaatc ctgttgaatg tgttttgcta agtcgaccat ttctcccagt aaatccctta
     7141 tcaaagatcc gtttcaagtc ttccgctggg ataccgatcc ctgaatcttt aactgaaagg
     7201 ctgactcctt gcgcttcctt ttctatgctc acaatgatgt ttccgtgatc cggcgtatat
     7261 ttgatggcat tactaagcaa ttggttgaag ataaaagcga tccatttagc atcggtcaaa
     7321 acagtatgat cctcaccaac gatctcgtag cgaatatttt tttggataaa ataatttgcc
     7381 tgactgcgta tcacaggctg aatgattcct tttagtccat attcttggat gaggtagtcc
     7441 cgcgagaaac tgtcgagtct tgcataatac aaaacttgtt ctacgtattc atcgattttt
     7501 gataattcat tttctagtaa tgtatacttt tgatcgtcaa tatcgaactc gatcgaacga
     7561 actagcaatt tagaagcgga aagtggcact ttgatttcat ggacccatga gtcaatataa
     7621 tctttttggt cttgttgatt actgattgct gattgcatta cttcttggtg ttcacggatc
     7681 agctgattga tatattcttc taccagtttt tcttcttctg tcctcccacc actcaaataa
     7741 ttttgcaaag gggaagtttt ttgtatgtcg gctaattttc cccaccatct tctcttgctc
     7801 aagtaaccac ccagcagata aaaaatcaga aagacgccct caatcaaagc taaataagca
     7861 attgtgctca aatcaacttt gatgtttggc gccagccaca tcacaaagat cgtcaatgca
     7921 ataaaaaaca gccatcccaa gatcaatgtc cactgatctt tcaaatattt aaaaaaattc
     7981 atcttaaccg ctcatccttt cagggaacga tatatccctg tcctactttt gtttcgatat
     8041 atccttggat acctgcctgt tcgatttttc ggcgtaaccg attgatgttg actgtcaatg
     8101 tattgtcatc tacaaatcgt tcgtcatccc acagtgctcg cagcaatttt tcccgactta
     8161 agatctttcc attgtgcttc atcaaaataa ataatagacg atattcattt ttgctcaaat
     8221 caatgatctc tccgttgatt tccatacttc cgttgtcaac attcaacgta atgccattgt
     8281 ggctcatcat ttcactagat aattctgtgt agttgtaaga acggcgaagt aatgcattga
     8341 tttttgcaat cagtacatcg atttggaaat gttttgtcac aaaatcatct gcccccatgt
     8401 tcatagccat gatcatatcc atattcgtat tcctgctgga gataaaaatg atcggtactt
     8461 tcgatacttc acgtattttt tgacaccaat aatagccatc caaaacggga agattaatat
     8521 ctagtaatac aagctgtggt tcttctcttt caaagtctga gaacacttga ttgaaatcgg
     8581 tcgttccaaa ggtttcaaat tgccatttct caagttcctc catgatcaat tgtcgaatga
     8641 tctcttcgtc ttcgactacc atgatttttg ccataaaaaa tccctccatc tttcttcttg
     8701 ctactacaat atcaaagaag cccttgtgaa acaagttggc ttacctggaa aatgtcattt
     8761 tagcaaacgt taaaaaaaac tggaaatatg cgatttaatc gcgtttgtct cttccaagca
     8821 gcttaaattt attatgttat tcttaataat tggtctatta tctgttattt ttctttcgtt
     8881 gtttttgtct tttaaatttt cgataacacc aataaccgaa aagcgttagc actagcaata
     8941 tacctagaag gagtataagt aatcgataaa gatggtattt ttttgtctgt tctatctttg
     9001 aagagacttc ttctgtaaag ggaactcgtc ttcctgtcac tagtaatcgg tgagtgttca
     9061 cggtgtaagg tgtacaagtg ataagtgtga ctagatcctg cttttctcta attgctaatg
     9121 tatttgtttt tgttggaagg acaacaattt tctcaacgac ttcataagcg agcatccgac
     9181 catacacttc aagatagaat cggtcatctt gctccatttc ttcaagatcc gtaaagagtt
     9241 ttttctcagc aataccgcta tgggcggtaa tcacggaatg tgtactctct cctccagttg
     9301 gaaaagaggt gccttgcagg actgtcgctc ctctatctaa caaaagatca ttcgtttcat
     9361 caaataaagg caagctaaca ttgattttcg gaatatagat ggctccgatc atatgttgtt
     9421 cgtaatattc ttttcctgga ttttggacat ttctaaccgc ttgctcaaaa ggatctttga
     9481 cttgtcccat tcctgggatt actgatgtat gagcaagcgt ctgatttttc ttttcatgca
     9541 ctgctaattg ttgctcatgt ttttcttctt tatcgcgttc aagctgcttt tgataattct
     9601 cgatccttct ttgatccaaa taattgctga gagcatctgc taaaaatgga tatgtaaaaa
     9661 ctgcagctcc ggcaagaaac atcactgcca taaataattg taataaaaga ctgatttggg
     9721 tttgaggtct attacctatt ttctttttca ttttcttcac cttcttattt aaaaaaactg
     9781 cagcaacctt tataaaaccg attgctgcag cttatttttg ttgcttatac ttcagacttc
     9841 attttagatt tacggtacca gctgtacgct ccaatcatca aacttattcc gattagtaag
     9901 aatgccaaga gcccgttccc accagtagca ggaagtgacc cgcgtttcat attcggtact
     9961 ctttctggcc ctggattttc tactcctacg atctgaattt cttcattata gcttccataa
    10021 gagatgataa attcagtaaa ggctgcctca cctgttggaa gtacataacc ttctggcgcc
    10081 atggtctctt tcatttgata ggttccataa tctaaccctt gaacctcaaa gtatccattt
    10141 ccatctgcga caaattctgt tgcttgtccg cttgttgccc actcaattct atctgctcct
    10201 gattcataaa cagctaaagg cgactcccct ttataaaata ttgcgtattc agaacggtct
    10261 tcattccaca attcgaatct tgcatcttta agcaattcac tgcttgatgc atcatatttt
    10321 tcaaactttc ttccacctgt tgtgactttc tctggcggct ctactccttc ttcataatca
    10381 aattcatcat ttcgtccgat ttcaataacc gctgtgttgt tatggaaatc atccgcttct
    10441 agttctgtgg aaaccttcaa attatacgtg atcgttaaat ctcctccacg tgcatctgct
    10501 aatctaccta aagtatctac tttggctact ccattttctt ctgtcaaaat cgtcacgaca
    10561 aacccgttgc ttactacttc tactatataa tcttgtcctt ttgttaaaat cgtctcgccg
    10621 gctcgcactt ctatttcctg atcataataa gttagaccaa ctgtaggcgt atctgtgatt
    10681 ttgtaatttt tgataacagc gcttccgttt tcaaggacag aatcgatgaa ataaggaata
    10741 ggtgctgtga tcgtgtaaga agccgttttt cctctttcta gatcaatata agaaatcgtt
    10801 tcttgttctc cagcttcatt aataatcgtt acttgtctaa ggtcgctttc ctctacattc
    10861 atctgtttat ctgcttctct gatttcgttt ttcggataca aatgcacatc tgtattgtat
    10921 gtgtctttcc atgtagaacc atcccacacg ccatccacca tatcaggcat catcactggc
    10981 atcataacaa ccattggaga cgcgattctg gaaatatgag ctggcgaata agtttctaag
    11041 aaaagataga ctttatctct agaagaaggt gcttcttccg tcacttggac ggtagagaat
    11101 accgcttctc cagcttcatt cgttgtttgt ttgtcaatac ttgtcagtcg atattttaaa
    11161 aaccaacttt ctgtgtgccg tttttgaatc cattcaatcg cttcggcaga attaagaccg
    11221 tcttctcgtt ccggtgttaa aggattatct cgaatcaaat cgtaatatac ttccgttacg
    11281 tcaaacaaat caaaatcgac tcctggaata ttttcttcac caaaatccgt agaaaccaat
    11341 ccgctattcg gtcgttcttc cggaacagat gagaaacctt ttttgtgtaa tgtaatcgtc
    11401 acttcacttg gtttttgtgc gacttcttcg gctttgacta gttttccgtt cccgaaaatc
    11461 cctattcccg cagtaacgag taacagtaca ctcattcccg tgacaatcat ttttttattc
    11521 atttttttct ccccgttctt tttttat
//
LOCUS       DAZUKZ010000080        11543 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000080 DAZUKZ010000000
VERSION     DAZUKZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.082"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(238..723)
                     /locus_tag="ACG8EF_002040"
     CDS             complement(238..723)
                     /locus_tag="ACG8EF_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFS4947335.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    813..1158
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1203..1844
                     /locus_tag="ACG8EF_002041"
     CDS             1203..1844
                     /locus_tag="ACG8EF_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4947336.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1863..2498
                     /locus_tag="ACG8EF_002042"
     CDS             1863..2498
                     /locus_tag="ACG8EF_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4947337.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2516..3331
                     /locus_tag="ACG8EF_002043"
     CDS             2516..3331
                     /locus_tag="ACG8EF_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4947338.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3650..5086
                     /locus_tag="ACG8EF_002044"
     CDS             3650..5086
                     /locus_tag="ACG8EF_002044"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4947339.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5275..7722
                     /locus_tag="ACG8EF_002045"
     CDS             5275..7722
                     /locus_tag="ACG8EF_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS4947340.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7952..9019
                     /gene="rlmN"
                     /locus_tag="ACG8EF_002046"
     CDS             7952..9019
                     /gene="rlmN"
                     /locus_tag="ACG8EF_002046"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFS4947341.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            9009..9215
                     /locus_tag="ACG8EF_002047"
     CDS             9009..9215
                     /locus_tag="ACG8EF_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947342.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9212..10315)
                     /locus_tag="ACG8EF_002048"
     CDS             complement(9212..10315)
                     /locus_tag="ACG8EF_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4947343.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10868..11416
                     /gene="aac(6')"
                     /locus_tag="ACG8EF_002049"
     CDS             10868..11416
                     /gene="aac(6')"
                     /locus_tag="ACG8EF_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS4947344.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3838 a         1950 c         2485 g         3270 t
ORIGIN      
        1 aatcaaaaga ataaaattcc gatttataat cgaaaaaaga agcattttta tatatgcttc
       61 ttttttcgta gaatcaataa atatggattt ggctaactat ggaagaatac tcgtattagt
      121 tttcagcgtt catactagtt gttacattgt ttgattggcc aaaagaattt ctgatatatt
      181 ttgtgctaaa taaagaaaaa gacgcttgaa ttgggaaata agaaaactac tctatcatta
      241 caaattttca taaactggca gatgttctaa gtaaacaagt atcttttcaa agtcggaaaa
      301 atccataatc aacgtagtgg tattgatatt tggatgaaag cctacggtat cttgacgatc
      361 aatagcagca tcgatcacga cttggatttt tttctctttg tcgtgcatca atgctaatgg
      421 agtcactgta cctgctggaa cgcctaggag gtcaggaagc tgttcttcag aaacaaaaga
      481 taagcgcttt tcttttaatt gttcagctag ttgttttaaa tcggcgtgct tttcatcagg
      541 caagatgaca agatagattt gttttccttt tcttgatttc aataatagat tttttacttg
      601 cggaccaggt agggaaaaag ggacattttt gactgacgta atcgcaggat gatcgacttt
      661 ttgaaaagag atacccagtt tttttagtag ttcataagct tcttgttcgg ttgcacgact
      721 catcgcgcat caccctcctt tttttattag tgtatcataa aaaagagttg acaaatgaaa
      781 aataatcatg taaattacac gtaaagttaa aacaaatgac atcgaaaaga agagtagccg
      841 ttaggtgttt ttagagagtc tctggttggt ggaaagaggc agcagttaac cgtgaaacac
      901 atcttggagt cgattttctg aagcgtatag taggaaaatc cggggagccc gttacagctg
      961 cggtcgttgc tagatgacca gataaggctg gcttttccgt gaggagtcgg taaaaaaagg
     1021 tggaaccacg tgattaacgt cctttgatct tcggatcaaa ggactttttg ttttcatctg
     1081 aacggcaacg actgtgtgag gttttttctg caaggaaaaa ataaacagag gtggaaccac
     1141 gtataatacg tcctcttggc atttgccaag aggatttttt tattttaaag gaggaaaaga
     1201 agatggagta tttgattgaa gttatgccgc aattattagt aggagcactc acaacattga
     1261 aattattttt atttaccttg attggctcat taccattagg gattgtttta gcattaggat
     1321 tgtctttgca ttttaagccg ctacgctact tgcttcaaac atatgtatgg gttatgcggg
     1381 gaacaccgct gttgttgcaa ctgattttta ttttttatgg gttgccgacg attgggatcg
     1441 tttttgatcg tttcgaagca gcactcattg cctttatctt aaactatgca gcttattttg
     1501 cagagatctt tcgtggaggg atacaatcga ttcctcaagg tcaatatgaa gcagcgcaag
     1561 ttttgcaatt aagtcccttt caaacggtga cgaaaatcgt catcccacaa gtgacaaaga
     1621 ttgttttacc ttctgtaggg aatgaagtta tcaacttggt caaagattct tccttgatct
     1681 atatattagg tttaggtgac gtaatgcgtg ctgggaaaat cgcgatggaa cgcgacgcga
     1741 ctttactgcc tttgctaggc gtggcaatca tctatcttac attgactggt attctaacaa
     1801 tcataatgaa acaagttgaa ggaaaattaa attattaccg ctaactgata ggaggaagaa
     1861 atatgattga attgaagaaa gtgaacaaag catttggtga aaaaaaaata atcaaagatt
     1921 tgaatctaat cattccagat gggaaaacat tagctatcgt aggtccttcc ggtggcggta
     1981 aaaccacgct tttacgtatc ttggctggct tagaaagttc tgacagcgga gaattttatt
     2041 tagacggtgt accgttcaac ccattcgagc ttaaaatgaa agaacaagtt gtaggagtcg
     2101 tattccaaga cttccagcta tttccacatc tgaatatttt tgaaaacatt accttagcac
     2161 caaaaatggt attgaaacag acaaaagaag aatacaccaa aaaagcagaa gaattagcgg
     2221 cttcattagg gattagtgat ttattgaata tctatcctta tcagctttct ggcggacaaa
     2281 aacagcgggt agcgattgcc agagcattgg ctatgaatcc acgggtattg gcttatgatg
     2341 aaccaacaag tgctttagat cctgaattac gccagcaagt cgagcgtttg atcctatcat
     2401 tgaaagaaga tggggtaacc caaattattg tcacccatga tttagtattt gcagaaaatg
     2461 tcggtgatca attactaaaa gtttccccaa ttcattaaga aaggagaaga tagagatgaa
     2521 aaaaaccagt tttctattcg ctttgatcgc aggtcttctt ctttttgcag gctgttcgaa
     2581 caaaaaaact tcagcagata attgggcgac tatcaacgaa gagaaaaaaa tcgttatagg
     2641 tttagacgat acatttgttc ctatgggatt tagagataaa gacgggaagc tcacaggatt
     2701 tgatattgat ttagccaaag cagtctttga acaatatggg attacggtag actttcagcc
     2761 cattgactgg tcaatgaagg aatttgaact gaacaatgga acgatcgatt tgatatggaa
     2821 cggctattcc aaaacgccgg caagagaaaa gaaagtgcaa tttacaaaac cttatatgga
     2881 aaacgatcaa gtgctgatca cacctaaaga atcaggaatc acaaactttg gacaaatgaa
     2941 taataagaga ttaggtgcgc aaaatgggtc atctggttat gatgtattta cgaaacaacc
     3001 agaagtatta aaagagatag tcaaaaacca agatgctgtt ttgtatgata gttttaatga
     3061 agcattgatc gatctgaaaa gcagacgggt cgatggtctg ctgatggata aagtatacgc
     3121 agactattat ttgaaacaaa gaaacgaact agaagacttc aacataacaa aaggttctta
     3181 tgagagtgag gattttgcag ttggtgcacg caagtcagat agtacattag tgaaaaaaat
     3241 caatgaagcc ctagatcaac tagagaaaac aggtgaattc caaaaaatat ccgataaatg
     3301 gtttggcgaa gatgttacgc cgagaaatta acaaataggg gactgtgaca aaagacgtgt
     3361 cacagtccct ttttccgaat aaacagtgtt ctcctgccaa ctccttgtga ctcggagctg
     3421 aatggcaggc tcacaacctc ttggtaaacg gtgttcaaaa gccagttttt cggtattaag
     3481 ccaaatttat gcaaaaatgt agaaagccat tttcacaaaa tttttcttac tactcgaaac
     3541 taagcgactt tttcacaacc tcctttacat aattttattt tgttgtggaa ggggtttcgt
     3601 gttattctaa aagaggaacg attgaaggtt ataggaaagg aagagatgaa tggaacacaa
     3661 agaacttacc tcatttccaa aagattttct gtggggctct gcttctgcag cgtatcaagt
     3721 agaaggagct tggcaagaag atgggaaagg agaatcagtc tgggatcgat ttgtacggat
     3781 acctggaaag acatttaaag gaacgaacgg tgatttggca gtagaccact atcatcgtta
     3841 taaagaagat attgctttaa tgaaagaaca aggtctgaaa gcttaccgct tctctgtagc
     3901 ttggacacgg atttttccta atggacgagg agaagtcaat caagctgggc tcgcttttta
     3961 tgagagattg atcgacgaat tgattgaaaa cgatattgaa cctatgttga ctttatatca
     4021 ttgggatctt ccacaagcat tgcaagatga atataatggt tgggaatcac gtcagatcat
     4081 tgaagatttc actcattatg cagaaacatt atttgaggca ttccgcggga aagtccatta
     4141 ctggattagt ttgaatgaac aaaatatctt cacttcatta ggttatttgt tagcagcaca
     4201 tccgccaggt gtgacagacc cgaaacgaat gtatgaagtt aaccatattg ctaatttagc
     4261 aaatgcatct gtcatcaata aatttcacga gttagaaatg cctggaaaaa tcggaccaag
     4321 ctttgcgtat acgccaaatt atccaattga cagtaatcct gaaaatgttc tggctgctga
     4381 aaatgcagaa gacctgatgg ccaattattg gatggatgtc tatatgtggg gaaaatatcc
     4441 gattgctgcc atgaggttct tagaagaaaa aggatgggca ccaacgattg aagctggcga
     4501 tgcagaattg ctagaatctg ctaaaccaga ttttcttggg atcaattact atcaaacggc
     4561 gacgaatgca ttcaatccat taaacggagt tggtgctgga aaaatgaata cgacaggaaa
     4621 aaaaggttca tcagaagaaa ctggagttcc tggaatgtat aaaaaagttg agaatccatt
     4681 tgtcgaacga acaaattggg actgggagat tgacccagaa ggattaagga tcggactgcg
     4741 tcgtatcaca agccgttacc gcatgccggt actgattacg gaaaatggct taggagagta
     4801 tgacaagttg acagaagata aacaaatcca tgacgactat cgaatcaatt acttacaaag
     4861 ccacgtcaaa gccatcaaag aagcaatttc ggatggagca gaagtcctag gctattgcac
     4921 ctggtcttat acagatttgc ttagctggct gaacggttat caaaagcgat atggatttgt
     4981 atatgttgat caagacgaaa cacaagaagg atctttggaa cgatataaga aagacagtta
     5041 ttactggtac caaaaactga ttgaagaaaa tggagagaac ctttagttca catacttaaa
     5101 acaagaaggt acggctggat atggccgtgc cttttcttga taaaatcatt tagaaattaa
     5161 gaaaaactgt aaaataagct gaaaaatgaa agaaagcgtc agttctttca aacaaaactt
     5221 tagttccctt actttttttt gtataatgaa acttgatgaa atgagggata ataaatggca
     5281 caaaagaaac ggcctactaa aaaaaggaaa aagacgaaaa aaacgaaaaa gcaaaaacag
     5341 cagcaggaac aaatgctgac gattggtgtt gggtttgcct tgattttatt tgctgtcttc
     5401 ggctttttaa aattaggttt tttaggaata ttgattgcaa acggctttcg gatcattgca
     5461 ggaaatactt accaaatcct ctgtctttta ttagccgtat taggattttg gattgtcatt
     5521 aagaatacag agttttcaat tggtaaaaac cgacgctggt ttggcggcat actcttttat
     5581 ttcggtatct tattattgtt acatgcccat ttatttggca aactccatac tggggaacca
     5641 aatatcatgg ggacgacatg ggatttgtta gccagtgata taaaacaaag tcaagtagac
     5701 aataacgttg gtggtgggat gattggagca atcctttatc attttactta cttcttgatt
     5761 gcacagcctg gaagttattt agcagcagtg ctattactgg caggaggagc attcctgttc
     5821 agtaatctgg aaggctatca gctgttaaat ggtatccaat caattggaga acgagtacag
     5881 gaattattgg aaggtgatcc tgccaagcaa gctcgaaagc aagcggcaaa ggaagagaga
     5941 atgaaacagc gcgcagaagc gaaagaagca aggcgcttag ctgcaaaaga agctgcagaa
     6001 aaagaagctg cagaatatga gaagaaaaaa gcggtgagcc agcaaaggga aaatactgct
     6061 gatgaatggc aggaaccaga aaatacagag ccggagcagc taagttttgt tccaatcgac
     6121 agttttcaag aaaatatcca tcctgcgaat ctagagaaac cagtaccaga tacaccaaaa
     6181 cagaccaata cagcagaagg tttttcagat gagttgccag aggatgacgg tacctcatta
     6241 gaatttgaga ttgaagcaga acaggaaaat caagactatg aattaccgac agttgatttg
     6301 cttgattcta taccaactgt ggatcaaagt gatgagtata aaaaaattga aaaaaatatc
     6361 ggcgtattag aacaaacttt ccaaagtttc ggtgtcgatg ccaaggtcgt caaagccagc
     6421 ttaggaccat ccgtcacaaa attcgaagtc cagccggctg taggggtaaa agtaagtaaa
     6481 attgtgaact taacagatga tatcgcgctt gctttagcgg cgaaagatgt ccggatggaa
     6541 gcaccgattc caggtaaatc actgattggt attgaagtac caaatggaaa gatcagtatg
     6601 gtttccttta gagaaatcat cgaagcacaa ccaaaccacc cagataaatt actagaagta
     6661 cctttaggac gtgatgtttc tggacgggta caaacggctg atctttcgaa gatgcctcac
     6721 ttactcgtgg ctggttcgac tggtagtggg aagtcagtag caattaatgg gattatcaca
     6781 agtatattaa tgcgtgctaa gccccatgaa gtaaaactga tgatgatcga tccgaaaatg
     6841 gttgaattga atatgtacaa cggcatacct catctgttga cgccggtcgt gacgaaccct
     6901 agaaaagcag cgcaagcctt acaaaaagta gtacaagaaa tggaagaacg atatgaaaaa
     6961 ttcgcggcaa ccggagtaag aaatatttct ggttacaatg aatttgttca acagaaaaac
     7021 ttggaaaacg gaacgaaaca tccaacctta ccattcatcg tagtgatcgt agatgaatta
     7081 gctgatttga tgatggtagc cagcaatgaa gttgaagatg cgattattcg tttagcgcaa
     7141 atggctcggg cagcggggat tcatatgatc ctagctactc aacgtccaag tgtggatgtt
     7201 atcacaggaa tcatcaaagc aaatgtacct tccagaatgg catttgcggt atcaagcggt
     7261 acagattctc ggacgatcat tgattctaat ggggcagaaa agctcttagg gcgaggagat
     7321 atgctattct tgccaatggg tgagaataaa ccgatccgtg tgcaaggagc attcatttct
     7381 gaccacgaag tagaacgggt cgttcaattt gttacagatc agcaagaagc tcattatgaa
     7441 gagaaaatga tgccgacaga tgaagttgaa acgacaggtg ctccagaaca gccacaagat
     7501 gagctgtttg aagaagcaaa agcacttgtc gttgaaatgc aaacggcaag tatttccctt
     7561 ctccaaagaa gatttcggat cggttacaac cgagcagcac gtttagtaga tgaactggaa
     7621 gctcatggag ttgttggccc gtcagaagga agcaagcctc ggaaagtatt cattgagcag
     7681 gaagaaacat cagaaacgtt gccggacgat aatgacatgt aaaacttgtg tgatgaaggg
     7741 ttgaaaaacg aataaccaat aaaaagacaa ggcatgatga taccaaaaga aaaaatggtt
     7801 tttttccatc ttttctaata cggtatgatg tcttgtcttt ttagtttttg tgagattaaa
     7861 gttctattcg atttatttgt gaagtattgc attaaggaaa tgaatcatgt atagtatgta
     7921 gcgaaacttt gagaaaaaga gggaaaatac tttggaaaaa cagtccattt atggcttgac
     7981 gaatgaagaa ttaatcaatt ggtttataga aaacggcgag aaaaaattcc gagctgccca
     8041 agtttgggaa tggttgtatc aaaaacgtgt aagtaatttc acagaaatga cgaatctatc
     8101 gaaacaattg atcgaaaaat tatcggagca ttttatcatc aatcctctaa aacagatggt
     8161 cgtccaagaa gcttcggatg gtacagtaaa atatttgttt gaactccctg ataaaaacat
     8221 gattgagact gtactgatga gacaagaata tggcttgtca gtttgtgtga cgacacaagt
     8281 aggatgtaat atcggctgta cgttttgtgc aagcgggctg ctcaaaaaga accgtgatct
     8341 gactgctgga gaaattgttg cacagatcat gatggtccaa cattattttg atgagcgcaa
     8401 tcttggcgaa cgcgtttcac atgtcgttgt aatggggatc ggtgagccat ttgataatta
     8461 cgacaatgta atggactttt tgcatatcat caatgatgcc aaaggattgg caattggtgc
     8521 tcggcacatt acagtttcca ctagtggatt agcacacaaa atcaaggaat tcgctaataa
     8581 cggcttgcaa gtcaatctgg ctatttcctt gcacgcgccg aataatgagg tgcgaacatc
     8641 gatcatgcgg atcaaccgaa gtttcccaat tgagaaattg atggaagctg tggatgagta
     8701 tttagagaaa acaaaccgtc gaatcacgtt tgaatatatc atgttgaatc aagtaaatga
     8761 tagaccagag catgcgcaac aattagcaga tctattaaaa gataagaaaa aattagcata
     8821 tgtcaacttg attccatata atccagtcag tgaacatgat caatattctc gcagtccaaa
     8881 agctgatgta ttgagatttt atgatgtatt gaagaaaaac ggcgtaaact gtgtgatacg
     8941 taaagaacat ggtacagata ttgatgcagc ttgcggacaa ttgcgaagca agcaaatgaa
     9001 aaaaacagtt gccaaataat cgtatcctac tcgttttttc aattaccata aaaggagcag
     9061 ttttcttaat taaggaactg ttcaacttaa ataagagaaa agattctata gcgaaagcta
     9121 taaagctttt tttgacaaga aaaaccctca tcaagcaaag aaatgtcctc aatttgatga
     9181 cagcctgctt gatgggggtt ttagcaatta tttaactgat tttcttatat ccttgctggc
     9241 tttcttgtgt agcatgataa gtttctacgt caagaatggc ctgtttaaat ttttctcggg
     9301 tgatttcata aggcacatgt ttaatatcat ttgttgccat ggctttatct aagattgctt
     9361 ctaaatcggt cgtgacattg agattcatgt cggttaacga tactggaagt tggttctttt
     9421 tcataaacac atagatttta tcaaacaaat cgaactgctg atccatcatc aataaggtca
     9481 gtacaccata actgacagat gctccgtgaa ggtatccctc cattttttct agaacagtcg
     9541 taccgtaata gaatgagtgt gccatagcag aattatagtt attgttaact aaaacagaaa
     9601 ccaatccggt agtaacaatg atatccgaaa cgacttgttc aattgcatat gagggacgat
     9661 cgtcggcact atctttcaat gcttgcgtac cataagtcaa gagacgatca atgcacattt
     9721 ttccgatttg gattcctaat ctatccgtgt agcctagatc atcgccacga gcagaaaaag
     9781 tagattcgac ttctttggat aaagcatcgc caatacctgc ccataagtat ttatcaggtg
     9841 cttcggcaat aattgctgta tcaatgaagg tgtgtacagg tggttttgaa ggaaagaaga
     9901 gtccgtccat tacgccttct tcgttataga tgacacatac cgcagtgacg ggcgaacagt
     9961 tcgaaccaat agtgggaaag gtgaagactg gtttagctag tttttcacca atgactttta
    10021 cagtatcgca agcacggcca ccaccgactg caaacaggac gtctgctttt tggacacttt
    10081 catgctggat caactgttca atatttccat aagttgaatt acccccgtac cagagactgg
    10141 cgacaacctc tatttcctgt gtcaaagcta cttttatttt gttttctgcc tttgaaagtg
    10201 cagtcttccc tccaatcagc actacttttg tcccatattc tttgacgatt ccatttgctt
    10261 tttcatacgc atctgttcca atggtataat ttggtaagaa aacactttga ctcatattca
    10321 tttccttctt tcttcgttga aacgggttaa aattttaaag agtgatcgat ctgactgcgg
    10381 ctgtagatgg agtagaggtg aacctaagac aggataagtg atgaataagg tggcatgaac
    10441 accttgaaat catcaataag tatcctaaaa acgaacaagt acaaaaacat tgaaaacgac
    10501 aataatgaat ttttgttcct cttagtattt ctaccgattc cctttgcttt tttgtaatta
    10561 tctaaaaatt ctaaaaatta agattattat atgaaaacag gttttatttg tcaattcata
    10621 attggttttt tttaaagcaa aagataatct atatcatgat gaacaaacag agttttttcc
    10681 tccaattgat ggctttcctg atctttccta ttttgaataa agtgtttctg tataattttt
    10741 ttattttata agtaagataa ataatgaaag aatagagata ctgattggta gctaaagatt
    10801 tttgagcgag aacatttcgt tttttcaaaa ggactgttag aataagcaag aacaaggggg
    10861 aagacgtatg ataatcagtg aatttgaccg taataatcca gtattgaaag atcagctttc
    10921 tgatttactg agactgactt ggccggaaga atatggagac agctcggcag aagaagtaga
    10981 agaaatgatg aatccagaac gaatcgcggt agcagcggta gaccaagatg agttagtagg
    11041 atttattggt gcaatccctc aatacggtat cacaggttgg gaattgcatc cattagttgt
    11101 agaaagctcc cgacgaaaga accaaatagg tactcgatta gtcaattact tagaaaaaga
    11161 agtagcttcc agaggaggaa tcacgattta tttaggtacg gatgatttag accatggaac
    11221 aacgttaagt caaacggacc tgtatgagca tacatttgat aaagtggctt ccatccagaa
    11281 ccttcgtgaa catccgtatg aattctatga aaaattaggt tataaaatcg taggtgtctt
    11341 accaaatgca aatggctggg acaaaccgga tatttggatg gcaaaaacga ttattcctcg
    11401 accaaattct caataataaa aagaggttgt gacagaagta atcagcttca agaaataaga
    11461 aggaatttac gaaaattact tcttgaattt ttgtgaaatt ccaacttatt tccgaagaag
    11521 ctacttttgg aacaccgtgt acc
//
LOCUS       DAZUKZ010000081        11408 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000081 DAZUKZ010000000
VERSION     DAZUKZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.083"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..444
                     /gene="rpsN"
                     /locus_tag="ACG8EF_002050"
     CDS             175..444
                     /gene="rpsN"
                     /locus_tag="ACG8EF_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS4947345.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            459..638
                     /gene="rpmF"
                     /locus_tag="ACG8EF_002051"
     CDS             459..638
                     /gene="rpmF"
                     /locus_tag="ACG8EF_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS4947346.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            670..1047
                     /locus_tag="ACG8EF_002052"
     CDS             670..1047
                     /locus_tag="ACG8EF_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947347.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1068..1217
                     /gene="rpmG"
                     /locus_tag="ACG8EF_002053"
     CDS             1068..1217
                     /gene="rpmG"
                     /locus_tag="ACG8EF_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4947348.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1241..2224
                     /locus_tag="ACG8EF_002054"
     CDS             1241..2224
                     /locus_tag="ACG8EF_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS4947349.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2254..2376
                     /locus_tag="ACG8EF_002055"
     CDS             2254..2376
                     /locus_tag="ACG8EF_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS4947350.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2394..3917
                     /locus_tag="ACG8EF_002056"
     CDS             2394..3917
                     /locus_tag="ACG8EF_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS4947351.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            3941..4180
                     /locus_tag="ACG8EF_002057"
     CDS             3941..4180
                     /locus_tag="ACG8EF_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4947352.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4373..4834)
                     /locus_tag="ACG8EF_002058"
     CDS             complement(4373..4834)
                     /locus_tag="ACG8EF_002058"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4947353.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5029..5247)
                     /locus_tag="ACG8EF_002059"
     CDS             complement(5029..5247)
                     /locus_tag="ACG8EF_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4947354.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5365..6981
                     /locus_tag="ACG8EF_002060"
     CDS             5365..6981
                     /locus_tag="ACG8EF_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS4947355.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7222..8562)
                     /locus_tag="ACG8EF_002061"
                     /pseudo
     CDS             complement(7222..8562)
                     /locus_tag="ACG8EF_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9000..9146)
                     /locus_tag="ACG8EF_002062"
     CDS             complement(9000..9146)
                     /locus_tag="ACG8EF_002062"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947356.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9185..9379)
                     /locus_tag="ACG8EF_002063"
     CDS             complement(9185..9379)
                     /locus_tag="ACG8EF_002063"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4947357.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9289..9621)
                     /locus_tag="ACG8EF_002064"
     CDS             complement(9289..9621)
                     /locus_tag="ACG8EF_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4947358.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10135..10326)
                     /locus_tag="ACG8EF_002065"
     CDS             complement(10135..10326)
                     /locus_tag="ACG8EF_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4947359.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10420..10806)
                     /locus_tag="ACG8EF_002066"
     CDS             complement(10420..10806)
                     /locus_tag="ACG8EF_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4947360.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3793 a         1795 c         2193 g         3627 t
ORIGIN      
        1 gattatctct atgtaccgat aaattcgttt tcaaatagtt atctattcat atgagtttta
       61 tttcatgtta tttattgttg acaagagcaa ctcttgttgg taaagtatca gtgtcaaaac
      121 gtaacgatta cgatttgagg tgtctaaatc aaaataaaag gagtataaag gtatatggca
      181 aaaaagtcga aaattgctaa agcaaagaaa caaatggcga tgattgaaaa gtatgcagat
      241 aaacgtcagg aattaaaagc cgcaggagat cgcaccgctc tcgctaaatt gccaagagat
      301 tcaaatccga atcgtctgag gcttcgtgat cagacggatg gtcgtccaag agggtatatg
      361 cgaaagttcg gtatgtcccg tattaagttt agagaacttg cccatcaagg gttgattcct
      421 ggagtaaaaa aagcaagttg gtaaaaggag gaaatatcat ggcagtacca gcaagaaaaa
      481 catctaaggc gaagaaacgg ttaagaagaa ctcatcaaac tgtagtaaaa ccagaaattt
      541 catttgacga agcgaatggt gattatagac gtagccatca tgtatcttta aaagggtact
      601 ataatggaaa gaaagtaatt aaaggagctt caaaatagtt taatatataa cttttaggag
      661 ggaatcaaaa tggaaattat ttacccacct ttagttgaag aaggattaaa gtattatctt
      721 gaaacgaccc agcaatcgct agataaatgc actttttatc gttcgatggt agagagagga
      781 attattactg aaacgggttt accgacccaa caggcaattg aaaatggatt agtcaaagat
      841 tactatgagg accaaggctt atcatttgat gattttttaa gaatctatcc aatttttgaa
      901 gagtatgacg aggagttatt tcaatgtatc gatggttatt gggagatacc tgttgatatg
      961 aaagaaaatt tagtttcaca attggaatct ggcgagttaa cttttgaaga tgcacaacaa
     1021 attcaagcat atcttgaaga tcgataagta ttgaaggagg aagatttatg cgtcaaaata
     1081 ttattttgga atgtgtcgaa actggagaac gtctgtatct caccagcaaa aataaacgga
     1141 ataaccctga acggttagaa ttaaaaaagt attcgccaaa attgcgcaga cgggcaattt
     1201 ttaaagaagt gaaatagcta agtggtaaaa aggtgacaaa atgggcatac cagttacaat
     1261 cgtttctggg tttttagggg ctggaaaaac aacgctaatc aatcaagcct tgcaagtctc
     1321 accctacccc agggaagaaa ttattataat tgaaaatgag tttggtgaag ttgggattga
     1381 tcacaaatta ttagttcata gcgaggaacg ggtgttacaa ctaaataatg gatgtatgtg
     1441 ttgcagtctt cgcagtgatt tattagcaat attgatttct ttactggaaa caatagaaga
     1501 acatcaacaa ccaatttcac aaatcatcat tgaaactaca ggtatagcag atccccaacc
     1561 aatcattcaa acacttttaa cagcgcctca actaagagga agagtatata tcgatagttt
     1621 attgactgtt attgatagtg acagttttga acttattgaa agagaaccac aagcccttaa
     1681 gcaacttgtc atggcagatc gcatttttgt gtctagaaaa caaaacttga atttatctgc
     1741 actaaaaaaa ttacagagag aaatcaaaag tattaaccct ttatccgata ttcgaatgtt
     1801 cacacatagt gaaacgattc aaaaaaagga tttttataat ttgaaaaaat ttaatcatcc
     1861 cttagttttt gaaagtgatt catcagaaaa tcactataat ggcaaatact atcatcaaca
     1921 ccatcaagta catgagttta aatcaattct cttaacttca gatcaagaca taaaaaaaga
     1981 ttttttactc caatggattg actggctgat tttaaccaac caagggagcc tgtatcgttt
     2041 taaaggattg attgctttac aagagcagga ttttatagtt gccatgcaag aggtaaacga
     2101 acaggttaat tttcaataca ccactcgaca aaaagaccaa accactatta ttctaatcgg
     2161 aaaaatgttg gatgaagaaa ggatcaaaca gagttttgat aaattactgg cggaaagcaa
     2221 ataatgataa gtagtgattg ggggtaattt ctaatggaga aaacggatat atctagtgca
     2281 tatcgacgac ttaaaagccc gaatattaaa actagaaaaa gggcattaaa aattatcaaa
     2341 gaacataaac gaaataaaat gaagaaatta gcataattaa ggaaggaaaa agaatgaaga
     2401 aaatcagtgt aggacttatt gggatagcag ctctaggatt attgggagct tgctccagca
     2461 ctaatgatgc aaaagtatcg aatgataaag atggaaaatt agaaattgta acaacattct
     2521 atccaatgta tgatttcacc aaaaacatcg tcggcgatga agcgaacgtg gatttaatgg
     2581 ttccagcggg atcagaacct catgactatg agccctcagc taaggatatg gcaaaagctc
     2641 atgatgctga tgtttttgta tatcataacg aaaacatgga atcatgggta ccaaaagcta
     2701 aagaaagttg gaagaaagct gggcctaatg tagtagaagg aacaaaagac atgattttat
     2761 tgccaggtag tgaagaagaa gatcatgatc acggagaaga agatcaccat catgaactag
     2821 atcctcatac gtgggtttca cccaaaatgg caattaagga agtttcaaat atcaaggatc
     2881 agttagttaa attgtatccg aaaaaagcaa aagtatttga gacaaatgct gaaaagtatt
     2941 tgactaaact gaaacggttg gatgctgact ataccactag tcttaaagaa gcgaaacaaa
     3001 aaagttttgt gacgcaacac gcagcctttg gatatttagc tttggattat ggattgaaac
     3061 aagttcctat tgctggacta agtcctgaag aagagccatc ttctggtcga ttagctgaat
     3121 taaaagagta tgttaaaaaa aataagatca attatattta ctttgaaaag aatgctaatg
     3181 ataaaattgc aaaaactttg gccaacgaag ctgggataaa attggaagta ctgaatcctc
     3241 ttgaaagtct tacaaaagaa caaatggata atggtgaaga ttatgtttca gtaatggaag
     3301 acaatctaaa agcgttagaa aaaacaacta tggttgctgg tgaagaggta gttccagaaa
     3361 aagaagcgaa ggatgaaaag acagttgcta atgggtactt taaagatgcc gatgtgaagg
     3421 atcgcgaatt gagtgattat acaggcgaat ggcaatctgt ttatccgttg ttaaaagatg
     3481 gaattttgga tgaagttttc gattacaaag caaaaatcaa taaagacatg acagctgccg
     3541 agtacaaaga ttattatact actggctata agacagatat tgatactatc aacattaaag
     3601 acaacaccat tgattttgta gtcaacggtg aacatcatca atacacatac aaatatgttg
     3661 gatacaaaat tttgaattat gaaaaaggaa accgtggtgt tcgttttaat tttgaaactg
     3721 atgatgcagg tgctggtcgt tttaaatata ttcaatttag tgatcatgga attgcaccaa
     3781 gtaaggcaga gcatttccat attttctttg gtggagaaag tcaagaaaaa ctctataatg
     3841 aattgcataa ttggcctact ttctatccag cttcattgtc taaacatgaa attgctcaag
     3901 aaatgatggc tcattaaaat tcaatttttg gaggaaatac atggtaagaa aagtattatt
     3961 gtctatggat tccaagaata ttgatgctta tcggcgttat cgatttgcaa agcaaatgag
     4021 attaaatggg agtacattag ctgatattgc tgcaacttta catgttagta ttgacacagt
     4081 tcatagagta ttaaaacggg atcctgattg ttgttttatc aaggtcaata ctggaagaag
     4141 tagacgttta gcaaaacggc ataatagctt ggccaattaa ttgataatac cagagtagat
     4201 catttttccc ttatttggat ttgatctgct ttttattatg tcttgaattg tcaaatcaag
     4261 cgcaacactt tatcaaagaa gacttcataa ggtgttctcc aatctaaaca ctttctaggt
     4321 ctgttattca gtttttcaat catctgattt atcgttgagt tatctaccgt agctatctct
     4381 ttcgattttg gcaagtattc tcgaatgagt ctattcgtat tttaatttgt tcattgttgc
     4441 caagggcatg aggatctgga aaataaaagg gcatattatt caatgcttct gtaactgcga
     4501 aatgattgga gaattctttt cctctatctg gagtgatcgt tttgaccgcc tcatttggaa
     4561 tactgcttaa aagttcaatc attttctcgc tcacataagc ggctttcttt ctggggacct
     4621 tctttcctgc taaaaggaag catgattttc tgtctgtcaa agtcactaaa caagtaccac
     4681 cagttttgcc agctacaata tcggctttcc aatgaccaaa atcacttcta tcatctgcgg
     4741 attttggacg gccatggatc gaatgagtaa ggcaaatctt tccacgagac tcgacatgat
     4801 tttttgtatg tcttgttttt cctcgatggt gcaatgaacg gatcaggcct ttattgccgt
     4861 gtgagagggg ttctgtatcg aaaagatcat tgcaaattgc acgttagatc gtgataaaac
     4921 taattgaaaa gtccatatgt tcatgcttca aacgattcga gatttcctct ggcgaccact
     4981 gaaaatcgac aaataaatga cgaacaattg accaaactca cggatcattt agaatgtgat
     5041 aacgtccgca tttttttctt cgataagcat aatttttttc cgctttggaa ggtgaatagt
     5101 ttttttcttg tttcttctca gttcatgtaa aatagtaaac gggctatgct gaatgtcgtt
     5161 gccaattata ctaatagaaa atccttcttc gtgaagcaga aagattcttt ctcgttcgag
     5221 tatagtaaga tgacgatact ggctcataat tattttcctt ctaggagtgt tctcgacaaa
     5281 cttatactag ttggatatcg attatgactc atttttatgt gttgcactta aagtgtaaat
     5341 tcaaggtata gaaagaggtt ccctatgaaa aaaataccta ttacaatctt aactggctat
     5401 cttggttcag gaaaaacgat acttctaaat catattctgt acggtgatca cggaaaggaa
     5461 attgctgtca ttgtaaatga cataggggaa gtaaatgttg atggggtatt agtcgacaag
     5521 aaaggtttta aaagaactgg agaaaaactt gtttcgatgc cgaatggatg catttgttgt
     5581 acattacgcg aagatttgat tgttgaacta gatcaattag tgcaattttc tgacttgaat
     5641 caaattgtta ttgaagcttc aggaatcagc aaaccaattc caattattca agcaattata
     5701 ttagctaaaa accctttaga cattgatttg acccaacgat tagagattgg tgccattatt
     5761 atagtagtgg atgtaaaacg tttttgggat aatttcgaaa cgggtgaaaa gattcaggaa
     5821 agaaaagctg aaggtaatgt agttgaagaa gaacaggata ttcgtggctt gttaattgat
     5881 caattcgagt gttgtaacat attactttta aataatagtg acttagtgtc aaccaagacg
     5941 atttacaaaa ttaacggtct aattgctaag ttacagtcca atgccaaaat aattaggaca
     6001 attaaaggtg aagtagactt atcgaaaatt ctaaatacaa atctttttaa tatagatgag
     6061 acttcctatt cagtgggctg gttcaaagag ttagagcttg gctatcaaaa tcatattcct
     6121 gaaacagaag aatatggtat ctcaagtttt atctatcgaa atcggttact ccttgacgca
     6181 caaaaatttg cccatcttac ggataaaaat tttcctggca atattacaag agcaaaaggg
     6241 tttctttggt ttgcccaaca tagtgatttt tcgacgatct ttgaaacatc gggtagctat
     6301 aaaagtattg aatcattaaa ttattgggta gctagtttac ctaaaattga gtgtcaaatg
     6361 attatacaaa aaaatcccga attgaaggaa gcctggcatc cgttgtttgg tgaacgcatt
     6421 aataaactag tttttattgg tatgaagatg gatcaacaat gtattaagga acaacttgag
     6481 ggttgtttaa ctgattctct taatgcagat aatttccgaa aaatttcacc atttccatgg
     6541 aaaaagtcta atatgtttag ccagatatcg ttaatgaaat ttttaagaaa agctggtgtc
     6601 aagataggcg atgtcataat tattggcatt ttgattatta cctcttttat tcccttattt
     6661 gtattctcta tagaaaagga gaatgaaaca actgaagatg ctatcaaata tgccatcgtt
     6721 cgaattgatg gcaaggaaat ggatcgcttc gatttggata agatcaatca taaactcgtg
     6781 aattatcatc ctgcgaaggg acaatataat attgttgaga ttaaagatgg aagaattcga
     6841 gttaaagaag ataatagtcc tgatcaaatc gcggttagaa ctggatggat ctcaaaaccc
     6901 ggtcaaacga gtatttgttt accccataag ttagttatta gtattgagaa aaaagaaagc
     6961 acagattact acatttacta atctggaaat agatgaggag gtttttaata agaaaaagat
     7021 attattatta ataatagcag gggctttatt aagtgtgtta agcgcttgtg gacaagaaaa
     7081 agaagcggtg acaacaggca caagtatctt taacgggacc tgcaatggtt tagttaacag
     7141 tcggttagat tacttatttt ggctcttcgt caatgagtgt tggtgaagaa attcaataga
     7201 ataatcgaga tactcacacg gctagacagc tatcttgtct agccgtgttg ttgttctggc
     7261 tttagtcagt tgggtagtat tcttagtttt cttaaaatat tttcctcgat ataaaaagat
     7321 tcgttctcgg tagttttgaa agtttctata gccataggca attcgtttta gtactttgat
     7381 atggttattc aaacattcta tagggccgtt ggaataggga acgcgcaacg ctcgtttgat
     7441 ttctgtttgg tatttcttga aggtcgtgaa cactggttgg tagtgctctg gcaactcctt
     7501 gtagtcttct tttaggagcg cgacgaattc aggaacatct tgtcttctga taacggaaag
     7561 aaaatcctgg tagacttcgt agcctcgctt taaggaatca tcataagata atagccgatc
     7621 gactatctct gcttctgtca gatgatctcg aaatgacgga cgccagtagc gatgctgacc
     7681 tttcaattcc cacgcattct tttgaagtag tttccagtat ttctttaagt gcttcgaccg
     7741 acgatcccct tttttaaagg tcttcattac ttgaattcgg tggttggtaa aagcccgacc
     7801 aatgtgttgg ctaatatgaa agcgatcggc gacgattcgg gcattcggaa aacactcttt
     7861 caccaatgtg cgatagggtg tatagaaatc tgaaaccact agacgcactt gttcacggac
     7921 ttttataggg tagcgcaaga aatagtcgcg taaaggattg agttgccgat tttccacaat
     7981 atcaatcagt tgatgggtct tgccgtccat catgacaaag ctcatggctc ccgagacatt
     8041 tttcactgat ttgaattcat cgaaacacac cacttctggt aacagctggc tcaaagaaat
     8101 ggttttaggc cgaaggctgc gaagtattcg aatgacagtg gtcggagaaa tgtgcttcat
     8161 acgtgcgatc aaagacatag aaagaggttc gcttaatagc tcaagaatgg cttgtttgac
     8221 tctgcgagca atggagcagc gacgatcact aactgaatct tctgctaaaa aagtctttcc
     8281 acagtgttta catttgaagc gctgtttgtt aatccgcaga atggtcttgt agttactgac
     8341 atcattcaat aagatggata cttttttcca gccctattta atgatcgttt gttcattttg
     8401 atagtggcga tgaaagcatt cctccggttg ataggtcaat gtgccatcaa aaacaaaaca
     8461 tgtttcgtca ttgattcgct ctttgcgaaa ggcatcttca tgaaagataa gatttaggtc
     8521 tagaatatct agtaactctt tggtaaactc tgaataggac atcttgaaac actcctttga
     8581 atggttttcg tcgactttat tctaaaggat tttaaggtgt ctgtttgcgt ttattagaaa
     8641 aaagaaaaag tattggcaac agaaggcgtc accaacacta gatattatag atcccttatt
     8701 ttcttcgctt caaattgtca aggagtcatc ataaattttg tatggattct tttgtttggg
     8761 tagtagtctt ctaaataatc ttggattaac accttcatct ctattttact tttatatttg
     8821 ttggagtcga ttaattctca tttgattgat cgatgaaacg cttcaattac tgcattgtca
     8881 acaggagtcc cagtttggct cactgaatgt gtgatgtggt ggtcgttcaa cagctcttga
     8941 taaataaaag accgatgctg gctacctcta tcggaatgaa ttaccgtagg caattctcct
     9001 taataaattt gtaatggacg gacgacaatc tcttttttca tgtgtgaatc taccatatag
     9061 taacgtagaa tacgagttgc taagtcaata taggtggaca aatagaggcg accgtcatta
     9121 catggaatgt aagtaatatc catcacccaa acagcatcaa tttggttctg ttcaaattat
     9181 cgattcaaca tttttttacc atttctttga ttgttttttc tctaccatga gaagatttgc
     9241 gtcgatgaaa tcctcttgtg cataaattca tttcacgcat aagaacagct acaacacgtt
     9301 tattgatttc gattccttca tcgtaaagtt tttgagtgat ccctggagat ccgtatcgat
     9361 ctttgtgttc gtagaacact cgtcttcctc ttttagtcgt ttattttcct gttcaagaat
     9421 gtagaaatct gctttatcag gtaatctttt tccttgtcca gcaaaagcat ttttcccgta
     9481 tcgacgatat tctgaaaccc atcgggaaat tgttggacga gagatattgt attcttcagc
     9541 aattttcttg gtagttactt ttttctcaag gatatcctgt gaaatcttta gtttaaattt
     9601 acgatcatat gtttttctca atagagttca ttccttccgg taacgtaact gatagtcaag
     9661 aaaagtgtat catagcccaa cttagtatca gggtttgtca actaaactgt ggaagttgat
     9721 agataagagt ttttaacaac caaaattctc ttgactattt ttgaaatcag acaaattttg
     9781 attggacaca tagttgaata aatctaccgt cgttacggaa ggtaaatcgc atgcttttca
     9841 attctagagg agaagtcctc gttgaagtac catagcccag ttctggattc gacctcctgc
     9901 ccacttcata cgtaaataaa gtaattttag gagtgagact gtgacaaaag ttatttttaa
     9961 gctaaaaacg aattaaaaaa cgttgataat taaaatcatt cgttttagat tctatgaatc
    10021 attatagaac gctgtaaaat cgatacttta taattataaa gaagatagtt tcaaatttat
    10081 tgttcgaaat ttgaaactat ctttttactt atgtcacaga ctcccttagt tgagttattc
    10141 tttaatttcg aatttcgaga tagctagata tattgcaagc atacattctt gataacaatc
    10201 ttgatcatat tttccagttc tccaggattg cttatgaatc aaagagctaa atcttttgag
    10261 aattgcctca acagcttctt catttccagc ctttgctaaa atgaaatctt cttttaaaga
    10321 attcataaat ctaccttctt cccatggact tataatctat ttctttggga aggaggagaa
    10381 cgagcaaaga agcatacaaa attagttatt atgcagatat taaaccagca ttttttgaat
    10441 ttttttgtaa agtcgatgtt ttaaatttgt cgcaccctgt cttgtaatcc ccagaacctt
    10501 tccaatttct ttatcagttt tatcaaagat aaatttttcg ataagaaaaa tttgttcagg
    10561 ttctttcaac aaaggaagga tttttgctaa ttttatatct tcaacaagga gagtcacatt
    10621 taaaacatta atcggttgtt taaattttat agtatcttta gtaaacaaat atggtgggag
    10681 ttgatcaagt tgttcatgtt tttgaatttc ttcttgtttt ttataataag tattagcggt
    10741 gttcaaaatg accttttcaa agaatcgaat caactgggca tttacgcgtt cgtcgttatc
    10801 tgacatattg cacacctcct ccattaagat ttaaagggaa aagaggcaaa aaacacgaaa
    10861 agaagctaat gcaattatgc tgcgctgcga ataacttcta taagctcaca tgcattgata
    10921 ggactgctga tgtttatttt caaaaatgtt tttttagttg ataatggata gttatcttta
    10981 tacttagaaa agaaaattca tatatcttct taaatatcaa acatgtcctt atggctaaga
    11041 aaatgtcttg agctttttac gtataataaa tcttttctaa aacctccttt tggataatat
    11101 tcttattcgt gtatagattg tattttttat ttcttccccc ttcataatta acaactagaa
    11161 aagagttcga atagacacgt atttgaacga aaatttttat tggattccca aattggaata
    11221 tttagaaacg aaaaattcct attatctgta tagtgcttag ataataacgg atcataataa
    11281 ttattgtctt tgaactagat atatgtaaat agtattcatc atgatgaaaa atgatgagtt
    11341 ctcggtgata aaatgtactt tttacggtgg aatatacatg aaagcgataa gtggatattt
    11401 tataatga
//
LOCUS       DAZUKZ010000082        11286 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000082 DAZUKZ010000000
VERSION     DAZUKZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.084"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(302..2311)
                     /gene="metG"
                     /locus_tag="ACG8EF_002067"
     CDS             complement(302..2311)
                     /gene="metG"
                     /locus_tag="ACG8EF_002067"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFS4947361.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2579..2857
                     /locus_tag="ACG8EF_002068"
     CDS             2579..2857
                     /locus_tag="ACG8EF_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947362.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2954..3775
                     /locus_tag="ACG8EF_002069"
     CDS             2954..3775
                     /locus_tag="ACG8EF_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS4947363.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(4060..5070)
                     /locus_tag="ACG8EF_002070"
     CDS             complement(4060..5070)
                     /locus_tag="ACG8EF_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS4947364.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(5082..6035)
                     /locus_tag="ACG8EF_002071"
     CDS             complement(5082..6035)
                     /locus_tag="ACG8EF_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4947365.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(6025..7095)
                     /locus_tag="ACG8EF_002072"
     CDS             complement(6025..7095)
                     /locus_tag="ACG8EF_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4947366.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(7101..8144)
                     /locus_tag="ACG8EF_002073"
     CDS             complement(7101..8144)
                     /locus_tag="ACG8EF_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4947367.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(8145..9086)
                     /gene="opp3b"
                     /locus_tag="ACG8EF_002074"
     CDS             complement(8145..9086)
                     /gene="opp3b"
                     /locus_tag="ACG8EF_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS4947368.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            9170..9517
                     /locus_tag="ACG8EF_002075"
     CDS             9170..9517
                     /locus_tag="ACG8EF_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFS4947369.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9587..11248)
                     /locus_tag="ACG8EF_002076"
     CDS             complement(9587..11248)
                     /locus_tag="ACG8EF_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4947370.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3174 a         2302 c         2058 g         3752 t
ORIGIN      
        1 tgcattcaaa ctttatgaat gtaaggacaa taaaaaagag taccagatga atcaattaaa
       61 atctgcccga ccctttatca ttatgctttt tttctgtttt tacgattcct attagtatta
      121 ttctaaatta acaaaaatca gcgaagctcg tacttctttt tctaggattt attttggata
      181 gttctctgtt tctgtattat atgattggct tcaagtaata agaaggaatt tacgaaaatt
      241 gcttctcaaa tttcggaaaa agggggtatg acacaactag tgtcataccc cctttgaatt
      301 tctatgcgat ttctgagcca tttggcatgg attttggtgc ttcgattact tgtaaagagc
      361 catctggtgc ttctgcagaa aggatcattc cttgactgat ttgtccacgc atctttcgtg
      421 gtttcagatt tgctacaatg acgacttttt tcccgatcaa ttcgcttgga tcaggataaa
      481 attcagcgat tcctgacaag atttgacggt cttggctgtc tccagcatct agtcggaatt
      541 gcagcaactt atctgcccct tctacttttt gacagttgat gacttctgct acttttagtt
      601 ctactttttc aaatacatca aatttgattt gtttttcttt cgttgaaaca agttctgttt
      661 cttctggatc ccatttgact gtatcttcgt ttgtttgagt accttcagac attttttctt
      721 gaataaaagc aacttcttct tccatatcta aacgtgggaa gattggtgta ccttttgcaa
      781 ctacttttgt tcctgaagga aattcgccaa agtgtaatcc ttcaagattc attgtttcag
      841 agtccaatcc tagttgattg aagatcttct ctggcgtttc tgtcatgatt ggctgaagta
      901 atatcgctgc gatacgcaaa gattcagcta aatggatcat gacactattc aattcagctt
      961 tcttttcttc gtcttttgct aatacccatg gttctgtttc atcaatgtat ttgtttgcac
     1021 gagaaatcag cacccagatt tcagccaatg ctgtattgaa ttccattttt tccattgcat
     1081 catggtaacg tccgacaacg ttagcagccg tcgtagacag ttcactatca aatggagtga
     1141 caagtgaggc atagtcagga acgagtccat cacaatattt gttgatcata gcaatcgttc
     1201 gattcaataa gttccctaga tcatttgcca gatcataatt gacacgggaa acaaaatctt
     1261 ctggcgtaaa gacaccatct gacccaaacg gaacagcacg aagcaaataa taacggagtg
     1321 catctaaacc ataacgttcc acaagcattt caggataaac gacatttcct tttgatttag
     1381 acatttttcc gtctttcatc aatagccagc catgcccaaa tactttttta ggtagaggca
     1441 aatcaagggc catcagcatg attggccaat agatcgtatg aaagcgaacg atttctttac
     1501 cgaccatctg aacatctgca ggccagtatt tttcgaatag actttcatct tcggagccat
     1561 atcctagtgc agtaatataa ttggataatg catcgatcca tacatacacc acatgtttcg
     1621 gatcactctt gactggtact ccccaagtaa aagttgttcg tgaaactgca agatcctcta
     1681 atcctggttt gataaagttg ttgatcattt cgtttttacg agattctggc tggatgaatt
     1741 cgggatgttc ttcatagtat tccaatagac ggtctgcgta tttgctcatg cggaagaagt
     1801 atgattcttc ttttaccaat tctacttcgt gtccacttgg cgctttacca ccgatcacgt
     1861 taccttcgtc atctttgtac acttctgcaa gttgtgtttc tgtaaagaat tcttcgtcag
     1921 aaacagagta ccagccttca tattcaccta agtaaatatc tccctgttct aataaacgat
     1981 caaatatttt ttgaacagct gctttatggt aatcatctgt cgtgcgaatg aatttatcat
     2041 aactgatatc aagcgttttc cataatttct tgacatcagc cgccatttta tctacatatt
     2101 cttgcggttt tacacctagt tcttctgctt ttgtttcgat tttttgacca tgttcatcca
     2161 ctccagtgag ataaaacaca tcaaagccca tcaaccgttt ataacgagca attgcgtcac
     2221 atgcaatcgt agtatacgag ttgccgatat gaagtttgcc gcttggataa tagattggcg
     2281 tagtgatata aaaagtttcc ttttctgcca cggggctctt cctcctttat cgcatgatgc
     2341 gaattcgttt ccttgctagt ataccataaa aaaagcatgc tgttagcttt ttttcaaaat
     2401 gaaaaagact ggataaacga cttctatcta cgttcaatct atcctttaaa aggatgaata
     2461 ggttgaataa atgcgggttt ttgttttgtt tcatatctac taaaaaacgg ctttttattt
     2521 ctcccctcac acgtggtata ataaagacta tctatcttag taagggactg gaacaatcat
     2581 gcagaagaaa catctttttt ttacacttag tattgctttt ctttcattag cacatttaat
     2641 attcagctat ttttatattc gtatgtatgg ttattttaac ctccatgggc atttgaacag
     2701 ttttatgaca gcagcttgga ttttgcgttt tatcattgac gtatatatcg ttatttgtgg
     2761 cttctttgcc attcgtgaag agcggtataa agtattgccg ttttatctgc ttttcttttt
     2821 attcaatctc atacttccat ttatttttca tatttaaatg ataacctaga tttttcaagt
     2881 aaatccttgg ttttttttca gagtctgcta caataaggta aaaagtactt ttactgtcaa
     2941 tggaggtggt cagatgaacc gttttgcttc taaagaagaa gaaaaattat attatgaaca
     3001 taaagcaagt gaagaagaat ttcttaactg gtatcaaaaa caggaacgtc cagaatatga
     3061 caagccctct ttgactgtcg atatcgtact gatgtgctat aacaaagaag aagatcagct
     3121 gaaagttttg ttgatccaaa gaaaagggca tccttatcga aattcatggg cattgcctgg
     3181 tggctttgtc caaaaggatg agtcaactgg agaaagcgtc ttaagagaaa caaaggaaga
     3241 aactggtgtg gttatttcaa aagaaaatat tgagcaactg catacattca gtacgcctaa
     3301 tcgagatccg cgaggctggg tcgtcacagt cagctactta gcctttattg gagaagaacc
     3361 gctaatcgcc ggagatgatg ccaaggaagt acgctggttc acactagaac gtcatagtaa
     3421 caagatccat ttatctagcg gagaagtggc tatctcgctt gatttagtca ctggagagtc
     3481 ttctggcaaa gatactttgg cttttgacca tagtcaaatc atcttgaaag cattcaaccg
     3541 tgtagtcaat aaaatggaac acgagccgca agttctacaa gttttaggta aagatttcac
     3601 gattacggaa gctcgaaaag tatttgctaa attcttaggg atcgattata aaacgatcga
     3661 tcattccaat tttaagaaag ctatgctcca atatttcgat gagatcggtg aacgtcctgt
     3721 aggaatcggc cgtccttcaa aaatctatca gctcaagccg gggcaccatg aataaggttg
     3781 tgagcaaaag attcagacct aatcattcaa gaaaaaacgc aaaaacaact ctttgtgagt
     3841 tacgtttttt gtttcagaat tttgatgtat tcgtaaactt tctgaataaa aaattaacaa
     3901 tagcagaaaa agctcgtacg aactttttct gctattgttt tattggtttg tcataattag
     3961 tgagcttttc ttcaaaagaa aaattcctag aaacaaaagt cattaagact ttttattaac
     4021 aaggctaacc ctaacgctcc tatcagtttc attcattttt tatcccttga taaccgaatg
     4081 tctgcccatt ttttctacta cttggctctt tcttacggaa ttgctttctt ctgctccgtc
     4141 ggtattgatc aaaagaaatt ttttattata ccattgggac ttataacgca attttgttat
     4201 taatttatgc cagtcgtgaa tagaaaaatc agcatgtgcc tcttcttttg gaaaagattc
     4261 tacaaataag tagtcatatt gtttgaccaa tttcccagat aattgttcaa gataatcttc
     4321 tcgctggttc ttttgtttca gataaagatc cattactttt ctcttttgtt tctgataatt
     4381 gcttgcctga tctaacctga tctttctatg gtgagcggag cggactttca cattcaattt
     4441 ccgctgtgca tgttgcagct tttcttctgt ctggtgaaat tttggtaaag ttacttctag
     4501 aggtcgagat gtttggatca atgtcttcgg atgataggcg atcccgatcc atttagctaa
     4561 agattctttt tttactggcg aaacatcaca aagtagtgcg acataaaatt cttcattata
     4621 tcgagcagaa atcgtagcgg agcgaatcga tccttcaact ggccgatgct gatttatctt
     4681 gattagttct ttttgttttg gtaactttaa gtagccattt tccagataga tcgtacgggt
     4741 ttggttgttg gtcgtataag attgccacat actttttttc gttttaagct ttgggaagct
     4801 ggcacgtccc ttgaaataat tgcgaaaagc ccgatccaaa ttaagctgtg catttgctaa
     4861 agcaagactg tctacttctt ttaagaatgg gtattctttt tttaatgtag cgggtgtgag
     4921 cgaaaaattg gctactcctg tttgttgcca acttttctgc cgctttttca gcaaatggtt
     4981 ataggtaaat cgagcacatc caaaagtacg aatcaattgc tgtttttgtt cttctgtcgg
     5041 atacatacgg aatttaaaag cttttagcat ctatgtccct cctagttagc aaccagaaga
     5101 gattcctttt gtcgttttag tttttttgcg taatatggga cttcctgctc agttgcatag
     5161 acaaaatgtt ctggtgcgat ttcacgaagt tgacgtactt gtccgtcatc tggttcaggt
     5221 tgatatttga ttcttctgcg ttttctctca tggtctggat ctggtagagg aatagcagat
     5281 aacaaacttt ctgtataagg atgaacaccg tgattataga tttcatcact tgttcccatc
     5341 tctaataaca agccgttatg cattacaccg attcggtcac taatatgttt aaccatcgac
     5401 aaatcatgtg caataaatag ataggtcaat tgatgttctt tttggagatc ttgcaacaaa
     5461 ttgaccactt gggcttggat cgacacatct agtgcagaaa ttggttcatc acaaatgata
     5521 aaacgcggtt ttactgccaa tgctcgtgca ataccaatac gctggcgttg cccacctgaa
     5581 aattcatgag gataacgagt accgtggctt ggattcaatc caacagttct caatagatca
     5641 tcgacttttt ttgcacgctc ttcagatgat ttagctagtc cattaacatc aattccttcg
     5701 gcaatgatat ctctgacttt catacgagga ttcaacgaag cgtatggatc ttgaaagatc
     5761 atctgaacat catgccggaa tttcgtcaaa ccttttttat ctttgatctt cgttatatct
     5821 tgaccgtcga ataatatctc accatcagta ggttcattca agcgaatgat cgtacggcca
     5881 gtagtggatt ttccgcttcc agattctcca actaaaccga atgtttctcc ttcataaatg
     5941 tgaaagctga tgtcatttac agcatgtact tcattctttt ttcctacatt gaaatactgt
     6001 ttcaatcctt taacttcaag tagtttattt ggcatcagca attcctcctt gaccgtaagt
     6061 atgtttttgt ttctctttaa agatagccca gcgacgttgg atttcttctg gcggtgtcac
     6121 tttgggtgcc tgcggagcaa gtagccaagt tgctgccttg tgtgtttttg atagttcaaa
     6181 aaatggtggt tcttgttctg catcgatttt catcgcgaat tcatttcttg gatagaatgc
     6241 gtctcctttt ggcgggttca ataaatccgg tggagaacca ggaatagcat acaatcgatc
     6301 gttcgcacta tctaatgtcg gcattgaacc aagcagtccc caagtatagg gatgttgcgg
     6361 gttgtagaaa atttcttccg atgttccaac ttcaacgatt cttcctccat acatgacagc
     6421 gacacgatca gcgacattcg ctactacacc aaggtcatga gtgataaaga taattgatgt
     6481 atcgattttt gtctgaattt ctttcagcag ctccaaaatc tgtgcttgaa tagtcacatc
     6541 aagcgctgtt gtaggttcat cagcaatcag gatttccgga taacatacca gtgcaatcgc
     6601 gatcacgatc cgttgacgct gacctcctga gaactgatgc ggataactgt tgatacgttt
     6661 ctcagcttcg ggtattccaa ctaatttaag caattctaat gcagcttttt gtccctcttt
     6721 ttttgaaact ttattatgct ttctaaggga ctcagcgact tgcttcccaa tgggcatcgt
     6781 cggatctaaa gaggtcattg gatcttggaa aatcatcgct atttctttac cacggatttt
     6841 ttgcatctct ctttctgatt ttccaacgat atcgttacct ttaaagagga tttgaccagc
     6901 atctatattg gcgttgcttg acagcaagcg catgatgctt ctggtcgtca ctgattttcc
     6961 acttccagat tccccaacga tcgccaatgt ttctccttta ttcaaatgaa aattgacacc
     7021 gcgaatcgct cgtacctttc cagcatacgt atcaaaagag atttctaagt ctttcacttc
     7081 taaaattttc gtcatcgtct tcactctttc attttaggat caaaggcatc acgcaatcca
     7141 tcagcaagta agttaaagcc aatcatgata accgataaag ttactgccgg aatccataat
     7201 agataaggca gatacaggaa tgtcttgtac ccttcgctca acatcgttcc taatgaagct
     7261 gacggaggcg taagacctaa accgataaag cttaaaaagg cttcgaagaa aatagcagat
     7321 ggaatactaa acatcatttg aataataatg acactagaga tattcggcaa aatatgtttg
     7381 aatgcaattt tcattcttga ttcacctaat gtcaatccag ctaaaacata ttcttgatct
     7441 tttaatttca ttgtttgtgc acgaacgata cgtgccattg gtatccagtt tgtgatggcc
     7501 atagcagcca cgattgaaaa aattcctggt tcaaaaacga ccaacatcaa aatcatgaca
     7561 acgaggtttg gaattcctga taagatttcc aaaaaacgtt gcatcacgtt atcgacacgt
     7621 ccgcctaaca aaccagaaac caacccgtaa gctacaccaa tcgttacatc tagtaatgca
     7681 gcaataaatg cgatcagcaa cgagattctc attcccataa ataagcggct taatacatca
     7741 cgtcccaaac cgtctgttcc taaaaagaaa tttacgtttg aagggacgtt tgcttgagca
     7801 tatttatcaa caagttcacc accaaccatt gcttttccat tcaatccatc gatattgatt
     7861 cccggaatac gaggaggcaa attgatataa gccacgtttt gggcagttgg atcatgagga
     7921 gaaacaaaga ttgtgatcac agagatcaaa atgatgacca atagtaaaac cattgaaata
     7981 acggcagctt tgtttttctt taaacggcgc catgaatctt ggataaaatt caaagaaggt
     8041 gcagtaatct gttcacgttc ttcttcggta tttttttgta atggaagaaa ttcatccgca
     8101 gggatatctg caactgtttc ataacgcttt ggtatcattt ccatttatcc tcggctccct
     8161 tctgaaacac gaattcttgg atcaactaga ccgtacaata gatccacgat caaaataaca
     8221 aaaatcagca taaatgagta aaggatcgtt acagccatga tcgtaggata atcatttgtt
     8281 gtgattgact taacgaattg ttccccgata ccaggaatag caaagatatt ttctaccacc
     8341 agtgatccgg tcatcaatgc tacagctaac ggtcctaata aagtcatcaa tggaatcaaa
     8401 ctatttcgaa gaccatgttt aaaggcgatt tcccaacggc tcaatccttt tgcttttgct
     8461 aattccacat aatcactatg cagtacttcc accatttccg tacggataaa ccgagcagag
     8521 tcagcaagcg gcgacatggc caacgcaatt gttggtaaga ttgtgtacac aaagccatcc
     8581 cattttgcga ttggcaaaac ttgtaatttg attgcaaaga tatattgtaa taaaacagca
     8641 aatacaaagt ttggaatgga acgacctaat atcgctacga gtgtagaaga tgtatctgcc
     8701 catgtatttt gcttcatggc agagatcgtt cctaaaatcg tccctaggac tgttccaaag
     8761 ataattgcct gtaaccctaa ctgcaaggat ggtccgattc ttccagctaa caaatgtgcg
     8821 actggctggt ttttaaattg gaaggaaatt ccaaaatctc cttgaagcaa attagaaaga
     8881 taaatcccat attgtacgat aactggttta tccaaaccga cttgtttatt tagcatctcg
     8941 attgtttcag gactcaaacg ttcctgattc gtatatggcg tgcccggcag caattgcatc
     9001 aagaaaaagg tgattgttgc aattaaccaa agcgtgatga tcatgaaaaa gacacgtttt
     9061 aatacatatt tgatataact attcactaat gtttccccct agcttttgac ttctgacaga
     9121 tgtttcgcta cattattaga taatgaacca aaaataaagg agcaatcaaa tgaaaaaaaa
     9181 atctatcccc tacgcagtag cttttctact gattctggtt attttaataa aaaatctcat
     9241 caaccattcc tttacactca ttcaattatc taatgatcta ttcttatggt cattgccgtt
     9301 tctgattatc ggcggttttt tatgggtctt ttcttcgggt ttcttcgatc attttcagcg
     9361 atcggtccat cttgcacgaa caaggaaccg aaagaaaaaa ccagaatttt cctcgctctc
     9421 gtctgccagc tacggtatgt acagcttttg gctgatcatt gccggaatac taattgctct
     9481 ttctgctatt ttcatgcttt tttctttgtt aggataagtg aatatgagaa cgaggttgcg
     9541 acatttgccg cagcctcgtt ttttctcatg ctcacggtat taacgattat tcttcaacgt
     9601 atgtccattt gtaatcccat gaagcgcctg ccgcatggtg aacgatacct ttgatttttt
     9661 cattcactag gtgaccttct gctttttggt acaccggaac gacacccata tcatcagaga
     9721 tgatcttgat tgctttttgg aaatcatccc aacgggcttg ttcatcgcca gcgtctgttg
     9781 atgaagctgc tttaactgct tcgtcatatt ctgcattgct ccagcgacca cggttgtatg
     9841 aattacctgt gacaaacaaa tcagtaaagc tgcttgggtc tgcatagtca gcagcccatc
     9901 cgcctagtac tgcgtcaaaa tcacccgaag ttgagcggtc aagacgtaca gagaatggaa
     9961 caggtgttgg tttgactgtt acgccatcca agttttcttg gattgagttt tgaacatatt
    10021 cgattacttt tttcgatgaa tcatcatcag aagccatcaa atcaaattct aaagaatcaa
    10081 cgcccaattc ttttttcgct ttttcccaat attcttttgc tttatctttg tcataagaaa
    10141 ctaattttcc agcttctttt gcaaagtctt cgttcgttgt tggacttttt accatatctg
    10201 atgggatcaa tcctgtagag acaactgatc cgtcacctag tacttgtttt actaaagctt
    10261 cacgatcgat agaataagaa atagcttttc ttaggttcac attgttgaat ggtgaattct
    10321 tttcacgttg attgaattcg atatagcttg tccgcgcttc tttaacagaa gtataagctg
    10381 gatcgtttgc gttttgctgt gctaactcac cagacaagat cacgtcatct gcttggccat
    10441 ctttgaataa gttcaatgca gtagaagatt cttttactac actgactttg atttcagaaa
    10501 gttttactgt atctttgtcc cagtaatgat catttttctt ataagtccat tctgtatctg
    10561 ttcctggtcc atcaaaatct tctaatgtga atggtccatt ataaacagct ttatcacttg
    10621 cagatgcata ggcatcccca ttttcttcca ctacggattg tttttgtggg aaaaatgatg
    10681 ggaaagcaag cagatattgg aaatatggtg tttgtttcgt caatttgatt tctaattcat
    10741 agtcaccaac tgctttgatc cctagttctg atttatcttt ctttccagct gtaatgtctt
    10801 ctgcattttc aactggcgca tacaggtaag catattcaga agctgtagct gggtctactg
    10861 ttcgttgcca gccataaacg taatccgctg ctgtaacagg atcaccattt gaccattttg
    10921 cgtcttctcg taatttgatt ttgtaagtta atccgtcttc actgacttct gccaactcgc
    10981 tagctcctgc cggctgtggt ttgctgtctt catcaagacg gtaaattcct tcataaacgt
    11041 tattcaatgc tgtaaaactg attgtatctg ttgcaactga taagtcagct gtaggcattt
    11101 cttgaggcac gactaagtta aatacgcctc ctccatctgc tgaaccgctt tttgctgaac
    11161 tactgcttgt ggctgaactg ccgccataac atccagcaag tacaaggccg cataccgcaa
    11221 ccaaaccaaa tgtaaatttc tttttcattt tacgtacccc tttattggaa ttttcagaat
    11281 aattaa
//
LOCUS       DAZUKZ010000083        11219 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000083 DAZUKZ010000000
VERSION     DAZUKZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.085"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            181..1440
                     /gene="lysA"
                     /locus_tag="ACG8EF_002077"
     CDS             181..1440
                     /gene="lysA"
                     /locus_tag="ACG8EF_002077"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFS4947371.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1427..2227
                     /gene="dapF"
                     /locus_tag="ACG8EF_002078"
     CDS             1427..2227
                     /gene="dapF"
                     /locus_tag="ACG8EF_002078"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFS4947372.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2230..3111
                     /gene="dapA"
                     /locus_tag="ACG8EF_002079"
     CDS             2230..3111
                     /gene="dapA"
                     /locus_tag="ACG8EF_002079"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFS4947373.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3108..3824
                     /gene="dapB"
                     /locus_tag="ACG8EF_002080"
     CDS             3108..3824
                     /gene="dapB"
                     /locus_tag="ACG8EF_002080"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFS4947374.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3830..4525
                     /gene="dapD"
                     /locus_tag="ACG8EF_002081"
     CDS             3830..4525
                     /gene="dapD"
                     /locus_tag="ACG8EF_002081"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFS4947375.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4541..5542
                     /locus_tag="ACG8EF_002082"
     CDS             4541..5542
                     /locus_tag="ACG8EF_002082"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS4947376.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5539..6900
                     /locus_tag="ACG8EF_002083"
     CDS             5539..6900
                     /locus_tag="ACG8EF_002083"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFS4947377.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6860..8011
                     /locus_tag="ACG8EF_002084"
     CDS             6860..8011
                     /locus_tag="ACG8EF_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4947378.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7986..9155
                     /locus_tag="ACG8EF_002085"
     CDS             7986..9155
                     /locus_tag="ACG8EF_002085"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4947379.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9330..10709
                     /locus_tag="ACG8EF_002086"
     CDS             9330..10709
                     /locus_tag="ACG8EF_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4947380.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10990..11219
                     /locus_tag="ACG8EF_002087"
                     /pseudo
     CDS             10990..11219
                     /locus_tag="ACG8EF_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3733 a         1956 c         2503 g         3027 t
ORIGIN      
        1 cttctaatag ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat
       61 atttcgaaat atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca
      121 aatctttatc aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa
      181 atgacagaat tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca
      241 cctctgtatg tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc
      301 tttgtatcga aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt
      361 gagatgctgc atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa
      421 atctataccg ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac
      481 aagacgccag aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat
      541 aatttgatgg aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt
      601 atcatgcttc ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg
      661 catattgatt caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag
      721 ttgataaaag aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa
      781 atttttgatg ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat
      841 tttgaccaac ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat
      901 gatcagccga tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct
      961 ttggaaaaac gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt
     1021 ggagaagctg gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa
     1081 tattactttg tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa
     1141 tacgattgtg atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca
     1201 ggaaaaatgt gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa
     1261 tcgggtgatc ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac
     1321 tacaatcgag cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt
     1381 gttcgcagac aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa
     1441 atataatggc tgcggaaatg attttatctt attagattac gcagaagaca cagattatag
     1501 tcaattagcc gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt
     1561 aaaaatcgat ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg
     1621 cggaaatggc attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca
     1681 ggaattcaac gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc
     1741 tttttattgc agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa
     1801 tcaaacttat ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac
     1861 tagtttattt ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt
     1921 ctggagaaaa agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt
     1981 caactttgta gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt
     2041 aggttggaca cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac
     2101 tgggaaaatt tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc
     2161 tggtgaggat gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga
     2221 ggaatgaaga tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga
     2281 tcagtagatt atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat
     2341 ggattattag ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag
     2401 cagatccttc aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct
     2461 ggaacgaata atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca
     2521 gacgcacttt tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct
     2581 cattttacag caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct
     2641 agaacaggca tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc
     2701 attggtttga aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt
     2761 ggagaagaat tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta
     2821 ggaggaagcg gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta
     2881 gtaaaagata cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt
     2941 gaactgatcc atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca
     3001 ttattagact taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa
     3061 aagaaacaat tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac
     3121 tgattggcag tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata
     3181 cggtccttct tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc
     3241 cagaactgct tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct
     3301 tatcatatca acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag
     3361 aaataaaaga acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca
     3421 tcatggtgat gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg
     3481 aactgattga aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc
     3541 tgcttcaaac aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa
     3601 aaagagaaga aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac
     3661 atgatgtatt gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta
     3721 atcgaatctt tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag
     3781 gttattacaa attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca
     3841 agaaatcatc cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa
     3901 tgtaaccgag ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt
     3961 tgtttttggt gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat
     4021 tgaaatcgaa atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa
     4081 aattaatgct cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa
     4141 tgccgtgatc atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat
     4201 gattgatatg ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg
     4261 tgcaggagct gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga
     4321 agacggcgta ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga
     4381 tgcagtcgtg gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg
     4441 tggaatacct gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt
     4501 gattgctgcg ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg
     4561 tattgtgggg gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga
     4621 ttttccagta ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt
     4681 attcaagaaa aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga
     4741 tttagcgcta ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag
     4801 tcagggacta tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt
     4861 aatcgttcca gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa
     4921 ttgttcaacc attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca
     4981 gcgagtcaat tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga
     5041 tcttcatcgt acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac
     5101 ggtgattccg gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat
     5161 gataaatgag acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt
     5221 acgtgtgcca atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt
     5281 tacgacggaa gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga
     5341 tctagcagca cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt
     5401 tgggaggatc agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga
     5461 taacatacgc aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa
     5521 aatagcggag gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg
     5581 atgtctcatg aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc
     5641 caagtgatcg ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac
     5701 ttactttatt tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa
     5761 aggatttgtg atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa
     5821 gatgaactag cacgcataca gaaacaactg gaagacgaaa agataactga agattattta
     5881 gtaagtagag gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt
     5941 attgacgcaa aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca
     6001 gcacaggaaa tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac
     6061 ggggcgaacc ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc
     6121 gcgatactgg cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga
     6181 atcatgatgg ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt
     6241 agagaactga gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc
     6301 ccagttcaag aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa
     6361 gggacaaaaa tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga
     6421 cgcaaggatt ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt
     6481 atctggaaag caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca
     6541 gggatcgaca atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc
     6601 atcacgaaag aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta
     6661 gctttgatct ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg
     6721 ttatcgatat tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag
     6781 ttgaatctga tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat
     6841 gaagggatgg tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga
     6901 aatcgggttt catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg
     6961 ggccgttatc catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga
     7021 agaaacgatt gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct
     7081 ctcttttgct tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc
     7141 gatgctttta gggttgactg actttataac taaacacatt gaggatctta tttataatgt
     7201 agtactgatc tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc
     7261 aggattgttg gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact
     7321 agctaaggga gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga
     7381 cattatcatt aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct
     7441 ggaggcagct gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga
     7501 gtggaatcat ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc
     7561 tggagaagtt attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt
     7621 gaagagggga ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg
     7681 ctataatgac ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga
     7741 ttcaaacaaa ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata
     7801 tacgcaacaa tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca
     7861 tgatccgacg ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac
     7921 gattcttttt acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt
     7981 aaataatgat gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa
     8041 aaatacgaca attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg
     8101 gagaaccgga ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag
     8161 aagacttttc tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta
     8221 tatttcagca acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag
     8281 ttggtgcaac ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg
     8341 ttcttattcc agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg
     8401 aattgatccc gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac
     8461 gagcatttga acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc
     8521 ctactgggac gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat
     8581 atccggtatt tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata
     8641 tgtccatagc tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac
     8701 atgcgatgac tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac
     8761 taattaaagt ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg
     8821 ctgaagcgct aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac
     8881 gaagagacta tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg
     8941 gtgcatttta cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt
     9001 gtgtagaatt agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag
     9061 aaggagaagg ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct
     9121 gcaaacggat caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa
     9181 tcgttaattt tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg
     9241 aacgcgtttc ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt
     9301 ttaaaagaat attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg
     9361 catctcatgg agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag
     9421 atatcacgct atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt
     9481 atttagaaga caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc
     9541 cgcaatccaa tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa
     9601 aaacagtaac ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat
     9661 tgattttgag ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa
     9721 atgtctactt gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc
     9781 cggctattaa aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag
     9841 caagccgaaa agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca
     9901 cttatttaga tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg
     9961 aagttttgac aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca
    10021 aaacaagtga aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa
    10081 atacagaatg gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg
    10141 aatacttgca aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt
    10201 ctatcccagc acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac
    10261 gttatgtagt aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag
    10321 gttcctctgc attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta
    10381 cagcagcaaa agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa
    10441 aatatgtacc agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa
    10501 tgacccatca attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa
    10561 atgtattggc actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact
    10621 tcttcttcca gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag
    10681 cgttaggaga agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa
    10741 aaagctgcct tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta
    10801 acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag
    10861 ggactgtgac acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg
    10921 gaggtcggta ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga
    10981 ttatttggat ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa
    11041 atgcttaaaa ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta
    11101 agaaaccgcg gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga
    11161 ataaaagtaa cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAZUKZ010000084        10878 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000084 DAZUKZ010000000
VERSION     DAZUKZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.086"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..281)
                     /locus_tag="ACG8EF_002088"
     CDS             complement(<1..281)
                     /locus_tag="ACG8EF_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4947381.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
     gene            453..1541
                     /locus_tag="ACG8EF_002089"
     CDS             453..1541
                     /locus_tag="ACG8EF_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4947382.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1669..3045
                     /locus_tag="ACG8EF_002090"
     CDS             1669..3045
                     /locus_tag="ACG8EF_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS4947383.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3446..5026
                     /locus_tag="ACG8EF_002091"
     CDS             3446..5026
                     /locus_tag="ACG8EF_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFS4947384.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5224..5447
                     /locus_tag="ACG8EF_002092"
                     /pseudo
     CDS             5224..5447
                     /locus_tag="ACG8EF_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5652..5972)
                     /locus_tag="ACG8EF_002093"
     CDS             complement(5652..5972)
                     /locus_tag="ACG8EF_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS4947385.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6120..8354)
                     /locus_tag="ACG8EF_002094"
     CDS             complement(6120..8354)
                     /locus_tag="ACG8EF_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4947386.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8833..9099
                     /locus_tag="ACG8EF_002095"
     CDS             8833..9099
                     /locus_tag="ACG8EF_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFS4947387.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9099..10826
                     /gene="ptsP"
                     /locus_tag="ACG8EF_002096"
     CDS             9099..10826
                     /gene="ptsP"
                     /locus_tag="ACG8EF_002096"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS4947388.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3462 a         1907 c         2208 g         3301 t
ORIGIN      
        1 gcttcttcaa tcaatatttt tcctaaccct tggtgtctgt attcagaaag aatcgccatc
       61 cgctgtaatt tcatttttcc gttttctaaa ggcaaaagac gtacagttcc ttgcggttct
      121 tttttatctg agtacaggac gaagtggata caatgggctt catctttgtc tatttctcga
      181 gacaaaggca caccctgttc gactacaaaa acttgatgcc tgatccgtac agcatctaag
      241 taaatattgc tcattgtatc ttttgtttgt acaactttca tggtctagcc accttccttt
      301 cgtatagtat actggaaaat aaaacaaata aaaagtatgg caagttttca aaaataaaaa
      361 aagaataaaa gaatttttaa cgaatataat atatactagt aagaaagaaa ggaggaaatc
      421 tattgtttga aaaacttaga cagtctaaac taatgttttg gtcgcttgaa ttattgatca
      481 tagctgcatt gatcatgatt tcatcagaaa taaactttat tttcgaaccg atcgggacat
      541 ttttttccac gttatttgca cctgtactga ttgcaggctt tctttactat ttattgaatc
      601 ctattgtcaa gctgttgatg aaagcaaaaa tcaaaagaat atgggctgtc gcaattgtgc
      661 tgctcctttt agttgcagca attatttgga ttttacttag tgtcatccca agtctagtac
      721 agcaaatttc ctcattggca tctaatatgc ctgatttcat caaacaggta gaaagctggt
      781 taaaagaggt agccgaattg cctttgttca aggaagtaga tatcaataaa tattttgagc
      841 aattagacat ttcttacggg acaatcattc agcaattcct tagtggagta tccaatagtt
      901 taggttcgat cgttggaacg atcgcttcgg caaccgtagt aatcattaca gcaccattca
      961 tcttgttcta tatgttgaaa gacggagaaa aactagtgcc aaacatccaa cagtttttcc
     1021 cagaaaaaag aagaaaccaa attgtggagt tattggggca actgaataaa acattggcta
     1081 attacattag cggacaagca atcgaatgtt tattcgttgg taccttcaca tttttaggat
     1141 atatggtaat tggtgtggat tatgctttct tatttggggt cattgcggga ttgacaaacc
     1201 taattccata tttaggacct tatctaggac tagcacctgc atttctagta acagtcttca
     1261 atgatccggt aaaagcatta ctttgttgtg tggttgtgct aatcgtccaa cagttggatg
     1321 ggaatatcat ttatcctaat gtaattggga aatcactgaa aatccatcca ttaacgatca
     1381 ttatcgttct tctagtagct ggaaatattg ccggattgct tggtatcttc ttaggtgttc
     1441 ctttttacgc aatctgcaag acgatcattt cctatataat caaaatcgtc aaagaagaca
     1501 agcaaaatga aaacaagaag aaaattgcta cgacctcgta ggtagtttta gtacacttgt
     1561 ttttattgca aagaaaattg ctttgtgata ccattttaac gtggctatag tgccacgttt
     1621 ttttctatcg taaactaaaa attaatttta taaggagaga atgcgattat gaatgctgac
     1681 cctgagagtc agtcgctgtt agcgcagatt ttattattga tcgttttgac attgatcaat
     1741 gcttttttag cagcttccga aattgctgtt gtttcgatca ataagaatcg aatcgagcaa
     1801 aaagctgatg aaggagatgt gaaatctcaa aagcttttaa aaatattgca gaatccaaat
     1861 aatttcctat cgacaatcca agtaggaatc actttggtca acattttgtc tggtgcttca
     1921 ctagctaata ctttatcgga acggttagct ccggtattag gtggcggcac agcggcaaaa
     1981 aatattgcaa atatcattgt gctggctatt ttgacctatg tatcgattgt ttttggagaa
     2041 ttgtacccta aacgtattgc tttaaataaa tcagaagaag tcgcaaagtt cacttcaggg
     2101 ctgatacgct tgattggtgt agtagctaag cccttcgtct ggctgctttc tgcttccaca
     2161 agtttattgg cgcgtatcac gccgatgact tttgatgatg aagattcaaa aatgaccaga
     2221 gatgaaatgc gttatatgct tgaaaacgaa ggcgtattga ataatgaaga attagaaatg
     2281 ctccaagggg ttttttctct tgatacgaaa gttgcccgag aagtcatggt cccacgaacg
     2341 gatgcattta tgattgatat caacgacact gtggaagaaa atgtgaatga agttttatcc
     2401 gaaaactatt caagaatccc tgtttataac gaagacaaag ataaagtagt cggaatcctt
     2461 catacaaaga atttactaaa agctgcccat aaattcggct ttgataattt agatatcaag
     2521 aaaatcatgc aggagccttt attcgttcct gaaacggtct ttattgatga tctgttatat
     2581 gaaatgaaaa agacgcagaa tcagatggct attttattag atgaatatgg cggcgtcgtt
     2641 gggttagcaa cactagaaga ccttttagaa gaaatcgtcg gagaaatcga tgatgaatca
     2701 gatgaagttg agaatcttta tgaaaaaatc gatgaacacg aatatatcat ccaaggacgt
     2761 atgctgattg atgagttcaa tgaagctttt gacagtgatc tgcatatgag tgatgtagat
     2821 acgatggctg gttatttgat tacagcatta ggcatgattc cggatgaagg cgaaaagtta
     2881 tcgtttgatg tcgataatat tacgctggtt tctgaagaaa tggaaggttc tcgaatcctt
     2941 aaaatccgtg tcatttttca tgatccggaa gaaacagaag cggaacctga agaagaacga
     3001 agatacttca gaaaagaatt cgaagatgac gagccaagaa gataattgga aacttggtta
     3061 tgaaagacgt gttgcttgaa taaacaagca acacgtcttt tcttttagtc agctgacaaa
     3121 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
     3181 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
     3241 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
     3301 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
     3361 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
     3421 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
     3481 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
     3541 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
     3601 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
     3661 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
     3721 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
     3781 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
     3841 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
     3901 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
     3961 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
     4021 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     4081 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     4141 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     4201 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     4261 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     4321 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     4381 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     4441 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     4501 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     4561 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     4621 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     4681 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     4741 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     4801 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     4861 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     4921 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     4981 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     5041 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     5101 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     5161 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     5221 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaagaa gagatgttac
     5281 gattatgtcg attattagat gtaaaagact gggacaaaaa cgagcaaatt gcacaaaaag
     5341 tacgatcaat catccataaa gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa
     5401 atgaaaagag aaaagtacaa aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg
     5461 ctgagacaga agcgtttaac tccaagaaat aagctggaat tcacaaaaat ttgaaaagca
     5521 attttcgtga atttcagctt atttccgaag gagttgcttc tgctttcgcc gtttatacgt
     5581 gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctcttta attattatgt
     5641 tcaaaatttg tttacacggc taatgtagcg cctaattcat gttttggaat cgagatttca
     5701 acaaggcctg ccaattcgat tactttgatt tcatcctcgt ggtaaacggc gtctttgatt
     5761 ttatctaaga aataattttt agcatcttcg atttctttcg gctggatttt acgtttgtca
     5821 ttgatcagca gaatttcatt atgttcaggg gcttcagtgt agacaatagt ggtatttccg
     5881 gctgtgtaga ccttgactag atgggcatca gtgttttcaa gctggtttaa tacaagacgg
     5941 gaatggctgt ttgttacatt taccaatttc atggcgctca cctcttttat gtatttttaa
     6001 ttgcttgttt ctcttattat aatttctttt ttataaaatg aacagtattt tgacttttct
     6061 gaaaaaagaa tgagacggca tttctcaaag aaataccgtc tcacgctata aaactggatt
     6121 tattcagccg tttcttcggc tgattcttta gccaaacgtt ctggtttaga agtaacgatg
     6181 atttttccat ctttatccag ttttgctttc aactcatgga tagaagggtg atcaagataa
     6241 aattcagcga taccatcttc gatttgttct tggatcgtgc gtcgaagagg acgagcaccc
     6301 atagatggat catagcctaa gtcaactaat ttttctttga cattcgtagg aacttcgatg
     6361 tgaagttgct gtgctgccag catttggttg acatcgtcta gcattagtgt gacgattgtc
     6421 atcaagtttt cttttgataa tgcactgaat tcgatgatcc cgtcaaaacg gttcagaaat
     6481 tctggtgtaa agtagttgtt taattggttc aatacagaac gagtaacacc ttcgcgtgcc
     6541 gcaccaaaac caacgtttgc ttctacttta ccagttccgg cattacttgt cataatgatg
     6601 atcgtatctt taaagctgac cgttcttcct tgtgcatctg tcaatcgacc atcatctaag
     6661 atttgcaaga acatatgcaa aacatctgga tgagcttttt ctacttcatc taacaagaca
     6721 agactgtaag gatttctccg tactttttct gttaattgac cagcttcttc gtatccgaca
     6781 taacctggag gagaaccaat caatttagag acactatgtt tttccatata ttcagacata
     6841 tcgaaacgaa tcattgaatc ttgtgaaccg aatagttcgt aggcaagttg tttcgcaagc
     6901 tctgttttac ctacaccagt tggtccaaca aataagaagg agccgattgg acgattctgt
     6961 ttgtttaatc caacacggtt tcggcgaatc gctttcgcta ctcgatctac agcgttatct
     7021 tgtccgatga catgagcttt caagtcatct gctaagtttt tcagttgtgt ctgttctttt
     7081 tcttttaatt cacccactgg aatacctgtc cgttgttcaa cgattttttc catatctttt
     7141 tcggtaatca caggtgtttc ctcttcagtg agctgacgtt ctttcatttt ttgaagttta
     7201 ttgatctgat cgcgataata agctgctttt tcgaaatctt cttctcgaga agctaaaact
     7261 ttttgttctt ctgcttcttg cagttttttc tcgattgttt taggatcaac gatttggatc
     7321 gtcaagtttt tcttagaacc tgtttcatct agcaaatcga tggctttatc cggcaagaag
     7381 cgatcttgga cgtaacgatt ggataaagtt gcggctgctt cgatggcctc atcagtatat
     7441 tttacatgat gatagtcttc ataacgtttt tgcaaacctt tcaaaatact gatcgtttct
     7501 tcgacagttg gttcatctac ccgtacaggt tgcatacgac gttctaaagc agcgtctttt
     7561 tcgatgatgc ggtactcatt taaggtagta gcaccaacca tttgcaattc tcctcgagct
     7621 aaagctggtt ttaaaatatt accagcatcc atatttccat caccagcaga accagcaccg
     7681 acgatttcgt gaacttcatc tatgaatagg atcacatttt ctgcttgacg gatctcttca
     7741 atcaattttt gcatacgttc ttcaaattgt cctcgtatac cagtaccttg gactaaagat
     7801 acgacatcta aacgaatgac ttctttgtcc attaattttt gcggtacatc accatctacg
     7861 attttttgtg ctaaaccttc aacaactgct gttttaccaa cacctggttc tccaatcaag
     7921 acaggattgt tcttggttcg gcgatttaaa atttcaatga ctcgtttgat ttcgtcatcg
     7981 cgtccgatca caggatcgat atcaccttgt cgtgcttgtt ctgtgatatt gattccgtat
     8041 tctcccaata aaccttctgt attgccttga ggtggctgac caccattgaa gttgttgttt
     8101 ccgccaaatt gtgttggtgg tgtttgctca taatttcctt gctgttgcat ttggcgagac
     8161 atggaacgga aaagatcgtc caaactgcca aatccaaatg gatcattttg ggccatatta
     8221 gtcagacctc cattttgttg atttttgatt ttttgataac agctctgaca gtaatcgagt
     8281 tgtgtccgct gaccattgac agtggcatac aagtgaatcg ttgcctcatt cttgccgcag
     8341 ttttgacaaa gcatgaatca ttcacgtcct ttcacaaaaa tgggtacatc catattttac
     8401 atctcgtttt tcatgaggta aaagaataac ccctagtttt ttaagagatt tatgaggtag
     8461 aaaaggtgaa atctctattg accattcctg acctttgact cttattatac taaaaataaa
     8521 aagggaagca agtaattcgt ttcatatgaa aacaaagagg gtcaaaggaa agaatgataa
     8581 acgtcaggag aagagaaagc gacaagaaaa aggaaataaa cacgtttgtt ataaaatgtg
     8641 aagaaaagga tgaaaaagaa tccggaataa tttgaatact tttatctgaa attgaatgaa
     8701 aactgttgta tctaacgaaa aaaaatgtta atctttacag atgaaagggt ggcatttgtt
     8761 aggattctct gaattactaa aaaagaaaac cctcacataa aaacaacact cacttaaagg
     8821 agaccgatta atatggaaaa gaaagaattt cacgtagtag cagaaactgg gatccacgct
     8881 cgtccagcta cactattagt acaaacagca agcaaattta actctgatgt aaacttagag
     8941 tataaaggta aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt
     9001 caaggttctg atgttactat tactgcagaa ggtgctgacg aagctgatgc aatggcagca
     9061 atcgttgaaa caatgaagaa agaaggctta tctgaataat ggttgaaatg ctaaaaggga
     9121 ttgccgctag tgacggtgta gccgttgcaa aagcttacct gctagttcaa ccggatttaa
     9181 cattcagtaa ggcaacagta gaagatactg cggctgaaga agctcgtttg gatgctgcat
     9241 tagctaaatc tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg
     9301 aagcagaagc gcaagtattt gatgcccatt tgatggttct ttcagatcca gaaatggtag
     9361 gtcaaatcaa gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag
     9421 taacggatat gtatatcggt atgttcgaag caatggaaga taatgcttat atgcaagagc
     9481 gtgctgcaga tattcgtgac gttgctaaac gtattttagc gcacttatta ggcgttactt
     9541 taccaaatcc atcaatgatc aacgaagaag tggtcgttgt tgcacatgac ttgactccaa
     9601 gtgatacagc acaattagat cgcaattttg taaaagcgtt tgtaacagac attggtggac
     9661 gtacttcaca ttctgctatc atggcacgtt ctttagaaat tccagcaatc gttgggacaa
     9721 aagaaattac tgctaaagtc aaagaaggcg atattttggc tgtcaacggg atcgaaggcg
     9781 atgtaatcat cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt
     9841 acgcagcaca aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc acagctgacg
     9901 gcaagcattt tgaattagct gcaaacatcg gaacaccaaa agatctagtt ggtgtccata
     9961 ataatggtgg agaagcagtt ggtctgtacc gtactgaatt cctgtatatg gattctccag
    10021 acttcccaac agaagatgac caatatgaag cttacaaagc tgtattagaa ggtatggaag
    10081 gaaaacctgt agttgttcgt acaatggaca tcggtggaga taaagaacta ccatatctgc
    10141 aattacctca tgaaatgaat ccattcttag gatatcgtgc attgcgtatc agtttatctg
    10201 aacaaggcga cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg
    10261 gtaatttacg tatcatgttc cctatggtag ctacactcaa agaattccgc gcagcaaaag
    10321 cgatctttga agaagaaaaa caaaaattga taagtgaagg caaggaagta tctgatacga
    10381 tccaagtcgg catcatgatc gagattccag cagcagcagt cttagcagac aagtttgcaa
    10441 aagaagtaga tttcttctct gtaggtacga acgatttgat tcaatacaca atggcagcag
    10501 accgtatgaa cgaacgcgtt tcttacttgt accaaccata taacccatca attttacgtt
    10561 tgatcaaaaa tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag
    10621 aaatggctgg agatcaaaca gctgttccat tgctagtagg tatgggtcta gatgagttct
    10681 caatgagtgc gacatctatt cttaaaacgc gtagtttaat gaaacgcttg gacacagcga
    10741 aaatggctga acttgctgat cgtgcattga aagaatgcga tatgatggaa gaagtcgttg
    10801 aacttgttca tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag
    10861 agctacgcca gttttttt
//
LOCUS       DAZUKZ010000085        10726 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000085 DAZUKZ010000000
VERSION     DAZUKZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.087"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..1091)
                     /locus_tag="ACG8EF_002097"
     CDS             complement(243..1091)
                     /locus_tag="ACG8EF_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4947389.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1255..2157
                     /gene="gnd"
                     /locus_tag="ACG8EF_002098"
     CDS             1255..2157
                     /gene="gnd"
                     /locus_tag="ACG8EF_002098"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFS4947390.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2159..3160
                     /locus_tag="ACG8EF_002099"
     CDS             2159..3160
                     /locus_tag="ACG8EF_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFS4947391.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3157..3441
                     /locus_tag="ACG8EF_002100"
     CDS             3157..3441
                     /locus_tag="ACG8EF_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFS4947392.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3474..4271
                     /locus_tag="ACG8EF_002101"
     CDS             3474..4271
                     /locus_tag="ACG8EF_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4947393.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4285..5118
                     /locus_tag="ACG8EF_002102"
     CDS             4285..5118
                     /locus_tag="ACG8EF_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4947394.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5136..5993
                     /locus_tag="ACG8EF_002103"
     CDS             5136..5993
                     /locus_tag="ACG8EF_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4947395.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6015..6524
                     /locus_tag="ACG8EF_002104"
     CDS             6015..6524
                     /locus_tag="ACG8EF_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS4947396.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6561..6962
                     /locus_tag="ACG8EF_002105"
     CDS             6561..6962
                     /locus_tag="ACG8EF_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947397.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7074..7427
                     /locus_tag="ACG8EF_002106"
     CDS             7074..7427
                     /locus_tag="ACG8EF_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947398.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7461..7904
                     /locus_tag="ACG8EF_002107"
     CDS             7461..7904
                     /locus_tag="ACG8EF_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4947399.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7921..9006
                     /gene="uxuA"
                     /locus_tag="ACG8EF_002108"
     CDS             7921..9006
                     /gene="uxuA"
                     /locus_tag="ACG8EF_002108"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4947400.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8999..9712
                     /locus_tag="ACG8EF_002109"
     CDS             8999..9712
                     /locus_tag="ACG8EF_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4947401.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9919..10068)
                     /locus_tag="ACG8EF_002110"
     CDS             complement(9919..10068)
                     /locus_tag="ACG8EF_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947402.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10464..10664
                     /locus_tag="ACG8EF_002111"
     CDS             10464..10664
                     /locus_tag="ACG8EF_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4947403.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3589 a         1696 c         2320 g         3121 t
ORIGIN      
        1 acaaaaataa taaaaaaaag aaaagataaa ttttttacta ctgtatacta actttgttat
       61 cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat
      121 acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac
      181 taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg
      241 tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct
      301 ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata
      361 cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca
      421 tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca
      481 gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc
      541 aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc
      601 gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca
      661 atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc
      721 gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta
      781 gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg
      841 atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat
      901 ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca
      961 cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg
     1021 gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg
     1081 gagacgctca tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt
     1141 aatgttaaag atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct
     1201 atttacaaaa aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa
     1261 attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac
     1321 catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat
     1381 gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc
     1441 atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca
     1501 aaactcggac caggagacat tattattgaa ggcggaaact cttattataa agattcaatc
     1561 cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga
     1621 ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt
     1681 ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt
     1741 gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg
     1801 caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa
     1861 caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg
     1921 gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca
     1981 ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt
     2041 gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta
     2101 gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat
     2161 gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca
     2221 cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc
     2281 aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc
     2341 attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg
     2401 tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat
     2461 catacctgct ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc
     2521 catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg
     2581 ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa
     2641 taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta
     2701 tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca
     2761 agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta
     2821 tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag
     2881 tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat
     2941 cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt
     3001 agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga
     3061 agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga
     3121 acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt
     3181 ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt
     3241 tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca
     3301 tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat
     3361 catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa
     3421 aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt
     3481 atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg
     3541 ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag
     3601 aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc
     3661 ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc
     3721 tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg
     3781 cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac
     3841 aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt
     3901 tagttggagg attaggagat aaatacgcaa gaaatgcggc agcacatgct atgcatgacg
     3961 ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg
     4021 ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt
     4081 tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata
     4141 aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc
     4201 ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca
     4261 gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt
     4321 gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta
     4381 cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa
     4441 gcgatgattg ttacagccgc tttgcaaatg atttatatgg ggggtttttc tccaggagga
     4501 tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg
     4561 gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac
     4621 ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta
     4681 gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca
     4741 tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct
     4801 gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga
     4861 gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg
     4921 gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg
     4981 gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg
     5041 aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac
     5101 tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa
     5161 caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt
     5221 ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa
     5281 tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac
     5341 ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa
     5401 tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc
     5461 aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag
     5521 atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac
     5581 aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt
     5641 tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa
     5701 tgcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga
     5761 tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg
     5821 gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag
     5881 ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat
     5941 tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag
     6001 ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg
     6061 ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg
     6121 aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag
     6181 gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat
     6241 ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa
     6301 aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata
     6361 cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag
     6421 aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca tctgcaccta
     6481 aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa
     6541 aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt
     6601 atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat
     6661 tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag
     6721 cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta
     6781 cttattacga tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc
     6841 aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt
     6901 ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat
     6961 aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc
     7021 gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag
     7081 aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt
     7141 atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt
     7201 atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata
     7261 aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta
     7321 gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa
     7381 catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa
     7441 aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg
     7501 aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt
     7561 gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta
     7621 tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca
     7681 atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca
     7741 tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac
     7801 cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc
     7861 atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag
     7921 atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt
     7981 cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat
     8041 gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa
     8101 ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga
     8161 gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga
     8221 ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa
     8281 aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca
     8341 ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa
     8401 gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa
     8461 gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca
     8521 gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga
     8581 gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat
     8641 ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc
     8701 atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga
     8761 gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat
     8821 attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga
     8881 accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc
     8941 acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat
     9001 gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt
     9061 agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt
     9121 ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta
     9181 cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat
     9241 cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc
     9301 aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat
     9361 gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt
     9421 aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc
     9481 tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc
     9541 gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa
     9601 gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta
     9661 tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg
     9721 actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga
     9781 agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca
     9841 aaacgcatct actgttatct accagtagat gcgtttaaag agaagaacga gaatcgtcca
     9901 tatgagggtt tccactcctt atgcagattt ttttaattct tgcactgctt gttcgatcgt
     9961 aacacctgtc gctacatgat tggtattttc tttgtcaaaa acgacaaata gattcaaatc
    10021 tgtatccaag gttactcgat attgtatatc tttgttatag aatgtcatag ccattctcct
    10081 ttctttaaat acttgctgaa aggcaacgaa cgcgttgata acgaaattcc atttataagt
    10141 ataccaccaa ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag ttgcagataa
    10201 aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga
    10261 ttcgaagaca atagaagaca atgaatttta tatttcattg cattatcctt gtgttattag
    10321 aaaaagcatg atatattaaa tgagtaatct attttgaaac gtaatttgag cgatatatct
    10381 acatagataa aactcctttt gcctatcaaa tatttattgc aacgaaaaaa cgtacattga
    10441 tacgtatcag gaggaattta tttatgaata acggtacagt aaaatggttc aacgcagaca
    10501 aaggttttgg ctttatcact ggtgaagatg gaaatgatgt atttgcacat ttttcagcta
    10561 tccaaggtga cggcttcaaa actttagaag aaggccaagc agtgtcttac gatgttgaag
    10621 atggtcaacg tggccctcaa gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta
    10681 accgtctttt cgaaagaaaa gacggttttt ttgtacgaaa aaattc
//
LOCUS       DAZUKZ010000086        10659 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000086 DAZUKZ010000000
VERSION     DAZUKZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.088"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..657
                     /locus_tag="ACG8EF_002112"
     CDS             34..657
                     /locus_tag="ACG8EF_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947404.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /gene="trpX"
                     /locus_tag="ACG8EF_002113"
     CDS             1107..2156
                     /gene="trpX"
                     /locus_tag="ACG8EF_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4947405.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="ACG8EF_002114"
     CDS             2153..3034
                     /locus_tag="ACG8EF_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4947406.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="ACG8EF_002115"
     CDS             3037..3807
                     /locus_tag="ACG8EF_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4947407.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="ACG8EF_002116"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="ACG8EF_002116"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFS4947408.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="ACG8EF_002117"
     CDS             5118..6281
                     /locus_tag="ACG8EF_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4947409.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="ACG8EF_002118"
     CDS             6398..6808
                     /locus_tag="ACG8EF_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4947410.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="ACG8EF_002119"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="ACG8EF_002119"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4947411.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9381..9665
                     /locus_tag="ACG8EF_002120"
     CDS             9381..9665
                     /locus_tag="ACG8EF_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947412.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9825..10061
                     /locus_tag="ACG8EF_002121"
     CDS             9825..10061
                     /locus_tag="ACG8EF_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4947413.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10058..10456
                     /locus_tag="ACG8EF_002122"
     CDS             10058..10456
                     /locus_tag="ACG8EF_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS4947414.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3724 a         1773 c         2253 g         2909 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
     9661 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
     9721 ttcgtaagaa aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata
     9781 atgtacttac tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa
     9841 agtataaaat tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag
     9901 aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg
     9961 tacgaatgat caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga
    10021 atcaatatga agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga
    10081 aattatcaaa atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt
    10141 aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca
    10201 agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa
    10261 acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca
    10321 attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat
    10381 taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca
    10441 aaccgcaata aaatgaacga attaaaaact ttatttctca attagatcag catgtcatta
    10501 aggaatatgc tgattttttt atgtttaatc atacaaaata gttttttcat aaaaatatgg
    10561 aaaatatgga ttaattttga aataattgat ttaaaaggaa tacaaaagag tgaatagtag
    10621 gaaatagtgg tttagaacta gtgggctgat tttttatat
//
LOCUS       DAZUKZ010000087        10644 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000087 DAZUKZ010000000
VERSION     DAZUKZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.089"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            58..897
                     /locus_tag="ACG8EF_002123"
     CDS             58..897
                     /locus_tag="ACG8EF_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HFS4947415.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            1048..4065
                     /locus_tag="ACG8EF_002124"
     CDS             1048..4065
                     /locus_tag="ACG8EF_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4947416.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            4129..5592
                     /locus_tag="ACG8EF_002125"
     CDS             4129..5592
                     /locus_tag="ACG8EF_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4947417.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            6059..6439
                     /locus_tag="ACG8EF_002126"
     CDS             6059..6439
                     /locus_tag="ACG8EF_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFS4947418.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            6436..6705
                     /locus_tag="ACG8EF_002127"
     CDS             6436..6705
                     /locus_tag="ACG8EF_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947419.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            6705..7625
                     /locus_tag="ACG8EF_002128"
     CDS             6705..7625
                     /locus_tag="ACG8EF_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947420.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            7647..8519
                     /locus_tag="ACG8EF_002129"
     CDS             7647..8519
                     /locus_tag="ACG8EF_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS4947421.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            8538..9710
                     /locus_tag="ACG8EF_002130"
     CDS             8538..9710
                     /locus_tag="ACG8EF_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFS4947422.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            9721..10371
                     /locus_tag="ACG8EF_002131"
     CDS             9721..10371
                     /locus_tag="ACG8EF_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947423.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            10387..>10644
                     /locus_tag="ACG8EF_002132"
     CDS             10387..>10644
                     /locus_tag="ACG8EF_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947424.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
BASE COUNT         3918 a         1800 c         2263 g         2663 t
ORIGIN      
        1 ataagtttgg tactatgttt ttgtaaataa atttattcaa atcggaggcg aaaaacaatg
       61 aaaatgttga aagtattggc actaggaatc gtagtcggag gagttagtct tgctgtggga
      121 acgaatgctg aagcagcaga aaacaaagtt tggacacctc gttcggcgga acaaatcaaa
      181 gcagaaatta aagggaatga atacacaatt gtctggggag ataccctaag cgcaatcagt
      241 caagcaacaa atattactgt ccaaaaatta gcggacatga ataagattac aaatgtggat
      301 ttaatctatg caggaaataa attagtattt aatggcaatg tagcaagtgt acaaaacagc
      361 gcaggtgaaa ctgtggctca aacagtgatt caagagaaag acaaagttga tccgaatcaa
      421 ccagtaggac aaggaacgac ttcaaataca caaacgacac caaatacaac gacaccaaca
      481 acaccagctg gagatcaaac aggaacaact ggtggtacaa caacgccaag tacaccagaa
      541 aacaatggtg gcactactca aacacctaca caaccaggta atggtggaaa tactggagga
      601 gaaacaactc cttctaaccc aagcacaggt ggaggaacaa caacgcctga agaaccaagc
      661 aatcctggtg gaggcgatca aggcggaaat ggtggcacaa cgacacctga agagccaaca
      721 attgtagttg gagagattgg taatagtgga aaggtattta acactgtaga tgaagcctat
      781 gattgggcta atgcagaggt tagcgatcca aattcgcagt ggtacaacaa aggttttatg
      841 gtggatgtta ttatttacag tgacgggtca caaaaacaca ctgtagattt ctactaaaaa
      901 taaaagaaaa gtaagtggtt gatgaagact agaattataa gttctagtct ttttttgttt
      961 tgaggaggat aaaaatgttt cggtgtcgaa gtccataaga aattacttta gaagactgaa
     1021 aaataaaatg aaaggaacga aaaaaatatg tttaaaagaa tggcaacttg gttggcaatt
     1081 ttcgttacat ttctcaatcc catgttagga tctgtaacgg catttgcaga aacgataaat
     1141 gaatcaaatt ctactgaaga agtaatgaag actgaagaat caaaaagtag tgaagaagcg
     1201 atcagtactg aaaccacaag tgaaacaaca gaagagacaa ctacgcagga cacgacagaa
     1261 gatgtttctc cgcccaatgt aatgactcaa gaggtccctg aaggttatga gaaatacttt
     1321 ataaccattg aagatattca aaaagcgact tctttttctg aacaagaacg attggcgcaa
     1381 gaatctgaag aatggcaagc aatcttggaa aagaatgcac agacacgtgc taccaatcta
     1441 acattgactg aaaatggtcg taaagatgtg aatatgactc gaattactcc tgaaggaaca
     1501 atgtatactc atgattacat ggttcgctgg caaattgacg gagaagatgt tttttgcatt
     1561 caagaaggcg caatcacgag tgcgggtatt caatatgatg tgacaccact cgaaaattta
     1621 attacgacaa gtgctattag aaaaaagatt tctttaattg gttattttgg ctactattct
     1681 agtggttcaa tggataacta tgtattagca caaacatata tttgggaagt tttaggtggt
     1741 tcatttgtta attatggaac tattggacaa tctaattatg aagctttcaa acagactgta
     1801 gatagaaaaa taaacaattt acttgaagat ccttcttttg atagccaaac agtgacggtt
     1861 aaagtagggg aagaagtaga agttaaggat acaaaaggtg tgttttctca atgggatggg
     1921 tcggttgttg aaaacacagc taacgttcaa gtaagaaaat caggaaatag tttgtttata
     1981 aaagcaaatc aaaactcaaa tgacggacga atttctgttt cttcactgga tccaaattct
     2041 agaggggtag gttcaagttt tgcctatgta gatcctgttg ctcaaaatct aggaaagctt
     2101 tatgtgccag atcctaatag agctggacta actattaaag ttctaaaaaa tggcgacttc
     2161 aaactaacca aactagacgc tgtcacaggg aaaaaagttc caaatgtgac gtatgaaatc
     2221 aaacgtcatg atacgggaga aacaaaacaa tatacaacgg atgccaaagg ggaaatcgcc
     2281 ttaaaagaaa tccctgcatt ctatgaaaaa gacggaaaat atgttcaaaa taaattggat
     2341 gtcaaagaag tcaaagcacc agctgggtat attctagaca aacaaactca tacgattacg
     2401 attgaaccaa acaaagaagt gacgttaact gcaaaaaatg acttacaaca gttcattcca
     2461 gaaggaaaga aagtcaaaga agtctataat gcggaagaat cctggaatca aggcaaacct
     2521 gtttatgata ccgttcctgc agaaaatatc gaatttgata ttatcgccaa aacagatatt
     2581 actgcgccag ataaagaaac agtccttgtg aaaaaaggag aagtcttgga ttcagtaaaa
     2641 actgacaaag acggacactg gaaggcaaac aaaaaattct acaatggcga tcaaaacaaa
     2701 taccaattga ttgaaaaaaa tgtgccagac aactaccgtc aaccttctga agaacaaacc
     2761 acgttctctg tggcctatgg cgataacacg acgcctgtgc ttacgtttga attagatgtg
     2821 attgacaact tgttaaaaac gggtgaagta gacttcaata aagtcgattc aatcaatcat
     2881 ctacaattac caggagcgaa attcctgatt gaaggtatga ctgcacataa taaaaatgtg
     2941 tcctttgtgt atgaaacaaa agaagaacca gaaaaactaa aattaccaga cggtacgtat
     3001 aaatttacgg aaattgtgtt ccctgagcat tatgggcaag cgtcaggaca aacagaaacg
     3061 attttagtaa cggtcaaaga cggagaagta caaccaatta ctgtgaaaaa cgaagaaatc
     3121 gtggttccag aagaagacca accagaaatt tctactttgt ttgtgacaac gacaggtgga
     3181 aaagatttca acccgaaaaa agaccaagaa ttagtggata aagtgtccca agtattccct
     3241 aaatctgcgg ttgggacaga gaaatattgg gtgacgcaat tccataaaat tactgccgat
     3301 ggaaaagtca ctgtattaga tacggtaaaa gaaacacgaa aagtgaccga tgaaaaagaa
     3361 acatttgatg tgacgttcaa ctacaaagca gacatggttt cagacggtga cacgattgtc
     3421 gcaacgcaca ttgtctataa cgacaaagaa gaggaagaag aattcgctcg tcattttgat
     3481 ttaaccaata aagatcaaac agtgactgcg aaagaaccaa aaatcgaaac acgctttatt
     3541 acagtgaatg gcgaccaaac atttgatcca gacaaagacc aagagttgat cgatcatgta
     3601 tcgcaagaat ttccagaatc tacggtaggg gaaaccaaat actgggtgac tcaattccat
     3661 aagattgata aaaatggaaa agatacagtc gttggtacgg tagaaactgc acgtaaagtt
     3721 tctaaagcgt ctgaagagtt taaagtatcc tttgactaca aagcaggcac gttaaaagac
     3781 ggggagaaat tggtcgcaac tcatattgtc tataaggata aagaccataa agaggaagaa
     3841 gcacgtcact ttgatttgaa gaatgaaaaa caaacattga cagcaaaagg cattaaaaaa
     3901 gaaacgccga caacccctgt caaacaaacg ccgacgagca caccaaaaca aagcttacca
     3961 aaaacaggag aaacagtgaa cgatgtgtta attgcattgg gactgtttct ttttgttggt
     4021 gtgggggctg ttctggttcg tcgtaaaatg aaacaaagca attaaagtat gactagagac
     4081 agcaatcagc tgtctctaga tgattgattg aagaatggag gcaatcttgt gaaaaaaacg
     4141 agtaaatgtt tatctgcttt gttattagta ggtcaagtat ttgggtatac gccagcggtt
     4201 ttggctaatg aacaaggcaa caatcaactg acaaattcgc aatctgcaac gatcactgaa
     4261 gaaacgacaa caaattccac tggagaagaa aatcaaccag aaacaccgac tgaagataca
     4321 atagatacac cagataatcc agaaatgaac gctccaccaa caggttctga agaacaacca
     4381 gaaacaccga atcaaccaga caaaccagaa gacaatcaac aacctgagga ggaaccgtca
     4441 gaagaaccaa atgagcaacc ttctgatgaa agctcacaga ctcctcaaaa accaagtacc
     4501 gaaacagaag cggaaccgcc aacaaaaacg gaacgcccac aaaaggcacc tgaacagagt
     4561 gtgactgtac ctatgaatga cgtaccgcag gcacaatcaa tcggttcaga agagacaaaa
     4621 aactaccacc aagcaccaag cgcaccgata caagaattag tcccttccag cacacaagat
     4681 aacgaaggga ctattcattt tgaaaaagac gcgtctgtag aaacgtttat ccgtaaaatc
     4741 ggtgaatctg ctcgtaaaat tggacaagaa aatgatttat acgcttctgt tatgatcgcg
     4801 caagccattt tagaatccgc aagcggacaa agtcaattgg ctcaggcacc aaactacaat
     4861 ctatttggaa tcaaaggcac acataacgga aagggcgtct cttttgcgac acaagaagat
     4921 ttaggcaatg ggacacttta tacgacccaa gcaacttttc ggcaatatga gagctatgaa
     4981 gaaagcttaa aagactatgc caaactgtta aaagaaggct tagttggaaa tacctctttt
     5041 tatgatggtg tttggaaaag taaggcaaaa acctatcagg aagcgactaa atttttaaca
     5101 ggaagatacg caacagacac tagctacgat aaaaaattaa atgggttaat cgaaacctat
     5161 gacttaatca aatatgataa agaagtagct ggtccacaac taagcaaaga aggctatatg
     5221 gtacctctaa aaaattattc aatttctagt tccttcggtt ggcgaggaga agaatttcat
     5281 agaggattag acatggcagc caatcaagga gaaccaatct atgcgagcaa ggcaggaacg
     5341 gtcatagaag cttcgtatca tactttgtca ggaaattatg tcacaattga gcatgaagac
     5401 ggaagtgtgg ctctttatgc gcaccaacag gaatattgcg tgaacgtagg acaaacagtt
     5461 gaacaaggac aaattattgg ttatgtgggt tcaacaggaa atagtacagg accacattta
     5521 catttagaac ttgccttaga tggtagttta acaaaagaca aattaatcaa cccagaaaca
     5581 gtcttattct aaagaataag gctgtttctg ataaggagtg gtatagaaaa tgtggttact
     5641 ttttaaatat ttgatatatt tgtatctctc attttcgttt tttaatttga aatctcgtat
     5701 ctatctagaa cgtagtttat taaatatagt attaggaata ttttttatcg tttgggtctt
     5761 cattgactta tttctttact caaacgattt ttattttatt tataaagaat ttaaaaatca
     5821 gtaattgctt ggagagttaa catatattgc ataaaaaggc aaagtatgta catggtattt
     5881 atcgtgttca tactttgcct ttttatggcg aaatttttta gaaaggagga aaaaattttg
     5941 ttatttgtaa gtggatttct agctggtggt gtattggcca ccttttttat ggcaagtttc
     6001 attgttagta aaacagaaga aactagaatc aaaaataaac attagaaagg acaaagatat
     6061 gatcacaaat tataaaaaat tttggcagaa tagctttaat tacaaaggga aagccacaag
     6121 aaaagagtat tggctcgttg cgttaatgaa tgccctcatt gggtttggcc taggcttagc
     6181 gattggacta gcaggagcgt tgaatgcttc tgaaacaata attggttgtt tgataggcgt
     6241 attgattgtg tattgtttcg tagttcttgt cccgtctctt tctctcagcg ttcgacgttt
     6301 acgagacgca ggttttcatt gggcatttat ttttttctat tttgtcccaa cgattggctc
     6361 tcttgtgtta ctggttcttt attgcttacc gacaaaagag tcaatagaag ataaacaggt
     6421 acaggaggaa accagatgat tatagtaaca ggaattgttt tattagggat aggcgtttta
     6481 tttggctatc atttcgccta ttttccaaag attggcgaat ggcaacaaag acaagcgatc
     6541 caaaaatatt ttcaagtcaa aggcaacgaa tatacctatt atgctgaaga tgaggatagc
     6601 tatatcgtca gtttgaacaa tcaagaatat cgggtgaaat tttctttaaa taaccctatt
     6661 caagtggtgt atgcggtaga agtggtggaa gaagtgtttg actaatgatg atgattcgat
     6721 ttacagaagt tgataacatg tctttactaa cagataaaaa aattgcatta gataaggcat
     6781 ttattcaatt gaaaagagaa aatgaagagc tattggcacg agcggaaatt ggctggtgta
     6841 ccttggaaat ctacacagaa aaccatgaaa ttccagtatt tgaaggctcg tatgattttc
     6901 cagtagaaga gtttcatttg atttcattga ttgaacaatc tttaacagaa aatgggcaat
     6961 taagtgaaga agcacaaacc tttattcaaa ccttaaattc cgaattaccg aagttttatc
     7021 aagtaaagaa agaccaaaag aacgaacgtc gaccaacaaa aatcaaaaag gaaaagaaaa
     7081 caccactttc tgtagaaaca gcgtttacac aaagcaacgg ctctcttcag aagaaaaaaa
     7141 tgtcttttca atggcttttg tttggattta tcgtcgttgt atcttgtggt ctttttatta
     7201 tgcaactttc aacgcaaaga gagattgaac aactaacgaa aaaggagcac cagttacaag
     7261 aacgcattgc atttgcggaa gaacttgcga caaaacaacc acaaattgat acatttagtc
     7321 gttattttat tacgaattat tattcgggtg aaatggcaga agaacggtat aggaaaaagc
     7381 ttcaatcgtt tctaacggaa aaagttgcga aacaaatcag tcctcaaaag gaaaaagtca
     7441 aaagtatttt atcctgggaa atgaagaaag aaggcaagca ttggcaaatt gcgtatgtca
     7501 ttacattgga aaccgagaaa aagacacaag aaacaaaaaa agtgtctttt caattggaag
     7561 aacaggaaaa aaattatcta gtttccacgc ttcccgaact agatgatttt gaaataaatc
     7621 aataaataag agaagaggaa aaacacatga aaaaaatgat tttaggaagt ttatttagtg
     7681 caactgtatt gctaggtatg gtaggaacca gtgcgtatgc agatactacg tcaccaggtg
     7741 atccaagtgt accaccgaca gaaattattc caccaacgga agaaacacct gaagttcctg
     7801 aagaacctgt tttaccacca acagatggag gagaagaaac cccagaaatt cccgaaatac
     7861 cagaaactcc tgaaacccca gagattccac caacggaaga aaatccagat acaggtggag
     7921 gcacagaaac gcctgaagta ccagaaaatc cagatactgg cggaggggaa ggaacacctg
     7981 aagttccacc cgttgaggaa aacccaggaa atggtggtaa tacagagaca ccagaaaagc
     8041 cgtcgaatcc aggaggcaat gaaggaaata cgactcctcc aacgacacca cctaccaata
     8101 atggggggac gaacactacg cctccagtac aggacgtcgt agtaaaacca gatggaagta
     8161 ttggcagcca acaaacaggt ggacaaaatg ttcaaattga aaccaataat gcgtctgaat
     8221 taacccatat tccaacacca agtacaccaa ttgaaacagc ttcaggagaa aaaattgtct
     8281 ccgtcgttga tggtgtcgct tataaagaat caggaaatgg cttgacgccc atttcagaca
     8341 aggtcgaaca acttccaagc ggaaatattg cggtgaaagg gtctgatgga aaaatgaaag
     8401 tattgcctaa gactggcgaa gaagtgtctc tcgttgcgac agcacttggt ggattattgg
     8461 cttcaggaag cggttttgct ttttggcgca aaagaaaagc aacacaaaaa atgaactaag
     8521 cgaatgaagg atcaaacatg aagaaaaaac ttcttttatg gtgtgggttc ggttgtctac
     8581 ccacactatt actatgtatg tttggttttc tgtttatgat acttttgatt agtagtggtt
     8641 ctgaagagga agaaatcgac tgtagtccaa ccgaaattgt cgcaagtact atctcattaa
     8701 cgaaccaagg catggagaca aacgggcttc aaattgcaaa ggaagtacaa aaacatattc
     8761 caaatacgac attagagggt ctagcgggta tgttaggcaa ttttcaacaa gagtcgagta
     8821 tgagtcccac agcgattgaa cgaccaaacg atccattatc tgggcatggg attgcacaat
     8881 ggacggcagg gcgaacgact gctttgaaaa attttgccaa agagcgaaac aaagagtggt
     8941 cagatttagg tgtacaagtt gagtttttga ttcatgaact aaacacaaca gaaaaagctt
     9001 cacaagttgc attacaagca aaaagcgtag aggaagcaac agaacagtgg caagtgaaat
     9061 ttgagcgtgc agggatacca atgatgggga atcgtattac ttttgcgaat caatggtacg
     9121 caaaactagg aaagaatgac agtgatcccg tcgcttcaat tacattagaa aatggggcaa
     9181 acgcaggaag taaagacttg accgattatt gtctagaaac aacaggaaca agcagttctt
     9241 ccgatagtaa tgtattaaac gtagcaaaaa gtctgattgg ttattttcat tattcacaac
     9301 cgcaacgggc acaatttggt agtgtcgaaa atccagataa aaatggctat gccgattgtt
     9361 cgagctttgt gtggcttgtt ttaacgaaag cagggtataa aacgcctgcg aacgttgggt
     9421 ggtttaccgg ctcaatgacg agcgacgcaa gaggaagtaa gcaatactta caagaaatac
     9481 ctgagagcga ggcaaaagct ggcgatatta tcattgtgaa cttaggttct ggtgtgggaa
     9541 atgatggaca cacagctgtt ttagcggaag attggcatgg actttctacg aaagtaatcg
     9601 aacaaggggg aatgaatact aacggtgtag gtgaagggca agtcaattta tcctttgggt
     9661 atttgcttaa tggtggtgat atttgttttg ctcgaccgat aaagaaataa gaggtgtcaa
     9721 atgaagaaac gatttattta tgtttgtgta ggcggattgt tcttatttgg tgtaggcagt
     9781 acattttttc ttattcatga aaagctacag ctaacagcaa aggaagagaa actgacggtg
     9841 gagataaaca agttccaaga agagaagaaa ggacaggaag accttgtaac gtcactcaaa
     9901 gaacaacttc aacaagtaga aagcgaggtc acgacgttaa aaaaagcaaa tgaagaattg
     9961 aaaaacaatg attcaacaga aaaaggaaac attcaaacct ttcaaaaaac agtgacagaa
    10021 gcgttcgagg ttctgtttaa ttatgagcct gattcttttc aagaacgaaa aaacaattgt
    10081 gcaccatatc tttctgacaa actgatgaag caatatttca aagaaggcgt ttcctatggt
    10141 gattcaaacg gggtgtcttc tgaattagag aaatgttctg tttatttaaa aagcacacaa
    10201 aaagcgacta tccaagggct cgttgtggtg tcgtatcgca ataaaattgg tgatagttcc
    10261 tggaataatg ccatgaatat ttttgacgta acctttgatc cagcgtcaaa gaaaatcacg
    10321 tttatgcaaa atttgggaag tagctttaat caacagcttt tagaagaata agaaaaggag
    10381 aaggttatgg agagacaaga atcaataaga gaggtacaag aagtaccaac agaacaagca
    10441 aaagaaaccc aagaaggacg ttctttttct tatggaaaga atttttcttt tttacgcccg
    10501 tcgattcaaa tcagtgtgtt cgttttgtgg cggatacaca ataatcggta ttcggtaggc
    10561 agtcaactat tttatcaaga aatttgtcat gctttatcaa aaaaatacag tcaaaatgcc
    10621 tacaaagaag cacttgcctt tttg
//
LOCUS       DAZUKZ010000088        10634 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000088 DAZUKZ010000000
VERSION     DAZUKZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.090"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..328)
                     /locus_tag="ACG8EF_002133"
     CDS             complement(<1..328)
                     /locus_tag="ACG8EF_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4947425.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSI"
     gene            complement(457..1104)
                     /locus_tag="ACG8EF_002134"
     CDS             complement(457..1104)
                     /locus_tag="ACG8EF_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS4947426.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(1188..2615)
                     /gene="uxaC"
                     /locus_tag="ACG8EF_002135"
     CDS             complement(1188..2615)
                     /gene="uxaC"
                     /locus_tag="ACG8EF_002135"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS4947427.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(2689..3768)
                     /gene="uxuA"
                     /locus_tag="ACG8EF_002136"
     CDS             complement(2689..3768)
                     /gene="uxuA"
                     /locus_tag="ACG8EF_002136"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4947428.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(3771..5402)
                     /locus_tag="ACG8EF_002137"
     CDS             complement(3771..5402)
                     /locus_tag="ACG8EF_002137"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS4947429.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            5719..6390
                     /locus_tag="ACG8EF_002138"
     CDS             5719..6390
                     /locus_tag="ACG8EF_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4947430.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            complement(7226..8680)
                     /locus_tag="ACG8EF_002139"
     CDS             complement(7226..8680)
                     /locus_tag="ACG8EF_002139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947431.1"
                     /translation="MFDESLHLFPEYPDTVGSQAIPEKKQAVCQILLKEEVQLKQELT
                     AVILMDRICKEVLAGSDGTLAYGVVPLFDEVVRLLGNTQQIGAICGEFLLLPKMLVTV
                     ELAELATGMNEHPYMSGYFYLTVDDKNRQALMQAVTRLLTEKKLNILVGTVSLLGEGW
                     DCPAINLLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVPLSEQPDLK
                     RMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSCERSRLKEQ
                     WSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKGNLLEYFQI
                     RRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLENAGYREGQ
                     LFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQSSLPKCQLV
                     YTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(8681..8980)
                     /locus_tag="ACG8EF_002140"
     CDS             complement(8681..8980)
                     /locus_tag="ACG8EF_002140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947432.1"
                     /translation="MESQETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHN
                     YELIPEHWQLLGSSKQQGKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(9283..9654)
                     /locus_tag="ACG8EF_002141"
     CDS             complement(9283..9654)
                     /locus_tag="ACG8EF_002141"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS4947433.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
     gene            complement(9988..10251)
                     /locus_tag="ACG8EF_002142"
     CDS             complement(9988..10251)
                     /locus_tag="ACG8EF_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947434.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
BASE COUNT         3320 a         2154 c         1533 g         3627 t
ORIGIN      
        1 taatactcat agaagctgat gccattgatg tttgatacat taaaaaactt gctaaagcga
       61 ctggaccact catccgtaaa atccacggtt taaatttgcc agttttagat ggttttgatc
      121 catccacaat acgccccatt gttatgtctg taactgcatc aataaatctt gctactaaaa
      181 ataatgttcc tacggtagct ccgcttatcc ccaatacttt tgtataaaaa atcattaaaa
      241 aagaactagc aaaaatgaaa gtgaaatcat tcccaaaatc tccaaacata tatcctattt
      301 tgtctcttag accaaatttt ctttccatat aatttgcgct ccttcctatt gttaagcgtt
      361 ttcattctgt tgaactgcca acttacgtac tatactctga agtataaaga gttagtattt
      421 attttttaca ttttatagta ttatttattt tttctcctaa taattcttta tctcataaaa
      481 cttatctata tatttactag ctattcgagt tacttcttca aaatcacctt tttcaacagg
      541 agccaacaaa ttcccaccta caccaactgc aacaacgcct gcttcaaacc actcttctaa
      601 gttatctaga cttactccgc cagtaggcat tatatttaca tatggtaatg gagcttgata
      661 cgtttttaca acagatggac caaaagcaga acctggaaat agtttaatga tttctactcc
      721 agcggtaagt gcctccttta tctcagtaat tgtcatacaa cctggtaaat aaggaatttg
      781 atataaatta caaatctctg ctgtttgtcg atcaaaagat ggacttacga tatattctgc
      841 tccagccata attgcaatac gagcagtaaa cgcatctaaa actgtaccgg ctccaattat
      901 tacatctttt tgatcagcat attttttagc aatttctttg attatttctt cagcattcgg
      961 aactgtaaaa gttagttcaa tccctttaat tccacccgca gtaattgcat ttattgcttt
     1021 aatggcctca tctttatttt ttccacgtac aaccgcaatg actccagccg cttctaactt
     1081 acttaaaatt tctacttttt tcatatctat ctccttcttt tatggataca tactctagct
     1141 agttgtacta acttacaata aataaattta agcttttatt ttttgtatta ttttctactt
     1201 tcgatttcaa aaaaattaaa ataattatac gcattacgat aacaaatatt ctccactatc
     1261 tgactaagat acatttcatc atctggaatt cgacctgctt taacccactc gcttagtaat
     1321 tgacataata ctcgacggaa atactcatgg cgaggatagc ttagaaaact tcgagaatca
     1381 gtcaacatac ctgtaaaatt cgacaacaaa ctttgttgcg ccatcgtagt caactgttga
     1441 cgcattcctt cagctgtatc attgaaccac caagcacatc ccaattggat tttttgaatc
     1501 ataccccctt ggaagctttg cattgctgtt gctagtggaa gccaatcggt tggatttaat
     1561 gaatataaaa ttgtttttgg taagcaccca ctcttttcag cagtgtcgaa caatgctaca
     1621 acattttgtg tcaaactagc ttgatccccc atcgaatcac caccgacatc tactccaatt
     1681 tgttccttta atatacttga attatttctt aaagcattta aatgcatttg catcgtccaa
     1741 ccatatcttg tatactcaga catcaaatgt aattgaatac ctgtaacaaa ttgtgcaatt
     1801 tctgataaat ggaaatccgt ttttcctgct aaagctttta ttaacagatt gtcgagtatt
     1861 ttttcatctg ctatttcata aaaataagta ttcaaaccgt gatctgctaa tcgacagcca
     1921 atttgatgaa aataatccac tcgttggctc aatgcctgct tcaacccatt aaagtttttg
     1981 attggtatac ccgctgcttg actaagtttt aaaatatagt tcgaaaatcc tgatgatttg
     2041 atactccaga ccttatctgg acggaaagta ggtaatacct tgaaagaatt atcctgttta
     2101 gaaagctcag tatgatatcg caagtcatct gtaggatcat ccgttgtaca aatggctttc
     2161 acatccatca tcttcagcaa ttcttttggg cgataggaat ccgtttggat tttttcgttg
     2221 gcacgtcgcc aaatttctgg tgcattcttc tcagaaattg tcaaatcaat atcaaagtaa
     2281 cgttgtaatt ccaaatgaga ccattcaaaa ataggattac ctggcgcctt ctccactgtt
     2341 tttacaaaag ctaaaaattt ttcataatca ttacctgcac cactgatcaa ctcttcttct
     2401 atcccattag ctcgcatcaa tcgccattta taatgatctc cagctccttt atcatttaac
     2461 catacctgag tcaaattctt gtattgctta ttttctagca aacatgctgg gtctaaatga
     2521 caatgataat cgatgattgg ttggtgttct gcaaatttat gaaaaagtct tttagctatt
     2581 tcattctcta ataaaaagtt cttatctaaa aacatattta agcactcctt tccttccctt
     2641 agtttttaga agagaaaatt tttcttctaa atagactcca ttatataatt atttactcgc
     2701 ccccttttca agcgcttccc aaataccatt taaataaact gcacctagag ctcggtcata
     2761 taacccataa ccaggtctac cggtttctcc ccaaatcatc cgaccatgat ctggacgaat
     2821 atagccatta aatccattat cgtacattgt ttttaatatc ttatacatat ctagagaacc
     2881 atattctgat ttatgcggcg cttcataaaa atcgagtcca ttatcactta aatatttgat
     2941 attacgaaca tgtgcaaaag gaatccgatc ccgttgacaa aattcagata gaatttcata
     3001 gacatcatta tctggtcgtt ctgcaatact tccagtacat aacgtaatac cattagaagg
     3061 agaatccact acattacaca accattctaa atcgtcacga tttttaacaa tacgaggtaa
     3121 tccgaaaata gaatatggtg gatcatctgg atgaactgcc attttaatcc caacttcttc
     3181 acatgtcggg ataattgctt tgagaaaata tgcaaagttc tctcgcaatt tcgcttcatc
     3241 aacttcttta taggcttcta ataattgctt gatttgtgat aaccgatgag gttcccaacc
     3301 cggcaattca aaattattag tagaattttc tacttttcta ataatttcct ctggatcagt
     3361 aggaatattt tttgaaatat aggctaacgt agaagaccca tcatctaaat gataatccaa
     3421 atctgatttt acccaatcaa agactggcat aaaattataa cagataacct caatcccaaa
     3481 ttctgccaaa ttgcgaatcg tctgtttata attctcgatg tattgatctc gtgttggcaa
     3541 accaattttg atatcatcat gaacattaac actctcaata actttcaagg taaggccatt
     3601 ggcttcgact tcatctttta aagctttaat tcgttctctc ggccagacct ctccggctgg
     3661 tatatccatt aatgttccaa caattccttt cattgctggg atctgacgaa tttcttgtag
     3721 tgagattttt tcttcatttg ccccatacca tctaaaagtc atttccatag ttactttcct
     3781 ccaacttctg ttatatattt attgattgtt ttacgaactg cttgaggacc tactagcatt
     3841 tcgcaaaaga ttttttcaat tttctttcct atctcaactt gatataaatc taaaccaaat
     3901 atttccttat tttctaagat ttcctgtacc gcacaatgaa tctttgcagg atcttgttca
     3961 cctaatttca atactgagaa tttttcctgc aactctacca ataaaggatc aggacttggc
     4021 acaaaatttt ttccttgatc atcgactgcc aataaataac gtaaccaagc agcaatagcg
     4081 ataggaataa atgttagatt ttctgcattt ccgtcttttt cgacatactt tttaattgtt
     4141 tcaccatagc gaatcgctac tttttgagaa gtatctgttg caattcgttg cggtgtatct
     4201 ggaatatagg gattaggcaa acgtttattg attacttcat caataaattg ttttggatca
     4261 ataacgcctg gactttcaac tactggtaag ccttctacat aacctatccc tttaattaag
     4321 ccaaccaaat catcatcttg catttctgcc tgaattgatt tataccccaa caaacaacct
     4381 gttattgcta atgccgtatg gagcggattt aaacatgtcg taaccttcat gatatctgct
     4441 ttatcaactg tttctctatc tgtcataatc acaccagccg cttccagttt aggtcgacca
     4501 tttggaaaac tatcttcaat gactagataa tggacttttt cagtattggt aaaagcagcg
     4561 atattggtat ttttcgcagt atgaattata tctaagtctt cgaaacccgc cttcaataaa
     4621 tccattttga cactttccga aggatttggc gtaattctat caatcatact ccaaggaaaa
     4681 gtcacttttt ctttatcctt taaataagca ataaattttt cagaaacgta tccttttttt
     4741 tgccaagctt ctgcaatcat aaaaatgcta tcttgaaaac gttgaccatt ctgtgagaaa
     4801 ttatcagtag aaaccatcgc aatcggtaac tctccagcat taaacctgct atacaacaag
     4861 gatgtcacaa tactcatcgt atgttgagga ttggtaggtc cagattttat atctgcttcc
     4921 actaccgaaa aaaattgtcc tttagaatct tttaaactgt atcctttttc agtaatagta
     4981 aacgttgtaa actgtaatgt aggatcttca aaatatttaa ccactctttg atagtccgtt
     5041 atattctttg gattgcaata taaactatca gctgttactg ctaaaagtct ttttttgagt
     5101 tcaccatctt cacccataac aatctccaaa atatcattgt tgtagtcatg ataagctttt
     5161 tctactactt cttcatcaaa tgtctcacac acaactactc cggattctaa ttcattttga
     5221 tctgctaagc attgagcaat ttctgcatga aatcctcgat aaagatttcc tccaccaaaa
     5281 tgaagccaca ctggatgttt ctgaccttct tgtttgaccg cttcgacatt aaaatacggt
     5341 agttgaactt gcattttgtt caatccttcg atagaatctt gccagtcttt aatcaaaacc
     5401 atatttaccc ctccatactg atatgttagt ttccgttaca attgtagtat aaaatcgaat
     5461 gtaatcgctg tcaataagaa aaaactaaaa atattctttt ttctaccatg tcagaattat
     5521 tgtgtctttt tttagtaaaa gcacaccaat atatattttt tagaactaac ttaaattaaa
     5581 gcttaatttc tctttgatct tatttgagta ttttttgttt ttttaatagc tagtatgtta
     5641 ctttgaaatt tgatattata taacgagcat agaactaaaa tatcaaattc aaaaatgaaa
     5701 ttttgagagg gattaaatat gacaattaaa actagtctcc aaaatcaagc ttatcaatct
     5761 attcgtcagc aaattatcta cgctgactta gaacctggaa aaaaagtatc agaaaaaaaa
     5821 ttggaagaaa cgctttctat cggtcgtact ccaatacgtg aagcattgat tcaactccgt
     5881 catcaaggac ttgttgatac aattcctcaa agtggaaatt atatctcatt aatcgactta
     5941 gatttagcaa aaaatgctcg ttttgtcaga gaacatttag aacgacaaat tatgctagag
     6001 tgttgtgcca agatgaataa tcaaaagaaa caagttttag aaactataat tgcagaacaa
     6061 gaaaaagctg ttagtcaaaa gaatgctcgt agtttttttc ataatgataa tttatttcat
     6121 aaagcttgtt ttgaaattgc agaacgggaa gaagtatggc aatggttgga agataataat
     6181 acgcacttgg aacgatttcg ttggttacga gtaaaaacgg aaggattaaa atgggaaaaa
     6241 attatggaac aacatcatca actatttaaa gccttgacca tgcaagaccc tgaagaagct
     6301 aacttcttaa catccttgca tcttcattta atgcttgatg aacaaaatgt tgtattgact
     6361 actcacccaa aatattttaa aaatatataa ctgctaaatt tctttttttt aatttattta
     6421 catttctgat tactacagaa aactaactac tttttttatg taaagttctg tcaaatatag
     6481 tcactttata ttactttctc ttgagtaaaa aaacatgtga ggtcgtaaac aaagattgaa
     6541 ctatctacat tcatactcta taatctgttt atttgcagac tgataatagc aaaagaaaac
     6601 ttataatcaa ataatatcaa catttgatca cataatttga gattgaatgc tgatttacct
     6661 atgttttacg taatggtctt tagttatttt tgctcataaa atgcataaaa cacgcgtttt
     6721 tatgtgtgct tgaaatttct tggctctata atttctaaga ctgataaaaa tattctatcg
     6781 gttctttttg caaataaaaa aacatataga agctccatta gattgaatca ccacaacaca
     6841 atcaaaggag cattccgtat gcttgatttt cattctataa aactactttt aggaatcaaa
     6901 gaaaatcaca acggttcaaa ctaaaagcaa gaatatccga agtaccattg tttcatcgat
     6961 catgagaaaa gcattcacct ctgtctgcag atgttcttat atcgctctgt cagtcctatt
     7021 aataaagtac tgttttgttt tcaaaaaaac tattacgagt actttttaag agaatgcctt
     7081 gcaaatattc atcacacacc gttatataag tcaaatagtt tctttcagtc gtcaagaaaa
     7141 gattctcctg tccttggcat tatcagttaa gtagcaactt aattctcgga cttgttctac
     7201 tctagcaaaa tttattttca tcaacttacg tccaaatgtt tttttgaata attacttcat
     7261 cgcgggtctg gaagacttgc ctcattcgat aagtcactag cttttggcgt ccttcaggat
     7321 ttctagtata caccaactga catttgggca gcgacgattg atttaattcg tttagaaaaa
     7381 ggttagcgat ttgcttattt cggccaaagc gttctggtac cgtcagaact atctttttcc
     7441 cttcgattaa ataccgcgga ttctccagcg gtgctaaaag ctccctcata gcttcagtaa
     7501 aaagctgtcc ttctcgatag ccagcattct caagatagca tctaaattcc tgctgatcag
     7561 ccgctacgat tacttttcta tcactaactt caccaaggcg ctgcaacgtc gtttgaacgc
     7621 atatggccaa gctttttttg cgggcacatt ccttttccca tagcttttgt cgcctacgaa
     7681 tttggaaata ttccagcaag ttacctttaa atatagctga ccagaagctt agaggctcta
     7741 tattttttac tactatcggc aatgaagttt cttgtttttg ctttacctga tcgactttcc
     7801 taacgacgat tggcatagat aggtgccagc catcagctaa agcagtactc cattgttctt
     7861 tcaaccgact tcgttcacaa gaaaaataga acatcgactg agtataagtt tccatcgtgg
     7921 tggtcgtata ttccagtggt aaagcaaagc gatcgccacc actctcaatt tttggtgaag
     7981 attcaaaggt cagacctgaa atattgccca aacgccgtag cattcttttc aaatcaggct
     8041 gttcagacag aggaactccc ggtaatccaa tcgctaaatg ccaaatgttc gttactttat
     8101 tttgtgcacc taggcgtaag ccccgccctc gtagttgttg tgtttgcaca aaagtggaag
     8161 cttgattagc caagatcaac agattaattg caggacaatc ccagccttct cctagcaaac
     8221 taacggttcc taccaaaata ttcaacttct tttcggttaa taggcgagta actgcttgca
     8281 tcaatgcttg gcggtttttg tcatccaccg tcaaataaaa ataacccgac atataaggat
     8341 gttcattcat acccgtcgct agttctgcca gctcaacagt tactagcatt ttcggtaaca
     8401 gtaagaactc tccacagatg gcaccgattt gctgagtgtt acctaataga cgaacaactt
     8461 catcaaacaa aggaaccacg ccataagcta aggtaccatc actgcctgct aatacctcct
     8521 tgcaaatacg atccatcaaa atgaccgctg tcagttcttg cttcagctga acttcttctt
     8581 ttaacaaaat ttgacaaact gcttgctttt tctcaggaat cgcctgacta ccaacagtgt
     8641 ctggatactc tggaaacaaa tgcagcgact catcaaacaa tcatccagct tttttcaatt
     8701 gataaaaaat aggcaattcc ggttgatctt caaacatctg ctgataaata gtgatcagct
     8761 cttttttacc taaagatgga actgaagctt ttttaccttg ctgcttagaa ctgcctaata
     8821 gctgccaatg ttcgggtatc aattcatagt tatgatgatt caagtaaatt aatgcactag
     8881 tgaacagctc aaaattttca taaatttcct tcgaatgctc aagcggatgc tgaacaaatt
     8941 ctaaagaaag caaataatcc accgtctctt ggctttccat taacgcttcc accagctcat
     9001 tttgtttctc taaaaaggta aaataatgct gagtagtttc ctgattagcc ggaatcaaat
     9061 atagaaaatc ctgatgagga catagaacct cctcctttac caactcggga atgctaattt
     9121 cttcgtcaat caggccattt taactcaata tattgcttcc actccgtctg agagctatca
     9181 atcggaggcg tggctgtcaa ggaaatcgtc aacagattcg gttgtttttt cttcaagtca
     9241 atcaacaatt caccccagct tttttttaaa tgatgagctt catcaaaaaa ctaacagctc
     9301 cagctgatca ccataagttg gctcctcctt cagtcggttg taaatggtct gatacgtttc
     9361 cactgtaata tgactaggat gttcaatcgc cccagaaaga tagcaactat ctgctggatt
     9421 tactaagaaa tattcctcga ttgttgttaa ccactgtttc ttcaatatta gggaaggaac
     9481 caaaattaag gttttacaat ccaaacgtcg gatcatctca attcctaaaa aggtcttgcc
     9541 agcccctggt ggagccacta gatggactga attgttattt aaataagtag caaagttagt
     9601 caatatacgc ttctgatagc tacgccactg aattttttca tcagatagcc acatcccagg
     9661 tccccctctc acgtattttc tctatcataa tctaaatgaa tacagcaaaa aaaagatttc
     9721 ttcaagaata tgtgttttta tagtaatatt ttacttactc taaactcaaa tgaaaaggta
     9781 aaaaaactgg tagtacaatc ttttaaattc tcattgtttt gtacgttctt tactatcaaa
     9841 aaaacaaaaa aaacgcgttt ctacgcgtga ccaaaatttt tagctctata atttctaaga
     9901 ctgatagaaa atcattctgt ttttgaagtc ttttttcata aatactactt gatttattgt
     9961 gtatattgta taaaagattg ttcataatta ttttttataa ttaaaatttg gtattgatga
    10021 ccagcgattc cgtatataat gcgcaatcaa attaagcttt ctgtctctgg taaatattcc
    10081 tgttctgttt tcttgtactc gtaagatttc ttcacttatc gcaaaattca taaaattcca
    10141 cactttttca taaacaaaat gtacacattt atctattact tcataatagt ctacttgata
    10201 ttcttctgta aacatggttg ctactgtatc tgcaccatat ttgataaaca tttgggctac
    10261 tcccttaaat tgagaaaagc ccattagttt atctactaat acttctaaaa tttcttgctt
    10321 tcttctttaa catcgtcccg taaggaatca tgtagtatgc tggaggaagt tcattttcta
    10381 aataattgta tcgcttcaat tgctcaaagg ctattctcta caattaatta caccaattac
    10441 tttatcttca cttatataag atttctaaac atttttcgtc aaatgaagag aaaatatttt
    10501 tttaaatgat cttgctcaag atttgtcaac gaatctttga attcgttttg aagttctgag
    10561 gaaggtatca tttaaaaact caatctgact ttcatcattt cgtttactcc cattttgatc
    10621 accttctacc aaca
//
LOCUS       DAZUKZ010000089        10532 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000089 DAZUKZ010000000
VERSION     DAZUKZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.091"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..162)
                     /locus_tag="ACG8EF_002143"
     CDS             complement(25..162)
                     /locus_tag="ACG8EF_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS4947435.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /gene="mobT"
                     /locus_tag="ACG8EF_002144"
     CDS             complement(159..1343)
                     /gene="mobT"
                     /locus_tag="ACG8EF_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS4947436.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="ACG8EF_002145"
     CDS             complement(1599..1853)
                     /locus_tag="ACG8EF_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947437.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="ACG8EF_002146"
     CDS             complement(1878..3020)
                     /locus_tag="ACG8EF_002146"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFS4947438.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="ACG8EF_002147"
     CDS             complement(3080..4432)
                     /locus_tag="ACG8EF_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS4947439.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="ACG8EF_002148"
     CDS             complement(4503..4847)
                     /locus_tag="ACG8EF_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS4947440.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="ACG8EF_002149"
     CDS             complement(4857..5231)
                     /locus_tag="ACG8EF_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4947441.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="ACG8EF_002150"
     CDS             complement(5244..5558)
                     /locus_tag="ACG8EF_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4947442.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ACG8EF_002151"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ACG8EF_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="ACG8EF_002152"
     CDS             complement(8976..9638)
                     /locus_tag="ACG8EF_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947443.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="ACG8EF_002153"
     CDS             10255..10485
                     /locus_tag="ACG8EF_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947444.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT         2895 a         2344 c         1786 g         3507 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ct
//
LOCUS       DAZUKZ010000090        10193 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000090 DAZUKZ010000000
VERSION     DAZUKZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.092"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..626
                     /locus_tag="ACG8EF_002154"
     CDS             120..626
                     /locus_tag="ACG8EF_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS4947445.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="ACG8EF_002155"
     CDS             643..1542
                     /locus_tag="ACG8EF_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFS4947446.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="ACG8EF_002156"
     CDS             1629..2513
                     /locus_tag="ACG8EF_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS4947447.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2705..2776)
                     /locus_tag="ACG8EF_002157"
     tRNA            complement(2705..2776)
                     /locus_tag="ACG8EF_002157"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2741..2743),aa:Arg,seq:ccg)
     gene            2836..4170
                     /gene="rpoN"
                     /locus_tag="ACG8EF_002158"
     CDS             2836..4170
                     /gene="rpoN"
                     /locus_tag="ACG8EF_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFS4947448.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4319..5410
                     /locus_tag="ACG8EF_002159"
     CDS             4319..5410
                     /locus_tag="ACG8EF_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFS4947449.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5451..6452
                     /gene="gap"
                     /locus_tag="ACG8EF_002160"
     CDS             5451..6452
                     /gene="gap"
                     /locus_tag="ACG8EF_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS4947450.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6562..7752
                     /gene="pgk"
                     /locus_tag="ACG8EF_002161"
     CDS             6562..7752
                     /gene="pgk"
                     /locus_tag="ACG8EF_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFS4947451.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7856..8611
                     /gene="tpiA"
                     /locus_tag="ACG8EF_002162"
     CDS             7856..8611
                     /gene="tpiA"
                     /locus_tag="ACG8EF_002162"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFS4947452.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8727..10025
                     /gene="eno"
                     /locus_tag="ACG8EF_002163"
     CDS             8727..10025
                     /gene="eno"
                     /locus_tag="ACG8EF_002163"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFS4947453.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2112 g         2630 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
     2641 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
     2701 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
     2761 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
     2821 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
     2881 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
     2941 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
     3001 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
     3061 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
     3121 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
     3181 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
     3241 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
     3301 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
     3361 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
     3421 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
     3481 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
     3541 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
     3601 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
     3661 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
     3721 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
     3781 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
     3841 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
     3901 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
     3961 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
     4021 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
     4081 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
     4141 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
     4201 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
     4261 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
     4321 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga
     4381 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
     4441 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
     4501 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
     4561 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
     4621 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
     4681 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
     4741 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
     4801 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
     4861 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
     4921 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
     4981 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
     5041 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
     5101 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
     5161 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
     5221 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
     5281 catggcaatc gcgggcggaa aatcaaaagc caaagcaatt cgagcttata tgaaaaatgc
     5341 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
     5401 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
     5461 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
     5521 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
     5581 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
     5641 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
     5701 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
     5761 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
     5821 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
     5881 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
     5941 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
     6001 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
     6061 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
     6121 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
     6181 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
     6241 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
     6301 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
     6361 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
     6421 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
     6481 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
     6541 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
     6601 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
     6661 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
     6721 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
     6781 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
     6841 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
     6901 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
     6961 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
     7021 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
     7081 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
     7141 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
     7201 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
     7261 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
     7321 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
     7381 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
     7441 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
     7501 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
     7561 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
     7621 gactcagccg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
     7681 ggtggcggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
     7741 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag
     7801 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
     7861 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
     7921 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
     7981 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
     8041 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
     8101 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
     8161 ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac
     8221 accaatccta tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
     8281 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat
     8341 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
     8401 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
     8461 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
     8521 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
     8581 attcttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
     8641 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
     8701 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
     8761 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
     8821 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
     8881 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
     8941 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
     9001 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
     9061 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
     9121 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
     9181 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
     9241 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
     9301 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
     9361 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
     9421 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
     9481 aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga
     9541 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag
     9601 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
     9661 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
     9721 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
     9781 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
     9841 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa
     9901 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
     9961 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    10021 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    10081 cttagttaag taatcgaact ctctacttgc tgaaatatcg gctctttgtc aataaggact
    10141 gatgagttgt gcaaaatcaa atctgagtca gaatgaacca cattctggct cag
//
LOCUS       DAZUKZ010000091        10101 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000091 DAZUKZ010000000
VERSION     DAZUKZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.093"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            149..796
                     /locus_tag="ACG8EF_002164"
     CDS             149..796
                     /locus_tag="ACG8EF_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS4947454.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    925..1141
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1189..3834
                     /locus_tag="ACG8EF_002165"
     CDS             1189..3834
                     /locus_tag="ACG8EF_002165"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFS4947455.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4144..5457
                     /locus_tag="ACG8EF_002166"
     CDS             4144..5457
                     /locus_tag="ACG8EF_002166"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFS4947456.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5447..6100
                     /locus_tag="ACG8EF_002167"
     CDS             5447..6100
                     /locus_tag="ACG8EF_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4947457.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6155..6706
                     /gene="radC"
                     /locus_tag="ACG8EF_002168"
                     /pseudo
     CDS             6155..6706
                     /gene="radC"
                     /locus_tag="ACG8EF_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6837..7922
                     /locus_tag="ACG8EF_002169"
     CDS             6837..7922
                     /locus_tag="ACG8EF_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4947458.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7919..9811
                     /locus_tag="ACG8EF_002170"
     CDS             7919..9811
                     /locus_tag="ACG8EF_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS4947459.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            9818..>10101
                     /locus_tag="ACG8EF_002171"
     CDS             9818..>10101
                     /locus_tag="ACG8EF_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFS4947460.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKT"
BASE COUNT         3487 a         1582 c         2145 g         2887 t
ORIGIN      
        1 acgtttggtt gagcttatta ccgcagatgc aagcttttga acaccgtttt atttgtctca
       61 cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt acactaaata
      121 tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac tgccaaaagc
      181 aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg actcaggtat
      241 aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg caacgatccg
      301 cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg atgtcccttt
      361 tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa tcgcattagt
      421 aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa atgaaaatct
      481 aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa ttgatggaga
      541 aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg tgacagtagc
      601 aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg tcaaaggaaa
      661 tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt atgtcaatgt
      721 tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg agaattactc
      781 agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa ctcgtcgtaa
      841 ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac ttgttgacta
      901 aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa ctggcacatt
      961 cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt gaaggtagct
     1021 tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta actcacttga
     1081 gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat acgtcctaga
     1141 atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat gacggaagaa
     1201 aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta tcagcattgg
     1261 ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta ttcaatcgta
     1321 atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga tacgaccttg
     1381 caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg gctgcctggt
     1441 atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag agagcaaggg
     1501 atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga atggaaagaa
     1561 gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt agattacagt
     1621 agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt ttttgttacc
     1681 ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga tcctcaagca
     1741 cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc cttttatcat
     1801 atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac ccgtccagaa
     1861 acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta tcaagcgctc
     1921 atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat tgcagatgaa
     1981 tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca tgatccaaac
     2041 gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga tgatgatggt
     2101 acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc tcgtaaagca
     2161 atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat gatccacagt
     2221 gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac tcaatggttt
     2281 gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga tcaagcagtg
     2341 gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa tgtccatgat
     2401 tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta tcacaaagaa
     2461 acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg ggtacaagat
     2521 cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac aatgggctgg
     2581 ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt agtaaccggt
     2641 tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga atttactggc
     2701 gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca agggcggaag
     2761 atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa gtatggagca
     2821 gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt ccgcttcagc
     2881 tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc ttcacgtttc
     2941 gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg agaaaaatca
     3001 gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt aactgagcta
     3061 tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat ctgggatgat
     3121 ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga tgctgtttca
     3181 aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg tttattgcat
     3241 ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca gggaaattct
     3301 ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac agcttctaaa
     3361 gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc agaagtgaat
     3421 acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa aatcgatcaa
     3481 tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga gttgacgata
     3541 tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg tgcagaaatc
     3601 ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt agaaaaagaa
     3661 ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa tgaacgtttt
     3721 gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa agattattta
     3781 gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca ataagaatag
     3841 aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga acatcgttac
     3901 aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga cataaatttt
     3961 gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg tggatcatgt
     4021 atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt ttctctttgt
     4081 catactttta tttttcttat gtgttctatt tgttataata aaagagaaat gggggaattg
     4141 ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt tggttcacgt
     4201 cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga gagaaaagta
     4261 aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta tttgagaagc
     4321 ctattagaag aggctgggct gacagtaggt acatttactt caccttatat cgaaacgttc
     4381 aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat ccagcttgta
     4441 aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc tggtattact
     4501 gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa agtggacgta
     4561 gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc tgtaccaatg
     4621 ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga tacaattgaa
     4681 gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt aacaggaaat
     4741 attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa tgcaccgctt
     4801 tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat gtggggagaa
     4861 gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc gcttttaggg
     4921 caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta ctgtcaattg
     4981 gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc tcgatggcca
     5041 ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc ccataatacg
     5101 catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata tcaaatcaac
     5161 attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg attgcagcaa
     5221 gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat cgaattaagt
     5281 gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca attcggatta
     5341 gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg ttcactttat
     5401 ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga agtataatgc
     5461 agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt acgaagccac
     5521 acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt taaaattctt
     5581 aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg aatttggcaa
     5641 aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac gtttttcgca
     5701 aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag aagaacatca
     5761 gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt tattgaagaa
     5821 gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc aacgagcaaa
     5881 accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga agaaagaaac
     5941 attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg agatcccggt
     6001 agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa cagctggggt
     6061 tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct cttttcgtat
     6121 cttattttag ataagataga aaggagatgt ttttttggga aaacgattga tcaaagaagt
     6181 acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag ctttatcgga
     6241 tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg tgatgtcgat
     6301 tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg ctactttgca
     6361 tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca aagccttgat
     6421 cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg ttcgtaccag
     6481 ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag aacatttggt
     6541 ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt tcatcggttc
     6601 gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac gttattgtgc
     6661 agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttttaacca tagtataata
     6721 aataatagta gttaaagtag tttaagagga gatattaaag ggatatttta ctttaaataa
     6781 aaattaacaa ggtttataat ttgtagtagt taaagtattt aaagaggtgg gaacatgtga
     6841 aggttcaaag gatagaagtg gagaataagc cgtatccatt gtatttatta ctagataaag
     6901 aataccagct aatagaacca gtaatgaaat ttattaaata cttagataat actggtaagt
     6961 ctcctaatac cattaaagca tactgctatc atttaaagtt gctgtacgag ttcatggaac
     7021 agagaggtgt tattcttaat gatattaact ttgagttgtt agcagacttc gtaggttggt
     7081 tgagatatcc ttcagcatca aatgtaattg atcttcagtc aaaaaaagcc ataagagaag
     7141 aaacgacagt gaatacaatt ttaaatgtag ttatgagttt tcttgattat ttaagtagat
     7201 taggagaatt taaatcaatt gatgtattta aacaagcaaa gggaagaaat ttcaaaggat
     7261 ttttacatca tgttaataag ggtagatacc aaaagaatgt cttaaagtta agggttaaaa
     7321 agaaacagat aagaacattg agatcagagg aagttaagca aattattgat gcttgtcata
     7381 cgaaaagaga taaattaatt ttaatgctta tgtatgaggg tggtttaaga atcggtgaag
     7441 tgttatcgct taggcttgaa gatattgtca cttgggacaa tcaaatccat ttaacaccta
     7501 gagatgttaa tgttaatgaa gcttatatta aattaaggaa ggaaagaaca atacatgtga
     7561 gtaaagaact tatgtcactt tatacagatt acttgatata tgagtatagt gaggaattgg
     7621 agcatgatta tgtttttatt tccttaaaag aaggctattt tgggaaacca ctaaagtacc
     7681 aaagtgttct tgatctagtt agaagaatag ttaaaaggac tggaatagaa tttacatcac
     7741 atatgcttcg ccacactcac gcaacgcagc taattaggga aggatgggat gttgcgttcg
     7801 ttcaaaagag attaggtcac gcacatgttc agacaacgtt aaatacctat gttcatcttt
     7861 cagatcagga tatgaaaaat gagtttaata aatacctcga gagaaaggag cataagaaat
     7921 gaatgcttct agtaaaagga aaattattag tcagagtgag attagcaaaa aaatagctgt
     7981 aatgaatgaa gaaatgcagg ggttttgggc taataatagt tgggatataa gaaaatgtcc
     8041 acatccttct gccatagaat taagtaagaa tcctgcttta aggaatcgtt gggttcgttt
     8101 tgaacgtgtt aaaaatctgt ggttaagaac agaattgaaa tatttttatt tttaccattt
     8161 aaacaatgga atatggaatg caaaaactgt ctggattaga aaaggaacag taattaataa
     8221 aatgttagat ttcttagatt taaagtatcc tagcattact tcaattactg aagttcctat
     8281 tgaaaaagca atgacggagt atagaactta tttgacaaaa cggggtgtta gaattaccac
     8341 tactaattat aagattactg ctaatcaaga aaaaacacct gtaaaagcta attcctacta
     8401 tgttactaat ctaaaacaat ttatggagtt ttatgagaac ttttattttg atggagagga
     8461 gtgggataaa gacgtttggg atagacgtaa cttacctttg ccagatgata aggttaaccc
     8521 aacacaatat gaatatacaa ttaactttaa agggtttcgg aatacatatt ttaaacaact
     8581 tgtaaaaaga tattgtaagt tgagattgaa catggatagc ttttcctatg taagtgatat
     8641 tgcccaaaga cttaaagagt tctttaattt tctggacatg aaatttaaac aagttcagag
     8701 agtacaccaa ttaacgagag tggaaattga agcatattta agtgaactaa acatgatggg
     8761 aataaaacct agtacaataa ctgggaggat ctctatattg gaaggactat ttagtaccct
     8821 tcttaggcta gaatgggatg atgttccttc caaaatatta atttattctg aggactatcc
     8881 gaaaatacca agagcaaaac cacgctttat agatgaattc gtcctagagc aattgaacag
     8941 tcatcttgat aaattacccg aatatatagc tacgatgact atgattgttc aagaatgtgg
     9001 aatgaggata agtgaattgt gcaccttgaa aaaaggctgt ctattagagg acaaagatgg
     9061 agatttcttt ttaaagtatt atcaatggaa aatgaaaaag gagcatatag ttccaatatc
     9121 taaagaggta gctttactta ttaaagttcg ggaagataaa gtttcagagg aatttccaga
     9181 tagtgaatac ctctttccaa gaaaagatgg atcgccatta aaacaagaaa catttagagg
     9241 tgagttaaat aaattagctt atgagcaaaa tatagtggat aaatcaggtg agatttatag
     9301 attccatgcc catgcctttc gccatacagt aggaacaaga atgattaaca acgggatgcc
     9361 ccagcatatt gtgcagaaat ttttggggca tgaaagccca gaaatgacaa gcagatacgc
     9421 tcatatcttt gatgaaactc taaaaaatga atttactaaa tttcaggaaa aactggttac
     9481 caataatgga gatgtgcttg atctagatga agataatgaa gtcgatgatg tagagcttca
     9541 atggttcaag aaaaatataa atgcacaagt gcttccaaat ggttattgta gattgccagt
     9601 agtagcaggt ggttgtccac atgcgaatgc atgcttagat tgcactcact tctgtaccag
     9661 taagcaattc ttaccacagc acgaagaaca gttagagcgt acagaagagt tattagccat
     9721 agctaaggat aaacaatggc aaagacaagt agagactaat agccgtgtta aagagcgttt
     9781 agaacaaatc attggaagtt tgacggggta attatcaatg gataaacaag ttagaaatac
     9841 aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa aagtagacaa
     9901 agcgatttct aaattttcga ttgaaggtaa agttattaat tttaattcaa tagcaaagga
     9961 agctaatgtt tctaaatcat ggctttataa ggaacacgat attaggcaaa gaatcgaatc
    10021 ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa cccaagaaaa gttctcgttc
    10081 ggaggaaatc cttattaaaa c
//
LOCUS       DAZUKZ010000092        10086 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000092 DAZUKZ010000000
VERSION     DAZUKZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.094"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(247..759)
                     /locus_tag="ACG8EF_002172"
     CDS             complement(247..759)
                     /locus_tag="ACG8EF_002172"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFS4947461.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ACG8EF_002173"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ACG8EF_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFS4947462.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="ACG8EF_002174"
     CDS             complement(3212..3655)
                     /locus_tag="ACG8EF_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFS4947463.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="ACG8EF_002175"
     CDS             complement(3656..4444)
                     /locus_tag="ACG8EF_002175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4947464.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /gene="rnz"
                     /locus_tag="ACG8EF_002176"
     CDS             complement(4441..5382)
                     /gene="rnz"
                     /locus_tag="ACG8EF_002176"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFS4947465.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="ACG8EF_002177"
     CDS             complement(5559..6146)
                     /locus_tag="ACG8EF_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFS4947466.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="ACG8EF_002178"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="ACG8EF_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFS4947467.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="ACG8EF_002179"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="ACG8EF_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS4947468.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="ACG8EF_002180"
     CDS             complement(9063..9998)
                     /locus_tag="ACG8EF_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4947469.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DAZUKZ010000093        10054 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000093 DAZUKZ010000000
VERSION     DAZUKZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.095"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..226)
                     /locus_tag="ACG8EF_002181"
     CDS             complement(29..226)
                     /locus_tag="ACG8EF_002181"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS4947470.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(372..1250)
                     /locus_tag="ACG8EF_002182"
     CDS             complement(372..1250)
                     /locus_tag="ACG8EF_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4947471.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1289..2407)
                     /locus_tag="ACG8EF_002183"
     CDS             complement(1289..2407)
                     /locus_tag="ACG8EF_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS4947472.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2404..4503)
                     /locus_tag="ACG8EF_002184"
     CDS             complement(2404..4503)
                     /locus_tag="ACG8EF_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFS4947473.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4554..5375)
                     /locus_tag="ACG8EF_002185"
     CDS             complement(4554..5375)
                     /locus_tag="ACG8EF_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4947474.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5372..6181)
                     /locus_tag="ACG8EF_002186"
     CDS             complement(5372..6181)
                     /locus_tag="ACG8EF_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4947475.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6202..6696)
                     /locus_tag="ACG8EF_002187"
     CDS             complement(6202..6696)
                     /locus_tag="ACG8EF_002187"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4947476.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6708..7208)
                     /locus_tag="ACG8EF_002188"
     CDS             complement(6708..7208)
                     /locus_tag="ACG8EF_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4947477.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7273..9969)
                     /locus_tag="ACG8EF_002189"
     CDS             complement(7273..9969)
                     /locus_tag="ACG8EF_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4947478.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         3056 a         2264 c         1655 g         3079 t
ORIGIN      
        1 atttatttta tacggctgaa aaaatctatc accaaaaaaa ttcaaacatt aagccaagga
       61 ttatgaagtc aacatttcga aaaatgaaca tacattggta agcaactata actattacca
      121 cagacataaa gtaacacaat atattactat ttaagtgtac gccaccagtg aatatcctca
      181 aggtatacag cgcacaacaa aaagaaaaaa ggaaacgcag aagcatttcc tttcagtgat
      241 ttaatgaaaa caacacgatt tgacttagag cctttttatt tgaattcatt ttcgctttat
      301 ccaacttcat ttgatcattt gattactcgg ttaaatactt ttgacatgag tatcaatgaa
      361 acattcatta attatggttg cttatttctt accgcttttc tggccaatgc taatccgccg
      421 attattccgg cattatcacc taattcgggc gtaataatgt agctatcaat tggtggtatt
      481 tcaacatatc catttataag ctcaacaaat ttatcctgaa cttttttctt catgtgttct
      541 tgtttcatta caccaccgcc aaaaataatt ctatctggcg aaaacatcag tgttgtattg
      601 tatgcacatt gggcaaggta aaaggcttca atctgccaaa acgaatcgtc agccaataaa
      661 ttctggcctt ttacacccaa ccgtttttca atcgctggtc ctgctgccat cccctctaaa
      721 caatcttggt ggaagggaca gtttcctgaa aaattgtcct ttggatgtcg cttgatcaac
      781 atatgcccca tttccggatg actgaaccct tccacgaaag tcccattgaa caaggcacca
      841 cctccgatac ctgttcccac agtgaagtat gcaacacctg ctaacccttt gccagaacca
      901 gcaacatatt caccatagca agccgcattg acatctgttg tccattcaat cggaatattg
      961 aaatattgct ttagcgttcc tacaaaattg aaattttgcc aagcaagttt tggtgttgat
     1021 gtgatatgac cgaaactcgt tgatttgcat tgaatatcaa ttggaccaaa gcagccgacc
     1081 ccgatgcctg ccaattgttc cttaaaaggt ttaaaaaaat caatgacctg cttcattgtt
     1141 tcaactggag ttgttgtggg aaaactgact ctttccaaaa tcgttaattc ctcagtaccg
     1201 actgcacaga caaatttagt ccctccagct tcaattgccc catatatcat cataggttcc
     1261 tcccttcgaa tatgtgaaaa acacttcatt aacgcggcaa gcgttctcga tcagtgaaat
     1321 gacgtttagt atttcctgaa gcttctgttg aataccagta agcgacacta gcaacatcgt
     1381 cttgccgctc aaataagcga acatcatcgt tgccgatttg ttgtaaagtg accgataaat
     1441 cttcggtaaa acagatgggg tcttgaagat gccaacgata aagaccatgt cgtggcattg
     1501 cgtcgtcacc aaaggcatga acggcattca attttcccgt ttcaaaacga tctcttgtgg
     1561 catctctatt tgattggtaa ggatacccta aaaaggctgt tgaatagttt ttcgcatgtg
     1621 gacgtccgaa ctcatcgtac tcatgaaagg cccacgcccc gccaaaataa tcttctgaac
     1681 ctgttgaggt tacggtcgga tactcactat ccccatcaat gtagaattta aactcgcctt
     1741 cgccccacca ataacgttct aggcaggtaa gcgcaaggta agtacccacg taatagcctt
     1801 gaccctttaa tcgatcaatt aacttataat cctcctgtaa tttggtaatg cgttcccgat
     1861 gccaatttgc gtgaaaatat aggctatttt cttccaattc gtccaccaaa gcgtaattta
     1921 tcgtataaaa aaatgaggac atatccttcg gatgtaaatt ctcgatcgta attttagcgg
     1981 atgtttggaa aggcatttcg aaataggaat taaagccccc tgtaggatta accactatcg
     2041 gcaacgagtt cacctcgcaa cgctctccaa acccattaca aaagaaatcc cctaaaggac
     2101 tttcaacagc agggctatcc gacccatccc aataaattct taagattaaa tcacgtaaaa
     2161 caaaactgcc aaattctgtt cgatcacgaa tcgtcatcca catatgacga attattcccg
     2221 ttccagtaat atccgcaatc acagtttctt caccagaagt cagcggaatt gcgccacgac
     2281 ctttgcggct tggtcctagc tcgcttggca acatcgcagc tttacctttc tcgcctgttg
     2341 gattttcggg agtgattgct cgactctgga tttttttaaa ttgggtaata ttttttagta
     2401 tactcatctt tttctccttt gaatgtgaat cgtttttccc ttttctacgt gaagaatttt
     2461 tttcgtatca cttattttca atggcttttc tgacaaattt gaaatactca actcggattg
     2521 cgtacattca atcgaaatga aggcttgacc gaacttcaaa ttcttccaag aaaacgttaa
     2581 attatcaaca ggatcaattt gcaatactcc ttttttgaat ttcaatccga aatattgact
     2641 gatcattagg ctgacaaaaa gacccgatgc ccagccgcat ttgccgcagc caaagtctcg
     2701 cacgacttct ccttgttgag cctctaagcg atacggccac caccaccacg aaccgtctaa
     2761 atctgccaaa caaattagtt cttctaatcg ggttacgaaa gcttgagggt tgtgcagctc
     2821 gctcattaaa acagtgataa agccaggaaa agtggcgccg gattctaatc cccaagtgat
     2881 gcctttgatt tcctcgctat aggttggatt gaaagaacta gcagaaaagg ccatggtgtt
     2941 ctgatataaa tgatctttat tatctaaaaa ttgataaaaa ggcatcaacg tagtgtctga
     3001 ttcttctcca tcatgcatca ataaattaat ttttccatcc acaatcacat cacttaaaaa
     3061 gattaaccct tgctcaagaa tcggtttttg atagtgcttc actgaatagg ttgttttctc
     3121 ctcatcaccg attccctcca aaaattgctc accaaaagga ccatcgatag tcatttgttt
     3181 acaaattgat tcctttagtt tacaggcaat gtttttgtat cgcttgctaa gactcagttg
     3241 ttctagggct tcaaaaattt ctgcaagtcc aatacaggcc ttccaaagac aaagattgct
     3301 gccggtatga tactttccaa gtgcaaaggc gtctgataac caaacagatg gaaaaagcat
     3361 tggttcccct gaccgacgtt gagctaatat gtcctcaata attcttgaag cattcagtag
     3421 aacctgcgga caccgctcaa agaattttgt atccccagta tgttcaaaat aaagactagc
     3481 cagtaacgca ctggataacg aattactcag agaatgattg atacctcctg taaattttga
     3541 accaggaaac ttaacgccat attgatcaaa ccatagaacc gttttttgac ataactccgg
     3601 ttcaaacatg gtgaatggca atgatgcgta atacatatcc ttattccaaa ttcgattagt
     3661 agcaggatag ctgccccaat tgctgccgac gacctgtccc tgtcgattca taccgaaact
     3721 agaaaagctt tgataaagtg cacgacgata gagatggaca actctatcgt ttggcattga
     3781 cagctgccca aatagctgag caaaatagtt caatgtttcc ttcagccatt tttcgatatc
     3841 agattcctga aaagtcatta cttcttcaaa agcatttggg tccacaaaag caatggcgaa
     3901 gcttgccact tcctgttgtg cgatcgtgtc cttatttcct ttttgagaaa tcaacacatt
     3961 ttgctgatcc gaatacttct cttgatacga aggcacatct ttcactgaaa cttgaaggct
     4021 ttcgtgtgtt tcattcacaa tgtaaacctg ttgaacaagc atacttaaac gcttattagc
     4081 cagtatggga gcaaaggata ctacaatgac cttaaacgtc gaataatgat gaatatatct
     4141 aggcaaacag tcttgaatca catcgacttc aatacgatga tccgattctg acaaatggta
     4201 gacaatcccg tttacttcaa cctttaagga taacgaattg gtttgagaga gctgtttatt
     4261 gacccaaaca cctggttttt cctcagttag taaattttct cgataaaagg tcaacgtttg
     4321 aatcgtcccg caaacatcaa ggtttgctaa tgtcttttga ttacttacat cgaaggtcaa
     4381 tccctttaca tcaggaatat tccgatagta gctgtattcg tttgaagtag tcacatttcc
     4441 gtcgtttaac tcagcattaa aaaatgggtg tttgggtgaa taaattactt cattaatagt
     4501 catcttttcc ctccttgcta aaaaaactgc agcctagtag ctgcagcttc ctactatgcg
     4561 attccaagta atgacaataa aattcccaag acgaccattc caaaaataat tttgatgacg
     4621 ccgacttttt tatctagtaa tttgaaacag ctcaatgtta ccaataacgg tacaattcct
     4681 ttaaaaatac tatcgaggat ttcttggaca ttcattagtt cttcgccctt cattgaaaag
     4741 ctcaaaacgg tattgaattt gaccatactg atcgtcattg cacctaccat gatcagaccc
     4801 aagatactag cagcttttgt cagaatttca attaacccgt tcgcatacag cttttcaata
     4861 tatttagagc ctaatgaata gcctaaaacg ccgccatagt atcgcatcaa gtaattaggt
     4921 atattgaaca taactaaaaa gataattggt gccaaaatat ttccggcatt ggctaagttg
     4981 atcgctagtc ccgcggtaac gacacgccaa actccccaga acaatgaatc gccaattcca
     5041 gccaatggtc ccatcaaact cgttttgatc gcgttgatac tttccacgtc aaaaccagct
     5101 ttttcagaat tttccttctc catcgatgcc gcaatcccca tgacaaagga cgcgcaagga
     5161 atggtcgtcg aaaaatactg tgtatgacgt tccagcgccg cagctcgatc ttcttcactt
     5221 ttgtagaaat gattaatgaa gggcatcata gaataggcga aaccaccgcc gcccatcgtc
     5281 accggattca ccgaagcata catagtcatc gagcgccaaa acatcttccg aaccaatttt
     5341 ttctcatctt tagaaattcc atcagctaat ttcattgaaa gaaatcctcc tcttcttggt
     5401 caagcgttgc atttacagca gcatcgcttt gaactgcatg aacagatata ttttttagtt
     5461 gtagatctct ttgggctata acaattgcaa taatcgttcc aactgcggca attgcaatca
     5521 atggtaactc gagatacgcc ataacaacgg ccccaaagaa aaaccacaca gcaatatttt
     5581 tactccaaag catccgtaac agcatcgcca tcccaactgc cggcagcaga tttccagcga
     5641 ctgttaatcc gttcatgatg acctctggaa tattatccaa gacgcttttg acaacatctg
     5701 ctccaaataa aacggcaaag aatggaataa gagcataaag cgcatagtgg acaaaccaca
     5761 taaagaagtg cagattgatg attccttttt gatcaccaga ttttgcaaat ttgaaaaatt
     5821 tagcagccat cggtccacat acaaaagaaa aaatcatatt cattaactgt aatcctaata
     5881 ctgctagtgg aaagacaatg ggaataatcg catccacgtt tcctcccatt ttgattgcga
     5941 acgctgtagc cagcccagca gccaaagaag gctccgccgt actagagatt ccgatattga
     6001 cgacacccat aaagatggct tctagtgacg ccccgacaat caaccccgtc tgcaaatccc
     6061 ccatcaacgc tcctgctaac ggagcaacaa caatcggacg actcagcatg gacatcccga
     6121 acatttcatg tccagcaatt ccaataaaaa cggccaaagc gactacaaat gcatcataca
     6181 taatttttct ccttcctttt tctaaaaatc actcaaaata gagatagctg aacgtttttc
     6241 catcgccggt gtaggctgaa aaacggtatc tggataaagt tcaactaatt cttttaagtc
     6301 ttgaatttct gacgtcgtta acatcacttc ctttgataag gttttcttat cttccgatgg
     6361 cggtgtcatt ttcccagcat tccccacgtt cattaattcg atttccgttg ggaaagcttt
     6421 cgcaactttt accgcatctt tgacattagg gactaaaacc atcaagcgca tgtccttcgc
     6481 ccgcggatct tttaacaaat gaatcgcgtc atcaactgtt ttaataatga ctttgacatt
     6541 cgatggtgca gccatcttca atgtcatctt ttgaatatcg ttggtagccg cctcatcgct
     6601 tgctactact aacccggtta aatttagact ctttgtccaa gtcatcgcta cttgtccatg
     6661 aattaatcgt tcatcaattc gcactgcttt aatcattttt cttcctccta aaaaaaatct
     6721 tcctcttcat ttactacatg tacctcatca agaatggtca tctttaattc ctgttggcat
     6781 ttatttacca gttcctgaat atcctccttt gttaattcct tggttgccaa tactatttcg
     6841 agcagaattg gcagattaaa gccagacaaa atagttacag caatctcttt ctccaaaaag
     6901 gttttggtaa tcttctgatt gacacttccg ccaaataaat cggtaaaaac aaaaatctga
     6961 tcctgctcgt tcacctggtt tacaaataca ttcaactcct gttcaaaatc acactcatcc
     7021 acataagcat taatcaccgt aatctcttga accttccccg tcagtaattg aatactgcta
     7081 agaaagcctg aagctaagtg accgtgagtc gctattagaa ttttcttcat atggttaatt
     7141 cctttctttc tgttcacttt tatataagca attcctgtgc caacttttaa aaacattggt
     7201 ttaatcacgt taattcacat taaaaaaagt gtctaaccta aaaaaaacag attagacact
     7261 tttttagaca ctttattttt gaatatacgg tcctaataat tcatagagca gcgaaagctc
     7321 ataagcattg actttaaaat tatatttcat ctccattgat tgaaaaatac ttctagaaac
     7381 ataataaaat tcatcttcat ctggtttcaa atcataattc ggttcttcag atttttctgt
     7441 ccgagccaag aataaacgtt ccatcatcaa tgccgtatgc atatagagat ttagttttac
     7501 acggccattt aaagtgaaat gatagtaatt ttcatattta gaaatgactg tttccacttc
     7561 actcatcacg atcgttgggt tcagaaagtt caaacggtcc gcaattcctt ccaacgaaaa
     7621 gaattttact agctcctgaa tcaagcggtt aaattctgcc ggcttcattt caggactcaa
     7681 aatatgctcc aaattggctg ctccatcacc gtctaagatg tcataaacat taatattagg
     7741 aattgaaaag gaacgtggaa gatccgaagt cgtgatgacc aattttgtct tgtcaaaata
     7801 agtttcatca ttttggtcga ttaattcacg tagttctctg tagtccttcg tgaaaacttc
     7861 gatggattca tccgataaat gatgacgaaa aacatctttc aatttgtccg aaatccccaa
     7921 acccgacata caggaaatga taatgttgct actttgagaa aagccttcaa agtattgaac
     7981 attgattgaa tacttgtctg ctgcatcctc cgcaatctgt ttaaatggcg cattcatcgt
     8041 catcttcaag ccaatatcaa gtgcaatgga agtcgtcaaa ttattcagaa gaagtaactc
     8101 cccttttaaa tcgttcttga tctgtgtgta aatttgattt aaagagccca tatctacgat
     8161 cagcactaga ctatctgtat tcacttgccg tttcacgaaa ttccttgtcg attcgacaat
     8221 ctctgacaca ttactctcta ctggcatatc caacgcttca aaaacataat tgccgcataa
     8281 ttgattcact actgcttgaa tgctgctagc cgtactatct ccgtgagcaa gcaacaggcc
     8341 cttcagctcg atggattcat ctacataatc agataaagtc acgacaataa gcagtcttag
     8401 aaagttctcg tccttttctg agagcggtgg taaatgctcc aagaactgat tacataagta
     8461 agtcgtccga ggtagatttg cagctaatgt acgatttatc ccggttaagt ctgtcttata
     8521 gcttggggaa atgacatagg atctgctaat aatttctgca acaggtccac tcgtcgtcaa
     8581 tccgtacgtt tttcctaaaa cggataccca gacctttcta aacatctgaa tcatcaattt
     8641 tctactcatt gataaaaaag aatccttatc aatgtgatgc accaaatgct gtacttcatt
     8701 ctttatcgaa gaaaaatttt cattgaaatt accttcattt atcagctgag taattttttc
     8761 caaaatcgaa tcataatcaa acggtttgat tcgatccgtt tttatctttt gatttttctt
     8821 tatcaataaa ctagcaatcg gcaagctttc tacttcggat tccagcatat ttaactgaat
     8881 caagatctca tttttcccaa tcgttaatgg ttcttcttgt gttcgactat acacatccgc
     8941 acaactgatt ttaatgatat tcttcagctt accaatattc ccactaaagg gatgagataa
     9001 cattaaagac aaggcttctc ttgaaacaat aatatctttt tgtaaaacat ccgcttcatt
     9061 ttgaaaaaat aattgaatca gctctaatcg ttctcgaatg gggcgatctt caaaagttgg
     9121 caacataaca ctaatttgaa tacgccgatc aaaggtgtct aataaataat tttcaccttt
     9181 ttccgtcgtg gcgaaaataa aacgaacctt agcagaatgc caatttacat tttcaccgac
     9241 tggacgatag tttccagtat caataaatag aaagagcttt tcttgatttt ctttcgatag
     9301 gcgatggacc tcatcgagaa aaagatagcc gttgttggcc gttgccaata aaccatcggt
     9361 atccttttca gcccccgtaa aggccccctt cttgtatcca aacagtgttg ccgaaagcag
     9421 ttcgggattg tccgcataat cggcacagtt taagatagca aatggtgcat ctttttcaat
     9481 aatttgctca tggatagcgt attcaaaaat ttttgtggct aaaaaacttt ttccaacacc
     9541 cgtggctcca tttattaata cactgagtcc gttaggcgga tatttgacag cggctataca
     9601 tttttgaatg acctctctca gacttccgtc atgaccaact acagaatgaa aggcttcgtt
     9661 tatttgctcc gtattatcta caaccgcata ttcatctaaa ggcttccaca aaacaggctt
     9721 tcccggtaat ttctcgacct ttttatcttc cactaatctc gtcaaataat ggctggcatt
     9781 ctgacgagag atacctagat tcttcgaaat tttctcggca gtgacagaag acatattccc
     9841 tgttagtcgt atcagctcat caaaaactcg atctttagcg ctctttttca ttttttcctc
     9901 cagtttaatg tgtctaaaag cataataaac tatcggctgc aatatgtcta ttctatattt
     9961 gtattccaat ttttactttg aaacacaaat agtcgaatat ttgcctcaga tatctgacaa
    10021 aataatttat ctaccaattt agtttttatc taat
//
LOCUS       DAZUKZ010000094        10027 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000094 DAZUKZ010000000
VERSION     DAZUKZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.096"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(491..715)
                     /locus_tag="ACG8EF_002190"
     CDS             complement(491..715)
                     /locus_tag="ACG8EF_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947479.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            906..3683
                     /locus_tag="ACG8EF_002191"
     CDS             906..3683
                     /locus_tag="ACG8EF_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4947480.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3764..4183
                     /locus_tag="ACG8EF_002192"
     CDS             3764..4183
                     /locus_tag="ACG8EF_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4947481.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4195..4665
                     /locus_tag="ACG8EF_002193"
     CDS             4195..4665
                     /locus_tag="ACG8EF_002193"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4947482.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4682..5461
                     /locus_tag="ACG8EF_002194"
     CDS             4682..5461
                     /locus_tag="ACG8EF_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4947483.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5448..6269
                     /locus_tag="ACG8EF_002195"
     CDS             5448..6269
                     /locus_tag="ACG8EF_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4947484.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6286..7335
                     /locus_tag="ACG8EF_002196"
     CDS             6286..7335
                     /locus_tag="ACG8EF_002196"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4947485.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7348..8352
                     /locus_tag="ACG8EF_002197"
     CDS             7348..8352
                     /locus_tag="ACG8EF_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4947486.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8985..10004
                     /locus_tag="ACG8EF_002198"
     CDS             8985..10004
                     /locus_tag="ACG8EF_002198"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS4947487.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3362 a         1651 c         2094 g         2920 t
ORIGIN      
        1 ttttttaatt tgttcttgca atcttttttg ctacgtaatt tttgtaaaaa agtgggttga
       61 gagaaatcta attgattttc aaagaaggag tatgaaaatg agaaaaatat ttattcgttt
      121 attgtgggta ctatcttaga ggaataaata atagtggaga aaactatggt ttggcgcaga
      181 ttctatcagg tttgatggaa cgaagacaac ctcccgacat caagtcaaaa gctacttgag
      241 attttgatct gaaaataaaa aaagaaaaca aggtaggaac atcaatgttt ctaccttgtt
      301 ttcgtaatca atgggaaaaa actaagagtg gtgtacgtaa tcaataaaaa ggatggtgac
      361 aacagtacct ctttactctc tgaaagcatc ataactcgtt tttcctcatt atgccaataa
      421 attgttgtta taccacatat aaggatttgt aatagctaaa aacagacagg aaagttgaaa
      481 tctgtgaatc ttattttgta tggaaggatt tatagaacgc aaaaatcaag ataaagatga
      541 ataaaaatcc tgtttcggct atatattgcg tatctacgcc aataaaacga gtttgttctg
      601 tcatagccat ataaagcaaa ggcaaaaata caactacagc gagataagat ccgatgattt
      661 ctcgaaaagt tttccaggaa gtatgtttcc tgtttttata tttatccgat ttcattgaat
      721 agtcatagcc tccttattct ttcatatgtt gaaattccag tataaaaact ggaaggaact
      781 gtacgttttt tagaataaaa attataaggg ataagtaggc tgctgtctat aataagatat
      841 atttttcaat tctttattaa agattttatt gacaaaatga atccgttttc tatgatggag
      901 gaggggtgaa taagttgttg aagaaggaaa ttttatgtta cttagagaac cagacagcct
      961 ttttcgattt agaacatatg aacgaggtct ttacagcaca aaggttagct gatcattttg
     1021 gggtaaaaag aaacacagtc agtcattatt tgaaccagtt aaatgatgag caactactag
     1081 tcaaaatcaa ttctcgacca gtgatttatt tccacaaaga agcttttgaa aaacaatttt
     1141 ttaagttacg cacgaatttt tatttttcta tcgatgatct caaagaggaa cagccttttt
     1201 tcgcgcaacc aaaagactta ttctctttta tgatcggata cgacgccagt ttgaaagaaa
     1261 gtatcgaaca aatcaaaaca gctctttact atccagatgg cggattacca cttttgatta
     1321 caggcgaaag tggcacaggt aaaagttatt tagttaattt ggtctatcag tattgtctgc
     1381 ttcatgatct gctagaagat tctgcaccat tcatcacttt taattgtgca caatatgcag
     1441 ataatcctga acttttgact agcaatttgt ttggacatgt taagggagcg tacactggtg
     1501 cagaagaaaa taaaaaaggg gcttttgaag cagcagatgg tgggatcttg tttttagacg
     1561 aggtacatcg cttaacacca gaaggtcagg aaaaactgtt cacctatctt gatcaaggtg
     1621 tgatttatcg tatgggagaa cctaatttgt cacgccggat caaaacacgt ctcttttttg
     1681 ctacgactga agagattact agtcatttct taacaacttt cattcgaaga atacctatcc
     1741 gaatcgaact acctgcactt aatcagcgta gtcgtaatga acggctagaa ctgatttact
     1801 ctttttttct cgaagaacaa agaaaaatgg ggagcccttt gtcagtcagt ggaccagctt
     1861 tgtcactttt aacaagtcag aaattatccg gaaacatagg agagctgaaa aatatcgtca
     1921 aagtttcagc agcaaaagca tatgctgaac agcgtgaaca gcctgaaatc catgtcacga
     1981 tccatcattt gcaaaaagaa ctacttgcgc agcctattac taaaaataca gcacagcaag
     2041 gaatctatat tacgcaagac cagaccttag agcaattaat ggagaagcaa cagcccgaac
     2101 agcagcgaat cgttaaaagt tttgagcaaa tcctaattga tttcaagaaa aataactgtc
     2161 tgctatattc ttgcgaagga aaattaaagc aagaagtgat tcaattattt gattttttgc
     2221 tgtttgagac cagtagacaa gaaaaacacg agctgctggt ttattggaca caatctatcc
     2281 gagagacctt tcgacaaatg gaaactgcct atcaaatcaa attcgatgga aacagtgtct
     2341 atgccttgag ttactatctc tatcagcgaa gcactatcaa atggttaccg gaagaacatg
     2401 atattatcca actaatcaaa gaattagaac aacaagtttc tcagtcttat ccatctagtt
     2461 atcactatgt ccaacgaatt ctggaattaa gtaagatcaa attagatatt gaagacacaa
     2521 gtatggatcg gattttactg acgatctatc taaaaaaagc gaaatggtca aaagaaaaac
     2581 gactgccaaa agcgatgatt gctgctcacg gttatgcaac tgctagcagt atggcaaatg
     2641 tagtaaatcg tttattacaa gaggatctat ttgaatcatt tgatatgcca ctagatgtca
     2701 caccacagca gattgcttca gaaatccttg attattgcga aaacagcgat gtttcaaatg
     2761 gattgatcat attagtagac atgggatctc tgaaagaaat ccatcagttt tttaaaaaac
     2821 agttatccgt accgcttttg attttgaata atgtgacgac acctttagca atcacagtag
     2881 gggaatgcct gcaaaaaaat gtatcattag aagagatagc agaagagact gttcgacaga
     2941 tccagccaga atggcgactg ctctatccag aggaaaacaa acccaaagca ctcattacta
     3001 cttgcttcac tggaatcggt acggcggtcc acttaagtga actgctagaa aaaagcttac
     3061 ctacggcttg tcagttgaaa atcatcccat atgaatatca gcaattaaaa gataagaaaa
     3121 acagtgaccc attattttcc atatacgaag tcgtaggaat gattggcacc acagatcctg
     3181 atatcaccaa tatcgactat ctttcattag aagacctcat ctcgggggaa aagatgtctg
     3241 tactagctga atggttggac actactatga atgcttcaga gaaggaaata ttcaatcagc
     3301 gcatcattcg taatttttca ttggaaaaag tactggattc cgtgactatt ctagatacag
     3361 agaaggtgat gggagaaatc gatcatttta tgagagaatt agaggtgcag ttgaatcgaa
     3421 gcatcagcaa tcccaaaaaa ttagcgttgt atgtccatgt gagctgtctc attgaacgat
     3481 tgatccgaca aatgccaatc gaaacctatc aaggattaga gaaatggaag cagtgtcaaa
     3541 aagaagggtt atctgctatt aaatctgcgt ttagtgtcat tgaagagcat tatagtgtca
     3601 tgatccccaa ttctgagatt gcctatatct atgatatttt gagtgaagat acagagtttc
     3661 tctcgataga agaagaattt tgaacataag caagttgttg ccactaattt tggcatgcaa
     3721 cttgcttttt tagtatgtgt aagtgaaaag aggagatgaa aaagtggata gagaaattat
     3781 cttagcttcc catggaaaat ttgcttcagg gatattagat tcattagagc tgatctatgg
     3841 gaaaaatcat tctattactg tattcgattg ctatactgat gagaattttg atttagcgga
     3901 aacagtaagg cagcttttta tgaggtacaa agataaagaa atcattgttc tgacggatct
     3961 ttttggcgga agtgtcaaca acgaattctt acaatatatc aatcgggatc atgtctatct
     4021 cgttgccgga ttgaatttgc cactattgat tgagctagta tctcggttgg acgctgaagt
     4081 agagacaact gttttgatcc agcaggtgct aactgattca aaaaaaatga tccagttttg
     4141 taacgagcgg ctcaatcaga aaatagaaga agacgaattt taggaggaag aaaaatgatt
     4201 gtattgacaa gagtcgacca tcggttatta cacggacaag tagctttttc atggacacaa
     4261 acaataggcg cagactgtat tttgattgct aatgatgatg tacctacgaa tgagatacga
     4321 aagacaacga tcaaattagc caagccacaa ggagtcaaac tagtgatcaa atcaattgat
     4381 gactcgattg ccgcgttgaa aagcggggtg acagataaat ataagttatt catcgtagtg
     4441 gaatcgatcg aggatgcata taagctggca gaagcgtatc cagaaatcaa aaccatcaac
     4501 ttaggaggga tcaaggcaaa acctggtact cgcagcatcg gaaaagcggt aaatatttta
     4561 ccagaagagg aggaacgctt gaaacgatta attgacaaac aaatagaagt agaaatccgt
     4621 caagtaccag cagataaaaa agtggatgta agagaagttt tgtaagaaag gaagaacctc
     4681 aatgattgta caagcagtct tattaggaat tgttgcattt gtcgcacaat cagaatatgc
     4741 actagggaca tcgttgcttt ctcgtccgat tgtgacgggg ctgtttacag gaatcgtgtt
     4801 aggtgatgta aaaactggga tcattatggg cgcaacttta gagttagcat ttatcggatc
     4861 gttctcagta ggtgcttcga ttccacctga tgttgtgacc ggtgggattt tagggacggc
     4921 ttttgctatt acagctggag ccggtacaga aaccgcgctt ttactaggtt tgccgattgc
     4981 tacgttgaca ttgatattga aaaatattta cttagggctt ttgattccaa ttatgaacca
     5041 taaagcagac aattatgcag aagaaggaaa ttataaagga gtcgagcata tgcacttgct
     5101 tgccggtatc ggtctttcct tgatgcttgg tgttgtggta atggtttctt atatggtggg
     5161 aagcaatacg atcggaaatc tattaaatat gattcctgac ttcgttcaaa aggggttgtc
     5221 agtagctaca gggttgattc cagctcttgg ttttgcgatg cttgctcgtt tgctgatcaa
     5281 caaacaagtt gcaccatatt ttttcttagg ttttgctata gcttcttact tagagattcc
     5341 tgtgaccgga gtagctattt ttggtgcgat tttagcagtt gtggtagtca atattatgaa
     5401 tgtccgtcaa ctaccacttc aaacaactgc aggggaggta gaagaagatg aagaattctg
     5461 agatcaaaaa cagcaaaata acgaaaaaag agctaaatca cgtattttgg cgttcttttc
     5521 aaatggagtt ttcttggaat tatgaacgtc agatgaatat ggcttatgtt tatgcaatga
     5581 ttccgatttt gaaaaagtta tatcagtcga aagaagaaat ggcagcagct ttgaaacggc
     5641 atctagagtt ttttaacacg accccacata tcgttacact gattttagga atcacggctg
     5701 caatggagga agaaaataag gatgatcctg aatttgatgt gactgcgatc gatagtatca
     5761 aaacatcttt gatgggacct cttgcagggt taggagattc tttcttttgg ggaactttgc
     5821 gattgattgc cacaggtgtg ggtacctctt tagccatgca aggaaatatt ttaggcccta
     5881 tcttgttcat tttgattttc aatattccgc acattctgtt tcgctatcta gcaacaggtt
     5941 ggggatatcg cttaggaacg ggttttttga aaaagatcca ggcaaacggt atgatggaaa
     6001 gtttgacatt aggcgcgtct attattggac tgatggtcgt tggagcaatg actgccacga
     6061 tgatcaccat caatattcct ttaaaaatcg gttcaggaga aaatgctgtg actgttcaaa
     6121 gtatttttga tgatatcgtt ccaaatatct tatgtcttgg agcatttggc atagtgtttt
     6181 atctattgaa gaaagaagtg aaaccattaa cgatcttgat cggactagct ttctttggaa
     6241 tttttggttc gtggataggg attttttaaa aaaggagcag agaaaatgtt tacgatgcaa
     6301 gattatatct atgaagaaaa ggaagtacta agtacgatct tgaaaaagaa tgatttttcg
     6361 acaagagaac atatgaaaaa aacagtgaat ttactgatct tagcaacggg ttcatcttat
     6421 aacgcttgtt tagcagcgaa gccagcattg gaatcttatg gtgatcttac tgtcgatatt
     6481 caagaacctt tttatttttt caattatgga aaactttctc cttcaatcga tactgtgatt
     6541 gctgtgtcac aaagcgggaa aagtgcttct actgttgaag cagtaaaaat gatacaaaaa
     6601 caagggttac ctgttgttgc tatcacaaat gacgtgcaaa gtccacttgc tttagaagct
     6661 gcacaaatta ttgatttagg tgccggggta gaatcagtag gcttcgttac aaaaggctat
     6721 tctgcgactg ttttacagtt actactctta gggataggaa ttggaataag taaaaataaa
     6781 atcagcaaaa aaatagaaca agactacatg caacagttgc gcaaaataat caaccattta
     6841 ccagcgatta ttcaaaaaac ggaagaattt tttgatggat atcaaagttt atttcgttta
     6901 gcacagcgtt tcgtcaccat tggatatggt ccaaactggg gaacagctaa agaagcagag
     6961 acgaaattga ctgaaacaat ccgcgttccg tcacaaggat ttgagttaga agcttatatg
     7021 cacggcccat acctggaggc agatgcttcg catttgctat tctttattga aggggaaagt
     7081 gtaaataagg aacgttcgca aaagttgcaa agatatatga gtcggtacgt tggagagact
     7141 ctcaccatta ccacaaaaaa agcaagaaac gagaaaacac ttggattagc aatagaatgt
     7201 gatgaatatc tgtctgtatt agcgcttgtc gttccgtttc aactattcgc ttataaaacc
     7261 gcagtagcta aaggtattga cttgaacaaa aagatttttg aagactttga tacagtcttg
     7321 aaaagtaaat tataagaggt ggaaatcatg ctgaaattca atgaagaaga acaaatcaaa
     7381 gcaataaaag gcgcactagc tttacgtccg caagtagaag aaatcatcga taaactttat
     7441 gcggaaaaat ttgacgctgt ctattatcta ggaatcggtg gaacgtatgc ttcctcgatg
     7501 caagctgtca cttatatgaa tgggaaaagc aacttgcctg tctttgtaca gcatgctgca
     7561 gaatattaca ctactggaaa caaaagatta acaaaagatt ctatcgttat tttgtcctct
     7621 gttaccggta caactcaaga agtagtgaaa gcagtagaac aaatcaagaa agtaggagcg
     7681 acactgattg gatttatcga taaagcgaac agtaagttgt ctcagctatg cgattttgtg
     7741 gtcacttatc cagctcctgg aacagaacaa atcaaatttt ttatggcagc agatcgattg
     7801 atgtatttga atggagaatt cgaagcttat tcagaatact atgaacagct cgaacgttat
     7861 ttaccaacag ggttagtgga agcagagaaa aaagcagatg catttggctt atcatttgca
     7921 gaaaaacatc gacgtgatag tatgcattat tttattggag cagggaatca atggggagct
     7981 gtttattcat atgccatgtg ttactgggaa gaacaaagct ggcttccatc gaaatctatc
     8041 catgctgccg agtttcttca cgggacgtta gagatcgtag aagaaacaac cccagtcaca
     8101 ttatttttag gcgaagatga acagagagtg ttatctgaac gtgtggctaa acttttgcca
     8161 aaaatttgca gcaattatac attgatagac acaaaagact atccagtaga aggtatcagt
     8221 gaaaaatatc gcggacgtgt tttgtctttc ttattgatgc atgtagtgac acaacgcatc
     8281 gatgctcatg tggagcagtt gaactgccat ccattagaga tacgccgcta ctatcgtcag
     8341 tttgattatt aggatagact ggataaaaag aatataaaaa ataaagagag cagcgataac
     8401 tgctcccttt atttttttaa aatattggca tcaattacgt tataaaacac attttttgca
     8461 ttatctacag ttcaacttat aatctgttta atgggacatg tgggtgccaa ccattttttg
     8521 ttatgcaata gtactattta gttgttgcat ggatccgtag taaaagtcca agtacaacga
     8581 gttgcgccag tttacatagc gttttgtatc cagaatgttg actaaaacaa aagttcctat
     8641 gtcaaccgtc aacagagaca atctgtccgt tgcaggacct gacagattta gaaatgttca
     8701 atctgtctct tatgtatttt aaatcaatac tcactaggag aagtttccta acctcctaat
     8761 agtaatgctg gtagtaacat tgtcaaaact gcttcctctt aggaacaagc aaagcataac
     8821 gagtgtaaaa ataaaatgta gactattgta acatatcatt gattatttca agtaaaaacg
     8881 ataaaagaag taaacaaaca cttaaaaaca atggaaatta gtcccaactt taagaaatgt
     8941 atactataat ggaaattatc aattatttat ttggaggaat atccatgaaa caaaaaatcg
     9001 cagttcttgg tcctggatct tggggcactg ctttagcaca aacattggca gaaaacgggc
     9061 atgatgtccg tatttgggga aatgtacctg aacagattga tgaaatcaat aagtatcata
     9121 caaatcagca ttttttacct gatttgacga tcccagaatc gattatcgga tataaagaat
     9181 tggcagaagc agtggataac gcagatgcta ttttatttgt tgttccaaca aaagcgattc
     9241 gttcagtcgc acaggaactt attggtaaaa tgaatactaa gccaatcatt atacatgcaa
     9301 gtaaaggact agaacaagat acgcataaac gaatctcaga agttttggct gaagaaattc
     9361 cagaagaaaa aagacaggca attgttgtat tatctggtcc tagccatgct gaggaagtgg
     9421 cagtacatga tatcacaaca attacagctg caagtattga tcaaaaagca gcagcctatg
     9481 tacaaaaact gttcatgaat gattatttca ggatttatac aaataatgat gtgattggcg
     9541 tagaaactgg tgcagcactg aaaaatatca ttgcaattgg tgccggcgct atccatggat
     9601 taggattcgg agatgatgca aaagcagcaa tcatgactcg aggattggca gaaatcagcc
     9661 gtcttggtgt cgcgatgggt gccaatcctc tgacatttat cggtttgagt ggtgtggggg
     9721 acttgatcgt tacttgtaca agtgttcatt ctcgcaattg gcgtgccggt aacttgttag
     9781 gacagggaca taagctggaa gaagttctag aaaacatggg aatggtcgtt gagggtgtgg
     9841 caacgacaaa agcagcagta gagctagctc aacagctagg tgttgagatg ccaattactc
     9901 aaacaatcta taatgttttg tataatggag aagatatcaa aaaagcagca aaagaaatca
     9961 tgttacgtga cggaaaaatg gagaatgaat tttctcttcg ttaaatagat gatcaatagt
    10021 taatcgt
//
LOCUS       DAZUKZ010000095         9968 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000095 DAZUKZ010000000
VERSION     DAZUKZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.097"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(23..1534)
                     /locus_tag="ACG8EF_002199"
     CDS             complement(23..1534)
                     /locus_tag="ACG8EF_002199"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4947488.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(1856..3208)
                     /gene="murF"
                     /locus_tag="ACG8EF_002200"
     CDS             complement(1856..3208)
                     /gene="murF"
                     /locus_tag="ACG8EF_002200"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFS4947489.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(3361..4437)
                     /locus_tag="ACG8EF_002201"
     CDS             complement(3361..4437)
                     /locus_tag="ACG8EF_002201"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS4947490.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            4633..6378
                     /locus_tag="ACG8EF_002202"
     CDS             4633..6378
                     /locus_tag="ACG8EF_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFS4947491.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(6658..7095)
                     /locus_tag="ACG8EF_002203"
     CDS             complement(6658..7095)
                     /locus_tag="ACG8EF_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFS4947492.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(7079..8452)
                     /locus_tag="ACG8EF_002204"
     CDS             complement(7079..8452)
                     /locus_tag="ACG8EF_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947493.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(8774..9262)
                     /locus_tag="ACG8EF_002205"
     CDS             complement(8774..9262)
                     /locus_tag="ACG8EF_002205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4947494.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(9284..9886)
                     /locus_tag="ACG8EF_002206"
     CDS             complement(9284..9886)
                     /locus_tag="ACG8EF_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4947495.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         2916 a         2125 c         1802 g         3125 t
ORIGIN      
        1 aagagaaact ttcattttat gtttaatcgt tatttgaacg gattacaaat ccgcgttttt
       61 tatcattgtg acgtttaccg ccatcttttt ggctgttatt atttttgcta taaccgcctt
      121 ttgatttgtt tccacgataa ttaccgccgt cacgacgatt acggttgttt ccgccaccac
      181 gtttgccatt tttattgtat ccttttttct gcattggcaa tgggcgttct ggtgtgatct
      241 tcactggcac tgcatcagct ggatcttttg ctgtcgtttt tagcaaaagt gcaactaaat
      301 cttgtgcaga atattcttct aataatttat ctgctgtttg caggtattta tctagaccat
      361 tttctgcaag ttttgtttcg atttgttcaa ctgcagcacc taattgacct ttgaatgctt
      421 ctttttcggt tggtggacgt aaagtcgtca tccgtttttt cgtcaagttt tcaatcacat
      481 gcaagtagtc catttcattt ggtgtaacga aagtcacaga cataccgcct tttccggcac
      541 ggcctgtacg tccgatacgg tgaacgtagc tttctggatc ttgagggata tcatagttgt
      601 acacatgagt gacacctgaa atatctaatc cacgtgcagc aacgtctgtc gctacaagga
      661 tatctaaatg accgctctta aatgaacgca atacgctcat tcgtttttgt tgagacaaat
      721 cgccatggat tccttctgca cggtaaccgc gtgcttctaa tccgcgtgcc aattcgtcta
      781 cacggcgttt tgtacgtccg aagacgattg ttaattcagg tgtttgaaca tcaaacaagc
      841 gagtcatgat atcgaatttt tcgtattctt ttgcgcggac atagtattga tcgatcaagt
      901 cagctgtcat ttctttcgct ttgatcttca catgatgtgg atttttcatg aatttaacgc
      961 caatattctt gatagctggc ggcatcgttg ctgagaaaag taatgtttgt ctttgatcag
     1021 gaacttgtga aatgattttt tcgatatctt ccaagaagcc catattcaac atttcatctg
     1081 cttcatccaa aactaatgtt tgaacggttc caagtttcaa tgtgtggcgg ttaatgtggt
     1141 caagcatacg tcctggtgta cccacaacga tatgtggacg atctttcaag ccacggatct
     1201 gacggccaat atctgctccg ccgtaaacgg cttgtacacg tacttttttg tcgcgcccta
     1261 aacgatataa ttcttcttgt gtttggatag ctaattcacg tgttggtgcg ataactaaac
     1321 cttgtaattc gtgacgatct ggatcgattt tttctaacat tggtaatcca aatgcagctg
     1381 ttttccctgt acctgtctgt gcttgtccaa taacgtcttt tccagctaat gctagtggaa
     1441 tcgtttctgc ttggataggc gttgcttctt caaaaccagc gcgttcgact gatttcaaca
     1501 attctggtga taattctagt tctttaaatt tcaaatgaat cctcctatat ggttgttaac
     1561 tgaggatgta gcttttttgc taccccgtcg atttgttcgg tcaaagtgta ttgtttcttt
     1621 ttgacagtgg ccacgcacgt ttttatccat tcttagcccc tgtggcacca ccgaaaaatc
     1681 actttgcgaa gtttaggcat tagaaaagcc aaacgggtcg cgttcagctt tatcattatt
     1741 atgacatctg gcttcaacaa gttgtttcta tggcaaaacc agtccaattt ctcttcatct
     1801 ttcggcaata ggttattcta acatagtttt tcgttttctg caagtttaca gatttttatt
     1861 tcatttgctg aagttgttct accacttcaa ataagcccat gctgttgctg ccttttagca
     1921 taatactatc tgttgcttca agattttgtt tgatagcatc aatcaattta ttcttttctt
     1981 tttcaaaata gaacacaggt agttgggggt atttttggtt caattcttct tttagtgcat
     2041 gcatttgttc cccataaagg aaaatcatgg cgaatttctc aggatcgata tgttcactca
     2101 tttgcgcatg catatttaaa gactctttcc ctaattccag catatctgct aacacagcga
     2161 ttctgcgtcc ttttgttggc agatttttaa aagtatctaa taccagtccc atggctgttg
     2221 gatttgcatt gtagacatcg ctcaaaatag ctgcaccatt tcctgccgtt agccattcgg
     2281 ttctgttttg agtgatagaa acatgggcga gcccttggcg tatctcaggt atggacagtc
     2341 caaagaagcg cccgatcgta tacgcaatca aagcattcgt tacattataa cttccaggca
     2401 gcgggatcgt aaatgtttct ccttctacta caaaatcggt ttgtgctttt ccttttgctg
     2461 tcacttccgc atgaacatcg ccatttccaa taccgaatgt tgtgactgtt tgtactaatg
     2521 ttgccgttaa aggtgtcagc aacggttcat ctgcgggcac aacgagtaat ccgtctgata
     2581 caagtccgtc agtgatttcc attttggctt tagcgattcc ttctcgcgac ccaagatttt
     2641 ctatgtgtgc ttcaccgatc attgtgattg ctgcagcttt tggttgagcc ataagtgaaa
     2701 gttccgtgat ttcattggcg tgatccatgc ccatttccaa aacgatcttt tctgtatctt
     2761 ggggcatgtg caaaatcgtg taaggaagac caatattatt gttgtaattt ccttgcgtct
     2821 tatatgtttt gtatttttca gacaagacag acgcggttag atctttcgtg gtggttttac
     2881 cattgcttcc tgtaattgca acgacttctg cgcctacttt ttccaagtaa taggttgcca
     2941 attgctgcat ggctttcaaa ggatctgcta caggaataac tggaaaattt tttggagctt
     3001 gttcaacagg caagctccaa aatgaagcag atgctccgtt tgtgatcgct tgctggataa
     3061 attcatgtcc atctcgcgta ccagcaagag gaacgaataa acttccttcg gttatcttcc
     3121 ggctgtcaaa ttcgacattg gtaatctttg tcagtgcaga gcattcgctg cccacggctt
     3181 gtgcaatttc ttgtattgtt agattcattg agatctctcc taaaaaaggt tgtgaaaaaa
     3241 gcgtttggcc taaacggcgg gttcgcatga ctctcacttc caagtaaatt tctctattcg
     3301 tatttagaag ctagaatgaa cgcggttacg caatcactcc agcctcttct ttatttctct
     3361 ttattcattt ttttcaaaaa aagattgacg ctgatggtat cgattcattc ctaactggat
     3421 cagttcttca atcaaatcac cgtatttcaa gcccatattt tcccataaga gtgggtacat
     3481 actgaactcc gtaaatcctg gcatagagtt taattcattc aggaataatt catttttatt
     3541 tgtcaaaaag aaatcgcacc ggctcaatcc gcttccacct aacatcgtgt aagctaactt
     3601 cgcgtactct tgcgcttttt gataaacttc ttctggcact tcggctggaa tctgcatttc
     3661 gattttatta ttgatatatt ttgcttcata atcatagaat gctacgtctt ttacgacttc
     3721 accaggcaaa gtcgtccgaa cgtcttcatt tcctaataca gcaacttcga tttcgcgcgc
     3781 ttcaattcct tgttcaacga ttgctcgaga atcatactga taggctgttg ctaaagcatt
     3841 ttgcagctct tctcggtttt ctgcttttgt aatgccgaca ctagaaccca tattcgccgg
     3901 tttgacaaac atcggataaa gcaaagaacc ttcacattga tcaaatactt ttttaggatt
     3961 ttctttccat tgattcttaa gtactggtac ataaggaact tgcggcacac cagcagcttg
     4021 taaaatatac ttggtcatga ttttatccat ggcacatgca ctggtcaata cgcctgcgcc
     4081 gacataaggc atattcaatg tctctaagaa gccttggatc gttccatctt ccccgtttgg
     4141 cccatgtaaa actggaaaaa cgatggctcc ttcttctttg atttcgcccg gattgatcac
     4201 ttttcctgta aagccttcct ctgtctgtcc acttgggtcc catgaaagat gcaagacatc
     4261 tttgctggca ggtttttctg ttagtaatgg acctttgacc cattgtcctt cttttgtaat
     4321 aaatacgagt tgaacttggt aataattata ataaatggca tttaaaactg aaaatgcgga
     4381 aagaatggac acttcatgct ctgcgctgcg tccgccatat agtaaagtaa tcttcaaaag
     4441 attgtcctcc tgtattctaa atcgttcagt gatttactct catattttag cacaaaacaa
     4501 aaataaacga ataacttatt tataaaaaaa cgagccagct tctaaaagaa cgagcgtcat
     4561 ttattttttg tcagctagat tatttgtccc gttttttata aaaagttatg ataaagaaaa
     4621 ggagggatga tgatggcgca aacaaatcca cgcagccgct tggcagaaat cattcgagtg
     4681 ttcatcaatt acaatgtagt gccaaacttt gtacagcaaa aaaatcccga acaggtaaaa
     4741 aaagcctttg aagaactagg ccccaccttc atcaagatag gacagatgct ttctgttcga
     4801 gaagatttgc tttcttctgc cttcactcaa acattcaaaa cattacaaga tagtgtacct
     4861 agcgacacat tttcaacggt caaaaagacg atcgaaacag aactttctct ttcattatca
     4921 gatatattcg atgatttttc aaaatcgcct tttgcctctg cttctatggg gcaggctcat
     4981 cgtgccaagc tgaagaacgg tgacgcggta gtcgttaaga tccaacaccc taacatcgcc
     5041 gaagagatcc gacttgatct tcaactattt gaacgagcga tccctttgat caaatacatt
     5101 cctgaaacta gcgtagttga tctaaaaggg gtactccaag aagtaaaacg ttccttgatc
     5161 aatgaaatgg acttcttaaa agaaagtcaa aatggcgaac aattctatca aaagaacaat
     5221 ggctggaaag agatccgcag ccccaagatt tatgacgctt tttgttcgaa aaaagtgatc
     5281 gtgatggaag aaatgtctgg taaaaacttg aatcatttaa tgaacgccga aaacaaaact
     5341 gaaactttta tcactggaat tcaaaacaag cagctgaaac aagaagttgc aaagctttta
     5401 gttgagaact ttatgaaaca ggtgtttgat gatggttttt tccatgctga tcctcatcca
     5461 gggaatctgc tattccatgt actaacaaaa gaagaacaga cacaagctag ccgaaaaact
     5521 gaaacagtcc atgaaaaaga gttcggttcc tttgcttttc gagcctccac ttctgcagaa
     5581 gaccctgttg cgccatatac catcaactac atcgattttg gtatgatggg gcatctttct
     5641 gcaggactta ggcaaaaact cacacaagcc gttttagcgc tgtatacgaa agatgcttat
     5701 cgaatcgaaa aagcggtcct tcgactttgt cagcaagaag gctcgtttga tgaaagtcgg
     5761 ttccaccaag aactgacaag ttttcttgaa cagtattatg attctcctat tgatgaaatc
     5821 aacttgcaag aagtattcac acatgtcgtg acgatttgtc atcaaaataa tctgcagttc
     5881 gatcgtgata tcacgctttt gctgaaagca tttgggacat tagaaggggt gatccgcgtc
     5941 ctcgatcctg aagtttccct catggaagtc gcttcgcctt ttgctcaaca ttattttctc
     6001 actcatctgg atgtagaaga tacattgaaa caaagcggat tagacttatt ggaagggatg
     6061 aaggctgcgc ctaagatccc tcaacagctc caccatctat tagaaatgtg gacttcggga
     6121 caaggaaaag tcaatttgga attgaaaaag caagacaaat tattgagcag gatcgaaagt
     6181 atgatcaatc ggcttgtttt cggtatgatt ttagctgcat tgatcgtagg gtcctccctg
     6241 cttgtccaag cagcacctgt agaaaacgca gaggtcgttt ccttattagg gatatttacg
     6301 tatgctattg ccgcattcgt cattattttt ttggcaatcg atgcattgat ccaaatgtat
     6361 aagaagagga aaaagtagaa aaaggttgtg acagaaagaa tcagcttcaa gaaataagaa
     6421 ggggttttac ataaattgtt tctcaaattt ttgtgaaaat tcggcttatt tttcgaagag
     6481 gctacttctg aaacaccgag aataaaaaat gtgaaaaagc tgttctgcac ccagtaataa
     6541 gaacaatcaa aaagggaaca tggaacacat ttatcctgtt tccctgttcc ctttttgatg
     6601 tattcgagca taaataaaat accttcgtta cctactatct tctgtacttg gtaaacctta
     6661 gtcaattgcc ttttttaacg cttgacgaaa agacatatga tctttctccg taaagggttt
     6721 tggacccttt gtccttatgc cagcttttct taactttcca ctatgataac gttgttctag
     6781 aagcgtgccg atattcgctt cgtcaaattc ttttccagag tgatggaaca ctcgcacacc
     6841 cttcggcaat acaagtgaag ctaaaccata atcttgcgtg atcaaaaagt cattcttctt
     6901 aaccataccg acgattttat aatccgcact atcagctccg cggtctacgt agacgatttg
     6961 gacatgatta gcgtattctt tggtcgtata atggtccaca ctggtcacga taaccacgtc
     7021 aagaccatag gattctccta attttatgac atcttctttt actggagaac catctccatc
     7081 aatgaatagc ctcattcttt ttcgccttct tccataaatg ataaacagtt actaatccgc
     7141 tcagtaaaag accaacattg atccattttg ccaagataag cagttgtaca ctttctatcg
     7201 gcactgattc atggttcagc gtgatcaata atagatggat tggctctccc atttcttttg
     7261 ctaaaacaaa caaaagcgtc atcaataaaa actggtggat cacactccct ttattttgag
     7321 tagtatactt gacaacgatc cggcgatcca ttggaatagc tgtatcagtt tgataatatt
     7381 ccttatttag ccagctgcct gttactacgt gccagaaact aagcagaaag acacctatcg
     7441 caaaaccatt tggaatcaag aggaccaata aagaagaact cagtccaagc aattgcatag
     7501 ccaatggaga ccatgttaaa cgtttcaaaa tccatggtcc gactattttt gctgcaatca
     7561 agcctgctgc ataaggcaaa tacataaaaa tcgaaagttt ttctaatccg tacagtatgc
     7621 caacataaaa actgctaaat aaaaaaagga aattccctag cattccattt agaaaagtaa
     7681 acagatttag ccagcttggc aacttccatt ccttcttctg gtgtcctaaa acaaacacga
     7741 acaagatgaa cagcagcaag aaaccataaa tagcaagatc aaaaagcctt tcatcgaata
     7801 acaatcgggc atttctcaaa agaaacaata agccaaagaa tatcaaaaat aaaacaagct
     7861 ctcgcgtagc taccagattt cttttgatgt ctttgaaatc cagttcgtaa cgcggataat
     7921 gactaaccgt atggtaggcc atgacgtaaa aaagtgtata aatagtaaag cttgctgcaa
     7981 tctgagtcgg tgcaggcaac tcaatcgctt gatacaggag aaatagccag atagctgcaa
     8041 ataagtactt tttcccatcc atattgataa aaccttgcgt cttttcgtga aaattgaccg
     8101 tggtattagc aggtacaagc catgctgcac tcagccctag caaaacactt gaaaaataaa
     8161 ataaaggaaa ataaaatacg cctaatattc caagcaatga accagcacca ccaatcagca
     8221 gagcgatcat cagcaacgtg taactatcta gaccaaatcg tagactgttg actaaaaata
     8281 gtccagtcat ccgaaaggca taaaacagcg taaatggcaa aatcgtcagc caattatgcg
     8341 agtctgttag tcccaaaaat aaaagataag gaataaaaat aaccgtgtta cttaagataa
     8401 agggcgttgt ttgtgtaaaa ttacgcccaa atcgtttgag tttttcgaac atgacaatgg
     8461 ctccttaaac agtcttataa ctaatctgct cttcgatttt agcattaaag gggaaataaa
     8521 ccaattttac agaatatatt cttgtaaaaa aaaatacaga aagaagcata agcttctttc
     8581 tgcattttta cggtacttga ttactcaagc agacaagccc ttctccacaa aagtcttgcc
     8641 cgacacccct gcatccggca ggtgataaag atagaaatca attatcctgt gcctccaaaa
     8701 tcaattagga gctctgtaaa ccagagtcct tctcgacccg tcgcataaat ttactttaac
     8761 acagttccta tgtttatgcg agagcttttt tcattttata atcgaccatt tgaaatcctt
     8821 gaccgatatc tgaaataagt tcttctgtaa tctcaaatcc ttcatgctta taaacctcga
     8881 ttgcttgatg attcccctga ttcactcgca aaaagatctc tctttccttt gtttgcgcag
     8941 caagttcttc ataccagcga aaaactgaag aagtcaatcc ttgaccacgt actttattta
     9001 gcaaatataa tttacttaaa taaatttttc catttatctc ttcataagca gtgtaaccga
     9061 cagtcttttc ttcgcttttt aataaaaagt aggacacacc ttctgtcagt tctttttgaa
     9121 tctgtttttt tgactgataa gttttcagca tgtaatccac ttgtttttga ccaatgatcg
     9181 gcacaaaaac ttccggccat acttcttgta tgactgccat cagctcatcc agttcatttt
     9241 ctgttacttt tattttttcc atacacccac gtcctttttt acattataaa tgaccttgcg
     9301 cttgatggaa taaaaagagc atgatcatgc tatacacacc caccgaccac ggtttcaaga
     9361 aaatcacaat ctgcataaat tccctaaaag ataatttcgt caaaccagct actagacaga
     9421 tcaagtcgtc tggcgcaaag ggaagcagca aggtaacaac tagtactttt ttaatatctt
     9481 tcgtttttct tgtcagcaat tcatcaaatt tctgatactt ttttggcgat aaaataagct
     9541 ggacaaaggc tacgccatag taacggacaa gcaaaaatcc gattgcttcc ccaatcacta
     9601 ggccaataca actatataaa agcccaaatc cattgccaaa catcagcatg cccgctactg
     9661 aggagatccc tcccggcaaa ataggaatga tcacttggat cacttgcaac agaacaaata
     9721 tcatcactgc atacttgcca aattgctgga taaacgcttg caaagacgtg ctcgatcgaa
     9781 aaatcccatg atgataagca taaagaatca acacaaagaa aaaaaacaga ccaataatgg
     9841 gcatgatctg aagacatttt tgtaaaactg atcctttttt agacatctat attccccaca
     9901 tcttttttca tttattaaaa ctgcctcctt atgaactgaa ccccaaaagt tgaactattt
     9961 aatggact
//
LOCUS       DAZUKZ010000096         9950 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000096 DAZUKZ010000000
VERSION     DAZUKZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.098"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..251
                     /locus_tag="ACG8EF_002207"
     CDS             21..251
                     /locus_tag="ACG8EF_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFS4947496.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            348..1649
                     /gene="murA"
                     /locus_tag="ACG8EF_002208"
     CDS             348..1649
                     /gene="murA"
                     /locus_tag="ACG8EF_002208"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS4947497.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1662..1838
                     /locus_tag="ACG8EF_002209"
     CDS             1662..1838
                     /locus_tag="ACG8EF_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS4947498.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            1973..2269
                     /gene="yidD"
                     /locus_tag="ACG8EF_002210"
     CDS             1973..2269
                     /gene="yidD"
                     /locus_tag="ACG8EF_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFS4947499.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2333..2800
                     /locus_tag="ACG8EF_002211"
     CDS             2333..2800
                     /locus_tag="ACG8EF_002211"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4947500.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            2885..3769
                     /locus_tag="ACG8EF_002212"
     CDS             2885..3769
                     /locus_tag="ACG8EF_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4947501.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            3883..4119
                     /locus_tag="ACG8EF_002213"
     CDS             3883..4119
                     /locus_tag="ACG8EF_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFS4947502.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4207..4287
                     /locus_tag="ACG8EF_002214"
     tRNA            4207..4287
                     /locus_tag="ACG8EF_002214"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4241..4243,aa:Tyr,seq:gta)
     gene            4294..4365
                     /locus_tag="ACG8EF_002215"
     tRNA            4294..4365
                     /locus_tag="ACG8EF_002215"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4326..4328,aa:Gln,seq:ttg)
     gene            4490..4660
                     /locus_tag="ACG8EF_002216"
     CDS             4490..4660
                     /locus_tag="ACG8EF_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947503.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            4880..5587
                     /locus_tag="ACG8EF_002217"
     CDS             4880..5587
                     /locus_tag="ACG8EF_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4947504.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5647..6291)
                     /locus_tag="ACG8EF_002218"
     CDS             complement(5647..6291)
                     /locus_tag="ACG8EF_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFS4947505.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6418..6705
                     /locus_tag="ACG8EF_002219"
     CDS             6418..6705
                     /locus_tag="ACG8EF_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947506.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6813..7133
                     /locus_tag="ACG8EF_002220"
     CDS             6813..7133
                     /locus_tag="ACG8EF_002220"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4947507.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7214..8527
                     /locus_tag="ACG8EF_002221"
     CDS             7214..8527
                     /locus_tag="ACG8EF_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS4947508.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8524..9243
                     /locus_tag="ACG8EF_002222"
     CDS             8524..9243
                     /locus_tag="ACG8EF_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFS4947509.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9371..9922
                     /gene="raiA"
                     /locus_tag="ACG8EF_002223"
     CDS             9371..9922
                     /gene="raiA"
                     /locus_tag="ACG8EF_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFS4947510.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3325 a         1653 c         2138 g         2834 t
ORIGIN      
        1 tcgttagaaa ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga
       61 gtcatttggc gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt
      121 ttttcagacc aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt
      181 tagggtacac tgcaagtaat tttttcttag aagttatcgc tttatgccga aatcttttcg
      241 ttagcctgta aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa
      301 gattttgagt aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca
      361 tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg
      421 ttttgcctat tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg
      481 ttccgattct ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg
      541 tagattttga tgaacaaaag aatcaagtaa caatcgatgc ttcaagacaa ttagaaatcg
      601 aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac
      661 ttgccagaaa tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac
      721 caatcgattt gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg
      781 gctatatcga agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta
      841 gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca
      901 ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg
      961 gcgctcaagt ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac
     1021 atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg
     1081 ctgcaatgac acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat
     1141 tgatttctaa attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg
     1201 tcatcggacc aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc
     1261 ctacagatat gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtag
     1321 tgactgaaac agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg
     1381 ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg
     1441 cagaagtata tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag
     1501 caaatggaat cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc
     1561 atatcaaact acagcaatta ggtgcagacg ttgaacgtgt agacatggat caaacttctg
     1621 ctgaaaaaac agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata
     1681 tcttattaac tttgataaag attttagtgg tgatcttatt gcttatcctt ctttttgtgg
     1741 ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtattccagc
     1801 caagtttgtg gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc
     1861 tagtcgatat gaagtgagcc atgaagcaga acatgacaaa agaaaacaga agggaacgtt
     1921 gacgttagtc gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa
     1981 gatattgatt tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag
     2041 ctgccgttat tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggtgc
     2101 actaaaaggg acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg
     2161 cggtatcgat tatgtaccat tgacattcca attaacgcga aataagaaag ataaaaaaga
     2221 accagattat aaaaaggttg tgaaaaatac gagtagcact gagcgataaa gaaaaagagg
     2281 aaaattattg tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga
     2341 agaactaaac aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga
     2401 aaaagttcaa gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac
     2461 aataggtgta ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt
     2521 agccccaacg tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa
     2581 gctttcacaa ctcgcttcca gtcaaactca gatcatcata cgaaccggca gtactagcag
     2641 tgcggcgtta catctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta
     2701 ttttatccat cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt
     2761 aattttagca attcacttac ctaaaaatag ctttaactaa taaactttca attcgagaaa
     2821 atgctttcaa atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat
     2881 ttttatggga atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga
     2941 tggcaaagag attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa
     3001 agcgcaatta tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg
     3061 aatcagctct cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat
     3121 tccttatatt catggattta atattttccg agatttggaa actctgtttc aactcccagt
     3181 aacgattgaa aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg
     3241 tgattatcaa gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac
     3301 gaaaggacaa atcaataaag gggcacatct ttatggtgga gaatttgggt tgatgttctt
     3361 aaatggcgat aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg
     3421 tgaacgcaaa ggtctcgata aaaattctta taccggaaaa gatgtctttg aattagcaga
     3481 aactggagat gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg
     3541 attattcagt attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggtgtttc
     3601 tgcaaaagaa gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt
     3661 cgatctgcat gatttcgaac cgcagatttt attatgtgaa tacagaaacg atgctaatct
     3721 tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg gagaattaag agctttagag
     3781 aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag gagattttta cctaaggaat
     3841 gttttatttg ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat
     3901 aaagatgttg aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa
     3961 gtcactcagt tatttatagg gaaaaaaatg attggtgaga tcttagccta tgggccaaaa
     4021 gaatatgaaa ttttctttgg tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg
     4081 atagaaacag tgattcgaca ctggaatctg cacgaataaa aaaatatcaa aaaaactttc
     4141 aataaagggt tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg ttgatacaaa
     4201 cctttcggag gagtagcgaa gtggctaaac gcgacggact gtaaatccgt tccttagggt
     4261 tcagtggttc gaatccactc tcctccattt cattggggta tagccaagcg gtaaggcaag
     4321 ggactttgac tccctcatgc gttggttcga atccagctac cccagtatac cacaatcgta
     4381 agctctcgtt agttcgagag ctcttttttt tgctttgcta ccttgttcct tgaagattct
     4441 gctgatttaa gatagagtta agttaactac aagaattgga gggatccaca tgaaatggaa
     4501 agaaaaagta gacgaagcag ctgaaaagtt atatgatttg gtcaaatcag aaaaatacaa
     4561 tattgaagtg aatattccga aaaaaggcgg aaaagcggtc caggtaaaat caaaacgccc
     4621 aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa taataacaaa agatccataa
     4681 gtcggcattt tcatcgattt tagggtcttt tgttattttc taggttgaac atatctaaaa
     4741 aattgtttct gacaatagga gtaggaagaa cgatgtgtgt ctaaatcctc tgttgatgga
     4801 tagaaacctt tatctttttc agttgtgaat caggtatata ccagttataa tagaagaaac
     4861 gaaaggacgg gtgtacgtta tgtcgaatca attacctgta tatatacaga ttcatgacca
     4921 attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga gaccgtcttc cttcagaaag
     4981 agaattagca gtgaaattca atgtcagccg tatgacgttg agacaagcaa ttcaaaactt
     5041 ggcagatgaa ggaatcttag aaagaaagat cggttcgggt acatatgttg cgcgagaaaa
     5101 agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa attatggaat cgcaaaatcg
     5161 agtaccgtct agtcggacaa tctcttattt cttgacttcg ccaagctcaa gtgaaatgga
     5221 aaaactaaac ttgagtaaag atgacacgat cttgcgaatg gaacgtattc gtttcgcaga
     5281 tgatgtccct atttgttttg aagttgccag tgtgccacaa aagctgatcg atggctacag
     5341 caaagcagag attactgaat cgttgtatcg cacgctagaa gaaaaaggcg ggaaaaagat
     5401 cggtccagcg aatcaaacga ttactgctgt gttggcttct gagaaaattg ctgactattt
     5461 agaggtaaaa cgaggagatg cagttcttcg tttgcgccag atttcttatt ttgaagacgg
     5521 tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat cgttttgagt tttatttgga
     5581 aaaataaaaa acaggtgatc cctgattcct tacttagaaa taaaagagaa tcagaatcac
     5641 ctgtttttat atatgatcag ttaccagttg ctcgtgataa gaaacaggac cctcttcaaa
     5701 ttggacttgt ccgttcagca gctcaatgat ggattcttta aagtggtcag cacttttttc
     5761 atctacagca cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg
     5821 ttggagttca agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat
     5881 ctcctgttgc aaggtacctt ctacgattcc aatgtcagac aatgcttgag agacagcatg
     5941 agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac
     6001 tactgcagct acattgatca gttgattttt tttgaggact tccaacatcg gtacgccagc
     6061 cgttccactt ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat
     6121 gaaggcgctg cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaact
     6181 ttttgcttgg tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat
     6241 ctcaatctca ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc
     6301 tctttttttc attttcttta ttcagtataa ataaagttct aggttgtttc aacactgtaa
     6361 cggttgcgca atcgctgtag tatgcgataa taaagacaaa ggtaggtgga tctggggatg
     6421 caggaacgaa tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa tggcggctct
     6481 caagtaacgt tgacctttac ccaatttgac gaactgctgt ttcccagtaa cggacttccc
     6541 aaaacagcaa gggaaagtac agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt
     6601 gcctatggct ggataaatgc aggctatgaa gtagttgtca tcaatttgga caaagaatat
     6661 gtcgtgttca ataaattagt gaaatctagt tggctatttg attaaataaa ccgcgtttag
     6721 aaaacgttga caaatttctt tctttttata taatagagta acagacgcga agaaaacgtt
     6781 tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa aaaaacaatc atgcttgtat
     6841 gttcggctgg tatgagcaca agtttactag taacaaaaat gcaaaaagct gctgaagcaa
     6901 aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa
     6961 ataaagatgt agatgtactg ttattaggac ctcaagttcg ctttatgaaa gctgattttg
     7021 aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat caatatggct gattatggta
     7081 tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct cataggtgaa taaatagaac
     7141 agtataacta tcgaacaagc gatccaaaaa atagatcgct tgttttttcg tatatttttt
     7201 tagggcgaat aatatgaatc atttttgggg tagaaggctt ctgacgagag atattaaagc
     7261 aatagactgt gaggaggtaa atttaccaaa ttatgagaag atcgcagcag tggaacgttt
     7321 gggcaactgg attctttgtc atcgctgtgg aatgaggacg tccgtcattg atggacagct
     7381 tgcagattac ggttattttt gtacacattg tctatattta gggcgatgcg acactcaaca
     7441 agatttatat ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct ttttttggac
     7501 aggacagctt actgaaaaac aaacggaaat cgcaaagaga atactttgcc attcagaaaa
     7561 aagacatcat cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat
     7621 tttagtggaa actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt
     7681 ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct
     7741 ccatgggaat acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca
     7801 gctataccgt ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc
     7861 ttataacggg gatgcaggat tagctcatgc tgtgcaaaca gcgattaaac cagacggacg
     7921 attcatctac ttaagtgcca cacctgataa aaagttacta aaggaaatca agcagacgtt
     7981 tgacatccac aaacttgctt tgcgctttca tcggcgttta cttcctgaac ccgtcttggt
     8041 tttcatgaat aattggcgac aaaaatgcaa gaacaagaag aagataaaat ctttggtgcg
     8101 gatcattcat caattaatta aagaaaacca tatcctttta ttttgtccaa gtatcacgat
     8161 gatgcatcgt ttgaaagcca cacttcaaga ggctcttccg agatatgaga tcacagatgt
     8221 ttcttcaaga gacaatcagc gggcggaaaa agtccagaaa atgcgagaag caaaatatga
     8281 catactgtta accacgatga tccttgaacg tggagtcaca tttgaaagaa tatccgttat
     8341 cgttttagga gcggatcatc cggtttttac taaatcttct ttggtacaaa tcgcaggaag
     8401 agccgatcgt aaagggcctt atacgaacag tcaagtatac tttttctatg aagaaaaaac
     8461 tgcagctatt cgaaaagctt gtcaggaaat caaagaaatg aatgaagaag ggaagaagat
     8521 gctgtgaggt gcagttgctg cagtaaagat atcgtccaaa atttaacatt cagcgagatt
     8581 attggtgttt cgaaatacag attacctcag acttgttcag tttgtcgcgc acaatttcat
     8641 ttattggaaa caagaggctg ttctggctgt caaaaaccaa tccatttcaa tctagagagt
     8701 acgaaaaaga aagtaaagtc tattcccgtt tactgcagtg actgtcaaga atggaagaag
     8761 aaatatccga cctatgattt tcagcaccgt gccttttttg tgtacgatga aaatatgaag
     8821 gcctggctca atcaatacaa gtttatggga gatatccgac tagcaggcac ctttgcatat
     8881 aaatggacgc agataaaaaa agaatacccg acatacatct attgtccaat acctttatct
     8941 gaaaaaaggc gaacagaaag aggattcaat caagtaagcg agatgttgaa agcagcaaga
     9001 attccttttc agatgctact caaaagagag aagcatctgc tggctcaagc aaaaaacacg
     9061 cgaaaggaaa gattggagat gcctcaacct tttgaacttg cagtcacctt ttctaagata
     9121 caaaataaaa aaatattgat tattgatgat gtctatacaa caggacagac tcttttccac
     9181 gcagcagatt gtttgcaggt cgcacagccg aaaatcatcc gaaccatttc tttagcaaga
     9241 tgaattagat gtggaaattt gatagcgatt taatttgcaa ataacctaga gttcgatata
     9301 atagaattaa gaagagggaa agagtggtcc ttctccttct tgattgtggt agacgaaagg
     9361 ggtaattttt atgtttagat ataatgtacg cggcgaaaat atcgaagtaa ctgaagcgat
     9421 tcgcgactac gttgagaaaa aagtaggcaa actagaacgc tatttcagtg acgcacccga
     9481 tgcaacggcg catgtgaact taaaagttta tacagaaaaa acagcaaaag tggaagtaac
     9541 gatcccatta ccttatcttg tattacgtgc cgaagaaact tctccagatt tatacgcaag
     9601 tatcgatttg gttgttgaca aattggaacg ccaaatccgc aaattcaaaa caaaaattaa
     9661 tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct actgctttat tcaatgaaga
     9721 ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt acgaaacgcc tttcattgaa
     9781 accaatggat agtgaagaag cagtcttaca aatgaacatg ctgggacata acttctttat
     9841 ttttgaagat gcagaaacaa atggtacaag tatcgtttac cgccgtaaag atggaaaata
     9901 cggactgatt gaaacagact gatcagcctt acttaaataa caacgaaaac
//
LOCUS       DAZUKZ010000097         9870 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000097 DAZUKZ010000000
VERSION     DAZUKZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.099"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            205..684
                     /locus_tag="ACG8EF_002224"
     CDS             205..684
                     /locus_tag="ACG8EF_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFS4947511.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            717..2945
                     /locus_tag="ACG8EF_002225"
     CDS             717..2945
                     /locus_tag="ACG8EF_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4947512.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3074..4717
                     /locus_tag="ACG8EF_002226"
     CDS             3074..4717
                     /locus_tag="ACG8EF_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS4947513.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4790..5335)
                     /locus_tag="ACG8EF_002227"
     CDS             complement(4790..5335)
                     /locus_tag="ACG8EF_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFS4947514.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5358..5843)
                     /locus_tag="ACG8EF_002228"
     CDS             complement(5358..5843)
                     /locus_tag="ACG8EF_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4947515.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5833..6645)
                     /locus_tag="ACG8EF_002229"
     CDS             complement(5833..6645)
                     /locus_tag="ACG8EF_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFS4947516.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6784..8316)
                     /locus_tag="ACG8EF_002230"
     CDS             complement(6784..8316)
                     /locus_tag="ACG8EF_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFS4947517.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8425..9456)
                     /locus_tag="ACG8EF_002231"
     CDS             complement(8425..9456)
                     /locus_tag="ACG8EF_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4947518.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3284 a         1832 c         1898 g         2856 t
ORIGIN      
        1 aaatattcca acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga
       61 gatggtgttt ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct
      121 ctataatgtc gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa
      181 ataaaaggat gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta
      241 atcgttgtag aagactatga taaagttgat ggaagaaatg cattaaatat ggatgcaaaa
      301 gggctttcac tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag
      361 gtatggcgac atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt
      421 attttagatt tagcgatctt agctgcgcaa ggaagtttat acttccaaga tgcttatcgt
      481 tatgaaaaat tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac
      541 cgaatgacgg tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat
      601 tacgatacac tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt
      661 cttttagatg agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg
      721 agaatttctc actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga
      781 agaaaccgaa aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag
      841 ggaattggca agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt
      901 cggggaagac acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag
      961 tgacctgttt tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag
     1021 attctaataa ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg
     1081 aagctgtact gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa
     1141 aattagcaga gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct
     1201 attacccttc tgaaatcgtt cgagcgattg aaaaaggcta tttattagag attcaagagc
     1261 cgactgtcat ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg
     1321 ggatgttgaa tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca
     1381 ctacaaatcg aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc
     1441 aacatgcgga aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga
     1501 aaactatgat ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg
     1561 atgaaactgc aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt
     1621 gggcaaatac ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt
     1681 ataaattatc cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg
     1741 gattgctact ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg
     1801 agcatacaaa agaaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa
     1861 atgctgaaga aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg
     1921 agggagaaga gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa
     1981 aagaacgcag tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt
     2041 ctcaagaaat gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga
     2101 ataaagaagc gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc
     2161 accgaccagc tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc
     2221 caatagtaga atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga
     2281 cgtaccaaaa aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg
     2341 atttgagaaa ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg
     2401 tacggatcga tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag
     2461 cagcgattgc cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag
     2521 atacagcaga ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag
     2581 acggatttca atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg
     2641 atggtgctgc acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac
     2701 tgttgcttaa tattagtgac ggtcagccaa aagcattgcc ggattataca ggcaaaaaag
     2761 caaaagaaga tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct
     2821 ccgcagctat tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg
     2881 tagatattac tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc
     2941 tatgataaaa taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag
     3001 ttaccggtaa caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa
     3061 ggagtgaaac gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat
     3121 ctatcctgct agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg
     3181 tgaaaaactt aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta
     3241 tcaatctcca tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag
     3301 attcggaacg atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa
     3361 agtgattatg gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc
     3421 gaaaaaatct aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg
     3481 caaagaaccg aataattgga catcgatttt cggcggttct gcttgggagt attctcatga
     3541 aactggacag tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag
     3601 cgagaaattg aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga
     3661 tggatttcgt ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac
     3721 agagaaccct ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt
     3781 gaaacatgtt tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc
     3841 agaagaagga ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga
     3901 tcatatccat atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaaca
     3961 tgcattaagc gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga
     4021 aaaccatgat gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc
     4081 tatgtctgca aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta
     4141 tcaaggacag gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc
     4201 agtagattcg aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc
     4261 attggatatc attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc
     4321 ggaacaatat gctggttttt ctacacatga accttggctg atcactaatc caaataccaa
     4381 aacgatcaat gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa
     4441 catgatccgc attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct
     4501 tcatgaacat ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat
     4561 tatggtaaac ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc
     4621 ctggacatta ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc
     4681 accttatgaa gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta
     4741 gttggacttt gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg
     4801 ctctaggacc accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa
     4861 gggttttcaa acgaggtctg acaggtactt ggacatgctt gatatgcata ccgattgatg
     4921 tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga
     4981 actggtcaaa agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga
     5041 caatttcgaa attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat
     5101 gttcacaacc ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac
     5161 ggatgatcca ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt
     5221 cgcttgtgct gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata
     5281 tgtcattaac tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct
     5341 aaaaccttct ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt
     5401 ataggaaagg caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc
     5461 actccagccg gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact
     5521 aagcttccta ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca
     5581 gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct
     5641 gaaatcaaat cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca
     5701 taaagaaaag cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc
     5761 ggtacaggga tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag
     5821 atcgttaatt gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac
     5881 acttccggta tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt
     5941 actttctgct actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga
     6001 agaaaaggca caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac
     6061 ctcataatga tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc
     6121 gcctttgatc acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat
     6181 ttctgttaac gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg
     6241 cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt
     6301 gatcacagct tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg
     6361 ttttgttttt atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat
     6421 tttgatacca tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc
     6481 caaagatata gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa
     6541 ggcgcttagt ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc
     6601 accagcaaat ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat
     6661 gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct
     6721 catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa
     6781 tagttaggac ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt
     6841 agctgttcgc gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc
     6901 atggacttgc tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat
     6961 cggttgtgga gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga
     7021 atctaataaa aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg
     7081 ccgatctttc aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca
     7141 ccaatcgcca taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg
     7201 tcctttagac agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga
     7261 atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact
     7321 tgttttcaaa ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc
     7381 atatacttga agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt
     7441 ctggttgttt atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg
     7501 atgctcctta gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat
     7561 tggccggtga gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat
     7621 cataggaaat cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg
     7681 agagatgtga aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca
     7741 tttcgtccgc catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt
     7801 accagaagta tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga
     7861 caaagcaggt tgttccatta aataaagaat aggaatctgt tttgattttg ttccatttga
     7921 aatttctctc actcgctttt ttgtctcatc atcaaataaa atgaaagggt cttctaaacg
     7981 atcgataaat acttccattg ttgttgttgg tagatgataa gaagtcatga caggcactgc
     8041 accaaggtaa gacacagaaa cagctagaag gtatgtgtca aaagcggaac tcttataaat
     8101 aataactttg tctccggatt tcactccaag atgagccaat tgataagccc gtttcaacac
     8161 actttcgcag cattttttgt aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg
     8221 ctcatcaaag ataatcgggg tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa
     8281 taagttcaaa ggggaatacg ttatggtctc catcattcac actccattat cttttgttaa
     8341 gtaatataca aattcaagat aatcgtaaca taatccttta tataataaca tagtttattg
     8401 tgaaaaaaag atcatacatt tgttttatat ggaagaaacc cgaacgaatg ccgctaattc
     8461 gtgataagaa aaatcaacca tcttttccca gatatcttga aaagaatggg atgagaggta
     8521 tcgattacca cataaataat ttaccgcgtc agcatcatct aaatatagtt ctgcataata
     8581 acgatatgtt tttctgcata gttctggcga tacgttttta tatactgtaa ttttgctcaa
     8641 aatttggcga atagtccgag gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga
     8701 attttcgtcc gtattttgtg ctgttaactt aaaatagttc tccagaaatg gcatcgcttc
     8761 ttttggtatt ggtactttat tcagtctgtt ttcttttcta ttcagattaa gaatctgctt
     8821 ggtcaaagat acatctttac gtttaagttc tactacttca tggatcttta atccgcaata
     8881 cagaagaatg gcagtaattg caaggttcct cggtctattc ttttcttcaa agctagcttc
     8941 tctgcttgtt tgaaagcctt caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc
     9001 tgcatcgata tatcgcggag ctgctttggt tgtttccggt aaacttgtac ccaaaagaga
     9061 aatctcttct tcaatcagtc ctttttgata caaaaatcgt aaaaaatgat ttaatgaata
     9121 ttgcttcctt gaaatcccgt aatagctatt ttcaaacaat tgctccgttt cttggttcgc
     9181 tgtagtttgg aacgaacgag tatgtcgata gattttagct aagaaattct gataatcttg
     9241 tttttctaaa tggataatca gctgatcatc cacttcttca gaaaatactt ttctcaaata
     9301 atcagtaaac agatgaaaat cttttgcgta ttcatagcga ctgcctaatg aaagggaaga
     9361 acgattttct tctaaaaact gttgaaagac ggaagggagc aagtgtgtac gttgctttaa
     9421 cttttcttgc agttgttttc tagttttgct gtccattaca aattccctcc aatagtgttc
     9481 attttctata ttttaccgca tttcatcaaa tgaagataga cagtttgtct tgatcttagg
     9541 aagcaaattt ttaactaagt actagctaaa agatatgaag taagaagata catttttagt
     9601 tattttatta gtgcagtcat tcttttgatc ttttaattga tttatcctct ttttagctat
     9661 gtggttactt catgagcaat gaaatggatt tggttcgttc taatggcagg aggctctttc
     9721 ttttatttcc gaaaaattag aaaacatttt ttcagaatcg attggctctc catttattca
     9781 ataatggttt gtctaacgat attgttggga taattcttaa agaagtatta gaaatagttg
     9841 gtacttcatt actttacatg aagatttatt
//
LOCUS       DAZUKZ010000098         9825 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000098 DAZUKZ010000000
VERSION     DAZUKZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.100"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..200)
                     /locus_tag="ACG8EF_002232"
     CDS             complement(<1..200)
                     /locus_tag="ACG8EF_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947519.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYF"
     gene            complement(216..830)
                     /locus_tag="ACG8EF_002233"
     CDS             complement(216..830)
                     /locus_tag="ACG8EF_002233"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFS4947520.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(977..1660)
                     /locus_tag="ACG8EF_002234"
     CDS             complement(977..1660)
                     /locus_tag="ACG8EF_002234"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS4947521.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1769..2227)
                     /locus_tag="ACG8EF_002235"
     CDS             complement(1769..2227)
                     /locus_tag="ACG8EF_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFS4947522.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2311..4074)
                     /locus_tag="ACG8EF_002236"
     CDS             complement(2311..4074)
                     /locus_tag="ACG8EF_002236"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFS4947523.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4295..5755)
                     /locus_tag="ACG8EF_002237"
     CDS             complement(4295..5755)
                     /locus_tag="ACG8EF_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947524.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5963..6889)
                     /gene="glsA"
                     /locus_tag="ACG8EF_002238"
     CDS             complement(5963..6889)
                     /gene="glsA"
                     /locus_tag="ACG8EF_002238"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFS4947525.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6912..8339)
                     /locus_tag="ACG8EF_002239"
     CDS             complement(6912..8339)
                     /locus_tag="ACG8EF_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4947526.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8370..9584)
                     /locus_tag="ACG8EF_002240"
     CDS             complement(8370..9584)
                     /locus_tag="ACG8EF_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFS4947527.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2916 a         2041 c         1627 g         3241 t
ORIGIN      
        1 ttaaagtaat taaacaatcc gctaatcgtt tgatctggca tttctatttc cttaccatct
       61 aaagtaacag tagtaacgac aaaatggtct tgcaatggcg gaacttctaa gtactgccct
      121 tgcagaaaat cattcactgt ttcatattct tttttcattt cttttgaatc tgtcgatggt
      181 ttatagctta tttttaacat aactagtgct cctttttatt cgggacgtgc aatcgtgaaa
      241 atttctccta ttgctgcata atcatttcct gccaaacggc ttacagcatt caattttctt
      301 ggatcgatcc ttcctgtctt atcatcatag actgtttcat ttaaatgata tttctgtact
      361 tctagcaaaa ataaatcagc tgttacttta tttttattcc gtatctcgac atgttcatat
      421 aaggatgttt caaaacgaac ttttgcttct gtcaatgctg gtactgaaat gattactgaa
      481 tcagttaaag tcaagtcagt caggtacagt tcgctttcct cattaggcag attggctgct
      541 gttttattta cttcttctac gttgtcctca tcaaggatat ggataacagc ttctcttgtc
      601 tcaagaatat tctttgctgt gtcttttact gcatcttgtt ttctctggat cgccaaggat
      661 aagatgggtc gattcgaact gacgatattg aagtaactga agggtgcgat attcactata
      721 tcttttgctg atttagtcgt tactactgct actggtctag ggatgactga accaatcagc
      781 aacttgtaat tttcacgttc tgtcaattct gaaggactcc gttcaaacat ggtttcgcct
      841 tctttctata aaattcgtta aaaagcagat cttgaagaca aaatgaaaag taatgctttt
      901 ttcatcttct tagaatctgc tttaaactat ctatcaattt ttcttttttg tcaagaatct
      961 gtgccgtgcg tgttttttag ttatttgctt tacttgcggc ttcgacatat cttttagtga
     1021 tcaattgcgg acgggtgata atagatccaa ctacgactga aaaagcacct aattcatatg
     1081 ctttctctac ttgttcagga gagtcagtat gtccttccat aattactggg atatcgactt
     1141 gttctatgat ttgattcaat aaatcaaaat caggtgtctg cgtctttcgt gaatattctg
     1201 tatagcctgc taaagtcgta gacaaaagat caaaaccatc ttcttgcgct tgaagcgctt
     1261 cctcaaaagt cgaaatatct gccatagcta accgaccgtg ttcgtgaatc atttggacaa
     1321 gttcagcagt tgttttttct gtcggacgtt tacgagaagt tgcatccagt gcaatgattt
     1381 ctgcttgcgt ctccaaaagt tcttccactt ctttttctgt cgcagtaata aacacttctg
     1441 acccatcgta atctcttttg ataatcccta ttaccggtaa atcaacgatt cgcttgattt
     1501 cattgatatc ttctgtcgta tttgctcgaa taccaactgc tccgccttct tttgcagcca
     1561 aagccattct ccccataata aaagaactgt gtagcggctc gttttccaaa gcttgacaag
     1621 aaacgacgac tccgcctttg atttgttcca gtgtatccac aatatctcct ccagtaatgt
     1681 tatataaaaa tacagcttca tataaaaatg taaccctttt ctattcagga gacaataatg
     1741 agctatttct ttcagcagac ctttcttact aaaagaaaat tacttcaaat tcttcgtata
     1801 cgaccagctt ctcttctgta aattctatgt tcaacgcttc tttataagca tgtttaaaaa
     1861 actcagcatg gactttccgc caataagcta atgtccgatc cccttctcct tctttccaag
     1921 catgctcttt cgtgacttca ctaaaaggca agatggtcac atttatattt ttgatgatac
     1981 aaactggttc attttttcca ttcaggacca cgtctaattg accaactttt ggtaaaggct
     2041 cattctcttg tttatacaga tcgtaaccac ttgatgtacc agtctttatc ttttttacaa
     2101 ctagttcgct caactcgtct gccatttttt ctgtatcccc aaatgaaaag aaacctaatt
     2161 tttctgattg ataactagga tatttttctt ggaacttttt gacatattct tctacttgct
     2221 gatacataac atccacccct ttgcttttta taatgaaaaa accatccaga tactagtata
     2281 aacaattatc tggatggtga gcataatgaa ttattgaata ttcaagacac gcagactatc
     2341 ttcaacgatt tgttttggtg ttaaatgata acgttcataa agctgcccta caggaacgct
     2401 atccgtgaat tctttctctg caccaaaatt taatacgttc attgaagaat caccataata
     2461 acgactgata ttttcgccaa atcctcctga caaactacca tcttctaatg tgatgactaa
     2521 ttcgtgtgtt tcttttaatt tatctaatgt ttctacgtca ttttctgtga tcgttcctgg
     2581 attgatcaac gttgcttcga ttcctttttt tctaaaagct tttactgttt cttttcctaa
     2641 ttcaagaaaa ccgcctaaag ctaaaattgc tacttttcct ccattattta ctgtttgata
     2701 ggctggaata ctataatcat ttgcgatagc tgtaccatgt tctacaccat gtgtaggaat
     2761 tcggattgct acaggctgcg ctgtttgttc gaacgcccaa tctagcatag agatcatatc
     2821 ctcttttgtt gctggagcaa gatatttgat gttcgggata cttgataagt aaggaatgtc
     2881 aaatgttcct tggtgtgttc ggtcactagc tgagatcgtt ccgccaccga ctaaaataat
     2941 cactggattc tcgttgatcg ctaaatcatg tgataattga tcatatgccc gttgcaagaa
     3001 agtacttgat tggaagacga ctggacgtac tccctgagaa gccaaagcag aagcgaaagt
     3061 gatagagtgc tgttcagcga tccccacgtc aaaataatga tctggatgtt tttcttggaa
     3121 tgttttcagt ccaaaggcgc ctggtatcgc tgcattgact gcggcaatag gagctgtact
     3181 tgtttgcatt ttcttatcca aataatccat gatgatcata ttaaatgttt ctccagaagt
     3241 tacttgcttt gcctccccgg tggacagatc aaatggtaca tgccaatgat atttcatttt
     3301 attatcgatt gccggctgat aaccgcgacc tttttccgta tggacgtgaa gtacaatcgg
     3361 atgatcaata tcttttactt cttcaaataa acggatcacc gtttccagat cgtttccatt
     3421 atccacgtaa cgataatcaa atcccattgc tttaaatagg ttattgctgc ttttaccgtt
     3481 ggtttgacga agctcagcta agtttttata taaactgcca tgattctcat caatagacat
     3541 ctcattatcg tttactaata cgattagatt ggattgtaaa acaccaccat tgttcaatcc
     3601 ttcaaaagct aagccaccac ttagggagcc gtcaccgata aatgctacca catttcctga
     3661 ttctttcttc agatctctcg ctttagctaa acctgttgct aagctgatag atgtagatgt
     3721 gtgccccact gtaaaatagt catgtttact ttcatgttgg ttggaatatc cgctcacttc
     3781 acggtaatgt cctttttcaa aaccattttt tcgaccagtt aaaagcttat gaccataaga
     3841 ctgatgggaa acatcccaaa cgattttatc ctttggtgaa tcaaatacat aatgaaacgc
     3901 aatcgttaat tctactacac ctagattcgg accaacgtgc ccacctacat cgctgtcctt
     3961 ttgcaagatt ttttggcgca tctcttctgc caactgtttc atttcctcaa cagataattt
     4021 tttgacatct gcggggctat tgattgcatc caatacatgt aatactgtac tcataataca
     4081 ttctctcctc cgtactatct tcttttaatg aaaatagatt tgttacactt tatctattct
     4141 actacttaga gttgactcta agtcaatagc attttaaaaa aatttcagag aaagcttgtt
     4201 atataagtag atcttcgcac gaatcaggac tcaaataaaa accttctaca gacaattaaa
     4261 aagctttgta gaaggtctcc taattaaggt ctcatcaaga aatatgtttt tttaataggt
     4321 gaagttcctg agcaatatga tacaaatgga tgtttgcacc ttttaatgag attggataga
     4381 tctttgtctt ttcagtttct aatccatttt gatagctagc agcatcgcac aaaaccactc
     4441 tttttttcgc acatagacat tcatcaggca attcttctaa tgaacgaaat aaaataggat
     4501 ttatttcagc gatgccttct tccgcataca aatgaaattg ggttcgataa aaaaattgtt
     4561 tgactgaact ttctgaaaca acaaaaattt ctatttcctg tctccccgct tgttcactgt
     4621 tgatcaaaga aagacttttg acgaattttc gaatgaaatt ttcattccat cttagttgga
     4681 tttgattttt ctttttccac tcttccaata tttctacaat ttttccgtac agaagttgtt
     4741 ctttttctga tagaagatag atcctttccg gtaaaaatac ttgtccatct gcccacaacg
     4801 tacggacaaa ttgtaaaaag gattgcttga aaataaaatt ttctgcatcg tcgatttcag
     4861 cgatactaat aatctgtaaa atcaattcat tcagtgatag gtcattgaga acaaaatttt
     4921 tataaacaac ttcgaaatct tttcttaaca gctccatgtt ctcattggta taatttctac
     4981 tgttgaacaa agaaaaaata taatagacat cttcttcttg taagtctaga attccttttt
     5041 gctcttccaa tccttgacag actaattgaa ataacggctt cgtttctgcg cttttcttat
     5101 cttcttcttt cagtccaagg ggaaatgcct ttcttccgat tgcgatctct attcctcgta
     5161 tgataaagga cttttcatct tccgaatatc ttcttttaaa aaaatgagtc tctacataat
     5221 caatcaactc gttacaagct tcttctacat acggattttt attttcatag ccttcccaat
     5281 tgacataacg tactaaagct agcaagatgt tccgcgagtc aaattctggg ataatgattt
     5341 ctttattttt caaataatca ttttctttaa aaaaatccaa tacgattttt ttgattgtat
     5401 atactttgct caaagaaata aattcaagat cagccagttc tgtactcgtg aatataccat
     5461 taaaataata acttaaaaac tttaaaaaat aagattgacc gtacagaaac tgtgtgagct
     5521 cagaaagagc aataccatgt ttgaatacga gtttatactt atgcttctct cttacttgtt
     5581 cttttataca atattctaga cactcggcta gactttctag atcgtgaagg atcgttggtg
     5641 tagtcaccct tagagaaata gctgtttgtt cgatgtctat ttcttggaat tcaaataatt
     5701 gttcacacag atacactttt ctaaatatat ttcgctcgat gtaattttcc agcattcctg
     5761 atacacgcct ccagtttttc ttatgatttt atttaatcat atctgtaaat cgtatttttg
     5821 tttccttttt atttagaaaa gccctttcaa aaaccaataa aaaaagaaac tgggacaaaa
     5881 gtcaaacaag ctcacaattt cagcctcatt gtaggtagct ttgagccgtt tggactttta
     5941 ccccagcctc tttcaagaat ggttattcaa agatgtcagc gtctagttct ttgactacat
     6001 ctttcaacaa ttggatacca gctgcgctgt taccaaatgg atctagtgcc gggctgaaga
     6061 cacctatgcc gtaacgattt ggcacagctg ccatcaaccc gccgcctaca ccgctttttg
     6121 caggtacacc aacatggacg gaaaactctc ctgattcatc atacaaacca gcagttgtca
     6181 taattgattt gacgattgta gcactttctt cagtaatgat ttgtttgcca ttccaaggag
     6241 caattccttt atttgccagc acagcagcta attttgctat gcctgtagcg gtgacattga
     6301 gggagcattg tctgaaataa gcatcgagta tctccggtac gtctccttcg atcatttgat
     6361 tacctttcat atagtaagca agagagcgat tgatatcacc tgttcttgat tcagaatgat
     6421 agatttcttc attcaatgtg atattaggat catcacagat ctctttcata aatgacaaga
     6481 ttcgctcaaa tttttcatct gcatctttac cggcaatcaa cgaggttgtt gcaatcgctc
     6541 ctgcattaac aaaaggattc attggatgtt tgcgatgatt gatttccata ttcatgatag
     6601 aattgaaggc aaaaccggtt ggttccgtgt tgattgtttg aaagactttt tctattccgt
     6661 tgtctttgat tgctaataac aatacgggaa cttttgacat gctttcgatg gcaaagcgca
     6721 catcggcgtc tcctccctca atcagttgtt ttgtatccaa atcataaatc gccataccta
     6781 attgtttggg atccacttca gctaatgctg gaatgtaatc cgccacttgt cctgtttgat
     6841 aatagtggaa attttttcca atcaattgtt ctaattcttt tacttccatt tgaataacca
     6901 gctttcattt tttatttttc tgcatgctgg aatttatgac ggcatgcgta gatcgtaaat
     6961 ggaataacta agacgataag ataagaaaca atcagaatcg tttcataaat cccatgctgt
     7021 ccagctgcga tactagcagg tggaacaaat gagatacaaa aagcaaaaag tgaagtcaaa
     7081 agacctaaac aagcaacgat tacttttaca acttttccgc ctggaacctg ataggctctt
     7141 tcaagactgt catctttaaa aaccagcttc aaatagcccg cgaaaaacat aaagtacaac
     7201 atcaggtaga ttacaacagt caatgacatc gctgcaagaa atgaaagatt attccccccg
     7261 ccgcttaatg taaagacggc tgcccagatt gttgcaacga tcccatttgc gatcatcaaa
     7321 tgagcaggaa caccgtgtgt gttttctttt gcaaaatatt caggaagtaa tccttcttct
     7381 gctgctacgt gaagtgcttt tgaaggagag gttacccaag aagcaatttc tccgatgaca
     7441 cctaaacaaa caagaatcgc aagaattttc gcaatccatg caagatttgg atcaatgtgt
     7501 cttaatagga attctaacga ttgaatcacc cctgtattaa gagacaaacc atgttgaggt
     7561 actgtagtag cgacggtcaa tccgccaaaa gtatctaaaa tgattgccag aataaccaat
     7621 acaaacattg ctaatggata atccttttta gggttttcca tttcatttgc atgactggca
     7681 gatgtttcta cgcccatata tgctaaaata aaggaaacaa aaacaaccag cgtaggcaat
     7741 ttcgaaaaat caggaatcaa tgttttcata gacagtatcg tttgtagtgg agcgcctgaa
     7801 gcgacataat ggatccctaa gccaaacaag actaatgctg gcaacaagat accagctaca
     7861 aaaccgatcc gagcaatcaa tgaagtgtat ttggttcctt ttaactgtga gagtaaaata
     7921 gcccagaaaa taatcaatag gattgccaat ttcagatatt tattgttttg tatagcacca
     7981 atacccgttg cataggataa tgcaccagtt aagaaataca gcatggtcaa gtagccaact
     8041 gttatctcaa accattggaa aaagattgcg gcaaaaccga atcgttttcc caatgtgcga
     8101 ctgacccaag tatagacacc gcctttttcc catcctttca ccgttgccat ctctgctgcg
     8161 cacaatgcca cagggataaa ccatagcaaa ccgcctaata acaagaaaaa tactaagctg
     8221 aagcctgagg tcgcgaatgt gggatattca tataatgaaa cgaccattga gatggtcata
     8281 gccaaaaagc caaagaacgt taattttttt gcaggaattt tcgttttgct ttgttgcatg
     8341 aatcttcctc ttttctattt gaatttgttt caattgagtt tgttgccaaa gaagaaaaag
     8401 tccaatcctt cttcttgttc ttcttttgta gtagtgatcg ttgtaaagtg catcgccgat
     8461 tttaaaatca ctgttgtcag cacataggca tacacggcag taatgatgac accaatcact
     8521 tgaatcccta agaagtacca gctgcttgga ttcgttagta aatggctgtt agatgcaaag
     8581 atccctatca gaatcgtacc tgtgaatccg cccatgccat gaacacccca cacatcaagt
     8641 gcatcatccc attgtttctt tttacgaaaa tcaacagcag catgacagac gatacccgca
     8701 atgatcccaa tcagcatggc actttttgct tctacataac cggcacaagg cgtaatggct
     8761 gccaatccag caacggatcc tgttagtacg tcgacaaaat caaagccatt tccttttact
     8821 tttgcaatga tcagccaagt aatcatcgca aatgcaagag cgatgaatgt tgaaccaaat
     8881 gcattgactg cttgttcatc tgctcgaagt gcaccaccgg aattaaaacc gaaccagcca
     8941 aaccaaagca gtcctgtacc gatagcagct gccataagac tgctatgctg acttctttct
     9001 cctaaaggaa tctttctttg accaagtgtg aggatacatg ctaaagagcc gaatccagct
     9061 gtcgtgtgga tgactgctcc accagcgaaa tctctaaatc ccatctgatc caaaaatccg
     9121 ccaccccata tccagtggca aacagggaaa taaatcagca gattccaaaa aataacaagt
     9181 ataatatagc cttttaaatt caatctccct gcaaaagctc ctgtcatcaa aggaacggta
     9241 atcacgataa acatcagttg gtaaagaaag aacattaaaa atggaatcgt tgcaccatga
     9301 aacatgttgg ggaagcttgt aacatgtctc atcaaaaaga aatctgccgg gtttccaata
     9361 acaccgccga tatcccgtcc aaaggccaat ccgaatccac caaataccca cataatcgtt
     9421 gttatcccaa cggagagaaa agattgagac atgatcgtta gtatattatt cttccgtgct
     9481 aatccgccgt aaaaaaaagc tagaccagga gtcatcaggc aaaccattgc tgtacataaa
     9541 atcatgaatg ctgtatctcc tgtattgata ggtaatgtat tcattttttt cgcctccttt
     9601 ctacgattac gagtataaaa gaaggtgtag ttgttttttt cttctgtttt tttgaattaa
     9661 tacttgcatt tataaaagac agacaattaa taaaagcttg atgtgttgtg ctctttttcg
     9721 tttttcacac aaataaagac aaaaaataaa cggtcttctt tttatagaaa accgtttatt
     9781 ttcgtctttt tttattttta aagatatttt ttcataagga ataaa
//
LOCUS       DAZUKZ010000099         9824 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000099 DAZUKZ010000000
VERSION     DAZUKZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.101"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..751)
                     /gene="atpB"
                     /locus_tag="ACG8EF_002241"
     CDS             complement(32..751)
                     /gene="atpB"
                     /locus_tag="ACG8EF_002241"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFS4947528.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(1053..1517)
                     /gene="smpB"
                     /locus_tag="ACG8EF_002242"
     CDS             complement(1053..1517)
                     /gene="smpB"
                     /locus_tag="ACG8EF_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFS4947529.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            complement(1539..3896)
                     /gene="rnr"
                     /locus_tag="ACG8EF_002243"
     CDS             complement(1539..3896)
                     /gene="rnr"
                     /locus_tag="ACG8EF_002243"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFS4947530.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            complement(3899..4654)
                     /locus_tag="ACG8EF_002244"
     CDS             complement(3899..4654)
                     /locus_tag="ACG8EF_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4947531.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(4757..4993)
                     /gene="secG"
                     /locus_tag="ACG8EF_002245"
     CDS             complement(4757..4993)
                     /gene="secG"
                     /locus_tag="ACG8EF_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFS4947532.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(5111..5389)
                     /locus_tag="ACG8EF_002246"
     CDS             complement(5111..5389)
                     /locus_tag="ACG8EF_002246"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4947533.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(5502..6470)
                     /locus_tag="ACG8EF_002247"
     CDS             complement(5502..6470)
                     /locus_tag="ACG8EF_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4947534.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      6685..6729
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            6753..7727
                     /locus_tag="ACG8EF_002248"
     CDS             6753..7727
                     /locus_tag="ACG8EF_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFS4947535.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            7732..8307
                     /locus_tag="ACG8EF_002249"
     CDS             7732..8307
                     /locus_tag="ACG8EF_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFS4947536.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(8374..9513)
                     /locus_tag="ACG8EF_002250"
     CDS             complement(8374..9513)
                     /locus_tag="ACG8EF_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFS4947537.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         2720 a         2089 c         1724 g         3291 t
ORIGIN      
        1 gataaatagt agtttgtttt taaaacgaat attattcttc cacttcaatc ttatggctca
       61 tataaaccat agataaagta acgaagacaa atgcttggat gcttccgata aacaaggaga
      121 atgcgatcca gaccatctct aatggaattg ctaacggcaa tgagaaccag cctaagctta
      181 ttaccatttt tgcaatcaag gttagcaaaa cttcccccgc ataaatattc ccataaagac
      241 gtaaagcaag tgttaataag tttgtaaatt cttcaatcag tttcatcgga aacataaaac
      301 tatatggttt caagaacgaa tttacgaaat atcctttgaa gcctaacttt ttcaccccaa
      361 ataagtgagt aagtaagatc attataaatg ccaaagttaa agtaacaaac gggtctgctg
      421 tcgggctttt ccaaagtgtt gtttcgttag gtaaaacgat ttttgttact aaaccgatgt
      481 tgtttgcaac aaaaacaaat aaaaacaatg taaatgccaa caaatggaaa ttgctcaatt
      541 cttttctagg cagattatct gttacgattc ctcgactaaa atcaatgacc cattctatcg
      601 cattttgttt gcctgttggt ttcaacttaa gatttcttgt aaaaaagtaa gctaagaaga
      661 aaacaatcaa acaggtcagt aacaccatta tacaaactgt accatcaaac caaattggtc
      721 caatgtggaa cgttagcgag cgttcatcca atccatttca cctcttttcc ccgaaattca
      781 cataaaatca attcatgtgc gtatctggtt ttttccaatc ggtttctaac cacacggtat
      841 cataccacca tgaaacagaa aattcaaaac attttcatga aaattagaca cttttttatt
      901 tcagtcaaaa acgaagcgaa tttttcttta tacacggtgc tcctgatcta agaaaaaacc
      961 tatgataatc atgttttatc agattaaatg ctaacaaatt ttatcattaa gtaaaagcaa
     1021 aagcaccgaa tatgcgaaaa aggataatga aattatctag agaaattttt taatgttcga
     1081 tcaatttgtc gatcgatatc ttttcgtttc aaatcttccc gtttatcata agattttttc
     1141 cctttcgcca gaccaatcaa taccttggca tagccgttac gaatatagac ttttaaagga
     1201 acaacagtga ttccagtatt tttcaattca ttttcaagcc ggatgatttg ttttttgtgt
     1261 aataataact ttctcgtgcg caacggatca tgattaaaaa tatttccttg ttcataagga
     1321 ctgatatgaa cattatgcaa gaaagcttct ccgttgcgga cgcgaataaa tccatctttt
     1381 agattgatcc ggctgtttcg tatcgacttg atctcggttc cttgcaagac catccctgct
     1441 tccatcgtgt cgatgatcga ataatcatgg cgagctttct tgttttgtgc aattaatttt
     1501 ccctcgcctt ttggcatagc tgtttcctcc aactcatttt attttttctt tttcttcttt
     1561 ttaacacctt tgtaaaatgg cttcttccct tttttcttac cgaacgtttg cttactatcg
     1621 ctatatcgtt tgttcttctt gccatttctt tctgggcgtt ttcctgcttg ttttccttcg
     1681 cgttttcctg attttccatt tcgtgttttt ggccgttcca acgctgtaac ctcttctgcg
     1741 gaaacaagtt caaaatctac tgctcgttca tctggatcag ctttgactac ttttactcgc
     1801 actttctgtc cgattttaaa agtcagacca gttctttcac ctacaagagc taaatgattt
     1861 tcaacaaaat ggaagtagtc ctgtttcaat tcattgatat ggatcaatcc ctctactgta
     1921 ttcggcagtt cgataaacag gccgaatttc acaacagagc tgatgatccc atcgaattct
     1981 tcccccactt tgtccatcat gaattctgct ttcttcatgg aatctacttc acgttctgtt
     2041 tctactgcac gacgttccat cttagaacta tgatctgcaa tttcaggaag ttccgtagcc
     2101 cacgaacttt gattgctttc acttttatct tcactgtaag aacggatcag acgatggacg
     2161 attagatcgg gatagcgtcg aataggagat gtaaagtgtg tgtagtaatc tgccgctaag
     2221 ccataatgcc caaaattgtc ttctgaatag cgtgcttgtt gcatgcttct tagcaacata
     2281 gtattgataa ctgcagcttc tggtttgtct tctactcctt cgatcacatg ctgtaaatct
     2341 tttggtgtga tcgttccttt cgttcctcgg accagaatgc ccaaagctga agcaaaatca
     2401 aagaagcgct gcattttttc ttcttttggc tgttcatgga ttcggtaaat aaacggcaga
     2461 tttaatttat tataatgttc agctaccgtt tcattggccg caagcatgaa agattcgatc
     2521 aagcgttcac caattcctct gcttcttagc tggatatcat ttgggtgtcc ttcagcatca
     2581 acaaggattt ttgcttcgcg atcctcaaaa gaaatcgcgc cacgtttgat tcgcatcgtt
     2641 tcaaggattc gatgcaattc acccatttct tggaacatag gtacgagctg agcatagcgt
     2701 tgcatcgttt cttcgtcttg atcttctaag atttgattga cagctgtgta ggtcatccgt
     2761 tctgttgttt ggatcacact tgggaaaata tcatgagaga ctacttgtcc atttggatca
     2821 atctccattt cgcaactcat ggtcagcctt ggaacatggg gattcagtga acagatacca
     2881 ttcgataaac gttgcgggat catcggaatc actcgatcag tcaaatatac acttgtccct
     2941 ctttcatatg cttctctgtc caattgactg ccttgtgtca catagtaaga aacatctgca
     3001 atgtgtactc caagaaaata attaccgtta tctaattttt taaccgtaac tgcatcatct
     3061 agatcttttg catcttctcc atcaatcgta acaatgagct gatttcttaa atctctacgt
     3121 ccagttatat ccttttcgct gatttgatct ggaacttcat ctgcttcaga caatacatca
     3181 tcaggaaatt tcgtagggat accattagcc acgacaattg agaggatatc catgccaggg
     3241 tcattcttat gaccaatcac ttgcttgatt atcccttcta aactagtcgc ataccctttt
     3301 tcagggtaat gggtcagttc gatcagcaca atacttccgt caactggacg aactccctct
     3361 gctgctgcat atactttaaa ttggttgagc tttctatctt tcgggataac aacgccgtaa
     3421 agatccgttt cttgtatttc ttcttctgaa taagcaacga attcccctgc taactgagta
     3481 gtcgcccggt ctcgaatctc gactactttc ccctctgctc cgcgatccat atctgtattt
     3541 gctcgcttca tgatatcgat tgctacggtg tctccatcca ttgcaaaacc agtcgcttct
     3601 tttgggatat agatgtcatc ttcttcggga tcaatcgtta caaatccaaa tcctcgttca
     3661 tttgctcgaa atgttccttc gatcaagatt ggttttaaag gaagtttcac ttttcctttt
     3721 ttattgaaaa ctatactctg ttctctttcc atttgagcaa tcgtttgtac tagtaattta
     3781 aaatcatcgc ttttttggaa ccctaaacct tcagcgattt cttccataga aaaactgcgt
     3841 ttgcgactgc tttccatgaa aaagagaatt ttctctttta tcgtttcttt tgtcatattt
     3901 attcctcatt ccaaggtaat ttttctaaaa attccaagac gtctttttct aattcgcgtc
     3961 ttgctgtacc aactgtaatc acatgaccac tttcaggata ccatcgaagt gtcactcgct
     4021 gacggctcaa cttgcgtgca gtttcaaata cgccattagg gtcgatcatt tcatctttcc
     4081 cagcttgagc taaaaatatc ggctgttgga tatctgacaa cctactttct acgattgctg
     4141 cctgatcttg tatatccatt aattgttgtt cagctaacga gcggtaagca actgcttttt
     4201 cattgatttc tttctctgat ttacctgctg tcttcagcac ttgacgtacg tatttttcaa
     4261 agttttcagg cacgtttgtt tttacaggag acaaaggcga acagaaaaaa ccgccgccaa
     4321 tcactgattc ttcttctagt gctcgtacag caaagatgcc gcccattgat aatcctaata
     4381 cggcaatatc agaaaactgc tcagactgta aaaaatggat ggctttttta gtgtctgccc
     4441 accagctttc tgctttttct tgcaaaatat catatggctc cattgttccg tgtcctttaa
     4501 aaagcggcgc ataaactgta taatctgctt tttccaaaaa tcgtgcgagc attcgaacat
     4561 cgtttggact tcctgagtaa gcgtgaagca gcaaaaccgc tcgctttcca tgttttgcat
     4621 aaaggggttt tggtaattgt ctcactcgtc tcacctcttt cgcctcattt taccatagtt
     4681 tctttcataa cttcttctta agcgttctct ttgcaataaa aaaagccgcc cgccgttttg
     4741 cgagaggctt attcaactac ttcgaagata aatatgctag gacaaataat aagatcatcc
     4801 agatagctcc cataactgca gttgatcgct gcatgacagc ttcaaagccg cgagcttttt
     4861 gtttgccgaa tagttgatcc gcaccgccgg taaatgcact cgctgcacta ttttgcttgc
     4921 ttggctgcat cataatcgca atgaccataa caatcgataa aacgatcacg attcccaaaa
     4981 ttaaattata catatcgcag cctccttccc aaaagatatc gtctctttaa ctttatcata
     5041 gataaaagtc cgtgacaact ctatataaat ggaataacag ctgaaagttc aaaactttca
     5101 gctgttattc ttatgactga cgtaagacgt cttgatattc tggtttcttt tcaaactctt
     5161 tttttgcata agggcataaa ggaatgatct ttttcccgtc tcgtcttgct aattctactc
     5221 cagtatacac tagcttttca gctaactttt gaccacgata tgctggatct acaaatgtat
     5281 ggtcaatgat catgatgtct gggcccgcat ctgaccatgt catttctccg atctcttgtt
     5341 gttcatcatt gaataaagca agacgatttt tttcttcttt gatttccata tgccgcactc
     5401 ctcaaataac tgatattttt ttattcctac cttattttag cagtctcctg atctataaac
     5461 aattatttat cctcgaatta cttacaaaaa agaagagaaa attaattcat cgttattctt
     5521 tgttcaataa atgaaaaaat cactgtccaa taggttttcg gatctttcat cgatgcttcc
     5581 aagtgttctg cttctggaac aagcagcagt gctttatccc ctgccgtagc attttgaatc
     5641 gtgtacgctg cttctgtagg gacaaaatga tctctttcac catgaatgac caataaaggc
     5701 agatggttgc tgccaagctg tctcgtagct gatgcttgct ttaaggagta accggcgtat
     5761 ttctgggcat aatgattcgc atatctcatg accatttttt taggaaagac agtgatttta
     5821 cttaacatat atcggaattc tgcataaaca gaaacatatc cgctgtctaa aataaatcct
     5881 ttgaccgctg atggaagttt ttcaccactt gccatcatga tcgtagaagc tcccattgat
     5941 ccgccgtgta aaataatgga cgcattcggc tgttcatcta agatgagttg gatccaggca
     6001 attaaatcta gccgatctag ccatcccatt ccgatgattt ctccttcact ttctccgtgc
     6061 gcacgtaaat caggtacaag aacgttgaat cctttctctg catatctttt acctatatga
     6121 gacatgtctc ttttccctgt gcttcggtaa tcatgtacgc aaatgaccca tttattctgc
     6181 ttatttggat ggatgaacat ctgaccagct agtttcaacc cgtcataact tttgattgtt
     6241 ttgggtgttc cctgctccca aaagctttca cactcttctt ctaatttttg tcgttttttt
     6301 tctactgacg tgactgcagc agggtccatc aaacgatgac cagtgtcttc ataccagtca
     6361 tatcctctct gtactcccgc tcttactaat ttctttgcat aaaaataaga aagaaaatgg
     6421 taactgatcc ctataattat cagaataaga atgaaataaa aaataaccat tgtacacccg
     6481 ccgctttctg ccaaactata atctaactat tatccaatgt accgatccat aggattcttt
     6541 attttcggca cacgtaaatt ccatttcttt ttcattgtat catttactta aagttctcct
     6601 caatagattt acttgtaaga gagcatttac attcgcaata aaagccagta tactaatttc
     6661 gtgcattata atcaagacgc tcgtcttgga ctggttcgga aacttcccag aataaaaaac
     6721 taagtatctc taaagaaagg agattggaaa taatgcgaaa agtaattatt gattgcgatc
     6781 cagggatcga cgacacttta gctttatcat tagctgtcaa atcccctgat atcgaagtag
     6841 ttgctattac cgtagtttgt gggaatgtcc ctgcagatat aggtacgcag aatgtgctga
     6901 aatgcctgga acgctgtgat cgtttagaca ttcctgttta tcaaggaatg gaaaaacctt
     6961 tgaagcaacc tttcatcagt gcacaagaca ctcatggtat ggatggttta ggggaaacaa
     7021 atttcccgat gatcttgaga aaacaggcag aacctctcca tgccgttgat tttttggctg
     7081 attatttcaa agaaaagaca aatacatcag tcattgcttt gggtcctttg accaatatcg
     7141 cttttgcttt gaaagtcaac ccaaatattg ggaaacatat ggatcgtttt gtctcgatgg
     7201 gtggaactta caaaagccat ggtaactgtt cgccagttgc tgaatacaat tactggtgtg
     7261 atcctgaagc agcttcttat gtattcgaga atctcaaaca aacgatagaa atggttgggt
     7321 tagatgtgac aagagaaatc gtcttgacac cgactatcct cgaatactgc tgtcaaatga
     7381 atcctgaaga gggagagtat ctaaaagcga tcactcgttt ttattttgac ttccactgga
     7441 aacaagaaag aattttagga tgtgtcatca atgatccgtt agctgttgct tattttatcg
     7501 agggggccat ctgtgaaggt ttcaaaagct tcactgctgt ggaaactcaa ggaataagtc
     7561 gtggacaaac attggttgat cgttatgaat tttggcagaa acctgctaac agtaaaatta
     7621 tgacaaaagt agatactcgt ctttttttcc gaaagttttt aaccgttctt ttaaatgcac
     7681 aagaagaaac gattatgaaa gatttggaaa ggttaaagat gggctaacaa aatgaataaa
     7741 aacaaaatgg caactcgcgt acttacagtg atggctttgt gtattgggat caattatatt
     7801 ggaggaacga ttgctttatg gcttcgtttg ccaatctatc tcgattctat cggaacgatt
     7861 tttgctggtg cattgcttgg gcctgtacct ggaatgctga caggattgtc cagcggtatt
     7921 ttaagtggag tgactacaga tattttttct ctgtattatt cgccaatcca aattctcaca
     7981 ggcttgcttg ctggattgat tttgccaaga tatctcggca aagataaaaa cagcttgaaa
     8041 ctgttattac tcgctttgat tttgtcggta cctggaacga tcgtttcttc gatcattacc
     8101 gttcagttat ttggcgggat cacctcctca ggatccagta tgatcgttca gctattacat
     8161 gggttaggtt taagtcagac aacaagtgtg atcttggtac aggcaggtac agactacctt
     8221 gatcgtttgt tgtctatcgt cgttgtttca ttagtcatca ctatgttgcc acaacgcttg
     8281 atatattcaa aaagaaaaat aggctgaata aacagagctg gattagcaaa ataatgctaa
     8341 tctggctctg ttttttcatt tattaaaaaa atcttacgct aagtaggata aaataattcc
     8401 ttgatatttt tcaatcatat ccaaataatt atcgatttca acatactcat ctacttgatg
     8461 aggcaaagta acgacaccag gcccaaagac gacaaagtcg aaggaatggt tagctttcgt
     8521 aaattcggct gcatccgttg ttgctgccgc tccaactaaa ggtaatggct ggctgaattg
     8581 ttgctgaatg caatgaatca aaggagaatc tggatcagct ttgacaggta ttttgttgta
     8641 atcgatcatc aattccaaat gatagtctgt ctcttgattc aattcattga cgattgattg
     8701 cagcaatgca atgattttat catttgggta ttctggaatc gatcgaatgt tcccttggag
     8761 ctgcgcatgg cttgggatgc tgttgacttg attcccgcca ctgatcaatg taacattatg
     8821 gattgttttt cctaggacag gattctcgat tgtttcagcg aggtgattca tttcagcatt
     8881 tgctttcgta atgaattcat ttaggtgatt gattgcatta taaccttggt ctggcataga
     8941 gctatgcgct tcttttccgt gcgaggtgac tgtatagttg attgatccca tgtgcgtata
     9001 catcaaactg taattagttg gctctccgat gatcaaggca tctaagtcat ctacatagcc
     9061 tgctttcgtg agctgttccc cacctagttc acccacttct tctccgacag tggctaataa
     9121 tttgactgtt ccgttaaatg gttttcctga ctctttcagt tcgatcatgg caatcaccat
     9181 tgctgccaag ccactcttca tatctgtagc accacgtccg tacaaacggt tcccatggat
     9241 ttctgctgca aagggtgcat aggtccaaga cgattcatca ccagccgcca cgacatccat
     9301 atgcccagat aggcctaaca cttttcctga ttggcccttt tggtaacgag caatcaaatt
     9361 atctctacca tctctgtaag aaacaatctc tcctgttatg tcatgctttg ccaaaagctt
     9421 attcaaatag gctgctactt caccttcgtt tccattcact gatttaattc ggatgatctc
     9481 ttgtaaaatc gcgatttttt cttctttttt catctaatcc tctcccttgc catttctttt
     9541 tattgtattt gagtgtgctc gaattttcaa ataggagcaa ccaaagtaaa aatgaagaaa
     9601 gagagccact ttatgaaaaa tatgctgcta cttccctcct attttattta tacgagagga
     9661 cttgctcata gattcctatt ctactgttct atccttaaaa gctcttatat gatgctagtt
     9721 attgttagct ttcctttttc aaggatgatc ttttcttgta ccattctttg taaacatcat
     9781 atacagaagc tagttgcaaa aaaaactctc tacctgctga tatt
//
LOCUS       DAZUKZ010000100         9579 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000100 DAZUKZ010000000
VERSION     DAZUKZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.102"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..523
                     /locus_tag="ACG8EF_002251"
     CDS             14..523
                     /locus_tag="ACG8EF_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4947538.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            616..1778
                     /locus_tag="ACG8EF_002252"
     CDS             join(616..892,892..1778)
                     /locus_tag="ACG8EF_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4947539.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2058..3038
                     /locus_tag="ACG8EF_002253"
     CDS             2058..3038
                     /locus_tag="ACG8EF_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4947540.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3160..4578
                     /locus_tag="ACG8EF_002254"
     CDS             3160..4578
                     /locus_tag="ACG8EF_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS4947541.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4649..6118
                     /locus_tag="ACG8EF_002255"
     CDS             4649..6118
                     /locus_tag="ACG8EF_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4947542.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6128..6595
                     /locus_tag="ACG8EF_002256"
     CDS             6128..6595
                     /locus_tag="ACG8EF_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4947543.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6631..7503
                     /gene="scrK"
                     /locus_tag="ACG8EF_002257"
     CDS             6631..7503
                     /gene="scrK"
                     /locus_tag="ACG8EF_002257"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS4947544.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7584..>9579
                     /locus_tag="ACG8EF_002258"
     CDS             7584..>9579
                     /locus_tag="ACG8EF_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4947545.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNAL"
BASE COUNT         3320 a         1399 c         1933 g         2927 t
ORIGIN      
        1 gggctatact atattggtac aaggaataga aagaggtgtt tatgtggttg ataaaattca
       61 gtttaatcaa gttatagaag aattcatcaa agtaaataat ttaatgaccc atattcaaaa
      121 acgaccaatc agaataacta atgatctaaa actcagtacg agtatgattc atttgattga
      181 tgtgattgga aattatcctg aaactagtat tacggaatta gctgatcgat taggcgttac
      241 caaaggagct gtatcccaac agattcccac tttagagaaa atgaatctta taagcatttc
      301 tcaaaaaaaa ggaaataaga aaactaaact tgtatcgtta acaaaatatg gtcgaaaggt
      361 gtacgaatca cataattatt tacatgaaga gctttatagc tcaataaatg aagcgttaga
      421 atcattctca ccagaccaaa tttcaaccat taaacagatt ctagaaaaag tatcttatac
      481 aatcaatgaa tatcaagtaa gtctgacaag aaaggaagat tgacatgaaa aaaatgattt
      541 ttgaatcggc tacactagac tagacagaaa aaataaggtg tgtaaaataa atgaaaacac
      601 actggaggaa tttttatgtc aagacgtcaa cgtagaacct attcaaaaga attcaaacaa
      661 caagtcgtcg atctttatct cgcaggaaag ccccgagctg aaatcattcg agattatgag
      721 ctgactacct cggcctttga taagtgggtc aagcaagcgc aaactactgg ctcgtttcag
      781 gaaaaagata acttgatccc tgaacaagta gaactaattg cattacgaaa gaaaaataaa
      841 cagcttgaaa tggagaatga cattttaaag caagcggcgc tgatattggg acgaaaagac
      901 aagtaattga taccaataag cacaaatact caatatcagc gatgtgtaaa gtgttaaaca
      961 tctcttgcca aacctattat tatcaagcca aaccagttga agatgaatca gaactggaag
     1021 aactcgttca agaagaattt attcgcaatc gaaaagctta tggtaccagg aagctgaagc
     1081 agtgcttggc caatctaggt cttcaagtga gtcaacgcag aatcggccgt atgatgaaac
     1141 agcgtggctt gcgttcaaca tacacattgg ctcatttcaa attccattct tctacttgta
     1201 acgaagccaa gaccgccaat attctagacg gaaccttcct acaggaaaag tcattagaag
     1261 cgattgtaac cgaccttacc tatgttcgtg ttgggaagaa atggcagtat atctgcctga
     1321 ttcttgattt gtttaatcga gaaatcatcg gctattcatg cggggagaag aaagatgcga
     1381 cgttagtcaa ggaagctttt gcaaagattc cgtactcgtt gaataatgta caactttttc
     1441 acacggatcg tggaaaagaa ttcgataacc agacgattga cgaaatttta tgggccttta
     1501 atatcacgcg ttccttaagc aaaaaaggct gcccctatga taacgccgtt gcagaatcca
     1561 cctataaatc tgtcaaagta gaattcgttc atcaataccg ctttgagaca ttggctaatt
     1621 tgcgattaga gctatttgat tacgtccatt ggtggaatac catccggctg cacggcacgt
     1681 taggctatga gacaccgatc agttttcgac agcagagatt ggcgcagcga atccttgata
     1741 atgagctcgg atgtgatacc ggtggcgagg caccttaatt atttgaatga gcttctgcct
     1801 tagagaatca gattcgagga ggctcgttgt caagggcagt cggaacacca gcagcaagct
     1861 tacgcaatac cttataattt ttgtcaaaaa aagtgttgcc attccacata tatctttaaa
     1921 cagctattgc cagataaaaa taaatgatat tatatatata aatacttatc cgaaaagggg
     1981 aatgagagag tcaaggttct tggaatgtcg aatagtcgag taggtagatt ttctatctac
     2041 aaccttgttg tttaaaaatg aaaaaaattg gaattagaga agttgcgaaa gaggcaaatg
     2101 tttctcctac gactgtgtcg agggtattga atgatcgtgg atatttgagt gaggaaacaa
     2161 aaagaaaagt ttttgatgcc atgaaaaaat tagaatacta tcctaatgaa ctggcgagag
     2221 cactttttaa aaacgaaaca tataccgtag gtttgatttt tcctacaatt actaatccat
     2281 ttcacgctga gttaattcaa gatatcgagt tcgagctttc cagcagggga tataaagtgt
     2341 tgctgtgcaa tagcttaaac aatccaacaa aagaaaaaga gtacttgacg atgttgagaa
     2401 agaatcaggt tgatggaatt atagtaggaa cgcacaataa agatattgcg gattatgata
     2461 ttccagggct acctattgta gcgattgata gaagtcttgg tgaaaataca gttacagtat
     2521 cttgtgataa ttacaggggt ggggaattag ctactcaact acttgtaaat catggctgtc
     2581 agaatatact gtgtattcgg ggagatagta agataaagct acccgcgaat agtaggactt
     2641 tagcatataa agatgttttg aataaattca tgctggaacc gttagtccta gaagtaccat
     2701 ttgtaaaaga aatcaatgaa aaaaatgaaa taattaagac atatctggaa actcatccaa
     2761 atattgacgg ggtttttgca ggagatgatt tattggcttc tttggcgatt aacaacttag
     2821 gaagttcggg caaaagagtt ccaaaagagg ttaaagtgat tggatttgat ggagctcaac
     2881 aaacattgat ttataatcca gaattatcaa ctattcaaca gcctatagat caaatatcta
     2941 aagtagcagt aaaaaaatta ttgaatatga ttaagggcga aaaggagacg gatgaacttt
     3001 cacttccagt aaagctcata aaaaaaaata cagcataagt taaatcggcg aaaaattcct
     3061 tcgtcgattt aaaaatattt ttatgaaacg ggttgacata tgaaacgggt tgacatatat
     3121 tagttgtgaa gataaaaacc ttttcactgg aggaagaaaa tggagaaatt attaattaaa
     3181 agcaataatc atcaacttta ctaccaacct aaggatgttt gggtcggaga tgttatgcct
     3241 tacgggaaag aaggaaaatt ctttatttat catcaacgtg atactagaaa tccagttcct
     3301 tttggagaac cgtttggctg ggctttagca actacgaaag attttgttca ttttgaagac
     3361 tttggggaat ctcttttaag aggggatgac aatgagagag accagtttat ctacgctggt
     3421 tctgtatttg aggcaaatgg aaaggttcac gcattttata cagggtataa tcgtgaattc
     3481 ttagcgcagg gaaagacatc tcaagtgttg cttcatgctg agagtgatga ttttgttcat
     3541 tgggaaaaat cagaaacagc attggagtta gaaccacaag aagggtatga tattcgaaac
     3601 tggagagatc catttgtcgt ttgggatgat gaaaaagaag aatatttgct aattttaggg
     3661 gcgcgaaagg gtcaggataa acatctacaa accggacgtt tagtggaatt tacatcaaaa
     3721 gatttaacta attgggaatt caaaggtgat ttttgggctc cgggattgta taccatgttt
     3781 gaaatgccag aacttttcaa gattggtgac tggtggtatc ttgtctattc agaatactca
     3841 gatggaaata aaatactcta tcgaatgagt caaagtttgg aaggtccttg gattaagcct
     3901 aaagatgaag cttttgatgg acgggcttat tatgcaggta gaacagctta tgatggaaaa
     3961 cgtcgagtcc tatttggatg ggtaccaaca aaagatcaag aaaatgataa aaacaattat
     4021 ttatggggag gtactttggt tcctcatgaa gtctatcaaa aagaggattt ttcacttggg
     4081 gtaaaaccca tagacgaagt gtggaatgca ttcataaatt tacatgatat tgccgatgta
     4141 gagatagaaa cagcagacac aagagaagaa gtaacgcttg tcaaaaatag tggaactttg
     4201 tttaatttag aaacaacaat ttcatttgat gagaatctta gtgacttttc actgagatta
     4261 tataaaaacc ttgagacaga tgaatcttac gaatatcgct tcaatttaga tgaggggcgt
     4321 cttacatttg ataaaaaccc ttgttaccct tggtttcaat atatggataa aggacttatt
     4381 agacctatat ctttaaagcc taaccaatct tataaactaa aagtgattgt tgatgacaat
     4441 ataatgacac tttatattga tggaatcgct cttaacgctc gtgtacatgg ttgttttggt
     4501 caagcaatta ctattacagc aacaaatgga tcaatgactg caataaatac caagttttca
     4561 aatgaaataa agaaataata aacgtcaatc atgtggcagg tctgaaaatc aaacatgacc
     4621 tgtcacattt aaaagggagg ataaatgaat ggactacaaa aaaattgccg aagatgtatt
     4681 ggaaaacgtt ggaggaaaag agaatatctc aagcgctaca cactgcatta ctcgtttacg
     4741 tttaattctt aaagacaaaa gcaaagcgaa tatggaaatg cttgaggaaa tagaaggtgc
     4801 aaaaggtgtt cttttcaata gtggacagtt acaagtaatt ttcggaacag gtataattga
     4861 aaaggtttac aaagagttcg ttgaattgac aggagctaag gaagtatccg tttctgaaat
     4921 gaaagacgaa ggagtcaata attcaggaaa tagacttcaa cgagctttta aagttttctc
     4981 ggatatcttt attccgatca ttccagcttt tattggggca gcaatggttt taggaattcg
     5041 ttcgttgatt accactcctg gaatgtttgg aatggatggc tcattcgctg atcacaatgt
     5101 attcctaaat gatttatcca aatttctggg tattattgca accacatttg attatttacc
     5161 agttttagtt atgtattctg caacaaaacg ttttggcgga aaccctattt taggattact
     5221 tgtaggattt gttatgattc atccaggact agccaatcgg aatgatttcg tcatgggaac
     5281 tataaagcct gattattggc atttatttgg attaagcatt ccagcagttg cgtttcaagg
     5341 gggtgttttt ccagcaattt tgacttcttg gtttttagca aaagttgaga aaattacaaa
     5401 taaatattct cctcaagtac ttgcttacat tttagtccca actgtgacaa ttgttcttgc
     5461 caactttgca ctatttttag tgtttggtcc attggggaat tttatcggta atgttttagg
     5521 tggaatcatt gatttcctat atatgaagat gggtgccttt ggtgcttttg tttttgcagg
     5581 cgcgttacaa ccattagttg taacaggaac acaacatgct attcaaggta ttgaggcaaa
     5641 cttagtcgca acaactggat tcaattatat ccaacctatt tggtcagttt caattattgc
     5701 ccaaggtggc ggttgtattg gtatgtattt actctttaag aaaaaatcta aagatagaga
     5761 tattgcgatt tcaagcttta ttccaacact ggtaggtatt actgaaccgg ctatctttgc
     5821 tgttaatttg aaatattcaa tcgttccttt tgtttgctcg tttattggtg ctggttttgg
     5881 tggagcattt atgaaaattt ttgatgtaaa agccattgga caaggattga ctgtattgcc
     5941 aggtctgact attgctaatc cagttcttcc ttatatattt ggtaatctca tagcattcat
     6001 tttaccaatc atttttattc tcgcttatga taaatataaa gtgtttatcc ctgaagctaa
     6061 acaccgtaaa tttgcagaaa aaactgactc aaaaaaattg ccagttcaag taaagtgaga
     6121 tgagagcatg ttaaattttt ttagaaaaaa tgaaataagc gttgtagcac ctgcaaccgg
     6181 tgtttttgaa aaactggaga aattaagtga tccagtcttt tcaaaaggaa tgatgggaca
     6241 aggatttgca attgatgcaa aaaacgaaac gattgtatct ccaatcgatg gttttgtttc
     6301 aagtattttt cctacaaaac atgccttagg aataaaaaca aaatcaggaa ttgagatact
     6361 tgttcatgta ggaatagata cagttgagct aaatggtgag ggatttgata ttaaagttca
     6421 agaaggacaa aaagttaagg ctggggatat tcttatgacc attaattttg tagtttttga
     6481 aaagaatcac aagactaaag atgtcattgt tgtttttcca gactaccaag acgaaatttt
     6541 cattccgaaa cttgaagaag taaaacaagg acaagaattg tttgttcttg gctaatgata
     6601 agtaattaat aatagaatcg aggaaaatgt atggtttttg gggctattga agctggtgga
     6661 acaaaatttg tttgtgccat tggagataaa gatttgaaca tatttgaaaa agtcagcttt
     6721 cccacgacta cacccaaaga aacaatgaat ttagttttag atttttttaa tcatcataaa
     6781 gatgatttag aaggtatcgg agtgggatcg ttcggtccta ttgatattca ttgtgaatct
     6841 aaaacgtatg gcttcattac atcaacgcca aaacttgctt ggcaggattt tgattttatt
     6901 ggaagtttaa aaaagcaact taatattcca actgcttgga cgactgatgt aaatgcctct
     6961 tgctatggtg aatatgtttc tggaaatggc agaggccttt caagtgttgt ttactatacc
     7021 gtaggaacgg gcataggagg tggcgctatc caaaacggaa acttcattga aggttttagt
     7081 catccagaaa tgggacatat gcttgtaaaa cgacacaata aagacaactt ttctgggaca
     7141 tgtccattcc acggagactg tttagagggc ttagctgctg gtccggcaat cgaaagaaga
     7201 tcagggaaaa agggacaaga agtgactgaa aatgacgtta cttgggaaat agaagcttat
     7261 tacattgctc agtgtgcata taatacaaca ttgatgttat cacctgatgt cattatattt
     7321 ggtggcggag taatgaaaca aaaacactta aaaaagaaag tacaagataa gtttattgag
     7381 ttgatcaacg gatatgtcgc tattcccgat gttgacaatt atattatgac accaaaattg
     7441 ttagataatt ctggtattgt aggttgttta gctttagcaa gagacgttaa agaaaaatct
     7501 taatttggaa aaaagatcgt ctcgactatt tgtttggttg atgcgatctt tttttcaatt
     7561 agtaattgaa gggacgccta aaagtgaaaa aaaaaatgaa agatgaagta ttagagtata
     7621 tcaaaaataa tttaaagtat tacgattttt ctgctcaaga tattgcaatg aaattttgta
     7681 ttaaaagaaa tgtcgcttcc cactatttga atcagttatt ttcagatgga aaattactaa
     7741 agaatgattc tgttagacca gtaatgttca agtataatca acaaaagcca aaagactgct
     7801 tttctaagtt tattggagca gacatatctt tgaaatccac tatagataaa tgcaaagcta
     7861 ctgtaatgta tccccctaat ggcctcccat taataataaa aggaaacagt ggcgttggaa
     7921 aaagtttttt agcaagtttg atctatcagt atgctcttga tagaaaagta attcataatg
     7981 acgcaaaatt tgttgttgtg aattgtgcag attatgcaaa taatccagag ctattgtcag
     8041 cagttttatt tggatataaa aaaggagcct ttactggggc tgaaaaagat actgcaggga
     8101 tgctatcttc tgctaatgga ggataccttt ttttagatga ggttcacaat ctaagtgcag
     8161 aaaaccaaga gaaattgttt cttcttatgg attctcaaaa gtatcgtaaa cttggtgaaa
     8221 gtgtgcaatg ggagtatgca aatgttcgcc ttatcttagc cacaacagaa gataaaaata
     8281 cctctttgtt agcaactttt agaagacgca tcccagctga aatcactctg ccagattaca
     8341 ataatagaag tacatctgaa aaaattcaat tattatttga atttcttaaa gatgaagcaa
     8401 ttacgattga ttcaaaaata ttttgttcga ttgaactttt tactgatctt ttatcgaaaa
     8461 catttgaagg aaatgttgga gaattgagga atgaaatcaa gcttctttgt gcagaaggtt
     8521 atttaggaaa taagagaaag tcttcaattt atttagggga caaactagac agcggttttt
     8581 ggatagatcc agaaattagt atagatttaa aaaaaatgtt tcttaaatca ctttctgaaa
     8641 ttgatttgat tgaacttttt caaaatcaca tttcaattga agaaagcatg agtcaattaa
     8701 gagatagaat acttaaagag tgtgcaggtt cttctgagta taattattta gaaagtgacg
     8761 aatttatagc attaagaaac tatgttgtta ataaaatatc accaattatt gattcgacag
     8821 gtctatgttt attagatgaa ttcttggcgg atatcagtct atttataatg tttattgatg
     8881 taattgaaga aacatcaaga aattttttgc tgtctaagtt cagaaaattt cgtatgaaaa
     8941 atgataaaca aaaattattg gcaaatgaga tttggagtag tttagaagtc gaagaaagta
     9001 aacaagaact tttgttgaaa tggatacttt ttcttgtaaa gaagttctat gtgaaaatac
     9061 cagaaaccca ttgtctgata gttatgcacg gtaaaataac agcttctgcg atcgcaaggg
     9121 aaacaaataa gttgctgaat acatatgttt atgaggcttt cgatatgcca attgaaggtg
     9181 agactagtga cttaattaac ctaatcaaca atttttgtaa aagtatagat acgacgaatg
     9241 gactcattct gctagttgat atgggatcat tggaacaaat gtatgaaaaa attgaatcca
     9301 atgtaatagg tgatttggta attttaaaca atgtttctac agctttagcg attgaatgtg
     9361 ggattcaagt ttgccaaaaa aagccaatta gtcattttta ccagatggat tttgattcgt
     9421 ttcaagttaa agttcagtat tataaaggat tatcacaaaa gaaaaatgta atagtatctt
     9481 gtctctctgg tgaggggatt tctgaaaaag ttaaggatat tttaaaacgg tatctcgata
     9541 atcaagtgga gattttgatt tttgatttta acgcattaa
//
LOCUS       DAZUKZ010000101         9340 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000101 DAZUKZ010000000
VERSION     DAZUKZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.103"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..376)
                     /locus_tag="ACG8EF_002259"
     CDS             complement(<1..376)
                     /locus_tag="ACG8EF_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS4947546.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERK"
     gene            complement(366..2540)
                     /locus_tag="ACG8EF_002260"
     CDS             complement(366..2540)
                     /locus_tag="ACG8EF_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HFS4947547.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2556..4898)
                     /locus_tag="ACG8EF_002261"
     CDS             complement(2556..4898)
                     /locus_tag="ACG8EF_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS4947548.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4920..7463)
                     /locus_tag="ACG8EF_002262"
     CDS             complement(4920..7463)
                     /locus_tag="ACG8EF_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4947549.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7473..7871)
                     /locus_tag="ACG8EF_002263"
     CDS             complement(7473..7871)
                     /locus_tag="ACG8EF_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFS4947550.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7952..8236)
                     /locus_tag="ACG8EF_002264"
     CDS             complement(7952..8236)
                     /locus_tag="ACG8EF_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947551.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8260..9258)
                     /locus_tag="ACG8EF_002265"
     CDS             complement(8260..9258)
                     /locus_tag="ACG8EF_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4947552.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2653 a         2002 c         1461 g         3224 t
ORIGIN      
        1 tttttctctc aatttttttg acgtttctat catgaatcac gagaaaactt gggacacttt
       61 taacatggta acgttgtaaa aaagtttgaa gttcttcttg tcttggttct tttcttgttt
      121 caattcgata tacattccta gagaattttt tgtcgtatct ttccaagaaa gattcaactt
      181 cttggcattt tttacaatca tttcttgcta gatacacaaa aaatgattga ttcgtttttt
      241 gaagttctag tacttcttct aaattactat tgattagttt tccatttgat tcttcgttag
      301 tttttatcag tttaaaacaa ccaaataaga ggaaactaac aataagtatc ctaacaataa
      361 ctttcttatt tcgcatattg tttctccaaa tttggtttcc gaatatagac gactcgcggt
      421 ggttcctgga cgtcttcagc tgttcgatac accacaaatt cgcctgttct caatgctcga
      481 atgcgattcg gattgaattt aaagcggtct acgtctcgtt tcgtgcctct ttctcctgtc
      541 caatcaacct ttcccatatc agaataacct gcttcttgtg tcgtcatttc cgttaattca
      601 atgtcttctt ctgtccccat taaatctgcc caataatcaa tttctgtcgg gtcatttgtc
      661 ttcccaataa agaacgtatt cacattccca atcaactgtt ttcgaaggac aggatcaatt
      721 ctatcaatgt ccgcaagcgt ttgaggggca tacgccgcat gcaccccagc gcctcgtccc
      781 ttattgacta agtctataag gttatcattt agataagaag ctggttcgtc aaatattact
      841 aggactttcg agcgattggc tgtttctcct gcttgtttat acacttctga cgcaaagaaa
      901 ttaatatctc ccacaagcat ttgagccaat gtccgaataa actcactata aatcaatccg
      961 tttaacgaga tataaacaat ctttccttct ttaacaactt cttgaatatc taattctctt
     1021 tttcctccag aagtatcaaa gagttcacca agctcactat ccaataacat attgatattg
     1081 gtctgtaaat tctcagaagt cgcatacaaa taaaacgggt tcttcttatg ttcgtaacga
     1141 acaaacaacc gtttaaacaa cgtttgctcg atttctgtca tgagagaaat gttttctttt
     1201 aataatagat aatacgtttc taagtcaacg gttttacgat catgttcaac gattcgctct
     1261 tcacttaact gttccacatc ggcaaaatca cgttctgttt tgtccatcgc ttcattcttt
     1321 ttcgtttttt tcttcgtggt tttcttcttt tctacgacca ctttttcttt aaataaatct
     1381 ggatttttca actgaatacg atccgcaaac aaatgtaaga cgtttcgtgg caataaataa
     1441 tgttgtaacc aaactagatt tctttcaatt ttatggctct tttggaactc gtctaaaagc
     1501 tgaatcgtta cctgtaataa cgctttggct gctgtcgaat aatacacgct ttctgtttct
     1561 gctagattgg ttaatttatc tcgaatggca atatcattgc cattttttac aggattataa
     1621 cgcaaatcgc caccgtccac aaagacttca atcgggcgat tgtatgcctc tgccacttct
     1681 tttaacgctt gtaaagtctc tcctgctcct ttcccatcga ctaagataac ggggatacga
     1741 aacttacacg catgttctgc tagtaattgg atcaccgtcg tttttcctga acctgtagtc
     1801 gctggaataa tcacatgttg gttcaattca ctaaagttca tcacataagg gagcccatac
     1861 tcatctgttc ctaatagaat cactttggct aatgaagcca gttgtttatt ccgcaaagca
     1921 acgtctttta tgacaattgc tacctggtac gcttgctgat actctgctgt atatttttgg
     1981 gactcctgaa atttattctt ccgacgttcc ttgtattctt tactatttag caaagctgtt
     2041 tgccgttctt tctttgaaac aacttttctt ttttcaataa aagacgcata cgcaatcaaa
     2101 caacttcctg cacctgtccc acctaaaagg agggcaaaaa tcgtttgtcc attaaactgc
     2161 aacgcatgag aaaacaagtt taatggaaat aataagtaac acgacaaata ccctctagga
     2221 agaacaaaaa taaaacaaaa tagatttaat agcgtaaaac ctaacgcaaa aataaaaaaa
     2281 agagagcttc ttcgttttaa gaagctctca tactcaaagt cataaatatg tgcacgtgtc
     2341 tcttttttat ctatcataaa gacaatcgct aaaggaatac tgcctacaat aaaaaatgga
     2401 tatagacaaa cacctaataa agtgacgata aaataaagtt gttgaaaccg actttttggc
     2461 gtttcttttt cttgttttct tttgcgatcg acagaaaaca tttgctcaaa atgtgtcggt
     2521 cgttttggtg cattcatcat actatcgatt tcctcctatc gttttcgttg tttcatttct
     2581 cttcgataaa tcggttgttt ttctcgtttt cgtttcattt ttgcttgagt tgctcgaaac
     2641 tcactccgtg tcttcggtgc ttgttttggt gattttgttt gtttcatggt tgagtatgct
     2701 ttagatacgg ttgaaggcgc tttggtttgt gctgttactc gattgactgc tgacatatgt
     2761 tcccgaaccc cctctggacg tcgaacagat ttcggtgtgg tagaacctgt tgctcgtggt
     2821 acattccctt ttcttagtgg tttttgtacc gcatgttttt gtcctgcaac tgtccgggtt
     2881 ttcatcgaac ttgtttttgt tacgggagta agtgcctcct tccgacgacg acttgtttgt
     2941 ccctgacgac gttttcgatc gttcaattct gttctaggcg tatgcttctg gccataagac
     3001 ttgtgttttt gctttggatt cgctaatgac accaccattg cagaaccctg attgcgatac
     3061 ggactacgag acgcaccttt tctacttctt tctcttagac taggattgcc ttgcaattct
     3121 cgttgtaata aatcggctgt ttttcttgcc tgtttctcca ttgctgttcg ttgcatacgt
     3181 tcttgacttt gctcttctgc ttgtttcgct tgatcatgca agtctcttgc tttttgtgcc
     3241 atacgttcca tacgatttgg tgctttatga cgaggttgtt gtgcggattc acgccgaaaa
     3301 gcacttcgtg tggcttgttt cgccgtttgt ggcatacgaa cgtctgagcc acccccacgt
     3361 tttcgttgac gttcttgtct cttccgtgcg ttttcttttt ctttttgttt ctgtgctttt
     3421 ctcgcttctt tgcgttcttt caaccgttgt ttggcttccc ttctcaattt tgcttctgcc
     3481 ccaaatggat ttcccgcaaa agctaacgct cctcgaacac ccattcttcc agtaaataga
     3541 ctgcctaaaa agcctcggaa caaataaatt cccaacggcg caaacaaata aacaatgatc
     3601 aagagaattt tttcccatgg atcgtcttgt gtactactta attggaaccc gtaactaaga
     3661 aaagaagaaa aaaggataca tgcaaaacct gtcattcctt ttaaaaacgt caaaatacca
     3721 aaaactttgg catagttacc aaacacattc attgtgacaa gaaacaatcc taaaaaaagt
     3781 aagaacggca acatcggtac taacaatagt tgcatagttc caataatcaa tcgaaacata
     3841 cttaagacaa aataaacaac ggtttgaatc agattcacga caaaataaaa caaggcaaat
     3901 gctgtatttt tccaactatt gtaatattgt atgttcttgt ttttcagtgt atctgcttca
     3961 tactctgtga ctttgtcatg aattttctcc cctatttttg tacctaaatc attgtccaat
     4021 aaaagattga ttcgatcata ttcttttcca gcatatttga ctttcttctc acgaattgtg
     4081 tctacattgg ttgtgccata atttaacaac agatatggtt cataaacatt actataaaag
     4141 acccgagccg ccatcaattt gcctgccgtt tccacttttt tatccgctgt tacctcttct
     4201 gtttcttttg tttcataatt tgtttcttct tcaggtgtta acactggatt gaccttcacg
     4261 aacgccgttt ccactgtaga atctaaagaa aaaagaacat caaaaagatt cttatttgtc
     4321 ccagaatctt ttaatacgct taacccagta aaaataagga tcgtcataag aaagacacgg
     4381 agtgcttgtt tcattcgagc ctctccagca tatttgattg ccatcataat tgccgaaaaa
     4441 ctgattccaa tagttcctgc aatacttaac atattgtccg caattttttg tgttaagttt
     4501 tgaatgggtt ctcgaatggc ggaaataata tctaattcaa agagaaattc aaccattgaa
     4561 gcattgaatt ctcctaacat ttttacgcca ctccaaacaa aatttttcat cactcctgca
     4621 acattgtacg atagcttttt tttaaggttc attgactcat agtcattggt catcaattca
     4681 aacgaattct ttttgtaatt tttatacaat tgatccacat cagtcgttac ttcttttact
     4741 tctactttat cgttggcatt ctctccttca gaacgatcgg tttcagtgac acccgcacca
     4801 tcattcattt gatcggcaga aacagtagcc ccttgtagac agaaaaaaag accccctaat
     4861 aaaaggagcg tccaccattt cagagatttt tttcgcaaaa aaaccacctc ttttttgttt
     4921 tatcgttgtt tttcattttc taacattgct tgaatttttt catcttgatt ttcagtagaa
     4981 gaaacagcca ataaccaact atcaaacaac acatcaatgg caatcggttg atttcttccc
     5041 aaatgatcct ggaataaaca ggttcctgtt tgtaattgtt ccatcatttc taaattggct
     5101 gaattcactt ccatatcaaa gaaggacaat agtttctctc gtgcttcttt ttgtttcgga
     5161 cgaaacgcaa atttgtaaga aattaactct tttttatctt cttgatcgta atctttaaac
     5221 gcttgcacaa caaggtaaat atccgtatta ttcgcacgtc cttttcgcaa gctatcatca
     5281 atcaaatagc ttccttgtgc ggtatcttca tacccttttg cctcgtcata aataatcgca
     5341 gcgtcttctt ctttgtttgt agaaaagata tacgtaaact tcataatgac ttccataatt
     5401 gccattcctg caagttgttc gctatttaat cgacccgaat caatctcttt ttgtgtcgga
     5461 atgcgtattc cttgtgtccc taacacgttg atttgtgaat caaaacgtat cggcgtactg
     5521 tgctcattcc caatgagaat ctttcctaaa ccaacgctat gccctaaaat caatttagct
     5581 agttctttgt cactttttcg aatttcttcg ataacttgac ttaacgaatg cttacctggc
     5641 atggtgtaaa ctacatcgat ggcgtcttgg atcaccgttt tcttgtggct cgctgtttct
     5701 cgattggtat ctacttctcc taacattcct aacacatttt tagcggtttg aattgcctct
     5761 tcttttggta aaaacaatag aggatcaagc atccctcgac agctttcatc tgaagataac
     5821 gaaataaaat taattcgttc atacatagcc acaaactctg gtatgtgtcc gaatttttca
     5881 agtgccttta aaaagaactt ttgggtttca tttttcggat caatataaag aattttttga
     5941 cctaaaaaca tcagccataa aaaaatatat tttacaagga ccgattttcc tttacctggt
     6001 ggaccaataa tcaacgtatt tccattggta tgttccgcgc cttcaatact tttctttgtt
     6061 aaattgggat aataccatac tagtttacta gatagcgcta acgcttcttt cgcagattta
     6121 aacttttttg caataacaat ccgtcctaaa ggaaatcctt tcttatttcc tacttctttc
     6181 tttaagtcca agccataatc tgccacaaaa cctgtcgtag cgacctgctc gtaactacga
     6241 aaacgttgcg tacttccaat taagcattga ttaaacaacg ttagctgatc cgctaccgat
     6301 cgataaaatt tgaaatctgt agactcaatc gcaaattcaa tatctttgac tcgttcttcc
     6361 aattcttctt ttgaagaagc agatacaacc agcgtgacgg tagaataaca caatcgtttt
     6421 tctttgcgct tcagcttatc ttttacatcc attaaccgtt ctgtaccaaa atttaataca
     6481 tcgtctgaat ctaaaatgcc gtcaacggtc tcatattctt tttcttgttc ataaatccgt
     6541 ccagccattt tattaatttt tcgttcatca gattttgaat gctcaaatcg aagtcgaata
     6601 tgcgtatcaa ttggaaaaga taagctatcc tgaagatttt gaataaagct actcccatac
     6661 atggctaaag gagcgtccgt ttgacttaaa aacgtcgaat aatgcgtttg attcaactgt
     6721 tcaatcgcaa tgtatcctgc ttcattcgaa atgacgccct cgcaaatttc cattggcgta
     6781 agctgtcttt caggcaattt ttcttctgat cgatggaaaa aataataata aatttgtcca
     6841 atctcttggg cagataagcg ctctatcggc ttataattgg acaattccgt atagagctcc
     6901 ttttctaatg tttcatacgt tggtaacaca ctacttatag gaactcgttg tcccgtaata
     6961 cgatagataa actttttaaa aacatgctta agcaattgaa aatattcgat cggatcagac
     7021 actttcattt ctgtagtgaa acgcacaaac agatacgttc gatactcgtt taattgcact
     7081 tcgtctttta aaatctttcc tgcctgttca aagtacactt tcccaagatc tgcgcattct
     7141 ccctttacca aatctcgaat ggtttcttca atgtgcgtat ctaggttaaa ttgacgtgga
     7201 atattgataa aatgatagtc atattcatca tgtccaagta tgccttcgcc atcttgaaga
     7261 tagttcttga aaaaatcaat gtcatttaaa ggaaatactt gggaagaaag ttgatagcct
     7321 acccacacat catcattttt ggtcaaaaca aaatttcgga taatggcgtc tatcggaaat
     7381 tctaatcgag gaagcttcgt ttttttcttt ttgaactgcc aaatcccgat aataaagacg
     7441 accaaaagaa acaaaaacgc catttactcg actcactcct tccatttgtg tagggaatac
     7501 gtcacttttt catggaaaaa cggcacatac atttcatgtt caaagcgttg tttccgtttc
     7561 tgataatagg acgttcgccc aattaaatac tcgaaaaagt gtttattgtc ccatttcatt
     7621 gaaaacaatg tccaaacgag aacaccaatc ccaaaaatca taatcaacca ggactttcga
     7681 aacagtgtct gcaagaaact aatttgattc acatacgcaa tcaaaaatag gatcaaaaga
     7741 atgaagagaa ataccccacc tatgactaat tgttccatgc gaatgccatt agaaatccag
     7801 ggaatttttt gtcctttaat cccgtaaatt ttgtgagggt gcttaaactc ctttgtatag
     7861 ttgtattcca tgttcactct acctcctttt acaaaaaaag aagcgaccag aagttctaat
     7921 cttctgatcg cttttgcagt ttgtcttttt cttatgataa ttgtatccag ggcacaatca
     7981 tttgcgttac ccccacgttc tcttcactat ccttaataaa gataatgttc tttagccatt
     8041 caccaatatc aacggcattc gataacaata agacgagcat tatcaaaata aagacgccaa
     8101 taatggcaaa cgctgcccgc gcaaagcctt gggtcgcata agcaccaacc attagaacaa
     8161 tcgcaacgat tgccaatagc caaatgactt gtccagatac atcatcaaat aacgatttaa
     8221 gatcaaaaag tttcatttcc attctcctct aagttcattt tattcattta acgtagtaac
     8281 aaaataacta tcgccacttg tttttacttc cattgaaaag tttgattgga actcgtttcc
     8341 tttctcaaaa tagaaacgat acttcccttg caaaaagtag tttccttctt tattttttac
     8401 tgcatgatcc agttgtacat tgatcaattc gccgccttct aacccttttt cgttagaaat
     8461 caatgccaat ttttcatcat ttttgacata taactcaaaa aaccgtttca caaatgcttc
     8521 cagcttgatt tcttcgcttt cagtgaccgc ttgcccagaa gaataaaatc gattttcctg
     8581 atagttcaca gaattggtat tcttactctc agaaagattc aaaaattgaa cgccatcaag
     8641 tacttttaaa acattgtttt gataactgac tggaaaagaa agttgactgc ttttccaatg
     8701 ttctccttct gtaaaagtaa cctcataaaa taggcgatac attgcttgtg tcaaatcgat
     8761 ttcttctagt ttaataaagc gaacatcttt cgctactcga ttggtagccg tcgttacaaa
     8821 gcctagatca gacgcttcca atccttgtgc taaataatga ttgacggaag cctcccattc
     8881 gtttcgtttt tctggcgaat aagtaaataa cctttcaata aaatgttgtc cctcgtactt
     8941 caattgttcc gtagaagaca aggtttggtt gacttcagtt aacaatgttt ctggattgac
     9001 ttgttttttc actgctaaac gcgttaatgt atccactcgt ccaaagttta ggaggagcac
     9061 gacacccatt aacagaaaca aacaaaaaaa agcgcctgaa aatacactca tggcactttt
     9121 ttgcgaaaat tggatcattc gatctgacgg ttctgttttc tttttttgga acaaaaaacg
     9181 tttcttttgt ttcttttcct tctgttgttt cggtggctta gtttggaacg cttgtggatt
     9241 aaattttgca ttactcatga gaaatgccat accgttctaa caataatttc ggtattttgt
     9301 ccacaatcac tacttcgtgg acaaccacac ttgccccctc
//
LOCUS       DAZUKZ010000102         9281 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000102 DAZUKZ010000000
VERSION     DAZUKZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.104"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..680)
                     /locus_tag="ACG8EF_002266"
     CDS             complement(12..680)
                     /locus_tag="ACG8EF_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4947553.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            814..1101
                     /locus_tag="ACG8EF_002267"
     CDS             814..1101
                     /locus_tag="ACG8EF_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947554.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1517..3055
                     /locus_tag="ACG8EF_002268"
     CDS             1517..3055
                     /locus_tag="ACG8EF_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFS4947555.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3052..4017
                     /locus_tag="ACG8EF_002269"
     CDS             3052..4017
                     /locus_tag="ACG8EF_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4947556.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4004..5326
                     /locus_tag="ACG8EF_002270"
     CDS             4004..5326
                     /locus_tag="ACG8EF_002270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4947557.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5389..5493
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5567..6310
                     /locus_tag="ACG8EF_002271"
     CDS             5567..6310
                     /locus_tag="ACG8EF_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4947558.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6325..7317
                     /locus_tag="ACG8EF_002272"
     CDS             6325..7317
                     /locus_tag="ACG8EF_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4947559.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7329..8066
                     /locus_tag="ACG8EF_002273"
     CDS             7329..8066
                     /locus_tag="ACG8EF_002273"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4947560.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMSQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8056..8778
                     /locus_tag="ACG8EF_002274"
     CDS             8056..8778
                     /locus_tag="ACG8EF_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS4947561.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8862..>9281)
                     /locus_tag="ACG8EF_002275"
     CDS             complement(8862..>9281)
                     /locus_tag="ACG8EF_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS4947562.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3037 a         1602 c         1963 g         2679 t
ORIGIN      
        1 aaaaataaga atcagtgctc taactgttca agaggatcaa gtggttcaaa tgtttctgta
       61 gtctgttttg cttccaattg gggcaaaaca gatccaattt ccagatactt cactttattc
      121 aatgtataag atagaataga acagctaagt aatgtataca gaaaatgtgt gagatccaaa
      181 taaagcaagg agacaactaa aataccaaaa gtaatcaaga aaagactttt gccaatctta
      241 atatgaaaat atttttcaat ggcttgataa atgatatctg ttccgcctgt gcttccgccg
      301 aatcgaaaga cgagaccgca accaatgcca gaaagaatac ctgtcatcaa actattgatc
      361 agtagactat ctacagcaaa atgtatatga aggctctccc ataaatgtaa aaagaaaact
      421 agcgcaaatg ttccgacgat cgtccggaga aaagcgtgtt ttccaaaaag aaacaatgag
      481 ataagcaata aaggtaaatt aaaaagcagt gaagtgacag aaggagcgaa tccaaaaaga
      541 ttatgcaaaa gcaaagcaat accagtaacc ccaccatctg ccagcttaga tggaatattg
      601 attcctgtca cagctaatcc gtagattcct gcgcctaaaa tgattccgca caattctgta
      661 aataaattta attttttcat ttctagcctc ttttcttttg aaaattttca gcaacttctt
      721 tattatactg acttatactc ttacttcaag aggaaaatga aaaaaaatta tttttggctg
      781 tttcttttta tttatcaaat aaggagtgga aaaatgcgag cagcagactt attgacaatt
      841 cttacaaaat cacccaaaaa aacagagtat caaggagttt ttttggctca agaacaaaaa
      901 ctggtgcctg tgacaatggt ccagataaat aaagcaaatc aatttatctt tttatttgaa
      961 cacggcaaag caccacttgc tatgaaagac atctttcttt ttttgatgaa gaaccgtgag
     1021 aaagctgttt attatcagtc aaaagaagaa ctcattcctc tttatggagt gaaagagata
     1081 gaaaataaag tggtgatata agtaaaaata agaaaacaag acgtttatga tgtcaaaaca
     1141 accatttttt acatttttgg caaaaaagtg tcatgataca gtataatatt cgttgaacag
     1201 gccaataaag gtgttctccc ccacgagatt tctactttat tggctgtttc tttttattaa
     1261 aataaaagtt gtgtgtgtaa aaaagatata gctgattcca agtgacaatt tcgtatagat
     1321 ttttatggta ttcgtagtga aaaaacgaga accaaaaagt tgatccgact ttgttgcttg
     1381 gattcttttt ttgtcagcat atttagaggt atttttttct tttttttgtt cgtgctgtaa
     1441 ggttaaggca agacaagtta gttgggaaaa aggaaagaaa tgtttatact taaagaataa
     1501 cagaaaggaa ggtcgcatga ataattttat acaaacatta agtgaaagaa aaggcgagtt
     1561 gctggaagct gcatttcaac atctgtccat ttctctcatt tctttgctga ttgcagcact
     1621 gattgctatt cctttagcga tttgggcagc caatcataaa aaaatggcag aagtcttatt
     1681 gcagatcacg ggtgtgctcc aaacgattcc ttcacttgcc ttattaggtt tacttatccc
     1741 atttgtaggg atcggaactg ttccagcatt gattgcactt gttatttacg ctttgcttcc
     1801 gattttccaa aacacgtata ttggtctagc tgaaattgac ccgtctattg aagaagcagc
     1861 ggtagcattt ggtatgtcca gaatgagacg cttgctgaaa gtagagcttc ccatcgcatt
     1921 acctgtcatt atctcaggta tccgaactgc cttagtttta atcatcggta cggctacttt
     1981 ggcagcattg atcggagcag gtggattagg gacatttatt ttgttaggta ttgaccgaaa
     2041 tacacctgtt ttgacattga ttggtgcgat cagttctgcc ttgcttgcaa ttatattcag
     2101 cggactgatt cgctggttgc agcataagaa acctcgatat tccatcatta ctttagcagc
     2161 agtggtgtgt ttatttggcg gcgtttcttt atatcagtct gccttgttta aaaatgaaac
     2221 catcacgatt gctggaaaat taggagcgga acctgaaatc ctgatccaaa tgtataaaga
     2281 attgattgaa gacaacacaa aaacagaagt tgaattaaaa ccgaattttg gcaagacgag
     2341 ttttttgttc aatgcactgg atcaaggtca aatcgatatc tatcctgagt tcacaggtac
     2401 tgtcctagaa agcttggtga aggtacctga agcaatgaaa agtgctgatt tgacaaaaga
     2461 aaaaacatat gacgaagcag aagaattatt gaataagcaa ttcgatatga cattattaaa
     2521 accaatggct taccaaaata cgtatgcctt ggcagtcaag agatcttttg cgaacgaaca
     2581 tcaacttaaa aagatttccg atttggcagc tatctctgat cagatcaaag cgggattcac
     2641 actggaattt attgatcgtt cagatggata taaaggaatc caagaagcat atggcttgaa
     2701 tttttcaagt gtacaaagca tggagccaag tttgcgttac caagcaatca ataatggaga
     2761 tgtcaatatt gtggatgcct attccacaga tagccagctc aaggagtacg atctagtgac
     2821 tctagaagat gatcgagggt tattcccagc ttatcaaggc gcaccgctga tgaaaaaaga
     2881 ttttgctaaa gaacatcctg aaatcgttaa agcactgaat aaattaagcg gaaaaataac
     2941 ggaggatcaa atgattgata tgaattacca agtcaatgtc gaaaagaaaa agccgacaga
     3001 agtagcacga atcttcctga aaaatgaagg attagtaaag gaggaagatc agtgactaaa
     3061 ttaatcgagt tcaaacatgt gcaaaaaaag tatgatggaa agtatgtcat cgatgaccta
     3121 aatctggcaa tcaaaaaagg agaaatcttt gttttggttg gtccttccgg tagtggaaaa
     3181 acaactactt tgaaaatgat caatggatta tctaaaccat cagcaggaga catctacttc
     3241 aaaggaaaat cgttaaatga atataatctg cagaaaatgc ggtggaatat gggatatgtg
     3301 ctgcagcaaa tcgctttgtt cccgaccatg acagtcaagc aaaatattga agtcatccct
     3361 gaaatgttag gttgggaaaa gcagaaaaga gcagaccgtg tagatgaact tcttcaaaaa
     3421 gttggcttat ctcctgatat ttatcgagat cgtatgccta gagagttgtc tggaggagaa
     3481 cagcaacgga tcgggatcat ccgagctatt gctgcaagcc cagatgtgat tttgatggac
     3541 gagccattta gcgcattaga tccgatttca agaaactcat tacaagaatt ggttttgtcc
     3601 ttacacgaag aattgggcac gaccatcgtt ttcgttacgc acaatatgga agaagcaatc
     3661 aagctaggtg atcgtattgc atttatgaag gatggagaaa ttatccaatg tgacacacca
     3721 gagcagttat tgatgaatcc taaaaacgac tacgttcggc atttctttga tgaaccgaaa
     3781 cagacgaaag agtggcgagt agaagattta gtcgtcaatg gatatttctt aaatgaaata
     3841 ccagaaaatg ctcgtcaaca agtttgcttt gatacgccta tgaaagaagt atattcgctt
     3901 ctttcggtgt atccaagcat tgtgatagta gaaaagcaaa gaagtattgg gtcgctgaca
     3961 agtaaagaaa tctttgcttt tttaagtcga gaggaggaag gaaatgaaaa catatgatgc
     4021 aatcatcatc ggttcaggtg tgagcggctt aagtgctgct tatggattga aagaagcagg
     4081 taaaacagta ctagtggtcg aagaagatct atggggcggg acttgtccta accgtggatg
     4141 tgatcctaaa aaagtcttgt taagtgcagt agaggcacgt aatcgggtaa aacaattgag
     4201 tggaaaaggt tttaacgaga tcccaactgc caactgggaa gaacttcaaa aattcaaacg
     4261 aacatttacg gatccagtcc cagaaagccg aaaaaaacaa ttagcagaag ctgaaatcga
     4321 tcatctttca ggaaccgctc gttttttgga tgattcatct attgaagtca acgaagaagt
     4381 cttccatgcg gattacttag ttttggcaac cgggcaaaga cctacgatct taccagtgga
     4441 gggaaaggaa tacttgaaaa ctagtgcaga ctttttatca ttgcctgtac tgcctaaaga
     4501 aatcattttt atcggtggtg gttatattgc ttttgagcta gctacgattg ccaatgctgc
     4561 agggagcaaa gtaaccattg tccatcataa tcaacggcca ttaaaagaat ttgaggcgtc
     4621 tttggtagag gaagccgtcc atcaaatgga agcatctggc attcagtttg cttttggtgt
     4681 tgaaacccaa aagatcattt cagaaggtac tcgctatcgt ttagtcggaa aagagacgga
     4741 attagtagca gatatgattt tttgtgcgac tgggagacag ccgaatacag aatctttagc
     4801 acttgagcaa gcgaatatcg tctttgataa acacggaatt gcagtcaatg attatctgca
     4861 aacgagcaat ccaaaaattt ttgcgtgtgg agatattgtt tcaaggaaaa ccccaaaatt
     4921 gacaccagtc gccacttttg aaggaaatta tgtagctaag cggataacgg atgcaacaag
     4981 cgaacctatc aaatatccga ttatacctac gatcgtctac gccagtccta aacttgctga
     5041 agtaggagtt acaaaaagtc acgcctcttc gtcagatcaa gtggtagaga tggatcttac
     5101 cagttggttc acctatcatc gtgtcaatga accagttgct aaggctgaat tgacctttga
     5161 ccagcaaaat tatttgattg gtgctgcagt gattagcgag caagcagacg aattgatcga
     5221 tgatttgaca ctcgtcatca atcaaaaatt gactaaaaaa gagttggata gctatatcat
     5281 ggggtatccg acattggcaa gtgatctctc ctatttattg aaataatcag catgataact
     5341 aaagcgcttg ttgctttttc cttcttgtgc tacactttaa aaaatgaata cctgtttctg
     5401 gggatgtcgt agacgacatg agagtaagtt gtgaacttag acccattgaa cctgtcagtt
     5461 aatactggtg aagggagaaa catagcagac gcttgaagcg ggttactatg tcgtgcccat
     5521 ttcaagcgtt ttttgttatt catattagaa gaaaggaggt aacaaaatga agaaaaatct
     5581 ttatccagca gtcagtatgg cattcatttt aattatttgg cagattgttg tcatagtttg
     5641 gaaaatacct gcctttattt tacccggacc tgctgctgta ttttctgcat tagcggcaga
     5701 tgcacaagta ttactttccc attctgtcac gacattaaat gaagcagtgc ttggtttggt
     5761 aatagctagc gttctttctt ttattactgc cttggcaatg gattattgga agtttttaga
     5821 aatgtctttt tatccgttac tagtgatttc acaaatgttg ccaatcatgg tgttagggcc
     5881 attacttacc ctatggtttg gtttcggtat gcttccaaaa gtgatccttg ttgtcttgat
     5941 aagctatttt ccgatcgtag tttctttttc ggatacattg aggaaagtat caaaagagca
     6001 aatcatcttt ttgaaaacga tgggggcaga tacagggaag atttatcgaa tatataaaat
     6061 accagcgggt ttatccggct tcttttccgg tttaaaagtg gctgctactt attgtgtcag
     6121 cggagcagtc gttggagaat ggctaagcgc tcaagcaggc ttagggtatt acatgatacg
     6181 cgtaaaaaac agctaccaga tcgataaagt ttttgctgcg atcatttgtg tcattctatt
     6241 gagtttattg ctgaatggcg cttgttcatt attgaaaaaa gggtattatc gtatgcttta
     6301 tattttttaa gagaagggga tcaaatgaaa aaattatggc ttttattacc gctactgttg
     6361 cttttatccg cttgtggcac tgcaaaagaa acaagtagca aacaagaaat aaaggatttg
     6421 aaaaaagtga cacttgtttt ggattatgta ccgaatacta atcatactgg catctaccta
     6481 gcaaaagaaa aaggctatta taaagaggct ggtttaaatg tccaaatcat cgaaccagga
     6541 gataattcga caagtattgg tctagtaggt gcggacaaag cacaatttgg tgtaagttat
     6601 caagaagatg tcacttatgc tcacgcagat ggacagaata taccagtcaa agcaattgct
     6661 acagtgatca aacataatac ttctggattc gccaccctat cagacagtaa catccattca
     6721 ccaaaagatt ttgaaggaaa aacatatgca ggttggcaaa gtccaagtga agaagctgtc
     6781 ttaaaagctg tgatggaaaa agacggcggg gacttttcaa aactgacaat ggtaggaagc
     6841 aatggagaag ggccagaaag cttaggaaaa tcctcagata tccaatggta ctttgaaggt
     6901 tgggatatga tcaaagcaaa agaagcgggt attgaactaa actatatccc attgaaagaa
     6961 ttagatgaac ggttagatta ctatactccg gtaattatta cgaatgatca actgatcaaa
     7021 agtgatccag aattggtaca gtcttttatg gatgccacga aaaaaggcta tcaggaagct
     7081 attaaggatc caaatgacag tgctaaactg ttgcaaaaat atgcaaaaga aaatgatcgg
     7141 acgtttttag aagaatcaca agcattcttg tcaaaaaatt atacggatga tcctgagaat
     7201 tggggattga tggaagaaaa agtttggcgc aattatacag cttttatgca ggaaaacgga
     7261 ttgatcaagc aagatgtacc ttcgcaagaa ctattcacta atcagtttat aaaataggag
     7321 tgatagatat gtcaatccaa atcagccact tgaatgtagt cattgaaaac ctgaaaatat
     7381 tagaagacat ccatttcact atccctaaag atacctttac atcgattgtc gcgccaagcg
     7441 gagccggtaa gtcaacattg ctgaaaactt taacaggagt ccagcctatg acttctggaa
     7501 cgatcaaagt agacgatcaa aaagtaactg gtctccatac agcatttagt tatatgtcgc
     7561 aagaagatat gttacttcct tggctgagcg tctatcaaaa cgttacgtta tatcaaaaaa
     7621 tcaatcatct aacaatcaat gaagaacaag taagggatta tctggatatc tttgggttaa
     7681 acggctatga acattttttg ccggaacaat tatctggcgg gatgaagcag cgaacggcct
     7741 tgttacgaac aatcatgaat ccttcttctt acttgcttct tgatgaacca tttggagcgt
     7801 tagatgctat gaccagaggg cagatgcaag actggatgtt gaagctgcct aaaaaagcaa
     7861 agcggactac tttacttgtg acgcatgata tcgaagaagc aatttattta tcagacagga
     7921 ttcttgtgct ttccgcccgt ccggcacatg tgattgctga gattcaagtg cctgaaaaac
     7981 gacggtcacg tgaatggcta ctgcagcaat cagagttgaa gcaaacgatt tatcagttat
     8041 tggcaggtga attagatgtt aagtgatgct cattgtcatt tagatgggag tcagagttta
     8101 gcactccttc agcaggaaca cacgattttg acgatcatca attgtgattc gccagaagaa
     8161 tggaaggaaa acagacagct tgcagcaagt aagactcaag ccttaagtta cggtatccat
     8221 ccttggaaag cagatagcta tacgtttgaa caagttgagc cttttttgaa aaaagcaagg
     8281 atcatcggtg aaatagggtt agataatatt tggacgaatg taccgatgac tacacaaaaa
     8341 aaagtatttg agcgacagct tgcttttgca gccataaatg agaaacctgt tgtactgcac
     8401 acaaaaggat gcgaaaaaga gatcttaaat tatatccaag cttaccctaa tcactatttg
     8461 atccattggt attcttcttt ggactatcaa caagactata ttgatttagg ctgttatttc
     8521 agcattggtc ttgatttgaa aaagaatcca gcagtctggc aactagctaa agcagtccca
     8581 atcgaccact tacttattga aacggatgga ctacatgcag cagagtgggc tttagggggg
     8641 attttttcag aaaaagatta tgtctcactg cttgaagaac agttaaagac aatcgctgag
     8701 atcaaagagc tatcagttga agaagttaaa aaaaatactt atcaaaactt ggaacgtttc
     8761 attagattag gtaaataaaa aagagatgtg acacaactag tgtcgcacct ctttttggct
     8821 ttctttgtat aaaagtaaac tgtttgatta ataaatgaat cttagtacca accgtttgct
     8881 tcccagaatg ctttcgccgc ttcccaagaa ccataacgtg aagtaacata ttgttctgct
     8941 actctttctt ggtttgcagc agagtagtca ccatttaagt aagaagaatc taattggtaa
     9001 cgtccgatgt aacgtccgtt cgttgctgtg taagaaccgc ttgattcttt ttgtgcgatc
     9061 cattcttttg cagagcttgt gcttgctgga gtagcagcag tttctgttac agcaggagca
     9121 ggttgtgttt cagcaacagg cgctgcttgt ttttccgttt gaaccgtttc ttgtacaaca
     9181 ggttgttcag cttggactgg tgcttcttga acggctgctt gaggttcagc agctgcttgt
     9241 gcagagcctt ctgttgggat tgttaattgt tgtcctgagt a
//
LOCUS       DAZUKZ010000103         9117 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000103 DAZUKZ010000000
VERSION     DAZUKZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.105"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..849)
                     /locus_tag="ACG8EF_002276"
     CDS             complement(25..849)
                     /locus_tag="ACG8EF_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFS4947563.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(822..1502)
                     /locus_tag="ACG8EF_002277"
     CDS             complement(822..1502)
                     /locus_tag="ACG8EF_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4947564.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1525..2463)
                     /locus_tag="ACG8EF_002278"
     CDS             complement(1525..2463)
                     /locus_tag="ACG8EF_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS4947565.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2708..3559)
                     /gene="ispE"
                     /locus_tag="ACG8EF_002279"
     CDS             complement(2708..3559)
                     /gene="ispE"
                     /locus_tag="ACG8EF_002279"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFS4947566.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3713..6286)
                     /gene="mprF"
                     /locus_tag="ACG8EF_002280"
     CDS             complement(3713..6286)
                     /gene="mprF"
                     /locus_tag="ACG8EF_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS4947567.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6427..7233
                     /gene="proC"
                     /locus_tag="ACG8EF_002281"
     CDS             6427..7233
                     /gene="proC"
                     /locus_tag="ACG8EF_002281"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFS4947568.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7312..8868)
                     /gene="rny"
                     /locus_tag="ACG8EF_002282"
     CDS             complement(7312..8868)
                     /gene="rny"
                     /locus_tag="ACG8EF_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFS4947569.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         2688 a         2016 c         1570 g         2843 t
ORIGIN      
        1 tataaggctt cttttcttta taatttagtt attttttctt ctgaaggcaa ccaatattcg
       61 tttgatgata tttacaatca ggaacaaagc aataaagact aatgtgattg ttgctcctgg
      121 aggtgtatct agataaaacg agctagttag tcctgacagc atccctagaa atcctacaca
      181 aacactgata gcaatcacga cattgaagct tttgcctaat cgcataccga ttgctgcggg
      241 aagaaccatg attgcggata tcaaaagtgc accggcaatc gggatcatca cagcaatagc
      301 cacaccagta atcacattga acatcatcga catcaaatga acgggtagcc catccacgtg
      361 ggcggtatct tcatcaaaag tcagaacata catcggccgt ttgaataaaa agaacaatat
      421 accgatggca acgaataaga ttcctaaaaa gatgacttgc tcccatgtaa tggtcacgat
      481 cgaaccaaac agataggatt ggatacttgt tgctgaattc cctccgctca aattcatcaa
      541 tactaacgct agcgccaaac caccagccat taaaatggca atggagattt ctgaataggt
      601 tcggtagatc gttcgcagat actccaatat cacagctgct aatacaacga ctagcatggt
      661 cgtaagtgta ggattaaggt tcaaaaagaa tcctaacgct acccctgcta aagagacatg
      721 agaaagcgta tcagccatca gcgattgtct tctgatcact aaaaacacac ctagcatcgg
      781 ggcgattccg gcaatgaaga tagcggcaag aaacgctctt ctcatgaatt catatgaaag
      841 caacgccatt gagaatcctc ctttctcacc agtcggatct gtctgtccgc atactgtttg
      901 atatcttcat gatcatgggt gatcattaga actgatttcc catggacatg cgcactatgt
      961 cggagaagac gataaaattc atttctggat tcttcatcca tacctgttgt tggttcatca
     1021 agaacaaata agtcagggtc agtagcaaaa atacgcgcta aactgattcg ctgcttttgt
     1081 ccgccagaca attcacctat tcgccgattt cgcatatccc acatccctac agcttctaaa
     1141 gcacgctgta catgttgatg atctttttca gagagacgct tgaaccagcg gcctcttgga
     1201 tatcttcctg aacggactaa ttcgatcacc gtactaggaa aacctgcatt gaaagatgca
     1261 acttgttgag gaatataacc gatacttaat ttttttcctt cttgattttt ctcagcaatc
     1321 tttatcattc cttttgaagg tttcagtaat cctaacgatg ctttcaccaa ggttgacttg
     1381 gctgctccat tttctccagt taaaatgaca aattcacccg catctacatg ataagagaca
     1441 tcttctagta caggttcatc atcatagtaa aaggtcaaat cagatatttc gatataacgc
     1501 accttacaac ctccattcta aaacttaatt gatactttct tttaaagctt tcagattttt
     1561 ttccatgaca gaaatataat tttcgcctgc tttgatttgt tcattagtca agctttctaa
     1621 aggattcaac acttctagct tcactcctgt ttcattcgac aatgtctcag ctaccttcga
     1681 agaggcattt tcttcaaaat aaatcacttt gatgttgttt ttcttcacaa attcttttag
     1741 ctcagctaag cgacttggtg tgggttcctg atctggtgaa acgcccgaaa tcgctacttg
     1801 ttttaagtcg tattgattcg ctaaataagc aaaagcagca tgctgagtga caaatgtgcg
     1861 gtttgttgca tcttttagct catcggagta tttttgatcc agtgcttcaa gctttttgat
     1921 ataagcatcg gcattcttcg taaaagtttc ctttttatct ggatattttt cgcctaattc
     1981 ttcagcaatc gttctgactt cttttatagc taataccgga tcgagccaaa catgtggatc
     2041 gtattcatgt gaatgaccat gcccttcttc tgattcttca tgttcgtctg cttcttgcga
     2101 ttctaaacgg tcgattcctt tagctgcttc gatcatcaag gtctgtttgc tatccactga
     2161 atcctttaac gaatccacaa aaaattccat gtctgaacta ttatacacga aaacatctgc
     2221 atcggaaatt tctgctcttt cttttgcact tggttcgaaa tcatgcggct ccgttccagc
     2281 agggatcaaa agtttcacgt ttccttcatc ccctacgatc tcttttgtaa aatcatacat
     2341 cggataaaaa gtcgtaacga tcgtcaaatc ctcttttttg tctgcttcct tgttcgtatt
     2401 tccacatgct gcaaacagta aagcacctat cattaccgta gctgtcaaaa gatatttttt
     2461 catttatatg ctcctttcaa tgaacaaatc ggaattttac gatttattaa cgtgaactaa
     2521 tgtatcaaaa taaaaaaaca cagtcaatag aaatccatca atttctgatt taagcatgta
     2581 tattcttgat taaatcgtaa aatggatgag ttgctgctca tatctagcca tttcactaca
     2641 ataaaaaact gaagaatacc attttaggat tcctcagttt ttcatcacct attttacttc
     2701 ttatatttta ttgcagggtt cgtactaaat aaacttcctc acaaaaacct tttaacccat
     2761 tatatactct ctgcgctctt gaataatgtt gacacaaggc aaaaacggtt ggtccgcttc
     2821 cactcatcaa tgcagcatct gctccgtact tgatcatacg ctctttgatt tgatggatga
     2881 ctggatgccg ctttcccgtt atatcttcta aactgtttcc catttttttg atcatctgct
     2941 gataatcttg ctctcggatg gcttcagcaa gtccgtggat gtcttgatgt tggatggctt
     3001 gcgtatctac ttctctaaac actgtacgtg tagagacact cattcttggt ttgaccaaca
     3061 caacccagca ctgtggcata gaaggtaata cttggatttc ttcccctttg ccaccgacaa
     3121 atgccgtact tccgtagaca cagtaaggaa catctgtacc gacttccaaa ccgatttctg
     3181 ccagttcttc taacgaacag cccagattcc ataattggtt gatccctctt agagcagctg
     3241 cacagtctgt acttcctccg cctaatccag ctgctactgg aatattcttc ttaacaaaga
     3301 tcttgacacc ggttttgata ttgtatttat tttttagcaa taaagcagct tgataaacat
     3361 tgttccgttc atctaccggt aaaaatgctt tatttgtttc tacaatgatt ttgtcttctg
     3421 ttatttcttc aaaaaataga tgatctgcca agtctacgct cgacatcacc atttccaact
     3481 catgataccc atcttgccgt ctatataata catccagtcc taaatttatc ttggctggcg
     3541 ctttttcaat caattccatc ctctcaccca ttcgaaaagt ctaagtcgca agtattctat
     3601 catagggaaa aataaaagta tagcttatgt ctgcctagtg gttgattttg tcatcgtttc
     3661 aacagctttc acagaatttt ctcttcataa tccaaacaag ttctagtgat tcttataatt
     3721 ctgtgagcac ttcttctgcc aaactcggaa catttccttt ttcttttcct atcagactga
     3781 gtagctgcat ggtagcaaat ccgaaattac ttcctttcct ataagcaaaa taacgtcctt
     3841 cccaatgatc gacatatttt aatttctttt cataagcaga ctggaaggaa tagaaaaaat
     3901 taccataatt atatacgata tgcatgactt tttcttgaaa gaaagaaaaa tctgtttctc
     3961 caacattagc taatggagct aagcctagat tggcttccga gtagcccatt ttctcgtata
     4021 caataaacag attggctaac agatagtcag ctaattcctc agattcttta ggtaagaccc
     4081 gcaacaaatc atatcccgcc tgcttcttcg tcatcggctg agcggtgatg aagccaacga
     4141 ctttttgatc tttacggaca ataccgacac cgctagataa tacatatgat ttatcaaatc
     4201 gtcccacaga gaagtatttt tctcgtgcac cttgaagcca tttttgtgaa acttcattta
     4261 cagcttgatg taattcttcc ggcagttgat cgtaccaagt aaaatggtat ccttctttca
     4321 tcaaacgttg atattcaagt tgctgactca gccgagacaa gtcttctgtt gctagatcaa
     4381 caatccctgc ttcacctact ttcgtgaagt gaaaacccaa ttcatgaagc atcactacgt
     4441 agctttctga tatcttataa aacgcaagct gatagcctaa acaatctgct tgtttcataa
     4501 actgattcgt tgctgcccgc atcattttag gattgccaac tggatctcct aaaacaaagc
     4561 atttgtttcc tttgatctga taaccaaaaa cgacttgatc ttggtcgtct tcttggtaat
     4621 aaaaataact gtaacctgac aagcgaagct gatggcttgt agctgtgcct ccgtatgttt
     4681 ccaataaacg gtcaaaacgg tctttttcgt actcactgcc aagcttcgta gtatcttcag
     4741 ctaaatattg ataaagcgcc actaaagcta gcaatgagat gcccaaacca ataagccctg
     4801 agaaccacac atcatctgaa ggaaacaaaa taaaatgatt tcctgttatt cgaccgacta
     4861 aaaagcgtcc gctgtgatag cctgcaattg cgtaaacgat aaacaaaaag ccgaacaagc
     4921 tagcatcaaa gattatagct ccccaagaat aaacgaattt tgttcgataa aattcttttc
     4981 tggataacca cacagctaac agaatcaaga catataccaa tatcaactgc caagataacg
     5041 tacgtgtgat tgtatttaaa ataccaaaag atagtaaagc aatcgtaggc agaaatgcct
     5101 ttttgacttt tactgaaagt gctctagcaa ggccgagtaa taaaaagcca accaatagat
     5161 tcactgtctg atctaaaaaa ttgaatgaga acggcaataa tacttgaaat agccgactga
     5221 ttgcagacag attagtgatt gttgatagaa gtaccatcat gatccccgca aaatagacag
     5281 cacttacgag cacgatatgc gcaacctttt ggaaaaacag tcgcggaatg ttatctaaaa
     5341 atcgattgat tttgactcct gtatgtgtga tgaacaatag aagtcccgtc ataaaaggta
     5401 gaacataata aaacaaccga taatataata gccaaactac tgctacatct tgccgaacag
     5461 acaattgact cattcctaag atcatcaaaa cgtcaaaagt ccccattcca cctgggacca
     5521 tcgtcagcat tcctatcaat gtagcaatca tgaacatagg atagatcgaa agcatcgaaa
     5581 tatctattct catcaacttg ccgatgatca aaaataccgc aagggctccc gtccattgtc
     5641 caaaggatgc cagtagcaga ccaagcaagc cttttggata aaactcgaag aacaatgtct
     5701 ttctctttag ataagcaaaa aagaaaatga ctggtgctaa aaaactaccg ccgaaaagcc
     5761 agatccaata gcttcgaaaa atactttctg actgaataaa aaagacatca ataaatgtaa
     5821 gaaacgacca tacagacaaa ccagtcagca taaatagtgc gacttttgat acagtggcta
     5881 aaaccatttt tctatttgtc tgctttccat aaaaactagc ccgcagactt gctcctacca
     5941 caccaccaaa accggcgaga ttgttgatcg tgtttgttac ccacgcagca agaagaaact
     6001 cttttctttc catttttggt tttccttgtt tttctaagac tctgatcgtc acccaatcat
     6061 acaacaacat cggagtgaca cccagcagtc cagccagaat catgagcagg atcgtcatgc
     6121 ggctttgttg tcccatcctt tgccaaatat cttgccaact catgccatta gcaatatttg
     6181 cgacttgatt tgccacaaag actaacacgg agccgaaaaa aatcagtttt aaaatcagac
     6241 tatgttcctt tatccattgc agcatcaagt atcgtttatt tttcacattt tcccattcct
     6301 aacttgtata cttttgtgtc ataccccaat gttatcttat ttattttaat ttttccactt
     6361 tagacctgtt ctttgcttgc gatgatagaa aaagaaagat atcctaatgc taaaggagag
     6421 gataacatga aagtaggatt tatcggcaca gggaatatgg cacaagctat tattgatggt
     6481 ttattaaaaa aaggaacagt ctcgaaagaa gaggtgttcg tttccagtgg acattttgaa
     6541 aatgcacaaa aatttgctgc tgaaactggc gtccatgcat gcaaaagtaa tcaggaagtt
     6601 gcatcacaat cagatatcgt tcttttagcc gtgaaaccaa aaatcattga aaaagtattg
     6661 gaagaactaa gagaatatag ccagaaagtc ttattcattt ctattgcttc aggaaaatca
     6721 ttaatagatt tagaaacagc gcttgaagat cctcaggcct ccatcgtacg tgtgatgccg
     6781 aatgtcaatg tcagcgtagg agcaggcgtt tctgcgattt gcaaaaatga agctgccacg
     6841 gaggaacagc ttcagcaagc tacgatgatt tttgaagcaa tcggcagcgt ctatccttta
     6901 gcagaatcag atttcagtac attcattggc cttgctggca gctcacccgc attcgtttac
     6961 atgctgatcg atgcgatggg acggactggt gtactgaatg gtttgtccaa acagacagcc
     7021 actgagattg ctgcaaaagc aattatgggt tcttgtcaaa aattcctttc aagtgataaa
     7081 aatccatggg aacttgtaga ccaagtttct tctcctggcg gtacaactgt tgcaggtgta
     7141 gttgccttag aagaagcagg attcatccca gcaatcatca aaggaatcaa tacgacgatc
     7201 gagaaagatc ttgagatgtt gaataaaaaa taatgatagc aatgtttttt cgtgccttac
     7261 aaaaaaagaa gagctgtggc ttttgccgcg gctcttcttt tgtatttttt attatttagc
     7321 ataatcagtt gcacgagttt cccgaataac gattactttg atgtgtccag gataatccag
     7381 ttcttcttcg attcgtttac gaatatcacg tactaaacga acagcgtcta aatccgagat
     7441 ttcttctggc ttgaccatta cccgaacttc gcgtccagct tgaactgcgt aacttgattc
     7501 tacaccggcg aagctgttcg aaatattttc cagacttctt aaacgacgga tgtagttttc
     7561 taatgattca cttcgcgcac caggtcttgc tgctgagagc gcatctgctg cagctacaag
     7621 gacagagata acagaagtag cttccacatc tccatgatga gaagcgatcg cgttgattac
     7681 tactgggttt tctttgtact tggcagctaa ttccgcaccg atctcaacgt gagatccttc
     7741 aatctcatgg tcaagtgctt tcccaatatc atggagcaac ccggcacgtt ttgctaattg
     7801 gatatcttcg ccaagctctg ctgctaagat accagctaat tttgcaacct cgaccgaatg
     7861 cttcaagacg ttttgtccat aactagttct gaatcgcaga cgtccgatga tcttgattag
     7921 atctggatga agtgtatgag caccgacttc aaacgctgcc tcttccccgt attcgcgaat
     7981 acgttcatcc atttctttac gagatttctc gaccatttct tcgatacgcg ctggatggat
     8041 tcgtccatct tgtatcagtt tttccaaggt catccgtgcg atttctcttc ggattggatc
     8101 gaatccggaa agaacaactg cttccggtgt atcatcgatg attaaatcga taccagtcag
     8161 cgtctcaagc gttcgaatat tacgaccttc tcgtccaatg atacgacctt tcatttcatc
     8221 attcggaagg gtaacgactg aaacagtcgt ttctgaaact tgatctgctg cgcagcgttg
     8281 aatcgctaat gataataagt ttttcgcttt gcgatctgct tcttctttgg cacgctgttc
     8341 agattctttt accattagag ttaactcatg attcagctct tcttcagtcg atttcatgat
     8401 gacatctcgt gcgtcctctt ttgataaacc agcaattttt tctagttctt tttgttgatc
     8461 ttcaatcatt ttttcaacgt ctttttctcg ctcttcaatt aattgctgtt ttgaactaag
     8521 tgcttcttct ttactttcaa gtgagttctc gcgtttttct aacgagtcat ctttacgatt
     8581 cagcgtttgt tcgcgttgaa tcaaacgatt ctcttgagat tttaattcta acttactttc
     8641 tctcagctca ctttcaactt cagaacggta tttctgattt tcttccttag cttctaacaa
     8701 tgcttctttt tttagagttt ctgcttcttt ttttgcacta tcgatgattc cagcagctga
     8761 tgattgtgca ccagctattt ctttctcgtg acgtgacttt gcgaccataa accctaaacc
     8821 aagaccgaca attaaaccga tgatagcgag gagaatgttg aataccataa aatccacctc
     8881 catactatct ttttcatttt tatcatctat acagcttcta tgataaatta ttctaaactc
     8941 gtcaatatgc atcttcgcat atatgtgtac aaaaaacatg cacaacttta ttctaatgtt
     9001 tgtaccccgt agtgtcaact ttaaataaac aaagaaacgg ctttctactt attcattaaa
     9061 cgatggtttt taaaataaat gacactagtt tcatataggt aaacctttaa attgaga
//
LOCUS       DAZUKZ010000104         8921 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000104 DAZUKZ010000000
VERSION     DAZUKZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.106"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..778
                     /gene="menA"
                     /locus_tag="ACG8EF_002283"
     CDS             <1..778
                     /gene="menA"
                     /locus_tag="ACG8EF_002283"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFS4947570.1"
                     /translation="NYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVI
                     GVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYV
                     NTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDI
                     ENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHL
                     DQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1713..3059)
                     /gene="gor"
                     /locus_tag="ACG8EF_002284"
     CDS             complement(1713..3059)
                     /gene="gor"
                     /locus_tag="ACG8EF_002284"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFS4947571.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3193..4446)
                     /locus_tag="ACG8EF_002285"
     CDS             complement(3193..4446)
                     /locus_tag="ACG8EF_002285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFS4947572.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4452..5375)
                     /locus_tag="ACG8EF_002286"
     CDS             complement(4452..5375)
                     /locus_tag="ACG8EF_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFS4947573.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5596..8088)
                     /locus_tag="ACG8EF_002287"
     CDS             complement(5596..8088)
                     /locus_tag="ACG8EF_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFS4947574.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(8095..8562)
                     /locus_tag="ACG8EF_002288"
     CDS             complement(8095..8562)
                     /locus_tag="ACG8EF_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFS4947575.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8698..8771)
                     /locus_tag="ACG8EF_002289"
     tRNA            complement(8698..8771)
                     /locus_tag="ACG8EF_002289"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8735..8737),aa:Arg,seq:acg)
     gene            complement(8829..8902)
                     /locus_tag="ACG8EF_002290"
     tRNA            complement(8829..8902)
                     /locus_tag="ACG8EF_002290"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8866..8868),aa:Pro,seq:tgg)
BASE COUNT         2479 a         1884 c         1485 g         3073 t
ORIGIN      
        1 taattacatg gattatgaaa aggctcattc tgaagtatat aagtatgaag aaaatgtcat
       61 tggccgcaga aatctatctc ctgtattgat tcgaaacatg attttagcta tgattgcatt
      121 tgtttttgtt atcggcgtta ttctaacgat caatacgggt tggcttttac ttttgatggg
      181 atttatctgt tgttttattg gagtattcta tacattcgga ccgattcctt tatctcgaat
      241 gcctttaggg gaaattttca gtggggtgac tatgggcctt ggaatttttg ccatggtcgt
      301 ttatgtcaat acattcggtt cagatgtcta tgatctgaca gtcaactttt catctggaca
      361 atttgatttg gtaggaaatc tttgggctat agcagcaatc gtattagctt ccttaccctt
      421 ggtgtttacg attgccaata tcatgttggc taataatttg cgggatcttg accgtgatat
      481 tgaaaatcat cgctatacac tagtttttta tatcggacgt cctgcgggaa cagtcttatt
      541 ccagctattg atgtatgctt gctatttcgt gattttaatg gggttgttga gcggtatcta
      601 tgaatggccg atcttggtca ccttcttgac cttgccaatt gtgaagaagc atttggatca
      661 attcaagcaa acgttacccc agcctatcag tttcagctat tccatcaaaa atatggtctt
      721 attcaatgcc agttatgtat tggggctatt actaacaatc attatgcaag ccatttaaca
      781 gcatgaagag gttgtgaaaa aactgccctg catcaagtaa taagaacagc caaataaaaa
      841 tagctcctta tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac
      901 cgtttatcag gaggttgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
      961 aaatggcttt ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac
     1021 accgtttatc agaggttgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg
     1081 aaaatggctt tccacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa
     1141 caccgtttat cagaggttgt aaaatcagtg ttttgtcctg agtattagag aaacaaatgg
     1201 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgaaggaca gctgattgaa
     1261 cactatttgt tcggaaaaag tgactaacta tgacacaatt tttttcacag tccctttttt
     1321 cacttgattt gattcgtacc agcgaacttg acgagaactt tcttggtttg gtaactatat
     1381 ggtattcttt taacatcctc gtttgcttct tctaatttat ttatacaaag tcacactttt
     1441 ttaaacatct gttcttcgat acatttctat ttcttttaaa catcgatacc aatattttga
     1501 acttaagagt gcataccaaa tggattcatt tactcttcgt cagttacgaa gataatcaaa
     1561 atctccaact tccctactta tttcgttttg agaattatcg aagcaatctt acataaataa
     1621 ctaaaaaaca aaaaaccaga aaatcagtat actgattttc tggttttaca tggtttgttc
     1681 aataaaaagc tgttggaact gttaatatta aattatcgca tcgttacgaa ttcttccgct
     1741 cctgttggat ggatggctac tgtattgtca aagtcttctt tagttgcacc catcttgata
     1801 gccacggcaa aaccttgcag catctcatcg acaccaacac caatagcatg taagccgacg
     1861 attctttctt cttttccgac acagatcaat ttcatttcgc atttttggcg ataatcattt
     1921 aatgcaaagt acattggggt aaagcgtgaa cgataaactt tcaattcatt ttcgccatat
     1981 ttttccaatg cttcttcttc tgtcaaacca attgttgcaa ttggtggatg cgtaaagacg
     2041 actgtcggaa caagtttata gtcaagataa gaatctttct ttccattaaa taaacgttct
     2101 gataaacgtc taccagctgc gattgcaaca ggagtcagat ctaatttacc gatcacatca
     2161 ccaacagcaa aaatatgttc atccgttgta ttttggtatt tatctacttt gataaaccct
     2221 tttttgtcca attccacatt tgtattttcc agacctaatt tttcggtatt cggtacacgt
     2281 ccaccggcaa aaagtacaca atcaccagtt aaggtttcac cattttcaaa tagcaccgta
     2341 tattcgtcat tgtttttagt aatttcttta gcaatatgat gtgcgtaagt ttttattcca
     2401 gcttcttggt acatttggat caaattgtcc gaaagcattt tatcaaacgt tctaagtggt
     2461 cgttcttttc gatatgccca catgacttca ctacctaaac cacgaaatac acctgaaagt
     2521 tcagccgcaa tgtatccagc acctaaaacg attgctctct taggtaactc tgttaattca
     2581 aaaaatccat ttgaatcaat tgcgtattct ccaccaggaa tggtcatttt acttgcacgg
     2641 cctccagtag cgatcaagat tttaggagca cgataggttt caccgtttac ttcgatcgtt
     2701 tgactatcga taaaagtagc gtaacctttt atagcctcga catgattgct atctaatcct
     2761 ctttgataag caccgtgtag aaaatcaata tatttttctc gtctttctac tagaccggcg
     2821 aaatcaacat tttttaaatc tgcttggata ccgtaacttg aggcatctcg ttcaattgtt
     2881 tccaaaatcg tacttgcttg ccacataact tttttaggta cacaaccgac gttgacacat
     2941 gttccgccca attcattcgc ttcaatcagc aaaacttttg cgccatgcat ccctgctctg
     3001 tttgctgaag caataccgcc agagccgcca ccaattacga tgtaatcata tatttgcatt
     3061 ttgttcctcc ttattgaaga gatcaatcaa aacctttatt taacgatcct ttttctacag
     3121 aaaacaagtg taacactttt cttgaatttt gtatgcgaag atgcttaaaa cagggatata
     3181 tcagcttttt ccttatttac gttttcggat catatctcca ggtttcaccg gctgtgcgac
     3241 tggcatagtt aaaatcatca ttggatttgg tgctcggtca atggattcgc cttcttcatt
     3301 atgcataact tcaacggttt gactgaaatg actgaatcct ggaccataaa attcgatttc
     3361 atctcctaca gaaaacaagt ttctctgacg tatcgtagca atctgtgttg cctcatcata
     3421 agataatact tcaccgacaa atttatatac tgggatttta cgacgttcac cgaataattg
     3481 ttcgttttca gttggtgtat tgtagtaaaa acctgtagct aattctcgtt gtgctacttt
     3541 ccataactcg tcgatccatt cttgcttgca gacatagttt tcaggatctt ccatataact
     3601 gtctacagcc gctttataga catttgacac cgtagataca taatggatcg atttcattct
     3661 tccttcgatc ttgaagctgt ctacgccgtt ttcaatcaat tctggaatat gttcgatcat
     3721 tgacatatcc actgcactca tcgagaattc ttcttccact tcgcctgtat cagttaaact
     3781 gtttcgttct tgaccaaaag gcatatcgaa caatccatac ttccagcgac aagattgaga
     3841 acatccgcca cggtttgcat cccgcatcga catatggttg gacaatgtgc atcgaccaga
     3901 ataagagata cacatggcac catggataaa tgcttcgatt tcggtatccg tatttttacg
     3961 aatctctgcc acttcctcca ttgaaacttc acgagctaaa acgacacgtt ccaacccttc
     4021 ttctttccag aattccaatg tctcaaaatt agttgctgaa gcttgagtgg ataaatggat
     4081 cggcagacct ggggcttctg tcgcacaaat ttcgatcaaa gctggatcag aaacaatcac
     4141 tgcagaaata ccgacatcac gcaaactgcg gaaaaattcc cctgctccat cttcatttcc
     4201 ttcatgggtg accatattag cagcaacgta taccttcgca tcatgttctt ttgcaaaagc
     4261 aactccttca gccatttctt cataagtaaa attacctgca cggcttctta aaccatatgc
     4321 attcccgcca atatatactg catctgctcc ataatggatc gcggttttta acttttccag
     4381 tgttcctgct ggcgctagga tttctggtcg tttcaatttt ctttttactg tcatttcttt
     4441 catcatactc cttatttgat agcagaagga tcaatataaa agaatcctgt atctaattca
     4501 cggttttcag gatgcaattt actgatttcg ctagtcattt tttccgcttt ttcttgatcc
     4561 cagctacctt ctaaaatttg tttttttgct tcggcgaata gtcgagtgat gtttacgaat
     4621 ttttcgcctg gagtaaataa tccgtccaat ttccatgtac agtaatgatg ctgataaagt
     4681 tcattcaatt cattcgagag attcagatca ttgctggcaa aaatatgtgt gccatgacta
     4741 tcttcataaa tagaataatg tgtctcttct tttttaggtt ccgagataaa aagacctctt
     4801 tcacgatctt tttgttcgtc ttgtttcgta tagttatagt aattctgtag cagtggacgt
     4861 ttggattgat ggatgcatgt agcaccatac acaagggttt ctacaggaat atcgagtttt
     4921 tcttccatcg ccttcatctc ttcaaaagga acttcacgag caagtactgc tccaactgcg
     4981 ccttttttcg cccagaaatt gatttgacgt gcgctcgtta ctaatgtttc agcatcataa
     5041 atatacggta tacgcaattc aggatttttt ttcatcgtat aaataatacc tgtatcacca
     5101 actgtgatca catctacgtt tatttctttt aaaaaagcca aatattcagg tacatttttc
     5161 attttttcag gatgaatgat cccatttaca gcaatattcg cttttttacc atacttgtgt
     5221 gctaattcta ccaattgtcg ctgttcttct cgtgtaaatg aagcaggcag acgcaatcca
     5281 aattgctctt caccgaaata tagtgtatct gcacctgcag caagcaactg ttctgcttgt
     5341 tcaatagttt ctactgtggt tatgagttcg atcataattt tctcctccta caacttcata
     5401 atagtagcat agctctaaaa actttccaat acttcttcta tttaaagtcg gatttttgaa
     5461 aattatttca caaaaaatca aaataaagtt cgatatattc tgtccaaata taaaaagaga
     5521 ttgtgacaaa aatcttgtca caacctctta atcaagtctt actattataa aaattcagct
     5581 acacaggcca agtaactata gggaacctac tgtttccttt ttaggtccgc gaacattcaa
     5641 tgtgatctta ccttttgaag cacctactgt tacgtggtca cctaaacgaa tttctccgcc
     5701 aagcaaagcc tcgctcaatc gatcttcaat ttctttttga agtgcgcgac ggattggacg
     5761 tgcaccatat tcaggatcaa atccagcttt cccgatcaca tcgattgcag atggtgtgat
     5821 tttcacttga atatcttgtt ctttcaatcg tttgataatc gctttgctca tgatttggac
     5881 gattgtatgg atttcatctt tactcaatga atggaagacg acagtctcat cgattcgatt
     5941 caagaattca ggtcggaaag ctttctttaa ctcttctaag atacgttttt gcatggcttt
     6001 gtgatcttta gtaacatctt ggacattgaa tccgacattt ttttcttctc gaatttgtgt
     6061 cgctccgata tttgaagtca taatcatgat tgtatttcta aagtccactt ttctaccttt
     6121 agaatctgtt aaatgaccat catccaatac ttgcagaagt aaattgaaaa cgtctggatg
     6181 tgctttctcc acttcatcta agagaatcac tgaatatggt ttttgacgaa ttttttctgt
     6241 caactgtcca ccttcatcat accctacgta tcctggagga gacccgatta atcggcttgt
     6301 actgtatttt tccataaatt ctgacatatc aacacggatc agggcatctt cacttccaaa
     6361 catgacttct gaaagtgctt tggccaattc tgtttttccg acaccagttg gtccaaggaa
     6421 catgaatgaa ccaattggac gatcaggatc tttcaatccg cttcgtgcac ggcggatcgc
     6481 acgtgaaact gctttgacag cctcatcttg accaacgaca cgttcatgca gaagtccttc
     6541 cagttctaac aagcgttcgc tttctttttt ctccaactgc tgtagaggga caccagtcca
     6601 ctccgataca actgtagcaa catcttcttc tgttacacga tcagaataac cttgctcctg
     6661 ttttgcttca attgcagaaa ctttttgcag ctttttacgt agtgcttttt cttgtctgcg
     6721 taattgagca gcattttcaa agtcttgttt ttggatcgct gcttcctttt cttgtactag
     6781 ttcatcgatt tctagtttga tcacagttga ttttgttaag tgatcggttt gatccaaacg
     6841 gactttcgct gctgattcat cgatcaaatc aattgcttta tcgggtagtt gacgagaagt
     6901 gatataacga acagaaagat tgactgctgc tcgtacagct tcatctgtga tctccactcc
     6961 atggtgctct tcatatcttg tacgtaatcc ttgaagaatc acttctgctt cttcaggagt
     7021 tggctcatct acttgaatgc gagcaaaacg tcgctctaat gcagaatctt tttcgatata
     7081 tttttgatat tcatctaggg tcgttgctcc gatcgtttgt aattctcctc gagctaacgc
     7141 cggtttcaag atattcgaag catcaattgc tccttctgct ccacctgcac caattaaggt
     7201 atgcaattca tcaatgaata agattacttg accatcatta tagatttcgt cgatgacctt
     7261 tttcattcga tcttcaaatt caccgcgata tttcgtacca gcaaccaaag atcccatatc
     7321 aagcatcatc aggcgctttt cttgcatgtc ttctggcact tcacctaaaa tgattttttg
     7381 agctaaccct tcagcaatgg ctgttttgcc tacaccaggt tcacccacca gtacagggtt
     7441 attctttgtc cgtcggctaa gaatctggat aagccgtttc acttctctcg aacgaccaac
     7501 aactgggtcc agcttgtttt cttttgctaa tttcgtcaaa tcacgtgcta atgaatctag
     7561 cgtaggtgtt ccttgcggtg tttgccgttt atcttgtaca tttacatttc tacgacgatt
     7621 cattcctttt ccagcagaat caccagctag ccccattttt tgtttcagaa cttgacgggc
     7681 ttttgaaagg ctgataccta agttgatcat gattcgagta gccatgatac tttcatcttt
     7741 taataagctc atcaaaatat gttctgttcc aactttttga gaaccagttt tcttcacttc
     7801 gctgctagct aaagcaaaaa cttgtttcat tcttggtgaa tatggaagaa agactccttt
     7861 agggtaagaa cggataccac cataaccaat cagatgttca atttcttcat aaatttccgt
     7921 ttcattcaaa tccatttcac gtaatacttt tccagcaatt ccattttgct cgaccactaa
     7981 ggctagtaaa acatgttctg aactaactga ttgatgtttc aaccgttttg cttcttcttg
     8041 tgcaatttct aatactgctt gggctctttc agtaaaaagt tcttccatct aatctcactt
     8101 cccatcttca taacttaaac gttccagaaa tgctcggaga atccgagcac gtgtatgatc
     8161 ttcatattca ttgaaattca atgtgttttt tgcaattgca ctcaacatca agtttccttc
     8221 tttttttgta attatttggt cttcataaag tttttgaata atagcaaaag catttttctc
     8281 actgattgta tcatcaaata actgatttat ctgctcaagt aattgttttt tatctgaaat
     8341 ccttactttt gcaattcgta tataaccacc accaccacgt ttgctttcta ctagataacc
     8401 acgttggatg gtaaaacggg tattaatcac atagttgatt tgtgaaggaa cacagttaaa
     8461 gaggtctgcc atttcaatcc ttcgaatctc gatcatttca ctattttcca gaattttctt
     8521 tatatatgat tcaattaaat ccgaggtatt ctgatgtgcc atcttattca ttccctttga
     8581 ctaatattga ccttaattat aacgaatttg ataatagaaa gaaagaagaa cgctttttgt
     8641 taaataaatc ctttagaatc aacaaaaaaa aatacggaat atactattcc gtatcgacgc
     8701 gcccaagagg agtcgaaccc ctaacctttt gatccgtagt caaacactct atccagttga
     8761 gctatgggcg catattcaat ttaaaaaaat tataaatcat ttgaaaacaa aaagcaagat
     8821 aaaaaaaatc gggaagacag gattcgaacc tgcgacccct tggtcccaaa ccaagtgctc
     8881 taccaagctg agctacttcc cgtttaaaaa ataaaaaaga c
//
LOCUS       DAZUKZ010000105         8866 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000105 DAZUKZ010000000
VERSION     DAZUKZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.107"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..1292
                     /locus_tag="ACG8EF_002291"
                     /pseudo
     CDS             78..1292
                     /locus_tag="ACG8EF_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1471..1806)
                     /locus_tag="ACG8EF_002292"
     CDS             complement(1471..1806)
                     /locus_tag="ACG8EF_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4947576.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1790..2053)
                     /locus_tag="ACG8EF_002293"
     CDS             complement(1790..2053)
                     /locus_tag="ACG8EF_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS4947577.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2513..3316)
                     /locus_tag="ACG8EF_002294"
     CDS             complement(2513..3316)
                     /locus_tag="ACG8EF_002294"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4947578.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGTFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3294..4418)
                     /locus_tag="ACG8EF_002295"
     CDS             complement(3294..4418)
                     /locus_tag="ACG8EF_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4947579.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4434..5249)
                     /locus_tag="ACG8EF_002296"
     CDS             complement(4434..5249)
                     /locus_tag="ACG8EF_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4947580.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5239..6111)
                     /locus_tag="ACG8EF_002297"
     CDS             complement(5239..6111)
                     /locus_tag="ACG8EF_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4947581.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6196..7485)
                     /locus_tag="ACG8EF_002298"
     CDS             complement(6196..7485)
                     /locus_tag="ACG8EF_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4947582.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7629..8162)
                     /locus_tag="ACG8EF_002299"
     CDS             complement(7629..8162)
                     /locus_tag="ACG8EF_002299"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947583.1"
                     /translation="MANEKATMTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQL
                     SWAAIWGKHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAR
                     EVGQQLGLTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGV
                     KRFGDGIHDHMGALFLA"
     gene            complement(8208..8351)
                     /locus_tag="ACG8EF_002300"
     CDS             complement(8208..8351)
                     /locus_tag="ACG8EF_002300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS4947584.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
BASE COUNT         2750 a         1883 c         1413 g         2820 t
ORIGIN      
        1 aaaatattat agaagcaaaa tcaattctac cttagatgtt cttatatcac tctatcagtc
       61 ctatttgaca aagagccaag aaaaagacgt tggtcgtcga tgctgttctc tcgcctactc
      121 ctcgtgcttg tagaagttgt ggttctactg tggtagatgg aaacgggaaa gcaattatag
      181 tgaaaaatgg gaagaaggaa acgattgtcc gttttgaaca atacaatcat atgcctttgg
      241 ttacgcgcct aaaaaagcaa cgctatacct gtaaaaactg tcgtacccat tggacggctc
      301 aaagttattt tgtccgactc agccattcaa tcgcagatca tgttcgatat aaaattgctt
      361 ctttactgac tgaaaaagta tctttgtctt ttattgcgaa aagttgtcag gtatctttga
      421 ctaccgttac tcgtacattg aaagagttta aaagctattt accaaagcct tctaagaaga
      481 ttctccccag agtattgatg gttgatgaat ttcgttcgca cgcttccata gaagataaaa
      541 tgagctttat ttgcgcagat ggtgaaacag gaaacttaat tgctgtcttg cctacgcgta
      601 aattacctcg attaacaagc tatttcttag gctgcaccaa tccagaagaa gtagaattct
      661 tagtcacaga catgaacgcc gcttacttcc agcttaccaa acgtgttctg ccaaatgcga
      721 aagtagtgat tgatcggttt catattgtca aacacatgaa tcaggcactc aatgagttgc
      781 gtatccgtga aatgaatgag cttcgtaaag caggacagaa aagtcaggca gaaaaactga
      841 aaaagaactg gcgctttctg ctaaaaaatc gtgcaagcat caaccattat gagtacaaaa
      901 catggaaaag tttccgagca ccaaaatacc catttctcac tgaagcaatg atgattgatc
      961 gattgcttga gttttctgcg cctctaaagg aggcgtatcc cttttttcat gaattagtcg
     1021 aagcctttcg agacaaagac cctgacttat ttttctcctt attggcagaa cttcccgaaa
     1081 cgttagatga cggctttcgg gagaagcttc aaaaccttct gacctatgaa gaaggtatca
     1141 ccagcgcaat gatctatcct tattccaatg gaaaaattga agcgaagaat acccacataa
     1201 agacaatgaa acgagtatcc tacggattta aatcattcga gaacatgaga attagaatct
     1261 ttttgatcaa tcaattaatc aatgtaagat aacaaaaaat ctgaaccaga atgtggttca
     1321 ttctgacccg gatttgattt tgcacaactc atcagtcctt attgacaaag agccaaaaat
     1381 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
     1441 attgacaaag agcccttctt tttagtatta ttataattct tccatgcgtt cttccagtcg
     1501 ctgtaaatcg cttggactta attccccaat ttttttccat ttgacctttt tgttgttttt
     1561 gggtaattcg tttagagttc ctacgtcaat ccaagattct ttttttagtc ctgcctgttt
     1621 ccaatccctt atttcaacgt atttcagctt tattttatcg gatttagtat gatattttga
     1681 agtaattgac agaaacgaaa ttgtgtccag tccatttttt agaatataga caggccggcg
     1741 tttaccgccc tctgcagctg tgaaagcaac gtagacggtt gctatatcat tagtattcat
     1801 cttcattgaa ccattcttca attttttcgt cagtgtctag tttttggaca ggcaattttt
     1861 ccgctaatct ttgaatatta tggattcgtt tacttttttc cgacaattca atagaaaatg
     1921 ggatttttcc ttgctcaaca atttgcttat aaaaaacggt gacggctgtt ttggggctca
     1981 tgcctagttc atcaataatg ctttctgctt catttttcaa tttttcgtcc atagaaattt
     2041 gtaacttagc cattttctcc acctctttct gacaaacaca ttatatcacg gaaagagttc
     2101 aaatggaatt aatacaacta cttttgaact ccttgaaatg cagtgtattt atctaaatat
     2161 aaggaaagga tatagtgcta aatggccttg atagatagaa aagtttcaaa atccgccaca
     2221 gcgataattg aagcgaacac tcaacgacac acagaacagg aaaagactcc taaaagaacc
     2281 aaattaacta ggacgtgtct gaaaactcca caaagatgtg gtatgctctg gtaaaaaacg
     2341 aatggcacaa ccacgaagac cgatatggtt cttggaaaac tttctccacc ctttttgtaa
     2401 ttggagggat agtggtttat tgatagcgat tttccaagtt cttcaagtgg aaccagacat
     2461 tgagcaccac ggctaaaaag tgaatgaagt ctttattttt aatcaataat ttttaatgat
     2521 taccagtaat tggttttttc atgcgcccta tttctatttg atgtggagtt aaataatgct
     2581 gataagtaaa aatttttccg tcaattaaac tgccactagt gaatcctgtt ctattggtaa
     2641 aaagagaata gtctgggtat tcatctacac caaatgataa agattcagcc ataaactgtt
     2701 ttttaccagc aaattcagca ctgctacctt gatggatatg tcctacaaaa gtacctaaaa
     2761 tatcactttc ggcaatcaat tcagcaaaac cttccggaac ttcagttaca atagatggat
     2821 cttcccacga caatggatga tgaaccaata aaatcgttcc cttttcagca ggtgtaccca
     2881 accactcttg taattgaatc atctgtccta tcgatatttt tccttcgtgc tgggtatctt
     2941 gagcagaatc caaggaaata attcgtaacc cattttgatt cgtacaatgg atatactctc
     3001 ccgtttcatt gctttcaaca ctcattcctt caaagaaaac actgcgacga tcatgattcc
     3061 cacgacaaaa atagtaaggt gtggttggga aatattgatc caacattttc cgtaagactt
     3121 gataatcaga aatcccccct tcatgtacta aatcacctgt taatagaata aaatcgtaca
     3181 ctgttaacga gagttcttgt aacaattttt ccaatgccaa ctgtggattt ttaattgttg
     3241 gaaatagttc agcagtaaac aagttagttt gatagttttc tctaaaatgg atatcagaga
     3301 gatgcaaaaa gcgcatggct attccctctt tcttcaataa aataatcagg atatcctaaa
     3361 ttttcttgcg tctgataatc aaaaaaatgc attctttcag gttctatgcg gagattgact
     3421 gcatcaccaa tctgccaagt aggtttttca ttaaggacaa tcatttctat accatcgact
     3481 ttaacggtgt acgcataact ttgacctaat agctcacgat acttcacttg accgtgtaaa
     3541 tcgcttgttt cttttgaaaa ggttacgtgt tctggtctta tgcctaaata caatgagcca
     3601 ctaacctgaa gaagttgttt ttgccaagct ggattcaatg ttaaaaattg actggctacc
     3661 tttaatgttc cttgatcata agtaactggc ataatattca tgcctggcga accaataaaa
     3721 tttgcagtaa aagtattttt aggtttcgta taaacatttt tcggcgtatc aatcatctga
     3781 attctacctt cattcaataa aacaatccgg tcagctaacg tcatcgcttc aatttgatca
     3841 tgagtgacgt agacaaaagt ttgtttataa cgttgatgaa tttttaccaa ttcttttcgt
     3901 gcatccaatc gtaaacgcac atccaaattt gataacggtt catctaataa aaaataatta
     3961 ctccgtttaa ccaccgcacg agccaatgct acacgctgcc gttgccctcc tgacaaatct
     4021 ttaggaagtc gatcttggta tggcgttaaa cctaacatct tcaaagcttc ttccaaacgg
     4081 tgctgaatgt cagctgtctt aattttattc gcccttaatg caaaaataat attctccttt
     4141 acagtcatgt gaggatataa agcataattt tgaaaaacca ttgcgatctc tcgttctcca
     4201 caaggtagct tatttgctag ttgtgtacct aaaaaaagct ctccactcgt aattttttcc
     4261 aaaccagcaa tcattctcag caaagtagat tttcctgaac cagatggtcc cagtaacaca
     4321 attcgttctc cagcggccac agctaaatca atttctttta aaatggtatg ttctccatat
     4381 ttttttccga tatttcttaa ttcaagcgtt tctcccatga tttgtcctcc aatttattta
     4441 atacctgcag aaacaaaagt cccgataata tatttttgaa aaattgcata caagactaac
     4501 ggtacaaaca ttgtaattaa actaatcgcc attgctacgg taaaatttgt cccaccttca
     4561 gaactaatat aggtctgcat cgccaacggc aaagtataat tatctactgt ccgtaaaatt
     4621 aataccggcc aaacatactc attccaagag gtaataaaaa accaaatacc ggtagaagta
     4681 atcgcatgtc tagtaagagg aaaaacaatt ttactcatga cggttgtttg tttgatgtta
     4741 tccaatgccg ccacatctag aaggctcact ggaatttccc gcattgtttg cgtcaataaa
     4801 aaaatgccca tcgcatcagc aaattgtggt attatcacac cccaagtagt atcaattaaa
     4861 ttaagttttg caatcaacaa ataattagga atcatcgcaa cagtaaaagg aatgaacatc
     4921 gtaccaatga tgataaagta ccagattttc ttccctttga ttcggcaata agtaaaacta
     4981 tatgccgcaa aaaaagctac gcctaactta acaaccgtta taattgtagc aataaaaaaa
     5041 gtatttctcg taattttgat aattggaaga ttttcgaaca aatagcaaaa attttccaag
     5101 gtaaacggct gtggtatcaa actagtaatc gtctgatagg catctttcaa tggcttaaaa
     5161 gaattggcca taataaacaa taatggatag ataataatga ttatgatgaa cataaataag
     5221 agatgtggca accagcgttt aattttcata gtaaaccccc ttttccacaa atttaaatat
     5281 tacccctaag aaaacagcaa atattaataa agttaaaact gaaattgctg aagattctcc
     5341 gactttatac atttcaaaag ccttttgata agcataatag atcacgtttg cactaccatt
     5401 attcggtcca ccacgagtga tgatcttaat cggagtaaac acatactgca gaccttgaac
     5461 aatggtcatc accaataaat atagtcctgt cgaactactc ataggcaaca caattgtaaa
     5521 aaaaatgcgc cagatgggta cacgatccaa cttagctgct tcgatcatct cttgatcaat
     5581 tcccacaatt gctgcaaaaa tgacaatgaa attataacca aaaactttcc aagcagtaat
     5641 acctgcaatc acagcaatta cccaaccttg taaattcatc cacataggca gtcttaaacc
     5701 aaaatttccc aacaagacag caattggtcc cgataccgga tttaatatcc atgtaaaaac
     5761 catccccgcc actaccaaag aaatcacagc tggaataaaa aataatggtt tatacaaatt
     5821 ctgccctttt tctataacta ttcctaataa aaaggatgta atatatggga acacacagct
     5881 cagcaagaca aatagaacgg tataggacaa agtattcttc aacactttat gaaagactgg
     5941 atcagtcagc acctttacat agttggcaaa gccaacaaat tctttagtgg gggccaccat
     6001 attccagtca aaaaaactca aatagatcgt ataaatcaat ggataaaaaa caaacacgcc
     6061 caacaaaata attgctggcg ccagatacaa ataacctaca tagcgcttca tttccgacag
     6121 cctcctcatc cttatgtcaa aagcagacga ccttgttttt cctttcttac aggcgctgtc
     6181 gtctgcttgc tttgcttatt tgagtaactc attaatttcc ttttgagtag ctttcatcgt
     6241 ttcattgacg gaattacctg ataaaatttt atcccgcata tccaacaatt tttgttcagc
     6301 ttctaatcca gcatctccag gaaatgaact ccaactgacc atcttatcca tctgatcaat
     6361 tgctggtatc atcatctgat tttcagctaa gaaagctttt aatccatttt gggcgtctgc
     6421 aacatcttta cgtggtggta aataaccagt accttctgtc catttagcta aagattcaac
     6481 actataaaga tattttaaga aatcccaagt tgcttccttt ttcttatccg tttgtgatgt
     6541 aacagctaac attgcgccac cagcaggtag tttaactttt ttcccttccc aacttggtga
     6601 ctcaactgct ttagcagtaa aagtactatt tttttgaatg ttgtcccttt gagcaatagt
     6661 ggtatagacc atcgcgacgt taccatcaat aaagctttgc acaccttgtt cccacgtagt
     6721 gtgtaatgcc gtctggtctt ttaccatcct ttgatataat tcataagcag cgataccatc
     6781 tttagaagca aaggtagctt tgccatcttt tattacactt gccccattac tttctaaaat
     6841 tgcttgttgt gcccaattat cagcaggttc ttgaatatac acgccatact ttccagtttt
     6901 ttcttttatt atcttagaaa aagtttgaac ttcttcccaa gtttttggac cattttcatc
     6961 caagcccgct gcttttaata tatcttgatt taaatataaa accggattac taatggagta
     7021 aggaataccc acttgtttac catctttttc agacagtgcc atgatattgt ctaaaaaatg
     7081 atctttaaaa taatcagcat tatccggatc cacttcttct actaaagatt gaggattagt
     7141 atatttgaaa ttttctgcaa aataatcttt aaaagcccat cccacttgta ctacgtccgg
     7201 tatttctttg gaggcaacag aagcttgtaa attttgcatc aagcctttat acatatcagg
     7261 attatatttt gctacaacct tcactttgtc ttgagattga ttgtatttat ctactaattc
     7321 attcactgtt tttccaccta gggtatccgc attaacatgc caatactcta tcgttacacg
     7381 accctccgat ttttttgttg tcttggtatt cccactacca caagctgcca aagagatgac
     7441 acttaaaaca gacacaaccc ctataacaag atttttcttt aacataacat taacttcctc
     7501 ctaatttctt tatggccatt tttctaatca agaaatatta tgaactacca atgtaaattt
     7561 ctagttaatt ttctgtaaat aattcgaaat ttgtgcattt cggggtacag ggtcactgga
     7621 catatccatc aagctaagaa taaagccccc atatggtcat gtatcccatc tccaaatctc
     7681 ttaactcctc tgttttgatt ataaaatagt ttattctgtg ctcctttctt ccataaatag
     7741 gcccatgcac aacgtgaggt tatattataa acaccacagc gcgcaaggta ctcaatcgtt
     7801 tctagttcat tgactgtcca tacagaaaat gtcaatccta gctgctgccc cacttcacgg
     7861 gccaacttag ttaaccatcc ttcatgaata ttgacgattt gaacgctttg ataggctaat
     7921 ttctgtaagg tatcgatcaa atctgcttcg gtaatatccc caagtttttt cccttcgaac
     7981 tgattttcaa tattataaaa tatatgcttt ccccaaatcg cagcccaaga taattgtaca
     8041 tagccactaa gaacaatttg tggccagatt tgataggttt gtgccagcga aaaaacggcc
     8101 atttccaatc cctcttcttt taaatcacag ttaatggtca tggttgcctt ctcattggcc
     8161 actgcttgaa acacctgctc aagggttacc gaatctggga caacttctta cctgtcatgg
     8221 gctagtatta gcgtaccatc tgtttgctga cgcacatcta cttcaaaaca aagctggcga
     8281 tccaaacaaa agcgaagaaa tgcccagcta ttctcctccg tattgtcact accagagtgt
     8341 gcagtgatca tggtcatttt gacccctcct ttaattccgt gattttctcc cacacgtcca
     8401 ctataccgat gaagcgttaa tttcttgtta atttcttgta aattccctgc gttttttcag
     8461 taaactagtt gtaaatcttg catgctcctt cctacgaact catgaatcgt cgattttgcc
     8521 cttctagcta gtttcaccaa ttaagtagtt ctacattccc cgcttttaac ctactacacc
     8581 tttttttgtt ttcagctatt ccttcttctt gctgaaagtc cttgaggttc tttcttacag
     8641 gataccatca aggtgttcaa tggcttattc ccattgaatg accgagactt actatcagat
     8701 ttctgctcgt tgaaccaagt ctttgataca ggatttcttc cgtcatcttc tcacaaatac
     8761 caccttgcgt ttctctagcg attggtcgat gtgcaccctc ccagtggact gtcactaccc
     8821 ctttttgcta tgcctagccc acaaaaaaag ctgtcctcac agacag
//
LOCUS       DAZUKZ010000106         8841 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000106 DAZUKZ010000000
VERSION     DAZUKZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.108"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..883)
                     /locus_tag="ACG8EF_002301"
     CDS             complement(<1..883)
                     /locus_tag="ACG8EF_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HFS4947585.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLN"
     gene            complement(889..1995)
                     /locus_tag="ACG8EF_002302"
     CDS             complement(889..1995)
                     /locus_tag="ACG8EF_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFS4947586.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            complement(1995..3131)
                     /locus_tag="ACG8EF_002303"
     CDS             complement(1995..3131)
                     /locus_tag="ACG8EF_002303"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4947587.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(3163..4614)
                     /locus_tag="ACG8EF_002304"
     CDS             complement(3163..4614)
                     /locus_tag="ACG8EF_002304"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS4947588.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(4658..5422)
                     /locus_tag="ACG8EF_002305"
     CDS             complement(4658..5422)
                     /locus_tag="ACG8EF_002305"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFS4947589.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(5450..6148)
                     /locus_tag="ACG8EF_002306"
     CDS             complement(5450..6148)
                     /locus_tag="ACG8EF_002306"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFS4947590.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(6160..6939)
                     /locus_tag="ACG8EF_002307"
     CDS             complement(6160..6939)
                     /locus_tag="ACG8EF_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFS4947591.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(6955..7899)
                     /locus_tag="ACG8EF_002308"
     CDS             complement(6955..7899)
                     /locus_tag="ACG8EF_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4947592.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7945..8724)
                     /locus_tag="ACG8EF_002309"
     CDS             complement(7945..8724)
                     /locus_tag="ACG8EF_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947593.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2505 a         1842 c         1313 g         3181 t
ORIGIN      
        1 tatttaaatt tttaatcaat tcgtcacata atggattgtt actaggtatt ttatcaaatg
       61 tctgtgctat ttcatgattt attctctgtg ctaaaacaat catataatct atcatcaaat
      121 aatccaccat catatcatta tttttccaat aatttaaaaa aaagtctcta attatagcaa
      181 acatccatct agactcttga ttacatgcta atgaataacc cgcaaaatat cctgatgcta
      241 cggatgcgtg agcataatca ggcctcctaa tagaccaaat atcgatatca aaatactcat
      301 ctggtattgg ttttgtacaa taaaatgtgg cgtctaacca caaacctcca tgttttgcta
      361 gcaaagataa tcttaataaa tcagaataat ttgttctcgt aatgattcct ttgtttttct
      421 tttcttccac ccattttggt atatcaacat attgtctata attatcttct gttaaaatta
      481 taacagtatg tttccctgca tttcgtttta ttgattttac acaagctttt acaatttccg
      541 gcgattgatc caatccctgc caccaacaaa cccaaatgca gttagaatat ggtgattttg
      601 attgattaat tatttgattt tgactataat catatgtttt caaaaaatct gaaaaagtct
      661 tttcataata cttcagcatt acctcatgtt tttttaataa atgttccttc tgttttttac
      721 tttcattata tccattacga ttcatgactt gaatatcaat tttagcttgt aacgtggcaa
      781 atgcttcctt ccaagatgtt atctttgcaa ttcgtcttgt tgcattcaat tcttcggata
      841 tgcgatgtat aattcttttt atacttgaat gtttctgtgt catattcttt aacctctttg
      901 tattaattca aataactcat aatattttga ttctaagatt ttgtatgctg tttgaatatt
      961 ataatctgcc tttattaaaa tcgaacgttc tgaatctcga tgagagatat ctacatttgc
     1021 aatctctctc gcccattctt ttgccgattg atttaaactt atgaaattgg tcttatcact
     1081 gaaagcaact tctgtaggaa ctttctccga aaagaaacac ggtaaatctg caaattgagc
     1141 ttcaataccc actaacggaa ccccttcaaa taatgatggc aacattaata cgtcaaatgc
     1201 ttgataaaat ttttctatat catttctatt tcctaaaaat ttaatatcat catctatgcc
     1261 acttttcttt gcataatttt ttatttcttc ttccatatca ccagttccaa taattaataa
     1321 tacggaatta ttcttaatag cgtgaaattc tttaaaaatt tcaatcaaaa aacgatgatt
     1381 tttagaatat gaaattcttc caatatgtcc cattacaaac gtctgacttg aaattcctaa
     1441 tttcattcgt acatcatttc taatactttc atcataataa aattttttag catccactgc
     1501 atttggaatc aaagtgaatt tatcatttcg aaacaaaaaa tcacctgcta cttttccaca
     1561 tgaaaattca tgtgtcaaaa cagacgttaa cttacatcga taatattgct ttaaaagata
     1621 tttaaaatct ctatctatcg atgtattatg gctatgtgct atccgtatcg gaatcccagc
     1681 tttttttgca gcttttaaag gtaaataact catcgcatca atatgagaat gaacaatctc
     1741 atattctgga tgatccttaa aaaattgttc catccattta aaatatttag gataattttg
     1801 tggataaagt ctcggtgcat aatagacctt tcctccaaga cttattattt cctcatcata
     1861 atcactttta tatggacgat gtgtcaaaaa atcaaactga attttagttc gatctatatt
     1921 cctataatag ttcattagca tcgtctccaa ccctgcacga tgcatatcat tcacacactg
     1981 taatattcga atcattatat atttccccta tacaaattta atgtttcttg tacgatttta
     2041 tcccaattat atttactaca aataaactca gtagcttgat tcttcaaaac tttaaccatt
     2101 tccgattgat tgcatgcctc ttctaatctt atctttaaat cacttacatc cgatttctta
     2161 aaaatcatag ccttgtcttc tactacttcc gtacactctg agatatttga aactatacaa
     2221 cagttcccat aactcattgc ttctaacaaa cttaatggca ttccttctaa atcacttgga
     2281 agtgtataga tatacgcatt actatataat tcttctaatt cctgtccttg tacaaatcca
     2341 gtaaatataa ttcgctcatc accttttgct aattccttta gttcatttgc aaattcatct
     2401 gtatcagagc ttcctccggc tattataagt tttttatctg tctgaacatc tttaaatgcc
     2461 tcaattagat accgaattcc tttttcggga accaatcgcc ccaaaaacaa aatataagaa
     2521 tcttttgtta atccatactt ttctgtaatc aatcctgctg attttatttc aattttttta
     2581 acgccatttg gtataaacct tgtttttctt ccatatatat cttcaaaata attttggacg
     2641 ctttcactaa gtacaattat ttcatctgca aattttacag cacacttttc ccctaacatg
     2701 atgtatgtac tagcaagctt gttccatttt tctctttgat gatctagacc atgaattgtt
     2761 gcaacacatc ttttcccaaa aagttttggc aaccacaaca tagcacatgg tccttctgca
     2821 tgaaaatgaa caacatcata ttttccgaat gctgctctaa ttccaccaaa tactgaagaa
     2881 ctcattgctg caattccttt tatatttaat gtaaaaatag tctttaattt tacaccttta
     2941 tattctttat ttttatgctg gtcaaattcc tttccgctaa catgatgacc acttcgatta
     3001 tagcaagtca cttcatgccc aagtgcaacc attcgcgttg ccaattcttc taccactatc
     3061 tcaattccac cttctcttga tggaattctt ttatgaccta gcatacagat ttttaatttt
     3121 tttcttgcca tcacgtttta ctccttctat agtatcttta tcttacatgg aaccgtttct
     3181 atgaagcaca gttttgactg tcctaaacaa aattctaaaa tctaatccca tactccaatt
     3241 cctgatatat tctgcatcaa gttttacaac ctcttcgaag tctgtgatat cacttctacc
     3301 actcacctgc cacattccag taatcccagg tttcatcgca attctcgcac gatgatgaaa
     3361 atcatacttc tcatattctg acttcaagca aggtctaaac cccaccaggc tcatttttcc
     3421 cattaggaca ttataaaatt gtggaaactc atccaatgat gtagatcgaa taaattgtcc
     3481 aattcctgtc ttctttgttc catcaggaag tattttattg ccaatgattc ttgggtcaaa
     3541 atccatctta aacatgagat tattactcat cttattttct ttcatcaact ctgctttgcg
     3601 ttcttctgca tccatataca tactacggaa cttatacatc ttaaatgtct taccattttg
     3661 accaatacgt tcttgtgcaa agaaaatcgg ccctggtgat ttgatgtaaa taactggtgc
     3721 tacgaagata aaaataacac ctgttgcaat acatccaata attccagcaa gaatgtcaat
     3781 cgttctcttt ataaatgctt gcctcatcgt cataacatta atactagatg taataacagt
     3841 ataatcacca actcggccaa taaattgttt tccacgtgta ctatcagaca cttttgtaag
     3901 attcatatgt acagcaagtc ccatttccga acatctggcg attaattctt tgggatatgg
     3961 aacactttcg tcaacattga tgaatacttc atctacccaa ttctgtaaaa gatattccgc
     4021 tgcattatca gcatttgcaa ctactgggac acctccgatg gtagttccat acatatctct
     4081 gtctactaca atcacaccac taagtttata catgccataa ttcttctctt taagattacg
     4141 aactacattt tttgcaatct tttgagatgt tacaataact aacgatacat cttcaccgtt
     4201 tttcatgcgg tgatgcaaca accctttcca aagtatacga attatatatg ttaataatcc
     4261 atacaccacc cccattataa ataaagaaac tctggaataa tcatttccat cctgcattgt
     4321 aataaggtat aaactgcaca caagtaacag cataaaagat tgctttatag aagctacagc
     4381 ctctttataa tatcctcttt ttaaaacatt tttatatgcc tcaaataaaa atataatcgt
     4441 aagattctca agttctataa aaattgccat gtggcgataa ataggaatca catacggatt
     4501 ccagtcaccc tgtcgcatta catatgatat ataaaaagca agctggacac aaaataagtc
     4561 taataaaata aaatcaatat gtttagacca accacttgag ttttttctat acatctctca
     4621 ctctctcctc atttcctttt tatctacgaa taaagaacta aaataaacca aacttcttct
     4681 tttttacctc ttcatagatt ggataattga tctcatcccc gttgatcaga tcctttgcga
     4741 cttgtttcat ctgtactact ttttccttac caaactcctt ggcaatctgc tcatagcact
     4801 cttttaagta aaaggtgcgc ttagagaccc cgtgtgcatc agaagcagcc atctgcacga
     4861 gattatgttt gaccattgtt ttggctgttt tctggatatc tttgccaaat acaccaacat
     4921 aacttggcgc agtaagttgg gccaaacatc ccatatctag gaacggaagc aaatgatttg
     4981 ggtcttctcg aaaatgcgcg ttgcgttctg gatggacgat cactggtatc tttcctagtt
     5041 ctaacaactc aaaaaaaagc cgttctgcat ataacggcac ttccagtgtc ggaaattcga
     5101 tcagaagata tgtatcatct aaatctgtaa ataaaatttc atctcggtgg atgtcttcga
     5161 tgagcgttcc ggtaatacgt acttcttgtc cttccaataa cgtcaaaggc aactgtcttt
     5221 tctctaattc ggcttgtaac gaagcgacta gagaaatgac ttgtgatttt tcattgctgt
     5281 attttccatt attgtggtgt ggtgtgcata aaatatgcgt aatgccttgc tgaatcgctt
     5341 tttcagccat tgcaatactt gcttctaagt tttctgcccc gtcatcgatt cctggtagga
     5401 tatggcaatg tagatcgatc atttctcttg tctcctctct tcttattcct taatttccgt
     5461 agtaataacc agcatctttg ctgtgttctg ctccgttgta tacgacacct aagactcgtg
     5521 cttgtaccat atctaacagt tcttttgctt tagtcaatga ttcttttcgt gacgtatttt
     5581 cccgtacgac caataaggtg ccatccgttt ttgaagacat gatctgtgca tctgttacgg
     5641 caacgactgg cggcatgtcg aagatcacta catcaaatag ttgtcttgct tctgctaaaa
     5701 tctgatccat tcttggcgaa cttagtagtt ctgatggatt tggtggttta ggtccacttg
     5761 gcagaatcga caaattgtcg actggtgttc gttggatctc atctgctaca cttcctgatg
     5821 aactaagaac agtacttaat ccgcttgcgt tgttcaactt gaacgtttta tagatgacgg
     5881 gcttacgtaa gtctgcatcg actagcagca cccgttgacc agatttggca aaaacaacgg
     5941 caatgtttgc tgcagtggtc gatttccctt ctccaggtcc tgatgaagtc acaacaatcg
     6001 ttttgatttg ctgaccagct gcggaggcaa actggatatt cgtccgaatc gtacgatatt
     6061 gttcagcgat tggtgaagag ggattggtga gtgtgaccaa actgactgca tgagtttggg
     6121 ctgctttttg tttctgtgtt cgtgccattt tctttcctcc tagactcttg agcggcttct
     6181 gcgtgatggc gctgttttat cgtttgtagc ttttggttgt gtctgagctg gtcctttttt
     6241 acggatacca gcattcaatt ctttcgctga catttctgga actgttccta agattgggaa
     6301 tcctaatgta tctgtcacat atttttcatc tttcactgtc cgatcgagta attccaataa
     6361 gaatgccaga cctacaccta ccatcattcc taacattaag ccagtcatta aattcaattt
     6421 attatttggc gaaacaggtg tcgtattggc aactgcacct gaaatgatcg agattttatc
     6481 aacattcatc acatcttttg cgttctcttg gaaaacttgt gaagttgtgt tagcgatctg
     6541 ttgagcagcc actgcattcg tacttattgc ctgaatcgag aacatttgtg aattttctgc
     6601 ctgattaact gagatggcat ctttgatttg ttcagcagtc attttcacat tgtcttctgt
     6661 ttctaaacga tctttgactt cattgagcac taaatcactg acgatcatat ctttatacgt
     6721 attgatcatt tgcaggttcg tattgacatc attgacattt gcagtgtctg attgtggcag
     6781 tgtcacgatc aattgtgctt gtgaactgta ttttggtgtc aagatgaaaa atgttacgat
     6841 tcctgagatg gccaagccag caaacattga gatgatgatt attaccaagt gtttgcgtaa
     6901 tgtttcaaaa atttctttca agctgattgt ctcttccatt ttttctccac tttcctattt
     6961 attctctaga ttcatttgat cttttaattc ttttttcaca cgatcaagtt cttgatcact
     7021 gactttttga taggagatat tatcttgcat gaagccttct ccttgcagct gatcttgctt
     7081 aatcgtacca aatgcctccc gataatctaa agcaattttt ttcatttcat caaagttcat
     7141 gtctgttttc atattcgact ctagagcatt caaaacggat tgatagttgc tgacaccgtt
     7201 tagactcaat acttttttcg ctacgccttc tacgatcttt ctttggcgcc cttgtctacc
     7261 gtaatcacca tttgggtctt cataacgcat acgagaataa gataatgcct gatccccgtt
     7321 caatgtaatt ttaccgattg gaaagtcata gccatcttgt gagaaagtga ggtcattatt
     7381 gacttcgata ccaccaactg catcaacgag ttcctttagc cctttcatat tgatagagac
     7441 atagtgatcg attgggatgt ctacatattt ttgtacggta tccatcgcca tggcagctcc
     7501 gccgaatgcg taagcgtggt tgatcttgtc ttttgtgtca cgacctacga tgtcgacata
     7561 cgtatcacga ggaatgctga cgatcgttgt ctgtttgtct tcaggactga ctgtggcaac
     7621 catcatcgta tctgatcttc cttgatcgac ccgacctaaa tctccagtat caatccccat
     7681 caatagaaca gagaaagatt cttgtttgtc cagatcaact ggtttcgttc gttctccttg
     7741 ggttcgttcc actgattcgt aagttttctc aatagaatgt gagagatcca gatataattt
     7801 tgctcctact cctacaattg ctaatactaa tacgataaga actgctaaaa taatcagaaa
     7861 tatttttttc cctcttgatt ttcttcttcg tcttgccata gtttcctccg tttgctaacc
     7921 ttgcttgaat caacgtttta actgtcaata gcttatattg ctatagccca tttgctcagc
     7981 aacagtttgt gcttttgttg cgtatgctgc gcttattttt tcattccgga tctgctctat
     8041 ttgactgagc accgtcaaat attgatcgta agtagcggtg gcagtcactt gaccatcctt
     8101 taacatcgta tcgaataact gatctagttg cgtgacttgc gcgatctgaa tatcagcgaa
     8161 tcctagatat tgcataacaa ccgttttcca aggactatct ttaaaaaagc tcatattttc
     8221 acgtacacga gcgacatctg cacttgtcac cttttctttg atgatgacat cgtctacagc
     8281 ttgctgccag tttgatacat ttttagtaaa taaatcgttg acagctgtct ggatcttcag
     8341 ttttgattct gcactatcta actgtgtcac gattgctttc ttctcatctt ggattgcaac
     8401 agcttcttta ggcaattctg attctttgat gtcgtaatca tctgctgaaa gtttgacaga
     8461 ggatagctta tttgacaaac ttgtgattgt ttcttctgtt atttcagggt ttagaaaaat
     8521 tttttctttt gtactataca gcttgtctac ttcttttgat aaagaagtca aggtgtcttt
     8581 ttgattggtg atggcagatt ctgcataatt gatccgttgc tgatgttgcc gctgattatt
     8641 gatatggtat ccaaacaaac agatcgcaat agcacctgca cctaccgcca cagcagtcaa
     8701 tgacttgacg aatggctttt ccatagattc actcctaagt attgatgata gataaattaa
     8761 gatataattc acttacataa aaatgacagc tttttattta aaaaaacata attaaactaa
     8821 tatattcaca gaaatatttg t
//
LOCUS       DAZUKZ010000107         8495 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000107 DAZUKZ010000000
VERSION     DAZUKZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.109"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..150)
                     /locus_tag="ACG8EF_002310"
     CDS             complement(<1..150)
                     /locus_tag="ACG8EF_002310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4947594.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFLM"
     gene            complement(594..1715)
                     /locus_tag="ACG8EF_002311"
     CDS             complement(594..1715)
                     /locus_tag="ACG8EF_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFS4947595.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2043..3452
                     /gene="istA"
                     /locus_tag="ACG8EF_002312"
     CDS             2043..3452
                     /gene="istA"
                     /locus_tag="ACG8EF_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFS4947596.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3449..4177
                     /gene="istB"
                     /locus_tag="ACG8EF_002313"
     CDS             3449..4177
                     /gene="istB"
                     /locus_tag="ACG8EF_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFS4947597.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4305..5216)
                     /locus_tag="ACG8EF_002314"
     CDS             complement(4305..5216)
                     /locus_tag="ACG8EF_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4947598.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5233..6240)
                     /locus_tag="ACG8EF_002315"
     CDS             complement(5233..6240)
                     /locus_tag="ACG8EF_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS4947599.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6237..8366)
                     /locus_tag="ACG8EF_002316"
     CDS             complement(6237..8366)
                     /locus_tag="ACG8EF_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4947600.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8371..8481)
                     /locus_tag="ACG8EF_002317"
     CDS             complement(8371..8481)
                     /locus_tag="ACG8EF_002317"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947601.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1729 c         1486 g         2681 t
ORIGIN      
        1 cattaaaaag ttagtaaata attgccctct aagttccacg gtctgttcat taataacttt
       61 atatcctcgt ttttcaaaaa atggctttgc ggtaatggaa gcgtgggtag tgattttttg
      121 cataccagtt tctaaaacat ggttttccaa ttcatctgca attttagtcg caataccttt
      181 tccttgatgg ctatgttttt gtcacaactt cagtattcct gtaacaatac ggatttcagt
      241 atacaacaac agcttttgaa aagatacttg caaaaggatt aagatatcta tgacctgcta
      301 tgctcactga tgttgaaaag aatgttttat taggattgag gtaagcaaga tagtgaatat
      361 gtccggaaag cctgtaaaat aaggcttttc ggacatatgt gaagttataa aaagatagcg
      421 aaaaatctat ttagtatcac taatgttgcg aacatatcgt cagaatagtc tgacatttat
      481 ttcttgctaa aaatttcaaa aaagtcaagt gctgacgcag cggatgacta ttttccagaa
      541 aattttagta tgtattcaaa aatcaaacgt gacaacagct attaactaca tcctcatcca
      601 ggtctgtaac gtttgaacca ctcttctgcg ggctcaaaca ttaagtaatg gaaggtacgt
      661 ttcaattgaa acagcgtcgc tgtcgtattc aactcaattt ttatcacata agtgagaagc
      721 gttgcgatta aggtgaggat gacttgattt atcgcccctt gttcactatg tgaatatagt
      781 cgtttaatgg tcagattttg ttttaagtgt ttgaacaaca attcaatctg ccaacgggct
      841 tgatacatgt cggccacttc gtttggcgat acatcaaaga gattggtcac cattcgcagg
      901 attttttgtc ccttggtaag aaccgttacc aacctaaaac gctctgtcac atggttgaca
      961 ccgccgacaa gaatcacttg ttggtcactg attatcgtcc catcacgggt tgttgtatct
     1021 gctacttcaa tttgatccaa gatggtaatt tttgtattag attttgtccg tgtaacgaag
     1081 aaatagccgt cacgatttag tttgtctaac aacttgtaat caaagtagcc gcgatccacc
     1141 acatacgttg cttgcgtttt atcaacaaga acttcaaggt gattacggtc atgctcactc
     1201 gcatttgtca tcgtaaaact ttcaggatac tgattggttt tatcaagata acagagattc
     1261 aaatgtagct tgatgccaga ctttgttttc cgaaacttgg cccatggaaa ccatgctttg
     1321 ttcaacggaa tagtcgttga atcaatcaat tgtagactgg ttgtttttga gcttggctgc
     1381 tgagcagaaa ttctggcgac taattgcgta aaaatttcca tcaatacttc ttgcttgatt
     1441 tctggcgctc tacgagataa cgagctgtaa cacagggaat caatgccaag ttccttgcag
     1501 aggcgctgat ctaaaaaggc gcgatcaatc tcgcgatagc ttggcaattc ttcataaacc
     1561 gcatgaagta acactttaag cgtcgtttgg aagtctagtt ttttactgta gaaatcaaag
     1621 tcgctaatta agctttgaga gttttctgat aaaccagaaa aatttattgc agaaaaccat
     1681 ttttgaaatg aggtttttac gttatactta tccatgcgtg agtccttttt attggtcttg
     1741 gataagtcat ccgagaatag tataaaggac ttttttgcgt ttggatagct ttgtattagg
     1801 cttttgatgg gttaaaaagt ctttgcaaca ctagtaattt agtatttatg tataaattga
     1861 aagatatttt accaccactt agtcttctta aaagtgatcc aacattttgt ttgtggcaga
     1921 atttaactac acactaatgc agccgaaagt acacaactat gcagcagttt gactgcgaac
     1981 tggtttcctt ccatcataag tttgaatgac ttagtgatgg aggtacagaa aggttgttaa
     2041 ctttggatga tattaaaaca ataaaacatt tgagaaataa tcgtgatcag tcaattgatc
     2101 agataagaaa agcaatgaat atcaattggc gaacagcgaa aaaatacgcc gatagtgatc
     2161 aactattcat tacagatccc actcgaaaaa acaaatccat gatggatgat ggttggggag
     2221 aaatcatcgg tagttggcta gaagaagacc tacgtttacg cagaaaactt cgtagaacgg
     2281 ccaaagctat ccatgaacaa ttgaaacctt tgggtttccc tggctcctat cgaacagttt
     2341 gtgtatacat ttctcaatgg aaaaagcagc gttacgacga aacgaattac caagctgtcg
     2401 atcgtcttga tcatccgatc ggagaagcac aggtcgattt tggaacaatg gaagttatgc
     2461 atgaagatca catgaaagat atacaacttc ttatcatgtc atttccctat agtaatgcga
     2521 cgtccgcaag tgccatgcca gctcaaaacc aagagtgttt cctggaagca ttaaaacaac
     2581 tatttaatca atgcggcggc gttccaaaaa cgatcagaat agataactta actccagcgg
     2641 tgaaaaaaac aaaatcaaaa tttgaagaag cacaactaac cgatgaattc ttacgtttca
     2701 ccattcatta tggttgtgat gttcaagtct gtaacccacg tagtgggcat gaaaaaggac
     2761 atgtcgaaaa taaagtgggt tatgtccgat acaatttctt ctcgacttcg ccaatcatgg
     2821 actcttttga gtccctaaat aagcaacttg aagaacaatt aaaagcagat cgaaaaagga
     2881 cgcattattc aaaacatcaa ttaatcgaag atttgtggaa gaaagaaaat aaagcgttac
     2941 attctcttcc acaagaggat tacccaattt ttaaagaagt ggaagctaaa ctaaatcgtt
     3001 atcacgaaat gaaattggat caaacggtta ttcatattcc taaagcatat ggctattctc
     3061 aattgactgt tgttttgact tggaataaat tcaaagttct tacgcctcat ggagaaatat
     3121 taatgaacga atatcgcccc tatatgaaca aaaaaagatt gatcccatgg agtgacatca
     3181 tgaaacaatg gagtaataaa ttgaatgtcg ttagttattc gaggtattgg aagtacttgc
     3241 ctgcacgcct tcaaaattat ctagcgattg ataataatca cctgaaatac agacggataa
     3301 aacaattact aagtttatta gcgacctacg atatccaaca aattaatgaa caattttacg
     3361 aattagtcgt tcaagaagac gccccctctg actttgatgt gaattggtca aactatgatg
     3421 cattaacaaa aacaggaggt gtgcaaaaat gattggacag acgttagaag attattgtaa
     3481 aaagcttcga ttaagccata tcatcacgaa cataccaaga gcttctttcg aagctccaga
     3541 acaatttatt accgatttat tccgtttaga actggagacc agacagcaga tcaaattaga
     3601 acggatggaa aaattagcca aatttcctgt accaaaatcg ctaaagaact atgaatggca
     3661 cgaagatatt tttctaccat ctcatctaga taaggagacc cttgtctctc ttgattttct
     3721 aaaaaggcaa gaaaacattg tttgtgttgg ctctcctggt acagggaaaa cacatctagc
     3781 gatcgcatta gggagaaaag cctgtagtga aggtgtttca accaaatttt tccgagttaa
     3841 caatctcgta gagcaattag agaatgcatt aaaaaaacaa cagctagcca gtttcaagaa
     3901 gcgattcgaa acaacagaat taattattct tgatgaaatg gggtacttac cattgacaaa
     3961 agaaggagct gaacttctct tccaactgat ttcagattgg tatgagcaaa agagcattat
     4021 tattacctct aatttagaat tcagtcaatg gaatcgagtg tttatggatt ctcgattaac
     4081 agctgctctc gttgatcggc taatccatca cgctcacatt atcagcttca aaggcgaaag
     4141 tttcagattg aaaaattcat tatctaaatc atattgaagg aaaaaactag ttgcagcaaa
     4201 actctgcact ttcggctgca tttttttgta aaaagctatt gcacaacaca aacatttgaa
     4261 cgacgtaatt ccaaatgttg gattgctttt agataatctt ttatttattc acaattgcta
     4321 atagtccaat tttcattttt gtatagtttt aatctatact gaaaaacact tgttgttttg
     4381 gacaactcat caagatactc aacaccaaca atcacttgaa tcgctttacc cttttgtttg
     4441 taaataggat tcactaattc cacaaatttc aaattagtat taattggttg catcgcgtct
     4501 ttttcaacat aatgctctag ttctttttct gaggctgctg gataaagcct gaaaaacgtc
     4561 tctaaaaact cggtgatttc ttccgttgtc tttgaatcaa tttttgaatc actttttact
     4621 tgtcttactt tatgttttgc cttttctggc ttaccaatca tagtgggtaa actcgttaca
     4681 atatgattgc ctttttcatc ttcaaagatc atcagacgat atgcagaaga aattgattgt
     4741 gtctttttcc cttctgtaat cttttgatct accacaaatg aaacgacaaa ttcttccgag
     4801 tgtttttcaa catctagaat ttttactgat acaacctcgg aactagtggg aatatccgct
     4861 cgaaccatat cttggctcaa agcgagccct tcttctgtaa ggtactgctc aagattagtc
     4921 attctctttt caattacctc cttgttattc ttccatgaaa aatattcttt cacaaaatct
     4981 acagtaaaat tttctactcc agaggtattc actattttct ctttcacgac cactttctca
     5041 tgagttgtat gctgatcaat ggccgtgaag tttttgtaga ttccaaaagc aaggctacaa
     5101 gacaataaaa gccaaagggc aagcaccatt tttcgatgct tgcccacgga tactactcgt
     5161 tctttcttct tttttggaat cttttccttg ttacttcgct caatttttat cttcattttc
     5221 cattcctctt ttctattgtt tataccgacc agcaccaata atgtgctgtt gccaatagct
     5281 atcggtaaga ttggtccaac ccaccggatc tcctgcatga aacattctgt tctctcctgc
     5341 ataaatgccc acatgagtca cataagtacc ggcatcataa gttgaatgaa agaaaatcaa
     5401 atcgcctggt ttggcatctt ttaagtcaat atgagtcata gcatcatatt gatcctgagc
     5461 cacccgaggg agtttcaaac caactttggc ataacaccat tgagttaaac cactacaatc
     5521 aaacccagtt ccgggacttg acccaccaaa tacataagcc gttccttcat acttgaacgc
     5581 ctcgtcaaaa attccttgga ctgttttatc atcaaactgt gttgtagtta ggtactgctt
     5641 cactaataag caataaaaca tattgccata gttataccgc cacccactat tttccttaat
     5701 cgcgatggga ttgggataat cgactttttg accacccgat ttttcttttg aaaaagattc
     5761 ggctaaagca aaactatgct tctttccgtt tttcgcgaca taatccaaat acccgtcacc
     5821 atagttataa gcctgaatga tactatcttg atcgcatcct agttctttgg aacacttaac
     5881 caaagacgaa aaatagagac acacttgttt gatggatgcc tcggtactta atgaattcgg
     5941 tgataaacct gctgattcag aactttgcat gacatcttcc attgtacctc cagattcgac
     6001 ttgaataatc gccaaaagaa tcgaaacata ctctgttata tctgattctt tggcatactt
     6061 ttccaccatt gaacggtgtt tcaaaactac ttctgagaca ctactacctc caggagaagt
     6121 tccagaacta ccacttccct caccgtcctc accaaacaac aaacccacac acatcaagat
     6181 gcccacaaaa gagatggtca ggaaggttaa cgaaataata ctcaattttt ttaagttcat
     6241 cttttctcac ctggctttgg ttttgggcga gtgttctttt ttctttgtac ttgttgattg
     6301 gttcgctttg gaaaagtgaa tctctgtttc acttgttgaa aggaattgtt cttgcttagt
     6361 ggctgagtgc ttccgtttgg aatgacttct tgactagaaa gtttccgttt gatttcagga
     6421 ttcgaatgta cttgtaccct tggttttaaa gacctatttt ctggtagttc atttttctgt
     6481 ggttttatct ccttttttat cggaacattt tcccttcctt tctcatgacg attccatgct
     6541 tcgttcaata ctttgcgttt ttcatccatt tttgcgtctc ttcgttgtcg ttgtttttct
     6601 cgcaactccc ctcgatttgc tttttcttcg aaaggccctc gcttaaagtc ctctggtact
     6661 tttttcgtct tttccatgcc tttatgaagg ctgtatttca agttagttgg tgcatcggtt
     6721 acttgatttt ttgcttgttt cgctttgtcc ttcacgattt cttttgtatc gagtactttt
     6781 ccaacttttc taccaactaa atttattcgt gattctttct tcgtgcccac tggttgttct
     6841 tgttttttca gatcagaatt tagctcgtta atgcttctcg cggaatggtt ctttctattg
     6901 gacactctat tgatttttga actacctttc ccttttgtag aactggtagc tagtttccca
     6961 ccaactgctg caccgccacc aaaagcaaaa gttcgaccca catttcgatt aagctgtcgc
     7021 aattttcgat tcatcaacat ttgcggtttc cgcatgactc gccttcccat tgactgacta
     7081 tcattacttt gaaggttaaa catgctcata atgtcaccta atttgaaata gataccggca
     7141 aaggtcacaa tttgtagaaa agctaccata aagaagggat aggtggccga aatattgaaa
     7201 aacatcgtgg agatactgaa tgcagtcgta atgactaagg taactcccgc tcgcatcata
     7261 atcgtattga acaaacgaat aatcgccctt ttgcccaaac tctcatacgt cggcaacatc
     7321 gacaataaga aactaatggg cagaaacatc gcaaagataa tgaaaaggat ttgtgagaac
     7381 aacataatcc ctgtcagaag aaacacaaaa aaggaaatcc caatattaaa caacactaaa
     7441 aagaccacgg ttcctaaacg gttcatggtc ttggtaatac tgagattcgc attgtcatta
     7501 tcttcaatct ctgctttgac tgcttcttcc cgatccttac ccttattttc atccggactc
     7561 acggataaaa tcttattaac ccgaccctcg ccaatttctt ctatattgga atcatcgaat
     7621 tgtaatagta accagggttg ttgaacttga atcgaaaata aactgtctcg aatcaaatcc
     7681 acactatctt ttccctggct ttctgaatta ggtacaacaa tcttggttcc aaggctaaga
     7741 gccgcttcac taacgtctga gctaaagtca ttgatttttg taatataggt gggcgcatac
     7801 gcaatgaaag aacccgagag tagaaaaatc atcacaaaat taatgacggt tcggactgct
     7861 ttggtagttt ctcgttttaa taatccggta tacgccacat aaactcctag agctaaaatc
     7921 atcaataata ggaacccaaa gtagaatccc gaagtctgta atccattttc agtgataccg
     7981 gctaaagttt ggatattctt tccaatactt ttagccgtat ccgaaatgaa atccaacttg
     8041 taagcttctt gaaccacata gcccgtggcg tttgataaat acagacttac tgtccaaata
     8101 aaattggtaa tggcataaag accatacatc acgcttttcc ctaagccatc cccccaattc
     8161 caaggtaacc aatcccaaga actatccaca aagaaatcca gttgataatt gttcagagaa
     8221 tatttggaat aaaggtttcc ggcttcaact gtatcatcaa ccagacctgt cgcctcagct
     8281 accgtaccga ctaaactcaa tagaactgtc agagtgacga tgctaataaa aacgattccc
     8341 acaatccgaa aaagtttctt tttcatcccc tcactccatt ctctcttgaa ccggcggacg
     8401 agtgtcaaaa gcatggaaga gttcttcaaa gactggatga acttgaacca cacctactcg
     8461 cccatacaag tcttgcatca agcattgacc gtttt
//
LOCUS       DAZUKZ010000108         8386 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000108 DAZUKZ010000000
VERSION     DAZUKZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.110"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..480)
                     /locus_tag="ACG8EF_002318"
     CDS             complement(<1..480)
                     /locus_tag="ACG8EF_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFS4947602.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     "
     gene            complement(610..1320)
                     /gene="budA"
                     /locus_tag="ACG8EF_002319"
     CDS             complement(610..1320)
                     /gene="budA"
                     /locus_tag="ACG8EF_002319"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFS4947603.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1333..2997)
                     /gene="alsS"
                     /locus_tag="ACG8EF_002320"
     CDS             complement(1333..2997)
                     /gene="alsS"
                     /locus_tag="ACG8EF_002320"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFS4947604.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3205..3867)
                     /locus_tag="ACG8EF_002321"
     CDS             complement(3205..3867)
                     /locus_tag="ACG8EF_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4947605.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3877..4692)
                     /locus_tag="ACG8EF_002322"
     CDS             complement(3877..4692)
                     /locus_tag="ACG8EF_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4947606.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4953..5396)
                     /locus_tag="ACG8EF_002323"
     CDS             complement(4953..5396)
                     /locus_tag="ACG8EF_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFS4947607.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5530..5868)
                     /locus_tag="ACG8EF_002324"
                     /pseudo
     CDS             complement(5530..5868)
                     /locus_tag="ACG8EF_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5856..6233)
                     /locus_tag="ACG8EF_002325"
     CDS             complement(5856..6233)
                     /locus_tag="ACG8EF_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4947608.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6457..7650
                     /locus_tag="ACG8EF_002326"
     CDS             6457..7650
                     /locus_tag="ACG8EF_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4947609.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7816..8238)
                     /locus_tag="ACG8EF_002327"
     CDS             complement(7816..8238)
                     /locus_tag="ACG8EF_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4947610.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2465 a         1692 c         1416 g         2813 t
ORIGIN      
        1 aaaagcattt atattaaaat ttgctaataa aagatctaca tatttttcta gaaatgcttg
       61 atcgatttca tcataccctt tcgttactcc gcaatcgata tctaaaacac cgattaaatt
      121 accttctttt accattggca ctacgatttc tgattgtgcg gctgaatcgc aggaaatgta
      181 atttgcatgg gttttgacat catcaacgat caatgtttct tgtttttcag ctgattctcc
      241 acaaactcct tttcccatct tgatacgtgt gcaagaaact cgcccttgga acggtcctaa
      301 aatcaattca ttaccatcga ataaataata tcctgcaaag acagtctcag gcaaagtctc
      361 attcaacaat gcagatgaat ttgctaaatt tgctaaccag ttatcttctg cttctaataa
      421 tccttgttgc tgtaataata aaatttcgta tgcatctttt ttctgttttt ctgtccacat
      481 gataaaaacc gctccttttt gatctatgga gaagattata gctcagtttt tttgtaaacg
      541 ctaggtttcc tcctcaaaaa aacgagatac gtcaggttaa tcaccttgaa cgcatctcgc
      601 tgtctttttc tattctgctt gttggatttc tgctgctaaa tcagcataat caatcgtttc
      661 gtgaacaaaa gtcggatcat caactggaaa atgctgtaca agggacgaga tgcggctaat
      721 ctcaactttt ccttcagcca cttcaaaatc cattacatgt ccgccaaatg tatgtgtatc
      781 atcgataaaa tgcaggtgaa atcccgctgc agcaactcct tggaataatt caggcgtaaa
      841 gaacccgact aatgttcctt ggatattgct ttgttggaat tctggttgag actctgaaat
      901 ttttgctagt cttctgtaag gtttttcttg cttcggcatg atacggatgt gcatattttt
      961 gaataatcca gttatcttca ctgccaaaaa taggttttcc ccggcttctc ttactaatat
     1021 gtcttttttc aattgttctc catctgcagt ttttgagaca gaaaaactag tatctggcgt
     1081 aaaatctgta attgctgcat agggggtaag ttcacttcca tctaattggg caactgagcc
     1141 atctgatctt ccttgataag ctacgccatc taaaaagatg acttcaccat caagcccatg
     1201 caatgttccc aatcccagag ttcctttttc taatatatga gcaatcgttt ctgttccatc
     1261 catcaagccg gccatcaaag ctcctaatgt cccgtgctgg tataagattt tttcagtcat
     1321 gtcgacactt ccttaataga attgatctgg taagattgtt tctcccaatt tctcattatc
     1381 acggtaatca atcggaatat caataattac gggtccttct gtcgcaaatc cttcttctaa
     1441 agctttttct aattcctcta cacttgtagc tcggattcct ttagcgccaa aagcttctgc
     1501 atatttcaca aaatcaactg gaccaaagtc tacacctgac gcccgctgat atttcatttt
     1561 ttcttggaac tctaccatat tgtagtgtcc atcattccaa atcaaatgaa cgatattcaa
     1621 ttttttacgt accgccgttt ctaaatcctg ggcagagaat aaaaatcccc catcgccgga
     1681 aactgatacg atttgtgtat ttggacgcac caatgcagca gaaatcgccc atggcaatgc
     1741 aacaccaagc gtttgcatac cattactgaa taacaaatga cgaggttcat aacttctaaa
     1801 gtggcgtgcc atccaaatat aatgactacc tacatctacg gtaacagtca tatcatctgt
     1861 tacttttgac tgaagcgtgt taataacttc taaaggatgc aagatacctg cttctccttt
     1921 tgattgaaca atgtcccgct ccgttaattt tgcttgaaga gatgctaaat attcttttgc
     1981 atcttctgac acttgttgag gctctaatga acctgtcaat aaatcaagag tggctgagat
     2041 atcaccgatc aattctcttt caggctgcat aaaaggatcg atttcagctg gcgcttcatc
     2101 gatcacaatg atccgcgcat ctttttccgc attccagttt cttgcttcat attcgattgg
     2161 atcatagcca attgcaataa ctagatcact tcgtttcaaa agcatatctc ctggttggtt
     2221 tctaaataaa cccacacgac caaagaaatg cgcttctaat tcacgtgaga tcactccagc
     2281 tgcttggaat gtctctacaa caggcaatgc tgtcttttca actaattgcc tgatggctaa
     2341 cgtttctttt tcacttgatc ctcgcatacc tactaatagt accggtagtt ttgcttctct
     2401 aatgcgttca gctaggtaac ggatatcatc tgcagaagca gaacctaatt taggatcgct
     2461 caatggcttg atgacattac cttggacagg tgcatccact acatcttgcg ggatactgat
     2521 aaagctcgcg cctttttttg aactcttagc catacgataa gcattggcaa tcacttctga
     2581 aattgtttct ggatcttgga tttctgcact gtattttgtg attggctgga acagtgctgc
     2641 attatccatg ctttgatggg tcaacttcaa tagatcgctt cgttttactt gtccggcaat
     2701 tgctaaaact gggtcacctt ctgctgtcgc tgttaccaaa cctgtagcta agttactagc
     2761 accaggacca cttgttgcga taactactcc tggctctcct gtaatacgtc cgattgcttg
     2821 tgccataaat gcagcatttt gttcgtgacg agtcacgatt aattcaggac cttgatcctc
     2881 taattcatta aatacaccat cgatttttgc acctggtata ccgaatacat aatcaacatg
     2941 atgattaatc aaactttcta ctacagattt cgaaccttgt ttcatttttt cagccattga
     3001 acatcgtcct ttttatgctt tatttttaat tattagttag aaataatcac aaataagtga
     3061 tacagtttct aaacctgttt catacaaaag tatagcacgt tttatttcat ttgtttagcc
     3121 atatgtttca aaaaatgaaa aagaatgaca gtttttacat aaaaaaaact gtcattctta
     3181 attgaaagtt tcaatttata aagtctacag atccatagtt ctcatcaatt tacgattgtt
     3241 gaagtaaatc aaaccaaata cgatagaagg acctagtaga atacaccatt ggggctttac
     3301 gaaaaagaag agaaacaaac ttgtcaatag taaaaaatct agtaaactca cataccattt
     3361 tttcaacgta tagtagaacg ctatccgtag aacttgattt ggtttcgacg ctggatttct
     3421 cacctgaaaa taccagcagt tggtgctaaa ggaaactgat aaacaagcaa gtaaaacaaa
     3481 caacggaatc aaccattttc cataagaaaa acgtataaaa aagaaacaat caaaaatcat
     3541 tatcacacta actatccaag caaatagcca ataaaaaaat ccctttagcc aaaattggcg
     3601 aaacatcttt atgaacaaac gtactggtga aaaactattt tcttttgcta attcttctgt
     3661 catacctatc atcacgatgc aacctggacc aataaaaagt aaagaaaaaa taaaggaaaa
     3721 gacattcctt ccatctatcg acaaaaggaa cgcagctgct atatacaaga tattgacaca
     3781 ccaaaaacaa aggctacttg ccaatgattg atacaaccaa taaaagaatt ccatataaac
     3841 atttttgtca aaacgctgct ctttcataat cacctcctat tccttcattc ctgatgaagc
     3901 gatcccttct acaaaatatt tttgcatcgc taaaaatata aaagcaacag gaagaacaga
     3961 aacaactgag gcagccatga ttaatgcgta ctctgcatca aatagcccaa taaacatttt
     4021 caaccctaac tggattgttt tattttccgt agaagacaaa taaatgaatg gccccatata
     4081 atcgttccat gtattaacga atgtgatgat cgtcaaactg gcaatcgccg gttttgtaag
     4141 aggtaaaatg atcttgcgat agattcccca ctctgataag ccatcgatcc gcgcactctc
     4201 acataaagaa tctgggatac tactgtaata ttgcttcaac aagaacacac caaaagcatt
     4261 gaacgcttgt aatagcacca acgaccacaa cgtatctgtt aaacccactt ttctcatcat
     4321 aatgaactgc gggatcatat aagactgcca agggacagca atcgtgccga tatatgctaa
     4381 aaataaaata tcgcgccctt taaaatttag tttagaaaac ccgtaagcag caaaactaga
     4441 agtaaacaac tgaatcaacg tgatcacaac gcttaaaaaa gctgtattct taaaaaatgt
     4501 cactaaagga atacgagacc aaataaccag ataattctct aaatgccaag ttttagggat
     4561 ccattgaatc ggaatcgtga acacttcatt atttgctttg aatgaagcag aaaccatcca
     4621 taaaaaagga atgatcgtca ataaagctaa tacaatcagc agaatcatac ccaaccattg
     4681 aataaagggc atgttttttg aacgaatttt tgcttttttt ctttcatttc gaacacctcc
     4741 aaagttataa tcttcatttc tttgtaatga ttacaatcca gaacaacggt tattatagct
     4801 gattcatttc cgttcttctt atttttgtaa gcgcttattt aacaagttat gtaaatctct
     4861 ttattttttt gatttaaaaa tgaaaaaata caaaagcaag actgtcatac gtctcgcttt
     4921 tgtattgaaa tttcttcgat tagagatact tattactgtt caaaagcttc tttgaaacgg
     4981 tgaatcaatt cattgactac aggtgataaa atccgattct ttttccagac tagcatgcag
     5041 ttcgtttcaa tcattgggtc aagcggaacg aatgtcactc gctttgaatg atgacttaca
     5101 gcaccttcta tgactaaagc agcacctacc tgattctcaa ttagcgcaaa tacattgaaa
     5161 atcaaattgt acgttcctgt gatcagcaac tgctcctttt ttatgcctaa ccagtcagca
     5221 aacattcgct gtatttctgg acgctttgag atcaaaagag gtacattcgc aaagtcttct
     5281 tttgttatca cgtctttttt tgctaaaaca gaattttcta aaatgagcaa tccccatttt
     5341 tcctttcttg gtaacaaaat cgtttcgtat ttctctgtat ctactggatc tagcaaaatc
     5401 gctaaatcta acaggctttt ttctagtcac tccgtgatat catcacttgt tccgctgtgg
     5461 atatgaaatt gaacgctggg ataatcacgg atcaactttt ctagtaatag tgccaatgta
     5521 ttcgaactat cagcttccac acagccgatc gtaaatcggc cgctgaataa ttgttttttt
     5581 ctgttctcga attcttgagt ggtttgattc gttaaatcta aaatttcttc tgctcggctt
     5641 ttttagaaaa taacctgctt ctgtcaagat cattttgcgg ttttcgcgta caaacagttc
     5701 tgttcctagt tactcttcta actccttcag ttgtcggctt aaggttggtt gtgtaagatg
     5761 tagaacttct gcggcttgag agattgttcc ttcttctgca attgtccaaa aatattttag
     5821 taagcgtaac tccatagcga tgctccttga tttttttact tgatacattc cattttattt
     5881 ctagataaca cacgatcata tttcttgatt tttccttgta aacgttccaa acattcttgc
     5941 atttcagcaa tttttttcgt caaaagttca ctttcttcaa tcaaaagctt acgccgctca
     6001 aatacagttg cttctccttc gctagatagg cgaatatatt caatcaaaga ctcgatcgat
     6061 aatccggcat ttcgcataca gatcgtaaag tcgacccatt ttaggtcttc ttctgtataa
     6121 tcacggattc cgccttttgt ccgattaact ggtggaatca acccgattcg ttcataatag
     6181 cgcaatgtat cggccgaaat gcccttttct tcactgactt gtttgatatt catctgatca
     6241 ctctttctct taatttcttg ccactttact tatgtatttt atttattcta caactttaag
     6301 tgtactccaa gtcaatactc atttctatta aaactaagct ggattatcta tatcggttgt
     6361 ttgtgtatta tttcaaataa aaaagtcttg tttttttatt cttttttgga tatactttta
     6421 acaatttagg gaaattttag gaaaaggggt ataaaaatga caacgtatca gcgaaaggta
     6481 ttagtatcga catcggcggg aattgcttta gagaatatgg atattatgtt tttagctttt
     6541 tcattatctt caatgattac ttcttttcat attagtggta cgcaagcggg tttcatttca
     6601 acgatcacta atttggggat gctcattgga ggtatttttt ttggcatcat agctgataaa
     6661 tatggacgtg tgaaagtatt ttctcaaacg gtccttctgt tttcgattgc ttctttattg
     6721 atgtattttg cttcaaacat ctacttggtt tatctctttc gctttatcgc aggtgtagga
     6781 gcgggaggag aatatggggc gtgtatgtcc ttgatttccg aaagtttttc aaaaaaacaa
     6841 attggacgag cttcttcggt tgctggtatt ggtgcgcagg tcggtgcggc tttggctgcg
     6901 attttagctg ctgtgatcat tccaacgttt ggttggaaga tgttgtatgt cgtaggtgta
     6961 ctgccggtgt tgatggtctt gttcattcgt cgcggcttga ctgaaccaaa ggaattcaaa
     7021 tctgtacaat ctgccgaaaa gaaaaccaaa cttagcagac tgtttgaatc aaaacatctt
     7081 gcttggcaaa catgtggcct tagtatcatg gtaactgtcc aaattgccgg ctattttggg
     7141 ttaatgaatt ggctaccttc aattatgcaa aatcgattag ggctttcggt ttctgggtct
     7201 tcattgtgga tggtcagtac tattttaggc atgtctttgg ggatgatgac ttttggcata
     7261 atcatggata aattggggcc taaactagcc ttttctttgt ttttaatttg ttcatcctta
     7321 tctgtctatc tacttgtgtt agcccatagc cagtggagtt tggtagcagc cgctgtgata
     7381 gtcggctact ttatcaatgg catgtatggt ggatatggcg cagtgattag tagtttgtat
     7441 ccaacagaaa tccgcgcgac ggcgaataat ttcgttatga accttggacg agctgttggt
     7501 ggtttttctt ctatcgtcat cggtttcttg atggatcatt atagtttaac tgccgtaatc
     7561 ttgtttttga gcggtattta cctagtcagt ctgctggtct tgacaactct tactggtgtt
     7621 acagcattaa gggaaagctt gcagcattga tagttgagtc tgtaaatgac aagaaagagg
     7681 tgtggaaaaa gcttgcatct gcggtaataa gaccagccaa atcaaaaaca tgagacactg
     7741 tttccgtttg gctggtcgta ttacttgatg cagggcagct ttttcaactg ctaatttcct
     7801 tctatctggc ggcgactatt ctttgacgtc cgtcatttca aagtacttta ctaataactt
     7861 agcataacga aaaactgtgg atacactcgt atattgagcg tctgcataat cttccaaatt
     7921 tttctcagga tgttgataca tgaattcaat caaatgataa ttatatgatt ttctgtagaa
     7981 atgaagataa ataatgtaga ttgggaagtt ctcctgcttt tccatagaaa agcgccgatt
     8041 attctcattc ttcaaaatga ccttatcagg atattgttgg aacaattctt ctaaatctgt
     8101 tagataattc aagatagact tacttgatac cttcaattcg ttttgtagtt cttctattga
     8161 acaccaacgt tctgtttcaa gcagtaaatg aatcatttta attttctttt gagaaacttt
     8221 tgttaaaaaa gtctgcatat tttctcctcc tgtatctact ttactatact acatacaaga
     8281 aaaaaacgga tacaaatatc atattgtcac accgtcaata gtgcataaac ctgttattat
     8341 ttctcttttt ttcctaagta ttctattgat atgaatcatt taatcg
//
LOCUS       DAZUKZ010000109         8209 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000109 DAZUKZ010000000
VERSION     DAZUKZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.111"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG8EF_002328"
     CDS             476..1339
                     /locus_tag="ACG8EF_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4947611.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG8EF_002329"
     CDS             complement(1468..3090)
                     /locus_tag="ACG8EF_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4947612.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG8EF_002330"
     CDS             3339..3860
                     /locus_tag="ACG8EF_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS4947613.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8EF_002331"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8EF_002331"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFS4947614.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG8EF_002332"
     CDS             5941..6759
                     /locus_tag="ACG8EF_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFS4947615.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG8EF_002333"
     CDS             complement(6862..7059)
                     /locus_tag="ACG8EF_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947616.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG8EF_002334"
     CDS             7228..7806
                     /locus_tag="ACG8EF_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS4947617.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG8EF_002335"
     CDS             7901..8038
                     /locus_tag="ACG8EF_002335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947618.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2460 a         1480 c         1619 g         2650 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaata
//
LOCUS       DAZUKZ010000110         8154 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000110 DAZUKZ010000000
VERSION     DAZUKZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.112"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..1248
                     /locus_tag="ACG8EF_002336"
     CDS             94..1248
                     /locus_tag="ACG8EF_002336"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFS4947619.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="ACG8EF_002337"
     CDS             complement(1270..2022)
                     /locus_tag="ACG8EF_002337"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4947620.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="ACG8EF_002338"
     CDS             complement(2386..5052)
                     /locus_tag="ACG8EF_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4947621.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="ACG8EF_002339"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="ACG8EF_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS4947622.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="ACG8EF_002340"
     CDS             complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="ACG8EF_002340"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFS4947623.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2369 a         1694 c         1455 g         2636 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacca
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa
     7081 taagtccaac caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg
     7141 atgcagggca actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga
     7201 ttgatggggc catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag
     7261 tcttttgcca atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata
     7321 catgcagata gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt
     7381 aaccaaaatg attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca
     7441 tttttagcat gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt
     7501 gctgcttgca caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca
     7561 taaaaattag gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa
     7621 gcagattctt ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat
     7681 ggtccaaaat catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct
     7741 agcatacctg ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg
     7801 tcgataaaat ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca
     7861 catgcggtca tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct
     7921 tgcatccctg cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga
     7981 tcttccatca aatcgattcc tccattttta taaaaagatt tttcataata ctgttactgt
     8041 ttctatcata tgtttttcaa agtatccctc agtaaatata ccactacctt ttcttggtaa
     8101 tcaaatttat ttgaaaaaac atttgcaata aatcagaaaa tagtggtcgt ttat
//
LOCUS       DAZUKZ010000111         8024 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000111 DAZUKZ010000000
VERSION     DAZUKZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.114"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..840)
                     /locus_tag="ACG8EF_002341"
     CDS             complement(<1..840)
                     /locus_tag="ACG8EF_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4947624.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMD"
     gene            complement(1064..3004)
                     /gene="thrS"
                     /locus_tag="ACG8EF_002342"
     CDS             complement(1064..3004)
                     /gene="thrS"
                     /locus_tag="ACG8EF_002342"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFS4947625.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3341..3520)
                     /locus_tag="ACG8EF_002343"
     CDS             complement(3341..3520)
                     /locus_tag="ACG8EF_002343"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFS4947626.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3567..5390)
                     /locus_tag="ACG8EF_002344"
     CDS             complement(3567..5390)
                     /locus_tag="ACG8EF_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4947627.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5668..7947
                     /gene="helD"
                     /locus_tag="ACG8EF_002345"
     CDS             5668..7947
                     /gene="helD"
                     /locus_tag="ACG8EF_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFS4947628.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2352 a         1708 c         1452 g         2512 t
ORIGIN      
        1 atccataaaa tattcttgga ttgcttctcg tgtttttttc actttttcgc taagcttttc
       61 gtttttagcc atatagaaat aaaaaatgat atcgatcgta gcaaattggg ccaaaagtga
      121 attagttgcc gcacttcgat tcgtgatttc ttttggtcta gatgtttgta cgagtacatc
      181 ggctatcttg ctaagcgggt tatttccaaa ttgcgtcaac gcaatgattt cgatattcat
      241 gcttttcgcc agctcggcta atgcgactac atctgcactt ctacctgagt ttgaaacaag
      301 ccaaagcact ttctttttct cattgctgac aagttgcggg agcaagacgt ggatatcttt
      361 ttcaaaaatg attggcttac cgacacgaga ccatttttgc agaatatctt ctactactaa
      421 agaagaggcg ccaaccccat ataaaaaaac agtctctgcc gattccaaac actctacgac
      481 tttttggatc gtttcttctt ctagaatatc tactgtttct tttatcgcta cttgtgcatt
      541 gttaccaagt ttgtttttga tagagtaaaa ggactcattt gcttctatat cactaaaacc
      601 ttcatcgatt ccagtctgta gttctgttga aatcgtgatt ttgaagtccg tcagactttg
      661 aaaaccgatt tttttggaaa aacgtacaac tgtcggcgca ctcaacccgg aagcttctgc
      721 taattcatta gctgtcattg tagggacctt ctcaatatta ccgataatgt agttggccaa
      781 ctttttttct gctgaagaat aatcttccat catactttca atacgactaa ttacgctcat
      841 aagttcctcc acccttttaa tgatagtcta tgtaagtata ccatatcaga cccttattca
      901 cggtcgtata ctggataaaa aacaaaaaaa caatccgccc aacagaatat cttatcccat
      961 caatcccaca gattctattg agcatctgtg tctttgagga atacctgtta tctattgttc
     1021 ggattgcttt ctatctacta cttttttaca ggaaatttac ctattagttt tctctgctgt
     1081 aatttttgac ttctgcttcc atagcatcaa taaagatatt caaatcttct acagctgttt
     1141 ctttacttcc ataacgacgt acgttcactg ttgcatcttc caattccttg tctccaacaa
     1201 ctaattggta agggatcttt tgtgtttgag aggcacgtat cttatagccc attttttcat
     1261 tacgatcatc aacttctaca cgcaacccct tcatttgcaa acgttctttg atttcataag
     1321 cataatcgcc atgtgcatca acagaaacag ggatgattgt tgcttgtata ggtgccaacc
     1381 atgttggaaa agcgcctttg tatacttctg ttaaataagc gacaaaacgt tccattgtag
     1441 aaacaattcc gcggtggata actactggac gatgcgtatt ttccccgtct tctccaacat
     1501 atgtcaaatc aaagcgctct ggcaataaga agtctaattg tatagttgat aatgtttctt
     1561 ctgtaccaag agctgttttg acttgaacat ctagttttgg tccgtaaaag gctgcctcgc
     1621 cttctgcttc aaagtaatct agttctaatt catccatagc ggctttcagc attgcttggg
     1681 ctttttccca cattgcgtca tcatcaaaat atttatctgt attgtttgga tcgcgatagc
     1741 tcaagcggaa acgataatct gtaatattga agtcagcata tactgccacc atcaattcaa
     1801 gcgtacgttt aaattcatct ttgatttgat caggacgaac aaatgtatga ccatcattca
     1861 acgtcatttc tcgtacacgt tgtaaacctg acaaagcacc tgatttttca tagcgatgca
     1921 tcattcctaa ttcagcgata cgaattggta attcacgata agaatggatc gtgtttttgt
     1981 agaccatcat gtgatgaggg cagttcattg ggcgaagaac aagcatttcg ccatctccca
     2041 tatccattgg cgggaacatg tcttcttgat aatgatccca gtgtccagat gttttgtaca
     2101 actcaacatc tcccattatt ggtgtatata catgttggta acctagacta acttctttgt
     2161 ctacgatata acgttcgatc gtgcgtcgga ttgttgcccc ttttggaagc cagaatggca
     2221 aaccagaacc tacttctggg gaaaccatga acaagtcaag ttcttttcct agtttacgat
     2281 ggtctctttc ttttgcttct tcacggagac ggatatattc tttcaaagct ttcttgtcaa
     2341 aaaaggctgt cccgtataca cgttgcatca tttgattgtt tgagtttcca cgccaataag
     2401 cacctgccac tgataataat ttaaatactt gtatacggcc agtggatgga acatgtggtc
     2461 cacgacacaa atcgatgaat tcgccttgtt gataagcagt aatcacttct tcttctggca
     2521 attcactaat cagttctact ttgtacggat cacttgcaaa aatttctaat gcttcctgtt
     2581 ttgaaagaac acggcgttcg atcggcagat tttctttgac gattttcatc atttctgctt
     2641 cgattgccgg caaatcttct gcagttactt ggttttgtcc attatctgta tcgtagtaaa
     2701 accctgaatc gatagctggt ccaacgccaa agtggatatt tggatagagg cgacgcatgg
     2761 cttgggccat caaatgagct gctgaatgac ggactaagcc aagtgcatct tcatggtctg
     2821 gtgtgatgat ctcaatactt gcatcttcct cgattggacg agtcaaatca acgagttttc
     2881 cgttcacttt accagctaaa gctttttttg ctaagctttt tgaaatgctt tcagcaattg
     2941 ccaatgttgt tgttccagac tcaaattctt tgactgcacc atctggaaaa gtaattttta
     3001 tcataatagt ttcctccttg aaatgttttg attgatcaga tcatcaagct gaaaaacaaa
     3061 aaaatcctag catctcttga aaagagatac taggacgaaa agttcgtggt tccacctaat
     3121 gttcaaacgc aaatgcgttc ctcaagcgtt gtaacggtcc gaccgccctt gcttcaacaa
     3181 gggtttcgaa agtggtcatc ctttggtttt ctcctggaaa agtttcagcc tagcttttcc
     3241 tctcttatcg aagactctca aattcagttg gctttctcat caatcaatta tgtgtttatt
     3301 taaccactaa taaatagaaa aatcaagtgg gaaactaaac ttattttttc cattccccat
     3361 ttacaccgta tgttcctttt ttcatctcat tgatgatgac atggatgtgt tcttttggtg
     3421 cctgcgtgtt tttatgaaca gcttccgtga tatctttcat catattttct aattgttccg
     3481 gactgcgacc ttctactaat tcgacatgta cgaatggcat aatgctctct cctctttctg
     3541 atcgttcttt tttcaactcg attctatcat actatgtttg tcttgggtac tgaatcagga
     3601 aaaatggctg gatctttttt tgctctcaag gcactcaaaa tagagaccgt atccacgact
     3661 tcttgcaaaa cagctccgaa taatgcgggg attagtcccg tacttgcaat taacatcaaa
     3721 aaaatacaaa taaagatgcc aatcagtaca gactgtttcg ctattctcat cgtatctctt
     3781 gaaatcacta ctgcttttgc tacacgcaag agatcatctt tcaagatgac cgcatctgca
     3841 ctttcactag cagctgtaga accatgagcc cccattgcaa taccgatatc tgcagcagca
     3901 agtgctggtg cgtcattcac gccgtctcct accatcgcaa caggtctttg atctttaggg
     3961 atactttcta aaatggtcag tttatcttga ggcaagctac gggcatgtac agaatctaat
     4021 tggacttttt ctgcaatcgt ttgagcaatg gcagatccat ccccggtgat catcatcaaa
     4081 tttttcacat caagttttct tagctccttg attgtctttg ctgcttctgg gcgaatctga
     4141 tccgaaaata ggatcgttcc tgcaaaagat cgatcaattg ttacatagat tgctgtattg
     4201 gacgtttcta tttcttcagc tgaaacaaaa gaggcacgtc caatctttac ttcatgatta
     4261 ttgactactg cttggattcc ttgaccggtc acttctttca gttgactaac aggatacagt
     4321 tggctgtttt tcacgttgtt cactaacgaa cgagctaaga tatggctaga ttcctgttct
     4381 gcactagctg catacagaag tagctcttct ttttttattc cattgactgg ctgaatcgcc
     4441 tctacttcca atatcccttt ggtaagcgtt cctgttttat caaaagcaat cgtttttgta
     4501 cgtgcgagct tttcaatcgt tgttccagtt tttacaacga tcccgttttt acttgaacgg
     4561 ctcattcctg caactaaagc aatgggcgca gctagaatca gtggacaagg tgatgccact
     4621 accagtacct cagcaaatcg taaagggtct tttgagaacc accaagaaat accaccgatc
     4681 aaataagcaa tcaacgtgaa agggactgca taacgatcag ctaaacggac aaatcgggca
     4741 ggcttttctt ttgattcttc gactagttta ataatcattt gatactggct gtctgcggca
     4801 cgtttttcag cgatcatttg gatcgatccg tctccattta aagaccctga cataatctga
     4861 tctccaggtt tcttggtttc cgggcgggac tcacctgtca aagaagattc atcgaccgaa
     4921 gattctcctt tggtcactcg accatctgcg ggtacaagtt cgccaggctt gactagtaac
     4981 tggtcatcaa tatttatttc atcgattact atatcctgca attttccttc tttgaatcga
     5041 tgggcaattc ttggggtgtt atctaacaaa gactgcagtt ctttacccgc tcgtttattc
     5101 gcgtaatcct ctagactatc tccgcccgta agcatgatca atatcatcaa actagcccag
     5161 tattctttga cggctaatgt tgcaaggatt gcggtaatcg ctaatacatc tacgccatat
     5221 cttcctgaac gtaacgtctt gatcatttcc aaaaacatca agaaggcgag aatccctcca
     5281 gtaaatgcga caatcgcaaa tgcaagggcc ggctggtgaa aaacaaattc acttaataaa
     5341 gctatcccac caataagcaa agtaagtaaa aattttttga aatgtgtcat tgttttttct
     5401 cctcttttat gtgacttaga gaggttagta ataaatttcc agctgcctct ttcatcttaa
     5461 gcatagtcct tgactagcac tttttaaaac aataggcctt aattgaaaac aactatcact
     5521 atcagcgttt ccaaaattca tcagttctac aaacaaaaat attcagcaag gattactttt
     5581 tcgattcatc agttaaaaag tacctttttt cgaaaaacta tgctatactt ttaattcgtt
     5641 atttacggcg gaaggagcaa acatgcgatg aatgagaaac aactagaaca aacgcacgtg
     5701 gatgaaacga tcgaactaat ccagatcgaa caaaatatgc tggatacaaa acaacaagaa
     5761 ttgactcagg aaatgaatca ttcgacaaaa gacacagcta atcacaagat tcgagctggt
     5821 tccaatgaag ctttttatga atctgctgtt gaatatcgcc aacatgagca ggaattgctt
     5881 ttgaaatacc atactcttga agcacagcaa aaacgcctgc aaacgttgtc tgtcatgaaa
     5941 gatagtcctt attttgcccg tatcgacttt aaagaagacc gtgaaacaga aacattatat
     6001 ctaggtatcg cttctttaag agatctggca gaagaaacaa tcgttattga ctggcgcgca
     6061 ccaattgcca atctttatta tgaaggtgag attggtcccg cattttatga aactgaaaca
     6121 gatagaatag atgtcgatct gcagttgaaa cgacaattta aaatccaaga aggacgtatt
     6181 ttatctatgg tagatacgtc ggaagtaatc aatgatgact ttttgctaga gatattggat
     6241 gaatcatcaa gcaaccatat gaaaaacatc gtctctacga tacaaaaagc acaaaatgca
     6301 atcattcggg acacaacaag taaagtaatg ctgattgaag gaatcgccgg aagtggaaag
     6361 acatccgctt tgcttcaacg tattgctttt ctgttgtatc ataatcgcaa gtggctcgag
     6421 gctgaaaacg ttcttctatt ttcacccaac catttgtttt ctgattacat ctcgactgta
     6481 ttgccttcat taggtgaaag cggcgtgcct acacagacat tcaaaaatta tttggtgcag
     6541 ctgctgccag agttttcttt actagatgaa cagcaacaag aaagtggttt tttgctaggg
     6601 gaaaaagatc cgatacaagt gatgaaaagc ggattgacct tagtcgatca gattgatcgc
     6661 tatatccaag gcatcacaaa ctacggtccc ttgttccgtg atatgaaaat aaatggccgt
     6721 acgatcctat caaaggaatc gattcgccaa tggtataaag aaacaaatga acaattacct
     6781 ttacatcata gactttctct attacaaacg aagctcctga aaaaattagg cggtctgcaa
     6841 aaagacgaaa cacgtcagca atgggtcaaa gatctggcgg aagaacagct ccaagaacta
     6901 tacgcaacag atccaaatct tgaatataca gaacaaaagg agcgttcatt acgtaaaaaa
     6961 ctggccaacc aaatcgtacg taaaagattc cgtcgaatcc atcgagggat catgaattat
     7021 caatttgtca atataacgaa acaatatctc cactttctac aaactgtgcc actttctctt
     7081 ctaacacgac ataaaatcag cgagaccagg tggcaggaac atttgacaac tgttcgtaca
     7141 cacttgcgtc aaagagaatt gcgtcaagag gatgctgtac tattcttttt actgatgcgc
     7201 cgaacatatc cagtgacggt tacacacaaa gctcgctata tctttattga tgaaatgcag
     7261 gatttcgcac catcacaagt ggcattgctg caagccttgt tcccaaaagc aaatttgact
     7321 ttctgtggag atctaaatca aaatgtcttt ggaaatgaaa cgatcgttgg atcattggaa
     7381 gaccttttcg tggaccgtga agtcactcgt ttccaattaa cgacaagcta tcgctccaca
     7441 aaagaaatca ctgattttgc taatcatttt attgtgaaag aacatcaagt agaaacaaca
     7501 gcaagaaaag gagaccttcc tttgctcatc caaggaacaa cagatgatat caaattgaat
     7561 tggttagcag ataccattcg aacgactcag ggaaaagccc ggtattggcg tacagcaatc
     7621 atagctaaaa cacaagaaga atgcgaagct ctttatgctc aactgccaga cgagttgaaa
     7681 caacaagtcc agctgatttt aaatgagagt gactttatga agcgttccat tattttactt
     7741 ccagcctttt tagcaaaagg tctggagttt gaccgagttt tcttgtggga tgtagatgca
     7801 gaacattttt cttctcagca agacaaattg atcctttaca ccatgtgtac acgtgcgatg
     7861 catgaattga ccctttttac ctccaataat gaaagtcctt tgttaaacaa tatggatatt
     7921 tcatcttatg agaaaaaagt actgtaagta aaaagacaac tgctatcatt aatgttagca
     7981 gttgtctttt tatttacaat aataattgca gaaaaagtaa ttca
//
LOCUS       DAZUKZ010000112         7835 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000112 DAZUKZ010000000
VERSION     DAZUKZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.115"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..995)
                     /locus_tag="ACG8EF_002346"
     CDS             complement(21..995)
                     /locus_tag="ACG8EF_002346"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS4947629.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="ACG8EF_002347"
     CDS             complement(1075..1950)
                     /locus_tag="ACG8EF_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFS4947630.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="ACG8EF_002348"
     CDS             2080..3987
                     /locus_tag="ACG8EF_002348"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFS4947631.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="ACG8EF_002349"
     CDS             complement(4528..7806)
                     /locus_tag="ACG8EF_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFS4947632.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2208 a         1658 c         1460 g         2509 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaag
//
LOCUS       DAZUKZ010000113         7720 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000113 DAZUKZ010000000
VERSION     DAZUKZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.116"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..2403
                     /locus_tag="ACG8EF_002350"
     CDS             76..2403
                     /locus_tag="ACG8EF_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFS4947633.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2400..3314
                     /locus_tag="ACG8EF_002351"
                     /pseudo
     CDS             2400..3314
                     /locus_tag="ACG8EF_002351"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            3387..3902
                     /locus_tag="ACG8EF_002352"
     CDS             3387..3902
                     /locus_tag="ACG8EF_002352"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFS4947634.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            3899..4345
                     /locus_tag="ACG8EF_002353"
     CDS             3899..4345
                     /locus_tag="ACG8EF_002353"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS4947635.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4495..5310
                     /locus_tag="ACG8EF_002354"
     CDS             4495..5310
                     /locus_tag="ACG8EF_002354"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4947636.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5516..6688
                     /locus_tag="ACG8EF_002355"
     CDS             5516..6688
                     /locus_tag="ACG8EF_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4947637.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6850..7557)
                     /locus_tag="ACG8EF_002356"
     CDS             complement(6850..7557)
                     /locus_tag="ACG8EF_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947638.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQALP
                     ENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAI
                     AQQQAEQARQQAAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATL
                     SEAQARGLTPCSKCYGN"
BASE COUNT         2606 a         1453 c         1534 g         2127 t
ORIGIN      
        1 atcaataaaa taataaaaat taaatttaga ataacgtgta aaggattatt tgatgatttt
       61 ttattgagag gagggatgaa aatgattaag aaaactcaaa ataaaatcca acgtatatgt
      121 caaaaacatt ttgtttctag actcaaagct gtatctaaaa gaaaatactt acatcccatt
      181 atgatttcga tcatcttgat ttttactttc ttagggagct tatcaagtat ttatgtaaac
      241 gtttatacag aaacgtcaaa ttttgctaac caaacgacag aggagacaaa tagtccaccc
      301 tattctgaaa tacagtccat agaaagcacc aatcagtcta gcaacaacga gacaacctca
      361 accacatcct cgactcaact gattgatccg acagaaaaag aaatcacagc agcaaaagca
      421 gcaggggaga aagcttattc agaaacaggt agaagccaga catttagtac tgttgcggcc
      481 actgcgaatt tcaacggaca taccatacca gatggatata cagtgatata tgccaaagat
      541 ggagtcattg ttgcggttaa cggatcgatg agtgcggata aaactgcgtt acaaggcgta
      601 accaaagcaa tcgcttttat tgataaccgt ccagatatcc ataatctgtt tattggctcc
      661 acagtaaatg gtactactat cacattccca aaaagtatct atatgggggt ggctaccgct
      721 ggaaatacga tcgattcagt tacacccaat gcggataaaa gttattcttt tacaccctca
      781 acatggggag ggcttcctgc cggaggaggt atgagtactt tttcaatcgg tatacagtta
      841 gcaaatgata ttccattcac ggggtactcg atcaaaaatg ggcagatcgt tgatggtaag
      901 ggaaatgttt caaattttcc aacctatcaa gcagcaacgg ttcaggtcaa tgcgaatcgg
      961 acaggaactt tttcttatac agtcaatggt ggagcagcaa aaatcggaac gtacgggact
     1021 acattatctg gaatccatgt gggtgacaaa gtggttatca ctccaacagc agtaacgggc
     1081 tattcttttg cacaaacaaa cggttctttc acagccctag aaagtaacgt cgatacggtg
     1141 acctatacag gcaaacaaac accaatcact attaagtatc aagactattt tggacagatg
     1201 attgctcctt ctaaaataat gaatcttact tacggaagtg ctgcacaaga cctgacgact
     1261 aatacgccta ctatcaacgg atataccttt acagctgttt cagcgataga aacaaagaac
     1321 caatcagcaa tgagtgtttt agctagttta gatacaaatg gaaatgtaat cgtaaaggac
     1381 gcaaatggaa aacagatttc tgaagtcatc ctatattata agacaaatgc aacagtatca
     1441 atcaatgcaa atggcagtaa gtactatgat ggtctttctg ttgtaccgat cgttaaatat
     1501 gcctttaatg ataaaacaga atcaacatcc cttttaaaca atgatctcag tattgatcaa
     1561 atcacatgga atccaactga tttcaaagcg atgaatgaaa agggagttga agtaactgcg
     1621 cctactgaaa ttggaaccta tacgtcttgg caattgacaa aaagcggact agctaaacta
     1681 gctgccagaa caaactattt atttactatc gtgcagacat ctgatgtcta tacgattaag
     1741 caaatcagcg gtgcggtaga gctcagtggc agtaagacat atgatggaaa agcaggtgtc
     1801 ccgtcaatcc atgtcaagct agcagagggt gtcactagtt cgcttactcc agtacaagtg
     1861 gcacttagca gtatggatta cacagcagat gcacaatcag caaaaaatat ggttaatgct
     1921 ggcagctata caattaaact aacgaatagc ggaatagaca aagtcaaagc agcaaatccc
     1981 aagttgtcat ttactgatat agccaacaca tcaggaactt ataccatcta taaagctaac
     2041 gcagtgatta ctgtcgatga cgctgccttt aattatgatg cccaaagtca ctcgatttcg
     2101 gttggaaatc tccacgttac cggtgttgtc ttaggcgaat cattggatta taccctaaca
     2161 gttaacagcc ggacagatat tggatcacaa atagtaacga ttagtttagg cgaaggaagt
     2221 gtaaacaata attatacgat tatcgcaaca gataccgcta agttgacgat caatccgact
     2281 ctcttgcttc cttcgacagg tgggataggt ctactgccct ttgtcttttt aggactgatc
     2341 tttatttttt ctggcttctt ttattttatc catcgtaaga aaaaagcagg tgaacagcga
     2401 tgaaaaaaac aaaaaatgta cgtgtggata tatttgctgt tctctttttt atcctactgg
     2461 tcttttttat aggttctccg actgtgtcag ctagagaatc acctattaca tttactttgc
     2521 ataatcgaat gttcgaaagc gataagatgc caagtatcag tcaaaacgac ggaataaata
     2581 tcccggataa ctttcaagga gtaaaagggt tgaacggcgt tactttcgct atatatgatg
     2641 tctcggatga attttacaag ctgcggtcag aaggaagtag tgtggaagac gcacaaagaa
     2701 aactagcaca aaaaagtgat agtgaaaaaa tactggcaga aaatgtgaca aaaacagtag
     2761 acgaggaaga gggcattgct tcttttagtg catcggacaa agatgagcaa ggtcgtgacg
     2821 ctgtttaccg atttacggaa ataaagactt cagattaagt aaaagaaaaa tcagcaccat
     2881 ttgtcgttgt gcttcctgtg acagattcat ctaatcataa attgacgacc atccacttgt
     2941 atccaaagag cgaacaaaaa atacctgctg cactcacatt aacaaaaaca gtagagaata
     3001 agcagacaga ttttgcagat ggggataaag tcccttatct catcaccgta acaattcctg
     3061 agaacatcaa cgagatagga acctatacga tcaaagatac agctgatcct cagttatggc
     3121 tagaaccaga aacgatcgat tttttgattg gtggagacaa gattcataca tttcacacac
     3181 aaaaaataaa acatggtttt gtcttatcag attctggaaa agatcttagt tcgtttgctg
     3241 gaaaaaaatt aacgatccgt taccaaatga cattgaaaga aaacagtgaa aaaacgacat
     3301 ttgataacga tgtacgtttg acgactgaca atcaaacagt agaaacacat cttgccatcc
     3361 aaacaggagg caaacaattt gtcaaagtgg atcggcaaac gaaacaaaaa ctagcagatg
     3421 ctcaatttct agtcaaaaaa aatgaccaat acttagcgca agaaaacggg ataaatcatt
     3481 gggtagccaa agatgattca agagcaacga tttttacttc cagtaaagat ggaacatttt
     3541 ctgtccatgg attgtctttt ggaagttacg aactagtgga gattaaaccg cctaaaggat
     3601 atatattgag tcaatcaacc attctctttg aagttgaaaa aggcagttat gatccgcttc
     3661 atcctatacc attggaaatc atcaacgaac caaaagcaaa tccgacgaat aatggaaaaa
     3721 cgccagttca gatgcagaca aatggtccat caaatgaaaa aaatggagga agctttccaa
     3781 aaacaagcga agagatgctt ggaggtttct caattttagg gttattgcta gtcctttcaa
     3841 cagggacagc ttggttttat aaaaaacaac aaaaaggaaa caataggagg gaaataaaat
     3901 gaaaaacatc aaaacaaaaa tatggacgtt tttaggaact gctatcatgc tgcttccttt
     3961 cgttctcggc ctaggaactg cagaggtttc tgcagccgta tcacctacac cagaaaatgt
     4021 tacggttaat ttgcataaat tgaaattcac ttcagcacca gaaaatcaaa taaacaacgg
     4081 aacggaattg acatttccga atagcgaacc gttgaacggc gttgaattca acgtatatga
     4141 catcactgct acgtactatc caagtaaaga cacagcagtt ccagcagacg ctacaccctt
     4201 cgcaagtgta acgacttctg gtgaaggatt agctaacctg actcttccag gtaaatctga
     4261 tgggaaagat gctgtatacg tctttgtaga aacaccaaag cccggtgtcg aaacgtcgcc
     4321 aaatatcgtg ttaagcttac cctagaccct gacaaacctt tagacacgat tcacctatat
     4381 gctaaaaatg tcatcaaaga aagcaatatc aaaatccaga aagctgggaa cttttcaggg
     4441 gtagataagt tagcaggggc gcaattccag atcaaaaatt cagagaatca attcgtgacc
     4501 ggttttgatg aaaacactgg tgtcgctctc tatgatcctg ctgagtcagc gttgacattc
     4561 acaactgata ctgaaggatc catcagtgtc aatggattaa tagacggcaa ttatacttta
     4621 gttgaaacaa aagcaccaga tggttatcaa ttaaccaatc cacaaaaaga aactactttt
     4681 atagtaaaaa acggaaaatt gcaacaaaca accggtatca tccatggcgc agatgaagcc
     4741 aatcttggga atccaaacgg tgtagtcaat cgggaaaata caactacgga taacacgatt
     4801 actgtagaaa atgtccaaaa ctacggtgat ttccacttca tcaaacaaga tgtgaacacg
     4861 caagaaaaat tagccgatgc agaattttct attctaagag caaaagatcc aactgttaaa
     4921 ttaatgaaaa aaatagcaaa ccttgcctca ggagaatacc catatgcttg gtcagatgaa
     4981 gtcacaggtt ctgactggga agaagtgaaa ttgatttctg attcagaagg gaaattttca
     5041 atcgaagggc tgaaacaagg ggcatactgc cttcaagaaa caaaagcacc agaaggctac
     5101 gcattaccta agaatgaagc tgcgtacact gaatttacag tagatgatga tccaacaact
     5161 tctgatgaaa caacgatcga taaccaacct aaaggaatct tgccttcaac tggtggtatg
     5221 gggatcatcg tattcatctt ggtaggtact gctttagttg gaggagcagt gatctatttc
     5281 aaaaaacgcc atcaagaaac agaagcttaa actattggaa acaaaaatca tacgataaat
     5341 aaacgatagt gattcaaatg gggagaaagg ttgtgacaag tggtcggtct cttggtattc
     5401 gctcaacaaa cggcaaaata ggaggaacta ttttgccgtt tgcttcgcta atactcaggt
     5461 caaggcgctg atttcacaac ctcttcatat acatattaga aaataggtgg acatgatgaa
     5521 taaaagcaga ataagaatac tggacatctt catggcaatc attttagtcg ttgggatcgg
     5581 gatcttttct tatccttttg tagaagattc actgaatgat tttcttgccc agcaaatgat
     5641 catccattat caaaagcaag catcgaataa gaattcagag gaaataaaaa aacagcagga
     5701 aaaaatgaca aagaaaaacc aacagttggc agagcaaaac gtatcaccag ggataggcag
     5761 tttcaaccaa gcagttgatg caaaagcatt aaaagattta cctagtaatg ccttttttat
     5821 ggaacatatg cttggagtca tcgaaatccc aaaaatcaat gtcagcttac ctatattcga
     5881 tcagacaacg gaaatatttt tgcaaaaagg aacgtcatta ttggaaggaa gttcttatcc
     5941 gactggcggg aaaaacaccc atgccgttct ttctgggcat cgtggactcc cagaagcaaa
     6001 attatttaca gatttgccta agttgaaaaa aggagaccag tttttcatcc agatcaatgg
     6061 aaagacattg gcgtatcaag tagaaaaaat ccaagtggtc ttacctgacg aagtagattc
     6121 tttgggtatc caaaaaggca gagatctcgt gactttgctt acctgcactc cttatatggt
     6181 caatacacat cgactgcttg tgacggggca tcgtattcct tatcaagcaa aagaggcaaa
     6241 aaaagcgatc caaggaattg atcaatggaa aaaatggaaa ttttttattt ggtttatcgg
     6301 tatactactt ggatcaatcg gcttagtttg gctactcatc gcttatttag attctttggc
     6361 catcgctaag cgaaactatc ctttatcttt ttatgtaaag aacacaaacg gacgaccaat
     6421 cgaaggaatg gtcttttctg taaaaacgct caacggcaaa cactatatta ctagagaaaa
     6481 agttccattt gtgaaagctt ctgatgaata cggactagtg agattttctg atttaaaagg
     6541 caggaactat cgcttgcaac atgaggagct cctgcttaaa atccatgtaa aacataaaca
     6601 ttcgaaacag ttttcaatga aattgaaaaa aggaagatat aaactgcgaa aagaaaaaga
     6661 ggtgtactat ttgattgaga aagaatgaat gaagatgtcc aaaaaagcag agggaacaga
     6721 ctaaatgaaa aaagagagaa gctgacgaaa tggacgtgta aactgctatt tttggtttac
     6781 tatctggaac gccagcttct atttttttat gtttcagaag aattttatta ttatagtata
     6841 acttttgaat tagtttccat aacatttgga gcaaggggtt aaaccacgag cttgagcttc
     6901 gcttaaagtg gcaggggtat acgtaccatt tccacattta tgtgtatggt attttgaacc
     6961 ggtcggtgtg acatagacag tttgttcatt attttgctca ggagctgcgg cttgtgcctg
     7021 ctgagaagcc tgttcagctg ctgcttgctg tcgcgcctgt tctgcttgtt gctgtgcgat
     7081 tgcttcttgc ctcgcctgtt ctgcttgttg ctgtgcgatt gcttcttgtt gcgcttgttc
     7141 tgcttgttgt cgtgcaattt cttcttgctt tttccgttct tcttctgctt cttttgcttt
     7201 gattgtggaa tcaattgcag caagacggct tgtcaactct tgtttgtttt caggtagcgc
     7261 ttggatagct gctgaagcag ttgcatagtt ttcttttgta ggatttgctt cagcttgtga
     7321 caaagcagat tccgcttctg tcactttttt ttctagtgca gctttttccg ctttttgttt
     7381 ctcttcttct tttttctttt caatttgttc tttcgatgaa tctgccgtac tttcggagat
     7441 cgtggtactg ctgctggaag cctgagctga acttgtgctt tgagtagtag acgtagtagc
     7501 accacaccca ctcaacaaac ttcctgcgac caaaaacaac gcaactaatt ttttcactaa
     7561 atttcctcct taagtaaaaa acaaccgttt tcaataaatg tccttttcaa ttgtttactt
     7621 acttgttata ataattgtgt tatgaatagt gattgaggaa acttaatcta atcccgtgtt
     7681 gatacacggg attttttgct atagttctgt tattaagtat
//
LOCUS       DAZUKZ010000114         7617 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000114 DAZUKZ010000000
VERSION     DAZUKZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.117"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..787)
                     /locus_tag="ACG8EF_002357"
     CDS             complement(59..787)
                     /locus_tag="ACG8EF_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4947639.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(800..2080)
                     /locus_tag="ACG8EF_002358"
     CDS             complement(800..2080)
                     /locus_tag="ACG8EF_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS4947640.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2221..2823)
                     /locus_tag="ACG8EF_002359"
     CDS             complement(2221..2823)
                     /locus_tag="ACG8EF_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS4947641.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3068..3274
                     /locus_tag="ACG8EF_002360"
     CDS             3068..3274
                     /locus_tag="ACG8EF_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947642.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3586..4305
                     /locus_tag="ACG8EF_002361"
     CDS             3586..4305
                     /locus_tag="ACG8EF_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4947643.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4386..5069)
                     /locus_tag="ACG8EF_002362"
     CDS             complement(4386..5069)
                     /locus_tag="ACG8EF_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFS4947644.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5287..7473
                     /locus_tag="ACG8EF_002363"
     CDS             5287..7473
                     /locus_tag="ACG8EF_002363"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFS4947645.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2727 a         1283 c         1436 g         2171 t
ORIGIN      
        1 agaaccgaag ggtttggatc ggctggtcat ttagtcggtc caaactattc ggttcttttt
       61 atgaaatcaa tttctttaat ccacggatag aggctgggat ggtctcgccg tttgccaacg
      121 tgacttctct ggttttttta ttaatcgacc gcacattttg tgtattgata acataggatt
      181 tatgacaacg gataaaattc cgatgcattt tctcgatttc tttgatcttc tcatagaatt
      241 gtaattgggc atgagtcaaa tggacttcca gtttatgtgg cgctcctagc gaagtagaaa
      301 aatattggat gtctttgatc ggtaaaaatt tgatctcatc attaaactct attttgaaga
      361 tatctttttg gatttgggac atttgaaaat cttcgaaaat tttgctcagg atatgctcga
      421 cattttcttt taaatcataa agatcttctt tgacgatata atcagtcggt tcgatgttgc
      481 tttttaagat tttcatcgac atttccgtat gggaagtcac atagattatt tttcctaaag
      541 gatctaattc acggatcaat ttaccgatcg ctacaccatc catttcactg tcttctagtt
      601 cgatatctaa aaagtaaata ccaaaagaag aagttctgat cttggctgca ttgagaagtt
      661 cttccggatc agaggtagaa atatggacat aactgtctaa gttttcaatc atgattctat
      721 ttttgatcac cgttgataaa acttctaatt gttttggatc gtcttcacaa atataaatgg
      781 gaatcatatc attactcctt tattttattt gtatataaat cgtttgagaa aaacgctggt
      841 tttcaattga tgtttcaagg aaaagataag gattggtctt tgtcaattca tccaagataa
      901 acaagccaag gccttcgttt tttggatttg tttttgttga gaaccctttt tgtttcaact
      961 ggtttaacgg taatgtattt gttgttgtat tgatcacacg aatcatcacg gaatgattct
     1021 tcttgagtat ggtcaattcg atttttttct tctttgcagc actagctcct tcaatcgcat
     1081 tgtccaagat aataccaagc atacgtacaa ggtttaccgt atgtttcgta tccagttcga
     1141 ttggctcagg aaaatgaagc tggacgatca accctttttc ctgtgccatc atgagtttta
     1201 atgacagtaa actacggatc tcaggaactt tcatattatc caacatactt agattggccg
     1261 gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg atcataataa gaccgtaatc
     1321 cttctaagtc gccttctgaa atatagctgt gaagagaaaa aagcagattt tggtaatcat
     1381 gtttgaattc tctcaagtct tgatagtttt tttctagatc tttgatataa tcctgttgca
     1441 tcctgacttg ttgctctaat aactgatcag ctaatttctt attagagatt ttccaataat
     1501 aaaccaagat gatcaggcca acacacagat atagaatgaa aaaactcgta ttcaaagcta
     1561 agacatcagg tgtttcacca gaaaaacggg tataaaaaat aatgatatag tatgtaaata
     1621 atatcaatgt cccgatcgta ccgatcatac ggcggtccac agtgtgaaac caagtctcta
     1681 acgatttttt taaacaaaaa gctaataaag cagataaaaa agcggataag acaatgtgaa
     1741 aatactgcaa tgaatctcct ggttcaataa ccgaagattt caaaacaaaa aaatcgtcga
     1801 tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc aaaaggaagc gcgtaaacca
     1861 attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat cagcattcct atagcaatta
     1921 gagcagtaaa aatccctaaa atcgaataga cccatccaat caaaatacaa gaaatcagaa
     1981 tgactcccgc aaacagatat ttgtatcgga tgtgatggtc aatcaataag atacaaattg
     2041 tgtaaatcaa atattgcaac gctatggacc ataaaaacat aaagtacagc tcccattgat
     2101 ttttaccaaa ttatagcaca attagcgtat tattttttta atttttcagg gattttgaat
     2161 tcagaactca taccaaagca gcagccagat acaaaaaagt cagataagtt agttaattgt
     2221 ttaatgattg tttttttcat gaaagattcc tccaaaattc atcaagtagt ttgtaatagg
     2281 aagcaacgta gctatttcta aaacaatccc aagtagcaaa taggtagaaa agtaagaagc
     2341 tgagaaaaat aaagtaatga gagaaaaaat tagaaacatg ctgatcgcct tctttaataa
     2401 tgggggacgt ttctcgggtt caatgggatt gattgctgta cctcttgatc catagcgaag
     2461 cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt gggaaggaga tagtcaattg
     2521 cagatgtttc atcaccaagg gaacgcccca taaataaagt agtgtccaca gcagacaaac
     2581 gaattcagag tcggaatgca atccataagc atatctgcgg atgcagatat aagaaagaaa
     2641 ggtgattcct gtttccgccc atgagcctgt caccagagaa aagactagaa cgaccgctag
     2701 ttttccaaga ttattcaata aaacttctaa ggcaaatttt acttggacat acaccatatc
     2761 ttcttccgat ttgtctccgc tgaataaggc ttctaataat ttgttcacaa ctttatcttt
     2821 catctcatac gtacctccac cacaaatttt atgctaaaaa aaataaaaaa cgtcttttgt
     2881 ttcataaaaa gtggttttga catcaaaacc gtcatgatct cctaaaaaaa tcttcgtttt
     2941 ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa aagtactttt tatgacaagt
     3001 ttgtgctttt tcgagtgatt tatgttaaaa aattactgac cttataaaag gtgatggagg
     3061 gattcttatg gaaaatgaac aattgtttga agtcggcgaa acatatgcat ttaaatctga
     3121 tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc tatgaaaata gtgtgatgat
     3181 acgtgtcatg aaatgcgcag tagaagacaa tgataccgct cataatctgc actggcagtt
     3241 agttgtacgc aaatcaagta caatagaaga ataaaaacag ataaaatgtt gagagcataa
     3301 agaggttgtg agtctcccgt ttagctctga gttacaagga ataatgttaa aaaacagctt
     3361 ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg caggagaata ctgtttattt
     3421 gaaaaaaggg ctgcgacaag acttttgtca cagcctttat tttcatgaat aggagttact
     3481 tttggctact tttagagaag tagcttttaa ggagcagata aagtcaaggc ttttatttct
     3541 ataaatgaag tagtaaaatg aagaacagat tttattacag gaaggatgcc gataattgtg
     3601 atcattgttg caggaacgat cggtgcaggg aaaagcacac taactgaaat gcttgcccaa
     3661 gatttagaaa caaaaccttt ttatgagaac gtggaagata atgaagtatt gccgctattt
     3721 tattctaatc cagaaaagta tactttttta ttacagattt tctttttgaa caaacgcttt
     3781 ttggcaatca aagatgcttt cagccatgat gacaacgtac tagaccgctc catctacgaa
     3841 gatagtatgc tgtttcattt gaatgcagat ttagggcgtg tttctgaagt agaagtaaag
     3901 cagtatgaag gtcttttaga aacgatgctg aaggagttag aagaaatttc gccacaaaag
     3961 aaaccagatc tactggttta tattcgtgta tcctttgaaa ctatgttagc gagaatcaaa
     4021 aaaagaggcc gggaatatga acagctagaa caagatcctg aactctattc ctactataaa
     4081 gaactcaatc gacgatacga agagtggtat gaacagtttg atatatgtcc caaaatagtg
     4141 atcgatgggg acaaatatga tttcgtagcc gatccggcat gtggagaaca aatcgtacaa
     4201 gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag atgcccagtc gatccgctac
     4261 actacaaata acccgaaagc aaagggaatc tttacaaata aataatataa aaactaagca
     4321 aattgctcac tcgcagatat ttctctgtga gcagcatctt gcttagtttt tttgattcat
     4381 tgagatcaaa tagtgaaccg attcgtatga taaaattgcc ataaatgaaa tgtctgagtg
     4441 agggccatga tcaaaaataa aatggaaacg atcaaaatcg aatgggcgcc aaacagatga
     4501 tgacagaaaa caaggacaat agctcctgcg ataaaggcaa gaacgacact tagatagtgg
     4561 aaaaatcgat caaaatctgc tttttcttta gaaatacaat aatcatataa gttcgttgct
     4621 acccctttta aatttccagt tgtaaataaa ttagtgtagg cacgcccgtt gattttatca
     4681 aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa tcgttactaa tgcgggatgc
     4741 tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg cttcataata tagaacaaac
     4801 aagcgccaga aatgtttttc tttttttcta aaataatcaa ttagatactt tgttagaaga
     4861 atccctaaga aaaagaaaag ggtagacaat acttttttac caacacttag ccattctccg
     4921 tcaaatgctt ggatgatcga tagaatgata ttgccagttt gagcagaggc aaaagcacca
     4981 tattggatat aagtataact gtccatggct cctccgataa atgtcagcaa aagtcctatt
     5041 gtacgcccct cgtgaaagga agtctgcatt tttttcatca ccttttatga atatttttgt
     5101 cttccttatc ttagcataaa cgattgaaaa aaaggaaaaa ttttcagaaa actaaaatca
     5161 tatcgcttcc cattttcatt tttagaaagg tctgttagta taaagaaaaa atagacaacg
     5221 aaactttaaa taaccaactt gaaataaaca aattcaaaga agaaagatgg gaaggaaatc
     5281 aatcagttga atgaaaaaga aattctccga ttacaaaaag aattgaaacg ctgggagaac
     5341 aagtataaaa agcttcaaca agtctttcgg ctacaagaag aaatcattgc tacctatgaa
     5401 gcggcttatg gcgaagcacc cggacttatt gaggaagaag tacaagtaaa agaaagtgaa
     5461 ggaaacagct ctttctttaa aatgatggag aaacgttcct ataatcgctt caatcaagaa
     5521 caaaaagcag ctatcacata tgatatgaag aaacatcttc gaatcatagc aggagctggg
     5581 agcggcaaaa cgcaaacaat ctgtgcaaaa gctgtttatc tgatgacgca aaaacaagta
     5641 gacgaagaac gcatactaat gatcactttt acaaggaacg ctgcaaatga actaaaaaaa
     5701 cgagtagata atttcagcca gagaaaaacc gctgtccata tcggaacatt ccatagtatc
     5761 ttttttagga tatacaatga aatttgtcga aaatttcctg aagtagcaat gcaaggaata
     5821 caaggagact tttcaaagga tactgcacaa aaagtgaatg ctgttttgca acagctgata
     5881 cgtaaatata atctttatat atttgatcaa tatggagaaa aaacgattgc ttctcggtta
     5941 gactattggc agaatatgaa cctttcagta gaagaaatgg ttcagttagt taaagaaaaa
     6001 tatgattcca ttgataagaa tgcacgacag ccgatatcag aacgactgta tggattgctc
     6061 acggaactcc aagaacaaaa aagaagccag caattgctag agttcaatga tgtactccaa
     6121 aatctgaaaa gcgctttgga gaatgaggag atccagcatt atatcggcca aaaatatgat
     6181 tatctattca ttgatgaatt tcaagatact aatccacttc aatgggaaat cgtgaaacgg
     6241 atgacaaagg aaaacaggat caagttgatt gttgttggag atgatgatca gagtatctat
     6301 ggattcagag gatcagagcc agcatacatc aagaactttg aaaaagaata tcctaccaaa
     6361 actcttttct tattgactaa ttatcgttct cgggcagcta ttgtccaagg agcaaataga
     6421 ttgatcagct ataataaaaa tgatcggata ccaaaggcga tgattccagc acaaaaagaa
     6481 gcaggtgtta tggaagccta tttgttttct gatactaaag cagaaagtca gtggctaatc
     6541 aaacaaattc agacatttat tcaaaaaaat gggaaatata aggaatcaat catattatac
     6601 cgatcagctt cacaaacgca gcagcttgtc caatctctgc tgaaaacgaa tattcctttt
     6661 gtactggaag cagatagtcc atatgaaggt atctttggta tcaagtcttt ccaacatttc
     6721 tatcaaaaaa ttgttcattg gcaaacagca ctgaatctgc aaaagaaaaa tcaggcctac
     6781 cagcaattgc tccggcagct gatggcagac tgctacctga aaaaaagtga atgcgatcaa
     6841 tacttttctc ctaaatatca aaatatagcc gttgcagatt atattttgtc gataagcccc
     6901 aatttaaaat cgaaatctgc agaaatccga gagtttgagg aagcgttgcg taaaacagac
     6961 cagcatcaag aaatctattc gcttgttcaa agctatcttc atctgccacg tatagctaag
     7021 gaaatcgacc agagtgatca agaatggata cgagaagaag taaagcagca tgctactttc
     7081 agaagactcc aagccttatc gcaagaaacc caagcgacag ctaaagaatt aaaaacccgc
     7141 ttgattgctt acagacaagg aaaacttgat gcactatgta tccagagcat tcacaaaagt
     7201 aaaggtctta gttaccgaaa tgtctttttg attggttgta acgaaggaag tttgccttat
     7261 aacggagcga cagaaaagaa agtaatcgac tttaatgaaa taaaagcaga accagtgacg
     7321 acgatagaag aagaacgaag actattttat gttgcgatga ctagagcaag aaatcgtctg
     7381 tatctatgtg tacctcaaat gaaaaattca agaaaattga aagtatcgag atttatcaaa
     7441 gaaaccggga gtcgtgtgaa aaaaggaaag tgatagaaga gagggtgtga cagaagtggt
     7501 cagtttcaag aaagaagaag gaatgtacga aaattgcttc tcaaattttt gtgaaatttc
     7561 agcttatttt cgaagaagct atttctggaa cacagtttat tcggaaaagg gaggtgt
//
LOCUS       DAZUKZ010000115         7061 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000115 DAZUKZ010000000
VERSION     DAZUKZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.119"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1778..2443)
                     /locus_tag="ACG8EF_002364"
     CDS             complement(1778..2443)
                     /locus_tag="ACG8EF_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4947646.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2569..4605
                     /locus_tag="ACG8EF_002365"
     CDS             2569..4605
                     /locus_tag="ACG8EF_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFS4947647.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4609..5247
                     /locus_tag="ACG8EF_002366"
     CDS             4609..5247
                     /locus_tag="ACG8EF_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4947648.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            5275..5703
                     /locus_tag="ACG8EF_002367"
     CDS             5275..5703
                     /locus_tag="ACG8EF_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947649.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            6160..>7061
                     /locus_tag="ACG8EF_002368"
     CDS             6160..>7061
                     /locus_tag="ACG8EF_002368"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4947650.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTA"
BASE COUNT         2419 a         1064 c         1305 g         2273 t
ORIGIN      
        1 caccccgttg ttatatgacc gtgttttgag aggaaccaat aaaaagcata ggctaactag
       61 gggttccttt ttcttttgtt tttttggaat ccgcttaagt gaaatgtatc tgaaatgaaa
      121 cggagataaa gtgatttttt gctataatga caaggtttta gctgtttcag ataccatgta
      181 acccaaaata gtttattgag aaaataggag agtatttgat actaactagt aggaaaaaat
      241 gagataaaaa ttaaatggaa tcatttcagg taatctcaaa taaaaaaata tacagtcctg
      301 tggcacttta cctatgggac tgtatatttt ttactctcag cgggcttgtt ttgactccgt
      361 gtctgactcc gtcgtattga ttttaaatga tattcgatga ccatttaaca ggtcattttt
      421 gaaatttttg tctctatatg actcagtttg ataattattg atagatcaat ttgggtctat
      481 atcgaacaag ggaataatcc aataaaaaat gatttagaat atcaaaataa caaaaaagag
      541 gactacctgt attcgtcctc ttttttgacc agattttgac cagtctaatt tacattctaa
      601 tatattcata tgagctaatt ttttgattaa atggtaagtt tttccttgtt tgttttagtt
      661 taaactagtg tgtaatttaa aaatctatat ggaagggtct gtggggtaat acttaaataa
      721 aggctaggaa cctgataaca tcaaggttcc tagccttttt ataataaaat gaagcgaaaa
      781 taatttcttt taaagagccg agattatcag ctatataaga gtgaatttga tgcaaaaaac
      841 agagccgaaa cagtcttggt gttcgtagct ctgcttatat gtttcgaatt taagacaaag
      901 agttggcaaa agatgttgat aaggaagata agataactgt gttatataaa ctaggtaact
      961 aactgtaaca agctaatttt aagagattct tattagaaga cgtttaggaa tataactaag
     1021 aaaatgatta tacaggtaat tgtcatttca ataatgtttt ctaattttcg cgtctttgtt
     1081 ttaagaaaaa ggccagaatc agttggaaaa aagttcgtac gatagtgaag aggggcaaaa
     1141 tttcctacta atattcccac taacaagaga taaaacggcc aggttggtgt gattcctcta
     1201 taaaaaaatg cccatgctac cgaaatccca tagattaaca actgaacaat ttttacaatg
     1261 aaaaaattgt tctgccatta ctcaaagtct cttcctcctt gtatcgttta tttgtttcat
     1321 tttatcatta tgataggcgc ttgaacaatc cattttgtat tttattgagt actcttggat
     1381 aaactaccag cgattatcat atttatttat gttactgtaa gccatcatat aaaagtaaat
     1441 gagcagatca tagaaatata aaaaagaact aaaattgatc taaattatgt gcagcttgac
     1501 tacgtaccaa tctatattac ttactagatt ggtcgttaat ctattcggat cgaatgagca
     1561 aaatattttt gtgaatgtcc atccttgatt atgattccaa aaagaatcat tgggaggctg
     1621 tggcgtaaat attatactat aagccagaaa tacctatcat tctgaacttt tttatttagg
     1681 aactttcatc tcaaggcttt ttcaattaat ttgtacccct gttttttgga taagatcatg
     1741 tgcattgatt ttttctatct ctacgttcat ctcctactta atgcctatct ggctcctgag
     1801 ggcttctttt taaggcgtcg tcatagtctt ggctgaggat ttcttggtaa tctgagggaa
     1861 tggcttccca agtgagcagg cgaacgttca tgacgtgttt tcctttgtgt ttgattacag
     1921 catggttttt ctctttttct acctctccag cgtgaaaaga cagccatgta ccatggccaa
     1981 agatatcatt acatacccaa cgttcgatgg aattgtccac gtatcctaca gggagtgtgg
     2041 tatagccgat ttgtggtttc aaaaaaggat tgaatcgatc gatcgtttca tttttgtatt
     2101 gataattgat aagaaaaaga tagaccaaac aaaagacgag cagaaagaaa gatagtgtct
     2161 ttttgagtga gaagggttgt tttgatgaat aagatttctt gttaacaaga ggttcatctg
     2221 gcaatgttgg ttctattgat tcggtagctg attcaataga aggaaaatca atcggcagac
     2281 ctaaagtatg atgtaaaagt gacagcattt caaggtctgg cattgtacga gaattttccc
     2341 aactagaaat cgttttatcg gaaacaccaa ttttttcgcc taattctttt tgcgtaagct
     2401 tacaatttct gcggtaagtt cgtatttttt ctccggtgtt catcttcttc tcctttcgat
     2461 tctacagtat atagatttat tctacatgac ctatacactt gatagatacg tttttttgat
     2521 gaaaaacggt atgattttat caaagaatca tttaagtaag ggagagtggt gataatcaaa
     2581 aaaaagttca cagttttact cacaatcata gtgaccggta tcatgagtgg ttttgccttg
     2641 gttagtataa ataaacaggt actctatcgg acaaatgaat ttttccatca aacagatact
     2701 tatactccga ttcgattgcc acaacatttt attgggcaag aagaatatga gcagctaatc
     2761 atggtatttg aaaatgtctc atcagaaact gagctttctt atatgaaaaa aacagtaaat
     2821 gaatccaatg aagtagatac atcttggaac ttcaagatac agccaaagct tgaaatgaca
     2881 ttgtattatc aaggggatgt caaatcttct cacgagaagg taccctattc taatacctcc
     2941 tttcgcgtaa cgtccattga agaatcgatc aaaataaaag attttgaagg tgaatttttc
     3001 ttgatgacga gagaaaaaga agcctatgaa cggttcattt cactattcac tgatcgattc
     3061 aatcaagcat ttcacactga ttatgccact aatatttttg aagactttga aaatagctat
     3121 tcgtattcgg ttatttcaaa tacagacgta tataattatc gagtgtattt aactattgga
     3181 ttgattcttt tattcctttt gttatcatcc tttttcttat tgatgaataa agaaatcagt
     3241 ttattgttga ataatgactt ttcattacat caagtattgt ggtgtttatt ggggaaaaag
     3301 aaaatcatta ttacattcat aattgcagga atcattggtg gttttatttt tatgtattat
     3361 aaggagcaat ttctttattt gttccagatg tacgttttct tgttcagcgg aatatatggt
     3421 atctctttga tttctctttt attagttgaa aaaatcgctt cgttaagaga agcgttcaac
     3481 tatatccaat acattctttt tacatttgga ttgttgtttt ctcttctttt gaccgttgcc
     3541 aatgtcgaat tagcaactat cgttttttct tcttcacagt tagctgtttc ccatgaacca
     3601 ccaaaagaaa tcgctggtga ggactatcat gtattttatc cagtaaccat cgggaaaaac
     3661 cacgtggatt ttatttattc caatcgtttt gcatcagcag cagatcagga attatatgaa
     3721 actttgaata aaaacggaag tatattagtc aatgtatcag attacttgaa tgaagaaaat
     3781 gaacagtttg gcagagggat aaaaataaat ctgaattatc ttaagaaatt tcctttgatt
     3841 gatgtctctg gaaggttgat ccaaataacc gaaaaagaaa cgcaccctgt tatattgatc
     3901 cctgaacgat atagacatac agatttaaaa aatgacctgg cgcaaataac agaatattat
     3961 caagaaatca gtgaaaaaga accgaaattt ctatttataa aaaacaatca acccatttat
     4021 acatttattc cttctatccc ctggatcgaa cattatccgg ttgttgaaat actgacattg
     4081 aaaaacagta tgtatttgga aagaaatatt ctcagcggag aaatctatcc accgttaaag
     4141 atcaagatag gctctttgtc gaaagaaagg ttatttgagc tgattgaaga aagtcagtta
     4201 cgtgataact tgcagttatc atttccgtat actcagactg aagagatagc tgtcaaagtg
     4261 ttaagcagat catttcatta cttgatacag atatttttac tcactttctt ttctttcttc
     4321 ttgttgagct atgtcatgtt atgcatatat tttgtctaca attgtcggaa aatcgctatc
     4381 tttcgttcaa gtggttattc gctctttgaa acgtataaag atttcttcat gatgaacctc
     4441 atcaaatggg gaacgacaag tgtaatcttc ctttttctta tagaaagaga gcctaaatat
     4501 ctttttaata tttttttctt tagcttcatt gattttattc tttcagttgt ttttattttg
     4561 tctacagaaa agaaaagtca gttattgctg atgaatggag gttaacaatt ggagccgttg
     4621 atagtgataa aaggtgcgaa aaaaatcatt cgaaacaaaa cgattcttca tttggatcgt
     4681 ttcactgcgg atgctgggga tcttgtgact gttgttggag aaagtggagc aggtaaaagt
     4741 acgttattga atatattagg attgaatgat cagttcagtt caggacaatt ctatttgttc
     4801 gatcaagaga cgaaacaatt aactgggaga gagaagcgct tgataaaaag aaataccata
     4861 tcttttcttt ttcaagattt tgggttgatt gaatatgaaa caatcgattt taatttagat
     4921 attggattga agttttcgaa tttaaatgtt tcagataagc gaacagcaaa gtatgaggca
     4981 ttgaaaaaag taaatataaa caagaagcta agtacgccta tctacacatt atctggtggt
     5041 gagaagcaaa gaatcgcctg tgcgagagtc ttattgaaga atagcaaaat cattttagcc
     5101 gatgaaccga ctgggtcatt ggataaaaga aataaggata aagtagttga aatcttatcg
     5161 caacaagctt cccaaggtac gttagttatc gtagtcacgc atgatccata tctgaccaaa
     5221 ataggaaaca agaagatcat gctttaaaaa tcaaaaagga aaggatgtcg ttttatgaaa
     5281 aaagcagtta ttatttgtgc actagggata ggttttgttg gaggaaacgc tatgtcatct
     5341 gaagtggaag caaattcagt tcacggtgaa atctattaca aatctttaaa gccaggagag
     5401 attcattttg atgctaaagg taatattatt aagtcaaatt taccagtaga ccgggaaatt
     5461 tctagaacag caattaatca tagctctgga agatgggttt attatagtga tacaacttgt
     5521 acgagaaaaa caggtcattc taaccactat agtcgtgtct ataatcgtca cggtgcaaaa
     5581 gcgaaagttg gaaactctat tcgatcagca aatgcaacaa aaggagtatg ggcctatgca
     5641 agtgcgacag gaaatagaac tgacacattc tcatgttggt acaatccaac cggttggtat
     5701 taagtatcca aaagtttata taaaatagaa aaaacatata atgaaacagc gttaaaagtt
     5761 atttttggta gttttaatat attcattttt agttgtataa aaatatatca taataaaaac
     5821 aacaaaaaaa tgtctggaaa atcgaacata aaagtgtctg atttgaatat cccagcagcg
     5881 gaagaagaaa aacaaaatcg ataacatttg ttctaagaat tagttacgtt aaatacggaa
     5941 cgcaagatag acttgaaaaa cggtaacaaa agaagtagta tattaaggtg tgatgtgcta
     6001 cttgacattt attctgctga gcgaactttc tggattgtgt agccttatat ctattcagga
     6061 cgtatcgagc agattagtaa aggtgttctt gatgattttc atcttgaaca cctttgtttt
     6121 tagcaattcg caaaaaacga aaagtaggag aattgataaa tgaaaaaaag atttttagca
     6181 cttgccatcg tactaggcac tggcttattg tcaggctgta cgaatgccgg tgaaaaaaca
     6241 gccgttagtt ataaaggcgg tacaatcagc gaacaagaag taatggatag cttgaaaaaa
     6301 atgcaaggag cagattccgc tgtgcaacag ttgatcgttt atcaagtatt tgaagataaa
     6361 tatggagatg acgtatcaac caaagaaatc gattctcaat atgatcaaac aaagaaacaa
     6421 ttgggtgatt catttgattc acaattgaaa tctgcaggtt acacagaaca aacattcaaa
     6481 gacagtatca aacaaagtct agcctttcaa gaaggtctaa agaaacatat caaattgacc
     6541 gatgaagatt taaaaacggc ttgggaatct ttccatccag aagtggaagc tcaaattatc
     6601 caagtagcaa gtgaagatga tgcaaaagat gtgaagaaag ctgctgataa gggagacgat
     6661 ttctctaaat tagccaaaga caagtcaact gatactacga caaaagaaga tggcggaaaa
     6721 gtgaaatttg attccactac tacaacggtt ccggcagaag tcaaagaagc agcctttaag
     6781 ttgaaagatg gtcaagtttc tgatgtgatc acttctacca atgcttcaac ttatactacg
     6841 gaatattatg tggttaaaat ggtgaaaaac caaaacaaag gcaacgacat ggataaatac
     6901 aaaaaagaat tgaaagaaat tgcaacagac acaaaattaa gtgacagtac cttccagaat
     6961 aaagtaattg gcgaagtatt gaaagacgct aatgtaaaaa tcaaagacaa agactttgaa
     7021 aatgttttga gtacattcac ttcagattct agtacagcaa g
//
LOCUS       DAZUKZ010000116         7025 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000116 DAZUKZ010000000
VERSION     DAZUKZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.120"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..546
                     /locus_tag="ACG8EF_002369"
     CDS             <1..546
                     /locus_tag="ACG8EF_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4947651.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
     gene            complement(716..1678)
                     /locus_tag="ACG8EF_002370"
     CDS             complement(716..1678)
                     /locus_tag="ACG8EF_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4947652.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1680..3119)
                     /locus_tag="ACG8EF_002371"
     CDS             complement(1680..3119)
                     /locus_tag="ACG8EF_002371"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFS4947653.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            3304..5250
                     /locus_tag="ACG8EF_002372"
     CDS             3304..5250
                     /locus_tag="ACG8EF_002372"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFS4947654.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5514..6386
                     /gene="scrK"
                     /locus_tag="ACG8EF_002373"
     CDS             5514..6386
                     /gene="scrK"
                     /locus_tag="ACG8EF_002373"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS4947655.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            6682..>7025
                     /locus_tag="ACG8EF_002374"
     CDS             6682..>7025
                     /locus_tag="ACG8EF_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4947656.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTG"
BASE COUNT         2260 a         1226 c         1255 g         2284 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtccc gtccaatttc gtaaaacaac cgtaacagtt
      541 gtgtaaaaat atcattttag agcatgaaaa aaggagtgga tttctccact ccaaaaaagt
      601 ctaacttttg ggggtcacta caagttgaca actccttttt gtttacaaat caattggata
      661 tctacaaatt gtattttcta acgaatttta tagacattaa aaaattaatt taacgttaaa
      721 tacttcctcc agaaagtatt gagattggta aaatataatc cttatttgtc ttttcgcctt
      781 taattttttt cagaagaata tcaacaataa gacttgcaat ttcatcaatt ggttgtctta
      841 ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt accatcgtat ccaataattt
      901 ttaaatcttc tgggatatta agtttaagtt gatgagcaat tttcatagtg agaatggctg
      961 ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt tataatagtt gactttattt
     1021 ccatttcacg acggatagta gacagattat taggaatttt gaaaattttt ccatttggat
     1081 tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga atccgtgtta tcatttcctg
     1141 taatcataat ggtattttga caaccatttt tttgtaaagt cttagctgca agttttcccc
     1201 cttcaaagtt atctgaagaa acaatggaaa cattaggagc taaatttcta tcaaaagcaa
     1261 caataggtgc tctcacacgc tcataatcag caattccaag attatggctt gatgagatta
     1321 tgccatcaac ttggttggct tcaagcattt caatgtattc tctctcttta gcaggatcat
     1381 tttgactatt gcaaataatc gttttatatc catgtttaaa taactctatt tcgagatact
     1441 caatgagttc agagtaaaag atattactaa tattaggaaa aataagacca ataagctgag
     1501 gtgactttcc ttgaagtcca cgtgctaaat tatttggttt ataaccaaga gttttcatag
     1561 cttcatgaac tttttgtttt gttttttctg ataaatatcc cttattatta atgacgcgtg
     1621 aaacggttgt tgggctaact ccagctaatt cagcgacatc agttaatttt gctaccattt
     1681 taatatttta attcaaagta ttttccagta ggttcgcctg atttgatttg aataccattt
     1741 tggtgatttt cagggaatac acgactagta aacacttttt ctcctttatt aataaaaatc
     1801 tcaatgattg agttatctac aaaaatgtta gcagttgttt ctttagtatc aattgagcat
     1861 tcacgaatcg taccaaagtc agtggcatat tgaatgcctg ctttgctgcg atctaaaata
     1921 atttttcctt ctttggtatc tacaatcaag ctaagtccat ttcctttact atcagagaaa
     1981 agaagtaact ccgatttctg atttggagga aaagttagtt caagctcata agtattattt
     2041 gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta atgatgtaat agcttctact
     2101 ggatattggt aaagtttacc atcttttatt gaaagttctt ttacaagact cattgctccc
     2161 tgataatcat acttatcagt tggataatcg atatcaggta gtccaatcca actgacaatc
     2221 agagcacgac catcaggagt attgaatcct tgagtcgcat acgcttcaaa tccataatca
     2281 agattttgaa tttctgaagc gcccacaagt tttacttttt ctatgtcaag cgattgacag
     2341 atcttaaaag tattcggata gatattccca taatttagct cagatttatc caacccttga
     2401 ggacaataaa tgagaacagg ttttttgtct acaaatacta gatttggaca ctcaatcatg
     2461 tattctgaac cagtgccgcc aaaatcaagg ttgccaactt cctcccatgt ttccacgtta
     2521 ttgtcatccg ctttgtaaag tttgataaag cctgattttt ctaaattttg tgctccaaca
     2581 atcgcataca attgtccttc ataattaaac atttgtggat cacgaaaatg atcagttaca
     2641 tcagcaggtt tatgaataag aacattctca cctttcgtaa tgttgtagtc tttatccatc
     2701 catgctccga tttgaagtgt atcacgttcc caattttcat ttcgaacgtt tcctgtatag
     2761 agtaagaata gattcccctc tatttcatat gcagaaccgg aataagcgcc gtggctatct
     2821 cgttttgtgt ctggaagaag ttctgctcct gttacatgaa aatgaactaa atcatctgat
     2881 tcattgtgta cccattcttt caacccatga gcagctccaa atggccaatt ttgataaaaa
     2941 agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat cgtttaacaa tcctgtttta
     3001 ggttcgatat gatagctagc acgccaaggt gatcgtttta cattatcctt aattttatcc
     3061 atctcatctt tagtccaatc agaataagca cgatatcgta tttctcgagg taagttcata
     3121 ttttactcct tcaatttatc tttttacaac tagtatggta aacgttttcc gttgaaaaat
     3181 caatcatttc atttaaaaaa ataaactata tgacaaacgt ttgacatatt tttaagatat
     3241 gctaaaataa atttgcattt cagtgaaccg ttttcacgta aaaataaaaa aggagtgatt
     3301 cgaatgaatt acagtaaagt agcttccgac gtaatacaag cggtcggaaa agataacctt
     3361 attgcagctg cacactgcgc tacacgtctt cgtctcgtac taaaagatga cacgaaagtg
     3421 aatcaaaaag ctttagacga aaatccagat gtgaaaagca catttaaaat taatggacaa
     3481 tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt atgacgaact tatcaaaaaa
     3541 actggacttt cggaaatttc aacagatgat ttaaaacaaa ttgctagtaa agataaaaaa
     3601 ttcaacccca ttatggcatt aattaaatta ttatcagata tttttgttcc aattatacct
     3661 gcccttgttg caggtggttt attaatggca ttacaaaact ttttaacagc tgctggactt
     3721 tttggaccaa aatcaattga agaaatgtat cctgcgattg aaggtatttc ctcaatgatc
     3781 caactgatgt ccgctgcacc attcatgttc ttaccaattt tagtaggtat ttcagctgct
     3841 aaacgttttg gagcaaacca atttcttggt gctgcaattg gtatgatcat gacaacacca
     3901 gatcttggtg gctcaactga atattggaat atttttggtt atcatgtttc acaaacaaac
     3961 tatgcttatc aagttattcc agttttagca gctgtttgga tattatctat attagaaaaa
     4021 ttctttcata aaaaattgcc atcatctatt gactttacat tcacgccatt attatcagta
     4081 atgattactg gatttttgac tttcacagtt attggccctg taatgttatt gttgtctaat
     4141 ggaattacag acattattgt ttggctatat aacactactg gatttattgg aatgggtatt
     4201 tttggaggaa catattcact tatagtcatg actggtttac atcaatcatt tccagctatt
     4261 gaaactcaat tactttcagc gtggacaaat ggtatcggac atggagactt tatctttgta
     4321 gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat ttgccatttg gttcttaact
     4381 aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg tctcagcact tcttggaatt
     4441 acagagcctg ccttgttcgg agttaactta aaataccgct ttccattctt ttgtgcttta
     4501 attggttcag gaattgctac agcagtagca ggattactta aagtagttgc tgtttcactt
     4561 ggttcagcag gtttcttggg atttctttca attaatgcaa catctattcc attttacgta
     4621 ttgtgtgaat tgattagctt tgtagttgct tttgcaatta cttacttcta cggtaaaaca
     4681 aaagctgctg gtatctttaa agcagaagaa attgctgaac aaacaccagt taattcttca
     4741 aaagtagcaa caacagatcc aatatctact gatgttgaat caacacaaac aaaagtttca
     4801 tcagaaacaa ttgttagtcc catttctggt aaagtgatta gtctttcttc agtaaatgat
     4861 ccagtctttt catcagaagc tatgggaaaa gggattgcca ttaaaccaaa tggaaacacg
     4921 gtccattctc cagtagatgg tactattcaa ctcacgtttg aaactggaca tgcttatggt
     4981 attaaatcaa ataatggtgc tgaagttcta attcatattg gtattgatac tgtatcaatg
     5041 ggaggaaaag gatttacaca aaaagttact actaatcaaa aagttagaaa aggcgatatt
     5101 cttggagcat ttgaccgttc aatgattgag aaagaaggtc ttgatgatac aacaatgatt
     5161 attgtgacca atacttcgga ttattcagaa gtaaatatag tagctgaaaa tacagtcact
     5221 gagggtactg atttacttga aatcaaataa atatatttcc ctgagtaaag atggaatttt
     5281 ctttccaaaa tcaaataata tagattaatc aaatatttga gaataacatt ttatttaatc
     5341 ttttcatata tcaattgtat gaaaaattat tagaatttta aaaaatgata aaaattcaaa
     5401 aagaaaaaat agtagttatt tacttgtcat gttactaaat aaaaaaataa tttaaatatg
     5461 atatatagtc atttatttag tgatttcatt ctaaaataaa gggagaaatg tctatgctat
     5521 acggtgcaat tgaagcaggc ggcacaaaat ttgtgtgtgc cattggcgat gaagaaatga
     5581 cgatcaagga aagagtgagc tttccgacaa cgacacctga ggaaacaatg cctttagtca
     5641 ttgacttctt caaacaatac caagcagatt tagcagggat tgggattggc tcatttgggc
     5701 ctatcgatat ccatcgcgat tccgccactt atgggtacat tacctcgacc ccgaaattgg
     5761 catggcaaaa ttttgatttc atcggtacca tgaaaaaaga atttccgatc ccgatttctt
     5821 ggacaacaga tgtcaatgcg gctgcctatg gtgagtatgt cttcggcagt ggcaaaggat
     5881 tgagcagtgt cgtttactat acgatcggta cgggtgttgg cggcggtgcc ttacaagaag
     5941 ggcgctttat cgaagggttt agtcatccag aaatggggca tatgctcgtc gtccctcatc
     6001 caaaagatga ctttgctggc agctgtccat ttcatggcaa ctgtttagaa gggatggccg
     6061 ctggacccgc catcgaaaaa cgcttaggga aaaaaggaca agaggttgca gaagatgatc
     6121 cttattggga aatcgaagcc tcctatattg ctcaatgtgc ctacaataca accttgatgt
     6181 tttctccaga tgtgatcatt tttggcggtg gtgtgatgaa acaacgccat atgctgcaaa
     6241 acgtccacga cgcatttgca aaattggtca atgggtatgt cgaaacacct gagttaagca
     6301 aatatatcgt gaccccagca ttagaagaca acgctggaac gctcggttgt ttggcattgg
     6361 ctcgagaagc ggtcttacat tcataaacga aaaaaggctc ttcgacattt agtgttggta
     6421 gagaaagata caactgaaaa tttagtcata accagttagg cagagtgctt aactggtttt
     6481 tgttttactt ggaagatcaa gccttgaatg agataaattc ttgctcgaaa gttatggaaa
     6541 tttcgatagc cgtaggccac ttgtattgtg caatagtttt ttacacgccg tttgtataat
     6601 taagcgagac aaataaaaaa gccatttatg ttacactcaa atcgtaatac ccgctaaagc
     6661 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
     6721 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
     6781 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
     6841 tgctttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
     6901 gctgaggaaa aagaatacat tgcaaaaaga tcaactgaag gttggacccc agacgtcatt
     6961 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
     7021 acggg
//
LOCUS       DAZUKZ010000117         6959 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000117 DAZUKZ010000000
VERSION     DAZUKZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.121"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            195..1052
                     /locus_tag="ACG8EF_002375"
     CDS             195..1052
                     /locus_tag="ACG8EF_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS4947657.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1045..1947
                     /locus_tag="ACG8EF_002376"
     CDS             1045..1947
                     /locus_tag="ACG8EF_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS4947658.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1980..2330
                     /locus_tag="ACG8EF_002377"
     CDS             1980..2330
                     /locus_tag="ACG8EF_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4947659.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2379..3302
                     /locus_tag="ACG8EF_002378"
     CDS             2379..3302
                     /locus_tag="ACG8EF_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4947660.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3380..4273
                     /locus_tag="ACG8EF_002379"
     CDS             3380..4273
                     /locus_tag="ACG8EF_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4947661.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4311..4874
                     /gene="amaP"
                     /locus_tag="ACG8EF_002380"
     CDS             4311..4874
                     /gene="amaP"
                     /locus_tag="ACG8EF_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS4947662.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4888..5076
                     /locus_tag="ACG8EF_002381"
     CDS             4888..5076
                     /locus_tag="ACG8EF_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS4947663.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5089..5991
                     /locus_tag="ACG8EF_002382"
     CDS             5089..5991
                     /locus_tag="ACG8EF_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4947664.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            6023..6265
                     /locus_tag="ACG8EF_002383"
     CDS             6023..6265
                     /locus_tag="ACG8EF_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4947665.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6521..6892)
                     /locus_tag="ACG8EF_002384"
     CDS             complement(6521..6892)
                     /locus_tag="ACG8EF_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947666.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2533 a         1184 c         1390 g         1852 t
ORIGIN      
        1 tagtagtttt tggtcaatac ctttgaataa aaagccatga aaaacgaata gttagcgctt
       61 accgatgcgt tttgtgataa aaaaatttta aaatattcaa tgaaaacaaa gcatatattt
      121 tgctttaaaa ccaaatgagg aatattctag atgtagaaca ggttagaaaa agattagaaa
      181 aaggagtgat tttgatggag aagagatacg gcgtagttcc tgtggtaact gcttcagacg
      241 aaaattatgc tccttacttg agcgtgatga tcgcaactgc attagaaaat tgcaataaaa
      301 caagacgcat aaaattttat gtgattgacg atggtctatc agaatatagt aaagaaggat
      361 tggaagaaac ggtaaataaa tattcaagta atgcaagtat ccaattcctg acagtggaaa
      421 aagatatcta cgaagacttc ctagtcagtg accatatcac aactaccgct tacttaagaa
      481 tttctttacc aaacttatta gctaaagagg attataagaa agtcctttac ttagattcag
      541 atgtgttagt actagacgat atcgtaaaac tatatgatga gccgttaaat ggaaaaacaa
      601 tcggcgctat tatcgatcca ggccaagtga aagctttgga aagattagga atcgattccg
      661 atgacttgta cttcaattca ggagtgatgg tgattgacat tgatcaatgg aataaaaaag
      721 aaattacaga aaaaactatc cattacctca gtgaaaatgg cgatcgaatc atctatcacg
      781 accaagatgc tttgaatgct gtactttatg aagattggga acaacttcat ccaaaatgga
      841 acatgcagac ttcgctgatt tttgaacgtc atccagcacc aaatgaaaaa tatgaacgtc
      901 agtataaaga aggaaatgaa aaaccttcga tcgttcactt tacaggacat gataaacctt
      961 ggaatacgtt gaaagatcat ccgtatacca atttatactt aaaaaaatta gcacacagta
     1021 ctctaacgaa agtaggcgag atcaatgaat aaaaaagaaa tagcagtagt cgcaagttgt
     1081 aacacaaaat tcgtaccaca tttagctgca ttatttgtct cagtattaga taattgcaac
     1141 ccgtctaaat ttgttcggtt ctatgtgatt gacgatgata tcgattttga aagtaaacag
     1201 cttttacgct tctcagtgaa aaacgcacga atgaactcag atgtggaatt cttgaaaatc
     1261 aataaagaat tctttacaaa tgtggtaatc agcgatcgta ttccagaaac agcttattat
     1321 cgtattgcga ttccggaatt gttccgcggc acagaagttg aacgtatctt atatatggat
     1381 tgcgacatga tcgctttaca agatatctct aaattatggc gtcttgactt cggtgattca
     1441 atcgtagcag ctgttgaaga cgctgggttc catcaacgac tggaaaaaat gaaaatttca
     1501 gctaaatcaa tgcgttattt caactcaggt ttgatgctga tcaatgtgaa aaaatggtta
     1561 gatgaaaata ttacgcaaaa agtattagat ttcatcgaac ataatccaga aaaattacga
     1621 ttccatgacc aagatgcatt aaatgctatc ttacatgacc gttggttacc acttcatccg
     1681 cgttggaatg cacaaggcta tatcatggca aaagcaaaaa aacatccaac agcagcaggt
     1741 gaacgtgaat acgaagaaac tcgcaacaat ccttacatta tccatttttc aggacatgtg
     1801 aaaccgtgga gcaaggattt tgaagggcca acgaagaaat attacgaaaa atatgcaggg
     1861 atgacggctt tccgttgtgt agctaaattc cctaaatatc caaaatatgc aaaagttcaa
     1921 ccaaaaacag atgtagttgt taaataatag aagacaacaa ctgaagaggg ggagtaggaa
     1981 tggaaattac aatgtctaaa aatgcagtag agacgttaat tgaaaaggtt ggggaaaata
     2041 caaaaatcgc actcgcgctt atcaatgatt ccgatccatt tctaagggac aaaggtgctt
     2101 ttgcaaaagg cagctttttt caaatcattc cttttgtttc ggaatttgga gaatatgcga
     2161 ctaaaatcga gcatccatta ttggacatct atacatctaa attagagcaa aattatttcg
     2221 gtaaacgatt gaacatggac ttcaacaaac agcttgacag cttttcatta gaaaatgaaa
     2281 tcgcagtact cgattataat atcaaattaa agaactgctt cttcagctaa tagtttcgag
     2341 aaagtccaaa aaggaggtat acattgagat actactttgt ggatgaagaa aagaatgtaa
     2401 tagaaacaga tgaaaaaaaa tataattggc tcgtacttga tagtgcaaag cccgaagaga
     2461 ttgagcaagc aatcgaagaa ttcgagcttc ctgatgatat tttcgtagga accgaatatc
     2521 cggaagaagt ttctcggatg gagcgccttt atgagacgaa actagagcat ccattttctt
     2581 tggtagtgat taatctagat cagtcagata atcgtatcga aaaaaaatta acgccaatat
     2641 cttttgtact ttccgataat ctcttgatta cttgtatcga tagaaaaacc gaatttgtgg
     2701 atcggctgct gacaaagcat ggaaatcatt taaactcgtt tgaaaagatc atcacgtata
     2761 cattattaga tatttacacc cattacgtga aagaactaag agagatgaaa aagcgaattg
     2821 acgctttgga taaagcagct cgtaaaacaa cggaaaatga agaactgtat aaacaggcag
     2881 atcttgaaag agacatcgta tatatcgatc ataccttaag agatcaacgg gaaacaatgg
     2941 atcaattatg gaattctgaa actttcatca aacgtttaga cgatgaacag ctactctatg
     3001 atgtccattt gagacagcga caaacagaaa aaatgatcga gatctatcga gatttattag
     3061 agagtattgg tgatctattc aacgggatga tggacaataa tctaaatcat atcatgaaat
     3121 atctagattc agctgcatta atcatttccg ttccggcgtt gttcgcaggg atttggggaa
     3181 tgaatacagg aggattaccg ggtaaaagtt ctgctctagg ctttatactc gtagtctttg
     3241 gctctttgat agcagcagta gttttaggtt accatttata ccggaaagat tatacaaatt
     3301 aaagaatcta tttaaaacag gtatcaaaca aagaaatttg ttttggcaat acaatttaaa
     3361 aactaggagg aatacagcaa tgacagaacc aaaattaatc gacccgcgaa acttgtacca
     3421 tacggagaaa tttcctaaac aagatcagga tacaccagct ttacagcaga atatgattcc
     3481 tgttcctgat tgtggagaag aatcctatga aggcaataat caactagaaa accgccgtgt
     3541 actaatcact ggtggagatt cagggatcgg tcgtgcagca gcgattgcat ttgctagaga
     3601 aggggcagat atcgctcttc aattcttccc aggagaagaa aaagatgcag aggaagtagc
     3661 aaaatacgtg aaagatgcag gacgtaagat tgtcttacta ccttttgatc ttcgtgacga
     3721 acaagcacct aaagaaatca tagataaagc cgtgagtgaa cttggcgggc tagatacgat
     3781 ggtactaaac gcaggacagc aaatttcttg tcagtctatt gctgagttac cacttgagca
     3841 agtcaaagac acatttatga taaacatcat cgctatgttt gctttagtga aagaagctgt
     3901 tccgcatatc ccagcaggag gagcaatcgt aacaacaact tctgttcaag cattcaatcc
     3961 aagcgaacat ttattagatt acgcagcaac aaaagcttca atcgctaact ttacgatcgg
     4021 tttagcaaaa cagcttgcac ctaaaggaat ccgtgtaaat ggtgtagcac ctggtccaat
     4081 ctggacacca ttacaactag acgaaggaca accttctgat tcgatccctg aatttggtca
     4141 gcaaagttta ctagaacgtg caggtcagcc aggagaatta gcaccagttt atgttttctt
     4201 agcatcgaat aaagcaagtt atgttacagc ccaaatttac ggagtaactg gtggagaagc
     4261 gatcaactta taagaagttg atcgtttcac ccctatgagg aggtgcgaaa atgaataaag
     4321 gtgtaaaaac gatcggagtg atcggaacat tacttttgct atctgtgctt ttgtttgcga
     4381 tactattaag tgcaacctat ccattgcctt ttcagctaga gaagtttcga ttcttttcca
     4441 tcactaattc atacgtgcaa caatatgtat tttggcttgc cgttgcattt gctattttga
     4501 caattatcac gttacttgtt ttcctgtttt atccaaaaac aatcggaaac tttctactga
     4561 aacatggtga tggggagttg acgttggata aaaaagcaat tgaaggaatg gttcgttctc
     4621 acttgcatga agatgaattc atccattcac caaaagtaaa tgtcaaagca acgaaaaaca
     4681 agatcgccgt caatgtaaaa ggggagttga aacgaacttc agcactcatt ggtaaaacag
     4741 gaacattgat gaaagaaatc gaaaatgaag tacaacacat attgggaaca aaagagccag
     4801 tgaaagtatc ggtgaaatac acaggctttg aagaaccagc taaacgaagc aatgaccgca
     4861 aacgagtaga ataggggggg accaaaaatg aaagaactat tttcgacata caagctccct
     4921 atcatctttg gggtacttgg attgatatta gccgttttat ggatcaccgt gggctttttc
     4981 aagaccttat tgatcttgat catggcagcg atcggcgtcg ctatcggttt ttatctgaaa
     5041 caaacgaaaa tcttagatca ctattttaaa tcttaggagg aaacatccat gaataacaat
     5101 aacaaaaata ataacaataa aatgaacaac caaaaaccag tgtctgcaca tccagaccca
     5161 acgacaccaa accaatcaaa tggtgtgaac gatgaaaaga acaaaacagc accagtatct
     5221 gcacatccag atccagtagt ccctaacgtt caacaaggag tagaagagaa gaaacatgat
     5281 gcagaaaagc atgcacctca tcatccagga actccagaat ctaaagttcc agaacaacat
     5341 gatgcaatcg atccttctaa acctgtggaa gagcaaaaac atccttctga ccatgcgaaa
     5401 gcacaagaaa aacttagttc tgaccacaca aacggacaag gaaaacttgg ttctgatcat
     5461 acaaataacc aaggaaagca tgattctgaa caggaagatt caatgcgcgg tgagctgaat
     5521 tatgaagata aagttgtgca aaaaatcatc ggaatcgcaa ttgaacaagt agacggttta
     5581 ttatctgcaa acggtggttt cttctctaat gtggcaggaa aacttgtgaa tacagataat
     5641 gtaacggctg ggatcgaaac agaagtcggt aaaaaacaag tagcagttga tatggatgtc
     5701 attgtggaat atggcaaaga tatcgaaaaa atctttgaag aaatgcaaga agtaatcggc
     5761 aaagaagtaa aaaatatgac acatttagaa gtgatcgaag tcaacgctaa cgtagtggac
     5821 atcaaaacaa aagaagaatt tgaaaaagat caagaaacgg tgcaagacaa agtaactgac
     5881 gcagcgaaaa aaaccggtga atttgcttcg aatcaaacag ataaagtaaa atcagcagct
     5941 ggaagcggcg cacaaaaagt aaaagaaaat acagaaccac gggttcagta ataaggaagt
     6001 aaaacattag gaggaacgaa acatgggatt tatttggtca cttatcgtcg gaggaattat
     6061 cggagcaatt gcaggagcaa tcacgaataa aggttcttcg atggggatta ttgcaaatgt
     6121 cgtagcaggt ttagttggat cagcacttgg tcaagcatta ttaggttctt ggggaccaag
     6181 tattgcagga atggcaatcg tgccatcatt gattggtgcc atcattttag tactaatcgt
     6241 atcattcttt gctcgaaaac tgtaaaaaga cttttaaaaa gaggtcgtga aaaagctgtt
     6301 cttcatcaag taataagaac agccaaacaa aaatagcttc tcatattttt catttggttg
     6361 ggcatattat cgaagatgca agcttttgaa cactgtttat acggaataag aggctgtgac
     6421 aagacttttg tcacagcctc ttatttatgc gggcaaattc ccaattagat ttttaaaagt
     6481 ttttccttaa atgtgtcttt attgatacag agaagcttct ttaataaaac aatgcctccc
     6541 taaattttct tgagatagga aaggtgaaca tacaaaataa tacgaccact ttgaaaaccc
     6601 agatgatcca caaatggatc gtatgatact gatgaagtaa aaagcaatgg atcagaaact
     6661 caaagagatt gaaaaagaca aggaagaaat aatatctctt ttttactctt gtctgtttta
     6721 gccaataata gctcgtaaag taatctagac aaacaaatag aagtaaacaa agtttcgtaa
     6781 gagcagcaat cgtatcatgt tgctctgcta gacgattgac gagcaggaaa aatgttggta
     6841 atggaatata gatcacgagt aatgaaagaa aaaagtaaac aataaatggc atactattcc
     6901 tcctaattgt gtgagttcgc cctcatttta aaataacgat atttgtttgt gaagctttt
//
LOCUS       DAZUKZ010000118         6817 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000118 DAZUKZ010000000
VERSION     DAZUKZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.122"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG8EF_002385"
     CDS             complement(<1..182)
                     /locus_tag="ACG8EF_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4947667.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG8EF_002386"
     CDS             517..951
                     /locus_tag="ACG8EF_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4947668.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG8EF_002387"
     CDS             974..1294
                     /locus_tag="ACG8EF_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947669.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG8EF_002388"
     CDS             1435..1626
                     /locus_tag="ACG8EF_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947670.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG8EF_002389"
     CDS             complement(1697..1954)
                     /locus_tag="ACG8EF_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFS4947671.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG8EF_002390"
     CDS             2112..2375
                     /locus_tag="ACG8EF_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947672.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG8EF_002391"
     CDS             2399..2587
                     /locus_tag="ACG8EF_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947673.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACG8EF_002392"
     CDS             complement(2670..2855)
                     /locus_tag="ACG8EF_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947674.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACG8EF_002393"
     CDS             3070..3441
                     /locus_tag="ACG8EF_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4947675.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACG8EF_002394"
     tRNA            complement(3591..3663)
                     /locus_tag="ACG8EF_002394"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACG8EF_002395"
     CDS             complement(3709..4137)
                     /locus_tag="ACG8EF_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947676.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACG8EF_002396"
     CDS             complement(4197..4955)
                     /locus_tag="ACG8EF_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS4947677.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACG8EF_002397"
     CDS             5250..5447
                     /locus_tag="ACG8EF_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947678.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACG8EF_002398"
     CDS             5553..6035
                     /locus_tag="ACG8EF_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947679.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACG8EF_002399"
     CDS             complement(6093..6689)
                     /locus_tag="ACG8EF_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4947680.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         2312 a         1054 c         1341 g         2110 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc cccagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattt
//
LOCUS       DAZUKZ010000119         6783 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000119 DAZUKZ010000000
VERSION     DAZUKZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.123"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(23..856)
                     /locus_tag="ACG8EF_002400"
     CDS             complement(23..856)
                     /locus_tag="ACG8EF_002400"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFS4947681.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(1048..1752)
                     /locus_tag="ACG8EF_002401"
     CDS             complement(1048..1752)
                     /locus_tag="ACG8EF_002401"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4947682.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(1775..2401)
                     /locus_tag="ACG8EF_002402"
     CDS             complement(1775..2401)
                     /locus_tag="ACG8EF_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4947683.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            2727..5102
                     /locus_tag="ACG8EF_002403"
     CDS             2727..5102
                     /locus_tag="ACG8EF_002403"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFS4947684.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(5126..5617)
                     /locus_tag="ACG8EF_002404"
     CDS             complement(5126..5617)
                     /locus_tag="ACG8EF_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFS4947685.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            5804..6724
                     /locus_tag="ACG8EF_002405"
     CDS             5804..6724
                     /locus_tag="ACG8EF_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4947686.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
BASE COUNT         2183 a         1238 c         1313 g         2049 t
ORIGIN      
        1 aagagaagtg ttctggtttt ttctacattg caataaacca ataaccaatc aaaatgatac
       61 caaggataat acgataccag ccaaaagcgg taaagtcgtt tttcttcaaa tagcttagta
      121 agaatttgat agctaagacg gaaaccacaa aagatacgaa tgttcctaca agaagaacaa
      181 cagtttcttc cattttgaat gaattccctt cagctaaata tttgacgatc ttcaagccgc
      241 ttgcgccaaa catgacagga atccctaaga aaaatgaaaa ctctgtagcg acaaaacgcg
      301 aagcaccaat caagatagat cctaggatcg ttgcgccaga acgggatgtt ccgggaacaa
      361 gtgccaagac ttggaataac ccaacaagaa gagctgcttg gtacgtaaag tcattcagag
      421 aagtccaacg aggtgttttg tttttgttgc gtttttcgac aataataaag gcaataccgt
      481 aaacgatcaa aacaatggca actggtaaga acttgtggaa atttgcttct aaccaatcat
      541 ccaaaggcaa accaatgatg atggctggta tgcaagcaac gatcacttta aaccaaagtg
      601 tccacgtatc ttttttttct accgttgatt tacttggaga aaatgggttt aacttgtgaa
      661 aataaagaac gacgacagcc aagatcgccc ctaattggat aacgacattg aacatcgaca
      721 taaattgtga actggcatcc agtttgatga attcatctgc taggatcaaa tgaccagtac
      781 tactgatggg cagccattcc gttattcctt caataatacc gagaatcact gctttgataa
      841 tatttgcaaa aaacataaat tccttccttc ccaattaaga tacatcttat agtataccgt
      901 tattttttga gaaacccaat ctgtttttta atattaagaa aaacttgaga aataaagcag
      961 ataagtacac gaagagaaaa aagaagacaa agccctcgtt cagactttgc cttttatgat
     1021 tctctggtaa aaattaaaat atccccatta tagaaacgag tcgcgactat gtgttttccc
     1081 gttatcgata taagacgtat cattgatgcc gtggatagaa aatcgaccgt ttgcataagt
     1141 gacctttgtt acgctgccat ttaaaagccc agcacgaaca ggctgtttag gatcgatcag
     1201 attaagcaaa aaggcgatcg tcaaaccatg agatacgact aaaacatttc caccaccatt
     1261 tttttcgcag tgatgggcga tatcttcaaa tcctgtccat acacggtctc gcaatgtttc
     1321 ataagtttcc gcccacccag cggtatctaa gttgtataca gcattagcaa tttgttcaaa
     1381 agaaacttct gtggtaaaca tttcatcata tgttttaaaa ttcaatacac gagggatcac
     1441 accccacatt tccatgtcat atccaccttc aagagacccg aaacaccatt ctctaatcct
     1501 tttttcaata aaataaggaa tttcttttcc cttagaatgt tctcctagaa taatacgggc
     1561 agtttcgatt gcacgaccac tgtcactaga ataagcaaat gaaaagtcga cattttttaa
     1621 cccgcgacct aaataatgaa tgccttcttg tccttgcttt gtgaggggtg tatcacacca
     1681 gccttgtact cgttcaattg tattgaacat cgtttttccg tgtcgtacga tataaagtgt
     1741 agtttctgtc attttattct cctcctaagt cgttttaacg attgaaaaaa ggatttgtcc
     1801 ttttttcctg ttcaatcgtc gttgcatcgc catgtcctgg atagactgca aattcatcag
     1861 gtaatacaaa cagctgggtc cgaatactat gaagtaattg ttctaaattc cctgtataca
     1921 gatcggttct accgatactt cctctaaaca gggcgtcgcc agaaacgaca aaatcatcaa
     1981 aaataaagct gacgcttcct atagaatgcc ctggggtagg gacgacgcga aaagacatgt
     2041 ttcctaaagt ataagttttc atttcaaatt catattctgc aggcgagaca ataacatcag
     2101 caatatcatc atgtctaccg agtccagaaa gattcaaaat cggatcacct agccattttt
     2161 gctctagtgg gctgacataa actgggattt gataatacgc gcgtaaaggt tccactgcac
     2221 cgatatgatc gtagtgagta tgggtaagca aaatcgctac cggctgttgg tttgtttttt
     2281 tgatttcttg tttgattcgt tccgcatctt ctccagggtc aatgatcaaa agtgcttctt
     2341 cgttgtagac caaataacag ttttcttcaa tcattcctgt ttttaatctt tcgatatgca
     2401 tgtgcattcc tccttgatag ccaaaacttc tttcatccat tagtataacg cattcattgc
     2461 taggaaacaa aaacaggtac ttatttctct ttacttatct gaattatcag actatatgaa
     2521 aagtatttgg atattctaaa gagaaggaaa gtaagttttc atagcatttt aatatgtcct
     2581 tgttacatta gattcatcca aaaatccaaa ttctatatcc tatatccctg cctaatcagc
     2641 agggtgtttt tttatgtttg gaaagagagc tctttttgct aaactaggtt ttttcaggta
     2701 atcttagaga gaaagaggtg gtggaaatga tcaaacagtc aaaaatatct gtgcggcact
     2761 tagtagaatt tattttgcga cgtggaagta tcgacaatcg taaaaaaagt aaccatactg
     2821 cattagaagg agcgagaatc caccgcaagc tccaaaaaga tgcgggagaa aactatgaaa
     2881 aagaagtctt tttaaaaaca acagttcaat tagatgaata ttacctgatc gttgaaggcc
     2941 gtgcagacgg tattttcaaa agagacggta tttattatat agatgaaatc aaaacctccg
     3001 aaccacgatt tgaagattta gaaccagaac aagtggaact tttctttcat caagcaaggg
     3061 tgtatgccta tatctatagt caagaacatg atttacaaga aataaatctg caattgactt
     3121 acttccagac ggcagaagaa gtgatcacga gaaaggttga acatcagaca agagagcagt
     3181 tagaaacttt ttttaaaaag ctgactgaag attataaaga atggcttgtt tttcaagaaa
     3241 actggcgcac tgtacgcaat gcctctttga tggctttgaa attcccccat gatgagtata
     3301 ggaaaggtca gcgtgaacta gcggtagctg cttataaaac gatccgtacg aaacagaaat
     3361 tgtttgtaga agcacctacc ggaacaggaa aaacaatctc tacgttattc cccgctttga
     3421 aagctgtagg ggaagaagag ggagaaaaga tcttttatct aacggctaaa acaataacta
     3481 gacaagtagc tgaagatgct atgacagcat taaaagatac aggagctgaa gtaaaaagtg
     3541 tcactttgac ggcgaaagac aagatctgct ttttagatga gacgacctgt aatccagatc
     3601 agtgcccata tgccaatggt tattacaatc ggattaatga aggtttatgg gatcttttga
     3661 atcatgagaa tcagatcact cgtgaagtca tcgaaactta tgcaagaaag catacacttt
     3721 gtcctttcga actatcatta gatgtcagta tctggtgtga cgtaatcatt ggggattaca
     3781 attatttatt tgatccaact gtttatttaa gacgtttttt tgaagatgaa aaaaacgagg
     3841 actatctgtt tttgattgac gaagcacata atttggtgaa tcgttcccga gagatgtatt
     3901 cggcagaact atcttatgaa aaaacgaaac gaagtaagga agctatacca aaggaattca
     3961 aaaaacttca tcgccgtttc aacaaactat taaaagaatt tgattcgatc cgagaaatag
     4021 caaaagaaga tcattgggat tatcatcatc aaaaagcacc ggctgaatct ttagtaaagg
     4081 caggttatca attaagcgaa aaaattaaag aatggctggc agaatttcct gagcacccat
     4141 cacaagaaca attgcttcct tattattttg atttgcttca ttttttgaag gtcagtgaat
     4201 tttatgatga tcactacgaa acaacagtcg aaaaaacgta tcgcgatctg atagtaaaag
     4261 agttttgtat tgatccaagc ttgtttcttg agcagtcgtt ggataaagga aaaagcagta
     4321 ttcttttttc tgctagtttt tcaccactct cttattatca ggaaacactg gggggacaaa
     4381 aaagtctggc ttacaaactg ccgagtcctt tcccagaagg aaaccagcaa gttttcatcg
     4441 caaattacct agagacgact tatcgaaaaa gggaacacag tctggcaaaa ttagtagaga
     4501 cgattcatgc ttttgctgat ggaaaggtcg ggaattattt cgttttcttt ccttcttatc
     4561 aatatttaga tcttgcggtg gaagcattca gacagcaata tcccgacagc aatgtgctaa
     4621 tccaagaaac ggacatgaat gaggaagaac gcgaacgatt tttaacaaaa tttcaaacaa
     4681 atcccgctga aacattgatt ggattttgcg tacttggcgg gattttttca gaaggaatcg
     4741 atttgagagg ggaccgattg atcggcagta tgatcgtagg tgtaggtttg ccgcagatga
     4801 atcatgagca ggaactgatc aaatcatatt atgatgagaa ggaacacctt ggatttgctt
     4861 acgcttatca gcttcctgga atgaacaaag tcttgcaagc tggtggtcga gtgattcgtg
     4921 gcatggcgga tcaaggaatc gttgtattgg cagatcaacg attttctagt ttttcttatc
     4981 gacgtttatt cccacaacat tggcaaacag caagagaagt acgaagtgtt ggggaattgg
     5041 aagaaggaat acagcgtttt tggttatcaa aagaagcagt aaatcaaaaa aacgaggaat
     5101 gacagacgtc agcctcgttt tttgattaac gatagttagt gaattgcaac tcgattggta
     5161 agtcaagttt acgtaataaa gcaatcacag cttgtagatc atcacggttc ttacctgtca
     5221 ctctaatctg atcttcttgg atttgtgctt ttactttcag tttttcattt ttgatagctg
     5281 tagtgatttt cttcgcgttt tcacgatcga ttccgttgac caaatcggca gtttgtcgag
     5341 ctttgccacc tagagcgtgt tctgattcgg aaaaatggat atttttgatt ggcacatttc
     5401 ttttgatcag cttaccaaat aatacatctt tgatctgttc gattttgaaa tcatcatcgc
     5461 tcaatacaac tagattgctg ttctctaatt tgatttctac tgtagaatcc ttgaagtcaa
     5521 agcgattgga caattctttt gttgtttgct gaatcgcatt ttttacttct tctgtattga
     5581 cttctgatac aatgtcgaag ctagcatctt ttgccatagg aaccctcctg atatacatat
     5641 aaatatttac catacatcta tcttatcata ggattagggc agattcttac ctgtttgcta
     5701 aaattcttct catttgagtt cgaagtattt tattttaata gataaactgt tattcttaca
     5761 tttggaatac caaagtaggg gagaggtgct tgtgagtaaa attatgactg cgtatcataa
     5821 aaatgaaaga ttaaagaaaa tagtggttat tttgataacc ggattgaccg ctgcggtcgc
     5881 tttaaattat tttttgattc cagccaatgt cttttctgca gggatgaatg ggatcgctca
     5941 aatcatttcc tcgcttttat atgaatggtt tcatatcaag atagacacag gaacatttat
     6001 ttttcttttg aatatccctg tgtttgtatt agggtttctc aaagtaggaa aacgagcaac
     6061 gatccttagt tttgtgaatg ttgtatgtgt atcggtgatg acgatgctgc ttccagtcgg
     6121 acagatcact gataatattt tgatgaatgc cctagtaggc ggtgttttat taggtcttgg
     6181 cgtaggatta tctttaaaaa tgggctttac aactggtggg atggatatca tctctcttgt
     6241 actttcacaa acaactggaa aaacagtcgg aaactacatg ctgattctaa atggtatcat
     6301 tgtagtcatc gcaggcttct tctttaactg ggaaagtgcc ttgtatacga ttatttccat
     6361 ttatgcaatg actcaagtaa ttgatgccat ccacacgagt catcaaaaag taacagcaat
     6421 gatcgttacc gtcaagccag aagaggtaac agaagaacta gcaaaacgga tggtacgtgg
     6481 aatgacatta ctgccgtctg tcggtggcta ttcaaaaaaa caagggaaca tgatcatgat
     6541 ggtcatcacc cgctacgaaa tgtatgatct tgatcagatc gttcatgaaa tcgacgacga
     6601 tgcatttatc aatattttac caactcattc agtatttggc cggtttgcaa atgaaaacga
     6661 acaaaaaatg tatcgaacaa ctggcgtatt gccagaaata aaaaatcgtt cacaaaaaaa
     6721 atgaggaatc aggtttattt tagaataaat ttaatctatt acatctaata agaacatttt
     6781 tat
//
LOCUS       DAZUKZ010000120         6740 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000120 DAZUKZ010000000
VERSION     DAZUKZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.124"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..517
                     /locus_tag="ACG8EF_002406"
     CDS             2..517
                     /locus_tag="ACG8EF_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947687.1"
                     /translation="MKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKAEKFCLL
                     LNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPP
                     KSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNK
                     NNNFYFYRNKL"
     gene            complement(643..1320)
                     /gene="nth"
                     /locus_tag="ACG8EF_002407"
     CDS             complement(643..1320)
                     /gene="nth"
                     /locus_tag="ACG8EF_002407"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFS4947688.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1327..2025)
                     /locus_tag="ACG8EF_002408"
     CDS             complement(1327..2025)
                     /locus_tag="ACG8EF_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS4947689.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2293..3477
                     /locus_tag="ACG8EF_002409"
     CDS             2293..3477
                     /locus_tag="ACG8EF_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFS4947690.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3483..3926)
                     /locus_tag="ACG8EF_002410"
     CDS             complement(3483..3926)
                     /locus_tag="ACG8EF_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS4947691.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4152..4439)
                     /locus_tag="ACG8EF_002411"
     CDS             complement(4152..4439)
                     /locus_tag="ACG8EF_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS4947692.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4610..5962)
                     /locus_tag="ACG8EF_002412"
     CDS             complement(4610..5962)
                     /locus_tag="ACG8EF_002412"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFS4947693.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6235..6681)
                     /locus_tag="ACG8EF_002413"
     CDS             complement(6235..6681)
                     /locus_tag="ACG8EF_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4947694.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT         1827 a         1327 c         1102 g         2484 t
ORIGIN      
        1 tttgaaagaa ggcttcattt tgagttatct gatagtatgc ttaattatca taatttgtct
       61 gcaccatctt tttttatcga aaacaatacc tcgttattcc aagaataaaa aagctgaaaa
      121 attttgttta ttattaaata aatttacttt aattgctcct atccttgcat ttattatttt
      181 ttctgtactt ttaagcacta ctcttaaagg aaaatttatg gaaagaagct ctcatgcaat
      241 gattctcact tttttatggt tgttgttcac cagaatctat atttttctga tgagcctgaa
      301 accgcccaaa tccattagtt tatgtttagt tataaatggt atttttttat tatcacttat
      361 catttttatt acacctttag atagatacgt cacttattta tataatcctt tggaatactg
      421 gacttatttt ataggtatat tggaaggcat tattttctac atcgggtatt ttcccaataa
      481 aaataacaac ttttattttt atcgcaataa attgtaataa gttaccatcc tagctactga
      541 gtatgtacat ttactttttc atgcaaaaaa gataaacgaa acagcttttc tccataataa
      601 caagataatt catacattta tttgcaatta aagaaaccac gtctacaggc gtggtttctt
      661 ttttagcgcc ttttctttta gacgcatccg atttttccca tcttgacata ttgatagcaa
      721 aggacatacc tcgcacttgg gatttcttgc cgtgcaatga tatctaccaa aaaaaatcag
      781 tgtatgatgt gtctttaccc ataattcttg tggtactttc cgcatcagtg tttcttctac
      841 ttccatgacc gtagcatcta gtttgcagat cctcaggcgt tttgagacac gttccacgtg
      901 ggtatctact gcgattgctg gtatcccgaa tgcatccccc aatacgacat ttgctgtctt
      961 tctgcctaca ccaggaagag acatcaattc ttctcttgac gttggcactt gcccatcgaa
     1021 acgttcaatc agctgctgtg cacacgcttt gatgtttttt gctttatttc gatacaaacc
     1081 aatcgttttt attttaagaa tgatttcatc gattgaagct tctgctaacg catcgggtgt
     1141 cggaaaagag gcaaacaaat ctggcgttgc tttattcact gatacatctg tcgcctgggc
     1201 acttaagatc actgcaatca ataactcaaa tggattattg tgcttgagtt ctccatgtgc
     1261 ttcgggaaac attccataca tcgtttctag agcttccatt gttttttgtt tgcttaacat
     1321 attccgctac tcctccaacc aatttttcag tgagactttc ggcaattctt cttgttgagc
     1381 tgactgttct acttctcttt gaagaagctg ctgttttctt cgtttttggt cttcttccac
     1441 ttgttgtttt gtacgtagat tttttctttc ccacgatagc aaaatgcggt caacgtaatt
     1501 aaaactataa gcctgattta atacggcttc ccgcagtgct agcttcaaaa tttccggctg
     1561 ataatggtct tcttctagcc attgtccgat ccgctggaac tcgatcgaag aaagcgggcg
     1621 tccaaattct tgttcaaata attgatacac tgactggatt tctttttctt gtgttttttc
     1681 ttcgtatttt ttttcttgtg tttttaaata ctcacctaat agatcataga tggggtaaag
     1741 attatatcga tctgcttttt taccgttgac aatcgtcgtt tctatcttga taaatccatt
     1801 tgttacgaga cgatccagta tttgaaaaat ctgatcttgt ttgattccca tatccatagc
     1861 aatcatctgc aaatcaggga aagtatctcc ttcaagctgt gccatatgca actgtagaac
     1921 aaataaaaat tcttcagatt gtagaccgat cctatgataa tttttaagta aaagattaga
     1981 tatgactgtt tgtccagcgc ttaagtaatt ttctaaattc aacatgattt ctcctccgct
     2041 caatcacagt atacgtaaaa aaaacatggg aagcaagaac aggtttcttt tctatttact
     2101 tcactgatcg gtcagattta caatgtcgtc tacttgattt ttgtttgttc atgtttttga
     2161 tgcgtatctt cacttttcgg aataaataaa ttaagtagat ttcgctgttc cttagaatgt
     2221 tgacaaggaa acaaaattac tatacactat ttttgtttcc aagttaacat tattgcataa
     2281 aggagttttt acatgtacag tttcacaaat aaacattcta aagtttttgg atacgtctct
     2341 gtcttattca ctggattagg atttactatc gtcagtcctg tacttccgtt tttaacgctg
     2401 ccttattctc actccataca tcagcaagct ttctatatca ctttgctcat gtctgtttat
     2461 gctctagctg ccttcttatc tgcccccatt ctcggtagct taagtgatca ttttgggcgt
     2521 catccgatcc ttattatcag tttattagga tcaagtatcg gatatttgct ttttggttta
     2581 ggaaattcga tatggatgtt gttccttggt cgtatcattg aaggattgac ggcaggagaa
     2641 atctccacct tatatgctta ctttgccgac atcacggaac ctaatgaaag aaccaaagtt
     2701 tttggatgga tgggtgcttt agtcggtata ggaacaacgc tgggtcctat tatagggggt
     2761 ctattagctg agttggggaa ttctgttcct atatttattg gagcactctt tactttctta
     2821 aatgccgttt atggctacac atttatgcta gaaagccttc caataaaaaa acgttcagta
     2881 gatttaagtt tctcccatgt aagacctttt caccaattac aacaattatt caagatttca
     2941 tccgttattc ctttattgac agcaggtttt gctgtttggt tggcagctgg ctctcttcaa
     3001 tctatctttt ctcaattttc gattgacgcc tttcagtgga aggctgggtt ggttggttta
     3061 agcttctctt tgattggaat cttggatact gtctcgcaat tgttcattat gcctcgattg
     3121 ctcaaaaaat tttcagaaca gcagatcaca agaataggga tgttcagtga aatcctcgcc
     3181 tatctattca tcacattatc tggaattctt ctactgccga ttttgttttt atttgggatt
     3241 atctgttatg gtttcggtga ttctattttt actcctgtat tcaatggcca actatccaat
     3301 tctgtctcag ataaccagca aggtcttgta atgggcggta cccaaagtat acaatctcta
     3361 tcgcgcgtca tcggcccgct aatcgcagga cagctttatg ctgcagcacc atttcttccg
     3421 acggtattcg gatttttgct tatgattttt gcctattatc actacaataa aaaatagtag
     3481 cttcatttgc tacttcgtat aaattctctg tatcgaccga taaaacgaaa tactgtgtca
     3541 aattcttctt ttgacatatt ttcaaataca gtagcatctc gctgtaccca tttttgatgg
     3601 agttgctcgt gtctattcct tattttttgt ccgattggcg tcaattgaaa atagatttct
     3661 tttttattct cttcgctttg ataagtgttt atgatccctc tagctatcag tttttttgtt
     3721 agcttactga tggctcctct agtcatgtaa ctagcattcg ctaattttgt cacgttagga
     3781 tcagggattt tcccgatatg ttcaatcact tctagttcat ttagagtaaa tcctgcaaat
     3841 tctttttcca tttctggttt attacgatga ataatctcat tagctaaatc cagatattct
     3901 tctattagca gttgtctttt atccatttct acaccttctt tttctttcat tatattgttt
     3961 ttttgtttcc tattcaacaa agataaaaat attttgtagg aaaacccaat ctgaaaccaa
     4021 gaaaaaaagg ggttgacaaa gcttgtcata accccttttt ctgattaaaa gatagtaaag
     4081 agacagtcat cggtaattgt gatgattttt tgtcatcact taaggatgag cggccttttc
     4141 aaaaactgtc tttaaagatc atcatcagtt tctagcacat atggctgacc ttcatatgtg
     4201 tactctgtat tcaatgtgcc gtcattttca tctgagtaga tgcgtctact gaaaggaaaa
     4261 cgatgagaaa cattatcttt ggcgtcattt gctggcatcg tgtaatattc ctcttgcgtt
     4321 tcattcaact gagagagtac ttgatctgcc aactcatcaa tattgatcgt taatttcttc
     4381 gaagcacttt tataatcttc taaaaatgca tcttgttgga caaaatattc tttattcatc
     4441 ttctttctca cctttctttt caaaatcaaa taaagctgct tgttctttta tgtaacagct
     4501 ttatctttgc tgcttattgt gacaaaaaat tcgaagaaag acaactaatt gtacttttca
     4561 caaaaaaaga cagagggtcg tgacaaaatt gtgtcacaac cctttttctt tattgtgcag
     4621 tttcgatttc gcctagcgct gcttgtgcaa gtaggttcaa gtaattccat gggcggtcaa
     4681 aatgtggttg gaagaagaaa tctgaaagtg cgagatcttc tacagtcatt ttattttgaa
     4741 cagctaaaga catggtattt gctgattgtg taatgtcgta tttggacatg aactgagcac
     4801 ctacgatacg attcgttcct ttttcataga ctagctccat cattactttt tcagtagttg
     4861 gcatgaactc tggacgataa ttatcttcaa atacagtcgc ttggacttct agatcgttca
     4921 aagttgcgct ttctttggtc acacctgtcg aaccgatcgt ccagccaaat aggtaaagtc
     4981 ctgaagttcc ttgagtacca cgatacgcca ttttatgatc tgtcaagtta tttcccacaa
     5041 gcaaaccttg acgaacggca tttgttgcca aaggaatata gttttgcgtg ccacttggat
     5101 tgtagttgac tactgcagag tctccagctg caaaaatgtc tggattactt gtctgcatgt
     5161 attcattcac tttgattgcc ccgtttggca gtgtatctac ttttccttcg atcagttttg
     5221 tatttggacg gaagcccaca cataaaatga ccatatctgt atcaaatgta tctgttggtg
     5281 tagctacttg tttcactttt ccttcttcat ctgtgataaa ttcagtaaca ttttcgccta
     5341 gcgcaagagt aacaccgcgg tctgtcaatt ctttttctaa gatatctgtg aatggtttat
     5401 ctagatattt attcaagatg cgatctaatc catcaatcaa tgtcacttgt tttccagatt
     5461 cgacaaaagc ttctactaat tcaataccga tatatccacc accaacaatt actacacgtt
     5521 ttgcttcttt cgcttgtgca ataatttcat ttgcttgatt gtagtttttg cataggagta
     5581 catttttcgc atcgattcca ctaatcggtg ggataatagg ccatgaacca gttgtcatca
     5641 ctaatttatc ataagaaacc gtttctgttt ctcctgtttt cagatctttt gccgttactg
     5701 ttttattttc tgtatcgacg tctgttacat cgtgttccat tttaactgtt gctcctaatg
     5761 aagataattc ttcaggattt gagtagaaca agcctgctgg gtcctttact actccgccga
     5821 cataaagtgc gataccgcaa gataagaaag aaatattgtc atttctttca tatactgtca
     5881 cttctgcttc tggatgattt tttaaaatac tttttacagc tgcagtacct gcatgagtac
     5941 agccaattac tactactttc atttatggtc cctccgatta acattttgtc cttcgacaaa
     6001 actactataa cagaaataaa acggatacaa cagtttaatg cttgtgaatt cacaaaggtt
     6061 tgtttgtagt ttcattcctt ttcaagcata agaacccaat atctacaaga ttcttatttt
     6121 aataatattc acttttatat ctaagattgt gaataaaaga caaaaaaata aaaaagctag
     6181 cgtttttttt acacagctag ctttttttag gtataacact tgtagaaact gtttttacat
     6241 ttgcgccttt tttgctgact ctttacaatc cggacagagt ccatacaatt ccaaccgatg
     6301 ttcattaatc tcaaaatcag tcagtctgct tgcagccatt tctatatctt caagccctgg
     6361 gtaatgaaaa tcaacaatct taccgcagtt ttcacaaatt acatgataat gacgctttga
     6421 actgaaatca aatcggctag aagcatcgcc gtacttcatt tcttgaacaa atccaatcga
     6481 agtaaataag cgaaggttat tgtaaacagt cgcaacactc atatttggaa accgatgttc
     6541 aagtgcttta taaatctcat cagccgttgg atgggaatga tgttcaatca aatattctaa
     6601 aaccgcgtac cgttgaggtg tgattcgaat gttctctttt tttaactttt cgataacctc
     6661 ttcgactaac ttattttcca tgtaaacacc ccacgttgtt attctcgtct tatcatacta
     6721 tttttacatt ttaaaatcca
//
LOCUS       DAZUKZ010000121         6693 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000121 DAZUKZ010000000
VERSION     DAZUKZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.125"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..477)
                     /locus_tag="ACG8EF_002414"
     CDS             complement(19..477)
                     /locus_tag="ACG8EF_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4947695.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(490..1821)
                     /locus_tag="ACG8EF_002415"
     CDS             complement(490..1821)
                     /locus_tag="ACG8EF_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFS4947696.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1832..3274)
                     /locus_tag="ACG8EF_002416"
     CDS             complement(1832..3274)
                     /locus_tag="ACG8EF_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4947697.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3261..4025)
                     /locus_tag="ACG8EF_002417"
     CDS             complement(3261..4025)
                     /locus_tag="ACG8EF_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFS4947698.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4129..5004)
                     /locus_tag="ACG8EF_002418"
     CDS             complement(4129..5004)
                     /locus_tag="ACG8EF_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4947699.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5259..5343)
                     /locus_tag="ACG8EF_002419"
     tRNA            complement(5259..5343)
                     /locus_tag="ACG8EF_002419"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5307..5309),aa:Leu,seq:caa)
     gene            complement(5361..5432)
                     /locus_tag="ACG8EF_002420"
     tRNA            complement(5361..5432)
                     /locus_tag="ACG8EF_002420"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5398..5400),aa:Gln,seq:ttg)
     gene            complement(5442..5514)
                     /locus_tag="ACG8EF_002421"
     tRNA            complement(5442..5514)
                     /locus_tag="ACG8EF_002421"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5479..5481),aa:His,seq:gtg)
     gene            complement(5576..5660)
                     /locus_tag="ACG8EF_002422"
     tRNA            complement(5576..5660)
                     /locus_tag="ACG8EF_002422"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5624..5626),aa:Leu,seq:caa)
     gene            complement(5681..5751)
                     /locus_tag="ACG8EF_002423"
     tRNA            complement(5681..5751)
                     /locus_tag="ACG8EF_002423"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5717..5719),aa:Cys,seq:gca)
     gene            complement(5797..5868)
                     /locus_tag="ACG8EF_002424"
     tRNA            complement(5797..5868)
                     /locus_tag="ACG8EF_002424"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5834..5836),aa:Gln,seq:ttg)
     gene            complement(5880..5952)
                     /locus_tag="ACG8EF_002425"
     tRNA            complement(5880..5952)
                     /locus_tag="ACG8EF_002425"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5917..5919),aa:His,seq:gtg)
     gene            complement(5955..6028)
                     /locus_tag="ACG8EF_002426"
     tRNA            complement(5955..6028)
                     /locus_tag="ACG8EF_002426"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5994..5996),aa:Trp,seq:cca)
     gene            complement(6035..6115)
                     /locus_tag="ACG8EF_002427"
     tRNA            complement(6035..6115)
                     /locus_tag="ACG8EF_002427"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6079..6081),aa:Tyr,seq:gta)
     gene            complement(6117..6192)
                     /locus_tag="ACG8EF_002428"
     tRNA            complement(6117..6192)
                     /locus_tag="ACG8EF_002428"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6157..6159),aa:Phe,seq:gaa)
     gene            complement(6228..6300)
                     /locus_tag="ACG8EF_002429"
     tRNA            complement(6228..6300)
                     /locus_tag="ACG8EF_002429"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6265..6267),aa:Asp,seq:gtc)
     gene            complement(6310..6382)
                     /locus_tag="ACG8EF_002430"
     tRNA            complement(6310..6382)
                     /locus_tag="ACG8EF_002430"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6347..6349),aa:Val,seq:tac)
     gene            complement(6398..6469)
                     /locus_tag="ACG8EF_002431"
     tRNA            complement(6398..6469)
                     /locus_tag="ACG8EF_002431"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6434..6436),aa:Glu,seq:ttc)
     gene            complement(6484..6573)
                     /locus_tag="ACG8EF_002432"
     tRNA            complement(6484..6573)
                     /locus_tag="ACG8EF_002432"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6537..6539),aa:Ser,seq:gga)
     gene            complement(6613..6686)
                     /locus_tag="ACG8EF_002433"
     tRNA            complement(6613..6686)
                     /locus_tag="ACG8EF_002433"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6650..6652),aa:Asn,seq:gtt)
BASE COUNT         2007 a         1484 c         1099 g         2103 t
ORIGIN      
        1 aacttttttc atcgtacact aaaattcaaa acaactatct ttaatcttta cttcttggtt
       61 ttgttttaat aaatttcctt caaaaatgcc tggagaaact acaatgatca ccgtatcttt
      121 ttgtgcttgc tccaacttct tcaaatctat aatagctaac agtgtccctt cttttacctc
      181 ctggttactt tcaacccgta tctgaaacgg acttccttcg agttcaactg tatctaaacc
      241 catatggatc agtatttttc tgccagattc actttcgatc gttatggcat gtcgggtagg
      301 aaatatatta tcgattattc ctttaactgg cgcatggata tatccatcat gattcgttat
      361 agcgtatcca actcctatca tttttgaaga aaagacctcg tcttttactg ctgttaatgg
      421 aataatttga ccactagcta cagcttttag aatgtttctt gttttaaaga aatgcacttt
      481 ttatcacctc tactttaatg ctggccaata ttttttgttt actttgatca tttcatctag
      541 tatttttttt gcgactctgg catttggtac ggtttgattc aatgtaaatg cttgtaaagc
      601 tttttgataa gaaccttcaa agtaagcatc tactagtaac ttctctactg caacttgtgc
      661 ctccatcaat cctttatgaa aatcactgat tggttttctt agtgtaattg gctccacacc
      721 agttgcacca acatatgccg gtatttcaac aactgcatct gaccgtagat tcggaatcgc
      781 cccttgatta ggaacgatta gcataaagcg acgacgatca tcattcaaca aggaaatagc
      841 tatatcgacg atatattgtc catgctcccc aaaattaaag ttcaatactt cttccacaat
      901 accgcggcgt attttatcag ccatttcttt ggtatttttc tctcgtccat ccatgaccat
      961 attagcacgt gtatactctt tgtcagtatg ctcaacgacc atatcaggat ataagtaata
     1021 ctctaagtaa ttattcggga tattacttgg aaaatttttc gtgatgactg acatcatttg
     1081 aaaggttttt tgccaagttt tatctcctat attaaaatcg gcgacttgca tttcttttgt
     1141 tattagtttt tcaataatct ctggcataat ttctcgttgc aattctttat cataaatcga
     1201 tgtataccag ccaaagtggt ttaaaccata atacgtaacg atccaattct tacgatcata
     1261 accgtaattc actgcaatcg tttcttcaat tgaaatagtc atatcacagg cattaatcat
     1321 ttttacattc ggatatttac gacgaacagc ttcagaaacg atcgactctg gatttgtata
     1381 attcaaaatc catgctttgg gggcatagtc ttgtatatga tcgacaattt ccaataaacc
     1441 tttgattgag cgcatcccat aagcaaatcc tccgagtcca cacgtttctt gtcctactaa
     1501 tccatgttta agtggaatct tttcgtcctt ttctcgcatc tccaatcctc caacacgaat
     1561 ttgtgagaaa acaaaatcgc atcctgtaaa agcaagcttt ggatcatccg ttgcacgaat
     1621 gcgaatattt gaaaaacctt ttcttttcag cataaattta acaatcaagt acatatccat
     1681 atttctttct tcatcaatat catataaacg tatttcagat aatggcaacc gtttttcatt
     1741 gttcaaaatg gcttgaacga ttcctggtgt atacgtactc ccgcctccag ctatagtgat
     1801 aatctgtttt ttcatcatca tcttttcctt tctattcttc taataattta ttgaattctg
     1861 ccataacata agtcacttct aagccgatga taatttgata atttttttca gttggtttaa
     1921 tgatcccgct tatccctaat tgcttgattc gctgttcctc gaccatggtg ggatccacta
     1981 cctctacccg taggcgtgta ttacaactat agacatccag gatattggct tttcctccaa
     2041 acgcttgtag catttttgtt gccatatatt cataattttt acttgctaac cctaactctg
     2101 tttcttctaa ggttacttca ttgccaaact ctgccttagc ttcacgtcct aatgtctgaa
     2161 tgtttctctt attgatcaaa taataaaaag taacgtaata caaggcaaaa aagaaaattc
     2221 cgattggaag aatcaaaata gcattggtag cgattcgaaa attgatgaga taatcaacaa
     2281 tacatgctcc gaatgtaaaa ccaattcgta tattgaataa gtaacagaca atccctgcta
     2341 aaccggcaaa aagtgcatgg atcacgtaca acataggaga aagaaacatg aaactaaatt
     2401 caacaggctc agtgatattt gcaataaatg aagttcctgc accgcttccc atgattcctt
     2461 tgaccatctc tttattttct ttataagctg ctttatagat agctagacat atagctggta
     2521 taccaaacat cattactaca aaaaaaactg ataaaaacag tccagcctga ggatctccgt
     2581 tgataaagcg tgtcatctct cctctgaaaa cttccttgct aggagatgta tagcttccta
     2641 aatcgtaata aatatacgta ttcaacacat ggtgcagtcc aaaggggatc agcaaacgat
     2701 tcaaaaacat aaatatcccc acacctaatg ctcccatatc aacgagcagt tttgcaaaat
     2761 tatcaatccc aatttggatg agcggccaga taaagcccaa aaaaacagct gcgattgttt
     2821 gtagaatcat caccatagtc aatgggaatt tttcgcccgc aaaataagaa aaaactactg
     2881 gtaatttttg ttctttaaag cgattatacg tccatgccgc tactaaaccg gaaataattc
     2941 caccaaaaat ccccatattc acttctgggt ccataatttc taagcctttt ttcaaggtag
     3001 caatcgctaa aaaaccagta agtgcaggta ttcctttgtc cttatttttg acaaaaccga
     3061 ttgcaactcc tatagcaaat aagatatcca agtttccaaa aacggcattt ccagctgcac
     3121 caattactgg gatgttcagc atatcgtcag ctgataaacg catgatcaat cccgctaatg
     3181 gcatcgctgc aactggaagt agcatcgctc ttccaagctt ctgcacattt gatttaacat
     3241 tcatttttat tcctcctcta ttaaatatcc tcatatgtta ctaactcaat caaaggatca
     3301 tttttcactt tttgagcaaa tgctgaatga attaaaaatt cgacttggtg ggtacgagga
     3361 tacacaaaac ttgaaccatt taacaattcc tgatccaaat acgctgcgtg acacatgact
     3421 tcgatcgttt tatcttctct caatatcatg agtaaacttg ccaaatattc ttccacactc
     3481 gtattgattt gtcgtttttt ttcatcagtt tctcctacga catcgaaaca aggctcaaaa
     3541 taatcgacat gacgaacttc atcttttcgt tcaaagttgc tccgaaccgg caaatcatat
     3601 ttttttgcta atgtaacaac tacctcctga ttttcatcca aagtatgagt atgatgatga
     3661 ctgtctaaat gagttggtat gatccccgca tgataaattt tttgaatctg agcattccat
     3721 tcctgataca gttgatcttt gtcaatgtta aatggatgct gataataaga taaggatctg
     3781 aactctccgc tttcgataag agtatcgaca gttttgagga gtggtttgcc acacgtcaat
     3841 gttaaatgga ctcctacacc tagcctaggc atttcctttc ttagtttcac agcatgctcg
     3901 aaacccggca tatttgccat caatgtagtt gatgtcaaaa ttcctcgctg ataagcatcc
     3961 ataatcccat aattcacacc atggctatag ccaaaatcat ccgaattaaa aatcactttt
     4021 cccatttcat tctccttctt ttttctacta aaataggcgc aaccaaataa tcatagtcca
     4081 tttactatgt gtcatttggt tgcgcctata gatagttacg cccttatttt aatagattct
     4141 tgctagatga attgccaaat agatgatttc attttcttga atggcaagtt gaaattgctg
     4201 ttcgatgata tctttaatct ttagtgctgt acaatacgct ttgctatgtt taaatgtttc
     4261 agtaaaaaaa ccaatatttt ctactggcgt ttcttcctga taaatcaagc gtttaaccag
     4321 gaatttgata tgaaccatta agcgatgata agtcagtgat tctttatcat aaacgtttga
     4381 ataggatttt tctagatggg acaaaatcgt ggtcgtcatt tctactattt ttaagctctt
     4441 taaactatta ggctctccta gctcagcatt aatgatatgc atagtgatga atcctacttc
     4501 atttgaccct aactcggttt gatatcgatg attgattcgc tcgaccactt ctactgctat
     4561 atcatattca tttggataaa ggaatctcat ttctggcaac ataattgctg tgacatctct
     4621 atcttctctt tttcgttgaa tagcaaaata aatatgatct gtcaatgtaa tatagatatt
     4681 tgaatctaat ggagaggaga tcctagctgt tatcagttca atcacttctt ccgcaaaatc
     4741 cataaattcg attggaatct gcgagactat ctcactgaat cgtttgatct tttctttaga
     4801 atctaataca aataatttgt cagcatcctc aacatcaaat ggcttactca ctttagcaga
     4861 aaagccaatc cctttcccca tcgcaatttt ttctattcca tcgtcatcga tcaatacagc
     4921 attatgattc agctttttca tcacatacac cgttaattca cctcctctat atgataaaac
     4981 aaaacaactt gcagactcct tcatacatat ttttgtctcc aaacaatttt taaagacaaa
     5041 aaacgaccta agaaagaacc ctttcttagg tcgtgcctaa ttgaatagta gcaccctaca
     5101 taatttacaa aaatagttta acacataata aaagcgttat caatatcttt tttactcgtt
     5161 ttatgataat acttgtatcg aattgatttg gaggtaatta cataaaaaca aaaaaaatat
     5221 agcaactctc tataagaaag ttgctatatt gataattata ccggcggccg gggtcgaacc
     5281 ggcacgtcct caacggacac tggattttga gtccagcgcg tctgccaatt ccgccacgcc
     5341 ggcaaattta aaaataataa ctggggtagc tggattcgaa ccaacgcatg agggagtcaa
     5401 agtcccttgc cttaccgctt ggctataccc caagaataaa gggcgaatga tgggaatcga
     5461 acccacgaat gccggagcca caatccggtg cgttaaccac ttcgccacat tcgccatcat
     5521 aaaagatgtt atttaatttc tcaataaaaa actaagatga agacatctta gtcaataccg
     5581 gcggccgggg tcgaaccggc acgccctcaa cgggcactgg attttgagtc cagcgcgtct
     5641 gccaattccg ccacgccggc aagttgattt attagtacta aggcggtaac cggatttgaa
     5701 ccggtgatga aggttttgca gacctctgcc ttaccacttg gctataccgc cattatacta
     5761 ttatcaatct ataaaataaa gaataaatta tagaaactgg ggtagctgga ttcgaaccaa
     5821 cgcatgaggg agtcaaagtc ccttgcctta ccgcttggct ataccccaag aataaaaagg
     5881 gcgaatgatg ggaatcgaac ccacgaatgc cggagccaca atccggtgcg ttaaccactt
     5941 cgccacattc gccatggcag gggcagcagg aattgaaccc acactaacgg ttttggagac
     6001 cgctgttcta cctttaaact atgcccctat aaaatggagg agagtggatt cgaaccactg
     6061 aaccctaagg aacggattta cagtccgtcg cgtttagcca cttcgctact cctccatggt
     6121 ggcgcgggac agaatcgaac tgccgacaca cggagcttca atccgttgct ctaccaactg
     6181 agctaccgcg ccatatttta aaattattaa ttgaataaca agaacgacgg ttccgacggg
     6241 aatcgaaccc gcgatctcct gcgtgacagg caggcatgtt aacccctaca ccacggaacc
     6301 agttcattat ggaggttaac gggatcgaac cgctgaccct ctgcttgtaa ggcagatgct
     6361 ctcccagctg agctaaacct ccaacattca attaaaatga cccgtacggg attcgaaccc
     6421 gtgttaccgc cgtgaaaggg cggtgtctta accgcttgac caacgggcca ggtaaatttg
     6481 ctacggagag taagggattc gaacccttga gacagtttgg caccatctac atgatttcca
     6541 atcatgctcc ttcggcctct cggacaactc tccagagtga aagaagctca atactctttc
     6601 tcaaaatgag aactccggca gtaggactcg aacctacgac atcatgatta acagtcatgc
     6661 gctactacca actgagctat gccggaataa cat
//
LOCUS       DAZUKZ010000122         6644 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000122 DAZUKZ010000000
VERSION     DAZUKZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.126"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG8EF_002434"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG8EF_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8EF_002435"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8EF_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFS4947700.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG8EF_002436"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG8EF_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG8EF_002437"
     CDS             complement(2143..2373)
                     /locus_tag="ACG8EF_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFS4947701.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG8EF_002438"
     CDS             complement(2603..3421)
                     /locus_tag="ACG8EF_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4947702.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG8EF_002439"
     CDS             3582..4271
                     /locus_tag="ACG8EF_002439"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS4947703.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG8EF_002440"
     CDS             4285..5787
                     /locus_tag="ACG8EF_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4947704.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG8EF_002441"
     CDS             5800..6276
                     /locus_tag="ACG8EF_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4947705.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG8EF_002442"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG8EF_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAZUKZ010000123         6633 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000123 DAZUKZ010000000
VERSION     DAZUKZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.127"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(387..632)
                     /locus_tag="ACG8EF_002443"
     CDS             complement(387..632)
                     /locus_tag="ACG8EF_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4947706.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(626..1027)
                     /locus_tag="ACG8EF_002444"
     CDS             complement(626..1027)
                     /locus_tag="ACG8EF_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFS4947707.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(1199..3007)
                     /gene="uidA"
                     /locus_tag="ACG8EF_002445"
     CDS             complement(1199..3007)
                     /gene="uidA"
                     /locus_tag="ACG8EF_002445"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFS4947708.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            complement(3035..4801)
                     /locus_tag="ACG8EF_002446"
     CDS             complement(3035..4801)
                     /locus_tag="ACG8EF_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFS4947709.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            complement(4814..5608)
                     /locus_tag="ACG8EF_002447"
     CDS             complement(4814..5608)
                     /locus_tag="ACG8EF_002447"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4947710.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            complement(5619..>6633)
                     /locus_tag="ACG8EF_002448"
     CDS             complement(5619..>6633)
                     /locus_tag="ACG8EF_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4947711.1"
                     /translation="GSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATLAGMFI
                     GVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKSDESQL
                     KVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIVLMNFI
                     NPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLVLTSVG
                     YMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVGGWTLS
                     IIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENYNLLNQ
                     KHN"
BASE COUNT         2111 a         1286 c          838 g         2398 t
ORIGIN      
        1 tgcctcactg aatttggtct taaaaataat agaaaaaatg ttgaaatcaa catatggaaa
       61 taattcttaa tcagctgctt ttgtcaaaag cacataggaa tttcacctct ctccaagttt
      121 acggcgagcc ttacggaagt atcattatcc caacttcaaa tcatcgcttt ttattgacta
      181 taaaaattat tttagaatgt ttatcgctcc ttctttacag taagaaatca tggcgcaact
      241 aaaatatgct ccttgaagtg ttaacgtact gatagaatgt atttaaattt caatgatcaa
      301 taggttttct gtttaaaaga atctactaat cctttaacta ttagaagatt cttttaaaca
      361 gaataagtgg aaaggggact ttttagttag ttaattttaa attttaagat cgcttctatc
      421 aatttaattt ctagctgtct tagcatatac tcatctacat aggtaataaa ttgccccgcc
      481 tcattaaatc catttgtcaa acataactta gctatataac tatgataatg tttgagtatc
      541 atcttgattg aaattggatc ttcttctaga gcagattgga taatggatag aggaactaat
      601 ggatactgcc gttttttggg gttgtctacc ataagtcata ccccattttt aattgttcaa
      661 ctgccttctt tcgttgatac cagataccac ttgtactcat attgaggatt ttaccgattt
      721 cagtatcatt aaaacctgca aaataataaa gtaggataac ctttcttctt atttccgata
      781 aatgatcaat ggctgccgat aacttttgat ctgaaattaa tatttccatt ccaaataaga
      841 aatagacatc ttgttcatca aaatgagttt gggagtactt accttcattt gaattcgtca
      901 atggttcctc attatctgtt atgttaatgt ttcgttttcg aatagactta gtttcgtttc
      961 gtaatacttt tttacagtag gcatcaaatt gatgttgtat agatttctca ttgtaatttt
     1021 gttgcatgat aaatatgctc cttttttccc ctttccacta atacaacaat cagaaagcgc
     1081 ctttttttcg aaaaaactag aaaaaaatat ccttctttaa acagaaaatc tttttataaa
     1141 aaaaaggagt tggcaattaa gcagccactc cttttttata ttccttataa aaggtaaatt
     1201 attttttata actgaaattt ggtattgatg accatcgttt ctttagatag tgagcaatca
     1261 ttttaggctt tctatctcta gtaaagattc ctttcttatt tccttgcact cttaaaatcc
     1321 cttgacttgt ttcaaaatct gcaaaattcc acacttgttc accaacaaaa ttgtcacatt
     1381 tatctattac ttcgtgattt gcttcgtaat aacccacttg atactcttct gtaaacattg
     1441 ttggtactgt atcatgaagt ccagctactg tatctgctcc atattctgta aacattattg
     1501 gcgtatcggg acatctttcc atccatccat ctagctcttt tttcaacaga tattttgcat
     1561 gttttaggtc tccacctgaa acataccaac cataatagcg atttaaacat aaaacatcga
     1621 caatatctcc tactttacaa atatctggtg ttgactttaa gtaagtaacg attgttacag
     1681 gtctcttctg agggtcaagt tctttagtca attcaactaa aggttcaaaa tattctttag
     1741 cgccttcaga atccgaatct ggttcatttg caatagacca cattacgaca ctcggatggt
     1801 tcttgtcgcg ttctatcaat tccttaatcg ccattttgtg agcattttct gtccgtattt
     1861 ctttccaagt atcatgtttt tctacgttgt catctaataa agtagccatg aaattcaaat
     1921 gtaatcctac tgcaggtact tcatcaataa ctacaatacc ttctcgatct gctaagcgca
     1981 taatttcttc tgaataagga taatgtgaag tcctaaatga attagcaccg atccatttca
     2041 ttaaattaaa atctttgata tttactgctt cattcaaacc acgaccatta atatacgtgt
     2101 cctcatgctt cccaaatccc ttaaagtaaa aaggtttatt attaatcaaa aacttcccat
     2161 tcgaaacctg aatactacga atgccaaatt cttctgtgta cgtatcaatc actttatttt
     2221 cgttaattaa actaactttc atactatata agtaagcatt tagaggctcc caaagtgttg
     2281 ggttcttaag ttgaatcact ccttctttcc ccttagcttc tgctataagt tgagcttctt
     2341 cgtcatagat tgctattaga atttctccgg tccattctgt atcgatatta aattctacat
     2401 ttgcatctga atcaattatt tttgtattaa tagtgacatc ctttatatag tctttcggag
     2461 ttgtatacaa tttaacgggt ctattaatgc ctgcataatt gaaaaagtca aaattaacca
     2521 aattttcttt ttttatgtct ccatcttcct gtatcgtttc tttcaaaatc cctacaggta
     2581 aagtgctatc gtcaataaca ttgtttacag ccactgtaat tctgttactt ccatctttta
     2641 taaactgatt aatttctgat tcaaatggcg taaaccctcc tttgtgttga ttaatcaatt
     2701 ttccattcaa aaatactttt gcttcatgtg ttgcagatcc aaaacgaaga actaatcttt
     2761 cttctaataa ttttttagga agaatcactt ctttttcata ccacacccat ccaacatgat
     2821 ttctaatgtt ctctgtttca aataaatcat tataagaaga aggaactgaa attggataac
     2881 tctctttttc aaccattggt tctaaagcaa gttcttcttg taagtcatca tttgtatcta
     2941 acttaaatct ccaaagaccg cttaaatcaa ttacttgcct cgtctctgtc ataatgggat
     3001 ataacatgta aatcactcct atataattta aatattaaat aaatgtatcc tttaacccac
     3061 aataagcatc tattggactt attcccttaa attcagaaag gcccattagt ttgtccacta
     3121 atatttctaa agtttcttgt tttccatcat aagtattgat ataagttttt acttgaggca
     3181 catcagctaa atgaaacgga cactgtacag atacaaaaat tgttggaatt tcatgaatat
     3241 aaaatggaat atccggagta cctttacttg ctggccaaac aattctttga actgtacctg
     3301 ggttaacatt agctatatta ataatcaagt catatttttc agttaaatct ttgattggtc
     3361 tcttagcagc atatattcca gctatatttt taattttttc ttcttgcgat aatcctttca
     3421 attcttcttc aggtgaaatg tatttttcaa cttcaaatcc ttttgctgac aaaatctgtt
     3481 tcaataaatc aatggcctga ggtctatttc cagcaactac ttcaccaaac ccacctttta
     3541 ccccatgtac atctaccagt aaaatccttt tatatttttc tggtacaata ggccaaatat
     3601 cttgtttatc tttaactagt gtaattgctt gatcagctac ctctttaaaa attgatttat
     3661 tttcaattaa accaattttt tccatagctt cttgtttcgg taacaaaatt ttattctttt
     3721 cttttttatg caaacctagt ttagccttta agcctaaaat tcttcttaat gcatcacgta
     3781 aacgctcttc tgaaataatt cctttgtaat aaccttccat catataatta aagtcttcat
     3841 ctggatcatt aaaaaatagg aatagatcac atccagccgc aatagaagtg ggcaataatt
     3901 ctgaccgttt cattgcccca gtcattccta acatatgact agcatcagta actactaatc
     3961 cattaaaccc gagttttcct ctaagtaagt cagtgattaa ttcttttgat aaagttgcag
     4021 gaagtaaatc ttcatctttc agatcaagat taaaatgttt tgaataagct ggcatatgga
     4081 tatgacctgc cataattgaa ggtaatccag cttctattaa gcctgagtat acttttccaa
     4141 aagaattatc ccattcttct gtacttaaac tattaatact aaaagataga tgttgatctc
     4201 gttcgtcaat accgtcacca gggaaatgtt tagctgcagg caaaattcca ctctcttgaa
     4261 tacctttcat ataagctaaa ctaagttcta aaactttatc agggctttgt ccaaaagatc
     4321 gactagaaat gataggattt ctccaatttc tattaatatc tacaatagga gcaaaactcc
     4381 agttacatcc aatagccgat gcttcaattc cagaaactct ccctaattca taagcatatt
     4441 tcgcatcatt tgttgcacca atttttactt ctacaccaac ttcggtacca tctgtacaag
     4501 ctccgttacc acctgcttct gtattagcag caattaataa aggaattttg ctttctgttt
     4561 gtaggatata gttttgatca tatacctcat cagcagttcc tggattatat cgtacagctc
     4621 caatatggta ctgattgacc atactactta agtaattttt atcacgacta gatcccatat
     4681 taacaaataa ttgacctatt ttttcttcta aagtcatagc atcaatcgtc tcttcaatcc
     4741 aagaaatagc atcttcatct aaatgatatg gtttttttcg taagtctact tttatagtca
     4801 ttttctttct cctctactta ttagtaagat ttttccaaaa agtatctata tatttttgtt
     4861 caatatctat atactctccc gataaacatt taaataaaat atcagttctt aagttttccc
     4921 atgattcttt ttctttatta accaaaatag gataaaagaa aactttattc ttatttgcag
     4981 cttctagatc accaggagaa tcaccaatca ttaatatctt atccaaatca caaccatttt
     5041 caattacact tcgaataatg tcttcttttt ttcctttatc ttgacaacac agttcatcaa
     5101 ctaaggataa taattcatgt ctttcccatt cttcttgaat agcttcctta ttagctgagc
     5161 ttacaataat tatcttccca tattttttca gagcttttat agtatctcta gcacttaaaa
     5221 agggtttatc taatccttta gcttgactaa tctgtcgatt gacttcttcc gaccatttta
     5281 aagcttttaa taaatcctct gactgagagt attctatttc cctcttcaaa gatggattag
     5341 acaattcttt tgttacagat acccagtgct ttaatcgatt aatcgatggt acatcatttt
     5401 tttctaatgt tttaactaaa cctaaaaatc tgtttattcc acgagtctct gaatataaat
     5461 taatattatt ccaatcctgt aggaattttt ccctatgatg aacatcaaaa atctctgcag
     5521 caattggccc gaaaaagaga ttgtgtttat aagtcattgt atccatgaca caaccatctg
     5581 aatcaataca aaatatccaa ctgttcatgt tttttctcct agttatgttt ttgatttagc
     5641 aaattataat tttctaatac tttttcttta gataatggat atacgaaaaa taaaatcaat
     5701 cctacaacaa agaaacaaac tgctggaatc aacgttgaag agttatacag cttttgtaaa
     5761 acttctccac tttgtacttt tgctaacgaa tcatacccta ttatagaaag agtccaacct
     5821 ccgacaccgc cagctaaagc ttgtccaatt tttcgggcaa aagaatatac tgcataaata
     5881 gttccatctt ctctttggcc agatttcact tcttgatcat cgattacatc agtaatattc
     5941 gcccaaatga ctgtattaaa aatacctaac cccatatatc caacagaagt aagaactaag
     6001 aatacataca tattactagg ctttatcaaa aataaaagta aatatacaat agcagagaag
     6061 aacatcccta ctgacgccaa ttctttttta ccgtattttt tactgaaaat taaacttaca
     6121 ggtgaaacaa gtaccaacat caaaatagga ttgataaagt tcattaaaac tattccctgt
     6181 gcattattat aataatctgg aaatacataa ttgttcatac tcattgttaa aagctgagca
     6241 agcaacaaaa aaatagctgc agctattata gaaagcaacg ctctattttt agctaatcct
     6301 tttaaagatt tcaacaatga aacttttaac tgcgattcat cacttttaac ccgttctaca
     6361 gttaatttat aacatattac gtaacaaata actgcacaca ctgaaaatac tcctgccaca
     6421 attgtaaaag ttgatccacc acgtacaatt tgagctccag aactatctct tgtataaata
     6481 aaaattggtg ttaacacgcc aatgaacata cctgctaagg tagctcctat actacgaaat
     6541 gttgataacg ctgtcctttc ttctgcaaca gaagtgatag ctgatgccat tgaaccgtaa
     6601 ggaatattaa tagatgtata aaaaatactt ccc
//
LOCUS       DAZUKZ010000124         6620 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000124 DAZUKZ010000000
VERSION     DAZUKZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.128"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..2811
                     /locus_tag="ACG8EF_002449"
     CDS             136..2811
                     /locus_tag="ACG8EF_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFS4947712.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2993..3232
                     /locus_tag="ACG8EF_002450"
     CDS             2993..3232
                     /locus_tag="ACG8EF_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947713.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3248..4621
                     /locus_tag="ACG8EF_002451"
     CDS             3248..4621
                     /locus_tag="ACG8EF_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4947714.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4625..6259
                     /locus_tag="ACG8EF_002452"
     CDS             4625..6259
                     /locus_tag="ACG8EF_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4947715.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6422..>6620
                     /locus_tag="ACG8EF_002453"
     CDS             6422..>6620
                     /locus_tag="ACG8EF_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947716.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2321 a         1069 c         1329 g         1901 t
ORIGIN      
        1 aaatcacaac ttttttcttt tgtcaagatt ttgagtggga tatatttttg tagaactgaa
       61 gatcatgaaa ttatgagagg ttggaggtaa tgtagtcata caaataaaaa ttttttagaa
      121 agtgagtggt aattaatgaa aatgaatcac acttcaattc agaagcattt acaactgtta
      181 aatacactgg ggatttctta tgttaatttg agaacttttt ctacattaaa caagaaaacc
      241 gtttctcata ttttttctac tgcagattct caagcaatta ttaactattt agaaaaacag
      301 aaattgtctg aaggtacagt aaacataact tataatccta ttaaacaaga ggtgattaca
      361 caaaaaaagc attctgtgag agacaatgat attgaaaaaa ttcggtttgt atttattgat
      421 attgacccta aaaggaaaga gggaagtgca acagatagcg aaaagaaaaa ggcagaaaat
      481 gttatggaac aagtagaacg ttatttgaaa gaaaagagaa tagaatcatt tgtaaaagtt
      541 gatagtggga atggctacca tatttttctt cccataaatg aacaaccaaa taatcacgag
      601 acgattctta ctattcagaa ttttcttcaa ttacttcacc gaagatttgg agtagaagat
      661 gaagttgata ttgatacttc tgtttataat ccttcaagat tatgcaaatt agttggtaca
      721 ccagcggtca aaggaaagtc cacaaaagag cgtccgcatc gtttatctag tttcctttct
      781 attcccgata acatagatag aagtaattgt tttaaaattc ttgaacagat agttgaagaa
      841 aattccatga tgtcaacctc tagagtaaaa agccatccat cttttagaga agtgttacct
      901 tttattcaag cagactataa acaatggtta gctcactatc ctgaattaac ttataccata
      961 aaagagggcg atcataaagg agttacctta cttatatttg acgaatgtcc attaagaaaa
     1021 cacacgaata attctaatgg ttcttgtttg tctgttacgg ctgataataa gattcgattt
     1081 cattgtctgc atgcttcgga taaaaataaa gatattcaag actttgtaaa aaaatatccg
     1141 ttgccaaaac ctgtaaatcc ttctgtagaa tcattagaat ccaatcaaga atattatttt
     1201 gaccagttta aactgaatca agaaggagta tttttggtga aagaaggtca agaagaaaaa
     1261 gttgcttgtc cgatgtttat taagcaaata cgcaaaaata tcgaacaaaa agtcattaca
     1321 cttgttcttg cctattatgt cgatcattgt tgggatgaaa tagaaataga aggtggatgt
     1381 ttacaaacta ataattttaa acaattagca aaacatggct taatcattaa agctagaaga
     1441 gaagctgaag tcgttgactt tttacagatt caacgggaga atgctccaat cttttttgaa
     1501 catgaatctc ttggctggaa aaaagaaaat gaagcaatga tttttcagtt acaagattct
     1561 tttggacgtg aaattccttc tgtattaact aaaaactcat gctaccaatt aacaactaat
     1621 ggtacagaag cggaatttga cgcattggtt cataagtatg ttttaggaac gaacttagaa
     1681 cttgcttggt cgattggtat tgcttctatt gtgttaggct atttaaacgt tacgaaggct
     1741 aatacttttt tgagtttact aattaaccta cagggcaaga gcacaacggg taaaaccact
     1801 acgttacact ttattgcctc tttatttgga aatccaacta cggttcttag aaatatgaat
     1861 gctaccaata aagccattat aaagctagca tcaaacaatc agggaggtgt tccactaata
     1921 ctagatgagt taggagcagc aactcaaaat aactttagct cgttgatcta tcaattagct
     1981 tcaggagaag aaaggctaag attagattct aattgtcagc ttatgccaca agagactttt
     2041 tctgttttga cccttacctc ttctgaagag cgtttggaaa attatttcaa tgagaaatta
     2101 actggtttga aagttcgata tatcgaattt tctgatatca agtttacaaa ggatgcgaat
     2161 cacgcagaga aagttaaaag tctttcttct aatacttttg gttggggtat aaagttattt
     2221 cttttgaaac tctttgaaca agggattaca aaaattgaag caacttttgt cgaagctcaa
     2281 aaaaaactat gggctgtttt acctgaacat tctttgaagc aacgcattgg taataatcta
     2341 gctttgattt atagtggagc cctcctaatt caagatatac tgggctatcc tttacgatta
     2401 gagcatatac agacggtgct tattaattcg tacgaacagt taattgtaga tactagttta
     2461 ggaagtgagc aaacggtatt tgaccgagtg aaaacattgt tggtgatgaa tagccataaa
     2521 tttttaacaa acagtgacaa caaacatacg cccagtacga tttggggaaa agtatcaatt
     2581 caaaaaaatg gggatattaa ggtaaatatt tttcctatcc cgtttgagga cttgttaagg
     2641 cgagaatttc aagtaaagga tctaacctat ctttttaggc aattgcttga ttcgggtaat
     2701 atgtgtgcag aaaaagggag aagaacgaaa cgtattcaca tgaatcgtca agttcttcca
     2761 acatacgaga ttctattacc ttcttcactc aaatcgtatt ttcgattgta atttagaata
     2821 aaccaaagta tgaaagtgag gcaccatgtt atggaaatat ttcttattga tttagcaaaa
     2881 caactgatta cttatctgta tgatagcgag caaacccaaa cggaagtaaa tgaagcttga
     2941 tctatgcttc atttacttca ttcaatcaaa catgaaagaa aggaaaattc taatgactaa
     3001 aaataaaatg acactaaaag cagaggttct tctgtatatt caagaacatt tttcaaatca
     3061 agcctttttt acaaaaccaa tttacttgga tttcgaaata agaggggtat ctgctggatc
     3121 tatcgggggt actcttcaag cgttgaagaa cgaaggatat ttagaaaatc actttgtgca
     3181 acgttcgttc aatagaagag tcgtgaaaaa atggtattta gttcattcat agagagggga
     3241 atcaagcatg cgtgtggctt gttatgatcg aacgtctgtt tatgaagaag cgaaaattag
     3301 taatgacgct caatatgatg cagtgaaaga gtatgtttta gagaaatatg aacaaattcc
     3361 ccaacgattt tcagacaaag gagaatcagg ggaaaaagcg gagcgtccag cttttcgaca
     3421 gcttagagct tgggtttgtg cgtcagaaga agagggggtg atctatgttt ggcgttatga
     3481 tcgattggca cgtcaagcca aaatagcctt agaatttatt gagttgtgcc aacaacatca
     3541 ggttcaagtg tattcgattt ctgaacctct tcctgcttct ttctcacacg cgtcaacgaa
     3601 taaactattt gtgcaattat tgttcacttt tgcagaacaa caacgaaacg tcattattga
     3661 gaatgttcgt gccggacttt cctataaaaa gcaacaacgg caatatttgt cttctgcggt
     3721 accatatggg tatcgtttag tgaatgggaa aatccaacaa gaagtacacg aagcaaggat
     3781 tgttcgctat atttttcaac tttatttgac caaaaaatat ggctacaaaa agctttgtca
     3841 acgattgacg caacaaaagc ttttcttccg cgaacgtcct tttcagccct atcatattta
     3901 tagtatccta aaaaatccat tgtactatgg cgaaatcaaa ggaggatctt tggggaagta
     3961 tctaggaacc tttgaaccga ttctctcgaa agcaaccttt ttacaagccc aagagattcg
     4021 ccaatcaagg tgtacagcta aaaaagatac gtatctgtac ttattacgtc aaaaaattcg
     4081 gtgtcccttc tgtggtcgtc atctgagttc gaagtatcaa tggaatacaa agaagacgaa
     4141 gacgcttcat tattatcatt gtaccgaccg aagctgccaa ggaatttttc ttcgggcaga
     4201 aagggttgag tcagaagtat tggaaaagat acaggctttt tttcaatacg aaggcgtcta
     4261 tcaagccttg attgcccaat taacgcaaca attagccgaa aagcgaaaag atgaaaaacg
     4321 caaagtaatc caacaaaaga aacaagaagc acaatggatt aaggcatttg aagagggcaa
     4381 ttgttcactg gaagaactca agcaacaact gcaagctctt caaaagccga tgtctacttc
     4441 ttcgtttctt tcactccaac agtgtgaagc aaaagtgaaa gaatggcttc aactaaaaga
     4501 cctctctagt cgacaattat tatgtgacta tgtttgtcgg attgaggtaa caaaggataa
     4561 aacagtttgt gggatttact tccgacaact gacacaaaat attttagaaa ggaacgaatg
     4621 atctatggaa atgatacaaa ccaaacgagt cgcattgtat attcgcgtga gtacacaaga
     4681 acaagcttta gaaggacata gtattcaagc acaagaacaa aatggacgac agtttgccga
     4741 acggatgggc tatgaagtgg tagaagttta tgccgatgaa ggaatcagtg ggaaatccac
     4801 aaaaaatcgg ttcgcctatc aacgaatgat gaacgatgct cggaaccatc gttttgatct
     4861 actagtgatt tggaaattga cccgtctcgg acgcaatatg ttagatgtct tacaaacagt
     4921 cgaggaatta ttgcgatatg acgtaggatt acattcgatc tcggaacaat ttgacgtgac
     4981 cacttcgaca ggtaaattga tgttacaact ccttgggagt ttcggtgaat ttgaacgcaa
     5041 ccaaattgcg gagaatgttc aaatgtcaat gcaaagctta gtgaaagata aaaagcgtta
     5101 tgcaggaggc cgacgtttag gctatgtcag tggtattgac acgaatggtc aaaaacaatt
     5161 ggtcattgaa ccagaagagg cgaaaattgt tcgtctgatc tatgcaaaat atttaggcgg
     5221 agcagggtat cgagcgattg ccaatttttt gaatcgtcaa ggatatcaaa cagtcaaaaa
     5281 gaatgctttt tctaccacgg cagtcaaaga tatcttacgc aataaaattt atgcagggta
     5341 tttagaatat gcgcgctaca tcaattggga aaccaaacga cggaagggga agaatccgag
     5401 accgatttta gtgaaaggaa cccatgaacc gattatttcg gaagaagatt atcaaaaagt
     5461 acaagaacgc cttgctttag aaggaaaaca acccaaatgg aaccatacag gtgaaaatgt
     5521 attgacaggc ttgttacgtt gtccagaatg tggcgcaccg atggcagcga gtaatgtcac
     5581 aaatacccta aaggatggca caaagaaacg gattcgatat tattcttgtt cagtctttcg
     5641 gaataaagga gcaagtgtgt gtcatgccaa ttcgattcgt gccgaacaag cggaaacatt
     5701 tgtcgccgac cgattaaagg agatcgttca gctaccagaa gttcttcccc gattggtggc
     5761 agcgttaaat gaagaaatag tgcgacaaag ccaaccattg gaacaagaac tcgtggtgtt
     5821 actggaacga aaggaagaat tgaagactaa aattgaaaaa tgggaagctg ctttggaaga
     5881 tagtcctgaa ttgtttccga tgttaaaaga ccgcttagac gaattgactg aaaaacgacg
     5941 acagttgcac attcgagaaa atgaaatctt agggatcttt caacaaggag agccgattca
     6001 agtcaaagat gtccaacgga tattgactaa tttggatcgt tttcttgccc agagtgagaa
     6061 gaagcaaatc aaagccttgt atcgtacctt tatagagaaa ataacctttg atcctaacca
     6121 taaggagatg ttggaaatta cgatgaaatt cacgcatgcc gtggtacaac aattgaatga
     6181 gcaatatcaa atagccgtat ccaaaacaaa ggatacggct atttttgtgt taaaaacacc
     6241 ctttacgtta acgatctagt ctaggttata tcaaacaacg ttagaggggc gctaacgctt
     6301 ctctagcgat ttgaaatcgt tagagaagta gttagggagg tccttatcat aaatagggat
     6361 atttattaat gaatatttga aatataattg tatataagaa ttaaaaagag tggggataga
     6421 tatgaaaaag atgggtaagt gtattttatg tgaaaaatac actgaactta ctaaagaaca
     6481 tgtaccacct aaaaaatcag ggaatactgg aggtaaaaag accagaactg gtaatttaga
     6541 tgatttcctt aagagcgatt ttacaaaagg tgattttccg aaggggatta aaaggaaacc
     6601 tcaaggaaat gtttattata
//
LOCUS       DAZUKZ010000125         6600 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000125 DAZUKZ010000000
VERSION     DAZUKZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.129"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..486
                     /locus_tag="ACG8EF_002454"
     CDS             <1..486
                     /locus_tag="ACG8EF_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS4947717.1"
                     /translation="LIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLKDESNTTVSD
                     ANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKTGADIDHFDK
                     ALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQETIKKLRIG
                     G"
     gene            493..1344
                     /locus_tag="ACG8EF_002455"
     CDS             493..1344
                     /locus_tag="ACG8EF_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFS4947718.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSEPKNASGAYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            1356..1625
                     /locus_tag="ACG8EF_002456"
     CDS             1356..1625
                     /locus_tag="ACG8EF_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947719.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGINNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLSELEQV"
     gene            1630..1794
                     /locus_tag="ACG8EF_002457"
     CDS             1630..1794
                     /locus_tag="ACG8EF_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947720.1"
                     /translation="MDLKTFTAQIELMHQEALRQSASYEDKWLNTFHGGRESALDQVL
                     KLLKGERRDG"
     gene            1787..2089
                     /locus_tag="ACG8EF_002458"
     CDS             1787..2089
                     /locus_tag="ACG8EF_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947721.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVTEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEKK"
     gene            2086..2397
                     /locus_tag="ACG8EF_002459"
     CDS             2086..2397
                     /locus_tag="ACG8EF_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFS4947722.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            2394..2612
                     /locus_tag="ACG8EF_002460"
     CDS             2394..2612
                     /locus_tag="ACG8EF_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HFS4947723.1"
                     /translation="MIGLTIFWCAIGGLLAFLGYYLICESRKGRKYTVTGIVLLIIGI
                     LVAVGAELNLYVNGTQEDLVRFMFWMKN"
     gene            2623..3438
                     /locus_tag="ACG8EF_002461"
     CDS             2623..3438
                     /locus_tag="ACG8EF_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFS4947724.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            3440..3565
                     /locus_tag="ACG8EF_002462"
     CDS             3440..3565
                     /locus_tag="ACG8EF_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947725.1"
                     /translation="MGFFMAKILLFLGLVGAAYLVYALLSQTDDKEDDNNDEMKF"
     gene            3577..3933
                     /locus_tag="ACG8EF_002463"
     CDS             3577..3933
                     /locus_tag="ACG8EF_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFS4947726.1"
                     /translation="MDLITQYSDIILKKIMAKIQKDKKAKERAELVKLEMAETGVGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKAHSIQHGFI"
     gene            3893..4138
                     /locus_tag="ACG8EF_002464"
     CDS             3893..4138
                     /locus_tag="ACG8EF_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947727.1"
                     /translation="MIKKLIQFSMDLYDIESGATVSVESDHLIISFADERQIIIWVVD
                     DMLYPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSK"
     gene            4135..4533
                     /locus_tag="ACG8EF_002465"
     CDS             4135..4533
                     /locus_tag="ACG8EF_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFS4947728.1"
                     /translation="MIPKFRAWDKRENTMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLG
                     HRNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            4530..4868
                     /locus_tag="ACG8EF_002466"
     CDS             4530..4868
                     /locus_tag="ACG8EF_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947729.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            4865..5095
                     /locus_tag="ACG8EF_002467"
     CDS             4865..5095
                     /locus_tag="ACG8EF_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947730.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            5102..5305
                     /locus_tag="ACG8EF_002468"
     CDS             5102..5305
                     /locus_tag="ACG8EF_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFS4947731.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASETIRKFGISILNDDRKWEAER"
     gene            5302..5607
                     /locus_tag="ACG8EF_002469"
     CDS             5302..5607
                     /locus_tag="ACG8EF_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947732.1"
                     /translation="MKLKDGFYTDAYGIGGLMMDLPTKNPIKQEESEIKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVINNGIKN"
     gene            5591..5884
                     /locus_tag="ACG8EF_002470"
     CDS             5591..5884
                     /locus_tag="ACG8EF_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947733.1"
                     /translation="MELKIDKVTFEEPAFQEMDERIAELVEKKQDELIALLEKKYQFP
                     KYMNKMQAAKYLGVSYNTMMDKYVPAGLKMGIVNGVIRISKEECDRFMKEHQK"
     gene            6144..6560
                     /locus_tag="ACG8EF_002471"
     CDS             6144..6560
                     /locus_tag="ACG8EF_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS4947734.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2470 a          969 c         1497 g         1664 t
ORIGIN      
        1 ctcataggcg atattttcat cgatacaggc atgccaacgg acttctggaa ggaattcttt
       61 tacttccgct ttgaaggtgt cacagggcgc aaaataagcc tcaaagatga atcgaatacg
      121 actgtgagtg atgctaacgt cttagcaaat atcatcttag atttcatctt tgaacatcat
      181 attcctttca aagaaggcta tgagatttta ccagcgaatc aagaatatta cttctacaaa
      241 tgcatcacaa aaagagtctg ttgcatctgt ggcaaaacag gagctgatat cgatcacttt
      301 gacaaagcgc taggaagacg aaagcgcaaa gaagttgatc attcagagta cacatttgca
      361 gcactctgca gaatccatca cacggagaaa cacaagatag gtgtgatcaa tttcaaaaat
      421 aaatatcaaa tcaaagggat caagttaaac caggaaacaa ttaagaaact tagaatagga
      481 ggataaaaaa tattggctga caacaaacga tactactatt taaaactaaa agagaacttt
      541 ttcgatagcg acgagatggt tcttttagaa agtatgccag atggatacat ttattctaat
      601 attcttctca aactttattt aagaagtctg aaacatgaag gcaaactaat gtttaatgac
      661 aggattccat tcaattctac aatgcttgcg actattacaa gacactctgt gggagttgta
      721 gaaaaagcag tacaaatatt tcgtgattta caacttattg aggtattaga taacggagca
      781 atttatatgt ctgatataca aagttttatt gggaaatctt caactgaagc tgataggaag
      841 agaaaataca gaaaagaaat tgaggaagca aaacagaatt taataacaag tggacaaatg
      901 tcggacaaat gtccggacaa aactacacca gagttagaga tagagttaga gaaagatata
      961 gagttagaga aagatataga gaatgtaacg ccttcgaaaa aatcgaaggc taagcccgtc
     1021 cgtcataaat acggagagta taaaaatgtt cttttgtcag atgagcagat ggagaaactc
     1081 aaaatagaat tccctaatga ctatcaagaa cgaatagaac ggctatctga gtattgtgaa
     1141 tcatctggta agacttataa aaactatttg gcaactattc gaagttgggc aaggaaagaa
     1201 aaaagtgagc ctaagaatgc aagtggtgca tacaagcgca caggaagacg agagaagctt
     1261 cctgaatggg caatcgacca agaagcctat ctcaagaaaa aagcgctaga acgagctaat
     1321 agacaatcaa aagcaccatt ttaagaggtg gaaaattgaa aatcgattat ctagaactaa
     1381 ttaatgaaat agcaaagtat aaaactggtg aggaaataga aatcctgaga gacgtatatg
     1441 atcaactcga agaagctgga atcgaaggaa ttaataatga tcgttcgagt tggagtaaac
     1501 tcagatacta tttcgcactc tatatcgata caacacaatt aagaaattta gcttatacaa
     1561 aattactatt tgttgattgc gttaaaggat tgcaaaaaca tcttagtgaa cttgagcagg
     1621 tgtaatcaga tggatctaaa aacatttaca gcacagatcg aactaatgca tcaagaagct
     1681 ttaagacaaa gcgcctcgta cgaagacaag tggctcaaca cgttccatgg tggacgtgag
     1741 agcgcacttg atcaagtact caaattattg aaaggggaac gtcgggatgg ataagaaagc
     1801 ggcaatgaaa cgaatcatcg aactgacaca ttctgagaat tggcaagaag acaaagaaat
     1861 agttacagaa gtccaaaagc tcggtaaatc aatgtggact gaaaagccca aacggagaac
     1921 gccgagaaag attgcaattt ggcatggtga tcgaattcta gtaacaggta ctgctgaaca
     1981 gttatctgaa attactggtc tgagcaaaaa catcatctgg gatagagcaa ggagcttatg
     2041 gattgattca aaaggacgac aatttaggta tgtggaggag aaaaaatgaa cgaactaatc
     2101 acaaaagtag agcagtgggc taaagacaag ggactggatc aagcagatcc aaaagcacag
     2161 tttttgaaag tagctgagga attcggggaa atcgcttcag caatggcaag aagtaatgat
     2221 gagctattta aagatagcgt aggagacgta atcgtcacgt tgattatcct ttccatgcaa
     2281 aaagggacaa acgtacaaga gtgtttagaa atggcataca acgaaatcaa agggcgcaca
     2341 ggaaaaatgg tagatggtgt attcgtgaag tcgagtgatt tggaggacag caaatgatag
     2401 gtttaacaat attttggtgt gcgattggtg gtttactagc atttctagga tactacctca
     2461 tctgtgaaag tagaaaagga agaaaatata cagtgacagg tattgtcctg ttaattatcg
     2521 gaattctagt tgcagttgga gcagaactga atctttatgt aaatggtaca caagaagatt
     2581 tggtgcgatt tatgttttgg atgaagaatt aggaggaaaa aaatgaacga aaacaaatta
     2641 atcaaattag gtgtagcagg agcagtaatc gtaggtattg gagttatcgg aggatttaag
     2701 ttcttcgaaa aaatcgataa tggatatgtg ggtgtgcgct attcaatgaa cggcggtatc
     2761 aaagatgaag cactgacgca aggtgtgaaa tttgtaggga ttgacaaagt gatccaatat
     2821 ccaattcgct tgcaaactat ccaatcaaaa aatatttcag tatctacaag cgacggcaaa
     2881 aagacaacga ttgatatcaa atatgactac aaagttgatt caactaaagc agcaaaaatg
     2941 tacaaggaat ttgggaatat cacttcggaa gatatcgaaa gtggatggtt aaaatctaag
     3001 cttcaaaagg tcgctcgtga agtttatgcg aaatatagtc tgcttgatgt cctttcagga
     3061 gattcctcta aagttgaagc tgaggtatta acgaactttg ctaaatcagt tgaatctaaa
     3121 gggtttgaag tcgaagacgt aacacttggt gttccagatg tcgataaaga aacacaaaaa
     3181 tcaatcgatg cgatcattcg agctggtcaa gaaaatgaaa aagcgaagct agatgcagaa
     3241 actgcaaaaa ctcaagctga tagtgaagct tacaagaaaa caaaagctgc agaagcagag
     3301 gcagaatcta atcgcaaagt cgccgaatca gtaacagaca atttgattcg ttatgaagaa
     3361 gctcaagctc gcaaaaagca tggatgggta acagtaaatg gagcagatac tgtagttacg
     3421 gatgaagcag gcaaataata tgggattctt tatggctaaa attctcttgt tcttaggttt
     3481 agtaggagca gcatatctcg tgtatgcgct cctttcccaa actgatgaca aagaagatga
     3541 caacaacgat gaaatgaaat tttaggagga gaaataatgg atctcattac acaatacagt
     3601 gatatcatcc tcaagaaaat catggcaaag attcagaaag ataaaaaagc aaaagaacga
     3661 gcggaattag ttaagttgga aatggctgaa acaggagtag gagtgcgaag tagcaggcat
     3721 tggaaagcag cagcaaacat tgaattttat tacaacgaaa ttcaaaaagg gttcgatcag
     3781 atgcgtgagc tggatcggca aacaaattgg agcaagaaac ttcatcaaga tcgtttcaaa
     3841 tttgtagaga agtataaaga aatattagaa gagtatttga ggaggacagc gaatgataaa
     3901 aaagctcatt caattcagca tggatttata tgatatcgaa tcaggagcaa cagtatctgt
     3961 ggagtctgat catttaatca taagttttgc tgacgaacgt caaattatca tatgggtagt
     4021 tgacgatatg ctatatccag aaattgtaca tgattttgaa gaatcaaaag cggttgagtt
     4081 tgaaatagtg aaaaaagtaa tggaattgat tgaaaaatac gaggaggaca gcaaatgata
     4141 ccgaagttta gagcgtggga taagcgagaa aacacaatga gagatgtagc tgtcttacat
     4201 tttactaaaa acggcaaaac aaactttatt gaatattgga taaatcctac cgaattgaaa
     4261 tcatatcacg tgcgaaacat cgacctcatg caatccacag gaatgaaaga taagaatggt
     4321 gtggagatat ttgaagggga tgtagtaatt acatcaaaag gagcgattgg atatgttgcg
     4381 tatcttcaac aagaagccgg ctttgtagtt gtactaaaga aaagtgatta tagattaggc
     4441 cataggaata ctggcgaaag ttatgatgta gcaactgctc atgaagtcat cggaaatata
     4501 tacgagaata gcgagttatt ggaggtagag tgattatctt agctactgac tacatcgatt
     4561 ctttaaaaga tgaaaatggg aatgtcccag cattgctaaa ttgtggaatt aaaagcaaga
     4621 atagaatcat tttgttacgg atatcggctg atttagcaaa atatctttat ctgagcaaga
     4681 cagcagtata ttacactgcg gctacacgtt atcagtacaa gggaaaaaca atcagtcagg
     4741 attattatga tcgtttaatc agtcctgaca tgcatggaaa atcaaaatca gcgatcaagt
     4801 tatttggcgc aatagagata tatgccgatg attttccaaa tttatggttg aaggaggaag
     4861 cggaatgaaa tacgaaatac cactaagtga agcgggcatt caagcaatta tcaatggtcg
     4921 ggaggttaat aaaaaacttc ctgatggtac tgaattagtc atcagacaaa gttatttgaa
     4981 agatatggca gctccagtat taattgatcg ttttaacgtg actgattctg tggtagagaa
     5041 ccacttgaaa gaatttcgat caagtataaa cgacactttc agattaggga gttgattgac
     5101 aatgaacacc agacaccgca gagtagcaaa actaagaaaa caggaactga atgtactaaa
     5161 gacaaagttt gaaaaagaat atggaatttc agcagaagaa acatataaag tggcaagtca
     5221 gtttgttgct gatgcgagtg agactattcg taagtttggg atttcgatat taaatgatga
     5281 tcgtaaatgg gaggcagaaa gatgaaacta aaagacggat tttacactga tgcttatgga
     5341 attggtggtt tgatgatgga tctaccaact aagaatccta taaagcaaga ggaatcagaa
     5401 attaaagtcg gtgacatggt tcgctgcgaa gcagaagggt tcatctatcc atttcgtgga
     5461 tatgtagagc atctctataa tcactcagcg atcattcgta ttgaaaacac aatggaatgc
     5521 gataagtggt tagcgaaaag caaagagaat ttagcagtgg tgagattggt ggatatggag
     5581 gttataaaca atggaattaa aaattgacaa ggtaacgttt gaagaaccag catttcaaga
     5641 aatggatgaa cgaattgcag aattggtcga gaaaaaacaa gatgaattga ttgctttgct
     5701 agaaaagaaa taccaatttc cgaaatatat gaacaaaatg caagcagcaa aatatcttgg
     5761 tgtaagttac aacacgatga tggataagta cgtaccagct ggtttgaaaa tgggtatcgt
     5821 taacggcgtt attcgtatca gcaaagaaga atgtgatcgt tttatgaaag aacatcaaaa
     5881 ataaaataaa aaaacgttta gccccttagc taaacgcaca attataattt acctgacagt
     5941 taattataac aaaattgcgg aggttgagga atggcggaat gaaaaagaca ataaaaacag
     6001 aagaagcata tattgaagta tttgacacac cagcatgcta agtcgagaag tgtaactcgg
     6061 caaataaaaa aagccggatc gctccgactg atgtaataaa cccaacaagt ttattatatc
     6121 acataaagga gcggtttgac ttgatgcaat tgttacgaga ggtagatttc aaacagacaa
     6181 gatgcaatgc gagagatgtg ctgaagaatt ttcgacgctt agagcggatg gcaggtcgct
     6241 ctttgataga tattaagtcg ccgattatta cggatatgcc gaaggcaccg aagcacggca
     6301 ataaggcaga ggacgcgatt attcagatga tggatataga agcagagaga gatgcgattc
     6361 tagcggcttt gatggctctt agtctgatta gccgtcagat actctattat agcttttgtg
     6421 acgtaaacaa gcactctaat tatgaaatag ggcaattgat acgaggatac ggagagaaga
     6481 atgtagagaa gctgaaatct atcgcgctga tcgaatttgc tgaagcatac aaaaaaggcg
     6541 tattagttca gtatcgttga ttttgtaggg tttttgtagg gatagtgtag ggtttttgag
//
LOCUS       DAZUKZ010000126         6570 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000126 DAZUKZ010000000
VERSION     DAZUKZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.130"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACG8EF_002472"
     CDS             127..579
                     /locus_tag="ACG8EF_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947735.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACG8EF_002473"
     CDS             593..1282
                     /locus_tag="ACG8EF_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947736.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACG8EF_002474"
     CDS             1407..3758
                     /locus_tag="ACG8EF_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFS4947737.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACG8EF_002475"
     CDS             3838..4233
                     /locus_tag="ACG8EF_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947738.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="ACG8EF_002476"
     CDS             4558..5175
                     /locus_tag="ACG8EF_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4947739.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="ACG8EF_002477"
     CDS             5216..5515
                     /locus_tag="ACG8EF_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFS4947740.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="ACG8EF_002478"
     CDS             5549..5716
                     /locus_tag="ACG8EF_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4947741.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="ACG8EF_002479"
     CDS             complement(5761..6366)
                     /locus_tag="ACG8EF_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4947742.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2110 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt
//
LOCUS       DAZUKZ010000127         6303 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000127 DAZUKZ010000000
VERSION     DAZUKZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.131"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            334..714
                     /locus_tag="ACG8EF_002480"
     CDS             334..714
                     /locus_tag="ACG8EF_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS4947743.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="ACG8EF_002481"
     CDS             696..1610
                     /locus_tag="ACG8EF_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4947744.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="ACG8EF_002482"
     CDS             1855..2544
                     /locus_tag="ACG8EF_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947745.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="ACG8EF_002483"
     CDS             complement(2665..2751)
                     /locus_tag="ACG8EF_002483"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS4947746.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="ACG8EF_002484"
     CDS             3427..4377
                     /locus_tag="ACG8EF_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4947747.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="ACG8EF_002485"
     CDS             4398..4931
                     /locus_tag="ACG8EF_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS4947748.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="ACG8EF_002486"
     CDS             4947..5117
                     /locus_tag="ACG8EF_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947749.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="ACG8EF_002487"
     CDS             5567..6100
                     /locus_tag="ACG8EF_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947750.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            6112..>6303
                     /locus_tag="ACG8EF_002488"
     CDS             6112..>6303
                     /locus_tag="ACG8EF_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947751.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKS"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DAZUKZ010000128         6173 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000128 DAZUKZ010000000
VERSION     DAZUKZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.133"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACG8EF_002489"
     CDS             725..811
                     /locus_tag="ACG8EF_002489"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS4947752.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACG8EF_002490"
     CDS             complement(932..1621)
                     /locus_tag="ACG8EF_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947753.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="ACG8EF_002491"
     CDS             complement(1628..1906)
                     /locus_tag="ACG8EF_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947754.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="ACG8EF_002492"
     CDS             complement(1866..2780)
                     /locus_tag="ACG8EF_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4947755.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACG8EF_002493"
     CDS             complement(2762..3142)
                     /locus_tag="ACG8EF_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS4947756.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACG8EF_002494"
     CDS             complement(3592..3879)
                     /locus_tag="ACG8EF_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4947757.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACG8EF_002495"
     CDS             complement(3899..4123)
                     /locus_tag="ACG8EF_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS4947758.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACG8EF_002496"
     CDS             complement(4578..4748)
                     /locus_tag="ACG8EF_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947759.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACG8EF_002497"
     CDS             complement(4763..5296)
                     /locus_tag="ACG8EF_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS4947760.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACG8EF_002498"
     CDS             complement(5317..>6173)
                     /locus_tag="ACG8EF_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4947761.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAZUKZ010000129         6167 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000129 DAZUKZ010000000
VERSION     DAZUKZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.134"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..2195
                     /locus_tag="ACG8EF_002499"
     CDS             42..2195
                     /locus_tag="ACG8EF_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFS4947762.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2164..3579
                     /locus_tag="ACG8EF_002500"
     CDS             2164..3579
                     /locus_tag="ACG8EF_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFS4947763.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3612..4646
                     /gene="sppA"
                     /locus_tag="ACG8EF_002501"
     CDS             3612..4646
                     /gene="sppA"
                     /locus_tag="ACG8EF_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS4947764.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4648..5169
                     /locus_tag="ACG8EF_002502"
     CDS             4648..5169
                     /locus_tag="ACG8EF_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS4947765.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            5166..5552
                     /locus_tag="ACG8EF_002503"
     CDS             5166..5552
                     /locus_tag="ACG8EF_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS4947766.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         2194 a          848 c         1152 g         1973 t
ORIGIN      
        1 tttttctata aaaatttttt agaaggagtt gtgttagata agtgataaaa tcatttcctt
       61 ttgtgcagca acaagatgaa aaagattgtg gtgttgcttg cctttcgatg attttaaaat
      121 attttaaaac tgatatcccg attcataaat tacgggacct atctgggact gatttagagg
      181 gtacttcagc atttggattg aaaaagacat ttgaaaaatt gtcttttgat tgtatggcta
      241 ttcaagcaga tagtgagatc tggaaagaaa gagatcttat cttaccatta attgcacata
      301 tattggttga taacagctat atgcactacg ttgtaattta taaaacgaag ggaaacgtac
      361 tatggattgc agatcccgct aatggaaaaa atagaaaaac gattgaagcg tttcaaaaag
      421 aatggacagg tattctttta ttacctatgc ctagagaaac ttatattcct attaaagaaa
      481 aagtagctgg attaagttct tttttgccta taatttggaa acaaaaagga ttagtattcc
      541 acatcgtttt agcatcgatt ttcattacgt tttttggcat aggaagctca tattattttc
      601 aaggtattct tgattttttc atccctaatc aagcacgttc aactttaaat ataatttcca
      661 ttggcttgat cgctgtctat ttatttcggg tattgtttga atatagtcgt agttatcttc
      721 ttattatttt aggacagcga atgagtatgg caattatgct tcagtatttc aaacatgtgt
      781 taactttacc aatgaatttt tttgctacga gaaaatcagg ggaaattatt tctcgttttc
      841 ttgatgccag taagattatt gatgctttag caagtgctac gttatcaata tttttagata
      901 ttggtatggt gttgcttgtt ggggttacgt tagctattca gaatagtacg ttgtttttaa
      961 tcactctatg ctctttgcct ttttatgtag ttgcaatatt cgctttcgtg aaaagctatg
     1021 aaaaatcgaa cagggaagaa atggcagccg gtgcagctgt aaattctagt atcatagaaa
     1081 gtcttaaagg aatagaaaca attaaagcat atagcggtga agagaaagta tataaccgta
     1141 tagaccatga atttgttaaa ttaatgaaaa aatcatttcg tacggttacg cttgataata
     1201 ttcaacaagg aattaaacat ttgattcaat taattagcag tgcattaatt ttatggctag
     1261 gttcttattt tgttatggat aatagaatta gtctaggtca acttatcaca tacaatgctt
     1321 tactagtatt tttgacagat cctttacaaa acattattaa tttacaagtg aaaatgcaaa
     1381 cggctcaagt tgcaaataat cgtttaaatg aaattttcgc gattgattca gaaaaatcga
     1441 accagtcttt tgaatatatg ctttctgaac aattatttca acaagatatc actgtaaaaa
     1501 acgtttcatt ctcgtatagc atgaaggccc caacgttgaa cgaaatttca tgtacaattc
     1561 cttcgcacag taaaacggct atagttggag ttagtggttc tggaaaatca actcttgcaa
     1621 agttatttgt tcgtttttat gaagcatcag aaggatcgat ccattatgga aacattaatc
     1681 acttagacat tccttttcag atattacgcg atcgagtgac atatttgcct caagagtcat
     1741 ttttctttag tggaacaatc attgagaatc ttatattcgg tttagatagc ccaccaagtt
     1801 tcgaacaaat aatagaagtt tgtgagatag tccaactaaa tgaatttatt aatcaacagc
     1861 cattacgtta cgagacattg ttggaagaag gtggaaataa tctttcagga gggcaacgac
     1921 aacgattagc cttagcacgg gctttattaa aaaatccgga catattaatt ttagatgaag
     1981 caactagtgg attagatacc ttgttagaac atgcaattat gaattatctc ctcaatttaa
     2041 aagaaaaaac gattattttt attgcgcatc atttaccaat agcaaaagca tgtgatcaaa
     2101 ttttagttct ccatgaaggg catttggtag aaaaaggaac gcacgatgaa cttcgttttg
     2161 ccgatggtat ctatcaaaaa ttatgggaaa tctaggagag ggaaacaatt tatgcagaat
     2221 aataattggt tcgaacattc tagtatctat actaaacaac atcatagctt ctaccgttgg
     2281 gtgttatacc ctgtaattct ttttttatgt attattggat tgtttttaac ctttgctaaa
     2341 aaagaggttg ttattcggac aacggctcaa ttgaaccccg aaaaaataga gaagttacaa
     2401 gtgccattag aagctaaaat aatggaaaac ttcctaaaag aaaatcaatt tgttcataag
     2461 gacgatgtgt tggttagatt ggattgttct ttaatagaga atgaaaaggc tcagatagaa
     2521 caagaaaacc aacgtattac tcaacaaata aagatggctc agctatttat tgaaagtata
     2581 agtaaaggaa aaaatttgtt ttcaacggat gacagttttg gctacagtaa tcaattaaag
     2641 agcatgttgt cagaaaaaga atcactccgc tacgctttga agcaaagtga attaaatgat
     2701 caaaagcaat tagaagtata cgaaaagaca aaaagacaac tagaaaaaca aattgagagt
     2761 tcagatagta aattacaaga atggcaacaa gtacaggtag cttggagtaa taatcaatca
     2821 ttaaaagatt tttcaaaaga aatgatggca aactatgaga attggcaaga acaactaaat
     2881 aatgtttctg atgatcaaaa aaatcaagtg aaactgacaa tttcagcaag cataaatgaa
     2941 caaattgagc aactaaaaaa agaagtagaa cagtatcagt cagaaaaagc taaattagtt
     3001 aaaccaacta cttctgagaa tgacagaatt agtcaaacgg aaaaaggaaa gcaagagcta
     3061 gaacaagcaa taacaactgt aaaacaaaca gttacagagc taaatgaaaa acaagagaag
     3121 aaccagtcag ttatcaaaag tttggacgat caactttcaa aaggaatact aaaagctcct
     3181 gtatctggaa cggttcattt aaatgaagaa acaaagggac aagcagaact agccaaagga
     3241 acagtgcttg cagaaattta tccgatccac gaaaaatcaa aaatgaaatt tacagcactt
     3301 ttacctgcga atgaaagtac ctatattaaa gaagggatga aggttcattt taaattggat
     3361 caaaaaggtg gggctccgat tacgatcgac ggaactttag atgaaatttc agaaaattct
     3421 actgccactg agaaaggtgt tttttatgca gtgaaaggaa cgttaaaaac aactcaatcg
     3481 tttccgtttc gttatggatt aactggagag ttgtctttaa ttgtaggaga aaaaacatat
     3541 tggcaacaaa ttgtcgatgt gtttctaaat aaaaaataat tatttttact aagaatatag
     3601 gaggagttta tatgaataaa agaagatgga ttgctgtggg agtagcgtgc ggattattgt
     3661 tactatccat tatcgtcgcg ctaattccag gaaaagataa agaggaagca tcaaatacaa
     3721 ccctaacagg aataaataag attttttacg gatctaacga aatcacagaa gaaacgttag
     3781 aagaaggcgc atcaaataaa aagattgtca aactatccgt caatggagtt attgcagata
     3841 caggtgaaag caatcttttt agtagggaac aatataacca tcagaatttt ttaacacagc
     3901 taaagaaaat tcaagaagac aaagcggtta agggagtgtt attagaagta aattcacctg
     3961 ggggaggaat atatgaaagt gctgagattg ctaaagaaat ggcaaaaatt aaaaagctag
     4021 atattcctat ctatactgca tttaaaaata cagcggcaag tggagggtac tacatttctg
     4081 ctggttcaga taaaatattt gctacggaag aaacaacaac tggatcaatt ggtgtcatta
     4141 tttctggttt aaattattct ggcttattag aaaaacttgg cgtaactgat gcgacgtata
     4201 aaagtggtgc attaaaagat atgatgtcac ctcaacacaa accttcagag gaagagaata
     4261 aagttataca ggaatttgtg atgagtgctt atgatcgttt tgtcaatgtt gtagcaaaag
     4321 gaagaaatat ggatactaat gcagtgaaag aacttgcgga tggaagaatt tatgatggta
     4381 accaagcagt agaaaatggg ttggtggatc aaattggtta ctctgaagat gcattagatt
     4441 ccctaaaaaa agagaagaaa ttaactgatg cgacggttat agaatataaa aatgacacaa
     4501 ctggctttgc ttcttcttgg ttaggtaata agattgctga atggcaaggg ctaaaggcaa
     4561 ctacatcaga tcgtgtaatt tcagtttttg aaaaacttgg attttctgat actccaaaac
     4621 caatgtatta ctatggaggg ctataaaatg aataagaaga gtggcattga aactttgcct
     4681 aattatgtat ttgcgggatt ttggttacga ttttttgctt ttcttattga tttaatttgt
     4741 attagcatca tcacccatgc tactataggt ctcttttaca aaatcatggg actggaatct
     4801 gacaaatcca ttattacttt ttatggtata gcgtcattag ctatttattt aacctatttt
     4861 gtacttttga ctaagctgaa ttcaggacaa acaattggga aaatgatttt tgggataaga
     4921 gtagtgagct ttgaggaaaa agagctaagt tggcaaacta tctttattcg tgagtgtata
     4981 tgtaggttta tattaaaaaa ttttgtattt caaattgggt atttggtggc tgctttcacg
     5041 cccaagaaac aacatattgg ggattatttt tctaacacgt cagtggttgt tattcgttca
     5101 ttagaagcta ttaactatgg tcaaaaaatt tctaattcta aaacacttaa tctggagaga
     5161 attaaatgaa taagagaaaa acagagttta ttttgatttt aattagtgga ataacaggga
     5221 ttttatgtag tcttttagca ttgatgggtg gtatgtttct atcttctatt gattcaacta
     5281 ctttaaatac tacagaacta accagtaagg atatagatat gttagtcaat ctaagcggga
     5341 atgttgtttt atttggagga attaccctag ttttttcgat aatgattttc ataataggtt
     5401 ttttagtaag aaaaaatcaa aaaacagtgg tctatggtat tagtttactc attttatcta
     5461 ttattccatt ttttctttta acattattgt gggtaattcc tggagtttta ggtattattg
     5521 cgggattgat gcttctaata agaaaatctt aattaggcaa tgtgtagaca acgtgaaacg
     5581 attaatcaat taaaagctaa atacgttttg agaaatgata acttgtagtc gatggaagac
     5641 atgtagataa aaaaactacg tgtcttttat ttattctaat tttttcgaaa aaaagacact
     5701 ctctgattgt tgtattaatg gaaaagggga aagcagcaag taaatttagt ttttctatta
     5761 aatattcttc actttttaac cttttatagt acttagattt aatcctatat gtttagagag
     5821 ttcattggca gcatgatttt gcaaagcttc tcgaattatt gttatattag aaactttact
     5881 gttcataatt atgcttcttt tttaactcaa agtccaaagg gctacagtgg actttgagtt
     5941 aaaaataaaa agccctagtt attttgaaaa ataaaaaaag aacagcgtaa atgttttcga
     6001 aatgctttcg caccaacatt tacctgttcc gtgggtttgg cacccaatgg gccgtgaggg
     6061 gctcgaaccc gcgacccgct gattaagagt cattaatgtc aatttttaaa taaaattaat
     6121 atgtctcttt aagtattaaa ctatcactct ttatcctatg ataaatc
//
LOCUS       DAZUKZ010000130         6111 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000130 DAZUKZ010000000
VERSION     DAZUKZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.135"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..221
                     /gene="atpE"
                     /locus_tag="ACG8EF_002504"
     CDS             6..221
                     /gene="atpE"
                     /locus_tag="ACG8EF_002504"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFS4947767.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            312..836
                     /gene="atpF"
                     /locus_tag="ACG8EF_002505"
     CDS             312..836
                     /gene="atpF"
                     /locus_tag="ACG8EF_002505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFS4947768.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            823..1365
                     /locus_tag="ACG8EF_002506"
     CDS             823..1365
                     /locus_tag="ACG8EF_002506"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFS4947769.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            1388..2944
                     /gene="atpA"
                     /locus_tag="ACG8EF_002507"
     CDS             1388..2944
                     /gene="atpA"
                     /locus_tag="ACG8EF_002507"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFS4947770.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            2958..3860
                     /locus_tag="ACG8EF_002508"
     CDS             2958..3860
                     /locus_tag="ACG8EF_002508"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFS4947771.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            3883..5289
                     /gene="atpD"
                     /locus_tag="ACG8EF_002509"
     CDS             3883..5289
                     /gene="atpD"
                     /locus_tag="ACG8EF_002509"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFS4947772.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            5303..5725
                     /locus_tag="ACG8EF_002510"
     CDS             5303..5725
                     /locus_tag="ACG8EF_002510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFS4947773.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         1996 a         1132 c         1350 g         1633 t
ORIGIN      
        1 caattatgaa ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt
       61 atggtaacgg tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt
      121 ctggtcaatt ccgtacaaca atgtttatcg gggtagctct agtcgaagct gttcctatct
      181 taggggtagt tgtcgccttg attctagttt ttggtgttta attgaatcag tattaaggca
      241 gaaacaattt ggtttgttac tgctttttta catgaaaact agaagtactt caaagaaagg
      301 aaggctcgcc tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggaa
      361 atgtcatcgt tgtcagcggc tcatttttga tactactggc tctcttgaag cattttgctt
      421 ggggaccaat cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttggatt
      481 ctgcagaaca atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt
      541 tagcctctcg ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa
      601 gccgccaaaa tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agtaaagccc
      661 aagctgatat cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gatgttgcag
      721 aactatctct tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcacg
      781 aatcattaat caatcaatat atcgaaggtc taggttcttc aaatgaaact agataaatat
      841 acagtaggaa gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaataaagca
      901 gaagaaattt atcaagatct cgtaagctta cgtcaaatct atgaacaagt acctggatta
      961 ggtaatatgt taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag
     1021 cttgttagtg gctatgatgg aatcgttaaa aactttttag aagttgttta tgcgtataac
     1081 cgaatggatg atcttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa
     1141 ggtttgctgt tgggaagtgt cacaacagca gttccacttt cagacgaaca acgaatacaa
     1201 ttagaaaaga atgttgcaaa aacaatgaac tatcaatcag tcgaattaaa acaaatcgtt
     1261 gattcttcta ttattggtgg cgcgattgtt gaagctaacc atcgagtaat cgacggaagc
     1321 attcgcacgc aattagaaaa tatgcgtaat caattaaata gatagtacaa agaggtaggt
     1381 gaatcgaatg gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa
     1441 ttatcaaaac gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat
     1501 cgctcgtgca catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaatgg
     1561 ctcatacgga atggcgcaaa acttagaatc aaatgatgta ggtatcatta tccttggcga
     1621 tttcgagtcc attcgtgaag gagataaagt aaaacgaaca ggaaagatca tggaagttcc
     1681 agttggagag gccttgattg gacgggtagt taatccgcta ggtcagccaa ttgacggact
     1741 aggtgaaatc gttactgaca aagctcgtcc agtagaagca atggcaccag gcgttatgca
     1801 acgtaaatct gttaacgaac caatgcaaac aggtctaaaa gcaatcgacg ctcttgtacc
     1861 aatcggacgc ggccaacgtg aattagttat cggtgaccgt aaaacaggga aaacttccat
     1921 tgcgatcgat acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat
     1981 cggacaaaaa gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat
     2041 ggattacaca atcgttgtga atgccggtgc gtctcaacca gcgccgttgc tttatatcgc
     2101 accatatgct ggtactgcaa tgggtgaaga gttcatgtac aacgggaaac atgtcttgat
     2161 catttttgat gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg
     2221 ccgtccacca ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt
     2281 ggaacgtgca gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt
     2341 tgttgaaaca caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac
     2401 tgatggacaa atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga
     2461 tgccggacta tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt
     2521 tgctgggact ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt
     2581 cggttctgac ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga
     2641 aatcttgaaa caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta
     2701 tgctttgaca catggattct tagatagtat ccctgtagac agtattttag acttcgaaca
     2761 tgaattattc gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac
     2821 aaaagatctt ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt
     2881 cttagctaca aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc
     2941 atagtgaggt gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa
     3001 cgaaaaaaac gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa
     3061 aatctgaagc ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta
     3121 cgcatttgac tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca
     3181 attacaacag catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg
     3241 cagatggcgg tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt
     3301 tagaagaaga ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg
     3361 cagacttctt taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc
     3421 agccaagctt tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg
     3481 aagtattcga tgaattatac gtatgttata accatcatat caattcgcta actagtcagt
     3541 ttcgtgtaga aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg
     3601 aacaagaata tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt
     3661 atgctgaaag tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg
     3721 gtatgacagc aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg
     3781 tttcttacaa ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg
     3841 gagcttcggc actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt
     3901 gttcaagtaa tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat
     3961 atcaacaacg cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa
     4021 gcagcgttag aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt
     4081 ttacaaagag gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa
     4141 gaaacattag gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc
     4201 ccagaagatg cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt
     4261 acaagtacag aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta
     4321 aaaggtggga aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa
     4381 gaactgatcc ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt
     4441 gaacgtactc gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa
     4501 aaaacagcca tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct
     4561 ttgactggtt tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg
     4621 tttatcgaca acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga
     4681 cggatgcctt ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa
     4741 gaacgtatca catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca
     4801 gccgatgact acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc
     4861 aacttggaac gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct
     4921 tcttcaagtg ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa
     4981 gtccaacacg tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg
     5041 gatgaattat ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc
     5101 ttgtcacaaa actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct
     5161 gtagaagaaa cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct
     5221 gaagaagctt tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta
     5281 ggctactaga aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc
     5341 cggtttggtc tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct
     5401 tgggattttg cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt
     5461 aaaacgaaca gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga
     5521 agttcgtgat aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga
     5581 tgtatctcgt gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc
     5641 aaaacaagat acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg
     5701 tatcaaagta tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca
     5761 gtaaaaagag gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa
     5821 ttgcttctca aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca
     5881 ccgtatatcg gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa
     5941 aatagttcct catattttgc cgttttttga gctaataccg agggtcagat gattgaacac
     6001 cgtttatttg gaataaggga ctgcgacaag acttttgggc tctttgtcaa taaggactga
     6061 tgagttgtgc aaaatcaaat ctgagtcaga atgaaccaca ttctggctca g
//
LOCUS       DAZUKZ010000131         6002 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000131 DAZUKZ010000000
VERSION     DAZUKZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.136"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACG8EF_002511"
     CDS             <1..395
                     /locus_tag="ACG8EF_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS4947774.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="ACG8EF_002512"
     CDS             398..1228
                     /locus_tag="ACG8EF_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFS4947775.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="ACG8EF_002513"
     CDS             1244..2095
                     /locus_tag="ACG8EF_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4947776.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="ACG8EF_002514"
     CDS             2092..2451
                     /locus_tag="ACG8EF_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFS4947777.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="ACG8EF_002515"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="ACG8EF_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="ACG8EF_002516"
     CDS             2624..2929
                     /locus_tag="ACG8EF_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947778.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="ACG8EF_002517"
     CDS             2929..3240
                     /locus_tag="ACG8EF_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFS4947779.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="ACG8EF_002518"
     CDS             3237..3410
                     /locus_tag="ACG8EF_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947780.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="ACG8EF_002519"
     CDS             3407..3823
                     /locus_tag="ACG8EF_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFS4947781.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="ACG8EF_002520"
     CDS             3823..4491
                     /locus_tag="ACG8EF_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFS4947782.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="ACG8EF_002521"
     CDS             4488..4865
                     /locus_tag="ACG8EF_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947783.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="ACG8EF_002522"
     CDS             4862..5161
                     /locus_tag="ACG8EF_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947784.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="ACG8EF_002523"
     CDS             5151..5309
                     /locus_tag="ACG8EF_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947785.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="ACG8EF_002524"
     CDS             5403..5819
                     /locus_tag="ACG8EF_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS4947786.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DAZUKZ010000132         5907 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000132 DAZUKZ010000000
VERSION     DAZUKZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.137"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(119..604)
                     /locus_tag="ACG8EF_002525"
     CDS             complement(119..604)
                     /locus_tag="ACG8EF_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947787.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(743..958)
                     /locus_tag="ACG8EF_002526"
     CDS             complement(743..958)
                     /locus_tag="ACG8EF_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFS4947788.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1182..1982
                     /locus_tag="ACG8EF_002527"
     CDS             1182..1982
                     /locus_tag="ACG8EF_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4947789.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2001..3869
                     /locus_tag="ACG8EF_002528"
     CDS             2001..3869
                     /locus_tag="ACG8EF_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS4947790.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3862..4353
                     /locus_tag="ACG8EF_002529"
     CDS             3862..4353
                     /locus_tag="ACG8EF_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFS4947791.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4341..4895
                     /locus_tag="ACG8EF_002530"
     CDS             4341..4895
                     /locus_tag="ACG8EF_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFS4947792.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4913..>5907
                     /locus_tag="ACG8EF_002531"
     CDS             4913..>5907
                     /locus_tag="ACG8EF_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFS4947793.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2063 a          945 c         1191 g         1708 t
ORIGIN      
        1 agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat aactttctca
       61 gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata tcatctaatc
      121 aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac actcgataaa
      181 aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct gtgaacaaat
      241 aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt aattcggaac
      301 ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga gaaggtacag
      361 tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt gattggctat
      421 gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa gtacttttag
      481 ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa ttctttgttt
      541 tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa atccctttat
      601 gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta aacaaagaaa
      661 agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg aagataataa
      721 cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta atcattggga
      781 aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc ttcgcaaaaa
      841 tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag cctaccaaca
      901 gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaaa gttttcatta
      961 gaattctcct ttcttaaatg ataaaacaat tatatatcaa aaagagaaac attgcttata
     1021 taacgctcag gaattttagc aatctatgca aaaagatgaa aatttttttt gctcttccat
     1081 tagtacacat tttttagctg aagctgtaaa tttcatgtct taattttttt tagtgagctt
     1141 ttatactgga tttgtaagaa aaaagaagga gaggatcaca tatgtcggat tggttggata
     1201 ttgcaggaaa agtagtaatt gtaactggag gttcctcagg aattggaaga agtatcgtcg
     1261 aaaatttgtt gaagcagaat gcacaagtag cgaattttga tgtaactgaa tgccgtatac
     1321 agcatgaaaa cctactatct ttaaaagtcg atgtgtcttc taaaacggac ataaaagaag
     1381 ggatatataa agtaatgaaa cattttaaga cgatcgatgg tctagtcaat aatgcaggaa
     1441 taaatattcc ttctcttcta atagatagaa atcatccaaa aagcaagtat gaattatcag
     1501 aacaagtttt tgataagatg atcgctgtca atcaaaaaag tgtatatttg atgagccaag
     1561 cagttggaag aatcttggtg caaaagggat caggagtgat cgtgaatctt tcttctgaat
     1621 caggattgga aggatcagaa ggacagagct gttatgctgc tacaaaagca gcgatgaaca
     1681 gttttactcg ttcttgggca aaagagttag gaaagaggaa tgttcgtgtg gtaggtgttg
     1741 caccagggat actggaagaa accggtttga gaacacaaga atatgaagaa gctctttctt
     1801 atacgagagg tataagcgtc gaacagctga gaaacggtta cagtcgaaca agtacgatcc
     1861 ctctaggaag gagtggtaag ctgcaagaag tggcggatct agtctgctat tatctttcag
     1921 aaagatcaag ttatatcact ggagtcacga ctaatatttc aggaggtaaa acaagaggat
     1981 aaaggatagg ggggacgcac gtgacgctag taaacagatg gtaccaatta ttgaaattac
     2041 tagtcactca caagaaagtt tcaatccagg aagtgcaaaa agaaatcaaa accagtaatc
     2101 aaaccataaa aaaagatatt ggggagttga atgaagaaat tgccggtatt gcaaaaatca
     2161 tccaaaaaaa caaacatttt gaacttgaaa tttatgattt tgattttttt gatgagatca
     2221 tgcaaggaaa attgaaaaca gccagtgatt tcaattcggc atccaaaaga atcgcttatt
     2281 tactaaatag gctgatcgaa acagaggatt tcttacgtat ggatgatttg tctgaagaag
     2341 tcggtgtcag cagaggaaca gtggtcaagg atctaaagac attaaaagag atgatcaatg
     2401 attttgatgt aaagattaca ggcacaccaa ataaaggact aagaataata ggagatgagc
     2461 tagaattacg actactttta ttttattttg tttctcccta cttttcagag acgaaaacag
     2521 aacagtttct agaagaaaaa caagcaatca aacattttca gaagaagacg aatgctagta
     2581 aacaagaaat caccctttta acgaaagtta tcggtatttc tttgaataga atctctagta
     2641 actatctgtt gaagaagccc atcaatcgtt acagtgggtg ttttgaatat atccaagaat
     2701 tcaatgaact gatcaatcag ttggaagaaa tctatgaact aactttgagc caatatgaaa
     2761 aagattttat cagttttcct ttgaacatca tcaacacaag tataacgatg ataaggaaag
     2821 ctaactctga tttgcgctta tatttcgacc ccatgatgat gcgtattcga cgattagatc
     2881 tcattgatct ggatgagtcc ttcttatatc aagagatgaa acagcacctc tctttattga
     2941 taaatcgggt aatttttcgt tatcgattga ctgatttatt ttatggtgag atagaaaaaa
     3001 agtatccttt ttcttaccaa atcgctagcg aatgcataca agcattagaa gctatcttgc
     3061 aacataagat ctctaaagta gagatcagct acctaacgtt gtatttggag ttagcatttc
     3121 gatatcaaaa agagcatttt tcctctaaaa aagtagccat tgttcatacg acaggaaaag
     3181 gaacagcatt aatgatccaa agacaaatca aaaggatatt ggggagggat atacaaataa
     3241 cacatgtttc agaagaacaa tatgaaaaag tagatctaaa caaatatttc gctgttttta
     3301 caacgattcc gttaaaaaat gtaaatgaag agacaccggt gatccatttg actaatcttt
     3361 ttgatgactc ttggttaagg gcacaatggc aaaaagcaaa aaaaaagcga ttagccgggt
     3421 ttcattctac tgtttttttc tatcaaatat ggaaagaaaa aaagacgtat caagaatatt
     3481 taaacaaatt agtcgctcct ttgatcagag gctattatgt ggatgaagaa tttgaaaaaa
     3541 ggatcttgaa aagagaatat cagcaaagca catttgttaa caaagaattt gcttttcctc
     3601 acacaaccaa ccagctatct caacacattg ttttatcagt agggctaata actgatcaag
     3661 gtttagcaga aacaggagga aatggagtta tttttttatt tgccatacct gaagaaatga
     3721 cggctgaagt tgagaatgaa ttatttgagc tatacgatat cctatttgaa ctcataaaaa
     3781 tgggaataga tgagtttatt gaattagcac cgacacaaga atcattttta aaactaatca
     3841 aaagtaaagg agagagactt catgaatgat atgtatctat tcatagtttt cgcagcttca
     3901 gcttatgtgc tgcaagcagt tatcagttgg aagcaactaa agcatttcaa tcaaacatat
     3961 agcgaactgc gaagaaaagg aaaagtagcg attggtaaac gacctgggaa aatccgttca
     4021 ggcacgattg ttatgctagc tatagacgat catgatcaag ttttagaagt cagaaaaatg
     4081 caaggggtta catctttggc aaaatttcat tcattacctg tctatgcggg tgaagatatc
     4141 cattacatcg atcgttacca tccctcagtt cgagcggaag atcgtttgac acaagcagct
     4201 ttagaaaatg ccagagaagt atacttacga atagaggcag gaaatgaaaa aagtgaacaa
     4261 ccttcattgc cttttattgc tatgctcaat aattttcgga tatggaaaag tacgatcaaa
     4321 gaaaaaagga gtgacaaacg atggatatca taacaaaatt tgctgaggga tttatgaggc
     4381 tgttccaaac aggtgcagaa acattcataa gctggatgac aggaatcgta ccagttgtct
     4441 tgatgctgat ggttgcaatg aatacgctga ttgctttttt aggtgaagac cgagtaacga
     4501 aagtagccaa attatcagcg aaaaatccgc ttactcgtta ccttgtgtta ccgttcttat
     4561 ctgcttttat gttgggaaat cctatgtcat ttacaatggc tcgtttcctt ccagaatact
     4621 acaaaccaag ctattatgct gctcaagctc aattttgtca tacgagcaat ggtgtattcc
     4681 cccatattaa tcctggagaa ttgtttgtat ggatggggat tgctcaaggg atcgaaacct
     4741 tagggctaaa ttcgatggag ctggctatcc gatacttgct tgtgggactg ctgatgaact
     4801 ttattggtgg ttggataact gattttacta ctggatttgt atgcagacag caaggaatcg
     4861 ttcttagcaa aaaagtagag ctttcagttg attagagaaa ggagagtttt aaatgtatgg
     4921 tgatattaag atcgtaagag gaaacggcgg gtatggaggt cctttgatta ttaggccgac
     4981 aaaagataaa cacaagttca tctatgtcac aggtggaggt gagaggccaa tgatcgtaga
     5041 taaaatagaa gaactgacag gtatggagtc tatcaatggg ttcaaaacat caatacctga
     5101 tagtgaaatc gcgcttgcaa tcgttgactg tggagggact cttagatgtg gaatttatcc
     5161 taaaaaaggg ataccaacca tcaatatcgt tcccacagga aaaagtggcc cattagcaca
     5221 atatatcact gaagatattt atgtttcagc agtagggtta gaacaaatac agccactaat
     5281 ggaaactgat gataacaaag aaacagtaaa tactgcatct ccagataaaa agtacaaata
     5341 tgatacttct aaaaagataa ccgagcaaaa agcagagcag caaaactgga tagcaaaaat
     5401 tggaatgggc gcgggaaaag tagttgctac attcaatcaa tcagcaagag aagcagtaga
     5461 tacgatgatc cggacagtga tcccttttat ggctttcgtt tcccttttga ttggtgtgat
     5521 acaaggttct gggatcggta atgtatttgc taacattatg gcacctttag caggaaatgc
     5581 agttggatta acaatcattg gctttatttg ttcactgcca tttttatctc ctttgcttgg
     5641 tccaggggca gttattagcc aagtgattgg aacattgatc ggtgtagaga ttgggaaagg
     5701 aaatattcct ccccaattag cattaccggc actatttgct atcaatactc aaaatgcatg
     5761 tgattttatt ccagtcggtc taggtttgga agaagcaaaa gctgagacag tagaagtagg
     5821 tgtaccatca gttctttatt caagatttct taacggcatc ccacgagtta tcattgcttg
     5881 ggtagcaagt tttggattat atagctg
//
LOCUS       DAZUKZ010000133         5809 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000133 DAZUKZ010000000
VERSION     DAZUKZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.138"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            450..1604
                     /locus_tag="ACG8EF_002532"
     CDS             450..1604
                     /locus_tag="ACG8EF_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFS4947794.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1577..2020
                     /locus_tag="ACG8EF_002533"
     CDS             1577..2020
                     /locus_tag="ACG8EF_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947795.1"
                     /translation="MGQYHYKLVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="ACG8EF_002534"
     CDS             3143..4075
                     /locus_tag="ACG8EF_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4947796.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="ACG8EF_002535"
     CDS             4092..4607
                     /locus_tag="ACG8EF_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS4947797.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="ACG8EF_002536"
     CDS             4903..5184
                     /locus_tag="ACG8EF_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947798.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="ACG8EF_002537"
     CDS             5186..5602
                     /locus_tag="ACG8EF_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947799.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DAZUKZ010000134         5787 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000134 DAZUKZ010000000
VERSION     DAZUKZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.139"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..256
                     /locus_tag="ACG8EF_002538"
     CDS             <1..256
                     /locus_tag="ACG8EF_002538"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947800.1"
                     /translation="MSHMQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRN
                     NIKPFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            319..741
                     /locus_tag="ACG8EF_002539"
     CDS             319..741
                     /locus_tag="ACG8EF_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947801.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(835..1152)
                     /locus_tag="ACG8EF_002540"
     CDS             complement(835..1152)
                     /locus_tag="ACG8EF_002540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947802.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(1179..1385)
                     /locus_tag="ACG8EF_002541"
     CDS             complement(1179..1385)
                     /locus_tag="ACG8EF_002541"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947803.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(1366..1548)
                     /locus_tag="ACG8EF_002542"
     CDS             complement(1366..1548)
                     /locus_tag="ACG8EF_002542"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947804.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(1562..1912)
                     /locus_tag="ACG8EF_002543"
     CDS             complement(1562..1912)
                     /locus_tag="ACG8EF_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947805.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(1938..2240)
                     /locus_tag="ACG8EF_002544"
     CDS             complement(1938..2240)
                     /locus_tag="ACG8EF_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947806.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(2243..2476)
                     /locus_tag="ACG8EF_002545"
     CDS             complement(2243..2476)
                     /locus_tag="ACG8EF_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947807.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(3380..3910)
                     /locus_tag="ACG8EF_002546"
     CDS             complement(3380..3910)
                     /locus_tag="ACG8EF_002546"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947808.1"
                     /translation="MKMTPNHLRHICCEVTLFNRQRWKLSNRIVRRIIVDSTGKSTVI
                     LTKKLATKQDFQLFFSQVKTIFFDGKIIANRGCSNQDLMVSNIVFSRMKRRQDLLIQA
                     RKAYKVFKKQEKQSRGKQNEQIMNKKCIEVLNGKNDHSDTKKKEGRHENYPILLVCPN
                     CFSCGFVGRIGFRTWN"
     gene            complement(3907..5022)
                     /locus_tag="ACG8EF_002547"
     CDS             complement(3907..5022)
                     /locus_tag="ACG8EF_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS4947809.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(5028..5426)
                     /locus_tag="ACG8EF_002548"
     CDS             complement(5028..5426)
                     /locus_tag="ACG8EF_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS4947810.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         1690 a         1178 c          764 g         2155 t
ORIGIN      
        1 aatgtcacac atgcaactta ataatttaaa tgaaatacat gattgggctc aaaataattt
       61 acttaatcgt aaacaagctg ccaaaatcac tggacaaaac tatgatgctt tttctagctc
      121 tttacgtaga aataatatca aaccatttct agaaataggc gacgaacctg gtccttccat
      181 cattcgctta tatctaagag aagatattga aaaatacgca aaacagctcg aagaaaaaag
      241 acagaaacgt caatgaggcg ttttctgtct tggatttaca agtaaaacaa taataaacac
      301 ttttacaacg gaggttctat gaataagaaa tacattgttt cagttggttc aatcgtactg
      361 attgtactcg ctattattgc tttttctgta tccaacaaaa cagaaacaca gccacaaaat
      421 acggttcaat cgactactac aagtcaatct agcgatcatt ccgtctatcc ttcactaaat
      481 agtttaaaag aaacgcatgg acaagattta attgatcttg gttcttctca aattactact
      541 gtcttatttg aaaagaaaaa tattgcgttt attactccta aagaaggaac tgaccttgaa
      601 ttttctatca aagtaacaaa taccaaagaa aatttattct ccattcctga cattcaatat
      661 acccctaaca agttaaagat tggggatacg tttggtttgg ctaaagtaaa tgaacaatac
      721 tttgcttact cactgaaata agtttctaat agcaagagga aaaaccctct tgttattttt
      781 atcacataat cattttataa aaacgcttcg aaaatatcaa catttttgaa gaaactattt
      841 ttctaatttc tcaatttctt ccaagggatt ttcgctaatg acccatacag gcacagattg
      901 ctctcgaggc tccccttctt ctaaccacca acctgcataa tgaacttctt cctcctcagc
      961 attaccatga taccaaccaa caaaggattc atctacaacg aatgaaattt cttcttctgg
     1021 atcaaataat tcatttttcc atgcttcaat tgcttgttct ttggtgtact tttgtttgtt
     1081 aaatacacca caaatttcgt catctacata aaaaatttca aaatcaaaat tactcgttcc
     1141 ttttactggc atcttaactt ttcctccttt gactcacttc aatagatata ccactcatgt
     1201 tcattccatt gtttactagt taattggagt tttatcttag ggctttgtgc actctttcca
     1261 aaaacttgag cgtcgttttt ttcttctaac caaaaaaggt aatttcttac attcggtact
     1321 aaatgttttt cctgcatttc ttcatgaaaa acatataatt ctgcttcatt tttcaaccaa
     1381 ttcattattc attccctcat tcacttcttc ataccgccat tcagatagtg agatttcttt
     1441 ttgtttctcc acttgtccta taaaaatcgc tgtattcaac aagctgatca cacttaataa
     1501 taaagcaatg aaactaatgg ttacacttac tctttcacat tttgtcattt tgtttccttc
     1561 tttactcgtt tgttatttct tgtaataggt ctaataactc cttcaccgaa atttgcaaat
     1621 ggttcgcaaa aatttcaata aaagcactcg cacacattac cagaccttca actgatatat
     1681 cactgcgaca ataaaattct ggagaaacac tgatttctga ttctttctta caattgaacg
     1741 aaaaaactat ttttgaatca aattccggta attcttccac aactatgttt tgggattcta
     1801 ccaagaaagg gagtgcctgt tctaatgctt cttctggtgt ttgagcagat agaataattg
     1861 cttcttgttc ttttatttca ttgttctcaa atactttata actttttttc attgttagtt
     1921 cctctttcat tcctctctca attagctaag ccgttactgg tacgccattc tctaaattta
     1981 gtatcgctaa tccttaatgc tcgtcgaata tcaatcattc gataacctat tgcacgaagt
     2041 tctttatatc ttttagcagt tagcgtctct aacgtcattt caggcaatgg gttcgaatca
     2101 aaaatagaat tcgaaccgat tatttgggcc aattgttgta cttgtaacag ctcttctgaa
     2161 cttaattcag tgattgctcg attgcctatc attgcataca ttttctgtcg aatatacttc
     2221 ttttcttgtt tagacaacat ttctatccac tcctattttc attatcattt gttacctcca
     2281 cttcagtaaa tccacaaata aatgaattca tattccaagt accattccct tcaactacaa
     2341 cttttctcct gtcctctttc ggatattcaa gaatcagacc gtttaccaat atgtttttca
     2401 ctactactaa tttgtttgta taccgtggaa ttgctgttcc gatatacttc actttatttc
     2461 ctacgctaat tgtcatttcc tcactcctat tcttttttat ccaaaacttt ttcctgtcct
     2521 attcctgcca acgaatatac ttcactgcat gcttaaacaa aacaaaatgt tgccctgttt
     2581 tatttccgtc ttttacttgt tccacataca gttcatattt ggtcacacct tttaaaacgc
     2641 cagatacttt ttttcatcca ttgtcacaat tagttttttc atcaatcgat tgatactgca
     2701 ctgctcattt ttcttgttca tatagtattc ctttcttttt caaaactagc ctcttttgtt
     2761 tactagtttt catcaatcat caacgattta acagattttt tctttcgcag ggtttctagc
     2821 gtcagcaacc ccaaactcta aaaaagtgcc tttgctccct tgaaaaacga gcggacgatt
     2881 gtactcgtca tcatatgttt ttcatactac ctgatccacg caaataacct ttttttgttc
     2941 tgtgccacac ccatccatac gtatcgcttg agattcacca tactttttta ggtggtttat
     3001 ccttcctaag tccctgttag acacgctgat taacttagaa agtcctgttt gtatccaatt
     3061 ttcaaagatc tcccaaaaga aataagtagg tttacgtgta tcgcttctgt tcaaagtacc
     3121 tgtttgtact tgagtatttc tactcccctc gttcaaggga ttccttccac cagctagctt
     3181 ttcctttcat catcaaatgt tcctgaaaag catttacgtt tcgtgtccgt gtgcttactt
     3241 attctttttt cgatagcgat tatccgctcg ctactcgcta ttttatactt tgtttgttag
     3301 ctcaaataaa aactaacaaa caaaaaaggc tagtttcttt ccgttttatt tttttaactg
     3361 tcgataaata gttgttagtt taattccaag ttctaaaacc aatacgccca acaaaaccac
     3421 aggaaaaaca attggggcaa acaagtaaaa tcggatagtt ttcatgtctt ccctctttct
     3481 ttttggtgtc gctgtggtcg ttcttgccgt tcaatacttc gatacatttt ttattcatta
     3541 tttgctcgtt ttgttttcct ctggactgct tttcttgctt tttgaatact ttatacgctt
     3601 ttcgtgcctg aattaatagg tcttgtcggc gtttcattcg tgagaaaaca atatttgaaa
     3661 ccatcaaatc ctggttcgaa caaccacgat tcgcaataat tttcccatca aaaaaaatag
     3721 ttttcacttg agaaaaaaac agttggaaat cttgttttgt tgctaatttt tttgtcaaaa
     3781 tcactgtgct tttccctgtt gaatctacaa tgattcttcg aacaatccga ttcgataact
     3841 tccaacgttg ccgattaaac aatgttactt cacaacaaat atgacgtaag tgattagggg
     3901 tcattttcat gtgtccaatt cctccaacca attatgcatc gaaattttgg gcaattgtga
     3961 cagcccataa atctgcgttt ccaggtcttc ataactacaa gaacgcttgg cttgaagtaa
     4021 tgaatattct gcttgatctg atagttttgt tgccattgga tctagattca attctcttac
     4081 acaacattcc caaaaattca atagcgctct aaaataaaat ccttttcgat tctgaattgc
     4141 ttttccttca tgacgagcaa ttttttcatt ttgaatacac cgttgatacg ttttgcttaa
     4201 atcttctgca aatgtttccc ctaaaattcg atactcgctt ccatcagata aaccatgtgt
     4261 ttgattatac tttcgttgcc gcttttctat aattcgttta ctgtcgtata tcatgtttag
     4321 ctgctctttc gcttcacgcc attcagctga actgcgaaca atccatgtca aaacatcttt
     4381 cggaacaaac gtactttcgc tgtaatgagc aataaactct ttttgccaat catcagccat
     4441 ttcaacagga ctagtcgtat cgatttttgt atctagattt tctgtatcta gattgttaga
     4501 agaattatta aataattgtg cagaattttc acatggattt ttttcgtcaa aatcatcgaa
     4561 aatcgctaaa ttatcaacag attcctcctc ttcttttcct tgtgcagaat ctacactggt
     4621 tgagtcaaca ggacctaatg aatacacatc attttctgtc gtatctaatt ctcctagata
     4681 caaccgagct ttgtttcttt ttcctgtttg ttgatcaaat cccatatcct ctatttctag
     4741 tagctcatat tctttcaatt cttttttgat tgtattaaca gaacgtctgc aacaattcaa
     4801 tgcattggct aattcttgtt ccgtaaattc aaaataaaca aaaccttgac tatctacttg
     4861 atttcgttga atggctaaat ctaagcgtga ccgaaaaacc atatacgcga tttttgcttt
     4921 atcagacaat gagcgatatt tttcacttgt aataaatacc tttggaaatt gatagtataa
     4981 ttcattgtat ttacgagttg cggtaaatct ttttgttgcc atcgttatta cacctcatct
     5041 tgtccatata ttaagaaaga attttggtgt tggtagacac gaacatcttc cattgtagca
     5101 atcaattctt ttagataaat tcgaccgaat tcaattgtta gtaagtcgta atcttctaat
     5161 tcatttttga tttcttcaat ttcttcttca gacacattga gtaaaatggc taattcttga
     5221 ttggtataag aaatatagac atgtttcttt tcatcaatcc gtttttcaat taaggattct
     5281 tctaagtact cattcagtaa gctataggcg tattttgctt gtgcagtcat cgcataatat
     5341 tttgacgatt ttataaagaa tagttttggc atatgaaaag ttgcttgtgt cagcacatct
     5401 gttgcgcgaa atttgttttt taccatattt tttcctccta gaaaaacacg caaaaaaagc
     5461 tctgtataaa tcgaattaca cagagtttct tcttgttgat atttaatttc taaaatacgt
     5521 ttcgggtaaa aactaaacac ttactaatat ttagattgta gaatcttcgc cttcatttat
     5581 tcattttaaa gagaaaaaga tcaagtgaaa tgtgcccaaa agtttgattc tattaatttt
     5641 taaacaccat tcataatata tttcctataa tgtaaaaagc gtcgatactt attaaagcat
     5701 cgacgttatt tttatagtaa atttatcaaa atttttgcca acattttttc cgattttttt
     5761 ccctatttct atctttcctt atctttc
//
LOCUS       DAZUKZ010000135         5755 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000135 DAZUKZ010000000
VERSION     DAZUKZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.140"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..1194)
                     /locus_tag="ACG8EF_002549"
     CDS             complement(64..1194)
                     /locus_tag="ACG8EF_002549"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFS4947811.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1253..1480)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1842..3044)
                     /locus_tag="ACG8EF_002550"
     CDS             complement(1842..3044)
                     /locus_tag="ACG8EF_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4947812.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3104..3307)
                     /locus_tag="ACG8EF_002551"
     CDS             complement(3104..3307)
                     /locus_tag="ACG8EF_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS4947813.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3449..4516)
                     /locus_tag="ACG8EF_002552"
     CDS             complement(3449..4516)
                     /locus_tag="ACG8EF_002552"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4947814.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4574..4681)
                     /locus_tag="ACG8EF_002553"
     CDS             complement(4574..4681)
                     /locus_tag="ACG8EF_002553"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4947815.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4844..5656
                     /locus_tag="ACG8EF_002554"
     CDS             4844..5656
                     /locus_tag="ACG8EF_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4947816.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1694 a         1189 c          916 g         1956 t
ORIGIN      
        1 gatttctcca ctccatgaaa agccgacttt ttgggacgct tttttttgcc aagcctcaca
       61 tttttatagc caaacttctt ttgcgacttg ctgcaataat tggattttct tccattgttc
      121 ttcttctgtt aaatggtttc cttcttctgt tgaagcaaac ccacattgag gactgatact
      181 tagattttcc aatggtacaa attgttgtgc ttcttttatt cgttctttta ttttctccgt
      241 atcttccaaa tctggaaact tagaagtaac taatcctaaa acaactttac tttctttgtt
      301 actccaatgt cttaaaggag taaaatctcc tgctcggtca gaatcgtatt ccaaaaagaa
      361 accatcataa tttgttgaaa acaactcttc tgcaatgggt tcatatccac cggaaaacag
      421 ccaagaagaa gcgtgattcc cgcggcagat atgtgttgtg atcgtcaaat cttctggttt
      481 gtcttccagt gcgttattga taatctctgt acaaatatgg gccatttctt tcggatcaat
      541 tcctttttga agaagtgctt ctcttttagt ttgatcagct agaaacgccc aatttgtatc
      601 atcgaattgc aggtaacggc aacctttttc ataaaaagca tgaatcgctg ctttgtaagt
      661 agaggacaag tcctcataaa aatcagatag atcaggataa atctccttga tttttgaaga
      721 accatcatta gataggatct cttgtcgtaa aatcatcgtg ggactaggga tcgttgcttt
      781 tgcaacaaaa gtatcatctg tttccactac ttctttcaga aaagcaaaat cttttaaaaa
      841 tgggtgattt ggattgaaag ataatttatc tactacccga atattataag ctggtgctgc
      901 ttttccgtgg aatgtttgat tatatccagt ttctggaaca tagccttcga taccatttag
      961 ttcttctaaa aaatcaagat gccaaaatcc acgccgaaat tcaccatctg taattccttt
     1021 gatgccgact gctttttggt cttccactaa tttttttatt tcctggtctt cgattgcacg
     1081 aagttcttct tttgaaagta agccttcttg aacttttttt cttgcttctt tgatcgattg
     1141 cgggcgtaag aaacttccga catgatctac tgtaaatggt ctatttttgc tcatttcact
     1201 cactcttttc tttgtttttt gtaaaaaaaa tccttttact agcaaatact agtaaaagga
     1261 cgattatact cgtggtacca ccttttttca gaaaaaacat tctttctctc agcctgacaa
     1321 taaaatcagt ggtcttggta acgaaagact gttcgttttt gcctaccttt cgacaaaaaa
     1381 tcctctaagg ccattttcga cagtcaactt ctagcttttt acaccaacca aagcctctct
     1441 tgaaccattt tctgtctact ttccttctca tcggatacta ttagaataaa attagaatag
     1501 catgtttgaa agaaaagtca atagggaata agaacaaaca aaaactaatt tgttattctt
     1561 agtaatttgt ttgtggaata caataaagct tttctaatat gataattgtt cagtcgtcct
     1621 ttcatcttag atcacttcct taaaatgatc atacccgttt caaccacata aaaaaggact
     1681 taattttgaa aaatttccca tcaaaactaa gtccttttta ttatagttat gcaaatttgc
     1741 ttattttctc gctatttccg atacatcttg aacaggagca attcgactga aaacactggt
     1801 gcaaaagcat ttatttgtct tatggtagaa ccgtggaaaa ctcagactgg taataaattg
     1861 tcattttttg aaagaatagc gccctcttga acattatcaa tcgcacttac cacttgttga
     1921 tctgactttg catataagtg accgtaagtt cctaaagtga tttgaatatt cgcatggccc
     1981 aatcgttctt gaatagatag cggttgaata ttctgctcaa ttagaaatgc aacatgagaa
     2041 tgtcgtagcg cgtgaagctt aatcggtttg acacccgctt ttttagcagc ttcttttaac
     2101 cagttcgcaa aagaataagg tggcgtaaat gttccgtcca actgagcaac atattcaaaa
     2161 tttccaatac gtttttgcaa gtcataccac tgtctgagtt ttaaggaaag cttcttcccc
     2221 aacccaatcg ttcgataagc acatttagtt ttgggagtcg aaaagtacca atcatctttt
     2281 gtattataaa tcaatgtctt ctcaatcgtt atcgtgtttt tctcaaaatc tacattcgac
     2341 caagacaaag cttcaagttc tccaatccga atgccagtat aaaagagcat ttcatacaca
     2401 atcttccgat ggtgggtttg aatcttatca tccttcaatt cattcatcac tttttgaaac
     2461 tcactgtgtt cccaaaactc aactttctta ttctcagaag gaagcgaaga aacatcttca
     2521 acgggatttt tagcaatcac cccctcttta acagcccgat ccaatacaat ttttaggtgc
     2581 cctcgaatcc gattgatata agattttgat aacgtttcgc cattaacttt tttccgtgaa
     2641 ttacgtgact ctgtctggct tagttcaaat aaccaatcct caatatcgtg acttgtaata
     2701 tctcgaacac gaaaatgata gaaaaactgc atacgctttt cgaaaatgaa ttgcgcattc
     2761 tcatatgttt gacttttgac ctgtctctcg taccatttta caaacctatc cttgtaaaac
     2821 tcttcgaaag tcatttgatt ggtagaacac agttgattag gaattgtctt gttaaaatca
     2881 tgtgtttcca tgtacttgtc ataagcaatt ttggcatctt tttgtgaagc ataacctcgt
     2941 ttagtcttct gaacctttct tccattttca tctataccta gatacgctcg gaagaaccat
     3001 ttttttgtct ttttatcttg gtaaatattt ttaatttttg gcatcgttcc attcctccct
     3061 gtacttgaga aaagctactt taatagtacc gcgcttgtac tgtttattcc gcaacttgaa
     3121 tgtgaaaaag atgttcaatt actcgggatg gcaccacacc aacttggcga ttattataaa
     3181 aatcaatacc ttcaatcctt gcaagatatg ccttgctctc tttaatcatt cgtgcagctt
     3241 gactaggttt aaagcccatc tctatcaaaa ttgcacgatt taccatgaaa tcacattttg
     3301 tttccattct ctcacctcct gattctttac tccttttaaa ataatcctct ttataatcta
     3361 ttgtttctca ttcgatggtt gtgttatact cgtcttgtcg aggcggagta cgttgcacaa
     3421 cttgttgtgt aagcagtact ttgctttttt agtagcttgt tttctcattt gatatacttt
     3481 gtaattttcg ttcataatac tcaacctcct cctgataata ctccgggtct gccttcatat
     3541 tttggagaag tttgatctgt cttggattca tttctccttg ttcaataatc aaaggcaaca
     3601 aggaagaatg aaacgtttga tcgacctgtg caataaaaac taatgatggt gaaacaccat
     3661 gaattaacca actcatagtt ttctcaaaac tttgaggaat tgtatctaaa gaaatggtga
     3721 ttgcgccata attgctaata aaggctgccc atttggcatc aacttttgca cctagatcat
     3781 ttggatcacg gtcataaaaa caaattgctc cattaagtaa ctcaaagaac agtttggtta
     3841 aatcatgggt ttttgaaata atctttgcca agctttgcgc tttctctttt cgataccgta
     3901 gttcaaagcg attctttact tttgcctctt ctaaaggaat tcctcgtcgc ttccgttgtt
     3961 catggtcctt ctgataaaag caaaaacgac actgactctt ctttgagcct aaatacaagg
     4021 tctcaccatt tgattcattg tttatgatat ccgtaccgcc atgagattca ctcacttgaa
     4081 aagtggtcca cacatggcct agtattacct tatgcttaag ttctggaatg ctgagactcc
     4141 ccacaaaatc atctaaagta aaatcaatcc gtgtaaagtt cccctgataa tctaatacct
     4201 tacgaaaaaa ctgttgccaa tttgtttttc gtgtttttag ccatcttgct aagtgtttac
     4261 agccttgacc tgaaaactcc atcacagttc ctttaaccgc atcgtcaatg gaataccgaa
     4321 tcgtgattac ttgattccac acgtattggc caatatagcc ctttggcgca taatccagtt
     4381 cctcaaaacg ttcaatgggc atttcaataa tctcttcaat tagctttaaa tgttgattcg
     4441 agaaaaagtg aatggttaga taatcaatca aaacacggtt aactggaacg acctcttctt
     4501 gcaaactatc caacacaaga ttcaattttt cttcacttaa ttgtctcgtt cctttttcta
     4561 ctttcgataa taactatctc gaaatcccca ctttttctgc taaatatcgt tgtgtccacc
     4621 ccctttcttc tcgagaaact cgaatggctt caccgagatc atgttgatta acttggttca
     4681 tcgacaaacc tccttctgct atttacttgt caaagaacct attgaactta ttttaagtac
     4741 atttttattg tacgcctgta tttattttaa gtcaatatgt tttgactttt tttaagtaca
     4801 cacgtatact aaatttaaga acttatcctg aggaggagtt ttcatggaga tagacaagca
     4861 agcagtcggc aatcgaattc ggcagattcg ccaagaatta aagttgagta tggagaaatt
     4921 tggcaaacta attggtgatt taccgcgaag tacagtcaat aactgggaac gtgggatcaa
     4981 ccttccaaaa accgaaaccc ttcaccaaat cgcagagata ggtcatgtca ccaatgaata
     5041 tcttttgtat ggtgatcaag agaatcaata cattttagaa atgctccaaa aaaaggcagg
     5101 taagcttgat cctaagattg aaggtctcat agtagacgaa atcaaacacg ctgaccttgt
     5161 tagtgaaaaa gaaatggatc gaatgattga attttttatt acaaatctca ttccaccaac
     5221 tgaacaagac cagtttactt tccaatgtat cgatgaaaaa aagcagcttt atcttggttc
     5281 aactaatttt ggtaaggaag cccagctcta tttgcaacat gataatcaaa atcatattct
     5341 gcacatgatg ccctttacct tctccaactt tagtgtggat cgcatactcg tctatttagc
     5401 aaatcaaaaa tcctattcct attttgggaa acatttaccc aaggaactag gtgagaaagc
     5461 catccttcta tactcaatta atcaattgac cagcgatgtg cgaattgccc cgttggtcta
     5521 ttcagaagaa accgcttctt atcagtacac agaggacaat caatatctac tagaacagca
     5581 atatctttat ctaccattcg tgatggaagt cgagaaagaa cgcctgttaa atgcagcgta
     5641 tccatcatca aaatgaggtg acaaaattga taagcacgtg acaaagaaaa tactgctagg
     5701 acactcccca atgggtagtt gtcacagttt cctgttcttt gttagccccc tgtta
//
LOCUS       DAZUKZ010000136         5684 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000136 DAZUKZ010000000
VERSION     DAZUKZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.141"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      138..304
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            452..1009
                     /locus_tag="ACG8EF_002555"
                     /pseudo
     CDS             452..1009
                     /locus_tag="ACG8EF_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1067..3106
                     /locus_tag="ACG8EF_002556"
     CDS             1067..3106
                     /locus_tag="ACG8EF_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS4947817.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3215..4366)
                     /locus_tag="ACG8EF_002557"
     CDS             complement(3215..4366)
                     /locus_tag="ACG8EF_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS4947818.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4520..5050)
                     /locus_tag="ACG8EF_002558"
     CDS             complement(4520..5050)
                     /locus_tag="ACG8EF_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS4947819.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5149..5499)
                     /locus_tag="ACG8EF_002559"
     CDS             complement(5149..5499)
                     /locus_tag="ACG8EF_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFS4947820.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         2048 a         1040 c         1061 g         1535 t
ORIGIN      
        1 tgcaacggga ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta
       61 aatttcacta tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac
      121 tcgtaaatag aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt
      181 tgcccagaaa cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt
      241 tccaatagct aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg
      301 atgctaggtc agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac
      361 tgacttattt gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag
      421 gagatgcggt gaaaaaagat cgattggtgg aggtcgctcg gattcacttc tggttgcaca
      481 atacaaccag aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga
      541 tattccaggt tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt
      601 attaaatcaa acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac
      661 atttcaagat tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc
      721 agaaaaatct ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg
      781 taatacttta ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat
      841 cagaaggcag cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat
      901 attcaagcta cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga
      961 aagtgtgctt ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt
     1021 ataaatagtg aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga
     1081 caagcacggc aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc
     1141 tgcgggtggc ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg
     1201 agaaaagaca gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc
     1261 agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga
     1321 taaataccag aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa
     1381 agtagataaa aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc
     1441 attaggtgaa ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac
     1501 cacgatcaac tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag
     1561 cctgaccaca caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc
     1621 aacaacaggc aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga
     1681 aaaaacagcc aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa
     1741 tcaggcgatt tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga
     1801 tggagcaacg ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta
     1861 tcctttgggt gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga
     1921 tattgataag aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat
     1981 ggcttttgat aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga
     2041 cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt
     2101 gacaatcgat gcgaaggcac aaaaaatagc ttttgacagt ctaggaggaa aagctgcatc
     2161 aactgttgcg acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta
     2221 tgatccaaac aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa
     2281 tcctgaacat ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa
     2341 aatgattaca gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac
     2401 gatcaacggg cttaaatggc aaaaagacag ttcttgggga tcttttaaaa caacgcgtgt
     2461 cagtagtgat gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata
     2521 tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt
     2581 catttttggt gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga
     2641 agatagcttt aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct
     2701 aattactcct atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt
     2761 ctatcctaaa ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac
     2821 agcagtacaa acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc
     2881 acattctctt tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa
     2941 agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa
     3001 ccaaggttat atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa
     3061 acgagcatcg gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta
     3121 agtacagtaa aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc
     3181 acgatgtctc tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa
     3241 aatatttgag aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg
     3301 aatagtactg tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca
     3361 tttctccacg cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg
     3421 caatctgagc accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata
     3481 tagaaataaa gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga
     3541 caaaaaatac tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt
     3601 ctttaacctt tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa
     3661 gcccaacaag atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa
     3721 tagaagaatt tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga
     3781 ctaataagaa aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag
     3841 cattgctaga agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta
     3901 aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt
     3961 ttagttcttt taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa
     4021 agaaaatcgc ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa
     4081 ccgggaagat gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa
     4141 gagataaatc actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa
     4201 agtcatggac tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac
     4261 caacaagtaa ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa
     4321 tcgttgttga aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt
     4381 tgtataaaat atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata
     4441 ctcacaaaaa aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta
     4501 ttttaataac taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa
     4561 tctcctggat gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac
     4621 ttttgaatgg attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa
     4681 cactccaact tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga
     4741 taacagctaa tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga
     4801 cgatcagagc taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg
     4861 ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg
     4921 cggtgatcag ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc
     4981 cagaaataat ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg
     5041 tctcaaacat ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca
     5101 aattaaatgt tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag
     5161 gatcttttcc atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat
     5221 ttctttcatc aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat
     5281 caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg
     5341 tttttgtagt tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc
     5401 atctacttcc tcttttatac gatcttttct ctccgctttt gctacataca taggataaac
     5461 gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt
     5521 tcaaattatt tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa
     5581 aaacaaaggt tctataatat tttgtgttgg tggaaaatcg aaggcgattt tctactaaca
     5641 ctttttcttc gtttcaactc aaaaagagac tcacttttgg taaa
//
LOCUS       DAZUKZ010000137         5623 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000137 DAZUKZ010000000
VERSION     DAZUKZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.142"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..106
                     /locus_tag="ACG8EF_002560"
     CDS             <1..106
                     /locus_tag="ACG8EF_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4947821.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            143..2008
                     /locus_tag="ACG8EF_002561"
     CDS             143..2008
                     /locus_tag="ACG8EF_002561"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4947822.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            2022..3465
                     /locus_tag="ACG8EF_002562"
                     /pseudo
     CDS             2022..3465
                     /locus_tag="ACG8EF_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            3573..4274
                     /locus_tag="ACG8EF_002563"
     CDS             3573..4274
                     /locus_tag="ACG8EF_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4947823.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            4400..4912
                     /locus_tag="ACG8EF_002564"
                     /pseudo
     CDS             4400..4912
                     /locus_tag="ACG8EF_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(5310..5519)
                     /locus_tag="ACG8EF_002565"
     CDS             complement(5310..5519)
                     /locus_tag="ACG8EF_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFS4947824.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
BASE COUNT         1799 a          952 c         1260 g         1612 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagaaaata
      121 aaaaagaaag aaggaaaaaa caatggataa tcaagcagtt ggtcgtcgtg tatgggaagc
      181 ggtaggagga caaaagaatg tcaaaagttt agtacattgt gctacgcggt tgcgattcag
      241 attaaaagat gaatctttag cagatacgca aaagttaaaa gaagacccgg acgtgatcca
      301 agtcgtacaa agtggcggtc agtatcaagt agtgattggc agtaatgtag cagatgttta
      361 tcaatcgatc gtggatgagg aaggtcttgc agtttcggag gatggaaaag aagagtccaa
      421 aaatattttg aatcgtctga tcgatattat ctctagtata tttacacctt ttctaggggc
      481 gatggcagcc gccggggtat tgaaaggatt cttatcattg gcaacggtga tgggctggct
      541 gacagctgat agcggtactt atcaaatatt gtttgctgca gcagatggtg tctttacctt
      601 cttacctgtc atgcttgctt ttacagcagc taaaaagttc aaagccaacc agtttttagc
      661 agtagcaatt gctatggcac ttgtgtatcc agctatcaca gcagttgctg gtgcgggaga
      721 agctatcagt ttctttggga tcccagtgat tctttcacca tctggttata cttcttctgt
      781 cataccaatc attctagccg tatgggttca aagtaaatta gagccattcg taaaaaaagt
      841 gattccgcaa tttctgcaaa tgattttagt tccgcttgtc gttttagtag tgatggttcc
      901 attaacattt cttgctttag gaccaatcgg gacggttgcc ggtaatgctt taggcggtct
      961 cttcaactcg atttatggat ttagtccaat cgtagcaggt ctaatcatgg gaagtttatg
     1021 gcaagtattc gtgatgttcg gcatgcattg gggattcgta ccgatcatgt ttttgaatat
     1081 cgaacaatat gggtttgatg tattgatgcc aatgcttctt ccagccatct tggcacaagg
     1141 tggggctgct ttagctgtag cattgcgaac aaaagatacg aaacttcgag cattagggat
     1201 ttcttctaca gtgacttcgt tatttggaat cactgaacca actgtgtatg gtgtgacatt
     1261 acctttgaag aaaccattca ttgccgcttg tatttctgga ggtattggtg gagctattat
     1321 cggattttcc ggagtaaaag ctttctccag cagtttggtc agcttattga cgattccgac
     1381 tttcattaat acggtggatg gtgtagagtc aaatgtgaca gtagcagtaa tagctacagg
     1441 aattgctttt gttcttgcat tcgtaggtac attgatttta ggatttgacg aacaaacaca
     1501 agacaatcaa ttagaaaaca aacatgcgaa tgcaggaaaa ccgattactt cagcacggca
     1561 taccttaaaa agtcctttaa cgggtaaagt actgccattg agtgaagtac cagaccaagt
     1621 tttttcttct ggcgtgatgg gaaaaggtat cgctattgat ccagaagtag gagaactagt
     1681 tgcacctgca gacggagaga tcacgactat ttttccaact ggacatgctg tagggatcac
     1741 aacgactgac ggagcggaga ttttgattca tatcgggatg gatacagtag aattaaatgg
     1801 gaatggcttt gagatcttag tcaaacaagg ggatctcgtc aaagcaggag atttattgat
     1861 tcgttttgac atcgaagcaa ttagagcagc tggctatagc gtaatcactc cagttgtcat
     1921 tacgaatact gatgcatttg ccgatatact tgaattagac caaaaagaga ttattgcaaa
     1981 cgaagatgtt ctggcaattg taaaataagg aggaaatcag gatgcaatca cgtttttcag
     2041 aaaaattttt atggggtgga gcaactgctg ccaaatcaat tagaaggggc ttatgatcga
     2101 gacggaaaag gactatccgt tgcagatgct atgcctggag gtaagcagcg ctttgcgatt
     2161 atcggcagtg aagaatttga ctggacgatt gatcaagaaa aatatattta cccgaatcat
     2221 cgaggaatcg atcattacga tcgtttcaaa gaagatcttg ccttgtttgc ggaaatggga
     2281 ttcaaatgtt atcgtttttc gattgcctgg acacgaattt ttccaaatgg agacgaaggt
     2341 actcctaacg aagcgggatt agaattttat gatcagctga ttgatgagtg cttaaaatat
     2401 gatattgaac ctgtgatcac gatttctcat tacgaaatgc ctcttcattt ggcaaaagaa
     2461 tacggtggct ggaaaaatcg aaaactgatc gacttctatg aacgatttgc tcagacagta
     2521 ttagagagat acagcagtaa agtcaaatat tggatgactt tcaatgaaat caattcggcc
     2581 tttcactttc cagctttaag tcaaggatta gtaaaaagca atggagcagg agagtaccaa
     2641 aatattttcc aagcatggca taatcaattc gttgcaagta gcaaagccgt aaaaatcggc
     2701 cacgaattgc gttcggatat ccaaatcggc tgcatgatta tctatgcaac aacttacagc
     2761 atcgatgcca atccagtcaa tcaggcagca acaatgatcc aaaaccaaga attcaatttt
     2821 ttctgcacgg atgtccaagt acgtggagaa taccctgcat acacagcgcg tacccataaa
     2881 aaatatggag tcgatccgga aaaattagag caaacggaaa aagacttcca gttgctaaaa
     2941 gaatatccag tggactacat cggatttagt tattatatgt ctacggcaat caatgagact
     3001 gatccacaag cagcaacttc agaagggaac ttgctgggag gagtaaaaaa tccgtttctt
     3061 gaggcaagtg aatggggctg gcagatcgat ccagaagggc tgagaatcgc attgaatgaa
     3121 ttatacaatc gttaccagaa accattattc attgttgaaa acggtcttgg agccattgat
     3181 catatggaag aggatggcac gattattgat gattatcgga tcgattattt acgtcgtcat
     3241 atcgaagcga tggctaatgc agtagcagac ggtgtagatt tgatgggcta tacgccttgg
     3301 ggatgtatcg atcttgtcag tgcatcaaca ggagaaatga gtaaacgtta cggctttatc
     3361 tatgtggatc tagacgatga aggaaatggg acactgaatc gatcaaagaa aaaatctttt
     3421 gattggtata aagaagtaat tgcctcaaat ggcgtaaact tgtaattaaa gcagctgaat
     3481 gaaaaagaac acacttctca catgtgattt gtgttctttt tttatttttt atgtgctatt
     3541 cttttagtaa tagtaaagca aagggtgacg ttatgaaaag gacacgtgta ctatacttgg
     3601 aagtcgcaga taaaattaaa gaagatattt tctcaggaaa atatccagtc ggttcgatgt
     3661 tgccgacaga aagtgaatta gaagaactgt ttcaagtcag caaaatcact gtccgaaagg
     3721 caatcgaact gctggcatca gatgaatatg tcgcaaagaa aagtggtcgt gggacgacgg
     3781 ttctaagcaa tcgtccttac aatcgattat ccaaagcggc aagctttacg caaatcttgg
     3841 agaactcccg atatgaagtc cggaaagaaa cgctggattt gcaattggtc catataaaga
     3901 aggaagatcc tttgttttcc tttttcggtg agcaagctgt ttgttttaag cgcttatatt
     3961 atttgaacaa tcagccttat atttattttg ttcattatct tccacaagaa atgatgcaat
     4021 tcagcagaga agaatttggc aaagaatctt tgtatcgttt gctgacgaaa aaaggatata
     4081 ctattgactc attttctgat gatttctcgg cagtattttt aacagaagaa gaacaaggaa
     4141 ttctgaaaac agacgcacag atcggcatca aacggatacg gaaatcgtct acgcaaattg
     4201 gggaaatgat tgaatattcg gaggcggtct accatacgga gctgcatcct taccatattg
     4261 attatgaaac ataaataaaa aaagaggttg tgaaaaagct gccctgcatc aagtaataag
     4321 aacagccaaa caaaaatagc ttctcatatt tttcgtttgg ttaggcttat taccgcagat
     4381 acacggctgt ttctggatta ccatttattc tggataaggg ggtgtggcac gattttcaga
     4441 aggaatctat gaaccaatca ctgtattgat tttagcttcg atggtaaccc cattcggctt
     4501 gacgatcgct tatttcttag ggaaaatcat tcgtaaaaat atactcaatc gtcaagaaat
     4561 cgatacattg aagaccgctt ttcctatggg gatctgtcag atcacagagg gatgtttccc
     4621 tatcgtattg aacgacttgg cacgcaatgt catagcaacg ggagtcggtg ggacagtagg
     4681 tggcggatta agcatgtttt ggggcgcaga tagccgtatt ccggcatcgg gaatgtttgc
     4741 agtcgctacg atgaccagac catgggcatt cattggtgca ttgttagctg gttcttttgt
     4801 cacaggaata accattttat tattgaaaaa accagtagat ccgaatgcag aaatcatcca
     4861 agaagaaaaa gaggaagaag acatatcttg ggatgatttg acaattagtt aggagaggca
     4921 gaatggatta caagaaaaaa gtacaataga tgtaccttga gacaggaatg gtatgaaaga
     4981 atcagagtta gaaagaatcg aatatacaga ttttggttta ggagcgatcg aaactgtcca
     5041 gagcatcggc accctccttg aaatgacgat gaaggaaaaa tgagaacctt tcgttgtcga
     5101 aagggtctgg tataccttat gtcgaaggaa aggaaacaaa aaaactttcc tgtactattc
     5161 caaaaaggaa agaaagttat tatacagtaa aaaaagaaat tattcttcat gctagagatc
     5221 agtacacgtt cgagcccaat atcaaacact agttccaggc aggcgcaaca ggtgcggtta
     5281 tcactgaatt ttcttccagc agtgacgatt caagtgatat ttttacaaat ccagctattt
     5341 atcgttaaca aaaagatttt taggagaagc tgatgaacaa aaatgatcaa attttgatcg
     5401 tttagttcgt cggctttttt atttagagaa acatcattta tcagtgaaac ataacacgag
     5461 aacaaaatga ttttttcgtt aataaaagaa acaagtattt gttgtttaaa ataatacacg
     5521 ttattggcat ataggacgaa atcccagtat cttctataag taagtcattt catatcgatg
     5581 aaaacacgtt atttattttt gttttatcta taaaatggct gaa
//
LOCUS       DAZUKZ010000138         5615 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000138 DAZUKZ010000000
VERSION     DAZUKZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.143"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..2009)
                     /locus_tag="ACG8EF_002566"
     CDS             complement(60..2009)
                     /locus_tag="ACG8EF_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4947825.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2051..3109)
                     /locus_tag="ACG8EF_002567"
     CDS             complement(2051..3109)
                     /locus_tag="ACG8EF_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFS4947826.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3228..3683)
                     /locus_tag="ACG8EF_002568"
     CDS             complement(3228..3683)
                     /locus_tag="ACG8EF_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947827.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4267..5070)
                     /locus_tag="ACG8EF_002569"
     CDS             complement(4267..5070)
                     /locus_tag="ACG8EF_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4947828.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2077 t
ORIGIN      
        1 tcttacgacc catcgtttta ttctaaggtt ttgtagtaaa tatttcttga atttcatagc
       61 tacgcccctc caatcgaaaa tacaatcttg ttttctttac tttcagcaac ggaaggcttt
      121 gcaataattt cagcattaga atcacctttt ccttcaaacg ctccggcttc actcaactta
      181 acatcgaata tttttgcgtt tggataagta attattgctt cagtaatcgc ttcttcaaac
      241 tcagattcaa ctaaatgcaa gatattcctt gcaccccctg atttagaatc ctcttgtgaa
      301 gccatgtcaa aaactaagtg catggagaga tcgtctgcat aaaaattttg accatatact
      361 ctttctttcc taggtggaag acgtaaatct atatttaaat gttttcgtac taattcagca
      421 ctcttcaaca tttcattata agcaatttcc agaagagttt gttcggataa agcccaaaat
      481 ggaactattc gtccaaatcg gttaataact tcaggcttta gaccttcacc aatcaaggct
      541 tgtttgatcc ctgatgcgac ttttcggtat tctcttttta gttcagtgct gtattcatta
      601 gaattttcct tgtttgtaac acgagatagt gagctatatc ggtttaacga atcgaacatc
      661 tccgcacctg catttgttgt cgcaaataaa attgtatttg acaatgaaac ggtgtcgtga
      721 acttctcgat catcccatcc tctttcagta aaacttatcc taccttcatc taaaattgat
      781 aaaaaggcat cgagtgtttc tacaccagct ttctcatatt catcacacac aataatcgca
      841 gaaggatcat gccttacatg agtacctata agtcgtctaa aacgcattgc atcatctttg
      901 gttttgtagt ccggcatatt aactaagaga actttatttt catcgccata aagtcctctt
      961 gccacggctt tagccattgc agttttaccg acccctgttg atcctgtaaa cagagcagtt
     1021 gcttttggtt gctttcgacc tttaaaacga tcaaatccaa gcttcatacg tttaaacaac
     1081 attgttatag tataaactgc taacggttgt ccttttatgg actttaattc ctcttgaagt
     1141 ttttccatat caaaatcaga atctagctga atattatact taccaaatat ttgcaataga
     1201 ctttctcgat tataagcatt tccgtctact cgatcttttg caatcaaatc ctccattaca
     1261 gcaattgatt tagccggttc ggacaaatcc tcacgataaa gtttgtttgc ttgtacgatt
     1321 tcttgaagta attcatcagg aatcctttgc gataatctaa aagaagcctg aggatcatct
     1381 ttaacagagg atgtatttag ccattgtctc aaaatatcga cgacaacatc gctttttagt
     1441 tcattcaatt ctaacggttg tagccgtctc cctaaggcat catctctttt gatatagttt
     1501 tcatattctt ttcttgtagt agcggtaatc atttgaagtg attcatttgc taacggttct
     1561 tttagcgaat cacccccaat tttattgcca ctcccaaaag agctaacaac tttatgaaac
     1621 tcgtcaatga agaggacaaa ttcattatct ttgtcctcag cttttaaaat tcgctcatat
     1681 tctttcaatc ttggaattaa tgtttctaaa cgtgtttgga ggacatcatc tcccccaaca
     1741 cgcaattgtc cgattgataa tttatatact tgtactttct ttccatcttg tcgctgtctt
     1801 tcagcccatt cacttgcaag tgctgtttta cctgttccgg cttcacctaa caagatagct
     1861 tgcggtgttt ccggtctatt caagatcagt gacaactcat ggagttcgtc atcacgtccc
     1921 actaacttat gttttggtgg tttcattggt gtgagagcat gattaaattc ggtttgaata
     1981 ttcttaaaaa actcggtact attttccatc tgtttgtgtt cacttcctct aaaataagaa
     2041 taatttactt ctagtcagtc aaagttaggt acttaaatgt cgtcttcatg tctccaaaag
     2101 aatctttgtt aaagtcagca tttttgtatt gatcaatcaa tttttgtgta tcattgattg
     2161 ctttaatttc ggtctccatt cgactgtcta gttgacgaat actttcttgc gaatctttat
     2221 cgtcaggatt ttgttgtaaa cgttccagat actcagcttt tgtggcttca attttcttga
     2281 tgttagtatt tgaagcattt aactgatctt ctagcttatt tacaacatta ttcttacttg
     2341 taactgctac aacttttgaa taatcaatat cgccatcttt ggtaagaaag cttccttgat
     2401 agacattagt tgaatctgaa tatggattta ttctgaaatt gatattatca ggagtattaa
     2461 caggttttgc ttttttccca aaagaaatta ctccttcatt attaacatca ttcatagaat
     2521 attgagaaag tgcttttgta actgaatctg cttcattcac attgactttg gtatcatcgt
     2581 cagctgtatc atcaccagaa attagtgata cttggtttgc aataaaaact tgataagctc
     2641 tttcctctaa tttacctttt aggaataaga aagcatcccc atcagtactt aacatcccat
     2701 agctcatttc aagcttctta ggacgatctt tttcttttgt tgccatatat aagttatagt
     2761 tttttccttg cgtagataac aactttctag cgttttggga gtaaccaagt tttactactg
     2821 ttacatctct attttgatct gtccacacat ctgaaatagt gatttctgct cctgatcgag
     2881 caaactccaa tgaagaccct ataggtgttg tattgttcac gagataataa ttatagtttc
     2941 ctaattgata aatgtagcta cccaagcctg ttaataaaaa aagcactcct agaaaagttg
     3001 caagcataat gcgtaatccg tgacgtgttc tcttatcctt atcaccaagc aaccctccaa
     3061 taatactgta ttgatctttg gtagatgttg aattgacgtt tttttccatc agtttgtctg
     3121 ccccctttca taaaagagca aacaatcaga cgttttttcg gtaattctag gttcgggatt
     3181 ttgaatcatt tcggtatatt cttccccatt aatatttaac acaaaaatta tcttcctttc
     3241 ttcgttcctt tcttttgctc tttattttcc aaataatcaa tgatttttgc tattgtacca
     3301 agatagtaat tcccctcagc tttgtttttt cggattttgc taaccattgg agtaggtaca
     3361 atctcttcat ctctcaattc actgaaagat tttttaccta gatattttcc aataacagat
     3421 tttaaaaaac gttcaaaatc tttctcgtaa gttgttttct tgccttttag ttcatggttc
     3481 atattgtcaa cgacttttgc ggtttcaata attgttttag ttctcagctt aataatttca
     3541 tctggaattt tcaatcctcg taatttcatc gttgtactaa ctggtaaccc atgctttact
     3601 gcatcagcaa tactaaaact ttttagatca tctagtaagc tctccatata gttaacaaat
     3661 tcatgccaat taatagcatt cattgtctca ccccttcatt tttttaaaat gaggttcggg
     3721 cgtaacacat ttatgttatt ataatatcat aataacataa ataaatcaat cagcaaattt
     3781 agatatccct taaagtatca gcaattattc aacaagatat ttattttagc tatttcagtc
     3841 atcataaaag actaattagc aataaaataa ctaatattat ccatttatca gacgaaaaga
     3901 aaaaacttat ctaattactc tatattaact tttaacaaat agttgacttg tccaaaaatg
     3961 ttaccttcaa tctcacaaag atattaagat gtttcctacc aaaactgata tcaccggact
     4021 ttttattctg tgttgtccaa aagtgttacc tttagtcgat caacaaatta aaaaaaattg
     4081 tttttttctt gacttgtcca aaagtgttac ccatagttgt taaaatttgt atagattagc
     4141 cattctttcg aatattattt agactgaaac ttgcttacaa aaaaacttct tgcatttatt
     4201 gtccaaaagt gttacccata cttttttaaa gaagcaggtt gtattaaaat tacaacctgc
     4261 ttcttctcaa tcttcttcta accaattatg cattgaaatg gttggcaagt ttgattctgg
     4321 ctcactaaaa gtgaatctga gccgatcaac tcttcttcct ttctttgcat agattttttc
     4381 ataatcaaaa gtttcaaata cctgtattcc gttttcatca ggagctaaaa attcttcttt
     4441 cgcctgctct agtattcttt tgtcaatatt tgtcattcta taacttgaag gtatacttaa
     4501 tgctcttcta aaatcttcaa ctttgactac ccaataacca tttcgccact ctttatttct
     4561 gtattgcatg agatgacgat acaattcttt tgtgtagcta cttcttagtt ttgtaaaatt
     4621 atcaagttca aaacgagtaa agtttttgcc aattgagttt aagataaatt caaattcctc
     4681 atttgcctta attgtaactg tttccttctg tttatctatc cggaattttt gaaaaagcca
     4741 cagctgttca tagacgtctg aatcttcaaa acggtaggtt aaagtaccta gtttgtcact
     4801 caaagctttt aacacagaat aaaaactgtc cttttctctt ctgtcgtatt tgaccaaata
     4861 acgaaagagg ttgaaatcaa aagttacttc tgctgttcct tgacgtttca tttttgataa
     4921 aatagcccaa aacacatcca tttcaacagg cttaaatcct cgcaaagctg ttgtattcag
     4981 accattgtta taacgcacaa ttgcatttgt ttgttctgat actaaatcta catcactatc
     5041 aataatcttt tcggtaactt cttgagacat tgattactct ccaatcttct ttttgcgctc
     5101 ctttaatcat gttaatctgg tactaattaa aggaaacaat tttttctgct tgttacctaa
     5161 attaaatcac aactaaaggt aacagtcaac aaagatttgt tactaatcta attttctatt
     5221 ttttcgcctt ccaaatcaag aaaaccgaag gtaacgtctt atcctagtgt gatacctcaa
     5281 atgtccactt ttgttacctt tgctgtccaa aagtgttacc cttactgtcc acttttgtta
     5341 cctttactgt ccaaaagtgt tacctttata cccctttaat acttactctc ccaagagatt
     5401 aagcctcccc taacagatat attaacagat atttaacaga tatattaaca gatatttaat
     5461 aaaggaggat tactcctctc ttatttcaaa atcttaaaaa taaaaaatct taaaaaaaac
     5521 taacactatc taggagttta ttttgcttgt gtaactttga ataattgaaa aaaaggaaaa
     5581 ggaggaagct tcagcttccc cttttattta tattt
//
LOCUS       DAZUKZ010000139         5534 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000139 DAZUKZ010000000
VERSION     DAZUKZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.145"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..988)
                     /locus_tag="ACG8EF_002570"
     CDS             complement(<1..988)
                     /locus_tag="ACG8EF_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFS4947829.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGN"
     gene            1140..1835
                     /gene="vanR-A"
                     /locus_tag="ACG8EF_002571"
     CDS             1140..1835
                     /gene="vanR-A"
                     /locus_tag="ACG8EF_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFS4947830.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1813..2967
                     /locus_tag="ACG8EF_002572"
     CDS             1813..2967
                     /locus_tag="ACG8EF_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFS4947831.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3182..4150
                     /locus_tag="ACG8EF_002573"
     CDS             3182..4150
                     /locus_tag="ACG8EF_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFS4947832.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4143..5174
                     /gene="vanA"
                     /locus_tag="ACG8EF_002574"
     CDS             4143..5174
                     /gene="vanA"
                     /locus_tag="ACG8EF_002574"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFS4947833.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5180..>5534
                     /locus_tag="ACG8EF_002575"
     CDS             5180..>5534
                     /locus_tag="ACG8EF_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFS4947834.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSR"
BASE COUNT         1670 a         1078 c         1251 g         1535 t
ORIGIN      
        1 tatttccgat aaccgtgtat tgaaaggcat cttcgaagcc attatccaat atttcgcagg
       61 cttttgtata tttaggctgg tcgatatatt caccgactaa ggcattctta gctgttcgag
      121 cgagttcaat atccgtaaac ttaaagatcg cttttactgc ttctctaaac ggttttgaat
      181 tcttttttgg aatggaactg aagatgtttc ttaaaaaatg gacctggcat ctctgccaac
      241 ttgcgttggt aaatgactta cgaatcgcag acactaggcc tttatgggca tcagaaatga
      301 tgagttctgt cccctttagg ccgcgttctt ttaagtattc aaagaagatg gaccatgtgt
      361 catcactttc ttcattttga atcatgaagc caatgatttc acggtcgcca ccttctgtta
      421 tcccgatcgc aatatggcag cttttagaaa gcactcgatg gtcctcacgg acttttatgt
      481 agagaacatc agtcatcaga taaggataat tcgtacctga gagtgaacgg ttctgccatt
      541 cgttgaccat cgggtctaac tgctcagtca ggctagaaac aaaagatttc gatacagatt
      601 ttccacatag ctcttcaaca atctttgaaa ctttacgtgt cgaaacgccc gaaacataca
      661 tctcaagcat tgaagcgagc agtgcctttt catttcgctg ataacgctca aacaccgtcg
      721 gtgaaaattc accatcacgt gttctaggca cttttaattc gagtgtaccc acacgagtcg
      781 taaagtctcg ctcataatag ccatttcttt gactctgacg actttctgaa cgttcatagt
      841 catcggcttg aatatattct gttcgttgat tttccatcaa ttggttgaaa acagtggtta
      901 aaatattttt agaaacatca tcttttaccg aatgttcaat aatactttga acctcttcgt
      961 tgttcagtgt aaaatgtact tgggtcatgt aacgtcctcc tgggtatgtt tttgtggtta
     1021 aaaacattgt accgtaaaag ggctgttaca tggcctttta tcttttacac aattatatgg
     1081 actttatcct ataatctgat aaattattaa gggcaaactt atgtgaaagg gtgataacta
     1141 tgagcgataa aatacttatt gtggatgatg aacatgaaat tgccgatttg gttgaattat
     1201 acttaaaaaa cgagaattat acggttttca aatactatac cgccaaagaa gcattggaat
     1261 gtatagacaa gtctgagatt gaccttgcca tattggacat catgcttccc ggcacaagcg
     1321 gccttactat ctgtcaaaaa ataagggaca agcacaccta tccgattatc atgctgaccg
     1381 ggaaagatac agaggtagat aaaattacag ggttaacaat cggcgcggat gattatataa
     1441 cgaagccctt tcgcccactg gagttaattg ctcgggtaaa ggcccagttg cgccgataca
     1501 aaaaattcag tggagtaaag gagcagaacg aaaatgttat cgtccactcc ggccttgtca
     1561 ttaatgttaa cacccatgag tgttatctga acgagaagca gttatccctt actcccaccg
     1621 agttttcaat actgcgaatc ctctgtgaaa acaaggggaa tgtggttagc tccgagctgc
     1681 tatttcatga gatatggggc gacgaatatt tcagcaagag caacaacacc atcaccgtgc
     1741 atatccggca tttgcgcgaa aaaatgaacg acaccattga taatccgaaa tatataaaaa
     1801 cggtatgggg ggttggttat aaaattgaaa aataaaaaaa acgactattc caaactagaa
     1861 cgaaaacttt acatgtatat cgttgcaatt gttgtggtag caattgtatt cgtgttgtat
     1921 attcgttcaa tgatccgagg gaaacttggg gattggatct taagtatttt ggaaaacaaa
     1981 tatgacttaa atcacctgga cgcgatgaaa ttatatcaat attccatacg gaacaatata
     2041 gatatcttta tttatgtggc gattgtcatt agtattctta ttctatgtcg cgtcatgctt
     2101 tcaaaattcg caaaatactt tgacgagata aataccggca ttgatgtact tattcagaac
     2161 gaagataaac aaattgagct ttctgcggaa atggatgtta tggaacaaaa gctcaacaca
     2221 ttaaaacgga ctctggaaaa gcgagagcag gatgcaaagc tggccgaaca aagaaaaaat
     2281 gacgttgtta tgtacttggc gcacgatatt aaaacgcccc ttacatccat tatcggttat
     2341 ttgagcctgc ttgacgaggc tccagacatg ccggtagatc aaaaggcaaa gtatgtgcat
     2401 atcacgttgg acaaagcgta tcgactcgaa cagctaatcg acgagttttt tgagattaca
     2461 cggtataacc tacaaacgat aacgctaaca aaaacgcaca tagacctata ctatatgctg
     2521 gtgcagatga ccgatgaatt ttatcctcag ctttccgcac atggaaaaca ggcggttatt
     2581 cacgcccccg aggatctgac cgtgtccggc gaccctgata aactcgcgag agtctttaac
     2641 aacattttga aaaacgccgc tgcatacagt gaggataaca gcatcattga cattaccgcg
     2701 ggcctctccg gggatgtggt gtcaatcgaa ttcaagaaca ctggaagcat cccaaaagat
     2761 aagctagctg ccatatttga aaagttctat aggctggaca atgctcgttc ttccgatacg
     2821 ggtggcgcgg gacttggatt ggcgattgca aaagaaatta ttgttcagca tggagggcag
     2881 atttacgcgg aaagcaatga taactatacg acgtttaggg tagagcttcc agcgatgcca
     2941 gacttggttg ataaaaggag gtcctaagag atgtatataa ttttttagga aaatctcaag
     3001 gttatcttta ctttttctta ggaaattaac aatttaatat taagaaacgg ctcgttctta
     3061 cacggtagac ttaataccgt aagaacgagc cgttttcgtt cttcagagaa agatttgaca
     3121 agattaccat tggcatcccc gttttatttg gtgcctttca cagaaagggt tggtcttaat
     3181 tatgaataac atcggcatta ctgtttatgg atgtgagcag gatgaggcag atgcattcca
     3241 tgctctttcg cctcgctttg gcgttatggc aacgataatt aacgccaacg tgtcggaatc
     3301 caacgccaaa tccgcgcctt tcaatcaatg tatcagtgtg ggacataaat cagagatttc
     3361 cgcctctatt cttcttgcgc tgaagagagc cggtgtgaaa tatatttcta cccgaagcat
     3421 cggctgcaat catatagata caactgctgc taagagaatg ggcatcactg tcgacaatgt
     3481 ggcgtactcg ccggatagcg ttgccgatta tactatgatg ctaattctta tggcagtacg
     3541 caacgtaaaa tcgattgtgc gctctgtgga aaaacatgat ttcaggttgg acagcgaccg
     3601 tggcaaggta ctcagcgaca tgacagttgg tgtggtggga acgggccaga taggcaaagc
     3661 ggttattgag cggctgcgag gatttggatg taaagtgttg gcttatagtc gcagccgaag
     3721 tatagaggta aactatgtac cgtttgatga gttgctgcaa aatagcgata tcgttacgct
     3781 tcatgtgccg ctcaatacgg atacgcacta tattatcagc cacgaacaaa tacagagaat
     3841 gaagcaagga gcatttctta tcaatactgg gcgcggtcca cttgtagata cctatgagtt
     3901 ggttaaagca ttagaaaacg ggaaactggg cggtgccgca ttggatgtat tggaaggaga
     3961 ggaagagttt ttctactctg attgcaccca aaaaccaatt gataatcaat ttttacttaa
     4021 acttcaaaga atgcctaacg tgataatcac accgcatacg gcctattata ccgagcaagc
     4081 gttgcgtgat accgttgaaa aaaccattaa aaactgtttg gattttgaaa ggagacagga
     4141 gcatgaatag aataaaagtt gcaatactgt ttgggggttg ctcagaggag catgacgtat
     4201 cggtaaaatc tgcaatagag atagccgcta acattaataa agaaaaatac gagccgttat
     4261 acattggaat tacgaaatct ggtgtatgga aaatgtgcga aaaaccttgc gcggaatggg
     4321 aaaacgacaa ttgctattca gctgtactct cgccggataa aaaaatgcac ggattacttg
     4381 ttaaaaagaa ccatgaatat gaaatcaacc atgttgatgt agcattttca gctttgcatg
     4441 gcaagtcagg tgaagatgga tccatacaag gtctgtttga attgtccggt atcccttttg
     4501 taggctgcga tattcaaagc tcagcaattt gtatggacaa atcgttgaca tacatcgttg
     4561 cgaaaaatgc tgggatagct actcccgcct tttgggttat taataaagat gataggccgg
     4621 tggcagctac gtttacctat cctgtttttg ttaagccggc gcgttcaggc tcatccttcg
     4681 gtgtgaaaaa agtcaatagc gcggacgaat tggactacgc aattgaatcg gcaagacaat
     4741 atgacagcaa aatcttaatt gagcaggctg tttcgggctg tgaggtcggt tgtgcggtat
     4801 tgggaaacag tgccgcgtta gttgttggcg aggtggacca aatcaggctg cagtacggaa
     4861 tctttcgtat tcatcaggaa gtcgagccgg aaaaaggctc tgaaaacgca gttataaccg
     4921 ttcccgcaga cctttcagca gaggagcgag gacggataca ggaaacggca aaaaaaatat
     4981 ataaagcgct cggctgtaga ggtctagccc gtgtggatat gtttttacaa gataacggcc
     5041 gcattgtact gaacgaagtc aatactctgc ccggtttcac gtcatacagt cgttatcccc
     5101 gtatgatggc cgctgcaggt attgcacttc ccgaactgat tgaccgcttg atcgtattag
     5161 cgttaaaggg gtgataagca tggaaatagg atttactttt ttagatgaaa tagtacacgg
     5221 tgttcgttgg gacgctaaat atgccacttg ggataatttc accggaaaac cggttgacgg
     5281 ttatgaagta aatcgcattg tagggacata cgagttggct gaatcgcttt tgaaggcaaa
     5341 agaactggct gctacccaag ggtacggatt gcttctatgg gacggttacc gtcctaagcg
     5401 tgctgtaaac tgttttatgc aatgggctgc acagccggaa aataacctga caaaggaaag
     5461 ttattatccc aatattgacc gaactgagat gatttcaaaa ggatacgtgg cttcaaaatc
     5521 aagccatagc cgcg
//
LOCUS       DAZUKZ010000140         5220 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000140 DAZUKZ010000000
VERSION     DAZUKZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.146"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1256)
                     /locus_tag="ACG8EF_002576"
     CDS             complement(<1..1256)
                     /locus_tag="ACG8EF_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS4947835.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="ACG8EF_002577"
     CDS             complement(1270..2487)
                     /locus_tag="ACG8EF_002577"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS4947836.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..4072)
                     /locus_tag="ACG8EF_002578"
     CDS             complement(2477..4072)
                     /locus_tag="ACG8EF_002578"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFS4947837.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            4197..4982
                     /locus_tag="ACG8EF_002579"
     CDS             4197..4982
                     /locus_tag="ACG8EF_002579"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HFS4947838.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            4975..5217
                     /locus_tag="ACG8EF_002580"
     CDS             4975..5217
                     /locus_tag="ACG8EF_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947839.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT         1574 a         1051 c          862 g         1733 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattgtggca atcaggtctt
     3841 cttttgattc ttcatctgaa agtaattcac gatacaacat tgtttgtttg cttactgtgt
     3901 catattcctc taaggattca tctgctaaaa gaaccacttg ttccaacaaa cggtcggata
     3961 aatatttata gaaaatcagt cccaataaat aatttttata ttctgacgca tccattttac
     4021 tacggagatt atctgccgca ctaaataatt tttgatttaa ttctgcaccc aaaaagatac
     4081 actcctatct cttatttaaa gttgctatat accctcttat tttagcatac tcccttttag
     4141 aaaaagcaaa aaaaaaagaa aaaatcttat tcttgataac aagctacgtg acgtatatgc
     4201 ctaacaaaaa tagaactttt gaatcaatct taaatgaagg tttaaccaca gaaccggcat
     4261 ccattatttt tgatgaagtt atcgatgacg ttgaagatag taagtatcat aaaaatttaa
     4321 aagaactttt ttattttgca ctcaacaatg gaccaagttt gttcactggt atatcagtac
     4381 ctgatagtga taatccatta attatggcaa aaatttactt aacaaaatgg tgtaataaat
     4441 acatcaggga taggaataac ccagcattaa aaaaacctct taaaacattt ggcgaaaaag
     4501 atgctgcttt aactactaga gtagcagcaa atgctaatat tgatgatcaa aaagtattga
     4561 atgactactt gcgtggacat tttttatata tgagcgctga gaacatgaac ggaagtattt
     4621 tagaggaata tttagccgaa gtgttagaac ctgaaggttg gatttggtgc gccggctctg
     4681 tatatcgagc tgttgatttc tgttatttag gaacctcacc aattctttta caagtgaaaa
     4741 ataaatacaa tacagaaagc agttcgagtt cagctataag agtaggaaca acaattagaa
     4801 aatggaatcg tttaaataag tcgacaaaaa tttctggttt ggatagccct atccccaatt
     4861 ggaaagcatt aatagaaatg actgaagcta gtaaggaact ggcagctaag ttaactgaaa
     4921 atagttattt agcttatatt aatgaaaaat ccacacgaga attatggaca ctcgatgact
     4981 aacaatatcc ttattagtat ctatccacga tacatggaaa aaattataaa caaacaaaaa
     5041 aactttgaat ttagaaattt ctatcttacc tcagatcctt ataatatctt ttggatttac
     5101 gaaacaatcc ccaccaaagc tttaaagtat aaattgattg taaaaaatcc aattactaaa
     5161 cttaagaaaa atcaacacta ctttttagga gacgatgagt ttgaacaatt agtttaaaag
//
LOCUS       DAZUKZ010000141         5154 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000141 DAZUKZ010000000
VERSION     DAZUKZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.147"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..649
                     /locus_tag="ACG8EF_002581"
     CDS             <1..649
                     /locus_tag="ACG8EF_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947840.1"
                     /translation="DTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLS
                     AVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMR
                     YQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITM
                     IHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="ACG8EF_002582"
     CDS             708..1148
                     /locus_tag="ACG8EF_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947841.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="ACG8EF_002583"
     CDS             1160..1828
                     /locus_tag="ACG8EF_002583"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS4947842.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="ACG8EF_002584"
     CDS             1892..2515
                     /locus_tag="ACG8EF_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFS4947843.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="ACG8EF_002585"
     CDS             2571..2957
                     /locus_tag="ACG8EF_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFS4947844.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="ACG8EF_002586"
     CDS             3170..4561
                     /locus_tag="ACG8EF_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFS4947845.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1708 a          861 c         1084 g         1501 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttaa gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt
     4861 caaaaaatag cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga
     4921 gaacaccgtt taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt
     4981 ccatataaat acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa
     5041 cttatttatt attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta
     5101 gattttccca ttttcctatg gtaaactgac aaagttgaga atgttagaac agat
//
LOCUS       DAZUKZ010000142         5091 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000142 DAZUKZ010000000
VERSION     DAZUKZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.148"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(130..1896)
                     /locus_tag="ACG8EF_002587"
     CDS             complement(130..1896)
                     /locus_tag="ACG8EF_002587"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4947846.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1856..3609)
                     /locus_tag="ACG8EF_002588"
                     /pseudo
     CDS             complement(1856..3609)
                     /locus_tag="ACG8EF_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(3719..4288)
                     /locus_tag="ACG8EF_002589"
     CDS             complement(3719..4288)
                     /locus_tag="ACG8EF_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFS4947847.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4306..>5091)
                     /locus_tag="ACG8EF_002590"
     CDS             complement(4306..>5091)
                     /locus_tag="ACG8EF_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4947848.1"
                     /translation="VLFVKNRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVS
                     ADVLKVEEITYENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIE
                     NKKNLSSPFLVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPF
                     DLSGGEKQRLLVSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATH
                     DPEFLYKTCNRIIYLKNGGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         1694 a          954 c          687 g         1756 t
ORIGIN      
        1 gtgaactaat tcaatcatac tgattctttc gtgtattcaa aattagatgc tgtttaataa
       61 aattattctt tttgaaaaag aattataact atatcccaaa aattgtaggt taatttaaag
      121 cctatttcat catcgtgatt gagcttcttt ttcatagacg aatttctcag atagtttagt
      181 tttctttatg agatttttgt atatttctga ccttcgatac aactcattat gagtaccttg
      241 atcaactagt tttccttgat ccatcactac aattttattt accttttcaa tagacttcaa
      301 gcgatgcgat ataattagta ctgtcttatc ttgtattaaa cgatttaaac tttcctgaat
      361 acgtttttca ttttccgcat ctaaactggc agtaatttca tctaaaatta aaattggcgc
      421 atttttcaaa aaagcccgtg ctatggatag gcgttgtcgc tctcctccag aaagtgaact
      481 tccgttttct ccgaccaatg tatgataacc gtcaggtagt ttttcaataa agtcttcgca
      541 gttagctaaa cgagctgctt ttttcacttc ctcatctgac gcttgactat ttccaatacg
      601 tatattttcc aaaatacttg tattaaacaa agttacctct tgaaaaacaa tagcgatttt
      661 gctaaacaaa gaagctggag atacacgttt aatatcatac ccatcaatta aaatacagcc
      721 ttcatcataa tcatacagtc tagacactaa tttcaagata ctagttttcc cggaaccact
      781 agcacctact aacgcagtca cttctccttg tttagccgta aaagagatat gatctaaaat
      841 tggggtattg ttatcatatg agaaagagac atctcgcaac tcaatgtcaa aagatttcag
      901 aggactatct gatccctctt ggatactggt ttccttcata gctctaattc gctgtatttt
      961 aggagctaaa taaaatattt caagcactgc ttctttcata gaatcgaagg aatcctttat
     1021 ttttattgcg gctagtaagt aaccaacgac gtagagtata gtcacttcac ccgttaacag
     1081 taagtgaaca cctactagaa tgactactgc taaactaaca tatgagaaaa tagaggataa
     1141 ggccatgact gaaaaagtac ttaattctac ctttaaatga atcctttcac tctcttccat
     1201 ttttttgaaa agtctgtctt gaactttttt agatagatta aagctattaa tctcttgctg
     1261 caattctata gtttcttgaa attcttccga gttttctctc aaagtatcgt aatatttagt
     1321 attggctttc gtttgggatt tctttgataa caagattaag acaaaactaa ataacgttgg
     1381 caaaataaca gctaatccca ttttgacatt accaaccaaa agcatcactg aaataaaagg
     1441 gaaaaacaga gccataccgc cggctttagg tattgcatga ctcatcgcat gctcaatacc
     1501 ttcaacgtca gacatgatag tttgagataa atctgataaa ttatgttttg aaaaatagga
     1561 taatggtaaa tttgacaaat cgtctgcaat ttgcactctt aaatgcgcac tttctttata
     1621 agtactatta tataagtttt catactccct atctaacaac caatacatga aaagcaaagt
     1681 aacaaacgat acggcaaaat aaagccaacg gggctttgta ttttctaaaa ctaattcgtc
     1741 tatcagcatc attataatga atgctggtac catattaatg cagtatacaa aaaaactaga
     1801 aatagaagct ttagttaaag cttctgcgcc ctgatcactt aaagcaaatt tgtttttata
     1861 aaaacttttc attgtcaagc ctccaatcat ttgcattctc ataattagtg accaattgtt
     1921 tatacacttg atgtttagcc attaattcat tatgatttcc acgttctact attttccctt
     1981 ctgacaagac aagaatttca tctacagatt taatgctgga taatcgatga gctatcataa
     2041 taactgtttt ttctttcatc agatgttgaa aagccttttg tagttcatac tcattatctg
     2101 gatcaataga tgcactggct tcatccataa taacaatctt tgcatctttt aaaattgctc
     2161 tggcaatagc tatacgttgt ttttctcctc ctgacagata aactccttta ctaccaatta
     2221 aggtatgctc tctttcggga aatttttcta aaatcgattc gcaaccggct aatcttagcg
     2281 cgtcaaatac tttacttgct ggcgcttgag gatttgctat agaaacgtta tcgtaaatac
     2341 tctgattgaa tagtttcgta tcttgaaaga caaaagcaat tgtgttaata attgtgtctt
     2401 ttgaatagtg atttaaaggc ttaccaccaa ttttaatctg tcccttatcc acattgtaga
     2461 aacctgaaat tagtttagct atcgtactct ttccgcttcc tgattttcct actaatgcat
     2521 aaattctttt ttgttctaaa actaagttga aattttctaa aacacactct tctttgtatg
     2581 aaaagctgac attttcaaat gcaatgtcac aatttttaat ctgtgtctcg ttcccatatt
     2641 ttagttggtc ttgcttcatt tctaaataaa gggagtctag tgtttcgact gcatatcttg
     2701 cttcaaacaa gtgcatactt acatacatta ttctcatgaa agaaacaaaa ataattccac
     2761 ttaaaaaaaa caacatcaac aagtcaacag ctaatatctt gggagtggta agagaagtta
     2821 taaaaaaggt tataggaact atcaaaatgc aaactatgcc aaaaaatagc cattgaaaaa
     2881 gaacaaaagg tgttttccca ctcaaagaat acttatatgc aaacttagca taatcgttaa
     2941 ttgctttatt caaggcttta aatgatgtta ctttagcacc aaatatcttt ataacttgaa
     3001 ttccgcggat atattctacc gtttcagagc ttaacacatc taacgattct tgatattttt
     3061 gaataaaatt tgtattctct gtcattttct ttaacaagaa tacaccaatt actaacatgg
     3121 cgattagaac caatccaatt ttaaagttaa tgaaaaagcc taaggcaact gataaaacag
     3181 gtgttactat ggcttgagca ctatctggaa tcaagtgagc aattgccata tgtgttttag
     3241 ccgcgttgtc atcaattgtc tttcgaatat atcccgaaga atttaaatca aaaaaacgaa
     3301 aacttgcttc tgttaaaccg ctaattccct tttttctcaa attagtctct aagcgaaacc
     3361 caaggatatg agaaaataag ccagaaaaaa tataaaataa agctctaaag ttaaccaagc
     3421 aacagtttga accgcataac tcttagctaa atattcatct cctacaataa ttagagactg
     3481 aagaaatttg taaatactgt aatatccata gactgttaat acgacagaaa aatcagaaaa
     3541 taaagtggct aaccatccgt agtatttcat tttagggaca taactaaata attttttgta
     3601 tactttcatt ttttcctccc ccgcattcaa tttcttctct tattcgttta caactgttaa
     3661 tgctttccgt aatagcgtaa attagagatg tacaaacaga taaaaggtaa actacgttct
     3721 attttttgta tttttttaag acttttcttc caatcaatac accaatcata gataaacttg
     3781 ctacaactaa cactgaaatg attccccata tacctaccgc atagtggatc attgcatcca
     3841 ttgttgcagc agattgtcct ttatctatcc aatcttgacg aaaacgatct gtaaagaacc
     3901 atataggaat ccactgtccg gcagcactgc ctaattgcat tagagcccaa gacgcaactt
     3961 gaggaattgc tcgatcatat ccaaatacag atagaataat atcagctaag atcgccatac
     4021 taatcgaact gataaaccaa ggtaagtacg ccccgcccat taacgtaaaa aataacaata
     4081 aaacaattgt gtaaatagta aaaattcctt ttctagggta gttatagcta atataaacaa
     4141 atatgattcc agataataat ccaaaaatcc cgggggatat gctgtgacca aacgccccac
     4201 caatactact aaccattatt gaaaaaaagt aaattactag ggaaataact gtagctaaac
     4261 caataaaaat ataatctttt gttttcatat atattctcct caattctatt tcgttatgtc
     4321 attaaaaatc tgttctatca aagttgaatc ttctactcta agttttatat ctttttttat
     4381 tccaccattt tttaaataaa taattctatt gcatgtttta taaagaaatt ccggatcatg
     4441 agtagctata actactgcac ccttttcttt taagtcttta ataaggtcag caatcctttc
     4501 catattaata taatccaacc cacttgtagg ttcatcaaat agaaataaat tagtttttga
     4561 caatgaagct atagaaacca ataatctttg tttttctcca ccggataaat caaacggtaa
     4621 ttgatctttc attttataaa gttccatttt ttttaaaatt ttttcttgct gatctttact
     4681 aactatttca ttccccaggg ctaactcgct cctcactgat tctgtgaaaa attggtaatc
     4741 catttcttgc attactaaaa acggcgatga caaatttttt ttattttcga ttatctttcc
     4801 tttggtaggg cgaataatct tcatgagtat tcttagtaag gttgttttcc ccacaccgtt
     4861 tttccctacc aaaccaataa catctccaac tttgacatca aaatttatgt cttttagaat
     4921 attttcataa gtaatttctt caacttttaa tacatctgca cttacctttt cgatataggg
     4981 aacttcaatc gaactattaa aaatgtcaaa tttccttaat gcgttattgt tttcagtttt
     5041 taacaactga atagttgtag tttttacagt tctattttta acaaatagaa c
//
LOCUS       DAZUKZ010000143         5061 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000143 DAZUKZ010000000
VERSION     DAZUKZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.149"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..660)
                     /locus_tag="ACG8EF_002591"
     CDS             complement(205..660)
                     /locus_tag="ACG8EF_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LMxysn_1693 family intestinal colonization
                     protein"
                     /protein_id="HFS4947849.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1063..1236)
                     /locus_tag="ACG8EF_002592"
     CDS             complement(1063..1236)
                     /locus_tag="ACG8EF_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947850.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYN
                     SPNEELFVKETDK"
     gene            complement(1917..2456)
                     /locus_tag="ACG8EF_002593"
     CDS             complement(1917..2456)
                     /locus_tag="ACG8EF_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS4947851.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            complement(2472..3395)
                     /locus_tag="ACG8EF_002594"
     CDS             complement(2472..3395)
                     /locus_tag="ACG8EF_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4947852.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            complement(4523..4678)
                     /locus_tag="ACG8EF_002595"
     CDS             complement(4523..4678)
                     /locus_tag="ACG8EF_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947853.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            complement(4697..>5061)
                     /locus_tag="ACG8EF_002596"
     CDS             complement(4697..>5061)
                     /locus_tag="ACG8EF_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFS4947854.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISI"
BASE COUNT         1607 a          838 c          771 g         1845 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag
      781 tagacataat gtatagtatg ccaagtagcc cttgtatcaa ccgatacagg ggctcacttt
      841 gtgattttta attggtctag gtaaaaagct ctttttaagg cttgattttc tgcagttgct
      901 tctttccgtt tctttgtgaa tttttaccag tcatttttta ctggattgtt atttttcata
      961 aaatatgata aatttttggt agcgctttat aagcgcgttg ctttttcaag aataaacact
     1021 cattattttt taactggctg ctcggaagag cagtcttttt tattatttat ccgtttcttt
     1081 tacaaacaat tcttcgttag gactgttgta ataatttgaa ttcaatttaa gttctctgtt
     1141 ggaattgttt tttccaatta cggataatag catggggcta aaacctaata ttcctagaga
     1201 aatagaaccc agtaccaaca cagttttttt taacataggt tatacctcca ctttttttaa
     1261 cgctaccact tgtatacaca ttttaaatac tagccttcag tttatcaaat gtttaaattg
     1321 aacggatata aatcaaaatt aaattttcaa aagttgttct aaaaaatttt tcttttttgg
     1381 aagttactct aacttttttt gcttgtggtg cttggtatgt tttctccgcc tcccaggttc
     1441 ttgaccattt tctcttataa gtgggatttt taccacgttg agagaaaatg gtcaagaacc
     1501 tgggaggcgg agaatgccct taataccaag caccgcaagc ctttcccata ctttattcga
     1561 tgatcccacg atgatctcat aatgattcta caatgaccct acgatgatca aaattctcaa
     1621 aaaaaataaa cgaaatttca aacgattttt ttgaaatttt gttaaaatca catttgtttt
     1681 tttatgactt tttcaacaaa atcgattaga atttattttg tttttccacc aaaaattgag
     1741 tgagatttca acctatttta agcaataagt caccatatag tttgatagtt tacaactata
     1801 aacattgatt gtttacagtt gtaaactatt ttttctttct cataaaaatt tgagtgatct
     1861 ccccatcagg tagtacaact ttttcctcta ctatccgaac taacttctag aaaattttag
     1921 agattggttc tattagattt ccaaatttgc caccactttt tttcaataag tggttgtgta
     1981 ttgtctgctt ttttttcaga aaaggcagtt gattcggttg ttggagtttc ttctgtataa
     2041 gttaaatgga gctgttcttg taggttctca atttgttggt tcgcttgtag agtgagttgt
     2101 tgttgctggt cgagtaattt ttgcatttgt tttatttgat tatctttttc ttgtagctgt
     2161 atatcttttc gatttaactg tttttctagg aaagaaagga gttttaattc ggaaacttgg
     2221 ttggtgtttt tatggttttt ggtttttgat ttttcgccaa ctagttttcg atttaagttt
     2281 gcgttatcga ctaaaaacat tgattttata gctttttgac cggtttcgtt aatcacaaac
     2341 ttgtttccgt tttttgcaaa ccaaagtttt cgatttttgt cagaaacttt tttgttaatt
     2401 gctgttttac taactcctaa ttcttccgca agttctttga ttgttttatc tgccataaat
     2461 actccttaag cttaatcgaa taagttttgt aaatttccta gtaaatcttg tttgagtttg
     2521 tttgaaagtt ctcgtttttc tgcctgtttt tcttcagttt gttgttgttg tttaaaatca
     2581 gcttcatgag tgcctaaggg aagatttagt attcgatctt tagcgcgcca tttttcttct
     2641 cgtgttaatt ctccattgtg atcaatatta aataatttta tacgtaaatc ttcttgtttc
     2701 cctttggaaa aatcgtcagc atggttaatt tcagctttcc aagtaaaacg ataaccgata
     2761 acaggtttac ctcgaccttt accatatttt ttacgaatag ttaatccttt aaataaggga
     2821 gtgagttctt ctttaattgg ccttaagata aatttgtcaa tatttgacgg actattccaa
     2881 taacttttag gaatatctag taaatcaaaa aatttttctt tggaaaattc agtccagcct
     2941 tgagttcgat attgttttaa taatcgaaac atagtttttg aatatgtgct atccaaatta
     3001 gtaaattgct ctagattaaa tcttgtccag ttctttaatt gattaaaaag tcctttaaat
     3061 tctggattaa ctgaaattat aacaagttgt ttatctcgta taatgctata ttttgtgaaa
     3121 gcaacaaatt tagttaaagt atttccatca tcattccaag cattaatact taataatttg
     3181 tcatacgtgc tactaagatc ttttataaat tgttccccac gttctgtata accgcttagt
     3241 tttttcaaat cgtgaaatga aaaaataatc tcatctacac ctttttcacg tacacgtgaa
     3301 gcaattgaaa aaaataagtt catttcacga ctattaaatt ttctcagagg aatatgattt
     3361 aatctatttt catattttac aatttcattt gccataagct tctcctactt ttataaagta
     3421 tgaaataaaa gtaacatact ttataaaaat actcaataat gtcggaaaac ctacttgaaa
     3481 agcgtagaaa acctacttga aaagcgtaga aaacctactt gaaaagcgta gaaaacctac
     3541 ttgaaaaaac tctgaattgc ttgggagagt aaggacacag ccgacataaa agagatataa
     3601 aaagagatat aaaaagagat ataaaaagag gggaggctaa ttttcccgcc taaaaaatca
     3661 ctcctttttt ctctaaacag gttcgcgaat ttatcaaaac tgccaattcc aaaccttgct
     3721 ccctacggtt cgctaaaaag gtgtgatcgc tctgctctca cattatactg cgcagtcgcc
     3781 tccggctcct ttgctaaaag ccgttcataa ggaacccttt gagaagctct gagcgttcgt
     3841 ttaagacatt ttaagacagt cctagtataa atcccttatc ttgttcttaa aacgccttac
     3901 aggcggtcag ggcaagccct aacaacccta aaattgtcta aatgccctct aaaattcgat
     3961 ttaagcactc ttaaacctat tcttatatat ttatatactt aggcgtttca aacgtcttaa
     4021 aacgcaaatt agagcttgat aaagatattt gctcgtgtgc ctagactttt aaaaaagtcg
     4081 gcacacgcca tgacaaagct gaaataaact gcttcagctt tgtcatggcg gattcgctca
     4141 cttgaaaaag tgtttatttc gctcgattat tagcatatat tttttagctt ttttgctagc
     4201 caaattttgc attaatttgc tcataagtcc caccctttca gtacgtccta cggacgtatc
     4261 gttcgtaatg tgaaagtacc tttaaaggta ctttctacaa aactcactaa aagggtttgg
     4321 caagctctat atttgctagc caaataccta cgtatttggt gcaaataacc gcttgtcaaa
     4381 ggggcgcaag ctcccccttg aatcccccct caaaacccac gctccacaat acaaaaaagg
     4441 ctagcaaatg ctagccactt tttgtaaagt ttcgcttgtt tcaccttcgg tgaaattgtt
     4501 gctttgcaac gatcaggtag agctacattt tttttcgttg ttcaaatatt cttacgccat
     4561 ttactattaa aatcgtaggt attccaaagt aaagaaaaaa ttgattagca ggtaatacaa
     4621 aatataaaat aataccagca ataattacca aacttatagt aagttttgtt aatttcatga
     4681 agtatgccac cttatcttat atagatatca aatagattaa tatgccgatt gtattataaa
     4741 atccatgagc aactatggaa taccatatat tttttgtttg tatataaaca aaacttaaaa
     4801 taataccaat aaagaaaata cttaggcctc ccccatgaac aatagaaaac agaaaagaag
     4861 aaattagtat tgaaatatat tgttgttttt tgtttgcttg aggatcaatc atacaaaatc
     4921 gaaaaattcc ttcttctaaa aaagctccat aagtagcagc atttaaaaag aataaaagga
     4981 gagaaccatt tacttcatta ggatttatac cttctgaaaa agatgtattt agaatattga
     5041 ttaggaataa aacagagaat g
//
LOCUS       DAZUKZ010000144         5043 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000144 DAZUKZ010000000
VERSION     DAZUKZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.150"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..807
                     /locus_tag="ACG8EF_002597"
     CDS             91..807
                     /locus_tag="ACG8EF_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFS4947855.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            826..2409
                     /locus_tag="ACG8EF_002598"
     CDS             826..2409
                     /locus_tag="ACG8EF_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HFS4947856.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            2538..3122
                     /locus_tag="ACG8EF_002599"
                     /pseudo
     CDS             2538..3122
                     /locus_tag="ACG8EF_002599"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            3068..3586
                     /locus_tag="ACG8EF_002600"
     CDS             3068..3586
                     /locus_tag="ACG8EF_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947857.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            3609..4634
                     /locus_tag="ACG8EF_002601"
     CDS             3609..4634
                     /locus_tag="ACG8EF_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS4947858.1"
                     /translation="MKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVAVSLAI
                     FGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQRPSKVI
                     YDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAKKRGIT
                     ISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINSGELNK
                     DGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYNIQIVD
                     RVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYNLMNSG
                     GNGRVLR"
     gene            4883..>5043
                     /locus_tag="ACG8EF_002602"
     CDS             4883..>5043
                     /locus_tag="ACG8EF_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4947859.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         1866 a          575 c          986 g         1616 t
ORIGIN      
        1 ttatagagga agaaacatta tttcaatgat taagagtcga gagcacaaac ggtagacgat
       61 gttattttta taaaaagata taatttatta atggtcgata attatgaaag gaaaaatatt
      121 cttaaaaaaa agagtatagg atgtcgtatg aaaatagtcg ctatagtacc aatgaagtta
      181 aataacaaaa gattgccaca aaaaaacacg aaagctttta ccaatggaaa accattgtgt
      241 tattatatac tttcaacact tttaaaagta aaacagattg ataaagtgta tgtctattgt
      301 agtaacccag atattaaaga atttatacca gaaggtgtaa catttttaaa gagatctagt
      361 tccttagatc aggatacaac gagtattaca caggtattag ccggtttcac aaaagaagta
      421 gaggctgatg tatatgtaat gacacacaca acagcaccat ttattagcga aaaatctatt
      481 gaaaaaggac tagatgccgt gctgacagga gaatatgatt ctgcatttgc tgcaaaaaaa
      541 ttgcaagatt tcttatggaa agacggtgaa ccatttaatt atgaattgga caatattcct
      601 agaacacagg atttgccgcc tttatacgaa gaaactagtg gattttatat ttataatcgc
      661 aatgtgatga ccgagttgca tagaagaatt gggaaaaaac cttgcatagt tgaagtaggt
      721 gagattgagt cagttgatat tgatgaagcc gaggatttta tgattgctga tgcgatttat
      781 aatcatctta ttttacaagg tagttaagac agggggaagg ttaggatgaa caatgtacag
      841 attttagatt gcacacttag agatgggggc tattgtaaca attgggaatt tggatttgat
      901 aatacaaaaa aaattatttc tggacttata gaatcaggaa ttgagattat agaatgtggg
      961 tttctcacaa ataaaacagt atacaataaa aatgttacaa aatatacgac cttagaagaa
     1021 ttagcacaga taattcctga aaaaagagaa ggaaaattat ttgtagcaat gatgaactat
     1081 ggcgaatata atatagagga tcttccagat tatgatggtt catctattga tggaattagg
     1141 attgcgtttc ataaaaaaaa ttgcaatgaa gcgttgaaat tatgtcgtga gataaagaaa
     1201 aaaggctact tagtttttgt tcaagctatg gtatctctca attatacaga tgaagagttt
     1261 ttagcagtaa taaaaaatgt gaatcagttt aaaccatacg ctttttatat tgttgatagt
     1321 tttggaatga tgaaaggaaa agatttaact cgtctttttt acatgattga gcataatcta
     1381 gaaaaagata tacagatagg ttttcactca cataataaca tgcagcttgc atattctaat
     1441 gcacaaaaac ttataaccgt acaaacaaat cgaagcctga ttattgattc ttcggtgtac
     1501 ggaatgggaa gaggagcagg taatcttaac acagaactat ttgtagaata tttgaatgat
     1561 aatgcaggaa aaaaatatca attaaaacca ttattatata taattgatga aatactaaat
     1621 agtttctatc agaagagtta ttggggatat tcattaccaa actatatttc agcagttcat
     1681 aatgctcatc ctaattatgc aagctatctg tcatataaga atactttaac aattgaagca
     1741 atgaatgaaa tattcgaaat gatggatgaa gaaaaaaagg tatcttatga taaacaatat
     1801 atagaagatt tatactacaa atatatggaa actgggaaaa tacaggagga atatatggaa
     1861 gaactgagaa aatttctctc agacaaaaaa attctactga ttgcaccagg aaaaagttct
     1921 gtagatgagg tagaaagaat aaaagagttt gttaccaaag aagaagtcgt aataattagt
     1981 gtaaattttg aatattctca tatagatata gattacgtat ttttaagtaa tttaagacgt
     2041 tttcatgaat taccgaaaga aagcagaact aaatgtattg ttacatcaaa tatacaagca
     2101 tcggatgtat atttacggac gagttataaa aagttgttat ctgaaaggga agttgtaaga
     2161 gacaatgcag gattaatggc aattagattt ttagccgata tgaatgtgga tgaaatattc
     2221 ttagctggat ttgatggata ttcacataat gaagatgaga attatggtga gcaatccatg
     2281 gaaattataa caaaatttgc attattagat gcaatgaacg tgcaaatgag agatgaattg
     2341 tcagaattag ctaaaaaagt aaaggtgaca tttttaacgg atccacgttg ggttcgaatt
     2401 gataagtagt agtaataatg tttttaaaat tttgataaag agatagtgag agaataaaaa
     2461 tgggtaaagt cttaaataaa aaagaagaaa aatatttaag ggacgatggg gcgagatgaa
     2521 actctaattt tgaactttta agaatcattt ctatattgtg tattgttgct taccatgcta
     2581 atttgcgttt aaattataat gcagacggaa ttatagtaca tggttcatta actgctaacc
     2641 agattttctt gaattatatt ggtatttggg gtcagctagg agttaattgt tttatattta
     2701 tttcggcata ttttttaagt gagaaaagaa acattcactt tgaaaatgtt ttgggaattg
     2761 tattagaatg tattttttat agtgtattag tttatggaat tctttgtgca tgtggaatag
     2821 aaacattttc attgaacagc tttgtaaaag aaatattatc atttgctcta ggtagatatt
     2881 ggtttattac atcctattta gcattgtata ttctaagtcc gtatttaaat atcatgctta
     2941 acaatctgga aaaacttcag ttgcaaaaaa taagtatgtt attgtttata ggtgtatgct
     3001 gttatcgtat gttatttaaa acatctttgt ttggggattg tgctgtttta attgcactat
     3061 atgtcatatg ttatacactg aaaaaaacga taataatatt tttgagagac acagaatcat
     3121 aggaacaatt gtatgtataa cagttatacc acttctcata tccataggaa tacttttcaa
     3181 atttatacca aatgcaatgg ttaaccagat ggttcatatt ggttctccat tgatggttat
     3241 agcaagtata tttttatttt atgtatttaa aaatttttca ttttacagta agtatataaa
     3301 taaacttgcg agaaccagtt taggggtata tattttacat gaaaatcatt atttcattga
     3361 tggtatgaag tctagaggct taatatggga tgggatattt ggattaggca aaaggttttt
     3421 agatagggat ttttggattt ggtttataat tgcagttgta gttgtgtgca taatttgcat
     3481 ggctatcgat gttgtaagag aaaaatttga aagacagatt tttaaaatta agagtgtaaa
     3541 gaacctgtgt aaacttatga atcatgttat aaatatatac ccataattaa taaatgagga
     3601 gaaaagaagt gaaagttata tcattcggag aattattatt gagacttgca gcacccggtt
     3661 attcaaagtt atttcaaaga gatagtttgg agactacatt ctgcggaggg gaagctaatg
     3721 tagcggtatc cttagcaata tttggatatc aatcagaatt tataacaaag cttccggatt
     3781 cagatgttgg atatgcggca gctaatgctt tgcgttattt tggagtaaat gttgataata
     3841 ttatatttgg agagggacgc atgggactgt attatttaga aaaaggagca gctcagagac
     3901 catctaaagt aatttatgac agaaaatttt ctgcaatttc gttagctgat agtgatgaat
     3961 ttaagtggaa ggagatccta aacgatgcaa attggtttca ttggacaggg ataaatccag
     4021 cattgtctga gaatctcttt gagacgtcct tagatgcatg tcaggctgcc aaaaaaagag
     4081 gtataacaat ttcttgtgat ttaaattatc gatccaagct ttggacatca gaggaagcaa
     4141 aagaaaagat ggaacagata ctaccattgg ttgatatttg tatttgtaat gaagaagatg
     4201 cagaaaaagt gctaggaatt aaagctgagc atacaaatat taatagtgga gaattaaata
     4261 aagatggcta tgtgcaggtg gctagggaga ttcacaagaa gtttggatgt aggtatgtag
     4321 ctacaactct gcgtagaagt tattcggcaa gtagaaatgg ttggagtgca atgttatacg
     4381 aagcagaaaa agataaagcg tatttttcca cagaatacaa catacaaatt gttgatcgcg
     4441 ttggtggtgg agacagcttt tctgctggta taatatatgc gttatgtgaa aatatgtccg
     4501 gacaaaaggc tattgaattt gcgactgcat ctagttgttt aaaacatact atagaaggtg
     4561 attttaatcg aactacaatt gaggatgtat ataatttaat gaatagcggt ggaaatggtc
     4621 gcgtgttgcg ttagtcaaca tataaagata aatcacagta atttttatgg tgattatctg
     4681 tcttagtagt gattagaata acgtgaaaat aaattgttga gcgtttatac tgttttgaaa
     4741 aataaattta acatattgaa aaatacaaat atatacgtat attataaagc gccgattgta
     4801 aaattaagct agacaactaa aacagtcatt tgtgctacac tcaaaatagt tccgaccaaa
     4861 gaattataag gagtgagtgc aaataaccta tacctatctt acaacggatg aacttgtaat
     4921 aatagagtct tatttgaaaa ttaaccaatc tgttgctaaa actgcgcatt gcttgaatcg
     4981 ttcaagtcaa acgatccata aagtatatct gttcttcaag caaagaaaat cagccttaga
     5041 ata
//
LOCUS       DAZUKZ010000145         4993 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000145 DAZUKZ010000000
VERSION     DAZUKZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.151"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(121..1269)
                     /gene="nagA"
                     /locus_tag="ACG8EF_002603"
     CDS             complement(121..1269)
                     /gene="nagA"
                     /locus_tag="ACG8EF_002603"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS4947860.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1345..2181)
                     /locus_tag="ACG8EF_002604"
     CDS             complement(1345..2181)
                     /locus_tag="ACG8EF_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4947861.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2365..3582)
                     /locus_tag="ACG8EF_002605"
     CDS             complement(2365..3582)
                     /locus_tag="ACG8EF_002605"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4947862.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3731..3892)
                     /locus_tag="ACG8EF_002606"
     CDS             complement(3731..3892)
                     /locus_tag="ACG8EF_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947863.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3965..4048
                     /locus_tag="ACG8EF_002607"
     tRNA            3965..4048
                     /locus_tag="ACG8EF_002607"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3999..4001,aa:Leu,seq:gag)
     gene            complement(4288..>4993)
                     /locus_tag="ACG8EF_002608"
     CDS             complement(4288..>4993)
                     /locus_tag="ACG8EF_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947864.1"
                     /translation="TPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMN
                     ILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMI
                     WSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVIL
                     MIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFG
                     LTVLGICTFIITVINR"
BASE COUNT         1536 a         1026 c          801 g         1630 t
ORIGIN      
        1 attgaacaat aggaaattaa agaaactttt gaaacttatg taaagtgatt tttactagaa
       61 cacaaaaaaa ccttaaaacc aaacttaaga agtttagttt taaggttaac ttaaattata
      121 ttatgcgtgg aaacgttcca cgccatctag ataagttgct tccaatgtca tatcagggtt
      181 caatacgata aagtctgcat cgcgtccttg agcgattttt ccacatgtat catcgatttt
      241 acagctgatt gctggaatca gactagccat catgactgct tgttctggtg ttgctaatcc
      301 ccagtctaca acatttttaa ttgcttcttt caatttcaat atacttccag ctaagttgcc
      361 agattctaga cgtgctgttc cttcttttac tactactgga aattcgccta aattgtaatt
      421 tccatcaggc atcccaccgg ccatcataca gtctgtaatt aatgcgacat gatcatgtcc
      481 tgctttttcc attaggattc ccgcagcatt tggatgtaca tgatggccat cacaaatcaa
      541 ttcagaaaag acatgttcta aagataataa cgcacccacc atacctggtt cgcgatgatt
      601 caatccacgc ataccattga aagcatggac aaatacactt gcgcctgctt caacagcttt
      661 ttgcgcttct tctagtgtag cattactgtg tcctagagaa acaactactc cttcttcagt
      721 cacttgtttg acaaactctt ctacaccttg acgttccggt gctaaagcaa tttttttgat
      781 taaaccacct gaagcttctt gccattcatg gaaagtatcc aaatcaggat ccccgaaata
      841 acttgggttt tgtgcccctt tgtgttcttc tgtgaagaaa ggaccttcaa aatagattcc
      901 tttgatttta gctcctggtg cttcctgata cattttgccg attgtctcag ctacatcttt
      961 taaacgttct ttgcttgacg tcaatgttgt tggtaaaaat gaagtcactc cacaagataa
     1021 cagaccttca gacatcactt tgatgccttc tgcatcatta tccattacat catgattcat
     1081 atagccgtga atatgtgtat ctaccaaacc aggagcaatc catttgccgc tttggtcaat
     1141 gatgttgact tctccctcag gtagttcttt ggtgtattca ccaaaaagtc catcggtaat
     1201 ttcaagatag cctgcatgtt tgacacctga cgctaaaaag aatttgtcag catagataaa
     1261 agttctcatt ctattctctc cctttgtttt tctacctcta aagtactctt atttagagaa
     1321 gaagtcaaga atggtctaca ccatttacaa attttttgaa atttgtttca cagcttgtgc
     1381 taagccttca tagaatgctg ctctttctgg ttcttcttta cgcttgtata ttaagctttt
     1441 aagcacaaat aaatcattga ggtaatagta gctattcttc tcttgagccc aatagattcc
     1501 ttgattcacc tgttctaacg cttcgtcgat ttcattttga tggaataaga aatttccata
     1561 attataaaaa atttgcgaaa attcactttt tgatcgttca tcagctgatt gatgcagcag
     1621 ttggatgctt cgactcaaat agtagcgtgc ttcagccgtt tttcctgcca caccatatat
     1681 ttttccgata caactaatca gttgtatttc tgtgtcagaa acatggattc tttctttttg
     1741 gctagagtag cttaatgctt ctttcaaata ataaagggac tgagagatat tttgacgcaa
     1801 cgtgaattcg caaacaccga gataataata atagcgttga aagtcagttg cttgatacag
     1861 attttcagaa atatcagaag attttagtac ttgttctaac ttccgataat cacgtttttg
     1921 gaaacaacta tccaacagtt caaataattt atcgtttgtc cgagtagttt ttcgagagag
     1981 attcatcaca tgatcaatcg ttacccctag ccgttgacaa atctgcatca tcaccaatac
     2041 attcggcagt tcctcattat tttcgatccg acttaataca ctttgtgaac aaatatcatg
     2101 cgaaagcatt ttttgcgtca ttttttgttc ttttcgaatc tctttaatga tcgttccaaa
     2161 ggactcgatt aacgtttcca taagtatcct ccccaaaata tgcgtattcg cattctattc
     2221 gtattttact ttaggaagtc gcaaacttca agaaaagact tgcaactaaa taatatactt
     2281 tagttattta agaaaactcc actagaaaga gagcctcagt gtgtttgtct gtggccctcc
     2341 tactgaaaaa tttggttttt aaaatcattt ttggcgatag gttgctagga attctctcat
     2401 tttcttagct gtttgtttca aatcatccat tttaggaaga taaacgattc gaaaatgatc
     2461 tggctggttc caattaaatc caccaccatg tacaagtaga atatgatgtt catgtaaaaa
     2521 gtccaaaacg aatttttcat catccaaaat attgaatcgc tttgtatcaa tttttgggaa
     2581 tatataaaaa gcagcttttg gttttacggc tgaaagacca ggaatatcat tgattgcatt
     2641 atagatatat tctctttgtt catagatcct tccgcctggc aggagcagct catcgacact
     2701 ttgataaccg cctagcgcgg tttggataat ttgctgagac aatacattgg aacacaaacg
     2761 catggaggac aacatattca gcccttctat atagcctttg acgtgtgact tatctccact
     2821 taggaccatc cagcctactc gaaatcctgc tacacgatga gatttggata gaccgttcag
     2881 cgtaacaacg aacaaatcag gcgctaatgt agcaatcgga atatgttcta gaccatccat
     2941 cactaagcga tcatagattt cgtcggaata aataatcagg ttattttgtc gagctacttc
     3001 aacgatttct tctaataatt cttttggata aagtgcccca gtaggattat taggattaat
     3061 gataacaata gcttttgtcc gactcgttat ttttgatttg atatcatcaa tatcaggata
     3121 ccattctgcc tcttcatcgc aaatataatg aacaggtgtg cctccagcta atgcaataga
     3181 agccgtccac aacggatagt ccggcattgg aaccagtact tcatctccat tgtccagcaa
     3241 cccctgcata cacatcgtga tcaattcgct cactccgttg ccggtgtaga tatcattgat
     3301 cgtcacattc ggaaaatgtt tcagttggca atattgctca attgcttttc tagcagaaaa
     3361 gatccccttg gaatctgagt agccttctga gtcacgggca ttcatgatca aatcacgaat
     3421 gacttcgttt ggtgcttcaa atccaaaagg tgcgggattt cccgtgttca attttaaaat
     3481 actaatgccc tcttcttgca tccgctccgc ttcttctaga acgggaccgc gaacatcgta
     3541 actgacacct tctaatttgt tggatttttc aaattctctc atcacttagc caatccctca
     3601 tttcaaaaag ttagaacttt ccaaattttt tgttatttac tatttcaata tcatactcca
     3661 tcctagaaaa ttttgccact tatttaggaa aaacgtaagt attaaaaaaa cacaaacaag
     3721 cgttgagaac ttaattgtca tgatagtcat tccatagatt cgctgcccaa tccattgcaa
     3781 ttccacaaat gattcctagc ataaaaccaa gcaaaaaacg tccgaatgca agtccaatca
     3841 atacacctaa gatcattccc agtcctgaaa attctccgtc atatttctcc atcttaataa
     3901 gtcctcccat ttttgagctt gacaactatg taaaacctcg ctatcattag tgtgttatta
     3961 ttacgcagat gtgttggaat tggcagacaa gcatgattga gggtcatgtg agcgttagct
     4021 cgtgtgggtt cgagtcccat catctgcact acaactcgtg aagttaattt tgcttcacga
     4081 gtttttttgt ttgaacgttt ctttatgcca ttatatacat atcactgtat cctttacaaa
     4141 aagaacgtac aatcatgtaa acagcctgag gtgagtatat gaaggtcgga aacaagctac
     4201 tcgtagaaac ttgcaagtgt taaaacaatt aatcgaaaaa cgagtaaaat ccctcaccta
     4261 atactaaagc gagggatttt ttgtatatta tctgtttatc accgttatta taaaggtaca
     4321 aataccaagc acggttaatc caaattgcat aaattcattt ttttgtacac tcatgctttt
     4381 cattagctct ttaactgcca ttagtactaa gatggcgata acaacataaa gtatatagta
     4441 cgtccgcatt ccggaagccc atactgtggg agaaaatccc ataattaacc ttgaacaaaa
     4501 tcccattatc atcaaaataa ctaaaaaaat agaagttttc ttattgtcaa aacttaaata
     4561 aattccaaca attaaacata gtaataatgc caagactact aaagtagcta tccaagtacc
     4621 tggataaaaa agtgacagtt tcgttcctaa ctctgtaaag agattattca ggttactact
     4681 actccaaatc attgcccttt tatttccatt gacgtaagta aagctttgac ccattgtatt
     4741 accaaaataa ataatcaggt ttaagaaaaa cggtaatgca gtcaatatac gcacataata
     4801 gttttgacat ttcctatatg ttagaaggaa aattaagaaa aatagcaata aaaataagat
     4861 attcatgtct aaaaaaaacg gctttccaaa tgatgaaata cctaaatcta acttattaag
     4921 aaagttgaaa tttttatatt caggaaacca tttattaatt tcatgtgcag cacgtaatgc
     4981 attccctgga gtt
//
LOCUS       DAZUKZ010000146         4961 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000146 DAZUKZ010000000
VERSION     DAZUKZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.152"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..1338)
                     /locus_tag="ACG8EF_002609"
     CDS             complement(139..1338)
                     /locus_tag="ACG8EF_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4947865.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1468..2844)
                     /locus_tag="ACG8EF_002610"
     CDS             complement(1468..2844)
                     /locus_tag="ACG8EF_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4947866.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2844..3500)
                     /locus_tag="ACG8EF_002611"
     CDS             complement(2844..3500)
                     /locus_tag="ACG8EF_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4947867.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3510..4787)
                     /locus_tag="ACG8EF_002612"
     CDS             complement(3510..4787)
                     /locus_tag="ACG8EF_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4947868.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4806..>4961)
                     /locus_tag="ACG8EF_002613"
     CDS             complement(4806..>4961)
                     /locus_tag="ACG8EF_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFS4947869.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1556 a          904 c          607 g         1894 t
ORIGIN      
        1 tcggagtaag tggcttcgac gattggataa ataaattcaa aatcaagcgc ggcatcaatc
       61 tttcgaacga gatgatcttg aggaactaga tcatcaatcg taagcatcgc aaattgattt
      121 ctaccattcg tattgttttt agacatcata gctatcactc cttgcattcg ttttcataaa
      181 tctattataa aagaaaagac tgtagacatc atccataaaa tggactttgt ctacagtctg
      241 cgcattctca ttagagaaca ctcccttttt tgtgactttt ctgaagctag agtgaatact
      301 ttcaactgca ttagtagtgt acatgatttt tctaatggcg ctgccataat caaagagttg
      361 ttcaacatga gaaaagtttc gtttgcatac atctactgca ccagaataat gagaccaccg
      421 attttgaaaa ctgtcgaagg cagcgtgtgc agcgtttaaa gaagaagcac tgtagaattt
      481 tttcatgtct cgacagactt ccttataatc cttacttggg aggtatcgta acgcatttcg
      541 aacaagatga acaatacagc gctgcacaat gacagaagga aaaattgctt ttgcgccttc
      601 tttgagaccg gaaacaccgt ccatcgaaat gaaaaaaaca tcttctacac cacctacttt
      661 cagttcatca aatacttgca tccagcgatt tttagattct gtttgattga gccataggcc
      721 taaaatctcc ttgtttcctt taagatcata gccaagaatg gtgtacacag catattcttt
      781 agcttcatag ttttcgcgta aagtgacgta catacaatca acgaatagaa atgcataaca
      841 cttggctaat ggccgagctt gccattcttc caattcagga aggacggcat ccgtgatatc
      901 agaaatcatt tcgtgggaaa tatcaaagcc gtaaatatct tcaacggttg cggcgatatc
      961 gcattgactc attcctcgtg catacatgga aagaacctta ccttcgatgt cggagacatc
     1021 tcgttttcta ttaggaatca actcaggttc aaaagaagcc tcccggtctc tgggaacatc
     1081 aatgtttact ttaccaaagc tcgttttaag cgtcttattg ccataaccgt ttcgtcgatt
     1141 gtcatgtttc tttggctctt tagaatgggc gtcataccct aaatggttat tcaattctcc
     1201 ttgaagcatt ttttcaaata agggaccaaa cacatctttt aaaacatctt gcatatcctc
     1261 tacagattcg ggttgataag tattcaaaat ggattcagct aacttctcag catcaggatt
     1321 tcttttcttt ctagccatcg tgtaatcacc tcttgatctt attttagaaa aataaaaacc
     1381 gtgaagcaac cacccgccta ggattgatgg ttacttacac ggtttatatt acactctcat
     1441 ttttggtgct aaaaagcacc gaaaatttta attattgtca attaataatt cttttggatt
     1501 ccttcttaat atattgaatg aagaaataat caatgataca agaagtacta aactcataaa
     1561 tataactaca taaatcatga atttaggtaa tacattgata tctaaacttg ataatgtctt
     1621 gttaaatcct tcagcttctg ccccaccacc taattggcta gatgcagatt gtttagctat
     1681 ttgtttagct atatcaccag taaccttatt caatatatta ttcccaagct tgtcagctgt
     1741 atattgagct aagaaatagg aaccaacgaa tgcggggatt gatataaata ccatctcaat
     1801 taggaattga ccaaaaattt ctaattttga tatacctaat gatagtaata ctgctatttc
     1861 tttctttcta gcattcatcc ataagaataa taataatgaa actacaactc ctgcaaatat
     1921 taatgagcca aaaaataatt tatttgaaat tgagtaaata cctgatatag attgttgtaa
     1981 tgcagggtaa tttgatgaac ttttaatcaa attatattct ctccaattta tatctaattt
     2041 cccaagatct tttattacac tatcaagatt cttatcacct tttacaaaga atgttgcatc
     2101 ttggtataca gctgtatctt cggtattacc ataaacttta gcagctgtat gaatatcagt
     2161 gattaatgta ttttcgtata gttcttgtgc tgcacttact ccaccgctat tatgcccatc
     2221 gaatagacct ttaatttcta cttctactgt ttcatctgca cctttctcat tatcagcatc
     2281 aaataaatta gattttattt ttatcttatc tccaacttta aggttatttt tttcagctaa
     2341 atctttatgc attaagattt tatttttatc ttcattttct aaatgctttc cttctactaa
     2401 tttatatgct tctgatacaa attttgtttc ttttgttgag tcgttaaccc cagttaacat
     2461 aactgttctt ttaaaattct ttgctctttc aggtgattga ttcgcaagag tctcttgagt
     2521 ttcaataata tcataatcaa ctaaatctgc aacactgttt attcttttta cataggagtc
     2581 tatactatct gtttgagata tctttttaat atcttcacct tttacatttc ccccacctct
     2641 aggtgttcct ggatttactt gtctatttat ctccatagaa aaactatttg ttatattagc
     2701 aaatgtttct tttgaagctc tgtccgtagc atctttaata gataaactaa tgagacttaa
     2761 agttgacata gataaaataa ctaatatgat aattaatgat tttaaacttt ttctagttac
     2821 ataagcaaat gcatttttta tcattattca acctccaaat tcattttatt tacttttttc
     2881 aactttttac cacttagttc taaaatgata tccgcagaat ctgctacttc cttactatgt
     2941 gttacaacta ttacacattt atttctatct ttagctaatg tctttaatat attaattatt
     3001 tctccagcag taacactgtc tagattacca gtaggctcat cagctagtat tattggagca
     3061 tctgatacca atgctctagc aatagctact ctttgttgct gtccaccaga taatttcata
     3121 acatttcttt ttatttgttt tttatctaaa cctagttcaa acaagatact ttcatctgct
     3181 gatttattta ctaatctaat attttcaatt ggtgataaat aatctattaa attatagttt
     3241 tgaaatacta aagatatatt attttttcta tgattactat atcctttatt ttgtatatct
     3301 tcattcttaa acaatatttt tcctgtttga ggtttatcaa gtccagcaag taaggaaaga
     3361 agtgtagatt ttcctgttcc tgactttcct actatcgcat aaaacttccc aagttcaaat
     3421 ttttgattta ctcctgacaa aactttttct ttagaatttg cataactata tgctacattc
     3481 tttatttcta atatatccat tattttctcc taacttattt ttgataagat ttctttaggc
     3541 ttcttgatta acattaatga agaagcaaat acaactgata aaacaataat acttattaat
     3601 attaaataac tttgtccaag tgttgttata tttgacataa aactactatt atttattaaa
     3661 cttccaccgg aaatcattga atcctctgag ttaatgaatc catctacaat tacttttagt
     3721 aatacatttc ctaaaaataa ggaagatatt atacttggta tcgatatgaa tattaactcg
     3781 aatataaatt gcattataat ttgtatctta gatgttccaa tagataagaa tatacctatt
     3841 tcataaattc tttcccttaa ccatagaatc aagattaatg aaagaacaac catcccacct
     3901 aacataatag aataagtcat tacttttatt atatatttta ttccactcac tgactctaaa
     3961 gactcttcaa acgcattaga atctttttca acaaaatact ttgactcatc aattttaagc
     4021 tctttcaatt tgtttaaggc taagtctgta gattctgcac taccagaata cattaaaatt
     4081 ttatttgcaa tttcattatt ttctgaatta tttaatatct cttggcttgt tgaataatct
     4141 acaaacacca tattttcact aaaatcagaa gataatcctg tatatgtttc ctgtttttta
     4201 ccagaaaaga tccctataat tttaaattta tgacttttta tttttccact tttttcaata
     4261 tctagtaatt caagatcaac ttcatcacct aattttagat tgttttgttt agcaaattct
     4321 tcatgaacaa taattgaatt cttatcattt tcttctatat ttttaccttc tttaattgta
     4381 aatactccac tactaaataa aatatttctt ttagtattat ttgtagcttc gagtgaaaca
     4441 acattcttaa attcgtcaga taaatcttct ctatttattc tttgttctcc gctaactact
     4501 ttagcatctt ttagttttgc taatccatca tattgaatta ttttttcttc aatttctttt
     4561 aatttttcaa tatctttaaa ttgattaaca ttaaaatatt taccatcttt ttttgttatt
     4621 gatattgaag aattagaact ttcatataaa gccttttcta tttcattgct tgatttcatt
     4681 attgttaaac aagaatacaa gcaagaaaga actatagtta atatgataaa aattattaaa
     4741 gttctatttt tctttcttgt aatatatgct atagcatttt ttatcacttt cctaactact
     4801 cctctctatt ttcttactcc ataactaatt gcatccactg gacaagcatt tttacatcgt
     4861 ccacatctga tacactccag atgattgcaa ttctcaactg aatctttatt catttggcaa
     4921 acttttttac attttccaca attaatacac ttatccttat c
//
LOCUS       DAZUKZ010000147         4808 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000147 DAZUKZ010000000
VERSION     DAZUKZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.153"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1118
                     /locus_tag="ACG8EF_002614"
     CDS             72..1118
                     /locus_tag="ACG8EF_002614"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4947870.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            1135..2358
                     /locus_tag="ACG8EF_002615"
     CDS             1135..2358
                     /locus_tag="ACG8EF_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4947871.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2447..3334
                     /locus_tag="ACG8EF_002616"
     CDS             2447..3334
                     /locus_tag="ACG8EF_002616"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4947872.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3420..3650
                     /locus_tag="ACG8EF_002617"
     CDS             3420..3650
                     /locus_tag="ACG8EF_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFS4947873.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(3707..4671)
                     /locus_tag="ACG8EF_002618"
                     /pseudo
     CDS             complement(3707..4671)
                     /locus_tag="ACG8EF_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
BASE COUNT         1628 a          795 c          975 g         1410 t
ORIGIN      
        1 aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat ataagtgaag
       61 ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt gcaaaatctg
      121 gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt atctcttata
      181 cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga atcaatagtc
      241 ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat gcgcattcta
      301 cagaggaaga tttccgaaat tcctttatcg gatcgaacca gctttctcct atcgtaaaaa
      361 aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca ccttattcaa
      421 aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc aatgggatcg
      481 acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa tatttcaaac
      541 tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt aaaggaatca
      601 ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg tttggtcatg
      661 ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat catcctgaaa
      721 atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat tcaggtgcag
      781 atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta gaagctttag
      841 caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg ttagaagata
      901 gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc caacagatta
      961 ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa gagcgaagta
     1021 tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat gaaaaagtct
     1081 ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa aaacatgaaa
     1141 atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac atcgatcaaa
     1201 acattaaaac aagaattaga aagacatggg catgaagtct atatttttac aacgaccgat
     1261 cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc atttatttct
     1321 ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt tgccaaagaa
     1381 ctagaactag atcttattca tacccataca gaattcggag cagggatact tgggaagatg
     1441 gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta tgaagactac
     1501 cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata cttgtctaga
     1561 ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat tgatacatta
     1621 cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga cattgaaaaa
     1681 ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc actaggtctt
     1741 caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa aaatatccaa
     1801 gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag attagttatc
     1861 gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact gcagttagaa
     1921 gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta ttataaagcg
     1981 gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta tacagaagcg
     2041 atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg attgttcgat
     2101 catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc acttattgat
     2161 tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact ttatgatatt
     2221 tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt tgtttatcat
     2281 gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa aataaaatta
     2341 aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt ttctagcgta
     2401 tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga aacttggttt
     2461 tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag caggacacga
     2521 agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag aaggagctat
     2581 ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta caatggtagg
     2641 ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt ctgcagatat
     2701 ctcaggacat attttagttg atttgactac aagtacacca agcctggcag aaaaaattgc
     2761 aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg gtggtgactt
     2821 aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg tttatgatca
     2881 agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt ctgctgggaa
     2941 aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga caggaatgac
     3001 ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaagtga ttgagacgct
     3061 ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta ttttaaaaga
     3121 agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga ttgcattaga
     3181 agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga aactatatga
     3241 agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca aactttggtg
     3301 gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta gagaaaatca
     3361 atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga ggacctccga
     3421 tgacacattc acaattagtc gcaatcataa aacgactgga ggctatggtc gagtcagcag
     3481 ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt acagtaactt
     3541 atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag caatacgaat
     3601 ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa attttaggat
     3661 aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaactaa tttagttgct
     3721 tttcagcttc ttttttgttt aacagttcaa tttcaaactg ttctttttct aattttttgc
     3781 ggtaactgat caagatttgt acaggatcga tttcttcttt tgacaattga cgtttcaaag
     3841 ataaatgagc ttctttcttt tcataataga agtcaggaga aacgtcacca gaggtaatca
     3901 ttgtttctgc aagttgacca taacaaacga ggtagtgctt tttctgactc tcatctgctg
     3961 ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa ctgcttttgt tcctggatcc
     4021 gtctaataaa ataagtaact aattcgatat ctttttgaag cataccggct ttttcgaaat
     4081 tcaactgata gatgaaatgt tgttgtcttt ttttcaagta agtcagtagc tttttatttc
     4141 ggccttgaaa cagcccttgg agttcctttt tttctgttca aaagtcattt cctgtaggtc
     4201 aggaagctgg actcgcaaag ctaacaaact aatttcagac aaccaaggca tttgataagt
     4261 ttcttccatt atacgcaaaa tagacggcat ttttttatag agacgaaaag gcccgtatgt
     4321 tcgagccgta ggagtatctg ttaatacaaa tcccttattg gtaatatgta cataattata
     4381 attactgaaa taattcattt gtcggttata aaaaggacgg taatgttgga tcaattgaca
     4441 ctcgagcaat aaagcatcta attccgtgtc tactacgacg aaatcaaaat ctgttatatg
     4501 atgaatcata cgtaatactt tttttgaatg ctgtttattc tgatgaaaat agctgcttac
     4561 tctatttttg agccgttttg ctttaccaac atagatgacc ttaccatctc ggtctttcat
     4621 aagatagaca cccggcagca tggggagagt tttcattttt tcttttaaca taggatctcc
     4681 ttttacgctt tgaataaaaa gactataaca aattttgaaa taatggacaa gttttaggga
     4741 attctatagt tctgttcaca gaaaatactt tgataatcaa aatatgtgag atagatgatt
     4801 tttctaat
//
LOCUS       DAZUKZ010000148         4788 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000148 DAZUKZ010000000
VERSION     DAZUKZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.154"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            564..989
                     /gene="bacA"
                     /locus_tag="ACG8EF_002619"
     CDS             564..989
                     /gene="bacA"
                     /locus_tag="ACG8EF_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFS4947874.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="ACG8EF_002620"
     CDS             1019..1183
                     /locus_tag="ACG8EF_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947875.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="ACG8EF_002621"
     CDS             2141..3118
                     /locus_tag="ACG8EF_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4947876.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="ACG8EF_002622"
     CDS             3128..3625
                     /locus_tag="ACG8EF_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS4947877.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="ACG8EF_002623"
     CDS             4001..4282
                     /locus_tag="ACG8EF_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947878.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="ACG8EF_002624"
     CDS             4284..4700
                     /locus_tag="ACG8EF_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947879.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1758 a          817 c          785 g         1428 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtacacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DAZUKZ010000149         4713 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000149 DAZUKZ010000000
VERSION     DAZUKZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.155"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            108..359
                     /locus_tag="ACG8EF_002625"
                     /pseudo
     CDS             108..359
                     /locus_tag="ACG8EF_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            452..1246
                     /locus_tag="ACG8EF_002626"
                     /note="aph(3')-IIIa"
     CDS             452..1246
                     /locus_tag="ACG8EF_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS4947880.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1656..1739
                     /locus_tag="ACG8EF_002627"
                     /pseudo
     CDS             1656..1739
                     /locus_tag="ACG8EF_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            1788..1871
                     /locus_tag="ACG8EF_002628"
     CDS             1788..1871
                     /locus_tag="ACG8EF_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFS4947881.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1996..2733
                     /gene="erm(B)"
                     /locus_tag="ACG8EF_002629"
     CDS             1996..2733
                     /gene="erm(B)"
                     /locus_tag="ACG8EF_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS4947882.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2996..3859)
                     /locus_tag="ACG8EF_002630"
     CDS             complement(2996..3859)
                     /locus_tag="ACG8EF_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS4947883.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3861..4133)
                     /locus_tag="ACG8EF_002631"
     CDS             complement(3861..4133)
                     /locus_tag="ACG8EF_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS4947884.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4150..4365)
                     /locus_tag="ACG8EF_002632"
     CDS             complement(4150..4365)
                     /locus_tag="ACG8EF_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFS4947885.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4457..>4713)
                     /locus_tag="ACG8EF_002633"
     CDS             complement(4457..>4713)
                     /locus_tag="ACG8EF_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HFS4947886.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1447 a          796 c          865 g         1605 t
ORIGIN      
        1 atttcttggg agttttgaga ctactatatg cgtttttttg ttgatttacc aacatgttta
       61 gacattttcc tctaggaaaa aattaactag caccacgggt tatcttttat atagaagata
      121 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
      181 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
      241 ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt
      301 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
      361 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
      421 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
      481 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
      541 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
      601 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
      661 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
      721 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
      781 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
      841 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
      901 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
      961 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     1021 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     1081 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     1141 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     1201 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga
     1261 tgtctaaaaa gctttaacta caagcttttt agacatctaa tcttttctga agtacatccg
     1321 caactgtcca tactctgatg ttttatatct tttctaaaag ttcgctagat aggggtcccg
     1381 agcgcctacg aggaatttgt atcgataaga aatagattta aaaatttcgc tgttattttg
     1441 tacatttaac ttgacggtga catctctcta ttgtgagtta ttagtggtac agttttcaac
     1501 cgttttaatt ataaaaaagt ggtgcatttt taaattggca caaacaggta acggttattg
     1561 caggtgtatt tcttatctat gggtttaaca tggattttat cattaaaatc atgagtattg
     1621 tccgagagtg attggtcttg cgtatggtta accctaaagt tatggaaata agacttagaa
     1681 gcaaacttaa gagtgtgttg atagtgcatt atcttaaaat tttgtataat aggaattgaa
     1741 gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg ttggtattcc
     1801 aaatgcgtta tgtagataaa acatctactg ttttgaaaca gactaaaaac agtgattacg
     1861 cagataaata aatacgttag attaattcct accagtgact aatcttatga ctttttaaac
     1921 agataactaa aattacaaac aaatcgttta acttctgtat ttgtttatag atgtaatcac
     1981 ttcaggagtg ataacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa
     2041 aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa
     2101 attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg
     2161 tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaatact
     2221 cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat
     2281 aaaattgttg ggaatattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt
     2341 gaaagccgtg cgtctgacat ctatctgatt gttgaagaag gattctacaa gcgtaccttg
     2401 gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt
     2461 aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt
     2521 acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca
     2581 aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg
     2641 aaacacgcca aagtaaacaa tttaagtacc gttacttatg agcaagtatt gtctattttt
     2701 aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt gtaaatttgg
     2761 aaagttacac gttactaaag ggaatgtaga taaattatta ggtatagggc acctctaata
     2821 actaccaatt aattcacctc aaaatgaaat tagaaaaaaa cagcgaaatt tttaaatcta
     2881 tttcttatcg atacaaattc ctcgtaggcg ctcgggaccc caacatgagg aaatgttttt
     2941 ataaaaaaag agtagttacc gttttttggt aactactctt ttcctttttt aaggtttaaa
     3001 tacctggaag tttaggtgtt ttgggtattg gtggtgtagg tggctgcaag ctttccaatt
     3061 tttgttgaat tgctttaaat tcaggtgtct cttggtgttt atttagaacc attttttgct
     3121 ctattcgttc taaagtcggt tgaatttctt tccctgatac tttacgattc aattcttttt
     3181 ctaaggtttc ttttggacta atagaaggtg tttccaaact tgaatagagt tttactcctt
     3241 ctctgttata tagccttata tcgctaaata agcccgtttt atgaagggtt tctaaattgg
     3301 tcggtaagtt tttgacaaca atatcatgcg cttgttttgg tgttgccctg gctgtcattg
     3361 gatcatctgc atacatggtt tcatatcgtt caattgttcc taaatatgag ttgattttag
     3421 gtactgccat gacatacatt tttgtttcat aacctttggc ttgaagcatt gttgcggttt
     3481 gaataggaac gtctgttgtt cgtcctgtac cttcgatcac caaattatac ccttgatcgc
     3541 tcaaacggct tatgatcgct tctgtcatgc gattagaata aggggtaacg tgttttacta
     3601 cgtctttttc ataaagtttc actagttcat caaaattagg gtgctgttgt ttaaaggtat
     3661 cattatcaat gacaataaca ttcccttgtg tttcttcaaa aattgctgat cgcaaactgg
     3721 ttttccctga ccctggttgc ccaccaagta aaaaagcggt tggcgattca accgcttttt
     3781 ttccttgaat caattcttct aaattatcat ttaagcgatt ctcaaattgt ttgtcagtaa
     3841 aattgactat atttgccata ttaagccact ttctctttat tcaaaacttg ttttgagtaa
     3901 cgattcattg accgccaata ctcatgaacg gcttgtaatt cttctaaaga ataatcaaaa
     3961 agatttacac gttttgcaag ctttaattgg tttaataaat tgacttccaa taattgagag
     4021 tcatttttat ttaattcatg gttcaaaaca tagtttaata ctcgattata aacgcttgcc
     4081 gataattcgt taatgatctc tatttcaaat gttttttcat acgtaactgc catttcattt
     4141 tcactccttt taaagtttgt caggtaaata tttccgtata tattcttcta aggcttgatc
     4201 cataacttct tttacgtttc cgccattctt tgctgtttcg atttttatga tatggtgcaa
     4261 gtcagcacga acacgaaccg tcttatctcc cattatatct ttttttgcac tgattggtgt
     4321 atcatttcgt tttgcttttt gtgcgcctaa atttcccaca atcactcact tctttctatt
     4381 tcttcttact cttattttat catcaacaat cacaaatcac aagtgatttg tgattaatca
     4441 cttgtgattt gtgattctat tctttttcgt tttctaattg aataattcgc tcaagcattt
     4501 caaaaaatac gttcttatac atggataaaa cttttttgtc atagccttta tgctctgtaa
     4561 tgccgttttt agaccaggta cttactttat tacttcgctt gataatattt tggaaaacca
     4621 agttatcttc tttatgttgc ttatacagtt cttccaggtt tgattttatc gttgcgctgt
     4681 ccgtatcaac taaataagga acaaaaccga tca
//
LOCUS       DAZUKZ010000150         4687 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000150 DAZUKZ010000000
VERSION     DAZUKZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.156"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..1574
                     /gene="gadC"
                     /locus_tag="ACG8EF_002634"
     CDS             63..1574
                     /gene="gadC"
                     /locus_tag="ACG8EF_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFS4947887.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            1587..2987
                     /locus_tag="ACG8EF_002635"
     CDS             1587..2987
                     /locus_tag="ACG8EF_002635"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFS4947888.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(3152..4592)
                     /locus_tag="ACG8EF_002636"
                     /pseudo
     CDS             complement(3152..4592)
                     /locus_tag="ACG8EF_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         1473 a          771 c          883 g         1560 t
ORIGIN      
        1 ataagcgatt ttccataacc ttggtaggaa tgacaaatta aataggaaag ggaagtaata
       61 atatgaataa aaaaaagtta tctctattcg gtttttttgc cctgacagct tccatggttt
      121 tgactgttta tgagtatccg acctttgcaa catcacaatt acatttggtt ttctttcttc
      181 ttttgggagg tattttatgg tttctaccag ttgctttatg tgctgcagaa atggcaacag
      241 ttgaaggatg gaaagatggt gggattttca gttgggttag tcaaacttta ggagaacgtt
      301 ttggttttgc agctattttt ttccaatggt ttcaaattac agtaggtttt attacaatga
      361 tctacttcat actaggagcg ctgtcgtatg taattaattt tccagagctt aatacagatc
      421 catttatcaa gtttattggt gtattagtca ttttctgggt actgactttt tcacagttgg
      481 gcgggaccaa tcgtaccgct aaaattgcta aagcaggatt tgtcattgga attgttattc
      541 cttcgatcat tctctttggt ttagcagcag cctactttat cggcggtaat ccgattcaaa
      601 tggctattaa tgcgaaagct tttattcctg acttttctca gctttcaact ttagtagtat
      661 ttgtatcttt tatattggct tatatgggcg tagaagcttc agcatcacat attaatgagc
      721 tggaaaatcc ccaaagaaac tacccattgg caatgttttt actagtgatt ttggccatcg
      781 ccttagatgc aatcggcggt ttctcagtag cagcggttat tccacaacat gacttgtctc
      841 tcagtgctgg tgtaatccaa acatttgaaa cgcttatttt acattttaac agtcatttac
      901 attggttggt aaaagtgatt gctctcatga ttgcctgtgg cgttatgggt gaagttagtt
      961 cttgggtagt tggtccatca cgagggatgt ttgcagctgc tcagcgaggt ctgttaccag
     1021 ttttcttccg gaaaactaat aaacaagacg ttccagttcc gttgattatg atacaaggaa
     1081 ttatcgttac tatttgggca gcagtcttaa cttttggtgg aggaggcaac aatttatcat
     1141 tcttagtagc aatctcctta actgttgtca tttatctcgt aggatattta ctatttttcc
     1201 taggttactt tgtattagtt tttaagaaaa aagacttaaa acgaacttac aatgtaccag
     1261 gaaaaactgt tggcaaaacg attatcggtg gaattggttt tattttatca gtgttcgcat
     1321 tgcttatctc ttttgtacca ccagcttcaa ttgcagctag tgagcaccga acataccaaa
     1381 tcatactact tattagcttt gtagtgactg ttcttttacc ttttatcatt tatgaagtac
     1441 atgataaaag tgctcacaca actattgagg aacctactca cgtgaaagct gaagacgtta
     1501 atcccgctgt ttatccagct gctagaggag agcaccacgt tacaaaaaat gatgaacata
     1561 ttttaaaaca ttaggaggaa ttcattatgt tatacggaaa agataatcaa gaagaaaaaa
     1621 actatttgga accaattttt ggctctgcaa gtgaggatgt tgacttgcct aaatataagt
     1681 taaacaaaga atccattgaa ccacgaattg cttatcaatt agtacaagac gagatgttgg
     1741 atgaaggaaa tgcgcgatta aacttagcta ctttttgtca aacgtatatg gaacctgaag
     1801 cagtgaaatt gatgacccaa acgttagaaa aaaatgcaat tgataaatca gaatacccac
     1861 gaacaacgga aattgaaaac cgctgtgtaa atatgattgc tgatttatgg catgctccaa
     1921 ataatgaaaa attcatggga acttcaacga tcggctcttc agaagcctgc atgctgggtg
     1981 gtatggccat gaaatttgct tggcgtaaac gtgctgaaaa attaggtctt gatattcaag
     2041 caaaaaaacc taacctggtg atctcttctg gttaccaagt ttgttgggaa aaattctgtg
     2101 tatattggga tgtggaactg agagaagtcc caatggatga aaaacatatg tcaattaatc
     2161 tagatactgt catggattat gtggatgagt acacaattgg tattgtaggt attatgggta
     2221 ttacttacac tggtcgttat gatgatatca agggtctgaa tgatttagtt gaagctcaca
     2281 ataaacaaac tgactataaa gtatacattc atgttgacgc tgcatcgggt ggcttttatg
     2341 caccatttac tgaacctgat ctagtttggg attttcaatt gaaaaatgtt atctcaatta
     2401 attcttcagg tcacaaatat ggtttggtat atccaggtgt gggttgggtc ttatggcgtg
     2461 accaacaata cttaccagaa gaattagtat ttaaagttag ttacttaggt ggagaaatgc
     2521 caactatggc tatcaacttc tctcatagtg cagcacaact aattgggcaa tactacaact
     2581 ttgtacgcta tggctttgat ggttatcgtg atattcacca aagaactcat gatgttgctg
     2641 tttatttagc caaagagatc gaaaaaactg gtatttttga aatcattaat gatggatcag
     2701 aattgccagt tgtgtgctat aagctgaaag aagatcccaa tcgcgaatgg acactatatg
     2761 atttatctga tcgtctgtta atgaagggat ggcaagtccc agcctaccca ttacctaaag
     2821 acttggatca attaattatt caacgcttag ttgttcgagc agactttgga atgaacatgg
     2881 ctggtgatta tgtacaagat atgaaccaag caattgaaga gttgaataaa gctcatattg
     2941 tttatcataa aaaacaggat gtgaaaacgt acggctttac tcactaaaat tcttcagaga
     3001 aatataaaag aagttaatta actaataaaa ggagttgtga ttaccacaca gctccttaaa
     3061 tctatttgaa taaatatatg atattcattt gttacactct caatcttagc tgttgttcta
     3121 ttcatcatta ctcttattgt aagtagttca gtcaaatttg cttgttcaat tgatcgataa
     3181 cagtatgaat tgtttgaaaa gtaatcggat atataattgt tttctcacta cctaagcctt
     3241 cttggtataa agaactatcg caaagtataa tccgctgagt acaaaagaaa gtgtcgtcta
     3301 ctatttctct aagttgttta caaatgatat tactaataga aaagtgttct cccaaaatgt
     3361 cttctaactg ttgtcgatag tataaatacc taaagtctga cggagctact atgaacactt
     3421 caattggaga tttccgttta tgctcattta aaagacttaa actaatggtt agtttacgaa
     3481 tagaattctc attccaacgc agaaataaat tgttttgttt tcgccattgt tcaagaattt
     3541 catgaacttc taagtacaaa gcgcgttgct cttcatttaa caagtaagtc ttatcaggtt
     3601 gaaatatctg aatatcagcc caaagtgagc ggataaacgg gagaagtgct ttatcaaaaa
     3661 gtatctgatt ttcttcagat atattcagag ataagataag tttttgactg agctcaatca
     3721 ccaaagggtt atctgctaca tggttttgat aaacaacgtt caagtctttt tgtaacaatt
     3781 caaagttatc gcatagataa tttctagagt tgaatagaaa gtagatataa aggcattcat
     3841 cttcgtcatg acataaagag aaagaaagtt gctctaatcc tttctgtatt agctgaaaca
     3901 agggagtatg tcgggcaact cttttttctt cctgactaaa atatattggg ttagtcttag
     3961 atctctgcaa accgatagcg atacctaata aaataaattg tttttcaact gtagggtaac
     4021 ttcttgaaaa aaaatgtttt tctacataat caatcaattg ttgaccgctt tgaataattg
     4081 atttatttag ctggatcaac tctgtccgat ttgtctcata gattatcgtt agtaacaaac
     4141 ttcgataatc cttttcagga atttcaaaat ggccatcagc atttaagtac tgcatttgtt
     4201 taaaaaaatt aatcaactca tttcgtaagt tgtaaacttt gcttaacgaa atgaaggtag
     4261 tatcagctat attactccaa ttgtgttccc ctgataaata atgtcctagt acattgaaaa
     4321 agtcagagcg ctgatagatg agttgagtga gtgccaacct cgaaaacgaa gagtcgaatg
     4381 cgactgtaaa gctacttcta gatttctcca ctgaaataat ttgctgatgg agtatgtcgg
     4441 taatgcattc caagtcgtta gtgacagaca gtgagtgggc aaacgttcaa ttgatctgct
     4501 atttcgcgaa tctgaatggt ccctttttcg tgtaattgct cacaaatgta aacttgacga
     4561 aaaatacttt tctccagata ggtttccaac atagtactcc tcctagtcat tttatacata
     4621 actattataa ctctaccaat cggaatagtc ctgtaatata ttgaatcaaa aatataaatt
     4681 ataaaat
//
LOCUS       DAZUKZ010000151         4646 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000151 DAZUKZ010000000
VERSION     DAZUKZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.157"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..717)
                     /locus_tag="ACG8EF_002637"
     CDS             complement(133..717)
                     /locus_tag="ACG8EF_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4947889.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(928..1131)
                     /locus_tag="ACG8EF_002638"
     CDS             complement(928..1131)
                     /locus_tag="ACG8EF_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947890.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1141..1563)
                     /locus_tag="ACG8EF_002639"
     CDS             complement(1141..1563)
                     /locus_tag="ACG8EF_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947891.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1801..2415
                     /locus_tag="ACG8EF_002640"
     CDS             1801..2415
                     /locus_tag="ACG8EF_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4947892.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2580..3404)
                     /locus_tag="ACG8EF_002641"
     CDS             complement(2580..3404)
                     /locus_tag="ACG8EF_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947893.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3428..3583)
                     /locus_tag="ACG8EF_002642"
     CDS             complement(3428..3583)
                     /locus_tag="ACG8EF_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947894.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            complement(4042..4299)
                     /locus_tag="ACG8EF_002643"
     CDS             complement(4042..4299)
                     /locus_tag="ACG8EF_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS4947895.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4292..4561)
                     /locus_tag="ACG8EF_002644"
     CDS             complement(4292..4561)
                     /locus_tag="ACG8EF_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4947896.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1533 a          816 c          626 g         1671 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
      841 aaagaaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
      901 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
      961 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     1021 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     1081 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     1141 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     1201 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     1261 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     1321 tatcgtttta gaagctgcta gagcaagtaa tggagaaact attaaataat tttcagtaac
     1381 agttaagtcg ttagttgggt taccaatggc tattccgtcc ctatctaaag gagttaattc
     1441 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     1501 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     1561 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
     1621 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     1681 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
     1741 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     1801 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     1861 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     1921 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     1981 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     2041 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     2101 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     2161 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     2221 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     2281 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     2341 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     2401 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     2461 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     2521 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     2581 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     2641 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     2701 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     2761 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     2821 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     2881 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     2941 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     3001 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     3061 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     3121 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     3181 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     3241 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     3301 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     3361 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     3421 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     3481 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     3541 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
     3601 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
     3661 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
     3721 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
     3781 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
     3841 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
     3901 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
     3961 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
     4021 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
     4081 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
     4141 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
     4201 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
     4261 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
     4321 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
     4381 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
     4441 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
     4501 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
     4561 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
     4621 aaatgattaa tattctaact tcttat
//
LOCUS       DAZUKZ010000152         4635 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000152 DAZUKZ010000000
VERSION     DAZUKZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.158"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..519
                     /locus_tag="ACG8EF_002645"
     CDS             175..519
                     /locus_tag="ACG8EF_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFS4947897.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="ACG8EF_002646"
     CDS             520..939
                     /locus_tag="ACG8EF_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFS4947898.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="ACG8EF_002647"
     CDS             complement(1031..1381)
                     /locus_tag="ACG8EF_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947899.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2677)
                     /locus_tag="ACG8EF_002648"
                     /pseudo
     CDS             complement(1347..2677)
                     /locus_tag="ACG8EF_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
     gene            2933..3499
                     /locus_tag="ACG8EF_002649"
     CDS             2933..3499
                     /locus_tag="ACG8EF_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4947900.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="ACG8EF_002650"
     tRNA            3644..3731
                     /locus_tag="ACG8EF_002650"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
     gene            4065..>4635
                     /locus_tag="ACG8EF_002651"
     CDS             4065..>4635
                     /locus_tag="ACG8EF_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFS4947901.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1685 a          778 c          891 g         1281 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatcaac gattagaggt tgtgaagaag ctgtcctgca tcaagtaata
     3781 agaacggcca aacaaaaata gcttttccta ttttttcgtt tgattgggct tattaccgca
     3841 gatgcaagct tttgaacacc gtttgttcgg aataaggggc tgtgacaaga cttttgtcac
     3901 agccccttat tgttgtactt ttaacctaca atattttttg attccccctg agtactattt
     3961 tttataaatg ctagtaaatc aatcttcttt ggcctttttt tgataaaaaa aatactagat
     4021 gtaagataaa agtgtaaaaa cgatttgttt taggagggga aacgatgtgt accgcaatca
     4081 cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg tcttataatg
     4141 aagtggtcac tattactcca agaaattatc gtttcgattt tagaaaggta aaaaatttag
     4201 ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca ctctattatg
     4261 aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc aatgctgatt
     4321 ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt ccatggatac
     4381 tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc aatctagtca
     4441 atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta gctgataaag
     4501 aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat aatcctgtcg
     4561 gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac aattatcgct
     4621 cgctatcaaa tggaa
//
LOCUS       DAZUKZ010000153         4609 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000153 DAZUKZ010000000
VERSION     DAZUKZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.159"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(83..397)
                     /locus_tag="ACG8EF_002652"
     CDS             complement(83..397)
                     /locus_tag="ACG8EF_002652"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4947902.1"
                     /translation="MLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQ
                     RYGATKIHQVLLKEGISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQ
                     KN"
     gene            complement(448..702)
                     /locus_tag="ACG8EF_002653"
     CDS             complement(448..702)
                     /locus_tag="ACG8EF_002653"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4947903.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(895..1170)
                     /gene="prgO"
                     /locus_tag="ACG8EF_002654"
     CDS             complement(895..1170)
                     /gene="prgO"
                     /locus_tag="ACG8EF_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFS4947904.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1142..2095)
                     /locus_tag="ACG8EF_002655"
     CDS             complement(1142..2095)
                     /locus_tag="ACG8EF_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4947905.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2707..4200
                     /locus_tag="ACG8EF_002656"
     CDS             2707..4200
                     /locus_tag="ACG8EF_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFS4947906.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4335..>4609
                     /locus_tag="ACG8EF_002657"
     CDS             4335..>4609
                     /locus_tag="ACG8EF_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFS4947907.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1493 a          774 c          801 g         1541 t
ORIGIN      
        1 cttgaattct tgtgaaaaag ttcttcgtgt tctcttagac ataaaaattc ctcctggtat
       61 gttttcttct agtctacaca ccttaatttt tctgtctagt taattgtagc ctatccatga
      121 tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt aaatttaatt
      181 gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt aataaaactt
      241 gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct
      301 ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta taaaaataat
      361 agacacttct agggatttct aataaccgac acaacaaaga aacagtgtaa tccttacacc
      421 atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg ctgcggcttt
      481 ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt cagctacttc
      541 ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt tgtaaatcgt
      601 tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag tttgattcag
      661 ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc atcctctcgt
      721 agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga tactaacacc
      781 aaatgttgta tacccccaaa caatacacat attttgaagg attaggatta ataaagctaa
      841 aaatatagta agtatactaa atatatttac tatatctagt atacttacta tattttaatc
      901 ttttgatcgt ctattatata taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc
      961 cattgcaagt ttgactattt catacatagg aatatctttt gcataactaa gatcccgaat
     1021 ggtttttagt acgggaggat ctacttgaac tggttttctt tctttcgcag tgtactttcg
     1081 ttcccctaac tcgacattaa tatcatcttt aattgaaata tcttcagcac caattttctt
     1141 cttatctttg ttcgttggta ataatgccat ttagtttaat ctcctttcca agaggtagga
     1201 tatgattggc cattgagtca gcatatttag gtgtataatc aaatccttca atatcaccat
     1261 gttgttcata gtattcaatt ctttcttcta attctgtaaa gatgtctgca aaaatagccc
     1321 accatcttcg atctacataa tcatttaatt ggataccatt ctcaccatac atttctaacc
     1381 gatccgagcc tttaacaatg cttttaaaaa tgtttgattc accatactta tcaattgttt
     1441 tttgatagtt tgcgtgttga gaggaacgtc gcactgagaa aagaacaggc aaaattccaa
     1501 tgatttgaag atgagactct tcaaaattcg tgacaatcgg aactaaaact ttattaataa
     1561 aatcttctgt tccttccatt gcgtaccgtt ttaattcttg cattgcaata atataatctg
     1621 cagcagttaa gaatgcacga accacattat ctgttgatgg tgggcagtcg ataaagatat
     1681 aatcgaaatt ttttctcaat tcttctaaag gtttaatgaa ggccaatgat ctcttacgac
     1741 gatcaggata tttttcggac aagtattcat taagttcttc agatccagga gagccagcca
     1801 taatatataa atgtggactt acttgagtaa tacctgaaac ccagtcccct tttttgattg
     1861 ctgcagacat ggatcgatca aagctagtaa tttgatacgt tgatccaatg ttggaagtag
     1921 tatttctttg ttggtcataa tcaattagca acacgtcttt ttcaaaaagt tgactagaaa
     1981 caatcgctaa taaatcattg tctgtagtct taccaatgcc gccttttcta cctgtaagca
     2041 ttacaacttt tcctagttta tctgatgttt taaatgcagt ccgtaagttt tccatagttc
     2101 cctccaattt cctatactaa atatatttag tatacttcat atacttaata tattcgatat
     2161 atctaatata ctccaaattg ataagaattc agactttata aggaatatag tggatatact
     2221 aaatatattt gctatattca gtatatcaga tatattcaaa atgtcaaaat atatttgtgc
     2281 gttttgcgtt gacaatttag agtgaaaaga ggtaaactta attctataga aaccacagat
     2341 aattcactat ttacaaagta gcaaaagcag tggtactctt taaacagaaa aattacataa
     2401 aaaaaagaga cggaaataat tgattttagc agaatcaatt atttctgaat cacatggtta
     2461 ggacatgtgt tcacgctcgt ctcaagaata tgtaagtagc taccaactac ttatattaaa
     2521 agttgatcgg ataccttttc cgattgcctt tattctacca aaaaaaggcg agcaactcaa
     2581 cagaagaacg tttatttagt gtacttaaat taaatattct taaacactga agagagaaca
     2641 catactatgg ctgagattaa agctaaactg tgttctcttt ttgtttgaga aaggacgtta
     2701 caatcgatgt tgagcgaaat ttattcagct attcttaaaa acggcttacg tacatacaaa
     2761 tatcgaaaca gcaccatgaa acctgcaggt catattgatt ccagtaggag tggtgctatc
     2821 tttgcgtacc gtagcaagca attaatgaat gttgggcgtg gtatggtcat tacttcggaa
     2881 gaagctattt tagagaacga aaagaaacta acgcattgga cacctaatac ctatcgcttt
     2941 ggtacttatg cggatgatta tcgaacagtg gttaaaggtc attctgagaa aaacttaagt
     3001 caaatcaaca catttgtagt tgatatcgat tcaaaagaga accatcaagg cgaaattatt
     3061 ttagcctgtt tagatcaagt gggctacatg ccaacgctaa ttttagaatc agatcatggt
     3121 tatcaagttt attttgtact aaagacacca ggctatgtca caaagaaaag caatttcaag
     3181 gtgatcgatg tagcaaaaaa aatttcaaaa acaattcgtc tacagttggc tgaaaaattg
     3241 tccggagtgg atctaggatg taaccacttc ggaattgctc gattcccaca agaaaataat
     3301 attgttttta aagaaatgaa ctatcagtat tcttttgctg actggattca atggtcgatg
     3361 aagcttgaat cagatcaaaa aacaaaagaa gatcgagaac gaaaactaat tgtctttcct
     3421 gagaaaaaag aaattcgtca agtagatgaa ccgtggttcg atatgttact tcataaagca
     3481 aacatcgtcg gcggagaagg acgtcttggt cgaaataatg tgattttcac gctttcttta
     3541 gcttattact cttcaggtta tgcattggat acttgcgaat acaatatgtt cacattcaac
     3601 gaacggttac aggaaccgct atctgaagca gaattagtaa agattgtacg tagtgcttat
     3661 tctggtaatt atcaagcagc aagcagagaa ttcattattg aattgtgcca gcaatgggtt
     3721 tcaccaacct taaaaaataa tgatctgttc attcaacgac gtggctggtg gaaatttaaa
     3781 aagccacgag atcaaagaaa atatagtcat aaaaatgaat ggaaagaaga tctattggct
     3841 tatctaagcg aaaaaagtct tgctagtgag ccttatttga acctgtcaaa aaaagaattg
     3901 gcttcacaac taacgatgcc caagcgaaca cttgataaga tcttaaatga gttacgagag
     3961 gagaataaag tcttctacaa ggtaacacga ggaagaaatg gcggaatgat cattgcttca
     4021 gtcaaaacgt tgttcgcgag ggttattcaa ctaagtaaag aaaagagact agattattca
     4081 acaaggatca cagaggtatt tggactcaac gagagcttag tagaagagac atttagacaa
     4141 ttatcaaccc ccttgaaccc tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga
     4201 aaaataaaag gtgcaaattg ccttatacaa tatatctatg atacttctgt aggtgttgct
     4261 ccctttgcct agctacgtgt aggtggtata cttataactg cgtatgcagt cttatgtatc
     4321 atggaggaag ttgtatgtcg ttaaaaaatt atgtctattc acatcctgtt aatgtgttta
     4381 tcattggtcg tttgggatta tctgtggaag tgttctgtga gctttatggt tttaagcagg
     4441 ggactttgtc ttcttgggtt accagggaaa aaacagttgc ttctttgccg attgagtttc
     4501 ttcatgggtt gtccttagct tctggggaga ctatggatca ggtatatgat tgtttgtgtg
     4561 tgttagagca ggagtatatt gagtataaaa tagctaacga gttaagaaa
//
LOCUS       DAZUKZ010000154         4499 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000154 DAZUKZ010000000
VERSION     DAZUKZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.160"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(48..659)
                     /locus_tag="ACG8EF_002658"
     CDS             complement(48..659)
                     /locus_tag="ACG8EF_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4947908.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(782..907)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(989..1705)
                     /locus_tag="ACG8EF_002659"
     CDS             complement(989..1705)
                     /locus_tag="ACG8EF_002659"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS4947909.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1711..2280)
                     /gene="scpB"
                     /locus_tag="ACG8EF_002660"
     CDS             complement(1711..2280)
                     /gene="scpB"
                     /locus_tag="ACG8EF_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFS4947910.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2264..3034)
                     /locus_tag="ACG8EF_002661"
     CDS             complement(2264..3034)
                     /locus_tag="ACG8EF_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFS4947911.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3031..3402)
                     /locus_tag="ACG8EF_002662"
     CDS             complement(3031..3402)
                     /locus_tag="ACG8EF_002662"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4947912.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3421..4308)
                     /gene="xerD"
                     /locus_tag="ACG8EF_002663"
     CDS             complement(3421..4308)
                     /gene="xerD"
                     /locus_tag="ACG8EF_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFS4947913.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         1222 a          981 c          786 g         1510 t
ORIGIN      
        1 ttttttccaa aaaaaaagac caagaaattt cttgatcttt ttgatattta tttgactgct
       61 gtatgacgtt gttcgaactg atgttgtttt cgacttaacc atggaagaag ttgcgcatgc
      121 agcactaaaa agacagcact cacgatgaat cctttgatca agttgaaagg aacgatccca
      181 atcagtacat agtttgctaa agacatgccc agcatctgtg ttgcgttcag ccctaaaaac
      241 attaagtaaa gaggcgtaat aacgaaataa tttgcgatac tcataaaaat agttagagcg
      301 atggttcctg taatgacacc taaaaatttg tttcttcctt tatgctgttt ctttacacta
      361 aagaaataat aaatcgggaa tgtaaagatc gttgttgata agaaactagc agtgtctcct
      421 accaaattat ccggagaaaa acctgtagtc agcagatgta atactgaacg caagaacgct
      481 gttgcaactc cggccgctgg accgaataag aacatgctga tcaataccga gatatcacta
      541 aaatcgatct tcaaaaaact aaaagccggc atgatcggaa aagcaaaata ctgtaaaact
      601 aatgcgatcg cagcaaacat cgctactgca accattttct gtacacgggt gttcttcatt
      661 tgaaatcctc ctgttaggac tcctcttcag cgaacttttt gacgtttctc tccaaatgaa
      721 aaaactctat tttccaggga aaatagagtt agagttattt gatcaagcgt cgtcaaataa
      781 gctctatctt ctttatccag actatactgt cggtatcgga atttcaccga ttcagctatt
      841 tttttgcaaa ataggtcgtg gactataacc accggtcggg aatttcaccc tgcccctgaa
      901 gacgaacctt atataatttt tgtttacata ttcattatac atatctattc aaaaaacgca
      961 acatctaaca attataaaaa gtaagtgttc attttgagtg gttcagcaaa ctgctgactt
     1021 cttttttgtt cagttcacgg taatccccag gacgtaggcc ttgtaaagtc agatctccgt
     1081 atttttcacg ttttagtttt tgcacaggaa gaccaactgc ttgaagcata tttttcactt
     1141 gatgattacg tccttcgtgg atcgtcaatt ccacgatact gtggtttgtc ttaggatcca
     1201 ctgataaaat ttggaattta gctggagatg tttttcgtcc ttcgatcttt attccttttg
     1261 tcaaaggtgc aagcatcgat tttgtagcga tcccttttac ctttgcaacg taaactttat
     1321 ccacttcatg ttttggatgt gtcaggcgct gagcaaaatc tccatcattt gtcagtagca
     1381 agatccccga agtatcgtaa tccagtcgac ctacaggata gatacgttct tttaccatcg
     1441 gcaaaagatc cgtaacaact tttcttcctt tgtcatctgc aacagcagaa ataactcctt
     1501 taggtttgta caataaatag taaccgtatt cttcttgata aattgggact tcatccactt
     1561 ccacagtgtc atgtttccca acttttatcc ctaattccct tacaacttgt ccgtttactt
     1621 ttacacgtcc tgcaaggatg aattcttctg cttttcgacg agaagtgatg ccagcgtggg
     1681 caattacttt ctgcaatctc tccattacaa ttattctcct ttattcattc catcaaagaa
     1741 tagatctggt gacacgtctt cttcaagtga aatttccatt tcttgtgtat caggcagttc
     1801 ttctaatgat ttcaaaccaa aataatctaa gaagtagcca gttgttccat ataatatcgg
     1861 ccgacctggt ccttctactc tacctttttc ttcaatcagt ttatgggcag ctagacgctg
     1921 taccgctcct gaaacctgaa ctcctcgaat ctgctcgatt tcagctcttg tgattggctg
     1981 tttataagcg atgattgcta aggtttcgac ggcggcttga gataatgtgt ttgatgtccc
     2041 ttgcgcatat cgcttaagta agggagccag ctctttttta gtcgttaata cataatggct
     2101 ttccgtttca aaaatatgca aagctgagtg cgagttttga gtgtaagatt cttttaattc
     2161 tttcactaaa cttgtgactt ctgcttcgct gagatttaac agtccgctta attcttcatt
     2221 actgatccct tcctcgccaa caataaaaag aagggcttca agttcactta atttcgtcat
     2281 gttcggtatc tcctactgct ggataaagca taattgattc atagttttct ttttgttgcg
     2341 caatgatctg cccttttttc atcaattcta gcaaagccat aaaagtagtg acaacttcac
     2401 ttttagaagg tgactcaaaa aaagaatcga aagtacagcc atgcgcattt ctttttatac
     2461 gctcttcgat aaataccatt cgttcttcga tcgttgtgtc atcgggcgtg atcgtttttt
     2521 ccactttttg agaaaatttc ttacgttcta agattttctg catggcacgg atcaaatcga
     2581 tttttttgac ttgtcctcgc ttcaacggct tttcagcatc cgctaaccca tcgatgttca
     2641 taggttcttt tgtgtaataa agacttcgtt cctctgcttt ttctgtcagt ctttcagctg
     2701 catatttata tttacggtat tctaaaagct gagtgaccaa ttcttctcga ggatcttctt
     2761 cccataattc ttcatcgtct gtgccgaatt cttggacagg aagcaatgtc ttgcttttga
     2821 ttgccatcaa tgtagcagcc atcactaagt attctcctgc aacagctagc tcgagtgttt
     2881 tcattgtatg gatataattc atatattgct ccgtgatcgt agcgatcggt atatcatata
     2941 tatcgatttc catttgctga atcagatgaa gcagcagatc gagaggaccc tcgaatacat
     3001 ctaatttaat attgatctcc gtcaactcat tcactctttc ttctttttct ttgctagcca
     3061 ttttccaatg taaggctctg tgatttcagt tgcacggatg gagacatccg ggtacttttc
     3121 ctgtaattca tctaacattt gaaagcccat attctcaccg cgataagacg gattcaaaga
     3181 aatgtcatgg atgatcagct gatcgttcgt tgcccagtaa ataccaataa tcccttgaat
     3241 gttggttgaa ccttctggcg taaagaaaaa aagttcataa tcgtcttcag attcgtacgt
     3301 atcaatctcc ttcaacaaag aagttttacc tgtcagtttc ttatggaaac tcaatagtcc
     3361 catagcaatt ttacggtttt ctttccggta atgtgttaac atcttctcac cttcctatga
     3421 ctaagccctt ggaaaatgct gcttataaac atccgtcatt cttttcttcg tgatatgtgt
     3481 ataaatctga gtcgtagaga tatccgcgtg acctaacaat tcctgtactg tccgcaaatc
     3541 agctccattt tctagcagat gtgtggcaaa gctatgccgc agcgtatgtg gtgttacttc
     3601 tttgtaaatc ccagcctctc gtactaactg cttcagattt ttccagattc cttgccgaga
     3661 aagacctttc ccatgattat tgacaaatac atgcatttca gatgcgtcct ttacaagtaa
     3721 tggacgtgct tcgtctaagt agcgttccag ccattggatc gcataatcac ctaaaggaat
     3781 gatccgctct ttatctcctt ttccaactgt ctgtaccagt ccaagagaaa gatgaagatc
     3841 tcccaatttc aatccaatca attcactgac gcgcatccca gtagcataca tgacttccaa
     3901 gattgcacga tctcttatcc ctaacgtttt tgtcgtatca ggtgcttcaa tcagcttttc
     3961 tacttctgtc agagtcaatg tacttggtaa tttctgtgct ttcttcggcg tatcgatatg
     4021 ttgcatcgga tcatgatcag tgatacgctc ttgccgtaaa aactgatgga atcttctgag
     4081 acttgagatc atccgagtaa cagttgctgt cgcttttttt tcttcatgca acttctccag
     4141 aaaacggatg actaaaaagc gatcgacatc ctgccatgaa gcaattttct gctcctgtag
     4201 aaaatgcaag tactgttcca aatcacgttc atagctttgt cgtgtattgg cggacaatcc
     4261 ccgttcaata ttcaagtagt ggagataatc gttgatttgc tctttcataa taatccccct
     4321 catgcctctc tatcatatca gataccgctt cattctgata gtctttttcc ataatctgag
     4381 caaagaaaaa gccgtctaga cactcagacg gactggtttc ttttttcggc tctataattt
     4441 atgggactga tgaatcagaa ttgattcttc agccccattt tcattttagg tattaaaaa
//
LOCUS       DAZUKZ010000155         4473 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000155 DAZUKZ010000000
VERSION     DAZUKZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.161"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            324..1052
                     /locus_tag="ACG8EF_002664"
     CDS             324..1052
                     /locus_tag="ACG8EF_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4947914.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1197..3224
                     /locus_tag="ACG8EF_002665"
     CDS             1197..3224
                     /locus_tag="ACG8EF_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFS4947915.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3332..4429
                     /locus_tag="ACG8EF_002666"
     CDS             3332..4429
                     /locus_tag="ACG8EF_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS4947916.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1572 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaatataat atacattttt tatttaatta taattaatat tgattgaagt ctaggcagtc
       61 aagtcacatt ttgatagata ggagggggac ttacgaatta ttttaaaggc tatgacagct
      121 gtatttctag attgtgtttg catcaagttg caataaaaac tagaaatcaa atcgggctga
      181 taatcattta taactaatcg atacatagga ccaaatatag gtctatgttc ttttatttca
      241 taagtatcgt aaaacagaat tttgctatgg attaatccaa tcgactgata tgtttagtaa
      301 ataaagaaag aggaatgtca atgatgaaaa aagaaatcgt attaacagca gcaatgatgt
      361 tattcagcat ggtagcaagt acgacttttg tttctgcaac tgaagtatac ccaaaggaat
      421 ataacacaga aggaacgatt acatttgaag caggagatga gggtgtaact cctcctgttg
      481 atccagagaa tcctgatccc aataaacctg tcgatccatc agatcctcca agccctggaa
      541 caggaggggc attatcgatt gattatggat caaaattcaa atttgggact caaaaaattt
      601 ctactgcgga taaaacgtat tatgcagctg ctgatgtgat gaacgatggg tctagaaaac
      661 cgacatatgt ccaagtgaca gataggagaa gtaccttatc tgggtggaaa ttaagtgtct
      721 ctcaaccaga acagtttaaa actgccagtg gggatgaact agttggagca cagctgaaat
      781 ttacgaaagg gcaggctgtt tctttggttg atccaacata tacgccgcaa acggtgaatt
      841 ctgaattaac gttgacaccg ggaggaaaca atacgttagc aattaatgct aaatcaggaa
      901 caggtgtggg gacttgggtc tatcgatttg gtgcaaatga aaatgaaaat caagatgcgg
      961 tacagctttc ggtacccggt aaatctgtca agttagcgca acaatattcc actaaattag
     1021 tttggacatt agaagataca cctaacaact agtttactga aaagaactgt taaaaataaa
     1081 tacgtagtca tattgtcttg cgaggtaatc gagcaaaaat tgtcaaaggc taggctaaat
     1141 ttgtttggat acgaatagtt catctaggaa tttaacgatc aggaggaaaa gatatattga
     1201 aaaagaggag gatcgtgctc attttaagca gtctcttagt gactaatatc tttccgatca
     1261 tagggtactc tttggaattg aatcagttgg agcatggaaa tagaagagac gaagaaagca
     1321 aactcacttc gacattcgac agtttaatag aatccaacca gacacaagaa agcagtgagg
     1381 atttaagtat ttcgagtagt ggagagacca ctgacaacgt agaagctgag ggttcggaca
     1441 aacaaggact agaagatgca ccaatttctg cggttgagct gcctgacgat cccaatatta
     1501 tttctatcga taaaattttt caaacaccta tcggtactag cacgtccata ttggaaaatg
     1561 ggaagttact gcagttgaac ccagcagaaa ggtctcaaag aggagctatt tggtcaaata
     1621 agaagattag tttactctcg gattttacat ttaaatctta tttatactta ggaaatcaac
     1681 gagatagagc gggagatggc atgaccttta cattgactgg cgatcctaga atggctagta
     1741 atcccagtca agttattgga tcgccaggta tgggaattgg ctcatattct actcgttctg
     1801 gccaacctta cattgcgaat gcgctctcga ttgagtttga tacgtataaa aatagtggtt
     1861 cttctgatcg aatggatcga gagataactg ctgataatag agggcatgga catgtcgctt
     1921 ttgtcacgcc taaaccaaat aataataatt attctggaga gcatactggc gtgacggtag
     1981 ctcctacatt tctttctaat ggcacatggc gcttgttaat aattaaatgg gacgcggagc
     2041 aaagaagtct tacttatgat ttggaaggtg tcggtcactc ttctcggatt atacctgatc
     2101 tcattaggca gtttggttct acagaagtaa attgggggtt tacttcttca acaggtactt
     2161 tttttcaaga aaatgctgtc gctgtcacgc gacttccatc gaatgtcaca tcaacagctg
     2221 atgtaaaagt caacgatagt gattatgggg ccatcgctga agctggagga aacgataaga
     2281 taactatcag aaatacattg aaggtcgagg gggattttct tgaatataag aaaatgcaga
     2341 ctagtatcga cctcccacaa gaattaacta ttcccgagca tatcttgatc aatggaaatc
     2401 aaattgatgc caaagatatc ttgataaccg gaaatcgagt cacgctaaat ctgacagatt
     2461 attttattgc tggagatacg ttaataatcg aattggagac aactttgcag gaagtaattc
     2521 ctgaaaaagt actcactatg cattttgaat accttgaaaa cgggaatttg attgagcaat
     2581 cgaatgaaat aaacctaaag atcgcaaagc tgaaggagat agatatccat gtttattata
     2641 aagataaaga cagtcagatt gatattgctg aaatgaaaac aatcactggg aaaataggag
     2701 agaagtataa agaaactcct attgatattg aagggtatgt ttttgactcg gattcagcga
     2761 atacagaagg catcttctcc gaaaatacag aggatatcta tttttattac agaaaaggca
     2821 aactttatct ctcaactgct cctcaatatg tcaattttgg aaaacataaa atatccagta
     2881 gaccattaag tgaattcgga cagttttcag gtgggttgaa aattatagat gaacgggctt
     2941 ttggaagttg gaaattacaa ttgaaacaag agaaagttct tacaaatgga gattttgagc
     3001 ttccagaagc tctttccttt gttacctctg aaaatacaac ggttataggg acatccgctg
     3061 ttacgatttt cgaaagtgat caaaagggtg aaagcgattt gtcctctctt ttagattcta
     3121 cagctcatcg tggaatcaga atagatattc ctgtagaaaa ccaaagaatc ggaacctttg
     3181 aggggaaact atcttggata cttgcagatg tcccaggaaa ttaacaggaa gagaagaata
     3241 taacaaaatt attgagtttc aagaagggca caggtaccat aggctcttgt gtcccacaga
     3301 gacaagattt tgttatagtc ttctcttaca tatgattaaa ggagcgattt tcttgagacc
     3361 aaaacaacaa ttatttataa cactcctagt tattttaggg gcacttttta acaatatatc
     3421 agtgtcagct agtgcattta atttttcagt gacgccgata acttcagaga atcaaatcga
     3481 caagagaaaa acttacttcg atcttcaact tgatccggat caggaagtag aagtaaaagc
     3541 ggaattgaga aatgatacag aaaaagaagt gaaaatcgat atttctgtga atagcgcaac
     3601 caccaattca aatgtgatgg ttgaatacgg aaaaaacgag attgaaaaag acatgagcct
     3661 gatctttgat ctggtagatt atgtgagcta tccacagata gtgacgctaa aacctaaaag
     3721 tgtccaaacc gttgcttttc atgtcaggat gccgaatgag cgatttgatg gtgtattggc
     3781 tggtgggatc acattccaag aaatccagac ggaaaaggac caaacagaaa ctaaggatca
     3841 aagcttatcg attgagaatg aatacaccta tattattgct ttgttgatgc aacagacact
     3901 gaacgaagtt gctccgaacc ttactcttca tgaagttaaa ccagatcaaa tcaatgcaag
     3961 aaatgtcatc ttagcaaacg ttcaaaatga tcaaaaaacc tatatcaatc aagtagttat
     4021 tgaaacaaaa attacaaaaa aaggacattc tgaagtttta tatcaagagg aaaaagaagg
     4081 attgcagatt gcgccgaata caaatttttc ttttccaact gctttaaatg gtcaaccgct
     4141 aaccccaggt gaatatcacc ttacaatgac tgttttagga aatgaaaatg aaaatggaaa
     4201 attttcgaga aaaaaaggaa atactacgat caactatacc aatcaatggg tatttgaaaa
     4261 agattttaca atagacggaa aagtagccaa ggaattgaac acgacggatg ttacgattaa
     4321 aaaagacaat aattggattt atatcttagt agcttcactt ttcttattgt ttattttatt
     4381 atgcatactt ttgtttgttt tgagaaaaaa agaagagaaa gaaaagtaag gttaagataa
     4441 caaatagtaa atttttatta ttgtaggatg gtt
//
LOCUS       DAZUKZ010000156         4425 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000156 DAZUKZ010000000
VERSION     DAZUKZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.162"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            690..1658
                     /locus_tag="ACG8EF_002667"
     CDS             690..1658
                     /locus_tag="ACG8EF_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4947917.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="ACG8EF_002668"
     CDS             1669..2484
                     /locus_tag="ACG8EF_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFS4947918.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ACG8EF_002669"
     CDS             2552..3262
                     /locus_tag="ACG8EF_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4947919.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQRKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ACG8EF_002670"
     CDS             3298..>4425
                     /locus_tag="ACG8EF_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS4947920.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1491 a          661 c          920 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aaaggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAZUKZ010000157         4379 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000157 DAZUKZ010000000
VERSION     DAZUKZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.163"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            390..1301
                     /locus_tag="ACG8EF_002671"
     CDS             390..1301
                     /locus_tag="ACG8EF_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HFS4947921.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1788..2054
                     /locus_tag="ACG8EF_002672"
     CDS             1788..2054
                     /locus_tag="ACG8EF_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947922.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2126..2467
                     /locus_tag="ACG8EF_002673"
     CDS             2126..2467
                     /locus_tag="ACG8EF_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947923.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2522..2941
                     /gene="ssb"
                     /locus_tag="ACG8EF_002674"
     CDS             2522..2941
                     /gene="ssb"
                     /locus_tag="ACG8EF_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4947924.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3018..3314
                     /locus_tag="ACG8EF_002675"
     CDS             3018..3314
                     /locus_tag="ACG8EF_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947925.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3329..3760
                     /locus_tag="ACG8EF_002676"
     CDS             3329..3760
                     /locus_tag="ACG8EF_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS4947926.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4065..4247
                     /locus_tag="ACG8EF_002677"
     CDS             4065..4247
                     /locus_tag="ACG8EF_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947927.1"
                     /translation="MQRTLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKR
                     FTEEIEQLAELNIRYF"
BASE COUNT         1608 a          675 c          934 g         1162 t
ORIGIN      
        1 aactaactcc cccctaaccc cctctttcag cctcttttga aagaggggga gaatcaaccc
       61 cacggccaac gccccagtgt tcttggggct gtccagaaca gaaacttgcg agagagtggg
      121 ggtaaaacaa gtgtagagga aacttgaaga cttgttcgaa cagaaacttg cgagagagcc
      181 ggagtaaaac aagtgtagag gaaacttgaa gacttgttcg aacagaaact tgcgagagag
      241 ttggagtaaa acaagtgtag aggaaacttg aagacttgtt cgaacacaaa cttacaagag
      301 aggggagtaa agcagttgca gaggaatcag gaagatttgt tcgaacagaa acttgcaaga
      361 aagcattatt aaattgaagg tgatgaaata tgaagtattt attgcatggc tattttattg
      421 attattctac tcgcaatagt agtactttag cgattctatg tttgcttcat gaattgagag
      481 tggttactag aaaacagcta tacaaactat tgaattttga cagatcaatt agtaaacaag
      541 gagtaacgaa tgctttagtg agattatatg aaaaacagtt aatcgatcga aaaaaatatg
      601 gaacagaaat gtattattat ttgacgaaag agggacatca aagcataggt gggtattata
      661 cgttgccgaa agttcctgaa tataatttga atcaccattt acaaattaac aattatttga
      721 ttaaaggaat ggcattggca aaagaccacc cacatttaaa acggattatt tcagaaagac
      781 gtaccgtcta tgaaacgaag gatttaaatc ctaagacaaa aggaagaaaa ttttttgtgc
      841 cagattttat ttttcgtttt cgagacaaag aaaatcatga gatcaattgg tcctttgaaa
      901 ttgagctaac gttgaaaaca cgtcgtcgct accgtgaagc gatttttccg aaatatattc
      961 gtgagttgcg aacacgtcct gaatcacatt taatttatgt gacacctagt ggcgcaattc
     1021 aagaagaact tcatgcgttt aagcgtattt ttttcaagcg cgaaactgaa aaagatgcga
     1081 gtcaacaaga attgtttgag cggttgcata ttttttcagc tttagagttt gaagagggat
     1141 taaaagcatt attagagaat gatccagcag tcaattggcg gacagtagaa cgagaaaaaa
     1201 tttatccgaa tggcgctgtt caaagtcgtt tgaatttatt tgaagaatca actgaaaatg
     1261 aggaaaaacc aaaagtgaag tttacgcctg aagtattgta aaagagggtg ttcgcttcgc
     1321 tctttttttg tgccagtttg ctggcaccgt tcagacgcaa gcgtctgaac cggctcgttt
     1381 ttaggaagga ctaacttgtt tgatttatcc gttgtcaaag aagtggacgt tcgagagtcc
     1441 ctaatcgtat agcagtcatt cggcgagtat aacctaaaac ataacctgcc aattgccaag
     1501 ggtaaaatgc acgctgcgcg cccttggcaa tcgtcagaac atgttttcga gccggttatg
     1561 ccgaacgacc gctatacgaa aagggcctct aaacagaagt ccactttgat ccttgacaac
     1621 tgaaacccaa aatcaaacaa gcaggccttc gtctaaaaac gagccgaatg aagaccttgg
     1681 gggttgcacc cccaaacccc cgtgcccccg acacactacc gaaaaggtgg gggtgggtgg
     1741 cgaaaaaatc aaataaaaca ttctattcaa gaaaggaaga aacaaccatg acaactaaaa
     1801 acgagcaact gaaagaacag gcaagaaaag agttgcaaga aagaggtttg attattgagg
     1861 ggagctttga aggagatttt gaaacctata ttggttgtta tgcaagaccg ataaacaaac
     1921 ctacagcctt agacccaatg aacgaacaag aagtaaaaga acaagaaaaa catgcagtga
     1981 atggttttcc gcaagatttc actgaatggt atgagtggga aattaacaac gggaagcttg
     2041 aaaacttctt ataaaagtca gatgacgtgc aaagagaatg attctctttg tacggtaaaa
     2101 atataaagaa agaaggaaaa agaaaatggc aaaaaacaca ccttatcaag taggagacat
     2161 tttttatagt agttggggct acgaacaaac gaacgtgact ttttggcaaa ttgtgaagtt
     2221 aacagaaaaa acagcatggt ttcagcctat tcaaaagaaa acggtcgagc attattcgtc
     2281 catgagtgga aaagttgttc caatgaaaga caagtttata gagtatcctt tagcaaaaac
     2341 taaagacagt attgtaaaaa aacgaattga tccagaaacc tatcctgata tgttatgtgg
     2401 ttatcgtagt tgggacacgt ttcatagata taaaaatcaa gctgtttatg aaagtagtta
     2461 ttattaaggg gatagtttcc cctttcaaaa ataataaata gaagaatgga gagaaaaaat
     2521 catgatgaat tcagtgaatt tagtaggacg gttaacaaaa gaagtggatt taaaatacac
     2581 accaaaaggc aatgcgacag gcacatttat tttagctgtt aatcgaaatt atacgaatgc
     2641 aaacggcgag cgtgaagcag attatatccg ctgtgtcatt tggcggaaag ctgcggaaac
     2701 tttagcaaaa tatactggaa aaggaagttt gattggcatt aatggacgtc ttcaaacgag
     2761 gagctatcag aatcagcaag gacagaccat gtatgttacg gaagttctag tgacagattt
     2821 ctatttgtta gagagtaaag aaatcaatga gcaacggtca aaatcaatgg cgaataatca
     2881 aaataattct tcaattccag gaaatgagat tcaaatttca gatagttatt tgccctttta
     2941 agtgaaaaat aattttgttc tatacctgat ttcttcaggt atagaacaaa aaataaatag
     3001 agatgaggcg attttttatg aaaatcattg acagttacga tcaacgaacc atgcaatatt
     3061 tgggctcgtt tgattataca gatgaaaata ttattaatta tgttgcaagt ttacctcctt
     3121 atcaaagcgt tcgcttggta gattttgcaa gtgatgaatt ggtattaaca acgatgggca
     3181 actttttaga ttatgtcgga gacaaaaaat ggttggaaaa aattttgtct aaattgattg
     3241 cgaaacaaac aggagaagaa cccataaatg cgattcaatt gttcaatcga tatgaacaag
     3301 aaaacaatga ttaaagagga gagaaaaaat gaaaagatta aaacgaagta tttttattgt
     3361 gctagcattg attgattttg ttgggacagg ttgtgaagca attgacgttc aaaaagctcc
     3421 cttatcggaa ataaaacaag caataacaaa acaagatgaa gaacaaccgc taaatacaca
     3481 agtagaaata gtgaatcaaa ataaaccgag tttttctgaa gaagattttt cgttagaaaa
     3541 tggaaccagg caattttttt cagatttgga tcgtttaaat cgggtaggtg tagcaagtgc
     3601 acttttgcat aaaagcagga tgccaacaga agaacgagac cgattataca ttaagccaac
     3661 tggctggaaa caaaagaaaa tgaagaatgg cgattatctt tataatcgtt gtcatttgat
     3721 tggctatcaa cgtgcgacaa gtttccccat aagtatgtaa tacgaaacgg gaggattaat
     3781 cttaagatta atcacaacta acctatttga cagttggaat gaaagctatc atgtctgctg
     3841 aatcaactgg tctgatactc ctacatgcaa taaagtctac taggaagtta ttgtatgaag
     3901 ctaggtaaag gcggcagaac aatttctaag tgtggaatca tatgaagttg gatatgccgg
     3961 gacgctataa attactcagg gtgagactgt ttttaattaa actgacgaac ttgcgaatgt
     4021 acgggtctat aaccgactta tattcgaaag atataagatt gcgaatgcaa agaacattat
     4081 cgaaaagtaa gaatgttggt catgaaattc gagatggaag actagcggtg tatctgtcaa
     4141 gagttggtaa aacatgtttg attgattcag atgagagtgg gaatgcgaca aaacgtttta
     4201 cagaagaaat agagcaacta gcagaactga atatacgata tttttaaata ctttagcgga
     4261 gaataaaaaa atcctctaca gaaaactacg gagggcaaca tgtgctattg tttcaacaat
     4321 acatttttaa tttttaccat aacaaaaaag tacttgaaaa aaaacagaaa tatgttaaa
//
LOCUS       DAZUKZ010000158         4348 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000158 DAZUKZ010000000
VERSION     DAZUKZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.164"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(387..1853)
                     /locus_tag="ACG8EF_002678"
     CDS             complement(387..1853)
                     /locus_tag="ACG8EF_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4947928.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /locus_tag="ACG8EF_002679"
     CDS             complement(2144..4228)
                     /locus_tag="ACG8EF_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4947929.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1415 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaattt
//
LOCUS       DAZUKZ010000159         4282 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000159 DAZUKZ010000000
VERSION     DAZUKZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.165"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACG8EF_002680"
     CDS             complement(37..1257)
                     /locus_tag="ACG8EF_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS4947930.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACG8EF_002681"
     CDS             complement(1277..1888)
                     /locus_tag="ACG8EF_002681"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS4947931.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACG8EF_002682"
     CDS             complement(1931..2863)
                     /locus_tag="ACG8EF_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFS4947932.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ACG8EF_002683"
     CDS             complement(3284..4144)
                     /locus_tag="ACG8EF_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4947933.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAZUKZ010000160         4220 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000160 DAZUKZ010000000
VERSION     DAZUKZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.166"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..351)
                     /locus_tag="ACG8EF_002684"
     CDS             complement(13..351)
                     /locus_tag="ACG8EF_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFS4947934.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(364..549)
                     /locus_tag="ACG8EF_002685"
     CDS             complement(364..549)
                     /locus_tag="ACG8EF_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4947935.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALVGISGAYLSDILNGNRDG
                     KKAQEHIETVKKILGIQ"
     gene            852..1226
                     /locus_tag="ACG8EF_002686"
     CDS             852..1226
                     /locus_tag="ACG8EF_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4947936.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            1231..1659
                     /locus_tag="ACG8EF_002687"
     CDS             1231..1659
                     /locus_tag="ACG8EF_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS4947937.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            1709..2362
                     /locus_tag="ACG8EF_002688"
     CDS             1709..2362
                     /locus_tag="ACG8EF_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947938.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETNISKTKET
                     TEISSSTSKSTSKTDSSSTVTSSNQATAKPSPTVISSSQDTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNTEAQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            2499..3149
                     /locus_tag="ACG8EF_002689"
     CDS             2499..3149
                     /locus_tag="ACG8EF_002689"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFS4947939.1"
                     /translation="MKKNLILGFICTSILTLGACSSPSNNATNSETQKTINSLKKENE
                     ELKKQIASQSNDTEENSESIDNASKEKTLPVVGILEYTISSIKTEQVTNDEDNFTNAE
                     YNFSGIENFPEKYYRTTISYKLKNKATSPFSLSSYQASLIDGDGMEYSRTAGEYFLYD
                     ENSNSLVQPETTTSGVFYLLSKNKPNLSTFKLNASKQFTDDFTAEPIGEAGTTEYK"
     gene            3259..>4220
                     /locus_tag="ACG8EF_002690"
     CDS             3259..>4220
                     /locus_tag="ACG8EF_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4947940.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
BASE COUNT         1576 a          704 c          698 g         1242 t
ORIGIN      
        1 actagcgctc tattaaattt tcccatccgc ttgcctcctt aagatttccg aaaaatttgt
       61 ttctaaaaat tcaagtgttt tacttcttaa aaatagatat gtgtctcttc cttcaactgg
      121 ataatagaca aatccatttt tgtttttctc gatatcgata atatttctat atcttgggtt
      181 ctttaaaact ctagaagtaa accaatcata ttttctgtta atccgttcta gcacttctgg
      241 caacgtcatc catctaccag tatcatcagc tttttttaac tcctcgtaat ccacttgaga
      301 gataattaca tagccttctg gaactgggat tttcgcttct agatattgca ttagctgttc
      361 ctcctattga atacctaata ttttttttac agtttctatg tgttcttgag cttttttacc
      421 atcacgattg ccgtttagga tatctgataa ataagctcct gaaataccaa caagcgcagc
      481 tagttctttg aaagtcattc ttctttttct catctccgct cgaattttta agtctaaatt
      541 ctcagacata aaaatagctc ctttctaaaa aataatttgt aagctaaaaa attagctaaa
      601 tcgttgacac ctattagctt ttaagctatt atgaatacat agttaaataa gacttataaa
      661 aagcctctaa aataacattt ctaagtttgg cgaccgagag aatgttttaa attagtagag
      721 ttttttgttg ctcttattta gctaacaatt tagcttacga attaaatata ctagcttaaa
      781 agctaattgt caaccaaaaa tataactttt aagctattta ttttcttttc agctttgaaa
      841 ggttgataat aatgagttta gttactaaga ttaaagaatt agcagacgaa aagcatgtga
      901 ctatagcaga agtagaaaga caggtgggca tctctaatgg acaaataaga agatgggata
      961 aagcctcgcc aaaatctgag aacttaaaga aagttgctga ttattttggt gtcacaactg
     1021 attatttatt gggaaataat aatgttccca aatgggctac aaaagaggaa gtggttgaac
     1081 ttgataaact actagactca aatgttaata tgtcttatgg tggggaaaca ttgacacccg
     1141 aacaaataca gcgcgtaaaa gatatcctga tagcgacttt ctgggatatt gtgaaagaag
     1201 acaaagaaaa aggcaaaaag atgtgagctt atggagatgg atacgattaa tttagtcgag
     1261 gagttgaagc ggaaatacca gtccgctaat cctttttata tttgtgaaaa gatgggcatt
     1321 aaaattcaat acgttccttt tatcgagaat cccaaggggc agtttcaaga aattagagat
     1381 cgtgcgataa tctttttaaa tgatgaactg cgagactctg aggaaagatt ctacatttgc
     1441 gctcacgaat taggtcacgc tatttttcat cgtggcttat ccagctatta tgtatcaaca
     1501 agaacatcta gaagcaaatc tgaaagcgaa gctaattgct ttgctgctaa tctcattgtt
     1561 tctctttata aagaagataa tgatcaatat cctaaaaaaa tcgaggaatt aaagaatctt
     1621 tacggtcttc cagaaagcgc ttatcgtttt cttatataaa aaagcccgtg ttagcacaca
     1681 tattacaacg agaaagagga attataaaat gaaaaaagtt agcgttatgt tgttgttaag
     1741 tactgctctg ctactttcag cttgttcaaa taataaaaaa gctgaatcaa cagatgccac
     1801 ttctaaccaa gaaacaaata taagtaaaac aaaagaaaca actgaaatca gttcatctac
     1861 tagcaaatct acatctaaaa cagattctag ttcaacagtt acaagctcca accaagctac
     1921 ggcgaaacct agcccaacag ttataagctc cagtcaggat acaatccaaa ccgcacctca
     1981 agaagaaaca tatgaacaga tgaaacaacg cactttacag tcaactccag ctgatcgtgc
     2041 aaattggtcc aacaaagagt gggaagcttt cggcgtggcc ctttatgaaa atggattgac
     2101 tacagatgat gctggcaata ttatcagtca agatcagaaa gaacaacaag ctgcatctca
     2161 acaaaataca gaagcccaac aaacaagcgc tcaacaagac gctgacactt tatcacttac
     2221 tgattttgtt aacaaatacg ggatgtcgcc tgttgcatgg aaagtacaga atggaatgtc
     2281 tgaagaagaa gcattgcgta caacacagca aaaaacttcc ggtgaagttc aattaggatt
     2341 ttctaaatac ggaatacaat aatatatttt tgtgtcccac tatcttgcct ataacctcta
     2401 aaaagttata aagaaaaaag cccgtgctgc aacacggact aaaatctcat ttctaagatt
     2461 ctacataaaa ataatatcat agaagtgagg aattaaaaat gaaaaaaaac ttaatattag
     2521 gatttatttg tactagtatt ttaactttgg gtgcatgtag ttcgccttca aataatgcaa
     2581 ctaatagtga aactcaaaaa acaatcaata gtttaaaaaa agaaaatgaa gagcttaaaa
     2641 aacaaatagc atcgcagtca aatgacactg aagaaaatag cgaatctatc gacaatgctt
     2701 ctaaggaaaa aaccttgcca gttgttggta tcctagaata tacaatttct tctataaaaa
     2761 ctgaacaggt aacaaatgac gaagataatt ttacaaatgc cgaatataac tttagtggaa
     2821 tagaaaattt tccagaaaaa tactatcgta ctactatttc ttacaagtta aaaaataaag
     2881 caacatcacc tttctctttg tcttcgtatc aggcatcttt aatagatgga gacggtatgg
     2941 aatatagtag aacagctgga gaatatttct tatatgacga aaattctaat tctttagttc
     3001 aacctgaaac gacaacatct ggggttttct atttactttc taaaaataaa ccaaatttaa
     3061 gtacatttaa acttaatgcg tctaaacaat ttactgatga ttttacagct gagccgattg
     3121 gcgaagcagg aacaacagag tataaataaa aaacacgccc cctccctcgc caaagtttgt
     3181 ggacgcgtta aaaacaaacc tacacaatag gcttctttac agtgcctatt gtatcagaga
     3241 aagagagtag attcaattat ggcaagatta gtcaaacgtg gaaatagttg gcaatacgaa
     3301 atttcataca aaaaagatga cggaaaatac acgaagataa gaaaatcagg attcaagaca
     3361 aaaggcgaag caaaagatgc cgccaacgaa ttagaatata acctgaataa aggtcttaaa
     3421 ggggatcgca aaaatctatt attatcagat tactttgaag attggatgca actctacaaa
     3481 gaaggaacag tatctcctat cacttataga aaatacgaag atacgttaat gaatataaag
     3541 aaatatatgc cagcagtatt gatttctgat ttagatagag ttggatatca acgcttttta
     3601 aataagtatg cgaaagacca tgtaaaatcc accgttatta agtttaataa ccatattaga
     3661 gcatcgttga aagatgccgt agaagaagga ttaattccgt ttgatccaac tagaaaagca
     3721 gtaatcaaag gaaaagattc attgaagcca aaagaagata aatatttaga ttatgatcaa
     3781 tttaaatctt taatgaaact cgtagaagaa aacctttctg cacagtactc ttctcctatg
     3841 ctcgtgttag ttgctggtgc tactggaatg cgatttgctg aacttctagg attaacatgg
     3901 gaagatatcg atttcgaaga tcaaatcatc actattaata aaacatggaa ttataaatta
     3961 aatgaatggg gaaaaacaaa aaacgagact tcaaatcgga aaatttcaat tgataaacat
     4021 acgattgatc tcttaaaaaa gtttaaaatc aatcaaaaag aattattcga gaattttgaa
     4081 gttaaaaacc ctcataattt tgtttttttc aacttaaaaa atggattagt ttcatcaaat
     4141 gccgtcagca aatatttgcg caaaaaatta aaagaattag ggattgaaaa gcaatttact
     4201 ttgcatggac taaggcatac
//
LOCUS       DAZUKZ010000161         4177 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000161 DAZUKZ010000000
VERSION     DAZUKZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.167"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(115..1620)
                     /locus_tag="ACG8EF_002691"
     CDS             complement(115..1620)
                     /locus_tag="ACG8EF_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4947941.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1617..2303)
                     /locus_tag="ACG8EF_002692"
     CDS             complement(1617..2303)
                     /locus_tag="ACG8EF_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4947942.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2496..3917)
                     /gene="gndA"
                     /locus_tag="ACG8EF_002693"
     CDS             complement(2496..3917)
                     /gene="gndA"
                     /locus_tag="ACG8EF_002693"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFS4947943.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1163 a          876 c          714 g         1424 t
ORIGIN      
        1 tctatttaat tgttcgctag tttttttata atgacatccc tgaatttctc acttgcttgt
       61 attttgtacc ttattctttg ttggatatat aaaaaaacaa gtaaagcaca tgctttactt
      121 gtcttctgcc tttgcgatag gaataaagat tctaaaaatt gtcccttgcc ctactacact
      181 ttctgctaag atacgtcctt tatagttttc aaccagttgt ttagcgatag acaaccccag
      241 accatttcca cccttcgttc tagctcttgc tttatccact cgataaaaac gattgaagat
      301 cttattaagg tcttctttgg gtattccttc gccaaagtcc tgaatcgcga tctcaaattc
      361 attcagagtg gaagaaatag agatatggac ttcttttcgt gtagttgaat actttactgc
      421 gttatccaat atgataatga ttaattgttc aaagtgatta cgataaattt gcagggtcac
      481 ttcttttgtc aaatcatcat ctaaagtgat cacatagtct ggatataata tcttgaaatt
      541 attataaact tgataggtga cttcttttcc tttagttgtt tcattcgcat aatgaacatc
      601 tacttgttct gctcttgata agtctagcat ctcttgaact aagcttttca tacggctgat
      661 ttcttgcatg ctggcgctta atgattcttc taatatctca ggatcatctt tcccccaacg
      721 atttagcaga ctaagatgcc cttcaataat tgcaacaggt gtccttaact catgagatac
      781 gtcttccacg aattgctctt gttgttcaat atacaaacgc atacggtcaa gcatctcatt
      841 gaatatttca gctaaatctg ccaattcatc atttgtgtcg atttcaggca tgtggatatc
      901 tgattgcgga tctttacgta tagtatccat tgtatctctg agtaccttga gtggttttaa
      961 gaaataagaa gataaaaaga aacctaaaat actacttaag atcaaagaaa caacttctaa
     1021 aacaataaga gtcaataata gcttatttcg aatatcatag aaagaagata attcatagaa
     1081 gctttgcgca tagccgatct tttctcttgt acctttagaa tatataggct ggatagataa
     1141 aaagccagtt tttccttcta gtgtaacgat cgttggaact ttacgtgtag tctgaatcag
     1201 gtttcgactg ttttcatggg ttttaaaaat caagttctcg tccaaatcat aaacataaag
     1261 atccattgat ggctgcccta gctcagctat aaaggaatcg atcttcatta agtttccttc
     1321 gaccagtgaa gtatgatcta cgacctcatc tccgtcattt gtcgctttcg tcagattacg
     1381 atacgtattt actaatgtca gttcttcatc cgagttagcc aaacgagaag ctacttctga
     1441 aatcgtacgc tctacattgt ttctttcttt tgccacaatc agattgatag atgatttata
     1501 ggtaatgaca gcgaaaattg taaacacaac gaatatgaag aaagaactcg caaaggccca
     1561 cttgattgtc aaggagggcc ctttcagttc tcttttttcc gattttttcg gctttatcac
     1621 gagcgcatca cataacctgt tccgcgtaca gtttggatat agctttcctc cccgggaacg
     1681 tcaattttgt tgcgaagata acgaatatat acgtccacta cgtttgtttc aacttctgtt
     1741 tcgtatcccc acactttatt tagtaaaacg tcacgtgcta ataccacgtt cacgttttcc
     1801 attaaagtaa gcagcaactc gtattctctt tttgttagtt caattacttc atttccgcga
     1861 cgtacgacac ggttttcttt ttcgatagtc aaatcgcgat aagtaatagt agtttgttta
     1921 gccacatttt tatcgccttc aatatcgatt cgacgcaaca atgcacgaag acgtgctaat
     1981 aattcttcga tagcaaatgg tttgacgatg tagtcatctg ctccgtggtc aagtccagat
     2041 acacgatcga tcacagaatc tcttgcagtc atcataatga ttggtgtatt tttgacttgg
     2101 cgtacacgtc tgcagacttc taaaccgttc aattctggga gcatcagatc caacaatatg
     2161 gcatcccact cattatttaa tgccgcatct aaaccagttc ggccgttgta gtggacttct
     2221 gcgttatacc cttcatgctt caactctagc tcgacaaatc gtgccaagtt cttttcatct
     2281 tcaatgatta gaatattgct catttattat tttatccttt ctattctact ggctttttaa
     2341 aaactctatc attataccga taatatgctt aaaaatctat agtcatttta tcatggatag
     2401 caattttaaa aaagagctga agtctactca taacaatcga cttcagctct ttcgtcctct
     2461 ttttattttc tcatttccta acagccgaaa gactattatt cttcatggta ccatgagtaa
     2521 tggaagattc cttctttgtc gacacgctca taggtatgtg cgccaaagta atcgcgttgg
     2581 gcttggatta tatttgcagg cagacgttct gctctgtatg aatcgaagta agcgatggct
     2641 gatgagaaag tcggtacagg tacgccagct tggacagcca aggcaacgac gtctctgact
     2701 gattgctgat attttttcgt gatttcaacg aagtaatcat ctagcaataa attttcgatc
     2761 tcaggatttt tgtcataggc atccgtaatt ttttgcaaga atcgagctcg gatgatacat
     2821 ccagcgcgcc agatttttgc gatttcacca aatggcaagt cccaatcaaa ttcttttgaa
     2881 gcgacacgta attgagcaaa tccttgggca taactcatga ttttactgaa atacaacgct
     2941 tctcggattt tttcgaccaa ttcttttttg tctcccgtga actcaaaatc gttcgtacga
     3001 gacaaaatct tgcttgcttg tacgcgttct tctttataag cagaaatata gcgggcaaat
     3061 acagattcag tgatcaatgg aagtggcaca cctaagtcaa gcgcgctttg gcttgtccat
     3121 ttacctgttc ctttattccc agctgcatca aggatcacat ctactactgg tttacctgtt
     3181 ccttcatcgt ctttacgtgt cagaatgtca gcagtgattt ctatcaaata gctgtctaat
     3241 tcgccttgat tccattcttt aaagatatca gccatttctt ctactgaaag gtttaaaata
     3301 ttcttcatta agtcataaga ttcagcaatc agttgcatat ctccatactc aataccgttg
     3361 tgtaccattt tcacataatg tccggctcca tttgggccaa tatatgtgac acatggtgct
     3421 ccgtcttctg cttttgcaga aatttgctct aagattggtg caactagcgc ataggcttct
     3481 ttttgtccac caggcatgat tgaaggtcct tttaaagctc cttcttcacc accggaaacg
     3541 cctgtaccga taaagttgat gccagagttg gctaactctt cattacgacg catcgtatct
     3601 ttaaagaatg tatttcctcc gtcaatcaaa atatcgcctt tatcaaggtg aggaagtaac
     3661 tcttggattg ttgcatctgt tgccggtcct gctttgacca ttagcaagat ccgacgtggt
     3721 ttctcgatag attctacaaa ttcttcgatt gtatatgttg ctttgaaatt tttatctggg
     3781 tgttcttcta ctacagcggt tgttttagcc cctgtacgat tgaataaagc cactgaatag
     3841 ccgcggcttt ctatgttcaa ggctagattt ttacccataa cggccatacc gacaacgccg
     3901 atttgttgtt ttgacatatg aactcctcct acatacggga tttctcccta atctcttaca
     3961 ttatagctta acacggctac attgcaaact aaagaacaac atattttggc tctataattt
     4021 atgggactga tgaatcagaa ttgattcttc agccccattt ttattttagg tattaaaaaa
     4081 catatcgttc tctagtatga ttaaatcacg acaaaataaa caactgagag gacgatatgc
     4141 tatatgaatg attctatcaa aaaaatgctg cgattaa
//
LOCUS       DAZUKZ010000162         4170 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000162 DAZUKZ010000000
VERSION     DAZUKZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.168"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..985
                     /locus_tag="ACG8EF_002694"
     CDS             <1..985
                     /locus_tag="ACG8EF_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFS4947944.1"
                     /translation="NHFKYLILSFISTFLNIAISVALMQTLYFDDKYVGRVVGGTLSF
                     VLIGLVIYLRIMIKGAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGP
                     AAGGIYSYISNIGIILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLIT
                     MIVMVVSIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIG
                     TVASAIVNILLNMIFIPRLGIIAAGYTTVFTYFLLFIFHYNIAKKITNKQLFDTKKIC
                     IIIIFVSIFSLILHILINQMLIRYVLTSLLIISLVIYFLKIKNRIIDDYNS"
     gene            998..2005
                     /locus_tag="ACG8EF_002695"
     CDS             998..2005
                     /locus_tag="ACG8EF_002695"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFS4947945.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            2017..3021
                     /gene="neuB"
                     /locus_tag="ACG8EF_002696"
     CDS             2017..3021
                     /gene="neuB"
                     /locus_tag="ACG8EF_002696"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFS4947946.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            3018..4166
                     /gene="neuC"
                     /locus_tag="ACG8EF_002697"
     CDS             3018..4166
                     /gene="neuC"
                     /locus_tag="ACG8EF_002697"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFS4947947.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
BASE COUNT         1532 a          526 c          764 g         1348 t
ORIGIN      
        1 taaccatttt aaatatttga ttttatcttt tatatctact tttttgaata ttgcaatctc
       61 tgtagctttg atgcagacat tatattttga tgataaatat gttggcagag tagtcggcgg
      121 aacactgtca tttgtgttga taggtttagt aatttattta agaataatga taaagggtgc
      181 tcattttttt gataaaaaac agtggagata cgctttaaaa atctctttgc cgctaatccc
      241 acattcctta gcaaatatag ttttagcaca attcgataga attatgatta attcttattt
      301 aggtcctgct gcggggggaa tatattccta cattagtaac ataggtatta ttctaagcgt
      361 aatatggatg tctactaata acgcctgggt tccatggttc tatggtgaga tgagtaaaaa
      421 taatgaaaaa aaagtgaaga aagttagtaa ctattattta gtagtcttta ctttgattac
      481 gatgattgta atggtagttt cgattgattt agcaaaattt atggctccga aagactatta
      541 ttctgggtta tctttagtgg ttccgataac cttaggctat ttctttcagt ttttatatag
      601 tttaccagtt aatgctgaat tttatgagaa aaaaacatcg tttattgcga ttggtacagt
      661 agcaagtgct atagttaata tactattaaa catgattttc atacctaggt taggaataat
      721 tgctgctgga tacacaaccg tatttacgta ttttttactt tttatttttc attataatat
      781 cgctaaaaaa attacgaata aacaattatt tgacacaaaa aaaatatgta tcataataat
      841 ttttgtatca attttttctt tgatacttca tattttaatc aatcaaatgt tgattcgtta
      901 cgttttgaca tcattattga taattagttt agttatatat tttttaaaga ttaagaatcg
      961 aattatagat gactataatt cataggagga gatataagtg tttaaaggaa aaacaatatt
     1021 agtaactggc ggaactggtt ccattggaag tgaaattgtt cgtcagcttt taaaatatga
     1081 accaaaagct atcagaattt atagtagagg tgaagaaaaa caattttata tgcaacaaga
     1141 gctctcaaat cacactaatt tgagattttt aattggtgat atcagagact ctcaaaggat
     1201 tgattatgca tgtcgtgatg tggatattat ttttcatgct gccgcaatga aacacgttcc
     1261 agcaagtgaa tataatccta tggaagctgt aaaaacaaat gtgatgggaa ctcaaaatgt
     1321 aatagatgca gcgatcaaaa ataatgtaaa acattttgta ggtatcagta cagataaagt
     1381 agtgagtcct actaacacca tgggtgcgac aaagttattg agtgaaaaat taataatagc
     1441 tgccaatcag tataaaggaa aaacagaaac aatattttct tgtgtaaggt ttggaaatgt
     1501 catggggtca agtggatctg ttattcctct ttttagtaaa caaataaaaa acaataaaga
     1561 tattactttg acagatgttg atatgacacg atttatgatg tcaataccgg atgctgcaag
     1621 attagttctg tctgctgcta tgaattcaaa atttggagaa acatatgtct taaaaatgcc
     1681 ggcaatcaat attttaactt tagctcaatc attaataaaa atatataatg atacacatga
     1741 tgaaaaatat agaggtgaaa taaaagttgt aggtatcagg ccaggagaaa aattatatga
     1801 agagctaatg actacagacg aaattgaacg ctcatatgaa aatgataaaa tgtatatcat
     1861 tccaccacac tatcatgcta ctgacgaact gtacaaagat tatgaaaaaa ttaaaatcac
     1921 tgcatactct tcagatgcaa cagaattaat gagtggacag gaatttatta gaatcgcaaa
     1981 agagaataat gtcgtgactt ggtaaggagt tttatgatga aatcagaagt atatgtaatt
     2041 gcagaaattg gtgtaaacca taatggaagt ctagatttag ctttagaaag tattgataaa
     2101 gctaaagaat gtggagctaa cgcggttaaa tttcaaacat ttaaaacgaa taagctagtt
     2161 tccaaaaagg ctccaatggc aaaatatcag aaggataatc ttaaagagga cagttctcaa
     2221 tttgaaatgc taaaaaaatt agaattatct caatctgatt ttattagaat aaaagattat
     2281 tgtatacaaa ataaaattga ttttttgtct actccttttg atgaagaaag tgcagacttt
     2341 ctaaaacaaa ttggtgtaca aggatttaaa attggctcag gagatttaac aaatttacct
     2401 tttcttagaa aaatagatat gtatgggttg ccagttttat tatctacagg tatggctgat
     2461 attcatgagg tcagtgaggc tgtagattgt ttcatagata gtccagttac tattttacat
     2521 tgtacttcaa attatccggc tagttctgaa gatattaatt tattggcgat taacacatta
     2581 aaagaaatgt tcgggatacc cgttgggtac tccgatcatt cattgggata tgatgttggt
     2641 atttgtgctg ttgcgatggg tgctaaggta attgagaaac attttacctt ggataaggaa
     2701 ctacctggtc cagatcataa agcttcttta gatcctcagg aatttgcaga ttttgtaaag
     2761 catattagaa acactgaaat atttttaggt gatggtgtta agcgagcaat gccttctgaa
     2821 cttagtacaa aagaagtatc aagaaaaagc ctagtggtca ctgagaattt aaatgtggga
     2881 gatattctta cagagaaatc tcttacaata aaacgtcctg gaaatggaat agctcctaag
     2941 tttttatatc aatatattgg caaaaaagtt aaaagaccaa taagtgcaga tagtgttctg
     3001 cttgaggaag atgttgaatg aaaaaaatac ttatagtaag tggaacacga gctgattatg
     3061 gaatatataa acctgttgct gagaatttac tacaagataa ttgggatgta tcatttgttg
     3121 tttctggaat gcatttatcc cataaatatg gatttactaa agaattgatc ttagctgacg
     3181 gatttagaat tagtggagaa gttcaaagtt tgtttttaga gaacaataag ggaaacatgg
     3241 ctcggtctgt ggcattagag ataaatggtt tcacaaatat atttgaacaa actagccctg
     3301 attttgtatt attactaggg gatagaggag aaatgttggc agctgcgata gcttgtttgt
     3361 atcttggaat caaaacagca catattcacg gaggcgaagt gtctggtact gtagatgaat
     3421 caataagaca tgccataacg aaaatagcat ctgttcattt tgctgctacg gaaaaaagca
     3481 aagagcgttt aataaaaatg ggggaagatt cttggagagt ttttcccgtc ggagctccta
     3541 gaatagatac tattttaaat aatagattac ctagttttag agatataaaa gaaaaatatc
     3601 atctaaaatt tgaaaagaaa aaatacgttt tgcaagtatt tcatccagtt gtaaccgaac
     3661 tagaaaatat agaaaaacaa attttagctt tgatcaattc aatggaacaa caatctcaac
     3721 caattatttg cattttacct aatagtgatg ctggtagcga aattatcaga aaaatgtacc
     3781 agaaatattt aaaaacggat attatttata ttacgaattt agagccggag gaatacctga
     3841 ctatattaaa agagtgcaaa tttatgatcg gaaattcttc ttctgggata attgaagctg
     3901 catcattcaa aataccagta ataaatttag gaaccagaca aaatggacga gaacaatcta
     3961 aaaatattat aaatgcaaat ctaaatgaaa atgaaattga aactgctttg aataaaatac
     4021 aagatcctca gtttataaaa agcttgaaaa atgttaagaa catctacggt gacggcaaca
     4081 gttctagaag aataacaaaa acattgaatc aaatattgaa tgatggggaa gatattaagt
     4141 ggattcaaaa acaaatagct tattaaaata
//
LOCUS       DAZUKZ010000163         4132 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000163 DAZUKZ010000000
VERSION     DAZUKZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.169"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1053)
                     /locus_tag="ACG8EF_002698"
     CDS             complement(<1..1053)
                     /locus_tag="ACG8EF_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HFS4947948.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSL"
     gene            complement(1057..1467)
                     /locus_tag="ACG8EF_002699"
     CDS             complement(1057..1467)
                     /locus_tag="ACG8EF_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4947949.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(1485..1976)
                     /locus_tag="ACG8EF_002700"
     CDS             complement(1485..1976)
                     /locus_tag="ACG8EF_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS4947950.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(1986..2777)
                     /locus_tag="ACG8EF_002701"
     CDS             complement(1986..2777)
                     /locus_tag="ACG8EF_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4947951.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(2770..3543)
                     /locus_tag="ACG8EF_002702"
     CDS             complement(2770..3543)
                     /locus_tag="ACG8EF_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4947952.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(3699..>4132)
                     /locus_tag="ACG8EF_002703"
     CDS             complement(3699..>4132)
                     /locus_tag="ACG8EF_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4947953.1"
                     /translation="LINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTKLRFLD
                     SKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHLKEGAQ
                     LFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         1297 a          846 c          555 g         1434 t
ORIGIN      
        1 taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt taggaacaat
       61 tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg gagtgatttc
      121 tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt ctgtcttttg
      181 aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat cgttctcccc
      241 ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc cgactgttct
      301 tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc aatctgaaaa
      361 taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg aaaaaagtaa
      421 ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa attcattatt
      481 agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa aatagaccaa
      541 tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca aaacgtattt
      601 tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat catatccttc
      661 ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat ctttagaact
      721 tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat tatgtccagt
      781 atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa tgtattgatc
      841 aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt tcacaaaatc
      901 agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat ttctcgtatc
      961 tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat ccccaaacca
     1021 aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt cgttagtatg
     1081 tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt cgatgatatc
     1141 atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc ctaaattcat
     1201 gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa attcgttgaa
     1261 aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta cttgagaaac
     1321 tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg ttaccacatg
     1381 acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa tggcaatatt
     1441 tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata aataaaatcc
     1501 tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac tccatcacta
     1561 attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg acggatgcgt
     1621 tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg taaaccgtta
     1681 tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt ttgtccttca
     1741 tatttaccag aaagaatatt tttaactgct acttcccttg taattattga agttctaaca
     1801 ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc tacttcatta
     1861 ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc tacttgtcca
     1921 tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc atccatattc
     1981 aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa gattaataga
     2041 attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc tactacacat
     2101 gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat tgttaagtcg
     2161 ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag tgcaccgacc
     2221 accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt ttcaatttga
     2281 gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc agtaatcctt
     2341 aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc caatgccata
     2401 ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact atctccaatt
     2461 cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa agacttattt
     2521 ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt atacggcgta
     2581 gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg atcatcttta
     2641 tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc cattttttca
     2701 taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt gtcgattttt
     2761 gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta cttgagtatt
     2821 atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg aaatccctaa
     2881 aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg caaagtaatc
     2941 ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata gtccccctgc
     3001 tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc ccactaacgt
     3061 tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg tattaattga
     3121 tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga atttttctgc
     3181 cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta caattaacat
     3241 tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg agattgctaa
     3301 ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac caaaactagt
     3361 aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt gaatatctcc
     3421 cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga ttttgggccc
     3481 ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt gccaaaacgc
     3541 cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg ccaactaaca
     3601 ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct aaatatactt
     3661 tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt ctgctcaaaa
     3721 attgtataga ttaactcata ctctcctttg ggtatttcaa tacagtaaat atctttgatt
     3781 tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa atagttgcgc tccctctttc
     3841 aaatgttcat ttgttattcc atcaccaatc ataattcgtt caatcattcc agaaagatgt
     3901 agaaatagat taattctcag aaaacttggg attttgacat cgtaatattt ttcatacaaa
     3961 tatagtactt tttctatctc attgaatact tttttggaat ctaaaaagcg aagcttagta
     4021 ctagcgcctt caatagtaaa gagtttaact aattcattta agcagaactc acagctatcc
     4081 gagtccatat acttatttaa tttttctaag gtttgattcc cattaattag at
//
LOCUS       DAZUKZ010000164         4124 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000164 DAZUKZ010000000
VERSION     DAZUKZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.170"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            405..926
                     /locus_tag="ACG8EF_002704"
     CDS             405..926
                     /locus_tag="ACG8EF_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947954.1"
                     /translation="MDKPFLDSVREYLSNPKSTLFPHEDYKIIMNYYLPIGAPQNSKI
                     KIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGL
                     DLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLM
                     ELKKYSNTQTKFS"
     gene            1042..1362
                     /locus_tag="ACG8EF_002705"
                     /pseudo
     CDS             1042..1362
                     /locus_tag="ACG8EF_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1540..1899
                     /locus_tag="ACG8EF_002706"
                     /pseudo
     CDS             1540..1899
                     /locus_tag="ACG8EF_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            1963..>4124
                     /locus_tag="ACG8EF_002707"
     CDS             1963..>4124
                     /locus_tag="ACG8EF_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4947955.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNIL"
BASE COUNT         1511 a          563 c          760 g         1290 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa tacgttatgt agtaaatgta atagtttttt tggtagtgag tatgttgagg
      241 aatatattag atttgcagaa gacaataaaa atttccttta taacaataca agtttaaaga
      301 atggtaagtc tgatttaaca cattcaataa agaaaatgaa ttcacttaga gttgcaaaag
      361 caattgtagg aatgtttttt agtttaaatg gggatgaaga ttcaatggat aagccattct
      421 tagattcggt acgagaatat cttagcaatc ctaaaagtac tttatttcct catgaagact
      481 acaaaattat aatgaattat tatcttccaa taggtgctcc acagaattct aaaatcaaaa
      541 ttcctaatga cattgtaaat gatggtgaca ctcacaatgt aataaaaggt tttctgcccc
      601 ttgatctatc aactgggaga aaaattcgat tttcagaaat tcagtatgaa ggaattggtt
      661 ttactcttat agatttaaaa aattcaactt ttgaaataaa agaaggatta gatttaaaaa
      721 atctcctttg tgcagatgat aaaatcaaaa aaatacaatt gtacaaagtc cctatattaa
      781 caccaacagt acttacaatg ttggagatgg aaaataatat taattatatt gactcaggtt
      841 cagatttgct gattaggtgg caggtaataa ctaattttct tatggaatta aaaaaataca
      901 gtaatactca aacgaagttc tcttgaagtt ataaagaata caataaatat aaatttcctt
      961 tagactatat taaaatcagc aatcgaagga aaataaaaga agaaaatggt acgcaaaatc
     1021 taactattag agcaaaaaca ggaagctgct ttggaagata gtcccgaatt gtttccgatg
     1081 ttaaaagatc gtttaaatga attgactgaa aaacgaagac agttacaaat tcgagagaat
     1141 gaaatcttag ggatctttca acaacaaggg gaaccgattc aagtcaaaga tgtccaacag
     1201 atattgacta gtttggattg ctttcttatc cagagtgaga agaagcaagt taaagctttg
     1261 tatcacacct ttattgagaa aatcaccttt gatcctaatc agaagtagat attgccgatc
     1321 ccgatgagat tcacacctac tgtgatttga taattgaatg agtaatatca aatagccgta
     1381 tccaaaccaa aggatatggc tatttttgtg ttagggatat acgtcacgta gcttgttatc
     1441 aagaataagt ttctttattt ttttgctttt tctaaaactg agtatgctaa aataagaggg
     1501 tatatagcaa ctttaaataa gagataggag tgtatctttt tgggtgcaga attaaatcag
     1561 aagttattta gtgcggcaga taatctccgt agtaaaatgg atgcgtcaga atataaaaat
     1621 tatttattgg ggctgatttt ctataaatat ttatccgacc gtttgttgga acaagtggtt
     1681 cttttagcag atgaatcctt agaggaatat gacacagtaa gcaaacaaac gatgttgtat
     1741 cgtgaattac tttcagatga agaatcaaaa gaagatctga ttgcaacaat cgttgatatt
     1801 ttggggtatg cgatttcgcc agagtatctt ttcaacgtct tagcggatca agcaaaacaa
     1861 gcaacgttcc aattgaatga tctaaacaaa gcctttttag caagtcatat ttatggaaaa
     1921 atactgtcgg caaaattcga atagtgtcga ggaggtgctg tgatggaaga attaaaagaa
     1981 cgcattgtca attatgtcat agagaactct gagattaaaa ataaatgggg gaacaataca
     2041 gcacagaaaa tatctgaaaa attaaatgtt agtcgtaata ttgtctctca atatttgaat
     2101 gaattcgtaa aagatggaga atttggaaaa atcaatactc gtccagtaat cttcttttct
     2161 ttaccggaga aagttccaaa tcaacaaatt tttagctctt cagaggaagc ggaaaaatgg
     2221 tatgatagct cttttagggt tggaatagaa agggttatcg gaagtcaagg aagtctgcga
     2281 aatgtaattg ctcaaattaa agcagcaatc tactatccgc caaatggctt acccatttta
     2341 cttcatggcg agactggaac tggaaaaagc tatttagcaa attatgtgta taaggatttg
     2401 ttggccaata attatataga agagaaggca tctttcattt cagttaattg tagtgaatat
     2461 gccaataatc cagaactttt cttgtccaat ttttttgggc atgtaaaagg tgcatataca
     2521 ggagccgaag atggaagaaa gggtttagca gaattagcta ataatggagt cttattttta
     2581 gatgaaattc atgcattgag caatgaatgt caggagaaac tttttcagtt tatggatacg
     2641 aggaaatttc atcggatggg tgataatgaa aactggtaca catcaagttg ctggattata
     2701 tttgcaacat cagaaccacc tgaggataaa ctattatcaa ctttgttgag aagaatacct
     2761 gtaaagctaa cacttccaac attagacagt cgttctcttt atgaaagaaa acagttaatc
     2821 aagatatttt ttaaatcaga agaagaaaga ctagcaaaga aaatactgat atctcaacaa
     2881 ttattagatt ttttattaaa ctatcgcttt agtgaaaatg ttggcaattt gaaaaatatc
     2941 attcaattga cctgtgccaa tgccttttat ttcttagatg atttggaaga actttatatt
     3001 cagatgtatc atttaccaca atgggtgaat ttcaacggca tgaaggttat gaaaagacag
     3061 aatgaattaa aatatattca aatggatgat ttggaaaaag tagacgaagt ttttaatatc
     3121 aacgaactta tagagtatct ttatcaaacg tggaagaaag acactgataa gcagcagctt
     3181 tttgagaaat atgatagata ttttagacaa aagaatgttg atatccaaaa taattcagct
     3241 ttaacatatc ccacctatca agagattcga tttagacagt atgttaagga aatagagaga
     3301 gattttcaaa tagaatttga ctattattat cttcaaataa cgtctatctt tttgaattct
     3361 tggatttatc aaatccaatt aacatcggac gataaattat attccttcat caatgatatt
     3421 aatagttggt gtagtcgaga agttcaatta gtcaatcgtt ttattcaaat aggggatatt
     3481 tatttaacaa aaattcaaaa aaatgttttg gttattttat ttaagttaat gtgtagtaaa
     3541 gaatttcagc aaaaacgttt agcactgatt ttggctcatg gagatctaac ggcaacctgt
     3601 atagcaagta ctgctaatag gatcgttgag agatatatat acgacgccat tgatatgcca
     3661 ctaatgactt caagtagtga gattgcagac aaaattaata gatacttaca agagtgtacc
     3721 ggatatgaag aaattatttt acttgttgat atgggttcac tggagaaaat ttatctaaat
     3781 ttggaactag acgctcaaaa aaaattcgga ctactaaaca atgtttcaac aagaatggct
     3841 attgatgttg caaatcagat attattagat ttaccgctta aacagattct tgaacaagta
     3901 aaagaaaatc aaaatgtgga ttatatttat gaatcatcaa atattaagaa ggatatgatt
     3961 gtctgttctt gtgcaagcgg attaggaact tcgtttaaat taaaaaagat acttgaagat
     4021 agctttccaa gggatactga tcttgtcata gaaacgtgtg actattactc tttagtgaat
     4081 gaaaactatt tatcggagct tcgtaaagag aacaatattc tttt
//
LOCUS       DAZUKZ010000165         4078 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000165 DAZUKZ010000000
VERSION     DAZUKZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.171"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..643)
                     /locus_tag="ACG8EF_002708"
     CDS             complement(101..643)
                     /locus_tag="ACG8EF_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFS4947956.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            778..1167
                     /locus_tag="ACG8EF_002709"
     CDS             778..1167
                     /locus_tag="ACG8EF_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947957.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1171..1935
                     /locus_tag="ACG8EF_002710"
     CDS             1171..1935
                     /locus_tag="ACG8EF_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4947958.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2064..3011
                     /locus_tag="ACG8EF_002711"
     CDS             2064..3011
                     /locus_tag="ACG8EF_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4947959.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3141..3881
                     /locus_tag="ACG8EF_002712"
     CDS             3141..3881
                     /locus_tag="ACG8EF_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947960.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1359 a          689 c          821 g         1209 t
ORIGIN      
        1 tgatgagtat atgtcgatgt tttttaatca aaataataaa taaaaactgt ctaacattta
       61 aaatttaatt tagatattag acagttttta agtttgtgag ttattttcct tgtcgttttc
      121 tcactttttt agtccagaaa ccacctgaaa tatattttgg ctcataagaa atgataaaag
      181 cacgctcttc gatttctttg atcaggcgat acagttcacg ttcgcttttt ctaggagaaa
      241 gtatttcgag aactagccgc tcaccttcca gccccattgc agaagtctgt gtcacaccat
      301 aaccgtgttc tctcagtata cccggcaaac ttttatcttc tgcgctattt gctggcaaaa
      361 tcgttgtgac catgatgtat cctaatgcca atttgtcttc aatcaaaatt ccgacactga
      421 tacctactgc gtaacctaac gcatagacaa ataaattcag tggattatct aaacgatcta
      481 aaacaaggga taagccgacg atataaatcg tgatctcgac catactgaca agtggagcaa
      541 tcagtcgata gcctttcatt gtcaacatga aacgtaacgt gttcagagtg atataagcaa
      601 aattaatgag gaagatcatc cccaacattt ttggatcaat catttgtaaa actcctttca
      661 tttatataac aacaatatca aaaaaaatcg taagtgaaga aaaattgaaa ctttatacat
      721 acaaaaaaat gataaaatga ttataataga attataaaga taggttaggt gattaaaatg
      781 aaaacggtca atcgtgtgtt ttgtgccagc cacgtgctaa gtctccttct cagtatatac
      841 ttaggtgtct attcgattca acagccaatt gaggtagaga acattgtttt tcagatgtcg
      901 ctgacagatg gtttaatttt aagcgttttt ttctttttga tactgtttat tggtaatatt
      961 ttcggtagcg ttgccagttt tttgaatttt tctatttatc cactcctttc gctagcatta
     1021 ggagtggtgg gactttgttc tctgtgctta tttcccgagc cattcagtcc tgcatttata
     1081 ttatttggta cactgaacct gttccagaca actgttggag catggttgct atggcgatca
     1141 ggaaatttaa tgaaaatagg tgagtaacaa ttgaatccaa ttatattgaa acgttctgtc
     1201 catcatattc cagtgttaga agttgttctg aaagaattaa gagaggaacg tattcccgtt
     1261 gtcatctatt atcacggatg gcagacaagt aaggagttag ttttgacgca aggaagaaaa
     1321 attgcgcagc agggcattcg tgtgatcctg ccagatgcga tgaatcatgg ggagcgcaaa
     1381 cagccggttt cttctattcc atcctataca ttttgggata gtatctacgg aaatctgttt
     1441 gaatttgaca cacttatcaa gcatttagaa aaaagaggtc ttctacatga tcagcttgca
     1501 gttggcgggg tttcgatggg gggaatgaca acatgcgcct tgttggctaa acatcctcat
     1561 atagtagcgg gcgtgtgttt gatgggttcc cctgcacctt tggaatatgg aaaagaaatc
     1621 gtaaggcgcg catcggaata taattatgtc gtaccaaaag actattttga tttgatttca
     1681 tgggttaaga gctatgattt gtcattgcag ccggaaaagt tagctggacg tcctctgttc
     1741 ttttggcatg gacaagctga cgaaaaaatc ccttatatac aaacggaacg atttgtagaa
     1801 aaaaatcggc ataagtctta cggaaaagaa attgtttttc gttcctcaaa aacacagcga
     1861 catatggtac aaataccact aatggaagaa gcttccactt ttttagcaaa aaatttgata
     1921 aaaaagtata aataattgaa acggaaaatc actcatgacg atagaggatt ttccgttttt
     1981 tattttgggc tataataaac tctgttagga aaatttggta taattgatag taagaatgac
     2041 aacatgttag aaaggaagag agcatgccca ctttagcaga tgttgcaaaa cgtgccaatg
     2101 tttcaaaaat gacggtctct cgagtcatca atcatccgga acaggtgact gacgaattga
     2161 aagagttagt tttctcagca atggctgaac tggattaccg tccaaatatt gctgcaaaag
     2221 cacttgtcac gaatcggtcg cagatcatca aattatttat actggaagaa atcgacacaa
     2281 ctgagcccta ttacatgaat ttgctgatgg gaatcgctaa acggatcggt aaagcgcatt
     2341 attctctcca attagttaca aatgatgcct tcgacgtcgg ttcttgtgat ggatacatca
     2401 tcacaggtgt cagaaaaaaa gacttcgatt ggatcgaccg tttggaaaaa ccagttgtct
     2461 tgtttgggga aaattctgaa ggatatgatt atgtagacag cgataatgaa tatggtacag
     2521 caaaagcaac gcaatatgcc ttagaaagag attatcaaac gattatttat atcggtatcg
     2581 aagtggacga gccttttgaa atttcaaggg aaactggtta tttatctgtg atgaaggaag
     2641 ttgatcttcc tagtaaaatc ttgcggtttc caaatcattc gacagaagta gaaagatata
     2701 tgacagaaaa ctgggcagat tttacagata aaacttgttt tgtatgtagt tcagatcggc
     2761 tagcactagg agcagtaaga gggattataa atgctggagg acacttgccc gaagaatttg
     2821 gtgtgattgg atttgatggg gtatttttag accaaatcag ttcacctaaa ttgacaacga
     2881 tcaagcaaga tattatacgt atgggtgaag tatgtggaga aatgttgtta aaaaaaatag
     2941 aagaaaaagg ggcgagtcaa ggttatcgtc gtttttttcc tgaactagtt atcagagaga
     3001 caacgaatta agtcttatta ctatatatat atttatgaaa ttcatcgcta ttttcttaaa
     3061 ataatcagcg cattgctact gaaaacaatg aatctgtgct attattgaga aaagttccat
     3121 ttttgagaag gatggtttga atgaaagaaa tcagacgaaa acatttccgt tttcctgcgt
     3181 acgatgatga gttaggcgta aaattaacca acgacaagaa gcgagtatta tttgacggac
     3241 aagaagatct tttgactgaa cagaatcagt ccccgtttga acagatgaaa gaatttcatt
     3301 tcacgaacac gaacaccgag aaaatccgtc gcaagcgtcg tgcaacaaat ggattgaagt
     3361 cagctccgcg taaggaaaat ttggaaaagc ataaaggaaa attaccggat tataccggcc
     3421 attaccaatc agaaatcaca tcgacaggac gaaaaaaact ttttggtgat tcctctcgtt
     3481 ctgtcagaca aagtgaaaca aggacagagt ccaagcatac tacacgaaac gcatctgttg
     3541 acacgaaggt gcgggagcgt tcttattttg ttcctaaata tgtacccgca tcgattatcc
     3601 cagaccctag agaaagcaaa atttctgagg atcatttaat cgaatcgatg aaaaaaacgg
     3661 atgactctta tctgttgttc gatacagaac cgacgccgta tcaagaaaaa agaaatggtg
     3721 agccgtctgt acgaacattc aatcgaacaa aaacgttaga ggaaacaaag cagtcagaag
     3781 aaaaaaaatc gaaaaaaagc catggtatct tagaacgctc attacaagga ttgatcgatg
     3841 atcaaggaaa ctctttggga gaaaatagct attttaaata agtgatatac aaatttatat
     3901 tcggctacag ttggaaagga aacgctgcgg cgtttcctta ttttatactc tatccgaaag
     3961 tttatatcgt atcctcaaat ttttttgagt ttaagcgaat tttttctccc aataagtaag
     4021 aaaataatgg caaaataaaa aaacaaacta ttttcgcaga tcgtttgcaa aaatagtt
//
LOCUS       DAZUKZ010000166         4036 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000166 DAZUKZ010000000
VERSION     DAZUKZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.172"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(242..391)
                     /locus_tag="ACG8EF_002713"
     CDS             complement(242..391)
                     /locus_tag="ACG8EF_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947961.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            853..1065
                     /locus_tag="ACG8EF_002714"
     CDS             853..1065
                     /locus_tag="ACG8EF_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947962.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1343..3142)
                     /locus_tag="ACG8EF_002715"
     CDS             complement(1343..3142)
                     /locus_tag="ACG8EF_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFS4947963.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3266..3862)
                     /locus_tag="ACG8EF_002716"
     CDS             complement(3266..3862)
                     /locus_tag="ACG8EF_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4947964.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          689 g         1187 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct
      541 attttctttt aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat
      601 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
      661 acaacatagg aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata
      721 gttatctaaa aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt
      781 tcaaatacaa ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa
      841 agagggaatg aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga
      901 gtatctcatg aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat
      961 gttgaaagtt atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc
     1021 gtaaaattct gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga
     1081 attcgcaaga actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa
     1141 aaagaaaaag ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta
     1201 ttttagctat tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg
     1261 tcaaacaaaa aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac
     1321 ttttttcatt aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg
     1381 aataagtgat ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg
     1441 ttatgatacc atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg
     1501 tccatacata tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg
     1561 tactatattc cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag
     1621 gtaagatgta gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt
     1681 acgtcaaact ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa
     1741 atcgttcgac aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt
     1801 ggtttagctt atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc
     1861 cgttttcagt aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat
     1921 ttacccctgt taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg
     1981 tttcctgaac atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg
     2041 cttctaaagt attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg
     2101 gttttgtctg aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga
     2161 aaaaaactgc aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg
     2221 cttgtgggat aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa
     2281 caatgatata aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta
     2341 aaagcgtcat agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca
     2401 tactttgtgc caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc
     2461 caatcacaat aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt
     2521 cccgaatggc tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca
     2581 gtcgaatccc aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc
     2641 aacgattagc aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat
     2701 cagaattaaa acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca
     2761 aaaaagtaat gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca
     2821 aagagattgg ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga
     2881 aataaactaa cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat
     2941 gttgagaaaa aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta
     3001 aaataaaacg ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca
     3061 tcagcaagac atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta
     3121 agctacgttt caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat
     3181 gaaaaaggaa agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc
     3241 aatgactaat tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat
     3301 aaatcaaggc catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg
     3361 tatatcgttt cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag
     3421 caactggaag aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc
     3481 agccaatata tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat
     3541 aacgacgccc tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga
     3601 taaatccagc ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa
     3661 tgattggaat aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga
     3721 tggaatcacc tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa
     3781 aataaatcgt cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt
     3841 ttcgagaggc agcaatattc attattcatc aactccttag aggttctttg ttaactatac
     3901 tttactagaa aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg
     3961 taagttcatc ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt
     4021 aataacttgt tttttt
//
LOCUS       DAZUKZ010000167         3925 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000167 DAZUKZ010000000
VERSION     DAZUKZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.173"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(573..1130)
                     /locus_tag="ACG8EF_002717"
     CDS             complement(573..1130)
                     /locus_tag="ACG8EF_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947965.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="ACG8EF_002718"
     CDS             complement(1309..1920)
                     /locus_tag="ACG8EF_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947966.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="ACG8EF_002719"
     CDS             complement(2033..2290)
                     /locus_tag="ACG8EF_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947967.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="ACG8EF_002720"
     CDS             complement(2887..3165)
                     /locus_tag="ACG8EF_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947968.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="ACG8EF_002721"
     CDS             complement(3199..3450)
                     /locus_tag="ACG8EF_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947969.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DAZUKZ010000168         3884 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000168 DAZUKZ010000000
VERSION     DAZUKZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.174"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..283)
                     /locus_tag="ACG8EF_002722"
     CDS             complement(<1..283)
                     /locus_tag="ACG8EF_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS4947970.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENI"
     gene            complement(732..2140)
                     /locus_tag="ACG8EF_002723"
                     /pseudo
     CDS             complement(732..2140)
                     /locus_tag="ACG8EF_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2361..2723)
                     /locus_tag="ACG8EF_002724"
     CDS             complement(2361..2723)
                     /locus_tag="ACG8EF_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947971.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2727..3035)
                     /locus_tag="ACG8EF_002725"
     CDS             complement(2727..3035)
                     /locus_tag="ACG8EF_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947972.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3044..3463)
                     /locus_tag="ACG8EF_002726"
     CDS             complement(3044..3463)
                     /locus_tag="ACG8EF_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947973.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3456..3689)
                     /locus_tag="ACG8EF_002727"
     CDS             complement(3456..3689)
                     /locus_tag="ACG8EF_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947974.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3686..>3884)
                     /locus_tag="ACG8EF_002728"
     CDS             complement(3686..>3884)
                     /locus_tag="ACG8EF_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS4947975.1"
                     /translation="LYDHSEWGNLAKNTKKKYRDLLKQESQNDELTNHLRETFAESAD
                     DLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1089 a          863 c          551 g         1381 t
ORIGIN      
        1 caatattctc ttcatgttgg tctaacaact gccaaccatt attactaaca gatcttaaat
       61 aaccattacc ttcatttagg cgcataagct gttcaaaatt taaaatcaac acatcatttg
      121 gagtgtgata ataaatattt tctttcagtt ttccgactat ggtaattcgg tcaattgacc
      181 actgcatttt taagggaagg gtcaaacttt gatactttaa ccgcctgtta gagggggctg
      241 ttaatttttc ccactcattt atgatcgtgc cttttttact caaattttgt tcctcctcaa
      301 agaaaaagag acttctggaa agaattttca aaagttcacg taactcttaa aaattcatta
      361 ccaaaaaatc tctagtcttg aagaaatcta aaaatattcg tataatgatt aagaagttac
      421 ggagctagtg cgattagttt ccgtaaaggt cgagaacgtg tccagcgttg ctcgacttta
      481 tttttttgct cttttttcag tttatttatt tgataactca ataatagcac ttctctttag
      541 ctatgcaaat atccaaaaaa taaaaccgca ccctttgaaa agggcgcccc caaacttcgc
      601 taacgctagg ttggccaaaa aatggacttg ccatttttta gcaaatgtca gcttcaggga
      661 ttagtggttt agccgtcaac tccttatcct cggcacaaac cgcctccggt accgttgact
      721 gcacatcgta actaccactc gatctcatct tcagaacgct ctaatttacg ctgtgagacg
      781 ttttgacgat aaaataaggt tttctcctgc tctaacactt ctaattcctc agaaagcccc
      841 atatcctttt ctgcaagacc aaaacggata gacaaacgag tgatcgattt ttgaaatgcg
      901 ttttttaatc gctctaaacg ctggcgtaaa gagcgatttt cttctttcag ctttttattt
      961 tccacttgta aatctgctaa ttgttttcta ccagacaaat gacgctcttc ttccaatgta
     1021 gctacatact catttaattc tgtattttgc tcaagcatct gtaaatattg ctcactcatg
     1081 ttatcagcat ttctcttggc atttgttgct ctcgtagctc gttgatacaa atcaacaaaa
     1141 tcattcccag ataaatgata cgaaccatca ggaagcctct tgaaactttc taaaggaaca
     1201 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
     1261 tcttcaatct tcactctctc agaagccacc agagcctttt ctcgctcatt ttcttctctt
     1321 tgcctagaaa aatatacctc aactcatcag aaacgctctc acgctctttt acggccgttt
     1381 gacgcaattc tgaaatctcg ctcatgattt ctttatattc gtgaacatct ttgattttgt
     1441 tagtaccgac taactcacgc tcccaaccaa aatctgccat caaacgttca attgactcaa
     1501 cttcctgatt cctaaaatta ttccaaatca ctcgaccatt ctctttatct gctcgtaaac
     1561 cctgctgttg caaggctttg ttaaaacttg gttgcttttg aacaccccgt ttataccctt
     1621 cagcaacagg aacaatattc aaatgcaggt gcggacttac ttcgtcatta tgaataacag
     1681 cattgtagac ggataaatta ggattccgtt ctttaaattc tttgaaatat tcaactaaaa
     1741 ttttgttggc aatcactcga ttttgttctg cttgttgttc cgatatgcca gtagaagaac
     1801 catcatcacg agtagtacga aaatcatcaa gagtaccaat ttgcacgata aattctcgct
     1861 gtggttccaa ttttttatcc ttgcgaacct ttgctaaata gtcctcgatt tttctatcgg
     1921 ctcttttctg ctttgcatta tatttttcta cggcttcacc gaataattct tcatattttt
     1981 cacgaatatt tgtttgttct aaatagatat tttcatccga ttttgtgaaa tcaatatgct
     2041 tatgccagtc attgattttt tggctatcag ttaactcacg attattatga gaaatgcttg
     2101 tattctttgt tccctttttg aacgaaatac tgaaccccaa gttaaaaccc tccttcggct
     2161 gtcgctaata aaaagagtgt aaacctttct gttactcttt gtcaaagagt aacattttcc
     2221 taattgtgac atggctgtca caattccaaa tgctctccga gggtattcgc tgtcacaagt
     2281 gacaacgaaa aaaggagccg aaagcgtgtc acgctctcaa ctccttttac gctttatttt
     2341 gcttcacgca aaataatcgc ttatcgtaaa ttcacgtcat taaatattga cgtaaattta
     2401 ctctcactca tctgactttt cttattcggt atcactccga ataagccgtc agattcatcg
     2461 ggataatctc gttcgcttgg cgctgatttt tcatcagcaa gccactcatt atccactgat
     2521 ttttcgtgtt gctgatcgtc agaaatcgac gatagccacc gctcaaaatc agcaaaagtt
     2581 tcaaacccaa aacgcttccg aagagccttg cccattgcag cattttcttt ctcaatcaag
     2641 cgttttttct gttggcgaag tttcgcttgt tcttttttta atttttcttc ttgcgcttta
     2701 atatctgcta aagtcattcc cataaactac atctcctttt ctttggaatt aataacggaa
     2761 atttggttta actgcataac atgactcatt aatttcaacc aaaattcttt ttctgattca
     2821 tcagttgttt gcgctaaatg cttatgaaca aattcattag tcaaaactga agattttgct
     2881 aaatcagcat tgataaccga gttgagaaca tccataattc gttcaagcat ttcactataa
     2941 ttatcttcat ccggcaaatt caaataatca gctaaccgtt catacatcaa ttcaattcta
     3001 tcgactgttt cttctctcaa atcaatttta tccatgtcac acctcacttc cagaattgcc
     3061 accattttcg attttttaat tcttgatttt ctagtaacaa ctgatcgttt ttttgttgct
     3121 gaagcagggt caatttttgt tgatcacgaa tcatttctgt taattcagaa atatgtttgt
     3181 ccttctcagc taattgatca cgcaaaaact cattctcttt ttctaaagga aaatcactgc
     3241 ctgtgttatt gaccggtaat tgaccagtca attgatcact ttttgaccga taaattgacc
     3301 gaacaacacc aatcaactct ttatctagca tgataatgcc attttcttta tactgagcaa
     3361 ggtcaaaccc tgaattgacc agttgattgc gcactgtatt tttcgataca cccaattcgt
     3421 cagcaagatt ttttatggtc atcttttctg acaaatcagt cattagcttc tccactactt
     3481 tcagctaccg cataaatata tttaacggcg tgcttaggga taataatcgg agagtcaatt
     3541 ttattatttt tcaaaatcaa ttcatattgc gttacatcaa gtaacttacc ggacattttt
     3601 ttgctatcca aaaaaacaac cacaacgtaa tcacctgtta aatttaccaa ccaatttttc
     3661 ttaccaaaat acttttgttt ttctgtcatt tttcttcctc ctctgttacg tctagcccta
     3721 gaagccatgt gtctaattct ttttttaaat cgtctgccga ttccgcaaag gtttctcgca
     3781 aatgattcgt caattcatca ttttgacttt cttgcttcaa caaatccctg tattttttct
     3841 ttgtattctt agctagattt ccccactcag aatgatcata aagc
//
LOCUS       DAZUKZ010000169         3876 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000169 DAZUKZ010000000
VERSION     DAZUKZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.175"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..2257
                     /locus_tag="ACG8EF_002729"
     CDS             158..2257
                     /locus_tag="ACG8EF_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFS4947976.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="ACG8EF_002730"
     CDS             2453..3316
                     /locus_tag="ACG8EF_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFS4947977.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3876
                     /locus_tag="ACG8EF_002731"
     CDS             3421..>3876
                     /locus_tag="ACG8EF_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4947978.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         1544 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa gaaaaa
//
LOCUS       DAZUKZ010000170         3844 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000170 DAZUKZ010000000
VERSION     DAZUKZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.176"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..909
                     /locus_tag="ACG8EF_002732"
     CDS             <1..909
                     /locus_tag="ACG8EF_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947979.1"
                     /translation="LLDDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELII
                     GQTYTITLKGTKPASQTFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDL
                     RIFQLPKETAGACQIDWLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYT
                     EKGLNDTVSLTEPQSIEGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKL
                     YREGKTVTIYFFALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSAYSFVITYSI"
     gene            923..1072
                     /locus_tag="ACG8EF_002733"
     CDS             923..1072
                     /locus_tag="ACG8EF_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947980.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1069..1515
                     /locus_tag="ACG8EF_002734"
     CDS             1069..1515
                     /locus_tag="ACG8EF_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947981.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1517..1654
                     /locus_tag="ACG8EF_002735"
     CDS             1517..1654
                     /locus_tag="ACG8EF_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS4947982.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            1691..1984
                     /locus_tag="ACG8EF_002736"
     CDS             1691..1984
                     /locus_tag="ACG8EF_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947983.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1981..2205
                     /locus_tag="ACG8EF_002737"
     CDS             1981..2205
                     /locus_tag="ACG8EF_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFS4947984.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2202..3221
                     /locus_tag="ACG8EF_002738"
     CDS             2202..3221
                     /locus_tag="ACG8EF_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS4947985.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            3410..3483
                     /locus_tag="ACG8EF_002739"
     tRNA            3410..3483
                     /locus_tag="ACG8EF_002739"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3444..3446,aa:Ile,seq:cat)
BASE COUNT         1324 a          669 c          790 g         1061 t
ORIGIN      
        1 ttactagatg acccttactg ggtaggtaaa acatcgttgg gtgagaatat tgcaaataaa
       61 ggtacgacgt tcccaattaa atctagcgcg tacgaaatat ataaaggtaa cacggaagaa
      121 gagcttataa taggtcaaac gtatactatc acgcttaaag gaacaaaacc cgcaagtcaa
      181 acctttgtag catataatta ttggaacgtt aattttggag aactaaaacc agttgaggga
      241 ttgacagacg tatggtctct aacattcaca ccaacgaaac ttgaaccggg tttgcctaaa
      301 gaccttcgca tttttcagtt gcctaaagaa acggccggcg catgccaaat tgactggctc
      361 aaaattgaaa aaggcgacac ccgaaccccg aatattagtc agtttaaata ctttggtgaa
      421 ggattgaaag atagcaacaa tccgaacgac tacagctggg acatcacgcc tgaatatact
      481 gaaaaaggct tgaatgatac ggttagtttg actgaaccgc aatccatcga aggattgaaa
      541 aatttcgaag atggtattca atcaaaagga aaatctgtat tgacatcaga cgacaacaaa
      601 tatgaagtcg taaccttaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
      661 gaaggaaaaa ccgtcactat ttattttttt gcgttgaatg ggaaaagcag tggtggaaat
      721 gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg
      781 gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga
      841 gccattaaat ggcgaagaaa ctcaagttat ggatcagctt attcatttgt tatcacttat
      901 tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc cttacggatt
      961 cgagcaaatc attgtgaatg acgaagaaca tttaccggaa gaatgcacag aagtcgaacc
     1021 accgattcca aattggaaac cgaaattcaa ttactgggag ggaaataaat gaaaaacatt
     1081 tggaaatatg gccgtactgg cggagagtat gcaggaaaag tattggacga catgcttgta
     1141 tccgttcctt acacagatca gccaccgctc gaaggggttc gttcagatgg cgaaccgcta
     1201 acgattgctg atcagatgtt tgatcctaaa ttgaaccaat ggattatttt agcgaacgcg
     1261 ttagatcaca acgatttaaa caatctcaaa gcgatgtacg aggctcttga acatgaaaac
     1321 ggcaacctaa aacagctaaa tgccaaactc atgctaagcg atgtagcgat taaacaggaa
     1381 aatactgcat tgaaagaaaa agctgacagt ttagcacaaa tcaattcaaa aatgatgctt
     1441 gcttcgttac aaaatagcaa agacatttca gaaattaaag agcaactaaa tccagcttca
     1501 aaaggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
     1561 ttttactgac gatcaagttc gactattcgt tccactatgc attacagacg aagaagcaga
     1621 taaaatcatt aataaagata agagcgcatc ttaattgatg cgtttttatt taaggtaaag
     1681 gagttgtcac atgattaatt taggggaatg gggaatgata gcaggatcaa taaccgctat
     1741 cgtttctttg attttattag taattagacc gattgctgca tctttctcga aaattactga
     1801 gactctttca aaagtaagcc acaatttaga tttgctgact aaagatttag aatcgagcaa
     1861 atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca
     1921 tacagaaaaa ttggtggaac acacgcaaca aatcaaaact ttgtttaggg agagaagaaa
     1981 atgaataata aaacgttcga agtactaaaa tggttcgcac tggtaattat tcccgcacta
     2041 gctactttcg tggggttagt tggtaaagcg ctcaattggc agtacacaga tatctgtgtt
     2101 gtcatcatta ctggttttgg cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc
     2161 tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta
     2221 ttactgcgat gagcctgtta acggctcttt ttttattgcc aattaacgga tttgcctata
     2281 ctatcaacaa tgaatttaat ttgggcgcaa atgaaggtag ctcacaagta gcaaataatc
     2341 agtatatttt actgcatgaa acggctaatg aaacagcaac aggacgcaat gaagcgcagt
     2401 atatgcaacg ttcatggact agcgcttata ctgcttatat tgtgggagac ggcggaattg
     2461 tttaccaagt cggacaacct ggttatgtac agtacggtgc tggttcatat gctaatgcca
     2521 atagtcctgt gcagattgag ttacaacaca cacatgataa agcaactttt gaaaaaaact
     2581 ataaagcata cgttgaattg gctagagatt cagcaatgaa atatggtatt ccattaacat
     2641 tagacacgcc ttataaccaa ccaggaatca aatcacattt atgggtaact caaaacattt
     2701 ggggcgatca cactgatccg tatggatact tatcagaaat gggtgtaagt aaggaaaaac
     2761 tggcttacga tttagctcat ggatttacag acgaaaaccc aacgacttct gagaacaagc
     2821 ctgtcattga tccaacccga gcaggtgcag caaatccaac tttatcagat ggaacgaacc
     2881 attctcacat tgatcagttt ggagaaatcg aaaatgcgaa cttacacgtc gctggatggc
     2941 acatcgctaa ctataagtac gagtatattt tcattatgga ctacaatact gggaaagaat
     3001 tagctcgagt aagagctgat ggaatttata gaccggatgt aaatcaagct tataatactt
     3061 ctggaaatgt tggctatcat gtatctttta acatgcgtaa cttccctaat aagaaagtct
     3121 atgtcatgat gcgggcaacg aatgatccag agggaaacac taaaggcggt gcgcaagatt
     3181 tccatgacaa acgttggtat ttaaatattc ctaaacgata aaaatagctc ctcgttgagg
     3241 agcattacat aactatattg acaactataa aaatcattcg ataaaatagt gatgttatcg
     3301 catatcttca ctatcaccca taatagtcac actccaagct atgcgataac aggtttgttg
     3361 ccacacattc tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc
     3421 tcagttggtc agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacagggt
     3481 acattaacgt agccatttga atcgttctgt gttagaattt tttgaagagt attatacaag
     3541 ctaaagcttt tcttcattgc cactcaaatg agtggctttt ttatgtatcc ttttatggat
     3601 taatgaaagg atgtttcaca tagttatatt tctgtatatt tgaaaagttt tactttgatt
     3661 tttaaataga aagacatttg ggttatattg tgagataata ataaagaaga gtttaaagcg
     3721 ttccccaaaa accactgccc cataagtgtg ttacgcttta aactctttta tatttgaagc
     3781 cattaaaaag cataccatat ttttgaaaaa aagtgagaaa aaaggcttat aattggagtg
     3841 gtag
//
LOCUS       DAZUKZ010000171         3792 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000171 DAZUKZ010000000
VERSION     DAZUKZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.177"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1317
                     /locus_tag="ACG8EF_002740"
     CDS             <1..1317
                     /locus_tag="ACG8EF_002740"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFS4947986.1"
                     /translation="PEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVD
                     LQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGE
                     FYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQEL
                     NTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSAD
                     TTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIR
                     QKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNK
                     LSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHV
                     GNDIKKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1307..2524
                     /locus_tag="ACG8EF_002741"
     CDS             1307..2524
                     /locus_tag="ACG8EF_002741"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS4947987.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2537..>3792
                     /locus_tag="ACG8EF_002742"
     CDS             2537..>3792
                     /locus_tag="ACG8EF_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS4947988.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT         1309 a          653 c          791 g         1039 t
ORIGIN      
        1 ccagagtatc ttttcaacgt cttagcggat caagcaaaac aagcaacgtt ccaattgaac
       61 gatctaaaca aagcctttgt gcaattggca tctacctaca atcaatttaa tgggttattt
      121 gatgatgtgg atctccaatc aaaaaaacta ggaacagatg agcaacaacg aaatgtgacg
      181 attaccgaag tcatcaagaa attgaatgat gtagatgttt tagaacacga tggggacgtg
      241 atcggggatg cgtatgagtt tctgatcagt caatttgctt cagaagcagg gaagaaagcc
      301 ggagaatttt acacacctca tatggtttct gatatgatgg cgcaaatcgt aacgctcgat
      361 caaaaggaac gacgattctt tagtgttttt gatccaacga tgggttcagg ttcattgatg
      421 ttaaacgtac gtaactactt aacacaccca gataacgtta aatatcatgg gcaagaacta
      481 aatacaacga catacaatct agcaaaaatg aacttgattt tacatggtgt cgacgccgaa
      541 gaaatgaatc tccgcaatgg ggatacgttg aataaggact ggccaacaga tgaaccttac
      601 acatttgatg cagtagtaat gaacccacct tattcggcta attggtcagc agatacgact
      661 ttcttagatg attctcggtt taatcgttat gggaaattag cgccaaaatc caaagcagat
      721 tttgcgtttc ttcttcatgg gttctatcat ttgaaggaaa caggtaccat ggccatcgtc
      781 ttaccacacg gagttttatt ccgtggcgct gcagaagggg tcatccgtca aaaattacta
      841 gaagatggca gtatttatgc tgtgatcggt atgcctgcga acttgttctt tgggacatcg
      901 attccaacga cggtgattgt gttgaagaag aatcgtcaaa ctcgagatgt attattcatt
      961 gatgctagtc gtgaatttgt taaagggaaa aatcaaaata aactatcgga agaaaacatt
     1021 caaaaaatcc tcgaaaccta cgctgaaaga aaagatgtcg agaaatacgc tcatttggca
     1081 acctttgatg aaatcaaaga aaatgactat aacttaaata tcccacgcta tgtagatact
     1141 ttcgaagaag aagaaccaat tgatatggtt catgtcggaa atgacatcaa gaagatacga
     1201 caagaacagc aagtgctaga aaaaaaactt ttagaagcta tctcttccct gcaaacaaca
     1261 cctgaaaatg aagcgtggtt acaaggtgcg ctggaggtgt tcaaacatga gcaatgatac
     1321 acaaccagaa atccgtttcc caggatttac agatgattgg gaacagagga agttggggga
     1381 cttcgttgta gattatgttg aaaagacaag tgtacaaaat caatttccta tgctaacttc
     1441 atctcaacaa aaagggatag ttttgcaaga agactatttt gcaaatcggc aagtaactac
     1501 agaaaataat attggttact ttgtactacc tagaggatat ttcacgttcc gtagtcgaag
     1561 tgataatgat gtgtttgtgt ttaaccgtaa tgacattatt gatagaggga ttataagcta
     1621 tttttatcct gtatttactt taaaatctgc tgattccgac ttcttcttaa gacgtattaa
     1681 caatggaatc caacgacaat tatctattca agcagaggga acaggtcaac atgttctgtc
     1741 tctaaaaaag tttaaaaata tagtagcaat gtttcctagt gaggaagaac aacaaaaaat
     1801 cggcaccttc ttcaaacaac ttgacgacac gatcgctctt catcagcgta agttagattt
     1861 gctgaaggaa acgaaaaaag gattcttaca aaaaatgttc cctgaaaacg gagcaaaagt
     1921 gccagaaatc cggtttcctg gttttacgga agattgggaa gagcgtaagt tgggagaatt
     1981 aggtaaaact caatcgggaa ttggatttcc tgatgccgag caaggtggtt ctgaaggtac
     2041 tccatttttt aaagtttccg acatgaataa cattggtaat gagtatgaaa tgagaaatgc
     2101 aaatcattat gtatctaatg agcaaattga gcgtaaaaaa tggaagccaa taaaagatgt
     2161 gccagcgatt atatttgcta aagttggcgc agcaataatg cttaatagaa aacgtttggt
     2221 cacaagccct ttcttaatag ataacaatac catggcttat ttatttaata atacttggga
     2281 tatctatttt ggtaaaatat tgtttgaaac tataaatttg ccaagatact cccaagtagg
     2341 ggcattgcct tcttataaca gttctgatat tgagaatatc agtgttaagg ttcctgtaaa
     2401 ggatgaacaa caaaaaatcg gtactttctt caaacagcta gatgatacga tcgctcttca
     2461 tcaacgtaag ttagatttac taaaagaaac gaaaaaaagc ttcttacaaa agatgtttgt
     2521 ctaggaggga agaagaatga aaattagtca ccgtgaggaa gcagaagtcg aagaacagtt
     2581 gattcgtgta cttggagaag ggcataatca gtggacgtat cgtccggatc taaaatcaga
     2641 agaagacctt tgggtcaatt tacgccaaaa aattatctca aataatcaag cagaactgaa
     2701 tgattttcct ttgaccgata aagaatttga aacgatcaaa acggaattac tgctgcgaac
     2761 gaaaacccca tttgatgctg cgaaatggtt aaaaggtgaa aatgggatgg ctcggatcac
     2821 gattgaacgg gaagattcgc aattgggttc tgtctcactt atcctttatt ccaatcaaga
     2881 cattggtgga ggaatctcta cttatgaagt ggtccatcaa attgcaaaac gtggaagtaa
     2941 catagaagca cgggatcgac gttttgacgt caccttatta atcaatggat taccgattgt
     3001 ccaaatcgag ctgaaacaag taactgcaaa agatggtgtt tatcaggcat ttaaccaaat
     3061 caaaaaatat gcagaagaag gtatgtttcg gaacaatatc ttttcgacct tacagttgtt
     3121 tgtggtttcc aatgaacaaa ccactcggta ttttgcaaat gccttaccga aagacttgca
     3181 ccctaagttt ttatttagtt ggcgaacgaa agacaatgaa aaagtagaaa acttatatga
     3241 gttttgtaaa caagtgttaa atatcccgga tgctcatcgt ttgattgcgg attatacaat
     3301 cgtcagtgaa gatcaagaca ataaaaccct catggtgtta catccttatc aagtacatgc
     3361 gattcaagcc ttgtttattg ccgcaaataa acatcaatca gggtatgtgt ggcatgcgac
     3421 tggttcagga aagacgttga ctagttttgt atctactaag cttttagcac gaaaatcggg
     3481 gatcgatcga acgatcatgt tagtggaccg caaagattta gataatcaaa ccaccacaga
     3541 gtttacaaaa tttgcttcgg aatttaatac tgggatttct tccgggaacg ccaaagcgaa
     3601 tagcttgatc gttggaacgg gaagtgccaa agaattaagt gaaaccctgt tggcagatgc
     3661 caatgcaaat gttgtaatca ttacaacccg tcaaaaacta gatgcggcgt tgaaatatgc
     3721 aaagaaacaa gaagagaaaa aaggaaccaa tcgcttccaa aaattaatgg gccaacatat
     3781 cgtgtttgta gt
//
LOCUS       DAZUKZ010000172         3718 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000172 DAZUKZ010000000
VERSION     DAZUKZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.178"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..2543
                     /locus_tag="ACG8EF_002743"
     CDS             102..2543
                     /locus_tag="ACG8EF_002743"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFS4947989.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="ACG8EF_002744"
     CDS             complement(2873..3577)
                     /locus_tag="ACG8EF_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947990.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         1193 a          654 c          813 g         1058 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaa
//
LOCUS       DAZUKZ010000173         3713 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000173 DAZUKZ010000000
VERSION     DAZUKZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.179"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACG8EF_002745"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACG8EF_002745"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS4947991.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ACG8EF_002746"
     CDS             complement(2261..2512)
                     /locus_tag="ACG8EF_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFS4947992.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="ACG8EF_002747"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="ACG8EF_002747"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFS4947993.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="ACG8EF_002748"
     CDS             3462..3680
                     /locus_tag="ACG8EF_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947994.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          797 c          688 g         1203 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aag
//
LOCUS       DAZUKZ010000174         3691 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000174 DAZUKZ010000000
VERSION     DAZUKZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.180"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..545
                     /locus_tag="ACG8EF_002749"
     CDS             75..545
                     /locus_tag="ACG8EF_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFS4947995.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGFPYFDFGWHIFHAKFCDFNSIGKI"
     gene            complement(465..683)
                     /locus_tag="ACG8EF_002750"
     CDS             complement(465..683)
                     /locus_tag="ACG8EF_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947996.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            790..1104
                     /locus_tag="ACG8EF_002751"
     CDS             790..1104
                     /locus_tag="ACG8EF_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4947997.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="ACG8EF_002752"
     CDS             1208..2386
                     /locus_tag="ACG8EF_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4947998.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2567..2752)
                     /locus_tag="ACG8EF_002753"
     CDS             complement(2567..2752)
                     /locus_tag="ACG8EF_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4947999.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            2789..3082
                     /locus_tag="ACG8EF_002754"
     CDS             2789..3082
                     /locus_tag="ACG8EF_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFS4948000.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(3170..3316)
                     /locus_tag="ACG8EF_002755"
                     /pseudo
     CDS             complement(3170..3316)
                     /locus_tag="ACG8EF_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT         1259 a          546 c          716 g         1170 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat aatcccgtcc
     3661 taactctttt attcttccct catagcttcc a
//
LOCUS       DAZUKZ010000175         3487 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000175 DAZUKZ010000000
VERSION     DAZUKZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.181"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACG8EF_002756"
     CDS             complement(<1..2232)
                     /locus_tag="ACG8EF_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4948001.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACG8EF_002757"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACG8EF_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          982 a          772 c          548 g         1185 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacact tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaat
//
LOCUS       DAZUKZ010000176         3405 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000176 DAZUKZ010000000
VERSION     DAZUKZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.182"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..1195
                     /locus_tag="ACG8EF_002758"
     CDS             155..1195
                     /locus_tag="ACG8EF_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS4948002.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2019..2351
                     /gene="traE"
                     /locus_tag="ACG8EF_002759"
     CDS             2019..2351
                     /gene="traE"
                     /locus_tag="ACG8EF_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFS4948003.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2970..3311
                     /locus_tag="ACG8EF_002760"
     CDS             2970..3311
                     /locus_tag="ACG8EF_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFS4948004.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1266 a          460 c          646 g         1033 t
ORIGIN      
        1 aaggtagtac ataattcatt ttatttctga ttttaggtag tatttaatta aaaaacagtt
       61 ttaatgcgtt tgcatttttt atttatcttt gctattttcg aaaagagtag gtaattttac
      121 ataaataaaa caacgtgaaa gtagaggatc tacaatgact gattttaaat tttttaaggc
      181 ggatagagtt tacaacgaat tattttatca atttccaaaa gtctttattg tttctgacga
      241 atacaaaaaa atgaaagatt caactaagat tgcctatatg cttttaaaag caagattaga
      301 gatcgcaatc agcaaacggc aaatcgatga agaaggtaat gtttatttta cttatacgac
      361 aaatgaacta tgtagagtat taaactgcca aaaacaaaaa gcgatagcaa tcaaaaaaga
      421 gttggaatcc tttggtttat tattacaaaa gcagatggga tttaacaaac agttagggaa
      481 aaataatcct aatagactat atctagcaga attaaaagtc tcagaaaatg atatctactt
      541 actcgaaaaa tttgatagag agaataggga aaacgttgat aaatcagagg gtatgaaaat
      601 catacccacc ctcgacgaaa aatcagacgc tgaatccctt ggggctcaag agggtatgaa
      661 aatcataccg tgccaaaacg ttgataaatc agagggtatg aaaatcatac cagaacttaa
      721 taataatata ttagacacta atagacacaa tatagacact gaaaaagacc gcctacaaga
      781 tcaattgttg ttagacaatt ttgagacaat tatgacaaac gacagcattg ctacgtttgt
      841 ccctgaacga gtattaaatt tgataaaaac attttcttca agttacagtg aagctcaaaa
      901 aaccgtccag actattcata atgcaaagaa aaaagctgaa atagaaagtg atatttcgat
      961 agtttttgaa gaactcgata gttattatgt caatgcagaa caagaattat acacgacact
     1021 gttaaaagcc tatcaaaaat taaaaaccga aaaagtcgaa aatatccaga acctgatttt
     1081 tgtctatgta aaaaattggt ttatcgaaaa accaatagct gctaaagtat caagtgaaaa
     1141 acgtttgaat tatgcaagct ccccaagcac tattacgaaa gactggttag agtgaaactt
     1201 tactgacaaa gtgattgttg cttcatggag taacattaaa attaaaaatt ttttattatt
     1261 ctaattatat tacgaatatt acaaaataat agctatttta atagaattta taagttaaaa
     1321 tattatgata aaaatcacaa acgtagcctt ttacgtcata agtgttaaaa gaaataatta
     1381 atttcaaata tagaaaaggg aacgtatgtt tgcatactcc ttttttttga gttataataa
     1441 tacgcgaaga gagataattt cattatttaa acaagctaaa aatcttgata ttacttcttt
     1501 tcaggcaata cgaatattat tcgcttacat tttttgtata aatttaattg tctgctatgc
     1561 ttgtgatgaa ttgattttcg ttttgcactt ttggatgata aatttggaca attgaaaata
     1621 caaaaaaaag aaaggcggta tgaactcgga aggctggtaa ccttcgtcag cgttcataca
     1681 cggcattaag atttggccct taatgcgtta acctttcagt gttacggtat tacccgcaat
     1741 ggatttagta taacagatat tcaaaagtga taccactttt tttggctgtg ttaaggttaa
     1801 aaatacgtga tattacgatg aatctatgcc taagcataat gttataaaaa tgtccgtgtg
     1861 gctctttgcc gtatgacgaa cgctgaaatt agagggaacg catgtttgga tgcgttccct
     1921 cttttttgtt tgtcatgaac gaaaatcaat tctctatttt tgaaaaataa caagaaaaac
     1981 ttgttaaaaa tatttaaaga aattgggtgg tttattttat gtcatataca ggaattttga
     2041 gtctcaaaga tatttgtcac tacggtaaac ggtgtaccgc tacagagaaa atcacgaaga
     2101 aactatcaac tggccaaagt aaaaccgttg tgcagtgtaa gaaatatata atccagaaag
     2161 acaaagtctc agaggaaatg atttattaca ttgggaaaca aaagcaaata attttaaaag
     2221 atcctatccc cttaaaagag ctatatccaa caatcaaaca tgtttatgac caaaacggtg
     2281 tactgattgg tcgaagaaaa aatggagtct tgcgttgtac cgcaaaaggg atggggcgtt
     2341 tgatcggctg aattgatcat tctcataact tgtattttat cgtttttcgc ttgcttgaaa
     2401 atattgtcga gacttctaaa ggaacttaga gtcactagaa aaataaggag tagacttgat
     2461 ctatcaacaa ttcccttcgg ggagtgacgg ggtaggtttt aacgattggt aacgtaaaaa
     2521 attggtcaat cgagtaaatg cgcttgtttg gatagtggtg aaatgaattt gtgtaaataa
     2581 taagcgcaga gtaaacaaga gggaatttag aagttagaca agttagaata gcgtctagat
     2641 taatcatgag cagctactaa gggatctaga ctttgacgca aactcatgga ataaggagta
     2701 ggtcttctcg taatggggag gacgcacaag aaattgtgtc gtgtgctgca gtacatgtag
     2761 atacttgcga ggacgttgat tattggatca atcgagcatt taccgctgtc tactagggtt
     2821 tctcgtttcg gacgccataa acgagtcgaa attttttatg tatttttaag taaacgaaaa
     2881 acgataaaat atgaacaagt cctgaataaa gtgaaaaaat taatataaag catagagaaa
     2941 atgaaattta cgtaatggag gaatgacaaa tgaaaaatta tgatccgaat atacgattgg
     3001 gcacacatac tattaaggtt tctttccaac gttgggatta caaaggattt gtgacgttta
     3061 gaaggggcgg aaattgtaaa ggattagatg ttttagcatt agacgaggat gatttatacg
     3121 atcaaacatt gactgataat ccaattggat tcggcttgct acctgaagat gatgaaggta
     3181 atgaatggtt taaaatgacg cttatgaatg ataatggaga cgaattatct gttgaagata
     3241 cttggtcata tttgagtgat tatattgtca gctttgaaat cattgaattt gtagcggata
     3301 aagaagaata aaaactggag gaataaattt tacagataaa ggcttagaag gagtcacgac
     3361 gattaataaa aaggtagtat taccttaggc attgagtaag gaatt
//
LOCUS       DAZUKZ010000177         3330 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000177 DAZUKZ010000000
VERSION     DAZUKZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.183"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..196)
                     /locus_tag="ACG8EF_002761"
     CDS             complement(<1..196)
                     /locus_tag="ACG8EF_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4948005.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKY"
     gene            complement(527..760)
                     /locus_tag="ACG8EF_002762"
     CDS             complement(527..760)
                     /locus_tag="ACG8EF_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS4948006.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(775..1725)
                     /locus_tag="ACG8EF_002763"
     CDS             complement(775..1725)
                     /locus_tag="ACG8EF_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFS4948007.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1722..2564)
                     /locus_tag="ACG8EF_002764"
     CDS             complement(1722..2564)
                     /locus_tag="ACG8EF_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFS4948008.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(2566..3285)
                     /locus_tag="ACG8EF_002765"
     CDS             complement(2566..3285)
                     /locus_tag="ACG8EF_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4948009.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT         1095 a          630 c          401 g         1204 t
ORIGIN      
        1 ggtatttggt aaactcttca ttatacatat taataagcgc attaatatgc ggctgaataa
       61 tttcttcaaa taacgcttcc ttagaagtaa aatgtttata aaaagcaccc gtagttacgc
      121 ctgcggtcag gcacagttgc cttatcttag tttgctgaaa tccttctttt tcaaaatggc
      181 ttagtccact ttctattaac ttttggtgag ttataatata atctctggtt tctatgggta
      241 cttcctccta ataagatgaa ttaactttat tatcttacac aggacttgaa gtttcaatcg
      301 ttaacgttta ttactgaact cattccttag agctaaacta atgagaaaga gcagaataac
      361 tgatacaaaa agcaatccta caagaattaa tagactcatc taaatcattc accttttcta
      421 aaataatccg agaaagattt aaaaaaatta tatccagcta ttaggataag tcctatcata
      481 atcactccaa tagccatttt cttatcctcc ctaaaaataa agtcttttac ttaaaacctt
      541 ttttgtaatc gtcaataaat tttttccctt tatcagttaa ttgaaatcct atatctaaat
      601 agtcctcata tctaagttta gccagcatct cacttacaga tgctggacta acacttagaa
      661 cttttgctaa atcattattt tttaatttct tttttctttt tgataattcc tgaattgcta
      721 gtaaataaat tagtatagta gtcgttttaa tttgtaccat acctaccctc caaattattt
      781 cgaaaggcct tcagcaattt tatccaggtt ccatttcatc atcgcatagt aactatcacc
      841 atcttgtcct ttttctgcaa ttgaatcagt aaaaatcgtt gaatagatag gaatattggt
      901 atcttttgat actgttttca ttggtctatc atccacactg ctttctacga ataaggaggg
      961 aacttttgtt gtgcgtaatt tttcaactaa gtgttttatt tgatctggtg ttccttcttc
     1021 ttcagtgttg atttcccaaa tgtaagcaga aggcacatta tacgctttcg agaaatattt
     1081 aaagcatcct tcacttgtaa caatcatttt tttatcattc ggaattgaag caaatttaga
     1141 tttagcttct ttgtctagag aatccaattt ttcaatatac ttatctagat tttctttata
     1201 gaatttttta ttatcaggat ctttttccgc taactgtttt tcaatatttt tagcgtaaat
     1261 aataccgttt tctaaattta accaagcatg aggatcttcc ttccctttct cactctgacc
     1321 ctctaagtaa ataacatcta tgccatcact tgctgcaaaa tagtctttgt tttcctcttt
     1381 gttcgcattt ttaactaatt ttgtaaacca agcatttcct ccagtttcca agttaacacc
     1441 gttataaaaa atcaaatctg cttttgaagt tttttgaaca tcttcaggca aaggttcata
     1501 ttcgtgggga tctttcccaa taggtacgat actgtgtaaa tcaattttat cttttgctat
     1561 attttcggta atatctgcta aaatcgaatt agtaactact acttttaatt tttcgttcga
     1621 agccacttta tctttgtctt tactatttat tgaacatgcc ccgaatataa aaagagcaat
     1681 caaaatcccc agacttccca gtactgcttt ccattttctc atcataacga tctctccttc
     1741 tttcttaaaa atttttgttt tggtgagatt agaaaactaa ttacaaataa gaatgccgct
     1801 gttaaaacaa tacttgatcc tgctgctatg ttaaaactat aaccaataaa caatccaagc
     1861 aatgatgaac ttgctccgac tgttgcagaa ataaatatca tagttttcaa gctatttgca
     1921 tataaatatg ctgtagctgc tggtgtaatt aacattgcaa caattaatat agttcccaca
     1981 ctttgcatag ctgttactgc tactaaagtt aatagaacca tcaatagata atgataaaaa
     2041 ctgacattca tccctattgc ttttgccatt aatggatcaa aagaagtgat taataattct
     2101 ttaaaaaata gaactactat aaccaacact aaaaccgaaa cgccaattgt aatccactta
     2161 tcaatatctt gtacagctaa aatatttcca aataaaatat gaaataaatc cgttgagcta
     2221 tttgcaactc ctataaggat aactcctagt gctaaaaaag aactgaaagt aataccaatt
     2281 gctgtatctc cttttattac actattgctt tttataaaag taataatgat agaagctaat
     2341 agaccaaata caatcgcacc aataaaaaaa ttaataccta aaatataaga aattgctact
     2401 cctggcaaaa cagcatgaga aatagcatca cccataagag acattcctct taaaataata
     2461 aaacaaccta ctgttccaga aacaatccct ataactacag atgtaattag agcattctgt
     2521 aaaaagtgaa agtcatacag accattaata aaattttgaa gcatattatt ccccctctcc
     2581 aataaatata gtatctccat aggctttttt taaattttct tcattaaata cagattctac
     2641 acttccgtga gcaattagat ttcgattcaa aagaataata ttatcaaaat acttctttac
     2701 cttgcttaaa tcatgatgca caattagcac tgtttttccc tgatgtttca atttttttaa
     2761 cgtatccata attattcgtt cactaactaa gtctatccct acaaatggtt catctaaaaa
     2821 tatgaaatcc gcattttgag caagacatct tgctaaaagt acacgttgaa attgtcctcc
     2881 agataattct cctatttgat gattagaata ttttcccata tcgacctttt ccaaagcctt
     2941 tgaaactctt tgccattctg catttttaat tctttgaaaa actttcattc cggcatatgt
     3001 tcccatagat acacattctt ttacttttat tggaaaagta aaatcaatat cagctttttg
     3061 ctctacatat gcaatcttca ccagtttttt attgatatct tcattattaa tggagacctc
     3121 tccctgatga gggataatat ttaacattgc tttaagcaat gtggattttc cagcaccatt
     3181 tggaccaata atacctgtta aagcaccttc ttgtattttt aaagaaatct cgttcaatgc
     3241 taaaaaatcg ttataacaaa cattaagctt tttcacttct aacataactt tctccttcta
     3301 aatgataatg attatcacga tcatatggta
//
LOCUS       DAZUKZ010000178         3280 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000178 DAZUKZ010000000
VERSION     DAZUKZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.184"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..526
                     /locus_tag="ACG8EF_002766"
     CDS             <1..526
                     /locus_tag="ACG8EF_002766"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4948010.1"
                     /translation="VSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDLVVIRLELN
                     GKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVRSIITKKEN
                     RQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTVMEGGEDAF
                     TNRAKRYGFKQIVE"
     gene            576..965
                     /locus_tag="ACG8EF_002767"
     CDS             576..965
                     /locus_tag="ACG8EF_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4948011.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            952..>3280
                     /locus_tag="ACG8EF_002768"
     CDS             952..>3280
                     /locus_tag="ACG8EF_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4948012.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         1060 a          546 c          707 g          967 t
ORIGIN      
        1 agttagtggg gaaagtagta ttttttcaga taaaaaatta atcaattcta taagaaaatt
       61 gaatgatgca caagttcttg aattacaaac ccttaagcca aatgatttag tagttatccg
      121 acttgaacta aatggaaaaa aagttagttt atcgaatgct agtgctggtc aaaagacatc
      181 tgcaattctt tctatgattt tggcgtatgg aaattcacca ctcatattgg atcagcctga
      241 agatgatttg gatagtcaat taattaataa cttaatcgta agaagtatca ttacaaaaaa
      301 ggaaaacaga caaattttaa ttgtaactca taatgcaaat atcccagtta atggtgattc
      361 agaatggttg attagcatgg gagattcaaa agaaatatcg gctgatcagt gcgggtcggt
      421 agatgaaccc aatatcaagc caagaatatg tactgtgatg gaaggtggcg aggatgcttt
      481 tacaaataga gcgaaacgct atggttttaa acaaattgta gaataacact agagggtgag
      541 acatattatt atgtccgccc tttttaggag gaaaaatgaa aaaaataaaa agctatacca
      601 gtatctgggc agttgaaaaa gtcatttacg caatcaatga ttttcagttg ccttttcctg
      661 tgacttttaa ccaaatggct tggttcgttt tatctttact gtttgtcatc gtatttgccc
      721 atgtgcctcc actatctatg atagaggagg tctttttaaa atacttaggc ataccggtgg
      781 ctgtcacttg gtttatgtcg caaaagacgt ttgatggcaa gaaaccactg ggatttttac
      841 gatcctttat cagctatcac ttgcgattta aggtgacttt tgcaggaaag aaagtcaaag
      901 aacagaagaa acgatgggat gaacccagta ctcttgtaag gagtgtgaac tatgtatccg
      961 attaagtata ttgaaaataa tctcgtcttt aaccaagaag gcgagtgttt tgcctactat
     1021 gaattagtgc cttacaatta ctcctttttg tcacccgaac agaaatttca agtgcatgat
     1081 aactttcgcc aactaattgc gcaaaatcgt gaaggcaaga tccatgcgtt acaaattgcc
     1141 acagaaagta gtattcgcgc cacacaagaa cgatcaaaaa aagaaattac cggtagactc
     1201 aaagaagtag cgaaacaacg aattgatata cagacagatg cattagtatc catgattggc
     1261 gatagtcaga ttgattatcg cttttttatt ggctttaaac taattgcgac agatgaagaa
     1321 gtcaatctaa agagtctcaa gaaatctttc ttttctgggc ttcaagaatt tgtctatgga
     1381 gtgaatcatc atttgatggg cgattttgtt tccttatcca atgaagaaat tcgctgctat
     1441 tctaagcttg aaaaattaat ggaaagcaaa ttggctcgaa gatttaaagt caggcgagtc
     1501 acaccgagtg acctgacgta tttgattgaa catatttatg gcgaaaaagg gataccgttt
     1561 gaagaatacg aatttcagct accaaagaaa aagctaaaat cagaaacact ggtgaaacgt
     1621 tatgacttat tacgtccaaa tcgttgttta attgaagaaa aaccaaggtg cttacggatg
     1681 gaacatgaga atcatgagtc gtacgtagcg tatttaacaa ttaatacaat tgtgggggag
     1741 atggaatttc cttcctcaga acttttctat taccagcaac aacaatttac attcccaatc
     1801 gacacaagta tgaatgtaga gattgtgacg aataaaaagg ctttagctac cgttcgtaat
     1861 aagaaaaaag aactgaaaga tttagacaat catgcttacc aatctgacaa tgaaacgaat
     1921 tccaatgtct tagatgcttt ggattccgtg gatgaattgg aaaccacctt ggatcagtca
     1981 aaagaatcta tgtacaaact gagttatgtg gttcgagtga gtgccgaatc agtggatgaa
     2041 ctaaagcgac gctgtgatga agtgctcgat ttttatgatg ataccaatgt aaaactagtc
     2101 cggccctttg gcgatatgat ggggctacat gaagaattct taccctcaag taaacgatat
     2161 atgaacgact atattcagta tgttacgtct gattttttgg cggggcttgg ttttggggct
     2221 actcagatgc taggagaatt agaagggatt tactttggct acaatgtgga tacaggtcgt
     2281 aatgtttacc tgaaaccggc attggcttca caaggagtca agggttcagt aaccaatgcg
     2341 ctggccgccg ctttcctggg ttctcttggt ggaggtaaat cctttagtaa caatctctta
     2401 gtctactatg ctgttttgtt cggtggtcaa gctgtgattg tcgatccaaa aggggagcga
     2461 ggagggtgga aagaaacctt gccagaaatt gcggatgaaa tcagtatcct caacttgacg
     2521 agtaagtcag aaaatcaagg tctgcttgat ccttatgtga ttatgaagaa aaagaaagac
     2581 tcggaaagtt tagcaattga tattctgact tttttaacag gaatttcaag tcgagacggg
     2641 gagaagtttc ctgtgttacg taaagccatt cgaacggtga ctcaaagtga tcaacggggg
     2701 ttattacaag ttattaacga attgcgaaaa gacgggtcac caatcgcgga aaatatcgcc
     2761 gatcatatcg agtcctttac cgattatgat tttgctcatt tgcttttctc agacggctcg
     2821 attacccaat cgattagttt agataaacag ctaaatatca ttcaagtggc ggatttggtt
     2881 ttaccggatg cagaaacgac ttttgaagaa tataccacca tggaattgct tagtgtttcg
     2941 atgctaatcg tcatttccac ttttgccttg gactttatcc atagtaaccg agaaatcttc
     3001 aaaattgtcg atttggatga ggcttggagt tttcttcaag tggctcaagg aaaaacgtta
     3061 tcgaataagc ttgttcgtgc tggtcgggcc atgaatgctg gggtctattt cgtcactcaa
     3121 aatagcgacg atttattaga tgaacgttta aaaaataata tcgggttgaa gtttgctttt
     3181 cgcagtaccg atattcatga aatcaagaaa accttggaat tctttggttt ggatcaggag
     3241 gatcaaagca atcaaaaacg cctgcgagat ttggaaaacg
//
LOCUS       DAZUKZ010000179         3265 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000179 DAZUKZ010000000
VERSION     DAZUKZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.185"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..497
                     /locus_tag="ACG8EF_002769"
     CDS             <1..497
                     /locus_tag="ACG8EF_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFS4948013.1"
                     /translation="FNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKAL
                     DIETALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNE
                     NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFY
                     SSLI"
     gene            complement(583..1677)
                     /locus_tag="ACG8EF_002770"
     CDS             complement(583..1677)
                     /locus_tag="ACG8EF_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFS4948014.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1866..2627
                     /locus_tag="ACG8EF_002771"
     CDS             1866..2627
                     /locus_tag="ACG8EF_002771"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4948015.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2742..3182)
                     /gene="fabZ"
                     /locus_tag="ACG8EF_002772"
     CDS             complement(2742..3182)
                     /gene="fabZ"
                     /locus_tag="ACG8EF_002772"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS4948016.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT         1043 a          647 c          548 g         1027 t
ORIGIN      
        1 atttcaattc tgagtttggt caccttgaag gtgatacgat cgttggcatt catcataaaa
       61 gtgccgtcat tactttagtt gaaagattat ctaaagtcat tatcacgatt aaacccaacg
      121 gccgtaaggc attagatatt gaaactgccc ttaatcaatg gttttctcgc ttccctaaaa
      181 acttctttaa atctattacg tttgactgtg gaaaagaatt ttctaactgg aaagccatta
      241 gtaaccaaca tgatattgat atatattttg cggaccctgg aacaccttct caacgcccat
      301 taaacgagaa ttctaacggg attctgcgtc gtaatggact gccgaaatca atggatttta
      361 gaaaagtgaa tcagacattt atttccagtg tcagcaatca acgtaatcat attccaagaa
      421 aatcattgaa ttacagaaca ccaatcgaaa tatttttgag ctatgtacaa gaagcatttt
      481 attctagctt aatttgacaa atcataacct ataaaaaaac aaaataaaaa agcagaaata
      541 actggctatt tctagcggat tatttctgca ataactaatt atctattttt tattttcaat
      601 taatttaaag cttgaccatg gtttcaaaaa gtcaaggagg aataattcgt catcggagat
      661 tcttcctaca acattgacac gtccgtcgtt tttcatatcc ttcaaggcga tttgtgtttc
      721 tcctttgtat tgaccgtacc cttcattgtc aatcagtaca tccccatgat gcatatctcg
      781 cgtattatga ggcgggaaat ctttatcttt gtagtataca cgagtcatcg ttgaacgaag
      841 gatatattct gagcgatccc ctcgataact atgaaggtta tcaaacaagc agattcgttc
      901 gttttctgta ataccttctt ccgtatctac tttcaatgtt ggataatccg cgttaaatgc
      961 ttctgccatt tctttcaatt cttcttcact tgcgtacgca tttccaatag aaatatcatc
     1021 aatcaatcct gtaaggatta aatgcttcac ttgtgtcgca atttcaagat ctcggtggtc
     1081 ttccagcgtg cacaagccat cttgcgtagg ccaagggcca aactcagcag attgcgaatt
     1141 gacgaatgcc atcgtattca aattatattt tctgaatttt tctgaacaaa aaacaaagtg
     1201 ttcgtatcct aaaccagagt atcgatgagg ataaaaatta tgagaaccta ataaattatc
     1261 cgtgtttggc gaataactca tgatgttgtc aacgtaattc gtgcccgaac tcatattgat
     1321 ctcgattttg ataccaaaag gattgcgcgt catttttgct tcttctgctc ctgtgaatcc
     1381 gatatctaag cggaccccgt aggcacccat cttatggaaa aaagataagt catcgtaaga
     1441 aatctccaac tgttcaaata acgctggatt gatatcaacc attacttcca tgcctaggct
     1501 attcgcgtaa tcaactactt ctttaaattc tgaaagaact ttttctttat catcattgat
     1561 ctgcagcaaa ctggtaaaaa cccgtttata cccatactta tgagctaaat ccaagtacgc
     1621 tttgtccttt tcaaatgttg accgttctgg ataaatcgag attcctaatt ttcccatatt
     1681 accatttcct cctgcatctt ttcgtttttg tatccactta cattatagca aacacaaagt
     1741 aaatggtcta caccttttct ttttctttta aaataaatga taaattccaa taatttaagt
     1801 aggtattttc cctctatttt gttatcatta aagataacaa aatagtaaat tggaaaggaa
     1861 cagttatgaa gaaaaataaa gttattttaa gtattagttt agcagcagca ttgctgtttg
     1921 ctgctggttg ttctgcaaac aacacgactt cttctagttc gtctacagaa agttcaaaaa
     1981 gtacatctac tgcggaaagc acgactgcca gtagcgtcga tttgaacgca ttggaattac
     2041 cacaattgac gaaagatgtc gccgaaaatg aagatctggt cgaaatggtg acgaataaag
     2101 gcacaatcaa aatcaaatta tttcccgaac acgcaccaaa agcggttgaa aactttatga
     2161 cacatgcaaa agacggttac tacaacggac tgactttcca tcgcgtcatc aaagatttca
     2221 tgatccaagg tggcgatcct aaaggcgatg gcactggagg ggaaagcatt tggggagaag
     2281 gatttgaaac agagatcaac aaccaccttt ataatataag aggtgcgtta tctatggctc
     2341 gctcacaaga tcctaacagt aacggcagcc agttctttat cgttcaaaat acagatgata
     2401 tgcatgatgg tcttttaaaa gacgactatc ctcaagcaat catcgacgct tataaaaacg
     2461 gcggttatcc ttcactagat ggccattata ctgtcttcgg ccaagtgatt gaaggaatgg
     2521 atgtcgtgga tgctattgca aatatcgaaa cagatagttc agacaaacca aaagaagaag
     2581 taaaaatcga taaaatcaat atactgcaag aagcaaaaca aaaataaaaa aataacgtct
     2641 gaatcctgca tagatgacaa cgtatgctag gattcaggcg tcttcttttt acataaaaag
     2701 caagtaagct tccttttgtt ttgctatatc aggttttata gtcaagcatt ttggttgacg
     2761 atgaacgtga actcgcactc acagactttt ttatcatcta catatgctgc agataacgct
     2821 gtaccgactg atcccttcat tttggtgatt tcaaaatgac atttcagcac atctcctgga
     2881 acaacttttt gacggaattt ggccttattg atcccaccga tataagctct ttttcctaga
     2941 aactcttctg attttaggat aagtatcgaa cctgcttgtg ccagtgtttc tagaatcaac
     3001 gcacccggca tcactggatt gtcaggaaag tgaccttgga aaaaatcttc attgattgtt
     3061 acattttttg tcgcaacgat cttttttcct ggttctaaag tatctacata atcaatataa
     3121 caaataggat aacgattagg aatcaattcc attacttcag tagcagataa tacgtaagac
     3181 ataattaaac tccttctttc aggcaattgc cattttcatt actttgacta tcaaagtatc
     3241 atattcctaa atatttagca atctt
//
LOCUS       DAZUKZ010000180         3244 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000180 DAZUKZ010000000
VERSION     DAZUKZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.186"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..352)
                     /locus_tag="ACG8EF_002773"
                     /pseudo
     CDS             complement(1..352)
                     /locus_tag="ACG8EF_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(612..1160)
                     /locus_tag="ACG8EF_002774"
     CDS             complement(612..1160)
                     /locus_tag="ACG8EF_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948017.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1161..2018)
                     /locus_tag="ACG8EF_002775"
     CDS             complement(1161..2018)
                     /locus_tag="ACG8EF_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS4948018.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2304..2590)
                     /locus_tag="ACG8EF_002776"
                     /pseudo
     CDS             complement(2304..2590)
                     /locus_tag="ACG8EF_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2580..2909)
                     /locus_tag="ACG8EF_002777"
     CDS             complement(2580..2909)
                     /locus_tag="ACG8EF_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4948019.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2986..3066)
                     /locus_tag="ACG8EF_002778"
                     /pseudo
     CDS             complement(2986..3066)
                     /locus_tag="ACG8EF_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            3131..>3244
                     /locus_tag="ACG8EF_002779"
     CDS             3131..>3244
                     /locus_tag="ACG8EF_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS4948020.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDR"
BASE COUNT          962 a          646 c          421 g         1215 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac ttatttgaga gtcatctaat tggctcataa
      241 tcctttcttt ttggttagta tctgcagtta cttgtttagt atgagccatg aacccaaaca
      301 tactcacccc aacaaccaat cctaaatata ttttcttttt aaactttttc atctacatct
      361 cctcctaata gttttatttt agagttagct ctattactta tttactaact caacaacaga
      421 ttatcataca cttgttaatg aaatcacaat atctcattaa aacacaccct aaaatcataa
      481 ccgttcctat gctcattttt tggatttttt aatcacgtaa ccagaaaaat aaatcccctc
      541 tatttcacgc aaatcaaaga agaactaagt aactaccgtt taaaggtagt tacttagttc
      601 ttctttgatt attatctaac tttattattc attccccacc ctttgcgcag tgagtcttcg
      661 gtatcttgtt ttatctgact ggtagttctt attatttctc cacgatcatc taattctgga
      721 acaattggat atttttctct gaacaagtct agttctttca ccacgtcatt aaacaaatct
      781 tgcttatctt cttgcgtaag attctgttta atatactcat ctaaagcttt tctaagcaat
      841 tcatctcttg tcatattagt ctcatacttt tctactaaaa ctctggaagt cgcatttaac
      901 aaagtcgaca gttttaaagg atgcttggtg gaataaacct tatcaaactt tctgattggt
      961 gcgccaactt tttttgttct tcctctactc tgtctttcaa cattggagga agtttttatt
     1021 tcttgaactt ggtgcacttc taattgctta ttttcagatg gtgtaattgc ttgctgcttt
     1081 ttctttgctt ctgcttcttt ttttattttg gcgagtcctt tgttgttccc aaaaatatca
     1141 agtcctttga tgtcagccaa ttactccacc tccgcatacc aattctcgtg ttcttccaat
     1201 tctttcaaaa tactgataaa taattcgtgg cttttgcgat cccattgttt taagagacct
     1261 ttctttgtct tctccatcgt aatacctgat ccgtcgtatc gttttaagcg cgcatttttg
     1321 agtacaacat ttttaatcaa atgttcccca taaatctctt gggcttgttt atacatttcc
     1381 tgatctactg cgtcaccgct ttccaacatg aaaggaataa caccagcaac atataaattt
     1441 gtatcaaatt ctttcacaaa aaagtccata tactcaagat attgcatcgc atttctcatc
     1501 gattttactt gggtctgtaa gacgacgatt gcccaatccg caatgtacat cgcggattta
     1561 ctatatacag aaatagaagg tgggacatct aaatagatga cgtcaaactc atcaaataat
     1621 ggttctagct tttctttcat atatttgaat tgggataatg aatcattata ttggtctttg
     1681 atccaatcat catacatttc aaaggacatg tctgctggaa gtatatagag gttattgatt
     1741 actttataga cagcctcttt aggtggaaga ttgtttatca acacgtcagt cacagtattt
     1801 tcgtaaacaa tcggttcacc ttctccgcct ccttcttcgt tagtgaagtt catatgcgtt
     1861 aagttcatca catcagaagt atttccttgc atatccaagt caattatcaa ggttcttttt
     1921 ccttcattaa ctgctgattc aaaccctaac atttgcgcgc acgtactttt acctacgcca
     1981 cctttaaaat taccgattac atatttcttc gctaccacgt tcctacctct tttcttttag
     2041 caatattcat ttgatactat tataacatat ttatttttag ttactaccaa aaaaaagata
     2101 tatatgtact accttataat taataagtaa aaggtagtac ataaataagg aaaagctgaa
     2161 atatattgat ttatagcttt cctcaaagta atgacgttat ttaatttatc gagaagcaga
     2221 gaatactcgc gactttagtc atgtagacga atcagatttt cgataaaaaa gaagccgtcc
     2281 ttcttaagaa cgacaacaaa tcactagatt aattttttca actgttcata aaagttttca
     2341 tgcgttccac ataacaatac tggaataacc ttgttttcta taatctcgta agctactcta
     2401 tatgttgtgc ccgcatattt gaacccacta gaccaaatac ctgataaatc cccagttttt
     2461 tgttcaccct tatatggatc aatcgcaatt tcatcataaa ttaaattcaa aaatttttct
     2521 ttcaatggct tctcttttag ctttttaaat acttaatgac atgttttgaa ggaacgattt
     2581 cataagccaa tttctgttgc caactcctct ttagtcatta ctttagaatt agtaagtgta
     2641 tcttcagcaa tatctctaaa ggaagaatgc attttgtttt ttctattctt aaattctaca
     2701 agaatctcat ctttatcgta gccttctgca attatatcag acaaaatatc ttcgctaaaa
     2761 tcataaaatt ctttttgtgg ttcaacaaat tcataaagaa taccattctc taccaacttt
     2821 gcttcaactt cgactccatt aggaacatta aaatctttag gtactgtcaa catgattgaa
     2881 ttgccttgtt ttcttgtttt tgtaatcatt taaatccctc atttctaaca gtatgtaatt
     2941 acttagtaaa tacattatac cacgagataa tgtatttaca agtaattact gtgactcgta
     3001 atgcgtcctt gctgaataaa tttcaaccac gtgttcatct tcatctacct tataaacggt
     3061 tctgttgcaa agttttccaa aaaatctatt ttagtgtaaa attgagaaaa aagacagaga
     3121 ggacagagta atgaatcatt ttaaaggcaa acaattcaaa aaagacgtca ttattgtcgc
     3181 tgttggttac tacctgcgtt acaatctaag ctatcgtgaa gttcaggaat tgttatatga
     3241 tcgt
//
LOCUS       DAZUKZ010000181         3221 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000181 DAZUKZ010000000
VERSION     DAZUKZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.187"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG8EF_002780"
     CDS             301..585
                     /locus_tag="ACG8EF_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948021.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG8EF_002781"
     CDS             588..1004
                     /locus_tag="ACG8EF_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948022.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG8EF_002782"
     CDS             complement(1141..1860)
                     /locus_tag="ACG8EF_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4948023.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2544..2630
                     /locus_tag="ACG8EF_002783"
     CDS             2544..2630
                     /locus_tag="ACG8EF_002783"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS4948024.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..>3221)
                     /locus_tag="ACG8EF_002784"
     CDS             complement(2932..>3221)
                     /locus_tag="ACG8EF_002784"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948025.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DAZUKZ010000182         3213 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000182 DAZUKZ010000000
VERSION     DAZUKZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.188"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..443)
                     /locus_tag="ACG8EF_002785"
     CDS             complement(<1..443)
                     /locus_tag="ACG8EF_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4948026.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNIS"
     gene            513..863
                     /locus_tag="ACG8EF_002786"
     CDS             513..863
                     /locus_tag="ACG8EF_002786"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4948027.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            1277..2095
                     /locus_tag="ACG8EF_002787"
     CDS             1277..2095
                     /locus_tag="ACG8EF_002787"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4948028.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(2168..2527)
                     /locus_tag="ACG8EF_002788"
     CDS             complement(2168..2527)
                     /locus_tag="ACG8EF_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4948029.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT         1059 a          475 c          574 g         1105 t
ORIGIN      
        1 tcagaaatat ttcttttgaa tgcctgtttt ccacgttttt ccttataacc atttggtttt
       61 cgttttcctt gcatcggtaa atgtaaaaca ttgaattctc ccgttttaaa ccggcgatag
      121 agggtactta ctgaacaaga gaaggttcgc tctgcacggc caataatgac gtctggggtc
      181 caaccttcag ttgatctttt tgcaatgtat tctttttcct cagcaggaaa aatgatttcg
      241 gttctcccac aacgtcgctt attttctttg tattgttcaa agtattcaag tgctgttcca
      301 ccacatttga gaaagttata gacattgtaa attgtttggc gtccacgttt aagctgcttc
      361 gcaacgatgg caaccggagt ttcttgatga aaatatgctt ctatcattac aagctcgttt
      421 ggtgtaagat gggtataagt catttatgtt cactctcctt gtatgcttta gcgggtatta
      481 caatttgagt gtaacataaa tggctttttt atttgtctcg cttaattata caaacggcgt
      541 tttataaagc aatcattcct gagaacttag atgaggttaa actttattta tgggaagata
      601 acggagaaat tgtcggtttt agtggtgtaa gtgcagaaga attagttatg ctatttcttg
      661 atccagattt tattggtaag cgatatggta gtcaaatatt aattgagctg attgaaactg
      721 aagatataaa aagaattgat gttaatagtc aaaataaata tgcaaaacat ttttatttaa
      781 gccatggatt tgaaattgaa tctgaagaag acttagatgg ttttggtaaa ccatacccta
      841 taacgcattt agtaaaaaaa taattcatga atggtttatt acctgattga acatgaataa
      901 aagtaatatt tttattttga tggcctattt ctaggttatg aattatccag atcaaatttg
      961 tgtattacat tcagtaataa ggtgcttatc ttaggatcaa cttatgatac tagctgacca
     1021 aggatgtacc agttatagaa aatcagttaa agtaaaaatg ataaaaatct tatgtcaaca
     1081 ctgatttagg ctgacctgtg ggttaggaga aagacaggtc ggtagctttt aatacaaaaa
     1141 atggcttgta tgtatctaga tagttaactg aaattagaaa tgttcttatt tttaacggag
     1201 taagaaaaat aggctgcttt aggtctgaat actttttcaa tataatgaag atatcaaagg
     1261 cgacaggagt gtttttatga ataagaatcc tcataatcta aagttggtag cagtagatat
     1321 ggatggaaca tttgctcgta ctgattacac atacgatatc ccaaggttca aggctgtttt
     1381 atcgcgaatg aaagctgttg attgtcaatt tgttgttgca agtggaaatc aatactacca
     1441 attgagatcg ttatttccaa attattatga tgaattaagt tttgttgctg aaaatggtgc
     1501 ttttgttaaa gataaaacag aggtactatt ttcagccgat atgcctagaa aagcgatcga
     1561 ggtggtcctt gatttatgta aagactatcc tgaagtaaaa aatgtgatgt gtggtctgaa
     1621 tagtgcctac tgtgaaagag gaaaggtgag tcaagcattt tttgatctaa ccaatatcta
     1681 ttaccataga ttagaatggg tagctgattt tgcagaagtt caagataaaa ttttaaaatt
     1741 tgctccgact atgcctgttg aaaaaaccga attttacgtt gaattgttcc gtgaaaaatt
     1801 agccgggcta ttagtaccta ccagtagtgg tcatggttct gtagacttga ttctttctga
     1861 gtgccataaa actttcggct tgaaaatgtt agttgagcgc ttggggatta atcctgacca
     1921 gtgcgttgct tttggagatg ggggcaatga cattgagatg ttggagtatt gtggtctgag
     1981 ttatgaaatg gataatgcaa cggaagctgt taagcaagtt gctaagcatc aatgtccttc
     2041 aaatgacgaa gacggtgttt tagtgacgct cgatcgattg ttccctaaca cgtaaacact
     2101 aggtaattca tcaaaaaacc tcgatataag aaatattcga ggttttaaaa ctatatttag
     2161 aacagattta cgaagcgtct gatgagtcaa aatagacttg gacattgcta tctaaaatac
     2221 cactttcatt ctttaaacga attgtttgat ttgatggagt tacctctaat actaaatgcc
     2281 cattaataaa ggtagtatcg tattcagatg ttttaactgt tgcttcttcg caaggtattg
     2341 ttaaatcata tgctggaata taatgttgtg tggaaactaa taaaaaatga tcaccaatca
     2401 tacagcatcc ctctgctgaa gaaaattgat tagagccatc atttaaagtt aacaagaatc
     2461 catcctttaa acgaggtact tgcttaaata cttctggttg tacttctaaa aataaattat
     2521 cgctcatttt cacgaccacc atcgacttcc acagttgaaa ttgacatttt atttaacaca
     2581 cctattcccc cttaactata actcaattct atataaaaaa tattctattc tcaaataatt
     2641 gaacttttca aatcgccaat ttacttcggc tttatcagag ctcattcata tcaagagcga
     2701 ttaacgctgt aaaaattaaa ccgaagatct gataaatttt ctctattttc tacaatattt
     2761 aaaacgcttc cttagtctat tatatatcta tttctcttag gagtatgttt agatgtttga
     2821 ataatagttg cccaacttta aaggttattc ttataatatt ttacttcatt ttccaataga
     2881 aaattcaaag gattactggt ttttctaaaa tagtttagtt taaatacata agtatcctcc
     2941 gacactgtat tagttcataa tcatctaaat acaaaatttt caaataaaaa tatttcaagc
     3001 tctatcgttg acgaatgtaa acgatactta taaagtaata gaaattgatt acatttgtaa
     3061 acttaaaatg gttaatttat ctcacataaa actaagaata aaataatgat taaaagtggt
     3121 tagtttttat acgatttagc ctatcggtaa aaatgaatta gagagtgtaa aatattttgt
     3181 gtaaatgaaa aaatccatac aaaaaaggaa gtc
//
LOCUS       DAZUKZ010000183         3165 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000183 DAZUKZ010000000
VERSION     DAZUKZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.189"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(385..999)
                     /locus_tag="ACG8EF_002789"
     CDS             complement(385..999)
                     /locus_tag="ACG8EF_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4948030.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1605..1982)
                     /locus_tag="ACG8EF_002790"
     CDS             complement(1605..1982)
                     /locus_tag="ACG8EF_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948031.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2282..3163)
                     /gene="galU"
                     /locus_tag="ACG8EF_002791"
     CDS             complement(2282..3163)
                     /gene="galU"
                     /locus_tag="ACG8EF_002791"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS4948032.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          964 a          655 c          489 g         1057 t
ORIGIN      
        1 atcaggtaaa cttcattgca ataatgataa caaatgtact ataattactt tgtaaagagt
       61 tttggggaag actctttcat caacgtgtat taagagacaa gaaagaatcc atatggattc
      121 tttcttgtct ctttttccac tactttttat ttttctgaca ctacatattc ttataactca
      181 tttctcataa ctgtgaaaca acttataaaa ataacaaact ttaaacaaaa caagcttaaa
      241 atcaaagatg atccatctta aaataactaa aacaaaaaaa gtagtcgttg ttaatcctca
      301 ttaataatcg ttttcttttt ttcaaacgct ccatttttat gtggaacatt atttttttaa
      361 ggaactttgt caacatgtgg ataactattt tccacttgtg tcctggacta gctgttttgc
      421 tgtaaaatac aaaacttgct gcagcaaaaa gatcgttgca agagaggctg ttccgcagat
      481 cgctggatct agatttggca aagtcaagac tgcatcatgt aaattggcgt ggttgtccaa
      541 aaccaaatca gcaaattcaa ataatcgcaa accactgcta tgttgcgatt tgatacttcg
      601 tgacaaatca aatccagtaa caatgattaa tggatgacca ttttctttga tccactgagc
      661 tagttcgatg attgccgggt ttcttccttc attcgaaaga agaagaaaaa tgtcttctga
      721 tttgacagaa agctgatcaa taattgcctg tcctgcacct tctagcgttt catagacacc
      781 tccggctgga ttttcaatca atatacttgg tacaagatcc ggggtttgat cccaaaattc
      841 aaatgctaca ccacttagtg tgcggctagc atatatctgc aatctaccac cttgattaat
      901 ttgtttcgcg atccaagtgc cagcttgaag catcagcggc tcttctatcc ttaatttctc
      961 ttccaattgg tgataatagt caaaataaca taaactcatt tatctctccc gcctagctat
     1021 acttgacatg aatagaaaca gctgcctaat ctaaggaagt acataaaaaa acagtagtta
     1081 gacagtaaca catgtggtct ttgctgtttc aaacacaaaa aaggcattga acgaatacgc
     1141 ccaatacctt gacatagcaa ataaaacaac gtgcagacca tggaaaatat ctacataggg
     1201 gtagaatttt caccctagat taggtacaat aaattaggtc gatgcactgc attgactatg
     1261 gcaaaatgat cggtcagggt gtatgtgtgt gactggtcat tccctgattc ttagcgccaa
     1321 ctaagggtca gggagccttt ttttattcag ctgtttctat ctgaatttat tgtaagtaat
     1381 ctcttatcca agtgcaagca agaaaataca aaaaaacatt ggtatgattt atatatatat
     1441 aaataacata aaaaaaggaa tggcttatcc cgatacaaca agcattcctt taaaaaagat
     1501 tcttttatat aagtgaaaaa agcttttttc caccaattaa ataaatctct catctcaata
     1561 gtttatccat tattttatct ttttttgcgt atacctacta aatattaccg tcttcttctc
     1621 gttcttctac tttgttcgag agaatctgta ctagaaaaag tagttgtatg tctacctttt
     1681 ctgcctatcc gagactttct tttcatatat gggatataaa tgattagata cagtacagca
     1741 aaaaataaag caaatacgca agcttcccaa ctacctaacg ccaattcaac catcaaggta
     1801 gcaaccccaa cggatgctgc ataccagata aaaatcagaa acgtttcttt ttttctgacc
     1861 atgcgaaaca tgataaatag actaataagc aatagtggca acacaaatat ccaattgctt
     1921 ccttccatca gccacttgag tatatcaagc agcgactgtg caacagcgac taattttccc
     1981 atagtgaaca cacacctttt tttataatta aaaaattcgc ctttttattc tactatgaat
     2041 cttctggctt ggctttcatt tctcttatgt aatcattttc ttttatacaa cttaaaaaaa
     2101 aattaaacca ttagcataat attttctgaa tctattcacg aaagtctttt tctatctttc
     2161 gttttccacg actcaactga tctagagtca aaaaactaaa acgacagttg cgcaaatgac
     2221 gcacaagctg ccgctttaat aacaaatagc ctttcattct cagcaggcca cttatttcca
     2281 gttaactttc tgttcttagc tttttactca actggataat atatgcactt aatgaagtcc
     2341 caatttccga atgttgaagt ccatattcaa tatttgtctt catatatcct aacttatcgc
     2401 ctacgtcgta tctttttcct ttaaactctc tcgcatagac cgcctctgtc tggttaagag
     2461 tctgaagtgc atccgttagc tgtatctcgt ttcctgctcc tggttcttga gtttctaata
     2521 tctcaaatat tttaggagtc agtagataac gtccgataat ggctaaatta cttggtgctt
     2581 ttcccatttc aggtttttct acaaaccctt ttactctgca acgacccttt tcgctatctg
     2641 ttttaggatc aataattccg tatttagatg tttcattttc tggtacacgc atgacggcaa
     2701 cagcggaagc ttgtgtttct tcataatcca tcatcaattg ttgagtcaat gggatatcat
     2761 ctttcatcaa atcatccccc agcatgacga caaacggttc atttcctata aatgcttttg
     2821 cttgaagcac ggcatccccc agtcctcttg gatgagattg acggatgaag tgaagattga
     2881 cttgagtcgt ttcttcaact aaagcgagta attctgtttt ttccttttcc cgcaaattgt
     2941 tttccaattc gatatttgca tcaaagtgat cttcaatggg tcttttttct tttcccgtta
     3001 cgatcaagat gtcttcgatt cccgaatcca atgcttcttc cacaataaac tggatcgttg
     3061 gtttatccac gattggaagc atttcttttg ccatggcttt tgttgccggc aaaaaacgtg
     3121 ttcctagccc agcggcagga atgactgctt ttttgacctt cactt
//
LOCUS       DAZUKZ010000184         3143 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000184 DAZUKZ010000000
VERSION     DAZUKZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.190"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..973
                     /locus_tag="ACG8EF_002792"
     CDS             191..973
                     /locus_tag="ACG8EF_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFS4948033.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(1099..1830)
                     /gene="erm(A)"
                     /locus_tag="ACG8EF_002793"
     CDS             complement(1099..1830)
                     /gene="erm(A)"
                     /locus_tag="ACG8EF_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFS4948034.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(1888..1947)
                     /locus_tag="ACG8EF_002794"
     CDS             complement(1888..1947)
                     /locus_tag="ACG8EF_002794"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFS4948035.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            2339..3001
                     /locus_tag="ACG8EF_002795"
     CDS             2339..3001
                     /locus_tag="ACG8EF_002795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4948036.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
BASE COUNT         1046 a          452 c          580 g         1065 t
ORIGIN      
        1 tcaaaaatta tatggagatc tgtataataa agaataatta ttaatctgta gacaaattgt
       61 gaaaggatgt acttaaacgc taacggtcag ctttattgaa cagtcattta ggtatatgtc
      121 caatctaggg taagtaaatt gagtatcaat ataaacttta tatgaacata atcagttacg
      181 aggtgaaatc atgagcaatt tgattaacgg aaaaatacca aatcaagcga ttcaaacatt
      241 aaaaatcgta aaagatttat ttggaagttc aatagttgga gtatatctat ttggttcagc
      301 agtaaatggt ggtttacgca ttaacagcga tgtagatgtt ctagtcgtcg tgaatcatag
      361 tttacctcaa ttaactcgaa aaaaactaac agaaagacta atgactatat caggaaagat
      421 tggaaatacg gattctgtta gaccacttga agttacggtt ataaatagga gtgaagttgt
      481 cccttggcaa tatcctccaa aaagagaatt tatatacggt gagtggctca ggggtgaatt
      541 tgagaatgga caaattcagg aaccaagcta tgatcctgat ttggctattg ttttagcaca
      601 agcaagaaag aatagtattt ctctatttgg tcctgattct tcaagtatac ttgtctccgt
      661 acctttgaca gatattcgaa gagcaattaa ggattctttg ccagaactaa ttgaggggat
      721 aaaaggtgat gagcgtaatg taattttaac cctagctcga atgtggcaaa cagtgactac
      781 tggtgaaatt acctcgaaag atgtcgctgc agaatgggct atacctcttt tacctaaaga
      841 gcatgtaact ttactggata tagctagaaa aggctatcgg ggagagtgtg atgataagtg
      901 ggaaggacta tattcaaagg tgaaagcact cgttaagtat atgaaaaatt ctatagaaac
      961 ttctctcaat taggctaatt ttattgcaat aacaggtgct tacttttaaa actactgatt
     1021 tattgataaa tattgaacaa tttttgggaa gaataaagcg tcctcttgtg aaattagaga
     1081 acgctttatt actttaattt agtgaaacaa tttgtaacta ttgaaaatag aaagaaattg
     1141 ttccttcgat agtttattaa tattagtgac atttgcatgc ttcaaagcct gtcggaattg
     1201 gtttttagtg aaaagaacac gatattcacg gtttacccac ttataaacaa aagatcgata
     1261 ctttttgtag tccttctttg aaatcaatgg ttgatgtcgt tcaagaacaa tcaatacaga
     1321 gtctacactt ggcttaggat gaaaatatag tggtggtact tttttgagca tttttatatc
     1381 catctccacc attaatagta aacccaaagc tcgttgcaga ttttgcaatc ttttcgcaaa
     1441 tcccttctca acgataagat agctatattt agcctgactt tcaaaggtaa ttcttttgac
     1501 aatatccgta ctgatgttat aaggaatatt accatatatc ttatagttta tatgttttgg
     1561 gaaggaaaat tttagaatat ccgtttgaat cacttttata ttctcagagg ggtttaccgc
     1621 ttctttagtc acttgacata agcctccatc aatttctata gcagtaactg atcgactcat
     1681 tttgactagc tctttggtaa aatgtccttt tcctgatccg atttctatta cgttgtcttg
     1741 tttactgata ttcgtgtgat tcaatatttc ttttacatgc tttttagaag taataaaatt
     1801 ttgcgtgtct ttagggtttt tctggttcat tataaccttc tccttactgg ttataatgaa
     1861 ctgacattaa gcgttcatta taaccaatta attttgattt ggttgataac gaactttatt
     1921 aataacaaat atagaaaaag tacccatact tttatctcct ttccattttt ttcattatga
     1981 atgagataaa gtaatttcta ctactgcgat actggtgcac ataattgaaa cctccttatg
     2041 taactgaata tgattgtatc atcttttcat tttagaataa acctttatat gtaattttgc
     2101 acttgacact tcagatggac gttttcttca aaataatatg ttttgaaact tgataagaga
     2161 agccactgtc tttactaaaa agtaaaggct gcgataaagg acagtgactt taataaagtt
     2221 gcactgttgt attgtctttg gataaaattg tctaaaggga agttttatcc ccaacgaaaa
     2281 aacatctaaa gtggtaagta atgaaagaat gtcgataaag taggaatggg ggatcttttt
     2341 gaatacaata gaatcacagt ttgataaggt tgcagaagat tacgatttcg tgaatgagct
     2401 tttgaatgat tattcattct ttgtgtctaa tatgtctcca aagaaaggca gagcattaga
     2461 tatcggatgt ggctcgggtt tgttagtgga gaaattagca agttattatg atgaagtggt
     2521 agggattgat atttctaatc aaatgctcga tcttgccaaa tctaaacgtc aactaacaaa
     2581 tacggtctat ctgaatatga atgcagaaca acttaatttt aatgagaagt ttgattttat
     2641 tgtaagccga acaacctttc atcatttgga tgatatagcc agcgtaatac agcaaatgaa
     2701 ggaactgttg aatgaggaag gaagaatagt tattcttgat aatgtatctg aagtcgagac
     2761 accgcctacc tatgtttata aattaggggc aatccaagag tttttaccgc attgtttcaa
     2821 atttggaata aaaaatgcga ttagaattta taatcacaac acatctaaat catggcttga
     2881 gcatttagct tcagataagt acctatctga acaaaattat tacgatttat atgaaaagtt
     2941 gttgcctgga tgccaattcc ataaaatggg ttgggcaatg ggggttgtct ggacaaaata
     3001 acttaaagct gtggctttga aaagttgaat ttgtgattgt tttcagagct atcgggtgct
     3061 ttagtataaa taacaatgaa taaaaaatca gcctgtatct tatgttacta ttaagtaatt
     3121 taagatacag gttttactat ttt
//
LOCUS       DAZUKZ010000185         3129 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000185 DAZUKZ010000000
VERSION     DAZUKZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.191"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..1347
                     /locus_tag="ACG8EF_002796"
                     /pseudo
     CDS             103..1347
                     /locus_tag="ACG8EF_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="ACG8EF_002797"
     CDS             1360..1992
                     /locus_tag="ACG8EF_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4948037.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2200..2424
                     /locus_tag="ACG8EF_002798"
     CDS             2200..2424
                     /locus_tag="ACG8EF_002798"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFS4948038.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2526..2759
                     /gene="relB"
                     /locus_tag="ACG8EF_002799"
     CDS             2526..2759
                     /gene="relB"
                     /locus_tag="ACG8EF_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS4948039.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="ACG8EF_002800"
     CDS             2749..3018
                     /locus_tag="ACG8EF_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4948040.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1102 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttta
//
LOCUS       DAZUKZ010000186         2971 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000186 DAZUKZ010000000
VERSION     DAZUKZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.192"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..690
                     /locus_tag="ACG8EF_002801"
     CDS             190..690
                     /locus_tag="ACG8EF_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS4948041.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="ACG8EF_002802"
     CDS             787..2634
                     /locus_tag="ACG8EF_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFS4948042.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..>2971
                     /locus_tag="ACG8EF_002803"
     CDS             2637..>2971
                     /locus_tag="ACG8EF_002803"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948043.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSIT"
BASE COUNT         1041 a          384 c          637 g          909 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta c
//
LOCUS       DAZUKZ010000187         2931 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000187 DAZUKZ010000000
VERSION     DAZUKZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.193"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1377
                     /locus_tag="ACG8EF_002804"
     CDS             <1..1377
                     /locus_tag="ACG8EF_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948044.1"
                     /translation="ILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEA
                     IALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLF
                     LSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSI
                     SDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFP
                     WFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVT
                     SIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVK
                     VGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLV
                     NVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKE
                     INKKNENK"
     gene            1364..>2931
                     /locus_tag="ACG8EF_002805"
     CDS             1364..>2931
                     /locus_tag="ACG8EF_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFS4948045.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTI"
BASE COUNT         1000 a          436 c          503 g          992 t
ORIGIN      
        1 attctgatta gtttttatta tggagaaaag ttttttaaaa agatagatgc tgctttttta
       61 tttagttgtt tatatacttt tgcaacttat cgaacagttg atatctatta tcgatccgca
      121 attgcagaag caatcgctct tacgattatc ccagctgttt tatattatgc ttatggattg
      181 atattcaata gagaaaaaaa agtcatcgct ttatctgtca gtataagtct acttgtgtat
      241 actcatgtct taactacact attgtgtagt gcattgattg cttgtatgat tatagttcgt
      301 ttgtttttaa gcaagcaaat aaaaaacgat ataaaatttg tggtcgtgaa tttagtaaag
      361 gctgtttttt tgacttgtgc attaactagc tatttttggt atcctatgtt gcagcagatg
      421 aattatcaag aaatacacag tcccttcaaa acagatttgc agctagaagc actttctatt
      481 tcagatagta tcattcaagc gttaaataac gatattacaa catacactat ggggttatta
      541 ggattggttg cgttgatttt tcctttattc atactgaaag aaatggagaa aaaagaaaaa
      601 atcatctatg ttggcgtagt ttctacatgg gtatgcgtaa ctaatttatt tccatggttt
      661 ttgtttcaaa aaacaccgat aagcttactc cagtttcctt ggcgtatctt aggtatccaa
      721 atcttttttt cttcaatgat cataacgctt gtttttatga aaaagactat gaataaaaaa
      781 tattcatggt taatcattgg cgctactgtg gtgtttctac tcgtcacgtc tatagcagca
      841 aaagaaaatt acgcaaaagt tatccaatca aaacacggtc atattgttat caatgaagaa
      901 acactcccac gttatacaac atcagatatg ggaggattat acgattacgc cccaacagaa
      961 gcgataaaag aaaggcctca cttagaaaaa catgaagtaa aagtaggaga atcgtgggaa
     1021 aaagggaagt ataaagctgc agataataac attacttaca cagtggcaag caacaaaaag
     1081 caaaaagtga cagtaccagt atatgcttat ttaggtacac aggtaaaagt aaacggtaaa
     1141 gtcgtaaatg actcttataa aaaacatggc ttagtgaatg tggatgtaaa tgctggagaa
     1201 aataagatag aaattacaac aaaatatact ccaactgctc tcatggcttt atggatcagt
     1261 attctgactt attgtttagt ggtgtattca agttttaaac gatttgcacc taataagttg
     1321 aaagattctc gatatgtgac taaaaaggag ataaacaaga agaatgaaaa taaataaaaa
     1381 agtagtaata ttgagtggat taattatagt tagtagtata tattctttca tctttttcaa
     1441 aaatacacag agtatttata ccactggaga tctttctttt cacttatctc gaatcaaggg
     1501 gttatcgact atttttacca gtccgattaa ttatgagaca ttcaattata ctggatatgg
     1561 tgtaaattat ttttatccct ttctgacttt ttttccagct gtaatgttat attggatgac
     1621 aaaaaatttg attgtaagct atattattta cgtttggtta ttgaacttgt gtacaacact
     1681 cgtagcttat cattatggcg agcggttttt aaaacaaaaa aaagcggcat ttttatttag
     1741 ttgtctctat attttctctg cttatcggac agtagacatc tactatcgtt ccgcaattgc
     1801 cgaagctatc gcaataacac ttgttatccc tgtattattt tatgcttatc aaatcatctc
     1861 tggaaaagaa gaaaagtatc cgtctgtaaa gctagcattg agtatgagtt tgctagtgta
     1921 cagccatgta ctatcaacat taatgagtac agcattaatt atcattttta tttttattcg
     1981 tttggtttct aaaggtttca agaatgcaga ttttattgct atatttaaaa aattatttag
     2041 tgcggcagga atgacattgg ttctgacgag tgctttttgg tatccgatgt ttgaacaaat
     2101 gctctaccaa aaaatcaaca aacctagcgt gacaaattta tatgctcatg catctaatgt
     2161 gttcgatagt ctgactgaag ctatgaacaa cgacttaact acttactcaa tgggactagt
     2221 tggtctgtta tctttatgta ttcccctgat tttatttaaa aaattaacaa acatagaaaa
     2281 aaagatttat tatggtacgt gtttgacttg gctagctaca actagtttgg tgccatggta
     2341 tcttctgcaa aatacgccag ccaaattgtt acaatttcct tggcggatct taagcttgca
     2401 aataatattc tcttctctga tattaacgat gatatttttc aaaaatagaa gatacaataa
     2461 gacaagagaa ctattttatt tatttatgac tgttttgtta ttaatcactt taactgtttc
     2521 tagtaaaaaa aatttcaatc aaaaaataat ttctcagccg ggacatattg ttgtaaacaa
     2581 agaaacagta gaggcgtata ccactaaagg aatggattat tttttatatg attattctcc
     2641 tatcgcttca ttggagaata aggagcatat aataaaacac gaagcaaaga ttggcagtca
     2701 atgggtaaac cttccttata aaacaactgg tagttctatc atatatagag taactaatga
     2761 gaagagccaa gtaatcacac ttcctgtata tgcttattta ggaacgaatg tagaaataaa
     2821 tcatcaacct gtaaaggagc aggtgaatgc taatggattg gtacaaataa gcggagtcaa
     2881 aggagttgct gatattaaag taagcacaag ttatacagta acgactatta t
//
LOCUS       DAZUKZ010000188         2899 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000188 DAZUKZ010000000
VERSION     DAZUKZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.194"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            573..>2899
                     /gene="esp"
                     /locus_tag="ACG8EF_002806"
     CDS             573..>2899
                     /gene="esp"
                     /locus_tag="ACG8EF_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFS4948046.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1054 a          441 c          567 g          837 t
ORIGIN      
        1 ttataaagaa aaagaatcaa taatttaatg aggctgtgac aaatgtcatt ttttgtttcg
       61 ctaaaaacga gtgaaaaaat gttgataact ccaatcattc gtttaagatt ctaggaatca
      121 ttcagaaatg ctgtgaaacc gatactttat aattataaag aaggtgattt caaattccga
      181 agaaggaatt tgaagtcacc tttttactta tgtcacagtc tcattcagta atgcgggata
      241 aggctcctta ttccgttatt tgaatactct gaagtttaat ctaagaaagg gtaatatttt
      301 gaaaacatgc ttcatcgcat agaaatgaac ttaatttcag aaagaataca tacatatctt
      361 tttatatgtt ttcagctgct gaatggttaa aaagcacaaa tatccgaaaa aaacatgaag
      421 aaatgtactt tttttgttgt aatgttcatg aaattctcta agtaaattag ttaacataag
      481 gttagtaaga ttcctattct gtgaataata cgtatcagat ttatctttta ttcttactag
      541 gaaagtaatt attaagggag ttgatttgaa taatggttag caagaataat aagagagtat
      601 ttctagaaaa aacaaagaaa cgagtattga agtacagtat caaaaaatta agtgttgggg
      661 ttgcatctgt tttgattggt gttggtcttg tcttgggaac aactgaacta gttaaagcac
      721 aagatgaaat ttctccaagc agcccacttg aaacagcgat aagctccgta caagtaggtg
      781 acaaagtagc ttcggggaat acttttcagg agaatcctgg ttatacgaaa aattataact
      841 tttcggattt acaatttagt cctcaagaac taactggaga cacactaaaa ggcaatacaa
      901 ttggatttga agtttacgga aaacataata ttgcagcatc aactaaaaac tgggaaatcc
      961 gtcttcaatt agatgaacga ttagctaaat atgtagaaaa aattcaggtc gatccaaaga
     1021 aaggaattgg atcttctaga cggacctttg taagaattaa tgattcgctg ggtagaccta
     1081 caaatatttg gaaggtcaat tatattcgtg caagtgatgg attatttgct ggggcagaaa
     1141 caaccgatac gcaaaccgct cctaatgggg taattacgtt tgaaaagagc ctagatgaaa
     1201 tttttaaaga gataggcata gacaatctca agaccgatcg tttaatgtat cgaatctact
     1261 tggtaagtca tcaagacgat gataaaattg taccgggtat tgatagtacg ggctactttt
     1321 taacagattc agatgatttc tataatagct tagatgtatc tgaaaataat cctgaccagt
     1381 ttaaacatgg atcagtaagt gcaaaatatg agaaacctaa tactcaaaca aaagatggtt
     1441 caggatccac aggtgctaat ggagctataa tcttagatca taagttaacg aaaaattata
     1501 atttctcata ttcatcttcc gcaaagggca cgccttggta tgctaattac aaaattgatg
     1561 aacgattagt tccatatgta gcgggtatac agatgcacat ggttcaggct gacaaagtaa
     1621 catatgatgt ttcgtttgaa tccggaacaa aagtagctga tttagcaatc gagcgacggg
     1681 aaggtcatga aaattatggc ataggttcaa taactaataa tgatttaact aaacttatcg
     1741 attttgctaa tgcaagtcca cgtccagtcg ttattcgata tgttttacaa ttgactaagc
     1801 cattagatga aatcttagaa gatatgaaag caacagcaca agttgaagaa aataaaccat
     1861 ttggtgaaga tttcatcttt gattcttggt tgtcggatac gaataaaaaa ttaatccaga
     1921 acacttatgg aacaggttat tattatttgc aagatattga tggtgatgga aaccctgacg
     1981 ataaagaaga gagcggagac acgaatccat atatcgggaa acctgaatta gaagaagtat
     2041 atgatgttga cacaacagtt aaggggaaag tattcatcca cgagttagcg ggaacaggtc
     2101 acaaagccca acttgttgat aaagaaggta ctgtattagc agaaaaaact atcgctccaa
     2161 atgaaaaaga tggggctcca atttcagata ctgtagaatt tgaatttacg ggtgtagatt
     2221 caagtaaatt aatcgcgaaa gatgaattaa aaatccaaat cgtttctcca ggttttgata
     2281 aaccagaaga aggttcaacc gttattaagg aatcaccaaa agcggttgat aaacaaaccg
     2341 tggtagttgg atttaaacca gatgctaaag aatcaattcg gaataataaa aacttacctg
     2401 aagatgcaga gtattcatgg aaaacagagc ctgatacttc taacgttact gatagtacga
     2461 aaggtattgt aactgttaag atcggaaatc gaactttcga cgtggatgta gagtttgctg
     2521 taaaagcttc tcaagctatg gaaaatgatg caacatacgt acctataaca acaactccag
     2581 aaacgacagt tcaaagtgga aaacctacat ttgataaacc agatgttcct ttagctaaag
     2641 atgccttttc aatttcagat gtctataata agtacttcgg taatgcaagt gttgatgcaa
     2701 acactggtat tgttacattc actccagcta aaggtgtagg agaatcggag ccgattactg
     2761 gaacaattcc tattaaaatt gtttaccaag atggttctgt aggcacgacc gatttagcag
     2821 taactgtaag taaagatatt tatgaaaatc caggagaaaa cattcctgca ggctaccaca
     2881 aagtaacctt caccgcagg
//
LOCUS       DAZUKZ010000189         2886 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000189 DAZUKZ010000000
VERSION     DAZUKZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.195"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            798..1052
                     /locus_tag="ACG8EF_002807"
                     /pseudo
     CDS             798..1052
                     /locus_tag="ACG8EF_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1122..1634
                     /locus_tag="ACG8EF_002808"
     CDS             1122..1634
                     /locus_tag="ACG8EF_002808"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4948047.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="ACG8EF_002809"
     CDS             1631..2668
                     /locus_tag="ACG8EF_002809"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4948048.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1041 a          513 c          574 g          758 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacaccgac catcgtccag taaagagacg caataaattc tatcgaagtt tacgcactgc
     2881 ctctac
//
LOCUS       DAZUKZ010000190         2868 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000190 DAZUKZ010000000
VERSION     DAZUKZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.196"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..1371)
                     /locus_tag="ACG8EF_002810"
     CDS             complement(61..1371)
                     /locus_tag="ACG8EF_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFS4948049.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="ACG8EF_002811"
     CDS             complement(1383..2801)
                     /locus_tag="ACG8EF_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4948050.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          897 a          599 c          447 g          925 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttata
//
LOCUS       DAZUKZ010000191         2816 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000191 DAZUKZ010000000
VERSION     DAZUKZ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.197"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(261..545)
                     /locus_tag="ACG8EF_002812"
     CDS             complement(261..545)
                     /locus_tag="ACG8EF_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcQ family protein"
                     /protein_id="HFS4948051.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
     gene            complement(566..1969)
                     /locus_tag="ACG8EF_002813"
     CDS             complement(566..1969)
                     /locus_tag="ACG8EF_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HFS4948052.1"
                     /translation="MTASDLIRNFIMMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDF
                     FRYYLAAITGEYSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPE
                     GKYSEVLSDFQSMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNG
                     DDTSRVSKAFPSYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNL
                     LNANAYAMRLARWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYT
                     GLVNTIGNLTLVTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISAR
                     SEALINELITMYPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYS
                     KIKDIASDSLDETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDY
                     WKYDNGISINDSLRNKK"
     gene            2155..>2816
                     /locus_tag="ACG8EF_002814"
     CDS             2155..>2816
                     /locus_tag="ACG8EF_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFS4948053.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSQLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKG
                     IT"
BASE COUNT          876 a          573 c          427 g          940 t
ORIGIN      
        1 tatgtgaaaa tcgaatcttg agatagctat tccccttgag atattcactg atacttctac
       61 tcaaatcatt taaattttta taattatttg tacggaacgt atatatatca acatctattt
      121 agacgttgtc ggtgatacaa tgtcattttt cccgtgtact atgtcattta tctctatact
      181 ttgtcatttt tctttttggg caatcaaaaa aggcaacaaa ccaattatga tttgctacct
      241 tctccgttta aaaacttatc ttagccccaa aatggaatcc aatttttaac ttcttttgcc
      301 aatgcatacg ccttagcaag acctgagttt tcaactaaat atttttcccc ttccagagaa
      361 acactccccc agatatgaac agtgttatcc gcccaaacca tggattctag taagccattg
      421 cgcgaaagaa atttagtttg ttctaaaata tcttcttctg gaacttgccc tttaagctca
      481 ataaaaggat tctttttttc atcacgcaat ttaagaattg catatcttag tttgttctta
      541 tccaacctaa caccacctct atgctctatt tcttatttct tagcgaatca ttaatactta
      601 ttccgttatc atatttccag tagtcccaac cattccggct accccctaat agcagagctg
      661 ctgcattcga aacagatgag gcagtgtaat cttgaacaaa ttgtagtccc ccaattgttt
      721 cttcaatgat tccattatcc aacagctctt ggcgagtttc atctagcgaa tcagatgcaa
      781 tatctttaat cttagaataa atttctgaac ctgaatgtat aatcacggtc tcgtcttcgt
      841 tcaaataccc agttgctttt attccttgcg cctctaagaa aatttcccta ttcccatcgt
      901 gttcatagtc gcccgacgat ctcaaatagg gatacatagt aatcagttca ttgattagag
      961 cttcacttct ggcagaaata tccgatgatg tccactcagt caattcgtac aaatctttat
     1021 tcatacgaat atgaagggta tcttcaaaga ttttcttttt ggtctcaaag tctttatttc
     1081 ccgcggccga attatcagga tttgtaacga gtgttaaatt accaatggta tttactaatc
     1141 ctgtgtattc ttcccccgat gtaccagcct tctcttccca atatgaagta ctagtctgtg
     1201 gcatgatgtg ttcaatggac aagttactca tatcaagtgt tgctgaattt tctcgattct
     1261 ctattttttc aagtaaccaa cgggccaatc tcatcgcata agcattggca ttcaatagat
     1321 tagatttcaa tgccttgtca gatggaagtg ccatattatt tgattgattt ctagtaacaa
     1381 gaacataaat tacaatatca acgatatttt catatccatt agctttagcg tacttcctaa
     1441 cacttttcaa atatgaagga aatgcttttg acactctgct tgtatcatcc ccattaaaat
     1501 atctcctaag ctgataatca ttcactacct taatcgcatc aatatattgg tcttcagtga
     1561 taaattggtc atgatagtac cattcagata atcccaaaac aaaaggtgca ggcatcatac
     1621 tttccatact ttggaaatcg ctcaatactt ctgagtattt tccttcaggt ttgttatagt
     1681 aaagtctcgc aaaataacca gcatatcgca ctaatttttg gagcaattcg gcatcacttg
     1741 atacatcgcg ctctttatgc caatagtctt tgaatgcttg ataaagaata tgctttgctg
     1801 aatactcccc agttattgca gcaaggtagt atctgaaaaa gtcttccatt cctttagaat
     1861 tatcaaagac ttcttccaac ctacgccaat atttagtata aagcgtagtc tgctcttcat
     1921 tagatttatc catcatgatg aagtttcgaa tcaaatccga ggcagtcaat ctttcaccgg
     1981 tagaattgat actttcaaaa atttgctgtg cattatcacg gtcatctaat ttaatataaa
     2041 caatacgcgt ggtgctagtt aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa
     2101 tcaacaaaaa acgcatatag tagtctcaaa actcccaaga aatgaggact acatatgcgt
     2161 tcatcaaaat atactgaaca ttatacagat acttttataa cattcaagga aattatgaga
     2221 actgtttcta acatatacca caattgtgta ccagataaga ttaaaaaccg aagaaatact
     2281 gatcaactga agcaacgcga tacagtgatt attgcttgtg ttatatgggg cataattaat
     2341 ggctatacta gccaaagagc cacgtataga gcggtttgtt ccgtcttatt tcttaatggt
     2401 gactttccta gtaggagtcg attcactcgc ttaagttcaa acttagctta cactatcaag
     2461 attattcgat actttttcat caagaagctc accaaaggtg aactagtcgg aattatagat
     2521 agttttccta gtcaattatg taaaccagtt agaaatagac aagcaaaact attgaatcaa
     2581 atagccaaag ttggctataa ctcaacaaaa aaatcttatt tttatggtct taaaattcat
     2641 atgatcgtta ctaagactgg ctttccgatt acttactcaa tcacgaatcc aggtgtccat
     2701 gacgtgaaag tgttagagac tttatcggaa gaagcgaatc ttcccaatat cctaggggac
     2761 aaaggatata ttagccataa aatccatgaa aagcttgccc ttaagggcat tactat
//
LOCUS       DAZUKZ010000192         2760 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000192 DAZUKZ010000000
VERSION     DAZUKZ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.198"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..368
                     /locus_tag="ACG8EF_002815"
     CDS             132..368
                     /locus_tag="ACG8EF_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFS4948054.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            387..602
                     /locus_tag="ACG8EF_002816"
     CDS             387..602
                     /locus_tag="ACG8EF_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFS4948055.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1305..2057)
                     /locus_tag="ACG8EF_002817"
     CDS             complement(1305..2057)
                     /locus_tag="ACG8EF_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4948056.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(2072..>2760)
                     /locus_tag="ACG8EF_002818"
     CDS             complement(2072..>2760)
                     /locus_tag="ACG8EF_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS4948057.1"
                     /translation="TIRQREQYEQQLKRKELVYLKKYTDSLEKDQEKFARFRHDYKNL
                     LLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKNEYLKSLLIAKLYQ
                     ANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDRELSLAIYRDDK
                     QIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNPNMFVNYQNDPY
                     QFTTQVILLF"
BASE COUNT          886 a          433 c          442 g          999 t
ORIGIN      
        1 cattcctgaa tcaaatttat tttggaaatt ttactattca agaataaact tgataagttt
       61 caggaagatt tttactgtta atcaaatacg tatgttacag tagcaccgta aaaaatatta
      121 tttaaagaag cgtgtttaaa catgcaaaat gtaaaggaac taagtattgt ggaaatgcac
      181 aaactgttgg tggaggcaat aatgcttggg gaaaattggg gcaagttgta ggtggactta
      241 ccacaggggc agtaggtggt gcaggtttag gaacagcaat ttgcggacca gcttgcggtg
      301 ttgttggagg gttatatgga gctgtcgctg gaggtgctgc agctggctgg gatgctcgaa
      361 aaaaataaaa aaggagaata agtagtatgc atattaaaaa cacaaaaaca acatttatat
      421 tatcttcaga agagttgaaa aatattcaag gtgggagtgc cgtgggagtt ttaggcacta
      481 ctttttctgg ggcaacagca ggagtaaaac tttgttcagc cggaggacct tatgcaatag
      541 ctgcttgtgg tgtaggtgga gctttattag gtgctggatt cagcatgtgg accggtgcgt
      601 aaacaattaa aaaatatcaa acctcaagat tggtttgtgt cgattacgaa cataggtatg
      661 ttctttatct tttcaaaagt ttggaatgga cagaactttt tactaaagct tcttgtgcta
      721 gtttttataa ttatattaaa tttttatctt ttattaaagt cagataaaaa ttctttggaa
      781 atgtaatggt agagatattt ttacaaaagg acttgcagcg ctgtagttgg tgctgcagga
      841 ggacctattt cggccgttac aggaggtatt ggtaatgtac ttgtcggatg tgcattaaag
      901 cctgttcctg caagttaaat ttataaaaac aggaaagtaa ttatctcaaa tcctatcaag
      961 tacaatcata ggattgcact tgacaggaca aattttttaa ctaaaaaatg aaaaacactt
     1021 tctaaaaatt agtagaattt gccaaatctt tcgcatcttc caccatacat ttaattttaa
     1081 ttccccttta tggtacttaa taaaaacaag aaaagaacta ttcatttggc ccttttttgg
     1141 ttccagacca tagaaatatt cctccagtaa ctaatattgc tgttatgcac gtaactaata
     1201 acagctataa aaattggtac aactttaata ggattcagaa aaatttgcac tagtgaagga
     1261 atctatttta ttagaaaaat gcaatctccc taaagaaaaa accactaaac agtgctttct
     1321 ttcaacttct ccttaatttt ttgtgctttc cctaaagtaa agtttctggc caactctgaa
     1381 tcaaaatata gtgaacgttt tttaaaatct atttctttaa cgttaaagat atttactaaa
     1441 cacgctcgac taattcttgt taacatagga tatttctttt caagctcact aattcttcca
     1501 taaaattcgt actgcccatc tttagtacac aatgataatc tatgtggtaa ttttgaagct
     1561 tctacaaaat aaatgtcatt tatatcaaaa gtaaatgttt gagaacctat ggaaaaaaca
     1621 aatgcttgat tcgtaacctg ttttgttgca tctatccttt cttgcgctaa taacaataac
     1681 tcaacaatct cgtttcgata ctcatctagt gtttgatctt ttgttacaaa tcctaacgtt
     1741 tcaactcttc tttttattgt tacgggtaac atctcatcat gagtagttgt aaaaatgatt
     1801 tttgcttgaa catcatattt tctgattact tctgctaatt ctataccgtt aacttcatga
     1861 tttaaatcaa tatctaagaa atatattcct tgctttggtc ggaattgttt gaggtatttt
     1921 ttaacttcta acggactttg tgttttcaat actatcttaa atactttatc gtgaaaaaga
     1981 atgaaattat caataattcg ttctaactga tttaattgta taatttgatc ttcacataat
     2041 attattggat aactcataaa tcctccttcc ctcaaaacaa caaaataact tgagtagtaa
     2101 attgatacgg atcgttttga tagttcacaa acatattggg attcttttta ttgatttcct
     2161 ctatactaga taaacctaac ccttgatgtc ccttttttgt tgttattcct tttttcatta
     2221 gagtatctat agagagtagt atctctctac aactattcga tatcgaaatt tctatctgtt
     2281 tatcatctcg gtaaatagct agagacagtt ctctatcttg actctctata gatgcttcaa
     2341 ttgcattatc gagaataatt ccaagcaccc taatacagtc aaatattgga acaggaactt
     2401 caataatagt acttggacat tcgaatttac aacaaatctt attgttattt gcttggtata
     2461 gcttcgctat caacaaactt ttcaaatatt cattttttat attttttaaa taacgatatt
     2521 caaacttatt ggttagataa gaattagaat agatctctaa ttcttttatt tctttttcca
     2581 atatcgaatc atggttttga ttagcgattt cttttaaact cagcaataaa tttttatagt
     2641 catgtctaaa tctggcaaat ttttcttgat ctttttccag tgaatcagta tattttttta
     2701 gataaaccaa ttcttttctt ttcaattgct gttcatattg ttctcgttgc ctaatagtta
//
LOCUS       DAZUKZ010000193         2690 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000193 DAZUKZ010000000
VERSION     DAZUKZ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.199"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            10..2199
                     /locus_tag="ACG8EF_002819"
     CDS             10..2199
                     /locus_tag="ACG8EF_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4948058.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2196..>2690
                     /locus_tag="ACG8EF_002820"
     CDS             2196..>2690
                     /locus_tag="ACG8EF_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS4948059.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSE"
BASE COUNT         1014 a          364 c          447 g          865 t
ORIGIN      
        1 gatctgctta tgtttatatc tactcttcaa attcgaaatt ttagaaatta tgaatccgaa
       61 atctttcgat tcgataatga gacaaatact attataggtg agaatgattc agggaaaacc
      121 aatgctctta ctgcattgag aatcttatta gatgatagtt attactatag tagtaaaaca
      181 ctaaaagaat cagatttttt tcatggaatt caaaatgggt ggcaagggca ttggattatt
      241 atctctgcga catttgaagg aatcagcgaa gaagaatttg acaatgagat ttgtgcttcc
      301 atttctttaa attctgaaag tcaaacaatc ttagaagaat tgatttcaaa tgcagataaa
      361 ggtgtaggta gtatttctct atttattaga cctaacaaag ctattagaaa gcaattattt
      421 gatatttcca ctgaattaga ctctcatcaa tttaacgaat ttagaaattc tattcgtcta
      481 tccgactatg aattttacta cacatctaaa tcaaatctaa atttctgtat caatgaaaat
      541 tatgataaat tggtaggaag actcaatgac aatatagcta gtaatcctga agaagacgat
      601 gaagctctgt tgggctcaag aattgacatg tcagatattt tcaagcatat ttcggttgtt
      661 tatgttgacg ctcttcgaga tgttcttaga gaaatgaaaa acaatcgaaa cccagtcaaa
      721 agaatcatgg aaacaattga atcaaaaata tcatcagata atgttgagtc attaaaaaca
      781 attatacagc aattaaatga gactattacc agtgttcctg aaattaggaa aattggcgaa
      841 aatattaatc ggcaactaaa tagcataatt ggttcagtgt attctcctaa tcttcttctt
      901 tcctcaacta tgtcagatga tatgggttct ttagcaaaat ttctttcaat gaagccagaa
      961 caaaatatcg acttagactt actagggtta ggacatctaa atatgattta tttagctttg
     1021 aaaatagtcg aatttgaagc gtgtcgttca agagaactac ttaatattat gttgattgaa
     1081 gaaccagaag ctcatattca tcatcatatt caaaaaactc tgttcgaagg attaaatctt
     1141 caaaaaaact atactcagat tctcatgacc acacattctg tacatttagc tgaagtgtct
     1201 gaaatatctc ggatgaatgt attaaaatcg tttaatggca aatctatcgt tatgaatcca
     1261 gcaaataatc tgaactcatt tgctaaagaa aagttaaaaa agaacaatct aaatctaata
     1321 aacgcagtag agagatattt agatgttaaa agaaatggat tgcttttttc taaaggtgtt
     1381 atcttagttg aaggagatgc tgaagaaata cttattcctc aaatagtaaa aaaattattt
     1441 ggtataaact tagacgaact tggaataggt ttgattaatg ttggtagtac ttcgtttgaa
     1501 tatatcgcta gcttgtttga cgatgataga atacagagaa agtgtgctat tgtaactgat
     1561 ttagatgaac aggcaatccc atcagaacat cgattgtata aaagcactgc acaagagaaa
     1621 ggtcaagcaa gaaaagaaaa attatcctcc ctttttaatg aaaacaattg ggttaattgt
     1681 ttttatgcta atacaacatt tgaaattgag tttttagatg tgaataaaga aaatattcta
     1741 acttatgtca gtccaattat agatgacgca ttcaaagata aaaaaacaat aactgcttat
     1801 aaagaagaaa tacaaagtga acaatattta gaaagaaatt tagcgatgtt aaaactagcc
     1861 aatcatgttg gtaaggggtg gtttgcaatt gagttaggtg aaaaaattga ctttttaatc
     1921 aaaattccta agtatattct taatgctatt gcttttgcat gtcaggaaat agtagatctc
     1981 tcaatttata aaaaaattct actccataca atcgacttgt acgaggaaac tacagaattg
     2041 tcagctttat cagctagttt aaaaacaagt gatacagctg atttgaatat agcaataatt
     2101 tctttattag agtactttgg taaagatgag gatgttgtgt atctaatcaa tcagatagaa
     2161 aattacacaa aaatacttta tgctgaggtt tcaaaatgac aaatatcttc gaaaaaatcg
     2221 taaatgattt aaatttagaa caaaaagatg ttgtggaaag tgaagataac ctttatgtaa
     2281 atgcatgtcc tgggagtggg aaaacaaggg tacttacaag gaaaattgct taccaagcaa
     2341 ttaaacacga tggatcacta aaaagaataa ttgctatcac atacactaat agagctgctg
     2401 aagaaattaa ggaacgccta agtttactta gtatagatga tgatattaat atttgggtgg
     2461 ggacaataca tcagttttgt cttgaattta ttatttatcc ttttaaaatg aatctctcaa
     2521 gagtatcaaa aggatttaca attattgatg attatactca aagagcttac gtcagccaaa
     2581 ttaataatat gctgaattta aatctcaaaa cgtatgaact gaacaaaatt gatttgtcac
     2641 tttctcatga ttttaaaatt aatgaagttc agtatactca agtttctgaa
//
LOCUS       DAZUKZ010000194         2552 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000194 DAZUKZ010000000
VERSION     DAZUKZ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.200"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..588)
                     /locus_tag="ACG8EF_002821"
     CDS             complement(<1..588)
                     /locus_tag="ACG8EF_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFS4948060.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYL"
     gene            681..1030
                     /locus_tag="ACG8EF_002822"
                     /pseudo
     CDS             681..1030
                     /locus_tag="ACG8EF_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1302..1592)
                     /locus_tag="ACG8EF_002823"
     CDS             complement(1302..1592)
                     /locus_tag="ACG8EF_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948061.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1800..2492)
                     /locus_tag="ACG8EF_002824"
     CDS             complement(1800..2492)
                     /locus_tag="ACG8EF_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4948062.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          754 a          479 c          368 g          951 t
ORIGIN      
        1 caaatataaa ctaactatac ttgttagaac aaaaaaacag gcgttgactt gttcttgatt
       61 agttaaaaat atcaataaag gaatacttac ccaatatatt tttctattta tttcttctcc
      121 tcttaagagt tgaaaaaacg gataaaaagt tagtaaacta aatgctactg gccactgata
      181 gttcaaggtg gttgctctcc aaccggtttc ccccattact gtcaatggta ttaaacagta
      241 tatagagcaa accaataata aatttttaac atttattttc tcaaaaacat acttgcaaag
      301 taatactaca attcctaaca taatcgtaga atttaataat ttccatagca taaagtgttt
      361 agaaaagaac attaagaata gttctataat aattcttgaa ctccatgtct tataatgttc
      421 tatcgaagca cccaaatagc caagttcatc taatctactt gcataaacta cgtcatccgc
      481 tctcatagtc ggcatataca tattaaagag taaaaagata ccccaaatcc ctattagtaa
      541 caagatcagt tctttgttat tcgttatttt tctattatgt ttgttcattt ttatactcct
      601 tcttatattt cctaatagag tataacatag ctttaagcat tacttaaaga taacttaaaa
      661 gttatctttt aaatacttaa ttgttaaata ccttatttga tgcaaaagaa atctacgata
      721 gtcaacaaaa aaattttcat tatatccaga aaaatttttc ttagttaagg acctatggat
      781 tgcagtaatg cagaattcgt cgaataaact gccaaagaca tacaatcata ttgcttttaa
      841 aattgacgaa caagatatag attcgtttgt ctcaaaaatc caaatgttag gtttaactgt
      901 cgaaccagga cgttctcgtg tgaaaggcga ggcttcttca atttactttt acgactatga
      961 taatcacctc ttcgagttac ataccggtac tctccaggaa cgtttaaaat cctacaattc
     1021 cactacctga gactagtaat gactagtctc tttttatggc ctctgatccc cccattgagc
     1081 gaatactaat agggcttcga attgatctgt tcaatcttta agcaatatcc ctcaaatgag
     1141 tttggaaagt ttagtgattc atcacaaata cttaaataca tgtagttata tatagattga
     1201 ggttcttgtg agcgtatgct cgtgtgggtt caaatcccat cgtctgcatc catctttcca
     1261 aaacaagcgg ctaggacatt tttgtctcag ccgcttgttt tctagtttat tctttcatct
     1321 tgtcggacct tttttcttgg gtatagactt ttctcatatt ctaccacaat tttgggtttc
     1381 cctaacacat gataggatct cacaacaaat tgctttctat tattataata acctgtaacg
     1441 actaacttca tcccttcgtc tacttcagct aggaagttca aactatggga agcaatcaaa
     1501 caattgaccc catttaaact gaaacggatc aacggtctgg cactaacttg caaagtacgg
     1561 atcttatcta ctgttccata ttttttctcc ataaccttca cctcaatacc attatacgaa
     1621 catacattcc ctttgtaaag aagaaactct gttttatctt tttgactttt tgtctattac
     1681 ctttcttaaa atcatattgg cacttcctgc gaagatgaag gacatgtact aaaaaaaaga
     1741 ggcagcagta tagattactg ttgcttcttt actaatttag gtttacttgt taagaaagat
     1801 tatttacgca aatgttcttc aacaatttgc tgagatttat cagctgtatc ttccacttca
     1861 tcttttgctt tttctaactt ttgaatatat tgtttcaagc ttgagttttc agcagttgct
     1921 tcgtctaatt tcttttgtgt atctgttaac tgagtttcta catttgtttt ttcttgtgtc
     1981 agtgttgcga tttgttgatt caaaccattt acagtatttt gtaattctga aagttttgct
     2041 tgtgcttctt gtagtcttgc tgtccaatct gcattcagat gattgatttc atcatttttt
     2101 tggttgatct catctagttt gacattgatt tcattttgtt tgctttgtag ttgtgcatcc
     2161 ttgctgacta attgactttc taagttttgt ttttctgctg ccgattgatt tttttggttt
     2221 tctaattcat ttacttttgt ttggtattct tggacatctg cctttgaatc ttgcaatgtc
     2281 tgttcttttt gtgctagtcg agcggtaaga tctgtaagcg cttgctcttt atctgtcaat
     2341 gtatgatcaa gctgtgtcag attctcattg attgtatcaa tatctgtctc tccaccccac
     2401 aaaataattt catttgcgta ccgcgtgcct gtgtagccaa ttagacatag aaatgcaacg
     2461 acaatcccac cagtaataaa tttctttttc atttgcatag ctccttaatc caatactttt
     2521 acatgctaaa agtaagtgat ctcaaacaca at
//
LOCUS       DAZUKZ010000195         2544 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000195 DAZUKZ010000000
VERSION     DAZUKZ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.201"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            257..532
                     /locus_tag="ACG8EF_002825"
     CDS             257..532
                     /locus_tag="ACG8EF_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948063.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..>2544
                     /locus_tag="ACG8EF_002826"
     CDS             545..>2544
                     /locus_tag="ACG8EF_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS4948064.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYT"
BASE COUNT          766 a          397 c          555 g          826 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atac
//
LOCUS       DAZUKZ010000196         2511 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000196 DAZUKZ010000000
VERSION     DAZUKZ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.202"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            36..1006
                     /locus_tag="ACG8EF_002827"
                     /pseudo
     CDS             36..1006
                     /locus_tag="ACG8EF_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          919 a          342 c          496 g          754 t
ORIGIN      
        1 agttccgacc aaagaattat aaggagtgag tacaaatgac ctatacccat cttacaacgg
       61 atgaacttgt aataatagag tcttatttca aaatgaatca atctgttgct aaaactgccc
      121 attgcttgaa tcgttcaaga caaacgatcc ataaagtata tctgttcttc aagcaaggaa
      181 aatcagcctt agaatattat caacagtata agaaaaacaa atcaaactgt ggtagacgtc
      241 cgcttgtttt acccgaggaa caatcagaat atattcaaag aaaggttgtt caaggatgga
      301 cacccgatgt gattgttggt cgtgcagcgt ttcctattag ttgttctgct cgtaccattt
      361 atcgtatgtt caaaaagggg ctatttgatt cttctgactt accgatgaaa ggcaagcgta
      421 aaccgaatgg acatcaagaa agacgtggaa aacaaacttt ccgccgctct attcatgaac
      481 gtgaaaagga ttatagccaa ttctcaaatg agtttggtca ccttgaaggt gacactatcg
      541 taggtctgaa acataaaagt gctgtaatta ccttagttga acgattatca aaagttatca
      601 tcacattgaa accgtgtggt agacaagcga ttgatattga aaaaaaatta aatcaatggt
      661 ttgaatctgt accgaaaaac ctattcaaat ccatcacttt tgattgtgga aaggaatttt
      721 caaattggaa acagatcagt aatgtcaatg atattgccat ttatttcgct gatccaggaa
      781 cgccgtctca aagaggccta aacgagaatt ctaacggatt gttacgtaga gatggtttat
      841 tgaaatctat ggatttcaat tcagtagatg aattttttat tcaatctgtc gcatctaaac
      901 gaaataatat tcctagaaaa tcactggatt atcgaacgat tatcgaacac ctttggaagt
      961 atttttgagt tacgtaagta ttgatgatct gtctaattta atttgacaat taagaaagta
     1021 ttaacttgtt ttgtgaaaaa gagaatttga aaaacgattg gcgaacaaat gctcggtagg
     1081 tatggtataa atacagagct aattggtgtg atgaaatcag atattcgcgt gagattcagt
     1141 aaagtaggag ttcgaaaaca gctaggtgac aaacagtatt gctttggtac ttcggaatgg
     1201 tttatcaaat tcttatcaac agttcacttc tctttatgtt tgagtgatga ttcgttaggt
     1261 tattgcttgg aaagaccgga tgagagtaga agagtttaat tttataaagt gaaaagaggt
     1321 aatgtacgat atacgtaggg ctgaaccaat agaggataag caaacgatgt tacaggcgca
     1381 aaattcatag cttactgttt tggagataac agaagaggaa ataatgcaga agtaaggcga
     1441 aaaaaggacg gaagcctaac agcatatgaa ataaagaaaa atattataac tactgataaa
     1501 tcggtattga gcaaagtact aataggagtc aaaaacattc ataagctatg agtgtttctt
     1561 ggctactttt ttatttttat gtatctacaa aagtttccag gggcagaacc tgtgattatt
     1621 aatggaagaa cctcaagtat tgtcttaaat cattgttatg aaaatggatt aaaagagttc
     1681 atcaaggtgt gattaacaag ttacttttca ttacatcgaa tttttcagaa gttgcttata
     1741 ttggtgatga tattaatgat ttatcatgta tgacatctgt gaagaagcgg gaggaatagt
     1801 agaatgtcca aaaaatgcca caagaaatga gattgaatta tctgatttta ttgtaactca
     1861 cagtggtggt gacgaatctg tgatagagtt tattgagtgg atattggaat cgatatagtg
     1921 tagtagtaca gaaaaattat catacaatta atactaggca taagaatatt ggcatattct
     1981 agagttaaaa gtaactattc gaaaaggata aggttagaat tttttgtttt gccagtggaa
     2041 gaatataaaa agggcattgg aaattaatat attagaatgc taagcggaga aaaaaatata
     2101 tgaaaagtac gtccttgtga tgggatggaa aaatacactt atttcaaaca acgataatat
     2161 aattatgtga accgaagtat actcgcttct aattcaatcc tcaaatcccg acagtcgcga
     2221 cttcaaaaaa gcgtatagtt ttaatgtatc cccatatgat ccgaaaaatg gtaatttaca
     2281 attaacagtt tgatgatgtt gaagatgtgg aaatgctgaa attaccatga tgagaaacac
     2341 attaattaaa gggaattaat taactagaat ataatctgat tcaatttgaa ttaagcaaaa
     2401 aaaggctgag ttttaaataa tcgcgaattg taaaattaag ttagaaaaat aaaaaggcat
     2461 ttatggtaca ctcaaattgt atttccgacg aaagaaaaca aaggagtgta a
//
LOCUS       DAZUKZ010000197         2486 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000197 DAZUKZ010000000
VERSION     DAZUKZ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.203"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..464)
                     /locus_tag="ACG8EF_002828"
     CDS             complement(243..464)
                     /locus_tag="ACG8EF_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948065.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="ACG8EF_002829"
     CDS             complement(562..807)
                     /locus_tag="ACG8EF_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948066.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1047..1661
                     /locus_tag="ACG8EF_002830"
                     /pseudo
     CDS             1047..1661
                     /locus_tag="ACG8EF_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2031..2273)
                     /locus_tag="ACG8EF_002831"
     CDS             complement(2031..2273)
                     /locus_tag="ACG8EF_002831"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948067.1"
                     /translation="MSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFK
                     RYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          850 a          429 c          348 g          859 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taacttttag aattcaaaaa ccgtttgtct atttagagtt taccctaaat
      961 agacaaaaat ataacattga aaaatttaga gttctaacgt atgattaggg tgtattataa
     1021 acgttataga aaagtaggga ataaatttga aaattgctta tattagagtt tcatccatcg
     1081 atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta
     1141 tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa gctttagatt
     1201 ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga cgcaattatg
     1261 acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt attatcacca
     1321 gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc atcaaagact
     1381 taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg aaacgacgcc
     1441 aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt cctaagttat
     1501 acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc gttcaggatc
     1561 ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact aggcaaacaa
     1621 tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt tgaatcacgt
     1681 aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc tagctaagtt
     1741 gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc ataaatcgtc
     1801 cttatttaaa ggcatttaac attgacaact tagtataatg tatcttatgt aaactaataa
     1861 gatttgaatt ttattttata cacactcgat aatagatcaa ttttatggtc ccccgcattt
     1921 acgattgcac aagagaaaca taaagtcgac ctattagcat ttcttttcaa ttcaaacatt
     1981 aaaaaagttt atacctctgc tactaaaaaa tgaaattatc tatttaaaat ttaaaacggt
     2041 aaatctgatt catccaaatt ggaaaatgga tcagcctgta ttggagcttt gtatttctcc
     2101 ataataacag atatcttttt ttcatttttt aaaaaatacc ttttaaattt cttatagtcc
     2161 tctgaaatca aaatatagcg tgatggatac tcatcaaaag aagaatcgta ttcttccttt
     2221 aaaattcccg atttacttaa tttttctagt acatcaaata ctctatcact caagtaactc
     2281 cttaaattgt tatcactttc ccattgaact acttttctat aagttgaatt atcagttgaa
     2341 aagatagaat tttcatccat aaacgcatat attagcagca atctttcatc attgtcaaat
     2401 tctattggat gaatcggcta cactagacta gacagaaaaa ataaggtgtg tagaataata
     2461 aaaaacacac tggaggattt ttcatg
//
LOCUS       DAZUKZ010000198         2462 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000198 DAZUKZ010000000
VERSION     DAZUKZ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.204"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACG8EF_002832"
     CDS             <1..435
                     /locus_tag="ACG8EF_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS4948068.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACG8EF_002833"
     tRNA            complement(759..842)
                     /locus_tag="ACG8EF_002833"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACG8EF_002834"
     CDS             1370..1870
                     /locus_tag="ACG8EF_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4948069.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACG8EF_002835"
     CDS             complement(2060..2404)
                     /locus_tag="ACG8EF_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFS4948070.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT          765 a          476 c          407 g          814 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 ta
//
LOCUS       DAZUKZ010000199         2457 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000199 DAZUKZ010000000
VERSION     DAZUKZ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.205"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..2306
                     /locus_tag="ACG8EF_002836"
     CDS             153..2306
                     /locus_tag="ACG8EF_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFS4948071.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          839 a          428 c          544 g          646 t
ORIGIN      
        1 gatatgtaac tttaagtgat tgctgtgaaa caaaaagttg attttatctt gaaattaggt
       61 aaagaatttc ttgctccgcc cattttttct tttttattaa gaagtttttg ggtacaatga
      121 agcatgaatg taaataggcg gaaggatagg aaatgatgaa agattttatg aaaaaaatat
      181 taaatacttc ttggttagaa aaaataagaa aaggtgcaca agaacctaag aacaaaacgt
      241 ttcgccggtc ccatgttcct tttcgcttaa attttctttt tttcattata tttacgttat
      301 ttgttgcgtt gattgctaga ttgggttatt tgcagatcgt gaacggcgaa gagatggagg
      361 cgcgagtgaa gtctacttct acgattacgg tccaagaaag tactcctcgc ggaatgatct
      421 acgactccac aggaaaagct ttagttacga ataaagcgaa tcaggccatc acatatacga
      481 gaggaacaca agttacggct aaagatttgc ttgaaatcgc aacgaagctg aactcgctga
      541 tcgatgttcc ggtagatgaa aatctgacag atagagataa aaaagatttt tggctggctg
      601 atcccaaaca tttgaaagaa gccacaaaaa gactttcggc aaaagaaaag caattaggga
      661 acagcgaaca atatagtgcg atggtcaata aagtcacagc tgatgagatt aatttaaacg
      721 aggagcaatt aaaaattgct accatcttta aacggatgaa tagtgcatat gaattaaaca
      781 ctgtctatat caaaaactct ggtgttactg atcaagagtt agctgttgtg gcagaacgcg
      841 cttctgaact tccaggagtt tcgactggca cagactggac acgagaatat aatgcagcaa
      901 gttcactgaa aagtattctt ggcagtgtga cgactgaaaa gcaaggactt ccagcagatg
      961 aagcagagaa atacctagca aaaggttatt ctcggaatga ccgtgtagga caaagttatt
     1021 tggaaaaaca atatgaagat gtcctgcaag gaaccaaaac ccaatatgaa gtttcccttg
     1081 ataatgaagg aaatgtttcg aatcaaaaag aaatcttctc tggggaaaaa ggttctaatt
     1141 taatgttatc tatgaatgca gaattccaat caaaagtaga agaaattttg aaaaggaatt
     1201 atcaaacatt gatcaacaac ggcaaagcac agtattcgcc tggtgcttat gcagtggcta
     1261 tgaatcctca gacaggagaa gtgctagcaa tgactggttt cagtcatgaa caaggttcca
     1321 aagaaatcac cgagaatgct ttaggcacga ttactagcgc ctttgatcca gggtctgtag
     1381 tcaaggctgg tactttgaca gcaggctggg caagtaacgc gatcagcgga aatcaagttt
     1441 tgattgatga gccgattcgt ctgcaaggtg cttcagaaaa aagctctgtc tttaatcgaa
     1501 gcggacaagt tgctcttgat gcagtcaaag catcggaatt gtcttctaat acgtatatga
     1561 tcaaagtggc gttaaagatg ttaggactag actatacacc tggaatggga ctacctagtt
     1621 tagatgaaga agctaaagct tatcagcaat tgagagactc tttcaaagag tttggtcttg
     1681 gtacaacgac tgggatcgat ttgccgaatg aatctcctgg gatctcacgt tctgttgatt
     1741 atatgaagaa gttcaatgcg gataatggca aagaatggta tgctccaggt aactttaccg
     1801 atttggcatt tggacagttt gacacatata caccaatcca attagctcaa tatgcttcaa
     1861 cagtcgctaa tggcgggaaa cgagtacagc cgcacctagt caaagcgatc tatggcaatg
     1921 acgaaaatgg aaacttgggt gaagtgaaga aagaaattgg cacaacagta gaaaatacgg
     1981 tcaatatttc tgcagaaaat atggcgatcc tccgtgaagg attccaccaa gttgtccatg
     2041 gtaccgatcc ttacacaaca gccaaaccat tagccagtgc gaaaatggac ctttccgcaa
     2101 aaacagggac tgcagaaacg gttgcggaag gtcatccaga tattgcgaca gtcaatagta
     2161 atatcgtggc atacggacca acagacaatc cggaaattgc catcagtgtt gtcttgccga
     2221 acttattaga cgaacaagac cacataaact tgacgattgc taaagaaata atggacgcgt
     2281 attatgatat gttcatggca aaataaaaaa gaggtcgtga aaaagccgct tagtttcgag
     2341 tatgaagaaa aattttgtga aaatagcttc tcacattttt gcttaaattt gacttcatac
     2401 cgagaaactg gcttttgaac accgtttact cggcaaaaaa ggttgtgaca aaaattg
//
LOCUS       DAZUKZ010000200         2407 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000200 DAZUKZ010000000
VERSION     DAZUKZ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.206"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1150
                     /locus_tag="ACG8EF_002837"
     CDS             5..1150
                     /locus_tag="ACG8EF_002837"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFS4948072.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="ACG8EF_002838"
     CDS             1321..1779
                     /locus_tag="ACG8EF_002838"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFS4948073.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="ACG8EF_002839"
     CDS             1938..>2407
                     /locus_tag="ACG8EF_002839"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFS4948074.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DAZUKZ010000201         2322 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000201 DAZUKZ010000000
VERSION     DAZUKZ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.207"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..1991
                     /locus_tag="ACG8EF_002840"
     CDS             129..1991
                     /locus_tag="ACG8EF_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4948075.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            1991..>2322
                     /locus_tag="ACG8EF_002841"
     CDS             1991..>2322
                     /locus_tag="ACG8EF_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4948076.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          835 a          344 c          455 g          688 t
ORIGIN      
        1 gtctttataa tagataaact ctgtaatatt agttaaaaag ctataagttg cacttagtta
       61 agtgcaaaca tttcatattg tcgttagttt agaaaacgct tatattagca atgtaaggag
      121 agatgataat gcagcagcta acaaaaagag aaaggcagct tttgatttac ttggtggaaa
      181 tgtctgaacc ggtaacaatc aaaaagttgg ctgaaaaaga aaatgttagt gttcggacga
      241 taaaatacga tctagatgat ataagagagt ggctgaatga gcggaagcaa gtcctatata
      301 gcaaacgtag tcagggaatt tggatgaata ttagtagctc tgatcgaatc aaattaaaaa
      361 gcgaattgat ggatgttaat cgttttgaaa tatttgcaga tcaaagtctc cgaatcaaac
      421 gtttactaat tatgctttta gtaacaaaag aagctattac tggaatgaaa atggccaacg
      481 cattagatgt cagcaaagat acgatcatga atgatttaga tgtgctggaa aataattttc
      541 ttaaggaagg attgaccttg aatcgtcagg cgcgaaaggg tttttggatc ataggagaag
      601 aacgtctagt tcgattaacg attgaagaga ttcttcaaaa agagttcacg gattatgaca
      661 tttataaatt aatgtcattg ttattgaatg gcggtgagac tgaatacttt gaaatgtatt
      721 ctgctactgc tacacctatc caagaggtat ttaatcaagt aatcattcga atgcgacact
      781 tactggaatt tgaaaatcta gagaaactga attacgcgga attactaaat attctgattc
      841 gagtgacaat tgctaccgtt cgcttacgca aagaggctac gattggtcgt tatcaattgg
      901 ttgctgaaca agaattagac aaaaacgatc tttcttatcg cgtcatgcag caggtctttg
      961 attattatca gttgccactg ttcgaggatg aatatcatta tatctatagt gatacattcg
     1021 acacgattca ccagcaagac gtcatgaaac tagttgagaa tgtgatttat aaagtaagta
     1081 aagcaatcaa ccattctttt tatcaagatc cacaattgtt gacgaatctg tatgctcatt
     1141 tatccatgcg attatcgaga aagaaaaaat ttgtcaatga atataatcct tttaaagaag
     1201 atatcaagca gaaatacccg gaattatttg atgcgatcaa gcaagcagtt cgacaagaaa
     1261 taagtgaaca aaatattctg atcaatgatt ctttcgtggc atatattgcc ttacactttt
     1321 tggtttccta cgaaagagaa gaagatctac gaagcgttag agccgtctac atttgttcaa
     1381 cagggttggg tgtgactagt ctgatcaagc aaaagatcaa tgaagaatta aataatatca
     1441 ctatcgtggc atttgcttca gtattgaatg cgcaagaaat gattgctacc aaagatcctg
     1501 atttggtgat tagtattttt ccaattaatg gcatcgatcg tcctttcatc aaagtccatc
     1561 cattacccac tgaacaggac ttgaaaaaaa ttcaacaaat aataaaaaaa atacttgctt
     1621 cttccaatca gcaggaaagg cataacttga atcttagaga aacgtccatc aataaagata
     1681 ctttagaaga ctttagcaag gaattgattt tgaaagctta tacgatttat gaacgtttac
     1741 ttgtcatttt ggaagcagaa ttgatccaag aatatcgaga agcttttttg cttcatgtat
     1801 tcttaatggt tcatcgtatc acatttgatc aacaatatat ggcagaaggt cgtcaagtag
     1861 accagattat tttagacaaa ggaacactgg taggaaaaat cgaagcagtt tttgcagaaa
     1921 ataatttagg aatcaatcag tcagaaatca gtgcattatt ctcatatata aaagaaggag
     1981 cgtcaagtta gtgaaaatgt cagacgaggc tttaaaaatt atcgaggaaa gtcaaaatcg
     2041 ggaaaagtta aaacaagtga ttgatctaat tgaagagcga ttagtagaac atgctattgt
     2101 accaaccgag ttgcagtgga ccattctaat taatcacttg aatgagatgc tgaaacgttc
     2161 tcaaagaaag gaaacgattc ccgtagtcga tcgggatttg ttcaaagaag tatccaaaga
     2221 agccataact atttctgatg aaatagttag aagtatcggt aatttaaccg atgatgaaat
     2281 atatgttttg tcgattcatt ttgaaacagc aaaaaataat ta
//
LOCUS       DAZUKZ010000202         2203 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000202 DAZUKZ010000000
VERSION     DAZUKZ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.208"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(147..617)
                     /locus_tag="ACG8EF_002842"
     CDS             complement(147..617)
                     /locus_tag="ACG8EF_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948077.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            704..1402
                     /locus_tag="ACG8EF_002843"
     CDS             704..1402
                     /locus_tag="ACG8EF_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFS4948078.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            1580..1921
                     /locus_tag="ACG8EF_002844"
     CDS             1580..1921
                     /locus_tag="ACG8EF_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948079.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1914..>2203
                     /locus_tag="ACG8EF_002845"
     CDS             1914..>2203
                     /locus_tag="ACG8EF_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS4948080.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT          805 a          314 c          444 g          640 t
ORIGIN      
        1 aagcgttgat aggaacgatt gtttacatgg tttggatttt tatggggctt gatgaagctg
       61 aagctgagta cagagcgaaa aagccaaccg agggggctga ctaaaaatga atagaaaaga
      121 gaaactagaa tgtatattac tattactcag tttaattctt tcactaattt ctctattggg
      181 aagtttgtat ttttgatatc aacaattttg tggatttggg ttctatttag agtaaatatc
      241 atttttgttt ggtgattaag gagtttatta aaattaattt tccctacttc aaatgcaagg
      301 taacaagata tagcactttt aggaggaata tttagcggta cagcttcaga gtatattcta
      361 ttatcatcga ctgatcctcc tttagcaatt aatttcttaa atggagtagc tgaccatctg
      421 cggtttaagt tagcggatga aagttctaaa ttaactaaca tagctggttc tgttgaatag
      481 ttagaaatga ttacttttgt ataaatcata tctgcagcga agtaagaagc attaagttca
      541 acttctagtt gagggcgcat tccttttatt tttatccctg ttaaaaacgt actcaatata
      601 aaacttatta ttgacatcca ttgaaaaatt gttaagtgaa aaaaattcaa aataaacacc
      661 accagttttt aactaaatta taccaaaaag gagagaagaa ataatgcaag aattagtaat
      721 tttgaaaaat aaagaagctg tgactacgag cttacaagtg gcagaaagct tcgagaaaaa
      781 acatcaacat gttttaagag atattgatgc actaaaaaaa gatgtgtcca attttggaca
      841 gatgtttgta gaaggtaatg aaccagattc atatggcaga aatcgacgag ttttcttcat
      901 tagtagagat ggatttttct tgctggctat ggggtttaca ggaaagaaag ctatctactt
      961 caaacaaaaa tacattgaag cattcaacga aatggaagat gttattcgca agaatactgt
     1021 tcctcaaaca attgaagata tgatgatcta tcaattagaa gaaatgaaag atgttaaaaa
     1081 agatgtttcc atgcttaaag atactatgcg aattagcgga caacaagagt ttgaaattaa
     1141 gcaaaaagga aatatgaaag ttatgggagt tctaggggga aaagaaagcc gagcttatga
     1201 agaaatcagc aaaaaagtat tctcaaaatt ttggtctgaa tttaaacgta ccttttcaat
     1261 cccaagatat ggcgagttac ctcgtaagag attcgatgat gctgtttcat ttattgaaat
     1321 gtggttgcca gaaactgcga tccgtatgga aattgatcaa ctgaacagac aacagagact
     1381 ttttggtgat gacaatgaat agagctgaag cgctaagaat agggacggca attgctaatt
     1441 gctggtggaa atactataaa ccaatcatcc taagccaaca acatattgac aagcaaaaag
     1501 catggcaaca aataaaaaag tgactccgcc ggcaagcaaa gagtcacaaa gaaaacacat
     1561 cataaggaga ttttagcata tggaaaaaga actttccact ctagatcaat atttgattga
     1621 tcctgattgg ggcaagccga aaattgagga aacaagtggt cgaaaaatca gacgaaatct
     1681 tttgacgaat gaagaactag cttgggatca agatgattta ggcaaccatg taactatttg
     1741 ggatcatgtt tatcttatcc atctatcgaa gcattcgaat aaacctgaat atatttacgt
     1801 catcgaagat ggcttgattg atgcgctaga agagtacgac agagataact tgattgatat
     1861 ctcttattac ggaccaggta agaaatacat tgctgaaatg gaggcagaat ttgatgagtg
     1921 aaatcaaagg gacaacgaac tttgaaaaac tttttagtcg taagttaaat aaaattctca
     1981 agaaaaaagg aaattttgat tatttatctt gggctcacgc gtgggagatt atgaaaaaga
     2041 atgatccaca ggcaacggta actattaatg agtacaaaca ctacagggtt gtttctggaa
     2101 ctcatcaaga ctttcttgtt gaggaatata aaccttttct tatggatgaa actgggactt
     2161 atgtatctgt ctcagtaacg gttaaaggac acacggaaac cga
//
LOCUS       DAZUKZ010000203         2174 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000203 DAZUKZ010000000
VERSION     DAZUKZ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.209"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            371..897
                     /locus_tag="ACG8EF_002846"
                     /pseudo
     CDS             371..897
                     /locus_tag="ACG8EF_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1052..1627
                     /locus_tag="ACG8EF_002847"
     CDS             1052..1627
                     /locus_tag="ACG8EF_002847"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS4948081.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1670..1867)
                     /locus_tag="ACG8EF_002848"
     CDS             complement(1670..1867)
                     /locus_tag="ACG8EF_002848"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948082.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            1833..2036
                     /locus_tag="ACG8EF_002849"
                     /pseudo
     CDS             1833..2036
                     /locus_tag="ACG8EF_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          768 a          344 c          405 g          657 t
ORIGIN      
        1 ctcttttcta caagacaaag tatagcaagc aaaattcttc tccacttcca gaatttttta
       61 ttttcatttt tagaaaagtg aaaaaactga caacaaaagc ttgtttcttt cactctatta
      121 atatctttag tgaaaaagct cgttttatat cgtattcttc cagcgaatgt acttctcata
      181 agtccaaaaa aagctgcgaa aaagaaagta cctcggtacc tactttttcg tagctgcaca
      241 taacttgagc aagtattaat cgaagatact tttgttgaga gtgtaaaata ttttgtgtaa
      301 atagaaaaaa ggaagtccct tctgtagaat agagttacca caacacattc acagaaaaga
      361 ggacttccat atgaacgatt ttactacaga aatcctaaag actctagcga acaaaggcga
      421 tttgaatgga ttattccgtg tccatttgga aaaagctgtc aatacgcttc tcaaaacgga
      481 gttaacggct ttcctcgatt acgaaaagta cgatcgcatt ggttttaaca cgggtaattc
      541 tcgtaacggc tcctatgacc gtacggtcaa gaccgagtat ggggaacttc atctccagat
      601 tccgcgcgac cgcaatggcg agttcaagca acagactgtt cctgcttata gacggacgaa
      661 tgacacatta gaggagaccg tcattcacct cttccgaaaa ggtattacca tgtcggaaat
      721 cgcagacttg attgagaaaa tgtatgggca tcactacacg ccccaaacca tgtccaacat
      781 gactaaagtg ctgactgaag aagtaaatgc atttaaagtt agaactctaa atgataagta
      841 tgtcgctatt ttcatgattc cttcagtttt tcagatgata taccactcaa aaaacaatat
      901 gagagatgtt caggaatacc atctctaatg gcttataaat tggttttagg gatagtagat
      961 gttatagaca tatcaaggaa taaacgagca gctgcataaa agcagatgga attaaggaat
     1021 aattgaattg taaaagaagg gaataaattt aatggcacaa ttattttaca gatatggaac
     1081 aatgaatagt ggaaagagta ttgagctact gaaagtggct cataattatg aagaacaagg
     1141 gaagagagtt atcataatga caagcgcaat agatactcga tcaggatttg gaaaagttga
     1201 gagtcgtata ggtgtttcga aaccagctat tgcaattact tctgaaatgg atgtgtacgg
     1261 agaattagtg gagattattg atcagtacgg aaaagttgat tgtattttgg tcgatgagtc
     1321 tcaattttta agtagagaga atattattga tctatcactt atcgtaatga atgaggaagt
     1381 accagtaatt tgctatggat tgaagaatga ttttcaaaat aatctcttca agggatccga
     1441 agcattattg acatttgctg ataaaattga ggaaattaaa actatttgcc atttttgcaa
     1501 taaaaaggct gtaatgaatg ctcgtattgt tgatggtatt ccagtttatt caggtgaaca
     1561 gattgtagta ggaggtaatg aagattacct ttcggtatgt cgcaaccact actacaacga
     1621 agattgaaat aaaaaacgtg taatcaaata attgattaca cgttttttat tattctgtta
     1681 aaggttgagg atcagaaatt tttaggtatc tataatcctc acctaaaaat ccgttaaaac
     1741 gaagtaaaac atcatgacca tttaaatcag ttggaactaa aattggaatt aatttatcaa
     1801 cagtagtttt gttgtaaagt tcaaaagtgt tctccattcc ttctggttct acaatcgaat
     1861 ctttcaataa acaaatcaag aaatacagcc gcagaaaaga gcagtttcaa aatgaagaat
     1921 cactagagcg tttcctagta tccatctttg atacatacaa tcaaaaattc ttaaatagaa
     1981 gccataaagg cttccaacaa gtgacggata cattagcttc aatgtttact gagtaaccca
     2041 ttattttgca gaaggacgag ttatttacac aaaattattg acgctccctg tttggagtgg
     2101 taaaaagatt gagaaattgt ttcattagct attacgatta ttattgtaat atatttttat
     2161 tgaaaatcaa aatt
//
LOCUS       DAZUKZ010000204         2171 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000204 DAZUKZ010000000
VERSION     DAZUKZ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.210"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..532
                     /locus_tag="ACG8EF_002850"
     CDS             14..532
                     /locus_tag="ACG8EF_002850"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4948083.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            660..1343
                     /locus_tag="ACG8EF_002851"
     CDS             660..1343
                     /locus_tag="ACG8EF_002851"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFS4948084.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1652..1725
                     /locus_tag="ACG8EF_002852"
     tRNA            1652..1725
                     /locus_tag="ACG8EF_002852"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1686..1688,aa:Arg,seq:tct)
     gene            1884..>2171
                     /locus_tag="ACG8EF_002853"
     CDS             1884..>2171
                     /locus_tag="ACG8EF_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFS4948085.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSL"
BASE COUNT          728 a          337 c          439 g          667 t
ORIGIN      
        1 gaagttgaaa acgatacctg attacaaaaa aataactttt ataagttcta taacgttgat
       61 tatattttta attcttattc ttctcgtcgt cttggttccc cgaatttttg gtttgactcc
      121 ttatattatt gaggacaaag aaatgagtcc atattatgca caaggaaatc tgatatatgt
      181 acgaccgaaa aatacaaatg agattttagt cggagatatt attacatatt atgaaaattc
      241 aggaaaaaaa atctcgacgc gtcgcgtaat agccgttgaa aacaaccacg aagattttta
      301 tacaaaagct gatacaaaga cttatattga gcctggagta gtaaaaaaga gaaatattat
      361 cggtagtccc atctttcaga ttccatatat tggactagtc ctttcaaaaa cggcatttgc
      421 atggattcag gggttattct ttacgattgc tttttgccta acaggaatca cagcatggaa
      481 tacctttgta gagttgaaaa agaagcggag acgaacacag aaatttttgt aagaatgtta
      541 aaaacataaa tgtattccag agatccaacg actcttctga acatgaaaca cgagaaaaca
      601 ctcatgcagc ttgtagattt agtcagttgt tcgtataatg tgtgaagagg aggagtagta
      661 tgcgcaatga aatgatgcat cgtcctgtag tcaaagaaga actattggat tttatgcgta
      721 cccagcaaaa aaaattaccg ggtgagctag gcaaactgga agaagaagct catgtagcag
      781 aagtaccagt tattccccat gaaacagttg tatttttgaa atttttactt ggacagcttc
      841 agcctgaacg aatcttggag atcggagcag ctatcggttt ttcatcaagt gtcatggcca
      901 cgacaatcgg agaaaacgct catgttacga caattgatcg tttcgatgtc atgatcgaga
      961 aagctaaaaa aaactatgaa cgtctaggac tgacagataa agtgactttg cttgaaggtc
     1021 aagcagcaga tatcttgccg gagttgtctg gtccgtatga ctttattttt atggatagtg
     1081 caaaatcaaa atatattgaa tttttacctg aatgcttacg tttgttaaga aaaggtggcg
     1141 ttttgatggt agatgacatc ttccaaggag gaacgatctt gctcccagat gaagagattc
     1201 cccgtggcaa acgagctatc catcgtaaat taaatgaatt tcttcgtgtt gtgatggatc
     1261 atcccgattt gacatcgaca atcttgccat taggtgatgg tgtgatctta atgacaaagg
     1321 aatcagaaac aatcgagttg tgatacatga ttatggcagc ctgtagataa tcgattgaat
     1381 cgagggctca attcgaactt gatgtaaaag tttctatatt tgtaagtcaa ttaaaggata
     1441 caggaaatct aaataaacag gagtagcatg aagtcaatgt agctcctgtt ttcaattgat
     1501 aaccttagaa cagctgaagg agaatgtctc gttttaaaag attgatttag tagttgggcc
     1561 aaaataattc attatttgct aattaaataa ctatttcgta ttgacagaag caatttaatt
     1621 atctataatt attgatgttc cgctgattct tgtcatagta gctcagctgg atagagcatc
     1681 cgccttctaa gcggacggtc gggggttcga atccctcctg tgacgctaaa aaaccgttag
     1741 aaactgaggt ttctaacggt ttttctttgt tttttgtgcc aatattgttt gttttcaaag
     1801 gcaaataaaa taaaaaaaca tccttatcga gtacaattat aaggagcaaa aaaataaaac
     1861 tcatcagaaa agaggaagaa gatatgacga tactatctag aattttagtg actctagttg
     1921 caattgaatt tttttatatc atgtatatcg aaacggtgag aactgattct gacacgacaa
     1981 gtagggtttt taaaatgagt aaagaagagt taagcagaaa atctgttcaa acattgttta
     2041 agaaccaagg agtgtacaat ggactattag gagtgggact actttatgga gcttatcttt
     2101 cttccgcatc taaagaaatc acaagtatgc tattaatatc tatttttttt gttgcattat
     2161 atggcagttt g
//
LOCUS       DAZUKZ010000205         2117 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000205 DAZUKZ010000000
VERSION     DAZUKZ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.211"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG8EF_002854"
     CDS             complement(<1..914)
                     /locus_tag="ACG8EF_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFS4948086.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG8EF_002855"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG8EF_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFS4948087.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          715 a          374 c          411 g          617 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaa
//
LOCUS       DAZUKZ010000206         2074 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000206 DAZUKZ010000000
VERSION     DAZUKZ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.213"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..466
                     /locus_tag="ACG8EF_002856"
     CDS             <1..466
                     /locus_tag="ACG8EF_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948088.1"
                     /translation="ILVYFQGGNLMIKKLFLLSILTLAMAGCSDSGLATELITKSTEA
                     TTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCC
                     ASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(527..892)
                     /locus_tag="ACG8EF_002857"
     CDS             complement(527..892)
                     /locus_tag="ACG8EF_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFS4948089.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(968..1213)
                     /locus_tag="ACG8EF_002858"
     CDS             complement(968..1213)
                     /locus_tag="ACG8EF_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4948090.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1283..1537)
                     /locus_tag="ACG8EF_002859"
     CDS             complement(1283..1537)
                     /locus_tag="ACG8EF_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948091.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1701..2024)
                     /locus_tag="ACG8EF_002860"
     CDS             complement(1701..2024)
                     /locus_tag="ACG8EF_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948092.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          772 a          357 c          365 g          580 t
ORIGIN      
        1 tattttagtc tattttcagg gggggaacct tatgataaaa aaactatttt tactatcaat
       61 attaacgcta gcaatggcag gttgttctga ctctggctta gcaactgaac tgatcacaaa
      121 atcaactgaa gccacgacag aggagttaaa atgtggaaac gatcacagtg actacagagg
      181 cttaaatcga cgatgcatgg gaatattagg cagaagcaat tgtaagcatc gatgtagtaa
      241 tcgaagaagg tgtagaagaa gcaacagaaa aatcaaaaga aagatcaaaa gaagcaatct
      301 gtgctgtgca tcgtatacag acgtcgaaga tgcggtactt ttaaaccacg tcaggagatg
      361 ttcatttatg tctagggaag ttggcgatga accaaaacta gagtacgctt ttagttttgc
      421 acgtgaggat gaagataacc ctaaaactta cgacatttta gcttgatcta agatggttaa
      481 agctttttaa tgaagtaaga ggagatagaa ggcttttcta gttatattag gtaagataaa
      541 ttcctttttg ctccaaattt gctaaacatt ctgctagata aacttcatcg tgttccggat
      601 aaatcggcac gttgcgctct acagtaggta aatcaaaaaa tgcttcgcat gatttgccac
      661 gatacatagg atttttccac tctggtgctg ggctgtaacc acgtttttcc atttcatcca
      721 tgacaagctg atgatattgg aataatctat aaggagaata gtgaaaaaca taatccacag
      781 tagcatgttt ctttccccag ccatttcctc ttaatgcaca gcattcccga tgttgtccta
      841 aaagctgttg acgaggaagc tttgaaatca atttttcatg ccataatcgc atagaaatgt
      901 ctcctttata tatagattgt taaccttaga cgttaaatga aaaaaccagc accaaggctg
      961 gaaaaaatca cttcagtttg cttgctgttt ctgagcgacc aattaggtaa tcaacggaaa
     1021 tatcgtaaaa gtcagcgata tagattaatt caatgatgct tggaatttct tctccttctt
     1081 cgtacttacg atatttgaat aaaggaatac ttaacgcgct agaaacagct acttgagata
     1141 aagatcggcc agttcttaag aaattcagac gacaacgtaa agtatccagt aaaatagctt
     1201 cttcgatact catttaatca cctttcatta tagtagaata gttataatga ctatagaaag
     1261 ctacacatgc aatcaatagt actcaatgtt ttttatttaa acgtatcaaa taaagaatga
     1321 cgatcaataa agacaaaacc agtcctgata attcttctat aaaaacacta tcgggttcaa
     1381 agtagttgta attaaaaact ttccacataa agtagatagc cggaagagtc aaaatagcta
     1441 aataaaaccg ttctttgttc caattcataa gataaacaat acttgcgacc agtgcgaccg
     1501 caggcaccca aaacaaaact ctcaccaacg taatcatgta acggactcct caacgattca
     1561 tataataaaa gataacatgt tattgcatac atcacaaatg aaatgctagg aaaataaaag
     1621 aagaatagat caatgaaaaa tgaaaaagca gaacagtttt ctgctgataa gcaggaactg
     1681 ttccgctgaa tgtgtcttat ctatcgagaa aaaatcaatg gattgatatc taagtaattc
     1741 aatacatata aaaagaggac gatcgcattc actaaaaagg aaagaatgat gatcccatag
     1801 cgaatggaaa catatttaac aaatactttt gtttgaatac gggcatatct ttttccttta
     1861 tagctgagca tcatgacaag aagcaaaagg gtaatcacac cttgaaaaat cattccaata
     1921 aacagaattc ctgaggtttg ataatttaat tggagaaaat aagctgctgc cgcaactaaa
     1981 tcgaataaaa tcgcaataaa tggcaaaata atcactccta acattgagaa taaataaaag
     2041 aataacattt tttctaaaat aagcaaataa aaat
//
LOCUS       DAZUKZ010000207         2069 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000207 DAZUKZ010000000
VERSION     DAZUKZ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.214"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>2069
                     /gene="acm"
                     /locus_tag="ACG8EF_002861"
     CDS             <1..>2069
                     /gene="acm"
                     /locus_tag="ACG8EF_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFS4948093.1"
                     /translation="NITSLIPVHVYADAGRDISSNVTSLTVDPTNITDGGNIKVKFSF
                     DEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNEN
                     IKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYY
                     KTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQK
                     VYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQIN
                     FENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPN
                     VTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSN
                     ELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPV
                     PDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVV
                     TNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTN
                     LPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEK
                     TEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSF
                     AVIIGLL"
BASE COUNT          812 a          341 c          405 g          511 t
ORIGIN      
        1 taatataaca tcactgattc ctgttcatgt ctatgcggat gcaggcagag atatcagcag
       61 taatgtcact tcgttgaccg tggacccaac gaatattaca gatggaggaa acataaaagt
      121 aaaattctcg tttgacgaaa aaaaacaaaa tatccaacca ggtgattatc tttggatcaa
      181 ttggccaagt gaaggaaata ttcgcggtga aggattccaa aaagaaatcc cattaatgat
      241 tgaaaataaa aatgttggaa ctttaacggt cagaaaagat tctgcgcagg ttgtgtttaa
      301 tgaaaatatt aaaaacctag actcagtaga aggttggggg caatttgaga tacaggccag
      361 aaacgtaacc gataccggtg aagaaaatac gggaccattc accgtgacga gcggtgataa
      421 aacagctact gtaaatgtga caaagccagc ttctggttct tcttctagcg ttttttatta
      481 taaaacagga gacatgctac ctgaagatac taagcacatt cgatggtttt tgaatattaa
      541 caataacggc acttatgttg aacaaccagt taaaatcagt gatgaaatcc aaagtggaca
      601 aagattagat ccaagtacgt ttgaaattaa tcaaatacac ttaggtgaac agaaggttta
      661 tagaggagaa gagggaatcc aacaattttt acaagatttc cccagcgcta catttaattt
      721 ttcagtaact gataattaca ttgaaataac tataccaaaa aactttgtca atcttaggaa
      781 aataatggtg agttataaga cgatcataga aaacccagaa caaataaatt ttgagaatca
      841 ttcggaagct tggtttaaag aatttaataa acctgctgtt gatggagaaa gtttcaatca
      901 tacagttaaa aatatttcag cttctggtgg agtcaacggt acagttagag gcgaattgaa
      961 gattttcaaa tatatcaatg acacagagat tggtatccca aatgtgactt tcgaattaag
     1021 acgagcagat gaacagccta ttcaaggaca gtctagtatc ctgttgactt cgaatgaaca
     1081 gggagaagct agtatcaaag gcctgcaagt aggagactat gttgtaaaag aaaaagaagc
     1141 acccaattgg attgattttg atccactaag ttcaaatgaa ctcaaattct ccataaacga
     1201 aaatgataca gagggagtca gtttacctat ttataacaag aaaaaggtta cgaacatcac
     1261 agcaactaaa atttggaacg gtggaaccac tccaagaccg agtatttatt ttaaattatt
     1321 cagagcaagt acaaacaatt gggaaccagt tcctgatgca gaaacaaaac gattagacaa
     1381 tggaataact tcagtgactt gggaagatat ccaacagtat gatgatagtg gaaacgaata
     1441 tacgtttaaa gtgcaagaag tagatcaaaa cggaaatgat tatgtccctt caggttatca
     1501 aaaaattgaa aacgggctag tcgttacaaa tgagaataaa gaagtcattt cagtctcagg
     1561 acaaaaaaca tgggaagaca atgagaacca agacggcaaa cgaccgacga cgatcacagt
     1621 aaatctatta gccgacggtc aaccgatcca acacaaagaa gtaagtgaaa aagatgattg
     1681 gcgctatcgt ttcacaaatc tgccaaaata cagagacggg caagaaatca tctatactgt
     1741 aacggaagac aacgtgcccg aatacagtac gacgattgac gggtataaca tcaaaaacag
     1801 ttatacaccg gaaaaaacaa atatatctat accaggtagc catataacac cgtggaaaaa
     1861 ttttccagag aagacagaga gagagaaaat tagaaatcta ttctctcaaa aattgcaaat
     1921 atttgctcat acaggagaat ccagcgaaca aagaagcaat tatattagta agagagctgt
     1981 acctaaacag ataacaaata aatcaaaaag cattcttcct aaaacaagta gtaaaaaatc
     2041 tagttttgct gtaatcattg gtttattga
//
LOCUS       DAZUKZ010000208         2065 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000208 DAZUKZ010000000
VERSION     DAZUKZ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.215"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1008
                     /locus_tag="ACG8EF_002862"
     CDS             <1..1008
                     /locus_tag="ACG8EF_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948094.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="ACG8EF_002863"
     CDS             1025..1174
                     /locus_tag="ACG8EF_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948095.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="ACG8EF_002864"
     CDS             1171..1617
                     /locus_tag="ACG8EF_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948096.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="ACG8EF_002865"
     CDS             1619..1756
                     /locus_tag="ACG8EF_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS4948097.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="ACG8EF_002866"
     CDS             1794..>2065
                     /locus_tag="ACG8EF_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948098.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DAZUKZ010000209         2055 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000209 DAZUKZ010000000
VERSION     DAZUKZ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.216"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..365
                     /locus_tag="ACG8EF_002867"
     CDS             <1..365
                     /locus_tag="ACG8EF_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4948099.1"
                     /translation="CVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNKIR
                     HSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIHSS
                     IHYLTPQEFEELAKEKMA"
     gene            complement(721..1293)
                     /locus_tag="ACG8EF_002868"
     CDS             complement(721..1293)
                     /locus_tag="ACG8EF_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4948100.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1309..1914)
                     /gene="fic"
                     /locus_tag="ACG8EF_002869"
     CDS             complement(1309..1914)
                     /gene="fic"
                     /locus_tag="ACG8EF_002869"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFS4948101.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          638 a          425 c          308 g          684 t
ORIGIN      
        1 attgtgtcat tcaaacattg aataaagcaa aaatacatta tcacattcca gaaggaatga
       61 ttctacacac tgacttgggc agccaataca cagcaagaga agtggaacaa tggcttaaaa
      121 ccaacaaaat aaggcattct tatagtagaa aaggaacacc ttatgataat gctggaatcg
      181 aatctttcca tgcctcattg aaaaaagaag aagtctacac gactagctac tcagattttg
      241 aagaagcaaa tcgagcgcta tttagctaca ttgaaggatt ttataaccga aatcgaatcc
      301 atagctcgat tcactatcta accccacagg aatttgaaga attagcaaaa gaaaaaatgg
      361 cttaaccgtc tctactaaag acaatcaaca ccgtagggat atggagataa aatcaataga
      421 gtcaatagat tatttatcga taataacact aaaaaagcca cgttcctaaa cgtgacttac
      481 gctgaacgat atgtgttgtt tggtagacga acaaatcgtt cattataaat atatgatggt
      541 atcctaatat agtaagcgtg gctagcatac ctttaattac tttacctttt acagttacca
      601 tacacatttt ttgagcagaa atcaagtaaa taaaaaaata ttatatttca caacatgcta
      661 atagactcat atctagaacg cattaagtat ttacctgtct ggttcgctga gacttctttt
      721 ttaactttct tcaattttct tctttattct aaaaagcgta ctgcgactga aaccggttat
      781 agcttcaact tccttatacg atttacctga agtaagcagt tcatatgcgt gcctcttctt
      841 cggtgtgatt gttgctttcg gacgtccttc tctgaaatta ggattattct ttcttgcaaa
      901 catttttcct tcttgggtac gttccacaat catatcacgt tccatttctg ctacactcaa
      961 caacgtccgt gtcaccattc gccccatgga cgtattttca atcgttccta gattcaaaac
     1021 cttgatcgtt acgttatcat cgagtaaagg ttcaatgatc tctaatgctt ctctcgtgtt
     1081 acgagcaaat cgatcaagct tcgtaacaac tatttcatct ccaggcttca gaccagccaa
     1141 cagctgattg aattttggcc gatcccgttt tgtacccgta aatttttctg aaaaaatatt
     1201 cttcttctct acgcagttct caagcaactg ttgaatttgt tcttttgtac tttgagaagc
     1261 ggttgagaca cgtgcatatc catatttcgt catttggtcc acctactttt acaaatcatc
     1321 tatcgtatac tcagaatacc cttcatagaa atagctgaca tcgattcctt tcatatataa
     1381 ctcgcgatcg tcaattttat cagtcagagc attggaaatt agatagcgta tttccaaatc
     1441 gtttatggga cttcgctcca ttgcagaaag atagtctgct ttatcgatta agttccaatc
     1501 tacaactttt tgcaattctt cctttaaaat gagatccagc caaattctag tacttcggcc
     1561 atttccttct cgaaatgggt gagcgatatt catctccaca tatttcttga taatctcttc
     1621 aaaagaagtt tgtggcatct gatcaatatg ctgtaacgaa tgttctaaat acataactgg
     1681 tgcaaatcga aaatttcctt ttgcaatatt gaccgatcgg atttttccgg caaaatcgta
     1741 aatatccgaa aatagaaatt catgaatttc agataaacct ttaaatgtac caacttcgat
     1801 ttcatttatt tttccacaat cgtacaattc tttcgctttt tgtttactga gtttttcttc
     1861 aaccttagcc aactcaactt gattggttaa tcccaattta ttctctaaaa tcatgcgaaa
     1921 tctccttaac tttatgacat aagtttatga aacactctca accccattat atcgcgattt
     1981 tgtttatgtg tcaaaaacta ttaagtttct gacccccgat agatttttca agtgtctcta
     2041 ttttgcacag tctag
//
LOCUS       DAZUKZ010000210         2049 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000210 DAZUKZ010000000
VERSION     DAZUKZ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.217"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            286..693
                     /locus_tag="ACG8EF_002870"
     CDS             286..693
                     /locus_tag="ACG8EF_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948102.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            707..1108
                     /locus_tag="ACG8EF_002871"
     CDS             707..1108
                     /locus_tag="ACG8EF_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948103.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1110..1481
                     /locus_tag="ACG8EF_002872"
     CDS             1110..1481
                     /locus_tag="ACG8EF_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948104.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1517..1819)
                     /locus_tag="ACG8EF_002873"
     CDS             complement(1517..1819)
                     /locus_tag="ACG8EF_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS4948105.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          775 a          277 c          342 g          655 t
ORIGIN      
        1 gaggggaagt acatatagtt gttgatatag ttgctaatat tgttagctaa tttgttagta
       61 aaactgtata cgaatttaga tagtaaaaaa acagtttgtt ccttatatag aggcactttt
      121 actactgatt aaatgtcaga tatgcttaca aatatactta taaatatgct tacaaaatta
      181 gctaatattt ccgttgacaa agctttgtta ctattgttaa actaaattga ctagcaaaag
      241 tgaaacataa atttattaaa taaatgtaaa aattaggaga aatcaatgtc ttatagagta
      301 caattaataa tttcagaaga tgtagaaagt cagcaatttg gcactaatgt tattaataaa
      361 gtaattaatc cagctctttc tattaatgcg cctttaattc ctactgcttt atcattttct
      421 gtaacagctg tggtgtctga gatagaggat acagaaaaga taaaaatagt agaaattgaa
      481 gttttaaaca agaatgaaaa acaaattttt tctacgggtg aagtatcggt aagcttgcct
      541 cctcaagtta acgatataaa ctttaatata aacgccagaa atgtcttggt tgaagaagca
      601 ggagagcatt atgctgttgt taaatttaat ggaactgaga ttggaaggca aatctttgat
      661 atcaaggtca acaaaccagt ggaaaaaaat taaggagacg atacggatgc ttgatatagt
      721 accatcaaat acacatcgga gtggtagctt aataaatttt gctgcgattt ttgcttgcgc
      781 atcggtaatt ctagcatctc cagttgttgt ggatgcaagt tctactccga aaactaaaaa
      841 tgataatcaa cttgtaatta ctacaaaaaa tgagattaac acaatttcaa gtaatgatgg
      901 caaaattttt gatgtgataa atacggtttt aaaaagaaaa gctgaaatca atataaaata
      961 tgatgaagat ttaaacttgt atttctttgt tatcaaaaca acatctgaat tgttttcctc
     1021 agattacgat gttttggata ctctcgataa tgtattatct gactataaat atatggggaa
     1081 atctgtagta gcaactttgg gggaataaaa tgtttgactt taatgaatat ttatctattt
     1141 ccaaacaaat tgaatcagat gataaatata attctaaaga atcttataga agaactgctg
     1201 tttcaagagc atactacagt gcttttaaaa aatctgatga gtatttgaaa gaaaattatg
     1261 atattattta taatggaagt agcggaaaag gaagccacca aaccgtttgg aatttgtttt
     1321 caacagttaa ggaattgaat accttaggaa tacataatag tgggtataga atgttagaga
     1381 aaagaaaatg tgctgactat attcctaatg aaaccattac gaaaacagat atggcgctga
     1441 tgaatcgaga agcagaaaaa ataataaaca aactcaccta atgaatttca agcgagtttg
     1501 tttgttattt ttttatttaa tctttgtatc tttctggatc aacgaaagta tactttatat
     1561 agtcataacg ccgatgatcg ctacgtgcgt ccggcacgtc agtcacgata tcaaacaaaa
     1621 agtatacacc cttcttcatt ctagttttcg cagcaggaat tttgaaatag ttcttattag
     1681 aatagtagag attgattaat aagctatctt cgattgctaa aaagaaaact tctgaattcc
     1741 atactttata aaaatctttg ataaatctat tcgaagggtc aaatttaaac cataattgtg
     1801 tttttccttc catcagcata aaagttcacc tcaaaaagag tatacgaacg aatgttcttt
     1861 ttgtgaaggc ggaatttaat atactgaata atagaaaaca agtacttgtg ccaaattatg
     1921 tgccaaaaaa attcgaattc aacaaaatgt aaacaaatag aaaaccttct atattaagat
     1981 ttcctgcaat aacaaacaca acagaagacg tgtaatagtt agtcaggaac gtacaaataa
     2041 cccctgtat
//
LOCUS       DAZUKZ010000211         2040 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000211 DAZUKZ010000000
VERSION     DAZUKZ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.218"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1872
                     /locus_tag="ACG8EF_002874"
     CDS             <1..1872
                     /locus_tag="ACG8EF_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFS4948106.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          738 a          330 c          416 g          556 t
ORIGIN      
        1 agtgctgaaa atatggaaga aatcaaaaaa atgtttccga aatccacttg gtttggcttt
       61 acaggtacac cgatttttcc tgagaatcgg aaacaagcca aagggcaatt ggcacgtaca
      121 acccatgatc agtatggaga ggtcttgcat acctatacca ttaagaatgc gctagaagat
      181 ggctctgtct taggatttca agtagagcat gaaaacaccg tggagccgac ttcactagag
      241 aataaaatct atcggaaatt aaaagaagta gaaacatatg cagagtattc atcagaacag
      301 atcaatcgta tgattgatca aatggaacct gtcaaaaaag aaagttattt ggacccagct
      361 gtttttgaag cagatgaaca tatccaaaaa gtcattcata aaatgttccg tccagataat
      421 gcctatacaa aatttgattt tcgaaatggc aggccaacga aatctgcaat cttaaccacg
      481 agttccattg acatggccaa aaagtattat cgggccatca aagagatgac gaaagagcct
      541 gattggttaa caaaagagtt tcccaatcag ctcattcgtg aaggacgaac gatggaggat
      601 cctgattttc cacgaatcgc tattacctat tcattagaag aaaatagccg agattcaagt
      661 gcccaacaag aagaaatgca aaaaattatt gaggaatata atcagtatta tgatacttcc
      721 tggtcattag cagacattga gcgttataac ggagacatca ataatcgttt ggcacgaaaa
      781 agagcggaat tcaaacaatt tgggaaacaa atcgatttag cgattgtagt ggatcgtcta
      841 ttaacaggtt ttgacgcccc tacgattcaa accctatttg tggatcgtaa tctagagtat
      901 gcaggattga tccaagcttt ttcacgtacg aatcgtacgt atcctgataa gacaaaagga
      961 ttgattgtga cgtttcgtaa gccagcgaca atggagaaga atgtcgagga tgcgacaaaa
     1021 ctgtattcaa aagcaaaaga agaatcaggt ttgatttatc caagttatca ggaatccaaa
     1081 aaaagattca aacaagcata tggtaagtta aaagaggttt catccatgga atccattgat
     1141 gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt
     1201 gcttatgaat cgttagtgac ttatgacgat tacaatgatg acatggtgaa atctccaact
     1261 ttgaacaatc aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt
     1321 tcattaatag aggaagaacc agaaaaagaa ggagaagatt tttcaacgat cgaattttat
     1381 agtgaaaata ccactaagtt gtatgatatt gattcaactt atattgatca attactagga
     1441 acttatgctg cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg
     1501 aataaaacag aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg
     1561 actttagata gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcgagt
     1621 gatgatttga ttcatgtctt ttccaatgag tggtttgttt ctgagaaaga attacatgct
     1681 tctaatctgc aatatctacc aggtgaagat ccaattccta atatgaaagc aattatcaac
     1741 agtaaggatt acgaaggata taaagccaaa catccagaag cgaagccatt taaataccca
     1801 caagaaatga agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgagaat
     1861 gaattgaggt aaacgagaag agttaagaaa attgtacgat tcaaagatgg tagtcaagtg
     1921 gaagtgtaga aaatagggat agatgtcttt tatttgttca cattttataa cgaagtattt
     1981 aagacagcta ttgaataatg tttcctccac ctttttcatt catttaccgg ttctgttgca
//
LOCUS       DAZUKZ010000212         2035 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000212 DAZUKZ010000000
VERSION     DAZUKZ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.219"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..1501)
                     /locus_tag="ACG8EF_002875"
     CDS             complement(74..1501)
                     /locus_tag="ACG8EF_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFS4948107.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            complement(1494..>2035)
                     /locus_tag="ACG8EF_002876"
     CDS             complement(1494..>2035)
                     /locus_tag="ACG8EF_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFS4948108.1"
                     /translation="KHDYPRINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYH
                     GSQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGG
                     PDLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYS
                     DKLLNRIDKEILKVGNIDE"
BASE COUNT          769 a          326 c          242 g          698 t
ORIGIN      
        1 gtaaaaattt tgataaacat ttaatatatt aatcaaatgt aaaggagtcc aagcaatgaa
       61 agctgtcttc atattaattc tcctttttta aatacaagta agaaatgctt cccaaataat
      121 accagaaaac aattgaaatg acatcgcgct tattgaaaag tacatgagcc tccaccattt
      181 catctataaa aatagatagt ataaaaatta aaacaatacc aagaaacgca taattcactt
      241 tacttctaaa aaatgttttg agtaaactaa agatatttaa cacatagaaa gcatagatac
      301 atataaaagc aattatacca cttgatacaa gtacttgaag aaatccatta tgcatgtttc
      361 catgtgctct ttttagttca ttcacatctg tagtatcaag atctactttt ttcaaaaatt
      421 ttgcatcgct attcccatag aaatcggtct ctccataacc aaataacaaa gttggtctta
      481 taactttaag cgcagctttc caaatcgtaa aacgaccagc tgaaatttcg gagttatcac
      541 tagaatgctg taatattact tctttttctg gaagtggagt tttatctgat tcatttgact
      601 ctataacttc tacttttcta tcaatcgata caggaattgt tgctaaaaaa tccttagcat
      661 aattagtacc aaaagaaaaa tataccaata gaactaataa tatgcttacc atccatttga
      721 tttttacagg tagtacttct ctaatgctct ttctaaattt tgaattggca aacaacaaaa
      781 gaaataatac taaaaaagaa caaattacta cctgggcacc tctagacgaa gaaagtgtaa
      841 tataaaatat ttgtacgata aaattcatca tataaaaaac acgaatcgtt tttgttattt
      901 ttgaaattaa tacactaatt aaacttaaaa taatagaagt atatccaaac atagctccaa
      961 catttggact tgtatagaca ccaaataacc gatgttcaat aaaaccttgt cttgcaagta
     1021 atcctggcgt tcctgttggg acatgataag aaatcaaaaa aataaattga tagatagata
     1081 ttagcgcagc taaaaaaatt atgataatga aaacattatt aaaatacacc atttttttct
     1141 ttttttcctc ttcactttgt gaaaagtctg caggaaaaat tactatcaaa gtaatcagca
     1201 aatatccaaa attataaata gatggaatta aaaaattttc tctgttaatg attatagtta
     1261 agataaaact aatcattgct ccaatcagta gccaagaaaa acgcatttta aaaaaatttt
     1321 tctttataaa aacctcatac aaaactataa ttccacccca agcaaaagct attttcatcg
     1381 ggatattagt tacattaaaa gtactaggca taaaataaag cagacaaatg attaaattta
     1441 ttactcgaaa cgcatctcga cttaataaac tcttctctaa aacaggtact attctattca
     1501 tctatatttc caactttcaa aatttcttta tcaattctat ttaatagttt atcagagtaa
     1561 tccgatttaa cttttcttac aaataatttt tcactttctt ttatttgatc tatatccctt
     1621 tcatcaaacc atttgttcat ggaaggatca ataatatgaa tatttgctaa tttcgaagtt
     1681 tgtaaagtag tttcttcaat atttttcaat tcaagtggta aatctggtcc tccaataaca
     1741 tttttatttt tgaacgagga attaaaaaaa agtgtatgaa aaaatttttc atctggggca
     1801 aacataccta ttttaaaatt caggtagtct tgttgatttt gttcatacat ttgtattaat
     1861 tctgttaggc attctttcga caaagcccac cattgagaac catgatacaa atcaaattta
     1921 tggtgtctaa tttttggggg aagtttaatc atcctcagac aacgattaat tatagctcta
     1981 aaaattttaa atgcagttgt gttattccta ttaattctag gataatcatg ttttt
//
LOCUS       DAZUKZ010000213         2004 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000213 DAZUKZ010000000
VERSION     DAZUKZ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.220"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACG8EF_002877"
     CDS             <1..378
                     /locus_tag="ACG8EF_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS4948109.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACG8EF_002878"
     CDS             426..1826
                     /locus_tag="ACG8EF_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS4948110.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          340 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt ttac
//
LOCUS       DAZUKZ010000214         2002 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000214 DAZUKZ010000000
VERSION     DAZUKZ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.221"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..618
                     /locus_tag="ACG8EF_002879"
     CDS             <1..618
                     /locus_tag="ACG8EF_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFS4948111.1"
                     /translation="AGGHAKVIIETLRLENKLIYGFFDDKQNKMQDLKYLGKINELNS
                     WADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIAVHPSAIVAKEISIGYGTVIF
                     ANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPGVCLAGGVHVGVGTQIGIGSQ
                     CIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKKEGINFEF"
     gene            569..1750
                     /locus_tag="ACG8EF_002880"
     CDS             569..1750
                     /locus_tag="ACG8EF_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFS4948112.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            1763..>2002
                     /locus_tag="ACG8EF_002881"
     CDS             1763..>2002
                     /locus_tag="ACG8EF_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4948113.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKE"
BASE COUNT          715 a          264 c          415 g          608 t
ORIGIN      
        1 gctggcggac acgcaaaagt cattattgaa accttaagat tagaaaataa gttaatatat
       61 ggattttttg atgataagca aaataaaatg caagatctga aatatcttgg gaaaataaac
      121 gaattgaata gctgggcgga tagcaaaaaa aaaatctata aatttattgt agctattggt
      181 gactgtgtag taagaaaaaa gattataaaa aaattgaata tgccaagtga gcaatatgga
      241 atagctgttc atccaagtgc tatagtggca aaagaaattt caattggtta tggaactgtt
      301 atttttgcga atgctgtaat taacccagat gctgtaattg gagagcatgc tataataaac
      361 acaggtagca ttgttgagca tgattgtaga attaataact atgttcattt atctccaggt
      421 gtatgcttag cgggtggagt acatgtaggt gtaggaactc aaataggaat tggttctcaa
      481 tgtatccaga tgaagaaaat tggatcttgg tgtatgatag gcgcagggag cactattgtt
      541 aaggatattc cctcacattc cttagcttat ggcaatccag ctaaaattaa gaaagaagga
      601 attaattttg aattctagaa ttttattatc aagtcctcat atgggaggaa cagaagaaaa
      661 atatgttgct aaggctttta aagataattg gatagcgcca ttaggtgaaa atgtggataa
      721 atttgaagaa agtattacaa aatatactgg ttctaagggc ggagcttctg cgttaagttc
      781 aggtacagct gccatacatc ttgccttaag actgcttaat gttggaaaaa acgatattgt
      841 ctttgctcct tcatttactt ttatagctac agtaaatcct attttatatc aaggggctgt
      901 accagtgttc attgactcag aacctaatac atggaatatg agccctgttg cactagagag
      961 cgcattagaa gaagctgaaa gaaacaacca attacctaag gcaatcatcg tagtgaattt
     1021 atatggtcaa atggctgatt atgaacgaat tttagctatt gcaaaccaat ttaatgttcc
     1081 agtgatagaa gatgcagcgg aatcgttagg ttctacttat aagggaattt cgtcaggctt
     1141 atttgggaca attgggatct attcatttaa cggtaataag atcatcacaa cctcaggtgg
     1201 aggagctcta gtgtctagtg ataaaaaata tgttgagcat tcaagatttt tagcaacaca
     1261 agcaagagat caagcaccgt attatcaaca tagcgaaata ggttataatt accgtctaag
     1321 caatatttct gccggtatcg gacgtggaca aatggaagta ttgaatgata gagtagctaa
     1381 acgaagagaa attttcaatc gatataagga agctttttct ggaattgata gaattgaaat
     1441 gatgccagaa gtggatggca tggtgccaaa tcgttggttg tctactttaa cgatcaatga
     1501 ttataattct attgatcaag tagttaacgt aataaataaa ctaaatgaaa aaaacattga
     1561 aacaagagct ttgtggaaac cactacattt gcaaccagta tttcaagggg taaaaatgta
     1621 ttaccatgat aataatctta gtgtgtcaga aaagttattt aatagcggct tatgcttacc
     1681 ttctggaagt aatatgacag atagtgaaca agaatttgtt attgatgagt ttaaaaaaat
     1741 attcaagtag gtgtgatatt ttatgaacat aggaattatt ggtttaggtt ccatggggaa
     1801 aagaagactt agactcattc aagaacactt taatgatatt ttactatgtg gtgttgatac
     1861 taatgaggcg agacgaaagg aagttgaaaa ggaatttgga ataccaactt ataatagtct
     1921 aacagaggca aaaaatttgt ttgaaataaa ttcagttttt gtttgtacat cccctataac
     1981 ccatgaattt attataaaag ag
//
LOCUS       DAZUKZ010000215         1991 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000215 DAZUKZ010000000
VERSION     DAZUKZ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.222"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACG8EF_002882"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACG8EF_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS4948114.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACG8EF_002883"
     CDS             852..1040
                     /locus_tag="ACG8EF_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS4948115.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACG8EF_002884"
     CDS             1053..1610
                     /locus_tag="ACG8EF_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4948116.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACG8EF_002885"
     CDS             1642..1884
                     /locus_tag="ACG8EF_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4948117.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          553 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc c
//
LOCUS       DAZUKZ010000216         1968 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000216 DAZUKZ010000000
VERSION     DAZUKZ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.223"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            84..893
                     /locus_tag="ACG8EF_002886"
     CDS             84..893
                     /locus_tag="ACG8EF_002886"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4948118.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            933..1430
                     /locus_tag="ACG8EF_002887"
     CDS             933..1430
                     /locus_tag="ACG8EF_002887"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFS4948119.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1815..1888)
                     /locus_tag="ACG8EF_002888"
     tRNA            complement(1815..1888)
                     /locus_tag="ACG8EF_002888"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1852..1854),aa:Ile,seq:cat)
BASE COUNT          689 a          349 c          363 g          567 t
ORIGIN      
        1 aagttagttt cattttttta aattctcatt taatcttaag gtaacaggaa aatagatatg
       61 taaaaagaaa gtaggaagat aaaatgaaac tagcagcgat tgatttagac ggtaccttgc
      121 ttgatcgaaa tggaaagata ccagaagaaa atatccaagc acttcggaaa tttgatgaaa
      181 atggcggaat cgtaaccgtc gctaccggaa ggaacagcat ttctgctaaa gatatttttt
      241 ccgaattagg aatcggtggc tatttgattt cttccaatgg ttcactgatt gctgaaatga
      301 aggatggaaa aatggcgaaa gttctcagac gttcaaaaat agatagcgat acactgagaa
      361 aagccttctt ctatgctcaa gaagcgaaaa tatccatcat tgctagccga gaaacccaag
      421 atgatcagct gacattcgat gaatcaaatc tagtcaaaga tgacccttat tatgaacatt
      481 tcaatttaca gaaccattct tttgatcaaa ttttaaatat gttggaagat ccttctctta
      541 gctatcttaa attagcatta acagataaga gtgaagaaaa actacagaag ctacaaagag
      601 atctgcaagc agatggtatc caatcagtat tctctgatcc gcatttctta gaaatcacgc
      661 ctacaggtat cactaaagct cattcacttg tttttctggc ggattacctt ggaatcgaat
      721 cgagtgacat catggctttt ggcgatcaag aaaacgacat tgctatgttg aattacagcg
      781 ggttgagtat tgcgatgggg aatgcacagc gacaagtaaa agaaattgca aaccaagtaa
      841 cactgacgaa tgaagaagct ggtgttgctt atattttaaa aaacttctat taaaaagaat
      901 aaaatgacga tatgaatgta ggaggaaaga aaatgttagt tattattgat atgcaaaatc
      961 atattcttga tccaaacagc gaattttata ttccagagtc agatcaattg gcagaacgta
     1021 ttgctcaaag attagctaaa gcacgccaaa ataatgaata tattctcttc acacgggata
     1081 taccagttga actgaaggac aaagaagaag aacgagaaga tttgcaattg atccctcttc
     1141 tttctccaca agaaaatgaa cgagtgatta aaaagtatta ctttacgatt ccacctgaaa
     1201 ctttagctga aatccaggaa actttatttg aaagcaaaga agaacaaaaa gagatcgaag
     1261 tagtcgggat tgaaacaaac ttgtgtgtac tctctaatct gataggcctg cagagtgctt
     1321 ttcctgaagc agactttttt gttgatccaa tgttagtctc cagtagaaaa cacggagaat
     1381 ccgctttaga gctgttgaaa gatttcaacg tctctatcgt cgaatcataa aaatatctat
     1441 cttcatttac gatatttaac acacaataaa aaaagcacgc agaactgttt tagtcctacg
     1501 tgctttttct atcaaagtag attggctttt ttatgacaca gagtgaaacg gcaggctcac
     1561 gacctctttc ttaaatggtg ttcaatcagc tgaccctcgg tattagctta acaaacggca
     1621 aaatatgagg aactattttt ccgtttgtta agctaatact caggtcaaaa cgctgatttc
     1681 acaacctctt ccttaaacgg tattcaaaag cttgcatctg cagtaataag aacagccaag
     1741 taaaaacata agaagctgtt tttacttggc tgttcttatt acttgatgca gggcagcttt
     1801 ttcacaacct cttatgtacc ttgtaggact cgaacctacg accggacggt tatgagccgt
     1861 ctgctctaac caactgagct aaaggtacat cataaataaa atttattttt gttacttttc
     1921 aactatagca cgaatgacaa actctcacaa gtttcaaaat ataagtaa
//
LOCUS       DAZUKZ010000217         1914 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000217 DAZUKZ010000000
VERSION     DAZUKZ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.224"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..122
                     /locus_tag="ACG8EF_002889"
     CDS             <1..122
                     /locus_tag="ACG8EF_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS4948120.1"
                     /translation="ANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            123..875
                     /locus_tag="ACG8EF_002890"
     CDS             123..875
                     /locus_tag="ACG8EF_002890"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFS4948121.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            880..1827
                     /locus_tag="ACG8EF_002891"
     CDS             880..1827
                     /locus_tag="ACG8EF_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS4948122.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          664 a          260 c          383 g          607 t
ORIGIN      
        1 ctgctaatga tatgtatgag aaggagttga aatatttctt tgaaaacgta gtaacaggca
       61 aagaaaactg gaatgattta tatcatgcaa acgaagtatt gaaaatagct gaggagaaat
      121 aaatggaaat tttatttact atttgtggta gagctgggtc taaggggttt aaaaataaaa
      181 atttgaaata tttggcagga aagcccctag tccatttcgc tttagcaatg atagatatct
      241 atcaacaaaa acatcctgaa cataattgca cgacttgttt gaatacggac agtaatgagt
      301 tagtagaatt gacgactaag ttaaatccgt ctgtgaaaat tgtagaacgt aaacctgaat
      361 tagctacaga tactgtaggg aaaattgaag tgatacgtga ttcattctta caactagaaa
      421 gagaagataa tatatttgat ttaattatag atttagattt gacttcacct ttacgtgaag
      481 tttctaatct tgaagaatta atagatgaac atctaaaaaa tttagataaa gacgtctact
      541 tttcagttgt ttctgcaaga cgtaatccct attttaatat ggtaaaaaga attgaaaatg
      601 aagttactat agttaataaa tcaagataca catctaggca acagacacct gaagtttttg
      661 aaatgaatgc ctcaatgtat tcttttaaac ctgaatttct aaaaaaaagt aattatattt
      721 ttgatgggca atgtgggata ataaaaatga gagattatct aattttagat attgatagtg
      781 aagaagattt taaatggtta gaatttttgt ttcctaaatt tttagaagat tcttcggagc
      841 ttcaagatat ctataattat ttatatcgga ggtaaaagga tgagctgtta tcttatcacc
      901 ggtggggctg gcttcattgg ttctacctta gccaatcatt taggagaaga acatgtggta
      961 atcgtcgtcg atgacttatc aatgggaaaa gtagaaaatc ttgatatgac gaaaaatatc
     1021 acttttattg agggagatgt cgctgagaaa tccttgatgg aagagatcat gaaagctaat
     1081 aaatttgatt atatctttca tttagcagca gttgctagtg tggcagattc agttgcccga
     1141 ccagtagaaa cacatcgtgt gaattttgaa agtgtattaa tgattttaga attgatccgc
     1201 aagtatcaac ctgatttaaa acggatcgtg tttagttctt cagcggctgt ttatggggat
     1261 gaaccgactt taccaaaaaa agaagagtcc gtgattcgcc cattgacgcc ttatgcggtt
     1321 gataaattcg ctgcagaaca atatgtgcta gattattgtc atttatatga tgtacccggt
     1381 agcgcagttc gttttttcaa tgtctatggt ccaaatcaaa atccgaattc tccatacagt
     1441 ggtgtgattt ctattctcgt agatcgctat aagaaacaaa tcgtaggaga agcaacttca
     1501 tttacattat ttggtgatgg aagccagtct agagactttg tttatataga tgatgtaatc
     1561 caagcattac ttttagttgc gaatgaggaa gctgcactgg ggaaacaatt caatgttgga
     1621 acgggtaaag caaccacact tttggaattg attcatgcaa tcaatgaaat tctcggtgta
     1681 gaattgacac ttgaatacaa agaagagcgt tcgggtgata tccatgactc tctagcagat
     1741 attactaaga tccagtcaat tggctatcaa ccgaaatatg gtattttaag tggaatgact
     1801 aattatttga aaactgaaat caattaaaac atactaagat aagattagag ggtaaattca
     1861 ggctctttgt caataaggac tgatgagttt tgtaaaatca aatctgggat agaa
//
LOCUS       DAZUKZ010000218         1855 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000218 DAZUKZ010000000
VERSION     DAZUKZ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.225"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..292)
                     /locus_tag="ACG8EF_002892"
     CDS             complement(<1..292)
                     /locus_tag="ACG8EF_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4948123.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNIN"
     gene            complement(289..942)
                     /locus_tag="ACG8EF_002893"
     CDS             complement(289..942)
                     /locus_tag="ACG8EF_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4948124.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1230..>1855
                     /locus_tag="ACG8EF_002894"
     CDS             1230..>1855
                     /locus_tag="ACG8EF_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFS4948125.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          585 a          332 c          244 g          694 t
ORIGIN      
        1 catttatatt atctttgatt tgtatttcat tcttattatt tttttctttt atgaatactt
       61 ttatttcact actcttggaa taaaactcca aagtttgttc tatatatttt atttcttttc
      121 cattcatatt actagaaatt tcatttgcca tattatgaat cttttctttt ctcgtctcca
      181 aatatgtttt aggaaagata aaaaaaatta atgaatgaac taatataatt attattccaa
      241 gaacagaaaa tatttgtatg aacatttttg gaaatatctt taattttttc attttctctc
      301 caatttatat cctacatttc ttatagtagt tatacagtct aattgtaatt tttttctaag
      361 ttcttttata tatacatcta ttactcgatc ataaggagtt tcttcgctat ctttccatac
      421 ataatcaata atttgcattc ttgttaacac ttgtccatta ttatccaata aacattttaa
      481 tacttctagt tcttttgcat ttacatctat ttcttcacca tttattttag ctgtatagct
      541 attaaaatta accgatagat ccttatattc aaacttctct aaatgtccat aattcttctt
      601 aattaaagaa tctattctag cttttaagac cggcaatgaa aatggctttt ctacatatcc
      661 gtctactaaa ttagtaaatg catcaatttt atattcttca tcactaaatg cagtcaagat
      721 tagaattggt aaattgcttt tctttctaat ttctttcaac acttctaaac catttataaa
      781 aggtatctga atgtctaaga taaccaaatt tatatcgcta ttaaattttg acaaggcttc
      841 tcttccatct ttagcttgaa taacagtata tccaaattct gaaagatatt cacttattcc
      901 ctctcttatc atattatcat cttcaacagt taatattttc atatatacct ccacttaagc
      961 ctatacacta gatattatac agcatattat ggctctataa tatctagtgt tggtgacacc
     1021 ttctgttgcc aatacttttt cttttttcta ataaatgcaa acagacacct tgaaatcctt
     1081 tagaataaag ggttctataa tattttgtgt tgttggaaaa tcgaaggcga ttttctacta
     1141 acactttttc ttcgtttcaa ctcaaaaaga gactcacttt tggtaaaatt tagtcgacca
     1201 aaacatcacc ctaccaaagg agtcgctcta tggataatca tactagaaaa ttactcggat
     1261 taacagataa acatttattt tttgatgaag aatggctgat tgaaaaagaa tataaaggcg
     1321 ttcaagcgca gtttattaaa gggagattgg atgactcacc ttcccattgt gaacactgtg
     1381 gatcgattcg tattatccgc aacggttcct atacgacacg tactcagata ttgaaagtca
     1441 aagaaaagtt gactatttta gaattaaaac gtacccgttt tctatgctat gattgtgggc
     1501 agaccttttc tgcgaaaacg gacttagttg atgaacatca tcaactaaca aaggaactga
     1561 agcaggcgat tctcatagaa ctgtacgaaa atcaatcacg taaactaatc gctaagaagt
     1621 acttcgtctc ggatgggacg gtgacacgga tcttgcgtga agcaacgaag cattaccagc
     1681 cacgtatgaa cttccttccg acggtgttgt gtatggatga gttcaaatca atgaagtctg
     1741 tttctggttc catgagtttt atttgtgttg atgggacaac ccatcagcta tttacgatcc
     1801 ttgaagatcg cagactttac aaactaactc aatacttcct gcgctttcct agaaa
//
LOCUS       DAZUKZ010000219         1833 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000219 DAZUKZ010000000
VERSION     DAZUKZ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.226"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACG8EF_002895"
     CDS             <1..969
                     /locus_tag="ACG8EF_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFS4948126.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="ACG8EF_002896"
     tRNA            1159..1232
                     /locus_tag="ACG8EF_002896"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          605 a          317 c          346 g          565 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaatttt tttgaagagt attatacaag ctaaagcttt tcttcattgc cactcaaatg
     1321 agtggctttt ttatgtatcc ttttatggat taatgaaagg atgtttcaca tagttatact
     1381 tctgtatatt tgaaaagttt tactttgatt tttaaataga aagacatttg ggttaaattg
     1441 tgagataata ataaagaaga gtttaaagcg caccccaaac cacttcccca taagtgtgtt
     1501 acgctttaaa ctcttttata tttgaagcca ttaaaaagca taccatattt ttgaaaaaaa
     1561 gtgagaaaaa aggcttacaa ttggagtggt agttaattag tgacttattt ttgattttat
     1621 agcactgata ctataaaata tagatatcat catattacac aatcttaata ctaacttaaa
     1681 aaatatctcc tttcacaagt atggtgataa aattcgttcc gggctacctt tttaggtagc
     1741 ctactttaat ctttatacct ttctggatca acgaaagtat actttatata gtcataacgc
     1801 cgatgatcgc tacgtgcgtc cggcacgtca gtc
//
LOCUS       DAZUKZ010000220         1796 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000220 DAZUKZ010000000
VERSION     DAZUKZ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.227"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="ACG8EF_002897"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="ACG8EF_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS4948127.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          503 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DAZUKZ010000221         1707 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000221 DAZUKZ010000000
VERSION     DAZUKZ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.228"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..585)
                     /locus_tag="ACG8EF_002898"
     CDS             complement(<1..585)
                     /locus_tag="ACG8EF_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4948128.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIV"
     gene            complement(596..1264)
                     /locus_tag="ACG8EF_002899"
     CDS             complement(596..1264)
                     /locus_tag="ACG8EF_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS4948129.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1394..>1707)
                     /locus_tag="ACG8EF_002900"
     CDS             complement(1394..>1707)
                     /locus_tag="ACG8EF_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4948130.1"
                     /translation="IFENKRIFDLILFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKT
                     IGMINQNLVFNEEEIHFYIYSFYATFYDILKHSYSKEKTMLLTRRLYQFSVPGWIELL
                     S"
BASE COUNT          622 a          283 c          226 g          576 t
ORIGIN      
        1 aacaatagta aatcctttat tttttaaatc acaaagtaag tcagcaagtt tcttagtcgc
       61 tttgaagtcc aaatttgaag acggttcatc caatataatt aatttagagt ttagcgataa
      121 actacatcca atagaaattt tctgccgttc accacttgat agcgaaaaaa tgtttttatc
      181 taatatattt gaaagatcta ataattcagc taattcattg attcttttat ccatatgctc
      241 ttttgtaaat ccaaagtttt ccatcgaaaa tactaactca ctcgttgtat ttgttgtaaa
      301 gaattgtcct ctaggatctt gacttacgta gccaatattt tttgatatac tttcttgtgt
      361 taaggattgt agcgagttat tttcaaataa aatttcccca accaattttc cgtcataaag
      421 tcttggaata agtccattca ttaactttaa tagtgtggat ttcccactcc cagaagaacc
      481 agtaatcaat gtaagactgc ctttttttat tgaaaaatta aggtcaacaa gcgcttgttc
      541 cttaacatcc ttatatgaaa aagaaacatt gttaaattca aacaattctt tcacctcaaa
      601 tcaaaatact taagaaaaaa atagtaacgg aaaacaggat gatatagtct aataagctca
      661 tgtttgtttt atagtaagaa cttcttttca caggagattc tatccccttt gttattgcat
      721 tagcggtcat ttcttcagca atcatagttg cacgtataag aacgggaact aacagaaatt
      781 ccaaatattg cagaggctga aaaaaagaca agcgaatgtt tctcattttt aaagattctt
      841 taatcattct tatttcagaa cgataagtcg gaaagaatct gatcactact gtaatactta
      901 acattatttt tttcggtaaa tgtatttttt ctaaagagga taataattca ttgctagtat
      961 agacactaac caacaaccat gagatcatta cgaccggcaa cattcgaaat acaatatata
     1021 aggatgtcgc aaaaaatata gtaaaaaact ctatcttgat agcatcaata attttcatta
     1081 ctaaaaacag aactgaataa atcaagatga aataaataca tgtctttatt ttacgaaaca
     1141 tcaatactaa aactgtagca ataatgaaac accccacttg aaatatattt ccctttccta
     1201 aaaaaataat gaccggaaaa aaaatattta aagtaatcaa ggttaacgga ttgatttttg
     1261 acactctttc acctccaaaa aataagatcg cactgttatc ttaagctgat aaaaatgcac
     1321 cgttattcag ataacagtgc gatcttatca aaaagcataa ctgagaacaa ttctcaatgc
     1381 aagctttttt taattaactc agtaattcaa tccacccagg aacagaaaat tgatacaatc
     1441 ttctggttaa tagcatagtc ttttcttttg aatagctatg cttcaatata tcatagaatg
     1501 tagcgtagaa tgagtaaatg tagaagtgga tttcttcttc attaaagact aaattttgat
     1561 tgatcattcc gatagtcttt aacttatcta gacaacagac tgtctttttc gtcacaaatg
     1621 atgtaatttt ttcaacaata tcttgatatt tggttccagc tgattggaat aaaatcaaat
     1681 cgaatatacg tttattctca aaaatat
//
LOCUS       DAZUKZ010000222         1691 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000222 DAZUKZ010000000
VERSION     DAZUKZ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.229"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..1090
                     /locus_tag="ACG8EF_002901"
     CDS             62..1090
                     /locus_tag="ACG8EF_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFS4948131.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            1146..1364
                     /locus_tag="ACG8EF_002902"
     CDS             1146..1364
                     /locus_tag="ACG8EF_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948132.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1435..>1691
                     /locus_tag="ACG8EF_002903"
     CDS             1435..>1691
                     /locus_tag="ACG8EF_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948133.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTI"
BASE COUNT          638 a          193 c          320 g          540 t
ORIGIN      
        1 ctagataatt ttttggattc aaatgatgaa atattatatt taaataagga gtatgataat
       61 aatgaatagc aaaaaacttc agatgggtga aattgttaga ggtcctagtc aaatatctat
      121 tgatgttaca tataaatgta attttagatg taaacattgt tacaatgcga gtggtggaga
      181 caattgtttt atggataatg agttgactga tgatgaactg ttagaactta caaaagatat
      241 atgtaattta aagccatata atatttgttt ctgtggagga gaaactttac tgagaaaaaa
      301 aatagtttta tcttgtgcca ctttattaaa agaggcaggt attcctaata ttgctattgt
      361 tactaatgga tcattagtaa ctcaggatgt tgcaaaagaa ttattcaaag caggtattaa
      421 tcgatttcaa gtaagtttag atggaaaaaa tgctagtagc catgaagcac ttagattata
      481 tccaaacagt tttgaaaagg caattaatgc tatcaaatta tttagagaat tgtccgaaga
      541 agtggatgtt gctttttgtc ctactagttt taattacaat caattacgag aagtatatca
      601 actagtaaat actcttaaag tgaagtcttt aagaattcaa ccatttatgc ttagtggtag
      661 gggaatgcaa aatagggaaa tagtgccaac tgtttctcaa tatagaaaac tagtttctga
      721 tattaactat attaaaagaa atgaagatta taccaagata gattggggag atccaattga
      781 tcatatcatc cgacacagaa ctttattgaa agatttttcg aattttgttt ctattaggtc
      841 agacggagga ttaatggcat caccttactt gccaattcaa gttggaaata ttaaatgtca
      901 cagtctttct aaatattggc gagcaggact ttataaagtt ttttctttag atttagttca
      961 agatatagct actcaagttt cttcaataca agatatgagt aaagaattaa aaaaaggaca
     1021 tattaaatgg cttaatgaag atatagaaat tgatattctc gatgaataca aacatgatat
     1081 tttaatttag gtttatcatt ggaaagttta gataaacaaa gtacttttag aaaggaggga
     1141 aaaaaatgat ttctgaacct caaatgaata atatgtatga aagcccaact ataatgggag
     1201 ctggtgaagc tgcgccaaga gcttgttcag caattatggt gtgtgtgtta ttagcatctg
     1261 caggagtagg tatccaagca ggagcggtgt atacttatgc tgtagttgct acagtagctg
     1321 tagctgcagc cgcagtttgg aaaacagtag gattagttgg ttaggaatta ataggagact
     1381 aactgtctcc tattgattcc aatgattaag ctaaggagga attttaagaa aataatgaaa
     1441 tacaataatt taaaaccgaa attacttaat gagaacatag aatatataag agaagaaaat
     1501 tatggatatt taaccatagt tcccaaaaat ggaactgaat tgaaagataa cgttttcaat
     1561 ccaacagtaa gatatatatt ggctaactgt actggaaaaa acagtgtaaa aaaaatagcg
     1621 aatggtttaa aaaagaagtt tcctagttta gaatatgaaa aattctatca agatgttgta
     1681 ggaactatta a
//
LOCUS       DAZUKZ010000223         1672 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000223 DAZUKZ010000000
VERSION     DAZUKZ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.230"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..1622
                     /locus_tag="ACG8EF_002904"
     CDS             75..1622
                     /locus_tag="ACG8EF_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFS4948134.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
BASE COUNT          560 a          305 c          343 g          464 t
ORIGIN      
        1 tgaaccccta tttcttgtgc gtttcttgtg gtattctgta acaaaatcag ttcgattcaa
       61 ggtggtgaaa aattttggaa actacagata cgttgcttca gttgcttcaa gaagcccata
      121 aaaccaatca agcacaacaa caaaccattc agaatcttac aactgaaatc caactattga
      181 atgaaaaggt caattattta accaacaaat tatttggacg ctctaaagag actctctttg
      241 aggaaacgaa tgggcaattg aatcttttta gtgatgaaga aatttctgta agtgttccag
      301 aagcagctgc caccattatt ccagtaaaag gacatcaacg agttgtagga acgaaagcag
      361 acaaaatcaa acacttacct ataacagaga aagaacatct ccttcctttg gaagaacaat
      421 tttgtgaaca ttgcggttca cagatgaaag atatcggacg gacaaaagta cgagaagaaa
      481 ttcgtttcca tcaggctatg ttggattgtc tcacccatta tcaacatacc tattgttgta
      541 aaagctgcga aaaagaagga ctttcttctt ttaaaaaagc gatcgttcct aaacctttga
      601 tttctaatag cttaggttcc aatagtttag tggcggaaac gatccggatg aaatttggtc
      661 agaaagttcc tgcctatcgg caagagaact attggaaaca aacacatggg ttggatattt
      721 cgagagataa tatcaccaat tggcatatta aagcagtgca aaacgcgtta gaccctttag
      781 gagaacgatt aagagtctat cttaatcaag aagagattct acacggggat gaaacaagtt
      841 atcgtgtgat tgaaagtgcc aaaacagata cctattactg gcagttttgt acaggaaaag
      901 acagtcagca tcccatcgtt tattatcacc acgacgaaag tcgtgctgga gatgttccga
      961 aaacttttct caaggagttt acagggtatt tacactgtga cggctatagc gggtataatg
     1021 ccgtggaaag tgtacgactt gtctattgtt tcgcgcatgt tcggcggaaa tttttcgaag
     1081 caattccaaa aggaaagaaa aacacggata ttcccgcagc tcaagcagtc aaacaattgg
     1141 ataaatggtt tgtcctagaa aagaaatgga aagacttttc tcctgagaag cgattaagct
     1201 gtcggcaaca agaactgcgt ccattattta ttgcttttta tgagtggatg gcaacgatag
     1261 atcctgttgc aaaatccaag ttggatgcag ccgttcaata tgcgtgtaaa ctacgaagtg
     1321 gttttgaacc tatttttgaa gatggtcgtt tagaactcac aaataatcgt gcggagcgaa
     1381 atatcaaaga gctggtgata ggacggaaaa attggttgca ttccacaagt cttgaaggag
     1441 cacgaacttc aggaatcatt ctgagtgtat ataaaacagc agaacttaac ggattgaatc
     1501 ctgtaaagta tctagagttt ctatttgata agataccaaa tcttcccgta ctttcagcag
     1561 aaactttaga tcaactgttg ccttggaaca aggatgtaca acaacatttt tctcgtaact
     1621 aaaaacgcca aacattcccg attctattct ataggtcggg ttatttgacg ta
//
LOCUS       DAZUKZ010000224         1664 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000224 DAZUKZ010000000
VERSION     DAZUKZ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.231"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG8EF_002905"
     CDS             131..505
                     /locus_tag="ACG8EF_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4948135.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG8EF_002906"
     CDS             complement(619..1248)
                     /locus_tag="ACG8EF_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4948136.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG8EF_002907"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG8EF_002907"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAZUKZ010000225         1650 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000225 DAZUKZ010000000
VERSION     DAZUKZ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.232"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACG8EF_002908"
     CDS             <1..1412
                     /locus_tag="ACG8EF_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS4948137.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
BASE COUNT          654 a          250 c          354 g          392 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa
//
LOCUS       DAZUKZ010000226         1631 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000226 DAZUKZ010000000
VERSION     DAZUKZ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.233"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..1509)
                     /locus_tag="ACG8EF_002909"
     CDS             complement(28..1509)
                     /locus_tag="ACG8EF_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4948138.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          512 a          224 c          285 g          610 t
ORIGIN      
        1 gaataagagg atgtgacaag aattttgtca catcctcttt gttttgttct cataaccttt
       61 tttcatatac agttcatgaa ccgcttttat taacttgcca aataacactg tattgtgcgt
      121 tgtatccaga tagaaagtct ccgtttcatc tgaaattttc tcatgctgat ctgtaatgat
      181 caaatcagca tgttctgaat atgcggtaat tttaacagca tctttgcgaa ataactgctg
      241 caaatggctt tgaagatgat aatttcctgt aaactgtttg gttatctgca aatggatgag
      301 taactgctct ttcgtttctg tttgtagtaa gtgttggatc aattgactaa ggtattgact
      361 gatttggttt ctttccccat tttttgaaaa aattgtatcg attttttctc ttaatttctt
      421 ttcctgtata aaagaactag aatgattgga cgtggcttct ggaaagcaaa gtgtagaaaa
      481 actactgctg gattcaccca acagttcgaa aaaaacaact gccaacacaa gaaaataaaa
      541 ataaattggt attttatttt ctaaatggtt gagaggaaac tgttgagtta agtaagtcat
      601 caattgaccg acttgtcgac aaataaggtt tttgcttgtg gaaaataaaa aaccaatctc
      661 ctgtttttgt cgaaaagaga tttgatttgg aaaaaaacaa tagaaaaaat aattaaagta
      721 ggctctctct gtttctgtct cttcacaagg gcgactcact gaaaaagaag taaagtattc
      781 tgaataatca tgtgtatgct gcaataagtc catcaattca gtgatttcag aagaagtttt
      841 tggaaaatat gcctgttgtt tgtttcgtaa tatgaataaa gttaacagca agtagatatt
      901 tttttcttgt tttgaaattt tttctatttg ctcatctgta ctgttcattt ttagtgcatt
      961 taattcaagt gaggaaaatg gccaatcgtt acctccaaat gtatctgaaa gaaaaaagta
     1021 aagatagaga cggatagata actcatttcc ttcaagagta cagcaatttt ttacagatag
     1081 gatccgtaaa tggaattttt ttaaggaatg attgagctgt tttgtcagtt tttcgagata
     1141 ggagggcgat atataaagtt gttcaagtaa atattttttt ggtacagcag tttgtggata
     1201 attgattagt aacatcaaat agttaaaacg attggattct tttaggtagc ataattttaa
     1261 cttagaaaaa acagctaact ttttagtttg atcggtttct ttggtttgga tctctccttt
     1321 atgattagaa taaataaatt catcaatcaa taaaaattgc ttgagtgata catttatttt
     1381 tcggatcagt tctttgattg tccgttttgg aatatgtaag tcattggata attcttgtaa
     1441 agaacaatta tttttttgaa tgaccatttg tagaacaagt aactcttttt ttcttgcttc
     1501 aattagcata aatacctcct ttttattctg tttttgtata tagttagaca aaaactgtga
     1561 gtggaaagtc atttcagtct gtgttattct cacagagtgt cgccttcttt tttatataac
     1621 gttatttaat t
//
LOCUS       DAZUKZ010000227         1611 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000227 DAZUKZ010000000
VERSION     DAZUKZ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.234"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..1567
                     /locus_tag="ACG8EF_002910"
     CDS             101..1567
                     /locus_tag="ACG8EF_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFS4948139.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          451 a          292 c          334 g          534 t
ORIGIN      
        1 tagaaaatta gtttatattt cttaaataag ttttttagtt aaatttttcg gtaaagatgt
       61 ataatatttt ctgaaaattt aaagaattgg ggtatagatt atggaaaaac agcaagacaa
      121 gggcttcttc gggcagccga agggattgtc aactttgttc ttcactgaga tgtgggaacg
      181 atttagttat tacggtatgc gtgcattact tatctattat atgtacgata cagttgccaa
      241 cgggggactt ggattaccac gagcaacagc attagctatc atgtcgatct acggatcttt
      301 gatctacatg tctagtatcg tcggcggatg ggtatctgac cgagtgttag gagcaaaacg
      361 cactgtattc ttcggtggag ttttcattat gcttggccac atcgtcttgg ctttaccatt
      421 tggtgtaagt acattgtttg tttcgatggc attgattatt atcggaaccg cctttttgaa
      481 accaaatgtt tctggcatgg tcggacatct ttattcaaag actgacttgc gaagagatgc
      541 aggattttcg attttttaca tgggtgtcaa cttaggggca ttattagcac cactaatcgt
      601 tggtacaatc ggtcaagaat ataactatca tttagggttc tctttagctg ctattgggat
      661 gttctttgga ttattacaat acatcatcca aggacgcaag acattagacg gagtgggtac
      721 aacaccgccg aatcctttga caactgaaga acgtaaagga ttcatccgta acttgatcat
      781 cgcacttgtt gttatcgctg cagtattcgg cggggcacaa ctaactggtc atctgacaat
      841 tgactttttt gtcaatacaa tcagtgtatt ggggattatc ttaccgttgt attactttat
      901 caaaatgctg acttcaaaag atgtaactgc tgaagaaaaa ccaagagtat gggcttatat
      961 cccattattc cttgcagcaa tcattttctg gtcattacaa gaacaaggtt catctatctt
     1021 agctttattt gctagtgaac gtacacaatc tacgctattt ggtgtaacga tccaagcaag
     1081 ctggtatcaa tcattgaatc caattttcat ctttattctg acaccagtat tcgtaacgat
     1141 gtggacgaaa cttggtgaac gtcaaccatc tacagtagtg aaattctctc ttggactttt
     1201 atttgcagga ttatcgtact tgctattgat gttgccgggt atgctttatg gtacaagtgg
     1261 aaaagtaagt ccattgtggt tgttgggaag tttcttcatc gtggttattg ctgaattgtg
     1321 tctttcacca gttggtttgt ctgtgacaac aaaattagca ccacgagcat ttgaatcaca
     1381 aacaatcgct atttggttct tagctgatgc ttcttcacaa gcaatcaatg cacaaattgc
     1441 acgcttctac acaccgggaa cagaatctgc ttacttcggt attatcggtg cagtagcaat
     1501 cgtcggtggt atcttgctat ttactgtgaa agaaccaatc aaaaaactaa tgggctctat
     1561 tcattaatca atgaacataa aaacaggaat tgcttatttg cagcagttcc t
//
LOCUS       DAZUKZ010000228         1602 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000228 DAZUKZ010000000
VERSION     DAZUKZ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.235"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACG8EF_002911"
     CDS             316..570
                     /locus_tag="ACG8EF_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4948140.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACG8EF_002912"
     CDS             571..888
                     /locus_tag="ACG8EF_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4948141.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          565 a          248 c          306 g          483 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 gacaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaacctt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacttc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 aacccgtggt gctagttaat tttaatatcc taaagaatat ctcaaagttc cttcaaaacg
     1561 tcgttgtgct cgacttaata gaacactgta agccagtaat at
//
LOCUS       DAZUKZ010000229         1521 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000229 DAZUKZ010000000
VERSION     DAZUKZ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.236"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..1200
                     /locus_tag="ACG8EF_002913"
     CDS             31..1200
                     /locus_tag="ACG8EF_002913"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4948142.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          550 a          270 c          244 g          457 t
ORIGIN      
        1 taaaaaagaa aggagactaa ataaactatt atgaaaaaag gaaaacatgc taaaaaaaga
       61 aaatattcat ttttaatgat cgttgtcgtg ttgtttagcc ttagtgcgtt ctctacagga
      121 actattcttg gatggcaatt ttacaataat gaacactctc agatagttgc ccatgctcaa
      181 tcagctcaaa aaaagttgat actgactacc aatgaacaac agctcgttga aaaaatcgat
      241 caactattaa gaaaaaatga ttatattggc tcaatttacg taaagaaaaa taatcgcatc
      301 atcatggaaa aaggctatgg ctatgcgaat aaactcacgc aacaaccaaa tgatccttct
      361 ctttattatc agattggttc ggttcaaaaa gcattgacta ctcttttgat tttaaaacaa
      421 gtcgagcgtg ggcacctttc tttagatact aagctggctc aattctatcc acaaattgct
      481 ggcagccagc aaattactat ccgtgacttg ctgtatatgc gctcaggctt gcaccgaact
      541 gcttctccaa ccataccgat gactgatgaa gaagtcgttc aatttgcgat tcagcactta
      601 aaatttacag attatcagac ctctcattat gattcattga actttaccat attgactgga
      661 attttgatca aattaacaaa tcaaccgtat gaaaaattat tgaccgatga tatcatcgaa
      721 cctttaggat tacagcatac aaaattttat gaccaagtca aaaattcatc caatcatgca
      781 ctatcctatc aaatgtctaa agaggatgat tactttaaac cactgacaga atctgaaaca
      841 gagatccata acgaattagg gactggtaat atcagtatgt ccgtctatga tttaaatacg
      901 ctgtttacac aagttttatc tggaaaacta gtttccaaag acttactctt ctcattatgg
      961 cgtgaaaatg cgaatggaca tccatatagt ggtggggtat atagcggaga taattatatc
     1021 ctgtcgcaag gaaatatcga ctcttttgat acatctgcta tatttagcaa agattcacag
     1081 gatgctatca tcatggaaag caatataaaa gctgacaaaa ctatcaaact gcctactgct
     1141 gatctaagaa accaaatcta tgacctgatg gaaggaacta ctaagttggc aaatagctga
     1201 tatgatgaaa agatagcaaa tactacttct ttgaatagca tttgcttaaa aaaagtactc
     1261 gactaaacgt aggaaagagt gtgggacaaa tctaaaaatc aatttttgct acagacttta
     1321 attccaaata aacagaacag aaagaggtgt tcttctttct gttctgtcca aattattcta
     1381 agatttctct ttaccaacat tatttgacaa agggcctatt aaatctaaaa tagtatcttt
     1441 agattgctaa aatatgtgct agcaaatcat tcatggcttg ttcttatttt taattttatg
     1501 caataaaaaa gaagaacaat t
//
LOCUS       DAZUKZ010000230         1483 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000230 DAZUKZ010000000
VERSION     DAZUKZ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.238"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            176..1264
                     /locus_tag="ACG8EF_002914"
     CDS             176..1264
                     /locus_tag="ACG8EF_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HFS4948143.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            1261..>1483
                     /locus_tag="ACG8EF_002915"
     CDS             1261..>1483
                     /locus_tag="ACG8EF_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4948144.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKD"
BASE COUNT          573 a          172 c          247 g          491 t
ORIGIN      
        1 atacgctcac ttatccagac actcaaatga tttttctctt agtacctatg atagtttcgt
       61 gggttgataa tcaacaacga atatcggaat agatagctaa agtatatttt aattatgaat
      121 ttataattag ttgtaaaagt taatatttaa cggacaggaa gtagggtgaa aaaatttgaa
      181 aaaaatttta gtgttagcaa atacatatta tcaaataata gtagcaatac aaatgaaaaa
      241 tacaattttt tccgatgagt atgttgaaat tattatttct gatcattcta aaaacactaa
      301 agaaattgcg aaaaaaatag aagaaaaaaa agtgtttgat ggctgtcatt atttagaaag
      361 caaatctttt gatgataata ttgggtattt tcaaaagatt aaggactgtc ttcagatttc
      421 ttgtggtaaa tcaaatcgat atgcctatta tttgaaaaac tgtaaaagtg aatattttga
      481 tgaatttatt tgtttttcaa tgggagaggt ttggttggaa ggaatatatg cttgcttata
      541 tcgaaataat aaaaatgtac aggtatcaat tttagaagaa ggtattttaa gctatggagt
      601 tgacattatt ccaacagcaa gaacaaaagc tatcgcagta ctcaattact taaaaaaaag
      661 gactccacta tataacaaaa gctataattt ttattgtttt tttcctaata tatatacagg
      721 aagagagaat acaatacaaa ttccattagt tacagctggg tcaagaaccg cattgcaact
      781 ttcggatatt tttaacccta tgatagatat ttataaagaa aaaaaatata tattttttac
      841 gtctgtttat gattttgagg gcggagaacc tgtcggagaa tatgaattag tatgcaaaat
      901 agcagactta gtaggaaaag ataatctaat tgttaagacg catcctagag atgtaagaac
      961 aatttataaa gataatggat ttatcgagga taaaaattca tcaatacctt gggaggcgat
     1021 tcaattgtca ggcgatttta acgataaaat atttatgaca attaattcag gaagtgttat
     1081 ttcaggtagt acaatgtctg caaaacctgt acgcacgtat tatatgtata aattgtgtaa
     1141 catagaagga aatccgtctt gcaaaaagaa tgcgtatgat attgagcgat tgcttaagca
     1201 aaaaacgatg aaaaacattt taaagaatgt gaaaatagca gaaagcttag atgatatact
     1261 atgagcaaaa ctaaatattt aattaaaaat atgggaatat taacaataag taatttttct
     1321 tcaaaaattt tagttttttt gttagtccct ttatatacaa gtgtattatc cacaacagaa
     1381 tatggcacat atgatttgac aatttcaaca gccacattat tgtttccaat tttaacctta
     1441 aacattgtag atgctgtgat gcgcttttta atggataaag ata
//
LOCUS       DAZUKZ010000231         1451 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000231 DAZUKZ010000000
VERSION     DAZUKZ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.239"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..625)
                     /locus_tag="ACG8EF_002916"
     CDS             complement(<1..625)
                     /locus_tag="ACG8EF_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFS4948145.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="ACG8EF_002917"
     CDS             complement(784..1050)
                     /locus_tag="ACG8EF_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4948146.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="ACG8EF_002918"
     CDS             complement(1040..1396)
                     /locus_tag="ACG8EF_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4948147.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          405 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg a
//
LOCUS       DAZUKZ010000232         1441 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000232 DAZUKZ010000000
VERSION     DAZUKZ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.240"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..428)
                     /locus_tag="ACG8EF_002919"
     CDS             complement(138..428)
                     /locus_tag="ACG8EF_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948148.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACG8EF_002920"
     CDS             complement(546..806)
                     /locus_tag="ACG8EF_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948149.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACG8EF_002921"
     CDS             complement(943..1356)
                     /locus_tag="ACG8EF_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFS4948150.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          530 a          237 c          213 g          461 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 t
//
LOCUS       DAZUKZ010000233         1431 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000233 DAZUKZ010000000
VERSION     DAZUKZ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.241"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..370)
                     /locus_tag="ACG8EF_002922"
     CDS             complement(<1..370)
                     /locus_tag="ACG8EF_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948151.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGL"
     gene            complement(387..>1431)
                     /locus_tag="ACG8EF_002923"
     CDS             complement(387..>1431)
                     /locus_tag="ACG8EF_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFS4948152.1"
                     /translation="KTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLK
                     MNNLNVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTEN
                     LSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSV
                     SGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRD
                     SGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFK
                     NEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQ
                     YQLGLVDNFNNIS"
BASE COUNT          501 a          242 c          159 g          529 t
ORIGIN      
        1 ttagacccac gtaataatct tcccagcttt ctctttcaat taactgatcc aatttttgat
       61 aatccataaa ttcatcatgt atcaaaaaga ataaataact tgatatttta gcatctagct
      121 taatcagatt aaataatgtt tgtttaaaat tgctaccatt aatatgactg atagattctc
      181 tagcttcttc acataaactt tttactttat taattactac tttgtcatat tcatttaaca
      241 cactattatt accattactt agacgctcag attctacgaa acaactcaaa tcagaaattt
      301 cccaaaaaaa ttttttgtta aaatccaatt tcctcaactc ctccaaacta cgtaaatgat
      361 atagtctcat aataaaataa aacctctcag ctaatgttgt tgaaattgtc cactaaacct
      421 aattgatatt gcttaggaga tacacctgcg tactttttga atactctcga aaagtgtgaa
      481 gaatctgaaa atcctaaacc taaagaaact tcactgatag tatggtttga tttcaacatt
      541 attttagccg tagaaatttt tcgcttagag atataattat aaatcgtaat atgcatttca
      601 ttcttaaagc tttctcttaa tttagtttca cttacagaaa aataattagc tatttccttt
      661 acagtaattc ttttatagat atttaagccg atgaattgaa cgacagaaga aattaaggga
      721 gaaagatgct ctatcttagt ttttccgatt tccttagtat aataaacaat cccagaatcc
      781 ctcactttta agacctcagg taacgataca gctaattcgt tttcttgtat caaagcatct
      841 ctactttgat acgcttctat tatatccatt cctaatgtta tagacgtttg tgctaacttt
      901 tcaaaaacaa tgatagaata atttttttca gaccgaatcg tatctccact aacacttgga
      961 attattccat tactcagatt aaaaaccatg ttttctaaat ttcgaatatc tccattttta
     1021 acatattcca gtattttatt ttcataaaga aacagatata tttctggatc gtaattttgt
     1081 gataacagat tatctatttt gatttgctcg atagttttac ttagattttc tgtatattca
     1141 attaacggtt catggaatag atcttctatt tttccagtaa aaaaataatg aactaaaatc
     1201 agaatatctc gaatgtctcc taaagaatac aaagtcaaat tttgcaatga attatagagt
     1261 cgttctctat cagccattgc aacattcaaa ttgttcattt ttagtttaaa aaaaacttta
     1321 tcaatattat ttgctctaaa tggaccaaat aagtaatatc tggttttaaa catgtataga
     1381 agaaacatct cattaaaatc accagtaaag aaatggaaat ctttagtttt t
//
LOCUS       DAZUKZ010000234         1417 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000234 DAZUKZ010000000
VERSION     DAZUKZ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.243"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8EF_002924"
     CDS             complement(150..1337)
                     /locus_tag="ACG8EF_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFS4948153.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZUKZ010000235         1374 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000235 DAZUKZ010000000
VERSION     DAZUKZ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.244"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1344
                     /locus_tag="ACG8EF_002925"
                     /note="aph(2'')-Ia"
     CDS             <1..1344
                     /locus_tag="ACG8EF_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFS4948154.1"
                     /translation="FILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIY
                     KMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVI
                     LDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYL
                     IEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTN
                     LETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQ
                     MHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEG
                     KKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGED
                     ILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          581 a          108 c          229 g          456 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga accgaatcat taaaaaaaca ttatacagag
       61 ccttgggaag atgaagtttt tagagtaatt attgaatata acaatgttcc tattggatat
      121 ggacaaatat ataaaatgta tgatgagtta tatactgatt atcattatcc aaaaactgat
      181 gagatagtct atggtatgga tcaatttata ggagagccaa attattggag taaaggaatt
      241 ggtacaagat atattaaatt gatttttgaa tttttgaaaa aagaaagaaa tgctaatgca
      301 gttattttag accctcataa aaataatcca agagcaataa gggcatacca aaaatctggt
      361 tttagaatta ttgaagattt gccagaacat gaattacacg agggcaaaaa agaagattgt
      421 tatttaatgg aatatagata tgatgataat gccacaaatg ttaaggcaat gaaatattta
      481 attgagcatt actttgataa tttcaaagta gatagtattg aaataatcgg tagtggttat
      541 gatagtgtgg catatttagt taataatgaa tacattttta aaacaaaatt tagtactaat
      601 aagaaaaaag gttatgcaaa agaaaaagca atatataatt ttttaaatac aaatttagaa
      661 actaatgtaa aaattcctaa tattgaatat tcgtatatta gtgatgaatt atctatacta
      721 ggttataaag aaattaaagg aactttttta acaccagaaa tttattctac tatgtcagaa
      781 gaagaacaaa atttgttaaa acgagatatt gccagttttt taagacaaat gcacggttta
      841 gattatacag atattagtga atgtactatt gataataaac aaaatgtatt agaagagtat
      901 atattgttgc gtgaaactat ttataatgat ttaactgata tagaaaaaga ttatatagaa
      961 agttttatgg aaagactaaa tgcaacaaca gtttttgagg gtaaaaagtg tttatgccat
     1021 aatgatttta gttgtaatca tctattgtta gatggcaata atagattaac tggaataatt
     1081 gattttggag attctggaat tatagatgaa tattgtgatt ttatatactt acttgaagat
     1141 agtgaagaag aaataggaac aaattttgga gaagatatat taagaatgta tggaaatata
     1201 gatattgaga aagcaaaaga atatcaagat atagttgaag aatattatcc tattgaaact
     1261 attgtttatg gaattaaaaa tattaaacag gaatttatcg aaaatggtag aaaagaaatt
     1321 tataaaagga cttataaaga ttgattatat aatatatgaa aagctattat aaaa
//
LOCUS       DAZUKZ010000236         1368 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000236 DAZUKZ010000000
VERSION     DAZUKZ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.245"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..286
                     /locus_tag="ACG8EF_002926"
     CDS             26..286
                     /locus_tag="ACG8EF_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948155.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(648..887)
                     /locus_tag="ACG8EF_002927"
     CDS             complement(648..887)
                     /locus_tag="ACG8EF_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948156.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1173..>1368
                     /locus_tag="ACG8EF_002928"
     CDS             1173..>1368
                     /locus_tag="ACG8EF_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948157.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTL"
BASE COUNT          478 a          188 c          250 g          452 t
ORIGIN      
        1 aaaaaacatt aaggatggat gaaatatgaa taacgaaact atcattttag gaaaaaaata
       61 tatttgccaa cctattggct tgaaacaaaa agtggttgga gaagtaatca gtaaattaga
      121 aaattgtgtt gtgatctgcg tagacgaata tcatgacgtc gatcgcaatg aaatattaga
      181 aaaatgcgga aaagtagtgg caaaatatac tcacgtttat gagttggaaa caacagaata
      241 tctatataac tatagcaaca aatatgaaca tactttagtt tcataaagat ttaaataaag
      301 tgccttcaaa agcgagaaga ccaagctttt gaaggcactt tatttagttg gcagtgattg
      361 ttcatgattt ttgatttatt caaaaggttc gcctagagaa tctgtctctg taacgaggcc
      421 tggtggagaa actcgataaa aacctatact gccagaactg tcctattttc ggatagacaa
      481 actagtagtt ttaaaaagat catccgatcc aatcatttca aaaaatgcaa aactgatatc
      541 aggatcattt agctcaggct tgttatcgta aagcatttgt aaaggttgca tttagttagg
      601 agtaaacgcg aaactatcat cattatttgc tgaagaagat gaagaagcta attcagagtt
      661 tgtttgaaat aaaggagacg tgctgacagt ttctttaccc tgttttttgc agaggtagaa
      721 acttttatat cggaagtttc taccggagaa tttttctcat ttgtagaaat tgttgaatcg
      781 atactattcg aaatttgcgt atctgaaggt atgttctttg atgcagaagt cccacaagca
      841 gtaagtaaca ataaatttga taaactgata aacagaattc ttttcattat gtacacactc
      901 cttatagtat cgtttagcat acacctatgc tcagtattag gcaaaatcaa tgcaaaacta
      961 tcaagtcttt tatagtatct attagtctta gtaatacata tatggagagg gtgatatccc
     1021 agttcaaacc agatacattg gttaccatct gtattaacga ttatgcctct aaacgctttt
     1081 tttatgaaaa aatttataag tgaaaacgta ggtatgatat tatctagagg taattttttt
     1141 gggtatttaa atagttgcta ggaggatagc ttttggattt ttacagtaat tttatcttaa
     1201 taatagcaat attattactt ctaaatattt ggttttttga taagagtaga aatgcaggga
     1261 taggttttag aaccaaacga agtacgtctt ctgaaaaaaa gtgggtatat tcacagacta
     1321 ttttttacgg aggggttatt tcaatcagtt tactttcttc aacacttt
//
LOCUS       DAZUKZ010000237         1364 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000237 DAZUKZ010000000
VERSION     DAZUKZ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.246"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACG8EF_002929"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACG8EF_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFS4948158.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          392 a          242 c          217 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcggatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DAZUKZ010000238         1360 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000238 DAZUKZ010000000
VERSION     DAZUKZ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.247"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACG8EF_002930"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACG8EF_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACG8EF_002931"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACG8EF_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          485 a          220 c          182 g          473 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa
//
LOCUS       DAZUKZ010000239         1359 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000239 DAZUKZ010000000
VERSION     DAZUKZ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.248"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..>1359
                     /locus_tag="ACG8EF_002932"
     CDS             105..>1359
                     /locus_tag="ACG8EF_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4948159.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTR"
BASE COUNT          442 a          276 c          256 g          385 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaa
//
LOCUS       DAZUKZ010000240         1355 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000240 DAZUKZ010000000
VERSION     DAZUKZ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.249"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACG8EF_002933"
     rRNA            <1..1355
                     /locus_tag="ACG8EF_002933"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAZUKZ010000241         1325 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000241 DAZUKZ010000000
VERSION     DAZUKZ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.251"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..656
                     /locus_tag="ACG8EF_002934"
     CDS             117..656
                     /locus_tag="ACG8EF_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948160.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          496 a          175 c          211 g          443 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgagaacctt gggttttcaa gtgctttttg
       61 gttcattaat cttaaccata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
      121 ggagccttgt aggaatagtt cttattattt ttactgtgac tgcagcaaca gaagcaaatt
      181 attcgcataa aattcagtcg tataaaggtc gtttagttat gaccaaaaaa gatgttacac
      241 gttatacatc ctcaagaact ggaggcctat atgactatgc accattagat gcattgaaat
      301 ataaagatca tttaaagaaa catgaagtaa aagttggtga tcggtgggag aatgcatctc
      361 ataaagcata taattccaaa atagtctata cagtagaaga tgccgatgag caaaatatca
      421 cattacctgt attcaattat tggggaacag ttgtgaaagt aaatgggaaa aagagcacta
      481 tagaaaatga caatggttta gtgtctatta aaggtacggg gaaaagaact atagttgagg
      541 tttcatctac gtatccaact acgatgattc tatctttatt aattagttca ataactttta
      601 tattcttatt tacacgttat ttaataagat gctgcggtga aatgaaaata gattagatag
      661 atattataaa tatgttgatt ctattttcat agcaatggag aaaatacatt agcaatatat
      721 aaaagaaggt atcaagagtc acagtgaatt tattccgaaa agtgaaaaga gcaaatataa
      781 aatttaatga aaatatagaa ctaaatagga aaacgtagac gtaattttct acgttttcct
      841 tttcatatat attaaatatt acggaaaatt atataaacta atttagtgtt ttgtttgtaa
      901 ttcctaattt tgctccactt ttagataatt tttaggtcta tttaactcat cagacattct
      961 atgctcattt ttctccaaat ttgtttcaac tctttcgtac ttaattttgc gtatttgaaa
     1021 attcttcttt tagagtactt ttattacgat actagtatga tatatatcta tagcaaacag
     1081 ttaaaatctt cctaaaataa gcgaatagca ttcctaaaac ctataaaatt tttagatgaa
     1141 gcgacaagtg gacaattaat aaactacttg aacatgaaat tatggatcac ctttcaaatt
     1201 tagaaaatgg aattcttata cttattgcgc aacatttatt gattgcaaaa ttatgtgaca
     1261 aaaaaaacta gtagaagaaa gaataatggc ttgataataa agtactacct attaaaggta
     1321 gtact
//
LOCUS       DAZUKZ010000242         1324 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000242 DAZUKZ010000000
VERSION     DAZUKZ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.252"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACG8EF_002935"
     CDS             278..820
                     /locus_tag="ACG8EF_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948161.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..>1324
                     /locus_tag="ACG8EF_002936"
     CDS             976..>1324
                     /locus_tag="ACG8EF_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFS4948162.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DAZUKZ010000243         1310 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000243 DAZUKZ010000000
VERSION     DAZUKZ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.253"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..>1310
                     /locus_tag="ACG8EF_002937"
     CDS             40..>1310
                     /locus_tag="ACG8EF_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFS4948163.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFL"
BASE COUNT          443 a          256 c          252 g          359 t
ORIGIN      
        1 atcaccacaa aataaacaac tgagaggacg atacgctata tgaatgattc tatcaaaaaa
       61 atgctgagaa taacagagaa agatttgatg attacagagg tctcttacga gacccttcag
      121 aagaaaaaga cgttggtcgt cgatgctgtt ctctcgccta ctcctcgtgc ttgtagaagt
      181 tgtggttcta ctgtggtaga tggaaacggg aaagcaatta tagtgaaaaa tggaaaaaag
      241 gaaacgattg tccgttttga acaatacaat catatgcctt tggttatgcg cctaaaaaag
      301 caacgctata cctgtaaaaa ctgtcgcacc cattggacgg ctcaaagtta ttttgtccaa
      361 cccagacatt caatcgcaaa tcatgttaga tataaaattg cttctttact gactgaaaaa
      421 gtatctttat cttttattgc gaaaagctgt caggtatctt tgaccaccgt tattcgtaca
      481 ttgaaagagt ttaaaagcta tttaccaaag caatctaaga agatcctccc cagagtattg
      541 atggttgacg aatttcgttc gcatgcttcc atagaagata agatgagctt tatttgcgcg
      601 gatggcgaaa caggaaaatt aatagatgtt ttgcctacgc gtaaattacc tcgattaaca
      661 agctatttct taggttgtac caatccagaa gaagtagaat tcttggtgac agacatgaac
      721 gccgcctact tccagctcac caaacgtgtt ctgccaaatg cgaaagtcgt gattgatcgg
      781 tttcatattg tcaaacacat gaatcaagcg ttcaatgagt tgcgtatccg tgaaatgaat
      841 gaacttcgta aagccggaca gaaaagccag gcagaaaaac tgaaaaagaa ctggcgcttt
      901 ttgctaaaaa atcgtgcaaa catcaaccat tatgaataca aaacatggaa aagtttccga
      961 gcaccaaaat acccatttct tactgaagca atgatgattg atcgattgct tgagttttct
     1021 ccgcccctaa aggaggcgta tccctttttt catgaattag ttgaagcctt tcgagacaaa
     1081 gaccctgact tatttttctc cttattggca gaacttcccg aaacgttaga tgacagcttt
     1141 cgggaaaagc ttcaaaacct tctgacctat gaagaaggca tcaccaacgc aatgatctat
     1201 ccttattcca atggaaaaat agaagcgaag aatacccaca taaagacaat gaaacgagta
     1261 tcctacggat ttaaatcatt tgagaacatg agaattagaa tctttttgat
//
LOCUS       DAZUKZ010000244         1287 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000244 DAZUKZ010000000
VERSION     DAZUKZ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.254"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..848
                     /locus_tag="ACG8EF_002938"
     CDS             <1..848
                     /locus_tag="ACG8EF_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4948164.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          418 a          235 c          252 g          382 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttt
//
LOCUS       DAZUKZ010000245         1270 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000245 DAZUKZ010000000
VERSION     DAZUKZ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.255"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..609)
                     /locus_tag="ACG8EF_002939"
     CDS             complement(<1..609)
                     /locus_tag="ACG8EF_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS4948165.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSN"
     gene            complement(609..>1270)
                     /locus_tag="ACG8EF_002940"
     CDS             complement(609..>1270)
                     /locus_tag="ACG8EF_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4948166.1"
                     /translation="KKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPD
                     WHPWEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDS
                     FTLIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTD
                     IQLYDEVMHDNNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEV
                     N"
BASE COUNT          433 a          215 c          142 g          480 t
ORIGIN      
        1 attactgtta atttcaagat tcgaatattt accaaactgt ttttttactg atttagggaa
       61 accaaatagg tttataatat aatccaattc atgtatacag tctaattcta ctcctccccc
      121 catagattta aaggcagaat aattatttct ataatcagta ccttttctcc aatcaggaag
      181 ataagtagaa caaatagctc ttacagaata tactttttta ctttgaatac tattacgaac
      241 aacttccatg atatttttat acctcagtgg agcagcaaca taaatatttt tgtaatcata
      301 attatattga gtaagatcaa tactctcaga aaaagcaggt ttttctaaaa atatgtaatc
      361 aactttttca acaattacat ttagtgtttc cagatgctta aaagtcggat ttgtaataaa
      421 agctatatca taatgtgtat ccaaatcgtc tctattttga atatacgtta tttcttcaaa
      481 ctcttttttt ccactgctac tataagctgt tatatcaaaa ttaatagaat tcttttttcc
      541 atagcttatt agatttttta gatgtctctt tccaattgac cctaacccaa aaaaaattac
      601 tttcatcatt aatttacctc aatgtcgtta attaagttaa taatatactt atctttttca
      661 aaagtatatt ggggttccat tttcttttcc aaaagcgtga aaaattcttt aatctcctct
      721 aaatatgcat cttcaataat agtatgtgaa taattattat catgcataac ctcatcgtac
      781 aattgaatat ctgtcatact agaagtttct tcattccatg aaaatagaga atccgggtta
      841 ccttcccatt ctattaaaat cttttcacca attatagtta aatttctttt agctactctt
      901 gaaacaacat ctacacttaa agttccaact ataccagttt catgctcaat aataagagtg
      961 aaactgtccg aataatcaat atcaagattg gataatttct gggtcattac agtaaacgat
     1021 ttaactttcc caaatgtatc tataatccaa ggtaattcaa tagcgaatat ttctctacac
     1081 ccattagttt ttttgtttga aacaaaaaaa ttgcgataat cttcccaagg atgccagtcg
     1141 ggtaaatact gtcctacatg gtattgataa gaaatttttt tattatgcgt cactttagac
     1201 ttaatatatt gaatttcttt tctcttcaaa aatgtagaag atagataaag taatctatct
     1261 ttctttttag
//
LOCUS       DAZUKZ010000246         1236 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000246 DAZUKZ010000000
VERSION     DAZUKZ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.256"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..539
                     /locus_tag="ACG8EF_002941"
     CDS             66..539
                     /locus_tag="ACG8EF_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFS4948167.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            548..775
                     /locus_tag="ACG8EF_002942"
     CDS             548..775
                     /locus_tag="ACG8EF_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4948168.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          392 a          197 c          206 g          441 t
ORIGIN      
        1 aaatagttgt tttacaataa ttattctgct atattttagc tatcaaagat aaaagaggga
       61 taaaaatgaa atttaaaaca ttgctattaa aatttgtcgt tttaggtata agtgccgtat
      121 ttattttgtt cggcgtactt ttttatactc agatcgctac aagcgaaaaa ggattcaatt
      181 ttgattggca gaccacatta tttgtttctg tcatttactt tgcccttctc ctagggatcg
      241 ttattgctta tttcttattg aaactattga aaaaaatcga agataaccaa gctttttcca
      301 ctcaaagctt aaacatacta aaaaaaatcg aacgaaccat tttgtttatt ggtttcgcct
      361 tttttggctt acttcctaaa tttatgaaag tagcggattt agacgatgct ccaggtttga
      421 tgttggtagg aatcgctgtc gtattcattc cttttgcagt atacacatta acaagcgtgc
      481 tggaaaaact gcttgaacat gcgattttac ttaagacaga tcatgatttg acagtgtgag
      541 gtgcgacatg gcaattatcg ttaatttaga tgtagtatta gctcaaaaaa aaatgagtgt
      601 gactgaattg agtgagaaag tgggcattac gatggcaaac atctctattc taaaaaatgg
      661 aaaagcaaaa gccatccgtt tttccactct cgaaaaaatc tgccaagttt tggagtgtca
      721 accaggtgat ttgttgacct ataaaaatga ctcgaatact ataccagaag aataaaataa
      781 agtagaacaa acagtaaata aaaaataatc tgaatgatag atgtgtgaga cttttagaag
      841 ttatttacgt ctattattca gattttttat ttcgattgaa taccgtttta tctataaata
      901 agatatgtac tgactcatac aaagggggcc ttttcttctc catgtactat cacttttcct
      961 gatacaactg gtttcctgtt acgttgtagt tggatctgct tttagtataa tactttattc
     1021 attccaccag actgatagca gtttgagcag tctaaataat catgtagtat tacaggccat
     1081 tctgtttcat accttgaagt gcttaaaact tcagctaatg gcccattgtg ttaactctta
     1141 ctccattctt ttttagtaag ctcccatata tactgtaaag ccttctatat attatgtcat
     1201 gttgcttttt aacatttact atgtagtttt gataaa
//
LOCUS       DAZUKZ010000247         1221 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000247 DAZUKZ010000000
VERSION     DAZUKZ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.257"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1091)
                     /locus_tag="ACG8EF_002943"
     CDS             complement(<1..1091)
                     /locus_tag="ACG8EF_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948169.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGFFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTG"
BASE COUNT          481 a          213 c          125 g          402 t
ORIGIN      
        1 cctgttttca atccaaattt tttagttagt cttatcggtt tcacaaaaaa taaccaaaac
       61 atccatataa taaaaactat acatccaact tctatataaa tagttaatat atcactgtgt
      121 aggcttgctg caattcttcc accatttaac ctaaagtgcg ataaataaag attaaaaaag
      181 tattttgaaa accatgtcca tcctgttcct acatatccta aaccaagttc ataaaattta
      241 gatgaatata gataaaatga aagcctcgca ccgtcatctg taatgcccaa atttgacata
      301 agcaatgcca aaattcctgt atctataatg taaataaaca ctaacgaaat aacagttgca
      361 caaatagtac acactaatgc acggatttga atcttttttc cccactttaa taaaacataa
      421 tacactagaa ttgcaccact taaagctaac agttcaattc tcttcaatcc catgaaaatt
      481 aaaattaaag atacgataat ttttatctta tgtttttttg tatttttatt ttcaaaaaat
      541 acgtaataaa ggaaaaaaaa ccccatcgca aaagttaaat catgtacctc catagcaaaa
      601 tttgcagtat ttgtttcacc tgttataaga ctttttatat aagaaattaa accatttaaa
      661 ccataagata caattaaata aaaaactgaa ccactcatat atgaaataac acctgaccaa
      721 aaaataaaat caattccttc tgccttaaat aagtaaaatg ctgtacatgc gtaaccatat
      781 gcaagaatta aataaagcaa cgtggaacat aactttataa aattttcaaa ttctgcattt
      841 gaaccaattc cccatattat tagagtatag atagaaaata tcacaaacgg cattatagtc
      901 aaccaaaagt tattcttgat aacgtatttt ttatatgttt tctcacgata agaaaaatag
      961 cttaacataa aatataaatc ccatattgcc ataataaaat atcgcataga taaactagta
     1021 ttaattcccc aaaatcggga tgttaataca attattgtac ttaccataaa tagcattttt
     1081 tttacactca tcgtttcgcc ccgtctacat atatttgttc tatatacgag tttaataaac
     1141 tgatattttt tgcgctttaa gtgaagaaca ttaaataatg gtattgtact gatttttcca
     1201 tacaagtttt ctttaatgta a
//
LOCUS       DAZUKZ010000248         1212 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000248 DAZUKZ010000000
VERSION     DAZUKZ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.258"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..383
                     /locus_tag="ACG8EF_002944"
     CDS             <1..383
                     /locus_tag="ACG8EF_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4948170.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
     gene            complement(367..960)
                     /locus_tag="ACG8EF_002945"
                     /pseudo
     CDS             complement(367..960)
                     /locus_tag="ACG8EF_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
BASE COUNT          369 a          241 c          212 g          390 t
ORIGIN      
        1 catttagccg tatcaaacaa cctctgacag aggttgagat ttttcatact gatcgtggaa
       61 aagagtttga taaccaagct attgatgaat tattaacaac ttttgacatc aatcgatcat
      121 tgagtcataa aggctgtcct tttgataatg ccgtagctga atcaacttat aagtcgttga
      181 aagtagaatt tgtctatcaa tacacatttg aaaccttaca acaattggat ttggagttat
      241 ttgactatgt aaattggtgg aaccaccttc ggttgcacgg tacacttggc tacgagacac
      301 cggttggtta ccgtaaccag agattggcgc agcgaatcct tgataatgag ctcggatgtg
      361 ctaacgctag cgaggcagtc taactttaac tgttagctcc tgccgaagat cgtcacatcc
      421 gaggaggctc attgtcaagg acaatcggaa tagcatacgg aaagaagttc tgcaccttat
      481 aaaatttgtc aaaaaaactg ttgccattcc accagacact tgcttaaagc gaaaaatttc
      541 ttcaatgtcc atgcctaatt ctttggatat tctcttgtct gaccatccat tttttactaa
      601 tctttggatg acaatttcca ttcctcttat ttcatgggtc cctcttgcac gattgtgtcg
      661 aattgttgaa gccatacgtt cttatatggg tttatcaata actgttaccg gtacaacttt
      721 taagttgagt ctttccttag caacacgata cctatgaagc acatcaacca agatatactt
      781 ctcttctttt tccttgtaat aacaaactaa tggttgagta aagccatctt caatgataga
      841 aagctccaat aagttcattt ccggagacgc tactcgattg ggattataat cgttcgcctg
      901 tattttggta attggaacaa gccgaacgtc catcacaggg aaagttatgt ctttattcat
      961 acgcttgtat accctacttt cttaaaaagt agggtatact caagaatccg attaattatc
     1021 ttctcaattt ggataatata tctcatcagt cttcttgtca ctttatcaac atgattttgc
     1081 tgcaaaatgg gataccgaca gtactggact ggtaatcagg ataaaagcaa aaataattaa
     1141 tttgtatcct aaaaaatcca taaaaattct ctcacatttt cgggtattaa ttcgagaatc
     1201 acacctataa at
//
LOCUS       DAZUKZ010000249         1205 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000249 DAZUKZ010000000
VERSION     DAZUKZ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.259"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1205
                     /locus_tag="ACG8EF_002946"
     CDS             <1..>1205
                     /locus_tag="ACG8EF_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFS4948171.1"
                     /translation="QKKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVV
                     IDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFKSFENMR
                     IRIFLINQL"
BASE COUNT          399 a          234 c          232 g          340 t
ORIGIN      
        1 ttcagaagaa aaagacgttg atcgtcgatg ctgttttctc acctgctccc catacctgta
       61 gaaattgtgg ttctactgtg gtagatggaa acgggaaagt aattgtagtg aaaaatggga
      121 agaaggaaac gattgtccgc tttgaacaat acaatcacat gcctttggtt atgcgcttaa
      181 aaaagcagcg ctatacctgt aaaaactgtc gaacccattg gacggctcaa agttattttg
      241 ttcaacccag acattcaatc gcgaatcatg tcagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa attgtcaggt atctttgacc accgttattc
      361 gtacgttgaa agagtttaaa agctacttac caaagcaatc taagaggatt ctccccagag
      421 tattgatggt tgatgaattt cgttcgcacg cttccataga agataaaatg agctttattt
      481 gcgcagatgg cgaaacagga caattaattg atgtcttgcc tacgcgtaaa ttgcctcgat
      541 taacaagcta tttcttaggc tgcgctaatc ctgaagaagt aaaattttta gtcacagaca
      601 tgaacgccgc ctacttccag cttaccaaac gtattctgcc aaatgcgaaa gtagtgattg
      661 atcggtttca tattatcaaa cacatgaatc aagcctttaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagta ggacagaaaa gtcaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 ttcgagcacc aaaataccca tttcttacgg aagcgatgat gattgatcga ttgcttggct
      901 tttctacgtc cctgaaggag gcgtatccct attttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgacg
     1021 gctttcggga aaagctccaa aaccttctga cctatgaaga aggcatcacc aacgcgatga
     1081 tctatcctta caccaatgga aaaatagaag cgaagaatac ccatataaag acaatgaaac
     1141 gagtatccta cggatttaaa tcatttgaga acatgagaat tagaatcttt ttgatcaatc
     1201 aatta
//
LOCUS       DAZUKZ010000250         1193 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000250 DAZUKZ010000000
VERSION     DAZUKZ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.260"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACG8EF_002947"
     CDS             <1..1076
                     /locus_tag="ACG8EF_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS4948172.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAZUKZ010000251         1183 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000251 DAZUKZ010000000
VERSION     DAZUKZ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.261"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..153
                     /locus_tag="ACG8EF_002948"
     CDS             <1..153
                     /locus_tag="ACG8EF_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS4948173.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            207..575
                     /locus_tag="ACG8EF_002949"
                     /pseudo
     CDS             207..575
                     /locus_tag="ACG8EF_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            681..944
                     /locus_tag="ACG8EF_002950"
     CDS             681..944
                     /locus_tag="ACG8EF_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4948174.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1183
                     /locus_tag="ACG8EF_002951"
     CDS             944..>1183
                     /locus_tag="ACG8EF_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS4948175.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVLLRSLKIKR"
BASE COUNT          448 a          154 c          239 g          342 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacac atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggttctgt tgcgaagttt gaaaatcaaa cgc
//
LOCUS       DAZUKZ010000252         1142 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000252 DAZUKZ010000000
VERSION     DAZUKZ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.262"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(98..1024)
                     /gene="cysK"
                     /locus_tag="ACG8EF_002952"
     CDS             complement(98..1024)
                     /gene="cysK"
                     /locus_tag="ACG8EF_002952"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFS4948176.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT          304 a          270 c          177 g          391 t
ORIGIN      
        1 ctctattcaa ataatttttc tcatcataaa tttttgtgtg ttcttttcat ctttaaaagt
       61 tttttacata aacctatctg gagaaaaacc aagtgcctta tatctcttgg taaagggctg
      121 tagataaata acgttcacca ttgtcgggaa ccacagctaa taccttcttg ccttttccta
      181 attcttttgc gacttttagc gcggcagcaa ttgcagcgcc agatgagatg cctactaaaa
      241 tcccttcttt tcgcccaact tctctagctg tttccatcgc ttcgtctcca ctaatagaaa
      301 ggactttatc atacacagaa gtatccagtg ttttaggaac aaaacctgta ccgattcctt
      361 ggattttgtg aggtcccgct tctttccctt ctaaaatagc agattctgct ggttctaccc
      421 cgattaattc gattttcgga tacacacgtt ttaattctcg gccagcgcca gtgatcgttc
      481 ctcctgtacc gatacctgca acaaacgcgt ccaagccatt tactccaaat gcttgatgta
      541 tctcaggacc agtcgtcttc tcatgcacaa gaggatttgc ttcattttca aattgtaatg
      601 gtaaaaagta gccgttttct tttgccaaac gttctgcttc tctgattgat ccagaaatac
      661 catccgcacc aggagtcaaa atcaactctg cgccgtaccc tttcatcaat aaacgtcttt
      721 cgatactcat cgtctcaggc atcacgatga ttactttata tccttttgca actcctacca
      781 tcgacaatcc gattcctgta tttccagaag taggttcaat gatagtatca cctggtttca
      841 gtagaccatc gtgttctgct ttttctatca tacttaaagc aatacgatct tttacacttc
      901 ctcctggatt aaaaaactca agctttacaa aaacatctgc tgaatcttct ggtacgattt
      961 tatttaattt gacgattggc gttttgccaa tcaattcggt gactgagtta tagatagctg
     1021 tcatcttcaa ttcctccttg tctttgctct tcttacttat cataatacaa tctgttcatt
     1081 tatggcgagt gattcgtctt tgactagcaa aaaaaagaca aagatatacc atatatggta
     1141 at
//
LOCUS       DAZUKZ010000253         1142 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000253 DAZUKZ010000000
VERSION     DAZUKZ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.263"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..922)
                     /gene="xerC"
                     /locus_tag="ACG8EF_002953"
     CDS             complement(17..922)
                     /gene="xerC"
                     /locus_tag="ACG8EF_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFS4948177.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          378 t
ORIGIN      
        1 gttcttttcc ttatttttac gctcttggat ggaaagaacg atagtttttt tgtaaacttt
       61 cttttgttac atgggcatag atctgggtag tcgataaatt ggcatgtcct agcaattctt
      121 ggactgtccg caaatctgcc ccattattta ataaatgagt agcaaatgta tggcgcagca
      181 tgtgaggatg gatatcacta tccaaactgc tttttttgat catttgattc aagacatatt
      241 caattccagt aggcgtgatt ggttttccat gatgattcac aaaaacgata tcatgctctt
      301 cgtgatattt tgtcatcaat acctggcggc cttccctgaa atattcttgc agagcatctt
      361 gagcaaaaga acctagagga gcatatctct ctttgttccc tttaccatgg atcaacatga
      421 cctctgaatc aaaatcaatg tctgataccg acaaactgct acactcactt aaacggatgc
      481 ctgacccgta aaggacttcc agtaaagcgc ggttcctcaa atccaaagga ctatctcctg
      541 ctacgctgtc aaaaagtgct tgcatttcat tttcataaaa aaaacgtggc agtctcaaat
      601 ttttcttttt gagatgaacg taagaaaatg gattttcttt tatcacttct tctttgagaa
      661 gatactgata aaacgaacgt aggctggcta tcttgcgact gatagaattt ctgctatagc
      721 taggacgatg atcagctaat gcactcaaat agacacgaac cgtaaaatat tccgccttca
      781 gcagatcagc gtctccagat tcctccaaaa aatgtttgaa atggagaatg tcttcttcat
      841 atgcttcttt cgttttttct gaatatcctc tttcgacaat caaataacgc aaaaattttt
      901 gttcccacgt ggcttctgtc attttctcac cttccttttg tcccagagaa taatttagca
      961 cagcgtttat aaaaagacaa ataaattgtc agaatttaac caatatttct aagtttgttc
     1021 acaattggcg caattcctca cattttgttt gagaaattct taagaaaaac gcaagaaaaa
     1081 gggctctata atttatggga ctgatgaatc agaattgatt cttcagcccc attttcattt
     1141 ta
//
LOCUS       DAZUKZ010000254         1133 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000254 DAZUKZ010000000
VERSION     DAZUKZ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.264"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..422
                     /locus_tag="ACG8EF_002954"
     CDS             12..422
                     /locus_tag="ACG8EF_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4948178.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            436..792
                     /locus_tag="ACG8EF_002955"
     CDS             436..792
                     /locus_tag="ACG8EF_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4948179.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          395 a          170 c          217 g          351 t
ORIGIN      
        1 tgagtgagaa aatgataaaa gtatatactt cgcctagctg tacttcctgt agaaaagcaa
       61 gagcatggtt gcaagcgaat caacttgaat ttgaagagaa gaatattttt gccgaaccgt
      121 taacagaaag tgagatcaaa aagatcttgg cagcgactga aggaggagtt caagatatta
      181 tttccactcg gtcgaaaatt tacgaaaagc tgggcataga ttttaacgaa ttaactttaa
      241 agcaaatgat cgtcttattc aaagaatatc cttcattatt gagacgacct attttgattg
      301 atgaaaaaag aatactaatc ggatttaatg aagatgagat ccggtcattt gtccctcgtg
      361 aaattcgatg tgttgccaga aatctgatct atttacaaaa caataacatc ttaaattcat
      421 agggagggag ccttcgtgga agtgaaagtc tatggatcag cgcactgtgt aacaagtctg
      481 aaggcgaaac aatggttaga acaccatcaa ttgccttatc aatttgttga cttagaagaa
      541 cgcgatctaa gtacagaaga agcagaagaa ttatgcagtt ttgaagaagt gcaagcagaa
      601 cagctatttg cgacttggtc tgaggctttt cacaacattg cgattgacct ttcctgtgta
      661 ggaaaaaaac aactgatttt tttatgcaca caacatacca atctcctcag acgtcctttg
      721 attattatcg atgaagttct atttattgga tacaatgagc agttgatgga acagcttatc
      781 ttaaataaat agactttaac tgttttaatc aagtcactga gcatttcata cagttgtaac
      841 agtcgaacac ttagcatcaa gcgtgttatg tgttcttttt ttgactttat ttttctacta
      901 tagagacatg ctcaatttat gtggtcaaaa ttcaaatcta ggtggggata tagagttaaa
      961 aatatattga catgtgtaaa tttacatgat atattcattt ctgttgtttt aagtagcaaa
     1021 gcaataaaat agtattttaa aaaacatata aaatagttgt tgacatgtta gaaatgacat
     1081 gataagataa ctgagttgtt aaaaaagaac cacttgcgaa gcttgattct atc
//
LOCUS       DAZUKZ010000255         1106 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000255 DAZUKZ010000000
VERSION     DAZUKZ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.265"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACG8EF_002956"
     CDS             <1..1087
                     /locus_tag="ACG8EF_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4948180.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          403 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaa
//
LOCUS       DAZUKZ010000256         1064 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000256 DAZUKZ010000000
VERSION     DAZUKZ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.266"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..>1064)
                     /locus_tag="ACG8EF_002957"
     CDS             complement(39..>1064)
                     /locus_tag="ACG8EF_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFS4948181.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
BASE COUNT          346 a          177 c          160 g          381 t
ORIGIN      
        1 ttttagaaaa agcagtcgga ctttctaaaa gaagttcact attcgatcct gtcgatgatt
       61 tcttcaggaa aatcgtctat ttgcttgtca attttgctgt ctttgtattt ctcttcttgg
      121 atagtcaaca aaatctttgt aagattcccc cagtctactg ctcttggcgg atcgagccaa
      181 accactactt gagatgcact ttcttcatat agattattcc tattcgtgat tataacatcc
      241 gggaactcag gctttcctgc ctctatcact tccgtgttca gattaatgta caagcttaaa
      301 tttttcttga taaattgatt ataggcagca ccataagaaa aatcgatgat gatctttact
      361 ggttcagaaa tcagttgaat gggtatgtga ttgatcaata ataataaata actgaaaaaa
      421 agaaaaaatt tatcttgaag caactctttg cgttttgacg ttagatcatt tatatactct
      481 atcaaatagc aataaaatgc tggatagttt tctctaaaaa aagaaacatc aagtggaaat
      541 tcatcagctt tattaatagt gtaatgcaaa agtttaaaat gtattttggt taactctctt
      601 cggatcaatt ggctcgtctc ttctcgcaac agattaaact gattttcaaa actttcaatg
      661 aatcgttcat tcaaccattc gattgtttca tcttcatttt ttctcaagta tttttgatcc
      721 aatttatcat gaagataaag ccaagagagt gtcttattga taaaaataac ggatttttct
      781 cctaatactc cttttaagtg gtaaaatcct atatccgaaa aaaatagcga ctgattgcta
      841 gcaatatcta aagaatcagc ttgttcagcc aacaggttga ttgctaaaaa gtggaataat
      901 ttcttttttt cataagcaga cagaggataa tactgtttta aatcttttaa cattttttct
      961 gtttttataa ttataggtag cgtatacaat tccacaaaac ttatctctgt tgagttaaaa
     1021 agattatata aaaaaagccg aatgttttta ggattccctg tcaa
//
LOCUS       DAZUKZ010000257         1051 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000257 DAZUKZ010000000
VERSION     DAZUKZ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.267"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACG8EF_002958"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACG8EF_002958"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFS4948182.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DAZUKZ010000258         1039 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000258 DAZUKZ010000000
VERSION     DAZUKZ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.268"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            406..1039
                     /locus_tag="ACG8EF_002959"
                     /pseudo
     CDS             406..1039
                     /locus_tag="ACG8EF_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          358 a          183 c          186 g          312 t
ORIGIN      
        1 ttattaataa aatatttttt atattacaaa agtagtgttt caaagttagt agagaaaatg
       61 tagttatata tttaaatttt taccagaaag aaatgaacac cttcaattat ctaaatctcg
      121 aaaatggtta gatgtaatca tcaggaaaaa caatcttaaa cgaattacta ttcatggtct
      181 tagacacaca catgctagct tgcttttagc aagctagtgc aaatattaaa gatgtgcaag
      241 agcgtttagg ccactcttct attcaaatca ttatggatct atatattcat attacagaca
      301 aaagaaaaga agaaaccgta gcgcaattcg caaaatatag gctctataat ttatgggact
      361 gatgaatcaa aattgattct tcagtcccat tgttatttta ggtatatgat tcgtacattg
      421 aaagagttga aaaactatct tccgaagccg tcaaaaagga ccctgccaaa agtattgatg
      481 gtggatgaat ttcgttcgca cgcttcaatt gtggataaaa tgagctttat ttgtgcggat
      541 ggcgagactg gaaaactcat tgatattttg cctacacgaa aattgtctcg attaacacac
      601 tattttagtc gctgtgcaaa tcctgaagcg gtggaatatt tagtgacaga tatgaacgcc
      661 gcctacttcc aactgacaaa acgtgtctta ccaaatgcga aagtcgtcat tgattgtttt
      721 catatcatca aacatttgaa tcaggcgttc aatgattttc gtgtacaaga aatgaaaaga
      781 ttacaccagt tagggcaacg acgggaagcc gagaaagtga aaaagaactg gcgctttctt
      841 ctaaaaaacc gtcaaaaaat caatcactat gattacaaaa catggcgcag ttttcgtgca
      901 cctaaatacc cgctactcac tgaagcaatg atgattgatc gattgctggc attttcaacg
      961 cctttaaagg acgcctatac ttttttccat gagctgaccg aagcctttag agacaaggaa
     1021 tcagacttat tttttacct
//
LOCUS       DAZUKZ010000259         1024 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000259 DAZUKZ010000000
VERSION     DAZUKZ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.269"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1024
                     /locus_tag="ACG8EF_002960"
     CDS             <1..>1024
                     /locus_tag="ACG8EF_002960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4948183.1"
                     /translation="RMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQEYDKV
                     ILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEIA
                     EAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKI
                     EKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIG
                     DCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFNYNFYA
                     AGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQLCSQED
                     LSGLLSM"
BASE COUNT          399 a          121 c          180 g          324 t
ORIGIN      
        1 agaatgacac taaattctga gataaaaaag ttatttctag agacagaagt tgacaaaatt
       61 gatattcaaa ataaaaagat tagttgtgta aaaaaagatg gtacaagatt ctctcaagaa
      121 tatgacaaag ttatattagc tacaggttca aagcaaatta tgctagatat accggggaat
      181 aatttagcta atattttttc gataaagaat tttccaaatg caaaagagat ttttaaagct
      241 ttggatgata gtactattaa aaaagtagga attattggag caggatatat tggagttgaa
      301 attgctgaag ccataagaaa aagagggaaa gaagtatatc ttttcgatgt tgcagataga
      361 gtgttgtcga cttattacga ccgtagtttt tcagataaag ttgaagaaat actatcaaaa
      421 aatggaatac atctttgtct atcagaaagt cctaaaaaat atttaggaag aaaaaagatt
      481 gaaaaaatag tcactacaag tggtacccaa tactctatgg atatggtcgt acaagctata
      541 ggatttctat ctaatagtcc aattggggaa aaggaactct tgagagatgt tagtggagca
      601 tatctaacta atgaatacca gcaaacaaat ataaaagata tatatgctat tggggattgt
      661 gctacaactt tttttacgtc cattaataga agtagtgttg atttttctat ttctaatgct
      721 ctaagaacag cttatattgc aactcaacat attaacaatc aagattttac accttctggt
      781 tcccaattta caaatggctt ttctatattt aattacaatt tttacgcagc aggtttgaat
      841 ttaaaaacag cacgattatt aagtgagtct atagattata tagaaataga agatttcatt
      901 agacctgctt ttttgagaaa aaatagtcgt gtaaaattaa gaattagctt tgaaaaaaat
      961 acaaagagaa tagtaggtgt acaactttgt agtcaagaag atttatctgg actattatca
     1021 atgt
//
LOCUS       DAZUKZ010000260          995 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000260 DAZUKZ010000000
VERSION     DAZUKZ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.270"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..913)
                     /locus_tag="ACG8EF_002961"
     CDS             complement(26..913)
                     /locus_tag="ACG8EF_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4948184.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          321 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaa
//
LOCUS       DAZUKZ010000261          994 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000261 DAZUKZ010000000
VERSION     DAZUKZ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.271"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>994)
                     /locus_tag="ACG8EF_002962"
     rRNA            complement(<1..>994)
                     /locus_tag="ACG8EF_002962"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAZUKZ010000262          986 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000262 DAZUKZ010000000
VERSION     DAZUKZ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.272"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..704
                     /locus_tag="ACG8EF_002963"
     CDS             <1..704
                     /locus_tag="ACG8EF_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4948185.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          333 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataat
//
LOCUS       DAZUKZ010000263          959 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000263 DAZUKZ010000000
VERSION     DAZUKZ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.273"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..511)
                     /locus_tag="ACG8EF_002964"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="ACG8EF_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="ACG8EF_002965"
     CDS             complement(570..>959)
                     /locus_tag="ACG8EF_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4948186.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DAZUKZ010000264          943 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000264 DAZUKZ010000000
VERSION     DAZUKZ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.274"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACG8EF_002966"
     rRNA            <1..>943
                     /locus_tag="ACG8EF_002966"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAZUKZ010000265          931 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000265 DAZUKZ010000000
VERSION     DAZUKZ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.275"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>931
                     /locus_tag="ACG8EF_002967"
     CDS             <1..>931
                     /locus_tag="ACG8EF_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4948187.1"
                     /translation="HCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFY
                     RPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFS
                     RFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETT
                     VRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          286 a          190 c          180 g          275 t
ORIGIN      
        1 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
       61 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
      121 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
      181 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      241 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      301 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      361 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      421 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      481 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      541 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      601 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      661 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      721 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      781 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      841 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      901 gtacggcttt cgcaactttt acaatttcaa a
//
LOCUS       DAZUKZ010000266          930 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000266 DAZUKZ010000000
VERSION     DAZUKZ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.276"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..441)
                     /locus_tag="ACG8EF_002968"
     CDS             complement(<1..441)
                     /locus_tag="ACG8EF_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFS4948188.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIH"
BASE COUNT          298 a          163 c          158 g          311 t
ORIGIN      
        1 atggattgag cgctgatact gttgttttcc ccgtttttcc tgatggccat tgggttttct
       61 tttacctttc atcggtagtg tgtcaatatc aaatattcct ttagaaaata aacgataaag
      121 tgttctcata ccgcatgaaa caggcctttc ttttcgcccg ataatgacgt caggcgtcca
      181 accaagagtg actttctctt taatgtaatc tacttcatga gcaggtaatt gaatgacttt
      241 tctaccacat tttttcttat tttctttata ctggtgccaa taatcaagag cagtcttgcc
      301 ttgcttgaac ttattgacta cattataaat agtttgtata gcacgtccca ttcggttagc
      361 gatttcaacc ggcttattat gttgaagata atatgattct atcattgtca gttcgtctat
      421 ggtaagatgt ttgtaggtca tttatggtta cactcctttg ttttctttcg tcggaaatac
      481 aatttgagtg taccataaat gcctttttat ttttctaact taattttaca attcgcgata
      541 tattaaacaa cgttgtcgcg cttcattgaa acattttatg aaaattccaa aaaagagttg
      601 acacatgttt ttagacgtga taagataact aagtcgttaa aaagaaccac ttgcgaagct
      661 tgattctatc gaaaaaaaac ttcaaaaaag ttcttgacaa ataacaaacg ataagttatg
      721 ataaatgagt tgctgaaaga tttcgaaaaa cgaaatcgaa aaaaactttt aaaaaagagt
      781 tgacaatcgc ttgtctctct gatatgatat aaaagttgct acggcagcca aaaacgaatc
      841 gggaaacact cccgatgaag tagacctttg aaaactgaac aaagtaagac aaaccaaatg
      901 tgtagggcgt cttgattcaa ttcaagacaa
//
LOCUS       DAZUKZ010000267          913 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000267 DAZUKZ010000000
VERSION     DAZUKZ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.277"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>913
                     /locus_tag="ACG8EF_002969"
     CDS             <1..>913
                     /locus_tag="ACG8EF_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4948189.1"
                     /translation="FLIQFAKGLEKVKAMGIAGIISTIVTIIANIFFLLILKWGLIGF
                     FIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPLIATVVGWWINSTA
                     DRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKEYGDNETAKFYGNT
                     FVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIANVLNCASGLLGPIL
                     SAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSYVIYVVRKRAVGKD
                     IFIKHYHIVVESWGLLFVQAIIEIYFK"
BASE COUNT          291 a          114 c          176 g          332 t
ORIGIN      
        1 aatttctaat acaatttgct aaagggttag aaaaagttaa agctatgggc atagcaggta
       61 ttatcagcac aattgtaaca ataattgcca atattttctt cttgcttatt ctgaaatggg
      121 gattaattgg ttttttcatt gcaaatattt tatcacagat tatttcagct acttatctgt
      181 cagtattagt gagaatatgg gaatatatcg attttgaggc taaagatcct cagctaaaga
      241 gagaaatgct aaattactcg attccattaa ttgcaactgt tgttggatgg tggattaata
      301 gtacagctga taggtatgtg gtttccttta tagtaggtgt ctcggcgaat ggcttattgt
      361 ctgtggcata taaaatacct caaataatta atactttgca gggaatattt atacaggcct
      421 ggcaaatttc tgcaattaaa gaatatggag ataatgagac tgcaaaattt tatggaaata
      481 catttgttat gattaatttg ttaatgtgca taacctgttc aagtcttatc atattaacta
      541 gaccgttttc acatattctt tatgcgaaag acttttatga agcgtggaaa tatgttccat
      601 ttttattaat agctaatgtt ttaaattgtg catcaggtct gttaggacct atcttatcag
      661 caaaaaaaga ttctaaatcc atgatgtggt ctgcaatcat aggggcatta gttaatgtga
      721 ttatgagtgt agtccttgtt tataagatag gggttcaagg tgccacaata gccactgtta
      781 tatgttcata tgtaatatat gttgttcgta aacgagctgt gggaaaagat atttttataa
      841 agcattatca tattgttgtg gaatcttggg gattattgtt tgttcaggca atcatagaaa
      901 tatattttaa aaa
//
LOCUS       DAZUKZ010000268          809 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000268 DAZUKZ010000000
VERSION     DAZUKZ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.278"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACG8EF_002970"
     CDS             136..309
                     /locus_tag="ACG8EF_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948190.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          272 a          130 c          102 g          305 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaag
//
LOCUS       DAZUKZ010000269          795 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000269 DAZUKZ010000000
VERSION     DAZUKZ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.279"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>795
                     /locus_tag="ACG8EF_002971"
     CDS             <1..>795
                     /locus_tag="ACG8EF_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFS4948191.1"
                     /translation="EILINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGFN
                     SGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQKG
                     ITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIPL
                     KRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVVT
                     DGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
BASE COUNT          270 a          117 c          148 g          260 t
ORIGIN      
        1 tggaaatact aatcaataaa ggtgatttgg atgatttgtt tcgtcgtcac ctagaattgg
       61 ctattaacgc tttattacaa gctgaattaa cggtatttct tgactatgaa aagtatgata
      121 gagctggatt taattcaggt aattcccgta atgggaatta ttcgcgctct tttaaaacag
      181 agtacggtga attgaattta gtaataccta gagataggaa tggagacttt tctcaacaaa
      241 ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt cagctagttc
      301 aaaaaggaat tactatgtcc gaaatctctg atctaatcga aaaaatgtat ggtcattatt
      361 acaaaccaca aactatttca aatatgagta aaattgtgtc tgaagatgtt tcggcattta
      421 aagaaagaac tttagaagct aaatactcag tcatttttat ggatgctact catattccct
      481 taaaaagaca aaccgtatca aaagaagctg tctatatagt aattggtatc aggttggatg
      541 gaactaaaga ggttctagga tttactatag ctccaactga atctgcttat gtttggaaag
      601 agattcttca agatctaaaa gaccgtggtt tagaagaggt tttgttagtc gtaacagatg
      661 gtttaagtgg tattgacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat
      721 gttgtgtcca tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa
      781 tctgtagcga tttca
//
LOCUS       DAZUKZ010000270          792 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000270 DAZUKZ010000000
VERSION     DAZUKZ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.280"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>792
                     /locus_tag="ACG8EF_002972"
     CDS             <1..>792
                     /locus_tag="ACG8EF_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4948192.1"
                     /translation="IFQLWIGGIFLIDVILLAKGTISITNFVIVFMYANMVPTLWTTL
                     ISIIWKWQDYSVSLNRYYEYYSNNNKVAGTKKINKINSITGQSLNFSYNKRKVLSNIS
                     FSTEMNKLFMISGENGTGKTTLMKLFTKQLPVSNNQLFVNGIDINMIDNESLRRLISI
                     ATQIPYIINSSVKENILMGTKNYDKKKFSKLIELLGISKFMTKLGITLDDNIYDEKIL
                     LSGGEKQKISLARALIKSASVIILDEPFRNLDNETRDNLRDYILNT"
BASE COUNT          328 a           85 c          122 g          257 t
ORIGIN      
        1 tattttccaa ttatggattg gaggaatatt tcttattgat gttattttat tagctaaagg
       61 aacaataagt attactaatt ttgtaatagt atttatgtac gctaacatgg tccctacttt
      121 atggactact ttaattagta ttatttggaa atggcaagac tattcagtgt cattaaatag
      181 atattacgaa tattattcaa ataacaacaa agtggctggc acaaaaaaaa taaataaaat
      241 aaattctatt actggtcaat cattaaattt ttcatataac aaaagaaagg tattaagcaa
      301 tataagtttt tcaactgaaa tgaataaact attcatgata tcaggagaaa atggaacagg
      361 gaaaacaaca ctgatgaaat tatttactaa acagttgccg gttagtaata atcaattatt
      421 cgtcaacggg atagatatta atatgattga taacgaatca ttaagaaggt taatttcaat
      481 cgcaacacaa ataccatata taattaatag ctccgtgaag gagaatattt taatggggac
      541 taaaaactat gataaaaaaa aattcagtaa attaatagaa ctattgggaa taagtaaatt
      601 tatgacgaaa ttaggtatta ctttggacga caatatttat gatgaaaaaa tattattatc
      661 tggaggagaa aaacaaaaaa taagtttagc tagggcgctt attaaatcag cttcagttat
      721 tatattagat gaaccattta gaaatttaga taatgaaact cgcgataatt tgagggatta
      781 tatactaaat ac
//
LOCUS       DAZUKZ010000271          770 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000271 DAZUKZ010000000
VERSION     DAZUKZ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.281"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>770)
                     /locus_tag="ACG8EF_002973"
     CDS             complement(109..>770)
                     /locus_tag="ACG8EF_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFS4948193.1"
                     /translation="RIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQ
                     PLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLT
                     DVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQY
                     QFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEA
                     A"
BASE COUNT          213 a          177 c          148 g          232 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgattcggc
//
LOCUS       DAZUKZ010000272          763 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000272 DAZUKZ010000000
VERSION     DAZUKZ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.282"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(9..521)
                     /locus_tag="ACG8EF_002974"
     CDS             complement(9..521)
                     /locus_tag="ACG8EF_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948194.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            complement(528..>763)
                     /locus_tag="ACG8EF_002975"
     CDS             complement(528..>763)
                     /locus_tag="ACG8EF_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFS4948195.1"
                     /translation="FNSSTEFKLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGT
                     YIKFLENYNDLKSTNNYQFGNLSYEMISKVLSL"
BASE COUNT          259 a          110 c           74 g          320 t
ORIGIN      
        1 taacatcctt aatatcttaa tttatccata ttttgagaaa taatactttt ccattcagaa
       61 actaatttag taaggaatct tgcaatgttt tgtctcatta attcattatt tctattatct
      121 ataagatctg ttagatattt ttcaaattct aaaaagctat ccgaattttc ttcgacgatt
      181 aaatacttaa gagaatttat atcggaaaag atagattcaa gtttactaat ctcattttct
      241 aaatctatat tttcacgtgt gattatctca tcactttttc ttaaaaagct aaaaattgca
      301 tcagttgctt tacacttttc cattaaagaa gtatattctt caatttttct tctttcttta
      361 tggtcatttt ttaaatcagt ttcaatgtct tttttcattt gttctatact gagtttgttc
      421 tttttgttct tttgagtaaa tctaaaattc aaacacgaaa aaagtatgtt agcaataaca
      481 cctataatag caaataaatc atttatacta tttaatttca tttatcatca caaacttaga
      541 actttactta tcatctcata agacaagttt ccaaattgat aattattggt agattttaaa
      601 tcattataat tttcgagaaa ttttatataa gtacctctca tcggtttatg gctattaaaa
      661 gtgttaaaat cactatcttc aactatttct tgaaacctat ttaatttttc aataaaaaat
      721 tgtgcataaa gtgctaactt aaattcggta gagctattaa agt
//
LOCUS       DAZUKZ010000273          753 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000273 DAZUKZ010000000
VERSION     DAZUKZ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.283"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..734
                     /locus_tag="ACG8EF_002976"
     CDS             <1..734
                     /locus_tag="ACG8EF_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFS4948196.1"
                     /translation="DKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRK
                     LMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKI
                     YLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQ
                     YQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIR
                     WYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          321 a           97 c          127 g          208 t
ORIGIN      
        1 cagataaata tagcaagata aagcaagaaa ttacagcgat tgtaaaagaa tcaagaaact
       61 cttatggtta tcgtagagtc actttagcgt taaagatgaa aggatataca atcaatcata
      121 aaacagttag aaaattaatg tcccaaatgg gacttacctg tcaaatccga ataaagcgat
      181 ataaatctta taaaggaaca gtaggaaaaa ttgccaagaa tgtgttaaaa cgaaattttt
      241 cagtagatac acccaataaa aaatgggtga cagacgtcac tgaattcaag ataaaaggaa
      301 gaaaaatcta tctatctcct atacttgatt tatttaacgg agaaataatt agttatagta
      361 tatcaactag tccaacatat aaactgattg aagaaatgct tcaacaagca attaaaaaaa
      421 agggaactga gggttcactg attctacatt cagatcaagg atggcagtat cagatgccac
      481 aatatcaaaa aaaattaaaa gagaataaca ttattcaaag catgtctaga aaaggaaatt
      541 gcttagataa ttctgtaata gaaaattttt ttggtgtgct taaatcagag tttttttatc
      601 gagaaaaatt ccgatcaatt gagatatttc aaagtaaatt aaatgagtat attaggtggt
      661 ataataacaa aaggataaaa ctaaagttaa atggactatc tccagtagaa tatcggaaac
      721 agtccattaa atagttcaac ttttggggtt cag
//
LOCUS       DAZUKZ010000274          749 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000274 DAZUKZ010000000
VERSION     DAZUKZ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.284"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..498)
                     /locus_tag="ACG8EF_002977"
     CDS             complement(73..498)
                     /locus_tag="ACG8EF_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4948197.1"
                     /translation="MHLHRISALAVQIGRYHHSETRAVMEGLEEAIQTTNDCPYRRTF
                     HSDQGWSYQMKAYRNKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCF
                     EELKSAIDSYIYYYNNERIKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          215 a          138 c          152 g          244 t
ORIGIN      
        1 gtgaccccca aaagttagac ttttttggag tggagaaatc cactcttttt ttcatgctct
       61 aaaatgatat ttttacacaa ccgtaacggt tgttttacga aattggacgg gactttgcca
      121 gtttaatttc tgttttatcc gttcattatt gtagtaatag atatagctat cgatggctga
      181 ttttaattcc tcgaaacagt tataaacttt cccgtgaaaa atctcttgtt ttaataagcc
      241 aaaaaaattc tccataagtg aattatctaa gcagttccct ttccgagaca tgctttgaaa
      301 gattttgtac tccttcaatt tgtttctata agctttcatt tgatacgacc aaccttgatc
      361 tgagtgaaac gtacgacgat agggacaatc gttagtggtc tggatagctt cctccaaacc
      421 ttccattacc gcacgtgttt ctgaatggtg gtagcgaccg atttgaacgg ctagggcgga
      481 aatacggtgc aaatgcacgg tggtttcatg caatgcttac agggcgtatt ttgtaagggt
      541 atgtaacgga gataatcccg acggtaatgg atgtgagagg ccaagccgac gctgacggta
      601 gggcaaacaa acatagttgc actctagcga tcgttattta tttttaacgg tcaaactagg
      661 gtacaactat gcttaaaaca cttcgcctca acttctggta gcaaaagtga ttttgaaagt
      721 caaaatattt tttatgagtt atttataat
//
LOCUS       DAZUKZ010000275          746 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000275 DAZUKZ010000000
VERSION     DAZUKZ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.285"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            170..>746
                     /locus_tag="ACG8EF_002978"
     CDS             170..>746
                     /locus_tag="ACG8EF_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS4948198.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTL"
BASE COUNT          257 a          109 c          122 g          258 t
ORIGIN      
        1 tttattagaa ttacgcctaa tttgtgtctc tcagggcaat tctaagaacg tttcaagaga
       61 tagcgacact atcgttatcg aaaaaggcga aaacctatct cattttctgc taatgtaagt
      121 tggtttagct tttgttaata tgactttatg aaaatggagg taattaaata tgatttttcc
      181 taaagttaat gaagcaagct tgaataaagg atcctttttt catatctatg aaaacgaaga
      241 ttttgaactg tataatatcc tttcacaaaa agaggtcatc gaaaaccaaa taatctgcca
      301 tccaattatt tacaaatcta ttttcaagta tgcatttttt gcttccaatg attttcaaag
      361 aatggagatt accgactgta tattcgaaaa ctgtgatttt tcaggctgta tattgacaaa
      421 aagcatgttg catcgcgtga aatttgtcaa ttgtaaatta acaggtacta aattgatgga
      481 aagttatatt gggaacacgt tattcgagaa ttgtaaaatg gactatgtaa atttatctgg
      541 ttcaaacata aaagaaagta cctttgagca ctctgtctta tcaagcgcag attttgtaga
      601 ctgttccctc actaaaataa tgttttctac taatgattta gaaaatgcta gcttttttga
      661 tactgattta aaaaatatcg atttaagtag caactccttt gaaatgattg aagtacgctc
      721 ggaacatcta aaaaattgta cgctta
//
LOCUS       DAZUKZ010000276          712 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000276 DAZUKZ010000000
VERSION     DAZUKZ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.286"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..446)
                     /locus_tag="ACG8EF_002979"
     CDS             complement(<1..446)
                     /locus_tag="ACG8EF_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4948199.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DAZUKZ010000277          707 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000277 DAZUKZ010000000
VERSION     DAZUKZ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.287"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACG8EF_002980"
     CDS             132..344
                     /locus_tag="ACG8EF_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948200.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>707
                     /locus_tag="ACG8EF_002981"
     CDS             506..>707
                     /locus_tag="ACG8EF_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFS4948201.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNM"
BASE COUNT          253 a          104 c          123 g          227 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgt
//
LOCUS       DAZUKZ010000278          703 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000278 DAZUKZ010000000
VERSION     DAZUKZ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.288"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          297 a          106 c          122 g          178 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa
      361 caaacgataa gttatgataa atgagttgcc gaaagatttc gaaaaacgaa atcgaaaaaa
      421 acttttaaaa aagagttgac aatcgcttgt ctctctgata tgatataaaa gttgctacgg
      481 cagccaaaaa tgaatcggga aacactctcg atgaagtaga cctttgaaaa ctgaacaaag
      541 taagacaaac caaatgtgta gggcgtcttg attcaattca agacaacaaa catttttaac
      601 aagcaagcaa tatgctagca aacaaattga gcttaacaat cgcaagattg ttcgaacttt
      661 ttatgagagt ttgatcctgg ctcaggacga acgctggcgg cgt
//
LOCUS       DAZUKZ010000279          690 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000279 DAZUKZ010000000
VERSION     DAZUKZ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.289"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..654
                     /locus_tag="ACG8EF_002982"
     CDS             <1..654
                     /locus_tag="ACG8EF_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4948202.1"
                     /translation="GLMLVIFSGKQYADNILLWGGEDNIEKINNNLTILDTALENKEQ
                     KISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKTNLENQLSAKDSEL
                     QGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQSTINSLNQQINQMLS
                     EKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTANKAQQIVNEHTIQ"
BASE COUNT          324 a           86 c          104 g          176 t
ORIGIN      
        1 ggattgatgt tagtaatttt ctccggaaag caatatgctg ataatatttt attatgggga
       61 ggggaagata atattgaaaa gatcaacaac aatttaacta tattagacac agctctagaa
      121 aataaagagc aaaaaattag tattttaaat tcacaattgt cttcaaacaa ccaaactctt
      181 gaacaactaa gaactaatat tgaagtttcc aaacaaaaag tggcatcttt agaaaacgac
      241 aagaatcaat tagtatcaga gaaaactaat ttagaaaatc aattgtcagc aaaagactct
      301 gaattgcaag gaaaacaaaa tgaaattaat gcgaaaatag acgaaattaa ccaaaaagta
      361 gctgaaataa atcgattaaa tagcgattgg aataatcgtt tacaaaatga aaagaacaaa
      421 tttaatgaag aacaatctac tattaatagt ttaaaccagc aaattaatca aatgttgagt
      481 gaaaaaaata acattcaaaa tcaactgcaa gctactcagc aaaagctaga agaagaaaag
      541 tcaaaaaata gtagtttgaa taattacatt gaaaagcttc aaaaagctaa aaatgatgta
      601 gaggataccg caaataaagc acaacaaatt gtaaatgaac atacaatcca atagatatag
      661 ttttaattta acaaatggta tgtttggaga
//
LOCUS       DAZUKZ010000280          683 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000280 DAZUKZ010000000
VERSION     DAZUKZ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.290"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..>683
                     /locus_tag="ACG8EF_002983"
     CDS             106..>683
                     /locus_tag="ACG8EF_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4948203.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILS"
BASE COUNT          271 a           65 c          147 g          200 t
ORIGIN      
        1 ttgacatgga aacaatcttt taaaaagcaa aaagatgata aagttacttt ttatgggatg
       61 ttggtagaaa acaaattatt ataaatatta gaggtataaa aagttatgaa acacaaggta
      121 agtgtgatag taccaattta caatgttgaa aaatatttaa gaaaatgtgt ggatagttta
      181 ttgagtcaga cattaaaaga gatagaaatc gtattagttg atgatggttc gccagatgct
      241 tcgggggaaa tagcagatga gtatcaaaaa aaatattcta atgtaaaaac aatacataga
      301 gaaaatgggg ggttaggacc ggcaagaaat acaggaattg agaatgcgac aggagaatat
      361 gtggcttttc ttgattcaga tgattgggta gaaagtgata tgtatgaaaa attatactta
      421 gttgcgtcca aagaaaatgc tgatatagta gttagtggac attgtgattt tgcgaatgac
      481 aaagctatgg tgaaaaagca acatcctttg agtgggaatg tatatgatac gcctaatagt
      541 attaaaaatg tacgaaaaaa tttatttgga catagtccaa aagatcaaga agtagaggcc
      601 ttcccgatgt cagtatgtat gtctttgtat aataaaaatc tgctagataa atatagacta
      661 agatttgaaa aaattttatc tga
//
LOCUS       DAZUKZ010000281          662 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000281 DAZUKZ010000000
VERSION     DAZUKZ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.291"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..457
                     /locus_tag="ACG8EF_002984"
                     /pseudo
     CDS             122..457
                     /locus_tag="ACG8EF_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            553..>662
                     /locus_tag="ACG8EF_002985"
     CDS             553..>662
                     /locus_tag="ACG8EF_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4948204.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT          234 a          127 c          132 g          169 t
ORIGIN      
        1 tttatataat aaaatgcaat acttttcgag agtgtaaaat attttgtgta aatagaaaaa
       61 aggaagtccc ttctgtagaa tagagttacc acaacacatt cacagaaaag aggacttcca
      121 tatgaacgat tttactacag aaattctaaa gactctagcg aacaaaggcg atttgaatga
      181 attattccgt gtccatttgg aaaaagctgt caatacgctt ctcaaaacgg agttaacggc
      241 tttcctcgat tacgaaaagt acgatcgcat tggttttaac acgggtaatt ctcgtaacgg
      301 ctcctatgac cgtacggtca agaccgagta cggggaactt catctccaga ttccgcgcga
      361 ccgcaacggc gagttcaagc aacagactgt tcctgcttat agacggacga atgatacctt
      421 agaggagacc gtcattcacc tcttccgaaa aggtatggat aggctacaat taactagtgg
      481 ataggctaca attaactaga cagaaaaatt aaggtgtgta gactagaaga aaacatacca
      541 ggaggaattt ttatgtctaa gagaacacga agaacttttt cacaagaatt caagcaacaa
      601 atcgtcaatc tttacttagc tggaaagcca cgtgtagaaa tcattcgaga atatgaacta
      661 ac
//
LOCUS       DAZUKZ010000282          656 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000282 DAZUKZ010000000
VERSION     DAZUKZ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.292"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            194..592
                     /gene="spxA"
                     /locus_tag="ACG8EF_002986"
     CDS             194..592
                     /gene="spxA"
                     /locus_tag="ACG8EF_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFS4948205.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          231 a           98 c          128 g          199 t
ORIGIN      
        1 tttaaatgat aaaaaaagcg agaatgctgg ttttactgta tattaacctt agataacttt
       61 cattttttta aaaaaattat gaaaaaaagg tagctattta aaagttgatt atgtataata
      121 gaaacagatg ggagattatt atgatcttct gttacaaaaa agtgattctt atcacgaaaa
      181 ggagtggtgc acgttgttaa cattatatac ttccccaagt tgtacttcct gcagaaaggc
      241 tcgtgcgtgg ttgcaagaac atcagattcc atttgttgaa cgtaatattt tttcagaacc
      301 attgaacagt tctgaattaa aagcgatttt acaaatgaca gaagacggaa cagaagaaat
      361 catctcgaca cgttccaaag tattccaaaa actgaacatg gatttagatg atctgccgtt
      421 acaagaatta ttggaacttg tgcaaaacaa tcctggattg cttcgccgtc caatcatgat
      481 tgatgataaa cgtctgcaag ttggttttaa tgaagatgaa attcgtcggt tcctaccgcg
      541 cgatgtacga caattagaat tacgtcaagc acaattgatg gctggattat agaaggataa
      601 tacgaaactt ggaaaagact ggctctttgt caaatgatgt tggtaaagag aaatct
//
LOCUS       DAZUKZ010000283          652 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000283 DAZUKZ010000000
VERSION     DAZUKZ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.293"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>652
                     /locus_tag="ACG8EF_002987"
     CDS             <1..>652
                     /locus_tag="ACG8EF_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS4948206.1"
                     /translation="SNVARNIASNIRAIYVDEFQDTRELQYNVIAKLLQNNPQIQSMF
                     VGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCYRSNQRLVDFYSNFQSCPYE
                     IESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIESGIHEKDICVLAPQHYPLFS
                     LGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFTESGEKVRRRKLIANDILI"
BASE COUNT          242 a           91 c          115 g          204 t
ORIGIN      
        1 cagtaacgtt gcacgtaata ttgctagtaa tataagggcg atttatgtgg atgaatttca
       61 agatactaga gaattacaat acaatgtaat agctaaatta cttcaaaaca accctcaaat
      121 tcagtcaatg tttgttggag atattgacca agcaatttat ggatcattag atggtatagc
      181 taaatcagtt gaagaactag aaatgctaac tagtcacact tttcaatcaa agacgctaca
      241 tggctgttac aggtctaatc agagattagt agacttctac tctaattttc agagttgtcc
      301 ttacgagatt gaaagcagag ggaataataa aaacgaccgt ggttttataa gttatgattg
      361 caaagttact aaggaggatt tacccaacat aataaaaaat ataataaagg aaaagattga
      421 aagcggcatt catgaaaaag atatttgtgt acttgctcct caacactatc ctctgttttc
      481 actgggtgag tatttaaaga aagaattacc gtattgcaat tttgatgcta taaatatatc
      541 accaattaag gtaaataatc atagtttatt tttcaagtta gcaattttgt actttactga
      601 atcaggtgaa aaggttcgaa gaagaaagct aattgcaaac gatattttaa tt
//
LOCUS       DAZUKZ010000284          642 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000284 DAZUKZ010000000
VERSION     DAZUKZ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.294"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..281
                     /locus_tag="ACG8EF_002988"
     CDS             78..281
                     /locus_tag="ACG8EF_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HFS4948207.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            281..631
                     /locus_tag="ACG8EF_002989"
     CDS             281..631
                     /locus_tag="ACG8EF_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4948208.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          265 a           66 c          113 g          198 t
ORIGIN      
        1 gatatttaat attacaagca atcttaagaa attcgaaaag gaaaaaatga ttatgaaaaa
       61 gaaagaaaaa gaaagtattg aaacattgtg ttattctagg aatattagga acttgtctag
      121 ctggcattgg tacaggaata gacgttgacg cagctactta ctatggaaat ggtctttatt
      181 gtaacaaaga aaaatgttgg gtaaattggg gacaatcatg gtcagaaggg ttaaaacgtt
      241 ggggagataa tctttttggt agttttagtg gaggacgata atgacttcaa taaacattga
      301 tcacacaaaa aaagtcatac atgaattata caatagttta atgacacgac ctaatcagac
      361 gaaagcatta ttagatatta ctgatgtttt atttcaggta tataaaaaaa ttgatacagc
      421 aaaatatcct gaaattcttg taaataaatt agttaattat atttacatcg tgggttttga
      481 taataaaata cgctttattg ataatgatga aaaattatta atagaattag gggatatttc
      541 taaaaaagct ggaattaata gtaaatacaa agcaaacttt acagataaat cgcaatttta
      601 tagttattca gaaacaatgc ctcaacgata aaatatagta ta
//
LOCUS       DAZUKZ010000285          600 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000285 DAZUKZ010000000
VERSION     DAZUKZ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.295"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..259
                     /locus_tag="ACG8EF_002990"
     CDS             <1..259
                     /locus_tag="ACG8EF_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFS4948209.1"
                     /translation="TLNKIKKYDISVDFQSKQKCKIKFVPTIVAYSKNKEVGRIDNEL
                     DNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYGED"
     gene            246..>600
                     /locus_tag="ACG8EF_002991"
     CDS             246..>600
                     /locus_tag="ACG8EF_002991"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948210.1"
                     /translation="MEKIKQTFPLINKYWNKKKIFIFFITFLLSSLFSLIFPYLFSNV
                     IDLYLENELSQNIVRIVTILIFQLFLVLIKEVYGYIKTIYAFEVDLSMKIDFLKVLQK
                     KNLFFSQTKHSGEIQF"
BASE COUNT          237 a           52 c           93 g          218 t
ORIGIN      
        1 aactttgaat aagataaaga aatatgatat atctgtagat tttcaatcta aacaaaagtg
       61 taaaattaag tttgttccga ctattgttgc atatagtaag aataaagaag tggggagaat
      121 tgacaatgaa ttagataatt tgtatcgaaa aaagcaagat ggttcaattg taatagacaa
      181 gaaaaaaaca aagacattgc ttatagattg gatgaaaaaa atggataaac aaggagaata
      241 tttgtatgga gaagattaaa cagacatttc cgttaattaa taaatattgg aataagaaaa
      301 aaatttttat attttttatt acgtttctgc tgtcatcttt attttctcta attttcccgt
      361 atctatttag taatgttatt gatttatatt tagagaatga gttatcacag aatattgtgc
      421 gaatagtaac gattcttata tttcagttat ttttagtctt aattaaagaa gtgtatggat
      481 atataaaaac tatttatgca tttgaagttg atttatcaat gaaaattgat tttcttaagg
      541 ttttacaaaa aaaaaatctt tttttttcgc aaacgaagca ttcaggagaa attcaattta
//
LOCUS       DAZUKZ010000286          591 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000286 DAZUKZ010000000
VERSION     DAZUKZ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.296"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..>591
                     /locus_tag="ACG8EF_002992"
     CDS             26..>591
                     /locus_tag="ACG8EF_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFS4948211.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT          210 a          101 c          117 g          163 t
ORIGIN      
        1 gaaaaagata gcaggaggaa tgacgatgaa tcattttaaa ggaaagcaat ttcagcagga
       61 tgtgattatt gtagccgtgg gctactatct tcgttataac cttagctatc gtgaagttca
      121 agaaatctta tatgatcgtg gcattaacgt ttctcatacg acgatttatc gttgggtgca
      181 agaatatggc aaactactct atcaaatttg gaaaaagaaa aataaaaaat ccttttattc
      241 atggaaaatg gatgaaacgt acatcaaaat taaaggaaaa tggcattatt tgtatcgagc
      301 catcgatgca gatggtttaa ccttggatat ttggttacgt aaaaaacggg acacacaagc
      361 agcctatgct tttcttaagc ggttagtgaa gcagtttgat gaaccgaagg ttgtagtcac
      421 agataaagcc ccctctatta caagtgcctt taagaaacta aaagaatacg gcttttatca
      481 agggacagaa catcgtacca ttaaatacct gaataatttg attgaacaag accatcgtcc
      541 agtaaagaga cgcaataaat tctatcgaag tttacgcact gcctctacca c
//
LOCUS       DAZUKZ010000287          588 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000287 DAZUKZ010000000
VERSION     DAZUKZ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.297"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..150)
                     /locus_tag="ACG8EF_002993"
     tRNA            complement(65..150)
                     /locus_tag="ACG8EF_002993"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="ACG8EF_002994"
     tRNA            complement(167..238)
                     /locus_tag="ACG8EF_002994"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="ACG8EF_002995"
     tRNA            complement(250..322)
                     /locus_tag="ACG8EF_002995"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="ACG8EF_002996"
     tRNA            complement(340..421)
                     /locus_tag="ACG8EF_002996"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="ACG8EF_002997"
     tRNA            complement(429..501)
                     /locus_tag="ACG8EF_002997"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="ACG8EF_002998"
     tRNA            complement(505..577)
                     /locus_tag="ACG8EF_002998"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAZUKZ010000288          585 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000288 DAZUKZ010000000
VERSION     DAZUKZ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.298"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(279..351)
                     /locus_tag="ACG8EF_002999"
     tRNA            complement(279..351)
                     /locus_tag="ACG8EF_002999"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(316..318),aa:Lys,seq:ctt)
     gene            427..>585
                     /locus_tag="ACG8EF_003000"
     CDS             427..>585
                     /locus_tag="ACG8EF_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4948212.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKI"
BASE COUNT          213 a           82 c           93 g          197 t
ORIGIN      
        1 actttttagt ttttttatag tactcaaatt taatcctgta tgtttagaga tctcatttgc
       61 agtatggttt tgtaaagttt ctcggattat tgttatatta gcaatttcat tacgcataat
      121 tatgttcctt tcttatcaaa aatatcaatg actacagtgg gcttttagtg aaaaaaaggg
      181 gtactacagt ctcttttgaa aacaaaaaaa tacagtatat acgttttcta aatgctaagc
      241 accaacgttt acactgtatt taagggtttg gcacccaatg ggctgtgagg ggctcgaacc
      301 cgcgacccga tgattaagag tcatctgctc taccaactga gctaacagcc caaattaata
      361 ctttgttatt ctaacaccta aaataaaaaa gtgcaatact tattatcaga attgagagtg
      421 aattttatgg attttcaaga ttatgaaaaa atatttgaaa gttatccatc gatattgaaa
      481 attactgatt taactaaaat gttaaatatt ggaaaaaata aagcttataa tttgctgaaa
      541 gatagatcaa tagaaaatat aaaaattgga gcaaggtaca aaata
//
LOCUS       DAZUKZ010000289          555 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000289 DAZUKZ010000000
VERSION     DAZUKZ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.299"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>555
                     /locus_tag="ACG8EF_003001"
     CDS             <1..>555
                     /locus_tag="ACG8EF_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948213.1"
                     /translation="KFANSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLL
                     VNTVVELAKWAKSNETYSQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENS
                     QWIKELKREDFIETLRILKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEK
                     RIDNIINYINSLSEPLKCRGLDFI"
BASE COUNT          225 a           58 c          101 g          171 t
ORIGIN      
        1 ctaaatttgc aaactcattc tggtttttaa aaaatattaa taaaacagtg gaaaagaatg
       61 gagtttataa aggtgatttt cattttcaaa atgctgagac tattgaacga gaacatcatt
      121 atttcgattt attagtgaat acagtggtag aattagcgaa atgggcaaag tcaaacgaaa
      181 cttatagtca agtacttaaa agaattatga gtcaagaaac ttcatttatc tatgagttaa
      241 gcaagtcaaa tttgaaatat acttataatg aagatgaact tgaaatttta gataatccag
      301 aaaattcaca atggattaaa gagttgaaga gagaagactt tattgagaca ctaagaattc
      361 taaaatcgtt atttgatttg aaattagcca aagctaccat catgggaatt gaaagagatt
      421 catattattg ggaggaaatt ggactatatg ctccccagat tgaaggtgaa gataaagaga
      481 aaaggataga caacattatt aactatatta atagtttaag tgaaccttta aaatgtagag
      541 gacttgattt tatag
//
LOCUS       DAZUKZ010000290          551 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000290 DAZUKZ010000000
VERSION     DAZUKZ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.300"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>551
                     /locus_tag="ACG8EF_003002"
     CDS             <1..>551
                     /locus_tag="ACG8EF_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948214.1"
                     /translation="IGYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSF
                     RSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAM
                     TMVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPY
                     VDILQKRAIYLGKEFLIGSIDSE"
BASE COUNT          177 a           76 c           86 g          212 t
ORIGIN      
        1 aataggctat aacctttttc tttatattgt tacgctcatt actctattta tatgtcatta
       61 tgtaatgtat gaaattacca aaaaacatgt tactagctcc tttttcgcta ttatatatac
      121 aacatcttct tttaggagtg tagaaatatt tcttcgaggg gcaatggggg aattactcgc
      181 aatgtcaata cttcctctta ttttactcgg gtttataaag ctttacgact caaaaaaaga
      241 atcttgggtt atgttggcga ttagcatgac tttattaatt tacactcatg ttctttctgt
      301 agctatgact atggtaatga ttataattgc tttaattatt caattttatc ggaaaaagaa
      361 aatagaaatc ttcttaatta ttaaattaat aaaagctgct atagttactt tacttctttc
      421 actagcgttt ataggtccta tgattgagca gacattgtat caagatctga atagacctta
      481 tgtggatata ttacaaaaaa gagcaattta tctgggtaaa gagtttttga ttggaagtat
      541 agattcagaa t
//
LOCUS       DAZUKZ010000291          544 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000291 DAZUKZ010000000
VERSION     DAZUKZ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.301"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..360
                     /locus_tag="ACG8EF_003003"
     CDS             <1..360
                     /locus_tag="ACG8EF_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS4948215.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          202 a           89 c          116 g          137 t
ORIGIN      
        1 cgaaaccaac cagttgttaa accaaatgca atgcaaatca ataactcatt gaagcgatgc
       61 tttgtgaaag cattggctct acacggactg ggattatatg tatttcaagg ggaagatatt
      121 ccaacaccac ctagaatcga tacaaagaaa ttaaacatgc tagagacgat tctagaagct
      181 ttcaatgagc agatgggtaa agatatgacc aaaaccttaa tcgaatatgt taatgagcag
      241 acagataaat tagggctctt agctgataac gttgaaacta ttgaacagtt aagctatgag
      301 caatgtgcct tgatggagcg agcaatagca gctaagagaa aagaattaga taagaagtga
      361 tatgagtgtt taaaccatta atcgattcat attcagcggt tctgaaaaag ttcaaaggaa
      421 aagacataag cgcaaccatc aatgaggaag tgaacattga tcgactaaag acgatgtatg
      481 acggctacga tggtgatcga gtcattgaaa ttcgttttat tgatcctaga cgtttcaccg
      541 taca
//
LOCUS       DAZUKZ010000292          526 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000292 DAZUKZ010000000
VERSION     DAZUKZ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.302"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACG8EF_003004"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACG8EF_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS4948216.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DAZUKZ010000293          519 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000293 DAZUKZ010000000
VERSION     DAZUKZ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.303"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(119..418)
                     /locus_tag="ACG8EF_003005"
     CDS             complement(119..418)
                     /locus_tag="ACG8EF_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4948217.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          161 a          101 c           62 g          195 t
ORIGIN      
        1 gaaacatctg tttctttatt tagaataaaa aaatacagac tctgttcaaa taaatgaacc
       61 tagcctgcat tatgagattg actatacttt ttaattcatt gagatgaata actcgttttt
      121 ataaatgccc aaaagtaatc ggtgccgaag ccattcctcg acctaattct ttttcaaatt
      181 ttcctattgt tgatatctcc atataaaacg gtgatactgg tttagacaat tctcccctga
      241 ttgctaacgg acgtaaacta cgttccaaat tatttatgac tttaggataa tactgacctt
      301 tttctaagcc tgtctttgcg tctatcaaaa gtatacgttc cttttctttg atctctggat
      361 ttaaaacgag atcatataat tttagtacca tgttttctat ttcttcactt ttagacatca
      421 cgatttcctc ccaaaaatat atttcttccc actacattct ataaacaaag tatacgctac
      481 atctagattt ttttaaatca ttactggatt gttaaaacc
//
LOCUS       DAZUKZ010000294          499 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000294 DAZUKZ010000000
VERSION     DAZUKZ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.304"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..467)
                     /locus_tag="ACG8EF_003006"
     CDS             complement(<1..467)
                     /locus_tag="ACG8EF_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4948218.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYLKKLETLI"
BASE COUNT          142 a           97 c           64 g          196 t
ORIGIN      
        1 tgaatcaagg tttctaactt ttttaaatat gcgttctctg ctcttaaacg aataacttct
       61 tcttcaagag actcatcttt aagtttttta ggaatgttta gcttggaatt catactaatt
      121 tttctgcccc ttttttggct ctcaagtgaa gaagcaccgc cttcttcata ttgctctatc
      181 catttactta gagttctatt tgaaccgata ttaaattttt tagcagtttc ttggatagaa
      241 agaccatttg tttccatata ttctataaca tcaagtttaa attttgtagt gtagcttttg
      301 ccacctccaa ccaagccttc ccaaccatga tagttataaa tccttaccca atgtctaacc
      361 agtgtacgat ttatttgata tttatgtgca agatatttgt agccgccttc gttatttaaa
      421 tagtctgaaa ctactttttt cttaaaaaca aatgtatatt tccgcaaaaa aagcacccct
      481 tttaattaga tttctagtc
//
LOCUS       DAZUKZ010000295          498 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000295 DAZUKZ010000000
VERSION     DAZUKZ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.305"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>498)
                     /locus_tag="ACG8EF_003007"
     CDS             complement(<1..>498)
                     /locus_tag="ACG8EF_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4948219.1"
                     /translation="GNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVSAAI
                     PIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGYVVF
                     QIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQVFD
                     HFVFGV"
BASE COUNT          222 a           75 c           56 g          145 t
ORIGIN      
        1 gacaccaaac acaaaatgat caaatacttg atattgatga gcaacataag aaacaaataa
       61 accaattgta agtaaataaa tcatgaatat agcagtaagt ttagaactgg actgttcaac
      121 aatcctattt atagctaatt ttctatataa ccaaataaac atcagcaaaa acaaagaatc
      181 aagcattatc tggaatacta cataaccata cccttttatc ccttcaaatg aaaacatata
      241 cgtcataacc atggaagtaa taatagatct gaaatcatca ataagcatac ataatagtaa
      301 tttatttaaa agcactatat tagttttctt cttttccttt aatataaaat aacttccgat
      361 tataggtata gctgcggaaa catcagtaaa taattcaaca aaaaaagtga gtggtatcaa
      421 taaacataca aaaagatatt ttcttttgtt aagatcagta ttagtcatat aaaataagag
      481 aaaaaagaaa aagtttcc
//
LOCUS       DAZUKZ010000296          485 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000296 DAZUKZ010000000
VERSION     DAZUKZ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.306"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..259)
                     /locus_tag="ACG8EF_003008"
     CDS             complement(62..259)
                     /locus_tag="ACG8EF_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4948220.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DAZUKZ010000297          481 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000297 DAZUKZ010000000
VERSION     DAZUKZ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.308"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          220 a           63 c           39 g          159 t
ORIGIN      
        1 gtatgaataa aatttagttt taaaattaaa gataattaag tactaccagt gtcaaaacaa
       61 aaaataaatt aagaactacc ttatgcatat aaaacatact accattacta gtaatatata
      121 ctaccttatt tcagtagtat atattatatt attcttataa gacaaggctt taggctagaa
      181 aacaatagat tatatactac taaaaaatac aataagtact accaatgtca gatatatata
      241 ataattatgt actaccatta aaattaactt atttaataag atactactgt gttaatctga
      301 aaaaaattac tttaaagtag ttacatacta cctgttaatt tagatactac cattaatact
      361 taaattagat actacctata tttatattaa gtactaccgt tctaagaata agataataaa
      421 aaaatactac caaattgcaa ttaaatacta cttaaaatgt aaaaaataag aactacccaa
      481 a
//
LOCUS       DAZUKZ010000298          479 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000298 DAZUKZ010000000
VERSION     DAZUKZ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.309"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          157 a           95 c           56 g          171 t
ORIGIN      
        1 atcactctac aataacggcg aaagcacaaa aaataaaaaa attattacgt tattaaaaaa
       61 acagatgaaa gatcaggttt tattagatct cattctttca tcacttgaag ggatttaatg
      121 aactactttt tcctctttgt cgtttttata tattcgtatt ccttcaaaca gtaggatagc
      181 ctctttcaat gcttgatatt tgaactgtcc gttacttaaa aatttagatg tgccgccctc
      241 ttcctagaac atattctttt ttactttttc attaattgac agaccaatcg aacgaaactg
      301 taaatttcga taggataatg attggtcaaa ctcattcatt ttcaagacat ctcgattcat
      361 attcaagata ggaatctttt tatcaacatc actttattcg tattttcctc taccataaaa
      421 gcttcacgct ccacaaaatt gttttataca ctactattca gcccattcaa cgaccaatg
//
LOCUS       DAZUKZ010000299          452 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000299 DAZUKZ010000000
VERSION     DAZUKZ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.310"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>452
                     /locus_tag="ACG8EF_003009"
     CDS             <1..>452
                     /locus_tag="ACG8EF_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948221.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DAZUKZ010000300          449 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000300 DAZUKZ010000000
VERSION     DAZUKZ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.311"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>449
                     /locus_tag="ACG8EF_003010"
     CDS             <1..>449
                     /locus_tag="ACG8EF_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS4948222.1"
                     /translation="ESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITA
                     DDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKE
                     KVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          182 a           67 c          105 g           95 t
ORIGIN      
        1 gaaagtggaa caacagtctt tgcagtgaaa gacggcgtaa gcttaccaga agataaactt
       61 ccagtgttga aagcaaaaga tggttataca gatgcgaaat ggccagagga agcaactcaa
      121 ccaattacag cagatgatac agaatttgta tcaagtgcaa caaaactgga tgataaatct
      181 gacgctgaca aatataatcc tgaaggtcaa aaagtgacta cagaattgaa taaggaacca
      241 gacgcatttg agggaattaa gaacaaagaa gatttaccaa aagatactaa gtatacttgg
      301 aaagaaaaag tagatgttag cgcagctgga aataaaaaag ggactgttgt agtgacatat
      361 ccagatggat catctgatga agttgaagta gatgttacag tgacagacaa tcgttctgac
      421 gccgataaat atgagccaac agtagaagg
//
LOCUS       DAZUKZ010000301          438 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000301 DAZUKZ010000000
VERSION     DAZUKZ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.313"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..122
                     /locus_tag="ACG8EF_003011"
     CDS             <1..122
                     /locus_tag="ACG8EF_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4948223.1"
                     /translation="LAALIGISGAYLSDILNGNRDGKKAQQHIETVKKILDIR"
     gene            134..382
                     /locus_tag="ACG8EF_003012"
     CDS             134..382
                     /locus_tag="ACG8EF_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948224.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
BASE COUNT          171 a           67 c           86 g          114 t
ORIGIN      
        1 aactagctgc gcttatcggt atttcaggag cttatttatc agatattcta aacggcaatc
       61 gtgatggaaa gaaagcacaa cagcatatcg aaacagtgaa aaaaatattg gacatccgat
      121 aggggggtag gcgatgacaa aactaaaaaa acaagatttt gtaaaaaaat acaattattc
      181 tccatctact tatcaacgtc gaatgtcgga actaaaaaat acagcaattt tctcagcggc
      241 gtatgaacgg gtcacaggac aagaagtttg gatcaataca gaattatacg ataaattttt
      301 gtctttcaaa tcctataaca ggttacgcac aagaaaggta acacctaaag aatttatcga
      361 gaagcattta gttgatttat aaaaaacaga acattttgag aggtgaaggt taatgggtaa
      421 attcaacaga gcattggt
//
LOCUS       DAZUKZ010000302          427 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000302 DAZUKZ010000000
VERSION     DAZUKZ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.314"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..284)
                     /locus_tag="ACG8EF_003013"
     CDS             complement(<1..284)
                     /locus_tag="ACG8EF_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4948225.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATL"
BASE COUNT          156 a           63 c           59 g          149 t
ORIGIN      
        1 agtgtagcta gagaaagtac cgatgacata aattcattta tgtcatcttt atagtcaaaa
       61 cttgcattat taattgccga atttaggctt agcccaacaa gaatcgaaaa aattgataca
      121 aatgaagtga aattattaat caaaccatat tctgcttgag tagtaatatt tacaaaaata
      181 ggaatagtta tgaatgaaat tcccttaata aaaaaattcc ctatagtata ccagaaactg
      241 gacttaacta attttgactt ggaaagtgtg ttaaataatc ccatactcta ccaactttct
      301 taattctgcc cagttttctg ggagtaaaat tttttcttct gatagacggt tataatgttc
      361 acagaaaaga acaatatttg tcaatgtttc ttttttatct tcagataatg ctgtatattc
      421 atttaaa
//
LOCUS       DAZUKZ010000303          425 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000303 DAZUKZ010000000
VERSION     DAZUKZ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.315"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          141 a           69 c           92 g          123 t
ORIGIN      
        1 aatttcatcg ggggaagagg tttggacaaa aatggaaatt tccgccaaaa gtaactgctc
       61 ctcaactgaa atcaattaaa gaactagaat ttgagaatgc actggtaatt tgtatgacat
      121 gtactgatga agtatttcga aaacattttt aaaggttgag atgtatatag atttaaccaa
      181 tcatttatct ctctcggaaa aggaatatta actaaccagc gggttatcaa aaataggtag
      241 gaaaactgta cttaatggct tatttcgcgc tgattatgag aatatcagtc tgtttgatta
      301 atttttaggg ttcaaaatac agagacaaac tccaggtcgg gtttcgccac aagcgaatta
      361 cccgatctgg agttttgtct attgcaggaa ggcgtagccg gactgcagga ataaagcccc
      421 cggtt
//
LOCUS       DAZUKZ010000304          398 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000304 DAZUKZ010000000
VERSION     DAZUKZ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.317"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..>398
                     /locus_tag="ACG8EF_003014"
     CDS             122..>398
                     /locus_tag="ACG8EF_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFS4948226.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCK"
BASE COUNT          129 a           71 c           72 g          126 t
ORIGIN      
        1 gctctatact ttttgatgtt ggtgaacttc accaacatct tttttgtata aaaaaatata
       61 gaaaagacac cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa
      121 gatgtctcat actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt
      181 cgaggaaaat tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg
      241 aactcttctg tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat
      301 cattcgttgg gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac
      361 ttatctggag ctgaagaaac aacgatttaa atgtaagt
//
LOCUS       DAZUKZ010000305          390 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000305 DAZUKZ010000000
VERSION     DAZUKZ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.318"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>390
                     /locus_tag="ACG8EF_003015"
     CDS             <1..>390
                     /locus_tag="ACG8EF_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4948227.1"
                     /translation="EQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAI
                     DYCRLYVGLVDDANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLEC
                     KCYTILLLLSRIRKIIKNMTRKKLFIIL"
BASE COUNT          165 a           36 c           68 g          121 t
ORIGIN      
        1 gaacaagagt ctattacaaa aagtttatct ccaaataagc agacggaatt taaagaattt
       61 ttaataactc taaaagctat agcatcgaaa gaagatttag aatgtcaaat cagagcaaaa
      121 cgtatggcaa ttgattattg taggctttat gtgggcttag ttgatgatgc aaatttggga
      181 attaaagaga aaagaaagta tataaaaaaa tttattgaag atgagggatt aattcaatgt
      241 tggaagggat atcctacaaa tacgctacca tttcaacaaa aattttttca taaaatgctt
      301 gaatgtaagt gttatacaat attgttgttg ttaagtagaa ttagaaagat aattaaaaat
      361 atgaccagaa aaaaattgtt tataatatta
//
LOCUS       DAZUKZ010000306          362 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000306 DAZUKZ010000000
VERSION     DAZUKZ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.320"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..312)
                     /locus_tag="ACG8EF_003016"
                     /pseudo
     CDS             complement(76..312)
                     /locus_tag="ACG8EF_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          115 a           56 c           64 g          127 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaataac gccgtttgta taattaagcg agacaaataa aaaagccatt tatgttacac
      361 tc
//
LOCUS       DAZUKZ010000307          362 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000307 DAZUKZ010000000
VERSION     DAZUKZ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.321"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>362
                     /locus_tag="ACG8EF_003017"
     CDS             <1..>362
                     /locus_tag="ACG8EF_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948228.1"
                     /translation="IKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGY
                     GIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSST
                     LLISPISTISFSPSMMFE"
BASE COUNT          134 a           50 c           53 g          125 t
ORIGIN      
        1 tataaaaaaa attcccaaaa tagatgaaaa cagtaaattg atgaaaaaca tacaatcaat
       61 ctttgggata gaagaagttt ttgtcggaga taaatgtatc ttttttgatc aacctttatt
      121 atctgatggt tatggcatag atgaaacaga tattttcgct aaagtaaata gtatattttc
      181 tggaaaaaaa gaaactttaa ttaagcttca tcctagagca aggacaaatc gttatggtga
      241 aaaaaacata ttgaattcca gtttaccgtt tgaagtcttg ctacttgaaa atgacttctc
      301 ttcaacactt ttgattagtc ctatatctac aatttctttt tcaccatcta tgatgtttga
      361 aa
//
LOCUS       DAZUKZ010000308          343 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000308 DAZUKZ010000000
VERSION     DAZUKZ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.323"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..222)
                     /locus_tag="ACG8EF_003018"
     CDS             complement(25..222)
                     /locus_tag="ACG8EF_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS4948229.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
BASE COUNT          111 a           71 c           34 g          127 t
ORIGIN      
        1 atttgcttag catttttttt cattttagca cttccctgga attgctccac ctaaaaaacc
       61 acctatagac attcctgcaa tacaagtagt tgccttggcc caatcgaccg tacatttatt
      121 tttagtgcaa tacactccat ttccataata ttttccacta tgagtggtac ccccatagat
      181 aagttgtgtc tctttaatag acaaaatttt taaatgtttc ataatctaat cacccctaaa
      241 ttaatgtttt tagactatta ttctaacatt cattaaattt caaaaaacaa ttccttcttg
      301 aaacgtagca aaataattcc tgaataagaa tttaatcact ttt
//
LOCUS       DAZUKZ010000309          343 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000309 DAZUKZ010000000
VERSION     DAZUKZ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.324"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..321)
                     /locus_tag="ACG8EF_003019"
     CDS             complement(<1..321)
                     /locus_tag="ACG8EF_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4948230.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT           96 a           55 c           63 g          129 t
ORIGIN      
        1 taaatggata atagtggttt ccaatgtatc gtttgttctt ttgtaggctg gtaaagtttg
       61 ttgtttaaac tcaccattac gatctctagg tatttccaat gttaattcac catattcggt
      121 tttgattgat cgaaagtaag aaccgtttct cgaattacct gaattaaaac cagtgcgatc
      181 atatttttcg taatctaaaa aagccgttaa ttcagtccgt aggagtgtgt ttatcgcttt
      241 ttctaagtgc gaacggaata attcatttaa atcgccttta gtgactagag tttgcacaat
      301 ttctgtagta aaatcattca tagggaagtc ctcttttctg tga
//
LOCUS       DAZUKZ010000310          338 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000310 DAZUKZ010000000
VERSION     DAZUKZ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.325"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..338
                     /locus_tag="ACG8EF_003020"
                     /pseudo
     CDS             1..338
                     /locus_tag="ACG8EF_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612034.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          142 a           59 c           64 g           73 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcc
//
LOCUS       DAZUKZ010000311          331 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000311 DAZUKZ010000000
VERSION     DAZUKZ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.327"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>331
                     /locus_tag="ACG8EF_003021"
     CDS             <1..>331
                     /locus_tag="ACG8EF_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HFS4948231.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQ"
BASE COUNT          114 a           60 c           68 g           89 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag a
//
LOCUS       DAZUKZ010000312          321 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000312 DAZUKZ010000000
VERSION     DAZUKZ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.328"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACG8EF_003022"
     CDS             complement(<1..>321)
                     /locus_tag="ACG8EF_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4948232.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAZUKZ010000313          305 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000313 DAZUKZ010000000
VERSION     DAZUKZ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.329"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8EF_003023"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8EF_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFS4948233.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           95 a           45 c           50 g          115 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgtt
//
LOCUS       DAZUKZ010000314          296 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000314 DAZUKZ010000000
VERSION     DAZUKZ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.330"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          109 a           53 c           26 g          108 t
ORIGIN      
        1 tacttttttc atataaccag aataatttaa ctattcactc taacagttaa attattttct
       61 cccatcacat gactagaagc acatcttctc aacaaacttt ttcaaaatga tcactcatat
      121 aattacacct tcattaattt aaacagcact acaaattcca ataattaatg agcatatcgt
      181 atttgtatac aaaaaaatcg ttgttaaggc aaaaatagcc gtttttacgc tcattttata
      241 tactgttgga aggtgataat ccacattact atagtttact ttttagaaaa tattat
//
LOCUS       DAZUKZ010000315          282 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000315 DAZUKZ010000000
VERSION     DAZUKZ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.331"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACG8EF_003024"
     CDS             <1..>282
                     /locus_tag="ACG8EF_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFS4948234.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAZUKZ010000316          281 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000316 DAZUKZ010000000
VERSION     DAZUKZ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.332"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..281
                     /locus_tag="ACG8EF_003025"
                     /pseudo
     CDS             1..281
                     /locus_tag="ACG8EF_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          109 a           56 c           55 g           61 t
ORIGIN      
        1 aacaaatcgt cgatctctat ctcgctggta agcctcgcgc agaaattatt cgagagtatg
       61 agcttacgcc ttcttctttc gataaatgga tgaagcaagc acaatcaacg ggctcattca
      121 aagaaagaga caacttaaca ccagaacaag cagaattgat cgcactaaga aagaaaaata
      181 agcaactcga aatggagaat gatattttaa agcaagcggc gctgatattc ggacgaaaag
      241 acaagtaatt gatgccaaca agcataaata ttccatatca g
//
LOCUS       DAZUKZ010000317          274 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000317 DAZUKZ010000000
VERSION     DAZUKZ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.denovo.333"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          113 a           30 c           49 g           82 t
ORIGIN      
        1 ttcctttaag gtgtcactaa aaattataaa aaatagtgtt gacgcacttt tgatgtcatg
       61 ttataataac gaagtcgttt ggatgagata gctaataaag cttgatttta tcgaaaaaaa
      121 cttcgaaaaa gttattgaca aataacaata aagatggtat gataatcaag tcgctgctaa
      181 tgagtggcaa agacgtttag aaaaaataaa caaagaagtt gttgacattc tcttctgaat
      241 ctgataagat taaagagttg ttaaaacaaa aaga
//
LOCUS       DAZUKZ010000318        15102 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.guided.060, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000318 DAZUKZ010000000
VERSION     DAZUKZ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.guided.060"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..577)
                     /locus_tag="ACG8EF_003026"
     CDS             complement(134..577)
                     /locus_tag="ACG8EF_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFS4948235.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACG8EF_003027"
     CDS             complement(795..2135)
                     /locus_tag="ACG8EF_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4948236.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACG8EF_003028"
     CDS             2404..3300
                     /locus_tag="ACG8EF_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS4948237.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACG8EF_003029"
     CDS             complement(3450..4391)
                     /locus_tag="ACG8EF_003029"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4948238.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ACG8EF_003030"
     CDS             4517..5638
                     /locus_tag="ACG8EF_003030"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS4948239.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG8EF_003031"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG8EF_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS4948240.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="ACG8EF_003032"
     CDS             7465..7992
                     /locus_tag="ACG8EF_003032"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4948241.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="ACG8EF_003033"
     CDS             8203..9264
                     /locus_tag="ACG8EF_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS4948242.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="ACG8EF_003034"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="ACG8EF_003034"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS4948243.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="ACG8EF_003035"
     CDS             10177..12159
                     /locus_tag="ACG8EF_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS4948244.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ACG8EF_003036"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ACG8EF_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS4948245.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="ACG8EF_003037"
     CDS             12881..14305
                     /locus_tag="ACG8EF_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFS4948246.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..>15102)
                     /locus_tag="ACG8EF_003038"
     CDS             complement(14486..>15102)
                     /locus_tag="ACG8EF_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4948247.1"
                     /translation="SATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSK
                     WITLVIYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLW
                     LILSLVLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQ
                     NQIGNEEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
BASE COUNT         4896 a         2732 c         3156 g         4318 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aa
//
LOCUS       DAZUKZ010000319        13213 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.guided.070, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000319 DAZUKZ010000000
VERSION     DAZUKZ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.guided.070"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACG8EF_003039"
     CDS             complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACG8EF_003039"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFS4948248.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="ACG8EF_003040"
     CDS             complement(2159..3031)
                     /locus_tag="ACG8EF_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4948249.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="ACG8EF_003041"
     CDS             complement(3373..4245)
                     /locus_tag="ACG8EF_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4948250.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="ACG8EF_003042"
     CDS             4373..4942
                     /locus_tag="ACG8EF_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFS4948251.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="ACG8EF_003043"
     CDS             5009..5746
                     /locus_tag="ACG8EF_003043"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4948252.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="ACG8EF_003044"
     CDS             5977..7245
                     /locus_tag="ACG8EF_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS4948253.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="ACG8EF_003045"
     CDS             7732..8196
                     /locus_tag="ACG8EF_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4948254.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="ACG8EF_003046"
     CDS             8180..8809
                     /locus_tag="ACG8EF_003046"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4948255.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACG8EF_003047"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACG8EF_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS4948256.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="ACG8EF_003048"
     CDS             10688..11134
                     /locus_tag="ACG8EF_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS4948257.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="ACG8EF_003049"
     CDS             complement(11333..11848)
                     /locus_tag="ACG8EF_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4948258.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="ACG8EF_003050"
     CDS             11965..13167
                     /locus_tag="ACG8EF_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4948259.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DAZUKZ010000320         8061 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.guided.113, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000320 DAZUKZ010000000
VERSION     DAZUKZ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.guided.113"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1564
                     /locus_tag="ACG8EF_003051"
     CDS             128..1564
                     /locus_tag="ACG8EF_003051"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4948260.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="ACG8EF_003052"
     CDS             1753..4200
                     /locus_tag="ACG8EF_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS4948261.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="ACG8EF_003053"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="ACG8EF_003053"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFS4948262.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            5487..5693
                     /locus_tag="ACG8EF_003054"
     CDS             5487..5693
                     /locus_tag="ACG8EF_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948263.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(5690..6793)
                     /locus_tag="ACG8EF_003055"
     CDS             complement(5690..6793)
                     /locus_tag="ACG8EF_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4948264.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="ACG8EF_003056"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="ACG8EF_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS4948265.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2696 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga a
//
LOCUS       DAZUKZ010000321         7285 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.guided.118, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000321 DAZUKZ010000000
VERSION     DAZUKZ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.guided.118"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..554
                     /locus_tag="ACG8EF_003057"
     CDS             <1..554
                     /locus_tag="ACG8EF_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4948266.1"
                     /translation="YEQNIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGH
                     ESPEMTSRYAHIFDETLKNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINA
                     QVLPNGYCRLPVVAGGCPHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQW
                     QRQVETNSRVKERLEQIIGSLTG"
     gene            561..938
                     /locus_tag="ACG8EF_003058"
     CDS             561..938
                     /locus_tag="ACG8EF_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFS4948267.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            1092..1874
                     /locus_tag="ACG8EF_003059"
     CDS             1092..1874
                     /locus_tag="ACG8EF_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFS4948268.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(2000..2731)
                     /gene="erm(A)"
                     /locus_tag="ACG8EF_003060"
     CDS             complement(2000..2731)
                     /gene="erm(A)"
                     /locus_tag="ACG8EF_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFS4948269.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(2789..2848)
                     /locus_tag="ACG8EF_003061"
     CDS             complement(2789..2848)
                     /locus_tag="ACG8EF_003061"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFS4948270.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            3240..3902
                     /locus_tag="ACG8EF_003062"
     CDS             3240..3902
                     /locus_tag="ACG8EF_003062"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4948271.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            4137..4280
                     /locus_tag="ACG8EF_003063"
                     /pseudo
     CDS             4137..4280
                     /locus_tag="ACG8EF_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            4430..4630
                     /locus_tag="ACG8EF_003064"
     CDS             4430..4630
                     /locus_tag="ACG8EF_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4948272.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(4884..6563)
                     /locus_tag="ACG8EF_003065"
     CDS             complement(4884..6563)
                     /locus_tag="ACG8EF_003065"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS4948273.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(6565..6777)
                     /locus_tag="ACG8EF_003066"
     CDS             complement(6565..6777)
                     /locus_tag="ACG8EF_003066"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFS4948274.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
BASE COUNT         2379 a         1194 c         1397 g         2315 t
ORIGIN      
        1 cttatgagca aaatatagtg gataaatcag gtgagattta tagattccat gcccatgcct
       61 ttcgccatac agtaggaaca agaatgatta acaacgggat gccccagcat attgtgcaga
      121 aatttttggg gcatgaaagc ccagaaatga caagcagata cgctcatatc tttgatgaaa
      181 ctctaaaaaa tgaatttact aaatttcagg aaaaactggt taccaataat ggagatgtgc
      241 ttgatctaga tgaagataat gaagtcgatg atgtagagct tcaatggttc aagaaaaata
      301 taaatgcaca agtgcttcca aatggttatt gtagattgcc agtagtagca ggtggttgtc
      361 cacatgcgaa tgcatgctta gattgcactc acttctgtac cagtaagcaa ttcttaccac
      421 agcacgaaga acagttagag cgtacagaag agttattagc catagctaag gataaacaat
      481 ggcaaagaca agtagagact aatagccgtg ttaaagagcg tttagaacaa atcattggaa
      541 gtttgacggg gtaattatca atggataaac aagttagaaa tacaacagaa attgtacgtt
      601 tggcgaagca gaaatcaaaa aagacaaggg aaaaagtaga caaagcgatt tctaaatttt
      661 cgattgaagg taaagttatt aattttaatt caatagcaaa ggaagctaat gtttctaaat
      721 catggcttta taaggaacac gatattaggc aaagaatcga atcccttcgt gagcgtcaaa
      781 taacagcaaa tgtagtctca aaacccaaga aaagttctcg ttcggaggaa atccttatta
      841 aaaccttaaa aagaagagta atggaattag aaaaagaaaa taaaaaatta cagaaccaaa
      901 ttcaaaaatt atatggagat ctgtataata aagaataatt attaatctgt agacaaattg
      961 tgaaaggatg tacttaaacg ctaacggtca gctttattga acagtcattt aggtatatgt
     1021 ccaatctagg gtaagtaaat tgagtatcaa tataaacttt atatgaacat aatcagttac
     1081 gaggtgaaat catgagcaat ttgattaacg gaaaaatacc aaatcaagcg attcaaacat
     1141 taaaaatcgt aaaagattta tttggaagtt caatagttgg agtatatcta tttggttcag
     1201 cagtaaatgg tggtttacgc attaacagcg atgtagatgt tctagtcgtc gtgaatcata
     1261 gtttacctca attaactcga aaaaaactaa cagaaagact aatgactata tcaggaaaga
     1321 ttggaaatac ggattctgtt agaccacttg aagttacggt tataaatagg agtgaagttg
     1381 tcccttggca atatcctcca aaaagagaat ttatatacgg tgagtggctc aggggtgaat
     1441 ttgagaatgg acaaattcag gaaccaagct atgatcctga tttggctatt gttttagcac
     1501 aagcaagaaa gaatagtatt tctctatttg gtcctgattc ttcaagtata cttgtctccg
     1561 tacctttgac agatattcga agagcaatta aggattcttt gccagaacta attgagggga
     1621 taaaaggtga tgagcgtaat gtaattttaa ccctagctcg aatgtggcaa acagtgacta
     1681 ctggtgaaat tacctcgaaa gatgtcgctg cagaatgggc tatacctctt ttacctaaag
     1741 agcatgtaac tttactggat atagctagaa aaggctatcg gggagagtgt gatgataagt
     1801 gggaaggact atattcaaag gtgaaagcac tcgttaagta tatgaaaaat tctatagaaa
     1861 cttctctcaa ttaggctaat tttattgcaa taacaggtgc ttacttttaa aactactgat
     1921 ttattgataa atattgaaca atttttggga agaataaagc gtcctcttgt gaaattagag
     1981 aacgctttat tactttaatt tagtgaaaca atttgtaact attgaaaata gaaagaaatt
     2041 gttccttcga tagtttatta atattagtga catttgcatg cttcaaagcc tgtcggaatt
     2101 ggtttttagt gaaaagaaca cgatattcac ggtttaccca cttataaaca aaagatcgat
     2161 actttttgta gtccttcttt gaaatcaatg gttgatgtcg ttcaagaaca atcaatacag
     2221 agtctacact tggcttagga tgaaaatata gtggtggtac ttttttgagc atttttatat
     2281 ccatctccac cattaatagt aaacccaaag ctcgttgcag attttgcaat cttttcgcaa
     2341 atcccttctc aacgataaga tagctatatt tagcctgact ttcaaaggta attcttttga
     2401 caatatccgt actgatgtta taaggaatat taccatatat cttatagttt atatgttttg
     2461 ggaaggaaaa ttttagaata tccgtttgaa tcacttttat attctcagag gggtttaccg
     2521 cttctttagt cacttgacat aagcctccat caatttctat agcagtaact gatcgactca
     2581 ttttgactag ctctttggta aaatgtcctt ttcctgatcc gatttctatt acgttgtctt
     2641 gtttactgat attcgtgtga ttcaatattt cttttacatg ctttttagaa gtaataaaat
     2701 tttgcgtgtc tttagggttt ttctggttca ttataacctt ctccttactg gttataatga
     2761 actgacatta agcgttcatt ataaccaatt aattttgatt tggttgataa cgaactttat
     2821 taataacaaa tatagaaaaa gtacccatac ttttatctcc tttccatttt tttcattatg
     2881 aatgagataa agtaatttct actactgcga tactggtgca cataattgaa acctccttat
     2941 gtaactgaat atgattgtat catcttttca ttttagaata aacctttata tgtaattttg
     3001 cacttgacac ttcagatgga cgttttcttc aaaataatat gttttgaaac ttgataagag
     3061 aagccactgt ctttactaaa aagtaaaggc tgcgataaag gacagtgact ttaataaagt
     3121 tgcactgttg tattgtcttt ggataaaatt gtctaaaggg aagttttatc cccaacgaaa
     3181 aaacatctaa agtggtaagt aatgaaagaa tgtcgataaa gtaggaatgg gggatctttt
     3241 tgaatacaat agaatcacag tttgataagg ttgcagaaga ttacgatttc gtgaatgagc
     3301 ttttgaatga ttattcattc tttgtgtcta atatgtctcc aaagaaaggc agagcattag
     3361 atatcggatg tggctcgggt ttgttagtgg agaaattagc aagttattat gatgaagtgg
     3421 tagggattga tatttctaat caaatgctcg atcttgccaa atctaaacgt caactaacaa
     3481 atacggtcta tctgaatatg aatgcagaac aacttaattt taatgagaag tttgatttta
     3541 ttgtaagccg aacaaccttt catcatttgg atgatatagc cagcgtaata cagcaaatga
     3601 aggaactgtt gaatgaggaa ggaagaatag ttattcttga taatgtatct gaagtcgaga
     3661 caccgcctac ctatgtttat aaattagggg caatccaaga gtttttaccg cattgtttca
     3721 aatttggaat aaaaaatgcg attagaattt ataatcacaa cacatctaaa tcatggcttg
     3781 agcatttagc ttcagataag tacctatctg aacaaaatta ttacgattta tatgaaaagt
     3841 tgttgcctgg atgccaattc cataaaatgg gttgggcaat gggggttgtc tggacaaaat
     3901 aacttaaagc tgtggctttg aaaagttgaa tttgtgattg ttttcagagc tatcgggtgc
     3961 tttagtataa ataacaatga ataaaaaatc agcctgtatc ttatgttact attaagtaat
     4021 ttaagataca ggttttacta tttttgaaat aagtaccttc cacttcaaat acatgtattc
     4081 aaagtgaaag tattgattta aatgattctt ttgtaattta ctcttgataa tatgaaattc
     4141 catcgcagca ggatatgaat tttaccaaaa ggatacaaaa gtgtggagaa atgatgggga
     4201 tcactgtact ggaccacttg atcatcggac gcaaacgata ttttagtttg agagaagaag
     4261 gaatgatgga agagaagtaa gttgctttct tgcaaaaaaa atgaagcaag tgtacaatta
     4321 tattgtaatt ttgtttggga atagaagttg aaacatctgt tgcacctcta tcgtacacga
     4381 gcaatgttac aatttaaatt agtgcttttg gcacgaggag gatattatta tggaaaacgg
     4441 aacagtaaaa tggttcaacg ctgaaaaagg atttggattt atctcacgtg aagatggaag
     4501 cgacgtattc gttcacttct cagctatcca aggtgacgga ttcaaaacat tagaagaagg
     4561 tcaagcagtg actttcgacg ttgaagaatc agaccgcggc ccacaagctg caaacgttgt
     4621 aaaagcttaa ttttatctgt aaatcaaatt gatggtagac atcaattttt acgagagaac
     4681 aaagcgagta acaaaaatac ctgccacaag agacaatttc tcttgtggca ggtatttttt
     4741 tgtatgaaaa agtaaataaa tttaagaggc tatgaaagag ccaccttgta tcaaggaata
     4801 agaacaaaca aaaatggctt ctcatgtttt tcatttgttt tctcttatat tcaggacaag
     4861 gtgctgattt cataacctct tatttattct tcatctggtg tcattaccat tggcaaaatc
     4921 attggatgtc tttctgtacg ttcgaataag aaaggctgaa gcgcgccagt catcgcttct
     4981 ttcaatttat attctgaaca gtcaggtcca ttcaaacatt ctcttaaagc gtggaataag
     5041 attcgctgag cttcattgat catttctcct gattctcgca tgtagacaaa tccacgagag
     5101 agaatatctg gaccagccaa gatttctttt ttcttgatat ccactgtcgc gacagctaat
     5161 acaagacctt cttctgaaag caggtgacgg tcacgtaaaa cgacattacc aatatcaccg
     5221 atcccgctac catcgatata cacatctcca gccacaaaat gcccagcagg acgcgcagaa
     5281 tcgctagtta acgctaaaac atcgccattt tccatgatga agcagttttc tgcaggaaca
     5341 cctacatctt gtgctagaga agcatgtact ttcaacatgc ggaattctcc gtggacagga
     5401 ataaaatatt ttggcttcat caaacgaagc attaattttt gttcttcttg tccaccgtga
     5461 ccggaagtat ggatgttatt gatttttccg tgaatcactt ctgcaccagc ttcagacagc
     5521 aaattgatca aatgattgac acttgttgta ttaccaggaa taggtgaact tgaaaagatc
     5581 actgtatcgt ctggctggat ttggatttga cggtgagtac cattcgcgat gcggctcaac
     5641 gcggccatcg cttctccttg agaacctgta cataggatca tcgtttcatt ggcaggcaag
     5701 cgattgattt cgtgtgcatc tacaaatgtg ccgtctggaa ctttgatata tcctaaacgt
     5761 tgaccgttga cgatggcatt ttccatgctt cgtccgaaaa cagcgatttt acgaccagtt
     5821 gccacagcgg catctgctgc ttgctgcaaa cggaagatgt tagaagcaaa gctggcaaaa
     5881 atgatacgtc cttcgatttt tgaaaagatc ttcaaaatag aatgcccaat cgttttttct
     5941 gatttagtaa atgttggaat ctcagcattt gtactatcag ataaaaggca caagactcct
     6001 tcttccccga tacgagccat tttatgcaag tttgctggtt cgccaacagg agtgaaatcg
     6061 aatttgaagt ctccagtcgc tacgatattt ccagaaggtg ttttcaccac gatccctaat
     6121 gcatctggga tactatgggt cgttctgaaa aagctgacag aagtctttct aaaacggatg
     6181 atcgtatctt cattgatctc atggagctct gcatctcgaa gcaagccatg ctcatctaat
     6241 ttgtttgtga tcaacgctaa tgcgagagga ccagcataga taggaatatt tgcttgtttc
     6301 agcaagtagg gtattccgcc gatgtggtct tcgtgcccgt gagtaatcac caaggctttt
     6361 actttgtgta agttttgtat gatgtagctg tagtcgggaa tgacatagtc aattccaagt
     6421 aaatcgtctt ccgggaattt gatcccggca tcaatcaaaa tgatttcatc ttggaattgt
     6481 accccatacg tatttttccc gatttcacct aatccaccaa tagcaaagac gcctgtctcg
     6541 ttatttttga tggaaagttt catattaaaa ctccactaat gagaaatcag cattttcttt
     6601 ttcgtactct agatgacttc cttctagaag ttgaacgaat tcaatattgt atggggtatt
     6661 ttcttccaca cgttggcgag cgtctacatc gctttttgcc tcaatgtaaa gagattttgt
     6721 ttcttcccgt ttagggttgc gtacttttgt ttcttgataa taaactttat agatcattgt
     6781 tttgtctcct ttatctgcgt aaagcagtga tttagctagt tgattttttt agacaacaca
     6841 aaacgagggg acaagccacc tggtttttca tatgctttga ctagaatact ggattcgagt
     6901 caaagagaca agcctcgttt ttgcttatct gttcggatat tttttcaacc gtaccttctg
     6961 tataaaaaat atacaacaat taattgttat tctagcataa aacagcaaat aagtatagaa
     7021 atcaactaga ttgaaagaaa aaatcaatgt ttatcatccc gctataatca taatgacttt
     7081 tttatgagag aagtgttcta ttaataggct cgttgtgcta tcatttttag taatgaaaat
     7141 gtaacaaaag attaaaagga gatgagaaag tgaaactaat gtggcattat accatgcgtt
     7201 ataagaaatt cttgtttttc aatttcatat gcgtatttgg ttttattttg attgaattag
     7261 gacttcctac gattttagca cgtat
//
LOCUS       DAZUKZ010000322         6192 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.guided.132, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000322 DAZUKZ010000000
VERSION     DAZUKZ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.guided.132"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..802
                     /locus_tag="ACG8EF_003067"
                     /pseudo
     CDS             2..802
                     /locus_tag="ACG8EF_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            1040..1276
                     /locus_tag="ACG8EF_003068"
     CDS             1040..1276
                     /locus_tag="ACG8EF_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948275.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1283..3235
                     /locus_tag="ACG8EF_003069"
     CDS             1283..3235
                     /locus_tag="ACG8EF_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS4948276.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3307..3804)
                     /gene="dfrG"
                     /locus_tag="ACG8EF_003070"
     CDS             complement(3307..3804)
                     /gene="dfrG"
                     /locus_tag="ACG8EF_003070"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS4948277.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            4079..5206
                     /locus_tag="ACG8EF_003071"
                     /pseudo
     CDS             4079..5206
                     /locus_tag="ACG8EF_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            5304..5492
                     /locus_tag="ACG8EF_003072"
     CDS             5304..5492
                     /locus_tag="ACG8EF_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFS4948278.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(5550..5903)
                     /locus_tag="ACG8EF_003073"
     CDS             complement(5550..5903)
                     /locus_tag="ACG8EF_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4948279.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
BASE COUNT         2153 a          996 c         1104 g         1939 t
ORIGIN      
        1 catgaaaatt attaatattg gagttttagc tcatgttgat gcaggaaaaa ctaccttaac
       61 agaaagctta ttatataaca gtggagcgat tacagaatta ggaagcgtgg acaaaggtac
      121 aacgaggacg gataatacgc ttttagaacg tcagagagga attacaattc agacaggaat
      181 aacctctttt cagtgggaaa atacgaaggt gaacatcata gacacgccag gacatatgga
      241 tttcttagca gaagtatatc gttcattatc agttttagat ggggcaattc tactgatttc
      301 tgcaaaagat ggcgtacaag cacaaactcg tatattattt catgcactta ggaaaatggg
      361 gattcccaca atctttttta tcaataagat tgaccaaaat ggaattgatt tatcaacggt
      421 ttatcaggat attaaagaga aactttctgc cgaaattgta atcaaacaga aggtagaact
      481 gtatcctaat atgtgtgtga cgaactttac cgaatctgaa caatgggata cggtaataga
      541 gggaaacgat gaccttttag agaaatatat gtccggtaaa tcattagaag cattggaact
      601 cgaacaagag gaaagcataa gatttcagaa ttgttctctg ttccctcttt atcatggaag
      661 tgcaaaaagt aatataggga ttgataacct tatagaagtt attactaata aattttattc
      721 atcaacacat cgaggtccgt ctgaactttg cggaaatgtt ttcaaaattg aatatacaaa
      781 aaaaagacaa cgtcttgcat attaagtagt tcagttttgg agtacaaaaa gtgaaaatgt
      841 aaacctaaat gagtatcaaa ttgcaactga atttgatact cgttttttat tgttaaatca
      901 acgtttgttg ttaatttaca ccacaaaatg taagttaaaa tgtatataaa aatgcgaacg
      961 tgaaataatt attgtaggaa tgcaaattgg tgtgttaaaa tggttacaat ttactaagca
     1021 aaaaggggtg cattttccta tgcaatttct tgagccaaaa aataagaacg ctaagtctgt
     1081 ggattgggaa atatcagagc aagtaagggt gatagttaag caatacgctg aatatgcaga
     1141 gcgtactgaa agcgaagcag tggatgaatt cttattgaac attttagacg ataagaagtt
     1201 tattgaatgg attgccaaca agcgaagtaa taagcggatt gttgaaaaaa tgggtatcaa
     1261 agatagagtg ggttgataga caatgaccaa gcttaaaaga ttagccacga aggaagatga
     1321 aattgtagat gttaaaattc cgatttcaaa cgaagaatta aaagataggg ctaaacaata
     1381 tgatttatta actccaaaac gatttgcaac aaggtataac aaaatgttgt tcttgccaac
     1441 gtcttttaaa tggaacggtt ctgaatatcc cattcaatat aattactgca tcaatccgtt
     1501 ttgttgtaat tttgggaaag aacagcacaa attcaaagat gtaaaaggga aaccaagtcg
     1561 atataaaatg actggtagtt ccaaagataa aggtcataaa ggtatgtact gcaatgacaa
     1621 tcctattggc agaggtgtat cgcaaaactg tacagtaacc cctttatcta attggtctgt
     1681 tgttgaagaa attaaacgcc ttatagaaat caatagcatc caagatgttg agccagatta
     1741 tcaatttcat aaagaaggct gctcggaaga agaatcaacc ccattcaatg agccgaagca
     1801 attttacaaa agggggaaaa gtaggggcaa atctcaacgc tatcaatgta aggcgtgtaa
     1861 gaagttcact aatgttctac caaaaagaga agaaaccact acttatcacc aacaaaagaa
     1921 caccattctt ccaatgttcg ctaagatggt tgttggaagg gtttcagtca gtcgtacttg
     1981 tgatatttta ggaattggag ttggaaccta ctatcataag ctggagtggc tgtataggcg
     2041 ttgtttggag tttttagaac gatatgagac acaacccctt caaacaaaga aatttaacga
     2101 gatgtggctt aatacggaca agatgcacta ctacctaaat aacgtccgta agaaaggaca
     2161 aggttcaaag aaatatacgg ggtttgaaga ccttaatatg caaacgtata tagttgtatc
     2221 tgctgaggtc ttatcaagat atgtgttccg ttctgatgtt gcctatgatt ggaatatttc
     2281 aatggatgaa ttaaatgaag atacaagaaa attcaaagaa gaccatctca atacttttag
     2341 tcgtaaaaat gataggttag attggtctta ttatccacaa gaaccatccg ctaacgatag
     2401 cgaaaatagg aatgcttatc ttcacgaatt agggaaaatt actaatagga gtagatttgt
     2461 cgatggttta aatgttgatg ctccttatac tacaactgct cactattggt taattaaaca
     2521 aatggttaat gccgacgaat ggcgaatgat tagtgatgat gacttctcta ttagaaacgc
     2581 cttttataga gtattcacga aagaattaag gttgagcgat gcacatcatt ttatttgcca
     2641 agttaacaag acgaaaagca gaaaacaatg cttaaaagaa ttcggacaag caaaagcaga
     2701 actgctcgat tggggagata tcagaggatt caaaacaaag tttctacgga ctttggcatc
     2761 tcattatcta actgaattac taacaagtca tcaattccac gaagaggcta ttagtaaaga
     2821 tggagaaaga tatcgcaaat atgccgataa ccctatcaaa catccattag ccactaagga
     2881 taaaggattt tattcagttg attgtagaac ggatttatct gctctggaac caaacgaaat
     2941 cgcaaagatg ctattaaatg ttaatgacca ttcgaccaat agctttattc aacagataag
     3001 acgttatata tcatctcttg aaagaccatt aacaacagct cgtggtgata agaagagcta
     3061 tatttacgct aacttcaatc cgaagtatgc tcaatttgcg attacaattc taagaactta
     3121 ctacaacttt tgtcgccctt ttaaatcagc ggataaaaag gttttgacac ctgctcaaag
     3181 attagggatt acagataaac aatttgattg gaaagatata atttatttca aatgacaaaa
     3241 agagcttgga gtaatcccaa gctcttgtgc tttatcttta ctgttcttct tcaattaacg
     3301 cacccgttaa ctcaataagt tttttctttc atatacatga aaatagtagt tatacggatt
     3361 tttatcattc tttatccctt tttgggcaaa tacctcattc cattcctcat aattcacttc
     3421 tggaaaaaaa gtatctcctt cgaattcatg atgtattttt gtgatgtaca ttttctcaac
     3481 ataagggaaa aacaaattat aaatctgttc tcctccgaaa ataaaaattt cttcttcgtt
     3541 tttacataac tcaaaaacat cttctattga atgaacaatt tcacaaccat taaaggtaaa
     3601 ccccttatct ctcgtcagaa taatatttct tctgtcaggt aaggctcttc cgattgattc
     3661 aaggttcttc ctacctaata ttatcggatg tccctttgta gtatttttaa catattccca
     3721 gtccttggga atcctccaag gaatgtcatt ctctttgcca atcactctat tcttatccat
     3781 cgcagcaatc aaagaaactt tcatttataa cacagccttt ttaactaatt taccatctaa
     3841 tttcttattt ctatcaaaaa accttttctc attatcctgc ccgttagttg aataagcaat
     3901 tttttctata agttatatta tcataatacc acaaaatcac aaattttcat tcaattatca
     3961 ttttcaattg cattttataa atcattttgt atgtaatttg gtatgtaagt taaaatgaaa
     4021 aaagtagtcc attttgaagt acattttgtc attttgtact ccaaaactga actacttact
     4081 tgcatatata cgcctttata gtggagtact acatttacga gattcggtta gagtatcaga
     4141 aaaagaaaaa ataaaagtta cagaaatgta tacttcaata aatggtgaat tatgtaagat
     4201 tgatagagct tattctggag aaattgttat tttgcaaaat gagtttttga agttaaatag
     4261 tgttcttgga gatacaaaac tattgccaca gagaaaaaag attgaaaatc cgcaccctct
     4321 actacaaaca actgttgaac cgagtaaacc tgaacagaga gaaatgttgc ttgatgccct
     4381 tttggaaatc tcagatagtg atccgcttct acgatattac gtggattcta cgacacatga
     4441 aattatactt tctttcttag ggaaagtaca aatggaagtg attagtgcac tgttgcaaga
     4501 aaagtatcat gtggagatag aactaaaaga gcctacagtc atttatatgg agagaccgtt
     4561 aaaaaatgca gaatatacca ttcacatcga agtgccgcca aatcctttct gggcttccat
     4621 tggtttatct gtatcaccgc ttccgttggg aagtggaatg cagtatgaga gctcggtttc
     4681 tcttggatac ttaaatcaat catttcaaaa tgcagttatg gaagggatac gctatggttg
     4741 cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa atctgtttta agtacggttt
     4801 atactatagc cctgttagta ctccagcaga ttttcggatg cttactccta ttgtactgga
     4861 gcaagccttt agaaaagctg gaacagaatt gttagagcca tatcttagtt ttaaagttta
     4921 tgcaccacag gaatatcttt cacgggcata taacgatgct cccaaatatt gtgcaaatat
     4981 cgtaaatact caactgaaaa ataatgaggt cattattatt ggagaaattc ctgctcgatg
     5041 tattcaagat tatcgcaatg atttaacttt ttttacaaat gggcttagtg tttgtttagc
     5101 agagctaaaa ggatatcagg ttaccactgg cgaacctgtt tgccagaccc gtcgtctaaa
     5161 tagtcggata gataaagtaa gatatatgtt caataaaata acttagtgcg ttttatgttg
     5221 ttatataaat atggtttctt attaaataag atgaaatatt ctttaatata gatttgaatt
     5281 aaagtggaaa ggaggagatt gttattataa actacaagtg gatattgtgt cctatttgtg
     5341 gaaataaaac aagactacga atacgagtgg atactatact taaaaatttc cctttatact
     5401 gccccaaatg taagaacgaa actttaatta atgttcaaaa aatgaatata ataacaatca
     5461 aagagccaga cgccaagacg cagagccgat aatttgagaa atgaaactct catcttatcg
     5521 gctctttttg tttatctgaa ttttactgac tagccttcaa tatttcttgc ttcattgata
     5581 ccttttgcta gtgcttccat taaggatagt tctttgtctg taaagctatc catgtatttt
     5641 tctacctgta accttcgagt gcttttaatc aaattatctg taggtaagaa aaattcatca
     5701 atagatacat ggagtaatga tacaagatca tacagcactt gtgtacttgg gtgttgtcct
     5761 ttgttttcta tatttgttaa ataacgtggg tcaatttcaa tcattgttcc cacttgttca
     5821 cgagtgagac cccgtttcat tcgagcctct ttaatcgcta aaccaacggc tctaaaatcg
     5881 tatttatctt cttttttacg catactaaat cacctctata cattttactg ttcctatata
     5941 accataaaca ggtatagaaa aacatgttat atagtttatg agttcctata gaacactgtg
     6001 gaatttatta atgcgatgaa tccaatttca aatagaaagg tcgctgattt tatttaccta
     6061 tacaacttta taaaatttag tcatactcct agaaagtaga taaaattggc gacttaatgg
     6121 agggaaaagt cgccatgaga aagataatct gtcatctagt tctcttaatt aagttcttat
     6181 caaaaaaact ta
//
LOCUS       DAZUKZ010000323         5614 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.guided.144, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000323 DAZUKZ010000000
VERSION     DAZUKZ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.guided.144"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..1027)
                     /locus_tag="ACG8EF_003074"
                     /pseudo
     CDS             complement(41..1027)
                     /locus_tag="ACG8EF_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1179..1874
                     /gene="vanR-A"
                     /locus_tag="ACG8EF_003075"
     CDS             1179..1874
                     /gene="vanR-A"
                     /locus_tag="ACG8EF_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFS4948280.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1852..3006
                     /locus_tag="ACG8EF_003076"
     CDS             1852..3006
                     /locus_tag="ACG8EF_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFS4948281.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3221..4189
                     /locus_tag="ACG8EF_003077"
     CDS             3221..4189
                     /locus_tag="ACG8EF_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFS4948282.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4182..5213
                     /gene="vanA"
                     /locus_tag="ACG8EF_003078"
     CDS             4182..5213
                     /gene="vanA"
                     /locus_tag="ACG8EF_003078"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFS4948283.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5219..5584
                     /locus_tag="ACG8EF_003079"
                     /pseudo
     CDS             5219..5584
                     /locus_tag="ACG8EF_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
BASE COUNT         1697 a         1091 c         1263 g         1563 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct atttccgata accgtgtatt
       61 gaaaggcatc ttcgaagcca ttatccaata tttcgcaggc ttttgtatat ttaggctggt
      121 cgatatattc accgactaag gcattcttag ctgttcgagc gagttcaata tccgtaaact
      181 taaagatcgc ttttactgct tctctaaacg gttttgaatt cttttttgga atggaactga
      241 agatgtttct taaaaaatgg acctggcatc tctgccaact tgcgttggta aatgacttac
      301 gaatcgcaga cactaggcct ttatgggcat cagaaatgat gagttctgtc ccctttaggc
      361 cgcgttcttt taagtattca aagaagatgg accatgtgtc atcactttct tcattttgaa
      421 tcatgaagcc aatgatttca cggtcgccac cttctgttat cccgatcgca atatggcagc
      481 ttttagaaag cactcgatgg tcctcacgga cttttatgta gagaacatca gtcatcagat
      541 aaggataatt cgtacctgag agtgaacggt tctgccattc gttgaccatc gggtctaact
      601 gctcagtcag gctagaaaca aaagatttcg atacagattt tccacatagc tcttcaacaa
      661 tctttgaaac tttacgtgtc gaaacgcccg aaacatacat ctcaagcatt gaagcgagca
      721 gtgccttttc atttcgctga taacgctcaa acaccgtcgg tgaaaattca ccatcacgtg
      781 ttctaggcac ttttaattcg agtgtaccca cacgagtcgt aaagtctcgc tcataatagc
      841 catttctttg actctgacga ctttctgaac gttcatagtc atcggcttga atatattctg
      901 ttcgttgatt ttccatcaat tggttgaaaa cagtggttaa aatattttta gaaacatcat
      961 cttttaccga atgttcaata atactttgaa cctcttcgtt gttcagtgta aaatgtactt
     1021 gggtcatgta acgtcctcct gggtatgttt ttgtggttaa aaacattgta ccgtaaaagg
     1081 gctgttacat ggccttttat cttttacaca attatatgga ctttatccta taatctgata
     1141 aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg
     1201 tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata
     1261 cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg
     1321 accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa
     1381 taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata
     1441 aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg
     1501 agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg
     1561 agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt
     1621 gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc
     1681 tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg
     1741 acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa
     1801 aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata
     1861 aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc
     1921 gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg
     1981 aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac
     2041 gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg
     2101 attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt
     2161 gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt
     2221 tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag
     2281 cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg
     2341 cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct
     2401 ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat
     2461 cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata
     2521 acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt
     2581 tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc
     2641 gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct
     2701 gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg
     2761 tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa
     2821 aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg
     2881 gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat
     2941 aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg
     3001 tcctaagaga tgtatataat tttttaggaa aatctcaagg ttatctttac tttttcttag
     3061 gaaattaaca atttaatatt aagaaacggc tcgttcttac acggtagact taataccgta
     3121 agaacgagcc gttttcgttc ttcagagaaa gatttgacaa gattaccatt ggcatccccg
     3181 ttttatttgg tgcctttcac agaaagggtt ggtcttaatt atgaataaca tcggcattac
     3241 tgtttatgga tgtgagcagg atgaggcaga tgcattccat gctctttcgc ctcgctttgg
     3301 cgttatggca acgataatta acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt
     3361 caatcaatgt atcagtgtgg gacataaatc agagatttcc gcctctattc ttcttgcgct
     3421 gaagagagcc ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc atatagatac
     3481 aactgctgct aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt
     3541 tgccgattat actatgatgc taattcttat ggcagtacgc aacgtaaaat cgattgtgcg
     3601 ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat
     3661 gacagttggt gtggtgggaa cgggccagat aggcaaagcg gttattgagc ggctgcgagg
     3721 atttggatgt aaagtgttgg cttatagtcg cagccgaagt atagaggtaa actatgtacc
     3781 gtttgatgag ttgctgcaaa atagcgatat cgttacgctt catgtgccgc tcaatacgga
     3841 tacgcactat attatcagcc acgaacaaat acagagaatg aagcaaggag catttcttat
     3901 caatactggg cgcggtccac ttgtagatac ctatgagttg gttaaagcat tagaaaacgg
     3961 gaaactgggc ggtgccgcat tggatgtatt ggaaggagag gaagagtttt tctactctga
     4021 ttgcacccaa aaaccaattg ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt
     4081 gataatcaca ccgcatacgg cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa
     4141 aaccattaaa aactgtttgg attttgaaag gagacaggag catgaataga ataaaagttg
     4201 caatactgtt tgggggttgc tcagaggagc atgacgtatc ggtaaaatct gcaatagaga
     4261 tagccgctaa cattaataaa gaaaaatacg agccgttata cattggaatt acgaaatctg
     4321 gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat tgctattcag
     4381 ctgtactctc gccggataaa aaaatgcacg gattacttgt taaaaagaac catgaatatg
     4441 aaatcaacca tgttgatgta gcattttcag ctttgcatgg caagtcaggt gaagatggat
     4501 ccatacaagg tctgtttgaa ttgtccggta tcccttttgt aggctgcgat attcaaagct
     4561 cagcaatttg tatggacaaa tcgttgacat acatcgttgc gaaaaatgct gggatagcta
     4621 ctcccgcctt ttgggttatt aataaagatg ataggccggt ggcagctacg tttacctatc
     4681 ctgtttttgt taagccggcg cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg
     4741 cggacgaatt ggactacgca attgaatcgg caagacaata tgacagcaaa atcttaattg
     4801 agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag
     4861 ttgttggcga ggtggaccaa atcaggctgc agtacggaat ctttcgtatt catcaggaag
     4921 tcgagccgga aaaaggctct gaaaacgcag ttataaccgt tcccgcagac ctttcagcag
     4981 aggagcgagg acggatacag gaaacggcaa aaaaaatata taaagcgctc ggctgtagag
     5041 gtctagcccg tgtggatatg tttttacaag ataacggccg cattgtactg aacgaagtca
     5101 atactctgcc cggtttcacg tcatacagtc gttatccccg tatgatggcc gctgcaggta
     5161 ttgcacttcc cgaactgatt gaccgcttga tcgtattagc gttaaagggg tgataagcat
     5221 ggaaatagga tttacttttt tagatgaaat agtacacggt gttcgttggg acgctaaata
     5281 tgccacttgg gataatttca ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt
     5341 agggacatac gagttggctg aatcgctttt gaaggcaaaa gaactggctg ctacccaagg
     5401 gtacggattg cttctatggg acggttaccg tcctaagcgt gctgtaaact gttttatgca
     5461 atgggctgca cagccggaaa ataacctgac aaaggaaagt tattatccca atattgaccg
     5521 aactgagatg atttcaaaag gatacgtggc ttcaaaatca agccatagcc gcggcggttc
     5581 tgttgcaaag ttttaaataa agaataaaat ccct
//
LOCUS       DAZUKZ010000324         2081 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.guided.212, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000324 DAZUKZ010000000
VERSION     DAZUKZ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.guided.212"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..485
                     /locus_tag="ACG8EF_003080"
                     /pseudo
     CDS             136..485
                     /locus_tag="ACG8EF_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(757..1047)
                     /locus_tag="ACG8EF_003081"
     CDS             complement(757..1047)
                     /locus_tag="ACG8EF_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4948284.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1255..1947)
                     /locus_tag="ACG8EF_003082"
     CDS             complement(1255..1947)
                     /locus_tag="ACG8EF_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4948285.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          570 a          391 c          308 g          812 t
ORIGIN      
        1 tcagttcttt gttattcgtt atttttctat tatgtttgtt catttttata ctccttctta
       61 tatttcctaa tagagtataa catagcttta agcattactt aaagataact taaaagttat
      121 cttttaaata cttaattgtt aaatacctta tttgatgcaa aagaaatcta cgatagtcaa
      181 caaaaaaatt ttcattatat ccagaaaaat ttttcttagt taaggaccta tggattgcag
      241 taatgcagaa ttcgtcgaat aaactgccaa agacatacaa tcatattgct tttaaaattg
      301 acgaacaaga tatagattcg tttgtctcaa aaatccaaat gttaggttta actgtcgaac
      361 caggacgttc tcgtgtgaaa ggcgaggctt cttcaattta cttttacgac tatgataatc
      421 acctcttcga gttacatacc ggtactctcc aggaacgttt aaaatcctac aattccacta
      481 cctgagacta gtaatgacta gtctcttttt atggcctctg atccccccat tgagcgaata
      541 ctaatagggc ttcgaattga tctgttcaat ctttaagcaa tatccctcaa atgagtttgg
      601 aaagtttagt gattcatcac aaatacttaa atacatgtag ttatatatag attgaggttc
      661 ttgtgagcgt atgctcgtgt gggttcaaat cccatcgtct gcatccatct ttccaaaaca
      721 agcggctagg acatttttgt ctcagccgct tgttttctag tttattcttt catcttgtcg
      781 gacctttttt cttgggtata gacttttctc atattctacc acaattttgg gtttccctaa
      841 cacatgatag gatctcacaa caaattgctt tctattatta taataacctg taacgactaa
      901 cttcatccct tcgtctactt cagctaggaa gttcaaacta tgggaagcaa tcaaacaatt
      961 gaccccattt aaactgaaac ggatcaacgg tctggcacta acttgcaaag tacggatctt
     1021 atctactgtt ccatattttt tctccataac cttcacctca ataccattat acgaacatac
     1081 attccctttg taaagaagaa actctgtttt atctttttga ctttttgtct attacctttc
     1141 ttaaaatcat attggcactt cctgcgaaga tgaaggacat gtactaaaaa aaagaggcag
     1201 cagtatagat tactgttgct tctttactaa tttaggttta cttgttaaga aagattattt
     1261 acgcaaatgt tcttcaacaa tttgctgaga tttatcagct gtatcttcca cttcatcttt
     1321 tgctttttct aacttttgaa tatattgttt caagcttgag ttttcagcag ttgcttcgtc
     1381 taatttcttt tgtgtatctg ttaactgagt ttctacattt gttttttctt gtgtcagtgt
     1441 tgcgatttgt tgattcaaac catttacagt attttgtaat tctgaaagtt ttgcttgtgc
     1501 ttcttgtagt cttgctgtcc aatctgcatt cagatgattg atttcatcat ttttttggtt
     1561 gatctcatct agtttgacat tgatttcatt ttgtttgctt tgtagttgtg catccttgct
     1621 gactaattga ctttctaagt tttgtttttc tgctgccgat tgattttttt ggttttctaa
     1681 ttcatttact tttgtttggt attcttggac atctgccttt gaatcttgca atgtctgttc
     1741 tttttgtgct agtcgagcgg taagatctgt aagcgcttgc tctttatctg tcaatgtatg
     1801 atcaagctgt gtcagattct cattgattgt atcaatatct gtctctccac cccacaaaat
     1861 aatttcattt gcgtaccgcg tgcctgtgta gccaattaga catagaaatg caacgacaat
     1921 cccaccagta ataaatttct ttttcatttg catagctcct taatccaata cttttacatg
     1981 ctaaaagtaa gtgatctcaa acacaatgtc aagcgttttt aaattacatg tgtaatttta
     2041 atataaatac tatcaaatac tcatttaatc ctcctttttt a
//
LOCUS       DAZUKZ010000325         1504 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.guided.237, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000325 DAZUKZ010000000
VERSION     DAZUKZ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.guided.237"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            262..345
                     /locus_tag="ACG8EF_003083"
                     /pseudo
     CDS             262..345
                     /locus_tag="ACG8EF_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            394..477
                     /locus_tag="ACG8EF_003084"
     CDS             394..477
                     /locus_tag="ACG8EF_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFS4948286.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="ACG8EF_003085"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="ACG8EF_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS4948287.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAZUKZ010000326         1421 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.guided.242, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000326 DAZUKZ010000000
VERSION     DAZUKZ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.guided.242"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1350
                     /locus_tag="ACG8EF_003086"
     CDS             <1..1350
                     /locus_tag="ACG8EF_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFS4948288.1"
                     /translation="RDFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQ
                     IYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANA
                     VILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMK
                     YLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLN
                     TNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFL
                     RQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVF
                     EGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFG
                     EDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          597 a          114 c          239 g          471 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg aatcattaaa aaaacattat
       61 acagagcctt gggaagatga agtttttaga gtaattattg aatataacaa tgttcctatt
      121 ggatatggac aaatatataa aatgtatgat gagttatata ctgattatca ttatccaaaa
      181 actgatgaga tagtctatgg tatggatcaa tttataggag agccaaatta ttggagtaaa
      241 ggaattggta caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct
      301 aatgcagtta ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa
      361 tctggtttta gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa
      421 gattgttatt taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa
      481 tatttaattg agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt
      541 ggttatgata gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt
      601 actaataaga aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt aaatacaaat
      661 ttagaaacta atgtaaaaat tcctaatatt gaatattcgt atattagtga tgaattatct
      721 atactaggtt ataaagaaat taaaggaact tttttaacac cagaaattta ttctactatg
      781 tcagaagaag aacaaaattt gttaaaacga gatattgcca gttttttaag acaaatgcac
      841 ggtttagatt atacagatat tagtgaatgt actattgata ataaacaaaa tgtattagaa
      901 gagtatatat tgttgcgtga aactatttat aatgatttaa ctgatataga aaaagattat
      961 atagaaagtt ttatggaaag actaaatgca acaacagttt ttgagggtaa aaagtgttta
     1021 tgccataatg attttagttg taatcatcta ttgttagatg gcaataatag attaactgga
     1081 ataattgatt ttggagattc tggaattata gatgaatatt gtgattttat atacttactt
     1141 gaagatagtg aagaagaaat aggaacaaat tttggagaag atatattaag aatgtatgga
     1201 aatatagata ttgagaaagc aaaagaatat caagatatag ttgaagaata ttatcctatt
     1261 gaaactattg tttatggaat taaaaatatt aaacaggaat ttatcgaaaa tggtagaaaa
     1321 gaaatttata aaaggactta taaagattga ttatataata tatgaaaagc tattataaaa
     1381 gacggttctg ttgcaaagtt ttaaatctac tatcaaataa g
//
LOCUS       DAZUKZ010000327         1350 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5226
            SAMEA115707582-rid22570583.guided.250, whole genome shotgun
            sequence.
ACCESSION   DAZUKZ010000327 DAZUKZ010000000
VERSION     DAZUKZ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707582
            Sequence Read Archive: ERR13197310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288075.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288075.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:10:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,088
            CDSs (total)                      :: 3,024
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 58 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 12 of 100
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707582-rid22570583.guided.250"
                     /isolate="VRE5226"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..298
                     /locus_tag="ACG8EF_003087"
                     /pseudo
     CDS             47..298
                     /locus_tag="ACG8EF_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="ACG8EF_003088"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="ACG8EF_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS4948289.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
